title: Serotyping of foot and mouth disease virus and Pasteurella multocida from Indian gaurs (Bos gaurus), concurrently infected with foot and mouth disease and haemorrhagic septicaemia.
abstract: We report the serotyping of foot-and-mouth disease virus (FMDV) and Pasteurella multocida from Indian gaurs which were concurrently infected with foot-and-mouth disease (FMD) and haemorrhagic septicaemia. Bannerghatta biological park (BBP), a national park located in the outskirts of Bengaluru city, Karnataka, India, is bordered by several villages. These villages witnessed massive outbreaks of FMD which spread rapidly to the herbivores at BBP. Post-mortem was conducted on carcasses of two Indian gaurs that died with symptoms of FMD. The salient gross findings included extensive vesicular lesions on the tongue, gums, cheeks, upper palate and hooves. Haemorrhagic tracheitis and ecchymotic haemorrhages on the heart were characteristic. The vesicular lesions of oral cavity were positive for 'O' type of FMD virus by sandwich enzyme-linked immuno sorbent assay (ELISA). The heart blood and spleen samples yielded growth of pure cultures of P. multocida. The isolates were typed as P. multocida type B using KTSP61 and KTT72 primers yielding specific amplicons of 620 bp. The phylogenetic analysis of the isolates was carried by sequencing of 1.4-Kbp nucleotides on the 16S ribosomal RNA (rRNA) gene of the isolates.
authors.affiliation: Institute of Animal Health and Veterinary Biologicals, Hebbal, Bengaluru, 560 024, Karnataka, India, dinglychandru@yahoo.com.
authors.forename: Basavegowdanadoddi Marinaik
authors.initials: BM
authors.lastname: Chandranaik
authors.forename: Raveendra
authors.initials: R
authors.lastname: Hegde
authors.forename: Beechagondahalli Papanna
authors.initials: BP
authors.lastname: Shivashankar
authors.forename: Papanna
authors.initials: P
authors.lastname: Giridhar
authors.forename: Handenahally Kaverappa
authors.initials: HK
authors.lastname: Muniyellappa
authors.forename: Rajeshwar
authors.initials: R
authors.lastname: Kalge
authors.forename: Benamanahalli Raju
authors.initials: BR
authors.lastname: Sumathi
authors.forename: Kumble
authors.initials: K
authors.lastname: Nithinprabhu
authors.forename: Narasimhaiah
authors.initials: N
authors.lastname: Chandrashekara
authors.forename: Venkataramanappa
authors.initials: V
authors.lastname: Manjunatha
authors.forename: Nirupama
authors.initials: N
authors.lastname: Jaisingh
authors.forename: Asha
authors.initials: A
authors.lastname: Mayanna
authors.forename: Gowda Kallenahalli
authors.initials: GK
authors.lastname: Chandrakala
authors.forename: Sermaraja
authors.initials: S
authors.lastname: Kanaka
authors.forename: Mudalagiri Dasappagupta
authors.initials: MD
authors.lastname: Venkatesha
mesh_terms: D000818:Animals; D016164:Bison; D017931:DNA Primers / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005536:Foot-and-Mouth Disease / Q000150:complications / Q000453:epidemiology* / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics / Q000302:isolation & purification*; D006483:Hemorrhagic Septicemia / Q000150:complications / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D007194:India / Q000453:epidemiology; D016979:Pasteurella multocida / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012703:Serotyping
chemical_list: D017931:DNA Primers; D012336:RNA, Ribosomal, 16S