title: Activity Assay of Glutathione S-Transferase (GSTs) Enzyme as a Diagnostic Biomarker for Liver Hydatid Cyst in Vitro.
abstract: BACKGROUND
  The aim of this study was to detect the Glutathione S-Transferase(GST) enzyme activity of healthy / cystic liver as a diagnostic biomarker for hydatidosis. In order to compare with liver tissue, the level of the GSTs enzyme activity of parasite was also determined.
  
  
  METHODS
  Parasites were collected from sheep liver tissue with hydatid cysts at a local abattoir and washed with PBS buffer. Collected parasites and liver tissues were sonicated or homogenized respectively. Extract solution samples were centrifuged and stored at - 20°C. GST enzyme activities were measured in the extract of parasite and liver tissue samples (healthy and infected livers). Protein amounts and protein bands were detected using Bradford and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) methods respectively. To determine significant difference between two groups, two-sample t-test was performed.
  
  
  RESULTS
  GST specific activities of healthy / infected livers and parasites were estimated 304, 1297 and 146 U/ml/mg respectively. Significant higher GST specific activities in cystic liver than healthy liver was observed (P <0.05). T-test analysis showed GST activity of parasite was lower than healthy liver tissue. SDS-PAGE showed GST protein bands with 24 kDa in parasite samples and25 kDa in liver tissues.
  
  
  CONCLUSIONS
  GST activity incystic liver tissue could be concerned as a biomarker for hydatid cyst diagnosis with other hydatid disease parameters.
authors.affiliation: 1. Dept. of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Iran.
authors.forename: Lila
authors.initials: L
authors.lastname: Moatamedi Pour
authors.affiliation: 1. Dept. of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Farahnak
authors.affiliation: 1. Dept. of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Iran.
authors.forename: Mohamadbagher
authors.initials: M
authors.lastname: Molaei Rad
authors.affiliation: 2. Dept. of Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Taghi
authors.initials: T
authors.lastname: Golmohamadi
authors.affiliation: 3. Dept. of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mohamadreza
authors.initials: M
authors.lastname: Eshraghian
keywords: Glutathione S-Transferase; Hydatid cyst; Liver; Parasite; Protoscolices