title: Standalone cohesin as a molecular shuttle in cellulosome assembly.
abstract: The cellulolytic bacterium Ruminococcus flavefaciens of the herbivore rumen produces an elaborate cellulosome system, anchored to the bacterial cell wall via the covalently bound scaffoldin ScaE. Dockerin-bearing scaffoldins also bind to an autonomous cohesin of unknown function, called cohesin G (CohG). Here, we demonstrate that CohG binds to the scaffoldin-borne dockerin in opposite orientation on a distinct site, relative to that of ScaE. Based on these structural data, we propose that the complexed dockerin is still available to bind ScaE on the cell surface. CohG may thus serve as a molecular shuttle for delivery of scaffoldins to the bacterial cell surface.
authors.affiliation: Department of Molecular Microbiology and Biotechnology, Tel Aviv University, 69978, Israel; The Daniella Rich Institute for Structural Biology, Tel Aviv University, 69978, Israel.
authors.forename: Milana
authors.initials: M
authors.lastname: Voronov-Goldman
authors.affiliation: Department of Molecular Microbiology and Biotechnology, Tel Aviv University, 69978, Israel; The Daniella Rich Institute for Structural Biology, Tel Aviv University, 69978, Israel.
authors.forename: Oren
authors.initials: O
authors.lastname: Yaniv
authors.affiliation: Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel.
authors.forename: Ozgur
authors.initials: O
authors.lastname: Gul
authors.affiliation: Department of Molecular Microbiology and Biotechnology, Tel Aviv University, 69978, Israel; The Daniella Rich Institute for Structural Biology, Tel Aviv University, 69978, Israel.
authors.forename: Hagar
authors.initials: H
authors.lastname: Yoffe
authors.affiliation: Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel.
authors.forename: Orly
authors.initials: O
authors.lastname: Salama-Alber
authors.affiliation: Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel.
authors.forename: Michal
authors.initials: M
authors.lastname: Slutzki
authors.affiliation: Department of Molecular Microbiology and Biotechnology, Tel Aviv University, 69978, Israel; The Daniella Rich Institute for Structural Biology, Tel Aviv University, 69978, Israel.
authors.forename: Maly
authors.initials: M
authors.lastname: Levy-Assaraf
authors.affiliation: Department of Molecular Microbiology and Biotechnology, Tel Aviv University, 69978, Israel; Faculty of Agriculture, Meijo University, Nagoya 468-8502, Japan.
authors.forename: Sadanari
authors.initials: S
authors.lastname: Jindou
authors.affiliation: Department of Chemical Research Support, The Weizmann Institute of Science, Rehovot 76100, Israel.
authors.forename: Linda J W
authors.initials: LJ
authors.lastname: Shimon
authors.affiliation: Department of Molecular Microbiology and Biotechnology, Tel Aviv University, 69978, Israel.
authors.forename: Ilya
authors.initials: I
authors.lastname: Borovok
authors.affiliation: Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel. Electronic address: ed.bayer@weizmann.ac.il.
authors.forename: Edward A
authors.initials: EA
authors.lastname: Bayer
authors.affiliation: Department of Molecular Microbiology and Biotechnology, Tel Aviv University, 69978, Israel; The Daniella Rich Institute for Structural Biology, Tel Aviv University, 69978, Israel. Electronic address: lamedr@post.tau.ac.il.
authors.forename: Raphael
authors.initials: R
authors.lastname: Lamed
authors.affiliation: Department of Molecular Microbiology and Biotechnology, Tel Aviv University, 69978, Israel; The Daniella Rich Institute for Structural Biology, Tel Aviv University, 69978, Israel.
authors.forename: Felix
authors.initials: F
authors.lastname: Frolow
mesh_terms: D000595:Amino Acid Sequence; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000378:metabolism*; D044603:Cellulosomes / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry / Q000378:metabolism*; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D000097722:Cohesins
chemical_list: D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D000097722:Cohesins
keywords: Cellulose degradation; Cohesin; Cohesin–dockerin complex; Dockerin; Rumen bacteria; Ruminococcus flavefaciens; Type III