title: Comparison of manual and automated cultures of bone marrow stromal cells for bone tissue engineering.
abstract: The development of an automated cell culture system would allow stable and economical cell processing for wider clinical applications in the field of regenerative medicine. However, it is crucial to determine whether the cells obtained by automated culture are comparable to those generated by manual culture. In the present study, we focused on the primary culture process of bone marrow stromal cells (BMSCs) for bone tissue engineering and investigated the feasibility of its automation using a commercially available automated cell culture system in a clinical setting. A comparison of the harvested BMSCs from manual and automated cultures using clinically acceptable protocols showed no differences in cell yields, viabilities, surface marker expression profiles, and in vivo osteogenic abilities. Cells cultured with this system also did not show malignant transformation and the automated process was revealed to be safe in terms of microbial contamination. Taken together, the automated procedure described in this report provides an approach to clinical bone tissue engineering.
authors.affiliation: Medical Device Development Laboratories, Kaneka Corporation, 1-8 Miyamae-cho, Takasago-cho, Takasago, Hyogo 676-8688, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Akiyama
authors.affiliation: Tissue Engineering Research Group, Division of Molecular Therapy, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
authors.forename: Asako
authors.initials: A
authors.lastname: Kobayashi
authors.affiliation: Medical Device Development Laboratories, Kaneka Corporation, 1-8 Miyamae-cho, Takasago-cho, Takasago, Hyogo 676-8688, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Ichimura
authors.affiliation: Technology Management Department, Medical Devices Division, Kaneka Corporation, 2-3-18 Nakanoshima, Kita-ku, Osaka 530-8288, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tone
authors.affiliation: Medical Device Development Laboratories, Kaneka Corporation, 1-8 Miyamae-cho, Takasago-cho, Takasago, Hyogo 676-8688, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Nakatani
authors.affiliation: Tissue Engineering Research Group, Division of Molecular Therapy, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
authors.forename: Minoru
authors.initials: M
authors.lastname: Inoue
authors.affiliation: Tissue Engineering Research Group, Division of Molecular Therapy, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
authors.forename: Arinobu
authors.initials: A
authors.lastname: Tojo
authors.affiliation: Tissue Engineering Research Group, Division of Molecular Therapy, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan; Department of Oral and Maxillofacial Surgery, Matsumoto Dental University School of Dentistry, 1780 Hirookagobara, Shiojiri, Nagano 399-0781, Japan. Electronic address: kagami@ims.u-tokyo.ac.jp.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Kagami
mesh_terms: D000328:Adult; D001331:Automation / Q000379:methods*; D001854:Bone Marrow Cells / Q000166:cytology*; D001842:Bone and Bones / Q000166:cytology*; D018929:Cell Culture Techniques / Q000379:methods*; D002470:Cell Survival; D002471:Cell Transformation, Neoplastic; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010012:Osteogenesis; D044968:Regenerative Medicine; D023822:Tissue Engineering
keywords: Automated cell culture system; Bone marrow stromal cells; Bone tissue engineering; Osteogenic potential; Regenerative medicine