title: Chemoenzymatic Synthesis of (36)S Isotopologues of Methionine and S-Adenosyl-L-methionine.
abstract: Substrates containing isotope labels at specific atoms are required for transition-state analysis based on the measurement of multiple kinetic isotope effects.(36)S-labeled l-methionine and S-adenosyl-l-methionine were synthesized from elemental sulfur using a chemoenzymatic approach with >98% (36)S enrichment. This method provides access to previously inaccessible sulfur isotope-labeled substrates for sulfur kinetic isotope effect studies.
authors.affiliation: Department of Biochemistry, Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Avenue, Bronx, New York 10461, United States.
authors.forename: Myles B
authors.initials: MB
authors.lastname: Poulin
authors.affiliation: Department of Biochemistry, Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Avenue, Bronx, New York 10461, United States.
authors.forename: Quan
authors.initials: Q
authors.lastname: Du
authors.affiliation: Department of Biochemistry, Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Avenue, Bronx, New York 10461, United States.
authors.forename: Vern L
authors.initials: VL
authors.lastname: Schramm
mesh_terms: D007553:Isotope Labeling / Q000379:methods*; D007700:Kinetics; D008715:Methionine / Q000138:chemical synthesis* / Q000737:chemistry; D015394:Molecular Structure; D012436:S-Adenosylmethionine / Q000138:chemical synthesis* / Q000737:chemistry; D013462:Sulfur Radioisotopes / Q000737:chemistry*
chemical_list: D013462:Sulfur Radioisotopes; D012436:S-Adenosylmethionine; D008715:Methionine