title: Inhibiting IκBβ-NFκB signaling attenuates the expression of select pro-inflammatory genes.
abstract: Multiple mediators of septic shock are regulated by the transcription factor nuclear factor κB (NFκB). However, complete NFκB inhibition can exacerbate disease, necessitating evaluation of targeted strategies to attenuate the pro-inflammatory response. Here, we demonstrate that in murine macrophages, low-dose NFκB inhibitors specifically attenuates lipopolysaccharide (LPS)-induced IκBβ degradation and the expression of a select subset of target genes (encoding IL1β, IL6, IL12β). Gain- and loss-of-function experiments demonstrate the necessary and sufficient role of inhibitor of NFκB family member IκBβ (also known as NFKBIB) in the expression of these genes. Furthermore, both fibroblasts and macrophages isolated from IκBβ overexpressing mice demonstrate attenuated LPS-induced IκBβ-NFκB signaling and IL1β, IL6 and IL12β expression. Further confirming the role of IκBβ and its NFκB subunit binding partner cRel in LPS-induced gene expression, pre-treatment of wild-type mouse embryonic fibroblasts with a cell-permeable peptide containing the cRel nuclear localization sequence attenuated IL6 expression. We prove that LPS-induced IκBβ-NFκB signaling can be selectively modulated to attenuate the expression of select pro-inflammatory target genes, thus providing therapeutic insights for patients exposed to systemic inflammatory stress.
authors.affiliation: Section of Neonatology, Department of Pediatrics, University of Colorado School of Medicine, Aurora, CO 80045, USA sarah.mckenna@ucdenver.edu clyde.wright@ucdenver.edu.
authors.forename: Sarah
authors.initials: S
authors.lastname: McKenna
authors.affiliation: Section of Neonatology, Department of Pediatrics, University of Colorado School of Medicine, Aurora, CO 80045, USA sarah.mckenna@ucdenver.edu clyde.wright@ucdenver.edu.
authors.forename: Clyde J
authors.initials: CJ
authors.lastname: Wright
mesh_terms: D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects / Q000235:genetics; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects / Q000235:genetics*; D020672:I-kappa B Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics*; D007249:Inflammation / Q000235:genetics*; D007378:Interleukins / Q000235:genetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000235:genetics*; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics*
chemical_list: C083487:I kappa B beta protein; D020672:I-kappa B Proteins; D007378:Interleukins; D008070:Lipopolysaccharides; D016328:NF-kappa B
keywords: Innate immune response; IκB proteins; LPS; Lipopolysaccharide; NFκB