title: Cryo-electron microscopy for structural biology: current status and future perspectives.
abstract: Recently, significant technical breakthroughs in both hardware equipment and software algorithms have enabled cryo-electron microscopy (cryo-EM) to become one of the most important techniques in biological structural analysis. The technical aspects of cryo-EM define its unique advantages and the direction of development. As a rapidly emerging field, cryo-EM has benefitted from highly interdisciplinary research efforts. Here we review the current status of cryo-EM in the context of structural biology and discuss the technical challenges. It may eventually merge structural and cell biology at multiple scales.
authors.affiliation: School of Life Sciences, Tsinghua University, Beijing, 100084, China, hongweiwang@tsinghua.edu.cn.
authors.forename: HongWei
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D020285:Cryoelectron Microscopy / Q000379:methods* / Q000639:trends; D006801:Humans; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional; D046911:Macromolecular Substances / Q000737:chemistry* / Q000648:ultrastructure*; D008958:Models, Molecular; D008968:Molecular Conformation; D015203:Reproducibility of Results; D050916:TRPV Cation Channels / Q000737:chemistry / Q000648:ultrastructure
chemical_list: D046911:Macromolecular Substances; D050916:TRPV Cation Channels