title: Analysis of amicarbazone and its two metabolites in grains and soybeans by liquid chromatography with tandem mass spectrometry.
abstract: A sensitive, simple and reliable analytical method based on a modified quick, easy, cheap, effective, rugged, safe sample preparation and liquid chromatography with tandem mass spectrometry detection was developed for the simultaneous determination of amicarbazone and its two major metabolites desamino amicarbazone and isopropyl-2-hydroxy-desamino amicarbazone residues in grains (rice, wheat, corn, buckwheat) and soybean. Several parameters, including liquid chromatography and tandem mass spectrometry conditions, extraction approaches and the adsorbents for clean-up, which might influence the accuracy of the method, were extensively investigated. The established method was further validated by determining the linearity (R(2) > 0.99), fortified recovery (79-118%), precision (1-12%) and sensitivity (limit of quantification, 5 μg/kg for amicarbazone and desamino amicarbazone, and 10 μg/kg for isopropyl-2-hydroxy-desamino amicarbazone). Finally, the established method was successfully applied to determine the residues of amicarbazone and its metabolites in 49 real samples of grain and soybean.
authors.affiliation: Institute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai, P. R. China.
authors.forename: Maofeng
authors.initials: M
authors.lastname: Dong
authors.affiliation: Institute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai, P. R. China.
authors.forename: Dongxia
authors.initials: D
authors.lastname: Nie
authors.affiliation: Institute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai, P. R. China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Tang
authors.affiliation: Institute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai, P. R. China.
authors.forename: Qinxiong
authors.initials: Q
authors.lastname: Rao
authors.affiliation: Institute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai, P. R. China.
authors.forename: Mingqing
authors.initials: M
authors.lastname: Qu
authors.affiliation: Institute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai, P. R. China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of College of Science, China Agricultural University, Beijing, P. R. China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Han
authors.affiliation: Institute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai, P. R. China.
authors.forename: Weiguo
authors.initials: W
authors.lastname: Song
authors.affiliation: Institute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai, P. R. China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Han
keywords: Amicarbazone; Grains; Liquid chromatography with tandem mass spectrometry; Metabolites