title: Protein recovery from inclusion bodies of Escherichia coli using mild solubilization process.
abstract: Formation of inclusion bodies in bacterial hosts poses a major challenge for large scale recovery of bioactive proteins. The process of obtaining bioactive protein from inclusion bodies is labor intensive and the yields of recombinant protein are often low. Here we review the developments in the field that are targeted at improving the yield, as well as quality of the recombinant protein by optimizing the individual steps of the process, especially solubilization of the inclusion bodies and refolding of the solubilized protein. Mild solubilization methods have been discussed which are based on the understanding of the fact that protein molecules in inclusion body aggregates have native-like structure. These methods solubilize the inclusion body aggregates while preserving the native-like protein structure. Subsequent protein refolding and purification results in high recovery of bioactive protein. Other parameters which influence the overall recovery of bioactive protein from inclusion bodies have also been discussed. A schematic model describing the utility of mild solubilization methods for high throughput recovery of bioactive protein has also been presented.
authors.affiliation: Product Development Cell, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, 110067, India. anupamsinghsls@gmail.com.
authors.forename: Anupam
authors.initials: A
authors.lastname: Singh
authors.affiliation: Product Development Cell, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, 110067, India. vaibhav@nii.ac.in.
authors.forename: Vaibhav
authors.initials: V
authors.lastname: Upadhyay
authors.affiliation: Product Development Cell, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, 110067, India. arunkumarupadhyay@gmail.com.
authors.forename: Arun Kumar
authors.initials: AK
authors.lastname: Upadhyay
authors.affiliation: Product Development Cell, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, 110067, India. smsingh.79@gmail.com.
authors.forename: Surinder Mohan
authors.initials: SM
authors.lastname: Singh
authors.affiliation: Product Development Cell, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, 110067, India. amulya@nii.res.in.
authors.forename: Amulya Kumar
authors.initials: AK
authors.lastname: Panda
mesh_terms: D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D002479:Inclusion Bodies / Q000378:metabolism*; D008958:Models, Molecular; D011489:Protein Denaturation; D017510:Protein Folding; D058849:Protein Refolding; D058767:Protein Unfolding; D011994:Recombinant Proteins / Q000737:chemistry* / Q000378:metabolism*; D012995:Solubility
chemical_list: D011994:Recombinant Proteins