title: "Different trend" in multiple primary lung cancer and intrapulmonary metastasis.
abstract: BACKGROUND
  The distinguishing of intrapulmonary metastatic tumors from multiple primary lung cancers is difficult but of great importance for the therapeutic management and prognosis of these patients.
  
  
  METHODS
  We used genomic DNA analyzed by six microsatellites (D7S1824, D15S822, D2S1363, D10S1239, D6S1056, and D22S689) with PCR to identify discordant allelic variation from 12 patients. There are five patients with multiple primary lung cancers and seven patients who were diagnosed with intrapulmonary metastases from 850 patients with primary lung cancer in our hospital. The experiments were approved by the West China Hospital Ethics committee (No. 2013 (33)) and all patients agreed to participate in the study and signed an informed consent form.
  
  
  RESULTS
  In the group of metachronous lung tumor, three of five patients have different histological types and one of five patients have the same histological type which showed "contradictory trend". The other one showed "unique trend". In the second group (intrapulmonary metastasis lung tumor), one patient showed "contradictory trend" and the others showed "unique trend".
  
  
  CONCLUSIONS
  "Different trends" are useful in discrimination of intrapulmonary metastasis lung cancer and multiple primary lung cancer even diagnosed with the histopathological evaluation.
authors.affiliation: Department of Thoracic Surgery, West-China Hospital, Sichuan University, Chengdu, 610041, China. shencheng568@yahoo.com.
authors.forename: Cheng
authors.initials: C
authors.lastname: Shen
authors.affiliation: Department of Thoracic Surgery, West-China Hospital, Sichuan University, Chengdu, 610041, China. herojerryhero@me.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Thoracic Surgery, West-China Hospital, Sichuan University, Chengdu, 610041, China. tianlong@yahoo.com.
authors.forename: Long
authors.initials: L
authors.lastname: Tian
authors.affiliation: Department of Thoracic Surgery, West-China Hospital, Sichuan University, Chengdu, 610041, China. zhouyubin@yahoo.com.
authors.forename: Yubin
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Thoracic Surgery, West-China Hospital, Sichuan University, Chengdu, 610041, China. chendali@yahoo.com.
authors.forename: Dali
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Thoracic Surgery, West-China Hospital, Sichuan University, Chengdu, 610041, China. duheng@yahoo.com.
authors.forename: Heng
authors.initials: H
authors.lastname: Du
authors.affiliation: Department of Pathology, West-China Hospital, Sichuan University, Chengdu, 610041, China. wangweiya@yahoo.com.
authors.forename: Weiya
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Thoracic Surgery, West-China Hospital, Sichuan University, Chengdu, 610041, China. liulunxu@yahoo.com.
authors.forename: Lunxu
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Thoracic Surgery, West-China Hospital, Sichuan University, Chengdu, 610041, China. cheguowei_hx@aliyun.com.
authors.forename: Guowei
authors.initials: G
authors.lastname: Che
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics*; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000556:secondary; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D009378:Neoplasms, Multiple Primary / Q000175:diagnosis / Q000235:genetics*; D011110:Polymorphism, Genetic
chemical_list: D014408:Biomarkers, Tumor