title: Bacterial communities in haloalkaliphilic sulfate-reducing bioreactors under different electron donors revealed by 16S rRNA MiSeq sequencing.
abstract: Biological technology used to treat flue gas is useful to replace conventional treatment, but there is sulfide inhibition. However, no sulfide toxicity effect was observed in haloalkaliphilic bioreactors. The performance of the ethanol-fed bioreactor was better than that of lactate-, glucose-, and formate-fed bioreactor, respectively. To support this result strongly, Illumina MiSeq paired-end sequencing of 16S rRNA gene was applied to investigate the bacterial communities. A total of 389,971 effective sequences were obtained and all of them were assigned to 10,220 operational taxonomic units (OTUs) at a 97% similarity. Bacterial communities in the glucose-fed bioreactor showed the greatest richness and evenness. The highest relative abundance of sulfate-reducing bacteria (SRB) was found in the ethanol-fed bioreactor, which can explain why the performance of the ethanol-fed bioreactor was the best. Different types of SRB, sulfur-oxidizing bacteria, and sulfur-reducing bacteria were detected, indicating that sulfur may be cycled among these microorganisms. Because high-throughput 16S rRNA gene paired-end sequencing has improved resolution of bacterial community analysis, many rare microorganisms were detected, such as Halanaerobium, Halothiobacillus, Desulfonatronum, Syntrophobacter, and Fusibacter. 16S rRNA gene sequencing of these bacteria would provide more functional and phylogenetic information about the bacterial communities.
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing 100190, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors.forename: Jiemin
authors.initials: J
authors.lastname: Zhou
authors.affiliation: 101 Institute, Ministry of Civil Affairs, Beijing 100070, PR China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Zhou
authors.affiliation: 101 Institute, Ministry of Civil Affairs, Beijing 100070, PR China.
authors.forename: Yuguang
authors.initials: Y
authors.lastname: Li
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing 100190, PR China. Electronic address: jmxing@ipe.ac.cn.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Xing
mesh_terms: D019149:Bioreactors; D000431:Ethanol; D005947:Glucose; D059013:Microbial Consortia; D010084:Oxidation-Reduction; D012336:RNA, Ribosomal, 16S / Q000737:chemistry*; D013431:Sulfates / Q000378:metabolism*
chemical_list: D012336:RNA, Ribosomal, 16S; D013431:Sulfates; D000431:Ethanol; D005947:Glucose
keywords: Bacterial community; Haloalkaliphilic; MiSeq; Sulfate reduction