title: Considerations in the improvement of human epidermal keratinocyte culture in vitro.
abstract: OBJECTIVE
  Large-scale expansion of epidermal keratinocytes is essential in the application of these cells for severe burn treatment in patients. Therefore, this study was designed to evaluate various conditions in the expansion of human epidermal keratinocytes.
  
  
  METHODS
  The epidermis was separated from the dermis of skin samples using dispase. The epidermis was trypsinized for keratinocyte isolation. Keratinocytes were cultured in various conditions, with or without a human dermal fibroblast feeder layer, mitomycin C treatment, and different culture media.
  
  
  RESULTS
  Our results suggest that keratinocytes cultured on a human dermal fibroblast feeder layer were grown for several passages. Extensive deformation and rapid deterioration were observed in the cultured cells without a feeder layer and in serum-free medium.
  
  
  CONCLUSIONS
  Human dermal fibroblasts treated with mitomycin C can provide optimal conditions for proliferation of keratinocytes.
authors.affiliation: From the Shiraz Transplant Research Center, Shiraz, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Kaviani
authors.forename: Bita
authors.initials: B
authors.lastname: Geramizadeh
authors.forename: Marjan
authors.initials: M
authors.lastname: Rahsaz
authors.forename: Saeed
authors.initials: S
authors.lastname: Marzban
mesh_terms: D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects; D002469:Cell Separation / Q000379:methods; D048430:Cell Shape; D002470:Cell Survival; D002478:Cells, Cultured; D018920:Coculture Techniques; D003470:Culture Media / Q000378:metabolism; D061252:Feeder Cells; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D016685:Mitomycin / Q000494:pharmacology; D061251:Primary Cell Culture; D013997:Time Factors
chemical_list: D003470:Culture Media; D016685:Mitomycin