title: Turning Squalene into Cationic Lipid Allows a Delivery of siRNA in Cultured Cells.
abstract: Covalent binding of squalene to siRNA has already been shown to be an interesting way of delivering siRNA in vivo. Whether squalene derivatives could also be used to deliver siRNA in cells without covalent binding similar to usual transfection with cationic lipids is the question addressed in this article. Accordingly, we investigated the activity of two squalene derivatives bearing a quaternary ammonium head group and a guanidinium group, respectively. The second derivative displayed interesting properties for delivering siRNA into cells in vitro.
authors.affiliation: 1Université Paris-Sud, Laboratoire de Vectorologie et Thérapeutiques Anticancéreuses, UMR 8203, Villejuif, France.|2Centre National de la Recherche Scientifique (CNRS), Laboratoire de Vectorologie et Thérapeutiques Anticancéreuses, UMR 8203, Villejuif, France.|3Gustave Roussy Cancer Campus Grand Paris, Laboratoire de Vectorologie et Thérapeutiques Anticancéreuses, UMR 8203, Villejuif, France.
authors.forename: Jean-Rémi
authors.initials: JR
authors.lastname: Bertrand
authors.affiliation: 1Université Paris-Sud, Laboratoire de Vectorologie et Thérapeutiques Anticancéreuses, UMR 8203, Villejuif, France.|2Centre National de la Recherche Scientifique (CNRS), Laboratoire de Vectorologie et Thérapeutiques Anticancéreuses, UMR 8203, Villejuif, France.|3Gustave Roussy Cancer Campus Grand Paris, Laboratoire de Vectorologie et Thérapeutiques Anticancéreuses, UMR 8203, Villejuif, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Lucas
authors.affiliation: 1Université Paris-Sud, Laboratoire de Vectorologie et Thérapeutiques Anticancéreuses, UMR 8203, Villejuif, France.|2Centre National de la Recherche Scientifique (CNRS), Laboratoire de Vectorologie et Thérapeutiques Anticancéreuses, UMR 8203, Villejuif, France.|3Gustave Roussy Cancer Campus Grand Paris, Laboratoire de Vectorologie et Thérapeutiques Anticancéreuses, UMR 8203, Villejuif, France.
authors.forename: Ngoc Minh
authors.initials: NM
authors.lastname: Pham
authors.affiliation: 4Centre National de la Recherche Scientifique (CNRS), UMR 8126, Villejuif, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Durieu
authors.affiliation: 5Université Paris-Sud, Institut Galien, CNRS UMR 8612, Châtenay-Malabry, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Couvreur
authors.affiliation: 1Université Paris-Sud, Laboratoire de Vectorologie et Thérapeutiques Anticancéreuses, UMR 8203, Villejuif, France.|2Centre National de la Recherche Scientifique (CNRS), Laboratoire de Vectorologie et Thérapeutiques Anticancéreuses, UMR 8203, Villejuif, France.|3Gustave Roussy Cancer Campus Grand Paris, Laboratoire de Vectorologie et Thérapeutiques Anticancéreuses, UMR 8203, Villejuif, France.
authors.forename: Claude Paul
authors.initials: CP
authors.lastname: Malvy
authors.affiliation: 5Université Paris-Sud, Institut Galien, CNRS UMR 8612, Châtenay-Malabry, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Desmaële
mesh_terms: D002412:Cations; D045744:Cell Line, Tumor; D006801:Humans; D052578:Ionic Liquids / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000008:administration & dosage*; D013185:Squalene / Q000378:metabolism*
chemical_list: D002412:Cations; D052578:Ionic Liquids; D034741:RNA, Small Interfering; D013185:Squalene