title: Generation and Characterization of a New Monoclonal Antibody Against CXCL4.
abstract: CXCL4 plays important roles in numerous disease processes, which makes the CXCL4 signaling pathway a potential therapeutic target. In this study, we aimed to develop a neutralizing antibody against both human and mouse CXCL4. Rats were immunized with recombinant human CXCL4 (rhCXCL4). Hybridoma clones were created by fusion of the immunized rat spleen cells with mouse myeloma SP2/0 cells and screened using recombinant mouse CXCL4 (rmCXCL4) and rhCXCL4. The CXCL4 monoclonal antibody (CXCL4 MAb) produced by the 16D6-3 hybridoma clone was sequenced and characterized by Western blot and Biacore assays. It recognized both human and mouse CXCL4 with high affinity and neutralized the effect of rhCXCL4 in vitro. Thus, the antibody may be used in the studies of CXCL4 in murine disease models and as a template in the antibody humanization for clinical developments.
authors.affiliation: 1 Laboratory of Regeneromics, School of Pharmacy, School of Agriculture and Biology, Shanghai Jiao Tong University , Shanghai, China .
authors.forename: Jing
authors.initials: J
authors.lastname: Gao
authors.forename: Mingyuan
authors.initials: M
authors.lastname: Wu
authors.forename: Jin
authors.initials: J
authors.lastname: Gao
authors.forename: Xia
authors.initials: X
authors.lastname: Wang
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Shunying
authors.initials: S
authors.lastname: Zhu
authors.forename: Yan
authors.initials: Y
authors.lastname: Yu
authors.forename: Wei
authors.initials: W
authors.lastname: Han
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000096:biosynthesis / Q000737:chemistry*; D045744:Cell Line, Tumor; D006801:Humans; D006825:Hybridomas; D008297:Male; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008969:Molecular Sequence Data; D010978:Platelet Factor 4 / Q000276:immunology*; D017207:Rats, Sprague-Dawley; D061905:Single-Domain Antibodies / Q000737:chemistry
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D061905:Single-Domain Antibodies; D010978:Platelet Factor 4