title: Heme precursor injection is effective for Arthromyces ramosus peroxidase fusion protein production by a silkworm expression system.
abstract: Recombinant peroxidase from Arthromyces ramosus, fused with domains of antibody-binding proteins, was successfully obtained by a silkworm larvae expression system. The catalytic activity of the fusion peroxidase was increased 6-fold with the injection of 5-aminolevulinic acid into silkworm larvae as a heme precursor.
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Fukuoka 819-0395, Japan; Hitachi Aloka Medical, Ltd., 6-22-1 Mure, Mitaka-shi, Tokyo 181-8622, Japan.
authors.forename: Kounosuke
authors.initials: K
authors.lastname: Hayashi
authors.affiliation: Laboratory of Silkworm Science, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan.
authors.forename: Jae Man
authors.initials: JM
authors.lastname: Lee
authors.affiliation: Hitachi Aloka Medical, Ltd., 6-22-1 Mure, Mitaka-shi, Tokyo 181-8622, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Tomozoe
authors.affiliation: Laboratory of Silkworm Science, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kusakabe
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Fukuoka 819-0395, Japan; Center for Future Chemistry, Kyushu University, 744 Motooka, Fukuoka 819-0395, Japan. Electronic address: nori_kamiya@mail.cstm.kyushu-u.ac.jp.
authors.forename: Noriho
authors.initials: N
authors.lastname: Kamiya
mesh_terms: D000363:Agaricales / Q000201:enzymology*; D000622:Aminolevulinic Acid / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D055162:Biocatalysis; D012831:Bombyx / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D015870:Gene Expression; D006418:Heme / Q000378:metabolism*; D007814:Larva / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D010544:Peroxidases / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism
chemical_list: D011993:Recombinant Fusion Proteins; D006418:Heme; D000622:Aminolevulinic Acid; D010544:Peroxidases
keywords: 5-Aminolevulinic acid; Baculovirus expression system; Heme protein; Peroxidase; Silkworm larvae