title: H5 avian influenza virus pathotyping using oligonucleotide microarray.
abstract: The H5 avian influenza virus subtype has huge impact on the poultry industry. Rapid diagnosis and accurate identification of the highly pathogenic avian influenza virus and low-pathogenicity avian influenza virus is essential, especially during H5 outbreaks and surveillance. To this end, a novel and rapid strategy for H5 virus molecular pathotyping is presented. The specific hemagglutinin gene of the H5 virus and the basic amino acid number of the motif at the hemagglutinin precursor protein cleavage site were detected using oligonucleotide microarray. Highly pathogenic and low-pathogenicity avian influenza viruses in Taiwan were differentiated using 13 microarray probes with the naked eye. The detection limit reached 3.4 viral RNA copies, 1000 times more sensitive than reverse transcription polymerase chain reaction. Thus, the oligonucleotide microarray would provide an alternative H5 pathogenicity determination using the naked eye for laboratories lacking facilities.
authors.affiliation: School of Veterinary Medicine, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, Taiwan. Electronic address: lcwang@ntu.edu.tw.
authors.forename: Lih-Chiann
authors.initials: LC
authors.lastname: Wang
authors.affiliation: School of Veterinary Medicine, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, Taiwan. Electronic address: huangchienhao@hotmail.com.
authors.forename: Dean
authors.initials: D
authors.lastname: Huang
authors.affiliation: Animal Health Research Institute, Council of Agriculture, Taipei, Taiwan. Electronic address: mccheng@mail.nvri.gov.tw.
authors.forename: Ming-Chu
authors.initials: MC
authors.lastname: Cheng
authors.affiliation: Animal Health Research Institute, Council of Agriculture, Taipei, Taiwan. Electronic address: shlee@mail.nvri.gov.tw.
authors.forename: Shu-Hwae
authors.initials: SH
authors.lastname: Lee
authors.affiliation: School of Veterinary Medicine, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, Taiwan. Electronic address: chingho@ntu.edu.tw.
authors.forename: Ching-Ho
authors.initials: CH
authors.lastname: Wang
mesh_terms: D000818:Animals; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics*; D009980:Influenza A virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D005585:Influenza in Birds / Q000821:virology*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011200:Poultry; D012680:Sensitivity and Specificity; D013624:Taiwan
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; C109963:hemagglutinin, avian influenza A virus
keywords: Avian influenza virus; Oligonucleotide microarray; Pathotyping