title: Hollow chitosan/alginate nanocapsules for bioactive compound delivery.
abstract: This work aimed at the development of biodegradable nanocapsules as carriers of two bioactive compounds, 5-aminosalycilic acid and glycomacropeptide. Nanocapsules were produced through layer-by-layer (LbL) deposition of chitosan (CH) and alginate (ALG) layers on polystyrene nanoparticles. The bioactive compounds were incorporated on the third layer of the nanocapsules being its encapsulation efficiency and release behaviour evaluated. The LbL deposition process, stability, morphology and size of the multilayer nanocapsules were monitored by means of zeta potential and transmission electron microscopy (TEM). The bioactive compounds release from the CH/ALG nanocapsules was successfully described by a mathematical model (linear superimposition model - LSM), which allowed concluding that bioactive compounds release is due to both Brownian motion and the polymer relaxation of the CH/ALG layers. Final results demonstrated that the synthesized LbL hollow nanocapsules presented spherical morphology and a good capacity to encapsulate different bioactive compounds, being the best results obtained for the system containing 5-aminosalycilic acid (with an encapsulation efficiency of approximately 70%). CH/ALG multilayer nanocapsules could be a promising carrier of bioactive compounds for applications in food and pharmaceutical industries.
authors.affiliation: Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: Melissa C
authors.initials: MC
authors.lastname: Rivera
authors.affiliation: Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: Ana C
authors.initials: AC
authors.lastname: Pinheiro
authors.affiliation: Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: Ana I
authors.initials: AI
authors.lastname: Bourbon
authors.affiliation: Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Cerqueira
authors.affiliation: Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal. Electronic address: avicente@deb.uminho.pt.
authors.forename: António A
authors.initials: AA
authors.lastname: Vicente
mesh_terms: D000464:Alginates / Q000737:chemistry*; D002364:Caseins / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D004337:Drug Carriers; D004339:Drug Compounding; D065546:Drug Liberation; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D007700:Kinetics; D016014:Linear Models; D019804:Mesalamine / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D053769:Nanocapsules / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D010446:Peptide Fragments / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*
chemical_list: D000464:Alginates; D002364:Caseins; D004337:Drug Carriers; D006603:Hexuronic Acids; D053769:Nanocapsules; D010446:Peptide Fragments; D011137:Polystyrenes; C065989:caseinomacropeptide; D019804:Mesalamine; D020723:Glucuronic Acid; D048271:Chitosan
keywords: Alginate; Biodegradable polysaccharides; Chitosan; Functional compounds; Multilayer