title: Primary chicken embryo fibroblasts seeded acellular dermal matrix (3-D ADM) improve regeneration of full thickness skin wounds in rats.
abstract: Rat skins were deepithelialized and decellularized by hypertonic saline and sodium deoxycholate (SDC), respectively. Primary chicken embryo fibroblasts (P-CEF) were cultured and seeded on prepared acellular dermal matrix (ADM). A full thickness skin defect (20×20 mm(2)) was created in thirty-six rats and randomly divided into three equal groups. Defect was left open, repaired with ADM and ADM seeded with P-CEF (3-D ADM) in groups 1, 2 and 3, respectively. By day 28, the treated wounds healed completely without scar. By day 7 hydroxyproline contents was higher in group 3 as compared to groups 1 and 2. There was slightly more B cell response in animals implanted with ADM and 3-D ADM. At day 21, stimulation index was lower with acellular dermis antigen as compared to 3-D ADM antigen. In group 1 on day 3, the granulation tissue showed more inflammatory reaction, fibroplasia and neovascularization as compared to group 2 and 3. By day 28, there was complete epithelization was observed in all groups over. However, a large scar was observed in group 1. The graft was completely absorbed and replaced with densely thick and best arranged collagen fibers. On day 7, malonyldialdehyde and superoxide dismutase levels were significantly (P<0.05) increased in group 1. Reduced glutathione values increased and reached to near normal in groups 2 and 3. Catalase values were significantly (P<0.05) higher in group 1 at different time intervals. SEM samples of group 2 showed ingrowth of fibroblasts into acellular matrix at host graft junction. However, in group 3 fibroblasts were infiltrated within the pores of graft. It was concluded that P-CEF cells seeded ADM facilitated early and better healing.
authors.affiliation: Division of Surgery, Indian Veterinary Research Institute, Izatnagar 243122, Uttar Pradesh, India; Department of Veterinary Surgery and Radiology, College of Veterinary Science and Animal Husbandry, Narendra Deva University of Agriculture and Technology, Kumarganj, Faizabad 224229, Uttar Pradesh, India. Electronic address: dranilvet2007@gmail.com.
authors.forename: Anil Kumar
authors.initials: AK
authors.lastname: Gangwar
authors.affiliation: Division of Surgery, Indian Veterinary Research Institute, Izatnagar 243122, Uttar Pradesh, India. Electronic address: naveen.ivri1961@gmail.com.
authors.forename: Naveen
authors.initials: N
authors.lastname: Kumar
authors.affiliation: Department of Veterinary Surgery and Radiology, College of Veterinary Science and Animal Husbandry, Narendra Deva University of Agriculture and Technology, Kumarganj, Faizabad 224229, Uttar Pradesh, India. Electronic address: drsangeetavet2007@rediffmail.com.
authors.forename: Sangeeta Devi
authors.initials: SD
authors.lastname: Khangembam
authors.affiliation: Division of Surgery, Indian Veterinary Research Institute, Izatnagar 243122, Uttar Pradesh, India; Department of Veterinary Surgery and Radiology, College of Veterinary Science and Animal Husbandry, Junagadh Agricultural University, Junagadh 362001, Gujarat, India. Electronic address: bharadwaj374@gmail.com.
authors.forename: Vineet
authors.initials: V
authors.lastname: Kumar
authors.affiliation: Division of Pathology, Indian Veterinary Research Institute, Izatnagar 243122, Uttar Pradesh, India. Electronic address: rajendra_singh5747@rediffmail.com.
authors.forename: Rajendra
authors.initials: R
authors.lastname: Singh
mesh_terms: D062088:Acellular Dermis; D000818:Animals; D064987:Cell- and Tissue-Based Therapy; D002642:Chick Embryo; D005109:Extracellular Matrix; D005347:Fibroblasts / Q000637:transplantation*; D051381:Rats; D012038:Regeneration; D012867:Skin / Q000254:growth & development / Q000473:pathology; D014945:Wound Healing
keywords: 3-D ADM; Oxidative stress; Primary chicken embryo fibroblasts; Rats; Skin wound healing