title: [The influence of recombinant nucleophosmin 1 on artificial RNA internalization into human adenocarcinoma MCF-7 cells].
abstract: In this study we obtained and characterized the recombinant analogue of multifunctional nucleolar phosphoprotein nucleophosmin 1 (NPM1) involved in crucial cellular processes such as transcription, reparation and mitosis. The influence ofnucleophosmin 1 on extrcellular RNAs accumulation in human adenocarcinoma cells MCF-7 was analyzed. It was found that incubation of AluY RNA (n > 300 nt), U24 snoRNA analogues (n ~ 80 nt) with Npm1-His6 resulted in RNA-protein non-covalent complexes formation, but not in case of the short oligoribonucleotide (n = 22 nt). It was shown that interaction of AluY RNA analogue with Npm1-His6 significantly increases transfection efficacy of the RNA into MCF-7 human cells. Altogether, these data allow us to conclude, that nucleophosmin 1 not only binds RNA with complex secondary structure, but also promotes uptake and internalization of such RNA by human cells.
authors.forename: A V
authors.initials: AV
authors.lastname: Savelyeva
authors.forename: D V
authors.initials: DV
authors.lastname: Semenov
authors.forename: G A
authors.initials: GA
authors.lastname: Stepanov
authors.forename: D N
authors.initials: DN
authors.lastname: Baryakin
authors.forename: E V
authors.initials: EV
authors.lastname: Kuligina
authors.forename: I V
authors.initials: IV
authors.lastname: Rabinov
authors.forename: O A
authors.initials: OA
authors.lastname: Koval
authors.forename: V A
authors.initials: VA
authors.lastname: Richter
mesh_terms: D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D061986:MCF-7 Cells / Q000187:drug effects / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D000090243:Nucleophosmin; D015202:Protein Engineering / Q000379:methods; D012313:RNA / Q000737:chemistry / Q000378:metabolism* / Q000493:pharmacokinetics; D020537:RNA, Small Nucleolar / Q000493:pharmacokinetics; D011994:Recombinant Proteins / Q000235:genetics / Q000494:pharmacology; D014162:Transfection / Q000379:methods*
chemical_list: C000716967:NPM1 protein, human; D009687:Nuclear Proteins; D020537:RNA, Small Nucleolar; D011994:Recombinant Proteins; D000090243:Nucleophosmin; D012313:RNA