title: Interaction between mesenchymal stem cells and endothelial cells restores endothelial permeability via paracrine hepatocyte growth factor in vitro.
abstract: BACKGROUND
  Mesenchymal stem cells (MSCs) have potent stabilising effects on vascular endothelium injury, inhibiting endothelial permeability in lung injury via paracrine hepatocyte growth factor (HGF). Recently, it has been indicated that MSCs secrete more factors by MSC-endothelial cell (MSC-EC) interactions. We hypothesised that MSC-EC interactions restore endothelial permeability induced by lipopolysaccharide (LPS) via paracrine HGF.
  
  
  METHODS
  We investigated the endothelial permeability induced by LPS under two co-culture conditions. Human pulmonary microvascular endothelial cells (HPMECs) were added into the upper chambers of cell-culture inserts, while two different co-culture conditions were used in the lower side of the transwells, as follows: (1) MSC-EC interaction group: MSCs and HPMECs contact co-culture; (2) MSC group: MSCs only. The endothelial paracellular and transcellular permeabilities in the upper side of transwells were detected. Then the concentration of HGF was measured in the culture medium by using an enzyme-linked immunosorbent assay kit, followed by neutralisation of HGF with anti-HGF antibody in the co-culture medium. In addition, adherens junction and cytoskeleton protein expressions were measured by Western blot and immunofluorescence. HPMEC proliferation was analysed by bromodeoxyuridine incorporation assay.
  
  
  RESULTS
  The paracellular permeability significantly increased after LPS stimulation in a dose-dependent and time-dependent manner. Meanwhile, MSC-EC interaction more significantly decreased endothelial paracellular and transcellular permeability induced by LPS. Moreover, HGF levels in the MSC-EC interaction group were much higher than those of the MSC group. However, neutralising HGF with anti-HGF antibody inhibited the role of MSC-EC interaction in improving endothelial permeability. Compared with the MSC group, MSC-EC interaction increased vascular endothelial (VE)-cadherin and occludin protein expression, reduced caveolin-1 protein expression in HPMECs, and restored remodelling of F-actin and junctional localisation of VE-cadherin. Furthermore, the proliferation ratio in the MSC-EC interaction group was higher than that of the MSC group. However, the effects of MSCs were significantly blocked by anti-HGF antibody.
  
  
  CONCLUSIONS
  These data suggested that MSC-EC interaction decreased endothelial permeability induced by LPS, which was attributed mainly to HGF secreted by MSCs. The main mechanisms by which HGF restored the integrity of endothelial monolayers were remodelling of endothelial intercellular junctions, decreasing caveolin-1 protein expression, and inducing proliferation in HPMECs.
authors.affiliation: Department of Critical Care Medicine, Zhong-Da Hospital, School of Medicine, Southeast University, #87 Dingjiaqiao Road, Nanjing, 210009, Jiangsu, P.R. China. chenqihong00@163.com.
authors.forename: Qi-Hong
authors.initials: QH
authors.lastname: Chen
authors.affiliation: Department of Critical Care Medicine, Zhong-Da Hospital, School of Medicine, Southeast University, #87 Dingjiaqiao Road, Nanjing, 210009, Jiangsu, P.R. China. airanliu2013@gmail.com.
authors.forename: Ai-Ran
authors.initials: AR
authors.lastname: Liu
authors.affiliation: Department of Critical Care Medicine, Zhong-Da Hospital, School of Medicine, Southeast University, #87 Dingjiaqiao Road, Nanjing, 210009, Jiangsu, P.R. China. haiboq2000@gmail.com.
authors.forename: Hai-Bo
authors.initials: HB
authors.lastname: Qiu
authors.affiliation: Department of Critical Care Medicine, Zhong-Da Hospital, School of Medicine, Southeast University, #87 Dingjiaqiao Road, Nanjing, 210009, Jiangsu, P.R. China. yiyiyang2004@gmail.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
mesh_terms: D000199:Actins / Q000378:metabolism; D015703:Antigens, CD / Q000378:metabolism; D015820:Cadherins / Q000378:metabolism; D051242:Caveolin 1 / Q000378:metabolism; D018929:Cell Culture Techniques; D019070:Cell Lineage; D002478:Cells, Cultured; D018920:Coculture Techniques; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D004730:Endothelium, Vascular / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D017228:Hepatocyte Growth Factor / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D062793:Occludin / Q000378:metabolism; D019899:Paracrine Communication; D010539:Permeability / Q000187:drug effects; D000099152:Cadherin 5
chemical_list: D000199:Actins; D015703:Antigens, CD; D015820:Cadherins; D051242:Caveolin 1; D017228:Hepatocyte Growth Factor; D008070:Lipopolysaccharides; D062793:Occludin; D000099152:Cadherin 5; C066855:HGF protein, human