title: Optimization, purification and characterization of pectinases from pectinolytic strain, Aspergillus foetidus MTCC 10559.
abstract: A strain (MPUAT-2), isolated from coconut hull and identified as Aspergillus foetidus MTCC 10559, was used for pectinase production. Optimum pectinase production was obtained at pH 8.0 and temperature 35 degrees C under static conditions in submerged fermentation after 5 days of incubation. Orange peel, a byproduct of fruit industry, was used as a sole carbon source (3% w/v) to produce high pectinase, thus making the process cost effective. The culture filtrate was analyzed for pectin methyl esterase (PME) and endopolygalacturonase (endo-PG) enzymes. The enzymes, PME and endo-PG were purified using ammonium sulphate precipitation and molecular exclusion chromatography (Sephadex G-75) with corresponding recovery of 39.3 and 44.3%. The partially purified enzymes were also characterized for their kinetic properties.
authors.forename: Sunil
authors.initials: S
authors.lastname: Kumar
authors.forename: Narender K
authors.initials: NK
authors.lastname: Jain
authors.forename: Kailash C
authors.initials: KC
authors.lastname: Sharma
authors.forename: Ranjeet
authors.initials: R
authors.lastname: Paswan
authors.forename: Brijesh K
authors.initials: BK
authors.lastname: Mishra
authors.forename: Ramakrishnan
authors.initials: R
authors.lastname: Srinivasan
authors.forename: Shiwani
authors.initials: S
authors.lastname: Mandhania
mesh_terms: D001230:Aspergillus / Q000145:classification / Q000201:enzymology*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D015966:Gene Expression Regulation, Fungal / Q000502:physiology; D011096:Polygalacturonase / Q000145:classification / Q000235:genetics / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D011096:Polygalacturonase