title: Novel microcatheter-based intracarotid delivery approach for MCAO/R mice.
abstract: The intra-arterial (IA) model by microcatheter administration was an effective way to deliver drugs or cells to the brain. All of these models were carried out introduced in rat rather than mice for the difficult and technically challenging due to their small caliber. In 2014, Alejandro Santillan first introduced this model in mice and we found that most of the operational steps were similar with the middle cerebral artery occlusion and reperfusion (MCAO/R) model. We attempted to combine these two techniques into a single model in mice and discovered that this technique was indeed possible. In our work, 12C57Bl/6J male mice were carried on middle cerebral artery occlusion for 60min and then the intra-arterial microcatheter was placed into the internal carotid artery (ICA) from the external carotid artery (ECA). GFP-Luc-Pro labeled mNSCs were infused through the microcatheter and then the blood flow perfusion was reestablished subsequently. The results showed that all 12 mice were carried on successfully the model of middle cerebral artery occlusion, and the placement of the microcatheter and the mNSCs perfusion were completed smoothly without exception. Which means that it is logical to combine the two models into one in order to facilitate studying of stroke. Meanwhile, during the dissection, we found the variation of occipital artery (OA) was noticeable and we classified first time this variation into four categories to attempt to protect the OA.
authors.affiliation: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan City, Hubei Province, 430060, China; Department of Neurosurgery, Yale University, 333 Cedar Street, FMB 410, New Haven, CT 06520, USA.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Cai
authors.affiliation: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan City, Hubei Province, 430060, China.
authors.forename: Zhibiao
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Neurosurgery, Yale University, 333 Cedar Street, FMB 410, New Haven, CT 06520, USA.
authors.forename: Derek Kai
authors.initials: DK
authors.lastname: Kong
authors.affiliation: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan City, Hubei Province, 430060, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan City, Hubei Province, 430060, China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan City, Hubei Province, 430060, China.
authors.forename: Baohui
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan City, Hubei Province, 430060, China. Electronic address: chenqx666@sohu.com.
authors.forename: Qianxue
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Department of Neurosurgery, Yale University, 333 Cedar Street, FMB 410, New Haven, CT 06520, USA.
authors.forename: Jiangbing
authors.initials: J
authors.lastname: Zhou
mesh_terms: D000818:Animals; D002342:Carotid Artery, External / Q000033:anatomy & histology; D002343:Carotid Artery, Internal / Q000033:anatomy & histology; D002408:Catheters, Indwelling; D020244:Infarction, Middle Cerebral Artery / Q000628:therapy*; D008297:Male; D008810:Mice, Inbred C57BL; D058953:Neural Stem Cells / Q000637:transplantation*; D015424:Reperfusion
keywords: Animal model; Carotid artery; Mice; Middle cerebral artery occlusion