title: Low fouling label-free DNA sensor based on polyethylene glycols decorated with gold nanoparticles for the detection of breast cancer biomarkers.
abstract: A label-free and low fouling biosensor based on functional polyethylene glycols selective for breast cancer susceptibility gene (BRCA1) is reported. Sensory interfaces were prepared through the modification of a glassy carbon electrode with highly cross-linked polyethylene glycol (PEG) film containing amine groups, followed by the self-assembly of gold nanoparticles and the immobilization of BRCA1 complementary single-strand 19-mer oligonucleotides. In the presence of a specific BRCA1 sequence capture and hybridization results in interfacial change sensitively monitored using electrochemical impedance spectroscopy. The combined utilization of a PEG polymer film and gold nanoparticle mixed interface enables very high levels of sensitivity and a highly effective assaying in patient samples. Assay linear range was from 50.0 fM to 1.0 nM, with a limit of detection of 1.72 fM. Furthermore, this label-free DNA sensor has been used for assaying BRCA1 in serum samples, showing its feasible potential for diagnostic applications in clinical analysis of breast cancer gene BRCA1. Foreseeable, this sensor made on this basis undoubtedly provide the most effective and sensitive detection for BRCA1.
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
authors.forename: Wenting
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Breast Surgery, The Eighth Peoples' Hospital of Qingdao, Qingdao 266100, China.
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Fan
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
authors.forename: Shenghao
authors.initials: S
authors.lastname: Xu
authors.affiliation: Department of Chemistry, University of Oxford, Oxford OX1 3QZ, UK. Electronic address: jason.davis@chem.ox.ac.uk.
authors.forename: Jason J
authors.initials: JJ
authors.lastname: Davis
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China. Electronic address: xiliangluo@hotmail.com.
authors.forename: Xiliang
authors.initials: X
authors.lastname: Luo
mesh_terms: D019313:BRCA1 Protein / Q000097:blood / Q000235:genetics; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000032:analysis / Q000097:blood / Q000235:genetics; D015374:Biosensing Techniques / Q000379:methods; D001940:Breast / Q000378:metabolism / Q000473:pathology; D001943:Breast Neoplasms / Q000097:blood / Q000175:diagnosis / Q000235:genetics*; D004247:DNA / Q000097:blood / Q000235:genetics*; D055664:Electrochemical Techniques / Q000379:methods; D004566:Electrodes; D005260:Female; D019398:Genes, BRCA1; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009693:Nucleic Acid Hybridization / Q000379:methods; D011092:Polyethylene Glycols / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D019313:BRCA1 Protein; D014408:Biomarkers, Tumor; D011092:Polyethylene Glycols; D006046:Gold; D004247:DNA
keywords: BRCA1; Cancer biomarker; Gold nanoparticles; Label-free; Polyethylene glycols