title: Ex vivo aorta patch model for analysis of cellular adhesion.
abstract: The vascular endothelium as well as subendothelium are objects of many researches as it is directly involved in a multiplicity of physiological and pathological settings. Detailed study of endothelial function became feasible with the development of techniques to culture endothelial cells (EC) in vitro. Limitations of this approach have become apparent with the realization that cell culture dedifferentiate with time and do not exhibit properties of intact tissue. Here we describe the development of a novel ex vivo tissue model to study cell-vascular wall interactions by using isolated mouse aorta patches. Validation of this model was performed by demonstrating cell attachment and changes in cell shape typical for cell spreading during adhesion. A major advantage of this model is that cell-endothelium interaction and its molecular backgrounds can now be studied more feasibly on an intact and native tissue.
authors.affiliation: Department of General, Visceral and Thoracic Surgery, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, D-20246 Hamburg, Germany; Department of Biochemistry and Signal Transduction, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, D-20246 Hamburg, Germany. Electronic address: y.lin@uke.de.
authors.forename: Yuan-Na
authors.initials: YN
authors.lastname: Lin
authors.affiliation: Department of Biochemistry and Signal Transduction, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, D-20246 Hamburg, Germany. Electronic address: chandichada@gmail.com.
authors.forename: Raymond Nqobizitha
authors.initials: RN
authors.lastname: Thata
authors.affiliation: Microscopy Imaging Facility, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, D-20246 Hamburg, Germany. Electronic address: a.failla@uke.de.
authors.forename: Antonio Virgilio
authors.initials: AV
authors.lastname: Failla
authors.affiliation: Department of Vascular Medicine, University Heart Center, Hamburg-Eppendorf, Martinistrasse 52, D-20246 Hamburg, Germany. Electronic address: m.geissen@uke.de.
authors.forename: Markus
authors.initials: M
authors.lastname: Geissen
authors.affiliation: Department of Vascular Medicine, University Heart Center, Hamburg-Eppendorf, Martinistrasse 52, D-20246 Hamburg, Germany. Electronic address: g.daum@uke.de.
authors.forename: Guenter
authors.initials: G
authors.lastname: Daum
authors.affiliation: Department of Biochemistry and Signal Transduction, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, D-20246 Hamburg, Germany. Electronic address: s.windhorst@uke.de.
authors.forename: Sabine
authors.initials: S
authors.lastname: Windhorst
mesh_terms: D000818:Animals; D001011:Aorta / Q000166:cytology / Q000502:physiology*; D002448:Cell Adhesion / Q000502:physiology*; D002450:Cell Communication / Q000502:physiology; D018929:Cell Culture Techniques; D054337:Cell Dedifferentiation / Q000502:physiology*; D048430:Cell Shape / Q000502:physiology; D004730:Endothelium, Vascular / Q000166:cytology* / Q000502:physiology; D051379:Mice
keywords: Aorta; Cell adhesion; Endothelial cells; Vascular model