title: Hematologic and biochemical reference intervals for Wild Boar (Sus scrofa) captured by cage trap.
abstract: BACKGROUND
  Establishing reference intervals (RI) for hematologic and biochemical variables in wild animals presents great challenges because capture stress or anesthesia during sampling can affect blood variables.
  
  
  OBJECTIVE
  The aims of this study were to establish RI for hematologic and blood biochemistry variables for Wild Boar (Sus scrofa) caught using cage traps, and provide information on the studied variables for different age groups.
  
  
  METHODS
  Blood samples were obtained from 89 Wild Boars captured by cage trap between 2005 and 2013 in northeastern Spain. Piglets were handled without anesthesia, while juvenile and adult animals were anesthetized using a combination of tiletamine and zolazepam. Blood samples were collected from the anterior vena cava and were placed into plain and EDTA tubes. Thirteen hematologic and 21 biochemical variables were determined. Reference intervals for piglets and juvenile and adult groups were determined, and differences between these groups were statistically analyzed.
  
  
  RESULTS
  Adults had higher HGB, PCV, MCH, MCHC, neutrophil count, and total protein, albumin, creatinine, and chloride concentrations than juveniles; in contrast, juveniles had higher values for lymphocyte count, cholesterol concentration, and ALP activity.
  
  
  CONCLUSIONS
  Reference intervals determined in this study provide a baseline for interpreting hematologic and biochemical results in Wild Boar at different age stages, and contribute to optimization of the management of this species.
authors.affiliation: Servei d'Ecopatologia de Fauna Salvatge (SEFaS) - Wildlife Health Research Group, Departament de Medicina i Cirurgia Animals, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
authors.forename: Encarna
authors.initials: E
authors.lastname: Casas-Díaz
authors.affiliation: Servei d'Ecopatologia de Fauna Salvatge (SEFaS) - Wildlife Health Research Group, Departament de Medicina i Cirurgia Animals, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
authors.forename: Francesc
authors.initials: F
authors.lastname: Closa-Sebastià
authors.affiliation: Servei d'Ecopatologia de Fauna Salvatge (SEFaS) - Wildlife Health Research Group, Departament de Medicina i Cirurgia Animals, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
authors.forename: Ignasi
authors.initials: I
authors.lastname: Marco
authors.affiliation: Servei d'Ecopatologia de Fauna Salvatge (SEFaS) - Wildlife Health Research Group, Departament de Medicina i Cirurgia Animals, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
authors.forename: Santiago
authors.initials: S
authors.lastname: Lavín
authors.affiliation: Servei d'Hematologia Clínica Veterinària (SHCV) - Veterinary Clinical Hematology Service, Departament de Medicina i Cirurgia Animals, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
authors.forename: Ester
authors.initials: E
authors.lastname: Bach-Raich
authors.affiliation: Servei d'Hematologia Clínica Veterinària (SHCV) - Veterinary Clinical Hematology Service, Departament de Medicina i Cirurgia Animals, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
authors.forename: Rafaela
authors.initials: R
authors.lastname: Cuenca
mesh_terms: D000375:Aging; D000818:Animals; D001772:Blood Cell Count / Q000662:veterinary*; D001774:Blood Chemical Analysis / Q000662:veterinary*; D005260:Female; D006403:Hematologic Tests / Q000662:veterinary; D008297:Male; D012016:Reference Values; D012149:Restraint, Physical; D034421:Sus scrofa / Q000097:blood*
keywords: Adults; anesthesia; juveniles; management; piglets; wildlife