title: Hypothermic temperature effects on organ survival and restoration.
abstract: A three-dimensional multicellular organism maintains the biological functions of life support by using the blood circulation to transport oxygen and nutrients and to regulate body temperature for intracellular enzymatic reactions. Donor organ transplantation using low-temperature storage is used as the fundamental treatment for dysfunctional organs. However, this approach has a serious problem in that donor organs maintain healthy conditions only during short-term storage. In this study, we developed a novel liver perfusion culture system based on biological metabolism that can maintain physiological functions, including albumin synthesis, bile secretion and urea production. This system also allows for the resurrection of a severely ischaemic liver. This study represents a significant advance for the development of an ex vivo organ perfusion system based on biological metabolism. It can be used not only to address donor organ shortages but also as the basis of future regenerative organ replacement therapy.
authors.affiliation: 1] Department of Biological Science and Technology, Graduate School of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba. 278-8510, JAPAN [2] Laboratory for Organ Regeneration, RIKEN Center for Developmental Biology, Kobe, Hyogo. 650-0047, JAPAN.
authors.forename: Jun
authors.initials: J
authors.lastname: Ishikawa
authors.affiliation: Research Institute for Science and Technology, Tokyo University of Science, Noda, Chiba. 278-8510, JAPAN.
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Oshima
authors.affiliation: Department of Biological Science and Technology, Graduate School of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba. 278-8510, JAPAN.
authors.forename: Fumitaka
authors.initials: F
authors.lastname: Iwasaki
authors.affiliation: Department of Biological Science and Technology, Graduate School of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba. 278-8510, JAPAN.
authors.forename: Ryoji
authors.initials: R
authors.lastname: Suzuki
authors.affiliation: Department of Biological Science and Technology, Graduate School of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba. 278-8510, JAPAN.
authors.forename: Joonhong
authors.initials: J
authors.lastname: Park
authors.affiliation: Research Institute for Science and Technology, Tokyo University of Science, Noda, Chiba. 278-8510, JAPAN.
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Nakao
authors.affiliation: Department of Biological Science and Technology, Graduate School of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba. 278-8510, JAPAN.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Matsuzawa-Adachi
authors.affiliation: Department of Biological Science and Technology, Graduate School of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba. 278-8510, JAPAN.
authors.forename: Taro
authors.initials: T
authors.lastname: Mizutsuki
authors.affiliation: Department of Biological Science and Technology, Graduate School of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba. 278-8510, JAPAN.
authors.forename: Ayaka
authors.initials: A
authors.lastname: Kobayashi
authors.affiliation: Department of Surgery, Keio University, School of Medicine, Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, JAPAN.
authors.forename: Yuta
authors.initials: Y
authors.lastname: Abe
authors.affiliation: 1] Department of Organ Fabrication, Keio University, School of Medicine, Shinanomachi, Shinjuku-ku, Tokyo 160-8582, JAPAN [2] Center for Development of Advanced Medical Technology, Jichi Medical University, Shimotsuke, Tochigi. 329-0431, JAPAN.
authors.forename: Eiji
authors.initials: E
authors.lastname: Kobayashi
authors.affiliation: 1] Laboratory for Organ Regeneration, RIKEN Center for Developmental Biology, Kobe, Hyogo. 650-0047, JAPAN [2] Organ Technologies Inc., Tokyo. 101-0048, JAPAN.
authors.forename: Katsunari
authors.initials: K
authors.lastname: Tezuka
authors.affiliation: 1] Laboratory for Organ Regeneration, RIKEN Center for Developmental Biology, Kobe, Hyogo. 650-0047, JAPAN [2] Research Institute for Science and Technology, Tokyo University of Science, Noda, Chiba. 278-8510, JAPAN [3] Organ Technologies Inc., Tokyo. 101-0048, JAPAN.
authors.forename: Takashi
authors.initials: T
authors.lastname: Tsuji
mesh_terms: D000818:Animals; D001646:Bile / Q000378:metabolism; D002478:Cells, Cultured; D019075:Electrophoresis, Capillary; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008115:Liver Regeneration; D016031:Liver Transplantation; D008297:Male; D055442:Metabolome; D051381:Rats; D017208:Rats, Wistar; D012709:Serum Albumin / Q000096:biosynthesis; D021241:Spectrometry, Mass, Electrospray Ionization; D013696:Temperature; D046509:Tissue Culture Techniques / Q000295:instrumentation / Q000379:methods*; D014022:Tissue Survival; D014508:Urea / Q000378:metabolism
chemical_list: D012709:Serum Albumin; D014508:Urea