title: Monoclonal antibodies against hepatitis B viral surface antigens and epitope grouping.
abstract: Monoclonal antibodies (MAbs) were generated against subtypes (ad/ad/rw) of the human hepatitis B viral surface antigen (HBsAg). Among dozens of antibodies that were generated, the majority was shown to commonly react with various ad/ay subtypes of the S protein. Epitope(s) of these antibodies were grouped by various immunoassay methods, and at least four distinct epitope regions were identified. Some of these antibodies were selected to formulate sandwich enzyme immunoassays for quantitative determinations of HBsAg in reconstituted specimens. Epitope-defined monoclonal antibodies with high affinity and specificity might be suitable for formulations as vaccines (containing a mixture of humanized monoclonal antibodies) for passive immunization in humans for immunoprophylaxis of HBV infection.
authors.affiliation: UBC Center for Reproductive Health , Vancouver, British Columbia, Canada .
authors.forename: Gregory
authors.initials: G
authors.lastname: Lee
authors.forename: Suefay
authors.initials: S
authors.lastname: Liu
mesh_terms: D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000737:chemistry*; D000914:Antibodies, Viral / Q000737:chemistry*; D018604:Epitope Mapping; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000737:chemistry / Q000276:immunology*; D006515:Hepatitis B virus / Q000276:immunology; D006801:Humans; D006825:Hybridomas; D051379:Mice; D011485:Protein Binding
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D000914:Antibodies, Viral; D000939:Epitopes; D006514:Hepatitis B Surface Antigens