title: Molecular characterization and phylogenetic analysis of transmissible gastroenteritis virus HX strain isolated from China.
abstract: BACKGROUND
  Porcine transmissible gastroenteritis virus (TGEV) is the major etiological agent of viral enteritis and severe diarrhea in suckling piglets. In China, TGEV has caused great economic losses, but its role in epidemic diarrhea is unclear. This study aims to reveal the etiological role of TGEV in piglet diarrhea via molecular characterization and phylogenetic analysis.
  
  
  RESULTS
  A TGEV-HX strain was isolated from China, and its complete genome was amplified, cloned, and sequenced. Sequence analysis indicated that it was conserved in the 5' and 3'-non-translated regions, and there were no insertions or deletions in nonstructural genes, such as ORF1a, ORF1b, ORF3a, ORF3b, and ORF7, as well as in genes encoding structural proteins, such as the envelope (E), membrane (M), and nucleoprotein (N) proteins. Furthermore, the phylogenetic analysis indicated that the TGEV-HX strain was more similar to the TGEV Purdue cluster than to the Miller cluster.
  
  
  CONCLUSIONS
  The present study described the isolation and genetic characterization of a TGEV-HX strain. The detailed analysis of the genetic variation of TGEVs in China provides essential information for further understanding the evolution of TGEVs.
authors.affiliation: College of Resources and Environment, Northeast Agricultural University, Harbin, 150030, People's Republic of China. liang679@163.com.|College of Life Sciences, Northeast Agricultural University, Harbin, Harbin, 150030, People's Republic of China. liang679@163.com.
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: Hu
authors.affiliation: College of Resources and Environment, Northeast Agricultural University, Harbin, 150030, People's Republic of China. 619483612@qq.com.
authors.forename: Nannan
authors.initials: N
authors.lastname: Li
authors.affiliation: College of Wildlife Resources, Northeast Forestry University, Harbin, 150040, People's Republic of China. beishugege@126.com.
authors.forename: Zhige
authors.initials: Z
authors.lastname: Tian
authors.affiliation: College of Life Sciences, Northeast Agricultural University, Harbin, Harbin, 150030, People's Republic of China. 158584843@qq.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Yin
authors.affiliation: National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150001, People's Republic of China. qld@hvri.ac.cn.
authors.forename: Liandong
authors.initials: L
authors.lastname: Qu
authors.affiliation: College of Resources and Environment, Northeast Agricultural University, Harbin, 150030, People's Republic of China. juanjuanqu@126.com.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Qu
mesh_terms: D000818:Animals; D001483:Base Sequence; D002681:China / Q000453:epidemiology; D005761:Gastroenteritis, Transmissible, of Swine / Q000453:epidemiology / Q000821:virology*; D016679:Genome, Viral / Q000235:genetics; D008854:Microscopy, Electron / Q000662:veterinary; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment / Q000662:veterinary; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013552:Swine / Q000821:virology; D005760:Transmissible gastroenteritis virus / Q000235:genetics* / Q000648:ultrastructure; D014759:Viral Envelope Proteins / Q000235:genetics; D017361:Viral Nonstructural Proteins / Q000235:genetics; D015678:Viral Structural Proteins / Q000235:genetics
chemical_list: D014759:Viral Envelope Proteins; D017361:Viral Nonstructural Proteins; D015678:Viral Structural Proteins