title: Characterization and bioactivities of a novel purified polysaccharide from Endothelium corneum gigeriae Galli.
abstract: In the present study, characterization and cardioprotective activities in vivo of a novel purified polysaccharide (PECGp) from Endothelium corneum gigeriae Galli were investigated. The results found that PECGp had a 96 kDa molecular weight. The backbone chains were composed of α-D-Glc and α-L-Rha linked by (1→4) glycosidic bonds. (1→4, 6) linked α-D-Glc and (1→2, 4) linked α-L-Rha in backbone chains with branches consisting of (1→4) linked α-D-Glc and terminal α-D-Glc-6-COOH. The assay of cardioprotective activities demonstrated that PECGp could significantly reduce the ST-segment elevation, prevented abnormal changes of heart muscle morphology, reverse abnormal hemodynamic and hemorheological parameters, rectify disorganized superoxide dismutase, nitric oxide synthase, nitric oxide, malondialdehyde, creatine kinase, creatine kinase MB fraction and lactate dehydrogenase levels. The results suggested that PECGp could be considered as a potential candidate for developing novel cardioprotective agents.
authors.affiliation: School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, Jiangsu, PR China; Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration, Huaiyin Institute of Technology, Huaian 223003, Jiangsu, PR China.
authors.forename: Qingping
authors.initials: Q
authors.lastname: Xiong
authors.affiliation: School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, Jiangsu, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Jing
authors.affiliation: Affiliated Huaian Hospital of Xuzhou Medical College, Huaian 223002, Jiangsu, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Medicine, Yunnan University, Kunming 650091, Yunnan, PR China.
authors.forename: Shangyong
authors.initials: S
authors.lastname: Zheng
authors.affiliation: School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, Jiangsu, PR China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, Jiangsu, PR China.
authors.forename: Songlin
authors.initials: S
authors.lastname: Li
authors.affiliation: School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, Jiangsu, PR China.
authors.forename: Qianghua
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Affiliated Huaian Hospital of Xuzhou Medical College, Huaian 223002, Jiangsu, PR China.
authors.forename: Youdong
authors.initials: Y
authors.lastname: Hu
authors.affiliation: School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, Jiangsu, PR China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Shi
authors.affiliation: School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, Jiangsu, PR China.
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, Jiangsu, PR China.
authors.forename: Dingyun
authors.initials: D
authors.lastname: Jiang
authors.affiliation: School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, Jiangsu, PR China; School of Medicine, Yunnan University, Kunming 650091, Yunnan, PR China. Electronic address: j1c2x3@gmail.com.
authors.forename: Changxing
authors.initials: C
authors.lastname: Jiang
mesh_terms: D000818:Animals; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D002316:Cardiotonic Agents / Q000737:chemistry* / Q000494:pharmacology*; D003402:Creatine Kinase / Q000097:blood / Q000378:metabolism; D052279:Creatine Kinase, MB Form / Q000097:blood; D004562:Electrocardiography / Q000187:drug effects; D006439:Hemodynamics / Q000187:drug effects; D018056:Hemorheology; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000097:blood / Q000378:metabolism; D009203:Myocardial Infarction / Q000097:blood / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D009206:Myocardium / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D066244:Proton Magnetic Resonance Spectroscopy; D051381:Rats; D013482:Superoxide Dismutase / Q000097:blood / Q000378:metabolism
chemical_list: D002316:Cardiotonic Agents; D011134:Polysaccharides; D008315:Malondialdehyde; D007770:L-Lactate Dehydrogenase; D013482:Superoxide Dismutase; D003402:Creatine Kinase; D052279:Creatine Kinase, MB Form
keywords: Characterization and cardioprotective activity; Endothelium corneum gigeriae Galli; Polysaccharide