title: Adipose stromal cells repair pressure ulcers in both young and elderly mice: potential role of adipogenesis in skin repair.
abstract: More than 2.5 million patients in the U.S. require treatment for pressure ulcers annually, and the elderly are at particularly high risk for pressure ulcer development. Current therapy for pressure ulcers consists of conservative medical management for shallow lesions and aggressive debridement and surgery for deeper lesions. The current study uses a murine model to address the hypothesis that adipose-derived stromal/stem cell (ASC) treatment would accelerate and enhance pressure ulcer repair. The dorsal skin of both young (2 months old [mo]) and old (20 mo) C57BL/6J female mice was sandwiched between external magnets for 12 hours over 2 consecutive days to initiate a pressure ulcer. One day following the induction, mice were injected with ASCs isolated from congenic mice transgenic for the green fluorescent protein under a ubiquitous promoter. Relative to phosphate-buffered saline-treated controls, ASC-treated mice displayed a cell concentration-dependent acceleration of wound closure, improved epidermal/dermal architecture, increased adipogenesis, and reduced inflammatory cell infiltration. The ASC-induced improvements occurred in both young and elderly recipients, although the expression profile of angiogenic, immunomodulatory, and reparative mRNAs differed as a function of age. The results are consistent with clinical reports that fat grafting improved skin architecture in thermal injuries; the authors of this published study have invoked ASC-based mechanisms to account for their clinical outcomes. Thus, the current proof-of-principle study sets the stage for clinical translation of autologous and/or allogeneic ASC treatment of pressure ulcers.
  
  
  CONCLUSIONS
  Adipose-derived stromal/stem cells (ASCs) promote the healing of pressure ulcer wounds in both young and old mice. ASCs enhance wound healing rates through adipogenic differentiation and regeneration of the underlying architecture of the skin.
authors.affiliation: Center for Stem Cell Research and Regenerative Medicine and Department of Pharmacology, Tulane University School of Medicine, New Orleans, Louisiana, USA; LaCell LLC, New Orleans, Louisiana, USA; Division of Plastic and Reconstructive Surgery, Department of Surgery, University of Florida, Gainesville, Florida, USA; Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Olsztyn, Poland; Departments of Medicine, Surgery, and Structural and Cellular Biology, Tulane Health Sciences Center, New Orleans, Louisiana, USA.
authors.forename: Amy L
authors.initials: AL
authors.lastname: Strong
authors.affiliation: Center for Stem Cell Research and Regenerative Medicine and Department of Pharmacology, Tulane University School of Medicine, New Orleans, Louisiana, USA; LaCell LLC, New Orleans, Louisiana, USA; Division of Plastic and Reconstructive Surgery, Department of Surgery, University of Florida, Gainesville, Florida, USA; Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Olsztyn, Poland; Departments of Medicine, Surgery, and Structural and Cellular Biology, Tulane Health Sciences Center, New Orleans, Louisiana, USA.
authors.forename: Annie C
authors.initials: AC
authors.lastname: Bowles
authors.affiliation: Center for Stem Cell Research and Regenerative Medicine and Department of Pharmacology, Tulane University School of Medicine, New Orleans, Louisiana, USA; LaCell LLC, New Orleans, Louisiana, USA; Division of Plastic and Reconstructive Surgery, Department of Surgery, University of Florida, Gainesville, Florida, USA; Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Olsztyn, Poland; Departments of Medicine, Surgery, and Structural and Cellular Biology, Tulane Health Sciences Center, New Orleans, Louisiana, USA.
authors.forename: Connor P
authors.initials: CP
authors.lastname: MacCrimmon
authors.affiliation: Center for Stem Cell Research and Regenerative Medicine and Department of Pharmacology, Tulane University School of Medicine, New Orleans, Louisiana, USA; LaCell LLC, New Orleans, Louisiana, USA; Division of Plastic and Reconstructive Surgery, Department of Surgery, University of Florida, Gainesville, Florida, USA; Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Olsztyn, Poland; Departments of Medicine, Surgery, and Structural and Cellular Biology, Tulane Health Sciences Center, New Orleans, Louisiana, USA.
authors.forename: Trivia P
authors.initials: TP
authors.lastname: Frazier
authors.affiliation: Center for Stem Cell Research and Regenerative Medicine and Department of Pharmacology, Tulane University School of Medicine, New Orleans, Louisiana, USA; LaCell LLC, New Orleans, Louisiana, USA; Division of Plastic and Reconstructive Surgery, Department of Surgery, University of Florida, Gainesville, Florida, USA; Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Olsztyn, Poland; Departments of Medicine, Surgery, and Structural and Cellular Biology, Tulane Health Sciences Center, New Orleans, Louisiana, USA.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Center for Stem Cell Research and Regenerative Medicine and Department of Pharmacology, Tulane University School of Medicine, New Orleans, Louisiana, USA; LaCell LLC, New Orleans, Louisiana, USA; Division of Plastic and Reconstructive Surgery, Department of Surgery, University of Florida, Gainesville, Florida, USA; Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Olsztyn, Poland; Departments of Medicine, Surgery, and Structural and Cellular Biology, Tulane Health Sciences Center, New Orleans, Louisiana, USA.
authors.forename: Xiying
authors.initials: X
authors.lastname: Wu
authors.affiliation: Center for Stem Cell Research and Regenerative Medicine and Department of Pharmacology, Tulane University School of Medicine, New Orleans, Louisiana, USA; LaCell LLC, New Orleans, Louisiana, USA; Division of Plastic and Reconstructive Surgery, Department of Surgery, University of Florida, Gainesville, Florida, USA; Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Olsztyn, Poland; Departments of Medicine, Surgery, and Structural and Cellular Biology, Tulane Health Sciences Center, New Orleans, Louisiana, USA.
authors.forename: Adam J
authors.initials: AJ
authors.lastname: Katz
authors.affiliation: Center for Stem Cell Research and Regenerative Medicine and Department of Pharmacology, Tulane University School of Medicine, New Orleans, Louisiana, USA; LaCell LLC, New Orleans, Louisiana, USA; Division of Plastic and Reconstructive Surgery, Department of Surgery, University of Florida, Gainesville, Florida, USA; Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Olsztyn, Poland; Departments of Medicine, Surgery, and Structural and Cellular Biology, Tulane Health Sciences Center, New Orleans, Louisiana, USA.
authors.forename: Barbara
authors.initials: B
authors.lastname: Gawronska-Kozak
authors.affiliation: Center for Stem Cell Research and Regenerative Medicine and Department of Pharmacology, Tulane University School of Medicine, New Orleans, Louisiana, USA; LaCell LLC, New Orleans, Louisiana, USA; Division of Plastic and Reconstructive Surgery, Department of Surgery, University of Florida, Gainesville, Florida, USA; Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Olsztyn, Poland; Departments of Medicine, Surgery, and Structural and Cellular Biology, Tulane Health Sciences Center, New Orleans, Louisiana, USA.
authors.forename: Bruce A
authors.initials: BA
authors.lastname: Bunnell
authors.affiliation: Center for Stem Cell Research and Regenerative Medicine and Department of Pharmacology, Tulane University School of Medicine, New Orleans, Louisiana, USA; LaCell LLC, New Orleans, Louisiana, USA; Division of Plastic and Reconstructive Surgery, Department of Surgery, University of Florida, Gainesville, Florida, USA; Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Olsztyn, Poland; Departments of Medicine, Surgery, and Structural and Cellular Biology, Tulane Health Sciences Center, New Orleans, Louisiana, USA jeffrey.gimble@lacell-usa.com.
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Gimble
mesh_terms: D050156:Adipogenesis; D000273:Adipose Tissue; D064591:Allografts; D000818:Animals; D064592:Autografts; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D051379:Mice; D003668:Pressure Ulcer / Q000473:pathology / Q000628:therapy*; D012867:Skin / Q000378:metabolism* / Q000473:pathology
keywords: Adipose; Adult stem cells; Mesenchymal stem cells; Stem cells; Stromal cells; Wound healing