title: Separation and purification of five alkaloids from Aconitum duclouxii by counter-current chromatography.
abstract: C19 -diterpenoid alkaloids are the main components of Aconitum duclouxii Levl. The process of separation and purification of these compounds in previous studies was tedious and time consuming, requiring multiple chromatographic steps, thus resulted in low recovery and high cost. In the present work, five C19 -diterpenoid alkaloids, namely, benzoylaconine (1), N-deethylaconitine (2), aconitine (3), deoxyaconitine (4), and ducloudine A (5), were efficiently prepared from A. duclouxii Levl (Aconitum L.) by ethyl acetate extraction followed with counter-current chromatography. In the process of separation, the critical conditions of counter-current chromatography were optimized. The two-phase solvent system composed of n-hexane/ethyl acetate/methanol/water/NH3 ·H2 O (25%) (1:1:1:1:0.1, v/v) was selected and 148.2 mg of 1, 24.1 mg of 2, 250.6 mg of 3, 73.9 mg of 4, and 31.4 mg of 5 were obtained from 1 g total Aconitum alkaloids extract, respectively, in a single run within 4 h. Their purities were found to be 98.4, 97.2, 98.2, 96.8, and 96.6%, respectively, by ultra-high performance liquid chromatography analysis. The presented separation and purification method was simple, fast, and efficient, and the obtained highly pure alkaloids are suitable for biochemical and toxicological investigation.
authors.affiliation: Department of Pharmaceutical Sciences, Zunyi Medical University, Zhuhai, Guangdong, China.
authors.forename: Yarong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Pharmaceutical Sciences, Zunyi Medical University, Zhuhai, Guangdong, China.
authors.forename: Shining
authors.initials: S
authors.lastname: Cai
authors.affiliation: Department of Pharmaceutical Sciences, Zunyi Medical University, Zhuhai, Guangdong, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Chen
authors.affiliation: School of Pharmaceutical Science and Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University Chenggong New City, Kunming, Yunnan, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Deng
authors.affiliation: Department of Pharmaceutical Analysis, Zunyi Medical University, Zunyi, Guizhou, China.
authors.forename: Xumei
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of English, Zunyi Medical University, Zhuhai, Guangdong, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Pharmaceutical Sciences, Zunyi Medical University, Zhuhai, Guangdong, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Pharmaceutical Sciences, Zunyi Medical University, Zhuhai, Guangdong, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xia
authors.affiliation: Department of Pharmaceutical Sciences, Zunyi Medical University, Zhuhai, Guangdong, China.
authors.forename: Mao
authors.initials: M
authors.lastname: Lin
authors.affiliation: Department of Pharmaceutical Sciences, Zunyi Medical University, Zhuhai, Guangdong, China.
authors.forename: Jili
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Pharmaceutical Sciences, Zunyi Medical University, Zhuhai, Guangdong, China.
authors.forename: Weili
authors.initials: W
authors.lastname: Huang
authors.affiliation: Department of Pharmaceutical Sciences, Zunyi Medical University, Zhuhai, Guangdong, China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Pharmaceutical Sciences, Zunyi Medical University, Zhuhai, Guangdong, China.
authors.forename: Canhui
authors.initials: C
authors.lastname: Xiang
authors.affiliation: Department of Pharmaceutical Sciences, Zunyi Medical University, Zhuhai, Guangdong, China.
authors.forename: Guozhen
authors.initials: G
authors.lastname: Cui
authors.affiliation: Department of Pharmaceutical Sciences, Zunyi Medical University, Zhuhai, Guangdong, China.
authors.forename: Lianfeng
authors.initials: L
authors.lastname: Du
authors.affiliation: Department of Pharmaceutical Sciences, Zunyi Medical University, Zhuhai, Guangdong, China.
authors.forename: Huan
authors.initials: H
authors.lastname: He
authors.affiliation: Department of Pharmaceutical Sciences, Zunyi Medical University, Zhuhai, Guangdong, China.
authors.forename: Baohui
authors.initials: B
authors.lastname: Qi
mesh_terms: D031841:Aconitum / Q000737:chemistry*; D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003377:Countercurrent Distribution / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000470:Alkaloids
keywords: Aconitum duclouxii L; C19-diterpenoid alkaloids; Counter-current chromatography