title: Co-digestion of tobacco waste with different agricultural biomass feedstocks and the inhibition of tobacco viruses by anaerobic digestion.
abstract: Tobacco is widely planted across the world especially in China, which means that a large amount of tobacco waste needs to be treated. This study investigated the biogas fermentation of tobacco stalks co-digested with different biomass feedstocks and the inactivation of Tobacco mosaic virus (TMV), Cucumber mosaic virus (CMV) by anaerobic digestion. Results showed that the maximum methane yield of tobacco stalks at 35 °C was 0.163 m(3) CH4 ⋅ kg VS(-1), which was from the co-digestion of tobacco stalks, wheat stalks and pig manure. The largest VS removal rate of tobacco stalks was 59.10%. Proven by indicator paper stripe, half-leaf lesion and RT-PCR, CMV could be inactivated by mesophilic and thermophilic anaerobic digestion, whereas TMV could be only inactivated by thermophilic anaerobic digestion over 20 days. These results suggested that using tobacco stalks as feedstock for anaerobic digestion and applying the digested residue and slurry to Solanaceae crop land are feasible.
authors.affiliation: Biogas Institute of Ministry of Agriculture, Chengdu 610041, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Tobacco Biology and Processing, Ministry of Agriculture, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Dong
authors.affiliation: Biogas Institute of Ministry of Agriculture, Chengdu 610041, China.
authors.forename: Gangjin
authors.initials: G
authors.lastname: Liu
authors.affiliation: Biogas Institute of Ministry of Agriculture, Chengdu 610041, China.
authors.forename: Hongnan
authors.initials: H
authors.lastname: Yang
authors.affiliation: Key Laboratory of Tobacco Biology and Processing, Ministry of Agriculture, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Biogas Institute of Ministry of Agriculture, Chengdu 610041, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Wang
authors.affiliation: Biogas Institute of Ministry of Agriculture, Chengdu 610041, China.
authors.forename: Chuixue
authors.initials: C
authors.lastname: Kong
authors.affiliation: Biogas Institute of Ministry of Agriculture, Chengdu 610041, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Tobacco Biology and Processing, Ministry of Agriculture, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China.
authors.forename: Jinguang
authors.initials: J
authors.lastname: Yang
authors.affiliation: Biogas Institute of Ministry of Agriculture, Chengdu 610041, China.
authors.forename: Liangwei
authors.initials: L
authors.lastname: Deng
authors.affiliation: Key Laboratory of Tobacco Biology and Processing, Ministry of Agriculture, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China. Electronic address: wangshusheng@caas.cn.
authors.forename: Shusheng
authors.initials: S
authors.lastname: Wang
mesh_terms: D000383:Agriculture; D000693:Anaerobiosis; D056804:Biofuels; D018533:Biomass; D005285:Fermentation; D007700:Kinetics; D008697:Methane / Q000096:biosynthesis; D009029:Mosaic Viruses / Q000302:isolation & purification / Q000502:physiology*; D012037:Refuse Disposal / Q000379:methods*; D014026:Nicotiana / Q000737:chemistry* / Q000821:virology*; D014835:Volatilization; D014866:Waste Products / Q000032:analysis*
chemical_list: D056804:Biofuels; D014866:Waste Products; D008697:Methane
keywords: Anaerobic digestion; Cucumber mosaic virus; Methane yield; Tobacco mosaic virus; Tobacco stalks