title: Improved regeneration potential of fibroblasts using ascorbic acid-blended nanofibrous scaffolds.
abstract: Two-dimensional scaffolds, three-dimensional scaffolds, and dermal substitutes are extensively used for biomedical applications in skin tissue regeneration. Not much explored synthetic polymers, like poly(l-lactic acid)-co-poly-(ε-caprolactone) (PLACL), natural polymers, like silk fibroin (SF), and active inducing agents, such as ascorbic acid (AA) and tetracycline hydrochloride (TCH), represent a favorable matrix for fabricating dermal substitutes to engineer artificial skin for wound repair. The profligate nature of residing skin cells near the wound site is a paramount to survival and also regulating stem cells and other cellular networks and mechanical forces. PLACL/SF/TCH/AA nanofibrous scaffolds were fabricated by electrospinning and characterized for fiber morphology, membrane porosity, wettability, and significant subchains using Fourier transform infrared spectroscopy for culturing human-derived dermal fibroblasts. The PLACL, PLACL/SF, PLACL/SF/TCH, and PLACL/SF/TCH/AA scaffolds obtained diameters between 250 and 340 nm. The secretion of collagen by the laboratory-grown fibroblasts over the AA-blended scaffolds was found to be significantly higher compared with that of other scaffolds. The obtained results positively prove that introduction of naturally secreting compounds (AA) by the cells into the nanofibrous scaffolds will favor cell's microenvironment and eventually leads to complete tissue regeneration.
authors.affiliation: Center for Nanofibers and Nanotechnology, NUSNNI, Faculty of Engineering, National University of Singapore, Singapore.|Department of Genetic Engineering, SRM University, Kattankulathur, Chennai, Tamilnadu, India.
authors.forename: Sreepathy
authors.initials: S
authors.lastname: Sridhar
authors.affiliation: Center for Nanofibers and Nanotechnology, NUSNNI, Faculty of Engineering, National University of Singapore, Singapore.
authors.forename: Jayarama Reddy
authors.initials: JR
authors.lastname: Venugopal
authors.affiliation: Center for Nanofibers and Nanotechnology, NUSNNI, Faculty of Engineering, National University of Singapore, Singapore.
authors.forename: Seeram
authors.initials: S
authors.lastname: Ramakrishna
mesh_terms: D000199:Actins / Q000378:metabolism; D001205:Ascorbic Acid / Q000494:pharmacology*; D001391:Azo Compounds / Q000378:metabolism; D001672:Biocompatible Materials / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D003094:Collagen / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D005452:Fluoresceins / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D006801:Humans; D019344:Lactic Acid / Q000494:pharmacology; D008422:Materials Testing; D055595:Mechanical Phenomena / Q000187:drug effects; D018613:Microscopy, Confocal; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D011100:Polyglycolic Acid / Q000494:pharmacology; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D012038:Regeneration / Q000187:drug effects*; D017550:Spectroscopy, Fourier Transform Infrared; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000199:Actins; D001391:Azo Compounds; D001672:Biocompatible Materials; D005452:Fluoresceins; D005456:Fluorescent Dyes; C009798:C.I. direct red 80; C069306:5-chloromethylfluorescein; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D003094:Collagen; D001205:Ascorbic Acid
keywords: PLACL; ascorbic acid; nanofibrous scaffolds; silk fibroin; skin tissue engineering; tetracycline hydrochloride