title: The association of level of reduction of Wilms' tumor gene 1 mRNA transcript in bone marrow and outcome in acute myeloid leukemia patients.
abstract: We focused on the level of reduction of Wilms' tumor gene 1 (WT1) mRNA in bone marrow as minimal residual disease during chemotherapies in adult acute myeloid leukemia (AML) patients. Forty-eight patients were enrolled in this study. Log levels of reduction of WT1 mRNA transcript after induction therapy compared with those at diagnosis were associated with disease-free survival (DFS) (P=0.0066) and overall survival (OS) (P=0.0074) in patients who achieved complete remission. Also log levels of reduction of WT1 mRNA transcript after final consolidation therapy compared with those at diagnosis were associated with DFS (P=0.015) and OS (P=0.012). By multivariate analysis, log levels of reduction of WT1 mRNA transcript after induction therapy and after final consolidation therapy compared with those at diagnosis were extracted as risk factors for outcome. Our results suggest that early and deep reduction of tumor burden may be important for the outcome of AML patients. In addition, it may be useful for the decision to proceed with allogeneic SCT as post-remission therapy.
authors.affiliation: Department of Stem Cell Transplantation, Niigata University Medical and Dental Hospital, Niigata, Japan. Electronic address: ya-shiba@med.niigata-u.ac.jp.
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Shibasaki
authors.affiliation: Niigata Prefectural Shibata Hospital, Shibata, Niigata, Japan.
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Seki
authors.affiliation: Department of Hematology, Niigata University Faculty of Medicine, Niigata, Japan.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Tanaka
authors.affiliation: Department of Hematology, Niigata University Faculty of Medicine, Niigata, Japan.
authors.forename: Syukuko
authors.initials: S
authors.lastname: Miyakoshi
authors.affiliation: Department of Hematology, Niigata University Faculty of Medicine, Niigata, Japan.
authors.forename: Kyoko
authors.initials: K
authors.lastname: Fuse
authors.affiliation: Department of Hematology, Niigata University Faculty of Medicine, Niigata, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kozakai
authors.affiliation: Department of Hematology, Niigata University Faculty of Medicine, Niigata, Japan.
authors.forename: Hironori
authors.initials: H
authors.lastname: Kobayashi
authors.affiliation: Department of Hematology, Niigata University Faculty of Medicine, Niigata, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Ushiki
authors.affiliation: Niigata City General Hospital, Niigata, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Abe
authors.affiliation: Nagaoka Red Cross Hospital, Nagaoka, Niigata, Japan.
authors.forename: Toshio
authors.initials: T
authors.lastname: Yano
authors.affiliation: Niigata Prefectural Shibata Hospital, Shibata, Niigata, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Moriyama
authors.affiliation: Nagaoka Red Cross Hospital, Nagaoka, Niigata, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kuroha
authors.affiliation: Niigata Prefectural Central Hospital, Joetsu, Niigata, Japan.
authors.forename: Noriatsu
authors.initials: N
authors.lastname: Isahai
authors.affiliation: Department of Hematology, Niigata University Faculty of Medicine, Niigata, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Takizawa
authors.affiliation: Department of Hematology, Niigata University Faculty of Medicine, Niigata, Japan.
authors.forename: Miwako
authors.initials: M
authors.lastname: Narita
authors.affiliation: Saiseikai Niigata Second Hospital, Niigata, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Koyama
authors.affiliation: Department of Stem Cell Transplantation, Niigata University Medical and Dental Hospital, Niigata, Japan; Nagaoka Red Cross Hospital, Nagaoka, Niigata, Japan.
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Furukawa
authors.affiliation: Department of Hematology, Niigata University Faculty of Medicine, Niigata, Japan.
authors.forename: Hirohito
authors.initials: H
authors.lastname: Sone
authors.affiliation: Department of Stem Cell Transplantation, Niigata University Medical and Dental Hospital, Niigata, Japan.
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Masuko
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001853:Bone Marrow / Q000378:metabolism / Q000473:pathology; D060830:Consolidation Chemotherapy; D018572:Disease-Free Survival; D006801:Humans; D060828:Induction Chemotherapy; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism*; D012334:RNA, Neoplasm / Q000378:metabolism*; D012307:Risk Factors; D015996:Survival Rate; D025721:WT1 Proteins / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D012334:RNA, Neoplasm; D025721:WT1 Proteins; C583751:WT1 protein, human
keywords: AML; Level of reduction of WT1; Minimal residual disease; Prognosis; WT1