title: Porous alumina, zirconia and alumina/zirconia for bone repair: fabrication, mechanical and in vitro biological response.
abstract: Zirconia (ZrO2) and alumina (Al2O3) based ceramics are widely used for load-bearing applications in bone repair due to their excellent mechanical properties and biocompatibility. They are often regarded as bioinert since no direct bone-material interface is created unless a porous structure intercedes, leading to better bone bonding. In this regard, investigating interactions between cells and porous ceramics is of great interest. In the present study, we report on the successful fabrication of sintered alumina A-61, zirconia Z-50 and zirconia/alumina composite ZA-60 ceramics with medium porosities of 61, 50 and 60%, respectively, indicating a bimodal pore size distribution and good interconnectivity. They exhibit elastic moduli of 3-10 GPa and compressive strength values of 60-240 MPa, similar to those of human cortical bone.We performed in vitro cell-material investigations comparing the adhesion, proliferation and differentiation of mouse pre-osteoblasts MC3T3-E1 on the three porous materials. While all three ceramics demonstrate a strong cell attachment, better cell spreading is observed on zirconia-containing substrates. Significantly higher cell growth was quantified on the latter ceramics, revealing an increased alkaline phosphatase activity, higher collagen production and increased calcium biomineralization compared to A-61. Hence, these porous zirconia-containing ceramics elicit superior biological responses over porous alumina of similar porosity, promoting enhanced biological interaction, with potential use as non-degradable bone grafts or as implant coatings.
authors.affiliation: Dept. of Materials Science and Technology, University of Crete, PO Box 2208, 71003 Heraklio, Greece.
authors.forename: Chrystalleni
authors.initials: C
authors.lastname: Hadjicharalambous
authors.forename: Ales
authors.initials: A
authors.lastname: Buyakov
authors.forename: Svetlana
authors.initials: S
authors.lastname: Buyakova
authors.forename: Sergey
authors.initials: S
authors.lastname: Kulkov
authors.forename: Maria
authors.initials: M
authors.lastname: Chatzinikolaidou
mesh_terms: D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000378:metabolism; D000537:Aluminum Oxide / Q000737:chemistry*; D000818:Animals; D001696:Biomechanical Phenomena; D001861:Bone Regeneration; D018786:Bone Substitutes / Q000737:chemistry*; D002113:Calcification, Physiologic; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D002516:Ceramics / Q000737:chemistry; D003094:Collagen / Q000096:biosynthesis; D006801:Humans; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology; D016062:Porosity; D015040:Zirconium / Q000737:chemistry*
chemical_list: D018786:Bone Substitutes; D003094:Collagen; D015040:Zirconium; D000469:Alkaline Phosphatase; D000537:Aluminum Oxide; C028541:zirconium oxide