title: Protective effect of proteins derived from Calotropis procera latex against acute inflammation in rat.
abstract: The non-dialysable proteins present in the latex of plant Calotropis procera possess anti-inflammatory and analgesic properties. The aim of this study was to evaluate the effect of latex proteins (LP) on the level of inflammatory mediators, oxidative stress markers and tissue histology in the rat model of carrageenan-induced acute inflammation. This study also aimed at evaluating the anti-inflammatory efficacy of LP against different mediators and comparing it with their respective antagonists. Paw inflammation was induced by subplantar injection of carrageenan, and the effect of LP was evaluated on oedema volume, level of TNF-α, PGE(2), myeloperoxidase, nitric oxide, reduced glutathione, thiobarbituric acid-reactive substances and tissue histology at the time of peak inflammation. Paw inflammation was also induced by histamine, serotonin, bradykinin and PGE(2), and the inhibitory effect of LP against these mediators was compared with their respective antagonists at the time of peak effect. Treatment with LP produced a dose-dependent inhibition of oedema formation, and its anti-inflammatory effect against carrageenan-induced paw inflammation was accompanied by reduction in the levels of inflammatory mediators, oxidative stress markers and normalization of tissue architecture. LP also produced a dose-dependent inhibition of oedema formation induced by different inflammatory mediators, and its efficacy was comparable to their respective antagonists and more pronounced than that of diclofenac. Thus, our study shows that LP has a potential to be used for the treatment of various inflammatory conditions where the role of these mediators is well established.
authors.affiliation: Department of Pharmacology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, 110 029, India.
authors.forename: V L
authors.initials: VL
authors.lastname: Kumar
authors.affiliation: Department of Pharmacology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, 110 029, India.
authors.forename: B
authors.initials: B
authors.lastname: Guruprasad
authors.affiliation: Department of Pharmacology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, 110 029, India.
authors.forename: P
authors.initials: P
authors.lastname: Chaudhary
authors.affiliation: Department of Pharmacology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, 110 029, India.
authors.forename: S M A
authors.initials: SM
authors.lastname: Fatmi
authors.affiliation: Centro Universitário Estácio de Sá Via Corpus Rua Eliseu Uchoa Becco, n°600 - Bairro Água Fria CEP:, 60810-270, Fortaleza, Ceará, Brazil.
authors.forename: R S B
authors.initials: RS
authors.lastname: Oliveira
authors.affiliation: Departmento de Bioquimica de Biologia Molecular, Universidade Federal do Ceara, Campus do Pici, Cx. Postal 6033, Fortaleza-Ce Brasil, CEP, 60451-970, Brazil.
authors.forename: M V
authors.initials: MV
authors.lastname: Ramos
mesh_terms: D000208:Acute Disease; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use*; D031107:Calotropis / Q000737:chemistry*; D002351:Carrageenan; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000473:pathology; D005260:Female; D005978:Glutathione / Q000378:metabolism; D007249:Inflammation / Q000188:drug therapy* / Q000517:prevention & control; D007840:Latex / Q000627:therapeutic use*; D008297:Male; D010940:Plant Proteins / Q000627:therapeutic use; D020011:Protective Agents / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D007840:Latex; D010940:Plant Proteins; D020011:Protective Agents; D017392:Thiobarbituric Acid Reactive Substances; D002351:Carrageenan; D005978:Glutathione
keywords: Calotropis procera; inflammation; latex proteins; mediators