title: Mycobacterium bovis infection in the lion (Panthera leo): Current knowledge, conundrums and research challenges.
abstract: Mycobacterium bovis has global public-health and socio-economic significance and can infect a wide range of species including the lion (Panthera leo) resulting in tuberculosis. Lions are classified as vulnerable under the IUCN Red List of Threatened Species and have experienced a 30% population decline in the past two decades. However, no attempt has been made to collate and critically evaluate the available knowledge of M. bovis infections in lions and potential effects on population. In this review we set out to redress this. Arguments suggesting that ingestion of infected prey animals are the main route of infection for lions have not been scientifically proven and research is needed into other possible sources and routes of infection. The paucity of knowledge on host susceptibility, transmission directions and therefore host status, manifestation of pathology, and epidemiology of the disease in lions also needs to be addressed. Advances have been made in diagnosing the presence of M. bovis in lions. However, these diagnostic tests are unable to differentiate between exposure, presence of infection, or stage of disease. Furthermore, there are contradictory reports on the effects of M. bovis on lion populations with more data needed on disease dynamics versus the lion population's reproductive dynamics. Knowledge on disease effects on the lion reproduction and how additional stressors such as drought or co-morbidities may interact with tuberculosis is also lacking. Filling these knowledge gaps will contribute to the understanding of mycobacterial infections and disease in captive and wild lions and assist in lion conservation endeavours.
authors.affiliation: SA MRC Centre for TB Research, DST/NRF Centre of Excellence for Biomedical Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Francie van Zijl Drive, Tygerberg, 7505, South Africa; Mammal Research Institute, Department of Zoology and Entomology, University of Pretoria, Lynwood Road, Pretoria, 0002, South Africa. Electronic address: iggie@shawu.co.za.
authors.forename: Ignatius M
authors.initials: IM
authors.lastname: Viljoen
authors.affiliation: SA MRC Centre for TB Research, DST/NRF Centre of Excellence for Biomedical Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Francie van Zijl Drive, Tygerberg, 7505, South Africa.
authors.forename: Paul D
authors.initials: PD
authors.lastname: van Helden
authors.affiliation: Mammal Research Institute, Department of Zoology and Entomology, University of Pretoria, Lynwood Road, Pretoria, 0002, South Africa; MRC Receptor Biology Unit, IDM, University of Cape Town, Observatory, 7935, South Africa.
authors.forename: Robert P
authors.initials: RP
authors.lastname: Millar
mesh_terms: D000818:Animals; D056727:Endangered Species; D008045:Lions; D009163:Mycobacterium bovis; D011157:Population Dynamics; D012106:Research; D014376:Tuberculosis / Q000175:diagnosis / Q000209:etiology / Q000503:physiopathology / Q000662:veterinary*
keywords: Bovine tuberculosis; Lion; Mycobacterial disease; Mycobacterium bovis; Panthera leo; Wildlife conservation