title: Adipose tissue-derived stem cell therapy in rat cryopreserved ovarian grafts.
abstract: The preliminary results of ovarian transplantation in clinical practice are encouraging. However, the follicular depletion caused by ischemic injury is a main concern and is directly related to short-term graft survival. Cell therapy with adipose tissue-derived stem cells (ASCs) could be an alternative to induce early angiogenesis in the graft. This study aimed to evaluate ASCs therapy in rat cryopreserved ovarian grafts. A single dose of rat ASC (rASCs) or vehicle was injected into the bilateral cryopreserved ovaries of twelve adult female rats immediately after an autologous transplant. Daily vaginal smears were performed for estrous cycle evaluation until euthanasia on postoperative day 30. Follicle viability, graft morphology and apoptosis were assessed. No differences were found with respect to estrous cycle resumption and follicle viability (P>0.05). However, compared with the vehicle-treated grafts, the morphology of the ASCs-treated grafts was impaired, with diffuse atrophy and increased apoptosis (P<0.05). ASCs direct injected in the stroma of rat cryopreserved ovarian grafts impaired its morphology although may not interfere with the functional resumption on short-term. Further investigations are necessary to evaluated whether it could compromise their viability in the long-term.
authors.affiliation: Gynecology Discipline, Laboratory of Structural and Molecular Gynecology (LIM-58), Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, Dr Arnaldo av 455, 2nd floor, room 2113, Pacaembu, São Paulo, 01246-903, Brazil. lucianadamous@gmail.com.|, Galvão Bueno St, 499, Bloco A. Apto31, Liberdade, São Paulo, 01506-000, Brazil. lucianadamous@gmail.com.
authors.forename: Luciana Lamarão
authors.initials: LL
authors.lastname: Damous
authors.affiliation: Laboratory of Genetics and Molecular Cardiology, Heart Institute (Incor), Faculdade de Medicina da Universidade de São Paulo, Dr Enéas de Carvalho Aguiar Av 44, 10th floor, Cerqueira Cesar, São Paulo, 05403-000, Brazil. juliana.nakamuta@gmail.com.
authors.forename: Juliana Sanajotti
authors.initials: JS
authors.lastname: Nakamuta
authors.affiliation: Laboratory of Genetics and Molecular Cardiology, Heart Institute (Incor), Faculdade de Medicina da Universidade de São Paulo, Dr Enéas de Carvalho Aguiar Av 44, 10th floor, Cerqueira Cesar, São Paulo, 05403-000, Brazil. anaelisafb@yahoo.com.br.
authors.forename: Ana Elisa Teófilo Saturi
authors.initials: AE
authors.lastname: de Carvalho
authors.affiliation: Gynecology Discipline, Laboratory of Structural and Molecular Gynecology (LIM-58), Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, Dr Arnaldo av 455, 2nd floor, room 2113, Pacaembu, São Paulo, 01246-903, Brazil. jsoares415@hotmail.com.
authors.forename: José Maria
authors.initials: JM
authors.lastname: Soares
authors.affiliation: Department of Morphology and Genetics, Universidade Federal de São Paulo (UNIFESP), Botucatu St 740. Ed. Lemos Torres, 2nd floor, Vila Clementino, São Paulo, 04023-009, Brazil. manuel.morf@epm.br.
authors.forename: Manuel
authors.initials: M
authors.lastname: de Jesus Simões
authors.affiliation: Laboratory of Genetics and Molecular Cardiology, Heart Institute (Incor), Faculdade de Medicina da Universidade de São Paulo, Dr Enéas de Carvalho Aguiar Av 44, 10th floor, Cerqueira Cesar, São Paulo, 05403-000, Brazil. krieger@incor.usp.br.
authors.forename: José Eduardo
authors.initials: JE
authors.lastname: Krieger
authors.affiliation: Gynecology Discipline, Laboratory of Structural and Molecular Gynecology (LIM-58), Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, Dr Arnaldo av 455, 2nd floor, room 2113, Pacaembu, São Paulo, 01246-903, Brazil. ecbaracat@usp.br.
authors.forename: Edmund C
authors.initials: EC
authors.lastname: Baracat
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D064987:Cell- and Tissue-Based Therapy / Q000379:methods*; D015925:Cryopreservation; D030762:Estrous Cycle / Q000502:physiology; D005260:Female; D006085:Graft Survival; D007511:Ischemia / Q000473:pathology / Q000628:therapy; D018919:Neovascularization, Physiologic / Q000502:physiology; D010053:Ovary / Q000098:blood supply* / Q000637:transplantation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000473:pathology / Q000628:therapy; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology; D014182:Transplantation, Autologous