title: Identification of the proliferative effect of Smad2 and 3 in the TGF β2/Smad signaling pathway using RNA interference in a glioma cell line.
abstract: Gliomas are the most frequently occurring primary tumor in the brain. The most malignant form of glioma, glioblastoma multiforme (GBM), is characterized by rapid and invasive growth and is restricted to the central nervous system (CNS). The transforming growth factor β2 (TGFβ2)/small mothers against decapentaplegic (Smad) signaling pathway is important, not only in GBM cell metabolism and invasion, but also in GBM cell proliferation. However, the functions of the downstream mediators of the TGFβ2/Smads signaling pathway remain to be fully elucidated. In the present study, short hairpin (sh)RNA interference was used to specifically inhibit the expression of Smad2 and Smad3 in the TGFβ2/Smad signaling pathway to investigate the effects of shRNA on the proliferation of human GBM cells. The results demonstrated that knockdown of either Smad2 or Smad3 enhanced cellular proliferation. Additionally, the key target genes involved in GBM cell proliferation, induced by TGFβ2, were found to be dependent on Smad3, but not Smad2.
authors.affiliation: Brain Tumor Research Center, Beijing Neurosurgical Institute and Department of Neurosurgery, Beijing Tian Tan Hospital, Capital Medical University, Beijing 100050, P.R. China.
authors.forename: Chengyuan
authors.initials: C
authors.lastname: Dong
authors.affiliation: Brain Tumor Research Center, Beijing Neurosurgical Institute and Department of Neurosurgery, Beijing Tian Tan Hospital, Capital Medical University, Beijing 100050, P.R. China.
authors.forename: Ruifang
authors.initials: R
authors.lastname: Mi
authors.affiliation: Brain Tumor Research Center, Beijing Neurosurgical Institute and Department of Neurosurgery, Beijing Tian Tan Hospital, Capital Medical University, Beijing 100050, P.R. China.
authors.forename: Guishan
authors.initials: G
authors.lastname: Jin
authors.affiliation: Brain Tumor Research Center, Beijing Neurosurgical Institute and Department of Neurosurgery, Beijing Tian Tan Hospital, Capital Medical University, Beijing 100050, P.R. China.
authors.forename: Yiqiang
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Brain Tumor Research Center, Beijing Neurosurgical Institute and Department of Neurosurgery, Beijing Tian Tan Hospital, Capital Medical University, Beijing 100050, P.R. China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Brain Tumor Research Center, Beijing Neurosurgical Institute and Department of Neurosurgery, Beijing Tian Tan Hospital, Capital Medical University, Beijing 100050, P.R. China.
authors.forename: Fusheng
authors.initials: F
authors.lastname: Liu
mesh_terms: D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005910:Glioma / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008856:Microscopy, Fluorescence; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction; D051899:Smad2 Protein / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D051900:Smad3 Protein / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D053781:Transforming Growth Factor beta2 / Q000378:metabolism*
chemical_list: D034741:RNA, Small Interfering; C497782:SMAD2 protein, human; D051899:Smad2 Protein; D051900:Smad3 Protein; D053781:Transforming Growth Factor beta2