title: Expression of vascular endothelial growth factor in Juvenile Angiofibroma.
abstract: OBJECTIVE
  To examine Juvenile Angiofibroma (JA) tissue for expression of vascular endothelial growth factor (VEGF), and to explore its relationship with puberty status, stage, recurrence and the intraoperative blood loss.
  
  
  METHODS
  Retrospective cohort study of 36 histologically proven cases of JA. Minimum follow up period was 3 years. VEGF expression on tumor cells assessed by immunohistochemistry and graded on two criteria--percentage of cells expressing positivity and the intensity of positivity. These two parameters assessed for impact on puberty status, stage, recurrence, and blood loss.
  
  
  RESULTS
  VEGF expression noted on the tumor endothelial cells in 36/36, and on the tumor stromal cells in 34/36. The percentage of cells expressing VEGF and the intensity of expression were not significantly related to puberty status, tumor stage, recurrence, or intra-operative blood loss (p values 0.3-1.0).
  
  
  CONCLUSIONS
  VEGF expression is near universal in JA. Such expression is independent of puberty status and stage, and does not impact on intra operative blood loss and recurrence.
authors.affiliation: Department of Otolaryngology & Head-Neck Surgery, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Hota
authors.affiliation: Department of Pathology, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: Chitra
authors.initials: C
authors.lastname: Sarkar
authors.affiliation: Department of Pathology, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: Siddhartha Datta
authors.initials: SD
authors.lastname: Gupta
authors.affiliation: Department of Otolaryngology & Head-Neck Surgery, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: Rakesh
authors.initials: R
authors.lastname: Kumar
authors.affiliation: Department of Radiodiagnosis, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: Ashu Seith
authors.initials: AS
authors.lastname: Bhalla
authors.affiliation: Department of Otolaryngology & Head-Neck Surgery, All India Institute of Medical Sciences, New Delhi, India. Electronic address: drathakar@gmail.com.
authors.forename: Alok
authors.initials: A
authors.lastname: Thakar
mesh_terms: D000293:Adolescent; D018322:Angiofibroma / Q000378:metabolism* / Q000473:pathology* / Q000601:surgery; D016063:Blood Loss, Surgical; D002648:Child; D042783:Endothelial Cells / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009364:Neoplasm Recurrence, Local / Q000378:metabolism*; D009367:Neoplasm Staging; D011627:Puberty; D012189:Retrospective Studies; D019292:Skull Base Neoplasms / Q000378:metabolism* / Q000473:pathology* / Q000601:surgery; D017154:Stromal Cells / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D042461:Vascular Endothelial Growth Factor A
keywords: Blood loss; Juvenile Angiofibroma; VEGF