title: Oxygen-induced cell migration and on-line monitoring biomarkers modulation of cervical cancers on a microfluidic system.
abstract: In this work, we report an integrated microfluidic device for cell co-culture under different concentrations of oxygen, in which the secreted protein VEGF165 was on-line qualitatively and semi-quantitatively analyzed by functional nucleic acid, hemin, ABTS and peroxide system. This microfluidic platform allowed investigation of various oxygen and distances effect on cell-to-cell communication. Besides, the microfluidic device was used for real-time analysis of VEGF165 protein by aptamer-functionalized microchannels. Under 5% O2 condition, we found that the migration of CaSki cells was faster than the migration of human umbilical vein endothelial cells. However, the migration of CaSki cells was slower than the migration of HUVECs under 15% O2 condition. Moreover, the shorter intercellular distances, the quicker cells migration. Furthermore, HIF-1α and VEGF165 genes, ROS were analyzed, and the results would provide new perspectives for the diagnosis and medical treatment of cervical cancer.
authors.affiliation: 1] Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084, China [2] School of Science, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Xuexia
authors.initials: X
authors.lastname: Lin
authors.affiliation: 1] Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084, China [2] The Key Laboratory of Bioorganic Phosphorus Chemistry &Chemical Biology, Department of Chemistry, Tsinghua University, Beijing 100084, China.
authors.forename: Qiushui
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084, China.
authors.forename: Wu
authors.initials: W
authors.lastname: Liu
authors.affiliation: Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084, China.
authors.forename: Shiqi
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350005, China.
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: Lin
authors.affiliation: 1] Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084, China [2] The Key Laboratory of Bioorganic Phosphorus Chemistry &Chemical Biology, Department of Chemistry, Tsinghua University, Beijing 100084, China.
authors.forename: Jin-Ming
authors.initials: JM
authors.lastname: Lin
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry; D015415:Biomarkers / Q000032:analysis*; D002450:Cell Communication / Q000187:drug effects; D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D018920:Coculture Techniques; D005260:Female; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000032:analysis / Q000235:genetics; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D010100:Oxygen / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000032:analysis; D002583:Uterine Cervical Neoplasms / Q000378:metabolism / Q000473:pathology; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis / Q000235:genetics
chemical_list: D052157:Aptamers, Nucleotide; D015415:Biomarkers; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D017382:Reactive Oxygen Species; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D010100:Oxygen