title: Secondary structure and (1)H, (13)C, (15)N resonance assignments of the endosomal sorting protein sorting nexin 3.
abstract: Sorting nexin 3 (SNX3) belongs to a sub-family of sorting nexins that primarily contain a single Phox homology domain capable of binding phosphoinositides and membranes. We report the complete (1)H, (13)C and (15)N resonance assignments of the full-length human SNX3 protein and identification of its secondary structure elements, revealing a canonical fold and unstructured termini.
authors.affiliation: Henry Wellcome Building for Biomolecular NMR Spectroscopy, School of Cancer Sciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.
authors.forename: Michael
authors.initials: M
authors.lastname: Overduin
authors.affiliation: Henry Wellcome Building for Biomolecular NMR Spectroscopy, School of Cancer Sciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.
authors.forename: Sandya
authors.initials: S
authors.lastname: Rajesh
authors.affiliation: Department of Biochemistry Sciences II, University of Geneva, 30, Quai Ernest-Ansermet, 1211, Geneva 4, Switzerland.
authors.forename: Jean
authors.initials: J
authors.lastname: Gruenberg
authors.affiliation: Henry Wellcome Building for Biomolecular NMR Spectroscopy, School of Cancer Sciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK. m.lenoir@bham.ac.uk.
authors.forename: Marc
authors.initials: M
authors.lastname: Lenoir
mesh_terms: D000595:Amino Acid Sequence; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D011992:Endosomes / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D009587:Nitrogen Isotopes; D017433:Protein Structure, Secondary; D066244:Proton Magnetic Resonance Spectroscopy; D058305:Sorting Nexins / Q000737:chemistry*
chemical_list: D009587:Nitrogen Isotopes; C513980:SNX3 protein, human; D058305:Sorting Nexins
keywords: Endosome; NMR; PX domain; Phosphatidylinositol 3-phosphate; Phosphoinositide recognition; SNX3