title: A flow cytometer-based whole cell screening toolbox for directed hydrolase evolution through fluorescent hydrogels.
abstract: A high throughput whole cell flow cytometer screening toolbox was developed and validated by identifying improved variants (1.3-7-fold) for three hydrolases (esterase, lipase, cellulase). The screening principle is based on coupled enzymatic reaction using glucose derivatives which yield upon hydrolysis a fluorescent-hydrogel-layer on the surface of E. coli cells.
authors.affiliation: DWI-Leibniz Institut für Interaktive Materialien, Forckenbeckstraße 50, 52056 Aachen, Germany.
authors.forename: Nina
authors.initials: N
authors.lastname: Lülsdorf
authors.forename: Christian
authors.initials: C
authors.lastname: Pitzler
authors.forename: Michael
authors.initials: M
authors.lastname: Biggel
authors.forename: Ronny
authors.initials: R
authors.lastname: Martinez
authors.forename: Ljubica
authors.initials: L
authors.lastname: Vojcic
authors.forename: Ulrich
authors.initials: U
authors.lastname: Schwaneberg
mesh_terms: D004926:Escherichia coli / Q000166:cytology* / Q000378:metabolism; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D020100:Hydrogels / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D006867:Hydrolases / Q000378:metabolism*; D006868:Hydrolysis
chemical_list: D005456:Fluorescent Dyes; D020100:Hydrogels; D006867:Hydrolases