title: Immunization with a live attenuated H7N9 influenza vaccine protects mice against lethal challenge.
abstract: The emergence of severe cases of human influenza A (H7N9) viral infection in China in the spring of 2003 resulted in a global effort to rapidly develop an effective candidate vaccine. In this study, a cold-adapted (ca), live attenuated monovalent reassortant influenza H7N9 virus (Ah01/AA ca) was generated using reverse genetics that contained hemagglutinin (HA) and neuraminidase (NA) genes from a 2013 pandemic A H7N9 isolate, A/Anhui/01/2013 virus (Ah01/H7N9); the remaining six backbone genes derived from the cold-adapted influenza H2N2 A/Ann Arbor/6/60 virus (AA virus). Ah01/AA ca virus exhibited temperature sensitivity (ts), ca, and attenuation (att) phenotypes. Intranasal immunization of female BALB/c mice with Ah01/AA ca twice at a 2-week interval induced robust humoral, mucosal, and cell-mediated immune responses in a dose-dependent manner. Furthermore, the candidate Ah01/AA ca virus was immunogenic and offered partial or complete protection of mice against a lethal challenge by the live 2013 influenza A H7N9 (A/Anhui/01/2013). Protection was demonstrated by the inhibition of viral replication and the attenuation of histopathological changes in the challenged mouse lung. Taken together, these data support the further evaluation of this Ah01/AA ca candidate vaccine in primates.
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing, China.
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Yang
authors.affiliation: China Astronaut Research and Training Center, Beijing, China.
authors.forename: Jianyu
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Beijing 307 Hospital Affiliated to Academy of Medical Sciences, Beijing, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Wang
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing, China.
authors.forename: Yueqiang
authors.initials: Y
authors.lastname: Duan
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing, China.
authors.forename: Zhongpeng
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Chen
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing, China.
authors.forename: Liangyan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xing
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing, China.
authors.forename: Chengcai
authors.initials: C
authors.lastname: Lai
authors.affiliation: Beijing 302 Hospital, Beijing, China.
authors.forename: Shaogeng
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing, China.
authors.forename: Xiliang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing, China; Beijing 302 Hospital, Beijing, China.
authors.forename: Penghui
authors.initials: P
authors.lastname: Yang
mesh_terms: D000818:Animals; D005260:Female; D006801:Humans; D007114:Immunization; D064766:Influenza A Virus, H7N9 Subtype / Q000276:immunology*; D007252:Influenza Vaccines / Q000276:immunology*; D007251:Influenza, Human / Q000517:prevention & control* / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D009976:Orthomyxoviridae Infections / Q000517:prevention & control* / Q000821:virology
chemical_list: D007252:Influenza Vaccines