title: [Recombinant fluorescent models for studying of diphtheria toxin].
abstract: Diphtheria toxin is the main pathogenicity factor of causative agent of diphtheria Corynebacterium diphtheriae. Due to the small molecule size, it is of considerable interestfor the development of synthetic protein molecules with transporting function, e.g. immunotoxins. Expression and characterization of nontoxic recombinant fluorescent derivates of diphtheria toxin and its nontoxic mutant CRM 197 were described in this article. Obtained proteins may be applied in studies of receptor-binding and transporting functions of the toxin in cells, for determination of toxin receptor proHB-EGF expression level, immunization and antibody generation against the toxin and in development of diagnostic test-systems, detection of diphtheria toxin and antitoxic antibodies.
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Labyntsev
authors.forename: N V
authors.initials: NV
authors.lastname: Korotkevich
authors.forename: K Iu
authors.initials: KIu
authors.lastname: Manoĭlov
authors.forename: A A
authors.initials: AA
authors.lastname: Kaberniuk
authors.forename: D V
authors.initials: DV
authors.lastname: Kolibo
authors.forename: S V
authors.initials: SV
authors.lastname: Komisarenko
mesh_terms: D000907:Antibodies, Bacterial / Q000737:chemistry / Q000276:immunology; D003001:Cloning, Molecular; D003353:Corynebacterium diphtheriae / Q000235:genetics / Q000472:pathogenicity; D004167:Diphtheria Toxin / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D007114:Immunization; D008164:Luminescent Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D008958:Models, Molecular; D015202:Protein Engineering; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*
chemical_list: D000907:Antibodies, Bacterial; D004167:Diphtheria Toxin; D008164:Luminescent Proteins; D011994:Recombinant Proteins