title: [Genetic characterisation of Powassan virus (POWV) isolated from Haemophysalis longicornis ticks in Primorye and two strains of Tick-borne encephalitis virus (TBEV) (Flaviviridae, Flavivirus): Alma-Arasan virus (AAV) isolated from Ixodes persulcatus ticks in Kazakhstan and Malyshevo virus isolated from Aedes vexans nipponii mosquitoes in Khabarovsk kray].
abstract: The complete genomes of the three tick-borne flaviviruses (genus Flavivirus, fam. Bunyaviridae) were sequenced: Povassan virus (POWV, strain LEIV-3070Prm, isolated from Haemophysalis logicornis in Primorsky Krai, Russia in 1977), Alma-Arasan virus (AAV, strain LEIV-1380Kaz, isolated from Ixodes persulcatus ticks in Kazakhstan in 1977) and Malyshevo virus (isolated from a pool of Aedes vexans nipponii mosquitoes, in the Khabarovsk Krai, Russia in 1978). It is shown that AAV and Malyshevo virus are the strains of Tick-borne encephalitis virus (TBEV) and belong to Sibirian and Far-Eastern genotypes, respectively (GenBank ID: AAV KJ744033; strain Malyshevo KJ744034). Phylogenetically AAV is closest related (94,6% nt and 98,3% aa identity) to TBEV strains, isolated in Sibiria (Vasilchenko, Aino, Chita-653, Irkutsk-12). Malyshevo virus is closest related (96,4% nt and 98,3% nt identity) to strains of TBEV, isolated in Far Eastern part of Russia (1230, Spassk-72, Primorye-89). POWV LEIV-3070Prm has 99.7% identity with the prototype strain POWV LB, isolated in Canada and 99.5% of isolates with Far-Eastern strains of POWV (Spassk-9 and Nadezdinsk-1991).
authors.forename: D K
authors.initials: DK
authors.lastname: L'vov
authors.forename: S V
authors.initials: SV
authors.lastname: Al'khovskiĭ
authors.forename: M Iu
authors.initials: MIu
authors.lastname: Shchelkanov
authors.forename: P G
authors.initials: PG
authors.lastname: Deriabin
authors.forename: A K
authors.initials: AK
authors.lastname: Gitel'man
authors.forename: A G
authors.initials: AG
authors.lastname: Botikov
authors.forename: V A
authors.initials: VA
authors.lastname: Aristova
mesh_terms: D000330:Aedes / Q000821:virology*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018067:Flaviviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D018178:Flaviviridae Infections / Q000635:transmission / Q000662:veterinary* / Q000821:virology; D016679:Genome, Viral; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D007623:Kazakhstan; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D012689:Sequence Homology, Nucleic Acid; D012800:Siberia; D013987:Ticks / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D014764:Viral Proteins