title: Using gold aggregation to probe the inhibition and destruction of the G-quadruplex structure by TT-dimerization.
abstract: Thrombin binding G-quadruplex oligonucleotide containing two TT-dimer fragments and a gold attachment (ODN G1-G) was designed and synthesized with the aim of understanding the TT-dimer effect in G-quadruplex formation. Our results showed that TT-dimer mutation induced by UV light inhibits the formation of and even destroys the G-quadruplex structure, as confirmed by UV, CD and melting temperature measurements. The structural change resulting from TT-dimer formation with DNA was additionally probed and was found to be accompanied by significant gold aggregation that was observed in the form of a signal change from red to blue.
authors.affiliation: Department of Chemistry, Chonbuk National University, Jeonju 561-756, South Korea.
authors.forename: Han Na
authors.initials: HN
authors.lastname: Joo
authors.affiliation: Department of Chemistry, Chonbuk National University, Jeonju 561-756, South Korea; Department of Bioactive Material Sciences, Research Center of Bioactive Materials, Chonbuk National University, Jeonju 561-756, South Korea. Electronic address: yseo@jbnu.ac.kr.
authors.forename: Young Jun
authors.initials: YJ
authors.lastname: Seo
mesh_terms: D054856:G-Quadruplexes; D006046:Gold / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D013050:Spectrometry, Fluorescence; D013917:Thrombin / Q000737:chemistry*
chemical_list: D009838:Oligodeoxyribonucleotides; D006046:Gold; D013917:Thrombin
keywords: Fluorophore; G-quadruplex structure; Gold aggregation; Probe; TT-dimerization