title: Bottom-Up Engineering of Well-Defined 3D Microtissues Using Microplatforms and Biomedical Applications.
abstract: During the last decades, the engineering of well-defined 3D tissues has attracted great attention because it provides in vivo mimicking environment and can be a building block for the engineering of bioartificial organs. In this Review, diverse engineering methods of 3D tissues using microscale devices are introduced. Recent progress of microtechnologies has enabled the development of microplatforms for bottom-up assembly of diverse shaped 3D tissues consisting of various cells. Micro hanging-drop plates, microfluidic chips, and arrayed microwells are the typical examples. The encapsulation of cells in hydrogel microspheres and microfibers allows the engineering of 3D microtissues with diverse shapes. Applications of 3D microtissues in biomedical fields are described, and the future direction of microplatform-based engineering of 3D micro-tissues is discussed.
authors.affiliation: KU-KIST Graduate School of Converging, Science and Technology, Korea University, Seoul, 136-701, Republic of Korea.
authors.forename: Geon Hui
authors.initials: GH
authors.lastname: Lee
authors.affiliation: KU-KIST Graduate School of Converging, Science and Technology, Korea University, Seoul, 136-701, Republic of Korea.
authors.forename: Jae Seo
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Center of Organ Manufacturing, Department of Mechanical Engineering, Tsinghua University, Beijing, 100084, P. R. China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Biomedical Engineering, College of Health Science, Korea University, Seoul, 136-701, Republic of Korea.
authors.forename: Sang Hoon
authors.initials: SH
authors.lastname: Lee
mesh_terms: D020811:Biomedical Technology / Q000379:methods*; D006801:Humans; D044085:Microfluidics; D055616:Microtechnology / Q000379:methods*; D018874:Spheroids, Cellular / Q000166:cytology; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
keywords: 3D cultures; microtissue; regenerative medicine; self-assembly; tissue engineering