title: A new polyphenol, 1, 3-di-O-caffeoyl-5-O-(1-methoxyl-2-O-caffeoyl-4-maloyl)-quinic acid, isolated from cultured cells of Saussurea involucrata.
abstract: The present study was designed to isolate the polyphenol constituents of cultured cells of Saussurea involucrata. The polyphenol type constituents were isolated using chromatography methods, and then characterized by spectral analysis. 1,1-Diphenyl-2-picrylhydrazyl radical 2,2-Diphenyl-1-(2,4,6-trinitrophenyl)-hydrazyl (DPPH) and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) free radical scavenging were assayed using Vitamin C as the positive control. One new polyphenol 18, 1, 3-di-O-caffeoyl-5-O-(1-methoxyl-2-O-caffeoyl-4-maloyl)-quinic acid, together with 17 known compounds, was isolated and characterized. In conclusion, Compound 18 was a new caffeoyl maloyl quinic acid type polyphenol and showed desired vitro anti-oxidant activity. Compounds 1-5, 9, 10, 14, 15, and 17 were isolated from cultured cells of Saussurea involucrata for the first time.
authors.affiliation: Department of Bioscience and Biotechnology, School of Life science and Biotechnology, Dalian University of Technology, Dalian 116024, China; Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Xiao-Wei
authors.initials: XW
authors.lastname: Zou
authors.affiliation: Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.affiliation: Dalian Practical Biotechnology Co., Ltd., Dalian 116001, China.
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Liu
authors.affiliation: Department of Bioscience and Biotechnology, School of Life science and Biotechnology, Dalian University of Technology, Dalian 116024, China. Electronic address: zhlxiu@dlut.edu.cn.
authors.forename: Zhi-Long
authors.initials: ZL
authors.lastname: Xiu
authors.affiliation: Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; Institute of Chinese Materia Medica, Chinese Academy of Chinese Medical Sciences, Beijing 100700, China. Electronic address: hbxiao@dicp.ac.cn.
authors.forename: Hong-Bin
authors.initials: HB
authors.lastname: Xiao
mesh_terms: D000975:Antioxidants / Q000737:chemistry; D002478:Cells, Cultured; D059808:Polyphenols / Q000737:chemistry / Q000302:isolation & purification*; D030441:Saussurea / Q000737:chemistry*
chemical_list: C000604045:1,3-di-O-caffeoyl-5-O-(1-methoxyl-2-O-caffeoyl-4-maloyl)-quinic acid; D000975:Antioxidants; D059808:Polyphenols
keywords: Cultured cells; Free radical scavenging activity; Polyphenol; Saussurea involucrata