title: Proteomic analysis of fetal programming-related obesity markers.
abstract: The objectives of this study were to analyze fetal programming in rat brain using proteomic analysis and to identify fetal programming-related obesity markers. Sprague-Dawley rats were divided into four feeding groups: (i) the Ad Libitum (AdLib)/AdLib group was given a normal diet during pregnancy and the lactation period; (ii) the AdLib/maternal food restriction group (FR) was subjected to 50% FR during the lactation period; (iii) the FR/AdLib group was subjected to 50% FR during pregnancy; and (iv) the FR/FR group was subjected to 50% FR during pregnancy and the lactation period. Offspring from each group were sacrificed at 3 weeks of age and whole brains were dissected. To obtain a maximum number of protein markers related to obesity, 2DE and Pathway Studio bioinformatics analysis were performed. The identities of the markers among the selected and candidate proteins were confirmed by Western blotting and immunohistochemistry. Proteomic and bioinformatics analyses revealed that expression of ubiquitin carboxy-terminal hydrolase L1 (UCHL1) and Secernin 1 (SCRN1) were significantly different in the FR/AdLib group compared with the AdLib/AdLib group for both male and female offspring. These findings suggest that UCHL1 and SCRN1 may be used as fetal programming-related obesity markers.
authors.affiliation: Department of Obstetrics and Gynecology, Ewha Womans University, Seoul, South Korea.
authors.forename: Ji Hye
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Obstetrics and Gynecology, Ewha Womans University, Seoul, South Korea.
authors.forename: Jae Young
authors.initials: JY
authors.lastname: Yoo
authors.affiliation: Department of Obstetrics and Gynecology, Ewha Womans University, Seoul, South Korea.
authors.forename: Young-Ah
authors.initials: YA
authors.lastname: You
authors.affiliation: Department of Animal Science and Technology, Chung-Ang University, Anseong, Gyeonggi-Do 456-756, South Korea.
authors.forename: Woo-Sung
authors.initials: WS
authors.lastname: Kwon
authors.affiliation: Department of Obstetrics and Gynecology, Ewha Womans University, Seoul, South Korea.
authors.forename: Sang Mi
authors.initials: SM
authors.lastname: Lee
authors.affiliation: Department of Animal Science and Technology, Chung-Ang University, Anseong, Gyeonggi-Do 456-756, South Korea.
authors.forename: Myung-Geol
authors.initials: MG
authors.lastname: Pang
authors.affiliation: Department of Obstetrics and Gynecology, Ewha Womans University, Seoul, South Korea.
authors.forename: Young Ju
authors.initials: YJ
authors.lastname: Kim
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015415:Biomarkers / Q000378:metabolism*; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism; D019295:Computational Biology / Q000379:methods; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D047109:Fetal Development; D007150:Immunohistochemistry; D007774:Lactation; D008297:Male; D039382:Maternal Nutritional Physiological Phenomena; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009765:Obesity / Q000196:embryology / Q000378:metabolism*; D011247:Pregnancy; D020543:Proteome / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D017207:Rats, Sprague-Dawley; D043222:Ubiquitin Thiolesterase / Q000378:metabolism
chemical_list: D015415:Biomarkers; D009419:Nerve Tissue Proteins; D020543:Proteome; C471695:secernin 1 protein, mouse; D043222:Ubiquitin Thiolesterase; C496976:Uchl1 protein, mouse
keywords: Animal proteomics; Fetal programming; Obesity; Secrenin 1; Ubiquitin carboxy-terminal hydrolase L1