title: Using 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG) to study carbon allocation in plants after herbivore attack.
abstract: BACKGROUND
  Although leaf herbivory-induced changes in allocation of recently assimilated carbon between the shoot and below-ground tissues have been described in several species, it is still unclear which part of the root system is affected by resource allocation changes and which signalling pathways are involved. We investigated carbon partitioning in root tissues following wounding and simulated leaf herbivory in young Nicotiana attenuata plants.
  
  
  RESULTS
  Using 2-deoxy-2-[(18)F]fluoro-D-glucose ([(18)F]FDG), which was incorporated into disaccharides in planta, we found that simulated herbivory reduced carbon partitioning specifically to the root tips in wild type plants. In jasmonate (JA) signalling-deficient COI1 plants, the wound-induced allocation of [(18)F]FDG to the roots was decreased, while more [(18)F]FDG was transported to young leaves, demonstrating an important role of the JA pathway in regulating the wound-induced carbon partitioning between shoots and roots.
  
  
  CONCLUSIONS
  Our data highlight the use of [(18)F]FDG to study stress-induced carbon allocation responses in plants and indicate an important role of the JA pathway in regulating wound-induced shoot to root signalling.
authors.affiliation: Department of Molecular Ecology, Max-Planck-Institute for Chemical Ecology, Hans-Knöll-Str.8, 07745, Jena, Germany. smeldau@ice.mpg.de.|German Centre for integrative Biodiversity Research (iDiv), Deutscher Platz 5, 04107, Leipzig, Germany. smeldau@ice.mpg.de.|Present address: KWS SAAT AG, Molecular Physiology, R&D, RD-ME-MP, Grimsehlstrasse 31, D-37555, Einbeck, Germany. smeldau@ice.mpg.de.
authors.forename: Stefan
authors.initials: S
authors.lastname: Meldau
authors.affiliation: Department of Molecular Ecology, Max-Planck-Institute for Chemical Ecology, Hans-Knöll-Str.8, 07745, Jena, Germany. mgw75@cornell.edu.|Present address: Boyce Thompson Institute for Plant Research, 533 Tower Road, Ithaca, 14853, NY, USA. mgw75@cornell.edu.
authors.forename: Melkamu G
authors.initials: MG
authors.lastname: Woldemariam
authors.affiliation: Mass Spectrometry Research Group, Max-Planck-Institute for Chemical Ecology, Hans-Knöll-Str.8, 07745, Jena, Germany. afantagare@ice.mpg.de.
authors.forename: Amol
authors.initials: A
authors.lastname: Fatangare
authors.affiliation: Mass Spectrometry Research Group, Max-Planck-Institute for Chemical Ecology, Hans-Knöll-Str.8, 07745, Jena, Germany. svatos@ice.mpg.de.
authors.forename: Ales
authors.initials: A
authors.lastname: Svatos
authors.affiliation: Department of Molecular Ecology, Max-Planck-Institute for Chemical Ecology, Hans-Knöll-Str.8, 07745, Jena, Germany. igalis@rib.okayama-u.ac.jp.|Present address: Okayama University, Institute of Plant Science and Resources, Chuo 2-20-1, 710-0046, Kurashiki, Japan. igalis@rib.okayama-u.ac.jp.
authors.forename: Ivan
authors.initials: I
authors.lastname: Galis
mesh_terms: D000818:Animals; D001345:Autoradiography; D002244:Carbon / Q000378:metabolism*; D003517:Cyclopentanes / Q000378:metabolism; D004187:Disaccharides / Q000096:biosynthesis; D019788:Fluorodeoxyglucose F18 / Q000378:metabolism*; D060434:Herbivory; D009928:Organ Specificity; D054883:Oxylipins / Q000378:metabolism; D010937:Plant Growth Regulators / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism*; D019275:Radiopharmaceuticals / Q000378:metabolism; D015398:Signal Transduction; D013312:Stress, Physiological; D014026:Nicotiana / Q000378:metabolism*
chemical_list: D003517:Cyclopentanes; D004187:Disaccharides; D054883:Oxylipins; D010937:Plant Growth Regulators; D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18; C011006:jasmonic acid; D002244:Carbon