title: Acute caffeine administration affects zebrafish response to a robotic stimulus.
abstract: Zebrafish has been recently proposed as a valid animal model to investigate the fundamental mechanisms regulating emotional behavior and evaluate the modulatory effects exerted by psychoactive compounds. In this study, we propose a novel methodological framework based on robotics and information theory to investigate the behavioral response of zebrafish exposed to acute caffeine treatment. In a binary preference test, we studied the response of caffeine-treated zebrafish to a replica of a shoal of conspecifics moving in the tank. A purely data-driven information theoretic approach was used to infer the influence of the replica on zebrafish behavior as a function of caffeine concentration. Our results demonstrate that acute caffeine administration modulates both the average speed and the interaction with the replica. Specifically, zebrafish exposed to elevated doses of caffeine show reduced locomotion and increased sensitivity to the motion of the replica. The methodology developed in this study may complement traditional experimental paradigms developed in the field of behavioral pharmacology.
authors.affiliation: Department of Mechanical and Aerospace Engineering, New York University Polytechnic School of Engineering, 6 MetroTech Center, Brooklyn, NY 11201, USA.
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Ladu
authors.affiliation: Department of Mechanical and Aerospace Engineering, New York University Polytechnic School of Engineering, 6 MetroTech Center, Brooklyn, NY 11201, USA.
authors.forename: Violet
authors.initials: V
authors.lastname: Mwaffo
authors.affiliation: Department of Mechanical and Aerospace Engineering, New York University Polytechnic School of Engineering, 6 MetroTech Center, Brooklyn, NY 11201, USA.
authors.forename: Jasmine
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Mechanical and Aerospace Engineering, New York University Polytechnic School of Engineering, 6 MetroTech Center, Brooklyn, NY 11201, USA; Section of Behavioural Neuroscience, Department of Cell Biology and Neuroscience, Istituto Superiore di Sanità, Rome 00161, Italy.
authors.forename: Simone
authors.initials: S
authors.lastname: Macrì
authors.affiliation: Department of Mechanical and Aerospace Engineering, New York University Polytechnic School of Engineering, 6 MetroTech Center, Brooklyn, NY 11201, USA. Electronic address: mporfiri@nyu.edu.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Porfiri
mesh_terms: D000818:Animals; D001007:Anxiety / Q000139:chemically induced / Q000523:psychology; D001522:Behavior, Animal / Q000187:drug effects*; D002110:Caffeine / Q000008:administration & dosage*; D007257:Information Theory; D012371:Robotics; D012919:Social Behavior; D015027:Zebrafish
chemical_list: D002110:Caffeine
keywords: Anxiety; Caffeine; Fish replica; Robotics; Transfer entropy; Zebrafish