title: Production of β -cyclodextrin from pH and thermo stable Cyclodextrin Glycosyl Transferase, obtained from Arthrobacter mysorens and its evaluation as a drug carrier for Irbesartan.
abstract: Cyclodextrins (CDs) are carrier molecules produced by cyclization of α-1,4-glucans by Cyclodextrin Glycosyl Transferase (CGTase). These torus shaped molecules have hydrophobic cavity and hydrophilic shell making them useful in pharmaceutical, food, textile, pesticide and cosmetic industries. In this study, culture conditions for the production of CGTase by organism belonging to Arthrobacter genus obtained from a paddy field soil were optimized by single parameter mode. Soluble starch, yeast extract and magnesium sulphate played an important role in CGTase production. Percentage increase in CGTase yield under optimized conditions was 396.77%. The enzyme precipitated by 60% ammonium sulphate was purified using DEAE-sepharose. The molecular weight of the purified protein as determined by SDS-PAGE was 75 kDa. Purified CGTase was thermostable and stable over a wide pH range. Dissolution studies on β -cyclodextrin-Irbesartan complex revealed that β -CDs formed were useful in preparing immediate release oral dosage forms.
authors.forename: Y
authors.initials: Y
authors.lastname: Rajesh
authors.forename: K
authors.initials: K
authors.lastname: Narayanan
authors.forename: M Sreenivasa
authors.initials: MS
authors.lastname: Reddy
authors.forename: Vijaya K
authors.initials: VK
authors.lastname: Bhaskar
authors.forename: G Gautham
authors.initials: GG
authors.lastname: Shenoy
authors.affiliation: Dept. of Pharmaceutical Biotechnology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal -576 104, India. vm.subra@manipal.edu.
authors.forename: V M
authors.initials: VM
authors.lastname: Subrahmanyam
authors.forename: J Venkata
authors.initials: JV
authors.lastname: Rao
mesh_terms: D047228:Angiotensin II Type 1 Receptor Blockers / Q000737:chemistry*; D001173:Arthrobacter / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D001713:Biphenyl Compounds / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical; D004337:Drug Carriers; D004795:Enzyme Stability; D005964:Glucosyltransferases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D000077405:Irbesartan; D007700:Kinetics; D008970:Molecular Weight; D012995:Solubility; D013678:Technology, Pharmaceutical / Q000379:methods; D013696:Temperature; D013777:Tetrazoles / Q000737:chemistry*; D047392:beta-Cyclodextrins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D047228:Angiotensin II Type 1 Receptor Blockers; D001426:Bacterial Proteins; D001713:Biphenyl Compounds; D004337:Drug Carriers; D013777:Tetrazoles; D047392:beta-Cyclodextrins; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase; D000077405:Irbesartan; C031215:betadex