title: Phospholipase C γ1 regulates early secretory trafficking and cell migration via interaction with p115.
abstract: The role of early secretory trafficking in the regulation of cell motility remains incompletely understood. Here we used a small interfering RNA screen to monitor the effects on structure of the Golgi apparatus and cell migration. Two major Golgi phenotypes were observed-fragmented and small Golgi. The latter exhibited a stronger correlation with a defect in cell migration. Among the small Golgi hits, we focused on phospholipase C γ1 (PLCγ1). We show that PLCγ1 regulates Golgi structure and cell migration independently of its catalytic activity but in a manner that depends on interaction with the tethering protein p115. PLCγ1 regulates the dynamics of p115 in the early secretory pathway, thereby controlling trafficking from the endoplasmic reticulum to the Golgi. Our results uncover a new function of PLCγ1 that is independent of its catalytic function and link early secretory trafficking to the regulation of cell migration.
authors.affiliation: Department of Biology, University of Konstanz, 78457 Konstanz, Germany Biotechnology Institute Thurgau, 8280 Kreuzlingen, Switzerland.
authors.forename: Valentina
authors.initials: V
authors.lastname: Millarte
authors.affiliation: Institut Curie, CNRS UMR 144, 75248 Paris, France.
authors.forename: Gaelle
authors.initials: G
authors.lastname: Boncompain
authors.affiliation: Department of Biology, University of Konstanz, 78457 Konstanz, Germany Biotechnology Institute Thurgau, 8280 Kreuzlingen, Switzerland.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Tillmann
authors.affiliation: Institut Curie, CNRS UMR 144, 75248 Paris, France.
authors.forename: Franck
authors.initials: F
authors.lastname: Perez
authors.affiliation: Department of Cell, Developmental and Integrative Biology, University of Alabama at Birmingham, Birmingham, AL 35294.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Sztul
authors.affiliation: Department of Biology, University of Konstanz, 78457 Konstanz, Germany Biotechnology Institute Thurgau, 8280 Kreuzlingen, Switzerland hesso.farhan@uni-konstanz.de.
authors.forename: Hesso
authors.initials: H
authors.lastname: Farhan
mesh_terms: D002465:Cell Movement / Q000502:physiology*; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism*; D000077614:Golgi Matrix Proteins; D006801:Humans; D051966:Phospholipase C gamma / Q000378:metabolism*; D011485:Protein Binding; D055571:Secretory Pathway / Q000502:physiology*; D033921:Vesicular Transport Proteins / Q000378:metabolism*
chemical_list: D000077614:Golgi Matrix Proteins; D033921:Vesicular Transport Proteins; C091580:vesicular transport factor p115; C522157:PLCG1 protein, human; D051966:Phospholipase C gamma