title: Production of Norovirus VLPs to size homogeneity.
abstract: Expression of full-length major capsid protein of Noroviruses (NoVs) in sf9 cells using recombinant baculovirus expression system leads to the formation of virus-like particles (VLPs) with two sizes. In our pursuit of VLPs with uniform sizes, we find that N terminal truncated capsid protein formed primary VLPs with an average size of 21 nm. This kind of VLPs showed similar binding patterns to those produced with full-length major capsid protein. HBGA-VLPs binding assay and saliva-VLPs blocking analysis, as well as stability test demonstrate that the smaller 21 nm VLPs might be an excellent candidate for NoVs vaccine.
authors.affiliation: Wuhan Institute of Biological Products, Wuhan PR China. Electronic address: 1246105971@qq.com.
authors.forename: Yuqi
authors.initials: Y
authors.lastname: Huo
authors.affiliation: Wuhan Institute of Biological Products, Wuhan PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wan
authors.affiliation: Wuhan Institute of Biological Products, Wuhan PR China.
authors.forename: Zejun
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Wuhan Institute of Biological Products, Wuhan PR China.
authors.forename: Shengli
authors.initials: S
authors.lastname: Meng
authors.affiliation: Wuhan Institute of Biological Products, Wuhan PR China. Electronic address: shenshuo@inopharm.com.
authors.forename: Shuo
authors.initials: S
authors.lastname: Shen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017250:Caliciviridae Infections / Q000821:virology; D002213:Capsid / Q000648:ultrastructure; D036022:Capsid Proteins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D008969:Molecular Sequence Data; D029322:Norovirus / Q000235:genetics / Q000648:ultrastructure*; D010316:Particle Size; D016415:Sequence Alignment; D017384:Sequence Deletion; D061987:Sf9 Cells; D018411:Spodoptera
chemical_list: D036022:Capsid Proteins
keywords: Norovirus; Smaller viral particles; Truncated capsid protein; VLPs