title: A new strategy for label-free detection of lymphoma cancer cells.
abstract: In this paper, a new strategy for highly selective and sensitive direct detection of lymphoma cells by exploiting the interaction between a peptide and its B-cell receptor, has been evaluated. In particular, an idiotype peptide, able to specifically bind the B-cell receptor of A20 cells in mice engrafted with A20 lymphoma, has been used as molecular probe. The new detection technique has been demonstrated on a planar crystalline silicon chip. Coverage of 85% of silicon surface and detection efficiency of 8.5 × 10(-3) cells/μm(2) were obtained. The recognition strategy promises to extend its application in studying the interaction between ligands and their cell-surface receptors.
authors.affiliation: Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Via S. Pansini 5, 80131 Naples, Italy.
authors.forename: Nicola M
authors.initials: NM
authors.lastname: Martucci
authors.affiliation: Institute for Microelectronics and Microsystems, National Council of Research, Via P. Castellino 111, 80131 Naples, Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Rea
authors.affiliation: Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Via S. Pansini 5, 80131 Naples, Italy.
authors.forename: Immacolata
authors.initials: I
authors.lastname: Ruggiero
authors.affiliation: Institute for Microelectronics and Microsystems, National Council of Research, Via P. Castellino 111, 80131 Naples, Italy ; Department of Pharmacy, University of Naples Federico II, Via Domenico Montesano 49, 80131 Naples, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Terracciano
authors.affiliation: Institute for Microelectronics and Microsystems, National Council of Research, Via P. Castellino 111, 80131 Naples, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: De Stefano
authors.affiliation: Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Via S. Pansini 5, 80131 Naples, Italy.
authors.forename: Nunzia
authors.initials: N
authors.lastname: Migliaccio
authors.affiliation: Department of Experimental and Clinical Medicine, University "Magna Graecia" of Catanzaro, Viale Europa, 88100 Germaneto, Catanzaro, Italy.
authors.forename: Camillo
authors.initials: C
authors.lastname: Palmieri
authors.affiliation: Department of Experimental and Clinical Medicine, University "Magna Graecia" of Catanzaro, Viale Europa, 88100 Germaneto, Catanzaro, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Scala
authors.affiliation: Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Via S. Pansini 5, 80131 Naples, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Arcari
authors.affiliation: Institute for Microelectronics and Microsystems, National Council of Research, Via P. Castellino 111, 80131 Naples, Italy.
authors.forename: Ivo
authors.initials: I
authors.lastname: Rendina
authors.affiliation: Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Via S. Pansini 5, 80131 Naples, Italy.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Lamberti
keywords: (000.1430) Biology and medicine; (280.1415) Biological sensing and sensors