title: mFISH analysis of irradiated human fibroblasts: a comparison among radiations with different quality in the low-dose range.
abstract: The present investigation aimed to characterise the shape of dose-response curve and determining the frequency distribution of various aberration types as a function of dose and radiation quality in AG01522 primary human fibroblasts in the 0.1- to 1-Gy dose range. For this purpose, the cells were irradiated with 7.7 and 28.5 keV µm(-1) low-energy protons, 62 keV µm(-1 4)He(2+) ions (LNL Radiobiology facility) or X rays and samples collected for 24-colour mFISH analysis. X rays and 7.7 keV µm(-1) protons displayed a quadratic dose-response curve solely for total and simple exchanges, whereas for high-linear energy transfer radiations, a linear dose-response curve was observed for all the aberration categories, with the exception of complex exchanges.
authors.affiliation: Dip. di Scienze, Università Roma Tre, Roma, Italia INFN-Sezione di Roma Tre, Roma, Italia.
authors.forename: F
authors.initials: F
authors.lastname: Berardinelli
authors.affiliation: Dip. di Scienze, Università Roma Tre, Roma, Italia INFN-Sezione di Roma Tre, Roma, Italia.
authors.forename: D
authors.initials: D
authors.lastname: Nieri
authors.affiliation: Dip. di Scienze, Università Roma Tre, Roma, Italia INFN-Sezione di Roma Tre, Roma, Italia.
authors.forename: C
authors.initials: C
authors.lastname: Tanzarella
authors.affiliation: INFN-Laboratori Nazionali di Legnaro (LNL), Legnaro, Padova, Italia.
authors.forename: R
authors.initials: R
authors.lastname: Cherubini
authors.affiliation: INFN-Laboratori Nazionali di Legnaro (LNL), Legnaro, Padova, Italia.
authors.forename: V
authors.initials: V
authors.lastname: De Nadal
authors.affiliation: INFN-Laboratori Nazionali di Legnaro (LNL), Legnaro, Padova, Italia.
authors.forename: S
authors.initials: S
authors.lastname: Gerardi
authors.affiliation: Dip. di Scienze, Università Roma Tre, Roma, Italia INFN-Sezione di Roma Tre, Roma, Italia.
authors.forename: A
authors.initials: A
authors.lastname: Sgura
authors.affiliation: Dip. di Scienze, Università Roma Tre, Roma, Italia INFN-Sezione di Roma Tre, Roma, Italia antonio.antoccia@uniroma3.it.
authors.forename: A
authors.initials: A
authors.lastname: Antoccia
mesh_terms: D002869:Chromosome Aberrations / Q000528:radiation effects*; D005347:Fibroblasts / Q000528:radiation effects*; D020450:Heavy Ions; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D018499:Linear Energy Transfer / Q000528:radiation effects*; D011522:Protons; D011829:Radiation Dosage; D014965:X-Rays
chemical_list: D011522:Protons