title: High-molecular-weight glutenin subunit-deficient mutants induced by ion beam and the effects of Glu-1 loci deletion on wheat quality properties.
abstract: BACKGROUND
  High-molecular-weight glutenin subunits (HMW-GSs) play a critical role in determining the viscoelastic properties of wheat. Mutations induced by ion beam radiation have been applied to improve the yield and quality of crop. In this study, HMW-GS-deficient mutant lines were selected and the effects of Glu-1 loci deletion on wheat quality properties were illustrated according to the analysis of dry seeds of common wheat (Triticum aestivum L.) Xiaoyan 81 treated with a nitrogen ion beam.
  
  
  RESULTS
  Three HMW-GS-deficient mutant lines were obtained and then detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Large-chromosome-fragment deletion resulted in specific deficiencies, and the deleted region sizes were determined using molecular markers. Agronomic characters, quantity and proportion of glutenins and dough microstructure of the deletion lines all proved to be quite different from those of wild-type Xiaoyan 81. Analysis of quality properties suggested that GluA1(-) had superior property parameters, while GluB1(-) and GluD1(-) both showed a significant decrease in quality properties compared with Xiaoyan 81.
  
  
  CONCLUSIONS
  The effects of the three Glu-1 loci on flour and dough quality-related parameters should be Glu-D1 > Glu-B1 > Glu-A1. Ion beam radiation can be used as a mutagen to create new crop mutants.
authors.affiliation: Henan Key Lab of Ion Beam Bioengineering, Zhengzhou University, Zhengzhou, 450052, Henan, China.|State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Lujun
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Henan Key Lab of Ion Beam Bioengineering, Zhengzhou University, Zhengzhou, 450052, Henan, China.
authors.forename: Qiufang
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Henan Key Lab of Ion Beam Bioengineering, Zhengzhou University, Zhengzhou, 450052, Henan, China.
authors.forename: Mingjie
authors.initials: M
authors.lastname: Su
authors.affiliation: Minsheng College, Henan University, Kaifeng, 450001,Henan, China.
authors.forename: Biao
authors.initials: B
authors.lastname: Yan
authors.affiliation: State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
authors.forename: Xiangqi
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Henan Key Lab of Ion Beam Bioengineering, Zhengzhou University, Zhengzhou, 450052, Henan, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Jiao
mesh_terms: D001939:Bread / Q000032:analysis; D018744:DNA, Plant / Q000032:analysis; D004548:Elasticity; D004591:Electrophoresis, Polyacrylamide Gel; D005433:Flour / Q000032:analysis; D017353:Gene Deletion; D017343:Genes, Plant; D056426:Genetic Loci / Q000235:genetics*; D005983:Glutens / Q000737:chemistry* / Q000235:genetics* / Q000502:physiology; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D016133:Polymerase Chain Reaction; D021122:Protein Subunits / Q000032:analysis / Q000737:chemistry / Q000502:physiology; D012639:Seeds / Q000737:chemistry / Q000235:genetics; D014908:Triticum / Q000737:chemistry* / Q000235:genetics*; D014783:Viscosity
chemical_list: D018744:DNA, Plant; D021122:Protein Subunits; D005983:Glutens; C037302:glutenin
keywords: Glu-1 loci deletion lines; HMW-GSs; ion beam; quality properties