title: Immunomodulatory activity of ketamine in human astroglial A172 cells: Possible relevance to its rapid antidepressant activity.
abstract: To determine if the immunomodulatory effect of ketamine is relevant to its rapid antidepressant activity, cultured human astroglial cells were incubated with ketamine, cytokine mix, or both. At 24h, ketamine dose-dependently (100-500 μM) decreased IL-6 and TNFα production and gene expression and, at clinically relevant concentration (100 μM), augmented IL-β release and gene expression in both unstimulated and cytokine-stimulated cells. In unstimulated cells, ketamine also increased IL-8 production and mRNA expression. The reduction in IL-6 mRNA was significant within 1h in unstimulated cells and at 4h after stimulation. Ketamine suppressed the production of the only established depression-relevant proinflammatory cytokines, IL-6 and TNFα.
authors.affiliation: Laboratory of Pediatric Infectious Diseases, Felsenstein Medical Research Center, Petach Tikva, Israel; Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel. Electronic address: Yuhas@post.tau.ac.il.
authors.forename: Yael
authors.initials: Y
authors.lastname: Yuhas
authors.affiliation: Laboratory of Pediatric Infectious Diseases, Felsenstein Medical Research Center, Petach Tikva, Israel; Department of Pediatrics A, Schneider Children's Medical Center of Israel, Petach Tikva, Israel; Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Shai
authors.initials: S
authors.lastname: Ashkenazi
authors.affiliation: Laboratory of Pediatric Infectious Diseases, Felsenstein Medical Research Center, Petach Tikva, Israel.
authors.forename: Eva
authors.initials: E
authors.lastname: Berent
authors.affiliation: Laboratory of Biological Psychiatry, Felsenstein Medical Research Center, Petach Tikva, Israel; Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel; Research Unit, Geha Mental Health Center, Petach Tikva, Israel.
authors.forename: Abraham
authors.initials: A
authors.lastname: Weizman
mesh_terms: D001253:Astrocytes / Q000187:drug effects*; D045744:Cell Line, Tumor; D016207:Cytokines / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D005909:Glioblastoma / Q000473:pathology; D006801:Humans; D007649:Ketamine / Q000494:pharmacology*; D040081:Protein Array Analysis
chemical_list: D016207:Cytokines; D018691:Excitatory Amino Acid Antagonists; D007649:Ketamine
keywords: Antidepressant; Astroglial cells; Cytokines; Immunomodulation; Ketamine