title: The Ty1 LTR-retrotransposon of budding yeast,Saccharomyces cerevisiae.
abstract: Long-terminal repeat (LTR)-retrotransposons generate a copy of their DNA (cDNA) by reverse transcription of their RNA genome in cytoplasmic nucleocapsids. They are widespread in the eukaryotic kingdom and are the evolutionary progenitors of retroviruses [1]. The Ty1 element of the budding yeastSaccharomyces cerevisiaewas the first LTR-retrotransposon demonstrated to mobilize through an RNA intermediate, and not surprisingly, is the best studied. The depth of our knowledge of Ty1 biology stems not only from the predominance of active Ty1 elements in theS. cerevisiaegenome but also the ease and breadth of genomic, biochemical and cell biology approaches available to study cellular processes in yeast. This review describes the basic structure of Ty1 and its gene products, the replication cycle, the rapidly expanding compendium of host co-factors known to influence retrotransposition and the nature of Ty1's elaborate symbiosis with its host. Our goal is to illuminate the value of Ty1 as a paradigm to explore the biology of LTR-retrotransposons in multicellular organisms, where the low frequency of retrotransposition events presents a formidable barrier to investigations of retrotransposon biology.
authors.affiliation: Laboratory of Molecular Genetics, Wadsworth Center, and Department of Biomedical Sciences, University at Albany-SUNY; Center for Medical Sciences, 150 New Scotland Avenue, Albany, NY, 12208, USA.
authors.forename: M Joan
authors.initials: MJ
authors.lastname: Curcio
authors.affiliation: Laboratory of Molecular Genetics, Wadsworth Center, and Department of Biomedical Sciences, University at Albany-SUNY; Center for Medical Sciences, 150 New Scotland Avenue, Albany, NY, 12208, USA.
authors.forename: Sheila
authors.initials: S
authors.lastname: Lutz
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, Institut Universitaire d'Hématologie, Hôpital St. Louis, Institut National de la Santé et de la Recherche Médicale (INSERM) UMR 944, Centre National de la Recherche Scientifique (CNRS) UMR 7212, 1 Avenue Claude Vellefaux, F-75475 Paris cedex 10, FRANCE.
authors.forename: Pascale
authors.initials: P
authors.lastname: Lesage