title: Effects of Bu Shen Yi Sui Capsule on Th17/Treg cytokines in C57BL/6 mice with experimental autoimmune encephalomyelitis.
abstract: BACKGROUND
  T helper (Th) 17 and regulatory T (Treg) cells play a critical role in the pathogenesis of multiple sclerosis (MS) disease. Bu Shen Yi Sui Capsule (BSYSC), a traditional Chinese medicine formula, has been used clinically for the treatment of MS patients in China.
  
  
  METHODS
  To evaluate the neuroprotective effects and the underlying mechanisms of BSYSC on MS, experimental autoimmune encephalomyelitis (EAE) model in C57BL/6 mice was induced with myelin oligodendrocyte glycoprotein (MOG) 35-55. Th17 and Treg cells and the related cytokines were detected by flow cytometry, ELISA, real-time quantitative reverse transcription PCR, western blot and immunohistochemistry.
  
  
  RESULTS
  We found that BSYSC improved neurological function, reduced inflammatory cell infiltration and damage to the axons and myelin in the brain and spinal cord. BSYSC down-regulated markedly the ratio of CD4 + IL-17+/CD4 + CD25 + FoxP3+ T cells in the spleen, decreased the cytokines of IL-17A, IL-6, IL-23, TGF-beta1 in the brain, and dropped the ratio of IL-17A and FoxP3 mRNA and protein in the brain or spinal cord at different stages.
  
  
  CONCLUSIONS
  The study demonstrated that BSYSC had a strong neuroprotective effect on EAE mice. The protective mechanisms of BSYSC might be associated with mediating the regulation of Th17/Treg cells.
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing, 100069, PR China. fiona19880607@sina.cn.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zheng
authors.affiliation: Beijing Tian Tan Hospital, Capital Medical University, Beijing, 100050, PR China. yangtaosun2001@163.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Yang
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing, 100069, PR China. fangling0113@163.com.
authors.forename: Ling
authors.initials: L
authors.lastname: Fang
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing, 100069, PR China. 15652523423@163.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing, 100069, PR China. 793146134@qq.com.
authors.forename: Haolong
authors.initials: H
authors.lastname: Liu
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing, 100069, PR China. zhaohui8957@sina.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Beijing Institute for Drug Control, Beijing, 100035, PR China. zhaoyiyi410@163.com.
authors.forename: Yiyi
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Beijing Institute for Drug Control, Beijing, 100035, PR China. guohz@bidc.org.cn.
authors.forename: Hongzhu
authors.initials: H
authors.lastname: Guo
authors.affiliation: Beijing Tian Tan Hospital, Capital Medical University, Beijing, 100050, PR China. yongpingf@hotmail.com.
authors.forename: Yongping
authors.initials: Y
authors.lastname: Fan
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing, 100069, PR China. tmwangl@ccmu.edu.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D002681:China; D016207:Cytokines / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000188:drug therapy* / Q000276:immunology / Q000378:metabolism; D005260:Female; D051858:Forkhead Transcription Factors / Q000378:metabolism; D006801:Humans; D007378:Interleukins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009103:Multiple Sclerosis / Q000188:drug therapy* / Q000276:immunology / Q000378:metabolism; D063308:Myelin-Oligodendrocyte Glycoprotein; D018696:Neuroprotective Agents / Q000494:pharmacology / Q000627:therapeutic use; D008517:Phytotherapy; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism; D013154:Spleen / Q000187:drug effects / Q000378:metabolism; D050378:T-Lymphocytes, Regulatory / Q000378:metabolism*; D058504:Th17 Cells / Q000378:metabolism*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: D016207:Cytokines; D004365:Drugs, Chinese Herbal; D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D007378:Interleukins; D063308:Myelin-Oligodendrocyte Glycoprotein; D018696:Neuroprotective Agents; D053773:Transforming Growth Factor beta1; C527186:bushen yisui