title: The potential of follicle-stimulating hormone peptide-modified triptolide-loaded nanoparticles to induce a mouse model of premature ovarian insufficiency.
abstract: The use of triptolide (TP) is limited by its poor water solubility and severe toxicity. In this study, we developed an active drug delivery system (TP-loaded nanoparticles) that could help improve the water solubility of TP and decrease its toxicity. Then, we investigated whether TP-loaded nanoparticles could be used to establish a novel premature ovarian insufficiency mouse model. The mice treated with TP-loaded nanoparticles for 35 days displayed normal growth, decreased serum antimullerian hormone, prominent ovarian fibrosis and vacuolar changes, fewer follicles and corpus lutea, increased collapsed oocytes and follicle apoptosis, and sterility. In conclusion, this model appears to show the reproductive characteristics associated with premature ovarian insufficiency in women and will allow us to study the mechanism of the effects of traditional Chinese medicine on gonadal toxicity.
authors.affiliation: Obstetrics and Gynecology Hospital, Fudan University, Shanghai, People's Republic of China ; Department of Obstetrics and Gynecology of Shanghai Medical College, Fudan University, Shanghai, People's Republic of China ; Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Fudan University, Shanghai, People's Republic of China.
authors.forename: Xiu-Ying
authors.initials: XY
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai, People's Republic of China.
authors.forename: Wu-Lian
authors.initials: WL
authors.lastname: Chen
authors.affiliation: Obstetrics and Gynecology Hospital, Fudan University, Shanghai, People's Republic of China ; Department of Obstetrics and Gynecology of Shanghai Medical College, Fudan University, Shanghai, People's Republic of China ; Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Fudan University, Shanghai, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Ma
authors.affiliation: Obstetrics and Gynecology Hospital, Fudan University, Shanghai, People's Republic of China ; Department of Obstetrics and Gynecology of Shanghai Medical College, Fudan University, Shanghai, People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Gu
authors.affiliation: Obstetrics and Gynecology Hospital, Fudan University, Shanghai, People's Republic of China ; Department of Obstetrics and Gynecology of Shanghai Medical College, Fudan University, Shanghai, People's Republic of China.
authors.forename: Xi-Rong
authors.initials: XR
authors.lastname: Xiao
authors.affiliation: Obstetrics and Gynecology Hospital, Fudan University, Shanghai, People's Republic of China ; Department of Obstetrics and Gynecology of Shanghai Medical College, Fudan University, Shanghai, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D004195:Disease Models, Animal; D004224:Diterpenes / Q000494:pharmacology*; D016503:Drug Delivery Systems; D004852:Epoxy Compounds / Q000494:pharmacology; D005260:Female; D005298:Fertility / Q000187:drug effects; D005640:Follicle Stimulating Hormone / Q000737:chemistry*; D020287:In Situ Nick-End Labeling; D051379:Mice; D008810:Mice, Inbred C57BL; D053758:Nanoparticles / Q000737:chemistry*; D010616:Phenanthrenes / Q000494:pharmacology*; D016649:Primary Ovarian Insufficiency / Q000188:drug therapy* / Q000473:pathology
chemical_list: D004224:Diterpenes; D004852:Epoxy Compounds; D010616:Phenanthrenes; C001899:triptolide; D005640:Follicle Stimulating Hormone
keywords: animal model; drug delivery; nanoparticles; peptide; premature ovarian insufficiency