title: [Detection of Staphylococcus aureus toxins using immuno-PCR].
abstract: A highly sensitive test system, based on the method of immuno-PCR, was developed for the detection of two staphylococcal toxins: enterotoxin A (SEA) and toxic shock syndrome toxin (TSST). A key element of the developed systems was obtaining supramolecular complexes of bisbiotinylated oligodeoxynucleotides and streptavidin, which were used as DNA tags. Specificity studies showed no cross-reactivity when determining SEA and TSST. The sensitivity of detection of these toxins in the culture supernatants S. aureus was not less than 10 pg/mL.
authors.forename: A V
authors.initials: AV
authors.lastname: Maerle
authors.forename: D Iu
authors.initials: DIu
authors.lastname: Riazantsev
authors.forename: O A
authors.initials: OA
authors.lastname: Dmitrenko
authors.forename: E Ia
authors.initials: EIa
authors.lastname: Petrova
authors.forename: R L
authors.initials: RL
authors.lastname: Komaleva
authors.forename: I V
authors.initials: IV
authors.lastname: Sergeev
authors.forename: D Iu
authors.initials: DIu
authors.lastname: Trofimov
authors.forename: S K
authors.initials: SK
authors.lastname: Zavriev
mesh_terms: D001427:Bacterial Toxins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004768:Enterotoxins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012772:Shock, Septic / Q000175:diagnosis / Q000382:microbiology; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000737:chemistry / Q000235:genetics / Q000472:pathogenicity; D018089:Superantigens / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004768:Enterotoxins; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal; C026066:enterotoxin A, Staphylococcal