title: Regulator of G protein signaling 1 suppresses CXCL12-mediated migration and AKT activation in RPMI 8226 human plasmacytoma cells and plasmablasts.
abstract: Migration of plasma cells to the bone marrow is critical factor to humoral immunity and controlled by chemokines. Regulator of G protein signaling 1 (RGS1) is a GTPase-activating protein that controls various crucial functions such as migration. Here, we show that RGS1 controls the chemotactic migration of RPMI 8226 human plasmacytoma cells and human plasmablasts. LPS strongly increased RGS1 expression and retarded the migration of RPMI 8226 cells by suppressing CXCL12-mediated AKT activation. RGS1 knockdown by siRNA abolished the retardation of migration and AKT suppression by LPS. RGS1-dependent regulation of migration via AKT is also observed in cultured plasmablasts. We propose novel functions of RGS1 that suppress AKT activation and the migration of RPMI 8226 cells and plasmablasts in CXCL12-mediated chemotaxis.
authors.affiliation: Cell Dysfunction Research Center, University of Ulsan College of Medicine, Seoul, Korea; Department of Pathology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.
authors.forename: Hyo-Kyung
authors.initials: HK
authors.lastname: Pak
authors.affiliation: Cell Dysfunction Research Center, University of Ulsan College of Medicine, Seoul, Korea.
authors.forename: Minchan
authors.initials: M
authors.lastname: Gil
authors.affiliation: Cell Dysfunction Research Center, University of Ulsan College of Medicine, Seoul, Korea.
authors.forename: Yoonkyung
authors.initials: Y
authors.lastname: Lee
authors.affiliation: Cell Dysfunction Research Center, University of Ulsan College of Medicine, Seoul, Korea.
authors.forename: Hyunji
authors.initials: H
authors.lastname: Lee
authors.affiliation: Cell Dysfunction Research Center, University of Ulsan College of Medicine, Seoul, Korea.
authors.forename: A-Neum
authors.initials: AN
authors.lastname: Lee
authors.affiliation: Department of Pathology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.
authors.forename: Jin
authors.initials: J
authors.lastname: Roh
authors.affiliation: Cell Dysfunction Research Center, University of Ulsan College of Medicine, Seoul, Korea; Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea; Department of Pathology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.
authors.forename: Chan-Sik
authors.initials: CS
authors.lastname: Park
mesh_terms: D054377:Chemokine CXCL12 / Q000235:genetics* / Q000378:metabolism; D002633:Chemotaxis / Q000187:drug effects; D005786:Gene Expression Regulation; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D010950:Plasma Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D010954:Plasmacytoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics* / Q000378:metabolism; D020710:RGS Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014407:Tumor Cells, Cultured
chemical_list: C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D008070:Lipopolysaccharides; D020710:RGS Proteins; C080831:RGS1 protein, human; D034741:RNA, Small Interfering; D051057:Proto-Oncogene Proteins c-akt