title: Molecular identification of Coccidioides immitis in formalin-fixed, paraffin-embedded (FFPE) tissues from a Colombian patient.
abstract: Coccidioides immitis and C. posadasii are the etiologic agents of coccidioidomycosis, an endemic fungal disease of the Americas. In Colombia, this mycosis is uncommon, and only five cases, two of them imported, have been documented.By means of DNA sequencing, C. immitis was identified in formalin-fixed, paraffin-embedded archival tissues samples from the 5th Colombian patient diagnosed in 1997. The patient was born in Pinto, Department of Magdalena, and had never visited other geographic regions, a reason to consider that the mycosis had been acquired locally.This species is primarily found in California although it has been occasionally reported in other geographic areas such as Mexico and Brazil. This is the first indigenous report of C. immitis-associated coccidioidomycosis in a Colombian patient.
authors.affiliation: Departamento de Micología. Instituto Nacional de Enfermedades Infecciosas INEI-ANLIS "Dr. Carlos G. Malbrán", Buenos Aires, Argentina.
authors.forename: Cristina E
authors.initials: CE
authors.lastname: Canteros
authors.affiliation: Departamento de Patología, Hospital Pablo Tobón Uribe, Medellín, Colombia Laboratorios Dinámica, Institución Prestadora de Servicios (IPS), Medellín, Colombia Universidad Pontificia Bolivariana, Escuela de Ciencias de la Salud, Medellín, Colombia.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Vélez H
authors.affiliation: Departamento de Micología. Instituto Nacional de Enfermedades Infecciosas INEI-ANLIS "Dr. Carlos G. Malbrán", Buenos Aires, Argentina.
authors.forename: Adriana I
authors.initials: AI
authors.lastname: Toranzo
authors.affiliation: Departamento de Micología. Instituto Nacional de Enfermedades Infecciosas INEI-ANLIS "Dr. Carlos G. Malbrán", Buenos Aires, Argentina.
authors.forename: Roberto
authors.initials: R
authors.lastname: Suárez-Alvarez
authors.affiliation: Corporación para Investigaciones Biológicas (CIB), Medellín, Colombia Hospital La María, Medellín, Colombia.
authors.forename: Ángela
authors.initials: Á
authors.lastname: Tobón O
authors.affiliation: Corporación para Investigaciones Biológicas (CIB), Medellín, Colombia Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia.
authors.forename: María del Pilar
authors.initials: Mdel P
authors.lastname: Jimenez A
authors.affiliation: Corporación para Investigaciones Biológicas (CIB), Medellín, Colombia angelares@une.net.co.
authors.forename: Ángela
authors.initials: Á
authors.lastname: Restrepo M
mesh_terms: D001706:Biopsy; D003045:Coccidioides / Q000235:genetics / Q000302:isolation & purification*; D003047:Coccidioidomycosis / Q000175:diagnosis* / Q000382:microbiology / Q000473:pathology; D003105:Colombia; D005557:Formaldehyde; D006801:Humans; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques; D010232:Paraffin; D057089:Pathology, Molecular; D010361:Patients; D016707:Tissue Fixation
chemical_list: D005557:Formaldehyde; D010232:Paraffin
keywords: Coccidioides immitis; Coccidioidomycosis; Colombia; indigenous; molecular identification