title: Intracellular Concentrations of Borrelia burgdorferi Cyclic Di-AMP Are Not Changed by Altered Expression of the CdaA Synthase.
abstract: The second messenger nucleotide cyclic diadenylate monophosphate (c-di-AMP) has been identified in several species of Gram positive bacteria and Chlamydia trachomatis. This molecule has been associated with bacterial cell division, cell wall biosynthesis and phosphate metabolism, and with induction of type I interferon responses by host cells. We demonstrate that B. burgdorferi produces a c-di-AMP synthase, which we designated CdaA. Both CdaA and c-di-AMP levels are very low in cultured B. burgdorferi, and no conditions were identified under which cdaA mRNA was differentially expressed. A mutant B. burgdorferi was produced that expresses high levels of CdaA, yet steady state borrelial c-di-AMP levels did not change, apparently due to degradation by the native DhhP phosphodiesterase. The function(s) of c-di-AMP in the Lyme disease spirochete remains enigmatic.
authors.affiliation: Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, Lexington, Kentucky, United States of America.
authors.forename: Christina R
authors.initials: CR
authors.lastname: Savage
authors.affiliation: Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, Lexington, Kentucky, United States of America.
authors.forename: William K
authors.initials: WK
authors.lastname: Arnold
authors.affiliation: Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, Lexington, Kentucky, United States of America.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Gjevre-Nail
authors.affiliation: Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, United States of America.
authors.forename: Benjamin J
authors.initials: BJ
authors.lastname: Koestler
authors.affiliation: Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, United States of America.
authors.forename: Eric L
authors.initials: EL
authors.lastname: Bruger
authors.affiliation: Department of Molecular Genetics and Microbiology, Center for Microbial Pathogenesis, Duke University, Durham, North Carolina, United States of America.
authors.forename: Jeffrey R
authors.initials: JR
authors.lastname: Barker
authors.affiliation: Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, United States of America.
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Waters
authors.affiliation: Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, Lexington, Kentucky, United States of America.
authors.forename: Brian
authors.initials: B
authors.lastname: Stevenson
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D025065:Borrelia burgdorferi / Q000201:enzymology* / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D040901:Proteomics / Q000379:methods
chemical_list: D001426:Bacterial Proteins; D000242:Cyclic AMP; D010727:Phosphoric Diester Hydrolases