title: Tissue Engineering Strategies as Tools for Personalized Meningioma Treatment.
abstract: Pharmacogenomics, the science of how genetic makeup influences an individual's reaction to drugs, is an innovative tool for providing critical insights into how a patient will respond to a particular treatment. In the present work, we constructed cancer-like tissues to be used as tools for determining the most effective drug for an individual patient. Using tissue engineering strategies, we generated two different solid tumor-like tissues in vitro, a neuronal tumor (meningioma) and a nonmelanoma skin cancer. Samples were tested by both histological and genetic approaches (using a comparative genomic hybridization array, and the relative World Health Organization classification of the samples was compared with the results obtained by the molecular analyses. Our data confirmed the ability of the cells to maintain their phenotype in three-dimensional scaffolds as well as the strong relationship between chromosomal alterations and histological malignancy grades. We then validated the in vitro construction of tumor-like tissues as a potential tool for developing personalized drug treatments.
authors.affiliation: Department of Biomedical Sciences, University of Padova, Padova, Italy.
authors.forename: Letizia
authors.initials: L
authors.lastname: Ferroni
authors.affiliation: Department of Neurosciences, University of Padova, Padova, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Della Puppa
authors.affiliation: Department of Neurosciences, University of Padova, Padova, Italy.
authors.forename: Domenico
authors.initials: D
authors.lastname: D'Avella
authors.affiliation: Department of Animal Medicine, Production and Health (MAPS), University of Padova, Padova, Italy.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Isola
authors.affiliation: Department of Neurosciences, University of Padova, Padova, Italy.
authors.forename: Renato
authors.initials: R
authors.lastname: Scienza
authors.affiliation: Department of Biomedical Sciences, University of Padova, Padova, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Gardin
authors.affiliation: Department of Biomedical Sciences, University of Padova, Padova, Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Zavan
mesh_terms: D002460:Cell Line; D002478:Cells, Cultured; D055028:Comparative Genomic Hybridization; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry; D008577:Meningeal Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D008579:Meningioma / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D057285:Precision Medicine / Q000379:methods*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry; D014407:Tumor Cells, Cultured
chemical_list: D006820:Hyaluronic Acid
keywords: Biomaterial; Drug screening; Personalized medicine; Solid cancer; Tissue engineering