title: Detection of quantitative trait loci for mineral content of Nelore longissimus dorsi muscle.
abstract: BACKGROUND
  Beef cattle require dietary minerals for optimal health, production and reproduction. Concentrations of minerals in tissues are at least partly genetically determined. Mapping genomic regions that affect the mineral content of bovine longissimus dorsi muscle can contribute to the identification of genes that control mineral balance, transportation, absorption and excretion and that could be associated to metabolic disorders.
  
  
  METHODS
  We applied a genome-wide association strategy and genotyped 373 Nelore steers from 34 half-sib families with the Illumina BovineHD BeadChip. Genome-wide association analysis was performed for mineral content of longissimus dorsi muscle using a Bayesian approach implemented in the GenSel software.
  
  
  RESULTS
  Muscle mineral content in Bos indicus cattle was moderately heritable, with estimates ranging from 0.29 to 0.36. Our results suggest that variation in mineral content is influenced by numerous small-effect QTL (quantitative trait loci) but a large-effect QTL that explained 6.5% of the additive genetic variance in iron content was detected at 72 Mb on bovine chromosome 12. Most of the candidate genes present in the QTL regions for mineral content were involved in signal transduction, signaling pathways via integral (also called intrinsic) membrane proteins, transcription regulation or metal ion binding.
  
  
  CONCLUSIONS
  This study identified QTL and candidate genes that affect the mineral content of skeletal muscle. Our findings provide the first step towards understanding the molecular basis of mineral balance in bovine muscle and can also serve as a basis for the study of mineral balance in other organisms.
authors.affiliation: Department of Genetics and Evolution, Federal University of Sao Carlos, São Carlos, SP, Brazil. ptizioto@gmail.com.|Division of Animal Sciences, University of Missouri, Columbia, MO, USA. ptizioto@gmail.com.
authors.forename: Polyana C
authors.initials: PC
authors.lastname: Tizioto
authors.affiliation: Division of Animal Sciences, University of Missouri, Columbia, MO, USA. taylorjerr@missouri.edu.
authors.forename: Jeremy F
authors.initials: JF
authors.lastname: Taylor
authors.affiliation: Division of Animal Sciences, University of Missouri, Columbia, MO, USA. DeckerJE@missouri.edu.
authors.forename: Jared E
authors.initials: JE
authors.lastname: Decker
authors.affiliation: Federal Institute of Education, Bahia Science and Technology, Valença, BA, Brazil. caioquimica@yahoo.com.br.
authors.forename: Caio F
authors.initials: CF
authors.lastname: Gromboni
authors.affiliation: Embrapa Southeast Livestock, São Carlos, SP, Brazil. mauricio.mudadu@embrapa.br.
authors.forename: Mauricio A
authors.initials: MA
authors.lastname: Mudadu
authors.affiliation: Division of Animal Sciences, University of Missouri, Columbia, MO, USA. schnabelr@missouri.edu.
authors.forename: Robert D
authors.initials: RD
authors.lastname: Schnabel
authors.affiliation: Department of Animal Science, University of São Paulo/ESALQ, Piracicaba, SP, Brazil. llcoutinho@usp.br.
authors.forename: Luiz L
authors.initials: LL
authors.lastname: Coutinho
authors.affiliation: Department of Animal Science, University of São Paulo/ESALQ, Piracicaba, SP, Brazil. gbmourao@usp.br.
authors.forename: Gerson B
authors.initials: GB
authors.lastname: Mourão
authors.affiliation: Department of Genetics and Evolution, Federal University of Sao Carlos, São Carlos, SP, Brazil. priscilaneuberndeoliveira@gmail.com.
authors.forename: Priscila S N
authors.initials: PS
authors.lastname: Oliveira
authors.affiliation: Department of Genetics and Evolution, Federal University of Sao Carlos, São Carlos, SP, Brazil. mm.rissato@gmail.com.
authors.forename: Marcela M
authors.initials: MM
authors.lastname: Souza
authors.affiliation: Department of Animal Science, Iowa State University, Ames, IA, USA. jreecy@iastate.edu.
authors.forename: James M
authors.initials: JM
authors.lastname: Reecy
authors.affiliation: Embrapa Southeast Livestock, São Carlos, SP, Brazil. renata.nassu@embrapa.br.
authors.forename: Renata T
authors.initials: RT
authors.lastname: Nassu
authors.affiliation: Embrapa Southeast Livestock, São Carlos, SP, Brazil. flavia.donatoni@embrapa.br.
authors.forename: Flavia A
authors.initials: FA
authors.lastname: Bressani
authors.affiliation: Embrapa Southeast Livestock, São Carlos, SP, Brazil. patricia.tholon@embrapa.br.
authors.forename: Patricia
authors.initials: P
authors.lastname: Tholon
authors.affiliation: United States Department of Agriculture (USDA), Agricultural Research Service, Beltsville, MD, USA. tad.sonstegard@ars.usda.gov.
authors.forename: Tad S
authors.initials: TS
authors.lastname: Sonstegard
authors.affiliation: Embrapa Southeast Livestock, São Carlos, SP, Brazil. mauricio.alencar@embrapa.br.
authors.forename: Mauricio M
authors.initials: MM
authors.lastname: Alencar
authors.affiliation: Embrapa Southeast Livestock, São Carlos, SP, Brazil. rymer.tullio@embrapa.br.
authors.forename: Rymer R
authors.initials: RR
authors.lastname: Tullio
authors.affiliation: Embrapa Southeast Livestock, São Carlos, SP, Brazil. ana.nogueira@embrapa.br.
authors.forename: Ana R A
authors.initials: AR
authors.lastname: Nogueira
authors.affiliation: Embrapa Southeast Livestock, São Carlos, SP, Brazil. luciana.regitano@embrapa.br.
authors.forename: Luciana C A
authors.initials: LC
authors.lastname: Regitano
mesh_terms: D000818:Animals; D001499:Bayes Theorem; D002417:Cattle / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D003198:Computer Simulation; D055106:Genome-Wide Association Study / Q000379:methods*; D023281:Genomics / Q000379:methods; D005838:Genotype; D008903:Minerals / Q000032:analysis* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000378:metabolism; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci
chemical_list: D008903:Minerals