title: Protective effect of artemisia asiatica extract against renal ischemia-reperfusion injury in mice.
abstract: OBJECTIVE
  An extract of Artemisia asiatica was reported to possess antioxidative and cytoprotective actions in various experiments. Ischemia-reperfusion injury remains a major problem in kidney transplant, and the inflammatory response to ischemia-reperfusion injury exacerbates the resultant renal injury. In the present study, we investigated whether an extract of Artemisia asiatica exhibits renoprotective effects against ischemia-reperfusion-induced acute kidney injury in mice.
  
  
  METHODS
  Renal ischemia-reperfusion injury was induced in male C57BL/6 mice by bilateral renal pedicle occlusion for 30 minutes followed by reperfusion for 48 hours. An extract of Artemisia asiatica (100 mg/kg oral) was administered 4 days before ischemia-reperfusion injury. Sham operation and phosphate-buffered saline were used as controls. Blood and renal tissues were evaluated at 48 hours after ischemiareperfusion injury.
  
  
  RESULTS
  Treatment with an extract of Artemisia asiatica significantly decreased blood urea nitrogen, serum creatinine levels, and kidney tubular injury (P ≤ .05). Western blot showed that an extract of Artemisia asiatica significantly increased the level of heme oxygenase-1 and B-cell lymphoma 2 at 48 hours after ischemia-reperfusion injury and attenuated the level of inducible nitric oxide synthase.
  
  
  CONCLUSIONS
  An extract of Artemisia asiatica improves acute renal ischemia-reperfusion injury by reducing inflammation and apoptosis. These findings suggest that an extract of Artemisia asiatica is a potential therapeutic agent against acute ischemia-induced renal damage.
authors.affiliation: From the Department of Surgery, University of Ulsan Gangneung Asan Hospital, Gangneung, Gangwon, South Korea.
authors.forename: Hyuk Jai
authors.initials: HJ
authors.lastname: Jang
authors.forename: Eui Kyun
authors.initials: EK
authors.lastname: Jeong
authors.forename: Seong Su
authors.initials: SS
authors.lastname: Kim
authors.forename: Ji Hwan
authors.initials: JH
authors.lastname: Lee
authors.forename: Mi Young
authors.initials: MY
authors.lastname: Oh
authors.forename: Ki Sung
authors.initials: KS
authors.lastname: Kang
authors.forename: Hak Cheol
authors.initials: HC
authors.lastname: Kwan
authors.forename: Kyung Il
authors.initials: KI
authors.lastname: Song
authors.forename: Dae Woon
authors.initials: DW
authors.lastname: Eom
authors.forename: Duck Jong
authors.initials: DJ
authors.lastname: Han
mesh_terms: D058186:Acute Kidney Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D000818:Animals; D019306:Artemisia; D015415:Biomarkers / Q000097:blood; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D019610:Cytoprotection; D004195:Disease Models, Animal; D051547:Heme Oxygenase-1 / Q000378:metabolism; D007668:Kidney / Q000098:blood supply / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D008810:Mice, Inbred C57BL; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal; D020011:Protective Agents / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D013997:Time Factors
chemical_list: D015415:Biomarkers; D008565:Membrane Proteins; D010936:Plant Extracts; D020011:Protective Agents; D019253:Proto-Oncogene Proteins c-bcl-2; C052617:Bcl2 protein, mouse; D003404:Creatinine; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; D051547:Heme Oxygenase-1; C496542:Hmox1 protein, mouse