title: Bioelectronic nose combined with a microfluidic system for the detection of gaseous trimethylamine.
abstract: A bioelectronic nose based on a novel microfluidic system (μBN) was fabricated to detect gaseous trimethylamine (TMA) in real-time. Single-walled carbon nanotube-field effect transistors (SWNT-FETs) were functionalized with olfactory receptor-derived peptides (ORPs) that can recognize the TMA molecules. The ORP-coated SWNT-FETs were assembled with a microfluidic channel and were sealed with top and bottom frames. This simple process was used to complete the μBNs, and a well-defined condition was achieved to detect the gaseous molecules. The μBNs allowed us to detect gaseous TMA molecules down to 10 parts per trillion (ppt) in real-time and showed high selectivity when distinguishing gaseous TMA from other gaseous odorants. The sensor was used to determine the quality of seafood (oysters), and spoiled seafood and other types of spoiled foods were also successfully discriminated without any pretreatment processes. These results indicate that portable-scale platforms can be manufactured by using μBNs and can be applicable for real-time on-site gas analysis.
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Seung Hwan
authors.initials: SH
authors.lastname: Lee
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Jong Hyun
authors.initials: JH
authors.lastname: Lim
authors.affiliation: Department of Physics and Astronomy, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Juhun
authors.initials: J
authors.lastname: Park
authors.affiliation: Department of Physics and Astronomy, Seoul National University, Seoul 151-742, Republic of Korea; Department of Biophysics and Chemical Biology, Seoul National University, Seoul 151-742, Republic of Korea. Electronic address: seunghun@snu.ac.kr.
authors.forename: Seunghun
authors.initials: S
authors.lastname: Hong
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, Republic of Korea; Bio-MAX Institute, Seoul National University, Seoul 151-742, Republic of Korea; Advanced Institutes of Convergence Technology, Suwon, Gyeonggi-do 443-270, Republic of Korea. Electronic address: thpark@snu.ac.kr.
authors.forename: Tai Hyun
authors.initials: TH
authors.lastname: Park
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D005504:Food Analysis / Q000295:instrumentation*; D063427:Food Quality; D005740:Gases / Q000032:analysis* / Q000378:metabolism; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D057230:Limit of Detection; D008744:Methylamines / Q000032:analysis* / Q000378:metabolism; D044085:Microfluidics / Q000295:instrumentation*; D008969:Molecular Sequence Data; D037742:Nanotubes, Carbon / Q000737:chemistry; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D018035:Receptors, Odorant / Q000737:chemistry / Q000378:metabolism; D017747:Seafood / Q000032:analysis*; D014173:Transistors, Electronic
chemical_list: D005740:Gases; D055767:Immobilized Proteins; D008744:Methylamines; D037742:Nanotubes, Carbon; D010455:Peptides; D018035:Receptors, Odorant; C023336:trimethylamine
keywords: Bioelectronic nose; Carbon nanotube; Microfluidic gas sensor; Olfactory receptor; Trimethylamine