title: Mechanical and biological properties of the micro-/nano-grain functionally graded hydroxyapatite bioceramics for bone tissue engineering.
abstract: Functionally graded materials (FGM) open the promising approach for bone tissue repair. In this study, a novel functionally graded hydroxyapatite (HA) bioceramic with micrograin and nanograin structure was fabricated. Its mechanical properties were tailored by composition of micrograin and nanograin. The dynamic mechanical analysis (DMA) indicated that the graded HA ceramics had similar mechanical property compared to natural bones. Their cytocompatibility was evaluated via fluorescent microscopy and MTT colorimetric assay. The viability and proliferation of rabbit bone marrow mesenchymal stem cells (BMSCs) on ceramics indicated that this functionally graded HA ceramic had better cytocompatibility than conventional HA ceramic. This study demonstrated that functionally graded HA ceramics create suitable structures to satisfy both the mechanical and biological requirements of bone tissues.
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China. Electronic address: changchunzhou@scu.edu.cn.
authors.forename: Changchun
authors.initials: C
authors.lastname: Zhou
authors.affiliation: School of Manufacturing Science and Engineering, Sichuan University, Chengdu 610065, China.
authors.forename: Congying
authors.initials: C
authors.lastname: Deng
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China.
authors.forename: Xuening
authors.initials: X
authors.lastname: Chen
authors.affiliation: School of Manufacturing Science and Engineering, Sichuan University, Chengdu 610065, China.
authors.forename: Xiufen
authors.initials: X
authors.lastname: Zhao
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China.
authors.forename: Yujiang
authors.initials: Y
authors.lastname: Fan
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China. Electronic address: zhangxd@scu.edu.cn.
authors.forename: Xingdong
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001842:Bone and Bones; D049109:Cell Proliferation; D002516:Ceramics / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D017886:Durapatite
keywords: Biological property; Bone tissue engineering; Functionally graded materials; Mechanical property; Two-step sintering