title: Effect of heavy metals on acdS gene expression in Herbaspirillium sp. GW103 isolated from rhizosphere soil.
abstract: This study aimed to understand the influence of heavy metals on 1-aminocyclopropane-1-carboxylate deaminase activity (ACCD) and acdS gene expression in Herbaspirillium sp. GW103. The GW103 strain ACCD activity decreased in cells grown in a medium supplemented with Pb and As, whereas cells grown in medium supplemented with Cu showed increase in enzyme activity. The GW103 strain produced 262.2 ± 6.17 μmol of α-ketobutyrate per milligram of protein per hour during ACC deamination at 25 °C after 24 h incubation. Using a PCR approach, an acdS coding-gene of 1.06 kbp was amplified in isolate GW103, showing 92% identity with Herbaspirillum seropedicae SmR1 acdS gene. Real time quantitative polymerase chain reaction results indicate that the acdS expression rate was increased (7.1-fold) in the presence of Cu, whereas it decreased (0.2- and 0.1-fold) in the presence of As and Pb.
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, South Korea.
authors.forename: Praburaman
authors.initials: P
authors.lastname: Loganathan
authors.affiliation: Department of Ecology Landscape Architecture-Design, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, South Korea.
authors.forename: Hyun
authors.initials: H
authors.lastname: Myung
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, South Korea.
authors.forename: Govarthanan
authors.initials: G
authors.lastname: Muthusamy
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, South Korea.|Plant Medical Research Center, College of Agricultural and Life Sciences, Chonbuk National University, Jeonju, Jeonbuk, South Korea.
authors.forename: Kui-Jae
authors.initials: KJ
authors.lastname: Lee
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, South Korea.
authors.forename: Kamala-Kannan
authors.initials: KK
authors.lastname: Seralathan
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, South Korea.|Plant Medical Research Center, College of Agricultural and Life Sciences, Chonbuk National University, Jeonju, Jeonbuk, South Korea.
authors.forename: Byung-Taek
authors.initials: BT
authors.lastname: Oh
mesh_terms: D001151:Arsenic; D019755:Carbon-Carbon Lyases / Q000235:genetics; D003300:Copper; D004269:DNA, Bacterial / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D042803:Herbaspirillum / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D007854:Lead; D019216:Metals, Heavy / Q000378:metabolism*; D018517:Plant Roots; D060888:Real-Time Polymerase Chain Reaction; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D019216:Metals, Heavy; D007854:Lead; D003300:Copper; C033634:1-aminocyclopropane-1-carboxylate deaminase; D019755:Carbon-Carbon Lyases; D001151:Arsenic
keywords: ACCD; Heavy metals; Herbaspirillium sp; RT-qPCR