title: Meta-regulation of Arabidopsis auxin responses depends on tRNA maturation.
abstract: Polar transport of the phytohormone auxin throughout plants shapes morphogenesis and is subject to stringent and specific control. Here, we identify basic cellular activities connected to translational control of gene expression as sufficient to specify auxin-mediated development. Mutants in subunits of Arabidopsis Elongator, a protein complex modulating translational efficiency via maturation of tRNAs, exhibit defects in auxin-controlled developmental processes, associated with reduced abundance of PIN-formed (PIN) auxin transport proteins. Similar anomalies are observed upon interference with tRNA splicing by downregulation of RNA ligase (AtRNL), pointing to a general role of tRNA maturation in auxin signaling. Elongator Protein 6 (ELP6) and AtRNL expression patterns underline an involvement in adjusting PIN protein levels, whereas rescue of mutant defects by auxin indicates rate-limiting activities in auxin-controlled organogenesis. This emphasizes mechanisms in which auxin serves as a bottleneck for plant morphogenesis, translating common cellular activities into defined developmental readouts.
authors.affiliation: Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Muthgasse 18, 1190 Wien, Austria.
authors.forename: Johannes
authors.initials: J
authors.lastname: Leitner
authors.affiliation: Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Muthgasse 18, 1190 Wien, Austria.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Retzer
authors.affiliation: Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Muthgasse 18, 1190 Wien, Austria.
authors.forename: Nenad
authors.initials: N
authors.lastname: Malenica
authors.affiliation: Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Muthgasse 18, 1190 Wien, Austria.
authors.forename: Rasa
authors.initials: R
authors.lastname: Bartkeviciute
authors.affiliation: Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Muthgasse 18, 1190 Wien, Austria.
authors.forename: Doris
authors.initials: D
authors.lastname: Lucyshyn
authors.affiliation: Department of Molecular Biology, Umeå University, 901 87 Umeå, Sweden.
authors.forename: Gunilla
authors.initials: G
authors.lastname: Jäger
authors.affiliation: Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Muthgasse 18, 1190 Wien, Austria.
authors.forename: Barbara
authors.initials: B
authors.lastname: Korbei
authors.affiliation: Department of Molecular Biology, Umeå University, 901 87 Umeå, Sweden.
authors.forename: Anders
authors.initials: A
authors.lastname: Byström
authors.affiliation: Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Muthgasse 18, 1190 Wien, Austria. Electronic address: christian.luschnig@boku.ac.at.
authors.forename: Christian
authors.initials: C
authors.lastname: Luschnig
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D007210:Indoleacetic Acids / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012343:RNA, Transfer / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; D026901:Membrane Transport Proteins; D012343:RNA, Transfer; D016601:RNA-Binding Proteins; C000605362:ELP6 protein, Arabidopsis; C116363:PIN1 protein, Arabidopsis; C117013:AT5G57090 protein, Arabidopsis