title: Comparing multi-module connections in membrane chromatography scale-up.
abstract: Membrane chromatography is increasingly used for protein purification in the biopharmaceutical industry. Membrane adsorbers are often pre-assembled by manufacturers as ready-to-use modules. In large-scale protein manufacturing settings, the use of multiple membrane modules for a single batch is often required due to the large quantity of feed material. The question as to how multiple modules can be connected to achieve optimum separation and productivity has been previously approached using model proteins and mass transport theories. In this study, we compare the performance of multiple membrane modules in series and in parallel in the production of a protein antigen. Series connection was shown to provide superior separation compared to parallel connection in the context of competitive adsorption.
authors.affiliation: Bioprocess Research and Development, Sanofi Pasteur, Toronto, Canada M2R 3T4. Electronic address: zhou.yu@sanofipasteur.com.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Bioprocess Research and Development, Sanofi Pasteur, Toronto, Canada M2R 3T4.
authors.forename: Tishtar
authors.initials: T
authors.lastname: Karkaria
authors.affiliation: Bioprocess Research and Development, Sanofi Pasteur, Toronto, Canada M2R 3T4.
authors.forename: Marianela
authors.initials: M
authors.lastname: Espina
authors.affiliation: Bioprocess Research and Development, Sanofi Pasteur, Toronto, Canada M2R 3T4.
authors.forename: Manjeet
authors.initials: M
authors.lastname: Hunjun
authors.affiliation: Bioprocess Research and Development, Sanofi Pasteur, Toronto, Canada M2R 3T4.
authors.forename: Abera
authors.initials: A
authors.lastname: Surendran
authors.affiliation: Bioprocess Research and Development, Sanofi Pasteur, Toronto, Canada M2R 3T4.
authors.forename: Tina
authors.initials: T
authors.lastname: Luu
authors.affiliation: Bioprocess Research and Development, Sanofi Pasteur, Toronto, Canada M2R 3T4.
authors.forename: Julia
authors.initials: J
authors.lastname: Telychko
authors.affiliation: Bioprocess Research and Development, Sanofi Pasteur, Toronto, Canada M2R 3T4.
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Yang
mesh_terms: D001709:Biotechnology; D002852:Chromatography, Ion Exchange / Q000295:instrumentation* / Q000379:methods*; D004867:Equipment Design; D008567:Membranes, Artificial; D011994:Recombinant Proteins / Q000302:isolation & purification
chemical_list: D008567:Membranes, Artificial; D011994:Recombinant Proteins
keywords: Bioprocess; Chromatography; Membrane adsorber; Purification; Scale-up