title: Monoclonal antibody against M protein of spring viremia of carp virus.
abstract: M protein of spring viremia of carp virus (SVCV) was expressed in Escherichia coli and then used to immunize BALB/c mice. One monoclonal antibody (5A1) against M protein was generated by fusion of mouse myeloma cell line SP2/0 and spleen lymphocytes from immunized mice. The characterizations of this MAb were confirmed by ELISA (enzyme linked immunosorbent assay), IFA (immunofluorescent assay), and Western blot analysis. All results indicate that MAb 5A1 was specific to SVCV M protein.
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University , Wuhan, Hubei, China .
authors.forename: Zeming
authors.initials: Z
authors.lastname: Li
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.forename: Peixiao
authors.initials: P
authors.lastname: Luo
authors.forename: Guangxin
authors.initials: G
authors.lastname: Liu
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Xueqin
authors.initials: X
authors.lastname: Liu
mesh_terms: D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000096:biosynthesis / Q000737:chemistry*; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000737:chemistry*; D000918:Antibody Specificity; D002347:Carps / Q000821:virology; D018604:Epitope Mapping; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D012209:Rhabdoviridae / Q000276:immunology*; D014763:Viral Matrix Proteins / Q000276:immunology* / Q000378:metabolism
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D000914:Antibodies, Viral; D014763:Viral Matrix Proteins