title: Effect of replication on epigenetic memory and consequences on gene transcription.
abstract: Gene activity in eukaryotes is in part regulated at the level of chromatin through the assembly of local chromatin states that are more or less permissive to transcription. How do these chromatin states achieve their functions and whether or not they contribute to the epigenetic inheritance of the transcriptional program remain to be elucidated. In cycling cells, stability is indeed strongly challenged by the periodic occurrence of replication and cell division. To address this question, we perform simulations of the stochastic dynamics of chromatin states when driven out-of-equilibrium by periodic perturbations. We show how epigenetic memory is significantly affected by the cell cycle length. In addition, we develop a simple model to connect the epigenetic state to the transcriptional state and gene activity. In particular, it suggests that replication may induce transcriptional bursting at repressive loci. Finally, we discuss how our findings-effect of replication and link to gene transcription-have original and deep implications to various biological contexts of epigenetic memory.
authors.affiliation: École Normale Supérieure de Lyon, CNRS, Laboratoire de Physique, UMR 5672, Lyon, France.
authors.forename: Mehari B
authors.initials: MB
authors.lastname: Zerihun
authors.forename: Cédric
authors.initials: C
authors.lastname: Vaillant
authors.forename: Daniel
authors.initials: D
authors.lastname: Jost
mesh_terms: D002453:Cell Cycle; D002455:Cell Division; D004261:DNA Replication; D044127:Epigenesis, Genetic; D056890:Eukaryota / Q000235:genetics; D008957:Models, Genetic; D009010:Monte Carlo Method; D013269:Stochastic Processes; D014158:Transcription, Genetic