title: Simultaneous determination of doxorubicin and curcumin in rat plasma by LC-MS/MS and its application to pharmacokinetic study.
abstract: A specific, sensitive and rapid liquid chromatography tandem mass spectrometry (LC-MS/MS) method has been developed and validated for simultaneous quantification of doxorubicin and curcumin in rat plasma after intravenous administration. The analytes of doxorubicin and curcumin were extracted with methanol precipitation using glibenclamide as internal standard (IS). The chromatographic separation was performed on a C18 column with acetonitrile and 0.1% formic acid water as mobile phase and with gradient elution at a flow rate of 0.2 mL/min. Calibration curves were linear over the ranges of 2-8000 ng/mL for doxorubicin and 5-2000 ng/mL for curcumin (r > 0.99). The lower limit of quantification (LLOQ) was 2 ng/mL for DOX and 5 ng/mL for Cur. Finally, this developed method was successfully applied in the pharmacokinetic study of doxorubicin and curcumin in rats and evaluated the effects of curcumin on the absorption of doxorubicin after intravenous administration.
authors.affiliation: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, People's Republic of China; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, People's Republic of China.
authors.forename: Wenzhuan
authors.initials: W
authors.lastname: Ma
authors.affiliation: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, People's Republic of China.
authors.forename: Jinling
authors.initials: J
authors.lastname: Wang
authors.affiliation: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, People's Republic of China; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, People's Republic of China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, People's Republic of China. Electronic address: pengfeitu@163.com.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Tu
mesh_terms: D000818:Animals; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods; D003474:Curcumin / Q000737:chemistry* / Q000493:pharmacokinetics*; D004317:Doxorubicin / Q000737:chemistry* / Q000493:pharmacokinetics*; D008297:Male; D010949:Plasma / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D004317:Doxorubicin; D003474:Curcumin
keywords: Curcumin; Doxorubicin; LC–MS/MS; Methanol precipitation; Pharmacokinetic study