title: Myosin heavy chain 10 (MYH10) is required for centriole migration during the biogenesis of primary cilia.
abstract: The actin cytoskeleton has been implicated in the assembly of cilia, but roles of actin-dependent motor proteins in ciliogenesis remain unclear. Myosin heavy chain 10 (MYH10), one of the isoforms of non-muscle myosin II, is known to mediate centrosome reorientation during cell migration. Here we show that MYH10 is required for centriole migration to the apical plasma membrane, which occurs at the onset of ciliogenesis. Knockdown of MYH10 in RPE1 cells caused a reduction in the levels of cortical filamentous actin (F-actin) and its binding protein EZRIN. Moreover, both centriole migration and subsequent cilium assembly were defective in MYH10 depleted cells. We further found that MYH10 influences centrosomal recruitment of IFT88, which is required for the transport of building blocks to the ciliary tip. The role of MYH10 in IFT88 recruitment appears to be indirect in that there is a correlation between centriolar IFT88 levels and centriolar positions along the apical-basal axis during ciliogenesis. Our results indicate that MYH10 contributes to ciliogenesis in RPE1 cells by promoting cortical actin-dependent centriole migration.
authors.affiliation: Graduate School of Medical Science and Engineering, KAIST, Daejeon 305-701, Republic of Korea.
authors.forename: Hyowon
authors.initials: H
authors.lastname: Hong
authors.affiliation: Graduate School of Medical Science and Engineering, KAIST, Daejeon 305-701, Republic of Korea.
authors.forename: Jongshin
authors.initials: J
authors.lastname: Kim
authors.affiliation: Graduate School of Medical Science and Engineering, KAIST, Daejeon 305-701, Republic of Korea. Electronic address: joonkim@kaist.ac.kr.
authors.forename: Joon
authors.initials: J
authors.lastname: Kim
mesh_terms: D000199:Actins / Q000378:metabolism*; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology*; D002502:Centrioles / Q000502:physiology*; D002923:Cilia / Q000502:physiology* / Q000648:ultrastructure*; D006801:Humans; D009024:Morphogenesis / Q000502:physiology*; D018995:Myosin Heavy Chains / Q000378:metabolism*; D024501:Nonmuscle Myosin Type IIB / Q000378:metabolism*; D055213:Retinal Pigment Epithelium / Q000166:cytology / Q000502:physiology
chemical_list: D000199:Actins; D024501:Nonmuscle Myosin Type IIB; C103815:nonmuscle myosin type IIB heavy chain; D018995:Myosin Heavy Chains
keywords: Actin; Centriole migration; IFT88; MYH10; Primary cilia