title: Inhibition of As(III) and Hg(II) caused aortic hypercontraction by eugenol, linalool and carvone.
abstract: Acute and chronic exposure to arsenic and mercury is known to produce vasoconstriction. There is, however, no clarity concerning the pathways leading to this increased contraction. In this study we elicit and compare maximum contractility of rat aortas under resting conditions in the presence of arsenic and mercury, and delineate pathways mediating this effect. Phenylephrine (PE) induced hypercontraction of 37% and 32% were obtained when isolated aortic segments were exposed to 25 ?M As(III) and 6 nM Hg(II), respectively. Isometric contraction measurements in presence of apocynin, verapamil and sodium nitroprusside indicates that the major causes of increased contraction are reactive oxygen species (ROS) and depletion of nitric oxide (NO). Calcium influx plays a minor role in arsenic and mercury caused hypercontraction. In unexposed aorta, eugenol causes relaxation by inhibiting ROS and elevating NO, linalool by blocking voltage dependent calcium channel (VDCC) and elevating NO, and carvone by blocking calcium influx through VDDC. Since the arsenic and mercury hypercontraction is mediated by increased ROS and depleted NO, we hypothesize that molecules which neutralize ROS or elevate NO will be better ameliorators. In line with this argument, we found eugenol to be the best ameliorator of arsenic and mercury hypercontraction followed by linalool and carvone.
authors.affiliation: Department of Biosciences, Faculty of Natural Sciences, Jamia Millia Islamia, New Delhi, India.
authors.forename: Swati
authors.initials: S
authors.lastname: Kundu
authors.forename: Hiba
authors.initials: H
authors.lastname: Shabir
authors.forename: Seemi Farhat
authors.initials: SF
authors.lastname: Basir
authors.forename: Luqman Ahmad
authors.initials: LA
authors.lastname: Khan
mesh_terms: D000080462:Acyclic Monoterpenes; D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects*; D001152:Arsenicals / Q000037:antagonists & inhibitors*; D002118:Calcium / Q000378:metabolism; D000081005:Cyclohexane Monoterpenes; D005054:Eugenol / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D017669:Mercury Compounds / Q000037:antagonists & inhibitors*; D039821:Monoterpenes / Q000494:pharmacology*; D009569:Nitric Oxide / Q000378:metabolism; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D014661:Vasoconstriction / Q000187:drug effects*
chemical_list: D000080462:Acyclic Monoterpenes; D001152:Arsenicals; D000081005:Cyclohexane Monoterpenes; D017669:Mercury Compounds; D039821:Monoterpenes; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D005054:Eugenol; C006923:carvone; C018584:linalool; D002118:Calcium