title: Gamma-tocotrienol treatment increased peroxiredoxin-4 expression in HepG2 liver cancer cell line.
abstract: BACKGROUND
  To determine the antiproliferative effect of gamma-tocotrienol (GTT) treatment on differential protein expression in HepG2 cells.
  
  
  METHODS
  HepG2 cells were treated with 70 μM GTT for 48 hours and differentially expressed protein spots were determined by two-dimensional electrophoresis (2DE), identified by MALDI-TOF mass spectrometer (MS) and validated by quantitative real-time polymerase chain reaction (qRT-PCR).
  
  
  RESULTS
  GTT treatment on HepG2 cells showed a total of five differentially expressed proteins when compared to their respective untreated cells where three proteins were down-regulated and two proteins were up-regulated. One of these upregulated proteins was identified as peroxiredoxin-4 (Prx4). Validation by qRT-PCR however showed decreased expression of Prx4 mRNA in HepG2 cells following GTT treatment.
  
  
  CONCLUSIONS
  GTT might directly influence the expression dynamics of peroxiredoxin-4 to control proliferation in liver cancer.
authors.affiliation: Department of Biochemistry, Universiti Kebangsaan Malaysia Medical Centre, Kuala Lumpur, Malaysia. farahsazli@gmail.com.
authors.forename: Farahani
authors.initials: F
authors.lastname: Abdul Rahman Sazli
authors.affiliation: Department of Biochemistry, Universiti Kebangsaan Malaysia Medical Centre, Kuala Lumpur, Malaysia. zakiah@medic.ukm.my.
authors.forename: Zakiah
authors.initials: Z
authors.lastname: Jubri
authors.affiliation: Department of Clinical Oral Biology, Faculty of Dentistry, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia. mariati_ar@dental.ukm.my.
authors.forename: Mariati
authors.initials: M
authors.lastname: Abdul Rahman
authors.affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya and University of Malaya Centre for Proteomics Research (UMCPR), Kuala Lumpur, Malaysia. saiful72@um.edu.my.
authors.forename: Saiful Anuar
authors.initials: SA
authors.lastname: Karsani
authors.affiliation: Malaysian Palm Oil Board, Bangi, Selangor, Malaysia. gapor@mpob.gov.my.
authors.forename: Abdul Gapor
authors.initials: AG
authors.lastname: Md Top
authors.affiliation: Department of Biochemistry, Universiti Kebangsaan Malaysia Medical Centre, Kuala Lumpur, Malaysia. zurina@medic.ukm.my.
authors.forename: Wan Zurinah
authors.initials: WZ
authors.lastname: Wan Ngah
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D001650:Bile Duct Neoplasms; D002839:Chromans / Q000378:metabolism; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D056945:Hep G2 Cells; D018197:Hepatoblastoma / Q000188:drug therapy / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008113:Liver Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D054464:Peroxiredoxins / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D040901:Proteomics; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D024508:Tocotrienols / Q000494:pharmacology* / Q000627:therapeutic use; D015854:Up-Regulation; D014810:Vitamin E / Q000031:analogs & derivatives / Q000378:metabolism; D014815:Vitamins / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D002839:Chromans; D011506:Proteins; D012333:RNA, Messenger; D024508:Tocotrienols; D014815:Vitamins; D014810:Vitamin E; C013649:plastochromanol 8; D054464:Peroxiredoxins