title: The Protective Effects of Sufentanil Pretreatment on Rat Brains under the State of Cardiopulmonary Bypass.
abstract: This study aimed to observe the protective effects of sufentanil pretreatment on rat cerebral injury during cardiopulmonary bypass (CPB) and to explore the underlying mechanism. Twenty-four male adult Sprague Dawley (SD) rats were divided into 4 groups. Then, the rat CPB model was established. A 14G trocar was inserted into the atrium dextrum. For rats in S1 and S5 groups, sufentanil (1 µgKg(-1) and 5 µgKg(-1)) were applied before CPB process. After the operation, rat brain samples were harvested for measurement of the water content of the brains, total calcium in brain tissue and the level of serum S100β. Compared with the Sham group, the water content and the total calcium of the brain tissue, and the expression of S100β in serum were significantly increased in the CPB group (P<0.05). Compared with the CPB group, sufentanil treatment significantly reduced the water content of the brains, the total calcium and S100β expression (P<0.05). The blood pressure and heart rate were significantly decreased in groups CPB, S1, and S5 compared with Sham group during CPB. Compared with the Sham group, the levels of pH and blood lactate in other groups were decreased and increased, respectively, in the post-CPB period. During the CPB and post-CPB periods, the hematocrit levels were significantly down-regulated in groups CPB, S1, and S5 compared with Sham group. In conclusion, sufentanil pretreatment was effective in reducing the cerebral injury during CPB. Reduction in calcium overload may be a potential mechanism in such process.
authors.affiliation: Department of Anesthesiology, Jingzhou Clinical Medical College, Yangtze University, Jingzhou, Hubei Province,. China .
authors.forename: Kun
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Department of Oncology, Jingzhou Clinical Medical College, Yangtze University, Jingzhou, Hubei Province, China.
authors.forename: Man
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Pathophysiology, Medical School of Yangtze University, Jingzhou, Hubei Province, China .
authors.forename: Xiao-Chun
authors.initials: XC
authors.lastname: Peng
authors.affiliation: Department of Anesthesiology, Jingzhou Clinical Medical College, Yangtze University, Jingzhou, Hubei Province,. China .
authors.forename: Li-Shen
authors.initials: LS
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, Jingzhou Clinical Medical College, Yangtze University, Jingzhou, Hubei Province,. China .
authors.forename: Ai-Ping
authors.initials: AP
authors.lastname: Dong
authors.affiliation: Department of Anesthesiology, Jingzhou Clinical Medical College, Yangtze University, Jingzhou, Hubei Province,. China .
authors.forename: Shu-Wei
authors.initials: SW
authors.lastname: Shen
authors.affiliation: Department of Anesthesiology, Jingzhou Clinical Medical College, Yangtze University, Jingzhou, Hubei Province,. China .
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
keywords: CPB; Pretreatment; S100β; Sufentanil; Total calcium in brain tissue