title: A novel antibody-antigen based impedimetric immunosensor for low level detection of HER2 in serum samples of breast cancer patients via modification of a gold nanoparticles decorated multiwall carbon nanotube-ionic liquid electrode.
abstract: A highly sensitive impedimetric immunosensor based on a gold nanoparticles/multiwall carbon nanotube-ionic liquid electrode (AuNPs/MW-CILE) was developed for the determination of human epidermal growth factor receptor 2 (HER2). Gold nanoparticles were used to enhance the extent of immobilization and to retain the immunoactivity of the antibody Herceptin on the electrode. Cyclic voltammetry and electrochemical impedance spectroscopy were employed for characterization of various layers coated onto the AuNPs/MW-CILE. The impedance measurements at different steps were based on the charge transfer kinetics of the [Fe(CN)6](3-/4-) redox pair. The immobilization of antibody and the corresponding antigen-antibody interaction at the electrode surface altered the interfacial electron transfer. The interactions of antibody with various concentrations of antigen were also monitored via the change of impedance response. The results showed that the charge transfer resistance increases linearly with increasing concentrations of HER2 antigen. The linear range and limit of detection were found as 10-110 ng mL(-1) and 7.4 ng mL(-1), respectively. The sensitivity and specificity of the immunosensor were validated. The results showed that the prepared immunosensor is a useful tool for screening of trace amounts of HER2 in serum samples of breast cancer patients.
authors.affiliation: Nano Drug Delivery Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors.forename: Elham
authors.initials: E
authors.lastname: Arkan
authors.affiliation: Department of Medical Nanotechnology, School of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, Iran; Research Center for Science and Technology in Medicine, Imam Khomeini Hospital, Tehran, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Saber
authors.affiliation: Department of Chemistry, Payame Noor University, P.O. Box 19395-3697, Tehran, Iran.
authors.forename: Ziba
authors.initials: Z
authors.lastname: Karimi
authors.affiliation: Department of Chemistry, Razi University, Kermanshah, Iran. Electronic address: mshamsipur@yahoo.com.
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Shamsipur
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation; D001943:Breast Neoplasms / Q000097:blood*; D003432:Cross-Linking Reagents / Q000737:chemistry; D058266:Dielectric Spectroscopy / Q000295:instrumentation*; D004566:Electrodes; D005260:Female; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D052578:Ionic Liquids / Q000737:chemistry; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D037742:Nanotubes, Carbon / Q000737:chemistry*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000097:blood*; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: D055910:Antibodies, Immobilized; D003432:Cross-Linking Reagents; D006046:Gold; D052578:Ionic Liquids; D037742:Nanotubes, Carbon; D018719:Erb-b2 Receptor Tyrosine Kinases; D013438:Sulfhydryl Compounds; C508053:ERBB2 protein, human; C501433:1,6-hexanedithiol
keywords: Carbon ionic liquid electrode; Electrochemical impedance spectroscopy; Gold nanoparticles; HER2 antibody (Herceptin); Human epidermal growth factor receptor 2 (HER2) immunosensor; Multiwall carbon nanotubes