title: The Potential Application of Mitochondrial Medicine in Toxicologic Poisoning.
abstract: The advancement of biomolecular techniques has continued to advance in the area of mitochondrial medicine. This has allowed clinicians and researchers to more effectively study the bioenergetics of the mitochondria in various disease states. One potential technique in mitochondrial medicine is the generation of cytoplasmic hybrids. A cytoplasmic hybrid or cybrid are created by introducing mitochondrial DNA (mtDNA) of interest into cells depleted of mtDNA. A cybrid is therefore a hybrid cell that mixes the nuclear genome from one cell with the mitochondrial genes from another cell. Cybrids are currently utilized in mitochondrial research to demonstrate mitochondrial involvement in a wide range of diseases that include diabetes, Parkinson's disease and inherited diseases. At this time the use of cybrids to study toxicologic poisoning is limited and offers a potential avenue of research in this area.
authors.affiliation: Center for Resuscitation Science and Center for Mitochondrial and Epigenomic Medicine, Department of Emergency Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, USA, david.jang@uphs.upenn.edu.
authors.forename: David H
authors.initials: DH
authors.lastname: Jang
authors.forename: Joshua W
authors.initials: JW
authors.lastname: Lampe
authors.forename: Lance B
authors.initials: LB
authors.lastname: Becker
mesh_terms: D000818:Animals; D016678:Genome; D006801:Humans; D006822:Hybrid Cells; D008928:Mitochondria / Q000235:genetics* / Q000378:metabolism*; D011041:Poisoning / Q000235:genetics* / Q000378:metabolism*; D014116:Toxicology / Q000379:methods*