title: Proteomic profiling of lysine acetylation in Pseudomonas aeruginosa reveals the diversity of acetylated proteins.
abstract: Protein lysine acetylation is a reversible and highly regulated post-translational modification with the well demonstrated physiological relevance in eukaryotes. Recently, its important role in the regulation of metabolic processes in bacteria was highlighted. Here, we reported the lysine acetylproteome of Pseudomonas aeruginosa using a proteomic approach. We identified 430 unique peptides corresponding to 320 acetylated proteins. In addition to the proteins involved in various metabolic pathways, several enzymes contributing to the lipopolysaccharides biosynthesis were characterized as acetylated. This data set illustrated the abundance and the diversity of acetylated lysine proteins in P. aeruginosa and opens opportunities to explore the role of the acetylation in the bacterial physiology.
authors.affiliation: CNRS, UMR 6270, Polymères, Biopolymères, Surfaces Laboratory, Mont-Saint-Aignan, France.|Normandie University, UR, France.|PISSARO proteomic facility, IRIB, Mont-Saint-Aignan, France.
authors.forename: Tassadit
authors.initials: T
authors.lastname: Ouidir
authors.affiliation: CNRS, UMR 6270, Polymères, Biopolymères, Surfaces Laboratory, Mont-Saint-Aignan, France.|Normandie University, UR, France.|PISSARO proteomic facility, IRIB, Mont-Saint-Aignan, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Cosette
authors.affiliation: CNRS, UMR 6270, Polymères, Biopolymères, Surfaces Laboratory, Mont-Saint-Aignan, France.|Normandie University, UR, France.|PISSARO proteomic facility, IRIB, Mont-Saint-Aignan, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Jouenne
authors.affiliation: CNRS, UMR 6270, Polymères, Biopolymères, Surfaces Laboratory, Mont-Saint-Aignan, France.|Normandie University, UR, France.|PISSARO proteomic facility, IRIB, Mont-Saint-Aignan, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Hardouin
mesh_terms: D000107:Acetylation; D008070:Lipopolysaccharides / Q000378:metabolism*; D008239:Lysine / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D011550:Pseudomonas aeruginosa / Q000378:metabolism*
chemical_list: D008070:Lipopolysaccharides; D008239:Lysine
keywords: Lysine acetylation; Mass spectrometry; Microbiology; Pseudomonas aeruginosa