title: Comprehensive Metabolomic, Lipidomic and Microscopic Profiling of Yarrowia lipolytica during Lipid Accumulation Identifies Targets for Increased Lipogenesis.
abstract: Yarrowia lipolytica is an oleaginous ascomycete yeast that accumulates large amounts of lipids and has potential as a biofuel producing organism. Despite a growing scientific literature focused on lipid production by Y. lipolytica, there remain significant knowledge gaps regarding the key biological processes involved. We applied a combination of metabolomic and lipidomic profiling approaches as well as microscopic techniques to identify and characterize the key pathways involved in de novo lipid accumulation from glucose in batch cultured, wild-type Y. lipolytica. We found that lipids accumulated rapidly and peaked at 48 hours during the five day experiment, concurrent with a shift in amino acid metabolism. We also report that exhaustion of extracellular sugars coincided with thickening of the cell wall, suggesting that genes involved in cell wall biogenesis may be a useful target for improving the efficiency of lipid producing yeast strains.
authors.affiliation: Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, United States of America.
authors.forename: Kyle R
authors.initials: KR
authors.lastname: Pomraning
authors.affiliation: Fundamental and Computer Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA, United States of America.
authors.forename: Siwei
authors.initials: S
authors.lastname: Wei
authors.affiliation: Energy and Environment Directorate, Pacific Northwest National Laboratory, Richland, WA, United States of America.
authors.forename: Sue A
authors.initials: SA
authors.lastname: Karagiosis
authors.affiliation: Fundamental and Computer Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA, United States of America.
authors.forename: Young-Mo
authors.initials: YM
authors.lastname: Kim
authors.affiliation: Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, United States of America.
authors.forename: Alice C
authors.initials: AC
authors.lastname: Dohnalkova
authors.affiliation: Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, United States of America.
authors.forename: Bruce W
authors.initials: BW
authors.lastname: Arey
authors.affiliation: Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, United States of America.
authors.forename: Erin L
authors.initials: EL
authors.lastname: Bredeweg
authors.affiliation: Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, United States of America.
authors.forename: Galya
authors.initials: G
authors.lastname: Orr
authors.affiliation: Fundamental and Computer Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA, United States of America.
authors.forename: Thomas O
authors.initials: TO
authors.lastname: Metz
authors.affiliation: Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, United States of America.
authors.forename: Scott E
authors.initials: SE
authors.lastname: Baker
mesh_terms: D002849:Chromatography, Gas; D002853:Chromatography, Liquid; D050356:Lipid Metabolism; D050155:Lipogenesis; D013058:Mass Spectrometry; D055432:Metabolomics; D025062:Yarrowia / Q000378:metabolism*