title: An electrochemiluminescence immunosensor for thyroid stimulating hormone based on polyamidoamine-norfloxacin functionalized Pd-Au core-shell hexoctahedrons as signal enhancers.
abstract: In this work, a novel polyamidoamine-norfloxacin (PAMAM-NFLX) complex and core-shell Pd-Au hexoctahedrons (Pd@Au HOHs) as enhancers are employed for development of a sensitive sandwich-type electrochemiluminescence (ECL) immunosensor to detect thyroid stimulating hormone (TSH). Here, norfloxacin (NFLX) is decorated abundantly on the surface of polyamidoamine (PAMAM) dendrimer via amide linkage to form PAMAM-NFLX complex. Thus, the resultant PAMAM-NFLX can serve as a novel co-reactant to efficiently amplify the ECL signal of peroxydisulfate-oxygen (S2O8(2-)-O2) system. Pd@Au HOHs were used as nano-carriers to assemble detection antibody (Ab2) and the PAMAM-NFLX complex. Besides, it can further enhance the ECL signal by promoting the generation of intermediate free radical HO(•) during the ECL reaction of S2O8(2-)-O2 system. The proposed immunosensor shows high sensitivity and specificity, and responds linearly to the concentration of TSH from 0.05 to 20 μIU mL(-1) with a low detection limit of 0.02 μIU mL(-1) (S/N=3). Moreover, the immunosensor successfully achieves the detection of TSH in practical human blood serum with desirable results.
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Qiqi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Haijun
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China. Electronic address: yqchai@swu.edu.cn.
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Chai
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China. Electronic address: yuanruo@swu.edu.cn.
authors.forename: Ruo
authors.initials: R
authors.lastname: Yuan
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation; D050091:Dendrimers / Q000737:chemistry; D055664:Electrochemical Techniques / Q000295:instrumentation*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000295:instrumentation*; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D009643:Norfloxacin / Q000737:chemistry*; D010165:Palladium / Q000737:chemistry*; D011073:Polyamines / Q000737:chemistry*; D013972:Thyrotropin / Q000097:blood*
chemical_list: D050091:Dendrimers; C531249:Poly(amidoamine); D011073:Polyamines; D010165:Palladium; D006046:Gold; D013972:Thyrotropin; D009643:Norfloxacin
keywords: Co-reactant; Electrochemiluminescence; Hexoctahedrons; Peroxydisulfate-oxygen system; Thyroid stimulating hormone