title: Faecalibacterium prausnitzii Strain HTF-F and Its Extracellular Polymeric Matrix Attenuate Clinical Parameters in DSS-Induced Colitis.
abstract: A decrease in the abundance and biodiversity of intestinal bacteria within the Firmicutes phylum has been associated with inflammatory bowel disease (IBD). In particular, the anti-inflammatory bacterium Faecalibacterium prausnitzii, member of the Firmicutes phylum and one of the most abundant species in healthy human colon, is underrepresented in the microbiota of IBD patients. The aim of this study was to investigate the immunomodulatory properties of F. prausnitzii strain A2-165, the biofilm forming strain HTF-F and the extracellular polymeric matrix (EPM) isolated from strain HTF-F. For this purpose, the immunomodulatory properties of the F. prausnitzii strains and the EPM were studied in vitro using human monocyte-derived dendritic cells. Then, the capacity of the F. prausnitzii strains and the EPM of HTF-F to suppress inflammation was assessed in vivo in the mouse dextran sodium sulphate (DSS) colitis model. The F. prausnitzii strains and the EPM had anti-inflammatory effects on the clinical parameters measured in the DSS model but with different efficacy. The immunomodulatory effects of the EPM were mediated through the TLR2-dependent modulation of IL-12 and IL-10 cytokine production in antigen presenting cells, suggesting that it contributes to the anti-inflammatory potency of F. prausnitzii HTF-F. The results show that F. prausnitzii HTF-F and its EPM may have a therapeutic use in IBD.
authors.affiliation: Host-Microbe Interactomics Group, Animal Science Department, University of Wageningen, Wageningen, The Netherlands.
authors.forename: Oriana
authors.initials: O
authors.lastname: Rossi
authors.affiliation: Department of Cell Biology, section Membrane Cell Biology, University Medical Center Groningen, Groningen, The Netherlands.
authors.forename: M Tanweer
authors.initials: MT
authors.lastname: Khan
authors.affiliation: Institute of Microbiology of the Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Schwarzer
authors.affiliation: Institute of Microbiology of the Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Tomas
authors.initials: T
authors.lastname: Hudcovic
authors.affiliation: Institute of Microbiology of the Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Dagmar
authors.initials: D
authors.lastname: Srutkova
authors.affiliation: Microbial Ecology Group, Rowett Institute of Nutrition and Health, University of Aberdeen, Aberdeen, United Kingdom.
authors.forename: Sylvia H
authors.initials: SH
authors.lastname: Duncan
authors.affiliation: Host-Microbe Interactomics Group, Animal Science Department, University of Wageningen, Wageningen, The Netherlands.
authors.forename: Ellen H
authors.initials: EH
authors.lastname: Stolte
authors.affiliation: Institute of Microbiology of the Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Hana
authors.initials: H
authors.lastname: Kozakova
authors.affiliation: Microbial Ecology Group, Rowett Institute of Nutrition and Health, University of Aberdeen, Aberdeen, United Kingdom.
authors.forename: Harry J
authors.initials: HJ
authors.lastname: Flint
authors.affiliation: Department of Pediatrics, Division of Gastroenterology and Nutrition, Erasmus Medical Center-Sophia Children's Hospital, Rotterdam, The Netherlands.
authors.forename: Janneke N
authors.initials: JN
authors.lastname: Samsom
authors.affiliation: Department of Cell Biology, section Membrane Cell Biology, University Medical Center Groningen, Groningen, The Netherlands.
authors.forename: Hermie J M
authors.initials: HJ
authors.lastname: Harmsen
authors.affiliation: Host-Microbe Interactomics Group, Animal Science Department, University of Wageningen, Wageningen, The Netherlands.
authors.forename: Jerry M
authors.initials: JM
authors.lastname: Wells
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism; D003092:Colitis / Q000139:chemically induced / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism; D016264:Dextran Sulfate / Q000009:adverse effects; D004195:Disease Models, Animal; D005109:Extracellular Matrix / Q000378:metabolism*; D005260:Female; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism; D018836:Inflammation Mediators / Q000378:metabolism; D015212:Inflammatory Bowel Diseases / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D007413:Intestinal Mucosa / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism; D051379:Mice; D010641:Phenotype; D045857:Ruminococcus / Q000378:metabolism* / Q000648:ultrastructure; D013154:Spleen / Q000276:immunology / Q000378:metabolism; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D000954:Antigens, Surface; D016207:Cytokines; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D018836:Inflammation Mediators; D051195:Toll-Like Receptor 2; D016264:Dextran Sulfate