title: Consequences of lower food intake on the digestive enzymes activities, the energy reserves and the reproductive outcome in Gammarus fossarum.
abstract: Digestive enzyme activity is often used as a sensitive response to environmental pollution. However, only little is known about the negative effects of stress on digestive capacities and their consequences on energy reserves and reproduction, although these parameters are important for the maintenance of populations. To highlight if changes in biochemical responses (digestive enzymes and reserves) led to impairments at an individual level (fertility), Gammarus fossarum were submitted to a lower food intake throughout a complete female reproductive cycle (i.e. from ovogenesis to offspring production). For both males and females, amylase activity was inhibited by the diet stress, whereas trypsin activity was not influenced. These results underline similar sensitivity of males and females concerning their digestive capacity. Energy reserves decreased with food starvation in females, and remained stable in males. The number of embryos per female decreased with food starvation. Lower digestive activity in males and females therefore appears as an early response. These results underline the ecological relevance of digestive markers, as they make it possible to anticipate upcoming consequences on reproduction in females, a key biological variable for population dynamics.
authors.affiliation: Université Reims Champagne Ardenne, Unité Mixte de Recherche-Ineris (UMR-I02) Stress Environnementaux et Biosurveillance des milieux aquatiques, Unité de Formation et de Recherche Sciences Exactes et Naturelles, Moulin de la Housse, Reims Cedex 2, France.
authors.forename: Laetitia
authors.initials: L
authors.lastname: Charron
authors.affiliation: Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture, Unité de Recherche Milieux Aquatiques, Ecologie et Pollutions, Ecotoxicologie, Villeurbanne Cedex, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Geffard
authors.affiliation: Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture, Unité de Recherche Milieux Aquatiques, Ecologie et Pollutions, Ecotoxicologie, Villeurbanne Cedex, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Chaumot
authors.affiliation: Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture, Unité de Recherche Milieux Aquatiques, Ecologie et Pollutions, Ecotoxicologie, Villeurbanne Cedex, France.
authors.forename: Romain
authors.initials: R
authors.lastname: Coulaud
authors.affiliation: Université Reims Champagne Ardenne, Unité Mixte de Recherche-Ineris (UMR-I02) Stress Environnementaux et Biosurveillance des milieux aquatiques, Unité de Formation et de Recherche Sciences Exactes et Naturelles, Moulin de la Housse, Reims Cedex 2, France.
authors.forename: Ali
authors.initials: A
authors.lastname: Jaffal
authors.affiliation: Université Reims Champagne Ardenne, Unité Mixte de Recherche-Ineris (UMR-I02) Stress Environnementaux et Biosurveillance des milieux aquatiques, Unité de Formation et de Recherche Sciences Exactes et Naturelles, Moulin de la Housse, Reims Cedex 2, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Gaillet
authors.affiliation: Université Reims Champagne Ardenne, Unité Mixte de Recherche-Ineris (UMR-I02) Stress Environnementaux et Biosurveillance des milieux aquatiques, Unité de Formation et de Recherche Sciences Exactes et Naturelles, Moulin de la Housse, Reims Cedex 2, France.
authors.forename: Odile
authors.initials: O
authors.lastname: Dedourge-Geffard
authors.affiliation: Université Reims Champagne Ardenne, Unité Mixte de Recherche-Ineris (UMR-I02) Stress Environnementaux et Biosurveillance des milieux aquatiques, Unité de Formation et de Recherche Sciences Exactes et Naturelles, Moulin de la Housse, Reims Cedex 2, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Geffard
mesh_terms: D033304:Amphipoda / Q000254:growth & development; D000681:Amylases / Q000378:metabolism*; D000818:Animals; D004064:Digestive System / Q000201:enzymology*; D004435:Eating; D004734:Energy Metabolism; D004784:Environmental Monitoring; D005247:Feeding Behavior; D005260:Female; D005298:Fertility; D008297:Male; D012098:Reproduction / Q000502:physiology*; D013217:Starvation; D014357:Trypsin / Q000378:metabolism*
chemical_list: D000681:Amylases; D014357:Trypsin