title: Noncoding RNAs that associate with YB-1 alter proliferation in prostate cancer cells.
abstract: The highly conserved, multifunctional YB-1 is a powerful breast cancer prognostic indicator. We report on a pervasive role for YB-1 in which it associates with thousands of nonpolyadenylated short RNAs (shyRNAs) that are further processed into small RNAs (smyRNAs). Many of these RNAs have previously been identified as functional noncoding RNAs (http://www.johnlab.org/YB1). We identified a novel, abundant, 3'-modified short RNA antisense to Dicer1 (Shad1) that colocalizes with YB-1 to P-bodies and stress granules. The expression of Shad1 was shown to correlate with that of YB-1 and whose inhibition leads to an increase in cell proliferation. Additionally, Shad1 influences the expression of additional prognostic markers of cancer progression such as DLX2 and IGFBP2. We propose that the examination of these noncoding RNAs could lead to better understanding of prostate cancer progression.
authors.affiliation: Department of Computational and Systems Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15260, USA.
authors.forename: Teresa T
authors.initials: TT
authors.lastname: Liu
authors.affiliation: Department of Computational and Systems Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15260, USA.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Arango-Argoty
authors.affiliation: Department of Computational and Systems Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15260, USA.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Computational and Systems Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15260, USA.
authors.forename: Yuefeng
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Department of Computational and Systems Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15260, USA.
authors.forename: Sang Woo
authors.initials: SW
authors.lastname: Kim
authors.affiliation: University of Regensburg, Biochemistry I, 93053 Regensburg, Bavaria, Germany.
authors.forename: Anne
authors.initials: A
authors.lastname: Dueck
authors.affiliation: Helicos BioSciences Corporation, Cambridge, Massachusetts 02139, USA.
authors.forename: Fatih
authors.initials: F
authors.lastname: Ozsolak
authors.affiliation: Department of Neurobiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15260, USA.
authors.forename: A Paula
authors.initials: AP
authors.lastname: Monaghan
authors.affiliation: University of Regensburg, Biochemistry I, 93053 Regensburg, Bavaria, Germany.
authors.forename: Gunter
authors.initials: G
authors.lastname: Meister
authors.affiliation: Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15260, USA.
authors.forename: Donald B
authors.initials: DB
authors.lastname: DeFranco
authors.affiliation: Department of Computational and Systems Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15260, USA.
authors.forename: Bino
authors.initials: B
authors.lastname: John
mesh_terms: D000818:Animals; D019556:COS Cells; D066146:Cell Body / Q000378:metabolism*; D049109:Cell Proliferation; D002522:Chlorocebus aethiops; D053487:DEAD-box RNA Helicases / Q000037:antagonists & inhibitors; D015972:Gene Expression Regulation, Neoplastic; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D061986:MCF-7 Cells; D008297:Male; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D022661:RNA, Untranslated / Q000235:genetics / Q000378:metabolism*; D043244:Ribonuclease III / Q000037:antagonists & inhibitors; D017423:Sequence Analysis, RNA; D051840:Y-Box-Binding Protein 1 / Q000235:genetics* / Q000378:metabolism
chemical_list: D022661:RNA, Untranslated; C000600066:Shad1 noncoding RNA, human; D051840:Y-Box-Binding Protein 1; C070856:YBX1 protein, human; C408087:DICER1 protein, human; D043244:Ribonuclease III; D053487:DEAD-box RNA Helicases
keywords: YB-1; miRNAs; noncoding RNAs; prostate cancer