title: The critical glucose concentration for respiration-independent proliferation of fission yeast, Schizosaccharomyces pombe.
abstract: Glucose is the fundamental energy source for life; thus cells need to respond appropriately to changes in available glucose concentration. We investigated the relationship between media glucose concentration and respiration-dependency of proliferation, using Schizosaccharomyces pombe. In media containing ≥ 0.2% glucose, neither antimycin A, an inhibitor of Complex III, nor gene deletions of essential electron transfer chain components, impaired cell division, while these factors completely inhibited cell division in media containing ≤ 0.1% glucose. These results indicate the existence of a threshold in glucose concentration that governs respiration-dependency of S. pombe proliferation.
authors.affiliation: G0 Cell Unit, Okinawa Institute of Science and Technology, 1919-1 Tancha, Onna, Okinawa 904-1111, Japan. Electronic address: takeda@center.konan-u.ac.jp.
authors.forename: Kojiro
authors.initials: K
authors.lastname: Takeda
authors.affiliation: G0 Cell Unit, Okinawa Institute of Science and Technology, 1919-1 Tancha, Onna, Okinawa 904-1111, Japan.
authors.forename: Caroline
authors.initials: C
authors.lastname: Starzynski
authors.affiliation: G0 Cell Unit, Okinawa Institute of Science and Technology, 1919-1 Tancha, Onna, Okinawa 904-1111, Japan.
authors.forename: Ayaka
authors.initials: A
authors.lastname: Mori
authors.affiliation: G0 Cell Unit, Okinawa Institute of Science and Technology, 1919-1 Tancha, Onna, Okinawa 904-1111, Japan.
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Yanagida
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D004734:Energy Metabolism; D005947:Glucose / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D005947:Glucose; D010100:Oxygen
keywords: Antimycin A; Cell cycle; Fission yeast; Glucose concentration threshold; Respiration