title: Gene expression profiles reveal effect of a high-fat diet on the development of white and brown adipose tissues.
abstract: Because of the recent discovery of brown adipose tissues tissue in adult humans, brown adipose tissues have garnered additional attention. Many studies have attempted to transform the precursor cells within the white adipocyte cultures to Brite (brown-in-white) cells by using genomic modification or pharmacological activation in order to determine the therapeutic effect of obesity. However, genome-scale analysis of the genetic factors governing the development of white and brown adipose tissues remains incomplete. In order to identify the key genes that regulate the development of white and brown adipose tissues in mice, a transcriptome analysis was performed on the adipose tissues. Network analysis of differentially expressed genes indicated that Trim30 and Ucp3 play pivotal roles in energy balance and glucose homeostasis. In addition, it was discovered that identical biological processes and pathways in the white and brown adipose tissues might be regulated by different genes. Trim30 and Ucp3 might be used as genetic markers to precisely represent the stage of obesity during the early and late stages of adipose tissue development, respectively. These results may provide a stepping-stone for future obesity-related studies.
authors.affiliation: School of Life Science, BK21 plus KNU Creative BioResearch Group, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors.forename: Hyeng-Soo
authors.initials: HS
authors.lastname: Kim
authors.affiliation: School of Life Science, BK21 plus KNU Creative BioResearch Group, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors.forename: Zae Young
authors.initials: ZY
authors.lastname: Ryoo
authors.affiliation: Korea Research Institute of Chemical Technology, Daejeon 305-600, Republic of Korea.
authors.forename: Sang Un
authors.initials: SU
authors.lastname: Choi
authors.affiliation: School of Life Science, BK21 plus KNU Creative BioResearch Group, Kyungpook National University, Daegu 702-701, Republic of Korea. Electronic address: slee@knu.ac.kr.
authors.forename: Sanggyu
authors.initials: S
authors.lastname: Lee
mesh_terms: D002001:Adipose Tissue, Brown / Q000378:metabolism*; D052436:Adipose Tissue, White / Q000378:metabolism*; D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics*; D059305:Diet, High-Fat / Q000009:adverse effects*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D007473:Ion Channels / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D024101:Mitochondrial Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009765:Obesity / Q000139:chemically induced / Q000235:genetics*; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics*; D000071247:Uncoupling Protein 3
chemical_list: D004268:DNA-Binding Proteins; D007473:Ion Channels; D024101:Mitochondrial Proteins; D014157:Transcription Factors; C055548:Trim30a protein, mouse; C000604392:UCP3 protein, human; C000604393:Ucp3 protein, mouse; D000071247:Uncoupling Protein 3
keywords: Brown adipose tissue; Epididymal fat; Interscapular brown fat; Microarray; Obesity; White adipose tissue