title: Plant mitochondrial proteomics.
abstract: Mitochondrial proteomics has significantly developed since the first plant mitochondrial proteomes were published in 2001. Many studies have added to our knowledge of the protein components that make up plant mitochondria in a wide range of species. Here we present two common and one emerging quantitative proteomic techniques that can be used to study the abundance of mitochondrial proteins. For this publication, we have described the methods as an approach to determine the amount of contamination in a mitochondrial isolation to contrast historical approaches that involved the use of use of antibodies to specific marker proteins or the measurement of activity of marker enzymes. However, these approaches could easily be adapted to carry out control versus treatment studies.
authors.affiliation: Plant Energy Biology, Australian Research Council Centre of Excellence and Centre for Comparative Analysis of Biomolecular Networks (CABiN), The University of Western Australia, Bayliss Building M316, 35 Stirling Highway, Crawley, WA, 6009, Australia.
authors.forename: Nicolas L
authors.initials: NL
authors.lastname: Taylor
authors.forename: A Harvey
authors.initials: AH
authors.lastname: Millar
mesh_terms: D002232:Carbocyanines / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005456:Fluorescent Dyes / Q000032:analysis; D007525:Isoelectric Focusing / Q000379:methods; D024101:Mitochondrial Proteins / Q000032:analysis*; D010940:Plant Proteins / Q000032:analysis*; D010944:Plants / Q000737:chemistry*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D002232:Carbocyanines; D005456:Fluorescent Dyes; D024101:Mitochondrial Proteins; D010940:Plant Proteins; D020543:Proteome