title: Cleavage pattern predicts developmental potential of day 3 human embryos produced by IVF.
abstract: Time-lapse technique provides opportunities to observe the dynamic process of human early development. Previous studies have suggested several abnormal division patterns were associated with decreased developmental potential, but no systematic results are currently available. In this study, seven abnormal division patterns were observed during early cleavage, and these had different effects on the further development potential of daughter blastomeres. According to the severity and occurrence of abnormal division patterns during the initial three cleavages, an embryo hierarchical classification model was developed and day 3 embryos were classified into six grades (from A to F). The good-quality blastocyst formation rate for these grades decreased from 70.8-3.8% (P < 0.001). In a prospective observational study, 139 IVF cycles were recruited to assess the efficiency of this classification model. In the embryos that had confirmed implantation results, the implantation rate decreased from 67.0% (Grade A) to 0% (Grade D;P < 0.001). These results indicated that cleavage patterns can predict the developmental potential of day 3 human embryos.
authors.affiliation: Institute of Reproductive and Stem Cell Engineering, Central South University, China; Key Laboratory of Reproductive and Stem Cell Engineering, Ministry of Health, China.
authors.forename: S T
authors.initials: ST
authors.lastname: Yang
authors.affiliation: Institute of Reproductive and Stem Cell Engineering, Central South University, China; Key Laboratory of Reproductive and Stem Cell Engineering, Ministry of Health, China.
authors.forename: J X
authors.initials: JX
authors.lastname: Shi
authors.affiliation: Institute of Reproductive and Stem Cell Engineering, Central South University, China; Key Laboratory of Reproductive and Stem Cell Engineering, Ministry of Health, China; Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha 410078, China.
authors.forename: F
authors.initials: F
authors.lastname: Gong
authors.affiliation: Institute of Reproductive and Stem Cell Engineering, Central South University, China; Key Laboratory of Reproductive and Stem Cell Engineering, Ministry of Health, China; Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha 410078, China.
authors.forename: S P
authors.initials: SP
authors.lastname: Zhang
authors.affiliation: Institute of Reproductive and Stem Cell Engineering, Central South University, China; Key Laboratory of Reproductive and Stem Cell Engineering, Ministry of Health, China; Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha 410078, China.
authors.forename: C F
authors.initials: CF
authors.lastname: Lu
authors.affiliation: Institute of Reproductive and Stem Cell Engineering, Central South University, China; Key Laboratory of Reproductive and Stem Cell Engineering, Ministry of Health, China; National Engineering and Research Center of Human Stem Cells, Changsha, China.
authors.forename: K
authors.initials: K
authors.lastname: Tan
authors.affiliation: Institute of Reproductive and Stem Cell Engineering, Central South University, China; Key Laboratory of Reproductive and Stem Cell Engineering, Ministry of Health, China.
authors.forename: L Z
authors.initials: LZ
authors.lastname: Leng
authors.affiliation: Institute of Reproductive and Stem Cell Engineering, Central South University, China; Key Laboratory of Reproductive and Stem Cell Engineering, Ministry of Health, China.
authors.forename: M
authors.initials: M
authors.lastname: Hao
authors.affiliation: Institute of Reproductive and Stem Cell Engineering, Central South University, China; Key Laboratory of Reproductive and Stem Cell Engineering, Ministry of Health, China.
authors.forename: H
authors.initials: H
authors.lastname: He
authors.affiliation: Institute of Reproductive and Stem Cell Engineering, Central South University, China; Key Laboratory of Reproductive and Stem Cell Engineering, Ministry of Health, China; Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha 410078, China.
authors.forename: Y F
authors.initials: YF
authors.lastname: Gu
authors.affiliation: Institute of Reproductive and Stem Cell Engineering, Central South University, China; Key Laboratory of Reproductive and Stem Cell Engineering, Ministry of Health, China; Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha 410078, China; National Engineering and Research Center of Human Stem Cells, Changsha, China.
authors.forename: G X
authors.initials: GX
authors.lastname: Lu
authors.affiliation: Institute of Reproductive and Stem Cell Engineering, Central South University, China; Key Laboratory of Reproductive and Stem Cell Engineering, Ministry of Health, China; Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha 410078, China; National Engineering and Research Center of Human Stem Cells, Changsha, China. Electronic address: linggf@hotmail.com.
authors.forename: G
authors.initials: G
authors.lastname: Lin
mesh_terms: D000328:Adult; D004622:Embryo, Mammalian / Q000166:cytology*; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D008954:Models, Biological; D011247:Pregnancy
keywords: blastocyst; cleavage; embryo; time-lapse