title: [Combined application the siRNA target for Hif-lα and Survivin gene to human NPC CNE-2 cell and its effects on the proliferation and cycle of this cell].
abstract: OBJECTIVE
  To study the combined transfection of the siRNA target for Hif-lα and Survivin gene to human NPC CNE-2 cell and its effects on the proliferation and cycle of this cell.
  
  
  METHODS
  Combined transfection of the siRNA target for Hif-lα and Survivin gene to human NPC CNE-2 cell, these plasmids were respectively transfected into the same cells. Cell proliferation was detected with MTT assay. The inhibitory effects on target genes were evaluated with RT-PCR and Western Blot at the levels of mRNA and protein, respectively.
  
  
  RESULTS
  MTT showed that CNE-2 cell proliferation in multi-gene plasmid group was more significantly inhibited than a single gene. The expression of mRNA and protein of two different genes were both decreased in HS group, and the interference effect of multiple genes was better than the single-gene(P<0.01).
  
  
  CONCLUSIONS
  HS group could restrain cell proliferation and interference the mRNA and protein expression in nasopharyngeal carcinoma CNE-2 cell, which was better than the other groups.
authors.forename: Yanxin
authors.initials: Y
authors.lastname: Ren
authors.forename: Shuping
authors.initials: S
authors.lastname: Zhang
authors.forename: Xiaojiang
authors.initials: X
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Ruimei
authors.initials: R
authors.lastname: Sun
authors.forename: Liufang
authors.initials: L
authors.lastname: Zhao
authors.forename: Lei
authors.initials: L
authors.lastname: Li
mesh_terms: D017209:Apoptosis; D002277:Carcinoma; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics*; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics*; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics*; D010957:Plasmids; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D000077022:Survivin; D014162:Transfection
chemical_list: C107489:BIRC5 protein, human; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D051034:Inhibitor of Apoptosis Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D000077022:Survivin