title: [Antibodies for detection of E/K amino acid substitution in 129 position of the survivin sequence].
abstract: Survivin is an oncofetal protein involved both in inhibiting of apoptosis and in cell cycle regulation. The functions of survivin are defined by its structural state. Due to nature polymorphism, survivin cancontain either E or K amino acid in 129 residue, and K129 is commonly acetylated. Only the protein having acetylated K129 tends to form dimeric structure. Thus, antibodies detecting the amino acid substitution can be a useful tool for structural and functional research of the protein. To obtain the antibodies specific to amino acid substitution E129/K129 peptide fragments overlapping 129 amino acid residue were synthesized, rabbits were immunized with the peptides and affinity purification of the antibodies on sepharose conjugated with the peptides was carried out. The data of ELISA and western blot showed that antibodies obtained were able to detect amino acid substitution E129/K129 in the recombinant and endogenous survivin.
authors.forename: T D
authors.initials: TD
authors.lastname: Volkova
authors.forename: E V
authors.initials: EV
authors.lastname: Askarova
authors.forename: D O
authors.initials: DO
authors.lastname: Koroev
authors.forename: A V
authors.initials: AV
authors.lastname: Kamynina
authors.forename: M P
authors.initials: MP
authors.lastname: Filatova
authors.forename: I Iu
authors.initials: IIu
authors.lastname: Iakupov
authors.forename: O M
authors.initials: OM
authors.lastname: Vol'pina
mesh_terms: D000107:Acetylation; D019943:Amino Acid Substitution / Q000235:genetics; D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D017209:Apoptosis / Q000235:genetics; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D061986:MCF-7 Cells; D010446:Peptide Fragments / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000276:immunology; D055503:Protein Multimerization; D011817:Rabbits; D013329:Structure-Activity Relationship; D000077022:Survivin
chemical_list: D000906:Antibodies; C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D010446:Peptide Fragments; D010455:Peptides; D000077022:Survivin