title: [Dynamic study on curative effect of Shuganjianpifang against hepatic fibrosis induced by CCl4 in rats].
abstract: OBJECTIVE
  To observe the dynamic effects of Shuganjianpifang (SGJPF) in preventing rat hepatic fibrosis induced by CCl4.
  
  
  METHODS
  The model rat was induced by subcutaneous injection of 50% CCl4 solution in olive oil (0.1 mL/100 g), twice a week for 12 weeks. The treatment groups were given by lavage with SGJPF and Colchicine respectively once a day for 16 weeks. After 4, 8, 12 and 16 weeks, six rats were randomly sacrificed in each group. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), and glutathione (GSH) in serum as well as superoxide dismutase (SOD) and malondialdehyde (MDA) in hapatic tissues were examined by colorimetric methods dynamically. Meanwhile, the histopathologic changes of hepatic tissues were observed by the HE stained method.
  
  
  RESULTS
  Compared with normal group, the levels of ALT and AST in serum and MDA in hepatic tissues were significantly elevated, the levels of SOD in hepatic tissues and GSH in serum were significantly decreased in model group after 4, 8, 12 and 16 weeks. Compared with model group, the levels of ALT and AST in serum and MDA in hepatic tissues were significantly reduced, the levels of SOD in hepatic tissues and GSH in serum were increased significantly after 8, 12 and 16 weeks in SGJPF group and Colchicine group. Histopathologic examination results also showed that SGJPF reduced the degree of liver fibrosis after 4, 8, 12 and 16 weeks.
  
  
  CONCLUSIONS
  Shuganjianpifang can obviously inhibit the formation of liver fibrosis at different phases in rats.
authors.forename: Hui
authors.initials: H
authors.lastname: Jiang
authors.forename: Fu-rong
authors.initials: FR
authors.lastname: Wu
authors.forename: Jia-rong
authors.initials: JR
authors.lastname: Gao
authors.forename: Jin-feng
authors.initials: JF
authors.lastname: Chen
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D002251:Carbon Tetrachloride; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000188:drug therapy*; D008315:Malondialdehyde / Q000378:metabolism; D051381:Rats; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D008315:Malondialdehyde; D002251:Carbon Tetrachloride; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D005978:Glutathione