title: Haplotype analysis of the Apolipoprotein A5 gene in Moroccan patients with the metabolic syndrome.
abstract: BACKGROUND
  In this case-control study we investigated the relative contribution of commons APOA5 polymorphisms and haplotypes to the risk of metabolic syndrome in Moroccan patients.
  
  
  METHODS
  Using the International Diabetes Federation (IDF) criteria for metabolic syndrome, the study included 176 patients and 105 controls. We genotyped APOA5 polymorphisms (-1131 T > C, c.56C > G, c.553G > T and c.1259 T > C) by PCR-RFLP analysis. The effects of APOA5 polymorphisms and constructed haplotypes on metabolic syndrome were estimated using logistic regression analyses.
  
  
  RESULTS
  The statistical analysis showed a significant association between APOA5 -1131 T > C and APOA5 c.56C > G polymorphisms with metabolic syndrome in both Codominant and Dominant models. The APOA5 -1131 T > C polymorphism was associated with increased fasting glucose (p = 0.0295) and reduced HDL levels (p = 0.0091). Carriers of the APOA5 c.56G allele had increased triglyceride levels (p = 0.0435) and waist circumference (p = 0.0122). Similarly the APOA5 1259 T > C variant was associated with increased waist circumference (p = 0.0463). The haplotypes CCGT (OR = 3.223; p = 0.00278) and CGGT (OR = 8.234; p = 0.00534) were significantly associated with susceptibility to metabolic syndrome.
  
  
  CONCLUSIONS
  Our results confirms the association of APOA5 -1131 T > C and c.56C > G variants with the predisposition to metabolic syndrome complications.
authors.affiliation: Département de Recherche Scientifique, Laboratoire de Génétique Moléculaire Humaine, Institut Pasteur du Maroc, 1, Place Louis Pasteur, 20360 Casablanca, Morocco.
authors.forename: Maria
authors.initials: M
authors.lastname: Ajjemami
authors.affiliation: Département de Recherche Scientifique, Laboratoire de Génétique Moléculaire Humaine, Institut Pasteur du Maroc, 1, Place Louis Pasteur, 20360 Casablanca, Morocco.
authors.forename: Sanaa
authors.initials: S
authors.lastname: Ouatou
authors.affiliation: Département de Recherche Scientifique, Laboratoire de Génétique Moléculaire Humaine, Institut Pasteur du Maroc, 1, Place Louis Pasteur, 20360 Casablanca, Morocco ; Univ Hassan 1, Laboratoire Agroalimentaire et Santé, 26000 Settat, Morocco.
authors.forename: Hicham
authors.initials: H
authors.lastname: Charoute
authors.affiliation: Univ Hassan 1, Laboratoire Agroalimentaire et Santé, 26000 Settat, Morocco.
authors.forename: Malika
authors.initials: M
authors.lastname: Fakiri
authors.affiliation: Laboratoire de Physiologie et Génétique Moléculaire, Faculté des sciences Ben M'Sik, Université Hassan II, Mohammedia, Morocco.
authors.forename: Houria
authors.initials: H
authors.lastname: Rhaissi
authors.affiliation: Département de Recherche Scientifique, Laboratoire de Génétique Moléculaire Humaine, Institut Pasteur du Maroc, 1, Place Louis Pasteur, 20360 Casablanca, Morocco.
authors.forename: Houda
authors.initials: H
authors.lastname: Benrahma
authors.affiliation: Département de Recherche Scientifique, Laboratoire de Génétique Moléculaire Humaine, Institut Pasteur du Maroc, 1, Place Louis Pasteur, 20360 Casablanca, Morocco.
authors.forename: Hassan
authors.initials: H
authors.lastname: Rouba
authors.affiliation: Département de Recherche Scientifique, Laboratoire de Génétique Moléculaire Humaine, Institut Pasteur du Maroc, 1, Place Louis Pasteur, 20360 Casablanca, Morocco.
authors.forename: Abdelhamid
authors.initials: A
authors.lastname: Barakat
keywords: APOA5 gene; Haplotypes; Metabolic Syndrome; Polymorphisms