title: Molecular cloning of the insulin-like growth factor 3 and difference in the expression of igf genes in orange-spotted grouper (Epinephelus coioides).
abstract: Insulin-like growth factor (Igf) is the key regulator for development, growth, and reproduction. In most vertebrate species, the Igf family has two forms: Igf1 and Igf2. A novel form of Igf, termed Igf3, was recently discovered in fish. In the present study, we isolated igf3 from the orange-spotted grouper (Epinephelus coioides). The orange-spotted grouper igf3 consisted of a full-length cDNA of 1014 nucleotides with an open reading frame (ORF) of 597 bp, encoding for proteins of 199 amino acid residues in length. Tissue distribution analysis showed that igf1 widely expressed with the highest expression in the pituitary and liver. igf2 was expressed highly in all the tissues except the olfactory bulb, while igf3 showed the highest expression in the ovary, and moderate expression in brain areas. The expression profiles of three igf genes during the ovarian development and growth hormone (Gh) and human chorionic gonadotropin (hCG) treatment were also investigated. Three igf genes exhibited different expression patterns during the ovarian development, and showed different responses to the Gh and hCG treatments, appearing to play distinct roles in ovarian development. The present study provides further evidence for the existence of an intraovarian Igf system in orange-spotted grouper.
authors.affiliation: State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and the Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China; Animal Science College, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Huirong
authors.initials: H
authors.lastname: Yang
authors.affiliation: Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China.
authors.forename: Huapu
authors.initials: H
authors.lastname: Chen
authors.affiliation: Animal Science College, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Huihong
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Animal Science College, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and the Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and the Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Xiao
authors.affiliation: State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and the Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China. Electronic address: lshuish@mail.sysu.edu.cn.
authors.forename: Shuisheng
authors.initials: S
authors.lastname: Li
authors.affiliation: State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and the Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China. Electronic address: lsszy@mail.sysu.edu.cn.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and the Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China; College of Ocean, Hainan University, Haikou 570228, China.
authors.forename: Haoran
authors.initials: H
authors.lastname: Lin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001492:Bass / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D003001:Cloning, Molecular / Q000379:methods; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013002:Somatomedins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D029941:Fish Proteins; D012333:RNA, Messenger; D013002:Somatomedins
keywords: Gh; Orange-spotted grouper; Ovarian development; hCG; igf