title: Past and future impact of next-generation sequencing in head and neck cancer.
abstract: Progress in sequencing technology is intrinsically linked to progress in understanding cancer genomics. The purpose of this review was to discuss the development from Sanger sequencing to next-generation sequencing (NGS) technology. We highlight the technical considerations for understanding reports using NGS. We discuss the findings of studies in head and neck cancer using NGS as well as The Cancer Genome Atlas. Finally we discuss future routes for research utilizing this methodology and the potential impact of this. © 2015 Wiley Periodicals, Inc. Head Neck 38: E2395-E2402, 2016.
authors.affiliation: Leeds Institute of Cancer and Pathology, Wellcome Trust Brenner Building, St. James' University Hospital, Leeds, West Yorkshire, United Kingdom.
authors.forename: Neeraj
authors.initials: N
authors.lastname: Sethi
authors.affiliation: Leeds Institute of Cancer and Pathology, Wellcome Trust Brenner Building, St. James' University Hospital, Leeds, West Yorkshire, United Kingdom.
authors.forename: Kenneth
authors.initials: K
authors.lastname: MacLennan
authors.affiliation: Leeds Institute of Cancer and Pathology, Wellcome Trust Brenner Building, St. James' University Hospital, Leeds, West Yorkshire, United Kingdom.
authors.forename: Henry M
authors.initials: HM
authors.lastname: Wood
authors.affiliation: Leeds Institute of Cancer and Pathology, Wellcome Trust Brenner Building, St. James' University Hospital, Leeds, West Yorkshire, United Kingdom.
authors.forename: Pamela
authors.initials: P
authors.lastname: Rabbitts
mesh_terms: D023281:Genomics; D006258:Head and Neck Neoplasms / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans
keywords: Sanger sequencing; genomics; head and neck cancer; human papillomavirus; next-generation sequencing