title: MicroRNA-320 inhibits cell proliferation in glioma by targeting E2F1.
abstract: MicroRNAs (miRs) are a class of small non-coding RNAs that are involved in the regulation of gene expression, and in cancer development and progression. In the present study, miR-320 expression was found to be significantly reduced in glioma tissue in comparison with that in adjacent healthy tissues. In the present study, in vitro analyses demonstrated that overexpression of miR-320 inhibited cell proliferation and metastasis, while antisense miR-320 oligonucleotides enhanced cell proliferation and migration in U251 and SHG-44 glioma cell lines, compared with that in negative control cells. Protein expression of E2F1, a cell-cycle regulator, was negatively regulated by miR-320. Therefore, the present study provides novel insights into the association between miR-320 and glioma development.
authors.affiliation: Department of Neurosurgery, Wuxi Second Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu 214002, P.R. China.
authors.forename: Ji-Yong
authors.initials: JY
authors.lastname: Sun
authors.affiliation: Department of Neurology, Shanghai Pudong Hospital, Fudan University, Pudong, Shanghai 201399, P.R. China.
authors.forename: Wei-Zhong
authors.initials: WZ
authors.lastname: Xiao
authors.affiliation: Department of Neurosurgery, Tongji Hospital, Tongji University, Shanghai 200065, P.R. China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Longhua Hospital Affiliated Shanghai Traditional Chinese Medical University, Shanghai 200032, P.R. China.
authors.forename: Yong-Qian
authors.initials: YQ
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Tongji Hospital, Tongji University, Shanghai 200065, P.R. China.
authors.forename: You-Hou
authors.initials: YH
authors.lastname: Zhu
authors.affiliation: Department of Neurosurgery, Tongji Hospital, Tongji University, Shanghai 200065, P.R. China.
authors.forename: Yi-Fang
authors.initials: YF
authors.lastname: Wu
authors.affiliation: Department of Neurosurgery, Wuxi Second Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu 214002, P.R. China.
authors.forename: Zeng-Li
authors.initials: ZL
authors.lastname: Miao
authors.affiliation: Department of Neurosurgery, Wuxi Second Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu 214002, P.R. China.
authors.forename: Yu-Chang
authors.initials: YC
authors.lastname: Lin
mesh_terms: D001483:Base Sequence; D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D050687:E2F1 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D005910:Glioma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000235:genetics / Q000378:metabolism
chemical_list: D050687:E2F1 Transcription Factor; C493753:E2F1 protein, human; C572916:MIRN320 microRNA, human; D035683:MicroRNAs; D016376:Oligonucleotides, Antisense; D008156:Luciferases