title: Expression and functions of long noncoding RNAs during human T helper cell differentiation.
abstract: Long noncoding RNAs (lncRNAs) regulate an array of biological processes in cells and organ systems. Less is known about their expression and function in lymphocyte lineages. Here we have identified >2000 lncRNAs expressed in human T-cell cultures and those that display a TH lineage-specific pattern of expression and are intragenic or adjacent to TH lineage-specific genes encoding proteins with immunologic functions. One lncRNA cluster selectively expressed by the effector TH2 lineage consists of four alternatively spliced transcripts that regulate the expression of TH2 cytokines, IL-4, IL-5 and IL-13. Genes encoding this lncRNA cluster in humans overlap the RAD50 gene and thus are contiguous with the previously described TH2 locus control region (LCR) in the mouse. Given its genomic synteny with the TH2-LCR, we refer to this lncRNA cluster as TH2-LCR lncRNA.
authors.affiliation: Department of Medicine, Vanderbilt University School of Medicine, Tennessee 37232, USA.
authors.forename: Charles F
authors.initials: CF
authors.lastname: Spurlock
authors.affiliation: Department of Medicine, Vanderbilt University School of Medicine, Tennessee 37232, USA.
authors.forename: John T
authors.initials: JT
authors.lastname: Tossberg
authors.affiliation: Department of Cancer Biology, Vanderbilt University School of Medicine, Tennessee 37213, USA.
authors.forename: Yan
authors.identifier: 0000000152523960
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Tennessee 37232, USA.
authors.forename: Sarah P
authors.initials: SP
authors.lastname: Collier
authors.affiliation: Department of Mathematics, Vanderbilt University, Tennessee 37240, USA.
authors.forename: Philip S
authors.initials: PS
authors.lastname: Crooke
authors.affiliation: 1] Department of Medicine, Vanderbilt University School of Medicine, Tennessee 37232, USA [2] Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Tennessee 37232, USA.
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Aune
mesh_terms: D017766:Acid Anhydride Hydrolases; D017398:Alternative Splicing; D002454:Cell Differentiation / Q000235:genetics* / Q000276:immunology; D047369:Chromatin Immunoprecipitation; D016207:Cytokines / Q000235:genetics* / Q000276:immunology; D045643:DNA Repair Enzymes / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D015870:Gene Expression; D005786:Gene Expression Regulation; D006801:Humans; D047468:Immunoprecipitation; D018793:Interleukin-13 / Q000235:genetics / Q000276:immunology; D015847:Interleukin-4 / Q000235:genetics / Q000276:immunology; D015848:Interleukin-5 / Q000235:genetics / Q000276:immunology; D007963:Leukocytes, Mononuclear; D019901:Locus Control Region / Q000235:genetics; D062085:RNA, Long Noncoding / Q000235:genetics*; D017423:Sequence Analysis, RNA; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology*; D058504:Th17 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*
chemical_list: D016207:Cytokines; D004268:DNA-Binding Proteins; C508598:IL4 protein, human; C508599:IL5 protein, human; D018793:Interleukin-13; D015848:Interleukin-5; D062085:RNA, Long Noncoding; D015847:Interleukin-4; D017766:Acid Anhydride Hydrolases; C112158:RAD50 protein, human; D045643:DNA Repair Enzymes