title: Aptamer-mediated nanoparticle interactions: from oligonucleotide-protein complexes to SELEX screens.
abstract: Aptamers are oligonucleotides displaying specific binding properties for a predetermined target. They can be easily immobilized on various surfaces such as nanoparticles. Functionalized particles can then be used to various aims. We took advantage of the AlphaScreen(®) technology for monitoring aptamer-mediated interactions. A particle bearing an aptamer contains a photosensitizer whereas another type of particle contains a chemiluminescer. Irradiation causes the formation of singlet oxygen species in the photosensitizer-containing bead that in turn activates the chemiluminescer. Luminescence emission can be observed if the two types of beads are in close proximity (<200 nm). This is achieved when the cognate ligand of the aptamer is grafted onto the chemiluminescer-containing bead. Using this technology we have screened oligonucleotide libraries and monitored aptamer-protein interactions. This constitutes the basis for aptamer-based analytical assays.
authors.affiliation: Novaptech, European Institute of Chemistry and Biology, Pessac, France.
authors.forename: Laetitia
authors.initials: L
authors.lastname: Evadé
authors.forename: Eric
authors.initials: E
authors.lastname: Dausse
authors.forename: Said
authors.initials: S
authors.lastname: Taouji
authors.forename: Emilie
authors.initials: E
authors.lastname: Daguerre
authors.forename: Eric
authors.initials: E
authors.lastname: Chevet
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Toulmé
mesh_terms: D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D009841:Oligonucleotides / Q000737:chemistry / Q000235:genetics; D052156:SELEX Aptamer Technique / Q000379:methods*
chemical_list: D009841:Oligonucleotides