title: TOPK is highly expressed in circulating tumor cells, enabling metastasis of prostate cancer.
abstract: Circulating tumor cells (CTCs) are important for metastasis in prostate cancer. T-LAK cell-originated protein kinase (TOPK) is highly expressed in cancer cells. Herein, we established a xenograft animal model, isolated and cultured the CTCs, and found CTCs have significantly greater migratory capacity than parental cells. TOPK is more highly expressed in the CTCs than in parental cells and is also highly expressed in the metastatic nodules caused by CTCs in mice. Knocking down TOPK decreased the migration of CTCs both in vitro and in vivo. TOPK was modulated by the PI3K/PTEN and ERK pathways during the metastasis of prostate cancer. High levels of TOPK in the tumors of patients were correlated with advanced stages of prostate cancer, especially for high-risk patients of Gleason score≥8, PSA>20ng/ml. In summary, TOPK was speculated to be one of a potential marker and therapeutic target in advanced prostate cancer.
authors.affiliation: Department of Urology, Xijing Hospital, The Fourth Military Medical University, Xian, China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Sun
authors.affiliation: Department of Epidemiology, Faculty of Preventive Medicine, The Fourth Military Medical University, Xian, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Laboratory Animal Center, The Fourth Military Medical University, Xian, Shaanxi, China.
authors.forename: Changhong
authors.initials: C
authors.lastname: Shi
authors.affiliation: Department of Pathology, Xijing Hospital, The Fourth Military Medical University, Xian, China.
authors.forename: Peizhen
authors.initials: P
authors.lastname: Hu
authors.affiliation: Department of Pathology, Xijing Hospital, The Fourth Military Medical University, Xian, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yan
authors.affiliation: Department of Pathology, Xijing Hospital, The Fourth Military Medical University, Xian, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Basic Medicine, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Qiuhong
authors.initials: Q
authors.lastname: Duan
authors.affiliation: Department of Biochemistry, Department of Basic Medicine, The Fourth Military Medical University, Xian, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Lu
authors.affiliation: PLA Lhasa General Hospital, Lhasa, China.
authors.forename: Lipeng
authors.initials: L
authors.lastname: Qin
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Basic Medicine, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Lu
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Basic Medicine, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Xiao
authors.affiliation: Department of Pathology, Xijing Hospital, The Fourth Military Medical University, Xian, China.
authors.forename: Yingmei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Basic Medicine, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhu
authors.affiliation: Department of Urology, Xijing Hospital, The Fourth Military Medical University, Xian, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Shao
mesh_terms: D000818:Animals; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D020929:Mitogen-Activated Protein Kinase Kinases / Q000096:biosynthesis*; D009361:Neoplasm Invasiveness / Q000473:pathology*; D009360:Neoplastic Cells, Circulating / Q000378:metabolism* / Q000473:pathology*; D011471:Prostatic Neoplasms / Q000473:pathology*; D023041:Xenograft Model Antitumor Assays
chemical_list: D020929:Mitogen-Activated Protein Kinase Kinases; C409022:PDZ-binding kinase
keywords: T-LAK cell-originated protein kinase (TOPK); circulating tumor cells; metastasis; prostate cancer