title: The case for primary salivary rhabdomyosarcoma.
abstract: Rhabdomyosarcomas of the parotid and submandibular glands have the histological appearance of a skeletal muscle tumor yet can be found in tissue with no striated muscular elements. We examine the potential cell-of-origin for rhabdomyosarcoma and whether salivary tumors represent primary malignancy or metastasis. We have previously established genetically engineered mouse models of rhabdomyosarcoma. In these mice, rhabdomyosarcoma is only induced when a Pax3:Foxo1 fusion oncogene is activated with concurrent loss of p53 function (for alveolar rhabdomyosarcoma) or loss of p53 function alone (for embryonal rhabdomyosarcoma) using Cre-lox technology. These mutations are only activated under the control of promoters specific for selected cell lineages, previously thought to be myogenesis-restricted. RT-PCR and immunohistochemistry for lineage-specific promoter gene products reveal these promoters are active in wild-type mouse salivary gland. Given that mouse rhabdomyosarcoma frequently originates in the salivary glands and these myogenic-related promoters are normally expressed in salivary tissue, a high likelihood exists that the salivary gland contains a cell-of-origin of this muscle-related cancer.
authors.affiliation: Department of Otolaryngology - Head and Neck Surgery, Oregon Health & Science University , Portland, OR , USA.
authors.forename: Mathew
authors.initials: M
authors.lastname: Geltzeiler
authors.affiliation: Pediatric Cancer Biology Program, Department of Pediatrics, Papé Family Pediatric Research Institute, Oregon Health & Science University , Portland, OR , USA.
authors.forename: Guangheng
authors.initials: G
authors.lastname: Li
authors.affiliation: Pediatric Cancer Biology Program, Department of Pediatrics, Papé Family Pediatric Research Institute, Oregon Health & Science University , Portland, OR , USA.
authors.forename: Jinu
authors.initials: J
authors.lastname: Abraham
authors.affiliation: Pediatric Cancer Biology Program, Department of Pediatrics, Papé Family Pediatric Research Institute, Oregon Health & Science University , Portland, OR , USA ; Children's Cancer Therapy Development Institute , Fort Collins, CO , USA.
authors.forename: Charles
authors.initials: C
authors.lastname: Keller
keywords: head and neck oncology; oncogenesis; rhabdomyosarcoma; salivary gland; salivary gland pathology; sarcoma; tumor biology