title: Anticancer Activity of the Antimicrobial Peptide Scolopendrasin VII Derived from the Centipede, Scolopendra subspinipes mutilans.
abstract: Previously, we performed de novo RNA sequencing of Scolopendra subspinipes mutilans using high-throughput sequencing technology and identified several antimicrobial peptide candidates. Among them, a cationic antimicrobial peptide, scolopendrasin VII, was selected based on its physicochemical properties, such as length, charge, and isoelectric point. Here, we assessed the anticancer activities of scolopendrasin VII against U937 and Jurkat leukemia cell lines. The results showed that scolopendrasin VII decreased the viability of the leukemia cells in MTS assays. Furthermore, flow cytometric analysis and acridine orange/ethidium bromide staining revealed that scolopendrasin VII induced necrosis in the leukemia cells. Scolopendrasin VII-induced necrosis was mediated by specific interaction with phosphatidylserine, which is enriched in the membrane of cancer cells. Taken together, these data indicated that scolopendrasin VII induced necrotic cell death in leukemia cells, probably through interaction with phosphatidylserine. The results provide a useful anticancer peptide candidate and an efficient strategy for new anticancer peptide development.
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration, Wanju 565-851, Republic of Korea.
authors.forename: Joon Ha
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration, Wanju 565-851, Republic of Korea.
authors.forename: In-Woo
authors.initials: IW
authors.lastname: Kim
authors.affiliation: Center for Liver Cancer, National Cancer Center, Goyang 410-769, Republic of Korea.
authors.forename: Sang-Hee
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration, Wanju 565-851, Republic of Korea.
authors.forename: Mi-Ae
authors.initials: MA
authors.lastname: Kim
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration, Wanju 565-851, Republic of Korea.
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Yun
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration, Wanju 565-851, Republic of Korea.
authors.forename: Sung-Hee
authors.initials: SH
authors.lastname: Nam
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration, Wanju 565-851, Republic of Korea.
authors.forename: Mi-Young
authors.initials: MY
authors.lastname: Ahn
authors.affiliation: Ilsong Institute of Life Science, Hallym University, Anyang 431-060, Republic of Korea.
authors.forename: Dongchul
authors.initials: D
authors.lastname: Kang
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration, Wanju 565-851, Republic of Korea.
authors.forename: Jae Sam
authors.initials: JS
authors.lastname: Hwang
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000302:isolation & purification* / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000187:drug effects* / Q000502:physiology; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D000077743:Diterpene Alkaloids; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D006801:Humans; D019169:Jurkat Cells; D010718:Phosphatidylserines / Q000378:metabolism; D020298:U937 Cells
chemical_list: D023181:Antimicrobial Cationic Peptides; D000970:Antineoplastic Agents; D000077743:Diterpene Alkaloids; D004365:Drugs, Chinese Herbal; D010718:Phosphatidylserines; C574917:scolopendra alkaloids
keywords: Anticancer activity; Antimicrobial peptide; Necrosis; Phosphatidylserine; Scolopendrasin VII