title: REVEILLE1 promotes NADPH: protochlorophyllide oxidoreductase A expression and seedling greening in Arabidopsis.
abstract: Chlorophyll biosynthesis plays a crucial role in the greening process and survival of etiolated seedlings and yet the mechanism underlying the regulation of this process is poorly understood. Upon light stimulation, NADPH: protochlorophyllide oxidoreductase (POR) catalyzes the reduction of protochlorophyllide (Pchlide) to chlorophyllide. Whereas this represents a key step in the chlorophyll biosynthetic pathway, the regulation of POR remains largely unknown. Three POR isoforms exist in Arabidopsis thaliana, i.e., PORA, PORB, and PORC. In this study, we identified a transcription factor, REVEILLE1 (RVE1), that binds directly to the PORA promoter through the EE-box cis-regulatory element. Analysis of PORA expression in RVE1 loss-of-function (rve1) and overexpression (RVE1-OX) Arabidopsis plants showed that RVE1 positively regulates the transcription of PORA. We found that Pchlide levels were reduced in RVE1-OX seedlings. Furthermore, rve1 etiolated seedlings had lower greening rates than the wild type when exposed to light, whereas RVE1-OX seedlings had higher greening rates. In addition, when etiolated seedlings were exposed to light, RVE1-OX plants had less reactive oxygen species (ROS) accumulation and cell death than the wild type, and had reduced levels of ROS-responsive gene expression. Taken together, our study reveals an important role for RVE1 in regulating chlorophyll biosynthesis and promoting seedling greening during early plant growth and development.
authors.affiliation: Key Laboratory of Photobiology, Institute of Botany, The Chinese Academy of Sciences, Beijing, 100093, China.|University of the Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Xu
authors.affiliation: Key Laboratory of Photobiology, Institute of Botany, The Chinese Academy of Sciences, Beijing, 100093, China.|University of the Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Guo
authors.affiliation: Key Laboratory of Photobiology, Institute of Botany, The Chinese Academy of Sciences, Beijing, 100093, China.|University of the Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Photobiology, Institute of Botany, The Chinese Academy of Sciences, Beijing, 100093, China.|Institute of Genetics and Developmental Biology, The Chinese Academy of Sciences, Beijing, 1000, China.
authors.forename: Dongqin
authors.initials: D
authors.lastname: Chen
authors.affiliation: Key Laboratory of Photobiology, Institute of Botany, The Chinese Academy of Sciences, Beijing, 100093, China.|University of the Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Photobiology, Institute of Botany, The Chinese Academy of Sciences, Beijing, 100093, China. rclin@ibcas.ac.cn.
authors.forename: Rongcheng
authors.initials: R
authors.lastname: Lin
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000254:growth & development / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002734:Chlorophyll / Q000378:metabolism; D008027:Light; D009249:NADP / Q000378:metabolism; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000378:metabolism*; D011521:Protochlorophyllide / Q000378:metabolism*; D036226:Seedlings / Q000201:enzymology / Q000254:growth & development / Q000528:radiation effects; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; C545814:REVEILLE1 protein, Arabidopsis; D014157:Transcription Factors; D002734:Chlorophyll; D011521:Protochlorophyllide; D009249:NADP; D044925:Oxidoreductases Acting on CH-CH Group Donors; C023886:protochlorophyllide reductase
keywords: Chlorophyll biosynthesis; POR; RVE1; Seedling greening