title: Genomic and non-genomic effects of progesterone on prostaglandin (PG) F2a and PGE2 production in the bovine endometrium.
abstract: Progesterone (P4) acts through different actuating pathways called genomic and non-genomic pathways. Here we investigated whether P4 regulates prostaglandin (PG) F2a (PGF) and PGE2 production in bovine endometrium through different pathways. Cultured endometrial cells were exposed to P4 for a short time (5-20 min) or bovine serum albumin (BSA)-conjugated P4 (P4-BSA) for 24 h. Progesterone treatment for 24 h stimulated PGE2 production in epithelial cells, but suppressed both PGF and PGE2 production and the expression of PG-metabolising enzymes including phospholipase A2 (PLA2) and cyclooxygenase-2 (COX2) in stromal cells. Short-term (5-20 min) P4 treatment did not affect PLA2 or COX2 transcript levels in either cell type. P4-BSA increased PGF and PGE2 production only in epithelial cells. Nuclear P4 receptor mRNA expression in endometrium was higher at the follicular phase than at the early- to mid-luteal stages, whereas membrane P4 receptor mRNA expression did not change throughout the oestrous cycle. The overall results suggest that P4 controls PG production by inhibiting enzymes via a genomic pathway and by stimulating signal transduction via a non-genomic pathway. Consequently, P4 may protect the corpus luteum by attenuating PGF production in stromal cells and by increasing PGE2 secretion from epithelial cells.
authors.affiliation: Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University, Okayama 700-8530, Japan.
authors.forename: Mariko
authors.initials: M
authors.lastname: Kuse
authors.affiliation: Reproductive Biology Research Unit, National Institute of Agrobiological Sciences, Ibaraki 305-0901, Japan.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Sakumoto
authors.affiliation: Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University, Okayama 700-8530, Japan.|Corresponding author. Email: kokuda@okayama-u.ac.jp.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Okuda
keywords: cows; luteolysis; steroid hormone; uterus