title: The analysis of sialylation, N-glycan branching, and expression of O-glycans in seminal plasma of infertile men.
abstract: Carbohydrates are known to mediate some events involved in successful fertilization. Although some studies on the glycosylation of seminal plasma proteins are available, the total glycan profile was rarely analyzed as a feature influencing fertilization potential. In this work we aimed to compare some glycosylation traits in seminal plasma glycoproteins of fertile and infertile men. The following findings emerge from our studies: (1) in human seminal plasma the presence and alterations of O-linked glycans were observed; (2) the expression of SNA-reactive sialic acid significantly differs between asthenozoospermia and both normozoospermic (fertile and infertile) groups; (3) the expression of PHA-L-reactive highly branched N-glycans was significantly lower in oligozoospermic patients than in both normozoospermic groups. Indication of the appropriate lectins that would enable the possibly precise determination of the glycan profile seems to be a good supplement to mass spectrum analysis. Extension of the lectin panel is useful for the further research.
authors.affiliation: Department of Chemistry and Immunochemistry, Wrocław Medical University, Bujwida 44a, 50-345 Wrocław, Poland.
authors.forename: Ewa M
authors.initials: EM
authors.lastname: Kratz
authors.affiliation: Department of Chemistry and Immunochemistry, Wrocław Medical University, Bujwida 44a, 50-345 Wrocław, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Kałuża
authors.affiliation: 2nd Department and Clinic of Gynaecology, Obstetrics and Neonatology, Wrocław Medical University, Academic Hospital, Borowska 213, 50-556 Wrocław, Poland.
authors.forename: Mariusz
authors.initials: M
authors.lastname: Zimmer
authors.affiliation: Department of Chemistry and Immunochemistry, Wrocław Medical University, Bujwida 44a, 50-345 Wrocław, Poland.
authors.forename: Mirosława
authors.initials: M
authors.lastname: Ferens-Sieczkowska
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D006031:Glycosylation; D006801:Humans; D007248:Infertility, Male / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D019158:N-Acetylneuraminic Acid / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*; D012661:Semen / Q000378:metabolism*
chemical_list: D037102:Lectins; D011134:Polysaccharides; D019158:N-Acetylneuraminic Acid