title: Arpin contributes to bacterial translocation and development of severe acute pancreatitis.
abstract: OBJECTIVE
  To assess the impact of Arpin protein and tight junction (TJ) proteins in the intestinal mucosa on bacterial translocation in patients with severe acute pancreatitis (SAP).
  
  
  METHODS
  Fifty SAP patients were identified as study objects and then classified into two groups according to the presence of bacterial translocation (BT) in the blood [i.e., BT(+) and BT(-)]. Twenty healthy individuals were included in the control group. BT was analyzed by polymerase chain reaction, colonic mucosal tissue was obtained by endoscopy and the expression of TJ proteins and Arpin protein was determined using immunofluorescence and western blotting.
  
  
  RESULTS
  Bacterial DNA was detected in the peripheral blood of 62.0% of patients (31/50) with SAP. The expression of TJ proteins in SAP patients was lower than that in healthy controls. In contrast, Arpin protein expression in SAP patients was higher than in healthy controls (0.38 ± 0.19 vs 0.28 ± 0.16, P < 0.05). Among SAP patients, those positive for BT showed a higher level of claudin-2 expression (0.64 ± 0.27 vs 0.32 ± 0.21, P < 0.05) and a lower level of occludin (OC) (0.61 ± 0.28 vs 0.73 ± 0.32, P < 0.05) and zonula occludens-1 (0.42 ± 0.26 vs 0.58 ± 0.17, P = 0.038) expression in comparison with BT (-) patients. Moreover, the level of Arpin expression in BT (+) patients was higher than in BT (-) patients (0.61 ± 0.28 vs 0.31 ± 0.24, P < 0.05).
  
  
  CONCLUSIONS
  Arpin protein affects the expression of tight junction proteins and may have an impact on BT. These results contribute to a better understanding of the factors involved in bacterial translocation during acute pancreatitis.
authors.affiliation: Wen-Sheng Deng, Jiang Wang, Hong-Mei Zheng, Dian-Liang Zhang, Center of Colon and Rectum, Qingdao Municipal Hospital, Qingdao University, Qingdao 266011, Shandong Province, China.
authors.forename: Wen-Sheng
authors.initials: WS
authors.lastname: Deng
authors.affiliation: Wen-Sheng Deng, Jiang Wang, Hong-Mei Zheng, Dian-Liang Zhang, Center of Colon and Rectum, Qingdao Municipal Hospital, Qingdao University, Qingdao 266011, Shandong Province, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Wen-Sheng Deng, Jiang Wang, Hong-Mei Zheng, Dian-Liang Zhang, Center of Colon and Rectum, Qingdao Municipal Hospital, Qingdao University, Qingdao 266011, Shandong Province, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Ju
authors.affiliation: Wen-Sheng Deng, Jiang Wang, Hong-Mei Zheng, Dian-Liang Zhang, Center of Colon and Rectum, Qingdao Municipal Hospital, Qingdao University, Qingdao 266011, Shandong Province, China.
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Zheng
authors.affiliation: Wen-Sheng Deng, Jiang Wang, Hong-Mei Zheng, Dian-Liang Zhang, Center of Colon and Rectum, Qingdao Municipal Hospital, Qingdao University, Qingdao 266011, Shandong Province, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Wang
authors.affiliation: Wen-Sheng Deng, Jiang Wang, Hong-Mei Zheng, Dian-Liang Zhang, Center of Colon and Rectum, Qingdao Municipal Hospital, Qingdao University, Qingdao 266011, Shandong Province, China.
authors.forename: Su
authors.initials: S
authors.lastname: Wang
authors.affiliation: Wen-Sheng Deng, Jiang Wang, Hong-Mei Zheng, Dian-Liang Zhang, Center of Colon and Rectum, Qingdao Municipal Hospital, Qingdao University, Qingdao 266011, Shandong Province, China.
authors.forename: Dian-Liang
authors.initials: DL
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D018988:Bacterial Translocation; D015415:Biomarkers / Q000097:blood; D002352:Carrier Proteins / Q000032:analysis*; D016022:Case-Control Studies; D003106:Colon / Q000737:chemistry* / Q000382:microbiology; D004269:DNA, Bacterial / Q000097:blood; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000737:chemistry* / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D019283:Pancreatitis, Acute Necrotizing / Q000097:blood / Q000378:metabolism* / Q000382:microbiology; D011446:Prospective Studies; D012720:Severity of Illness Index; D062725:Tight Junction Proteins / Q000032:analysis*; D015854:Up-Regulation
chemical_list: D015415:Biomarkers; D002352:Carrier Proteins; D004269:DNA, Bacterial; D062725:Tight Junction Proteins; C000604273:arpin protein, human
keywords: Arpin; Bacterial translocation; Epithelium; Intestinal epithelial barrier; Severe acute pancreatitis; Tight junction proteins