title: An aptamer-based signal-on bio-assay for sensitive and selective detection of Kanamycin A by using gold nanoparticles.
abstract: In this study, a simple and sensitive aptamer-based fluorescence method for the detection of Kanamycin A by using gold nanoparticles (AuNPs) has been developed. In this assay, AuNPs were utilized as DNA nanocarrier as well as efficient fluorescence quencher. In the absence of Kanamycin A, dye-labeled aptamer could be adsorbed onto the surface of AuNPs and the fluorescence signal was quenched. In the presence of Kanamycin A, the specific binding between dye-labeled aptamer and its target induced the formation of rigid structure, which led to dye-labeled aptamer releasing from the surface of AuNPs and the fluorescence intensity was recovered consequently. Under optimum conditions, calibration modeling showed that the analytical linear range covered from 0.8nM to 350nM and the detection limit of 0.3nM was realized successfully. This proposed bio-assay also showed high selectivity over other antibiotics. Meanwhile, this strategy was further used to determine the concentrations of Kanamycin A in milk sample with satisfying results.
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, PR China. Electronic address: zhaohui.li@zzu.edu.cn.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Li
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, PR China.
authors.forename: Jia
authors.initials: J
authors.lastname: Ge
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, PR China.
authors.forename: Ran
authors.initials: R
authors.lastname: Yang
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, PR China; School of Chemistry & Chemical Engineering, Henan University of Technology, Zhengzhou 450001, PR China. Electronic address: qulingbo@zzu.edu.cn.
authors.forename: Ling-Bo
authors.initials: LB
authors.lastname: Qu
authors.affiliation: Institute of Quality Standard and Testing Technology for Agroproducts, Henan Academy of Agricultural Science, Zhengzhou 450002, PR China.
authors.forename: Hong-Qi
authors.initials: HQ
authors.lastname: Wang
authors.affiliation: Institute of Quality Standard and Testing Technology for Agroproducts, Henan Academy of Agricultural Science, Zhengzhou 450002, PR China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001681:Biological Assay / Q000379:methods*; D015374:Biosensing Techniques / Q000379:methods*; D005453:Fluorescence; D006046:Gold / Q000737:chemistry*; D007612:Kanamycin / Q000032:analysis*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D012680:Sensitivity and Specificity; D059629:Signal-To-Noise Ratio
chemical_list: D052157:Aptamers, Nucleotide; D007612:Kanamycin; D006046:Gold
keywords: Aptamer; Fluorescence; Gold nanoparticles; Kanamycin A