title: Tumor suppression function of FoxD3 in lung cancer.
abstract: BACKGROUND
  The transcription factor forkhead box D3 (FoxD3) is a forkhead protein and serves as a transcriptional repressor. The functions of FoxD3 in embryogenesis and in the development of neural crest cells have been diversely studied. Its role associated with tumor development has attracted attention just in recent years. However, little is known about its regulation and functions in the tumorigenesis and progression of lung cancer.
  
  
  METHODS
  We did a pioneer study to identify the function of FoxD3 in lung cancer by knocking down FoxD3 expression in lung adenocarcinoma cell lines.
  
  
  RESULTS
  The result showed that silencing FoxD3 in lung cancer cell lines stimulated cell growth and inhibited cell apoptosis. Through quantified PCR analysis, we observed marked gene expression increase of insulin-like growth factor receptor 1 and BRAF under the condition of FoxD3 silencing. In contrast, cell cycle negative regulator P21 expression decreased after FoxD3 silencing.
  
  
  CONCLUSIONS
  Our study is the first to identify the tumor suppressor function of FoxD3 in lung cancer.
authors.affiliation: Department of Pathology, Zhejiang Cancer Hospital, NO. 38 Guangji Road, Hangzhou, 310022, China.
authors.forename: C
authors.initials: C
authors.lastname: Wang
authors.affiliation: No. 2 Hospital Affiliated To Medical College of Zhejiang, NO. 88 Jiefang Road, Hangzhou, 310009, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Pathology, Zhejiang Cancer Hospital, NO. 38 Guangji Road, Hangzhou, 310022, China. daihlzhj@163.com.
authors.forename: W
authors.initials: W
authors.lastname: Dai
mesh_terms: D000072283:A549 Cells; D000230:Adenocarcinoma / Q000235:genetics*; D000077192:Adenocarcinoma of Lung; D000818:Animals; D017209:Apoptosis / Q000276:immunology; D049109:Cell Proliferation / Q000235:genetics; D051858:Forkhead Transcription Factors / Q000235:genetics*; D055785:Gene Knockdown Techniques; D016147:Genes, Tumor Suppressor; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*
chemical_list: C503514:FOXD3 protein, human; D051858:Forkhead Transcription Factors
keywords: Apoptosis; FoxD3; Lung cancer; Proliferation