title: An inverse association of Helicobacter pylori infection with oral squamous cell carcinoma.
abstract: BACKGROUND
  Few studies have focused on the relationship between Helicobacter pylori (H. pylori) infection and oral diseases. In this study, we explored the correlation between H. pylori infection and oral squamous cell carcinoma (OSCC).
  
  
  METHODS
  A total of 68 patients with OSCC and 104 age- and sex- matched healthy control subjects were retrospectively enrolled in this study. The H. pylori immunoglobin (Ig) G antibodies in serum were detected by enzyme-linked immunosorbent assay (ELISA) method to assess the status of H. pylori infection of our study sample. Polymerase chain reaction (PCR) was also employed using H. pylori genus-specific 16S rRNA primers in fasting blood, and OSCC specimens were analyzed by histochemical stain of each enrolled subject. The strength of correlation between H. pylori and the development of OSCC was estimated by Spearman's correlation coefficient.
  
  
  RESULTS
  According to the three methods for detecting prevalence of H. pylori infection in the patients with OSCC, it was statistically lower than that in the healthy controls (35.3% vs. 54.8%, P = 0.012). An inverse correlation was observed between H. pylori infection and OSCC development (Spearman's correlation coefficient = -0.191, P = 0.012). In stratification analysis, we also found a statistical association between H. pylori infection and OSCC in the subpopulation with age ≥ 60 years (P = 0.037).
  
  
  CONCLUSIONS
  Our findings suggested that H. pylori infection may be negatively related to OSCC. A reverse association of H. pylori infection with OSCC risk in the subpopulation with age ≥ 60 years was also found.
authors.affiliation: Tumor Etiology and Screening Department of Cancer Institute and General Surgery, the First Affiliated Hospital of China Medical University, Liaoning Provincial Education Department, Shenyang, China.|Key Laboratory of Cancer Etiology and Prevention (China Medical University), Liaoning Provincial Education Department, Shenyang, China.|Department of Oral and Maxillofacial Surgery, Shengjing Hospital, China Medical University, Shengyang City, Liaoning Province, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Meng
authors.affiliation: Department of Oral and Maxillofacial Surgery, Shengjing Hospital, China Medical University, Shengyang City, Liaoning Province, China.
authors.forename: Qiuxu
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Tumor Etiology and Screening Department of Cancer Institute and General Surgery, the First Affiliated Hospital of China Medical University, Liaoning Provincial Education Department, Shenyang, China.|Key Laboratory of Cancer Etiology and Prevention (China Medical University), Liaoning Provincial Education Department, Shenyang, China.
authors.forename: Caiyun
authors.initials: C
authors.lastname: He
authors.affiliation: Tumor Etiology and Screening Department of Cancer Institute and General Surgery, the First Affiliated Hospital of China Medical University, Liaoning Provincial Education Department, Shenyang, China.|Key Laboratory of Cancer Etiology and Prevention (China Medical University), Liaoning Provincial Education Department, Shenyang, China.
authors.forename: Moye
authors.initials: M
authors.lastname: Chen
authors.affiliation: Tumor Etiology and Screening Department of Cancer Institute and General Surgery, the First Affiliated Hospital of China Medical University, Liaoning Provincial Education Department, Shenyang, China.|Key Laboratory of Cancer Etiology and Prevention (China Medical University), Liaoning Provincial Education Department, Shenyang, China.
authors.forename: Jingwei
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Oral and Maxillofacial Surgery, Shengjing Hospital, China Medical University, Shengyang City, Liaoning Province, China.
authors.forename: Weixian
authors.initials: W
authors.lastname: Liu
authors.affiliation: Tumor Etiology and Screening Department of Cancer Institute and General Surgery, the First Affiliated Hospital of China Medical University, Liaoning Provincial Education Department, Shenyang, China.|Key Laboratory of Cancer Etiology and Prevention (China Medical University), Liaoning Provincial Education Department, Shenyang, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial / Q000097:blood; D002294:Carcinoma, Squamous Cell / Q000276:immunology / Q000382:microbiology* / Q000473:pathology; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006258:Head and Neck Neoplasms / Q000276:immunology / Q000382:microbiology* / Q000473:pathology; D016481:Helicobacter Infections / Q000276:immunology / Q000473:pathology*; D016480:Helicobacter pylori / Q000302:isolation & purification*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000276:immunology / Q000382:microbiology* / Q000473:pathology; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000097:blood; D012307:Risk Factors; D000077195:Squamous Cell Carcinoma of Head and Neck
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D012336:RNA, Ribosomal, 16S
keywords: Helicobacter pylori; PCR; enzyme-linked immunosorbent assay; oral cancer; oral squamous cell carcinoma