title: Effects of low dose T-2 toxin on secretion of gonadotropin-releasing hormone in the immortalized hypothalamic GT1-7 cell line.
abstract: T-2 toxin is one form of trichothecenes, which contaminate crops and feedstuff commonly, and has a wide variety of toxic effects in human and animals, but little is known regarding its role on reproductive function and puberty development. Previous studies have reported that reproductive capacity is attained at puberty triggered by gonadotropin-releasing hormone (GnRH) pulse secretion, and kisspeptins/GPR54 signaling pathway play a central role in the activation of GnRH neurons. For our study, T-2 toxin was tested for its effects on the secretion of GnRH in GT1-7 cells, an immortalized hypothalamic neuron cell line, which could synthesize and secrete GnRH. GT1-7 cells were treated with low doses of T-2 toxin in dose-response assays, with and without kisspeptins, and the levels of GnRH were quantified in each treatment. Furthermore, western blot analysis was used to detect the proteins expression in kisspeptins/GPR54 signaling pathway. The present study demonstrated that low dose T-2 toxin stimulate GnRH secretion and alter the expression of associated proteins in GT1-7 cells in vitro, and the activation of GT1-7 cells response to T- 2 toxin was increased after pretreatment with kisspeptins.
authors.affiliation: Department of Nutrition and Food Safety, School of Public Heath, Jilin University, 1163 Xin Min Street, Changchun 130021, China; Evaluation and Research Center for Toxicology, Institute of Disease Control and Prevention, Academy of Military Medical Sciences, 20 Dongdajie Rd, Fengtai District, Beijing 100071, China.
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Evaluation and Research Center for Toxicology, Institute of Disease Control and Prevention, Academy of Military Medical Sciences, 20 Dongdajie Rd, Fengtai District, Beijing 100071, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Evaluation and Research Center for Toxicology, Institute of Disease Control and Prevention, Academy of Military Medical Sciences, 20 Dongdajie Rd, Fengtai District, Beijing 100071, China.
authors.forename: Yimei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Evaluation and Research Center for Toxicology, Institute of Disease Control and Prevention, Academy of Military Medical Sciences, 20 Dongdajie Rd, Fengtai District, Beijing 100071, China.
authors.forename: Ruiqin
authors.initials: R
authors.lastname: Wu
authors.affiliation: Evaluation and Research Center for Toxicology, Institute of Disease Control and Prevention, Academy of Military Medical Sciences, 20 Dongdajie Rd, Fengtai District, Beijing 100071, China.
authors.forename: Chenfeng
authors.initials: C
authors.lastname: Hua
authors.affiliation: Evaluation and Research Center for Toxicology, Institute of Disease Control and Prevention, Academy of Military Medical Sciences, 20 Dongdajie Rd, Fengtai District, Beijing 100071, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Nutrition and Food Safety, School of Public Heath, Jilin University, 1163 Xin Min Street, Changchun 130021, China. Electronic address: liuya@jlu.edu.cn.
authors.forename: Ya
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Evaluation and Research Center for Toxicology, Institute of Disease Control and Prevention, Academy of Military Medical Sciences, 20 Dongdajie Rd, Fengtai District, Beijing 100071, China. Electronic address: pengsq@hotmail.com.
authors.forename: Shuangqing
authors.initials: S
authors.lastname: Peng
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism; D059648:Kisspeptins / Q000494:pharmacology; D051379:Mice; D014176:Protein Biosynthesis / Q000187:drug effects; D012098:Reproduction / Q000187:drug effects; D013605:T-2 Toxin / Q000494:pharmacology*
chemical_list: D059648:Kisspeptins; D007987:Gonadotropin-Releasing Hormone; D013605:T-2 Toxin
keywords: GT1-7 cells; GnRH; Kisspeptins; T-2 toxin