title: [Rapid determination of eight organic acids in plant tissue by sequential extraction and high performance liquid chromatography].
abstract: A sequential extraction method was developed to determine different forms of oxalate and seven oxalate-metabolism-related organic acids (glyoxylic acid, tartaric acid, glycolic acid, malic acid, acetic acid, citric acid, succinic acid) in plant tissue. The ultra-pure water was used as the extraction medium to obtain water-soluble oxalic acid and the other seven water-soluble organic acids. After the extraction of the water-soluble organic acids, the residues were extracted by dilute hydrochloric acid successively to get the acid-soluble oxalate which entered the liquid phase. A Hypersil ODS column was used with 5 mmol/L potassium dihydrogen phosphate buffer solution (pH 2. 8) as the mobile phase. The diode array detector was set at 210 nm and the column temperature at 30 °C with the injection volume of 5 µL. The flow rate was controlled at different times which allowed a good and rapid separation of the organic acids and hydrochloric acid. Under these conditions, the linear ranges of the method were 1-2000 mg/L for oxalic acid, 25-2,000 mg/L for acetic acid, and 10-2,000 mg/L for glyoxylic acid, tartaric acid, glycolic acid, malic acid, citric acid and succinic acid, with the correlation coefficients of the eight organic acids ≥ 0. 9996. The average recoveries of the eight organic acids in leaves and roots were 93. 5%-104. 4% and 85. 3%-105. 4% with RSDs of 0. 15% -2.43% and 0. 31%-2. 9% (n=7), respectively. The limits of detection ranged from 1 to 10 ng (S/N=3). The results indicated that the method is accurate, rapid and reproducible for the determination of organic acids in plant samples.
authors.forename: Tianzhi
authors.initials: T
authors.lastname: Huang
authors.forename: Shijie
authors.initials: S
authors.lastname: Wang
authors.lastname: Liu Xiuming
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Yanyou
authors.initials: Y
authors.lastname: Wu
authors.lastname: Luo Xuqiang
mesh_terms: D019342:Acetic Acid; D000143:Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D008293:Malates; D009930:Organic Chemicals; D019815:Oxalic Acid; D010944:Plants / Q000737:chemistry*; D013644:Tartrates
chemical_list: D000143:Acids; D008293:Malates; D009930:Organic Chemicals; D013644:Tartrates; C030298:malic acid; D019815:Oxalic Acid; D019342:Acetic Acid; C029768:tartaric acid