title: Interactions of the antiviral and antiparkinson agent amantadine with lipid membranes and human erythrocytes.
abstract: Aimed to better understand the molecular mechanisms of its interactions with cell membranes, human erythrocyte and molecular models of the red cell membrane were utilized. The latter consisted of bilayers of dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylethanolamine (DMPE), representative of phospholipid classes located in the outer and inner monolayers of the human erythrocyte membrane, respectively. The capacity of amantadine to perturb the bilayer structures of DMPC and DMPE was evaluated by X-ray diffraction, fluorescence spectroscopy and differential scanning calorimetry (DSC). In an attempt to further elucidate its effects on cell membranes, the present work also examined amantadine influence on the morphology of intact human erythrocytes by means of scanning electron microscopy (SEM). Results indicated that amantadine induced morphological changes to human erythrocytes and interacted in a concentration-dependent manner with DMPC bilayers in contrast to DMPE that was hardly affected by the presence of the drug.
authors.affiliation: Faculty of Chemical Sciences, University of Concepción, Concepción, Chile. Electronic address: msuwalsk@udec.cl.
authors.forename: Mario
authors.initials: M
authors.lastname: Suwalsky
authors.affiliation: Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland; Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Jemiola-Rzeminska
authors.affiliation: Faculty of Biological Sciences, University of Concepción, Concepción, Chile.
authors.forename: Mariella
authors.initials: M
authors.lastname: Altamirano
authors.affiliation: Faculty of Biological Sciences, University of Concepción, Concepción, Chile.
authors.forename: Fernando
authors.initials: F
authors.lastname: Villena
authors.affiliation: Faculty of Medicine, P. Catholic University of Chile, Santiago, Chile.
authors.forename: Nathan
authors.initials: N
authors.lastname: Dukes
authors.affiliation: Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland; Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland.
authors.forename: Kazimierz
authors.initials: K
authors.lastname: Strzalka
mesh_terms: D000547:Amantadine / Q000737:chemistry* / Q000494:pharmacology*; D000978:Antiparkinson Agents / Q000737:chemistry* / Q000494:pharmacology; D000998:Antiviral Agents / Q000737:chemistry* / Q000494:pharmacology; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D008958:Models, Molecular; D015394:Molecular Structure; D010714:Phosphatidylethanolamines / Q000737:chemistry; D013329:Structure-Activity Relationship
chemical_list: D000978:Antiparkinson Agents; D000998:Antiviral Agents; D008051:Lipid Bilayers; D010714:Phosphatidylethanolamines; D000547:Amantadine; D004134:Dimyristoylphosphatidylcholine; C018525:1,2-dimyristoylphosphatidylethanolamine
keywords: Amantadine; Erythrocyte membrane; Phospholipid bilayer