title: Gene- and stem cell-based therapeutics for cartilage regeneration and repair.
abstract: Cell-based regeneration of damaged or diseased articular cartilage still faces significant clinical challenge due to inadequate environmental regulation of stem cell proliferation and chondrogenic differentiation. The role of insulin-like growth factor in critical steps of human bone marrow-derived mesenchymal stem cell chondrogenesis has potential in optimizing the therapeutic use of mesenchymal stem cells in cartilage disorders. In addition to the previously described benefits of recombinant adeno-associated viral vector for in vivo gene therapy, demonstrated by Frisch and colleagues, such vector is also a safe and efficient delivery system for the genetic modification of human bone marrow-derived mesenchymal stem cells via ex vivo insulin-like growth factor 1 gene transfer, so that implanted mesenchymal stem cells continuously release a therapeutic level of insulin-like growth factor 1 to achieve sustained mesenchymal stem cell chondrogenesis for cartilage regeneration.
authors.affiliation: Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, 450 Technology Drive, Suite 216, Pittsburgh, PA, 15219, USA. yingtangw@yahoo.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, 450 Technology Drive, Suite 216, Pittsburgh, PA, 15219, USA. bingwang@pitt.edu.
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
mesh_terms: D002454:Cell Differentiation / Q000235:genetics*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D011498:Protein Precursors / Q000378:metabolism*; D019076:Transgenes
chemical_list: D011498:Protein Precursors; D007334:Insulin-Like Growth Factor I