title: Multiple antibiotic susceptibility of polyphosphate kinase mutants (ppk1 and ppk2) from Pseudomonas aeruginosa PAO1 as revealed by global phenotypic analysis.
abstract: BACKGROUND
  Pseudomonas aeruginosa is known to be a multidrug resistant opportunistic pathogen. Particularly, P. aeruginosa PAO1 polyphosphate kinase mutant (ppk1) is deficient in motility, quorum sensing, biofilm formation and virulence.
  
  
  RESULTS
  By using Phenotypic Microarrays (PM) we analyzed near 2000 phenotypes of P. aeruginosa PAO1 polyP kinase mutants (ppk1 and ppk2). We found that both ppk mutants shared most of the phenotypic changes and interestingly many of them related to susceptibility toward numerous and different type of antibiotics such as Ciprofloxacin, Chloramphenicol and Rifampicin.
  
  
  CONCLUSIONS
  Combining the fact that ppk1 mutants have reduced virulence and are more susceptible to antibiotics, polyP synthesis and particularly PPK1, is a good target for the design of molecules with anti-virulence and anti-persistence properties.
authors.affiliation: Systems Microbiology Laboratory, Department of Biology, Faculty of Science, University of Chile, Las Palmeras 3425, Ñuñoa, Santiago, Chile. javiera.o.s@gmail.com.
authors.forename: Javiera
authors.initials: J
authors.lastname: Ortiz-Severín
authors.affiliation: Systems Microbiology Laboratory, Department of Biology, Faculty of Science, University of Chile, Las Palmeras 3425, Ñuñoa, Santiago, Chile. mvaras26@gmail.com.
authors.forename: Macarena
authors.initials: M
authors.lastname: Varas
authors.affiliation: Systems Microbiology Laboratory, Department of Biology, Faculty of Science, University of Chile, Las Palmeras 3425, Ñuñoa, Santiago, Chile. catalina.btoncio@gmail.com.
authors.forename: Catalina
authors.initials: C
authors.lastname: Bravo-Toncio
authors.affiliation: Bacterial Communication Laboratory, Department of Biology, Faculty of Science, University of Chile, Las Palmeras 3425, Ñuñoa, Santiago, Chile. nguilian@uchile.cl.
authors.forename: Nicolás
authors.initials: N
authors.lastname: Guiliani
authors.affiliation: Systems Microbiology Laboratory, Department of Biology, Faculty of Science, University of Chile, Las Palmeras 3425, Ñuñoa, Santiago, Chile. fpchavez@uchile.cl.
authors.forename: Francisco P
authors.initials: FP
authors.lastname: Chávez
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002701:Chloramphenicol / Q000494:pharmacology; D002939:Ciprofloxacin / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D046228:Microarray Analysis / Q000379:methods*; D009154:Mutation; D010641:Phenotype; D017856:Phosphotransferases (Phosphate Group Acceptor) / Q000235:genetics*; D011122:Polyphosphates / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology* / Q000472:pathogenicity; D012293:Rifampin / Q000494:pharmacology; D014774:Virulence / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D011122:Polyphosphates; D002939:Ciprofloxacin; D002701:Chloramphenicol; D017856:Phosphotransferases (Phosphate Group Acceptor); C022424:polyphosphate kinase; D012293:Rifampin