title: High-performance liquid chromatographic method optimization for ondansetron assay in extemporaneous topical gel and in marketed products.
abstract: The compounding and evaluation of ondansetron hydrochloride dihydrate topical gel, 2.5% w/w, were conducted in this study. The gelling agent was Carbopol 940. Ethanol 70% in purified water was used to dissolve the drug and disperse the gelling agent. A gel was formed by adding drops of 0.1 N sodium hydroxide solution. To assay this gel, we developed a simple and reproducible stability--indicating high-performance liquid chromatographic method. This method was validated for specificity, accuracy, and precision. The compounded gel was assayed in triplicate, and the average recovery was 98.3%. Ondansetron marketed products were analyzed for comparison with the compounded formulation. Assay, accuracy, and precision data of the compounded topical gel were comparable to the marketed products.
authors.forename: Masuda
authors.initials: M
authors.lastname: Quamrun
authors.forename: Rashid
authors.initials: R
authors.lastname: Mamoon
authors.forename: Shams
authors.initials: S
authors.lastname: Nasheed
authors.forename: Mullins
authors.initials: M
authors.lastname: Randy
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005782:Gels; D017294:Ondansetron / Q000032:analysis*; D013607:Tablets
chemical_list: D005782:Gels; D013607:Tablets; D017294:Ondansetron