title: Analysis of Lipophilic and Hydrophilic Bioactive Compounds Content in Sea Buckthorn (Hippophaë rhamnoides L.) Berries.
abstract: The aim of this study was to determine selected phytochemicals in berries of eight sea buckthorn (Hippophaë rhamnoides subsp. mongolica) cultivars, including lipophilic and hydrophilic compounds. In the experiment chromatographic analyses, GC (phytosterols and fatty acids), UPLC-PDA-FL, LC-MS (polyphenols), and HPLC (L-ascorbic acid), as well spectrophotometric method (total carotenoids) were used. The lipid fraction isolated from whole fruit contained 14 phytosterols (major compounds β-sitosterol > 24-methylenecykloartanol > squalene) and 11 fatty acids in the order MUFAs > SFAs > PUFAs. Carotenoids occurred in concentrations between 6.19 and 23.91 mg/100 g fresh weight (fw) (p < 0.05). The major polyphenol group identified in berries was flavonols (mean content of 311.55 mg/100 g fw), with the structures of isorhamnetin (six compounds), quercetin (four compounds), and kaempferol (one compound) glycosides. Examined sea buckthorn cultivars were characterized also by a high content of L-ascorbic acid in a range from 52.86 to 130.97 mg/100 g fw (p < 0.05).
authors.affiliation: †Department of Fruit and Vegetable Technology, Wrocław University of Environmental and Life Sciences, Chełmońskiego 37 Street, 51 630 Wrocław, Poland.
authors.forename: Mirosława
authors.initials: M
authors.lastname: Teleszko
authors.affiliation: †Department of Fruit and Vegetable Technology, Wrocław University of Environmental and Life Sciences, Chełmońskiego 37 Street, 51 630 Wrocław, Poland.
authors.forename: Aneta
authors.initials: A
authors.lastname: Wojdyło
authors.affiliation: ‡Institute of Food Technology of Plant Origin, Poznań University of Life Sciences, Wojska Polskiego 31 Street, 60 624 Poznań, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Rudzińska
authors.affiliation: †Department of Fruit and Vegetable Technology, Wrocław University of Environmental and Life Sciences, Chełmońskiego 37 Street, 51 630 Wrocław, Poland.
authors.forename: Jan
authors.initials: J
authors.lastname: Oszmiański
authors.affiliation: §Department of Pomology, Gene Resources and Nurseries, Research Institute of Horticulture, Konstytucji 3 Maja 1/3 Street, 96-100 Skierniewice, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Golis
mesh_terms: D002338:Carotenoids / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005638:Fruit / Q000737:chemistry; D031281:Hippophae / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D013058:Mass Spectrometry; D015394:Molecular Structure; D010840:Phytosterols / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*
chemical_list: D010840:Phytosterols; D010936:Plant Extracts; D002338:Carotenoids
keywords: bioactive compounds; fatty acids; phytosterols; polyphenols; sea buckthorn