title: In-coupled syringe assisted octanol-water partition microextraction coupled with high-performance liquid chromatography for simultaneous determination of neonicotinoid insecticide residues in honey.
abstract: A simple and fast method namely in-coupled syringe assisted octanol-water partition microextraction combined with high performance liquid chromatography (HPLC) has been developed for the extraction, preconcentration and determination of neonicotinoid insecticide residues (e.g. imidacloprid, acetamiprid, clothianidin, thiacloprid, thiamethoxam, dinotefuran, and nitenpyram) in honey. The experimental parameters affected the extraction efficiency, including kind and concentration of salt, kind of disperser solvent and its volume, kind of extraction solvent and its volume, shooting times and extraction time were investigated. The extraction process was carried out by rapid shooting of two syringes. Therefore, rapid dispersion and mass transfer processes was created between phases, and thus affects the extraction efficiency of the proposed method. The optimum extraction conditions were 10.00 mL of aqueous sample, 10% (w/v) Na2SO4, 1-octanol (100µL) as an extraction solvent, shooting 4 times and extraction time 2min. No disperser solvent and centrifugation step was necessary. Linearity was obtained within the range of 0.1-3000 ngmL(-1), with the correlation coefficients greater than 0.99. The high enrichment factor of the target analytes was 100 fold and low limit of detection (0.25-0.50 ngmL(-1)) could be obtained. This proposed method has been successfully applied in the analysis of neonicotinoid residues in honey, and good recoveries in the range of 96.93-107.70% were obtained.
authors.affiliation: Creative Chemistry and Innovation Research Unit, Department of Chemistry and Center for Innovation in Chemistry, Faculty of Science, Mahasarakham University, Mahasarakham 44150, Thailand. Electronic address: jitlada_v@hotmail.com.
authors.forename: Jitlada
authors.initials: J
authors.lastname: Vichapong
authors.affiliation: Materials Chemistry Research Center, Department of Chemistry and Center for Innovation in Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Rodjana
authors.initials: R
authors.lastname: Burakham
authors.affiliation: Materials Chemistry Research Center, Department of Chemistry and Center for Innovation in Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Supalax
authors.initials: S
authors.lastname: Srijaranai
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006146:Guanidines / Q000032:analysis; D006722:Honey / Q000032:analysis*; D007093:Imidazoles / Q000032:analysis; D007306:Insecticides / Q000032:analysis*; D059627:Liquid Phase Microextraction / Q000379:methods*; D000073943:Neonicotinoids; D009574:Nitro Compounds / Q000032:analysis; D000442:Octanols / Q000737:chemistry*; D010078:Oxazines / Q000032:analysis; D011725:Pyridines / Q000032:analysis; D013594:Syringes; D000077922:Thiamethoxam; D013843:Thiazines / Q000032:analysis; D013844:Thiazoles / Q000032:analysis; D014867:Water / Q000737:chemistry*
chemical_list: D006146:Guanidines; D007093:Imidazoles; D007306:Insecticides; D000073943:Neonicotinoids; D009574:Nitro Compounds; D000442:Octanols; D010078:Oxazines; D011725:Pyridines; D013843:Thiazines; D013844:Thiazoles; D014867:Water; C465368:dinotefuran; C082359:imidacloprid; D000077922:Thiamethoxam; C417209:thiacloprid
keywords: Extraction; HPLC; Honey; In-coupled syringe; Neonicotinoid insecticides; Octanol–water partition