title: Fabrication of a totally renewable off-channel amperometric platform for microchip electrophoresis.
abstract: In this approach, a novel method to fabricate an integrated amperometric platform used in off-channel electrophoresis has been introduced. A simple screen printed protocol combining a wet etching procedure was used to define the pattern on a glass substrate, and whole electrodes were constructed by filling the conductive carbon ink into the etched cavities. A simple Teflon tape was used to align this platform with the micro-channel, and the variation of reassembling of this device can be down to 2.2% without the assistance of microscope. This device was characterized by dopamine (DA) and catechol (CA), and the width of half peak is around 4s, even a 100 μm double T shape injection design and a 550 μm working electrode were used in this work. Under the optimum condition, this device possesses a low background with a noise level of 1.4 pA (peak to peak). The linear range for DA and CA are 0.1-100 μM (R = 0.998) and 0.2-200 μM (R = 0.996) with a theoretical plate number of 1.57 × 10(4) and 3.46 × 10(4) (plate/m), respectively.
authors.affiliation: Department of Chemistry, Tamkang University, Tamsui 25137, Taiwan.
authors.forename: Chi-Hao
authors.initials: CH
authors.lastname: Chen
authors.affiliation: Department of Chemistry, Tamkang University, Tamsui 25137, Taiwan.
authors.forename: Ya-Ting
authors.initials: YT
authors.lastname: Lin
authors.affiliation: Department of Chemistry, Tamkang University, Tamsui 25137, Taiwan. Electronic address: mslin@mail.tku.edu.tw.
authors.forename: Meng-Shan
authors.initials: MS
authors.lastname: Lin
mesh_terms: D002244:Carbon / Q000737:chemistry; D002396:Catechols / Q000032:analysis*; D004298:Dopamine / Q000032:analysis*; D004566:Electrodes; D046209:Electrophoresis, Microchip / Q000295:instrumentation; D004867:Equipment Design; D057230:Limit of Detection
chemical_list: D002396:Catechols; D002244:Carbon; C034221:catechol; D004298:Dopamine
keywords: Electrochemical detection; Off-channel; Off-channel microchip; Renewable electrode; Screen printed