title: Electroacupuncture improves microcirculation and neuronal morphology in the spinal cord of a rat model of intervertebral disc extrusion.
abstract: Most studies on spinal cord neuronal injury have focused on spinal cord tissue histology and the expression of nerve cell damage and repair-related genes. The importance of the microcirculation is often ignored in spinal cord injury and repair research. Therefore, in this study, we established a rat model of intervertebral disc extrusion by inserting a silica gel pad into the left ventral surface of T13. Electroacupuncture was used to stimulate the bilateral Zusanli point (ST36) and Neiting point (ST44) for 14 days. Compared with control animals, blood flow in the first lumbar vertebra (L1) was noticeably increased in rats given electroacupuncture. Microvessel density in the T13 segment of the spinal cord was increased significantly as well. The number of normal neurons was higher in the ventral horn of the spinal cord. In addition, vacuolation in the white matter was lessened. No obvious glial cell proliferation was visible. Furthermore, hindlimb motor function was improved significantly. Collectively, our results suggest that electroacupuncture can improve neuronal morphology and microcirculation, and promote the recovery of neurological functions in a rat model of intervertebral disc extrusion.
authors.affiliation: Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing University of Agriculture, Beijing, China.
authors.forename: Dai-Xun
authors.initials: DX
authors.lastname: Jiang
authors.affiliation: Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing University of Agriculture, Beijing, China.
authors.forename: Zhi-Song
authors.initials: ZS
authors.lastname: Lu
authors.affiliation: Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing University of Agriculture, Beijing, China ; Department of Veterinary Biochemistry, Nippon Veterinary and Life Science University, Musashino, Tokyo, Japan.
authors.forename: Ge-Bin
authors.initials: GB
authors.lastname: Li
authors.affiliation: Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing University of Agriculture, Beijing, China.
authors.forename: Sheng-Yong
authors.initials: SY
authors.lastname: Sun
authors.affiliation: Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing University of Agriculture, Beijing, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Mu
authors.affiliation: Department of Veterinary Biochemistry, Nippon Veterinary and Life Science University, Musashino, Tokyo, Japan.
authors.forename: Peter
authors.initials: P
authors.lastname: Lee
authors.affiliation: Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing University of Agriculture, Beijing, China.
authors.forename: Wu
authors.initials: W
authors.lastname: Chen
keywords: NSFC grants; blood circulation; electroacupuncture; inflammation; intervertebral disc; motor function; nerve regeneration; neural regeneration; neurons; neuroprotection