title: Prevalence of nontuberculous mycobacteria among extrapulmonary tuberculosis cases in tertiary care centers in Northern India.
abstract: The reports of nontuberculous mycobacteria (NTM) associated with extrapulmonary diseases are increasing in tertiary care hospitals. Despite a significant increase in knowledge about NTM infections, they still represent a diagnostic and therapeutic challenge. The aim of this study is to know the prevalence of NTN among extrapulmonary tuberculosis cases in tertiary care centers in Northern India. A total of 227 culture positive isolates from 756 cases were tested for niacin production and catalase assay. BIO-LINE SD Ag MPT64 TB test and final identification and differentiation between MTBC and different species of NTM were further confirmed by GenoType Mycobacterium CM/AS assay. 71 cases (9.3%) were positive for AFB by ZN staining and 227 cases (30.1%) were positive for mycobacteria by culture. Niacin production and catalase activity were negative in 62/227 (27.4%) strains and after using a panel of different biochemicals and final confirmation by GenoType Mycobacterium CM assay. Out of 227 cultures tested, 165 (72.6%) strains were confirmed as M. tuberculosis complex, and 62 (27.4%) were confirmed as NTM. The most common NTM species identified were M. fortuitum 17 (27.5%) and M. intracellulare 13 (20.9%). The rapid identification of NTM species may help in targeted therapy and management of the diseases.
authors.affiliation: Department of Microbiology, All India Institute of Medical Sciences, Jodhpur, Rajasthan 342005, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Maurya
authors.affiliation: Department of Microbiology, All India Institute of Medical Sciences, Jodhpur, Rajasthan 342005, India.
authors.forename: V L
authors.initials: VL
authors.lastname: Nag
authors.affiliation: Department of Pulmonary Medicine, King George Medical University, Lucknow, Uttar Pradesh 226003, India.
authors.forename: S
authors.initials: S
authors.lastname: Kant
authors.affiliation: Department of Pulmonary Medicine, King George Medical University, Lucknow, Uttar Pradesh 226003, India.
authors.forename: R A S
authors.initials: RA
authors.lastname: Kushwaha
authors.affiliation: Department of Microbiology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, Uttar Pradesh 226014, India.
authors.forename: M
authors.initials: M
authors.lastname: Kumar
authors.affiliation: Department of Microbiology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, Uttar Pradesh 226014, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Singh
authors.affiliation: Department of Microbiology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, Uttar Pradesh 226014, India.
authors.forename: T N
authors.initials: TN
authors.lastname: Dhole
mesh_terms: D015373:Bacterial Typing Techniques; D005260:Female; D005838:Genotype; D006801:Humans; D007194:India / Q000453:epidemiology; D008297:Male; D009161:Mycobacterium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D015995:Prevalence; D062606:Tertiary Care Centers; D014376:Tuberculosis / Q000453:epidemiology / Q000235:genetics / Q000382:microbiology