title: Chemical Constituents of the Culture Broth of Phellinus linteus and Their Antioxidant Activity.
abstract: The medicinal fungus Phellinus linteus, in the family Hymenochaetaceae, has been used as a traditional medicine for the treatment of various diseases. In this study, the chemical constituents of the culture broth of P. linteus were investigated. P. linteus was cultured in potato dextrose broth medium, and the culture broth was extracted with ethyl acetate. The ethyl acetate-soluble portion was concentrated and subjected to ODS column chromatography, followed by Sephadex LH-20 column chromatography. Six compounds (1~6) were purified by preparative reversed-phase high-performance liquid chromatography. Spectroscopic methods identified their structures as caffeic acid (1), inotilone (2), 4-(3,4-dihydroxyphenyl)-3-buten-2-one (3), phellilane H (4), (2E,4E)-(+)-4'-hydroxy-γ-ionylideneacetic acid (5), and (2E,4E)-γ-ionylideneacetic acid (6). Compounds 1, 2, and 3 exhibited potent dose-dependent antioxidant activity.
authors.affiliation: Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University, Iksan 570-752, Korea.
authors.forename: Myeong-Seok
authors.initials: MS
authors.lastname: Lee
authors.affiliation: Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University, Iksan 570-752, Korea.
authors.forename: Byung Soon
authors.initials: BS
authors.lastname: Hwang
authors.affiliation: Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University, Iksan 570-752, Korea.
authors.forename: In-Kyoung
authors.initials: IK
authors.lastname: Lee
authors.affiliation: Korea National College of Agriculture and Fisheries, Hwaseong 445-760, Korea.
authors.forename: Geon-Sik
authors.initials: GS
authors.lastname: Seo
authors.affiliation: Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University, Iksan 570-752, Korea.
authors.forename: Bong-Sik
authors.initials: BS
authors.lastname: Yun
keywords: Antioxidant; Caffeic acid; Inotilone; Medicinal fungus; Phellinus linteus