title: Relationship of tumor marker CA125 and ovarian tumor stem cells: preliminary identification.
abstract: OBJECTIVE
  The purpose of this study is to identify a prospective association between CA125 and tumorigenic ovarian cancer cells, using the new method of orthotopic transplantation (1).
  
  
  METHODS
  After making the surgical ovarian cancer specimen into cell suspension, we separated the tumorigenic cells from the nontumorigenic cancer cells based on cell surface marker (cancer antigen CA125 and lineage markers) expression. We developed a SCID mice model in which the CA125+/ lineage- and CA125-/ lineage- cells were injected into ovarian parenchyma by use of a microinjector. As a measure of effectiveness of tumor-forming, tumor weight, abdominal distension, ascites volume and activity, subcutaneous fat were determined or observed. Immunohistochemistry was done to determine tumor cell markers.
  
  
  RESULTS
  We found that the cells of CA125+/ lineage- were able to form new tumors; whereas, an equal quantity of CA125-/lineage- cells failed to form any tumors. The new generated tumor contained additional CA125-/lineage- tumorigenic cells as well as the phenotypically diverse population of nontumorigenic cells. Quantities were judged to be significantly different P < 0.0001.
  
  
  CONCLUSIONS
  CA125+/ lineage- cells, which may be ovarian cancer stem cells, were the source for tumor recurrence. The strategies designed to target this cell population may lead to more effective therapies.
authors.affiliation: Affiliated Hospital of HeBei University, Baoding City, HeBei Province, China. 15033217346@163.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: The Third Hospital of Baoding/Baoding Cancer Hospital, Baoding City, HeBei Province, China. 67240184@qq.com.
authors.forename: Yongan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Obstetrics and Gynecology, Third Affiliated Hospital, Guangzhou Medical College, Guangdong Province, China. 3239621085@qq.com.
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Affiliated Hospital of HeBei University, Baoding City, HeBei Province, China. 282361218@qq.com.
authors.forename: Xinping
authors.initials: X
authors.lastname: Gao
authors.affiliation: Affiliated Hospital of HeBei University, Baoding City, HeBei Province, China. rongrenlong@163.com.
authors.forename: Weiming
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Epidemiology and Health Statistic, College of Public Health of Hebe University, Baoding City, HeBei Province, China. 63586988@qq.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Epidemiology and Health Statistic, College of Public Health of Hebe University, Baoding City, HeBei Province, China. 810718847@qq.com.
authors.forename: Haipeng
authors.initials: H
authors.lastname: He
authors.affiliation: Affiliated Hospital of HeBei University, Baoding City, HeBei Province, China. zhanghui98765@163.com.
authors.forename: Yijuan
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Affiliated Hospital of HeBei University, Baoding City, HeBei Province, China. 2804480416@qq.com.
authors.forename: Kun
authors.initials: K
authors.lastname: Pan
authors.affiliation: Affiliated Hospital of HeBei University, Baoding City, HeBei Province, China. hx-zh@sohu.com.
authors.forename: Hongli
authors.initials: H
authors.lastname: Wu
authors.affiliation: Affiliated Hospital of HeBei University, Baoding City, HeBei Province, China. 513983108@qq.com.
authors.forename: Junrong
authors.initials: J
authors.lastname: Shi
authors.affiliation: Affiliated Hospital of HeBei University, Baoding City, HeBei Province, China. 56800304@qq.com.
authors.forename: Huiling
authors.initials: H
authors.lastname: Xue
authors.affiliation: Affiliated Hospital of HeBei University, Baoding City, HeBei Province, China. 13323026959@163.com.
authors.forename: Ling
authors.initials: L
authors.lastname: Liang
authors.affiliation: Affiliated Hospital of HeBei University, Baoding City, HeBei Province, China. 18903363120@163.com.
authors.forename: Zhihuci
authors.initials: Z
authors.lastname: Cai
authors.affiliation: Department of Obstetrics and Gynecology, Qingyuan County Hospital, Baoding City, HeBei Province, China. 75399384@qq.com.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Department of Obstetrics and Gynecology, Wangdu County Hospital, Baoding City, HeBei Province, China. 694894971@qq.com.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis*; D018394:CA-125 Antigen / Q000032:analysis / Q000096:biosynthesis*; D000077216:Carcinoma, Ovarian Epithelial; D004195:Disease Models, Animal; D005260:Female; D005434:Flow Cytometry; D064593:Heterografts; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis / Q000096:biosynthesis*; D051379:Mice; D016513:Mice, SCID; D018613:Microscopy, Confocal; D009375:Neoplasms, Glandular and Epithelial / Q000473:pathology*; D014411:Neoplastic Stem Cells / Q000473:pathology*; D010051:Ovarian Neoplasms / Q000473:pathology*
chemical_list: D014408:Biomarkers, Tumor; D018394:CA-125 Antigen; C451759:MUC16 protein, human; D008565:Membrane Proteins