title: Transcriptome analysis of Sulfolobus solfataricus infected with two related fuselloviruses reveals novel insights into the regulation of CRISPR-Cas system.
abstract: Fuselloviruses SSV1 and SSV2 are model systems to investigate virus-host relationships in stably infected cells thanks to their temperate nature. Although they are very similar in morphology, genome organization and gene synteny, their replication is induced by different stimuli, i.e.: by UV-light exposure (for SSV1) and by the growth progression of the host (for SSV2). In this study, we have analysed global gene expression in SSV1- and SSV2-lysogens of Sulfolobus solfataricus P2 in the absence of any stimuli. Additionally, the interplay among SSV1, SSV2 and the host has been investigated in a double-infected strain to explore both virus-host and virus-virus interactions. Whereas SSV1 did not induce major changes of the host gene expression, SSV2 elicited a strong host response, which includes the transcriptional activation of CRISPR loci and cas genes. As a consequence, a significant decrease of the SSV2 copy number has been observed, which in turn led to provirus-capture into the host chromosome. Results of this study have revealed novel aspects of the host-viral interaction in the frame of the CRISPR-response.
authors.affiliation: Dipartimento di Biologia, Università degli Studi di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cinthia, 80126 Napoli, Italy.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Fusco
authors.affiliation: Dipartimento di Scienze Chimiche, Università degli Studi di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cinthia, 80126 Napoli, Italy.
authors.forename: Rossana
authors.initials: R
authors.lastname: Liguori
authors.affiliation: Dipartimento di Biologia, Università degli Studi di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cinthia, 80126 Napoli, Italy.
authors.forename: Danila
authors.initials: D
authors.lastname: Limauro
authors.affiliation: Dipartimento di Biologia, Università degli Studi di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cinthia, 80126 Napoli, Italy.
authors.forename: Simonetta
authors.initials: S
authors.lastname: Bartolucci
authors.affiliation: Danish Archaea Centre, Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, DK-2200 Copenhagen N, Denmark. Electronic address: qunxin@bio.ku.dk.
authors.forename: Qunxin
authors.initials: Q
authors.lastname: She
authors.affiliation: Dipartimento di Biologia, Università degli Studi di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cinthia, 80126 Napoli, Italy. Electronic address: contursi@unina.it.
authors.forename: Patrizia
authors.initials: P
authors.lastname: Contursi
mesh_terms: D064113:CRISPR-Cas Systems / Q000502:physiology*; D019241:Fuselloviridae; D020869:Gene Expression Profiling; D019848:Gene Expression Regulation, Archaeal / Q000502:physiology; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D048229:Sulfolobus solfataricus / Q000235:genetics* / Q000821:virology*; D059467:Transcriptome; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D014764:Viral Proteins
keywords: CRISPR-Cas system; Fuselloviridae; Sulfolobus solfataricus; Sulfolobus spindle-shaped virus; Transcriptome analysis