title: Subcellular quantitative proteomic analysis reveals host proteins involved in human cytomegalovirus infection.
abstract: Viral replication requires host cell macromolecules and energy, although host cells can alter their protein expression to restrict viral replication. To study the host cell response to human cytomegalovirus (HCMV) infection, a stable isotope labeling by amino acids in cell culture (SILAC)-based subcellular quantitative proteomic study of HCMV-infected human embryo lung fibroblast (HEL) cells was performed, and a total of 247 host proteins were identified as differentially regulated by HCMV. Western blotting and immunofluorescence confocal microscopy were performed to validate the data sets. Gene Ontology analysis indicated that cellular processes involving the metabolism, localization and immune system were regulated as a result of HCMV infection. Functional analysis of selected regulated proteins revealed that knockdown of HNRPD, PHB2 and UB2V2 can increase HCMV replication, while knockdown of A4 and KSRP resulted in decreased HCMV replication. Our study may improve our understanding of the dynamic interactions between HCMV and its host and provide multiple potential targets for anti-HCMV agent research.
authors.affiliation: State Key Laboratory of Virology, Chinese Academy of Sciences, Wuhan, China; Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Chai
authors.affiliation: State Key Laboratory of Virology, Chinese Academy of Sciences, Wuhan, China; College of Life Sciences, Wuhan University, Wuhan, China.
authors.forename: Hao-Yu
authors.initials: HY
authors.lastname: Li
authors.affiliation: State Key Laboratory of Virology, Chinese Academy of Sciences, Wuhan, China; Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Virology, Chinese Academy of Sciences, Wuhan, China; College of Life Sciences, Wuhan University, Wuhan, China.
authors.forename: Xiu-Juan
authors.initials: XJ
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Virology, Chinese Academy of Sciences, Wuhan, China; College of Life Sciences, Wuhan University, Wuhan, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Virology, Chinese Academy of Sciences, Wuhan, China; Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Shaobo
authors.initials: S
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Virology, Chinese Academy of Sciences, Wuhan, China; College of Life Sciences, Wuhan University, Wuhan, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Virology, Chinese Academy of Sciences, Wuhan, China; Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. Electronic address: zhangleike@wh.iov.cn.
authors.forename: Lei-Ke
authors.initials: LK
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Virology, Chinese Academy of Sciences, Wuhan, China; Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. Electronic address: xiaogf@wh.iov.cn.
authors.forename: Gengfu
authors.initials: G
authors.lastname: Xiao
mesh_terms: D002460:Cell Line; D003587:Cytomegalovirus / Q000502:physiology*; D003586:Cytomegalovirus Infections / Q000235:genetics / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism* / Q000821:virology; D000081035:Heterogeneous Nuclear Ribonucleoprotein D0; D034502:Heterogeneous-Nuclear Ribonucleoprotein D / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008025:Ligases / Q000235:genetics / Q000378:metabolism; D000091402:Prohibitins; D040901:Proteomics; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D044763:Ubiquitin-Conjugating Enzymes; D014779:Virus Replication / Q000502:physiology*
chemical_list: C000630986:HNRNPD protein, human; D000081035:Heterogeneous Nuclear Ribonucleoprotein D0; D034502:Heterogeneous-Nuclear Ribonucleoprotein D; C105789:KHSRP protein, human; C000717331:PHB2 protein, human; D000091402:Prohibitins; D016601:RNA-Binding Proteins; D012097:Repressor Proteins; D015534:Trans-Activators; C114715:UBE2V2 protein, human; D044763:Ubiquitin-Conjugating Enzymes; D008025:Ligases
keywords: Human cytomegalovirus; Quantitative proteomics; siRNA