title: Resonance assignment of PsbP: an extrinsic protein from photosystem II of Spinacia oleracea.
abstract: PsbP (23 kDa) is an extrinsic eukaryotic protein of photosystem II found in the thylakoid membrane of higher plants and green algae. It has been proven to be indispensable for proper functioning of the oxygen evolving complex. By interaction with other extrinsic proteins (PsbQ, PsbO and PsbR), it modulates the concentration of two cofactors of the water splitting reaction, Ca(2+) and Cl(-). The crystallographic structure of PsbP from Spinacia oleracea lacks the N-terminal part as well as two inner regions which were modelled as loops. Those unresolved parts are believed to be functionally crucial for the binding of PsbP to the thylakoid membrane. In this NMR study we report (1)H, (15)N and (13)C resonance assignments of the backbone and side chain atoms of the PsbP protein. Based on these data, an estimate of the secondary structure has been made. The structural motifs found fit the resolved parts of the crystallographic structure very well. In addition, the complete assignment set provides preliminary insight into the dynamic regions.
authors.affiliation: Institute of Organic Chemistry, Johannes Kepler University, Altenbergerstraße 69, 4040, Linz, Austria.|Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic.
authors.forename: Adriana
authors.initials: A
authors.lastname: Rathner
authors.affiliation: Institute of Organic Chemistry, Johannes Kepler University, Altenbergerstraße 69, 4040, Linz, Austria.
authors.forename: Kousik
authors.initials: K
authors.lastname: Chandra
authors.affiliation: Institute of Organic Chemistry, Johannes Kepler University, Altenbergerstraße 69, 4040, Linz, Austria.|Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Rathner
authors.affiliation: Institute of Organic Chemistry, Johannes Kepler University, Altenbergerstraße 69, 4040, Linz, Austria.|Lohmann Animal Health, Heinz-Lohmann-Straße 4, 27472, Cuxhaven, Germany.
authors.forename: Michaela
authors.initials: M
authors.lastname: Horničáková
authors.affiliation: Institute of Organic Chemistry, Johannes Kepler University, Altenbergerstraße 69, 4040, Linz, Austria.|Centre de RMN à très Hauts Champs, Institut des Sciences Analytiques, Université de Lyon, 5 Rue de la Doua, 69100, Villeurbanne, France.
authors.forename: Judith
authors.initials: J
authors.lastname: Schlagnitweit
authors.affiliation: Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic.|Centrum of Nanobiology and Structural Biology, Institute of Microbiology, Academy of Sciences of the Czech Republic, Nové Hrady, Czech Republic.
authors.forename: Jaroslava
authors.initials: J
authors.lastname: Kohoutová
authors.affiliation: Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic.|Centrum of Nanobiology and Structural Biology, Institute of Microbiology, Academy of Sciences of the Czech Republic, Nové Hrady, Czech Republic.
authors.forename: Rüdiger
authors.initials: R
authors.lastname: Ettrich
authors.affiliation: Institute of Organic Chemistry, Johannes Kepler University, Altenbergerstraße 69, 4040, Linz, Austria. norbert.mueller@jku.at.|Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic. norbert.mueller@jku.at.
authors.forename: Norbert
authors.initials: N
authors.lastname: Müller
mesh_terms: D000595:Amino Acid Sequence; D018360:Crystallography, X-Ray; D008969:Molecular Sequence Data; D045332:Photosystem II Protein Complex / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D017433:Protein Structure, Secondary; D066244:Proton Magnetic Resonance Spectroscopy; D018724:Spinacia oleracea / Q000737:chemistry*
chemical_list: D045332:Photosystem II Protein Complex; D010940:Plant Proteins
keywords: Dynamic regions; Intrinsic disorder; Oxygen evolving complex; Photosystem II; PsbP