title: Multicolor RGB Marking Allows Morphometric and Functional Analysis of Hippocampal Granule Neurons at the Single-Cell Level.
abstract: Identifying and tracking cells in the brain is a challenging task, requiring advanced molecular biology techniques and precise anatomical information. We have applied the principle of RGB marking to the long-term marking and tracking of progenitor and mature cells in the adult brain. Briefly, multicolor RGB marking is based on the simultaneous, lentiviral vector-mediated expression of three genes encoding fluorescent proteins in the three basic colors (red, green, and blue). Here, we show the application of RGB marking to the stable multicolor marking of adult granule cells in the hippocampus. This technique provides each individual cell with a characteristic hue, which facilitates their anatomical identification and spatial tracking. Multicolor RBG marking of mature neurons can provide an effective approach to dissect the function of individual cells, as it allows combination with functional techniques, facilitating the understanding of complex neuronal populations such as that of the dentate gyrus.
authors.affiliation: 1 Centre for Biological Sciences, University of Southampton , Southampton SO166YD, United Kingdom .
authors.forename: Diego
authors.initials: D
authors.lastname: Gomez-Nicola
authors.affiliation: 2 Research Department Cell and Gene Therapy, Department of Stem Cell Transplantation, University Medical Centre Hamburg-Eppendorf , 20246 Hamburg, Germany .
authors.forename: Kristoffer
authors.initials: K
authors.lastname: Riecken
authors.affiliation: 1 Centre for Biological Sciences, University of Southampton , Southampton SO166YD, United Kingdom .
authors.forename: V Hugh
authors.initials: VH
authors.lastname: Perry
authors.affiliation: 2 Research Department Cell and Gene Therapy, Department of Stem Cell Transplantation, University Medical Centre Hamburg-Eppendorf , 20246 Hamburg, Germany .
authors.forename: Boris
authors.initials: B
authors.lastname: Fehse
mesh_terms: D000818:Animals; D005822:Genetic Vectors / Q000008:administration & dosage / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology*; D016086:Lentivirus / Q000235:genetics; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D057054:Molecular Imaging / Q000379:methods*; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D059010:Single-Cell Analysis / Q000379:methods*
chemical_list: D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins