title: Optimization of amine-rich multilayer thin films for the capture and quantification of prostate-specific antigen.
abstract: It is demonstrated that poly(allylamine hydrochloride)/poly(styrenesulfonate) (PAH/SPS) multilayer films can be successfully tailored for the capture and detection of small biomolecules in dilute concentrations. Based on in vitro results, these films could be potentially applied for rapid and high-throughput diagnosis of dilute biomarkers in serum or tissue. PAH presents functional amino groups that can be further reacted with desired chemistries in order to create customizable and specific surfaces for biomolecule capture. A variety of film assembly characteristics were tested (pH, molecular weight of PAH, and ionic strength) to tune the biotinylation and swelling behavior of these films to maximize detection capabilities. The resultant optimized biotinylated PAH/SPS 9.3/9.3 system was utilized in conjunction with quantum dots (Qdots) to capture and detect a dilute biomarker for prostate cancer, prostate-specific antigen (PSA). Compared to previous work, our system presents a good sensitivity for PSA detection within the clinically relevant range of 0.4-100 ng/mL.
authors.affiliation: †School of Pharmaceutical Sciences, University of Sao Paulo (USP), Sao Paulo, SP, Brazil.
authors.forename: Roberta
authors.initials: R
authors.lastname: Polak
authors.forename: Grinia M
authors.initials: GM
authors.lastname: Bradwell
authors.forename: Jonathan B
authors.initials: JB
authors.lastname: Gilbert
authors.affiliation: §Department of Chemical and Biomolecular Engineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States.
authors.forename: Scott
authors.initials: S
authors.lastname: Danielsen
authors.affiliation: ‡School of Chemical Engineering, University of Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Marisa M
authors.initials: MM
authors.lastname: Beppu
authors.forename: Robert E
authors.initials: RE
authors.lastname: Cohen
authors.forename: Michael F
authors.initials: MF
authors.lastname: Rubner
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis / Q000302:isolation & purification; D006801:Humans; D008297:Male; D015394:Molecular Structure; D010316:Particle Size; D056951:Photoelectron Spectroscopy; D011073:Polyamines / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*; D017430:Prostate-Specific Antigen / Q000032:analysis* / Q000302:isolation & purification*; D011471:Prostatic Neoplasms / Q000737:chemistry*; D045663:Quantum Dots; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; D011073:Polyamines; D011137:Polystyrenes; C063994:polyallylamine; C003321:polystyrene sulfonic acid; D017430:Prostate-Specific Antigen