title: Characterization of MMP-9 gene from a normalized cDNA library of kidney tissue of yellow catfish (Pelteobagrus fulvidraco).
abstract: Matrix metalloproteinase-9 (MMP-9), one of members of the MMP family, is important for the cleaving of structural extracellular matrix (ECM) molecules and involved in inflammatory processes. In this study, MMP-9 cDNA was isolated and characterized from a normalized cDNA library of kidney tissue of yellow catfish (designated as YcMMP-9). The complete sequence of YcMMP-9 cDNA consisted of 2561 nucleotides. The open reading frame potentially encoded a protein of 685 amino acids with a calculated molecular mass of approximately 77.182 kDa. Amino acid sequence of YcMMP-9 have typical characteristics of MMP-9 family and showed highest identity (85.3%) to channel catfish MMP-9. The YcMMP-9 genomic DNA contains 13 exons and 12 introns. Quantitative RT-PCR (qRT-PCR) analysis showed that YcMMP-9 mRNA was constitutively expressed in all examined tissues in normal fish with high expression in head kidney, trunk kidney, blood, and spleen. However, expression of YcMMP-9 mRNA was induced by Aeromonas hydrophila stimulation, especially in these four tissues mentioned above. It indicated that YcMMP-9 was involved in innate immune responses against bacterial infection.
authors.affiliation: College of Life Sciences and Engineering, Henan University of Urban Construction, Pingdingshan 467036, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Ke
authors.affiliation: College of Life Sciences and Engineering, Henan University of Urban Construction, Pingdingshan 467036, China. Electronic address: y-wang401@163.com.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Life Sciences and Engineering, Henan University of Urban Construction, Pingdingshan 467036, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Hong
authors.affiliation: College of Life Sciences and Engineering, Henan University of Urban Construction, Pingdingshan 467036, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Xu
authors.affiliation: College of Life Sciences and Engineering, Henan University of Urban Construction, Pingdingshan 467036, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Chen
authors.affiliation: College of Life Sciences and Engineering, Henan University of Urban Construction, Pingdingshan 467036, China.
authors.forename: Shuai-Bang
authors.initials: SB
authors.lastname: Zhou
mesh_terms: D016980:Aeromonas hydrophila / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D002397:Catfishes / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D015723:Gene Library; D060226:Head Kidney / Q000378:metabolism; D007113:Immunity, Innate; D020780:Matrix Metalloproteinase 9 / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D012333:RNA, Messenger; D020780:Matrix Metalloproteinase 9
keywords: Immune response; MMP-9; Normalized cDNA library; Yellow catfish