title: The influence of organic sample solvents on the separation efficiency of basic compounds under strong cation exchange mode.
abstract: This study investigated the influence of organic sample solvents on separation efficiency of basic compounds under strong cation exchange (SCX) mode. The mixtures of acidic aqueous solution and organic solvent such as acetonitrile, ethanol, methanol and dimethyl sulfoxide (DMSO) were tested as sample solvents. For later-eluting analytes, the increase of sample solvent elution strength was responsible for the decrease of separation efficiency. Thus, sample solvents with weak elution strength could provide high separation efficiencies. For earlier-eluting analytes, the retention of organic sample solvents was the main factor affecting separation efficiency. Weakly retained solvents could provide high separation efficiency. In addition, an optimized approach was proposed to reduce the effect of organic sample solvent, in which low ionic solvent was employed as initial mobile phase in the gradient. At last, the analysis of impurities in hydrophobic drug berberine was performed. The results showed that using acidic aqueous methanol as sample solvents could provide high separation efficiency and good resolution (R>1.5).
authors.affiliation: Key Lab of Separation Science for Analytical Chemistry, Key Lab of Natural Medicine, Liaoning Province, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Long
authors.affiliation: Key Lab of Separation Science for Analytical Chemistry, Key Lab of Natural Medicine, Liaoning Province, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Dongping
authors.initials: D
authors.lastname: Yu
authors.affiliation: Key Lab of Separation Science for Analytical Chemistry, Key Lab of Natural Medicine, Liaoning Province, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Lab of Separation Science for Analytical Chemistry, Key Lab of Natural Medicine, Liaoning Province, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Nana
authors.initials: N
authors.lastname: Du
authors.affiliation: Key Lab of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China.
authors.forename: Yanduo
authors.initials: Y
authors.lastname: Tao
authors.affiliation: Key Lab of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China. Electronic address: meilijuan111@163.com.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Mei
authors.affiliation: Key Lab of Separation Science for Analytical Chemistry, Key Lab of Natural Medicine, Liaoning Province, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China. Electronic address: guozhimou@dicp.ac.cn.
authors.forename: Zhimou
authors.initials: Z
authors.lastname: Guo
authors.affiliation: Key Lab of Separation Science for Analytical Chemistry, Key Lab of Natural Medicine, Liaoning Province, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Xinmiao
authors.initials: X
authors.lastname: Liang
mesh_terms: D000097:Acetonitriles / Q000737:chemistry*; D001599:Berberine / Q000737:chemistry*; D002412:Cations / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004121:Dimethyl Sulfoxide / Q000737:chemistry*; D004340:Drug Contamination; D000431:Ethanol / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D000432:Methanol / Q000737:chemistry*; D015394:Molecular Structure; D012997:Solvents / Q000737:chemistry
chemical_list: D000097:Acetonitriles; D002412:Cations; D012997:Solvents; D001599:Berberine; D000431:Ethanol; D000432:Methanol; D004121:Dimethyl Sulfoxide; C032159:acetonitrile
keywords: Separation efficiency; Solvent elution strength; Solvent injection volume; Strong cation exchange