title: Enhancement of β-Glucosidase Activity from a Brown Rot Fungus Fomitopsis pinicola KCTC 6208 by Medium Optimization.
abstract: β-Glucosidase, which hydrolyzes cellobiose into two glucoses, plays an important role in the process of saccharification of the lignocellulosic biomass. In this study, we optimized the activity of β-glucosidase of brown-rot fungus Fomitopsis pinicola KCTC 6208 using the response surface methodology (RSM) with various concentrations of glucose, yeast extract and ascorbic acid, which are the most significant nutrients for activity of β-glucosidase. The highest activity of β-glucosidase was achieved 3.02% of glucose, 4.35% of yeast extract, and 7.41% ascorbic acid where ascorbic acid was most effective. The maximum activity of β-glucosidase predicted by the RSM was 15.34 U/mg, which was similar to the experimental value 14.90 U/mg at the 16th day of incubation. This optimized activity of β-glucosidase was 23.6 times higher than the preliminary activity value, 0.63 U/mg, and was also much higher than previous values reported in other fungi strains. Therefore, a simplified medium supplemented with a cheap vitamin source, such as ascorbic acid, could be a cost effective mean of increasing β-glucosidase activity.
authors.affiliation: IT Convergence Materials R&D Group, Chungcheong Regional Division, Korea Institute of Industrial Technology (KITECH), Cheonan 330-825, Korea.
authors.forename: Ah Reum
authors.initials: AR
authors.lastname: Park
authors.affiliation: IT Convergence Materials R&D Group, Chungcheong Regional Division, Korea Institute of Industrial Technology (KITECH), Cheonan 330-825, Korea.
authors.forename: Jeong-Hoon
authors.initials: JH
authors.lastname: Park
authors.affiliation: IT Convergence Materials R&D Group, Chungcheong Regional Division, Korea Institute of Industrial Technology (KITECH), Cheonan 330-825, Korea.
authors.forename: Hye-Jin
authors.initials: HJ
authors.lastname: Ahn
authors.affiliation: IT Convergence Materials R&D Group, Chungcheong Regional Division, Korea Institute of Industrial Technology (KITECH), Cheonan 330-825, Korea.
authors.forename: Ji Yeon
authors.initials: JY
authors.lastname: Jang
authors.affiliation: IT Convergence Materials R&D Group, Chungcheong Regional Division, Korea Institute of Industrial Technology (KITECH), Cheonan 330-825, Korea.
authors.forename: Byung Jo
authors.initials: BJ
authors.lastname: Yu
authors.affiliation: Department of Chemical Engineering, Hankyong National University, Anseong 456-749, Korea.
authors.forename: Byung-Hwan
authors.initials: BH
authors.lastname: Um
authors.affiliation: IT Convergence Materials R&D Group, Chungcheong Regional Division, Korea Institute of Industrial Technology (KITECH), Cheonan 330-825, Korea.
authors.forename: Jeong-Jun
authors.initials: JJ
authors.lastname: Yoon
keywords: Ascorbic acid; Fomitopsis pinicola KCTC 6208; Medium optimization; Response surface methodology; β-glucosidase