title: Proteomics of methyl jasmonate induced defense response in maize leaves against Asian corn borer.
abstract: BACKGROUND
  Jasmonic acid (JA) and methyl jasmonate (MeJA) regulate plant development, resistance to stress, and insect attack by inducing specific gene expression. However, little is known about the mechanism of plant defense against herbivore attack at a protein level. Using a high-resolution 2-D gel, we identified 62 MeJA-responsive proteins and measured protein expression level changes.
  
  
  RESULTS
  Among these 62 proteins, 43 proteins levels were increased while 11 proteins were decreased. We also found eight proteins uniquely expressed in response to MeJA treatment. Data are available via ProteomeXchange with identifier PXD001793. The proteins identified in this study have important biological functions including photosynthesis and energy related proteins (38.4%), protein folding, degradation and regulated proteins (15.0%), stress and defense regulated proteins (11.7%), and redox-responsive proteins (8.3%). The expression levels of four important genes were determined by qRT-PCR analysis. The expression levels of these proteins did not correlate well with their translation levels. To test the defense functions of the differentially expressed proteins, expression vectors of four protein coding genes were constructed to express in-fusion proteins in E. coli. The expressed proteins were used to feed Ostrinia furnacalis, the Asian corn borer (ACB). Our results demonstrated that the recombinant proteins of pathogenesis-related protein 1 (PR1) and thioredoxin M-type, chloroplastic precursor (TRXM) showed the significant inhibition on the development of larvae and pupae.
  
  
  CONCLUSIONS
  We found MeJA could not only induce plant defense mechanisms to insects, it also enhanced toxic protein production that potentially can be used for bio-control of ACB.
authors.affiliation: Key Laboratory of Molecular Biology of Heilongjiang Province, College of Life Sciences, Heilongjiang University, Harbin, 150080, China. zhangytong_2006@163.com.|Majorbio Pharm Technology Co., Ltd., Shanghai, 201203, China. zhangytong_2006@163.com.
authors.forename: Yi Tong
authors.initials: YT
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, 571101, China. zhangyuliang@itbb.org.cn.
authors.forename: Yu Liang
authors.initials: YL
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Molecular Biology of Heilongjiang Province, College of Life Sciences, Heilongjiang University, Harbin, 150080, China. schen@ufl.edu.|Department of Biology, Genetics Institute, Plant Molecular and Cellular Biology Program, Interdisciplinary Center for Biotechnology Research, University of Florida, Gainesville, Florida, 32610, USA. schen@ufl.edu.
authors.forename: Si Xue
authors.initials: SX
authors.lastname: Chen
authors.affiliation: Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, 571101, China. guohuayin1997@gmail.com.|Department of Plant Biology and Pathology, Rutgers, The State University of New Jersey, New Brunswick, NJ, 08901, USA. guohuayin1997@gmail.com.
authors.forename: Guo Hua
authors.initials: GH
authors.lastname: Yin
authors.affiliation: Key Laboratory of Molecular Biology of Heilongjiang Province, College of Life Sciences, Heilongjiang University, Harbin, 150080, China. yyll88523@126.com.|Institute of Pesticide Science, Hunan Agricultural University, Changsha, China. yyll88523@126.com.
authors.forename: Ze Zhong
authors.initials: ZZ
authors.lastname: Yang
authors.affiliation: Department of Plant Biology and Pathology, Rutgers, The State University of New Jersey, New Brunswick, NJ, 08901, USA. samantha.leexiii@gmail.com.
authors.forename: Samantha
authors.initials: S
authors.lastname: Lee
authors.affiliation: Key Laboratory of Molecular Biology of Heilongjiang Province, College of Life Sciences, Heilongjiang University, Harbin, 150080, China. liu_chun_guang@yahoo.com.cn.|Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin, 150500, China. liu_chun_guang@yahoo.com.cn.
authors.forename: Chun Guang
authors.initials: CG
authors.lastname: Liu
authors.affiliation: Key Laboratory of Molecular Biology of Heilongjiang Province, College of Life Sciences, Heilongjiang University, Harbin, 150080, China. zhao_dan_dan@yahoo.com.cn.|Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin, 150500, China. zhao_dan_dan@yahoo.com.cn.
authors.forename: Dan Dan
authors.initials: DD
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Molecular Biology of Heilongjiang Province, College of Life Sciences, Heilongjiang University, Harbin, 150080, China. mayukun66@126.com.|Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin, 150500, China. mayukun66@126.com.
authors.forename: Yu Kun
authors.initials: YK
authors.lastname: Ma
authors.affiliation: Key Laboratory of Molecular Biology of Heilongjiang Province, College of Life Sciences, Heilongjiang University, Harbin, 150080, China. 0431sfq@163.com.|Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin, 150500, China. 0431sfq@163.com.
authors.forename: Fu Qiang
authors.initials: FQ
authors.lastname: Song
authors.affiliation: Department of Plant Biology and Pathology, Rutgers, The State University of New Jersey, New Brunswick, NJ, 08901, USA. profmycogirl@yahoo.com.
authors.forename: Joan W
authors.initials: JW
authors.lastname: Bennett
authors.affiliation: Key Laboratory of Molecular Biology of Heilongjiang Province, College of Life Sciences, Heilongjiang University, Harbin, 150080, China. yangfengshan@hlju.edu.cn.|Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin, 150500, China. yangfengshan@hlju.edu.cn.
authors.forename: Feng Shan
authors.initials: FS
authors.lastname: Yang
mesh_terms: D000085:Acetates / Q000378:metabolism*; D000818:Animals; D001208:Asia; D003517:Cyclopentanes / Q000378:metabolism*; D060434:Herbivory; D007915:Lepidoptera / Q000502:physiology*; D054883:Oxylipins / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000378:metabolism*; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D040901:Proteomics; D003313:Zea mays / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D011506:Proteins; C072239:methyl jasmonate