title: Effect of liposomes on rheological and syringeability properties of hyaluronic acid hydrogels intended for local injection of drugs.
abstract: The aim of this work was to thoroughly study the effect of liposomes on the rheological and the syringeability properties of hyaluronic acid (HA) hydrogels intended for the local administration of drugs by injection. Whatever the characteristics of the liposomes added (neutral, positively or negatively charged, with a corona of polyethylene glycol chains, size), the viscosity and the elasticity of HA gels increased in a lipid concentration-dependent manner. Indeed, liposomes strengthened the network formed by HA chains due to their interactions with this polymer. The nature and the resulting effects of these interactions depended on liposome composition and concentration. The highest viscosity and elasticity were observed with liposomes covered by polyethylene glycol chains while neutral liposomes displayed the lowest effect. Despite their high viscosity at rest, all the formulations remained easily injectable through needles commonly used for local injections thanks to the shear-thinning behavior of HA gels. The present study demonstrates that rheological and syringeability tests are both necessary to elucidate the behavior of such systems during and post injection. In conclusion, HA liposomal gels appear to be a promising and versatile formulation platform for a wide range of applications in local drug delivery when an injection is required.
authors.affiliation: Université Paris-Sud, Faculté de pharmacie, Institut Galien Paris-Sud, CNRS UMR 8612, 5 Rue J-B Clément, 92290 Châtenay-Malabry, France.
authors.forename: Naila
authors.initials: N
authors.lastname: El Kechai
authors.affiliation: Université Paris-Sud, Faculté de pharmacie, Institut Galien Paris-Sud, CNRS UMR 8612, 5 Rue J-B Clément, 92290 Châtenay-Malabry, France.
authors.forename: Amélie
authors.initials: A
authors.lastname: Bochot
authors.affiliation: Université Paris-Sud, Faculté de pharmacie, Institut Galien Paris-Sud, CNRS UMR 8612, 5 Rue J-B Clément, 92290 Châtenay-Malabry, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Huang
authors.affiliation: UMR-S 1159 Inserm "Minimally Invasive Robot-based Hearing Rehabilitation", Université Paris VI Pierre et Marie Curie, 46 rue Henri Huchard, 75018 Paris, France.
authors.forename: Yann
authors.initials: Y
authors.lastname: Nguyen
authors.affiliation: UMR-S 1159 Inserm "Minimally Invasive Robot-based Hearing Rehabilitation", Université Paris VI Pierre et Marie Curie, 46 rue Henri Huchard, 75018 Paris, France.
authors.forename: Evelyne
authors.initials: E
authors.lastname: Ferrary
authors.affiliation: Université Paris-Sud, Faculté de pharmacie, Institut Galien Paris-Sud, CNRS UMR 8612, 5 Rue J-B Clément, 92290 Châtenay-Malabry, France. Electronic address: florence.agnely@u-psud.fr.
authors.forename: Florence
authors.initials: F
authors.lastname: Agnely
mesh_terms: D002626:Chemistry, Pharmaceutical; D016503:Drug Delivery Systems; D004548:Elasticity; D005079:Excipients; D005614:Freeze Fracturing; D006820:Hyaluronic Acid / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D007267:Injections; D008081:Liposomes / Q000737:chemistry*; D020442:Poloxamer; D011092:Polyethylene Glycols / Q000737:chemistry; D012212:Rheology; D013594:Syringes; D014783:Viscosity
chemical_list: D005079:Excipients; D020100:Hydrogels; D008081:Liposomes; D020442:Poloxamer; D011092:Polyethylene Glycols; D006820:Hyaluronic Acid
keywords: 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy-poly-(ethyleneglycol)-2000] (PubChem CID: 406952); 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy-poly-(ethyleneglycol)-5000] (PubChem CID: not available); Cholesterol (PubChem CID: 5997); Hyaluronic acid; Hydrogel; Liposomes; Local injection; Phosphatidylcholine (PubChem CID: 52922911); Poloxamer 407 (PubChem CID: 24751); Polyethyleneglycols (PubChem CID: 5327147); Rheology; Sodium hyaluronate (PubChem CID: 3084049); Stearylamine (PubChem CID: 15793); Syringeability; l-α-Phosphatidylglycerol (PubChem CID: 24779550)