title: Severe acute respiratory syndrome coronavirus protein 6 mediates ubiquitin-dependent proteosomal degradation of N-Myc (and STAT) interactor.
abstract: Severe acute respiratory syndrome coronavirus (SARS-CoV) encodes eight accessory proteins, the functions of which are not yet fully understood. SARS-CoV protein 6 (P6) is one of the previously studied accessory proteins that have been documented to enhance viral replication and suppress host interferon (IFN) signaling pathways. Through yeast two-hybrid screening, we identified eight potential cellular P6-interacting proteins from a human spleen cDNA library. For further investigation, we targeted the IFN signaling pathway-mediating protein, N-Myc (and STAT) interactor (Nmi). Its interaction with P6 was confirmed within cells. The results showed that P6 can promote the ubiquitin-dependent proteosomal degradation of Nmi. This study revealed a new mechanism of SARS-CoV P6 in limiting the IFN signaling to promote SARS-CoV survival in host cells.
authors.affiliation: School of Basic Medical Sciences, Wuhan University, Wuhan, 430072, China.
authors.forename: Weijia
authors.initials: W
authors.lastname: Cheng
authors.forename: Shiyou
authors.initials: S
authors.lastname: Chen
authors.forename: Ruiling
authors.initials: R
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Deyin
authors.initials: D
authors.lastname: Guo
mesh_terms: D015723:Gene Library; D054884:Host-Pathogen Interactions; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D059748:Proteolysis; D045473:Severe acute respiratory syndrome-related coronavirus / Q000502:physiology*; D020798:Two-Hybrid System Techniques; D025801:Ubiquitin / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; C099927:NMI protein, human; D025801:Ubiquitin; D014764:Viral Proteins; C507223:protein 6, SARS virus