title: Heterotrophic nitrogen removal by Acinetobacter sp. Y1 isolated from coke plant wastewater.
abstract: A strain of Acinetobacter sp. Y1, which exhibited an amazing ability to remove ammonium, nitrite and nitrate, was isolated from the activated sludge of a coking wastewater treatment plant. The aim of this work was to study the ability, influence factors and possible pathway of nitrogen removal by Acinetobacter sp. Y1. Results showed that maximum removal rate of NH4(+)-N by the strain was 10.28 mg-N/L/h. Carbon source had significant influence on the growth and ammonium removal efficiencies of strain Y1. Pyruvate, citrate and acetate were favourable carbon sources for the strain. Temperature, pH value and shaking speed could affect the growth and nitrogen removal ability. Nitrate or nitrite could be used as a sole nitrogen source for the growth and removed efficiently by the strain. N2 levels increased to 53.74%, 50.21% and 55.13% within 36 h when 100 mg/L NH4(+)-N, NO2(-)-N or NO3(-) -N was used as sole nitrogen source in the gas detection experiment. The activities of hydroxylamine oxidoreductase (HAO), nitrate reductase (NR) and nitrite reductase (NiR), which are key enzymes in heterotrophic nitrification and aerobic denitrification, were all detectable in the strain. Consequently, a possible pathway for ammonium removal by the strain was also suggested.
authors.affiliation: College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, China. Electronic address: yuxiangliu2002@126.com.
authors.forename: YuXiang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Hu
authors.affiliation: Shanxi Meteorological Science Institute, Taiyuan 030002, China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Song
authors.affiliation: College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
authors.forename: Hongping
authors.initials: H
authors.lastname: Chen
authors.affiliation: Shanxi Key Lab of Coal Science and Technology, Taiyuan University of Technology, Taiyuan 030024, China.
authors.forename: YongKang
authors.initials: Y
authors.lastname: Lv
mesh_terms: D000150:Acinetobacter / Q000378:metabolism*; D000332:Aerobiosis; D064751:Ammonium Compounds / Q000302:isolation & purification / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D003077:Coke; D058440:Denitrification; D052836:Heterotrophic Processes; D050901:Nitrate Reductase / Q000378:metabolism; D009566:Nitrates / Q000302:isolation & purification / Q000378:metabolism; D058465:Nitrification; D009572:Nitrite Reductases / Q000378:metabolism; D009573:Nitrites / Q000302:isolation & purification / Q000378:metabolism; D009584:Nitrogen / Q000302:isolation & purification / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism; D012722:Sewage / Q000737:chemistry; D062065:Wastewater / Q000737:chemistry*
chemical_list: D064751:Ammonium Compounds; D002244:Carbon; D003077:Coke; D050901:Nitrate Reductase; D009566:Nitrates; D009572:Nitrite Reductases; D009573:Nitrites; D009584:Nitrogen; D010088:Oxidoreductases; D012722:Sewage; D062065:Wastewater; C021401:hydroxylamine dehydrogenase
keywords: Acinetobacter; Aerobic denitrification; Coke plant wastewater; Heterotrophic nitrification; Nitrogen removal