title: Isolation of a sesquiterpene synthase expressing in specialized epithelial cells surrounding the secretory cavities in rough lemon (Citrus jambhiri).
abstract: Volatile terpenoids such as monoterpenes and sesquiterpenes play multiple roles in plant responses and are synthesized by terpene synthases (TPSs). We have previously isolated a partial TPS gene, RlemTPS4, that responds to microbial attack in rough lemon. In this study, we isolated a full length RlemTPS4 cDNA from rough lemon. RlemTPS4 localized in the cytosol. The recombinant RlemTPS4 protein was obtained using a prokaryotic expression system and GC-MS analysis of the terpenes produced by the RlemTPS4 enzymatic reaction determined that RlemTPS4 produces some sesquiterpenes such as δ-elemene. The RlemTPS4 gene was specifically expressed in specialized epithelial cells surrounding the oil secretory cavities in rough lemon leaf tissue.
authors.affiliation: Faculty of Agriculture and Gene Research Center, Kagawa University, Miki, Kagawa 761-0795, Japan.
authors.forename: Yuya
authors.initials: Y
authors.lastname: Uji
authors.affiliation: Center for Ecological Research, Kyoto University, Otsu, Shiga 520-2113, Japan.
authors.forename: Rika
authors.initials: R
authors.lastname: Ozawa
authors.affiliation: Faculty of Agriculture and Gene Research Center, Kagawa University, Miki, Kagawa 761-0795, Japan.
authors.forename: Hodaka
authors.initials: H
authors.lastname: Shishido
authors.affiliation: Faculty of Agriculture and Gene Research Center, Kagawa University, Miki, Kagawa 761-0795, Japan.
authors.forename: Shiduku
authors.initials: S
authors.lastname: Taniguchi
authors.affiliation: Center for Ecological Research, Kyoto University, Otsu, Shiga 520-2113, Japan.
authors.forename: Junji
authors.initials: J
authors.lastname: Takabayashi
authors.affiliation: Faculty of Agriculture and Gene Research Center, Kagawa University, Miki, Kagawa 761-0795, Japan.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Akimitsu
authors.affiliation: Faculty of Agriculture and Gene Research Center, Kagawa University, Miki, Kagawa 761-0795, Japan. Electronic address: gomiken@ag.kagawa-u.ac.jp.
authors.forename: Kenji
authors.initials: K
authors.lastname: Gomi
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D000595:Amino Acid Sequence; D002957:Citrus / Q000201:enzymology* / Q000235:genetics; D004847:Epithelial Cells / Q000201:enzymology*; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020539:Sequence Analysis, Protein
chemical_list: D010940:Plant Proteins; D012333:RNA, Messenger; D019883:Alkyl and Aryl Transferases; C108877:terpene synthase
keywords: Citrus; Essential oil; Sesquiterpene; Terpene synthase; δ-Elemene