title: Lignan Glycosides from the Twigs of Chaenomeles sinensis and Their Biological Activities.
abstract: Phytochemical investigation of the twigs of Chaenomeles sinensis led to the isolation and identification of six new lignan glycosides, chaenomiside A-F (1-6), along with five known ones (7-11). Their chemical structures were determined by spectroscopic methods, including NMR, MS, ECD, and GC/MS analyses. All the isolated compounds (1-11) were tested for their inhibitory effects on nitric oxide (NO) production in lipopolysaccharide-activated murine microglial cells and the secretion of nerve growth factor (NGF) in a C6 rat glioma cell line. Compound 6 significantly reduced NO levels in the murine microglia BV2 cells with an IC50 value of 21.3 μM, and compounds 1, 3, and 6 were potent stimulants of NGF release with stimulation levels of 151.74 ± 6.77%, 144.31 ± 7.49%, and 167.61 ± 18.5%, respectively.
authors.affiliation: †Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
authors.forename: Chung Sub
authors.initials: CS
authors.lastname: Kim
authors.forename: Lalita
authors.initials: L
authors.lastname: Subedi
authors.forename: Sun Yeou
authors.initials: SY
authors.lastname: Kim
authors.affiliation: ⊥Korea Research Institute of Chemical Technology, Daejeon 305-343, Republic of Korea.
authors.forename: Sang Un
authors.initials: SU
authors.lastname: Choi
authors.affiliation: †Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
authors.forename: Ki Hyun
authors.initials: KH
authors.lastname: Kim
authors.affiliation: †Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
authors.forename: Kang Ro
authors.initials: KR
authors.lastname: Lee
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D017628:Microglia / Q000187:drug effects; D015394:Molecular Structure; D020932:Nerve Growth Factor / Q000378:metabolism; D009569:Nitric Oxide / Q000032:analysis / Q000096:biosynthesis; D018547:Plant Stems / Q000737:chemistry; D051381:Rats; D056910:Republic of Korea; D027824:Rosaceae / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D006027:Glycosides; D017705:Lignans; D008070:Lipopolysaccharides; D009569:Nitric Oxide; D020932:Nerve Growth Factor