title: Comparison of the genexpert enterovirus assay (GXEA) with real-time one step RT-PCR for the detection of enteroviral RNA in the cerebrospinal fluid of patients with meningitis.
abstract: BACKGROUND
  Enteroviruses (EVs) are the leading cause of aseptic meningitis worldwide. Detection of enteroviral RNA in clinical specimens has been demonstrated to improve the management of patient care, especially that of neonates and young children.
  
  
  RESULTS
  To establish a sensitive and reliable assay for routine laboratory diagnosis, we compared the sensitivity and specificity of the GeneXpert Enterovirus Assay (GXEA) with that of the reverse transcription polymerase chain reaction (RT-PCR) based assay referred to as real-time one step RT-PCR (RTo-PCR). The sensitivity/specificity produced by GXEA and RTo-PCR were 100%/100% and 65%/100%, respectively.
  
  
  CONCLUSIONS
  Both methods evaluated in this article can be used for detection of enterovirus in clinical specimens and these nucleic acid amplification methods are useful assays for the diagnosis of enteroviral infection.
authors.affiliation: Division of Vaccine Research, Center for Infectious Diseases, National Institute of Health, Korea Center for Disease Control and Prevention, Cheongju, Republic of Korea. eca_dee@hanmail.net.|Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea. eca_dee@hanmail.net.
authors.forename: JiYoung
authors.initials: J
authors.lastname: Hong
authors.affiliation: Division of Vaccine Research, Center for Infectious Diseases, National Institute of Health, Korea Center for Disease Control and Prevention, Cheongju, Republic of Korea. youn0191@hanmail.net.
authors.forename: Ahyoun
authors.initials: A
authors.lastname: Kim
authors.affiliation: Division of Vaccine Research, Center for Infectious Diseases, National Institute of Health, Korea Center for Disease Control and Prevention, Cheongju, Republic of Korea. 0117772624@daum.net.
authors.forename: Seoyeon
authors.initials: S
authors.lastname: Hwang
authors.affiliation: Division of Vaccine Research, Center for Infectious Diseases, National Institute of Health, Korea Center for Disease Control and Prevention, Cheongju, Republic of Korea. cheonds@daum.net.
authors.forename: Doo-Sung
authors.initials: DS
authors.lastname: Cheon
authors.affiliation: Department of Pediatrics, Catholic University College of Medicine, Suwon, Republic of Korea. jh00mn@catholic.ac.kr.
authors.forename: Jong-Hyen
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Division of Vaccine Research, Center for Infectious Diseases, National Institute of Health, Korea Center for Disease Control and Prevention, Cheongju, Republic of Korea. junewoo1213@korea.kr.
authors.forename: June-Woo
authors.initials: JW
authors.lastname: Lee
authors.affiliation: Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea. pjhak@snu.ac.kr.
authors.forename: Jae-Hak
authors.initials: JH
authors.lastname: Park
authors.affiliation: Division of Vaccine Research, Center for Infectious Diseases, National Institute of Health, Korea Center for Disease Control and Prevention, Cheongju, Republic of Korea. bh4400@daum.net.
authors.forename: Byunghak
authors.initials: B
authors.lastname: Kang
mesh_terms: D004770:Enterovirus / Q000235:genetics / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000134:cerebrospinal fluid / Q000175:diagnosis / Q000821:virology*; D006801:Humans; D008581:Meningitis / Q000134:cerebrospinal fluid / Q000175:diagnosis / Q000821:virology*; D025202:Molecular Diagnostic Techniques; D012367:RNA, Viral / Q000134:cerebrospinal fluid / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
chemical_list: D012367:RNA, Viral