title: Genetic polymorphisms of NAMPT related with susceptibility to esophageal squamous cell carcinoma.
abstract: BACKGROUND
  Nicotinamide phosphoribosyl transferase (Nampt) plays a crucial role in tumorigenesis. The present study examines whether genetic polymorphisms of NAMPT are related to the risk of developing esophageal squamous cell carcinoma (ESCC).
  
  
  METHODS
  A total of 810 subjects were enrolled in this study, including 405 ESCC patients and 405 healthy controls. Using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), genotypes at rs61330082, rs2505568 and rs9034 of NAMPT were identified. Haplotypes were constructed using PHASE software. Multivariate logistic regression models were used to evaluate the potentiating effects of the genotypes, alleles and haplotypes on the development of ESCC.
  
  
  RESULTS
  The presence of genotypes CT and TT and allele T at rs61330082 was less frequent in ESCC cases than in controls (48.89% vs. 53.33%, P < 0.01, 95% CI: 0.33-0.68; 18.52% vs. 30.37%, P < 0.01, 95% CI: 0.22-0.50; 42.96% vs. 57.04%, P < 0.01, 95% CI: 0.38-0.61; respectively). No statistically significant differences existed in the distributions of genotypes or alleles at rs2505568 or rs9034 between ESCC cases and controls. Of five haplotypes constructed, haplotypes CTC, CTT and CAC were higher in ESCC cases (P < 0.01, OR = 1.57, 95% CI: 1.16-2.12; P = 0.04, OR = 1.72, 95% CI: 1.03-2.85; P < 0.01, OR = 3.39, 95% CI: 1.99-5.75; respectively) than in controls.
  
  
  CONCLUSIONS
  Genetic polymorphisms of NAMPT, specifically genotype CC and allele C at rs61330082 as well as haplotypes CTC, CTT and CAC, were significantly correlated with ESCC susceptibility.
authors.affiliation: Digestive Department, Shandong Provincial Qianfoshan Hospital, Shandong University, Jingshi Road 16766#, Jinan, 250014, China. fax8446@126.com.
authors.forename: Chuanzhen
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Infectious Diseases Hospital, Laiwu Hospital, Taishan Medical College, Laiwu, China. daojie120@163.com.
authors.forename: Daojie
authors.initials: D
authors.lastname: Yan
authors.affiliation: Digestive Department, Shandong Provincial Qianfoshan Hospital, Shandong University, Jingshi Road 16766#, Jinan, 250014, China. 951804008@qq.com.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Wang
authors.affiliation: Digestive Department, Shandong Provincial Qianfoshan Hospital, Shandong University, Jingshi Road 16766#, Jinan, 250014, China. xcqys@126.com.
authors.forename: Changqing
authors.initials: C
authors.lastname: Xu
authors.affiliation: Digestive Department, Shandong Provincial Qianfoshan Hospital, Shandong University, Jingshi Road 16766#, Jinan, 250014, China. crbdwj@126.com.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Du
authors.affiliation: Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Laboratory of Genetics, Peking University School of Oncology, Beijing Cancer Hospital & Institute, Beijing, China. ningtao100@126.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Ning
authors.affiliation: Digestive Department, Shandong Provincial Qianfoshan Hospital, Shandong University, Jingshi Road 16766#, Jinan, 250014, China. liuchanghong@sdhospital.com.cn.
authors.forename: Changhong
authors.initials: C
authors.lastname: Liu
authors.affiliation: Digestive Department, Shandong Provincial Qianfoshan Hospital, Shandong University, Jingshi Road 16766#, Jinan, 250014, China. zhangmeijuan@sdhospital.com.cn.
authors.forename: Meijuan
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Digestive Department, Shandong Provincial Qianfoshan Hospital, Shandong University, Jingshi Road 16766#, Jinan, 250014, China. houruiping@sdhospital.com.cn.
authors.forename: Ruiping
authors.initials: R
authors.lastname: Hou
authors.affiliation: Digestive Department, Shandong Provincial Qianfoshan Hospital, Shandong University, Jingshi Road 16766#, Jinan, 250014, China. chenziping1966@163.com.
authors.forename: Ziping
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000368:Aged; D000483:Alleles; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D016022:Case-Control Studies; D016207:Cytokines / Q000235:genetics*; D004938:Esophageal Neoplasms / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D008297:Male; D008875:Middle Aged; D054409:Nicotinamide Phosphoribosyltransferase / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide
chemical_list: D016207:Cytokines; D054409:Nicotinamide Phosphoribosyltransferase; C085455:nicotinamide phosphoribosyltransferase, human