title: Global Transcriptome Analysis Reveals Acclimation-Primed Processes Involved in the Acquisition of Desiccation Tolerance in Boea hygrometrica.
abstract: Boea hygrometrica resurrection plants require a period of acclimation by slow soil-drying in order to survive a subsequent period of rapid desiccation. The molecular basis of this observation was investigated by comparing gene expression profiles under different degrees of water deprivation. Transcripts were clustered according to the expression profiles in plants that were air-dried (rapid desiccation), soil-dried (gradual desiccation), rehydrated (acclimated) and air-dried after acclimation. Although phenotypically indistinguishable, it was shown by principal component analysis that the gene expression profiles in rehydrated, acclimated plants resemble those of desiccated plants more closely than those of hydrated acclimated plants. Enrichment analysis based on gene ontology was performed to deconvolute the processes that accompanied desiccation tolerance. Transcripts associated with autophagy and α-tocopherol accumulation were found to be activated in both air-dried, acclimated plants and soil-dried non-acclimated plants. Furthermore, transcripts associated with biosynthesis of ascorbic acid, cell wall catabolism, chaperone-assisted protein folding, respiration and macromolecule catabolism were activated and maintained during soil-drying and rehydration. Based on these findings, we hypothesize that activation of these processes leads to the establishment of an optimal physiological and cellular state that enables tolerance during rapid air-drying. Our study provides a novel insight into the transcriptional regulation of critical priming responses to enable survival following rapid dehydration in B. hygrometrica.
authors.affiliation: Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China These authors contributed equally to this work.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China These authors contributed equally to this work.
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.affiliation: IMBIO (Molekulare Physiologie und Biotechnologie der Pflanzen), University of Bonn, Kirschallee 1, D-53115 Bonn, Germany Present address: Monsanto Company, 800 North Lindbergh Blvd, St. Louis, MO 63167, USA.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Phillips
authors.affiliation: Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Zhen-Nan
authors.initials: ZN
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Du
authors.affiliation: Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
authors.affiliation: Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Lian-Cheng
authors.initials: LC
authors.lastname: Huang
authors.affiliation: Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Xiao-Fei
authors.initials: XF
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Guang-Hui
authors.initials: GH
authors.lastname: Xu
authors.affiliation: Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Wen-Long
authors.initials: WL
authors.lastname: Li
authors.affiliation: Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Shanghai OE Biomedical Technology Co., Ltd., Shanghai 201210, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Yong-Xiu
authors.initials: YX
authors.lastname: Liu
authors.affiliation: Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China deng@ibcas.ac.cn.
authors.forename: Xin
authors.initials: X
authors.lastname: Deng
mesh_terms: D000064:Acclimatization / Q000235:genetics*; D002473:Cell Wall / Q000235:genetics / Q000378:metabolism; D003890:Desiccation; D020869:Gene Expression Profiling / Q000379:methods*; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D008031:Lignin / Q000378:metabolism; D019684:Magnoliopsida / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014867:Water / Q000378:metabolism; D014868:Water Deprivation
chemical_list: D010940:Plant Proteins; D014867:Water; D008031:Lignin
keywords: Acclimation; Autophagy; Boea hygrometrica; Desiccation tolerance; Gene expression; α-Tocopherol