title: The studies on the toxicity mechanism of environmentally hazardous natural (IAA) and synthetic (NAA) auxin--The experiments on model Arabidopsis thaliana and rat liver plasma membranes.
abstract: This paper concerns the studies towards membrane-damage effect of two auxins: indole-3-acetic acid - IAA and 1-naphthaleneacetic acid - NAA on plant (Arabidopsis thaliana) and animal (rat liver) model membranes. The foregoing auxins are plant growth regulators widely used in agriculture to control the quality of the crop. However, their accumulation in the environment makes them hazardous for the living organisms. The aim of our investigations was to compare the effect of natural (IAA) vs. synthetic (NAA) auxin on the organization of plant and animal model membranes and find a possible correlation between membrane-disturbing effect of these compounds and their toxicity. The collected data evidenced that auxins cause destabilization of membranes, decrease their condensation and weakens interactions of molecules. The alterations in the morphology of model systems were also noticed. The foregoing effects of auxins are concentration-dependent and additionally NAA was found to act on animal vs. plant membranes more selectively than IAA. Interestingly, both IAA and NAA induce the strongest disordering in model lipid system at the concentration, which is frequently reported as toxic to animal and plants. Based on the above findings it was proposed that membrane-damage effect induced by IAA and NAA may be important from the point of view of the mechanism of toxicity of these compounds and cannot be ignored in further investigations in this area.
authors.affiliation: Department of Environmental Chemistry, Faculty of Chemistry, Jagiellonian University, Gronostajowa 3, 30-387 Kraków, Poland. Electronic address: hac@chemia.uj.edu.pl.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Hąc-Wydro
authors.affiliation: Department of Environmental Chemistry, Faculty of Chemistry, Jagiellonian University, Gronostajowa 3, 30-387 Kraków, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Flasiński
mesh_terms: D000818:Animals; D017360:Arabidopsis / Q000737:chemistry / Q000378:metabolism; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000378:metabolism / Q000633:toxicity*; D007210:Indoleacetic Acids / Q000378:metabolism / Q000633:toxicity*; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D008563:Membrane Lipids / Q000737:chemistry / Q000378:metabolism; D008853:Microscopy / Q000379:methods; D009280:Naphthaleneacetic Acids / Q000378:metabolism / Q000633:toxicity*; D010937:Plant Growth Regulators / Q000378:metabolism / Q000633:toxicity; D051381:Rats
chemical_list: D004785:Environmental Pollutants; D007210:Indoleacetic Acids; D008563:Membrane Lipids; D009280:Naphthaleneacetic Acids; D010937:Plant Growth Regulators; C034182:1-naphthaleneacetic acid; C030737:indoleacetic acid
keywords: Auxins; Environmental toxicity; Model membrane; Plant growth regulators