title: [Sedum sarmentosun bunge extraction ameliorated severe acute pancreatitis-induced lung injury: an experimental research]].
abstract: OBJECTIVE
  To explore the effect of Sedum sarmentosum Bunge Extract (SSBE) on severe acute pancreatitis (SAP) induced acute lung injury (ALI) model rats and their excessive inflammatory reactions.
  
  
  METHODS
  Forty-two healthy adult male Sprague-Dawley (SD) rats were randomly divided into 3 groups, the sham-operated control group (C), the SAP group (SAP), and the SSBE treated group (SSBE), 14 in each group. SAP induced ALl rat model was induced by retrograde injection of 5% sodium taurocholate (1 mL/kg) into the pancreatic duct. SSBE (100 m/kg) was administrated subcutaneously after the establishment of the SAP model. Equal dose of SSBE was injected again 12 h later. Equal volume of normal saline was administrated in the same way for rats in the C group and the SAP group. Rats were sacrificed after successful modeling and samples taken at 12 and 24 h. Pathological changes in the pancreas and the lung tissue were observed under light microscope. The ascites, serum amylase (AMS), wet/dry proportion (W/D) of the lung tissue, activities of myeloperoxidase (MPO), interleukin-1 (IL-1), IL-6, and tumor necrosis factor-alpha (TNF-alpha) were also measured.
  
  
  RESULTS
  Ascites and serum AMS activities significantly increased; MPO, IL-1, IL-6, TNF-alpha contents, and W/D ratio also significantly increased in the SAP group, when compared with the C group (P<0.05). Compared with the SAP group, those parameters were all attenuated in the SSBE group at 12 and 24 h (P<0.05, P<0.01). Pathological changes in the pancreas and the lung tissue were alleviated in the SSBE group under light microscope. The injury degree ranged between that of the C group and the SAP group.
  
  
  CONCLUSIONS
  SSBE could relieve the ALl in SAP model rats, which could be achieved through alleviating inflammation responses of SAP rats.
authors.forename: Mao-ming
authors.initials: MM
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.forename: Lin-hong
authors.initials: LH
authors.lastname: Yang
authors.forename: Le-wei
authors.initials: LW
authors.lastname: Liu
authors.forename: Xiao-cheng
authors.initials: XC
authors.lastname: Chen
authors.forename: Meng-tao
authors.initials: MT
authors.lastname: Zhou
authors.forename: Bi-cheng
authors.initials: BC
authors.lastname: Chen
mesh_terms: D055371:Acute Lung Injury / Q000188:drug therapy* / Q000209:etiology; D000818:Animals; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D007375:Interleukin-1; D015850:Interleukin-6; D008168:Lung; D008297:Male; D010179:Pancreas; D010195:Pancreatitis / Q000150:complications / Q000188:drug therapy*; D009195:Peroxidase; D051381:Rats; D017207:Rats, Sprague-Dawley; D031963:Sedum; D013656:Taurocholic Acid; D014409:Tumor Necrosis Factor-alpha
chemical_list: D004365:Drugs, Chinese Herbal; D007375:Interleukin-1; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D013656:Taurocholic Acid; D009195:Peroxidase