title: Quercetin ameliorates liver injury induced with Tripterygium glycosides by reducing oxidative stress and inflammation.
abstract: Quercetin (Que) is one of main compounds in Lysimachia christinae Hance (Christina loosestrife), and has both medicinal and nutritional value. Glycosides from Tripterygium wilfordii Hook.f. (léi gōng téng [the thunder duke vine]; TG) have diverse and broad bioactivities but with a high incidence of liver injury. Our previous study reported on the hepatoprotective properties of an ethanol extract from L. christinae against TG-induced liver injury in mice. This research is designed to observe, for the first time, the possible protective properties of the compound Que against TG-induced liver injury, and the underlying mechanisms that are involved in oxidative stress and anti-inflammation. The results indicated that TG caused excessive elevation in serum levels of alanine/aspartate transaminase (ALT/AST), alkaline phosphatase (ALP), gamma glutamyl transferase (γ-GT), and pro-inflammatory cytokine tumor necrosis factor-alpha (TNF-α), as well as hepatic lipid peroxidation (all P < 0.01). On the other hand, following TG exposure, we observed significantly reduced levels of biomarkers, including hepatic glutathione (GSH), glutathione-S-transferase (GST), glutathione peroxidase (GPx), and the anti-inflammatory cytokine interleukin (IL)-10, as well as the enzyme activity and mRNA expression of copper- and zinc-containing superoxide dismutase (CuZn-SOD) and catalase (CAT) (all P < 0.01). Nevertheless, all of these alterations were reversed by the pre-administration of Que or the drug bifendate (positive control) for 7 consecutive days. Therefore, this study suggests that Que ameliorates TG-induced acute liver injury, probably through its ability to reduce oxidative stress and its anti-inflammatory properties.
authors.affiliation: Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment & Chinese Medicine Development of Henan Province, Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China., College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, China.
authors.forename: Junming
authors.initials: J
authors.lastname: Wang
authors.forename: Mingsan
authors.initials: M
authors.lastname: Miao
authors.forename: Yueyue
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ruixin
authors.initials: R
authors.lastname: Liu
authors.forename: Xaobing
authors.initials: X
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Cui
authors.forename: Lingbo
authors.initials: L
authors.lastname: Qu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D015415:Biomarkers / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000378:metabolism; D006027:Glycosides / Q000009:adverse effects*; D007249:Inflammation / Q000188:drug therapy* / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*; D020011:Protective Agents / Q000494:pharmacology; D011794:Quercetin / Q000494:pharmacology*; D044967:Serum / Q000378:metabolism; D029757:Tripterygium / Q000009:adverse effects*
chemical_list: D000893:Anti-Inflammatory Agents; D015415:Biomarkers; D006027:Glycosides; D020011:Protective Agents; D011794:Quercetin
keywords: anti-inflammatory cytokines; antioxidant enzymes; cytokines anti-inflammatoires; cytokines pro-inflammatoires; enzymes antioxydantes; hepatoprotection; hépatoprotection; oxidative stress; pro-inflammatory cytokines; stress oxydant