title: Genotype-driven isolation of enterocin with novel bioactivities from mangrove-derived Streptomyces qinglanensis 172205.
abstract: The type II polyketide synthase (PKS) natural product enterocin (1) was isolated from a mangrove-derived novel species Streptomyces qinglanensis 172205 guided by genome sequence, and its putative biosynthetic gene cluster was revealed. Its natural analogues 5-deoxyenterocin (2) and wailupemycin A-C (3-5) were also identified by tandem mass spectrometry. By feeding experiments with aryl acids, strain 172205 was proved to incorporate partial exogenous starter units into enterocin- and wailupemycin-based analogues, thus being a new and suitable microorganism for engineering unnatural enc-derived polyketide metabolites. In addition, biological assays indicated that enterocin showed obvious inhibitory activity against β-amyloid protein (Aβ1-42) fibrillation and moderate cytotoxicity against HeLa and HepG2 for the first time.
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, School of Pharmaceutical Sciences, Wuhan University, Wuhan, 430071, China.
authors.forename: Dong-Bo
authors.initials: DB
authors.lastname: Xu
authors.forename: Min
authors.initials: M
authors.lastname: Ma
authors.forename: Zi-Xin
authors.initials: ZX
authors.lastname: Deng
authors.forename: Kui
authors.initials: K
authors.lastname: Hong
mesh_terms: D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors; D000970:Antineoplastic Agents / Q000378:metabolism; D053898:Biosynthetic Pathways / Q000235:genetics*; D001952:Bridged-Ring Compounds / Q000302:isolation & purification / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D004783:Environmental Microbiology; D005838:Genotype; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D013302:Streptomyces / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D016229:Amyloid beta-Peptides; D000970:Antineoplastic Agents; D001952:Bridged-Ring Compounds; C012306:enterocin