title: Resveratrol attenuates CXCL11 expression induced by proinflammatory cytokines in retinal pigment epithelial cells.
abstract: Dysfunction of the retinal pigment epithelium (RPE) resulting from chronic inflammation is implicated in the pathogenesis of age-related macular degeneration (AMD). RPE cells adjacent to drusen deposits in the AMD eye are known to contain CXCL11, a chemokine involved in inflammatory cell recruitment. We investigated the CXCL11 production by the human RPE (ARPE-19) cells under inflammatory conditions and tested its response to resveratrol, a naturally occurring anti-inflammatory antioxidant. A proinflammatory cytokine mixture consisting of IFN-γ, IL-1β and TNF-α highly increased CXCL11 mRNA expression and CXCL11 protein secretion by ARPE-19 cells. Resveratrol substantially inhibited the proinflammatory cytokines-induced CXCL11 production while partially blocking nuclear factor-κB activation. This inhibitory action of resveratrol was also observed for the cytokines-induced expression of chemokines CXCL9, CCL2 and CCL5. Our results indicate that resveratrol could potentially attenuate RPE inflammatory response implicated in the pathogenesis of AMD.
authors.affiliation: Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, United States. Electronic address: kuttyk@nei.nih.gov.
authors.forename: R Krishnan
authors.initials: RK
authors.lastname: Kutty
authors.affiliation: Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, United States.
authors.forename: William
authors.initials: W
authors.lastname: Samuel
authors.affiliation: Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, United States.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Abay
authors.affiliation: Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, United States.
authors.forename: Aswini
authors.initials: A
authors.lastname: Cherukuri
authors.affiliation: Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, United States.
authors.forename: Chandrasekharam N
authors.initials: CN
authors.lastname: Nagineni
authors.affiliation: Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, United States.
authors.forename: Todd
authors.initials: T
authors.lastname: Duncan
authors.affiliation: Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, United States.
authors.forename: Cynthia
authors.initials: C
authors.lastname: Jaworski
authors.affiliation: National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD 20892, United States.
authors.forename: Camasamudram
authors.initials: C
authors.lastname: Vijayasarathy
authors.affiliation: Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, United States.
authors.forename: T Michael
authors.initials: TM
authors.lastname: Redmond
mesh_terms: D002460:Cell Line; D054371:Chemokine CXCL11 / Q000276:immunology*; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000276:immunology; D006801:Humans; D007249:Inflammation / Q000188:drug therapy / Q000276:immunology / Q000473:pathology; D016328:NF-kappa B / Q000276:immunology*; D000077185:Resveratrol; D055213:Retinal Pigment Epithelium / Q000276:immunology* / Q000473:pathology; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: C515202:CXCL11 protein, human; D054371:Chemokine CXCL11; D016328:NF-kappa B; D013267:Stilbenes; D000077185:Resveratrol
keywords: Age-related macular degeneration; CXCL11; Nuclear factor-kappa B; Resveratrol; Retinal pigment epithelium