title: [AS-PCR amplification in identification of Sedum sarmentosum with its relative species].
abstract: OBJECTIVE
  To develop a scientific and effective method for identification of Sedum sarmentosum and its relative materials easily confused.
  
  
  METHODS
  DNA templates were extracted from plants, and DNA fragments of cpDNA trnL (tRNA-Leu) gene and trnL-trnF intergenic spacer were amplified and sequenced subsequently. trnL-trnF sequences of all samples were aligned.
  
  
  RESULTS
  The allele specific PCR method was studied with the designed allele-specific for distinguishing different samples. The result indicated that 300 bp and 500 bp DNA fragments were amplified from Sedum sarmentosum, where no any fragment was amplified from its relative species under the same reaction condition.
  
  
  CONCLUSIONS
  The primers designed in this study are specific for Sedum sarmentosum from its relative species.
authors.forename: Dong-mei
authors.initials: DM
authors.lastname: Xie
authors.forename: Lu-qi
authors.initials: LQ
authors.lastname: Huang
authors.forename: Min-jian
authors.initials: MJ
authors.lastname: Qin
authors.forename: De-qun
authors.initials: DQ
authors.lastname: Wang
authors.forename: Chao
authors.initials: C
authors.lastname: Jiang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
mesh_terms: D000483:Alleles; D017931:DNA Primers; D018742:DNA, Chloroplast / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D031963:Sedum / Q000145:classification*; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D018742:DNA, Chloroplast