title: Antioxidation and anti-inflammatory activity of Tang Bi Kang in rats with diabetic peripheral neuropathy.
abstract: BACKGROUND
  Tang Bi Kang (TBK) is a traditional Chinese medicine granule. It has been shown to have effects on nerve conduction velocity deficits, blood-related factors and oxidative stress. This study was undertaken to evaluate proposed antioxidative and anti-inflammatory activity of Tang Bi Kang in rats with diabetic peripheral neuropathy (DPN).
  
  
  METHODS
  DPN was induced in male Wistar rats by intraperitoneal administration of streptozocin (STZ) (60 mg/kg.b.w) for 8 weeks. Fasting blood glucose (FBG) levels were measured in the blood obtained by clipping the tails of the rats. Tail-flick tests were conducted with a tail-flick analgesic meter. Motor and sensory nerve conduction velocities (MNCV and SNCV) of sciatic nerve were measured directly at two sites using a Functional Experiment System. Oxidative stress makers such as malondialdehyde (MDA), superoxide-dismutase (SOD) and glutathione peroxidase (GSH-Px), inflammatory cytokines such as interleukin (IL)-6, and tumour necrosis factor (TNF)-α were estimated. The statistical analysis of results was carried out using Student t-test and one-way analysis of variance (ANOVA), followed by least-significant difference post hoc with SPSS.
  
  
  RESULTS
  The administration of TBK for 4 weeks in DPN rats resulted in a significant decrease in FBG levels compared to untreated DPN rats. There was a significant increase in MNCV and SNCV in the DPN rats compared to untreated DPN rats. Serum level of MDA was significantly reduced while the activities of SOD and GSH-pX were significantly increased in the TBK treated DPN rats. TBK prevented DPN-induced increase in the serum levels of IL-6 and TNF-α.
  
  
  CONCLUSIONS
  The results of this study demonstrate that the therapeutic effect of TBK on DPN rats may be associated with the antioxidative and anti-inflammatory responses.
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing, 100069, China. yxw0226@aliyun.com.|Beijing Key Lab of TCM Collateral Disease Theory Research, Beijing, 100069, China. yxw0226@aliyun.com.
authors.forename: Xin-Wei
authors.initials: XW
authors.lastname: Yang
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing, 100069, China. 424253640@qq.com.
authors.forename: Feng-Qi
authors.initials: FQ
authors.lastname: Liu
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing, 100069, China. 462707721@qq.com.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Guo
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing, 100069, China. 953715542@qq.com.|Beijing Key Lab of TCM Collateral Disease Theory Research, Beijing, 100069, China. 953715542@qq.com.
authors.forename: Wei-Jie
authors.initials: WJ
authors.lastname: Yao
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing, 100069, China. 330855009@qq.com.|Beijing Key Lab of TCM Collateral Disease Theory Research, Beijing, 100069, China. 330855009@qq.com.
authors.forename: Qing-Qin
authors.initials: QQ
authors.lastname: Li
authors.affiliation: The Graduate School, Beijing University of Chinese Medicine, Beijing, 100029, China. thliu@tom.com.
authors.forename: Tong-Hua
authors.initials: TH
authors.lastname: Liu
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing, 100069, China. xulp@ccmu.edu.cn.|Beijing Key Lab of TCM Collateral Disease Theory Research, Beijing, 100069, China. xulp@ccmu.edu.cn.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Xu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use*; D001786:Blood Glucose / Q000378:metabolism; D016207:Cytokines / Q000097:blood; D003929:Diabetic Neuropathies / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy* / Q000503:physiopathology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D007004:Hypoglycemic Agents / Q000494:pharmacology / Q000627:therapeutic use; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D009431:Neural Conduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000187:drug effects* / Q000502:physiology; D013311:Streptozocin; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D001786:Blood Glucose; D016207:Cytokines; D004365:Drugs, Chinese Herbal; D007004:Hypoglycemic Agents; D008315:Malondialdehyde; D013311:Streptozocin; D013482:Superoxide Dismutase