title: Toxicological Effect of Manganese on NF-κB/iNOS-COX-2 Signaling Pathway in Chicken Testes.
abstract: Manganese (Mn) pollution can cause tissue and organ dysfunction and structural damage. The toxicity of Mn in poultry was reported, but inflammatory damage that Mn induced in the testicular tissue has not been reported. The aim of this study was to investigate the effect of Mn poisoning on NF-κB/iNOS-COX-2 signaling pathway in chicken testes. One hundred eighty Hyline male chickens at 7 days of age were fed either commercial diet or MnCl2-added commercial diet containing 600, 900, and 1800 mg/kg Mn for 30, 60, and 90 days, respectively. The messenger RNA (mRNA) expression of nuclear factor-κB (NF-κB), tumor necrosis factor-α (TNF-α), cyclooxygenase-2 (COX-2), and inducible nitric oxide synthase (iNOS), nitric oxide (NO) content, iNOS activity, and histopathology were examined in chicken testes. The results showed that excess Mn upregulated mRNA expression of NF-κB, COX-2, TNF-α, and iNOS, NO content, and iNOS activity at 60th and 90th day. Mn had a time-dependent effect on NF-κB and TNF-α mRNA expression. Mn had a dose- and time-dependent effect on NO content and iNOS activity. Mn exposure induced chicken testis histological changes in dose- and time-dependent manner. It indicated that Mn exposure resulted in inflammatory injury of chicken testis tissue through NF-κB/iNOS-COX-2 signaling pathway.
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Du
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
authors.forename: Yihao
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Heilongjiang Grassland and Forage Central Experimental Station, Harbin, 150069, People's Republic of China. txj651007@163.com.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Teng
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
authors.forename: Kun
authors.initials: K
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, People's Republic of China. tengxiaohua@neau.edu.cn.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Teng
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China. lishu@neau.edu.cn.
authors.forename: Shu
authors.initials: S
authors.lastname: Li
mesh_terms: D000818:Animals; D002645:Chickens; D051546:Cyclooxygenase 2 / Q000096:biosynthesis / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D007249:Inflammation / Q000139:chemically induced / Q000473:pathology; D008297:Male; D008345:Manganese / Q000633:toxicity*; D016328:NF-kappa B / Q000096:biosynthesis / Q000187:drug effects*; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis / Q000187:drug effects*; D015398:Signal Transduction / Q000187:drug effects*; D013737:Testis / Q000473:pathology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D008345:Manganese; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2
keywords: Chicken; Manganese; NF-κB/iNOS-COX-2 signaling pathway; Testis