title: Japonicone A inhibits the growth of non-small cell lung cancer cells via mitochondria-mediated pathways.
abstract: Japonicone A, which is a natural product isolated from the aerial part of Inula japonica Thunb., has a wide range of clinical applications, including anti-inflammation and anti-oxidation. This study investigated the effects of japonicone A on the growth of non-small cell lung cancer (NSCLC) cell lines. The results showed that japonicone A significantly inhibited the growth of NSCLC cell lines in a dose- and time-dependent manner. This product also blocked cell cycle progression at S phase and induced mitochondrial-related apoptosis by upregulating Bax, cleaved caspase-9, cleaved caspase-3, and cleaved poly(ADP-ribose) polymerase (PARP) protein levels and by downregulating Bcl-2, cyclin D1, CDC25A, and CDK2 protein levels. In vivo, japonicone A suppressed tumor growth via the same mechanism as that observed in vitro. In conclusion, our study is the first to report that japonicone A has an inhibitory effect on the growth of NSCLC cells, indicating that japonicone A administration is a potential therapeutic approach for future NSCLC treatments.
authors.affiliation: Department of Respiratory Medicine, Shanxi Medical University Second Hospital, Taiyuan, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Du
authors.affiliation: Department of Respiratory Medicine, Shanxi Medical University Second Hospital, Taiyuan, People's Republic of China.
authors.forename: Jiannan
authors.initials: J
authors.lastname: Gong
authors.affiliation: Department of Respiratory Medicine, Shanxi Medical University Second Hospital, Taiyuan, People's Republic of China.
authors.forename: Xinrui
authors.initials: X
authors.lastname: Tian
authors.affiliation: Department of Respiratory Medicine, Shanxi Medical University Second Hospital, Taiyuan, People's Republic of China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Yan
authors.affiliation: Department of Respiratory Medicine, Shanxi Medical University Second Hospital, Taiyuan, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Guo
authors.affiliation: Department of Respiratory Medicine, Shanxi Medical University Second Hospital, Taiyuan, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Huang
authors.affiliation: Department of General Surgery, Shanxi Medical University Second Hospital, Taiyuan, People's Republic of China.
authors.forename: Bingtai
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Respiratory Medicine, Shanxi Medical University Second Hospital, Taiyuan, People's Republic of China.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Hu
authors.affiliation: Department of Respiratory Medicine, Shanxi Medical University Second Hospital, Taiyuan, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Respiratory Medicine, Shanxi Medical University Second Hospital, Taiyuan, People's Republic of China.
authors.forename: Yinping
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Respiratory Medicine, Shanxi Medical University Second Hospital, Taiyuan, People's Republic of China. ljqhx@sina.com.
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of General Surgery, Shanxi Medical University Second Hospital, Taiyuan, People's Republic of China. limaolan6@163.com.|Department of General Surgery and Laboratory of General Surgery, Xinhua Hospital affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China. limaolan6@163.com.|Institute of Biliary Tract Disease, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China. limaolan6@163.com.
authors.forename: Maolan
authors.initials: M
authors.lastname: Li
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D007124:Immunoenzyme Techniques; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D045787:Sesquiterpenes, Eudesmane / Q000494:pharmacology*; D045789:Sesquiterpenes, Guaiane / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D045787:Sesquiterpenes, Eudesmane; D045789:Sesquiterpenes, Guaiane; D051028:bcl-2-Associated X Protein; C585467:japonicone A; D011065:Poly(ADP-ribose) Polymerases; D053148:Caspase 3; D053453:Caspase 9
keywords: Apoptosis; Cell cycle arrest; Japonicone A; Mitochondria-mediated pathways; Non-small cell lung cancer