title: Generation and characterization of PDGFRα-GFPCreERT2 knock-In mouse line.
abstract: Platelet-derived growth factor (PDGF) and its receptor play an important role in embryogenesis. PDGF receptor α (PDGFRα) is expressed specifically in the embryonic day 7.5 (E7.5) mesoderm and in the E9.5 neural crest among other tissues. PDGFRα-expressing cells and their descendants are involved in the formation of various tissues. To trace PDGFRα-expressing cells in vivo, we generated a knock-in mouse line that expressed a fusion protein of green fluorescent protein (GFP), Cre recombinase (Cre), and mutated estrogen receptor ligand-binding domain (ERT2) under the control of the PDGFRα promoter. In these mice, Cre activity in PDGFRα-expressing cells could be induced by tamoxifen treatment. Taken together, our results suggest that the knock-in mouse line generated here could be useful for studying PDGFRα-expressing cells and their descendants in vivo at various stages of development.
authors.affiliation: Department of Cell Modulation, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Miwa
authors.affiliation: Department of Cell Modulation, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto, Japan.
authors.forename: Takumi
authors.initials: T
authors.lastname: Era
mesh_terms: D000818:Animals; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D055879:Gene Knock-In Techniques; D023061:Gene Order; D018390:Gene Targeting; D017930:Genes, Reporter; D056426:Genetic Loci; D049452:Green Fluorescent Proteins / Q000235:genetics* / Q000378:metabolism; D019426:Integrases / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D054730:Protein Interaction Domains and Motifs / Q000235:genetics*; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000235:genetics* / Q000378:metabolism; D011960:Receptors, Estrogen / Q000737:chemistry / Q000235:genetics*; D019076:Transgenes
chemical_list: D011960:Receptors, Estrogen; D049452:Green Fluorescent Proteins; D020796:Receptor, Platelet-Derived Growth Factor alpha; C045073:Cre recombinase; D019426:Integrases
keywords: CreERT2; PDGFRα; knock-in; mesenchyme; mesoderm; neural crest