title: Engineering Corynebacterium crenatum to produce higher alcohols for biofuel using hydrolysates of duckweed (Landoltia punctata) as feedstock.
abstract: Early trials have demonstrated great potential for the use of duckweed (family Lemnaceae) as the next generation of energy plants for the production of biofuels. Achieving this technological advance demands research to develop novel bioengineering microorganisms that can ferment duckweed feedstock to produce higher alcohols. In this study, we used relevant genes to transfer five metabolic pathways of isoleucine, leucine and valine from the yeast Saccharomyces cerevisiae into the bioengineered microorganism Corynebacterium crenatum. Experimental results showed that the bioengineered strain was able to produce 1026.61 mg/L of 2-methyl-1-butanol by fermenting glucose, compared to 981.79 mg/L from the acid hydrolysates of duckweed. The highest isobutanol yields achieved were 1264.63 mg/L from glucose and 1154.83 mg/L from duckweed, and the corresponding highest yields of 3-methyl-1-butanol were 748.35 and 684.79 mg/L. Our findings demonstrate the feasibility of using bioengineered C. crenatum as a platform to construct a bacterial strain that is capable of producing higher alcohols. We have also shown the promise of using duckweed as the basis for developing higher alcohols, illustrating that this group of plants represents an ideal fermentation substrate that can be considered the next generation of alternative energy feedstocks.
authors.affiliation: Key Laboratory of Bio-resources and Eco-environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610064, Sichuan, PR China. 44036872@qq.com.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Su
authors.affiliation: Key Laboratory of Bio-resources and Eco-environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610064, Sichuan, PR China. 173619060@qq.com.
authors.forename: Juan
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Bio-resources and Eco-environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610064, Sichuan, PR China. 971541278@qq.com.
authors.forename: Qiuli
authors.initials: Q
authors.lastname: Lu
authors.affiliation: Key Laboratory of Bio-resources and Eco-environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610064, Sichuan, PR China. 975805758@qq.com.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Bio-resources and Eco-environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610064, Sichuan, PR China. xietianxietian12@qq.com.
authors.forename: Tian
authors.initials: T
authors.lastname: Xie
authors.affiliation: Bioenergy Laboratory, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, Sichuan, PR China. zhaohai@cib.ac.cn.
authors.forename: Hai
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Bio-resources and Eco-environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610064, Sichuan, PR China. mlwang@scu.edu.cn.
authors.forename: Maolin
authors.initials: M
authors.lastname: Wang
mesh_terms: D000438:Alcohols / Q000378:metabolism*; D029064:Araceae / Q000737:chemistry*; D056804:Biofuels; D000440:Butanols / Q000378:metabolism; D003352:Corynebacterium / Q000235:genetics / Q000378:metabolism*; D005285:Fermentation; D015870:Gene Expression; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D007532:Isoleucine / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D060847:Metabolic Engineering / Q000379:methods*; D053858:Metabolic Networks and Pathways / Q000235:genetics; D010936:Plant Extracts / Q000378:metabolism*; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D013213:Starch / Q000378:metabolism; D014633:Valine / Q000378:metabolism
chemical_list: D000438:Alcohols; D056804:Biofuels; D000440:Butanols; D010936:Plant Extracts; D029701:Saccharomyces cerevisiae Proteins; D007532:Isoleucine; C040507:isobutyl alcohol; D013213:Starch; D007930:Leucine; D014633:Valine; D005947:Glucose