title: Lipid molecules influence early stages of yeast SNARE-mediated membrane fusion.
abstract: Lipid molecules, structural components of biomembranes, have been proposed for an important role in membrane fusion. Through various techniques based on a protein-reconstituted vesicle-vesicle fusion system, we investigated the influence of several lipid molecules on different stages of a yeast soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE)-mediated membrane fusion process. Lipid compositions played a significant role in the early stages, docking and lipid mixing, while only exhibiting a minor effect on fusion pore formation and dilation phases, indicated by both small and large content mixing.
authors.affiliation: Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA 50011, USA.
authors.forename: Ying
authors.initials: Y
authors.lastname: Lai
authors.forename: Lin
authors.initials: L
authors.lastname: Zhao
authors.forename: Bing
authors.initials: B
authors.lastname: Bu
authors.forename: Xiaochu
authors.initials: X
authors.lastname: Lou
authors.forename: Dechang
authors.initials: D
authors.lastname: Li
authors.forename: Baohua
authors.initials: B
authors.lastname: Ji
authors.forename: Jiankang
authors.initials: J
authors.lastname: Liu
authors.forename: Jiajie
authors.initials: J
authors.lastname: Diao
authors.forename: Yeon-Kyun
authors.initials: YK
authors.lastname: Shin
mesh_terms: D050356:Lipid Metabolism; D008561:Membrane Fusion; D050600:SNARE Proteins / Q000378:metabolism*; D015003:Yeasts / Q000378:metabolism*
chemical_list: D050600:SNARE Proteins