title: Relationship between HPV 16, 18, 31, 33, 45 DNA detection and quantitation and E6/E7 mRNA detection among a series of cervical specimens with various degrees of histological lesions.
abstract: Better understanding of the correlation between high-risk HPV DNA testing, viral load quantitation, and E6/E7 mRNA detection is required. The aim of this study was to assess the relationship between these markers and the severity of cervical lesions. One-hundred and fifty one directed cervical specimens were analysed (normal, cervical intraepithelial neoplasia, and cancer). HPV types 16, 18, 31, 33, and 45 DNA detection and quantititation and E6/E7 mRNA detection were performed. DNA was detected in 87 (57.6%) samples and increased from 0% (normal) to 93.9% (cancer). E6/E7 mRNA was detected in 65 (43%) samples and increased with the severity of the lesions from 0% (normal) to 78.8% (26/33) (cancers) (P < 0.001). HPV DNA and E6/E7 mRNA detection were compared in the 141 samples harbouring HPV16, 18, 31, 33, or 45 infection: 45.4% (64/141) of specimens were DNA-/mRNA-, 46% (65/141) were DNA + /mRNA+ and 8.5% (12/141) were DNA + /mRNA-. The proportion of DNA + /mRNA+ specimens increased with the severity of the lesions (P < 0.001). All normal cervix specimens were DNA-/mRNA-. Among grade 2 cervical intraepithelial neoplasia, prevalence of DNA was higher than that of mRNA: 41.6% (5/12) versus 25% (3/12), whereas it was 79.3% (46/58) versus 62% (36/58) among grade 3 cervical intraepithelial neoplasia. Full concordance was observed in cancers as all the 26 DNA+ specimens were mRNA +. Median overall HPV load was higher in DNA + /mRNA+ than in DNA + /mRNA- specimens (1.41 × 10(6) vs. 9.1 × 10(2) copies per million cells, P < 0.001). Both E6/E7 mRNA detection and concordant DNA + /mRNA+ detection increases with the severity of the lesions and with the HPV DNA load.
authors.affiliation: Federation of Clinical Microbiology, URMITE UMR CNRS 7278 IRD 198 IHU Infection Méditerranée, Department of Infectious and Tropical Diseases, CHU Timone, Marseille, France.
authors.forename: Carolina
authors.initials: C
authors.lastname: Baron
authors.affiliation: Federation of Clinical Microbiology, URMITE UMR CNRS 7278 IRD 198 IHU Infection Méditerranée, Department of Infectious and Tropical Diseases, CHU Timone, Marseille, France.
authors.forename: Mireille
authors.initials: M
authors.lastname: Henry
authors.affiliation: Federation of Clinical Microbiology, URMITE UMR CNRS 7278 IRD 198 IHU Infection Méditerranée, Department of Infectious and Tropical Diseases, CHU Timone, Marseille, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Tamalet
authors.affiliation: Department of Pathology, Assistance Publique des Hôpitaux de Marseille (APHM), Hôpital Nord, Marseille, France.
authors.forename: Julia
authors.initials: J
authors.lastname: Villeret
authors.affiliation: Federation of Clinical Microbiology, URMITE UMR CNRS 7278 IRD 198 IHU Infection Méditerranée, Department of Infectious and Tropical Diseases, CHU Timone, Marseille, France.
authors.forename: Herve
authors.initials: H
authors.lastname: Richet
authors.affiliation: Department of Obstetrics and Gynaecology, Hôpital Nord, Assistance Publique des Hôpitaux de Marseille (APHM), Aix-Marseille Université (AMU), CNRS, IRD, Avignon Université, Marseille, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Carcopino
mesh_terms: D000328:Adult; D000368:Aged; D003430:Cross-Sectional Studies; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008875:Middle Aged; D009856:Oncogene Proteins, Viral / Q000096:biosynthesis* / Q000235:genetics; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000150:complications* / Q000473:pathology* / Q000821:virology; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D012720:Severity of Illness Index; D002583:Uterine Cervical Neoplasms / Q000473:pathology* / Q000821:virology
chemical_list: D009856:Oncogene Proteins, Viral; D012333:RNA, Messenger; D012367:RNA, Viral
keywords: E6E7 mRNA; cervical intraepithelial neoplasia; genotyping; high-risk HPV load; papillomavirus