title: Validated UPLC-MS/MS method for determination of hordenine in rat plasma and its application to pharmacokinetic study.
abstract: Hordenine is an active compound found in several foods, herbs and beer. In this work, a sensitive and selective UPLC-MS/MS method for determination of hordenine in rat plasma was developed. After addition of caulophylline as internal standard (IS), protein precipitation by acetonitrile-methanol (9:1, v/v) was used as sample preparation. Chromatographic separation was achieved on a UPLC BEH HILIC (2.1 mm × 100 mm, 1.7 μm) with acetonitrile (containing 10mM ammonium formate) and water (containing 0.1% formic acid and 10 mM ammonium formate) as mobile phase with gradient elution. An electrospray ionization source was applied and operated in positive ion mode; multiple reaction monitoring (MRM) mode was used for quantification using target fragment ions m/z 166.1 → 121.0 for hordenine and m/z 205.1 → 58.0 for IS. Calibration plots were linear over the range of 2-2000 ng/mL for hordenine in rat plasma. Mean recoveries of hordenine in rat plasma were in the range of 80.4-87.3%. RSD of intra-day and inter-day precision were both <8%. The accuracy of the method ranged from 97.0% to 107.7%. The method was successfully applied to pharmacokinetic study of hordenine after oral and intravenous administration.
authors.affiliation: School of Basic Medicine, Wenzhou Medical University, 325035 Wenzhou, China.
authors.forename: Jianshe
authors.initials: J
authors.lastname: Ma
authors.affiliation: The Laboratory of Clinical Pharmacy, The People's Hospital of Lishui, 323000 Lishui, China.
authors.forename: Shuanghu
authors.initials: S
authors.lastname: Wang
authors.affiliation: Analytical and Testing Center, Wenzhou Medical University, 325035 Wenzhou, China.
authors.forename: Xueli
authors.initials: X
authors.lastname: Huang
authors.affiliation: The Laboratory of Clinical Pharmacy, The People's Hospital of Lishui, 323000 Lishui, China.
authors.forename: Peiwu
authors.initials: P
authors.lastname: Geng
authors.affiliation: Analytical and Testing Center, Wenzhou Medical University, 325035 Wenzhou, China.
authors.forename: Congcong
authors.initials: C
authors.lastname: Wen
authors.affiliation: The Laboratory of Clinical Pharmacy, The People's Hospital of Lishui, 323000 Lishui, China.
authors.forename: Yunfang
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: School of Basic Medicine, Wenzhou Medical University, 325035 Wenzhou, China; Forensic Center, Wenzhou Medical University, 325000 Wenzhou, China. Electronic address: yls@wmu.edu.cn.
authors.forename: Linsheng
authors.initials: L
authors.lastname: Yu
authors.affiliation: Analytical and Testing Center, Wenzhou Medical University, 325035 Wenzhou, China; Forensic Center, Wenzhou Medical University, 325000 Wenzhou, China. Electronic address: lankywang@163.com.
authors.forename: Xianqin
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004355:Drug Stability; D057230:Limit of Detection; D008297:Male; D010949:Plasma / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods; D014439:Tyramine / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry
chemical_list: C007964:hordenine; D014439:Tyramine
keywords: HILIC; Hordenine; Pharmacokinetics; Rat plasma; UPLC–MS/MS