title: Nosocomial Intravascular Catheter Infections with Extended-spectrum Beta-lactamase-producing Escherichia coli in Calves after Strain Introduction from a Commercial Herd.
abstract: An outbreak of intravascular catheter-related infections by extended-spectrum β-lactamase (ESBL)-producing Escherichia coli in calves in an animal teaching hospital is reported. Pulsed-field gel electrophoresis was used for strain typing to determine the origin and dissemination of these strains. All 19 strains harboured the blaCTX-M-14,and six strains also overexpressed their chromosomal AmpC gene. Evidence on the introduction of the strain from a beef herd, experiencing neonatal diarrhoea and increased mortality, to the clinic through admission of diarrhoeic calves was provided. Strains isolated from phlebitis cases from other herds up to 5 months later showed a high similarity with the initial strain, suggesting that the strain had become nosocomial. The catheter infections with ESBL/AmpC-producing E. coli resulted in a prolonged hospitalization, increased anti-microbial use and mortality. This report points towards the potential dangers of the emergence of ESBL/AmpC-producing bacteria in susceptible food animals and warns farmers and veterinarians for the facility by which they are introduced into another environment.
authors.affiliation: Department of Large Animal Internal Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
authors.forename: B
authors.initials: B
authors.lastname: Pardon
authors.affiliation: Department of Bacteriology, Pathology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Smet
authors.affiliation: Department of Bacteriology, Pathology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.|Department of Bacteriology and Immunology, CODA-CERVA-VAR, Brussels, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: Butaye
authors.affiliation: Department of Bacteriology and Immunology, CODA-CERVA-VAR, Brussels, Belgium.
authors.forename: M A
authors.initials: MA
authors.lastname: Argudín
authors.affiliation: Department of Large Animal Internal Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
authors.forename: B
authors.initials: B
authors.lastname: Valgaeren
authors.affiliation: Health Care Associated Infections and Antimicrobial Resistance, Scientific Institute of Public Health (WIV-ISP), Brussels, Belgium.
authors.forename: B
authors.initials: B
authors.lastname: Catry
authors.affiliation: Department of Bacteriology, Pathology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
authors.forename: F
authors.initials: F
authors.lastname: Haesebrouck
authors.affiliation: Department of Large Animal Internal Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: Deprez
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D057785:Catheters / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D003428:Cross Infection / Q000382:microbiology / Q000662:veterinary*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary; D004926:Escherichia coli / Q000235:genetics / Q000502:physiology*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D006762:Hospitals, Animal; D006784:Hospitals, Teaching; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; C048795:AmpC beta-lactamases; D001618:beta-Lactamases
keywords: 
  Escherichia coli
  ; 
  PFGE
  ; ESBLs; cattle; intravascular catheter; phlebitis