title: Chemical composition and insecticidal activity of Cymbopogon citratus essential oil from Cuba and Brazil against housefly.
abstract: Essential oil of Cymbopogon citratus collected from Brazil and Cuba was tested to a chemical characterization and then was tested on the post-embryonic development of Musca domestica. The chemical composition analysis by GC-MS of the oils from Brazil/Cuba allowed the identification of 13 and 12 major constituents respectively; nine of them common to both. In the both oils, the main components were the isomers geranial and neral, which together form the compound citral. This corresponds to a total of 97.92%/Brazil and 97.69%/Cuba of the compounds identified. The monoterpene myrcene, observed only in the sample of Cuba, presented a large relative abundance (6.52%). The essential oil of C. citratus (Brazil/Cuba) was dissolved in DMSO and tested at concentrations of 5, 10, 25, 50, 75 and 100% and citral was prepared by mixing 16.8 mg with 960 µL DMSO. Both essential oils and monoterpene citral were applied topically to newly-hatched larvae (1µL/larva). The results showed a lethal concentration (LC50) of 4.25 and 3.24% for the Brazilian and Cuban essential oils, respectively. Mortalities of larval and newly-hatched larvae to adult periods were dose-dependent for the two both oils as for monoterpene citral, reaching 90%. Both essential oils and citral caused morphological changes in adult specimens.
authors.affiliation: Laboratório de Educação em Ambiente e Saúde, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Zeneida Teixeira
authors.initials: ZT
authors.lastname: Pinto
authors.affiliation: Laboratorio de Aseguramiento de la Calidad, Moa, Cuba.
authors.forename: Félix Fernández
authors.initials: FF
authors.lastname: Sánchez
authors.affiliation: Laboratório de Plantas Medicinais e Derivados, Farmanguinhos, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Arith Ramos
authors.initials: AR
authors.lastname: dos Santos
authors.affiliation: Laboratório de Plantas Medicinais e Derivados, Farmanguinhos, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Ana Claudia Fernandes
authors.initials: AC
authors.lastname: Amaral
authors.affiliation: Laboratório de Plantas Medicinais e Derivados, Farmanguinhos, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: José Luiz Pinto
authors.initials: JL
authors.lastname: Ferreira
authors.affiliation: Departamento de Farmacia, Universidad de Oriente, Santiago de Cuba, Cuba.
authors.forename: Julio César
authors.initials: JC
authors.lastname: Escalona-Arranz
authors.affiliation: Laboratório de Transmissores de Leishmanioses, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Margareth Maria de Carvalho
authors.initials: MM
authors.lastname: Queiroz
mesh_terms: D000818:Animals; D001938:Brazil; D003462:Cuba; D031743:Cymbopogon; D006793:Houseflies / Q000187:drug effects*; D007306:Insecticides; D009822:Oils, Volatile / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D007306:Insecticides; D009822:Oils, Volatile