title: Modulation of (14) C-labeled glucose metabolism by zinc during aluminium induced neurodegeneration.
abstract: Aluminium (Al) is one of the most prominent metals in the environment and is responsible for causing several neurological disorders, including Alzheimer's disease. On the other hand, zinc (Zn) is an essential micronutrient that is involved in regulating brain development and function. The present study investigates the protective potential of Zn in the uptake of (14) C-labeled amino acids and glucose and their turnover in rat brain slices during Al intoxication. Male Sprague Dawley rats (140-160 g) were divided into four different groups: normal control, Al treated (100 mg/kg body weight/day via oral gavage), Zn treated (227 mg/liter in drinking water), and Al + Zn treated. Radiorespirometric assay revealed an increase in glucose turnover after Al exposure that was attenuated after Zn treatment. Furthermore, the uptake of (14) C-labeled glucose was increased after Al treatment but was appreciably decreased upon Zn supplementation. In addition, the uptakes of (14) C-lysine, (14) C-leucine, and (14) C-aspartic acid were also found to be elevated following Al exposure but were decreased after Zn treatment. Al treatment also caused alterations in the neurohistoarchitecture of the brain, which were improved after Zn coadministration. Therefore, the present study suggests that Zn provides protection against Al-induced neurotoxicity by regulating glucose and amino acid uptake in rats, indicating that Zn could be a potential candidate for the treatment of various neurodegenerative disorders.
authors.affiliation: Department of Biophysics, Panjab University, Chandigarh, India.
authors.forename: Neha
authors.initials: N
authors.lastname: Singla
authors.affiliation: Department of Biophysics, Panjab University, Chandigarh, India.
authors.forename: D K
authors.initials: DK
authors.lastname: Dhawan
mesh_terms: D000535:Aluminum / Q000633:toxicity*; D000596:Amino Acids / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D050260:Carbohydrate Metabolism / Q000187:drug effects*; D002247:Carbon Isotopes / Q000378:metabolism; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D004195:Disease Models, Animal; D005947:Glucose / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D019636:Neurodegenerative Diseases / Q000139:chemically induced*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015032:Zinc / Q000494:pharmacology*
chemical_list: D000596:Amino Acids; D002247:Carbon Isotopes; D000535:Aluminum; D005947:Glucose; D015032:Zinc
keywords: 14C-labeled glucose; aluminium; amino acids; cerebellum; cerebrum; histology; neurodegeneration; zinc