title: Dual-Modality Optical/PET Imaging of PARP1 in Glioblastoma.
abstract: OBJECTIVE
  The current study presents [(18)F]PARPi-FL as a bimodal fluorescent/positron emission tomography (PET) agent for PARP1 imaging.
  
  
  METHODS
  [(18)F]PARPi-FL was obtained by (19)F/(18)F isotopic exchange and PET experiments, biodistribution studies, surface fluorescence imaging, and autoradiography carried out in a U87 MG glioblastoma mouse model.
  
  
  RESULTS
  [(18)F]PARPi-FL showed high tumor uptake in vivo and ex vivo in small xenografts (< 2 mm) with both PET and optical imaging technologies. Uptake of [(18)F]PARPi-FL in blocked U87 MG tumors was reduced by 84 % (0.12 ± 0.02 %injected dose/gram (%ID/g)), showing high specificity of the binding. PET imaging showed accumulation in the tumor (1 h p.i.), which was confirmed by ex vivo phosphor autoradiography.
  
  
  CONCLUSIONS
  The fluorescent component of [(18)F]PARPi-FL enables cellular resolution optical imaging, while the radiolabeled component of [(18)F]PARPi-FL allows whole-body deep-tissue imaging of malignant growth.
authors.affiliation: Department of Radiology, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY, 10065, USA.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Carlucci
authors.affiliation: Department of Radiology, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY, 10065, USA.|Department of Chemistry and Biochemistry, Hunter College of the City University of New York, New York, NY, 10065, USA.|Ph.D. Program in Chemistry, The Graduate Center of the City University of New York, New York, 10018, USA.
authors.forename: Brandon
authors.initials: B
authors.lastname: Carney
authors.affiliation: Department of Radiology, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY, 10065, USA.
authors.forename: Christian
authors.initials: C
authors.lastname: Brand
authors.affiliation: Department of Radiology, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY, 10065, USA.
authors.forename: Susanne
authors.initials: S
authors.lastname: Kossatz
authors.affiliation: Department of Radiology, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY, 10065, USA.
authors.forename: Christopher P
authors.initials: CP
authors.lastname: Irwin
authors.affiliation: Department of Radiology, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY, 10065, USA.
authors.forename: Sean D
authors.initials: SD
authors.lastname: Carlin
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital, Boston, MA, 02114, USA.
authors.forename: Edmund J
authors.initials: EJ
authors.lastname: Keliher
authors.affiliation: Department of Radiology, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY, 10065, USA.|Weill Cornell Medical College, New York, NY, 10065, USA.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Weber
authors.affiliation: Department of Radiology, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY, 10065, USA. reinert@mskcc.org.|Weill Cornell Medical College, New York, NY, 10065, USA. reinert@mskcc.org.
authors.forename: Thomas
authors.initials: T
authors.lastname: Reiner
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology; D001932:Brain Neoplasms / Q000201:enzymology* / Q000473:pathology; D016022:Case-Control Studies; D005453:Fluorescence; D005909:Glioblastoma / Q000201:enzymology* / Q000473:pathology; D006207:Half-Life; D064593:Heterografts; D006801:Humans; D051379:Mice; D064847:Multimodal Imaging; D061848:Optical Imaging; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D049268:Positron-Emission Tomography
chemical_list: C499321:Parp1 protein, mouse; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases
keywords: Fluorescence; Glioblastoma; Imaging; Multimodality; PARP1; PET; U87 MG