title: [Human soluble dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin inhibits phagocytosis of Staphylococcus aureus by immature dendritic cells].
abstract: OBJECTIVE
  To study the effect and mechanism of soluble dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (sDC-SIGN) on the phagocytosis of Staphylococcus aureus (S. aureus) by immature dendritic cells (imDCs).
  
  
  METHODS
  Flow cytometry was employed to examine the effect of sDC-SIGN on the phagocytosis of S. aureus by imDCs. Enzyme-linked immunosorbent assay (ELISA) was used to analyze the binging of sDC-SIGN to S. aureus, lipoteichoic acid (LTA) and lipopolysaccharides (LPS) and investigate the effect of the ligands mannan and LTA and anti-DC-SIGN antibodies 1C6 and 4H3 on the binging of sDC-SIGN to S. aureus.
  
  
  RESULTS
  sDC-SIGN inhibited the phagocytosis of S. aureus by imDCs. sDC-SIGN bound to S. aureus in a Ca(2+)-dependent manner. sDC-SIGN concentration-dependently bound to LTA, but not to LTA, and the binging of sDC-SIGN to S. aureus was blocked by mannan, LTA, 1C6 and 4H3.
  
  
  CONCLUSIONS
  sDC-SIGN preferentially binds to the carbohydrate constituents on S. aureus to affect the binding between membrane-bound DC-SIGN and S. aureus, thus suppressing the phagocytosis of S. aureus by imDCs.
authors.affiliation: Department of Immunology, Southern Medical University, Guangzhou 510515, China. E-mail: 316135472@qq.com.
authors.forename: Hui-Jie
authors.initials: HJ
authors.lastname: Li
authors.forename: Tian-Yu
authors.initials: TY
authors.lastname: Xu
authors.forename: Jia
authors.initials: J
authors.lastname: Zhou
authors.forename: Ling-Yan
authors.initials: LY
authors.lastname: Zhu
authors.forename: Li-Yun
authors.initials: LY
authors.lastname: Zhang
authors.forename: Xiao
authors.initials: X
authors.lastname: Lu
authors.forename: Zheng-Liang
authors.initials: ZL
authors.lastname: Chen
mesh_terms: D015815:Cell Adhesion Molecules / Q000378:metabolism*; D003713:Dendritic Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D037181:Lectins, C-Type / Q000378:metabolism*; D008070:Lipopolysaccharides; D010587:Phagocytosis; D011956:Receptors, Cell Surface / Q000378:metabolism*; D013211:Staphylococcus aureus; D013682:Teichoic Acids; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D015815:Cell Adhesion Molecules; D037181:Lectins, C-Type; D008070:Lipopolysaccharides; D011956:Receptors, Cell Surface; D013682:Teichoic Acids; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin; C009900:lipoteichoic acid