title: Structural elucidation of two new megastigmane glycosides from the leaves of Aquilaria sinensis.
abstract: The present study was designed to determine the chemical constituents and identify new components of the leaves of Aquilaria sinensis (Lour.) Gilg. The compounds were isolated and purified by repeated silica gel, Sephadex LH-20, and ODS column chromatography and their structures were elucidated by NMR and HR-ESI-MS spectrometry. Eight megastigmane glycosides and two cucurbitacins were isolated and identified as (9S) megastigma-4,7-diene-2,3,9-triol 9-O-β-D-glucopyranoside (1), (9S) megastigma-4(13),7-diene-3,6,9-triol 9-O-β-D-glucopyranoside (2), macarangloside D (3), corchoionoside C (4), staphylionoside H (5), (+) 3-oxo-α-ionol-β-D-glucopyranoside (6), (-) 3-oxo-α-ionol-β-D-glucopyranoside (7), citroside B (8), 2-O-β-D-glucopyranosyl cucurbitacin I (9), bryoamaride (10). Compounds 1 and 2 were newly identified megstigmane glucosides and reported from this genus for the first time.
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Sun
authors.affiliation: TaiJi Pharmaceutical Research Institute, Chongqing 400118, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Xia
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
authors.affiliation: Guangdong Junyuan Pharmaceutical Co., Ltd., Guangzhou 525432, China.
authors.forename: Ke-Yuan
authors.initials: KY
authors.lastname: Wang
authors.affiliation: Guangdong Junyuan Pharmaceutical Co., Ltd., Guangzhou 525432, China.
authors.forename: Jin-Ming
authors.initials: JM
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China. Electronic address: pengfeitu@vip.163.com.
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Tu
mesh_terms: D002853:Chromatography, Liquid; D054728:Cucurbitacins / Q000737:chemistry; D003512:Cyclohexanones / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D045792:Norisoprenoids / Q000737:chemistry* / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry*; D029645:Thymelaeaceae / Q000737:chemistry*
chemical_list: C000604044:(9S) megastigma-4(13),7-diene-3,6,9-triol 9-O-beta-D-glucopyranoside; C000604043:(9S)megastigma-4,7-diene-2,3,9-triol 9-O-beta-D-glucopyranoside; D003512:Cyclohexanones; D005960:Glucosides; D045792:Norisoprenoids; C413264:megastigmane; D054728:Cucurbitacins
keywords: Aquilaria sinensis (Lour.) Gilg; Megstigmane glucosides