title: Protective effect of thymoquinone against testicular torsion induced oxidative injury.
abstract: We aimed to determine the protective effects of thymoquinone (TQ), against ischaemia-reperfusion (I/R) injury in the testis tissue of rats. Twenty-seven male Wistar albino rats were randomly divided into three equal groups as follows: Group I, sham group; Group II, torsion group; and Group III, torsion + thymoquinone group. The ischaemia period was 2 h, and orchiectomy was performed after 30 min of detorsion. Testis tissue sections were analysed with the terminal transferase mediated dUTP-nick end labelling (TUNEL) assay to determine in situ apoptotic DNA fragmentation. Additionally, Caspase 3 and Bax proteins were analysed immunohistochemically. The superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) activity levels in the testis tissue were also measured. The superoxide dismutase activity and malondialdehyde levels in the torsion group were significantly higher than those of the sham group (P < 0.05). Thymoquinone administration significantly reduced these levels. Torsion significantly increased active-Caspase 3 and Bax expression, which was decreased by thymoquinone. The apoptotic index of the torsion group was significantly higher than that of the control group. However, thymoquinone significantly reduced the apoptotic index (P < 0.05). Our results indicate that thymoquinone plays a protective role in oxidative stress induced ischaemia-reperfusion in the testis tissue of rats.
authors.affiliation: Department of Emergency Medicine, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Ayan
authors.affiliation: Department of Anatomy, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey.
authors.forename: U
authors.initials: U
authors.lastname: Tas
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Katip Celebi University, İzmir, Turkey.
authors.forename: E
authors.initials: E
authors.lastname: Sogut
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Yildirim Beyazit University, Ankara, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Caylı
authors.affiliation: Department of Emergency Medicine, Faculty of Medicine, Harran University, Urfa, Turkey.
authors.forename: H
authors.initials: H
authors.lastname: Kaya
authors.affiliation: Department of Emergency Medicine, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Esen
authors.affiliation: Department of Urology, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey.
authors.forename: F
authors.initials: F
authors.lastname: Erdemir
authors.affiliation: Department of Anatomy, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Uysal
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D016227:Benzoquinones / Q000494:pharmacology*; D053148:Caspase 3 / Q000187:drug effects / Q000378:metabolism; D002374:Catalase / Q000187:drug effects / Q000378:metabolism; D053938:DNA Fragmentation / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000187:drug effects / Q000378:metabolism; D020287:In Situ Nick-End Labeling; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009919:Orchiectomy; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism*; D013086:Spermatic Cord Torsion / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000187:drug effects / Q000378:metabolism; D013737:Testis / Q000187:drug effects* / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000187:drug effects / Q000378:metabolism
chemical_list: C494828:Bax protein, rat; D016227:Benzoquinones; D051028:bcl-2-Associated X Protein; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; C505410:Casp3 protein, rat; D053148:Caspase 3; C003466:thymoquinone
keywords: Ischaemia-reperfusion injury; oxidative stress; testis; thymoquinone