title: Anti-inflammatory and protective investigations on the effects of Theranekron® "an alcoholic extract of the Tarantula cubensis" on wound healing of peritoneal in the rat: an in vivo comparative study.
abstract: BACKGROUND
  The present study sought to investigate the effects of Tarantula cubensis extract (TC; Theranekron®) on the histopathological scores of peritoneal wound healing after laparotomy in the rats.
  
  
  METHODS
  This study was designed to investigate the effects of Theranekron on the peritoneal wound healing after wound creation, on days9, 14, 19, 24 and 29 post-injury in rats. Twenty-four mature Wister-albino male rats were randomly divided into two groups. In the experimental group, TC was repeatedly injected subcutaneously (SC) over the lesion 9, 14,19 and 24 days after laparotomy, whereas the control group received only normal saline by subcutaneous injection and then the animal groups were euthanized9, 14, 19, 24, and 29 days after wounding respectively by intravenous injections of pentobarbital (50 mg/kg). Finally, assessment of the peritoneal wound healing between the groups was carried out by histopathologic data and statistical tests as Mann-Whitney U, Wilcoxon W and Z RESULTS: Histopathological examination indicated significant improvement in angiogenesis, re-epithelialization and less inflammatory response in comparison to control and also, revealed matured, compact and parallel deposition of collagen fibrils on day 29. So, at long term, treatment reduced the inflammation and increased the quality and rate of wound re- epithelialization compared to controls(P < 0.05). Furthermore, excluding the control group, rats exhibited the most pronounced effect on wound closure, with the statistically significant improvement in wound healing being seen at post-operative day 29. Moreover, collagen content on days 24 and 29 in the test group was found to be higher than in the healthy group. To warp up, treated groups had a significant increase in peritoneal wound healing area compared to the control group on all days (P < 0.05).
  
  
  CONCLUSIONS
  Our results suggested that Theranekron have delivered a novel therapeutic route for wound treatment in clinical practice.
  The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/2958770714954315 .
authors.affiliation: Department of Clinical Science, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran. fahashemi@gmail.com.
authors.forename: Farajollah
authors.initials: F
authors.lastname: Adib-Hashemi
authors.affiliation: Graduated, Faculty of Veterinary Medicine, Garmsar Branch, Islamic Azad University, Garmsar, Iran. ffarahmand@yahoo.com.
authors.forename: Farshad
authors.initials: F
authors.lastname: Farahmand
authors.affiliation: Orthopedic Surgeon, Department of Surgery, Imam Reza hospital, Tehran, Iran. shamimfh@yahoo.com.
authors.forename: Shamim Fattah
authors.initials: SF
authors.lastname: Hesari
authors.affiliation: Department of Urology, Imam Reza Hospital, Tehran, Iran. bijanrkh@gmail.com.
authors.forename: Bijan
authors.initials: B
authors.lastname: Rezakhaniha
authors.affiliation: Department of Orthopedic and Trauma Surgery, AJA University of Medical Sciences, Tehran, Iran. ehsanf@yahoo.com.
authors.forename: Ehsan
authors.initials: E
authors.lastname: Fallah
authors.affiliation: Departmen of Legal Medicine, AJA University of Medical Sciences, Tehran, Iran. amirff@yahoo.com.
authors.forename: Amir Farshid
authors.initials: AF
authors.lastname: Fayyaz
authors.affiliation: Department of Pathology, Imam Reza Hospital, AJA University of Medical Sciences, Tehran, Iran. mdadpay@yahoo.com.
authors.forename: Masoomeh
authors.initials: M
authors.lastname: Dadpay
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000494:pharmacology; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000494:pharmacology*; D003094:Collagen / Q000378:metabolism; D004195:Disease Models, Animal; D004334:Drug Administration Schedule; D007279:Injections, Subcutaneous; D008297:Male; D018919:Neovascularization, Physiologic / Q000187:drug effects; D010537:Peritoneum / Q000098:blood supply / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D020011:Protective Agents / Q000008:administration & dosage / Q000494:pharmacology*; D017208:Rats, Wistar; D062326:Re-Epithelialization / Q000187:drug effects; D013111:Spider Venoms / Q000008:administration & dosage / Q000494:pharmacology*; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology
chemical_list: D043925:Angiogenesis Inducing Agents; D000893:Anti-Inflammatory Agents; D020011:Protective Agents; D013111:Spider Venoms; C000588659:theranekron; D003094:Collagen