title: Myoglobin oxygenation and autoxidation in three reptilian species.
abstract: Differences between species in the oxygen (O2) affinity (P50) of myoglobin (Mb) may serve to fine tune O2 supply to cardiac and skeletal muscle in ectotherms. In support of this view, it has been shown that fish Mb O2 affinities differ between species when measured at the same temperature, but are in fact similar when adjusted for in vivo muscle temperatures, most likely to maintain intracellular O2 delivery in species adapted to different environments. It is unknown whether similar adaptations exist in the O2 affinity of Mb from reptiles, despite this group of ectothermic vertebrates displaying great variation in the tolerance to both temperature and hypoxia. In this study, we have purified Mb from muscle tissues of three reptilian species (turtle, tortoise and alligator) with different lifestyles. We have measured O2 binding characteristics and autoxidation rates of the three Mbs and measured the effects of temperature, lactate and blocking of reactive thiols on the O2 affinity of turtle Mb. Our data show that, at a constant temperature, reptilian Mbs have similar O2 affinities that are lower than those of mammalian Mbs, which may optimize intracellular O2 transport at lower body temperatures. Reptilian Mbs have lower autoxidation rates than both mammalian and fish Mbs, which may be beneficial during oxidative stress. Furthermore, the O2 affinity of turtle Mb is without allosteric control and independent of either lactate or thiol covalent modification. This study reveals some common adaptive patterns in the temperature-dependent regulation of Mb oxygenation in vertebrates.
authors.affiliation: Zoophysiology, Department of Bioscience, Aarhus University, C.F. Møllers allé 3, Bldg. 1131, DK-8000 Aarhus C, Denmark. Electronic address: signe.helbo@biology.au.dk.
authors.forename: Signe
authors.initials: S
authors.lastname: Helbo
authors.affiliation: Zoophysiology, Department of Bioscience, Aarhus University, C.F. Møllers allé 3, Bldg. 1131, DK-8000 Aarhus C, Denmark.
authors.forename: Amanda G
authors.initials: AG
authors.lastname: Bundgaard
authors.affiliation: Zoophysiology, Department of Bioscience, Aarhus University, C.F. Møllers allé 3, Bldg. 1131, DK-8000 Aarhus C, Denmark.
authors.forename: Angela
authors.initials: A
authors.lastname: Fago
mesh_terms: D000818:Animals; D009211:Myoglobin / Q000378:metabolism*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D012104:Reptiles / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D009211:Myoglobin; D010100:Oxygen
keywords: Alligator; Allosteric effect; Autoxidation; Comparative; Oxygen affinity; Reptiles; S-nitrosation; Tortoise; Turtle; Vertebrates