title: The clinicopathological significance of miR-1307 in chemotherapy resistant epithelial ovarian cancer.
abstract: BACKGROUND
  We aimed to examine the expression of miR-1307 in chemosensitive and chemoresistant epithelial ovarian cancer tissues and cell lines and to analyze the clinicopathological significance of miR-1307 in ovarian cancer.
  
  
  METHODS
  MicroRNA microarray was used to screen differentially expressed microRNAs between the chemosensitive and chemoresistant epithelial ovarian cancer tissues. RT-PCR was used to validate the candidate microRNA. The potential target genes and their enriched biological pathways of microRNA were also analyzed. Dual Luciferase Reporter Gene Assay was conducted to validate the regulation of miRNA-1307 on the 3'-UTR of DAPK3.
  
  
  RESULTS
  miRNA-1307 was up-regulated in the chemoresistant epithelial ovarian cancer tissues compared to the chemosensitive counterparts. The up-regulation of miRNA-1307 was not associated with menopause, tumor differentiation state, clinical stage, and lymph node metastasis of ovarian cancer. Gene ontology analysis of miR-1307 candidate target genes indicated that miR-1307 candidate target genes were enriched in the processes of cell proliferation and differentiation, nucleotide synthesis and metabolism, and lymphocytes activation.
  
  
  CONCLUSIONS
  Our results suggest that miRNA-1307 may play a role in the development of chemoresistance in ovarian cancer.
authors.affiliation: Department of Gynecology and Obstetrics, Shengjing Hospital of China Medical University, 36 Sanhao Street, Shenyang, Liaoning, 110004, P.R. China. zyy03300821@163.com.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Gynecology and Obstetrics, Shengjing Hospital of China Medical University, 36 Sanhao Street, Shenyang, Liaoning, 110004, P.R. China. wm21st@hotmail.com.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Gynecology and Obstetrics, Shengjing Hospital of China Medical University, 36 Sanhao Street, Shenyang, Liaoning, 110004, P.R. China. jianleiwu365@163.com.
authors.forename: Jianlei
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Gynecology and Obstetrics, Shengjing Hospital of China Medical University, 36 Sanhao Street, Shenyang, Liaoning, 110004, P.R. China. 840921855@qq.com.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Jie
authors.affiliation: Department of Gynecology and Obstetrics, Shengjing Hospital of China Medical University, 36 Sanhao Street, Shenyang, Liaoning, 110004, P.R. China. c.shuang-0519@163.com.
authors.forename: Shuang
authors.initials: S
authors.lastname: Chang
authors.affiliation: Department of Gynecology and Obstetrics, Shengjing Hospital of China Medical University, 36 Sanhao Street, Shenyang, Liaoning, 110004, P.R. China. ssttkaoyan@163.com.
authors.forename: Ting
authors.initials: T
authors.lastname: Shuang
mesh_terms: D000328:Adult; D000368:Aged; D000077216:Carcinoma, Ovarian Epithelial; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D064550:Death-Associated Protein Kinases / Q000096:biosynthesis* / Q000235:genetics; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008207:Lymphatic Metastasis; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D009375:Neoplasms, Glandular and Epithelial / Q000188:drug therapy* / Q000235:genetics* / Q000473:pathology; D010051:Ovarian Neoplasms / Q000188:drug therapy* / Q000235:genetics* / Q000473:pathology
chemical_list: C000610094:MIRN1307 microRNA, human; D035683:MicroRNAs; C579101:DAPK3 protein, human; D064550:Death-Associated Protein Kinases