title: Static magnetic field attenuates lipopolysaccharide-induced inflammation in pulp cells by affecting cell membrane stability.
abstract: One of the causes of dental pulpitis is lipopolysaccharide- (LPS-) induced inflammatory response. Following pulp tissue inflammation, odontoblasts, dental pulp cells (DPCs), and dental pulp stem cells (DPSCs) will activate and repair damaged tissue to maintain homeostasis. However, when LPS infection is too serious, dental repair is impossible and disease may progress to irreversible pulpitis. Therefore, the aim of this study was to examine whether static magnetic field (SMF) can attenuate inflammatory response of dental pulp cells challenged with LPS. In methodology, dental pulp cells were isolated from extracted teeth. The population of DPSCs in the cultured DPCs was identified by phenotypes and multilineage differentiation. The effects of 0.4 T SMF on DPCs were observed through MTT assay and fluorescent anisotropy assay. Our results showed that the SMF exposure had no effect on surface markers or multilineage differentiation capability. However, SMF exposure increases cell viability by 15%. In addition, SMF increased cell membrane rigidity which is directly related to higher fluorescent anisotropy. In the LPS-challenged condition, DPCs treated with SMF demonstrated a higher tolerance to LPS-induced inflammatory response when compared to untreated controls. According to these results, we suggest that 0.4 T SMF attenuates LPS-induced inflammatory response to DPCs by changing cell membrane stability.
authors.affiliation: School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 11031, Taiwan ; Department of Dentistry, Wan Fang Hospital, Taipei 11696, Taiwan.
authors.forename: Sung-Chih
authors.identifier: 0000-0003-1627-1844
authors.initials: SC
authors.lastname: Hsieh
authors.affiliation: Division of Allergy and Immunology, Department of Internal Medicine, Cathay General Hospital, Taipei 10630, Taiwan.
authors.forename: Jeng-Ting
authors.initials: JT
authors.lastname: Tsao
authors.affiliation: School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 11031, Taiwan.
authors.forename: Wei-Zhen
authors.identifier: 0000-0002-3552-6892
authors.initials: WZ
authors.lastname: Lew
authors.affiliation: School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 11031, Taiwan.
authors.forename: Ya-Hui
authors.identifier: 0000-0003-1234-4866
authors.initials: YH
authors.lastname: Chan
authors.affiliation: Department of Microbiology and Immunology, Taipei Medical University, Taipei 11031, Taiwan.
authors.forename: Lin-Wen
authors.initials: LW
authors.lastname: Lee
authors.affiliation: School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 11031, Taiwan.
authors.forename: Che-Tong
authors.initials: CT
authors.lastname: Lin
authors.affiliation: School of Oral Hygiene, College of Oral Medicine, Taipei Medical University, 250 Wu-Hsing Street, Taipei 11031, Taiwan.
authors.forename: Yung-Kai
authors.identifier: 0000-0002-4467-0300
authors.initials: YK
authors.lastname: Huang
authors.affiliation: School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 11031, Taiwan ; Graduate Institute of Biomedical Materials and Tissue Engineering, College of Dental Medicine, Taipei Medical University, 250 Wu-Hsing Street, Taipei 11031, Taiwan.
authors.forename: Haw-Ming
authors.identifier: 0000-0003-4184-3744
authors.initials: HM
authors.lastname: Huang
mesh_terms: D002462:Cell Membrane / Q000378:metabolism*; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D003782:Dental Pulp / Q000187:drug effects / Q000473:pathology*; D005434:Flow Cytometry; D005454:Fluorescence Polarization; D006801:Humans; D007249:Inflammation / Q000473:pathology*; D008070:Lipopolysaccharides; D060526:Magnetic Fields; D013194:Staining and Labeling
chemical_list: D008070:Lipopolysaccharides