title: Osteogenesis around CaP-coated titanium implants visualized using 3D histology and micro-computed tomography.
abstract: Calcium phosphate (CaP) coatings can enhance the performance of bone implants in compromised conditions, such as osteoporosis. Therefore, this study compared non-coated vs. CaP-coated (n = 8) titanium implants in osteoporotic ovariectomized (OVX) rats. Bone volume (BV) was assessed using micro-computer tomography (micro-CT) and three-dimensional (3D) histology, in three zones from the implant surface. Bone remodeling was assessed using fluorochrome labels and osteoclast staining. Micro-CT and 3D histology showed a BV reduction in OVX animals, of respectively 22.4 and 10.5%. BV was significantly increased inside all zones around CaP coatings, especially in the inner zone of the OVX animals. Fluorochrome labels were predominantly seen when the coating was applied. Osteoclasts were mainly found in the area remote from the surface of non-coated implants in control animals. For the coated implants, osteoclasts were distributed evenly, and present in direct vicinity of the surface. In conclusion, 3D histology is a suitable technique to obtain data and insight into bone architecture around implants at relatively high resolution. Bone formation was significantly reduced in osteoporotic animals. CaP coatings resulted in a higher BV directly around implants installed in osteoporotic animals, enhanced turnover, and a shift of remodeling activity toward the implant surface.
authors.affiliation: Department of Biomaterials, Radboud University Medical Center, 6500 HB Nijmegen, The Netherlands.
authors.forename: Vincent M J I
authors.initials: VM
authors.lastname: Cuijpers
authors.affiliation: Department of Biomaterials, Radboud University Medical Center, 6500 HB Nijmegen, The Netherlands.|Department of Periodontics and Community Dentistry, College of Dentistry, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Hamdan S
authors.initials: HS
authors.lastname: Alghamdi
authors.affiliation: Department of Biomaterials, Radboud University Medical Center, 6500 HB Nijmegen, The Netherlands.
authors.forename: Natasja W M
authors.initials: NW
authors.lastname: Van Dijk
authors.affiliation: Faculty of Materials Science and Engineering, Warsaw University of Technology, Warszawa, 02-507, Poland.
authors.forename: Jakub
authors.initials: J
authors.lastname: Jaroszewicz
authors.affiliation: Department of Biomaterials, Radboud University Medical Center, 6500 HB Nijmegen, The Netherlands.
authors.forename: X Frank
authors.initials: XF
authors.lastname: Walboomers
authors.affiliation: Department of Biomaterials, Radboud University Medical Center, 6500 HB Nijmegen, The Netherlands.
authors.forename: John A
authors.initials: JA
authors.lastname: Jansen
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D002130:Calcium Phosphates / Q000494:pharmacology*; D005260:Female; D005269:Femur / Q000000981:diagnostic imaging / Q000187:drug effects; D021621:Imaging, Three-Dimensional; D007527:Isoenzymes / Q000378:metabolism; D008856:Microscopy, Fluorescence; D010012:Osteogenesis / Q000187:drug effects*; D019736:Prostheses and Implants; D017208:Rats, Wistar; D040521:Subcutaneous Tissue / Q000187:drug effects; D000071681:Tartrate-Resistant Acid Phosphatase; D014025:Titanium / Q000494:pharmacology*; D055114:X-Ray Microtomography
chemical_list: D002130:Calcium Phosphates; D007527:Isoenzymes; C020243:calcium phosphate; D014025:Titanium; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase
keywords: 3D histology; biomaterials; calcium phosphate scaffolds; micro computed tomography; osteoporosis