title: Leishmania (infantum) chagasi in canine urinary sediment.
abstract: Canine visceral leishmaniasis (CVL) is difficult to diagnosis, mainly due to the presence of asymptomatic animals, the diversity of clinical symptoms and the difficulty in obtaining diagnostic evidence of high sensitivity and specificity. The purpose of this study was to diagnose CVL in urinary sediment of 70 dogs of different breeds, sexes and ages from the veterinary hospital of the Federal University of Piauí and Zoonosis Control Center of Teresina, Brazil. The serological tests were TR DPP® for CVL and enzyme-linked immunosorbent assay (ELISA) for CVL, parasitological exams of bone marrow and lymph nodes and urine sediment cultures. Leishmania was detected in the bone marrow and/or lymph node of 61.0% of the animals (43/70), and urine sediment culture was positive in 9.30% (4/43) of these animals. In the serological exams, 70.0% (49/70) were reactive using the DPP and 78.2% (55/70) were reactive using ELISA. The goal of this study was to diagnose the presence of L. (infantum) chagasi in a culture of urinary sediment.
authors.affiliation: Department of Veterinary Science and Surgery, Centro de Ciências Agrárias, Universidade Federal do Piauí, Teresina, PI, Brazil.
authors.forename: Ivete Lopes
authors.initials: IL
authors.lastname: de Mendonça
authors.affiliation: Programa de Pós-graduação em Ciência Animal, Universidade Federal do Piauí, Teresina, PI, Brazil.
authors.forename: Joilson Ferreira
authors.initials: JF
authors.lastname: Batista
authors.affiliation: Departmento de Medicina Veterinária, Universidade Federal Rural de Pernambuco, Recife, PE, Brazil.
authors.forename: Leucio Camara
authors.initials: LC
authors.lastname: Alves
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000175:diagnosis* / Q000469:parasitology* / Q000652:urine; D004285:Dogs; D005260:Female; D018314:Leishmania infantum / Q000302:isolation & purification*; D007898:Leishmaniasis, Visceral / Q000175:diagnosis / Q000652:urine / Q000662:veterinary*; D008297:Male; D014556:Urine / Q000469:parasitology*