title: GRY79 encoding a putative metallo-β-lactamase-trihelix chimera is involved in chloroplast development at early seedling stage of rice.
abstract: The green - revertible yellow79 mutant resulting from a single-base mutation suggested that the GRY79 gene encoding a putative metallo-β-lactamase-trihelix chimera is involved in chloroplast development at early seedling stage of rice. Functional studies of metallo-β-lactamases and trihelix transcription factors in higher plants remain very sparse. In this study, we isolated the green-revertible yellow79 (gry79) mutant in rice. The mutant developed yellow-green leaves before the three-leaf stage but recovered to normal green at the sixth-leaf stage. Meanwhile, the mutant exhibited reduced level of chlorophylls and arrested development of chloroplasts in the yellow leaves. Genetic analysis suggested that the mutant phenotype was controlled by a single recessive nuclear gene on rice chromosome 2. Map-based cloning revealed that the candidate gene was Os02g33610 encoding a putative metallo-β-lactamase-trihelix chimera. In the gry79 mutant, a single-base mutation occurred in coding region of the gene, resulting in an amino acid change in the encoded protein. Furthermore, the mutant phenotype was rescued by transformation with the wild-type gene. Therefore, we have confirmed that the gry79 mutant phenotype resulted from a single-base mutation in GRY79 (Os02g33610) gene, suggesting that the gene encoding a putative metallo-β-lactamase-trihelix chimera is involved in chloroplast development at early seedling stage of rice. In addition, we considered that the gry79 mutant gene could be applicable as a leaf-color marker gene for efficient identification and elimination of false hybrids in commercial hybrid rice production.
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Wan
authors.forename: Chunmei
authors.initials: C
authors.lastname: Li
authors.forename: Xiaozhi
authors.initials: X
authors.lastname: Ma
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Changhui
authors.initials: C
authors.lastname: Sun
authors.forename: Rui
authors.initials: R
authors.lastname: Huang
authors.forename: Ping
authors.initials: P
authors.lastname: Zhong
authors.forename: Zhiyan
authors.initials: Z
authors.lastname: Gao
authors.forename: Dan
authors.initials: D
authors.lastname: Chen
authors.forename: Zhengjun
authors.initials: Z
authors.lastname: Xu
authors.forename: Jianqing
authors.initials: J
authors.lastname: Zhu
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Gao
authors.forename: Pingrong
authors.initials: P
authors.lastname: Wang
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Deng
mesh_terms: D019943:Amino Acid Substitution / Q000235:genetics / Q000502:physiology; D002736:Chloroplasts / Q000502:physiology*; D017343:Genes, Plant / Q000235:genetics / Q000502:physiology*; D050506:Mutant Chimeric Proteins / Q000235:genetics* / Q000502:physiology; D012275:Oryza / Q000235:genetics / Q000254:growth & development*; D036226:Seedlings / Q000254:growth & development*; D013347:Subcellular Fractions / Q000737:chemistry; D014157:Transcription Factors / Q000235:genetics* / Q000502:physiology; D001618:beta-Lactamases / Q000235:genetics* / Q000502:physiology
chemical_list: D050506:Mutant Chimeric Proteins; D014157:Transcription Factors; D001618:beta-Lactamases