title: Isolation and Characterization of Dammarane-Type Saponins from Gynostemma pentaphyllum and Their Inhibitory Effects on IL-6-Induced STAT3 Activation.
abstract: Five new dammarane-type saponins, gypenosides GD1-GD5 (1-5), along with six known saponins (6-11), were isolated from the aerial parts of Gynostemma pentaphyllum using various chromatographic methods. Their structures were elucidated by a combination of spectroscopic and spectrometric data, including 1D and 2D NMR and HRESIMS. All isolates were tested for their inhibitory effects on IL-6-induced STAT3 promoter activity in Hep3B cells. Compounds 1, 9, and 11 displayed potent inhibitory effects, with IC50 values ranging from 0.27 to 0.59 μM.
authors.affiliation: †College of Pharmacy, Chungbuk National University, Cheongju 362-763, Republic of Korea.|‡Eco-friendly Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup-si 580-185, Republic of Korea.
authors.forename: Chul
authors.initials: C
authors.lastname: Lee
authors.affiliation: †College of Pharmacy, Chungbuk National University, Cheongju 362-763, Republic of Korea.
authors.forename: Jin Woo
authors.initials: JW
authors.lastname: Lee
authors.affiliation: †College of Pharmacy, Chungbuk National University, Cheongju 362-763, Republic of Korea.
authors.forename: Qinghao
authors.initials: Q
authors.lastname: Jin
authors.affiliation: †College of Pharmacy, Chungbuk National University, Cheongju 362-763, Republic of Korea.
authors.forename: Hari
authors.initials: H
authors.lastname: Jang
authors.affiliation: ‡Eco-friendly Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup-si 580-185, Republic of Korea.
authors.forename: Hyun-Jae
authors.initials: HJ
authors.lastname: Jang
authors.affiliation: ‡Eco-friendly Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup-si 580-185, Republic of Korea.
authors.forename: Mun-Chual
authors.initials: MC
authors.lastname: Rho
authors.affiliation: †College of Pharmacy, Chungbuk National University, Cheongju 362-763, Republic of Korea.
authors.forename: Myung Koo
authors.initials: MK
authors.lastname: Lee
authors.affiliation: †College of Pharmacy, Chungbuk National University, Cheongju 362-763, Republic of Korea.
authors.forename: Chong Kil
authors.initials: CK
authors.lastname: Lee
authors.affiliation: †College of Pharmacy, Chungbuk National University, Cheongju 362-763, Republic of Korea.
authors.forename: Mi Kyeong
authors.initials: MK
authors.lastname: Lee
authors.affiliation: †College of Pharmacy, Chungbuk National University, Cheongju 362-763, Republic of Korea.
authors.forename: Bang Yeon
authors.initials: BY
authors.lastname: Hwang
mesh_terms: D029774:Gynostemma / Q000737:chemistry*; D020128:Inhibitory Concentration 50; D015850:Interleukin-6 / Q000494:pharmacology*; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D035261:Plant Components, Aerial / Q000737:chemistry; D056910:Republic of Korea; D050796:STAT3 Transcription Factor / Q000037:antagonists & inhibitors*; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000097669:Dammaranes
chemical_list: D015850:Interleukin-6; D050796:STAT3 Transcription Factor; D012503:Saponins; D014315:Triterpenes; D000097669:Dammaranes