title: Effect of NF- κ B inhibitor PDTC on VEGF and endostatin expression of mice with Lewis lung cancer.
abstract: OBJECTIVE
  To investigate the effects of NF- κ B inhibitor pyrrolidine dithiocarbamate hydrochloride (PDTC) on vascular endothelial growth factor (VEGF) and endostatin expression in mice with Lewis lung cance; and its mechanism.
  
  
  METHODS
  Mice survival rate and anti-tumor effects were observed in different concentrations of NF- κ B inhibitor PDTC after the Lewis lung cancer mice model was established. VEGF and endostatin expressions were detected by immunohistochemical assay.
  
  
  RESULTS
  Lewis lung cancer was be inhibited by 0.5 mg/kg, 1.5 mg/kg and 3.0 mg/kg of NF- κ B inhibitor PDTC (P<0.05). Microvessel density (MVD) in 0.5 mg/kg, 1.5 mg/kg and 3.0 mg/kg NF- κ B inhibitor PDTC groups were significantly lower than the control group (P<0.05). Immunohistochemical assay results showed that VEGF and endostatin expressions in the 0.5 mg/kg, 1.5 mg/kg and 3.0 mg/kg NF-κ B inhibitor PDTC groups were significantly lower than the control group (P<0.05). Western blot results also showed that NF- κ B inhibitor PDTC could inhibit VEGF and endostatin expressions in tumor tissues.
  
  
  CONCLUSIONS
  NF- κ B inhibitor PDTC can inhibit tumor formation and reduce tumor angiogenesis in mice with Lewis lung cancer; and its mechanism maybe associated to VEGF and endostatin down-regulation.
authors.affiliation: Oncology Department, First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116000, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Gao
authors.affiliation: Oncology Department, First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116000, China. Electronic address: Gaoyajie@yahoo.com.cn.
authors.forename: Ya-Jie
authors.initials: YJ
authors.lastname: Gao
authors.affiliation: Oncology Department, First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116000, China. Electronic address: gaopinggaoping2008@163.com.
authors.forename: Hong-Lu
authors.initials: HL
authors.lastname: Liang
keywords: Endostatin; Mice with Lewis lung cancer; PDTC; Pyrrolidine dithiocarbamate hydrochloride; Vascular density; Vascular endothelial growth factor