title: Pre-treatment with puerarin affects pharmacokinetics of warfarin, but not clopidogrel, in experimental rats.
abstract: The present study was designed to determine the effects of puerarin pre-treatment on the pharmacokinetics of the oral anticoagulant agent warfarin and the antiplatelet agent clopidogrel in rats. In the treatment group, rats was gavaged with warfarin or clopidogrel after repeated treatment with puerarin at intraperitoneal doses of 20, 60, or 200 mg·kg(-1) for 7 days, while rats in the control group were administrated only with the same dose warfarin or clopidogrel. Plasma samples were obtained at the prescribed times and analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS). The results showed that rats treated with puerarin at all the test doses of 20, 60 and 200 mg·kg(-1) were found to affect the pharmacokinetics of warfarin, but not clopidogrel, suggesting a potential herb-drug interaction between puerarin and warfarin.
authors.affiliation: Qilu hospital of Shandong University, Jinan 250012, China.
authors.forename: An-Chang
authors.initials: AC
authors.lastname: Liu
authors.affiliation: Qilu hospital of Shandong University, Jinan 250012, China.
authors.forename: Li-Xia
authors.initials: LX
authors.lastname: Zhao
authors.affiliation: Jinan Central Hospital Affiliated to Shandong University, Jinan 250011, China.
authors.forename: Shu-Wen
authors.initials: SW
authors.lastname: Yu
authors.affiliation: School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China. Electronic address: louhongxiang@sdu.edu.cn.
authors.forename: Hong-Xiang
authors.initials: HX
authors.lastname: Lou
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000493:pharmacokinetics*; D002853:Chromatography, Liquid; D000077144:Clopidogrel; D004334:Drug Administration Schedule; D041743:Herb-Drug Interactions; D007274:Injections, Intraperitoneal; D007529:Isoflavones / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D010975:Platelet Aggregation Inhibitors / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D053719:Tandem Mass Spectrometry; D013988:Ticlopidine / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D014665:Vasodilator Agents / Q000008:administration & dosage / Q000494:pharmacology*; D014859:Warfarin / Q000493:pharmacokinetics*
chemical_list: D000925:Anticoagulants; D007529:Isoflavones; D010975:Platelet Aggregation Inhibitors; D014665:Vasodilator Agents; D014859:Warfarin; D000077144:Clopidogrel; D013988:Ticlopidine; C033607:puerarin
keywords: Clopidogrel; Drug interaction; Pharmacokinetics; Puerarin; Warfarin