title: Salvianolic acid B improves bone marrow-derived mesenchymal stem cell differentiation into alveolar epithelial cells type I via Wnt signaling.
abstract: Acute lung injury (ALI) is among the most common causes of mortality in intensive care units. Previous studies have suggested that bone marrow-derived mesenchymal stem cells (BMSCs) may attenuate pulmonary edema. In addition, alveolar epithelial cells type I (ATI) are involved in reducing the alveolar edema in response to ALI. However, the mechanism involved in improving the efficiency of differentiation of MSCs into ATI remains to be elucidated. In the present study, the effect of salvianolic acid B (Sal B) on the differentiation of BMSCs into ATI and the activities of the Wnt signaling pathways were investigated. The BMSCs were supplemented with conditioned medium (CM). The groups were as follows: i) CM group: BMSCs were supplemented with CM; ii) lithium chloride (LiCl) group: BMSCs were supplemented with CM and 5 mM LiCl; iii) Sal B group: BMSCs were supplemented with CM and 10 mM Sal B. The samples were collected and assessed on days 7 and 14. It was revealed that aquaporin (AQP)-5 and T1α were expressed in BMSCs, and induction with LiCl or Sal B increased the expression of AQP-5 and T1α. Furthermore, the Wnt-1 and Wnt-3a signaling pathways were activated during the differentiation of BMSCs into ATI. In conclusion, it was suggested that the promotive effects of Sal B on the differentiation of BMSCs into ATI occurred through the activation of Wnt signaling pathways.
authors.affiliation: Department of Anesthesiology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: Gao
authors.affiliation: Department of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, Liaoning 116600, P.R. China.
authors.forename: Jingxian
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Anesthesiology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Xi
authors.initials: X
authors.lastname: Gao
authors.affiliation: Department of Anesthesiology, First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China.
authors.forename: Dan
authors.initials: D
authors.lastname: Xu
authors.affiliation: Department of Anesthesiology, First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China.
authors.forename: Dongge
authors.initials: D
authors.lastname: Niu
authors.affiliation: Department of Anesthesiology, First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China.
authors.forename: Jinglin
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Anesthesiology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Qingping
authors.initials: Q
authors.lastname: Wen
mesh_terms: D000818:Animals; D001572:Benzofurans / Q000302:isolation & purification / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D011650:Pulmonary Alveoli / Q000166:cytology*; D017207:Rats, Sprague-Dawley; D020545:Respiratory Mucosa / Q000166:cytology*; D027544:Salvia / Q000737:chemistry; D051153:Wnt Proteins / Q000378:metabolism; D060449:Wnt Signaling Pathway / Q000187:drug effects*; D051176:beta Catenin / Q000378:metabolism; D053630:Depsides
chemical_list: D001572:Benzofurans; D017077:Culture Media, Conditioned; D004365:Drugs, Chinese Herbal; D051153:Wnt Proteins; D051176:beta Catenin; C076944:salvianolic acid B; D053630:Depsides