title: Evaluation of Kato-Katz and spontaneous sedimentation methods for the diagnosis of platynosomiasis in Neotropical primates.
abstract: Platynosomiasis is a parasitic infection reported in non-human primates, including marmosets, and is frequently difficult to diagnose. In this study, the Kato-Katz method and the spontaneous sedimentation method were evaluated for their usefulness in identifying Platynosomum eggs in fecal samples from Callithrix penicillata that naturally harbor Platynosomum illiciens. Spontaneous sedimentation allowed the diagnosis of 41.7% (5/12) and 66.7% (8/12) of infected marmosets from one and three slides, respectively, prepared from the same fecal sample. The examination of a single Kato-Katz thick smear detected 83.3% (10/12) of infection cases. The analysis of feces on three different days increased the rate of diagnosis, since 75% (9/12) and 100% (12/12) of the primates with platynosomiasis were identified using serial spontaneous sedimentation (3 slides/day) and the Kato-Katz method, respectively. The mean number of Platynosomum eggs per gram of feces determined via the Kato-Katz method was 71.7 (8-240). The spontaneous sedimentation method when performed in series is acceptable for the diagnosis of platynosomiasis. However, the Kato-Katz method, which was here used for the first time to detect this infection, has a higher diagnostic sensitivity and the advantage that a quantitative analysis of the eggs released in the host feces is possible.
authors.affiliation: Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
authors.forename: Vitor Luís Tenório
authors.initials: VL
authors.lastname: Mati
authors.affiliation: Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
authors.forename: Hudson Alves
authors.initials: HA
authors.lastname: Pinto
authors.affiliation: Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
authors.forename: Alan Lane
authors.initials: AL
authors.lastname: de Melo
mesh_terms: D000818:Animals; D001938:Brazil; D002144:Callithrix / Q000469:parasitology*; D004012:Dicrocoeliidae; D005243:Feces / Q000469:parasitology*; D005260:Female; D008297:Male; D008992:Monkey Diseases / Q000175:diagnosis* / Q000469:parasitology*; D010274:Parasitology / Q000379:methods; D014201:Trematode Infections / Q000175:diagnosis / Q000662:veterinary*