title: Case study interpretation: Report from the ICCS Annual Meeting, Seattle, 2014.
abstract: The Case Study Interpretation (CSI) cases presented at the 2014 International Clinical Cytometry Society (ICCS) meeting in Seattle illustrate the utility of state-of-the art multiparameter flow cytometry in the diagnosis of hematolymphoid neoplasms. Download the listmode files (Supporting Information) and test your analysis skills before reading the case reports, keeping in mind the following questions. How many separate abnormal mature B-cell populations can you identify, and how many of these represent different subtypes of B-cell neoplasm? How many separate abnormal mature T-cell populations can you identify, and do these represent different subtypes of T-cell neoplasm or phenotypic heterogeneity in one neoplasm? How many separate immature/blastic cell populations can you identify, and do they meet criteria for mixed phenotype leukemia? Is there a population of blasts that lacks T-cell, B-cell, and myeloid lineage defining antigens and if so, what entities should you consider and what additional antigens should you assess for?
authors.affiliation: UW Hematopathology Laboratory at SCCA, Seattle, Washington.
authors.forename: Jonathan R
authors.initials: JR
authors.lastname: Fromm
authors.affiliation: Mayo Clinic Rochester, Rochester, Minnesota.
authors.forename: Damian J
authors.initials: DJ
authors.lastname: Tagliente
authors.affiliation: Vanderbilt University Medical Center, Nashville, Tennessee.
authors.forename: Aaron C
authors.initials: AC
authors.lastname: Shaver
authors.affiliation: Department of Pathology, Roswell Park Cancer Institute, Buffalo, New York.
authors.forename: Vishala
authors.initials: V
authors.lastname: Neppalli
authors.affiliation: Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.
authors.forename: Fiona E
authors.initials: FE
authors.lastname: Craig
mesh_terms: D000369:Aged, 80 and over; D015703:Antigens, CD / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology / Q000473:pathology*; D003713:Dendritic Cells / Q000276:immunology / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D007938:Leukemia / Q000175:diagnosis / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology*
chemical_list: D015703:Antigens, CD
keywords: flow cytometry; leukemia; lymphoma; mixed phenotype; plasmacytoid dendritic