title: Seroprevalence and genotype of Toxoplasma gondii in pigs, dogs and cats from Guizhou province, Southwest China.
abstract: BACKGROUND
  Toxoplasma gondii is an obligate, intracellular protozoan that infects almost all warm-blooded animals, including humans, domesticated and wild animals. Recent studies of Toxoplasma gondii isolates from animals in different regions of China have shown a limited genetic diversity with the dominance of the ToxoDB PCR-RFLP genotype #9 named as "Chinese 1". However, there is not much published information regarding its prevalence in domestic animals from Guizhou province, a subtropical region in Southwest China. The objectives of this study were to determine seroprevalence and genetic diversity of T .gondii in pigs, dogs and cats in Guizhou province, Southwest China.
  
  
  RESULTS
  The anti-T. gondii IgG were detected in 70.0%(49/70) pigs, 20.56%(22/107) dogs and 63.16(12/19) cats. The anti-T. gondii IgM were found in 0.93%(1/107) dogs, 21.53%(4/19) cats, but not in pigs. In addition, the toxoplasma circulating antigen (CAG) were detected in 16.9%18/70)pigs, 13.1% (14/107) dogs and 10.5%(2/19) cats. The T. gondii DNA were detected in 31.5%(22/70) pigs, 3.7%(4/107) dogs and 52.63%(10/19) cats. Five T. gondii isolates were obtained(3 from pigs and 2 from cats). The genotype of these five isolates belonged to the predominant genotype "Chinese 1".
  
  
  CONCLUSIONS
  The high prevalence of T. gondii infection in pigs,cats and dogs indicated that the T. gondii infection is common in Guizhou province. Additionally, the T. gondii genotype "Chinese 1" was dominant in Southwest China.
authors.affiliation: Department of Immunology, Guiyang Medical College, Guiyang, 550001, China. lyn@gmc.edu.cn.
authors.forename: Yong-Nian
authors.initials: YN
authors.lastname: Li
authors.affiliation: Department of Immunology, Guiyang Medical College, Guiyang, 550001, China. 1628834450@qq.com.
authors.forename: XinWen
authors.initials: X
authors.lastname: Nie
authors.affiliation: Department of Laboratory, Guiyang Medical College, Guiyang, 550001, China. 674822868@qq.com.
authors.forename: Qun-Yi
authors.initials: QY
authors.lastname: Peng
authors.affiliation: Department of Immunology, Guiyang Medical College, Guiyang, 550001, China. 946678342@qq.com.
authors.forename: Xiao-Qiong
authors.initials: XQ
authors.lastname: Mu
authors.affiliation: Department of Clinical Laboratory, The Fourth Hospital of Guiyang, Guiyang, 550004, China. 807382208@qq.com.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Laboratory, Guiyang Medical College, Guiyang, 550001, China. 15660594@qq.com.
authors.forename: Meng-Yuan
authors.initials: MY
authors.lastname: Tian
authors.affiliation: Department of Clinical Laboratory, Baiyun Hospital Affiliated to Guiyang Medical College, Guiyang, 264000, China. 307932623@qq.com.
authors.forename: Shao-ju
authors.initials: SJ
authors.lastname: Min
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000453:epidemiology* / Q000469:parasitology; D002415:Cats; D002681:China / Q000453:epidemiology; D004283:Dog Diseases / Q000453:epidemiology* / Q000469:parasitology; D004285:Dogs; D005838:Genotype; D016036:Seroepidemiologic Studies; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000469:parasitology; D014122:Toxoplasma / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D014124:Toxoplasmosis, Animal / Q000453:epidemiology* / Q000469:parasitology