title: [Study on fluorescence labeling and determination of polypeptide (PC2~PC6) by high performance liquid chromatography].
abstract: This study was based on the thiol groups (-SH) of PC2~PC6, which could be reacted with the Monobromobimane (mBBr), in order to get polypeptide derivatives with fluorescent signal. A new method was developed for measuring the Polypeptides by high performance liquid chromatography with fluorescence detector, then the chromatographic conditions of HPLC was optimized; meawhile the reaction proportion of PCs and mBBr was identified by Trap-MS. The results showed that, the reaction proportion of PCs and mBBr was 1:1, the polypeptide derivatives had good stability; the five compounds separation was better, and the peak time focused on the 16.6~22.0 min; the linear correlation coefficient of PC2, PC3, PC4, PC5 and PC6 was >0.9991, and the limits of quantification were 0.3, 0.05, 0.3, 0.5 and 0.8 mg · L(-1) respectively, the recovery rate was 83.0%-102.0%; the method was reproducible, RSD<2%, this method for measuring the peptide compounds was rapid and accurate.
authors.forename: Jing-xi
authors.initials: JX
authors.lastname: Li
authors.forename: Li-jie
authors.initials: LJ
authors.lastname: Gao
authors.forename: Wei
authors.initials: W
authors.lastname: Cao
authors.forename: Li
authors.initials: L
authors.lastname: Zheng
authors.forename: Jun-hui
authors.initials: JH
authors.lastname: Chen
authors.forename: Xiu-li
authors.initials: XL
authors.lastname: Xu
authors.forename: Xiao-ru
authors.initials: XR
authors.lastname: Wang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005453:Fluorescence; D010455:Peptides / Q000737:chemistry*; D015203:Reproducibility of Results; D013438:Sulfhydryl Compounds
chemical_list: D010455:Peptides; D013438:Sulfhydryl Compounds