title: Baicalin promotes embryo adhesion and implantation by upregulating fucosyltransferase IV (FUT4) via Wnt/beta-catenin signaling pathway.
abstract: Glycosylation plays a significant role in determining the receptivity of the uterine endometrium to embryo. Fucosyltransferase IV (FUT4) is expressed stage-specifically in the uterine endometrium of mammalians, and considered as a marker of the endometrial receptivity. Baicalin, a monomer of flavonoids, is known to have functions in improving reproduction. However, the mechanism by which baicalin regulates the expression of FUT4 in embryo-endometrium adhesion remains unclear. Our results showed that baicalin significantly increased FUT4 mRNA and protein expression levels both in human endometrial cells and mouse endometrial tissue, and consistently elevated embryo adhesion rate during implantation in vitro and embryonic implantation competence in pregnant mouse. This study suggests that baicalin facilitates endometrial reproduction via elevating FUT4 expression through Wnt/β-catenin signaling pathway.
authors.affiliation: Department of Biochemistry and Molecular Biology, Dalian Medical University, Liaoning Provincial Core Lab of Glycobiology and Glycoengineering, Dalian, People's Republic of China.
authors.forename: Yu-Mei
authors.initials: YM
authors.lastname: Zhang
authors.affiliation: Department of Pathology and Pathophysiology, Medical School of Dalian University, Dalian, People's Republic of China.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry and Molecular Biology, Dalian Medical University, Liaoning Provincial Core Lab of Glycobiology and Glycoengineering, Dalian, People's Republic of China.
authors.forename: Ahmmed
authors.initials: A
authors.lastname: Bulbul
authors.affiliation: Department of Biochemistry and Molecular Biology, Dalian Medical University, Liaoning Provincial Core Lab of Glycobiology and Glycoengineering, Dalian, People's Republic of China.
authors.forename: Xiu
authors.initials: X
authors.lastname: Shan
authors.affiliation: Department of Dermatology, Northwestern University, Feinberg School of Medicine, Chicago, USA.
authors.forename: Xiao-Qi
authors.initials: XQ
authors.lastname: Wang
authors.affiliation: Department of Biochemistry and Molecular Biology, Dalian Medical University, Liaoning Provincial Core Lab of Glycobiology and Glycoengineering, Dalian, People's Republic of China. Electronic address: yanqiu63@126.com.
authors.forename: Qiu
authors.initials: Q
authors.lastname: Yan
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002460:Cell Line; D010064:Embryo Implantation / Q000187:drug effects*; D004622:Embryo, Mammalian / Q000166:cytology / Q000196:embryology* / Q000201:enzymology; D004717:Endometrium / Q000166:cytology / Q000201:enzymology*; D005260:Female; D005419:Flavonoids / Q000494:pharmacology*; D005647:Fucosyltransferases / Q000096:biosynthesis*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006801:Humans; D016256:Lewis X Antigen / Q000096:biosynthesis*; D051379:Mice; D011247:Pregnancy; D060449:Wnt Signaling Pathway / Q000187:drug effects*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D005419:Flavonoids; D016256:Lewis X Antigen; C038044:baicalin; C066693:FUT4 protein, human; D005647:Fucosyltransferases; C541914:Fut4 protein, mouse
keywords: Baicalin; Embryo; Endometrial epithelial cell; Fucosyltransferase IV; Implantation; β-Catenin