title: Spatial coordination of cell orientation directed by nanoribbon sheets.
abstract: Spatial coordination of cell orientation is of central importance in tissue/organ construction. In this study, we developed microfabricated poly(lactic-co-glycolic acid) (PLGA) nanoribbon sheets with unique structures, using spin-coating and micropatterning techniques, in order to generate a hierarchically assembled cellular structure consisting of murine skeletal myoblasts (C2C12). The nanoribbon sheets were composed of aligned PLGA nanoribbons in the center, and strips on four sides which take a role as bridges to connect and immobilize the aligned nanoribbons. Such unique structures facilitated the alignment of C2C12 cells into bilayer cell sheets, and cellular alignment was directed by the aligned direction of nanoribbons. The nanoribbon sheets also facilitated the construction of multilayer cell sheets with anisotropic (orthogonal) and isotropic (parallel) orientations. The enhanced expression of myogenic genes of C2C12 cells on the bilayer cell sheets demonstrated that the nanoribbons induced C2C12 cell differentiation into mature myoblasts. The micropatterned nanoribbon sheets may be a useful tool for directing cellular organization with defined alignment for regenerative medicine and drug screening applications.
authors.affiliation: WPI-Advanced Institute for Materials Research, Tohoku University, Sendai 980-8578, Japan; Department of Life Science and Medical Bioscience, Graduate School of Advanced Science and Engineering, Waseda University, Tokyo, Japan.
authors.forename: Toshinori
authors.initials: T
authors.lastname: Fujie
authors.affiliation: WPI-Advanced Institute for Materials Research, Tohoku University, Sendai 980-8578, Japan.
authors.forename: Xuetao
authors.initials: X
authors.lastname: Shi
authors.affiliation: WPI-Advanced Institute for Materials Research, Tohoku University, Sendai 980-8578, Japan.
authors.forename: Serge
authors.initials: S
authors.lastname: Ostrovidov
authors.affiliation: WPI-Advanced Institute for Materials Research, Tohoku University, Sendai 980-8578, Japan.
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Liang
authors.affiliation: WPI-Advanced Institute for Materials Research, Tohoku University, Sendai 980-8578, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Nakajima
authors.affiliation: Department of Chemistry & Division of Biomedical Engineering, Hong Kong University of Science & Technology, Hong Kong, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Chen
authors.affiliation: WPI-Advanced Institute for Materials Research, Tohoku University, Sendai 980-8578, Japan; Department of Chemistry & Division of Biomedical Engineering, Hong Kong University of Science & Technology, Hong Kong, China. Electronic address: chhkwu@ust.hk.
authors.forename: Hongkai
authors.initials: H
authors.lastname: Wu
authors.affiliation: WPI-Advanced Institute for Materials Research, Tohoku University, Sendai 980-8578, Japan; Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital Harvard Medical School, Harvard-MIT Division of Health Sciences and Technology Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA 02115, USA; Department of Physics, King Abdulaziz University, Jeddah 21569, Saudi Arabia. Electronic address: alik@rics.bwh.harvard.edu.
authors.forename: Ali
authors.initials: A
authors.lastname: Khademhosseini
mesh_terms: D000818:Animals; D002460:Cell Line; D015870:Gene Expression; D051379:Mice; D008855:Microscopy, Electron, Scanning; D018482:Muscle, Skeletal / Q000166:cytology* / Q000378:metabolism; D032446:Myoblasts / Q000166:cytology* / Q000378:metabolism; D049329:Nanostructures
keywords: Bilayer; Cell assembling; Cell orientation; Micropattern; Myoblasts; Nanoribbon sheet