title: Paired design study by real-time PCR: miR-378* and miR-145 are potent early diagnostic biomarkers of human colorectal cancer.
abstract: BACKGROUND
  Although microRNAs offer great potential as cancer biomarkers, effective clinical dignostics and tumor maker have not been verified to diagnose with colorectal cancer (CRC). The purpose of our study is to systematically assess the expression of miRNAs in matched cancer and normal tissue samples to identify promising diagnostic microRNA (miRNA) biomarkers for CRC.
  
  
  METHODS
  In our study, we examined by Real-Time PCR the expression levels of 96 mature miRNA in 32 CRC patients with differently expressed tumors versus normal colon tissues. Using enter and stepwise variable selection methods separately, conditional logistic regression was conducted to identify miRNAs associated with CRC. The classification performance of these indicators was assessed under the Fisher discriminant analysis. Receiver operating characteristic curve analyses were applied to obtain diagnostic utility of the differentially expressed miRNAs.
  
  
  RESULTS
  In this study, we confirmed 11 overexpressed miRNAs with no less than twofold difference, and 85 downexpressed miRNAs with up to 0.5-fold difference in CRC from 96 aberrantly expressed miRNAs being identified by real-time PCR. Conditional logistic regression results confirmed that miRNA-378 and miRNA-145 expression profile was statistically significant. The error diagnosis rate of these two miRNAs are 0.194 and 0.113, separeately, showing by discriminant analysis.
  
  
  CONCLUSIONS
  MiRNA-145 and miRNA-378* are potential biomarkers for early detection of CRC, which may help in diagnosing CRC in early period.
authors.affiliation: Guilin Medical College, Huang Cheng North 2 Road 109, Qixing District, Guilin, Guangxi, China. dielianhuayaya@163.com.
authors.forename: Juan
authors.initials: J
authors.lastname: Peng
authors.affiliation: General Hospital of Guangzhou Military Command of PLA, Liuhua Road 111, Guangzhou, Guangdong, China. pwkxiezy@163.com.
authors.forename: Zhengyong
authors.initials: Z
authors.lastname: Xie
authors.affiliation: General Hospital of Guangzhou Military Command of PLA, Liuhua Road 111, Guangzhou, Guangdong, China. chliyang2008@sina.com.
authors.forename: Liyang
authors.initials: L
authors.lastname: Cheng
authors.affiliation: General Hospital of Guangzhou Military Command of PLA, Liuhua Road 111, Guangzhou, Guangdong, China. zhangyuxin-53825@163.com.
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: General Hospital of Guangzhou Military Command of PLA, Liuhua Road 111, Guangzhou, Guangdong, China. 294821766@qq.com.
authors.forename: Junyong
authors.initials: J
authors.lastname: Chen
authors.affiliation: Guilin Medical College, Huang Cheng North 2 Road 109, Qixing District, Guilin, Guangxi, China. yhp268@126.com.
authors.forename: Hongping
authors.initials: H
authors.lastname: Yu
authors.affiliation: General Hospital of Guangzhou Military Command of PLA, Liuhua Road 111, Guangzhou, Guangdong, China. 51812843@qq.com.
authors.forename: Zehang
authors.initials: Z
authors.lastname: Li
authors.affiliation: General Hospital of Guangzhou Military Command of PLA, Liuhua Road 111, Guangzhou, Guangdong, China. 1367706921@qq.com.
authors.forename: Huixing
authors.initials: H
authors.lastname: Kang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D055088:Early Detection of Cancer; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D009367:Neoplasm Staging
chemical_list: D014408:Biomarkers, Tumor; C541010:MIRN145 microRNA, human; C526012:MIRN378 microRNA, human; D035683:MicroRNAs