title: [Effect of NaCl stress on physiological characteristics of Physalis alkekengi var. franchetii test-tube plantlet].
abstract: OBJECTIVE
  To investigate the effect of NaCl stress on the growth and physiological characteristics of Physalis alkekengi var. franchetii, and to discuss the mechanism of salt tolerance.
  
  
  METHODS
  The test-tube plantlet was cultured on the stress medium with NaCl (0.2%~1.0%), the content of proline, soluble sugar, soluble protein, chlorophyll and malondialdthyde (MDA), and membrane relative permeability, as well as the activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) were determined and analyzed.
  
  
  RESULTS
  The average height, stem diameter, root length, number of root and chlorophyll content gradually decreased at salinity less than or equal 0.6% NaCl, then decreased rapidly at salinity greater than or equal 0.8% NaCl, while the root formation was significantly inhibited. The content of free proline and SOD activities always increased with NaCl concentration increasing (0.2%~ 1.0%). Nevertheless, the contents of soluble sugar, soluble protein and the activities of CAT and POD increased firstly and then decreased, but the contents of soluble sugar and soluble protein were higher than those of the control group, whereas the content of MDA and membrane relative permeability accumulated slowly at salinity less than or equal 0.6% NaCl, then increased rapidly at salinity greater than 0.8% NaCl.
  
  
  CONCLUSIONS
  The test-tube plantlet has an adaptability to salty environment at salinity less than or equal 0.6% NaCl, and the test-tube plantlet can alleviate the salty injury by increasing the contents of osmotic regulation substances, such as soluble sugar and soluble protein, decreasing the osmotic potential, and increasing the activities of antioxidant enzymes.
authors.forename: Chao-qiang
authors.initials: CQ
authors.lastname: Zhang
authors.forename: Xing-long
authors.initials: XL
authors.lastname: Wang
authors.forename: Zhi-yuan
authors.initials: ZY
authors.lastname: Yang
authors.forename: Xiu-fang
authors.initials: XF
authors.lastname: Zheng
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D002241:Carbohydrates / Q000737:chemistry; D002374:Catalase / Q000378:metabolism; D002734:Chlorophyll / Q000737:chemistry; D009195:Peroxidase / Q000378:metabolism; D010544:Peroxidases / Q000378:metabolism; D032321:Physalis / Q000187:drug effects / Q000502:physiology*; D018517:Plant Roots / Q000254:growth & development; D011392:Proline / Q000737:chemistry; D054712:Salinity; D012965:Sodium Chloride / Q000494:pharmacology*; D013312:Stress, Physiological; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D002241:Carbohydrates; D002734:Chlorophyll; D012965:Sodium Chloride; D011392:Proline; D010544:Peroxidases; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase