title: ﻿﻿﻿﻿﻿Nitric Oxide Synthase Activity and Angiogenesis Measured by Expression of CD34 in Burns Treated With Chitosan Films ﻿.
abstract: ﻿﻿﻿﻿﻿﻿﻿﻿﻿ ﻿Nitric oxide (NO) signaling appears to play a vital role in wound healing associated to improve collagen and angiogenesis. A burn wound model was used to evaluate the effects of a chitosan films on histopathological features, nitric oxide synthase (NOS) activity, and quantification of neoformed capillaries assessed with CD34.
  
  
  METHODS
  Bilateral burns (n = 16) were made on adult Sprague-Dawley rats. The lesions on the right side of the rats were treated with chitosan films, and the lesions on the left side of the same rats were treated with gauze with NaCl 0.9% as a control.
  
  
  RESULTS
  Histological analysis revealed accelerated burn wound healing supported by significant differences in acute inflammation, collagen, and granulation tissue formation in chitosan-treated burns. Additionally, chitosan-treated burns were associated with higher CD34 immunoreactivity antibody supported by significant differences. This analysis of NOS activity was statistically significant on treated burns in the second treatment week. NOS results are associated with the highest collagen deposition, granulation tissue formation, and new capillary formation.
  
  
  CONCLUSIONS
  The use of chitosan on burns promoted re-epithelialization by means of angiogenic and NO release associated with higher cell infiltration into the wound bed during the proliferative phase.
authors.affiliation: ﻿Institute of Clinical Veterinary Sciences, University of Southern Chile; Email; javierojeda@uach.cl.
authors.forename: Javier E
authors.initials: JE
authors.lastname: Ojeda
authors.forename: Galo
authors.initials: G
authors.lastname: Cardenas
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Klassen
authors.affiliation: ﻿
authors.forename: Carlos
authors.initials: C
authors.lastname: von Plessing
authors.forename: Patricia
authors.initials: P
authors.lastname: Grez
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Sepulveda