title: Microbiota associated with pollen, bee bread, larvae and adults of solitary bee Osmia cornuta (Hymenoptera: Megachilidae).
abstract: Using cultivation-dependant method, we isolated 184 strains from fresh and old bee bread, pollen, larvae and adults of solitary bee Osmia cornuta. The 16S rDNA sequencing of 79 selected isolates gave the final species-specific identification of strains. Phylogenetic analysis indicated that microbiota isolated from five different sources were represented with 29 species within three different phyla, Firmicutes with 25 species, Actinobacteria with only one species and Proteobacteria with three species of Enterobacteriaceae. Bacterial biodiversity presented with Shannon-Wiener index (H') was highest in the alimentary tract of adults and old bee bread (H' = 2.43 and H' = 2.53, respectively) and in the same time no dominance of any species was scored. On the contrary, results obtained for Simpson index (D) showed that in pollen samples the dominant species was Pantoea agglomerans (D = 0.42) while in fresh bee bread that was Staphylococcus sp. (D = 0.27). We assume that microbial diversity detected in the tested samples of solitary bee O. cornuta probably come from environment.
authors.affiliation: Faculty of Biology,University of Belgrade,Studentski trg 16,11000 Belgrade,Serbia.
authors.forename: J
authors.initials: J
authors.lastname: Lozo
authors.affiliation: Faculty of Biology,University of Belgrade,Studentski trg 16,11000 Belgrade,Serbia.
authors.forename: T
authors.initials: T
authors.lastname: Berić
authors.affiliation: University of Belgrade - Institute of Molecular Genetics and Genetic Engineering,Vojvode Stepe 444a,P.O. Box 23,11010 Belgrade,Serbia.
authors.forename: A
authors.initials: A
authors.lastname: Terzić-Vidojević
authors.affiliation: Faculty of Biology,University of Belgrade,Studentski trg 16,11000 Belgrade,Serbia.
authors.forename: S
authors.initials: S
authors.lastname: Stanković
authors.affiliation: Faculty of Biology,University of Belgrade,Studentski trg 16,11000 Belgrade,Serbia.
authors.forename: D
authors.initials: D
authors.lastname: Fira
authors.affiliation: Faculty of Biology,University of Belgrade,Studentski trg 16,11000 Belgrade,Serbia.
authors.forename: L
authors.initials: L
authors.lastname: Stanisavljević
mesh_terms: D000818:Animals; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D001516:Bees / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D005838:Genotype; D007814:Larva / Q000382:microbiology; D011058:Pollen / Q000382:microbiology*; D011429:Propolis
chemical_list: D004269:DNA, Bacterial; D011429:Propolis
keywords: Osmia cornuta; microbiota