title: Plasma-electrochemical deposition of porous zirconia on titanium-based dental material and in vitro interactions with primary osteoblasts cells.
abstract: Three new porous zirconia-coated titanium materials using anodic plasma-electrochemical oxidation have been fabricated and characterized by scanning electron microscopy, electron probe microanalysis and X-ray diffraction. These ZrO2/TiO2 surfaces contained up to 43 wt% of ZrO2, 49 wt% TiO2 ( M1: - M3: ) and 8 wt% P2O5 ( M2: , M3: ). Zirconium titanate was detected as dominant microcrystalline phase. Primary human osteoblast cells were used for in vitro investigations. Cell proliferation and immunohistochemical analyses of morphology and expression of bone sialoprotein and osteocalcin were performed. Novel coatings M2: and M3: were shown to induce proliferation and expression of osteocalcin and bone sialoprotein to the extent comparable to that of Ticer, a material already employed in clinical practice.
authors.affiliation: Department of Oral, Maxillary, Facial and Reconstructive Plastic Surgery, University Hospital of Leipzig, Leipzig, Germany milena.kaluderovic@medizin.uni-leipzig.de.
authors.forename: Milena R
authors.initials: MR
authors.lastname: Kaluđerović
authors.affiliation: ZL Microdent, Breckerfeld, Germany Department of Chemistry, Technical University of Chemnitz, Germany.
authors.forename: Joachim P
authors.initials: JP
authors.lastname: Schreckenbach
authors.affiliation: Department of Oral, Maxillary, Facial and Reconstructive Plastic Surgery, University Hospital of Leipzig, Leipzig, Germany.
authors.forename: Hans-Ludwig
authors.initials: HL
authors.lastname: Graf
mesh_terms: D049109:Cell Proliferation / Q000502:physiology; D048429:Cell Size; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis; D003764:Dental Materials / Q000737:chemistry*; D004595:Electroplating / Q000379:methods*; D006801:Humans; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D058626:Plasma Gases / Q000737:chemistry; D016062:Porosity; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*; D015040:Zirconium / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D003764:Dental Materials; D058626:Plasma Gases; C009495:titanium dioxide; D015040:Zirconium; D014025:Titanium; C028541:zirconium oxide
keywords: Zirconia; anodic plasma-electrochemical oxidation; bone sialoprotein; implants; osteoblasts; osteocalcin