title: Cardiac and hepatic role of r-AtHSP70: basal effects and protection against ischemic and sepsis conditions.
abstract: Heat shock proteins (HSPs), highly conserved in all organisms, act as molecular chaperones activated by several stresses. The HSP70 class of stress-induced proteins is the most studied subtype in cardiovascular and inflammatory disease. Because of the high similarity between plant and mammalian HSP70, the aim of this work was to evaluate whether recombinant HSP70 of plant origin (r-AtHSP70) was able to protect rat cardiac and hepatic function under ischemic and sepsis conditions. We demonstrated for the first time that, in ex vivo isolated and perfused rat heart, exogenous r-AtHSP70 exerted direct negative inotropic and lusitropic effects via Akt/endothelial nitric oxide synthase pathway, induced post-conditioning cardioprotection via Reperfusion Injury Salvage Kinase and Survivor Activating Factor Enhancement pathways, and did not cause hepatic damage. In vivo administration of r-AtHSP70 protected both heart and liver against lipopolysaccharide-dependent sepsis, as revealed by the reduced plasma levels of interleukin-1β, tumour necrosis factor alpha, aspartate aminotransferase and alanine aminotransferase. These results suggest exogenous r-AtHSP70 as a molecular modulator able to protect myocardial function and to prevent cardiac and liver dysfunctions during inflammatory conditions.
authors.affiliation: Department of Biology, Ecology and Earth Sciences, University of Calabria, Arcavacata di Rende (CS), Italy.
authors.forename: Teresa
authors.initials: T
authors.lastname: Pasqua
authors.affiliation: Department of Biology, Ecology and Earth Sciences, University of Calabria, Arcavacata di Rende (CS), Italy.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Filice
authors.affiliation: Department of Biology, Ecology and Earth Sciences, University of Calabria, Arcavacata di Rende (CS), Italy.
authors.forename: Rosa
authors.initials: R
authors.lastname: Mazza
authors.affiliation: Department of Biology, Ecology and Earth Sciences, University of Calabria, Arcavacata di Rende (CS), Italy.
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Quintieri
authors.affiliation: Department of Biology, Ecology and Earth Sciences, University of Calabria, Arcavacata di Rende (CS), Italy.|National Institute of Cardiovascular Research, Bologna, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Carmela Cerra
authors.affiliation: ALSIA-Research Center Metapontum Agrobios, Metaponto (MT), Italy.
authors.forename: Rina
authors.initials: R
authors.lastname: Iannacone
authors.affiliation: ALSIA-Research Center Metapontum Agrobios, Metaponto (MT), Italy.
authors.forename: Donato
authors.initials: D
authors.lastname: Melfi
authors.affiliation: Department of Biology, Ecology and Earth Sciences, University of Calabria, Arcavacata di Rende (CS), Italy.
authors.forename: Cesare
authors.initials: C
authors.lastname: Indiveri
authors.affiliation: Department of Biology, Ecology and Earth Sciences, University of Calabria, Arcavacata di Rende (CS), Italy.
authors.forename: Alfonsina
authors.initials: A
authors.lastname: Gattuso
authors.affiliation: Department of Biology, Ecology and Earth Sciences, University of Calabria, Arcavacata di Rende (CS), Italy.|National Institute of Cardiovascular Research, Bologna, Italy.
authors.forename: Tommaso
authors.initials: T
authors.lastname: Angelone
mesh_terms: D000818:Animals; D029681:Arabidopsis Proteins / Q000378:metabolism*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008969:Molecular Sequence Data; D009203:Myocardial Infarction / Q000473:pathology / Q000503:physiopathology; D017202:Myocardial Ischemia / Q000401:mortality* / Q000473:pathology / Q000503:physiopathology; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000378:metabolism*; D018805:Sepsis / Q000150:complications / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control*; D015398:Signal Transduction / Q000187:drug effects; D013599:Systole / Q000187:drug effects
chemical_list: D029681:Arabidopsis Proteins; D018840:HSP70 Heat-Shock Proteins; D008070:Lipopolysaccharides; D011994:Recombinant Proteins
keywords: Arabidopsis thaliana; cardioprotection; heart; heat shock protein; liver; sepsis