title: Impact of colonic mucosal lipoxin A4 synthesis capacity on healing in rats with dextran sodium sulfate-induced colitis.
abstract: Ulcerative colitis is a chronic inflammatory disease of the colon. This study evaluates the role of colonic mucosal lipoxin A4 (LXA4) synthesis in an experimental rat model of dextran sodium sulfate (DSS)-induced colitis. Wistar rats were randomly assigned to four groups: healthy controls, DSS-induced colitis with no or vehicle therapy, misoprostol or 5-aminosalicylic acid (5-ASA) therapy groups. Disease severity and colonic mucosal LXA4 synthesis was assessed specifically during the acute phase (day 5), chronic phase (day 15) and healing phases (day 19). Both misoprostol and 5-ASA reduced histopathologic score during the acute phase and reduced disease activity score at the healing phase. In addition, misoprostol reduced histopathologic score and colon weight/length ratio during the healing phase. Only misoprostol therapy increased colonic mucosal LXA4 synthesis. Furthermore, LXA4 levels correlated negatively with disease progression (R=-0.953). Collectively, our findings suggest that misoprostol-induced LXA4 synthesis may be favorable for the healing of ulcerative colitis.
authors.affiliation: Department of Pharmacology, Ankara University, Faculty of Medicine, Turkey.
authors.forename: Erol R
authors.initials: ER
authors.lastname: Ağış
authors.affiliation: Department of Pathology, Ankara University, Faculty of Medicine, Turkey.
authors.forename: Berna
authors.initials: B
authors.lastname: Savaş
authors.affiliation: Department of Pharmacology, Ankara University, Faculty of Medicine, Turkey. Electronic address: melli@medicine.ankara.edu.tr.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Melli
mesh_terms: D000818:Animals; D003092:Colitis / Q000139:chemically induced* / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D003106:Colon / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D016264:Dextran Sulfate / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D044045:Lipoxins / Q000378:metabolism*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D044045:Lipoxins; C040527:lipoxin A4; D016264:Dextran Sulfate
keywords: 5-Aminosalicylic acid; Dextran sodium sulfate induced-colitis; Inflammatory resolution; Lipoxin A(4); Misoprostol; Ulcerative colitis