title: Traction forces mediated by integrin signaling are necessary for definitive endoderm specification.
abstract: Pluripotent embryonic stem cells (ESCs) exert low-traction forces on their niche in vitro whereas specification to definitive endoderm in vivo coincides with force-mediated motility, suggesting a differentiation-mediated switch. However, the onset of contractility and extent to which force-mediated integrin signaling regulates fate choices is not understood. To address the requirement of tractions forces for differentiation, we examined mouse embryonic stem cell (ESC) specification towards definitive endoderm on fibrillar fibronectin containing a deformation-sensitive FRET probe. Inhibiting contractility resulted in an increase in the observed fibronectin FRET intensity ratio but also decreased the amount of phosphorylated nuclear SMAD2, leading to reduced expression of the definitive endoderm marker SOX17. By contrast ESCs maintained in pluripotency medium did not exert significant tractions against the fibronectin matrix. When laminin-111 was added to fibrillar matrices to improve the efficiency of definitive endoderm induction, ESCs decreased their fibronectin traction forces in a laminin-dependent manner; blocking the laminin-binding α3-integrin restored fibronectin matrix deformation and reduced SOX17 expression and SMAD2 phosphorylation, probably because of compensation of inhibitory signaling from SMAD7 after 5 days in culture. These data imply that traction forces and integrin signaling are important regulators of early fate decisions in ESCs.
authors.affiliation: Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA Sanford Consortium for Regenerative Medicine, La Jolla, CA 92037, USA.
authors.forename: Hermes
authors.initials: H
authors.lastname: Taylor-Weiner
authors.affiliation: Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA.
authors.forename: Neeraja
authors.initials: N
authors.lastname: Ravi
authors.affiliation: Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA Sanford Consortium for Regenerative Medicine, La Jolla, CA 92037, USA Biomedical Sciences Program, University of California, San Diego, La Jolla, CA 92093, USA aengler@ucsd.edu.
authors.forename: Adam J
authors.initials: AJ
authors.lastname: Engler
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D004707:Endoderm / Q000166:cytology*; D006801:Humans; D016023:Integrins / Q000378:metabolism*; D051379:Mice; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D011485:Protein Binding; D015398:Signal Transduction
chemical_list: D016023:Integrins
keywords: Embryonic stem cells; Endoderm; Extracellular matrix; Integrin signaling; SMAD