title: Improved DNA equilibrium binding affinity determinations of platinum(II) complexes using synchrotron radiation circular dichroism.
abstract: The binding affinity of a series of square planar platinum(II) compounds of the type [Pt(A(L))(I(L))](2+), where A(L) is 1,2-diaminoethane and I(L) are 1,10-phenanthroline (phen), 4-methyl-1,10-phenanthroline (4Mephen), 5-methyl-1,10-phenanthroline (5Mephen), 4,7-dimethyl-1,10-phenanthroline (47Me2phen), 5,6-dimethyl-1,10-phenanthroline (56Me2phen) or 3,4,7,8-tetramethyl-1,10-phenanthroline (3478Me4phen) has been reinvestigated using Synchrotron Radiation Circular Dichroism (SRCD) spectroscopy. The additional peaks exhibited considerably greater intensity than those observed between 200 and 400 nm affording additional binding affinity determinations. In addition, the authors have reviewed the various mathematical approaches used to estimate equilibrium binding constants and thereby demonstrate that their mathematical approach, implemented with Wolfram Mathematica, has merit over other methods.
authors.affiliation: Nanoscale Organisation and Dynamics Group, School of Science and Health, University of Western Sydney, Penrith, NSW 2751, Australia. J.Aldrich-Wright@uws.edu.au.
authors.forename: Dale L
authors.initials: DL
authors.lastname: Ang
authors.forename: Nykola C
authors.initials: NC
authors.lastname: Jones
authors.forename: Frank
authors.initials: F
authors.lastname: Stootman
authors.forename: Bahman
authors.initials: B
authors.lastname: Ghadirian
authors.forename: Janice R
authors.initials: JR
authors.lastname: Aldrich-Wright
mesh_terms: D002942:Circular Dichroism / Q000295:instrumentation*; D004247:DNA / Q000737:chemistry*; D009944:Organoplatinum Compounds / Q000032:analysis* / Q000737:chemistry*; D010618:Phenanthrolines / Q000737:chemistry; D017356:Synchrotrons
chemical_list: D009944:Organoplatinum Compounds; D010618:Phenanthrolines; D004247:DNA