title: Coomassie brilliant blue R-250 as a new surface-enhanced Raman scattering probe for prion protein through a dual-aptamer mechanism.
abstract: Surface-enhanced Raman scattering (SERS) spectra, which can provide large information about trace amount of chemical and biological species have been widely performed as a well-established tool in complex biological system. In this work, coomassie brilliant blue (R-250) with high affinity to proteins and high Raman activity was employed as a Raman reporter to probe prion protein (PrP) through a dual-aptamer mechanism, and thus an original strategy for PrP determination was proposed, which showed great potential to turn on the SERS response through specific recognition of anti-prion aptamers towards the target protein. Aptamers (Apt1 and Apt 2) recognizing distinct epitopes of PrP with high affinity were first conjugated to Ag@Si NPs, and Ag@Si-PrP/R-250-Ag@Si conjugates were obtained in the presence of PrP/R-250, inducing dramatically enhanced Raman signal. SERS responses enhanced with increasing amount of PrP and a linear equation of ISERS=6729.7+3091.2 cPrP was obtained in the range of 3.0-12.0×10(-9)M with the determination coefficient of 0.988. The proposed strategy is simple, rapid, and high specificity to probe protein-aptamer recognition in the solution.
authors.affiliation: Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China.
authors.forename: Ping Ping
authors.initials: PP
authors.lastname: Hu
authors.affiliation: Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.affiliation: College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhan
authors.affiliation: College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Lin Ling
authors.initials: LL
authors.lastname: Zheng
authors.affiliation: Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China; College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China. Electronic address: chengzhi@swu.edu.cn.
authors.forename: Cheng Zhi
authors.initials: CZ
authors.lastname: Huang
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D011328:Prions / Q000032:analysis*; D012394:Rosaniline Dyes / Q000737:chemistry*; D012825:Silicon / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D052157:Aptamers, Nucleotide; D011328:Prions; D012394:Rosaniline Dyes; D012834:Silver; C024757:Coomassie brilliant blue R; D006046:Gold; D012825:Silicon
keywords: Ag@Si NPs; Aptamer; Prion protein; Protein labeling; Surface-enhanced Raman scattering