title: Recombinant human brain natriuretic peptide ameliorates trauma-induced acute lung injury via inhibiting JAK/STAT signaling pathway in rats.
abstract: BACKGROUND
  JAK/STAT signal pathway plays an important role in the inflammation process of acute lung injury (ALI). This study aimed to investigate the correlation between recombinant human brain natriuretic peptide (rhBNP) and the JAK/STAT signaling pathway and to explore the protective mechanism of rhBNP against trauma-induced ALI.
  
  
  METHODS
  The arterial partial pressure in oxygen, lung wet-dry weight ratios, protein content in bronchoalveolar lavage fluid, the histopathologic of the lung, as well as the protein expressions of STAT1, JAK2, and STAT3 were detected.
  
  
  RESULTS
  Sprague-Dawley rats were randomly divided into five groups: a control group, a sham-operated group, an ALI group, an ALI + rhBNP group, and an ALI + AG490 group. At 4 hours, 12 hours, 1 day, 3 days, and 7 days after injury, injured lung specimens were harvested. rhBNP pretreatment significantly ameliorated hypoxemia and histopathologic changes and alleviated pulmonary edema in trauma-induced ALI rats. rhBNP pretreatment reduced the phosphorylated protein and total protein level of STAT1. Similarly to JAK-specific inhibitor AG490, rhBNP was shown to significantly inhibit the phosphorylation of JAK2 and STAT3 in rats with trauma-induced ALI.
  
  
  CONCLUSIONS
  Our experimental findings indicated that rhBNP can protect rats against trauma-induced ALI and that its underlying mechanism may be related to the inhibition of JAK/STAT signaling pathway activation.
authors.affiliation: From the Department of Emergency and Critical Care Medicine (Z.S., Y.G., M.H., H.J., Y.C.), The General Hospital of Shenyang Military District; and Department of Oral Medicine (X.Z.), Shenyang Medical College, Shenyang, Liaoning Province, China; and Department of Internal Medicine (M.L.), University of Nebraska Medical Center, Omaha, Nebraska.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Song
authors.forename: Xiu
authors.initials: X
authors.lastname: Zhao
authors.forename: Yan
authors.initials: Y
authors.lastname: Gao
authors.forename: Martin
authors.initials: M
authors.lastname: Liu
authors.forename: Mingxiao
authors.initials: M
authors.lastname: Hou
authors.forename: Hongxu
authors.initials: H
authors.lastname: Jin
authors.forename: Yan
authors.initials: Y
authors.lastname: Cui
mesh_terms: D055371:Acute Lung Injury / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D053613:Janus Kinase 1 / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D045283:Natriuretic Agents / Q000627:therapeutic use; D020097:Natriuretic Peptide, Brain / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins; D050791:STAT Transcription Factors / Q000187:drug effects* / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D014947:Wounds and Injuries / Q000150:complications*
chemical_list: D045283:Natriuretic Agents; D011994:Recombinant Proteins; D050791:STAT Transcription Factors; D020097:Natriuretic Peptide, Brain; C507906:Jak1 protein, rat; D053613:Janus Kinase 1