title: Synthesis and labeling of α-(2,9)-trisialic acid with cyanine dyes for imaging of glycan-binding receptors on living cells.
abstract: A sugar epitope, α-(2,9)-trisialic acid, was synthesized and labeled by cyanine dyes through Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC). The cyanine tagged oligosialic acid can be utilized as an efficient fluorescent probe to image the glycan-binding receptors on PC-12 cells. The distribution of Sia-binding immunoglobulin-like lectins (Siglecs) for α-(2,9)-trisialic acid was visualized by Cy3/Cy5 or FRET channel fluorescence imaging.
authors.affiliation: Department of Chemistry, Beijing Normal University, Beijing 100875, China. gwxing@bnu.edu.cn.
authors.forename: Xiao-tai
authors.initials: XT
authors.lastname: Zhang
authors.forename: Zhen-yuan
authors.initials: ZY
authors.lastname: Gu
authors.forename: Libing
authors.initials: L
authors.lastname: Liu
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
authors.forename: Guo-wen
authors.initials: GW
authors.lastname: Xing
mesh_terms: D000818:Animals; D002232:Carbocyanines / Q000138:chemical synthesis* / Q000737:chemistry*; D002470:Cell Survival; D005456:Fluorescent Dyes / Q000737:chemistry*; D015394:Molecular Structure; D016716:PC12 Cells; D011134:Polysaccharides / Q000378:metabolism*; D051381:Rats; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000378:metabolism*; D012794:Sialic Acids / Q000138:chemical synthesis* / Q000737:chemistry*; D014312:Trisaccharides / Q000138:chemical synthesis* / Q000737:chemistry*
chemical_list: D002232:Carbocyanines; D005456:Fluorescent Dyes; D011134:Polysaccharides; D011956:Receptors, Cell Surface; D012794:Sialic Acids; D014312:Trisaccharides