title: Spectral and lifetime domain measurements of rat brain tumors.
abstract: During glioblastoma surgery, delineation of the brain tumor margins is difficult because the infiltrated and normal tissues have the same visual appearance. We use a fiber-optical fluorescence probe for spectroscopic and time domain measurements to assist surgeon in differentiating the healthy and the infiltrated tissues. First study was performed on rats that were previously injected with tumorous cells. Measurements of endogenous tissue fluorescence were performed on fresh and fixed rat tumor brain slices. Spectral characteristics, fluorescence redox ratios and fluorescence lifetime measurements were analyzed. The study aimed at defining an optical index that can act as an indicator for discriminating healthy from tumorous tissue.
authors.affiliation: Laboratoire IMNC, UMR 8165, F-91405 Orsay, France ; Université Paris 7, F-75012 Paris, France.
authors.forename: D Abi
authors.initials: DA
authors.lastname: Haidar
authors.affiliation: Laboratoire IMNC, UMR 8165, F-91405 Orsay, France.
authors.forename: B
authors.initials: B
authors.lastname: Leh
authors.affiliation: Laboratoire IMNC, UMR 8165, F-91405 Orsay, France ; Neurosurgery Department, Centre Hospitalier Sainte-Anne, Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Zanello
authors.affiliation: Laboratoire IMNC, UMR 8165, F-91405 Orsay, France.
authors.forename: R
authors.initials: R
authors.lastname: Siebert
keywords: (170.1610) Clinical applications; (170.6510) Spectroscopy, tissue diagnostics; (170.6920) Time-resolved imaging; (170.6935) Tissue characterization; (300.0300) Spectroscopy; (300.2530) Fluorescence, laser-induced