title: NFκB2 Gene as a Novel Candidate that Epigenetically Responds to Interval Walking Training.
abstract: Physical fitness has been reported to decrease the risk of lifestyle-related diseases. The present study evaluated genome-wide methylation under the hypothesis that interval walking training (IWT) imparted beneficial effects on health, particularly by epigenetically ameliorating susceptibility to inflammation. We screened DNA from peripheral blood samples via genome-wide microarray for genes whose methylation was affected by IWT, paying special attention to promoter regions, and identified over 40 hyper- or hypo-methylated genes following IWT that were not witnessed in controls. We next selected genes in which the degree of methylation change in the promoter region was correlated with energy consumption following IWT. In this way, we found the NFκB2 gene to have increased methylation in multiple regions of its promoter sequence following participation in an exercise regimen. Next, IWT-induced NFκB2 hyper-methylation was confirmed by a quantitative PyroSequencing assessment of methylation in samples obtained from independent subjects who also underwent IWT. The increase in NFκB2 gene promoter methylation by IWT indicates that this regimen may suppress pro-inflammatory cytokines. Thus, these results provide an additional line of evidence that IWT is advantageous in promoting health from an epigenetic perspective by ameliorating susceptibility to inflammation.
authors.affiliation: Department of Molecular Oncology, Institute of Pathogenesis and Disease Prevention, Shinshu University Graduate School of Medicine, Matsumoto, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Molecular Oncology, Institute of Pathogenesis and Disease Prevention, Shinshu University Graduate School of Medicine, Matsumoto, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Hashimoto
authors.affiliation: Department of Molecular Oncology, Institute of Pathogenesis and Disease Prevention, Shinshu University Graduate School of Medicine, Matsumoto, Japan.
authors.forename: C
authors.initials: C
authors.lastname: Fujii
authors.affiliation: Department of Molecular Oncology, Institute of Pathogenesis and Disease Prevention, Shinshu University Graduate School of Medicine, Matsumoto, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Hida
authors.affiliation: Department of Molecular Oncology, Institute of Pathogenesis and Disease Prevention, Shinshu University Graduate School of Medicine, Matsumoto, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ito
authors.affiliation: Department of Molecular Oncology, Institute of Pathogenesis and Disease Prevention, Shinshu University Graduate School of Medicine, Matsumoto, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Matsumura
authors.affiliation: Department of Molecular Oncology, Institute of Pathogenesis and Disease Prevention, Shinshu University Graduate School of Medicine, Matsumoto, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sakaizawa
authors.affiliation: Department of Advanced Medicine for Health Promotion Institute for Biomedical Sciences, Shinshu University Interdisciplinary Cluster for Cutting Edge Research, Matsumoto, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Morikawa
authors.affiliation: Department of Advanced Medicine for Health Promotion Institute for Biomedical Sciences, Shinshu University Interdisciplinary Cluster for Cutting Edge Research, Matsumoto, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Masuki
authors.affiliation: Department of Advanced Medicine for Health Promotion Institute for Biomedical Sciences, Shinshu University Interdisciplinary Cluster for Cutting Edge Research, Matsumoto, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Nose
authors.affiliation: Department of Aging Biology, Institute of Pathogenesis and Disease Prevention, Shinshu University Graduate School of Medicine, Matsumoto, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Higuchi
authors.affiliation: Department of Sports and Health Sciences, Faculty of Human Health Science, Matsumoto University, Matsumoto, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nakajima
authors.affiliation: Department of Molecular Oncology, Institute of Pathogenesis and Disease Prevention, Shinshu University Graduate School of Medicine, Matsumoto, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Taniguchi
mesh_terms: D000368:Aged; D000375:Aging / Q000235:genetics*; D019175:DNA Methylation; D004734:Energy Metabolism / Q000235:genetics*; D055106:Genome-Wide Association Study; D006801:Humans; D007249:Inflammation / Q000235:genetics / Q000517:prevention & control; D008875:Middle Aged; D052003:NF-kappa B p52 Subunit / Q000235:genetics*; D010806:Physical Education and Training / Q000379:methods*; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D016138:Walking / Q000502:physiology*
chemical_list: D052003:NF-kappa B p52 Subunit; C498206:NFKB2 protein, human