title: Failure of interferon-γ pre-treated mesenchymal stem cell treatment in a patient with Crohn's disease.
abstract: Mesenchymal stem cells (MSC) are cells of stromal origin which exhibit unlimited self-renewal capacity and pluripotency in vitro. It has recently been observed that MSC may also exert a profound immunosuppressive and anti-inflammatory effect both in vitro and in vivo with consequent potential use in autoimmune disorders. We present the case of a patient suffering from childhood-onset, multidrug resistant and steroid-dependent Crohn's disease who underwent systemic infusions of MSC, which led to a temporary reduction in CCR4, CCR7 and CXCR4 expression by T-cells, and a temporary decrease in switched memory B-cells, In addition, following MSC infusion, lower doses of steroids were needed to inhibit proliferation of the patient's peripheral blood mononuclear cells. Despite these changes, no significant clinical benefit was observed, and the patient required rescue therapy with infliximab and subsequent autologous hematopoietic stem cell transplantation. The results of biological and in vitro observations after MSC use and the clinical effects of infusion are discussed, and a brief description is provided of previous data on MSC-based therapy in autoimmune disorders.
authors.affiliation: Andrea Taddio, Alessandro Ventura, Institute for Maternal and Child Health - IRCCS "Burlo Garofolo" - Trieste and University of Trieste, 34100 Trieste, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Taddio
authors.affiliation: Andrea Taddio, Alessandro Ventura, Institute for Maternal and Child Health - IRCCS "Burlo Garofolo" - Trieste and University of Trieste, 34100 Trieste, Italy.
authors.forename: Alberto
authors.initials: A
authors.lastname: Tommasini
authors.affiliation: Andrea Taddio, Alessandro Ventura, Institute for Maternal and Child Health - IRCCS "Burlo Garofolo" - Trieste and University of Trieste, 34100 Trieste, Italy.
authors.forename: Erica
authors.initials: E
authors.lastname: Valencic
authors.affiliation: Andrea Taddio, Alessandro Ventura, Institute for Maternal and Child Health - IRCCS "Burlo Garofolo" - Trieste and University of Trieste, 34100 Trieste, Italy.
authors.forename: Ettore
authors.initials: E
authors.lastname: Biagi
authors.affiliation: Andrea Taddio, Alessandro Ventura, Institute for Maternal and Child Health - IRCCS "Burlo Garofolo" - Trieste and University of Trieste, 34100 Trieste, Italy.
authors.forename: Giuliana
authors.initials: G
authors.lastname: Decorti
authors.affiliation: Andrea Taddio, Alessandro Ventura, Institute for Maternal and Child Health - IRCCS "Burlo Garofolo" - Trieste and University of Trieste, 34100 Trieste, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: De Iudicibus
authors.affiliation: Andrea Taddio, Alessandro Ventura, Institute for Maternal and Child Health - IRCCS "Burlo Garofolo" - Trieste and University of Trieste, 34100 Trieste, Italy.
authors.forename: Eva
authors.initials: E
authors.lastname: Cuzzoni
authors.affiliation: Andrea Taddio, Alessandro Ventura, Institute for Maternal and Child Health - IRCCS "Burlo Garofolo" - Trieste and University of Trieste, 34100 Trieste, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Gaipa
authors.affiliation: Andrea Taddio, Alessandro Ventura, Institute for Maternal and Child Health - IRCCS "Burlo Garofolo" - Trieste and University of Trieste, 34100 Trieste, Italy.
authors.forename: Raffaela
authors.initials: R
authors.lastname: Badolato
authors.affiliation: Andrea Taddio, Alessandro Ventura, Institute for Maternal and Child Health - IRCCS "Burlo Garofolo" - Trieste and University of Trieste, 34100 Trieste, Italy.
authors.forename: Alberto
authors.initials: A
authors.lastname: Prandini
authors.affiliation: Andrea Taddio, Alessandro Ventura, Institute for Maternal and Child Health - IRCCS "Burlo Garofolo" - Trieste and University of Trieste, 34100 Trieste, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Biondi
authors.affiliation: Andrea Taddio, Alessandro Ventura, Institute for Maternal and Child Health - IRCCS "Burlo Garofolo" - Trieste and University of Trieste, 34100 Trieste, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Ventura
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D057176:Compassionate Use Trials; D003424:Crohn Disease / Q000175:diagnosis / Q000276:immunology / Q000601:surgery*; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D007371:Interferon-gamma / Q000627:therapeutic use*; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000276:immunology; D012086:Reoperation; D016879:Salvage Therapy; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D017211:Treatment Failure
chemical_list: D007166:Immunosuppressive Agents; D007371:Interferon-gamma
keywords: Autoimmune disease; Crohn’s disease; Immune regulation; Interferon-γ pretreatment; Mesenchymal stem cells