title: Spectrofluorimetric determination of dopamine receptor antagonist LE 300 in mice plasma.
abstract: A simple, rapid and highly sensitive spectrofluorimetric method was developed for determination of a novel type of dopamine receptor antagonist LE300 in mouse plasma. The method is based on measuring the native fluorescence of LE-300 in methanol at 343 nm after excitation at 280 nm. The fluorescence concentration plot was rectilinear over the range of 3.5-100 ng/mL with a lower detection limit of 1.0 ng/mL and quantification limit of 3.5 ng/mL. The method was statistically validated for linearity, accuracy, precision and selectivity according to the International Conference on Harmonization guidelines. The accuracy and precision results was expressed as % recovery and relative standard deviation (RSD). The accuracy for LE-300 was in the range 95.5-103.6% and RSD values were in the range of 0.21-1.55% of the theoretical value. The method was successfully applied to the analysis of LE-300 in mice plasma. The results were compared statistically with those obtained by the reported method and were found to be in good agreement, which could be applied in a pharmacokinetic study.
authors.affiliation: Pharmaceutical Chemistry Department, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh, 11451, Saudi Arabia.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Hefnawy
authors.affiliation: Pharmaceutical Chemistry Department, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh, 11451, Saudi Arabia.
authors.forename: Mostafa
authors.initials: M
authors.lastname: Mohamed
authors.affiliation: Pharmaceutical Chemistry Department, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh, 11451, Saudi Arabia.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Abunassif
authors.affiliation: Pharmaceutical Chemistry Department, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh, 11451, Saudi Arabia.
authors.forename: Amer
authors.initials: A
authors.lastname: Alanazi
authors.affiliation: Pharmaceutical Chemistry Department, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh, 11451, Saudi Arabia.
authors.forename: Abdulrahman
authors.initials: A
authors.lastname: Al-Majed
authors.affiliation: Institut fur Pharmazie, Lehrstuhl fur Pharmazeutische/Medizinische Chemie, Friedrich Schiller, Universitat Jena, Jena, Germany.
authors.forename: Lehmann
authors.initials: L
authors.lastname: Jochen
authors.affiliation: Pharmaceutical Chemistry Department, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh, 11451, Saudi Arabia.
authors.forename: Gamal Abdel-Hafiz
authors.initials: GA
authors.lastname: Mostafa
mesh_terms: D000818:Animals; D018492:Dopamine Antagonists / Q000097:blood* / Q000737:chemistry; D007211:Indoles / Q000097:blood* / Q000737:chemistry; D051379:Mice; D015394:Molecular Structure; D013050:Spectrometry, Fluorescence
chemical_list: C409874:7-methyl-6,7,8,9,14,15-hexahydro-5H-benz(d)indolo(2,3-g)azecine; D018492:Dopamine Antagonists; D007211:Indoles
keywords: LE-300; Mice plasma; Spectrofluorimetry