title: Electrical stimulation by enzymatic biofuel cell to promote proliferation, migration and differentiation of muscle precursor cells.
abstract: Electrical stimulation is a very important biophysical cue for skeletal muscle maintenance and myotube formation. The absence of electrical signals from motor neurons causes denervated muscles to atrophy. Herein, we investigate for the first time the utility of an enzymatic biofuel cell (EBFC) as a promising means for mimicking native electrical stimulation. EBFC was set up using two different enzymes: one was glucose oxidase (GOX) used for the generation of anodic current followed by the oxidation of glucose; the other was Bilirubin oxidase (BOD) for the generation of cathodic current followed by the reduction of oxygen. We studied the behaviors of muscle precursor cells (MPCs) in terms of proliferation, migration and differentiation under different electrical conditions. The EBFC electrical stimulations significantly increased cell proliferation and migration. Furthermore, the electrical stimulations promoted the differentiation of cells into myotube formation based on expressions at the gene and protein levels. The EBFC set up, with its free forms adjustable to any implant design, was subsequently applied to the nanofiber scaffolding system. The MPCs were demonstrated to be stimulated in a similar manner as the 2D culture conditions, suggesting potential applications of the EBFC system for muscle repair and regeneration.
authors.affiliation: Department of Nanobiomedical Sciences and BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan 330-713, Republic of Korea; Institute of Tissue Regeneration Engineering, Dankook University, Cheonan 330-714, Republic of Korea.
authors.forename: Jae Ho
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Nanobiomedical Sciences and BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan 330-713, Republic of Korea; Department of Chemistry, College of Natural Science, Dankook University, Cheonan 330-714, Republic of Korea.
authors.forename: Won-Yong
authors.initials: WY
authors.lastname: Jeon
authors.affiliation: Department of Chemistry, College of Natural Science, Dankook University, Cheonan 330-714, Republic of Korea.
authors.forename: Hyug-Han
authors.initials: HH
authors.lastname: Kim
authors.affiliation: Department of Nanobiomedical Sciences and BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan 330-713, Republic of Korea; Institute of Tissue Regeneration Engineering, Dankook University, Cheonan 330-714, Republic of Korea.
authors.forename: Eun-Jung
authors.initials: EJ
authors.lastname: Lee
authors.affiliation: Department of Nanobiomedical Sciences and BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan 330-713, Republic of Korea; Institute of Tissue Regeneration Engineering, Dankook University, Cheonan 330-714, Republic of Korea; Department of Biomaterials Science, College of Dentistry, Dankook University, Cheonan 330-714, Republic of Korea. Electronic address: kimhw@dku.edu.
authors.forename: Hae-Won
authors.initials: HW
authors.lastname: Kim
mesh_terms: D000818:Animals; D001674:Bioelectric Energy Sources; D002454:Cell Differentiation; D002460:Cell Line; D002465:Cell Movement; D016764:Cell Polarity; D049109:Cell Proliferation; D004558:Electric Stimulation; D004566:Electrodes; D051379:Mice; D009132:Muscles / Q000166:cytology*
keywords: Electrical stimulation; Enzymatic biofuel cell; Implantable device; Muscle precursor cells; Muscle regeneration