title: A Brucella melitensis M5-90 wboA deletion strain is attenuated and enhances vaccine efficacy.
abstract: Brucella spp. are Gram-negative intracellular pathogens of both humans and animals that cause great economic burdens in developing countries. Live attenuated vaccines are the most efficient means for the prevention and control of animal Brucellosis. However, Brucella vaccines (strain M5-90 and others) have several drawbacks and do not allow serological differentiation between vaccinated and infected animals. A wboA mutant was derived from Brucella melitensis (B. melitensis) vaccine strain M5-90 and tested for virulence and protective efficiency. T-cell responses (CD4(+), CD8(+)), levels of immunoglobulin G (IgG), and cytokine production were observed. WboA was also assessed as a diagnostic marker for Brucellosis. B. melitensis strain M5-90ΔwboA exhibited reduced survival in murine macrophages (RAW 264.7) and BALB/c mice and induced protective immunity in mice comparable to that from the parental strain M5-90. In mice, the wboA mutant elicited an anti-Brucella-specific IgG response and induced the secretion of gamma interferon (IFN-γ) and interleukin-2 (IL-2). In sheep, M5-90ΔwboA immunization induced the secretion of IFN-γ, and serum samples from sheep inoculated with M5-90ΔwboA were negative by Bengal Plate Test (RBPT) and Standard Tube Agglutination Test (STAT). In mice, probes against WboA antigen allowed for serological differentiation between natural infection and vaccination. The M5-90ΔwboA mutant is a potential attenuated live vaccine candidate against virulent B. melitensis 16M infection. It will be further evaluated in livestock.
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi 832003, Xinjiang, China.
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi 832003, Xinjiang, China.
authors.forename: Jing-Xue
authors.initials: JX
authors.lastname: Shi
authors.affiliation: Changji State Center for Disease Control and Prevention, Changji 831100, Xinjiang, China.
authors.forename: Wen-Dong
authors.initials: WD
authors.lastname: Fu
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi 832003, Xinjiang, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi 832003, Xinjiang, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi 832003, Xinjiang, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi 832003, Xinjiang, China.
authors.forename: Tian-Sen
authors.initials: TS
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi 832003, Xinjiang, China.
authors.forename: Chuang-Fu
authors.initials: CF
authors.lastname: Chen
authors.affiliation: School of Medicine, Shihezi University, Shihezi 832003, Xinjiang, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Guo
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi 832003, Xinjiang, China. Electronic address: allanzhh@sohu.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000097:blood*; D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology; D002004:Brucella Vaccine / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology; D017347:Brucella melitensis / Q000235:genetics / Q000276:immunology*; D002006:Brucellosis / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000382:microbiology; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000382:microbiology; D005260:Female; D017353:Gene Deletion; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D007371:Interferon-gamma / Q000096:biosynthesis / Q000097:blood; D007376:Interleukin-2 / Q000096:biosynthesis / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D014611:Vaccination; D014613:Vaccines, Attenuated
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D002004:Brucella Vaccine; D007074:Immunoglobulin G; D007376:Interleukin-2; D014613:Vaccines, Attenuated; D007371:Interferon-gamma
keywords: B. melitensis M5-90; Live attenuated; Vaccine candidate; wboA