title: [Influence of microcystin-LR on cell viability and surface characteristics of Pseudomonas putida].
abstract: In microcystin-LR (MC-LR) degradation system, the change in surface characteristics and cell viability of Pseudomonas putida was studied. The purpose of this study was to reveal the influence of MC-LR on P. putida and elucidate the toxicity of MC-LR on microorganisms. The result demonstrated that MC-LR enhanced the cytoplasmic membrane permeability, as well as affected the ion metabolism and protein release of P. putida. The soluble sugar and Na+, Cl-release increased with the rising concentration of MC-LR ranging from 0 mg x L(-1) to 2.0 mg x L(-1). Flow Cytometry Method(FCM) analysis revealed that MC-LR accelerated the death of P. putida, and the death rate increased with the ascending concentration of MC-LR. Compared with the control, the death rate on day 5 increased by nearly 30% when 2.5 mg x L(-1) MC-LR was added. Scanning electron microscopy (SEM) analysis showed that the cells were deformed under the toxicity of MC-LR. After 5-day exposure to 2.5 mg x L(-1) MC-LR, the majority of the cells were ruptured and the intracellular materials flew out. The cellular structure was severely damaged under this condition.
authors.forename: Ting-jin
authors.initials: TJ
authors.lastname: Deng
authors.forename: Jin-shao
authors.initials: JS
authors.lastname: Ye
authors.forename: Hui
authors.initials: H
authors.lastname: Peng
authors.forename: Zhi-chen
authors.initials: ZC
authors.lastname: Liu
authors.forename: Ze-hua
authors.initials: ZH
authors.lastname: Liu
authors.forename: Hua
authors.initials: H
authors.lastname: Yin
authors.forename: Shuo-na
authors.initials: SN
authors.lastname: Chen
mesh_terms: D008387:Marine Toxins; D050296:Microbial Viability; D052998:Microcystins / Q000737:chemistry*; D016958:Pseudomonas putida / Q000166:cytology*
chemical_list: D008387:Marine Toxins; D052998:Microcystins; C057862:cyanoginosin LR