title: The prion protein regulates beta-amyloid-mediated self-renewal of neural stem cells in vitro.
abstract: The beta-amyloid (Aβ) peptide and the Aβ-oligomer receptor, prion protein (PrP), both influence neurogenesis. Using in vitro murine neural stem cells (NSCs), we investigated whether Aβ and PrP interact to modify neurogenesis. Aβ imparted PrP-dependent changes on NSC self-renewal, with PrP-ablated and wild-type NSCs displaying increased and decreased cell growth, respectively. In contrast, differentiation of Aβ-treated NSCs into mature cells was unaffected by PrP expression. Such marked PrP-dependent differences in NSC growth responses to Aβ provides further evidence of biologically significant interactions between these two factors and an important new insight into regulation of NSC self-renewal in vivo.
authors.affiliation: Department of Pathology, The University of Melbourne, Melbourne Brain Centre, Melbourne, VIC, 3010, Australia. stevenjc@unimelb.edu.au.|Department of Medicine, Royal Melbourne Hospital, The University of Melbourne, Melbourne, VIC, 3010, Australia. stevenjc@unimelb.edu.au.|Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Melbourne, VIC, 3010, Australia. stevenjc@unimelb.edu.au.
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Collins
authors.affiliation: Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Melbourne, VIC, 3010, Australia. carolin.tumpach@florey.edu.au.
authors.forename: Carolin
authors.initials: C
authors.lastname: Tumpach
authors.affiliation: Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Melbourne, VIC, 3010, Australia. q.li@unimelb.edu.au.
authors.forename: Qiao-Xin
authors.initials: QX
authors.lastname: Li
authors.affiliation: Department of Pathology, The University of Melbourne, Melbourne Brain Centre, Melbourne, VIC, 3010, Australia. v.lewis@unimelb.edu.aul.
authors.forename: Victoria
authors.initials: V
authors.lastname: Lewis
authors.affiliation: Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Melbourne, VIC, 3010, Australia. ryan.t@florey.edu.au.
authors.forename: Timothy M
authors.initials: TM
authors.lastname: Ryan
authors.affiliation: Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Melbourne, VIC, 3010, Australia. blaine.roberts@florey.edu.au.
authors.forename: Blaine
authors.initials: B
authors.lastname: Roberts
authors.affiliation: The Florey Department of Neuroscience and Mental Health, The University of Melbourne, Melbourne, VIC, 3010, Australia. simon.drew@unimelb.edu.au.
authors.forename: Simon C
authors.initials: SC
authors.lastname: Drew
authors.affiliation: Department of Pathology, The University of Melbourne, Melbourne Brain Centre, Melbourne, VIC, 3010, Australia. v.lawson@unimelb.edu.au.
authors.forename: Victoria A
authors.initials: VA
authors.lastname: Lawson
authors.affiliation: Department of Pathology, The University of Melbourne, Melbourne Brain Centre, Melbourne, VIC, 3010, Australia. chaigh@unimelb.edu.au.
authors.forename: Cathryn L
authors.initials: CL
authors.lastname: Haigh
mesh_terms: D016229:Amyloid beta-Peptides / Q000494:pharmacology*; D000818:Animals; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D051379:Mice; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D058953:Neural Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D055495:Neurogenesis / Q000187:drug effects; D011328:Prions / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D016229:Amyloid beta-Peptides; D011328:Prions