title: Proteomic analysis in cardiovascular research.
abstract: Advances in mass spectrometry technology and bioinformatics using clinical human samples have expanded quantitative proteomics in cardiovascular research. There are two major proteomic strategies: namely, "gel-based" or "gel-free" proteomics coupled with either "top-down" or "bottom-up" mass spectrometry. Both are introduced into the proteomic analysis using plasma or serum sample targeting 'biomarker" searches of aortic aneurysm and tissue samples, such as from the aneurysmal wall, calcific aortic valve, or myocardial tissue, investigating pathophysiological protein interactions and post-translational modifications. We summarize the proteomic studies that analyzed human samples taken during cardiovascular surgery to investigate disease processes, in order to better understand the system-wide changes behind known molecular factors and specific signaling pathways.
authors.affiliation: Division of Thoracic and Cardiovascular Surgery, Department of Surgery, Shimane University Faculty of Medicine, 89-1 Enya-cho, Izumo, Shimane, 693-8501, Japan. toda@med.shimane-u.ac.jp.
authors.forename: Teiji
authors.initials: T
authors.lastname: Oda
authors.affiliation: Department of Biosignaling and Radioisotope Experiment, Interdisciplinary Center for Science Research, Organization for Research, Shimane University, Izumo, Shimane, Japan.
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Matsumoto
mesh_terms: D015415:Biomarkers / Q000097:blood; D002309:Cardiology / Q000379:methods*; D002318:Cardiovascular Diseases / Q000097:blood / Q000235:genetics*; D019295:Computational Biology / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D040901:Proteomics / Q000379:methods*; D015398:Signal Transduction / Q000235:genetics
chemical_list: D015415:Biomarkers
keywords: Aortic aneurysm; Biomarker; Cardiac valve; Proteomics; Surgery