title: Selective targeting of the IL23 pathway: Generation and characterization of a novel high-affinity humanized anti-IL23A antibody.
abstract: Herein, we describe the generation and characterization of BI 655066, a novel, highly potent neutralizing anti-interleukin-23 (IL23) monoclonal antibody in clinical development for autoimmune conditions, including psoriasis and Crohn's disease. IL23 is a key driver of the differentiation, maintenance, and activity of a number of immune cell subsets, including T helper 17 (Th17) cells, which are believed to mediate the pathogenesis of several immune-mediated disorders. Thus, IL23 neutralization is an attractive therapeutic approach. Designing an antibody for clinical activity and convenience for the patient requires certain properties, such as high affinity, specificity, and solubility. These properties were achieved by directed design of the immunization, lead identification, and humanization procedures. Favorable substance and pharmacokinetic properties were established by biophysical assessments and studies in cynomolgus monkeys.
authors.affiliation: a Department of Biotherapeutics Research; Boehringer Ingelheim Pharmaceuticals Inc. ; Ridgefield , CT , USA.
authors.forename: Sanjaya
authors.initials: S
authors.lastname: Singh
authors.forename: Rachel R
authors.initials: RR
authors.lastname: Kroe-Barrett
authors.forename: Keith A
authors.initials: KA
authors.lastname: Canada
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhu
authors.forename: Eliud
authors.initials: E
authors.lastname: Sepulveda
authors.forename: Helen
authors.initials: H
authors.lastname: Wu
authors.forename: Yaqin
authors.initials: Y
authors.lastname: He
authors.forename: Ernest L
authors.initials: EL
authors.lastname: Raymond
authors.forename: Jennifer
authors.initials: J
authors.lastname: Ahlberg
authors.forename: Lee E
authors.initials: LE
authors.lastname: Frego
authors.forename: Laura M
authors.initials: LM
authors.lastname: Amodeo
authors.forename: Katrina M
authors.initials: KM
authors.lastname: Catron
authors.forename: David H
authors.initials: DH
authors.lastname: Presky
authors.forename: Jeffrey H
authors.initials: JH
authors.lastname: Hanke
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000493:pharmacokinetics* / Q000494:pharmacology*; D057134:Antibodies, Neutralizing / Q000276:immunology / Q000494:pharmacology*; D003424:Crohn Disease / Q000188:drug therapy / Q000276:immunology; D016503:Drug Delivery Systems; D006801:Humans; D053760:Interleukin-23 Subunit p19 / Q000037:antagonists & inhibitors* / Q000276:immunology; D008252:Macaca fascicularis; D011565:Psoriasis / Q000188:drug therapy / Q000276:immunology; D058504:Th17 Cells / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; C508840:IL23A protein, human; D053760:Interleukin-23 Subunit p19; C000601773:risankizumab
keywords: ADCC, antibody-dependent cell-mediated cytotoxicity; AUC, analytical ultracentrifugation; BI 655066; CCG, Chemical Computing Group; CDRs, complementarity-determining regions; CH, constant region; Cκ, constant kappa; DMF, dimethylformamide; EOF, electro-osmotic flow; ESI, electrospray ionization; F, phenylalanine; G, glycine; GAHA, goat anti-human IgG gamma antibody; HCLF, high concentration liquid formulation; IL12, Interleukin 12; IL12RB1, IL12 receptor subunit beta 1; IL23, Interleukin-23; IL23R, IL23 receptor; JAK2, Janus kinase 2; PBS, phosphate-buffered saline; PK, pharmacokinetic; RU, resonance units; SEC, size-exclusion chromatography; SPR, surface plasmon resonance; Th17, T helper 17 cells; UV, ultraviolet; V, variable; VH, variable heavy; Vκ, variable kappa; Y, tyrosine; biophysical assessment; humanization; immunogen design; pharmacokinetic profile; tyk2, tyrosine kinase 2