title: Nematicidal activity of Annona crassiflora leaf extract on Caenorhabditis elegans.
abstract: BACKGROUND
  The aim of this work was to investigate the potential nematicidal activity of Annona crassiflora leaf extract against Caenorhabditis elegans.
  
  
  METHODS
  The hydroalcoholic leaf extract and its fractions (dichloromethane, ethyl acetate, methanol and water) were submitted to mobility assay against the roundworm Caenorhabditis elegans. GC-MS and NMR analysis were performed in order to identify metabolites.
  
  
  RESULTS
  The dichloromethane and ethyl acetate fractions showed to be the most active among the hydroalcoholic leaf extracts and its four fractions. The percentages of C. elegans larvae immobility were 98.13 and 89.66%, respectively, at a concentration of 1000 μg.mL(-1). Besides some amino acids, palmitic acid methyl ester, 2-isopropyl-5-methylcyclohexanol, oleic acid methyl esther, stearic acid methyl ester, quercetin and kaempferol were also identified in these fractions.
  
  
  CONCLUSIONS
  The results indicated that of A. crassiflora leaf ethanolic extract has a good potential as a source for natural nematicide.
authors.affiliation: Departamento de Química, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, MG, 31270-901, Brazil. alanvisconde@ufmg.br.
authors.forename: Alan Rodrigues Teixeira
authors.initials: AR
authors.lastname: Machado
authors.affiliation: Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Pampulha, CEP 31270-901, Belo Horizonte, Minas Gerais, Brazil. ferreirasr2008@hotmail.com.
authors.forename: Sebastião Rodrigo
authors.initials: SR
authors.lastname: Ferreira
authors.affiliation: Departamento de Química, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, MG, 31270-901, Brazil. fsmedeirs@gmail.com.
authors.forename: Felipe
authors.initials: F
authors.lastname: da Silva Medeiros
authors.affiliation: Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Pampulha, CEP 31270-901, Belo Horizonte, Minas Gerais, Brazil. rtfujiwara@gmail.com.
authors.forename: Ricardo Toshio
authors.initials: RT
authors.lastname: Fujiwara
authors.affiliation: Departamento de Química, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, MG, 31270-901, Brazil. peixe@qui.ufmg.br.
authors.forename: José Dias
authors.initials: JD
authors.lastname: de Souza Filho
authors.affiliation: Departamento de Química, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, MG, 31270-901, Brazil. lpimentaqui@gmail.com.
authors.forename: Lúcia Pinheiro Santos
authors.initials: LP
authors.lastname: Pimenta
mesh_terms: D000818:Animals; D031041:Annona / Q000737:chemistry*; D000871:Anthelmintics / Q000302:isolation & purification / Q000494:pharmacology*; D017173:Caenorhabditis elegans / Q000187:drug effects* / Q000502:physiology; D007814:Larva / Q000187:drug effects / Q000502:physiology; D008124:Locomotion / Q000187:drug effects; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D016019:Survival Analysis
chemical_list: D000871:Anthelmintics; D010936:Plant Extracts