title: The use of tissue microarrays for semiquantitative evaluation of ATPaseC1 expression is ineffective.
abstract: We described earlier the possible role of ATPaseC1 expression as a diagnostic and prognostic marker for oral cancer; others have reported its use for tumors of the lung and breast. We assessed ATPaseC1 expression in a sample of oral squamous cell carcinoma (OSCC) using tissue microarrays (TMAs) to analyze the relation between ATPaseC1 expression and clinical, histopathological and prognostic parameters. We performed a retrospective study of 48 cases of OSCC. We constructed TMAs using two different regions of each tumor. V-ATPaseC1 immunohistochemistry was performed and assessed semiquantitatively. ATPaseC1 staining was observed in most of the neoplastic cells in all tumors. Staining was diffusely cytoplasmic and, to a lesser extent, nuclear. The degree of concordance between the measurements performed in tissue microarray 1 (TMA1) and tissue microarray 2 (TMA2), as evaluated using the intra-class correlation coefficient (ICC), was low. We found great variability in the immunohistochemical staining of the different regions of each tumor. We found 16 cases with mild expression (33.3%), 20 with moderate expression (41.7%) and 12 with intense expression (25%). Differences in the clinical-pathological variables studied were not statistically significant. The difficulty of immunohistochemical evaluation, the heterogeneity of the carcinomas and the fact that evaluation of expression requires semiquantitative analysis render the reliability of the results obtained from TMA-based techniques questionable.
authors.affiliation: Oral Medicine, Oral Surgery and Implantology Unit, Faculty of Medicine and Dentistry, Institute of Sanitary Research of Santiago (IDIS) , Santiago de Compostela , Spain.
authors.forename: M
authors.initials: M
authors.lastname: Pérez-Sayáns
authors.forename: J M
authors.initials: JM
authors.lastname: Suárez-Peñaranda
authors.forename: J M
authors.initials: JM
authors.lastname: Aguirre-Urízar
authors.forename: M J
authors.initials: MJ
authors.lastname: Rodríguez-Tojo
authors.forename: F
authors.initials: F
authors.lastname: Barros-Angueira
authors.forename: M
authors.initials: M
authors.lastname: Gallas-Torreira
authors.forename: A
authors.initials: A
authors.lastname: García-García
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000201:enzymology* / Q000473:pathology; D005260:Female; D006258:Head and Neck Neoplasms / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000201:enzymology* / Q000473:pathology; D011379:Prognosis; D015203:Reproducibility of Results; D012189:Retrospective Studies; D000077195:Squamous Cell Carcinoma of Head and Neck; D046888:Tissue Array Analysis / Q000379:methods*; D025262:Vacuolar Proton-Translocating ATPases / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; C555946:ATP6V1C1 protein, human; D025262:Vacuolar Proton-Translocating ATPases
keywords: ATP6V1C1; ATPaseC1; TMA; oral squamous cell carcinoma; tissue microarray