title: Innate Lymphoid Cells Control Early Colonization Resistance against Intestinal Pathogens through ID2-Dependent Regulation of the Microbiota.
abstract: Microbiota-mediated effects on the host immune response facilitate colonization resistance against pathogens. However, it is unclear whether and how the host immune response can regulate the microbiota to mediate colonization resistance. ID2, an essential transcriptional regulator for the development of innate lymphoid cell (ILC) progenitors, remains highly expressed in differentiated ILCs with unknown function. Using conditionally deficient mice in which ID2 is deleted from differentiated ILC3s, we observed that these mutant mice exhibited greatly impaired gut colonization resistance against Citrobacter rodentium. Utilizing gnotobiotic hosts, we showed that the ID2-dependent early colonization resistance was mediated by interleukin-22 (IL-22) regulation of the microbiota. In addition to regulating development, ID2 maintained homeostasis of ILC3s and controlled IL-22 production through an aryl hydrocarbon receptor (AhR) and IL-23 receptor pathway. Thus, ILC3s can mediate immune surveillance, which constantly maintains a proper microbiota, to facilitate early colonization resistance through an ID2-dependent regulation of IL-22.
authors.affiliation: Department of Pathology and Committee on Immunology, The University of Chicago, Chicago, IL 60637, USA; Tsinghua University School of Medicine, Beijing 100084, China. Electronic address: guoxiaohuan@biomed.tsinghua.edu.cn.
authors.forename: Xiaohuan
authors.initials: X
authors.lastname: Guo
authors.affiliation: Department of Pathology and Committee on Immunology, The University of Chicago, Chicago, IL 60637, USA; Institute of Biophysics and The University of Chicago joint Group for Immunotherapy, Key Laboratory for Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Department of Pathology and Committee on Immunology, The University of Chicago, Chicago, IL 60637, USA.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institute for Cancer Genetics, Departments of Neurology and Pathology, Columbia University Medical Center, New York, NY 10032, USA.
authors.forename: Anna
authors.initials: A
authors.lastname: Lasorella
authors.affiliation: Department of Pathology and Committee on Immunology, The University of Chicago, Chicago, IL 60637, USA.
authors.forename: Barbara L
authors.initials: BL
authors.lastname: Kee
authors.affiliation: Department of Pathology and Committee on Immunology, The University of Chicago, Chicago, IL 60637, USA; Institute of Biophysics and The University of Chicago joint Group for Immunotherapy, Key Laboratory for Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, China. Electronic address: yfu@uchicago.edu.
authors.forename: Yang-Xin
authors.initials: YX
authors.lastname: Fu
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D044082:Citrobacter rodentium / Q000276:immunology; D004756:Enterobacteriaceae Infections / Q000235:genetics / Q000276:immunology* / Q000382:microbiology / Q000473:pathology*; D005786:Gene Expression Regulation; D005856:Germ-Free Life / Q000276:immunology; D006706:Homeostasis / Q000276:immunology; D007113:Immunity, Innate; D051797:Inhibitor of Differentiation Protein 2 / Q000172:deficiency / Q000235:genetics / Q000276:immunology*; D007378:Interleukins / Q000235:genetics / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology / Q000382:microbiology / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D064307:Microbiota / Q000276:immunology; D018336:Receptors, Aryl Hydrocarbon / Q000235:genetics / Q000276:immunology*; D018123:Receptors, Interleukin / Q000235:genetics / Q000276:immunology; D015398:Signal Transduction; D000097043:Interleukin-22
chemical_list: D051797:Inhibitor of Differentiation Protein 2; D007378:Interleukins; D018336:Receptors, Aryl Hydrocarbon; D018123:Receptors, Interleukin; D000097043:Interleukin-22; C497452:Idb2 protein, mouse; C528688:interleukin-23 receptor, mouse