title: Preparative isolation and purification of lignans from Justicia procumbens using high-speed counter-current chromatography in stepwise elution mode.
abstract: Lignans, which are recognized as main constituents in Justicia procumbens, have attracted considerable attention due to their pharmacological activities, including antitumor, anti-hepatitic, cytotoxic, anti-microbial, and anti-virus properties. Preparative high-speed counter-current chromatography (HSCCC) was successfully applied to the isolation and purification of four lignans (justicidin B (1), justicidin A (2), 6'-hydroxyjusticidin C (3) and lignan J1 (4)) from J. procumbens using stepwise elution with a pair of two-phase solvent systems composed of n-hexane-ethyl acetate-methanol-water at (1.3:1:1.3:1, v/v) and (2.5:1:2.5:1, v/v). The preparative HSCCC separation was performed on 300 mg of crude sample yielding compounds 1 (19.7 mg), 2 (9.86 mg), 3 (11.26 mg), and 4 (2.54 mg) in a one-step separation, with purities over 95% as determined by HPLC. The structures of these compounds were identified by MS, 1H-NMR and 13C-NMR. This is the first report on the application of HSCCC to the efficient separation of lignans from J. procumbens.
authors.affiliation: Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Ningbo 315211, China. zhoupeijuanhaha@163.com.
authors.forename: Peijuan
authors.initials: P
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Ningbo 315211, China. luoqijun@nbu.edu.cn.
authors.forename: Qijun
authors.initials: Q
authors.lastname: Luo
authors.affiliation: Laboratory of Marine Natural Products, School of Marine Sciences, Ningbo University, Ningbo 315211, China. huahua20062008@126.com.|College of Pharmacy, Jinan University, Guangzhou 510632, China. huahua20062008@126.com.
authors.forename: Lijian
authors.initials: L
authors.lastname: Ding
authors.affiliation: Laboratory of Marine Natural Products, School of Marine Sciences, Ningbo University, Ningbo 315211, China. 15888107865@163.com.
authors.forename: Fang
authors.initials: F
authors.lastname: Fang
authors.affiliation: Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Ningbo 315211, China. 23yuanye@163.com.
authors.forename: Ye
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Laboratory of Marine Natural Products, School of Marine Sciences, Ningbo University, Ningbo 315211, China. chenjuanjuan@nbu.edu.cn.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Chen
authors.affiliation: Laboratory of Marine Natural Products, School of Marine Sciences, Ningbo University, Ningbo 315211, China. zhangjinrong@nbu.edu.cn.
authors.forename: Jinrong
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Ningbo 315211, China. jinhaixiao@nbu.edu.cn.
authors.forename: Haixiao
authors.initials: H
authors.lastname: Jin
authors.affiliation: Laboratory of Marine Natural Products, School of Marine Sciences, Ningbo University, Ningbo 315211, China. heshan@nbu.edu.cn.
authors.forename: Shan
authors.initials: S
authors.lastname: He
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D027902:Justicia / Q000737:chemistry*; D017705:Lignans / Q000737:chemistry* / Q000302:isolation & purification*; D015394:Molecular Structure
chemical_list: D017705:Lignans