title: Effects of the essential metals copper and zinc in two freshwater detritivores species: Biochemical approach.
abstract: The input of metals into freshwater ecosystems from natural and anthropogenic sources impairs water quality and can lead to biological alterations in organisms and plants, compromising the structure and the function of these ecosystems. Biochemical biomarkers may provide early detection of exposure to contaminants and indicate potential effects at higher levels of biological organisation. The effects of 48h exposures to copper and zinc on Atyaephyra desmarestii and Echinogammarus meridionalis were evaluated with a battery of biomarkers of oxidative stress and the determination of ingestion rates. The results showed different responses of biomarkers between species and each metal. Copper inhibited the enzymatic defence system of both species without signs of oxidative damage. Zinc induced the defence system in E. meriodionalis with no evidence of oxidative damage. However, in A. desmarestii exposed to zinc was observed oxidative damage. In addition, only zinc had significantly reduced the ingestion rate and just for E. meridionalis. The value of the integrated biomarkers response increased with concentration of both metals, which indicates that might be a valuable tool to interpretation of data as a whole, as different parameters have different weight according to type of exposure.
authors.affiliation: CESAM and Departamento de Biologia, Universidade de Aveiro, Campus Universitário de Santiago, 3810-191 Aveiro, Portugal. Electronic address: cquintaneiro@ua.pt.
authors.forename: C
authors.initials: C
authors.lastname: Quintaneiro
authors.affiliation: Department of Chemistry and Geochemistry, Colorado School of Mines,1012 14th Street, Golden, CO 80401, USA.
authors.forename: J
authors.initials: J
authors.lastname: Ranville
authors.affiliation: CESAM and Departamento de Biologia, Universidade de Aveiro, Campus Universitário de Santiago, 3810-191 Aveiro, Portugal.
authors.forename: A J A
authors.initials: AJA
authors.lastname: Nogueira
mesh_terms: D033304:Amphipoda / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D003300:Copper / Q000633:toxicity*; D033364:Decapoda / Q000187:drug effects* / Q000378:metabolism; D005247:Feeding Behavior / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015032:Zinc / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D014874:Water Pollutants, Chemical; D003300:Copper; D015032:Zinc
keywords: Copper; Freshwater invertebrates; Integrated biomarker response; Oxidative stress; Zinc