title: Novel electrochemical dual-aptamer-based sandwich biosensor using molybdenum disulfide/carbon aerogel composites and Au nanoparticles for signal amplification.
abstract: A new electrochemical aptamer biosensor for the platelet-derived growth factor BB (PDGF-BB) detection has been developed based on the signal amplification of MoS2/carbon aerogel composites (MoS2/CA) and sandwich assay. A facile hydrothermal route assisted by L-cysteine was applied to synthesize CA incorporated flower-like MoS2 with the large surface active sites and good conductivity. The electrochemical aptasensor was constructed by sandwiching the PDGF-BB between a glassy carbon electrode modified with thiol-terminated PDGF-BB aptamer-1 (Apt1)/gold nanoparticles (AuNPs)/MoS2/CA and the AuNPs with thiol-terminated PDGF-BB aptamer-2 (Apt2) and 6-ferrocenyl hexanethiol (Fc). Fc-AuNPs-Apt2 acted as tracer and AuNPs/MoS2/CA were utilized as the biosensor platform to immobilize a large amount of capture aptamers, owing to their layered structure and high surface-to-volume ratio. Based on the sandwich format, a dual signal amplification strategy had been successfully developed with a wide linear response in the range of 0.001-10nM and a limit of detection of 0.3 pM. The developed assay demonstrated good selectivity and high sensitivity, indicating potential applications in bioanalysis and biomedicine.
authors.affiliation: College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.
authors.forename: Lin-Xia
authors.initials: LX
authors.lastname: Fang
authors.affiliation: College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China. Electronic address: kejinghuang@163.com.
authors.forename: Ke-Jing
authors.initials: KJ
authors.lastname: Huang
authors.affiliation: College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D000077214:Becaplermin; D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D004220:Disulfides / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods; D006046:Gold / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008982:Molybdenum / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry / Q000648:ultrastructure; D020574:Proto-Oncogene Proteins c-sis / Q000097:blood*
chemical_list: D052157:Aptamers, Nucleotide; D004220:Disulfides; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D002244:Carbon; D006046:Gold; D008982:Molybdenum; C082964:molybdenum disulfide
keywords: Au nanoparticles; MoS(2)/carbon aerogel composites; Platelet-derived growth factor BB; Sandwich assay; Signal amplification