title: Deregulation of dicer and mir-155 expression in liposarcoma.
abstract: Liposarcoma (LPS) is the most common soft tissue sarcoma. It has been demonstrated that mir-155 was the most overexpressed miRNA in well-differentiated LPS(WDLPS)/dedifferentiated LPS (DDLPS). The aim of this study is to evaluate the involvement of Dicer, Drosha and mir-155 in development of LPS and their possible role in stratification of different histological subtypes. Dicer, Drosha and mir-155 mRNA levels were analyzed in formalin-fixed paraffin-embedded specimens from patients diagnosed with 62 LPS and compared with samples of adipose tissues of healthy donors. The experimental data were obtained using qRT-PCR comparing Dicer, Drosha and mir-155 expression levels in tumor samples versus normal fat. The tumor samples from LPS patients showed a significantly lower Dicer expression versus normal adipose tissue, while Drosha levels did not differ. Concerning mir155 expression levels, our results demonstrated a significant mir-155 up-regulation in all LPS subtypes versus normal adipose tissue except for WDLS. These findings demonstrate for the first time that Dicer is deregulated in LPS and show that mir-155 is differentially expressed in LPS subgroups and it could be a promising tool to improve LPS disease stratification and differential diagnosis.
authors.affiliation: Medical Oncology, Campus Bio-Medico University of Rome, Rome, Italy.
authors.forename: Bruno
authors.initials: B
authors.lastname: Vincenzi
authors.affiliation: Translational Oncology Laboratory, Campus Bio-Medico University of Rome, Rome, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Iuliani
authors.affiliation: Translational Oncology Laboratory, Campus Bio-Medico University of Rome, Rome, Italy.
authors.forename: Alice
authors.initials: A
authors.lastname: Zoccoli
authors.affiliation: Medical Oncology, Campus Bio-Medico University of Rome, Rome, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Pantano
authors.affiliation: Translational Oncology Laboratory, Campus Bio-Medico University of Rome, Rome, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Fioramonti
authors.affiliation: Medical Oncology, Campus Bio-Medico University of Rome, Rome, Italy.
authors.forename: Delia
authors.initials: D
authors.lastname: De Lisi
authors.affiliation: Medical Oncology, Campus Bio-Medico University of Rome, Rome, Italy.
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Frezza
authors.affiliation: Pathology, Campus Bio-Medico University of Rome, Rome, Italy.
authors.forename: Carla
authors.initials: C
authors.lastname: Rabitti
authors.affiliation: Pathology, Campus Bio-Medico University of Rome, Rome, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Perrone
authors.affiliation: Pathology, Campus Bio-Medico University of Rome, Rome, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Onetti Muda
authors.affiliation: Department of Surgical, Oncological and Oral Sciences, University of Palermo, Palermo, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Russo
authors.affiliation: Sbarro Institute for Cancer Research and Molecular Medicine, College of Science and Technology, Temple University BioLife Science, Philadelphia, PA, USA.|Department of Medicine, Surgery & Neuroscience University of Siena, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Giordano
authors.affiliation: Medical Oncology, Campus Bio-Medico University of Rome, Rome, Italy.
authors.forename: Daniele
authors.initials: D
authors.lastname: Santini
authors.affiliation: Anatomic Pathology, General Hospital of Treviso, Treviso, Italy.
authors.forename: Angelo Paolo
authors.initials: AP
authors.lastname: Dei Tos
authors.affiliation: Medical Oncology, Campus Bio-Medico University of Rome, Rome, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Tonini
mesh_terms: D053487:DEAD-box RNA Helicases / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D005260:Female; D006801:Humans; D008080:Liposarcoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D046228:Microarray Analysis; D012189:Retrospective Studies; D043244:Ribonuclease III / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism
chemical_list: C524942:MIRN155 microRNA, human; D035683:MicroRNAs; C408087:DICER1 protein, human; D043244:Ribonuclease III; D053487:DEAD-box RNA Helicases
keywords: Dicer; Drosha; liposarcoma; mir-155