title: Sub-minute method for simultaneous determination of aspartame, cyclamate, acesulfame-K and saccharin in food and pharmaceutical samples by capillary zone electrophoresis.
abstract: This paper reports the development of a sub-minute separation method by capillary zone electrophoresis for the determination of aspartame, cyclamate, acesulfame-K and saccharin in food products and pharmaceutical samples. Separations were performed in a fused uncoated silica capillary with UV detection at 220nm. Samples and standards were injected hydrodynamically using the short-end injection procedure. The electrophoretic system was operated under constant voltage of -30kV. The background electrolyte was composed of 45mmolL(-1) 2-amino-2-(hydroxymethyl)-1,3-propanediol and 15mmolL(-1) benzoic acid at pH 8.4. The separation time for all analytes was less than 1min. Evaluation of analytical parameters of the method showed good linearity (r(2)>0.9972), limit of detection of 3.3-6.4mgL(-1), intermediate precision better than 9.75% (peak area of sample) and recovery in the range of 91-117%.
authors.affiliation: Department of Chemistry, Federal University of Santa Catarina, Florianopolis, SC, Brazil.
authors.forename: Jacqueline Pereira
authors.initials: JP
authors.lastname: Vistuba
authors.affiliation: Department of Chemistry, Federal University of Santa Catarina, Florianopolis, SC, Brazil.
authors.forename: Maressa Danielli
authors.initials: MD
authors.lastname: Dolzan
authors.affiliation: Department of Chemistry, Federal University of Santa Catarina, Florianopolis, SC, Brazil.
authors.forename: Luciano
authors.initials: L
authors.lastname: Vitali
authors.affiliation: Department of Chemistry, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.
authors.forename: Marcone Augusto Leal
authors.initials: MA
authors.lastname: de Oliveira
authors.affiliation: Department of Chemistry, Federal University of Santa Catarina, Florianopolis, SC, Brazil. Electronic address: gustavo.micke@ufsc.br.
authors.forename: Gustavo Amadeu
authors.initials: GA
authors.lastname: Micke
mesh_terms: D001218:Aspartame / Q000032:analysis*; D003494:Cyclamates / Q000032:analysis*; D004573:Electrolytes; D019075:Electrophoresis, Capillary / Q000379:methods; D005504:Food Analysis; D004364:Pharmaceutical Preparations / Q000032:analysis*; D012439:Saccharin / Q000032:analysis*; D013549:Sweetening Agents / Q000032:analysis*; D013843:Thiazines / Q000032:analysis*
chemical_list: D003494:Cyclamates; D004573:Electrolytes; D004364:Pharmaceutical Preparations; D013549:Sweetening Agents; D013843:Thiazines; D012439:Saccharin; C006362:acetosulfame; D001218:Aspartame
keywords: Artificial sweetener; Capillary zone electrophoresis; Fast method; Food products; Pharmaceutical preparations