title: Shell proteome of rhynchonelliform brachiopods.
abstract: Brachiopods are a phylum of marine invertebrates that have an external bivalved shell to protect their living tissues. With few exceptions, this biomineralized structure is composed of calcite, mixed together with a minor organic fraction, comprising secreted proteins that become occluded in the shell structure, once formed. This organic matrix is thought to display several functions, in particular, to control mineral deposition and to regulate crystallite shapes. Thus, identifying the primary structure of matrix proteins is a prerequisite for generating bioinspired materials with tailored properties. In this study, we employed a proteomic approach to identify numerous peptides that constitute the shell proteins, in three rhynchonellid brachiopods from different localities. Our results suggest that the shell protein repertoires identified thus far, differ from that of better known calcifying metazoans, such as molluscs.
authors.affiliation: UMR CNRS 6282, Biogéosciences, Université de Bourgogne, 21000 Dijon, France. Electronic address: francoise.immel@u-bourgogne.fr.
authors.forename: Françoise
authors.initials: F
authors.lastname: Immel
authors.affiliation: UMR CNRS 7207, CR2P, Département Histoire de la Terre, Muséum National d'Histoire Naturelle, 75005 Paris, France.
authors.forename: Danièle
authors.initials: D
authors.lastname: Gaspard
authors.affiliation: UMR CNRS 7245, MCAM, Plateau technique de Spectrométrie de Masse Bio-organique, Muséum National d'Histoire Naturelle, 75005 Paris, France.
authors.forename: Arul
authors.initials: A
authors.lastname: Marie
authors.affiliation: UMR CNRS 6282, Biogéosciences, Université de Bourgogne, 21000 Dijon, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Guichard
authors.affiliation: School of Geographical and Earth Sciences, University of Glasgow, G12 8QQ Glasgow, United Kingdom.
authors.forename: Maggie
authors.initials: M
authors.lastname: Cusack
authors.affiliation: UMR CNRS 6282, Biogéosciences, Université de Bourgogne, 21000 Dijon, France. Electronic address: frederic.marin@u-bourgogne.fr.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Marin
mesh_terms: D060105:Animal Shells / Q000378:metabolism*; D000818:Animals; D002113:Calcification, Physiologic / Q000502:physiology; D002119:Calcium Carbonate / Q000737:chemistry; D007448:Invertebrates / Q000378:metabolism*; D008903:Minerals / Q000378:metabolism; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D020543:Proteome / Q000737:chemistry* / Q000378:metabolism*; D040901:Proteomics / Q000379:methods
chemical_list: D008903:Minerals; D010455:Peptides; D020543:Proteome; D002119:Calcium Carbonate
keywords: Biomineralization; Brachiopods; Extracellular matrix; Proteomics; Shell