title: Backbone and side-chain (1)H, (15)N, (13)C assignment and secondary structure of BPSL1445 from Burkholderia pseudomallei.
abstract: BPSL1445 is a lipoprotein produced by the Gram-negative bacterium Burkholderia pseudomallei (B. pseudomallei), the etiological agent of melioidosis. Immunodetection assays against sera patients using protein microarray suggest BPSL1445 involvement in melioidosis. Herein we report backbone, side chain NMR assignment and secondary structure for the recombinant protein.
authors.affiliation: Biomolecular NMR Unit, c/o S. Raffaele Scientific Institute, Via Olgettina, 58, Milan, Italy.
authors.forename: Giacomo
authors.initials: G
authors.lastname: Quilici
authors.affiliation: Biomolecular NMR Unit, c/o S. Raffaele Scientific Institute, Via Olgettina, 58, Milan, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Berardi
authors.affiliation: Biomolecular NMR Unit, c/o S. Raffaele Scientific Institute, Via Olgettina, 58, Milan, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Gaudesi
authors.affiliation: Department of Biosciences, University of Milan, Via Celoria, 26, 20133, Milan, Italy.
authors.forename: Louise J
authors.initials: LJ
authors.lastname: Gourlay
authors.affiliation: Department of Biosciences, University of Milan, Via Celoria, 26, 20133, Milan, Italy.
authors.forename: Martino
authors.initials: M
authors.lastname: Bolognesi
authors.affiliation: Biomolecular NMR Unit, c/o S. Raffaele Scientific Institute, Via Olgettina, 58, Milan, Italy. musco.giovanna@hsr.it.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Musco
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D016957:Burkholderia pseudomallei / Q000378:metabolism*; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D009587:Nitrogen Isotopes; D017433:Protein Structure, Secondary; D066244:Proton Magnetic Resonance Spectroscopy
chemical_list: D001426:Bacterial Proteins; D009587:Nitrogen Isotopes
keywords: Biological warfare agent; Host-pathogen interactions; Infectious diseases; Melioidosis; Vaccine