title: Surface-imprinted nanoparticles prepared with a His-tag-anchored epitope as the template.
abstract: The specific recognition of biomolecules by artificial antibodies has inspired fascination among chemists and biologists. Herein, we propose a new method to prepare epitope-oriented surface-imprinted nanoparticles with high template utilization efficiency. Using a His-tag as the anchor to facilitate the epitope immobilization/removal and the self-polymerization of dopamine to control the imprinted shell thickness, the prepared epitope-imprinted nanoparticles show specific recognition of the target protein. Moreover, with improved hydrophilicity of the His-tag-anchored epitope, this method opens up a universal route for imprinting epitopes with various polarities.
authors.affiliation: ‡University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
authors.forename: Senwu
authors.initials: S
authors.lastname: Li
authors.forename: Kaiguang
authors.initials: K
authors.lastname: Yang
authors.affiliation: ‡University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
authors.forename: Jianxi
authors.initials: J
authors.lastname: Liu
authors.forename: Bo
authors.initials: B
authors.lastname: Jiang
authors.forename: Lihua
authors.initials: L
authors.lastname: Zhang
authors.forename: Yukui
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D004298:Dopamine / Q000737:chemistry; D000939:Epitopes / Q000737:chemistry*; D006639:Histidine / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D054802:Molecular Imprinting; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D013499:Surface Properties
chemical_list: D000939:Epitopes; D006639:Histidine; D004298:Dopamine