title: Distribution of N-methyl-(14)C-labeled selegiline in the rat.
abstract: Tissue distribution of selegiline including N-methyl-(14)C-selegiline was studied with three different techniques. Whole body autoradiography of labeled selegiline in rats completed the former results obtained in mice. Counting radioactivity by liquid scintillation method in various body compartments gave an in-depth numerical estimation of distribution, while RP-HPLC determination of selegiline determined the fate of intact, non-metabolized parent compound. Whole body autoradiography following 15 and 60 min of intraperitoneal application of N-methyl-(14)C-selegiline verified definite and time-dependent blood-brain penetration of selegiline. Quantitative determination of tissue concentrations by liquid scintillation and RP-HPLC methods following 5, 15, 60 and 180 min of intraperitoneal administration of selegiline unanimously verified both blood-brain and blood-testis penetration of the compound through the barrier.
authors.affiliation: Department of Pharmacodynamics, Semmelweis University, Budapest, Hungary. Electronic address: drtekes@gmail.com.
authors.forename: Kornélia
authors.initials: K
authors.lastname: Tekes
authors.affiliation: Department of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest, Hungary; National Research Institute for Radiobiology and Radiohygenie, Budapest, Hungary.
authors.forename: Zita
authors.initials: Z
authors.lastname: Pöstényi
authors.affiliation: Laboratory of Synthesis, Institute of Isotopes, Co., Ltd. Budapest, Hungary.
authors.forename: Erzsébet B
authors.initials: EB
authors.lastname: Faigl
authors.affiliation: Department of Pharmacodynamics, Semmelweis University, Budapest, Hungary.
authors.forename: Kálmán
authors.initials: K
authors.lastname: Magyar
authors.affiliation: Radiopharmacy Laboratories, Ltd., Budaörs, Hungary.
authors.forename: András
authors.initials: A
authors.lastname: Polyák
authors.affiliation: Department of Nuclear Medicine, University of Debrecen, Debrecen, Hungary.
authors.forename: György
authors.initials: G
authors.lastname: Trencsényi
authors.affiliation: National Research Institute for Radiobiology and Radiohygenie, Budapest, Hungary.
authors.forename: Lajos
authors.initials: L
authors.lastname: Balogh
authors.affiliation: Department of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest, Hungary. Electronic address: drkalasz@gmail.com.
authors.forename: Huba
authors.initials: H
authors.lastname: Kalász
mesh_terms: D000818:Animals; D001345:Autoradiography / Q000379:methods; D001921:Brain / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007553:Isotope Labeling / Q000379:methods; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012642:Selegiline / Q000378:metabolism*; D014018:Tissue Distribution / Q000502:physiology*
chemical_list: D012642:Selegiline
keywords: HPLC; Radiolabeled ligand; Rat; Selegiline; Whole body autoradiography