title: Investigation of 3D ordered macroporous carbon with different polymer coatings and their application as an oral vaccine carrier.
abstract: 3-D ordered macroporous carbon with different polymer coatings were developed as new oral vaccine immunological systems. Poly dimethyl diallyl ammonium (PDDA), polyethyleneimine (PEI) and chitosan (CTS), three different polymers with electropositive or adsorption-promoting properties, were chosen as the coating materials to endow the vaccine delivery systems with different surface properties. The bovine serum albumin (BSA) was used as a model vaccine. The three different polymer coated systems exhibited similar release rate which minimized the influence of release rate. The measured value of immunoglobulin G (IgG) titers suggested that the sustained release rate of BSA from polymer coated systems exhibited no strengthened effect on the immune response but could delay the appearance of the peak of the IgG titers compared with uncoated system. The electrostatic attraction between the mucosal and positively charged carrier would be useful during the whole immune experiment. In addition, using the coating material with the ability of enhancing mucosal adsorption was important in the mid-late period of immune. The immunoglobulin A (IgA) titers induced by the polymer coated systems were significantly higher than that induced by the oral BSA solution or i.m. BSA with Freund's complete adjuvant (FCA) which suggested the successful mucosal immune response of the three different coated systems. Overall, this work provides valuable information for the development of oral vaccine delivery system.
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, Liaoning Province 110016, PR China.
authors.forename: Qinfu
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, Liaoning Province 110016, PR China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, Liaoning Province 110016, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yue
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, Liaoning Province 110016, PR China.
authors.forename: Jiahao
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, Liaoning Province 110016, PR China.
authors.forename: Donghua
authors.initials: D
authors.lastname: Di
authors.affiliation: School of Medical Devices, Shenyang Pharmaceutical University, PR China.
authors.forename: Yikun
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Traditional Chinese Medicine, College of Traditional Chinese Medicine, Shenyang Pharmaceutical University, PR China.
authors.forename: Xinyi
authors.initials: X
authors.lastname: Shao
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, Liaoning Province 110016, PR China. Electronic address: silingwang@syphu.edu.cn.
authors.forename: Siling
authors.initials: S
authors.lastname: Wang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002244:Carbon / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D004339:Drug Compounding; D016503:Drug Delivery Systems; D005260:Female; D007111:Immunity, Cellular / Q000276:immunology; D018928:Immunity, Mucosal / Q000276:immunology; D007114:Immunization; D007070:Immunoglobulin A / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D011108:Polymers / Q000737:chemistry; D016062:Porosity; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012995:Solubility; D014612:Vaccines / Q000737:chemistry*
chemical_list: D004337:Drug Carriers; D007070:Immunoglobulin A; D011108:Polymers; D014612:Vaccines; D012710:Serum Albumin, Bovine; D002244:Carbon
keywords: 3-D ordered macroporous carbon; Chitosan; Electrostatic attraction; Oral immunity; Polyethyleneimine; Release rate