title: Letter: Analytical potential of the in-electrospray ionization source hydrogen/deuterium exchange for the investigation of oligonucleotides.
abstract: It has previously been reported that different conformations of oligonucleotides may be detected using a gas-phase hydrogen/deuterium (H/D) exchange performed in the collision cell of a mass spectrometer. The presence of different conformers was postulated based on the bimodal shape of the deuterium distribution and on the ion mobility spectrometry data. Here we implement an in-electrospray ionization source H/D exchange to detect the different conformations of oligonucleotides in the region of ion formation. We observed that the number of H/D exchanges depends considerably on the temperature of the desolvating capillary and varies from 25% at 50°C to 80% at 450°C, but no bimodality in the shape of the deuterium distribution was observed. Such results indicate that in the region of ion formation different conformations of oligonucleotide ions rapidly interconvert one into another.
authors.affiliation: Skolkovo Institute of Science and Technology, Novaya St. 100, Skolkovo 143025, Russian Federation. Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Leninskij pr. 38 k.2, 119334 Moscow, Russian Federation. Moscow Institute of Physics and Technology, 141700 Dolgoprudnyi, Moscow Region, Russian Federation. yura542@gmail.com.
authors.forename: Yury
authors.initials: Y
authors.lastname: Kostyukevich
authors.affiliation: Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Leninskij pr. 38 k.2, 119334 Moscow, Russian Federation. Moscow Institute of Physics and Technology, 141700 Dolgoprudnyi, Moscow Region, Russian Federation. konoleha@yandex.ru.
authors.forename: Alexey
authors.initials: A
authors.lastname: Kononikhin
authors.affiliation: Moscow Institute of Physics and Technology, 141700 Dolgoprudnyi, Moscow Region, Russian Federation. Emanuel Institute for Biochemical Physics Russian Academy of Sciences, Kosygina St. 4, 119334 Moscow, Russian Federation. hexapole@gmail.com.
authors.forename: Igor
authors.initials: I
authors.lastname: Popov
authors.affiliation: Moscow Institute of Physics and Technology, 141700 Dolgoprudnyi, Moscow Region, Russian Federation. Research Center for Obstetrics, Gynecology and Perinatology, 4 Oparin St., Moscow 117997, Russian Federation. aurum19@mail.ru.
authors.forename: Natalia
authors.initials: N
authors.lastname: Starodubtzevad
authors.affiliation: Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Leninskij pr. 38 k.2, 119334 Moscow, Russian Federation.. yura542@rambler.ru.
authors.forename: Stanislav
authors.initials: S
authors.lastname: Pekov
authors.affiliation: Moscow Institute of Physics and Technology, 141700 Dolgoprudnyi, Moscow Region, Russian Federation. Emanuel Institute for Biochemical Physics Russian Academy of Sciences, Kosygina St. 4, 119334 Moscow, Russian Federation. kukaev@mail.ru.
authors.forename: Eugene
authors.initials: E
authors.lastname: Kukaev
authors.affiliation: Institute for Energy Problems of Chemical Physics Russian Academy of Sciences Leninskij pr. 38 k.2, 119334 Moscow, Russian Federation. mariind@yandex.ru.
authors.forename: Maria
authors.initials: M
authors.lastname: Indeykina
authors.affiliation: Skolkovo Institute of Science and Technology, Novaya St. 100, Skolkovo 143025, Russian Federation. Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Leninskij pr. 38 k.2, 119334 Moscow, Russian Federation. Moscow Institute of Physics and Technology, 141700 Dolgoprudnyi, Moscow Region, Russian Federation. ennikolaev@rambler.ru.
authors.forename: Eugene
authors.initials: E
authors.lastname: Nikolaev
mesh_terms: D041961:Deuterium Exchange Measurement / Q000379:methods*; D009841:Oligonucleotides / Q000032:analysis* / Q000737:chemistry*; D013048:Specimen Handling / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013696:Temperature
chemical_list: D009841:Oligonucleotides