title: The involvement of insulin-like growth factor 2 binding protein 3 (IMP3) in pancreatic cancer cell migration, invasion, and adhesion.
abstract: BACKGROUND
  Over-expression of insulin-like growth factor 2 mRNA binding protein 3 (IMP3) is correlated with poor prognosis in pancreatic ductal adenocarcinoma (PDAC). Previous studies examining other cancer types have implicated IMP3 in the regulation of several cellular functions that are characteristic of tumour cells. However, the role of this oncofetal protein in PDAC progression remained unclear.
  
  
  METHODS
  Using siRNA, we examined the effect of IMP3 inhibition on the motility, invasive ability, and matrix adhesion of PDAC cells. In addition, we also evaluated the expression of cytoskeleton-associated genes following IMP depletion.
  
  
  RESULTS
  Knockdown of IMP3 significantly decreased the motility, invasion, and extracellular matrix adhesion of select PDAC cells in vitro. In addition, IMP3-depleted cells exhibited lower levels of CD44 protein and KIF11 mRNA. Moreover, we also observed a reduction in downstream RhoA signaling following IMP3 knockdown, indicating that IMP3 modulates the levels of proteins involved in cytoskeletal organization.
  
  
  CONCLUSIONS
  These results suggest that IMP3 facilitates PDAC progression by enhancing the pro-metastatic behaviour of tumour cells.
authors.affiliation: Department of Experimental Therapeutics, British Columbia Cancer Agency, 675 West 10th Avenue, Vancouver, BC, V5Z 1 L3, Canada. cpasiliao@gmail.com.
authors.forename: Clarissa C
authors.initials: CC
authors.lastname: Pasiliao
authors.affiliation: Department of Experimental Therapeutics, British Columbia Cancer Agency, 675 West 10th Avenue, Vancouver, BC, V5Z 1 L3, Canada. chewei68@hotmail.com.
authors.forename: Che-Wei A
authors.initials: CW
authors.lastname: Chang
authors.affiliation: Department of Experimental Therapeutics, British Columbia Cancer Agency, 675 West 10th Avenue, Vancouver, BC, V5Z 1 L3, Canada. bsutherland@bccrc.ca.
authors.forename: Brent W
authors.initials: BW
authors.lastname: Sutherland
authors.affiliation: Department of Experimental Therapeutics, British Columbia Cancer Agency, 675 West 10th Avenue, Vancouver, BC, V5Z 1 L3, Canada. svaldez@bccrc.ca.
authors.forename: Shannon M
authors.initials: SM
authors.lastname: Valdez
authors.affiliation: Department of Pathology and Laboratory Medicine, Faculty of Medicine, University of British Columbia, Vancouver, BC, V6T 2B5, Canada. david.schaeffer@vch.ca.|The Pancreas Centre BC, 2775 Laurel St., Vancouver, BC, V5Z 1M9, Canada. david.schaeffer@vch.ca.
authors.forename: David
authors.initials: D
authors.lastname: Schaeffer
authors.affiliation: Department of Experimental Therapeutics, British Columbia Cancer Agency, 675 West 10th Avenue, Vancouver, BC, V5Z 1 L3, Canada. dyapp@bccrc.ca.|The Pancreas Centre BC, 2775 Laurel St., Vancouver, BC, V5Z 1M9, Canada. dyapp@bccrc.ca.|Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, BC, V6T 1Z3, Canada. dyapp@bccrc.ca.
authors.forename: Donald T
authors.initials: DT
authors.lastname: Yapp
authors.affiliation: Department of Experimental Therapeutics, British Columbia Cancer Agency, 675 West 10th Avenue, Vancouver, BC, V5Z 1 L3, Canada. sylvia.ng@rmp.uhn.ca.|Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, BC, V6T 1Z3, Canada. sylvia.ng@rmp.uhn.ca.
authors.forename: Sylvia S W
authors.initials: SS
authors.lastname: Ng
mesh_terms: D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000235:genetics*; D021441:Carcinoma, Pancreatic Ductal / Q000235:genetics* / Q000473:pathology; D002448:Cell Adhesion / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D055786:Gene Knockout Techniques; D006801:Humans; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D012333:RNA, Messenger; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; C498780:IGF2BP3 protein, human; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins