title: OsKASI, a β-ketoacyl-[acyl carrier protein] synthase I, is involved in root development in rice (Oryza sativa L.).
abstract: The involvement of OsKASI in FA synthesis is found to play a critical role in root development of rice. The root system plays important roles in plant nutrient and water acquisition. However, mechanisms of root development and molecular regulation in rice are still poorly understood. Here, we characterized a rice (Oryza sativa L.) mutant with shortened roots due to a defect in cell elongation. Map-based cloning revealed that the mutation occurred in a putative 3-oxoacyl-synthase, an ortholog of β-ketoacyl-[acyl carrier protein] synthase I (KASI) in Arabidopsis, thus designated as OsKASI. OsKASI was found to be ubiquitously expressed in various tissues throughout the plant and OsKASI protein was localized in the plastid. In addition, OsKASI deficiency resulted in reduced fertility and a remarkable change in fatty acid (FA) composition and contents in roots and seeds. Our results demonstrate that involvement of OsKASI in FA synthesis is required for root development in rice.
authors.affiliation: College of Science and Technology, Ningbo University, Ningbo, 315211, People's Republic of China.
authors.forename: Wona
authors.initials: W
authors.lastname: Ding
authors.forename: Li
authors.initials: L
authors.lastname: Lin
authors.forename: Botao
authors.initials: B
authors.lastname: Zhang
authors.forename: Xianbo
authors.initials: X
authors.lastname: Xiang
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
authors.forename: Zhichong
authors.initials: Z
authors.lastname: Pan
authors.forename: Shihua
authors.initials: S
authors.lastname: Zhu
mesh_terms: D000213:Acyl Carrier Protein / Q000378:metabolism*; D032461:Chromosomes, Plant / Q000235:genetics; D003001:Cloning, Molecular; D005227:Fatty Acids / Q000096:biosynthesis; D005298:Fertility; D018506:Gene Expression Regulation, Plant; D005816:Genetic Complementation Test; D009154:Mutation / Q000235:genetics; D012275:Oryza / Q000254:growth & development* / Q000378:metabolism*; D010641:Phenotype; D063245:Plant Development; D010940:Plant Proteins / Q000302:isolation & purification / Q000378:metabolism*; D018517:Plant Roots / Q000254:growth & development*; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D000213:Acyl Carrier Protein; D005227:Fatty Acids; D010940:Plant Proteins