title: Immunogenic response to a recombinant pseudorabies virus carrying bp26 gene of Brucella melitensis in mice.
abstract: Brucellae are facultative intracellular bacterial pathogens of a zoonotic disease called brucellosis. Live attenuated vaccines are utilized for prophylaxis of brucellosis; however, they retain residual virulence to human and/or animals, as well as interfere with diagnosis. In this study, recombinant virus PRV ΔTK/ΔgE/bp26 was screened and purified. One-step growth curve assay showed that the titer of recombinant virus was comparable to the parent strain. Mice experiments showed the recombinant virus elicited high titer of humoral antibodies against Brucella detected by enzyme-linked immunosorbent assay and against PRV by serum neutralization test. The recombinant virus induced high level of Brucella-specific lymphocyte proliferation response and production of interferon gamma. Collectively, these data suggest that the bivalent virus was capable of inducing both humoral and cellular immunity, and had the potential to be a vaccine candidate to prevent Brucella and/or pseudorabies virus infections.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei Province, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Yao
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei Province, China.
authors.forename: Chang-Xian
authors.initials: CX
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei Province, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei Province, China.
authors.forename: Xian-Jin
authors.initials: XJ
authors.lastname: Xu
authors.affiliation: Department of Preventive Veterinary Medicine, College of Animal Science & Technology, Shihezi University, Shihezi city, Xinjiang Uyghur Autonomous Region, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Preventive Veterinary Medicine, College of Animal Science & Technology, Shihezi University, Shihezi city, Xinjiang Uyghur Autonomous Region, China.
authors.forename: Chuang-Fu
authors.initials: CF
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei Province, China. Electronic address: lzf6789@mail.hzau.edu.cn.
authors.forename: Zheng-Fei
authors.initials: ZF
authors.lastname: Liu
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D001428:Bacterial Vaccines / Q000235:genetics / Q000276:immunology*; D017347:Brucella melitensis / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D008565:Membrane Proteins / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011557:Pseudorabies / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000914:Antibodies, Viral; C106040:BP26 protein, Brucella; D001428:Bacterial Vaccines; D008565:Membrane Proteins; D014614:Vaccines, Synthetic; D014765:Viral Vaccines
keywords: Brucella melitensis; Immunogenicity; Pseudorabies virus; bp26