title: Microscale electrodes integrated on COP for real sample Campylobacter spp. detection.
abstract: Campylobacter spp. are responsible for acute bacterial diseases in human worldwide. Nowadays campilobacteriosis is considered the most common foodborne illness in the European Union. In this paper the first electrochemical genosensor based on thin-film gold electrodes deposited onto Cyclo Olefin Polymer (COP) substrates was fabricated for the detection of Campylobacter spp in food matrices. The sensing element is characterized by several surface techniques and the sensitivity of the biosensor have been studied. A good linear relationship was obtained for the concentrations of PCR amplicon of Campylobacter spp. between 1 and 25 nM with a limit of detection (LOD) of 90 pM. Real samples have been validated with poultry meat samples and results were comparable with the PCR product samples. This is the last step for the fabrication of a Lab on a Chip (LOC), a biodevice integrating DNA sensor technology into microfluidic system, believed to perform an automated and complete assay, including sample preparation, PCR amplification, and electrochemical detection of Campylobacter spp. in raw poultry meat samples.
authors.affiliation: CIC microGUNE, Goiru kalea, 9, Polo de Innovación Garaia, 20500 Mondragón, Spain. Electronic address: mmorant@cicmicrogune.es.
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Morant-Miñana
authors.affiliation: CIC microGUNE, Goiru kalea, 9, Polo de Innovación Garaia, 20500 Mondragón, Spain; IK4-Ikerlan, P° JMª Arizmendiarrieta 2, 20500 Arrasate-Mondragón, Gipuzkoa, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Elizalde
mesh_terms: D000818:Animals; D002167:Campylobacter / Q000235:genetics / Q000302:isolation & purification*; D003199:Computer Systems; D003217:Conductometry / Q000295:instrumentation*; D003516:Cycloparaffins / Q000737:chemistry*; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005504:Food Analysis / Q000295:instrumentation*; D005506:Food Contamination / Q000032:analysis; D008839:Microelectrodes; D011202:Poultry Products / Q000382:microbiology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D018511:Systems Integration
chemical_list: D003516:Cycloparaffins; D004269:DNA, Bacterial
keywords: CV; Campylobacter spp; Electrochemical analyses; Raw meat sample; SWV