title: Opposite expression of CYP51A1 and its natural antisense transcript AluCYP51A1 in adenovirus type 37 infected retinal pigmented epithelial cells.
abstract: Cytochrome P450 family member CYP51A1 is a key enzyme in cholesterol biosynthesis whose deregulation is implicated in numerous diseases, including retinal degeneration. Here we describe that HAdV-37 infection leads to downregulation of CYP51A1 expression and overexpression of its antisense non-coding Alu element (AluCYP51A1) in retinal pigment epithelium (RPE) cells. This change in gene expression is associated with a reversed accumulation of a positive histone mark at the CYP51A1 and AluCYP51A1 promoters. Further, transient AluCYP51A1 RNA overexpression correlates with reduced CYP51A1 mRNA accumulation. Collectively, our data suggest that AluCYP51A1 might control CYP51A1 gene expression in HAdV-37-infected RPE cells.
authors.affiliation: Department of Medical Biochemistry and Microbiology, Uppsala University, Husargatan 3, BMC, Box 582, 75123 Uppsala, Sweden.
authors.forename: Julia Maria Anna
authors.initials: JM
authors.lastname: Pickl
authors.affiliation: Department of Medical Biochemistry and Microbiology, Uppsala University, Husargatan 3, BMC, Box 582, 75123 Uppsala, Sweden.
authors.forename: Wael
authors.initials: W
authors.lastname: Kamel
authors.affiliation: Department of Medical Biochemistry and Microbiology, Uppsala University, Husargatan 3, BMC, Box 582, 75123 Uppsala, Sweden.
authors.forename: Sibel
authors.initials: S
authors.lastname: Ciftci
authors.affiliation: Department of Medical Biochemistry and Microbiology, Uppsala University, Husargatan 3, BMC, Box 582, 75123 Uppsala, Sweden.
authors.forename: Tanel
authors.initials: T
authors.lastname: Punga
authors.affiliation: Department of Medical Biochemistry and Microbiology, Uppsala University, Husargatan 3, BMC, Box 582, 75123 Uppsala, Sweden. Electronic address: goran.akusjarvi@imbim.uu.se.
authors.forename: Göran
authors.initials: G
authors.lastname: Akusjärvi
mesh_terms: D055595:Mechanical Phenomena; D018625:Microscopy, Atomic Force / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*
keywords: Adenovirus; Alu element; CYP51A1; Human adenovirus type 37; Retinal pigment epithelium