title: Mitochondrial Markers of Programmed Cell Death in Arabidopsis thaliana.
abstract: In plants, apoptosis-like programmed cell death (AL-PCD) is readily distinguished from other forms of programmed cell death (PCD) through a distinct morphology. Detection of cytochrome c release from mitochondria and changes in mitochondrial morphology are the earliest markers for detection of this form of PCD in plants. In this chapter we provide detailed technical methods for the visualization of both of these mitochondrial markers of AL-PCD in Arabidopsis.
authors.affiliation: School of Biology and Environmental Science, University College Dublin, Dublin 4, Ireland.
authors.forename: Theresa J
authors.initials: TJ
authors.lastname: Reape
authors.forename: Joanna
authors.initials: J
authors.lastname: Kacprzyk
authors.forename: Niall
authors.initials: N
authors.lastname: Brogan
authors.forename: Lee
authors.initials: L
authors.lastname: Sweetlove
authors.forename: Paul F
authors.initials: PF
authors.lastname: McCabe
mesh_terms: D017209:Apoptosis; D017360:Arabidopsis / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000378:metabolism*; D015153:Blotting, Western / Q000379:methods; D018929:Cell Culture Techniques / Q000379:methods; D002458:Cell Fractionation / Q000379:methods; D045304:Cytochromes c / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D008928:Mitochondria / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D029681:Arabidopsis Proteins; D045304:Cytochromes c