title: Comparative study of novel versus conventional two-wavelength spectrophotometric methods for analysis of spectrally overlapping binary mixture.
abstract: Smart spectrophotometric methods have been applied and validated for the simultaneous determination of a binary mixture of chloramphenicol (CPL) and prednisolone acetate (PA) without preliminary separation. Two novel methods have been developed; the first method depends upon advanced absorbance subtraction (AAS), while the other method relies on advanced amplitude modulation (AAM); in addition to the well established dual wavelength (DW), ratio difference (RD) and constant center coupled with spectrum subtraction (CC-SS) methods. Accuracy, precision and linearity ranges of these methods were determined. Moreover, selectivity was assessed by analyzing synthetic mixtures of both drugs. The proposed methods were successfully applied to the assay of drugs in their pharmaceutical formulations. No interference was observed from common additives and the validity of the methods was tested. The obtained results have been statistically compared to that of official spectrophotometric methods to give a conclusion that there is no significant difference between the proposed methods and the official ones with respect to accuracy and precision.
authors.affiliation: Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr el Aini Street, 11562 Cairo, Egypt; Pharmaceutical Chemistry Department, Faculty of Pharmaceutical Sciences & Pharmaceutical Industries, Future University, 12311 Cairo, Egypt.
authors.forename: Hayam M
authors.initials: HM
authors.lastname: Lotfy
authors.affiliation: Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr el Aini Street, 11562 Cairo, Egypt.
authors.forename: Maha A
authors.initials: MA
authors.lastname: Hegazy
authors.affiliation: Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr el Aini Street, 11562 Cairo, Egypt.
authors.forename: Mamdouh R
authors.initials: MR
authors.lastname: Rezk
authors.affiliation: Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr el Aini Street, 11562 Cairo, Egypt. Electronic address: yasminrostom@yahoo.com.
authors.forename: Yasmin Rostom
authors.initials: YR
authors.lastname: Omran
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis*; D000893:Anti-Inflammatory Agents / Q000032:analysis*; D002701:Chloramphenicol / Q000032:analysis*; D004338:Drug Combinations; D009883:Ophthalmic Solutions / Q000032:analysis*; D011239:Prednisolone / Q000031:analogs & derivatives* / Q000032:analysis; D013053:Spectrophotometry / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; D004338:Drug Combinations; D009883:Ophthalmic Solutions; D002701:Chloramphenicol; C009935:prednisolone acetate; D011239:Prednisolone
keywords: Advanced absorbance subtraction; Advanced amplitude modulation; Chloramphenicol and prednisolone acetate; Constant center; Spectrum subtraction; Two-wavelength