title: The uncovering of ESE-1 in human neutrophils: implication of its role in neutrophil function and survival.
abstract: Epithelium-specific Ets transcription factor 1 (ESE-1) is a member of the E26 transformation-specific family of transcription factors that has an epithelial-restricted constitutive expression but is induced by inflammatory stimuli in non-epithelial cells. Here we report that ESE-1 is constitutively expressed in human, but not in murine, neutrophils and that ESE-1 is modestly upregulated in septic patient neutrophils. In normal human neutrophils, ESE-1 was detected at both RNA and protein levels but was found to be an unstable nuclear protein ex vivo. ESE-1 transcription was also induced during all-trans retinoic acid-mediated HL-60 differentiation, a human promyelocytic cell line often used as an in vitro model of human neutrophils. Elf3-/- mice had normal neutrophils but a reduced number of circulating B-lymphocytes. These findings indicate a potential role of ESE-1 in regulating human neutrophil differentiation and function, and that it has different roles in the immune system of different species.
authors.affiliation: 1] SickKids Research Institute, Program in Physiology and Experimental Medicine, Peter Gilgan Centre for Research and Learning, SickKids Hospital, Toronto, Ontario, Canada [2] Laboratory Medicine & Pathobiology, University of Toronto, Toronto, Ontario, Canada [3] The Keenan Research Centre, Li Ka Shing Knowledge Institute, St Michael's Hospital, Toronto, Ontario, Canada.
authors.forename: C M
authors.initials: CM
authors.lastname: Lee
authors.affiliation: 1] The Keenan Research Centre, Li Ka Shing Knowledge Institute, St Michael's Hospital, Toronto, Ontario, Canada [2] Human Biology Department, University of Toronto, Toronto, Ontario, Canada.
authors.forename: S
authors.initials: S
authors.lastname: Gupta
authors.affiliation: 1] The Keenan Research Centre, Li Ka Shing Knowledge Institute, St Michael's Hospital, Toronto, Ontario, Canada [2] Department of Surgery, St Michael's Hospital, University of Toronto, Toronto, Ontario, Canada.
authors.forename: J
authors.initials: J
authors.lastname: Parodo
authors.affiliation: SickKids Research Institute, Program in Physiology and Experimental Medicine, Peter Gilgan Centre for Research and Learning, SickKids Hospital, Toronto, Ontario, Canada.
authors.forename: J
authors.initials: J
authors.lastname: Wu
authors.affiliation: 1] The Keenan Research Centre, Li Ka Shing Knowledge Institute, St Michael's Hospital, Toronto, Ontario, Canada [2] Department of Surgery, St Michael's Hospital, University of Toronto, Toronto, Ontario, Canada.
authors.forename: J C
authors.initials: JC
authors.lastname: Marshall
authors.affiliation: 1] SickKids Research Institute, Program in Physiology and Experimental Medicine, Peter Gilgan Centre for Research and Learning, SickKids Hospital, Toronto, Ontario, Canada [2] Laboratory Medicine & Pathobiology, University of Toronto, Toronto, Ontario, Canada.
authors.forename: J
authors.initials: J
authors.lastname: Hu
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D019891:Leukopoiesis; D051379:Mice; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D050783:Proto-Oncogene Proteins c-ets / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014212:Tretinoin / Q000494:pharmacology
chemical_list: D004268:DNA-Binding Proteins; C105529:ELF3 protein, human; C494436:Elf3 protein, mouse; D050783:Proto-Oncogene Proteins c-ets; D014157:Transcription Factors; D014212:Tretinoin