title: Pharmacological characterization of mechanisms involved in the vasorelaxation produced by rosuvastatin in aortic rings from rats with a cafeteria-style diet.
abstract: The present study aimed to investigate the possible influence of several inhibitors and blockers on the vascular effect produced by the acute in vitro application of rosuvastatin to phenylephrine-precontracted aortic rings from rats with a semi-solid, cafeteria-style (CAF) diet. It also aimed to examine the effects of rosuvastatin on the expression of endothelial nitric oxide synthase (eNOS), inducible nitric oxide synthase, constitutive cyclooxygenase, and inducible cyclooxygenase in aortic rings from rats with a CAF diet. From comparisons of the effect on phenylephrine-precontracted aortic rings extracted from rats with two different diets (a standard and a CAF diet), it was found that 10(-9) -10(-5) -mol/L rosuvastatin produced lower concentration-dependent vasorelaxation on rings from the CAF diet group. The vasorelaxant effect was unaffected by the vehicle, but it was significantly attenuated by 10(-5) -mol/L N(G) -nitro-l-arginine methyl ester, 10(-2) -mol/L tetraethylammonium, 10(-3) -mol/L 4-aminopyridine, 10(-7) -mol/L apamin plus 10(-7) -mol/L charybdotoxin, 10(-5) -mol/L indomethacin, or 10(-5) -mol/L cycloheximide. Moreover, in aortic rings from rats with a CAF diet, rosuvastatin enhanced the expression of eNOS, inducible nitric oxide synthase, constitutive cyclooxygenase, and inducible cyclooxygenase. The acute in vitro application of rosuvastatin to phenylephrine-precontracted aortic rings from rats with a CAF diet had a vasorelaxant effect. Overall, the present results suggest that the stimulation of eNOS, the opening of Ca(2+) -activated and voltage-activated K(+) channels, the stimulation of prostaglandin synthesis and enhanced protein levels of eNOS, inducible nitric oxide synthase, constitutive cyclooxygenase, and inducible cyclooxygenase are involved in this relaxant effect.
authors.affiliation: Section of Postgraduate Studies, Higher School of Medicine, National Polytechnic Institute, Mexico City, Mexico.|Department of Cellular Biology, National Intstitute of Perinatology, Mexico City, Mexico.
authors.forename: Jorge Skiold
authors.initials: JS
authors.lastname: López-Canales
authors.affiliation: Department of Cellular Biology, National Intstitute of Perinatology, Mexico City, Mexico.
authors.forename: Jair
authors.initials: J
authors.lastname: Lozano-Cuenca
authors.affiliation: Section of Postgraduate Studies, Higher School of Medicine, National Polytechnic Institute, Mexico City, Mexico.
authors.forename: Oscar Alberto
authors.initials: OA
authors.lastname: López-Canales
authors.affiliation: Department of Cellular Biology, National Intstitute of Perinatology, Mexico City, Mexico.
authors.forename: José Carlos
authors.initials: JC
authors.lastname: Aguilar-Carrasco
authors.affiliation: Section of Postgraduate Studies, Higher School of Medicine, National Polytechnic Institute, Mexico City, Mexico.
authors.forename: Lidia
authors.initials: L
authors.lastname: Aranda-Zepeda
authors.affiliation: Section of Postgraduate Studies, Higher School of Medicine, National Polytechnic Institute, Mexico City, Mexico.
authors.forename: Pedro
authors.initials: P
authors.lastname: López-Sánchez
authors.affiliation: Section of Postgraduate Studies, Higher School of Medicine, National Polytechnic Institute, Mexico City, Mexico.
authors.forename: Enrique Fernando
authors.initials: EF
authors.lastname: Castillo-Henkel
authors.affiliation: Section of Postgraduate Studies, Higher School of Medicine, National Polytechnic Institute, Mexico City, Mexico.
authors.forename: Ruth Mery
authors.initials: RM
authors.lastname: López-Mayorga
authors.affiliation: Section of Postgraduate Studies, Higher School of Medicine, National Polytechnic Institute, Mexico City, Mexico.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Valencia-Hernández
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects / Q000378:metabolism*; D004032:Diet / Q000009:adverse effects / Q000639:trends*; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism; D008297:Male; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D009924:Organ Culture Techniques; D051381:Rats; D017208:Rats, Wistar; D000068718:Rosuvastatin Calcium / Q000494:pharmacology*; D014664:Vasodilation / Q000187:drug effects / Q000502:physiology*; D014665:Vasodilator Agents / Q000494:pharmacology*
chemical_list: D014665:Vasodilator Agents; D000068718:Rosuvastatin Calcium; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat
keywords: aorta; endothelium; obesity; rate; rosuvastatin