title: Follicle dynamics and global organization in the intact mouse ovary.
abstract: Quantitative analysis of tissues and organs can reveal large-scale patterning as well as the impact of perturbations and aging on biological architecture. Here we develop tools for imaging of single cells in intact organs and computational approaches to assess spatial relationships in 3D. In the mouse ovary, we use nuclear volume of the oocyte to read out quiescence or growth of oocyte-somatic cell units known as follicles. This in-ovary quantification of non-growing follicle dynamics from neonate to adult fits a mathematical function, which corroborates the model of fixed oocyte reserve. Mapping approaches show that radial organization of folliculogenesis established in the newborn ovary is preserved through adulthood. By contrast, inter-follicle clustering increases during aging with different dynamics depending on size. These broadly applicable tools can reveal high dimensional phenotypes and age-related architectural changes in other organs. In the adult mouse pancreas, we find stochastic radial organization of the islets of Langerhans but evidence for localized interactions among the smallest islets.
authors.affiliation: Department of Ob/Gyn and Reproductive Sciences, Center for Reproductive Sciences, Eli and Edythe Broad, Center for Regeneration Medicine & Stem Cell Research, United States.
authors.forename: Mehlika
authors.initials: M
authors.lastname: Faire
authors.affiliation: Department of Ob/Gyn and Reproductive Sciences, Center for Reproductive Sciences, Eli and Edythe Broad, Center for Regeneration Medicine & Stem Cell Research, United States.
authors.forename: Amanda
authors.initials: A
authors.lastname: Skillern
authors.affiliation: Department of Ob/Gyn and Reproductive Sciences, Center for Reproductive Sciences, Eli and Edythe Broad, Center for Regeneration Medicine & Stem Cell Research, United States.
authors.forename: Ripla
authors.initials: R
authors.lastname: Arora
authors.affiliation: Department of Ob/Gyn and Reproductive Sciences, Center for Reproductive Sciences, Eli and Edythe Broad, Center for Regeneration Medicine & Stem Cell Research, United States.
authors.forename: Daniel H
authors.initials: DH
authors.lastname: Nguyen
authors.affiliation: Diabetes Center UCSF, 35 Medical Center Way, San Francisco, CA 94043, United States.
authors.forename: Jason
authors.initials: J
authors.lastname: Wang
authors.affiliation: Diabetes Center UCSF, 35 Medical Center Way, San Francisco, CA 94043, United States.
authors.forename: Chester
authors.initials: C
authors.lastname: Chamberlain
authors.affiliation: Diabetes Center UCSF, 35 Medical Center Way, San Francisco, CA 94043, United States.
authors.forename: Michael S
authors.initials: MS
authors.lastname: German
authors.affiliation: Department of Ob/Gyn and Reproductive Sciences, Center for Reproductive Sciences, Eli and Edythe Broad, Center for Regeneration Medicine & Stem Cell Research, United States.
authors.forename: Jennifer C
authors.initials: JC
authors.lastname: Fung
authors.affiliation: Department of Ob/Gyn and Reproductive Sciences, Center for Reproductive Sciences, Eli and Edythe Broad, Center for Regeneration Medicine & Stem Cell Research, United States. Electronic address: diana.laird@ucsf.edu.
authors.forename: Diana J
authors.initials: DJ
authors.lastname: Laird
mesh_terms: D000375:Aging; D000465:Algorithms; D000818:Animals; D005260:Female; D021621:Imaging, Three-Dimensional / Q000379:methods*; D007515:Islets of Langerhans / Q000502:physiology* / Q000648:ultrastructure; D051379:Mice; D008810:Mice, Inbred C57BL; D009865:Oocytes / Q000502:physiology* / Q000648:ultrastructure; D006080:Ovarian Follicle / Q000502:physiology* / Q000648:ultrastructure; D059010:Single-Cell Analysis / Q000379:methods*
keywords: Confocal imaging; Islet of Langerhans; Ovary; Pancreas; Primordial follicle; Whole tissue labeling