title: Production and anti-diabetic activity of soluble dietary fiber from apricot pulp by Trichoderma viride fermentation.
abstract: Soluble dietary fiber (SDF) was prepared by Trichoderma viride fermentation by using apricot pulp as the raw material. A four-factor and three-level response surface methodology was applied to optimize the fermentation conditions affecting the extraction rate of SDF. The optimum fermentation conditions were listed: crude enzyme volume, 9.59 mL g(-1); fermentation temperature, 43 °C; initial pH, 5.36; fermentation time, 6.47 h. Under these conditions, 15.69% yield was obtained and its relative error with the predicted theoretical value (15.87%) was 1.14%. The dietary fiber content of SDF was 84.0% whereas it was found to be 43.1% in apricot pulp flour. The anti-diabetic effect of apricot pulp SDF on rat models of diabetes was investigated. Both the blood glucose level and body weight were significantly changed in apricot pulp SDF-treated groups compared with the diabetic group (p < 0.01) after intragastric administration for 28 days. In addition, SDF elicited inhibitory effects on the α-glucosidase activity with an IC50 of 17.458 mg mL(-1). These results implied that apricot pulp SDF relieved the symptoms of diabetic rats.
authors.affiliation: College of Nature Conservation, Beijing Forestry University, Beijing 100083, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Cui
authors.forename: Xin
authors.initials: X
authors.lastname: Gu
authors.forename: Qiaohui
authors.initials: Q
authors.lastname: Zhang
authors.forename: Yangjie
authors.initials: Y
authors.lastname: Ou
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000201:enzymology / Q000378:metabolism; D004043:Dietary Fiber / Q000008:administration & dosage*; D005285:Fermentation; D006801:Humans; D007004:Hypoglycemic Agents / Q000008:administration & dosage*; D008297:Male; D000068219:Prunus armeniaca / Q000737:chemistry / Q000382:microbiology*; D051381:Rats; D017208:Rats, Wistar; D014242:Trichoderma / Q000378:metabolism*; D014866:Waste Products / Q000032:analysis*; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D004043:Dietary Fiber; D007004:Hypoglycemic Agents; D014866:Waste Products; D000520:alpha-Glucosidases