title: Adhesion of adipose-derived mesenchymal stem cells to glycosaminoglycan surfaces with different protein patterns.
abstract: Proteins and glycosaminoglycans (GAGs) are the main constituents of the extracellular matrix (ECM). They act in synergism and are equally critical for the development, growth, function, or survival of an organism. In this work, we developed surfaces that display these two classes of biomacromolecules, namely, GAGs and proteins, in a spatially controlled fashion. The generated surfaces can be used as a minimalistic but straightforward model aiding the elucidation of cell-ECM interactions. GAGs (hyaluronic acid and heparin) were covalently bound to amino functionalized surfaces, and albumin or fibronectin was patterned by microcontact printing on top of them. We demonstrate that adipose-derived stem cells (ASCs) can adhere either on the protein or on the GAG pattern as a function of the patterned molecules. ASCs found on the GAG pattern had different morphology and expressed different surface markers than the cells adhered on the protein pattern. ASCs morphology and spreading were also dependent on the size of the pattern. These results show that the developed supports can also be used for ASCs differentiation into different lineages.
authors.affiliation: †3B's Research Group, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Taipas, Guimarães, Portugal.|‡ICVS/3B's - PT Government Associate Laboratory, Braga, Guimarães, Portugal.
authors.forename: Diana
authors.initials: D
authors.lastname: Soares da Costa
authors.affiliation: §IK4-Tekniker, Micro and Nano Manufacture Unit, Polo Tecnológico De Eibar, C/Iñaki Goenaga 5, 20600 Eibar, Gipuzkoa Spain.|∥CIC microGUNE, Polo de Innovación Garaia, Goiru kalea 9, 20500 Arrasate-Mondragón, Gipuzkoa Spain.
authors.forename: Maria del Carmen
authors.initials: Mdel C
authors.lastname: Márquez-Posadas
authors.affiliation: †3B's Research Group, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Taipas, Guimarães, Portugal.|‡ICVS/3B's - PT Government Associate Laboratory, Braga, Guimarães, Portugal.
authors.forename: Ana R
authors.initials: AR
authors.lastname: Araujo
authors.affiliation: ⊥Biomedical Materials Group, Martin Luther University, Heinrich-Damerow-Strasse 4, 06120 Halle (Saale), Saxony-Anhalt, Germany.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: §IK4-Tekniker, Micro and Nano Manufacture Unit, Polo Tecnológico De Eibar, C/Iñaki Goenaga 5, 20600 Eibar, Gipuzkoa Spain.|∥CIC microGUNE, Polo de Innovación Garaia, Goiru kalea 9, 20500 Arrasate-Mondragón, Gipuzkoa Spain.
authors.forename: Santos
authors.initials: S
authors.lastname: Merino
authors.affiliation: ⊥Biomedical Materials Group, Martin Luther University, Heinrich-Damerow-Strasse 4, 06120 Halle (Saale), Saxony-Anhalt, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Groth
authors.affiliation: †3B's Research Group, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Taipas, Guimarães, Portugal.|‡ICVS/3B's - PT Government Associate Laboratory, Braga, Guimarães, Portugal.
authors.forename: Rui L
authors.initials: RL
authors.lastname: Reis
authors.affiliation: †3B's Research Group, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Taipas, Guimarães, Portugal.|‡ICVS/3B's - PT Government Associate Laboratory, Braga, Guimarães, Portugal.
authors.forename: Iva
authors.initials: I
authors.lastname: Pashkuleva
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D005353:Fibronectins / Q000494:pharmacology; D006025:Glycosaminoglycans / Q000494:pharmacology*; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D010084:Oxidation-Reduction / Q000187:drug effects; D011506:Proteins / Q000494:pharmacology*; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013499:Surface Properties; D055815:Young Adult
chemical_list: D005353:Fibronectins; D006025:Glycosaminoglycans; D018960:Hyaluronan Receptors; D011506:Proteins; D012710:Serum Albumin, Bovine
keywords: CD44; adipose-derived stem cells; glycosaminoglycans; micropatterning