title: Comparative Efficacy of Ceftaroline with Linezolid against Staphylococcus aureus and Methicillin Resistant Staphylococcus aureus.
abstract: OBJECTIVE
  To compare the in vitro antimicrobial efficacy of ceftaroline with linezolid against Staphylococcus aureus and methicillin resistant Staphylococcus aureus.
  
  
  METHODS
  Quasi-experimental study.
  
  
  METHODS
  Microbiology Department, Army Medical College, Rawalpindi, from January to December 2013.
  
  
  METHODS
  Clinical samples from respiratory tract, blood, pus and various catheter tips routinely received in the Department of Microbiology, Army Medical College, Rawalpindi were innoculated on blood and MacConkey agar. Staphylococcus aureus was identified by colony morphology, Gram reaction, catalase test and coagulase test. Methicillin resistant Staphylococcus aureus detection was done by modified Kirby Bauer disc diffusion method using cefoxitin disc (30 μg) and the isolates were considered methicillin resistant if the zone of inhibition around cefoxitin disc was ≤ 21 mm. Bacterial suspensions of 56 Staphylococcus aureus isolates and 50 MRSA isolates were prepared, which were standardized equal to 0.5 McFarland's turbidity standard and inoculated on Mueller-Hinton agar plates followed by application of ceftaroline and linezolid disc (Oxoid, UK), according to manufacturer's instructions. The plates were then incubated at 37 °C aerobically for 18 - 24 hours. Diameters of inhibition zone were measured and interpretated as per Clinical and Laboratory Standards Institute (CLSI) guidelines.
  
  
  RESULTS
  Out of 106 isolates all of the 56 Staphylococcus aureus (100%) were sensitive to ceftaroline and linezolid. However, out of 50 methicillin resistant Staphylococcus aureus, 48 (96%) were sensitive to ceftaroline whereas, 49 (98%) were sensitive to linezolid.
  
  
  CONCLUSIONS
  Ceftaroline is equally effective as linezolid against Staphylococcus aureus and methicillin resistant Staphylococcus aureus.
authors.affiliation: Department of Microbiology, Army Medical College-National University of Science and Technology, Islamabad.
authors.forename: Amira
authors.initials: A
authors.lastname: Hafeez
authors.affiliation: Department of Microbiology, Army Medical College-National University of Science and Technology, Islamabad.
authors.forename: Tehmina
authors.initials: T
authors.lastname: Munir
authors.affiliation: Department of Microbiology, Army Medical College-National University of Science and Technology, Islamabad.
authors.forename: Sabahat
authors.initials: S
authors.lastname: Rehman
authors.affiliation: Department of Microbiology, Army Medical College-National University of Science and Technology, Islamabad.
authors.forename: Sara
authors.initials: S
authors.lastname: Najeeb
authors.affiliation: Department of Microbiology, Army Medical College-National University of Science and Technology, Islamabad.
authors.forename: Mehreen
authors.initials: M
authors.lastname: Gilani
authors.affiliation: Department of Microbiology, Army Medical College-National University of Science and Technology, Islamabad.
authors.forename: Mahwish
authors.initials: M
authors.lastname: Latif
authors.affiliation: Department of Microbiology, Army Medical College-National University of Science and Technology, Islamabad.
authors.forename: Maliha
authors.initials: M
authors.lastname: Ansari
authors.affiliation: Department of Microbiology, Army Medical College-National University of Science and Technology, Islamabad.
authors.forename: Nadia
authors.initials: N
authors.lastname: Saad
mesh_terms: D000081:Acetamides / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002511:Cephalosporins / Q000494:pharmacology*; D006801:Humans; D000069349:Linezolid; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D023303:Oxazolidinones / Q000494:pharmacology*; D013203:Staphylococcal Infections / Q000188:drug therapy* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects; D000097583:Ceftaroline
chemical_list: D000081:Acetamides; D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D000069349:Linezolid; D023303:Oxazolidinones; D000097583:Ceftaroline