title: Three Different Routes of Inoculation for Experimental Infection with Schmallenberg Virus in Sheep.
abstract: Schmallenberg virus (SBV) is an emerging Orthobunyavirus affecting European domestic ruminants. In this study, three groups of ewes (n = 3) were inoculated with 1 ml of an SBV infectious serum, via the subcutaneous (SC), intradermal (ID) or intranasal (IN) route. The ewes were monitored for 10 days and no clinical signs were reported. IN inoculation failed to generate any detectable RNAemia. SC and ID inoculation induced typical SBV RNAemia and seroconversion upon day 6 post-inoculation in 3/3 and 2/3 sheep, respectively. In all the animals that showed RNAemia, the viral genome could be detected in spleen and mesenteric lymph nodes. Both the SC and ID routes seem suitable to properly reproduce field conditions, as comparable observations were reported regarding RNAemia, seroconversion and viral genome detection in organs.
authors.affiliation: Research Unit of Epidemiology and Risk Analysis Applied to Veterinary Sciences (UREAR-ULg), Fundamental and Applied Research for Animals & Health (FARAH), Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.
authors.forename: L
authors.initials: L
authors.lastname: Martinelle
authors.affiliation: Research Unit of Epidemiology and Risk Analysis Applied to Veterinary Sciences (UREAR-ULg), Fundamental and Applied Research for Animals & Health (FARAH), Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.|Veterinary and Agrochemical Research Centre (CODA-CERVA), Operational Directorate Viral Diseases, Brussels, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Poskin
authors.affiliation: Research Unit of Epidemiology and Risk Analysis Applied to Veterinary Sciences (UREAR-ULg), Fundamental and Applied Research for Animals & Health (FARAH), Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.
authors.forename: F
authors.initials: F
authors.lastname: Dal Pozzo
authors.affiliation: Veterinary and Agrochemical Research Centre (CODA-CERVA), Experimental Centre, Machelen, Belgium.
authors.forename: L
authors.initials: L
authors.lastname: Mostin
authors.affiliation: Veterinary and Agrochemical Research Centre (CODA-CERVA), Experimental Centre, Machelen, Belgium.
authors.forename: W
authors.initials: W
authors.lastname: Van Campe
authors.affiliation: Veterinary and Agrochemical Research Centre (CODA-CERVA), Operational Directorate Viral Diseases, Brussels, Belgium.
authors.forename: A B
authors.initials: AB
authors.lastname: Cay
authors.affiliation: Veterinary and Agrochemical Research Centre (CODA-CERVA), Operational Directorate Viral Diseases, Brussels, Belgium.
authors.forename: N
authors.initials: N
authors.lastname: De Regge
authors.affiliation: Research Unit of Epidemiology and Risk Analysis Applied to Veterinary Sciences (UREAR-ULg), Fundamental and Applied Research for Animals & Health (FARAH), Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.
authors.forename: C
authors.initials: C
authors.lastname: Saegerman
mesh_terms: D000281:Administration, Intranasal / Q000662:veterinary; D000818:Animals; D002044:Bunyaviridae Infections / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D005260:Female; D007271:Injections, Intradermal / Q000662:veterinary; D007279:Injections, Subcutaneous / Q000662:veterinary; D008198:Lymph Nodes / Q000821:virology; D016851:Orthobunyavirus / Q000502:physiology*; D012756:Sheep; D012757:Sheep Diseases / Q000517:prevention & control* / Q000821:virology; D013154:Spleen / Q000821:virology; D014611:Vaccination / Q000662:veterinary*
keywords: Schmallenberg; inoculation; route; sheep; virus