title: Monoclonal Antibodies Against the Human Respiratory Syncytial Virus Obtained by Immunization with Epitope Peptides and CpG-DNA-liposome Complex.
abstract: Respiratory syncytial virus (RSV) is the major cause of pulmonary inflammation in infants, young children, and immunocompromised adults. However, the RSV vaccine is not yet available commercially. The RSV-F glycoprotein mediates virus-host cell fusion, leading to syncytial formation; therefore, the RSV-F glycoprotein has been a treatment target for prevention and therapy of RSV infection. To produce the RSV-F-protein epitope-specific monoclonal antibody (MAb), BALB/c mice were immunized with a complex consisting of epitope peptide and MB-ODN 4531(O), encapsulated in a phosphatidyl-β-oleoyl-γ-palmitoyl ethanolamine (DOPE):cholesterol hemisuccinate (CHEMS) complex (Lipoplex(O)). Using conventional hybridoma technology, we obtained two clones able to produce antibodies reactive to two B-cell epitopes of RSV-F protein. Each anti-RSV-F glycoprotein MAb efficiently binds to each epitope. The F7-1A9D10 clone showed specific binding with RSV-F protein. There was no specific protein detected by Western blot analysis using F9 epitope-specific anti-RSV-F glycoprotein MAb (clone F9-1A6C8). However, based on confocal-image analysis, the antibody from the F9-1A6C8 clone showed specific binding with RSV-F protein. It is important that further study on possible applications for passive immunotherapy against RSV infection, such as therapeutic antibody production, is carried out.
authors.affiliation: 1 Department of Microbiology, Hallym University , Chuncheon, Republic of Korea.
authors.forename: Byoung Kwon
authors.initials: BK
authors.lastname: Park
authors.forename: Song Hee
authors.initials: SH
authors.lastname: Choi
authors.forename: Young-Eun
authors.initials: YE
authors.lastname: Kim
authors.forename: Sangkyu
authors.initials: S
authors.lastname: Park
authors.forename: Younghee
authors.initials: Y
authors.lastname: Lee
authors.forename: Keun-Wook
authors.initials: KW
authors.lastname: Lee
authors.forename: Hyung-Joo
authors.initials: HJ
authors.lastname: Kwon
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D004269:DNA, Bacterial / Q000494:pharmacology*; D000939:Epitopes / Q000276:immunology; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D006825:Hybridomas; D007114:Immunization; D008081:Liposomes; D008807:Mice, Inbred BALB C; D009163:Mycobacterium bovis / Q000235:genetics; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology; D010446:Peptide Fragments / Q000276:immunology*; D011485:Protein Binding; D018113:Respiratory Syncytial Virus, Human / Q000276:immunology*; D014709:Vero Cells
chemical_list: D000276:Adjuvants, Immunologic; D058846:Antibodies, Monoclonal, Murine-Derived; D000914:Antibodies, Viral; D004269:DNA, Bacterial; D000939:Epitopes; D008081:Liposomes; D009838:Oligodeoxyribonucleotides; D010446:Peptide Fragments