title: Altered MicroRNA Expression Profiles in Activated Mast Cells Following IgE-FcεRI Cross-Linking with Antigen.
abstract: OBJECTIVE
  MicroRNAs (miRNAs) are critical regulators of immune responses and immunologic disorders. However, little is known about miRNA expression and function during mast cell differentiation, proliferation and activation. This study aimed to determine the miRNA expression profiles in mast cells stimulated by immunoglobulin E (IgE) and antigen and to analyze the potential functions of specific miRNAs.
  
  
  METHODS
  Bone marrow-derived mast cells (BMMCs) generated from differentiated mouse bone marrow cells were untreated (Unstimu) or stimulated with IgE-antigen complexes for 1 h or 6 h (Stimu). The miRNA profiles were evaluated by miRNA microarray. MiRNA target gene prediction and enrichment analyses were performed using bioinformatics.
  
  
  RESULTS
  Seven significantly up-regulated and 10 down-regulated miRNAs were identified in the 1 h Stimu group relative to the Unstimu group (fold change>2; P<0.05). Of 8 miRNAs randomly selected from the 17 identified, the expression levels of 6 were confirmed by quantitative real-time PCR (qRT-PCR). The potential target genes of several candidate miRNAs were enriched in FcεRI signaling, response to stimulus and cellular exocytosis.
  
  
  CONCLUSIONS
  The expression of many miRNAs changes following IgE-FcεRI cross-linking in activated mast cells, and these miRNAs probably play key regulatory roles in core signaling pathways and biological behaviors. Evaluating the functions of these characteristic miRNAs will further our understanding of IgE-associated allergic disease pathogenesis and the development of therapeutic strategies.
authors.affiliation: Department of Otolaryngology, Huadong Hospital, Fudan University, Shanghai, China.
authors.forename: Yaoshu
authors.initials: Y
authors.lastname: Teng
authors.forename: Ruxin
authors.initials: R
authors.lastname: Zhang
authors.forename: Hongzhi
authors.initials: H
authors.lastname: Yu
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Zhicong
authors.initials: Z
authors.lastname: Hong
authors.forename: Wenjie
authors.initials: W
authors.lastname: Zhuang
authors.forename: Yu
authors.initials: Y
authors.lastname: Huang
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology*; D001854:Bone Marrow Cells / Q000276:immunology; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D015536:Down-Regulation / Q000235:genetics / Q000276:immunology; D005089:Exocytosis / Q000235:genetics / Q000276:immunology; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation / Q000235:genetics / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology*; D008297:Male; D008407:Mast Cells / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000235:genetics* / Q000276:immunology; D017455:Receptors, IgE / Q000276:immunology*; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology; D059467:Transcriptome / Q000235:genetics* / Q000276:immunology; D015854:Up-Regulation / Q000235:genetics / Q000276:immunology
chemical_list: D000941:Antigens; D035683:MicroRNAs; D017455:Receptors, IgE; D007073:Immunoglobulin E