title: Expression Analysis and Binding Assays in the Chemosensory Protein Gene Family Indicate Multiple Roles in Helicoverpa armigera.
abstract: Chemosensory proteins (CSPs) have been proposed to capture and transport hydrophobic chemicals to receptors on sensory neurons. We identified and cloned 24 CSP genes to better understand the physiological function of CSPs in Helicoverpa armigera. Quantitative real-time polymerase chain reaction assays indicate that CSP genes are ubiquitously expressed in adult H. armigera tissues. Broad expression patterns in adult tissues suggest that CSPs are involved in a diverse range of cellular processes, including chemosensation as well as other functions not related to chemosensation. The H. armigera CSPs that were highly transcribed in sensory organs or pheromone glands (HarmCSPs 6, 9, 18, 19), were recombinantly expressed in bacteria to explore their function. Fluorescent competitive binding assays were used to measure the binding affinities of these CSPs against 85 plant volatiles and 4 pheromone components. HarmCSP6 displays high binding affinity for pheromone components, whereas the other three proteins do not show affinities for any of the compounds tested. HarmCSP6 is expressed in numerous cells located in or close to long sensilla trichodea on the antennae of both males and females. These results suggest that HarmCSP6 may be involved in transporting female sex pheromones in H. armigera.
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan, China.
authors.forename: Zhao-Qun
authors.initials: ZQ
authors.lastname: Li
authors.forename: Shuai
authors.initials: S
authors.lastname: Zhang
authors.forename: Jun-Yu
authors.initials: JY
authors.lastname: Luo
authors.forename: Jing
authors.initials: J
authors.lastname: Zhu
authors.forename: Jin-Jie
authors.initials: JJ
authors.lastname: Cui
authors.forename: Shuang-Lin
authors.initials: SL
authors.lastname: Dong
mesh_terms: D000818:Animals; D057506:Arthropod Antennae / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D017403:In Situ Hybridization; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D008024:Ligands; D008297:Male; D008961:Models, Structural; D009036:Moths / Q000235:genetics / Q000378:metabolism*; D005810:Multigene Family; D010802:Phylogeny; D011487:Protein Conformation; D018035:Receptors, Odorant / Q000235:genetics / Q000378:metabolism*; D058618:Sensilla / Q000378:metabolism*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D019476:Insect Proteins; D008024:Ligands; D018035:Receptors, Odorant