title: [Immunohistochemical assay of murine liver tissue using monoclonal antibodies to NO-synthase 2 and TNF-α under tetrachloromethane toxic injury conditions].
abstract: Experimental data showing a significant role of enzyme NO-synthase type 2 and cytokine TNF-α enzymes in pathogenesis of CCl4-induced liver injury have been obtained by immunohistochemical assay. It is established that the hepatotoxic agent leads to an increase in NO-synthase 2 and TNF-α levels. The use of both studied phytoadaptogens (eleutherococcus extract and ginseng tincture) at a doses of 48.5 mg/kg and 47 mg/kg, respectively, and the reference drug karsil (100 mg/kg daily for 5 weeks) prevented to a significant degree the accumulation of enzyme NO-synthase 2 and the cytokine TNF-α, which is apparently one of the mechanisms of hepatoprotective action.
authors.forename: I G
authors.initials: IG
authors.lastname: Pechenkina
authors.forename: S V
authors.initials: SV
authors.lastname: Kozin
authors.forename: D V
authors.initials: DV
authors.lastname: Bulanov
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D002252:Carbon Tetrachloride Poisoning / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D028001:Eleutherococcus / Q000737:chemistry; D015870:Gene Expression / Q000187:drug effects; D007150:Immunohistochemistry; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D012838:Silymarin / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D010936:Plant Extracts; D020011:Protective Agents; D012838:Silymarin; D014409:Tumor Necrosis Factor-alpha; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse