title: Revision of the species complex Amidostomum acutum (Lundahl, 1848) (Nematoda: Amidostomatidae) by use of molecular techniques.
abstract: The aim of the work is to confirm the species differentiation of the nematodes of the Amidostomatidae family: Amidostomoides acutum (Lundahl, 1848) Lomakin, 1991; Amidostomoides monodon (Linstow, 1882) Lomakin, 1991, and Amidostomoides petrovi (Shakhtahtinskaya, 1956) Lomakin, 1991, which still are used in the parasitological literature as synonyms of Amidostomum acutum (Lundahl, 1848). The research material consisted of nematodes isolated from gizzards of dabbling ducks from the north-west of Poland. To confirm the species differentiation, DNA from the nematodes was isolated and approximately 630bp of the 28S rRNA gene were sequenced. The obtained DNA sequences were tabulated and then phylogenetic analysis were conducted using the UPGMA method. The results of the research distinctly diversify the nematodes of the genus Amidostomoides at the DNA level, which together with morphological and ecological differences among them (hosts from different systematic groups) enables to classify them into the separate species.
authors.affiliation: Laboratory of Biology and Ecology of Parasites, West Pomeranian University of Technology, Judyma 20, 71-795 Szczecin.
authors.forename: Katarzyna M
authors.initials: KM
authors.lastname: Kavetska
authors.affiliation: Department of Genetics and Animal Breeding, West Pomeranian University of Technology, Al. Piastów 45, 70-311 Szczecin.
authors.forename: Daniel
authors.initials: D
authors.lastname: Polasik
authors.affiliation: Laboratory of Biology and Ecology of Parasites, West Pomeranian University of Technology, Judyma 20, 71-795 Szczecin.
authors.forename: Emil
authors.initials: E
authors.lastname: Dzierzba
authors.affiliation: Laboratory of Biology and Ecology of Parasites, West Pomeranian University of Technology, Judyma 20, 71-795 Szczecin.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Jędrzejczak
authors.affiliation: Department of Biology and Medical Parasitology, Pomeranian Medical University, Powstańców Wielkopolskich 72, 70-111 Szczecin.
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Kalisińska
authors.affiliation: Department of Ecology and Environmental Protection, University of Szczecin, Wąska 13, 75-415 Szczecin; Poland.
authors.forename: Izabella
authors.initials: I
authors.lastname: Rząd
mesh_terms: D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D009348:Nematoda / Q000145:classification* / Q000235:genetics*; D010802:Phylogeny; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D013045:Species Specificity
chemical_list: D012339:RNA, Ribosomal, 28S; D004247:DNA