title: [Determination of phenol in water by electrochemical tyrosinase biosensor based on ordered graphitized mesoporous carbon and evaluated by high performance liquid chromatography].
abstract: A novel electrochemical tyrosinase biosensor based on ordered graphitized mesoporous carbon (GMC) was obtained, which was used as a platform for phenol detection. The accuracy of tyrosinase biosensor method was comparatively evaluated by high performance liquid chromatography (HPLC). By entrapping tyrosinase molecules (6.5 nm x 9.8 nm x 5.5 nm) into the mesopores of GMC (diameter 10 nm, GMC10), the "interspace confinement effect" of GMC10 may improve the stability of tyrosinase in vitro. After 21-day storage, the GMC10-based tyrosinase biosensor retained more than 85% of its initial response. It is indicated that GMC10 with "interspace confinement effect" can significantly prolong the life of tyrosinase molecules in vitro. Furthermore, the GMC-based tyrosinase biosensor displayed excellent analytical performances for phenol detection, such as stability, repeatability, selectivity, sensitivity and limit of detection. The GMC-based tyrosinase biosensor demonstrated a linear response for phenol from 0. 1 to 10 µmol/L with a low detection limit of 20 nmol/L. The comparative study between HPLC and GMC-based tyrosinase biosensor showed that the detection of phenol in water sample by the GMC-based tyrosinase biosensor method is reliable, accurate and effective. The proposed GMC-based tyrosinase biosensor proved to be a very promising "pre-alarm" tool for rapid detecting phenol pollution in emergency accidents.
authors.forename: Lidong
authors.initials: L
authors.lastname: Wu
authors.forename: Huan
authors.initials: H
authors.lastname: Liu
authors.forename: Jincheng
authors.initials: J
authors.lastname: Li
authors.forename: Xiaochen
authors.initials: X
authors.lastname: Fu
authors.forename: Yi
authors.initials: Y
authors.lastname: Song
mesh_terms: D015374:Biosensing Techniques; D002244:Carbon; D002851:Chromatography, High Pressure Liquid; D057230:Limit of Detection; D014442:Monophenol Monooxygenase; D010636:Phenols / Q000032:analysis*; D014867:Water / Q000032:analysis*
chemical_list: D010636:Phenols; D014867:Water; D002244:Carbon; D014442:Monophenol Monooxygenase