title: HNF1B polymorphism influences the prognosis of endometrial cancer patients: a cohort study.
abstract: BACKGROUND
  HNF1B (formerly known as TCF2) gene encodes for a transcription factor that regulates gene expression involved in normal mesodermal and endodermal developments. A close association between rs4430796 polymorphism of HNF1B gene and decreased endometrial cancer (EC) risk has been demonstrated. The aim of the current study was to test the hypothesis that rs4430796 polymorphism can influence the prognosis of EC patients.
  
  
  METHODS
  Retrospective cohort study. Clinical and pathological data were extrapolated and genotypes were assessed on formalin-fixed and paraffin-embedded non-tumour tissues. The influence of patients' genotype on overall survival and progression free survival were our main outcome measures.
  
  
  RESULTS
  A total of 191 EC patients were included in the final analysis. Overall survival differed significantly (P = 0.003) among genotypes. At multivariate analysis, a significant (P < 0.05) effect on overall survival was detected for FIGO stage, and rs4430796 polymorphism of HNF1B gene. After grouping EC patients according to adjuvant treatment, rs4430796 polymorphism resulted significantly (P < 0.001) related to overall survival only in subjects who received radiotherapy plus chemotherapy. A significant (P = 0.014) interaction between rs4430796 polymorphism and chemo-radiotherapy was also detected. Finally, only a trend (P = 0.090) towards significance was observed for rs4430796 polymorphism effect on progression free survival.
  
  
  CONCLUSIONS
  rs4430796 polymorphism of HNF1B gene influences independently the prognosis of EC patients with a potential effect on tumor chemo-sensitivity.
authors.affiliation: Unit of Obstetrics and Gynecology, IRCCS-Arcispedale Santa Maria Nuova, Reggio Emilia, Italy. dariomandato@virgilio.it.
authors.forename: Vincenzo Dario
authors.initials: VD
authors.lastname: Mandato
authors.affiliation: Laboratory of Molecular Biology, IRCCS-Arcispedale Santa Maria Nuova, Reggio Emilia, Italy. enrico.farnetti@asmn.re.it.
authors.forename: Enrico
authors.initials: E
authors.lastname: Farnetti
authors.affiliation: Laboratory of Molecular Biology, IRCCS-Arcispedale Santa Maria Nuova, Reggio Emilia, Italy. federica.torricelli@asmn.re.it.
authors.forename: Federica
authors.initials: F
authors.lastname: Torricelli
authors.affiliation: Unit of Obstetrics and Gynecology, IRCCS-Arcispedale Santa Maria Nuova, Reggio Emilia, Italy. martino.abrate@asmn.re.it.
authors.forename: Martino
authors.initials: M
authors.lastname: Abrate
authors.affiliation: Laboratory of Molecular Biology, IRCCS-Arcispedale Santa Maria Nuova, Reggio Emilia, Italy. bruno.casali@asmn.re.it.
authors.forename: Bruno
authors.initials: B
authors.lastname: Casali
authors.affiliation: Unit of Obstetrics and Gynecology, IRCCS-Arcispedale Santa Maria Nuova, Reggio Emilia, Italy. gino.ciarlini@asmn.re.it.
authors.forename: Gino
authors.initials: G
authors.lastname: Ciarlini
authors.affiliation: Unit of Obstetrics and Gynecology, IRCCS-Arcispedale Santa Maria Nuova, Reggio Emilia, Italy. debora.pirillo@asmn.re.it.
authors.forename: Debora
authors.initials: D
authors.lastname: Pirillo
authors.affiliation: Unit of Pathology, IRCCS-Arcispedale Santa Maria Nuova, Reggio Emilia, Italy. mariacarolina.gelli@asmn.re.it.
authors.forename: Maria Carolina
authors.initials: MC
authors.lastname: Gelli
authors.affiliation: Laboratory of Molecular Biology, IRCCS-Arcispedale Santa Maria Nuova, Reggio Emilia, Italy. davide.nicoli@asmn.re.it.
authors.forename: Davide
authors.initials: D
authors.lastname: Nicoli
authors.affiliation: Department of Brain and Behavioral Science, Medical and Genomics Statistics Unit, University of Pavia, Pavia, Italy. mario.grassi@unipv.it.
authors.forename: Mario
authors.initials: M
authors.lastname: Grassi
authors.affiliation: Unit of Obstetrics and Gynecology, IRCCS-Arcispedale Santa Maria Nuova, Reggio Emilia, Italy. giovannibattista.lasala@asmn.re.it.|University of Modena and Reggio Emilia, Modena, Italy. giovannibattista.lasala@asmn.re.it.
authors.forename: Giovanni Battista
authors.initials: GB
authors.lastname: LA Sala
authors.affiliation: Unit of Gynecologic Oncology, IRCCS-CROB, Rionero in Vulture (Potenza), Potenza, Italy. stefanopalomba@tin.it.
authors.forename: Stefano
authors.initials: S
authors.lastname: Palomba
mesh_terms: D000368:Aged; D000483:Alleles; D059186:Chemoradiotherapy, Adjuvant; D015331:Cohort Studies; D018450:Disease Progression; D016889:Endometrial Neoplasms / Q000235:genetics* / Q000401:mortality* / Q000473:pathology / Q000628:therapy; D005260:Female; D005500:Follow-Up Studies; D005838:Genotype; D051539:Hepatocyte Nuclear Factor 1-beta / Q000235:genetics*; D006801:Humans; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D016016:Proportional Hazards Models; D012189:Retrospective Studies
chemical_list: C496495:HNF1B protein, human; D051539:Hepatocyte Nuclear Factor 1-beta