title: Long non-coding RNA HOTAIR promotes tumor cell invasion and metastasis by recruiting EZH2 and repressing E-cadherin in oral squamous cell carcinoma.
abstract: HOX transcript antisense RNA (HOTAIR), a long intergenic non-coding RNA (lncRNA), functions as a molecular scaffold to link and target the histone modification complexes PRC2 and LSD1, then reprograms chromatin states by coupling histone H3K27 methylation and H3K4 demethylation for epigenetic gene silencing to promote cancer metastasis. It is associated with poor survival in several solid cancers. In this study, we show that HOTAIR expression increased in oral squamous cell carcinoma (OSCC) compared with non-tumor tissue and is associated with metastasis, the stage and histological differentiation. In addition, overexpression of HOTAIR indicated poor overall survival (OS) and disease-free survival (DFS) in OSCC patients. Knockdown of HOTAIR by siRNA in OSCC cells decreased cell proliferation and colony formation, increased cell invasion and migration, and induced apoptosis in vitro. Furthermore, significant negative correlation between HOTAIR levels and E-cadherin levels was found in OSCC tissues and cell lines, and HOTAIR contributed to the regulation of E-cadherin through binding to EZH2 and H3K27me3 with the E-cadherin promoter. Our findings suggest that HOTAIR expression is associated with OSCC and may be one of critical targets in progression and metastasis, and an indicator of poor survival in OSCC.
authors.affiliation: Department of Maxillofacial and Otorhinolaryngology Head and Neck Surgery, Tianjin Medical University Cancer Institute and Hospital, Tianjin, P.R. China.
authors.forename: Yansheng
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Breast Cancer Medical Oncology, Tianjin Medical University Cancer Institute and Hospital, Tianjin, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Maxillofacial and Otorhinolaryngology Head and Neck Surgery, Tianjin Medical University Cancer Institute and Hospital, Tianjin, P.R. China.
authors.forename: Lun
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Biotherapy Center, Tianjin Medical University Cancer Institute and Hospital, Tianjin, P.R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Biotherapy Center, Tianjin Medical University Cancer Institute and Hospital, Tianjin, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Immunology, Tianjin Medical University Cancer Institute and Hospital, Tianjin, P.R. China.
authors.forename: Xiubao
authors.initials: X
authors.lastname: Ren
authors.affiliation: Biotherapy Center, Tianjin Medical University Cancer Institute and Hospital, Tianjin, P.R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wei
authors.affiliation: Biotherapy Center, Tianjin Medical University Cancer Institute and Hospital, Tianjin, P.R. China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Yu
authors.affiliation: Biotherapy Center, Tianjin Medical University Cancer Institute and Hospital, Tianjin, P.R. China.
authors.forename: Ting
authors.initials: T
authors.lastname: Liu
authors.affiliation: Department of Maxillofacial and Otorhinolaryngology Head and Neck Surgery, Tianjin Medical University Cancer Institute and Hospital, Tianjin, P.R. China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Maxillofacial and Otorhinolaryngology Head and Neck Surgery, Tianjin Medical University Cancer Institute and Hospital, Tianjin, P.R. China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Biotherapy Center, Tianjin Medical University Cancer Institute and Hospital, Tianjin, P.R. China.
authors.forename: Jinpu
authors.initials: J
authors.lastname: Yu
authors.affiliation: Biotherapy Center, Tianjin Medical University Cancer Institute and Hospital, Tianjin, P.R. China.
authors.forename: Xishan
authors.initials: X
authors.lastname: Hao
mesh_terms: D000328:Adult; D000368:Aged; D015820:Cadherins / Q000235:genetics* / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D000071221:Enhancer of Zeste Homolog 2 Protein; D044127:Epigenesis, Genetic; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D063151:Polycomb Repressive Complex 2 / Q000235:genetics* / Q000378:metabolism; D011379:Prognosis; D062085:RNA, Long Noncoding / Q000235:genetics*
chemical_list: D015820:Cadherins; C561566:HOTAIR long untranslated RNA, human; D062085:RNA, Long Noncoding; C498409:EZH2 protein, human; D000071221:Enhancer of Zeste Homolog 2 Protein; D063151:Polycomb Repressive Complex 2