title: Detection of morphologic alterations in rectal carcinoma following preoperative radiochemotherapy based on multiphoton microscopy imaging.
abstract: BACKGROUND
  Preoperative radiochemotherapy improves outcomes in patients with locally advanced rectal carcinoma, and has been used increasingly in patient management. However, there is a strong clinical need to assess tumor response to neoadjuvant treatment, and a non-invasive technique that allows the precise identification of morphologic changes in tumors would be of considerable clinical interest.
  
  
  METHODS
  In this study, we used multiphoton microscopy (MPM) to detect morphologic alterations in rectal adenocarcinomas in patients treated with preoperative radiochemotherapy.
  
  
  RESULTS
  MPM was able to identify histopathologic alterations in rectal cancer following preoperative radiochemotherapy, and allowed the qualitative assessment of treatment efficacy and feasibility in relation to dose or strategy.
  
  
  CONCLUSIONS
  These findings may provide the groundwork for evaluating tumor response to neoadjuvant treatment, thus allowing the tailoring of effective treatment doses and strategies.
authors.affiliation: Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, 350007, China. lhli@fjnu.edu.cn.
authors.forename: Lianhuang
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Colorectal Surgery, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, 350001, China. chenzhifen@gmail.com.
authors.forename: Zhifen
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Pathology, The First Affiliated Hospital, Fujian Medical University, Fuzhou, 350001, China. wang_xfu@126.com.
authors.forename: Xingfu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, 350007, China. lihongsheng0520@163.com.
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Colorectal Surgery, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, 350001, China. jiangwz362100@163.com.
authors.forename: Weizhong
authors.initials: W
authors.lastname: Jiang
authors.affiliation: Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, 350007, China. smzhuo@fjnu.edu.cn.
authors.forename: Shuangmu
authors.initials: S
authors.lastname: Zhuo
authors.affiliation: Department of Colorectal Surgery, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, 350001, China. gxguan1108@163.com.
authors.forename: Guoxian
authors.initials: G
authors.lastname: Guan
authors.affiliation: Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, 350007, China. chenjianxin@fjnu.edu.cn.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Chen
mesh_terms: D000328:Adult; D000368:Aged; D059248:Chemoradiotherapy / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D008875:Middle Aged; D011300:Preoperative Care / Q000379:methods*; D012004:Rectal Neoplasms / Q000175:diagnosis* / Q000628:therapy*; D016896:Treatment Outcome