title: The motorized RhoGAP myosin IXb (Myo9b) in leukocytes regulates experimental autoimmune encephalomyelitis induction and recovery.
abstract: Myo9b regulates leukocyte migration by controlling RhoA signaling. Here we assessed its role in active experimental autoimmune encephalomyelitis (EAE). Myo9b(-/-) mice show a delay in the onset of EAE symptoms. The delay in disease onset was accompanied by reduced numbers of Th1 and Th17 cells in the CNS. Myo9b(-/-) mice showed no recovery from disease symptoms and exhibited elevated numbers of both Th17 cells and CD11b+ macrophages. Bone marrow chimeric mice demonstrated that the absence of a leukocyte source of Myo9b was responsible for the delayed leukocyte infiltration into the CNS, delayed EAE onset and lack of recovery.
authors.affiliation: Institute of Molecular Cell Biology, Westfalian Wilhelms-University Münster, Germany.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Institute of Molecular Cell Biology, Westfalian Wilhelms-University Münster, Germany.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Institute of Physiological Chemistry and Pathobiochemistry, Westfalian Wilhelms-University Münster, Germany.
authors.forename: Xueli
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Institute of Physiological Chemistry and Pathobiochemistry, Westfalian Wilhelms-University Münster, Germany.
authors.forename: Jian
authors.initials: J
authors.lastname: Song
authors.affiliation: Institute of Physiological Chemistry and Pathobiochemistry, Westfalian Wilhelms-University Münster, Germany.
authors.forename: Lydia
authors.initials: L
authors.lastname: Sorokin
authors.affiliation: Institute of Molecular Cell Biology, Westfalian Wilhelms-University Münster, Germany. Electronic address: baehler@uni-muenster.de.
authors.forename: Martin
authors.initials: M
authors.lastname: Bähler
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D049109:Cell Proliferation / Q000235:genetics; D002490:Central Nervous System / Q000378:metabolism / Q000473:pathology; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000139:chemically induced / Q000473:pathology* / Q000503:physiopathology*; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000235:genetics*; D019044:Image Cytometry; D017493:Leukocyte Common Antigens / Q000235:genetics / Q000378:metabolism; D007962:Leukocytes / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D063308:Myelin-Oligodendrocyte Glycoprotein / Q000633:toxicity; D009218:Myosins / Q000235:genetics / Q000378:metabolism*; D010446:Peptide Fragments / Q000633:toxicity; D020127:Recovery of Function / Q000235:genetics*; D013997:Time Factors
chemical_list: D015703:Antigens, CD; D016207:Cytokines; D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments; C445081:myelin oligodendrocyte glycoprotein (35-55); C113469:myosin IXB; D017493:Leukocyte Common Antigens; C516770:Ptprc protein, mouse; D009218:Myosins
keywords: Bone marrow chimeric mice; Clinical features; Experimental autoimmune encephalomyelitis; Myosin IXb; RhoGAP