title: Early-phase peritoneal drainage and lavage in a rat model of severe acute pancreatitis.
abstract: OBJECTIVE
  To evaluate the effects of early-phase drainage on the survival rates and pancreatic pathological changes associated with severe acute pancreatitis (SAP) in a rat model.
  
  
  METHODS
  Sprague-Dawley rats were divided into the following groups: SAP model (control), early drainage and delayed drainage. The 24-h survival rates were compared among the groups. In addition, the serum and ascites concentrations of interleukin (IL)-1β, IL-6, IL-8, IL-10 and tumor necrosis factor (TNF)-α were measured, and pancreatic pathological changes were observed.
  
  
  RESULTS
  The survival rate significantly improved in the early drainage group. Compared with that observed in the control group, the serum TNF-α and IL-8 concentrations in the early drainage group decreased, while the serum IL-10 levels increased, and the ascites concentrations of IL-1β, IL-6, IL-8 and TNF-α decreased, while that of IL-10 increased significantly. In the delayed drainage group, only the ascites concentrations of TNF-α decreased. Meanwhile, the pancreatic pathological changes at 3, 6 and 24 h worsened in the early drainage group; however, the pancreatic lesions in the early drainage group were less mild than those seen in the control group.
  
  
  CONCLUSIONS
  Rebalancing the cytokine levels in ascites after early drainage may be a key factor for enhancing the survival rate in rats.
authors.affiliation: Department of General Surgery, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, 1111 Xianxia Road, Shanghai, 200336, China. zhu1leiming@163.com.
authors.forename: Leiming
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Department of General Surgery, No. 1 Hospital, Shanghai Jiao Tong University School of Medicine, 85 Wujin Road, Shanghai, 200080, China.
authors.forename: Jilin
authors.initials: J
authors.lastname: Lu
authors.affiliation: Department of Pathology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, 1111 Xianxia Road, Shanghai, 200336, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of General Surgery, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, 1111 Xianxia Road, Shanghai, 200336, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Sun
mesh_terms: D000208:Acute Disease; D000818:Animals; D001201:Ascites / Q000378:metabolism; D004195:Disease Models, Animal; D004322:Drainage / Q000379:methods*; D007378:Interleukins / Q000378:metabolism; D008297:Male; D010179:Pancreas / Q000473:pathology; D010195:Pancreatitis / Q000378:metabolism / Q000401:mortality / Q000473:pathology / Q000601:surgery*; D017207:Rats, Sprague-Dawley; D012720:Severity of Illness Index; D015996:Survival Rate; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D007378:Interleukins; D014409:Tumor Necrosis Factor-alpha
keywords: Cytokine; Pathological change; Peritoneal drainage; Severe acute pancreatitis; Survival rate