title: Growth, testis size, spermatogenesis, semen parameters and seminal plasma and sperm membrane protein profile during the reproductive development of male goats supplemented with de-oiled castor cake.
abstract: The present study was conducted to evaluate the effect of de-oiled castor cake on reproductive traits of crossbreed goats. Fourteen males were grouped into two lots (n = 7/group), as described: group without de-oiled castor cake (WCC) and group fed with de-oiled castor cake (CC). Goats received two diets containing a mixture of Bermudagrass hay and concentrates with the same energy (73% total digestive nutrients) and protein content (15% crude protein) during 150 days, corresponding to ages from 40 (puberty) to 60 weeks. Blood plasma concentrations of urea, albumin, lactate dehydrogenase, creatinine, alanine aminotransferase and testosterone were determined. We also evaluated scrotal circumference, sperm parameters, quantitative aspects of spermatogenesis and daily sperm production (DSP), as well as the proteome of seminal plasma and sperm membrane. Seminal fluid and sperm proteins were analyzed by 2D SDS-PAGE and mass spectrometry. After 150 days of castor cake feeding, animals had no changes in the biochemical composition of blood plasma, suggesting the absence of intoxication by ingestion of ricin. There were no alterations in dry mater intake, weight gain, testis size, peripheral concentrations of testosterone, sperm concentration, motility and morphology. Sertoli and germ cell populations in the testis and DSP were not affected either. However, there were significant variations in the expression of five seminal plasma proteins and four sperm membrane proteins. In conclusion, the replacement of soybean meal by castor cake (with ricin concentrations of 50mg/kg) did not interfere with the growth and core reproductive development of male goats. However, the diet with ricin altered the expression of certain seminal plasma and sperm membrane proteins, which play roles in sperm function and fertilization. Lower expression of these proteins may impair the ricin-fed animals to perform as high-fertility sires.
authors.affiliation: Department of Veterinary Medicine, State University of Ceará, CEP 60740-000, Fortaleza, Ceará, Brazil. Electronic address: nine.maia@yahoo.com.br.
authors.forename: C H A
authors.initials: CH
authors.lastname: Oliveira
authors.affiliation: Department of Veterinary Medicine, State University of Ceará, CEP 60740-000, Fortaleza, Ceará, Brazil.
authors.forename: A M
authors.initials: AM
authors.lastname: Silva
authors.affiliation: Department of Veterinary Medicine, State University of Ceará, CEP 60740-000, Fortaleza, Ceará, Brazil.
authors.forename: L M
authors.initials: LM
authors.lastname: Silva
authors.affiliation: Department of Animal Science, Federal University of Ceará, CEP 60356-000, Fortaleza, Ceará, Brazil.
authors.forename: M F
authors.initials: MF
authors.lastname: van Tilburg
authors.affiliation: Department of Veterinary Medicine, State University of Ceará, CEP 60740-000, Fortaleza, Ceará, Brazil.
authors.forename: C C L
authors.initials: CC
authors.lastname: Fernandes
authors.affiliation: Department of Animal Science, Federal University of Ceará, CEP 60356-000, Fortaleza, Ceará, Brazil.
authors.forename: A L M C
authors.initials: AL
authors.lastname: Velho
authors.affiliation: Department of Animal Science, Federal University of Ceará, CEP 60356-000, Fortaleza, Ceará, Brazil.
authors.forename: A A
authors.initials: AA
authors.lastname: Moura
authors.affiliation: Department of Biochemistry, Federal University of Ceará, CEP 60356-000, Fortaleza, Ceará, Brazil.
authors.forename: F B M B
authors.initials: FB
authors.lastname: Moreno
authors.affiliation: Department of Pharmacy, University of Fortaleza, Ceará, CEP 60811-905, Fortaleza, Ceará, Brazil.
authors.forename: A C O
authors.initials: AC
authors.lastname: Monteiro-Moreira
authors.affiliation: Department of Pharmacy, University of Fortaleza, Ceará, CEP 60811-905, Fortaleza, Ceará, Brazil.
authors.forename: R A
authors.initials: RA
authors.lastname: Moreira
authors.affiliation: Mauricio de Nassau Faculty, CEP 60055-402, Fortaleza, Ceará, Brazil.
authors.forename: I M T
authors.initials: IM
authors.lastname: Lima
authors.affiliation: Department of Veterinary Medicine, State University of Ceará, CEP 60740-000, Fortaleza, Ceará, Brazil. Electronic address: claudiohao@hotmail.com.
authors.forename: D
authors.initials: D
authors.lastname: Rondina
mesh_terms: D000818:Animals; D006041:Goats; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D012276:Ricin / Q000633:toxicity*; D012661:Semen / Q000187:drug effects* / Q000378:metabolism; D012741:Sexual Maturation / Q000187:drug effects; D013091:Spermatogenesis / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism; D013737:Testis / Q000166:cytology / Q000187:drug effects; D013739:Testosterone / Q000097:blood
chemical_list: D008565:Membrane Proteins; D013739:Testosterone; D012276:Ricin
keywords: 2D electrophoresis; Caprine; Ricinus communis; Semen quality; Spermatogenesis