title: Probing protein quinary interactions by in-cell nuclear magnetic resonance spectroscopy.
abstract: Historically introduced by McConkey to explain the slow mutation rate of highly abundant proteins, weak protein (quinary) interactions are an emergent property of living cells. The protein complexes that result from quinary interactions are transient and thus difficult to study biochemically in vitro. Cross-correlated relaxation-induced polarization transfer-based in-cell nuclear magnetic resonance allows the characterization of protein quinary interactions with atomic resolution inside live prokaryotic and eukaryotic cells. We show that RNAs are an important component of protein quinary interactions. Protein quinary interactions are unique to the target protein and may affect physicochemical properties, protein activity, and interactions with drugs.
authors.affiliation: Department of Chemistry, University at Albany, State University of New York, Albany, New York 12222, United States.
authors.forename: Subhabrata
authors.initials: S
authors.lastname: Majumder
authors.affiliation: Department of Chemistry, University at Albany, State University of New York, Albany, New York 12222, United States.
authors.forename: Jing
authors.initials: J
authors.lastname: Xue
authors.affiliation: Department of Chemistry, University at Albany, State University of New York, Albany, New York 12222, United States.
authors.forename: Christopher M
authors.initials: CM
authors.lastname: DeMott
authors.affiliation: Department of Chemistry, University at Albany, State University of New York, Albany, New York 12222, United States.
authors.forename: Sergey
authors.initials: S
authors.lastname: Reverdatto
authors.affiliation: Department of Chemistry, University at Albany, State University of New York, Albany, New York 12222, United States.
authors.forename: David S
authors.initials: DS
authors.lastname: Burz
authors.affiliation: Department of Chemistry, University at Albany, State University of New York, Albany, New York 12222, United States.
authors.forename: Alexander
authors.initials: A
authors.lastname: Shekhtman
mesh_terms: D001483:Base Sequence; D015342:DNA Probes; D018274:Electroporation; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008958:Models, Molecular; D011506:Proteins / Q000737:chemistry* / Q000235:genetics; D012313:RNA / Q000737:chemistry; D014162:Transfection
chemical_list: D015342:DNA Probes; D011506:Proteins; D012313:RNA