title: [Expression optimization of Bacillus pumilus subtilisin-like proteinase].
abstract: The characteristics of Bacillus pumilus subtilisin-like protease expression were investigated. Potential binding sites for the transcription factor DegU~P have been identified in the regulatory region of the enzyme gene, which have been optimized for interaction with the regulatory protein. The expression of the extracellular subtilisin-like protease modified gene has been examined. It was established that the modification of one of the sites has resulted in increased expression of the proteinase in 2 times. It is concluded that the optimization of the promoter led to increased expression of subtilisin-like protease.
authors.forename: A M
authors.initials: AM
authors.lastname: Cheremin
authors.forename: Ch
authors.initials: Ch
authors.lastname: Niamsuren
authors.forename: A A
authors.initials: AA
authors.lastname: Toĭmentseva
authors.forename: M R
authors.initials: MR
authors.lastname: Sharipova
mesh_terms: D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology*; D015971:Gene Expression Regulation, Enzymologic; D010447:Peptide Hydrolases / Q000096:biosynthesis* / Q000737:chemistry; D011401:Promoter Regions, Genetic; D012697:Serine Endopeptidases / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism; D013379:Substrate Specificity; D020860:Subtilisin / Q000096:biosynthesis* / Q000737:chemistry
chemical_list: D010447:Peptide Hydrolases; D012697:Serine Endopeptidases; D020860:Subtilisin