title: Optimized hydrolysis and analysis of Radix Asparagi polysaccharide monosaccharide composition by capillary zone electrophoresis.
abstract: Using orthogonal design, optimized conditions for the hydrolysis of the polysaccharide from Radix Asparagi were determined, as well as its monosaccharide composition. Optimized hydrolysis conditions were a temperature of 100°C in 1.5 M sulfuric acid solution for 5 h. The resulting monosaccharides were derivatized with 1-phenyl-3-methyl-5-pyrazolone, then separated by capillary zone electrophoresis in 40 mM sodium tetraborate buffer (pH 10.1), and detected by ultraviolet absorption at 245 nm. Results indicate that the polysaccharide from Radix Asparagi is composed of xylose, arabinose, glucose, rhamnose, mannose, galactose, glucuronic acid, and galacturonic acid, which differs from published findings. Moreover, xylose, glucuronic acid, and galacturonic acid have not been previously reported in Radix Asparagi polysaccharide. This method is simple, fast, and yields a highly efficient separation. As well, these findings can be applied to quality control of Radix Asparagi and for in-depth study of the biological activity of Radix Asparagi polysaccharide.
authors.affiliation: College of Pharmacy, Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Hebei University, Baoding, China.|Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Hebei University, Baoding, China.
authors.forename: Jiye
authors.initials: J
authors.lastname: Chen
authors.affiliation: College of Pharmacy, Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Hebei University, Baoding, China.|Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Hebei University, Baoding, China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Yang
authors.affiliation: College of Pharmacy, Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Hebei University, Baoding, China.|Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Hebei University, Baoding, China.
authors.forename: Huaizhong
authors.initials: H
authors.lastname: Guo
authors.affiliation: College of Pharmacy, Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Hebei University, Baoding, China.|Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Hebei University, Baoding, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wu
authors.affiliation: College of Pharmacy, Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Hebei University, Baoding, China.
authors.forename: Xiaohuan
authors.initials: X
authors.lastname: Wang
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D006868:Hydrolysis; D008516:Medicine, Chinese Traditional; D009005:Monosaccharides / Q000032:analysis*; D011134:Polysaccharides / Q000032:analysis*
chemical_list: D009005:Monosaccharides; D011134:Polysaccharides
keywords: Capillary zone electrophoresis; Hydrolysis; Monosaccharides; Polysaccharides; Radix Asparagi