title: Alteration of gene expression profile following PPP2R5C knockdown may be associated with proliferation suppression and increased apoptosis of K562 cells.
abstract: We reported that knockdown of PPP2R5C by siRNA led to proliferation inhibition and apoptosis induction in K562 cells. In this study, we further characterized the gene expression profiles after PPP2R5C suppression by microarray analysis. Genes which participate in the MAPK, PI3K/AKT, and JAK/STAT pathways, were mainly altered in the K562 cells. We propose that the mechanism for proliferation inhibition and increased apoptosis of K562 cells following PPP2R5C suppression may be related to the alteration of expression profiles of BRAF, AKT2, AKT3, NFKB2 and STAT3 genes.
authors.affiliation: Institute of Hematology, Jinan University, Guangzhou, 510632, China. lsc_csl@163.com.
authors.forename: Sichu
authors.initials: S
authors.lastname: Liu
authors.affiliation: Institute of Hematology, Jinan University, Guangzhou, 510632, China. 25nancy@163.com.|Department of Hematology, The Second Clinical Medical college (Shenzhen People's Hospital), Jinan University, Shenzhen, 518020, China. 25nancy@163.com.
authors.forename: Qi
authors.initials: Q
authors.lastname: Shen
authors.affiliation: Institute of Hematology, Jinan University, Guangzhou, 510632, China. 695401743@qq.com.|Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University, Guangzhou, 510632, China. 695401743@qq.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Institute of Hematology, Jinan University, Guangzhou, 510632, China. bio-zcw@163.com.
authors.forename: Chengwu
authors.initials: C
authors.lastname: Zeng
authors.affiliation: Institute of Hematology, Jinan University, Guangzhou, 510632, China. tcaocs@jnu.edu.cn.
authors.forename: Changshu
authors.initials: C
authors.lastname: Cao
authors.affiliation: Institute of Hematology, Jinan University, Guangzhou, 510632, China. jnyanglijian@163.com.
authors.forename: Lijian
authors.initials: L
authors.lastname: Yang
authors.affiliation: Institute of Hematology, Jinan University, Guangzhou, 510632, China. jnshaohuachen@163.com.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Chen
authors.affiliation: Institute of Hematology, Jinan University, Guangzhou, 510632, China. siulier@163.com.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Wu
authors.affiliation: Institute of Hematology, Jinan University, Guangzhou, 510632, China. libo517@hotmail.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.affiliation: Institute of Hematology, Jinan University, Guangzhou, 510632, China. yangqiuli@hotmail.com.|Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University, Guangzhou, 510632, China. yangqiuli@hotmail.com.
authors.forename: Yangqiu
authors.initials: Y
authors.lastname: Li
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D055785:Gene Knockdown Techniques; D006801:Humans; D020014:K562 Cells; D020411:Oligonucleotide Array Sequence Analysis; D054648:Protein Phosphatase 2 / Q000235:genetics*; D059467:Transcriptome / Q000235:genetics*; D014162:Transfection
chemical_list: C503981:PPP2R5C protein, human; D054648:Protein Phosphatase 2