title: Development of antibacterial quaternary ammonium silane coatings on polyurethane catheters.
abstract: Polyurethene (PU) catheters were coated with 3-(trimethoxysilyl)-propyldimethyloctadecylammonium chloride (QAS) by means of a multistep process which involved a vapor phase plasma-induced graft-polymerization of acrylic acid (AAc). The AAc coating, whose stability in aqueous media was assessed by immersion in Phospate Buffer Saline (PBS), was characterized by means of Attenuated Total Reflectance Fourier Transform Infrared (ATR/FTIR) spectroscopy. Moreover, the COOH surface density was evaluated by a colorimetric assay with Methylene Blue. Carrying a negative charge at neutral pH, AAc coatings were proficient in positively charged molecules (like QAS) adsorption. ATR/FTIR spectroscopy and a colorimetric assay with Bromophenol Blue allowed us to verify the presence and the uniformity of the QAS coating on the PU catheters and the positive effect of the AAc graft-polymerization on the QAS adsorption. Morphological characterization of the QAS-modified catheters was performed by means of Atomic Force Microscopy (AFM). QAS-coated catheters displayed in vitro antimicrobial activity against Gram-negative Escherichia coli bacterial cells.
authors.affiliation: Università degli Studi di Milano-Bicocca, Dipartimento di Fisica "G. Occhialini", p.za della Scienza, 3, I-20126 Milano, Italy. Electronic address: stefano.zanini@mib.infn.it.
authors.forename: Stefano
authors.initials: S
authors.lastname: Zanini
authors.affiliation: Università degli Studi di Milano-Bicocca, Dipartimento di Biotecnologie e Bioscienze, piazza della Scienza 2, I-20126 Milano, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Polissi
authors.affiliation: Università degli Studi di Milano-Bicocca, Dipartimento di Biotecnologie e Bioscienze, piazza della Scienza 2, I-20126 Milano, Italy.
authors.forename: Elisa A
authors.initials: EA
authors.lastname: Maccagni
authors.affiliation: Università degli Studi di Milano-Bicocca, Dipartimento di Fisica "G. Occhialini", p.za della Scienza, 3, I-20126 Milano, Italy.
authors.forename: Elisa C
authors.initials: EC
authors.lastname: Dell'Orto
authors.affiliation: Hilase Centre, Institute of Physics ASCR, v.v.i., Za Radnicí 828, 25241 Dolní Břežany, Czech Republic; Czech Technical University, Thákurova 2077/7, 160 00 Prague 6, Czech Republic.
authors.forename: Chiara
authors.initials: C
authors.lastname: Liberatore
authors.affiliation: Università degli Studi di Milano-Bicocca, Dipartimento di Fisica "G. Occhialini", p.za della Scienza, 3, I-20126 Milano, Italy. Electronic address: claudia.riccardi@mib.infn.it.
authors.forename: Claudia
authors.initials: C
authors.lastname: Riccardi
mesh_terms: D000179:Acrylates / Q000737:chemistry / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D057785:Catheters / Q000382:microbiology*; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects*; D004927:Escherichia coli Infections / Q000517:prevention & control; D006801:Humans; D008826:Microbial Sensitivity Tests; D011140:Polyurethanes / Q000737:chemistry* / Q000494:pharmacology; D000644:Quaternary Ammonium Compounds / Q000737:chemistry* / Q000494:pharmacology; D012821:Silanes / Q000737:chemistry* / Q000494:pharmacology; D013499:Surface Properties
chemical_list: D000179:Acrylates; D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D011140:Polyurethanes; D000644:Quaternary Ammonium Compounds; D012821:Silanes; C036658:acrylic acid
keywords: AFM; ATR/FTIR; Acrylic acid; Escherichia coli; Plasma-induced graft-polymerization; Quaternary ammonium silane