title: Polymorphisms in cyclooxygenase-2 gene in endometrial cancer patients.
abstract: The enzyme cyclooxygenase 2 is an inducible enzyme expressed at sites of inflammation and in a variety of malignant solid tumors such as endometrial cancer (EC). In EC patients, its over-expression is correlated with progressive disease and poor prognosis. The expression is encoded by a polymorphic gene, called PTGS2. The aim of the current study was to test the hypothesis that rs5275 polymorphism of PTGS2 influence the prognosis of EC patients. This paper is a retrospective cohort study. Clinical and pathological data were extrapolated and genotypes were assessed on formalin-fixed and paraffin-embedded non-tumor tissues. A total of 159 type I EC patients were included in the final analysis. Univariate analysis indicated that patients with rs5275 genotype CC have a lower risk to develop a grade (G) 2-3 endometrial cancer. rs5275 effect on EC grading was confirmed by multivariate analysis also after data adjusting for age, BMI, parity, hypertension, and diabetes. Adjusted odds ratio (OR) confirmed that patients with rs5275 genotype CC have a risk 80 % lower (OR = 0.20, P = 0.009) to develop a G2 and/or G3 EC in comparison with patients with TT or TC genotype. Differentiation of the type 1 EC is significantly and independently influenced by rs5275 polymorphism. rs5275 CC patients have a lower risk to present a G2-G3 EC.
authors.affiliation: Laboratory of Molecular Biology, Arcispedale Santa Maria Nuova-IRCCS, Reggio Emilia, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Torricelli
authors.affiliation: Unit of Obstetrics and Gynaecology, Arcispedale Santa Maria Nuova-IRCCS, Reggio Emilia, Italy. dariomandato@virgilio.it.
authors.forename: Vincenzo Dario
authors.initials: VD
authors.lastname: Mandato
authors.affiliation: Laboratory of Molecular Biology, Arcispedale Santa Maria Nuova-IRCCS, Reggio Emilia, Italy.
authors.forename: Enrico
authors.initials: E
authors.lastname: Farnetti
authors.affiliation: Unit of Obstetrics and Gynaecology, Arcispedale Santa Maria Nuova-IRCCS, Reggio Emilia, Italy.
authors.forename: Martino
authors.initials: M
authors.lastname: Abrate
authors.affiliation: Laboratory of Molecular Biology, Arcispedale Santa Maria Nuova-IRCCS, Reggio Emilia, Italy.
authors.forename: Bruno
authors.initials: B
authors.lastname: Casali
authors.affiliation: Unit of Obstetrics and Gynaecology, Arcispedale Santa Maria Nuova-IRCCS, Reggio Emilia, Italy.
authors.forename: Gino
authors.initials: G
authors.lastname: Ciarlini
authors.affiliation: Unit of Obstetrics and Gynaecology, Arcispedale Santa Maria Nuova-IRCCS, Reggio Emilia, Italy.
authors.forename: Debora
authors.initials: D
authors.lastname: Pirillo
authors.affiliation: Unit of Pathology, Arcispedale Santa Maria Nuova-IRCCS, Reggio Emilia, Italy.
authors.forename: Maria Carolina
authors.initials: MC
authors.lastname: Gelli
authors.affiliation: Unit of Obstetrics and Gynaecology, Arcispedale Santa Maria Nuova-IRCCS, Reggio Emilia, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Costagliola
authors.affiliation: Laboratory of Molecular Biology, Arcispedale Santa Maria Nuova-IRCCS, Reggio Emilia, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Nicoli
authors.affiliation: Gynecologic Oncology Unit, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) - Centro di Riferimento Oncologico della Basilicata (CROB), Rionero in Vulture, Potenza, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Palomba
authors.affiliation: Unit of Obstetrics and Gynaecology, Arcispedale Santa Maria Nuova-IRCCS, Reggio Emilia, Italy.|Unit of Obstetrics and Gynaecology, University of Modena and Reggio Emilia, Reggio Emilia, Italy.
authors.forename: Giovanni Battista
authors.initials: GB
authors.lastname: La Sala
mesh_terms: D051546:Cyclooxygenase 2 / Q000235:genetics* / Q000378:metabolism; D004247:DNA / Q000032:analysis / Q000235:genetics; D016889:Endometrial Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D004717:Endometrium / Q000201:enzymology*; D005260:Female; D005500:Follow-Up Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007124:Immunoenzyme Techniques; D008875:Middle Aged; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D011110:Polymorphism, Genetic / Q000235:genetics*; D011379:Prognosis; D012189:Retrospective Studies
chemical_list: D004247:DNA; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human
keywords: COX 2; Endometrial cancer; Grading; PTGS2; rs5275