title: Model for probing membrane-cortex adhesion by micropipette aspiration and fluctuation spectroscopy.
abstract: We propose a model for membrane-cortex adhesion that couples membrane deformations, hydrodynamics, and kinetics of membrane-cortex ligands. In its simplest form, the model gives explicit predictions for the critical pressure for membrane detachment and for the value of adhesion energy. We show that these quantities exhibit a significant dependence on the active acto-myosin stresses. The model provides a simple framework to access quantitative information on cortical activity by means of micropipette experiments. We also extend the model to incorporate fluctuations and show that detailed information on the stability of membrane-cortex coupling can be obtained by a combination of micropipette aspiration and fluctuation spectroscopy measurements.
authors.affiliation: Departament d'Estructura i Constituents de la Matèria, Universitat de Barcelona, Barcelona, Spain.
authors.forename: Ricard
authors.initials: R
authors.lastname: Alert
authors.affiliation: Departament d'Estructura i Constituents de la Matèria, Universitat de Barcelona, Barcelona, Spain.
authors.forename: Jaume
authors.initials: J
authors.lastname: Casademunt
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Institute for Physics of Complex Systems, Dresden, Germany. Electronic address: brugues@mpi-cbg.de.
authors.forename: Jan
authors.initials: J
authors.lastname: Brugués
authors.affiliation: Laboratoire Gulliver, Centre National de la Recherche Scientifique-ESPCI Paris Tech, UMR 7083, Paris, France. Electronic address: pierre.sens@curie.fr.
authors.forename: Pierre
authors.initials: P
authors.lastname: Sens
mesh_terms: D008841:Actin Cytoskeleton / Q000737:chemistry / Q000378:metabolism; D002448:Cell Adhesion; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D006874:Hydrostatic Pressure; D008024:Ligands; D008954:Models, Biological; D013314:Stress, Mechanical; D014783:Viscosity
chemical_list: D008024:Ligands