title: [Studies on the interaction between malanin and metal ions by fluorescence spectra methods].
abstract: A novel protein with anti-tumor activities named malanin was isolated and purified from an endemic plant in Yunnan and Guangxi provinces. Effects of copper ion, silver ion and calcium ion on malanin and apo-malanin fluorescence spectra were studied. The results showed that copper ion leads to obvious statistic quenching of malanin and apo-malanin fluorescence. The dissociation constant of them from malanin and apo-malanin were about 2.37 X 10(-4) and 2.66 X 10(-4) mol · L(-1), respectively. The silver ion did not have quenching action on malanin fluorescence, but it had statistic quenching effect on apo-malanin fluorescence, and its dissociation constant was 2.37 X 10(-4) mol · L(-1). Calcium ion did not have quenching action on malanin and apo-malanin fluorescence. It plays an important role in keeping malanin natural conformation.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yuan
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Jian-hui
authors.initials: JH
authors.lastname: Dai
authors.forename: Xiao-fang
authors.initials: XF
authors.lastname: Liu
authors.forename: Hong-bin
authors.initials: HB
authors.lastname: Wang
authors.forename: Colin J
authors.initials: CJ
authors.lastname: Barrow
authors.forename: Wen-rong
authors.initials: WR
authors.lastname: Yang
mesh_terms: D002118:Calcium; D002681:China; D003300:Copper; D005453:Fluorescence; D006023:Glycoproteins / Q000737:chemistry*; D007477:Ions; D008670:Metals / Q000737:chemistry*; D012834:Silver; D013050:Spectrometry, Fluorescence; D014118:Toxins, Biological / Q000737:chemistry*
chemical_list: D006023:Glycoproteins; D007477:Ions; D008670:Metals; D014118:Toxins, Biological; C543356:malanin; D012834:Silver; D003300:Copper; D002118:Calcium