title: Cells on chip for multiplex screening.
abstract: Microarray technology was developed in the early 1990s to measure the transcription levels of thousands of genes in parallel. The basic premise of high-density arraying has since been expanded to create cells microarrays. Cells on chip are powerful experimental tools for high-throughput and multiplex screening of samples or cellular functions. Miniaturization increases assay throughput while reducing both reagent consumption and cell population heterogeneity effect, making these systems attractive for a wide range of assays, from drug discovery to toxicology, stem cell research and therapy. One form of cell microarrays, the transfected cell microarray, wherein plasmid DNA or siRNA, spotted on the surface of a substrate, is reverse-transfected locally into adherent cells, has become a standard tool for parallel cell-based analysis. With the advent of technologies, cells can also be directly spotted onto functionalized surfaces using robotic fluid-dispensing devices, or printed directly in bio-ink material. We are providing herein an overview of the last developments in optical cell microarrays allowing high-throughput and high-content analysis.
authors.affiliation: Institut de Chimie et Biochimie Moléculaires et Supramoléculaires Equipe Génie Enzymatique, Membranes Biomimétiques et Assemblages Supramoléculaires Université Lyon 1-CNRS 5246 ICBMS Bâtiment CPE 43, bd du 11 novembre 1918, 69622 Villeurbanne, Cedex, France.
authors.forename: Ophélie I
authors.initials: OI
authors.lastname: Berthuy
authors.affiliation: Institut de Chimie et Biochimie Moléculaires et Supramoléculaires Equipe Génie Enzymatique, Membranes Biomimétiques et Assemblages Supramoléculaires Université Lyon 1-CNRS 5246 ICBMS Bâtiment CPE 43, bd du 11 novembre 1918, 69622 Villeurbanne, Cedex, France.
authors.forename: Loïc J
authors.initials: LJ
authors.lastname: Blum
authors.affiliation: Institut de Chimie et Biochimie Moléculaires et Supramoléculaires Equipe Génie Enzymatique, Membranes Biomimétiques et Assemblages Supramoléculaires Université Lyon 1-CNRS 5246 ICBMS Bâtiment CPE 43, bd du 11 novembre 1918, 69622 Villeurbanne, Cedex, France. Electronic address: christophe.marquette@univ-lyon1.fr.
authors.forename: Christophe A
authors.initials: CA
authors.lastname: Marquette
mesh_terms: D015374:Biosensing Techniques; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D057166:High-Throughput Screening Assays; D006801:Humans; D008904:Miniaturization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010957:Plasmids / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics / Q000302:isolation & purification; D046888:Tissue Array Analysis / Q000379:methods*
chemical_list: D034741:RNA, Small Interfering; D004247:DNA
keywords: Bioprinting; Cell microarrays; High-throughput; Multiplex; Optical detection; Reverse transfection