title: Clonal dissemination of linezolid-resistant Staphylococcus capitis with G2603T mutation in domain V of the 23S rRNA and the cfr gene at a tertiary care hospital in China.
abstract: BACKGROUND
  The present study aims to investigate the potential mechanism of linezolid-resistant Staphylococcus capitis (LRSC) isolates collected from our hospital.
  
  
  METHODS
  The susceptibilities of 5 Staphylococcus capitis isolates displaying resistance towards linezolid were determined by E-test. Polymerase chain reactions (PCRs) and DNA sequencing were used to investigate the potential molecular mechanism. Clonal relatedness between these strains was analyzed by pulsed-field gel electrophoresis (PFGE).
  
  
  RESULTS
  The MICs of linezolid on these 5 isolates were >256 μg/mL. The G2603T mutation was observed in the domain V of the 23S rRNA with cfr gene being also widely detected among these 5 strains. PFGE analysis displayed close genetic relatedness between these linezolid-resistant isolates.
  
  
  CONCLUSIONS
  The emergence of LRSC isolates carrying G2603T mutation in the domain V of the 23S rRNA and harboring cfr gene in our hospital may pose a potential challenge to the public health.
authors.affiliation: Department of Laboratory Medicine, Nanjing Drum Tower Hospital, the affiliated Hospital of Nanjing University Medical School, 321# Zhongshan Road, Gulou District, Nanjing, Jiangsu Province, 210008, PR China. zwq_096@163.com.
authors.forename: Wanqing
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Department of Laboratory Medicine, Nanjing Jinling Hospital, the affiliated Hospital of Nanjing University Medical School, 305# East Zhongshan Road, Baixia District, Nanjing, Jiangsu Province, 210002, PR China. ndm082@163.com.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Niu
authors.affiliation: Department of Laboratory Medicine, Nanjing Drum Tower Hospital, the affiliated Hospital of Nanjing University Medical School, 321# Zhongshan Road, Gulou District, Nanjing, Jiangsu Province, 210008, PR China. cao-xiao-li@163.com.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Cao
authors.affiliation: Department of Laboratory Medicine, Nanjing Drum Tower Hospital, the affiliated Hospital of Nanjing University Medical School, 321# Zhongshan Road, Gulou District, Nanjing, Jiangsu Province, 210008, PR China. nmz780714@sina.com.
authors.forename: Mingzhe
authors.initials: M
authors.lastname: Ning
authors.affiliation: Department of Laboratory Medicine, Nanjing Drum Tower Hospital, the affiliated Hospital of Nanjing University Medical School, 321# Zhongshan Road, Gulou District, Nanjing, Jiangsu Province, 210008, PR China. njzhangzf@hotmail.com.
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Laboratory Medicine, Nanjing Drum Tower Hospital, the affiliated Hospital of Nanjing University Medical School, 321# Zhongshan Road, Gulou District, Nanjing, Jiangsu Province, 210008, PR China. shenhan10366@sina.com.
authors.forename: Han
authors.initials: H
authors.lastname: Shen
authors.affiliation: Department of Laboratory Medicine, Nanjing Drum Tower Hospital, the affiliated Hospital of Nanjing University Medical School, 321# Zhongshan Road, Gulou District, Nanjing, Jiangsu Province, 210008, PR China. zk60666@163.com.
authors.forename: Kui
authors.initials: K
authors.lastname: Zhang
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002681:China / Q000453:epidemiology; D003428:Cross Infection / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology; D024881:Drug Resistance, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D000069349:Linezolid / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D009154:Mutation; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology; D013210:Staphylococcus / Q000187:drug effects* / Q000235:genetics; D063128:Tertiary Health Care
chemical_list: D000900:Anti-Bacterial Agents; D012338:RNA, Ribosomal, 23S; D000069349:Linezolid