title: Lycium barbarum polysaccharide attenuates type II collagen-induced arthritis in mice.
abstract: No curative treatment is yet available for rheumatoid arthritis (RA), wherein chronic synovitis progresses to cartilage and bone destruction. Considering the recently recognized anti-inflammatory properties of Lycium barbarum polysaccharide (LBP; a derivative of the goji berry), we established the collagen type II-induced arthritis (CIA) mouse model to investigate the potential therapeutic effects and mechanisms of LBP. The CIA-induced changes and LBP-related effects were assessed by micro-computed tomography measurement of bone volume/tissue volume and by ELISA and western blotting detection of inflammatory mediators and matrix metalloproteinases (MMPs). The CIA mice showed substantial bone damage, bone loss, and increased concentrations of TNF-α, IL-6, IL-17, PGE2, MIP-1, anti-type II collagen IgG, MMP-1, and MMP-3. LBP treatments produced significant dose-dependent improvements in CIA-induced bone damage and bone loss, and significantly reduced CIA-stimulated expression of the inflammatory mediators and MMPs. Thus, LBP therapy can preserve bone integrity in CIA mice, possibly through down-regulation of inflammatory mediators.
authors.affiliation: Department of Pharmacy, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Pharmacy, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Lv
authors.affiliation: Department of Pharmacy, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.affiliation: Department of Pharmacy, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Immunology, Third Military Medical University, Chongqing 400038, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Tian
authors.affiliation: Department of Pharmacy, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Department of Immunology, Third Military Medical University, Chongqing 400038, China.
authors.forename: Di
authors.initials: D
authors.lastname: Yang
authors.affiliation: Department of Pharmacy, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ouyang
authors.affiliation: Department of Pharmacy, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
authors.forename: Fengjun
authors.initials: F
authors.lastname: Sun
authors.affiliation: Department of Pharmacy, Southwest Hospital, Third Military Medical University, Chongqing 400038, China. Electronic address: swhshiy@tom.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Department of Pharmacy, Southwest Hospital, Third Military Medical University, Chongqing 400038, China. Electronic address: peiyuan_xia@aliyun.com.
authors.forename: Peiyuan
authors.initials: P
authors.lastname: Xia
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000494:pharmacology*; D001169:Arthritis, Experimental / Q000175:diagnosis / Q000188:drug therapy* / Q000378:metabolism; D001842:Bone and Bones / Q000378:metabolism / Q000473:pathology; D054405:Chemokine CCL3 / Q000097:blood / Q000378:metabolism; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D018836:Inflammation Mediators / Q000097:blood / Q000378:metabolism; D007596:Joints / Q000473:pathology; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D019278:Matrix Metalloproteinase 3 / Q000378:metabolism; D051379:Mice; D055114:X-Ray Microtomography
chemical_list: D000893:Anti-Inflammatory Agents; D054405:Chemokine CCL3; D004365:Drugs, Chinese Herbal; D018836:Inflammation Mediators; C500223:lycium barbarum polysaccharide; D019278:Matrix Metalloproteinase 3; D020781:Matrix Metalloproteinase 1
keywords: Collagen-induced arthritis; Inflammatory mediators; Lycium barbarum polysaccharide