title: Multiple cyp51A-based mechanisms identified in azole-resistant isolates of Aspergillus fumigatus from China.
abstract: Seventy-two A. fumigatus clinical isolates from China were investigated for azole resistance based on mutations of cyp51A. We identified four azole-resistant strains, among which we found three strains highly resistant to itraconazole, two of which exhibit the TR34/L98H/S297T/F495I mutation, while one carries only the TR34/L98H mutation. To our knowledge, the latter has not been found previously in China. The fourth multiazole-resistant isolate (with only moderate itraconazole resistance) carries a new G432A mutation.
authors.affiliation: Department of Medical Mycology, Institute of Dermatology, Chinese Academy of Medical Science and Peking Union Medical College, Nanjing, Jiangsu, People's Republic of China.
authors.forename: Musang
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Medical Mycology, Institute of Dermatology, Chinese Academy of Medical Science and Peking Union Medical College, Nanjing, Jiangsu, People's Republic of China.
authors.forename: Rong
authors.initials: R
authors.lastname: Zeng
authors.affiliation: Department of Medical Mycology, Institute of Dermatology, Chinese Academy of Medical Science and Peking Union Medical College, Nanjing, Jiangsu, People's Republic of China.
authors.forename: Lili
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Medical Mycology, Institute of Dermatology, Chinese Academy of Medical Science and Peking Union Medical College, Nanjing, Jiangsu, People's Republic of China Georgetown University Medical Center, Department of Microbiology and Immunology, Washington, DC, USA.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Medical Mycology, Institute of Dermatology, Chinese Academy of Medical Science and Peking Union Medical College, Nanjing, Jiangsu, People's Republic of China.
authors.forename: Guixia
authors.initials: G
authors.lastname: Lv
authors.affiliation: Department of Medical Mycology, Institute of Dermatology, Chinese Academy of Medical Science and Peking Union Medical College, Nanjing, Jiangsu, People's Republic of China.
authors.forename: Yongnian
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Department of Medical Mycology, Institute of Dermatology, Chinese Academy of Medical Science and Peking Union Medical College, Nanjing, Jiangsu, People's Republic of China.
authors.forename: Hailin
authors.initials: H
authors.lastname: Zheng
authors.affiliation: Dermatology Department, Huashan Hospital, Fudan University, Shanghai, People's Republic of China.
authors.forename: Qiangqiang
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Dermatology Department, Tongji Hospital, Tongji University, Shanghai, People's Republic of China.
authors.forename: Jingjun
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Medical School, Nanjing University, Nanjing, Jiangsu, People's Republic of China.
authors.forename: Nan
authors.initials: N
authors.lastname: Zheng
authors.affiliation: Department of Medical Mycology, Institute of Dermatology, Chinese Academy of Medical Science and Peking Union Medical College, Nanjing, Jiangsu, People's Republic of China liumyco@hotmail.com.
authors.forename: Weida
authors.initials: W
authors.lastname: Liu
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D001228:Aspergillosis / Q000382:microbiology*; D001232:Aspergillus fumigatus / Q000187:drug effects* / Q000235:genetics; D001393:Azoles / Q000494:pharmacology*; D002681:China; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism*; D025141:Drug Resistance, Fungal / Q000187:drug effects*; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism*; D005838:Genotype; D006801:Humans; D017964:Itraconazole / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics
chemical_list: D000935:Antifungal Agents; D001393:Azoles; D005656:Fungal Proteins; D017964:Itraconazole; D003577:Cytochrome P-450 Enzyme System; C465526:cytochrome P-450 CYP51A, Aspergillus