title: Suppressive effect of pectic polysaccharides extracted from Rauwolfia verticillata (Lour.) Baill.var.hainanensis Tsiang on inflammation by regulation of NF- κ B pathway and interleukin-17 in mice with dextran sulphatesodium-induced ulcerative colitis.
abstract: OBJECTIVE
  To investigate the effects of pectic polysaccharides extracted from Rauwolfia verticillata (Lour.) Baill.var.hainanensis Tsiang on an experimental murine colitis model.
  
  
  METHODS
  Experimental colitis was induced by dextran sulfate sodium (DSS), and mice were divided into 4 groups: control, DSS alone, DSS plus SASP, DSS plus pectic polysaccharides. The disease activity index (DAI) and histological score were observed. The tumor necrosis factor (TNF)- α and interleukin (IL)-17 levels were measured by enzyme-linked immunosorbent assay. I κ B and NF- κ B p65 expression were assessed by western blot analysis. Myeloperoxidase (MPO) activity was determined by using MPO assay kit.
  
  
  RESULTS
  Administration of pectic polysaccharides significantly reduced the severity of DSS-induced colitis as assessed by DAI and histological score, and resulted in down regulation of MPO activity and NF- κ B p65 expression and subsequent degradation of I κ B protein, strikingly reduced the production of TNF- a and IL-17.
  
  
  CONCLUSIONS
  Pectic polysaccharides extracted from Rauvolfia verticillata (Lour.)Baill.var. hainanensis Tsiang exerts beneficial effects in experimental colitis and may therefore provide a useful therapeutic approach for the treatment of UC.
authors.affiliation: Department of Gastroenterology, Hainan Provincial People's Hospital, Haikou City 570311, Hainan Province, China. Electronic address: miaoxinpu@163.com.
authors.forename: Xin-Pu
authors.initials: XP
authors.lastname: Miao
authors.affiliation: Department of Gastroenterology, Hainan Provincial People's Hospital, Haikou City 570311, Hainan Province, China.
authors.forename: Xiao-Ning
authors.initials: XN
authors.lastname: Sun
authors.affiliation: Department of Gastroenterology, Hainan Provincial People's Hospital, Haikou City 570311, Hainan Province, China.
authors.forename: Lu-Jia
authors.initials: LJ
authors.lastname: Cui
authors.affiliation: Department of Gastroenterology, Hainan Provincial People's Hospital, Haikou City 570311, Hainan Province, China.
authors.forename: Qin-Fang
authors.initials: QF
authors.lastname: Cao
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Hainan Medical College, Haikou City 570103, Hainan Province, China.
authors.forename: Gui-Feng
authors.initials: GF
authors.lastname: Zhuang
authors.affiliation: Department of Gastroenterology, Hainan Provincial People's Hospital, Haikou City 570311, Hainan Province, China.
authors.forename: Tao-Zhi
authors.initials: TZ
authors.lastname: Deng
authors.affiliation: Department of Gastroenterology, Hainan Provincial People's Hospital, Haikou City 570311, Hainan Province, China.
authors.forename: Dong-Yan
authors.initials: DY
authors.lastname: Zhang
keywords: Dextran sulfate sodium–induced colitis; Interleukin–17; Nuclear factor; Pectic polysaccharides; Ulcerative colitis