title: First description and expression analysis of tumor necrosis factor receptor-associated factor 6 (TRAF6) from the swimming crab, Portunus trituberculatus.
abstract: Tumor necrosis factor receptor-associated factor 6 (TRAF6) is a cytoplasmic adapter protein that mediates signals induced by the tumor necrosis factor receptor (TNFR) superfamily and the interleukin-1 receptor (IL-1R). In the present study, the full-length cDNA of TRAF6 (Pt-TRAF6) was identified in a marine crab, Portunus trituberculatus. Pt-TRAF6 ORF is predicted to encode a 599-amino acid protein, including a RING type zinc finger, two TRAF-type zinc fingers, and a meprin and TRAF homology (MATH) domain. The overall amino acid sequence identity between Pt-TRAF6 and other TRAF6s ranged from 50.9 to 51.3% for shrimp and from 16.1 to 19.4% for insects. The Pt-TRAF6 gene contains six exons and five introns, which is different from the organization of the insect TRAF6 gene. Pt-TRAF6 transcripts were broadly expressed in all tissues tested, and their expression was higher in hemocytes, gills, the intestine, and heart than in muscle. Interestingly, the level of Pt-TRAF6 transcript differed between male and female crabs. After Vibrio alginolyticus or lipopolysaccharide (LPS) challenge, the Pt-TRAF6 transcript was down-regulated in hemocytes and up-regulated in gills. Moreover, Pt-TRAF6 expression was altered sooner in the LPS challenge group than in the V. alginolyticus challenge group. These results indicate that Pt-TRAF6 may respond to Gram-negative bacterial infections.
authors.affiliation: Key Laboratory of the Ministry of Education for Applied Marine Biotechnology, School of Marine Science, Ningbo University, Ningbo 315211, China.
authors.forename: Su-Ming
authors.initials: SM
authors.lastname: Zhou
authors.affiliation: Key Laboratory of the Ministry of Education for Applied Marine Biotechnology, School of Marine Science, Ningbo University, Ningbo 315211, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.affiliation: Key Laboratory of the Ministry of Education for Applied Marine Biotechnology, School of Marine Science, Ningbo University, Ningbo 315211, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Yang
authors.affiliation: Key Laboratory of the Ministry of Education for Applied Marine Biotechnology, School of Marine Science, Ningbo University, Ningbo 315211, China.
authors.forename: Shun
authors.initials: S
authors.lastname: Liu
authors.affiliation: Key Laboratory of the Ministry of Education for Applied Marine Biotechnology, School of Marine Science, Ningbo University, Ningbo 315211, China.
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Yuan
authors.affiliation: Key Laboratory of the Ministry of Education for Applied Marine Biotechnology, School of Marine Science, Ningbo University, Ningbo 315211, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Tao
authors.affiliation: Key Laboratory of the Ministry of Education for Applied Marine Biotechnology, School of Marine Science, Ningbo University, Ningbo 315211, China. Electronic address: wangguoliang@nbu.edu.cn.
authors.forename: Guo-Liang
authors.initials: GL
authors.lastname: Wang
mesh_terms: D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics* / Q000378:metabolism; D003386:Brachyura / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D005260:Female; D008070:Lipopolysaccharides / Q000008:administration & dosage; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D020539:Sequence Analysis, Protein; D012727:Sex Characteristics; D048029:TNF Receptor-Associated Factor 6 / Q000235:genetics* / Q000378:metabolism; D044149:Vibrio alginolyticus / Q000502:physiology
chemical_list: D060829:Arthropod Proteins; D018076:DNA, Complementary; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D048029:TNF Receptor-Associated Factor 6
keywords: Gene structure; LPS; Portunus trituberculatus; TRAF6; Vibrio alginolyticus