title: Characterization of Compounds in Psoralea corylifolia Using High-Performance Liquid Chromatography Diode Array Detection, Time-of-Flight Mass Spectrometry and Quadrupole Ion Trap Mass Spectrometry.
abstract: High-performance liquid chromatography with diode array detection (HPLC-DAD), time-of-flight mass spectrometry (HPLC-TOFMS) and quadrupole ion trap mass spectrometry (HPLC-QITMS) were used for separation and identification of multi-components in Psoralea corylifolia. Benefiting from combining the accurate mass measurement of HPLC-TOFMS to generate elemental compositions, the complementary multilevel structural information provided by HPLC-QITMS and the characteristic UV spectra obtained from HPLC-DAD, 24 components in P. corylifolia were identified. The five groups of isomers were differentiated based on the fragmentation behaviors in QITMS and UV spectra. It can be concluded that an effective method based on the combination of HPLC-DAD, HPLC-TOFMS and HPLC-QITMS for identification of chemical components in P. corylifolia was established. The results provide essential data for further pharmacological and clinical studies of P. corylifolia and facilitate the rapid quality control of the crude drug.
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, China Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
authors.forename: Guangguo
authors.initials: G
authors.lastname: Tan
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Tiehong
authors.initials: T
authors.lastname: Yang
authors.affiliation: Department of Business, The People's Liberation Army Troop 62370, Beijing 100071, China.
authors.forename: Huayan
authors.initials: H
authors.lastname: Miao
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Chai
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, China hongwuxa@hotmail.com hongwuxa@163.com.
authors.forename: Hong
authors.initials: H
authors.lastname: Wu
mesh_terms: D001572:Benzofurans / Q000032:analysis / Q000737:chemistry; D047188:Chalcones / Q000032:analysis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005363:Ficusin / Q000032:analysis / Q000737:chemistry; D005638:Fruit / Q000737:chemistry*; D006027:Glycosides / Q000032:analysis / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry*; D029907:Psoralea / Q000737:chemistry*
chemical_list: D001572:Benzofurans; D047188:Chalcones; D006027:Glycosides; D010936:Plant Extracts; C511397:psoralenoside; C468754:isobavachalcone; D005363:Ficusin