title: sCLU regulates cisplatin chemosensitivity of lung cancer cells in vivo.
abstract: BACKGROUND
  In a previous analysis using a lung cancer cell line model, we have found that therapies directed against secreted clusterin (sCLU) and its downstream signaling targets pAkt and pERK1/2 may have the potential to enhance the efficacy of cisplatin (DDP)-based chemotherapy in vitro. Here, we investigated the therapies directed against sCLU on the DDP-based chemotherapy in vivo and explored the mechanism.
  
  
  METHODS
  Using lung cancer cell lines, A549 cells and DDP-resistant A549 cells (A549(DDP)), we determined the effect of sCLU silencing using short interfering double-stranded RNA (siRNA) on chemosensitivity in immunocompromised mice bearing A549(DDP) tumors. We then determined the effect of sCLU overexpression via stable sCLU transfection on chemosensitivity in immunocompromised mice bearing A549 tumors. The effect of sCLU silencing or overexpression on pAkt and pERK1/2 expression and chemosensitivity in vivo was detected by Western blot assay.
  
  
  RESULTS
  The results showed sCLU silencing increased the chemosensitivity of A549(DDP) cells to DDP in vivo via downregulation of pAkt and pERK1/2 expression. And sCLU overexpression decreased the chemosensitivity of A549 cells to DDP in vivo via upregulation of pAkt and pERK1/2 expression.
  
  
  CONCLUSIONS
  We therefore concluded that the DDP-induced sCLU activation, which involved induction of pAkt and pERK1/2 activation that confer DDP resistance in immunocompromised mice and alteration of this balance, allows sensitization to the antitumor activity of cisplatin chemotherapy.
authors.affiliation: Department of Clinical Lab, People's Hospital of Laiwu, Laiwu, China. maguoliang7782@sina.com.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Ma
authors.affiliation: Department of neurology, People's Hospital of Zhangqiu, Zhangqiu, China. 152943265@qq.com.
authors.forename: Hengjuan
authors.initials: H
authors.lastname: Cai
authors.affiliation: Department of Clinical Lab, People's Hospital of Zhangqiu, Zhangqiu, China. chinazqglz@163.com.
authors.forename: Lizhen
authors.initials: L
authors.lastname: Gao
authors.affiliation: Department of Clinical Lab, People's Hospital of Zhangqiu, Zhangqiu, China. lungcancerw@126.com.
authors.forename: Mei
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Blood Transfusion, The Affiliated Hospital of Weifang Medical College, Weifang, China. whxblood@126.com.
authors.forename: Haixia
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D049109:Cell Proliferation / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology*; D051152:Clusterin / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D019008:Drug Resistance, Neoplasm; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D034741:RNA, Small Interfering / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; C495242:CLU protein, human; D051152:Clusterin; D034741:RNA, Small Interfering; D002945:Cisplatin