title: Vetiver oil (Java) attenuates cisplatin-induced oxidative stress, nephrotoxicity and myelosuppression in Swiss albino mice.
abstract: Clinical efficacy of the widely used anticancer drug cisplatin is limited due to its adverse side effects in normal tissues mediated by oxidative stress. This study was aimed to investigate the protective effects of vetiver acetate oil, Java (VO) against cisplatin-induced toxicity in Swiss albino mice. The ameliorating potential was evaluated by orally priming the animals with VO at doses 5, 10 or 20 mg/kg bw for 7 days prior to cisplatin treatment. Acute toxicity in mice was induced by injecting cisplatin (3 mg/kg bw) intraperitoneally for 5 consecutive days. Significant attenuation of renal toxicity was confirmed by histopathological examination, lowered levels of serum blood urea nitrogen, creatinine and reduced DNA damage. VO also compensated deficits in the renal antioxidant system. VO intervention significantly inhibited DNA damage, clastogenic effects, and cell cycle arrest in the bone marrow cells of mice. Hematological parameters indicated attenuation of cisplatin-induced myelosuppression. Overall, this study provides for the first time that VO has a protective role in the abatement of cisplatin-induced toxicity in mice which may be attributed to its antioxidant activity.
authors.affiliation: Cell Biology and Genetic Toxicology Laboratory, Department of Botany, Centre of Advanced Study, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700019, India.
authors.forename: Sonali
authors.initials: S
authors.lastname: Sinha
authors.affiliation: Cell Biology and Genetic Toxicology Laboratory, Department of Botany, Centre of Advanced Study, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700019, India.
authors.forename: Manivannan
authors.initials: M
authors.lastname: Jothiramajayam
authors.affiliation: Cell Biology and Genetic Toxicology Laboratory, Department of Botany, Centre of Advanced Study, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700019, India.
authors.forename: Manosij
authors.initials: M
authors.lastname: Ghosh
authors.affiliation: Cell Biology and Genetic Toxicology Laboratory, Department of Botany, Centre of Advanced Study, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700019, India.
authors.forename: Aditi
authors.initials: A
authors.lastname: Jana
authors.affiliation: Department of Zoology, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700019, India.
authors.forename: Urmi
authors.initials: U
authors.lastname: Chatterji
authors.affiliation: Cell Biology and Genetic Toxicology Laboratory, Department of Botany, Centre of Advanced Study, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700019, India. Electronic address: anitamukherjee28@gmail.com.
authors.forename: Anita
authors.initials: A
authors.lastname: Mukherjee
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000009:adverse effects; D000975:Antioxidants / Q000494:pharmacology; D001806:Blood Urea Nitrogen; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000378:metabolism; D002945:Cisplatin / Q000008:administration & dosage / Q000009:adverse effects*; D003404:Creatinine / Q000097:blood; D004249:DNA Damage / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000097:blood; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D018384:Oxidative Stress / Q000187:drug effects*; D010938:Plant Oils / Q000494:pharmacology*; D023382:Toxicity Tests, Acute
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D010938:Plant Oils; D003404:Creatinine; D005978:Glutathione; D002945:Cisplatin
keywords: Cisplatin; Genotoxicity; Myelosuppression; Nephrotoxicity; Oxidative stress; Vetiver acetate oil