title: Identification of the peptide derived from S1 domain that inhibits type I and type II feline infectious peritonitis virus infection.
abstract: Feline infectious peritonitis virus (FIPV) can cause a lethal disease in cats, feline infectious peritonitis (FIP). A therapeutic drug that is effective against FIP has not yet been developed. Peptides based on viral protein amino acid sequences have recently been attracting attention as new antiviral drugs. In the present study, we synthesized 30 overlapping peptides based on the amino acid sequence of the S1 domain of the type I FIPV strain KU-2 S protein, and investigated their inhibitory effects on FIPV infection. To evaluate the inhibitory effects on type I FIPV infection of these peptides, we investigated a method to increase the infection efficiency of poorly replicative type I FIPV. The efficiency of type I FIPV infection was increased by diluting the virus with medium containing a polycation. Of the 30 peptides, I-S1-8 (S461-S480), I-S1-9 (S471-S490), I-S1-10 (S481-S500), I-S1-16 (S541-S560), and I-S1-22 (S601-S620) significantly decreased the infectivity of FIPV strain KU-2 while I-S1-9 and I-S1-16 exhibited marked inhibitory effects on FIPV infection. The inhibitory effects on FIPV infection of these 2 peptides on other type I and type II FIPV strains, feline herpesvirus (FHV), and feline calicivirus (FCV) were also examined. These 2 peptides specifically inhibited type I and type II FIPV, but did FHV or FCV infection. In conclusion, the possibility of peptides derived from the S protein of type I FIPV strain KU-2 as anti-FIPV agents effective not only for type I, but also type II FIPV was demonstrated in vitro.
authors.affiliation: Laboratory of Veterinary Infectious Disease, School of Veterinary Medicine, Kitasato University, Towada, Aomori 034-8628, Japan. Electronic address: dv12003f@st.kitasato-u.ac.jp.
authors.forename: Tomoyoshi
authors.initials: T
authors.lastname: Doki
authors.affiliation: Laboratory of Veterinary Infectious Disease, School of Veterinary Medicine, Kitasato University, Towada, Aomori 034-8628, Japan. Electronic address: takanot@vmas.kitasato-u.ac.jp.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Takano
authors.affiliation: Laboratory of Veterinary Infectious Disease, School of Veterinary Medicine, Kitasato University, Towada, Aomori 034-8628, Japan. Electronic address: tachikoma893@yahoo.co.jp.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Koyama
authors.affiliation: Laboratory of Veterinary Infectious Disease, School of Veterinary Medicine, Kitasato University, Towada, Aomori 034-8628, Japan. Electronic address: hohdatsu@vmas.kitasato-u.ac.jp.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Hohdatsu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology*; D000998:Antiviral Agents; D002415:Cats; D016765:Coronavirus, Feline / Q000187:drug effects* / Q000276:immunology; D016766:Feline Infectious Peritonitis / Q000188:drug therapy*; D010455:Peptides / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D013047:Specific Pathogen-Free Organisms; D064370:Spike Glycoprotein, Coronavirus / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000998:Antiviral Agents; D010455:Peptides; D064370:Spike Glycoprotein, Coronavirus; C578556:spike protein, feline infectious peritonitis virus
keywords: Feline coronavirus; Feline infectious peritonitis; Peptide