title: Antibacterial properties of cetyltrimethylammonium bromide-stabilized green silver nanoparticles against methicillin-resistant Staphylococcus aureus.
abstract: Silver nanoparticles (AgNPs) were synthesized using Artemisia capillaris extracts in the absence and presence of stabilizers, and the antibacterial activities of the AgNPs against methicillin-resistant Staphylococcus aureus (MRSA) were determined by a minimum inhibitory concentration (MIC) assay. Two stabilizers, cetyltrimethylammonium bromide (CTAB) and sodium dodecyl sulfate (SDS), were utilized during the one-step synthesis to increase the colloidal stability of the AgNPs. The extract of A. capillaris was used as a reducing agent to convert silver ions into AgNPs. Sharp surface plasmon resonance bands in the range of 423-426 nm were observed in the UV-Visible spectra of the AgNPs, which indicated that the AgNPs were predominantly spherical shaped. Their average diameter, which ranged from 15.11 to 16.54 nm, was measured from high-resolution transmission electron microscopy images. Remarkably, the CTAB-stabilized AgNPs exhibited greater antibacterial activity (2- to 8-fold increase, MIC 15.6-62.5 μg/mL) against MRSA compared with the extract, AgNPs prepared in the absence of stabilizers, and SDS-stabilized AgNPs. The results indicate that CTAB-stabilized AgNPs prepared using plant extracts as reducing agents are very promising for the development of novel antibacterial agents.
authors.affiliation: College of Pharmacy, Inje University, 197 Inje-ro, Gimhae, Gyeongnam, 621-749, Republic of Korea.
authors.forename: Hyoyeong
authors.initials: H
authors.lastname: Jang
authors.affiliation: College of Pharmacy, Inje University, 197 Inje-ro, Gimhae, Gyeongnam, 621-749, Republic of Korea.
authors.forename: Soo Hyeon
authors.initials: SH
authors.lastname: Lim
authors.affiliation: Department of Food and Life Science, Pukyong National University, Busan, 608-737, Republic of Korea.
authors.forename: Jae Sue
authors.initials: JS
authors.lastname: Choi
authors.affiliation: College of Pharmacy, Inje University, 197 Inje-ro, Gimhae, Gyeongnam, 621-749, Republic of Korea. youmiep@inje.ac.kr.
authors.forename: Youmie
authors.initials: Y
authors.lastname: Park
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology*; D019306:Artemisia / Q000737:chemistry*; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D005079:Excipients / Q000737:chemistry; D053768:Metal Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D046529:Microscopy, Electron, Transmission; D010316:Particle Size; D010936:Plant Extracts / Q000737:chemistry; D012834:Silver / Q000737:chemistry; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D002593:Cetrimonium Compounds; D005079:Excipients; D010936:Plant Extracts; D012967:Sodium Dodecyl Sulfate; D012834:Silver; D000077286:Cetrimonium
keywords: Artemisia capillaris; Cetyltrimethylammonium bromide; Methicillin-resistant Staphylococcus aureus; Silver nanoparticles