title: The chemical profile of pyrrolizidine alkaloids from selected greek endemic boraginaceae plants determined by gas chromatography/mass spectrometry.
abstract: Four Greek endemic Boraginaceae plants, Onosma erecta Sibth. & Sm., Onosma kaheirei Teppner, Onosma leptantha Heldr., and Cynoglossum columnae L. (aerial parts), were screened for their content of pyrrolizidine alkaloids (PAs). TLC with the Mattocks-Molyneux visualization reagent was used as a preliminary qualitative test for PA or PA N-oxide detection. The extracts of the species found to contain PAs and their N-oxides were further analyzed by GC/MS, so as to identify their structures by means of the mass spectra and retention index values of known PAs already published in the literature. Twenty-three PAs were identified. For additional peaks, recognized as possible PAs by their MS pattern, no exact structures were tentatively suggested, as a result of lack of matching literature data. Furthermore, a quantitative PA profile of the species was obtained.
authors.affiliation: University of Athens, School of Pharmacy, Department of Pharmacognosy, University Campus of Zografou, 157 71 Zografou, Athens, Greece.
authors.forename: Harilaos
authors.initials: H
authors.lastname: Damianakos
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Jeziorek
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Pietrosiuk
authors.forename: Ioanna
authors.initials: I
authors.lastname: Chinou
mesh_terms: D028082:Boraginaceae / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006115:Greece; D010936:Plant Extracts / Q000032:analysis; D011763:Pyrrolizidine Alkaloids / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D010936:Plant Extracts; D011763:Pyrrolizidine Alkaloids