title: Mangrovibacter yixingensis sp. nov., isolated from farmland soil.
abstract: A Gram-staining-negative, facultatively anaerobic, rod-shaped, nitrogen-fixing bacterial strain, designated TULL-AT, was isolated from a farmland soil sample in Yixing, China. The optimal conditions for growth were 30 °C, pH 7.0-8.0 and 0% (w/v) NaCl. Q8 was the dominant respiratory quinone and the major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and aminophospholipid. Phylogenetic analysis of 16S rRNA gene sequences showed that strain TULL-AT was most closely related to Mangrovibacter plantisponsor MSSRF40T (99.6%), followed by Salmonella enterica subsp. diarizonae DSM 14847T (96.8%) and Cronobacter condimenti 1330T (96.8 %). Sequence analysis of the genes rpoB, gyrB and hsp60 revealed that those of strain TULL-AT also exhibit high sequence similarity with those of the species M. plantisponsor MSSRF40T (95.5, 94.1 and 93.4%). The genomic DNA G+C content was 52 mol%. The major fatty acids of strain TULL-AT were C16 : 0, C16 : 1ω7c and/or C16 : 1ω6c, C18 : 1ω7c /C18 : 1ω6c, C14 : 0, C14 : 0 3-OH/iso-C16 : 1 I and iso-C17 : 1 I and/or anteiso-C17 : 1 B. Strain TULL-AT showed low DNA-DNA relatedness with M. plantisponsor MSSRF40T (35.10 ± 1.41%). Based on the multiple genotypic and phenotypic data, strain TULL-AT is considered to represent a novel species of the genus Mangrovibacter, for which the name Mangrovibacter yixingensis sp. nov. is proposed. The type strain is TULL-AT ( = ACCC 19709T = KCTC 42181T).
authors.affiliation: Key Laboratory of Microbiology of Agricultural Environment, Ministry of Agriculture, College of life sciences, Nanjing Agricultural University, Nanjing, 210095, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Microbiology of Agricultural Environment, Ministry of Agriculture, College of life sciences, Nanjing Agricultural University, Nanjing, 210095, PR China.
authors.forename: Su-Hui
authors.initials: SH
authors.lastname: Guo
authors.affiliation: Key Laboratory of Microbiology of Agricultural Environment, Ministry of Agriculture, College of life sciences, Nanjing Agricultural University, Nanjing, 210095, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Sun
authors.affiliation: Key Laboratory of Microbiology of Agricultural Environment, Ministry of Agriculture, College of life sciences, Nanjing Agricultural University, Nanjing, 210095, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Microbiology of Agricultural Environment, Ministry of Agriculture, College of life sciences, Nanjing Agricultural University, Nanjing, 210095, PR China.
authors.forename: Ming-Gen
authors.initials: MG
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Microbiology of Agricultural Environment, Ministry of Agriculture, College of life sciences, Nanjing Agricultural University, Nanjing, 210095, PR China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: Key Laboratory of Microbiology of Agricultural Environment, Ministry of Agriculture, College of life sciences, Nanjing Agricultural University, Nanjing, 210095, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Hong
authors.affiliation: Key Laboratory of Microbiology of Agricultural Environment, Ministry of Agriculture, College of life sciences, Nanjing Agricultural University, Nanjing, 210095, PR China.
authors.forename: Xing
authors.initials: X
authors.lastname: Huang
mesh_terms: D000383:Agriculture; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D004755:Enterobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005227:Fatty Acids / Q000737:chemistry; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030778:ubiquinone 8