title: [Effect of CKJ recipe containing serum on activation of rat primary hepatic stellate cells, TGF-beta1 and its receptors].
abstract: OBJECTIVE
  To observe the effect of CKJ Recipe (consisting of Cordyceps sinensis polysaccharide, amygdaloside, and gypenosides) containing serum on the activation of rat primary hepatic stellate cells (rHSCs) and to explore its pharmacological mechanism.
  
  
  METHODS
  rHSCs were isolated form liver and cultured for four days. Then they were divided into the normal control group, the model group, and the CKJ group. rHSCs in the model group and the CKJ group were treated with 2.5 ng/mL transforming growth factor beta1 (TGF-beta1) in serum-free DMEM for 24 h. Serum free DMEM (containing no TGF-beta1) was taken as the control for the normal control group. rHSCs in the CKJ group were treated with 5% CKJ-containing serum for 24 h. rHSCs in the other two groups were treated with 5% blank serum for 24 h.The protein expression level of a smooth muscle actin (alpha-SMA) was determined using high throughput screening (HCS) and Western blot. mRNA expression levels of alpha-SMA, collagen I (Col-I), platelet-derived growth factor receptor beta (PDGF-betaR), TGF-beta1, transforming growth factor beta receptor 1 (TGF-betaR1), and transforming growth factor beta receptor 2 (TGF-beta R2) were detected using quantitative RT-PCR.
  
  
  RESULTS
  Compared with the normal control group, the protein expression level of alpha-SMA, mRNA expression levels of alpha-SMA, Col-I, PDGF-betaR, TGF-beta1, TGF-betaR1, and TGF-betaR2 significantly increased in the model group (P<0.05, P<0.01). Compared with the model group, the protein expression level of alpha-SMA, mRNA expression levels of alpha-SMA, Col-I, PDGF-betaR, TGF-beta1, TGF-beta1, and TGF-beta R2 significantly decreased in the CKJ group (P<0.05, P<0.01).
  
  
  CONCLUSIONS
  CKJ containing serum could inhibit the protein expression level of o-SMA, which was probably related with inhibiting TGF-beta1 and its related receptors.
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.forename: Qin
authors.initials: Q
authors.lastname: Feng
authors.forename: Jing-hua
authors.initials: JH
authors.lastname: Peng
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.forename: Chun-geng
authors.initials: CG
authors.lastname: Liang
authors.forename: Ya-mei
authors.initials: YM
authors.lastname: Hai
authors.forename: Yi-yang
authors.initials: YY
authors.lastname: Hu
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D055166:Hepatic Stellate Cells / Q000378:metabolism*; D051381:Rats; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
chemical_list: D004365:Drugs, Chinese Herbal; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1