title: Development of a viability standard curve for microencapsulated probiotic bacteria using confocal microscopy and image analysis software.
abstract: Microencapsulation is proposed to protect probiotic strains from food processing procedures and to maintain probiotic viability. Little research has described the in situ viability of microencapsulated probiotics. This study successfully developed a real-time viability standard curve for microencapsulated bacteria using confocal microscopy, fluorescent dyes and image analysis software.
authors.affiliation: Science Department, School of Health & Science, University of Western Sydney, Locked Bag 1797, Penrith, NSW 2751, Australia.
authors.forename: Sarah
authors.initials: S
authors.lastname: Moore
authors.affiliation: Science Department, School of Health & Science, University of Western Sydney, Locked Bag 1797, Penrith, NSW 2751, Australia. Electronic address: k.kailasapathy@uws.edu.au.
authors.forename: Kasipathy
authors.initials: K
authors.lastname: Kailasapathy
authors.affiliation: Science Department, School of Health & Science, University of Western Sydney, Locked Bag 1797, Penrith, NSW 2751, Australia. Electronic address: m.phillips@uws.edu.au.
authors.forename: Michael
authors.initials: M
authors.lastname: Phillips
authors.affiliation: Science Department, School of Health & Science, University of Western Sydney, Locked Bag 1797, Penrith, NSW 2751, Australia. Electronic address: m.r.jones@uws.edu.au.
authors.forename: Mark R
authors.initials: MR
authors.lastname: Jones
mesh_terms: D007091:Image Processing, Computer-Assisted / Q000379:methods*; D050296:Microbial Viability; D018613:Microscopy, Confocal / Q000379:methods*; D019936:Probiotics; D012984:Software; D013678:Technology, Pharmaceutical / Q000379:methods*
keywords: Confocal microscopy; Image analysis software; Microencapsulation; Probiotic bacteria; Viability standard curve