title: Metabolite localization in living drosophila using High Resolution Magic Angle Spinning NMR.
abstract: We have developed new methods enabling in vivo localization and identification of metabolites through their (1)H NMR signatures, in a drosophila. Metabolic profiles in localized regions were obtained using HR-MAS Slice Localized Spectroscopy and Chemical Shift Imaging at high magnetic fields. These methods enabled measurement of metabolite contents in anatomic regions of the fly, demonstrated by a decrease in β-alanine signals in the thorax of flies showing muscle degeneration.
authors.affiliation: CNRS, CEMHTI UPR3079, Univ. Orléans, F-45071 Orléans, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Sarou-Kanian
authors.affiliation: CNRS, CBM UPR4301, Univ. Orléans, F-45071 Orléans, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Joudiou
authors.affiliation: CNRS, CBM UPR4301, Univ. Orléans, F-45071 Orléans, France.
authors.forename: Fanny
authors.initials: F
authors.lastname: Louat
authors.affiliation: CNRS, CEMHTI UPR3079, Univ. Orléans, F-45071 Orléans, France.
authors.forename: Maxime
authors.initials: M
authors.lastname: Yon
authors.affiliation: CNRS, CBM UPR4301, Univ. Orléans, F-45071 Orléans, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Szeremeta
authors.affiliation: CNRS, CBM UPR4301, Univ. Orléans, F-45071 Orléans, France.
authors.forename: Sandra
authors.initials: S
authors.lastname: Même
authors.affiliation: CNRS, CEMHTI UPR3079, Univ. Orléans, F-45071 Orléans, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Massiot
authors.affiliation: CNRS, CBM UPR4301, Univ. Orléans, F-45071 Orléans, France.
authors.forename: Martine
authors.initials: M
authors.lastname: Decoville
authors.affiliation: CNRS, CEMHTI UPR3079, Univ. Orléans, F-45071 Orléans, France.
authors.forename: Franck
authors.initials: F
authors.lastname: Fayon
authors.affiliation: CNRS, CBM UPR4301, Univ. Orléans, F-45071 Orléans, France.
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Beloeil
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000378:metabolism; D004330:Drosophila / Q000378:metabolism*; D005260:Female; D008297:Male; D055442:Metabolome; D066244:Proton Magnetic Resonance Spectroscopy; D013909:Thorax / Q000378:metabolism