title: in vitro development of bioimplants made up of elastomeric scaffolds with peptide gel filling seeded with human subcutaneous adipose tissue-derived progenitor cells.
abstract: Myocardial tissue lacks the ability to regenerate itself significantly following a myocardial infarction. Thus, new strategies that could compensate this lack are of high interest. Cardiac tissue engineering (CTE) strategies are a relatively new approach that aims to compensate the tissue loss using combination of biomaterials, cells and bioactive molecules. The goal of the present study was to evaluate cell survival and growth, seeding capacity and cellular phenotype maintenance of subcutaneous adipose tissue-derived progenitor cells in a new synthetic biomaterial scaffold platform. Specifically, here we tested the effect of the RAD16-I peptide gel in microporous poly(ethyl acrylate) polymers using two-dimensional PEA films as controls. Results showed optimal cell adhesion efficiency and growth in the polymers coated with the self-assembling peptide RAD16-I. Importantly, subATDPCs seeded into microporous PEA scaffolds coated with RAD16-I maintained its phenotype and were able to migrate outwards the bioactive patch, hopefully toward the infarcted area once implanted. These data suggest that this bioimplant (scaffold/RAD16-I/cells) can be suitable for further in vivo implantation with the aim to improve the function of affected tissue after myocardial infarction.
authors.affiliation: Tissue Engineering Laboratory, Bioengineering Department, Institut Químic De Sarrià, Universitat Ramon Llull, Barcelona, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Castells-Sala
authors.affiliation: Center for Biomaterials and Tissue Engineering, Universitat Politècnica De Valencia, Cno. De Vera S/N, Valencia, 46022, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Martínez-Ramos
authors.affiliation: Center for Biomaterials and Tissue Engineering, Universitat Politècnica De Valencia, Cno. De Vera S/N, Valencia, 46022, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Vallés-Lluch
authors.affiliation: Center for Biomaterials and Tissue Engineering, Universitat Politècnica De Valencia, Cno. De Vera S/N, Valencia, 46022, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Monleón Pradas
authors.affiliation: Tissue Engineering Laboratory, Bioengineering Department, Institut Químic De Sarrià, Universitat Ramon Llull, Barcelona, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Semino
mesh_terms: D000180:Acrylic Resins / Q000494:pharmacology; D001672:Biocompatible Materials / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D020724:Elastomers; D005782:Gels / Q000494:pharmacology*; D020869:Gene Expression Profiling; D006801:Humans; D018613:Microscopy, Confocal; D010455:Peptides / Q000494:pharmacology*; D011108:Polymers / Q000494:pharmacology*; D019736:Prostheses and Implants; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D050151:Subcutaneous Fat / Q000166:cytology*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000180:Acrylic Resins; D001672:Biocompatible Materials; D020724:Elastomers; D005782:Gels; D010455:Peptides; D011108:Polymers; C036925:elastomeric polymer; C478961:poly(ethylacrylate)
keywords: bioactive patch; elastomeric membrane; mesenchymal stem cells-like cells; self-assembling peptide