title: The surfactant dipalmitoylphophatidylcholine modifies acute responses in alveolar carcinoma cells in response to low-dose silver nanoparticle exposure.
abstract: Nanotechnology is a rapidly growing field with silver nanoparticles (AgNP) in particular utilized in a wide variety of consumer products. This has presented a number of concerns relating to exposure and the associated toxicity to humans and the environment. As inhalation is the most common exposure route, this study investigates the potential toxicity of AgNP to A549 alveolar epithelial carcinoma cells and the influence of a major component of lung surfactant dipalmitoylphosphatidylcholine (DPPC) on toxicity. It was illustrated that exposure to AgNP generated low levels of oxidative stress and a reduction in cell viability. While DPPC produced no significant effect on viability studies its presence resulted in increased reactive oxygen species formation. DPPC also significantly modified the inflammatory response generated by AgNP exposure. These findings suggest a possible interaction between AgNP and DPPC causing particles to become more reactive, thus increasing oxidative insult and inflammatory response within A549 cells.
authors.affiliation: Nanolab Research Centre, Focas Institute, Dublin Institute of Technology, Kevin Street, Dublin, 8, Ireland.
authors.forename: A
authors.initials: A
authors.lastname: Murphy
authors.affiliation: Nanolab Research Centre, Focas Institute, Dublin Institute of Technology, Kevin Street, Dublin, 8, Ireland.
authors.forename: K
authors.initials: K
authors.lastname: Sheehy
authors.affiliation: Nanolab Research Centre, Focas Institute, Dublin Institute of Technology, Kevin Street, Dublin, 8, Ireland.
authors.forename: A
authors.initials: A
authors.lastname: Casey
authors.affiliation: Nanolab Research Centre, Focas Institute, Dublin Institute of Technology, Kevin Street, Dublin, 8, Ireland.|School of Physics, Dublin Institute of Technology, Kevin Street, Dublin, 8, Ireland.
authors.forename: G
authors.initials: G
authors.lastname: Chambers
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000494:pharmacology*; D002282:Adenocarcinoma, Bronchiolo-Alveolar / Q000473:pathology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000473:pathology; D008175:Lung Neoplasms / Q000473:pathology*; D053768:Metal Nanoparticles / Q000633:toxicity*; D010078:Oxazines; D017382:Reactive Oxygen Species / Q000378:metabolism; D012834:Silver / Q000633:toxicity*; D013778:Tetrazolium Salts; D013844:Thiazoles; D014410:Tumor Stem Cell Assay; D014966:Xanthenes
chemical_list: D004396:Coloring Agents; D010078:Oxazines; D017382:Reactive Oxygen Species; D013778:Tetrazolium Salts; D013844:Thiazoles; D014966:Xanthenes; C005843:resazurin; D015060:1,2-Dipalmitoylphosphatidylcholine; D012834:Silver; C022616:thiazolyl blue
keywords: DPPC; acute response; inflammatory response; modify; reactive oxygen species; silver nanoparticles