title: Cultures of Schwann-like cells differentiated from adipose-derived stem cells on PDMS/MWNT sheets as a scaffold for peripheral nerve regeneration.
abstract: Carbon nanotubes (CNTs) are promising candidates as novel scaffolds for peripheral nerve regeneration. Schwann cells (SCs) are attractive therapeutic targets due to their pivotal role in peripheral nerve regeneration, but primary SCs have limitations for clinical application. However, adipose-derived stem cells (ASCs) may differentiate into Schwann-like cells. The present study assesses the potential applicability of multiwall CNTs (MWNTs) composited with polydimethylsiloxane (PDMS), which were then seeded with differentiated adipose-derived stem cells (dASCs) to promote neuronal differentiation and growth. Aqueous MWNT dispersion was filtered, and the PDMS/MWNT sheets were prepared using a simple printing-transfer method. Characterization of PDMS/MWNT sheets indicated their unique physical properties, such as superior mechanical strength and electroconductivity, compared with bare PDMS sheets. ASCs were differentiated into Schwann-like cells using a mixture of glial growth factors. Dorsal root ganglion (DRG) neurons were co-cultured with SCs and dASCs on PDMS/MWNTs sheets or noncoated dishes. An alamar blue proliferation assay of dASC and SCs showed significantly more dASC and SCs cultured on PDMS/MWNT sheets at 48 h and 72 h than when cultured on noncoated dishes (p < 0.05). Additionally, when DRG were cultured on PDMS/MWNT sheets seeded with dASCs, the proliferation of DRG neurons and the longest neurite outgrowth length per neuron were significantly greater than when DRG were cultured on PDMS/MWNT sheets alone or on noncoated dishes seeded with SCs or dASCs (p < 0.05). Overall, PDMS/MWNT sheets exhibited excellent biocompatibility for culturing Schwann-like cells differentiated from ASCs. Seeding the dASCs on PDMS/MWNT sheets may produce synergistic effects in peripheral nerve regeneration, similarly to SCs.
authors.affiliation: Department of Neurosurgery, Medical Research Institute, Pusan National University Hospital and School of Medicine, Busan, 602-739, Republic of Korea.
authors.forename: In Ho
authors.initials: IH
authors.lastname: Han
authors.affiliation: Department of Nano Fusion, and Cogno-Mechatronics Engineering, Pusan National University, Busan, 609-735, Republic of Korea.|Institute of Biomedical Engineering and Instruments, College of Life Information Science and Instrument Engineering, Hangzhou Dianzi University, Hangzhou, 310018, China.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Sun
authors.affiliation: Department of Neurosurgery, Medical Research Institute, Pusan National University Hospital and School of Medicine, Busan, 602-739, Republic of Korea.
authors.forename: Yoon Ji
authors.initials: YJ
authors.lastname: Choi
authors.affiliation: Department of Nano Fusion, and Cogno-Mechatronics Engineering, Pusan National University, Busan, 609-735, Republic of Korea.
authors.forename: Fengming
authors.initials: F
authors.lastname: Zou
authors.affiliation: Department of Neurosurgery, Medical Research Institute, Pusan National University Hospital and School of Medicine, Busan, 602-739, Republic of Korea.
authors.forename: Kyoung Hyup
authors.initials: KH
authors.lastname: Nam
authors.affiliation: Department of Neurosurgery, Medical Research Institute, Pusan National University Hospital and School of Medicine, Busan, 602-739, Republic of Korea.
authors.forename: Won Ho
authors.initials: WH
authors.lastname: Cho
authors.affiliation: Department of Neurosurgery, Medical Research Institute, Pusan National University Hospital and School of Medicine, Busan, 602-739, Republic of Korea.
authors.forename: Byung Kwan
authors.initials: BK
authors.lastname: Choi
authors.affiliation: Department of Neurosurgery, Pusan National University Yangsan Hospital, Yangsan, 626-770, Republic of Korea.
authors.forename: Geun Sung
authors.initials: GS
authors.lastname: Song
authors.affiliation: Department of Nano Fusion, and Cogno-Mechatronics Engineering, Pusan National University, Busan, 609-735, Republic of Korea.
authors.forename: Kwangnak
authors.initials: K
authors.lastname: Koh
authors.affiliation: Department of Nano Fusion, and Cogno-Mechatronics Engineering, Pusan National University, Busan, 609-735, Republic of Korea.
authors.forename: Jaebeom
authors.initials: J
authors.lastname: Lee
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004129:Dimethylpolysiloxanes / Q000494:pharmacology*; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D037742:Nanotubes, Carbon / Q000737:chemistry; D009416:Nerve Regeneration / Q000187:drug effects*; D016501:Neurites / Q000187:drug effects / Q000378:metabolism; D010525:Peripheral Nerves / Q000502:physiology*; D017207:Rats, Sprague-Dawley; D009418:S100 Proteins / Q000378:metabolism; D012583:Schwann Cells / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D013050:Spectrometry, Fluorescence; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D004129:Dimethylpolysiloxanes; D037742:Nanotubes, Carbon; D009418:S100 Proteins; C013830:baysilon
keywords: Schwann cell; adipose-derived stem cells; carbon nanotube; polydimethylsiloxane; primary neuronal cell growth control