title: Heterologous surface display on lactic acid bacteria: non-GMO alternative?
abstract: Lactic acid bacteria (LAB) are food-grade hosts for surface display with potential applications in food and therapy. Alternative approaches to surface display on LAB would avoid the use of recombinant DNA technology and genetically-modified organism (GMO)-related regulatory requirements. Non-covalent surface display of proteins can be achieved by fusing them to various cell-wall binding domains, of which the Lysine motif domain (LysM) is particularly well studied. Fusion proteins have been isolated from recombinant bacteria or from their growth medium and displayed on unmodified bacteria, enabling heterologous surface display. This was demonstrated on non-viable cells devoid of protein content, termed bacteria-like particles, and on various species of genus Lactobacillus. Of the latter, Lactobacillus salivarius ATCC 11741 was recently shown to be particularly amenable for LysM-mediated display. Possible regulatory implications of heterologous surface display are discussed, particularly those relevant for the European Union.
authors.affiliation: a Department of Biotechnology ; Jožef Stefan Institute ; Ljubljana , Slovenia.
authors.forename: Petra
authors.initials: P
authors.lastname: Zadravec
authors.forename: Borut
authors.initials: B
authors.lastname: Štrukelj
authors.forename: Aleš
authors.initials: A
authors.lastname: Berlec
mesh_terms: D061505:Cell Surface Display Techniques / Q000379:methods*; D002473:Cell Wall / Q000737:chemistry*; D007778:Lactobacillus / Q000737:chemistry* / Q000145:classification; D030781:Organisms, Genetically Modified; D011993:Recombinant Fusion Proteins / Q000737:chemistry*
chemical_list: D011993:Recombinant Fusion Proteins
keywords: EFSA; EMA; LysM; bacteria-like particles; cell-wall binding domains; genetically-modified organism; gram-positive enhancer matrix; regulatory agency; surface display