title: Molecular characterisation and prevalence of a new genotype of Cyprinid herpesvirus 2 in mainland China.
abstract: Cyprinid herpesvirus 2 (CyHV-2, species Cyprinid herpesvirus 2) has been confirmed as a causative agent of the acute haematopoietic necrosis disease outbreak in farmed goldfish (Carassius auratus L.) and gibel carp (Carassius auratus gibelio Bloch). In this study, we present the genomic characteristics of a variant CyHV-2 strain (SY-C1) isolated from farmed gibel carp in mainland China and its comparative genomics analysis with the CyHV-2 reference strain ST-J1. Overall, the full-length genome of SY-C1 shares 98.8% homology with that of ST-J1. Sequence comparisons between SY-C1 and ST-J1 indicate that the variations include single-nucleotide mutations, insertions, deletions, and rearrangements, which suggested that SY-C1 is different from ST-J1 and represents a new genotype. Therefore, we propose that the identified CyHV-2 can be divided into 2 different genotypes and be named China genotype (C genotype) and Japan genotype (J genotype) according to their isolation loci. Furthermore, epidemiological surveys indicate that the dominant genotype of CyHV-2 circulating in mainland China is closer to the China genotype than the Japan genotype.
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, People's Republic of China., Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, People's Republic of China., Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan 430070, People's Republic of China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Li
authors.forename: Yangzhi
authors.initials: Y
authors.lastname: Luo
authors.forename: Zexia
authors.initials: Z
authors.lastname: Gao
authors.forename: Jian
authors.initials: J
authors.lastname: Huang
authors.forename: Xianghai
authors.initials: X
authors.lastname: Zheng
authors.forename: Huihui
authors.initials: H
authors.lastname: Nie
authors.forename: Junmei
authors.initials: J
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Lin
authors.forename: Junfa
authors.initials: J
authors.lastname: Yuan
mesh_terms: D000818:Animals; D002681:China / Q000453:epidemiology; D005393:Fish Diseases / Q000453:epidemiology / Q000821:virology*; D005838:Genotype; D006054:Goldfish / Q000821:virology; D006564:Herpesviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D006566:Herpesviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D015995:Prevalence
keywords: Carassius auratus gibelio; Carassius auratus gibelio, Cyprinid herpesvirus 2; Cyprinid herpesvirus 2; epidemiology; genotype; goldfish; génotype; poisson rouge; épidémiologie