title: A mass spectrometric-derived cell surface protein atlas.
abstract: Cell surface proteins are major targets of biomedical research due to their utility as cellular markers and their extracellular accessibility for pharmacological intervention. However, information about the cell surface protein repertoire (the surfaceome) of individual cells is only sparsely available. Here, we applied the Cell Surface Capture (CSC) technology to 41 human and 31 mouse cell types to generate a mass-spectrometry derived Cell Surface Protein Atlas (CSPA) providing cellular surfaceome snapshots at high resolution. The CSPA is presented in form of an easy-to-navigate interactive database, a downloadable data matrix and with tools for targeted surfaceome rediscovery (http://wlab.ethz.ch/cspa). The cellular surfaceome snapshots of different cell types, including cancer cells, resulted in a combined dataset of 1492 human and 1296 mouse cell surface glycoproteins, providing experimental evidence for their cell surface expression on different cell types, including 136 G-protein coupled receptors and 75 membrane receptor tyrosine-protein kinases. Integrated analysis of the CSPA reveals that the concerted biological function of individual cell types is mainly guided by quantitative rather than qualitative surfaceome differences. The CSPA will be useful for the evaluation of drug targets, for the improved classification of cell types and for a better understanding of the surfaceome and its concerted biological functions in complex signaling microenvironments.
authors.affiliation: Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland; Department of Health Sciences and Technology, BMPP, ETH Zurich, Zurich, Switzerland.
authors.forename: Damaris
authors.initials: D
authors.lastname: Bausch-Fluck
authors.affiliation: Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland.
authors.forename: Andreas
authors.initials: A
authors.lastname: Hofmann
authors.affiliation: Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland.
authors.forename: Thomas
authors.initials: T
authors.lastname: Bock
authors.affiliation: Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland.
authors.forename: Andreas P
authors.initials: AP
authors.lastname: Frei
authors.affiliation: Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland; Laboratory of Molecular Oncology, University Hospital Zurich, Zurich, Switzerland.
authors.forename: Ferdinando
authors.initials: F
authors.lastname: Cerciello
authors.affiliation: Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland.
authors.forename: Andrea
authors.initials: A
authors.lastname: Jacobs
authors.affiliation: Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland.
authors.forename: Hansjoerg
authors.initials: H
authors.lastname: Moest
authors.affiliation: Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland; Department of Health Sciences and Technology, BMPP, ETH Zurich, Zurich, Switzerland.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Omasits
authors.affiliation: Department of Biochemistry, Medical College of Wisconsin, Wisconsin, Milwaukee, United States of America.
authors.forename: Rebekah L
authors.initials: RL
authors.lastname: Gundry
authors.affiliation: Institute for Biomaterials & Biomedical Engineering, University of Toronto, Toronto, Canada.
authors.forename: Charles
authors.initials: C
authors.lastname: Yoon
authors.affiliation: Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland.
authors.forename: Ralph
authors.initials: R
authors.lastname: Schiess
authors.affiliation: Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland.
authors.forename: Alexander
authors.initials: A
authors.lastname: Schmidt
authors.affiliation: Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland; Oncology Research Laboratory, University Children Hospital Zurich, Zurich, Switzerland.
authors.forename: Paulina
authors.initials: P
authors.lastname: Mirkowska
authors.affiliation: Centre of Advanced Studies, Faculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech Republic.
authors.forename: Anetta
authors.initials: A
authors.lastname: Härtlová
authors.affiliation: Department of Medicine, Biological Chemistry and Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America.
authors.forename: Jennifer E
authors.initials: JE
authors.lastname: Van Eyk
authors.affiliation: Oncology Research Laboratory, University Children Hospital Zurich, Zurich, Switzerland.
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Bourquin
authors.affiliation: Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland; Center for Systems Physiology and Metabolic Diseases, Zurich, Switzerland; Faculty of Science, University of Zurich, Zurich, Switzerland.
authors.forename: Ruedi
authors.initials: R
authors.lastname: Aebersold
authors.affiliation: SCRMC, LKS Faculty of Medicine, Hong Kong University, Hong Kong, Hong Kong SAR; Division of Cardiology, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America.
authors.forename: Kenneth R
authors.initials: KR
authors.lastname: Boheler
authors.affiliation: Institute for Biomaterials & Biomedical Engineering, University of Toronto, Toronto, Canada.
authors.forename: Peter
authors.initials: P
authors.lastname: Zandstra
authors.affiliation: Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland; Department of Health Sciences and Technology, BMPP, ETH Zurich, Zurich, Switzerland.
authors.forename: Bernd
authors.initials: B
authors.lastname: Wollscheid
mesh_terms: D000818:Animals; D002460:Cell Line; D030562:Databases, Protein; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008565:Membrane Proteins / Q000737:chemistry*; D051379:Mice; D040901:Proteomics / Q000379:methods*
chemical_list: D008565:Membrane Proteins