title: Butanol production from hydrothermolysis-pretreated switchgrass: Quantification of inhibitors and detoxification of hydrolyzate.
abstract: The present study evaluated butanol production from switchgrass based on hydrothermolysis pretreatment. The inhibitors present in the hydrolyzates were measured. Results showed poor butanol production (1g/L) with non-detoxified hydrolyzate. However, adjusting the pH of the non-detoxified hydrolyzate to 6 and adding 4 g/L CaCO3 increased butanol formation to about 6g/L. There was about 1g/L soluble lignin content (SLC), and various levels of furanic and phenolic compounds found in the non-detoxified hydrolyzate. Detoxification of hydrolyzates with activated carbon increased the butanol titer to 11 g/L with a total acetone, butanol and ethanol (ABE) concentration of 17 g/L. These results show the potential of butanol production from hydrothermolysis pretreated switchgrass.
authors.affiliation: Biosystems and Agricultural Engineering Department, Oklahoma State University, Stillwater, OK, USA.
authors.forename: Kan
authors.initials: K
authors.lastname: Liu
authors.affiliation: Biosystems and Agricultural Engineering Department, Oklahoma State University, Stillwater, OK, USA. Electronic address: hasan.atiyeh@okstate.edu.
authors.forename: Hasan K
authors.initials: HK
authors.lastname: Atiyeh
authors.affiliation: Biosystems and Agricultural Engineering Department, Oklahoma State University, Stillwater, OK, USA.
authors.forename: Oscar
authors.initials: O
authors.lastname: Pardo-Planas
authors.affiliation: Department of Animal Sciences, The Ohio State University, and Ohio State Agricultural Research and Development Center, Wooster, OH, USA.
authors.forename: Thaddeus C
authors.initials: TC
authors.lastname: Ezeji
authors.affiliation: Department of Animal Sciences, The Ohio State University, and Ohio State Agricultural Research and Development Center, Wooster, OH, USA.
authors.forename: Victor
authors.initials: V
authors.lastname: Ujor
authors.affiliation: Biosystems and Agricultural Engineering Department, Oklahoma State University, Stillwater, OK, USA.
authors.forename: Jonathan C
authors.initials: JC
authors.lastname: Overton
authors.affiliation: Agricultural and Biosystems Engineering Department, North Dakota State University, Fargo, ND, USA.
authors.forename: Kalli
authors.initials: K
authors.lastname: Berning
authors.affiliation: Biosystems and Agricultural Engineering Department, Oklahoma State University, Stillwater, OK, USA.
authors.forename: Mark R
authors.initials: MR
authors.lastname: Wilkins
authors.affiliation: Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK, USA.
authors.forename: Ralph S
authors.initials: RS
authors.lastname: Tanner
mesh_terms: D000096:Acetone / Q000378:metabolism; D061249:Batch Cell Culture Techniques; D001709:Biotechnology / Q000379:methods*; D000440:Butanols / Q000378:metabolism*; D002606:Charcoal / Q000494:pharmacology; D046969:Clostridium acetobutylicum / Q000254:growth & development / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation / Q000187:drug effects; D005663:Furans / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008658:Inactivation, Metabolic; D008897:Panicum / Q000187:drug effects / Q000378:metabolism*; D010636:Phenols / Q000378:metabolism; D013696:Temperature; D014867:Water
chemical_list: D000440:Butanols; D005663:Furans; D010636:Phenols; D014867:Water; D000096:Acetone; D002606:Charcoal; D000431:Ethanol; D005947:Glucose
keywords: Butanol fermentation; Clostridium acetobutylicum ATCC 824; Detoxification; Hydrothermolysis; Switchgrass