title: Role of whole bone marrow, whole bone marrow cultured cells, and mesenchymal stem cells in chronic wound healing.
abstract: BACKGROUND
  Recent evidence has shown that bone marrow cells play critical roles during the inflammatory, proliferative and remodeling phases of cutaneous wound healing. Among the bone marrow cells delivered to wounds are stem cells, which can differentiate into multiple tissue-forming cell lineages to effect, healing. Gaining insight into which lineages are most important in accelerating wound healing would be quite valuable in designing therapeutic approaches for difficult to heal wounds.
  
  
  METHODS
  In this report we compared the effect of different bone marrow preparations on established in vitro wound healing assays. The preparations examined were whole bone marrow (WBM), whole bone marrow (long term initiating/hematopoietic based) cultured cells (BMC), and bone marrow derived mesenchymal stem cells (BM-MSC). We also applied these bone marrow preparations in two murine models of radiation induced delayed wound healing to determine which had a greater effect on healing.
  
  
  RESULTS
  Angiogenesis assays demonstrated that tube formation was stimulated by both WBM and BMC, with WBM having the greatest effect. Scratch wound assays showed higher fibroblast migration at 24, 48, and 72 hours in presence of WBM as compared to BM-MSC. WBM also appeared to stimulate a greater healing response than BMC and BM-MSC in a radiation induced delayed wound healing animal model.
  
  
  CONCLUSIONS
  These studies promise to help elucidate the role of stem cells during repair of chronic wounds and reveal which cells present in bone marrow might contribute most to the wound healing process.
authors.affiliation: Department of Dermatology and Cutaneous Surgery, Leonard M. Miller School of Medicine, University of Miami, Interdisciplinary Stem Cell Institute, 1501 NW 10th Street, Suite 924, Miami, FL, 33136, USA. l.rodriguezmenocal@med.miami.edu.
authors.forename: Luis
authors.initials: L
authors.lastname: Rodriguez-Menocal
authors.affiliation: Department of Dermatology and Cutaneous Surgery, Leonard M. Miller School of Medicine, University of Miami, Interdisciplinary Stem Cell Institute, 1501 NW 10th Street, Suite 924, Miami, FL, 33136, USA. shahjahanshareef@gmail.com.
authors.forename: Shahjahan
authors.initials: S
authors.lastname: Shareef
authors.affiliation: Department of Dermatology and Cutaneous Surgery, Leonard M. Miller School of Medicine, University of Miami, Interdisciplinary Stem Cell Institute, 1501 NW 10th Street, Suite 924, Miami, FL, 33136, USA. msalgado@med.miami.edu.
authors.forename: Marcela
authors.initials: M
authors.lastname: Salgado
authors.affiliation: Department of Dermatology and Cutaneous Surgery, Leonard M. Miller School of Medicine, University of Miami, Interdisciplinary Stem Cell Institute, 1501 NW 10th Street, Suite 924, Miami, FL, 33136, USA. a.shabbir1@med.miami.edu.
authors.forename: Arsalan
authors.initials: A
authors.lastname: Shabbir
authors.affiliation: Department of Dermatology and Cutaneous Surgery, Leonard M. Miller School of Medicine, University of Miami, Interdisciplinary Stem Cell Institute, 1501 NW 10th Street, Suite 924, Miami, FL, 33136, USA. ebadiavas@med.miami.edu.
authors.forename: Evangelos
authors.initials: E
authors.lastname: Van Badiavas
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000502:physiology; D001854:Bone Marrow Cells / Q000166:cytology*; D016026:Bone Marrow Transplantation; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D004195:Disease Models, Animal; D049452:Green Fluorescent Proteins / Q000235:genetics; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018919:Neovascularization, Physiologic; D011833:Radiation Injuries, Experimental / Q000628:therapy; D014945:Wound Healing / Q000502:physiology* / Q000528:radiation effects
chemical_list: D049452:Green Fluorescent Proteins