title: Development of a nanoparticle-assisted PCR assay for detection of porcine epidemic diarrhea virus.
abstract: Porcine epidemic diarrhea virus (PEDV) is an important pig pathogen that can cause vomiting, diarrhea, and dehydration, leading to serious damage to the swine industry worldwide. In this study, a nanoparticle-assisted polymerase chain reaction (nanoPCR) assay targeting the N gene of PEDV was developed and the sensitivity and specificity were investigated. Under the optimized conditions for detection of PEDV RNA, the nanoPCR assay was 100-fold more sensitive than a conventional RT-PCR assay. The lower detection limit of the nanoPCR assay was 2.7 × 10(-6) ng/μL of PEDV RNA and no cross-reaction was observed with other viruses. This is the first report to demonstrate the application of a nanoPCR assay for the detection of PEDV. The sensitive and specific nanoPCR assay developed in this study can be applied widely in clinical diagnosis and field surveillance of PEDV-infection.
authors.affiliation: College of Animal Medicine, Agricultural University of Hebei, Baoding, Hebei 071001, China; Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding, Hebei 071001, China; North China Research Center of Animal Epidemic Pathogen Biology, China Agriculture Ministry, Baoding, Hebei 071001, China. Electronic address: yuanwanzhe@126.com.
authors.forename: Wanzhe
authors.initials: W
authors.lastname: Yuan
authors.affiliation: College of Animal Medicine, Agricultural University of Hebei, Baoding, Hebei 071001, China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Li
authors.affiliation: China Animal Disease Control Center, China Agriculture Ministry, Beijing 100125, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.affiliation: College of Animal Medicine, Agricultural University of Hebei, Baoding, Hebei 071001, China; Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding, Hebei 071001, China; North China Research Center of Animal Epidemic Pathogen Biology, China Agriculture Ministry, Baoding, Hebei 071001, China.
authors.forename: Qinye
authors.initials: Q
authors.lastname: Song
authors.affiliation: College of Animal Medicine, Agricultural University of Hebei, Baoding, Hebei 071001, China; Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding, Hebei 071001, China; North China Research Center of Animal Epidemic Pathogen Biology, China Agriculture Ministry, Baoding, Hebei 071001, China.
authors.forename: Limin
authors.initials: L
authors.lastname: Li
authors.affiliation: College of Animal Medicine, Agricultural University of Hebei, Baoding, Hebei 071001, China; Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding, Hebei 071001, China; North China Research Center of Animal Epidemic Pathogen Biology, China Agriculture Ministry, Baoding, Hebei 071001, China.
authors.forename: Jiguo
authors.initials: J
authors.lastname: Sun
mesh_terms: D000818:Animals; D018352:Coronavirus Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D017931:DNA Primers / Q000235:genetics; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D053758:Nanoparticles; D019590:Nucleocapsid Proteins / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D053485:Porcine epidemic diarrhea virus / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000821:virology; D014730:Veterinary Medicine / Q000379:methods*
chemical_list: D017931:DNA Primers; D019590:Nucleocapsid Proteins; C582965:nucleocapsid (N) protein, porcine epidemic diarrhea virus
keywords: Detection; Porcine epidemic diarrhea virus (PEDV); RT-PCR; nanoPCR