title: [Research-oriented experimental course of plant cell and gene engineering for undergraduates].
abstract: Research-oriented comprehensive experimental course for undergraduates is an important part for their training of innovation. We established an optional course of plant cell and gene engineering for undergraduates using our research platform. The course is designed to study the cellular and molecular basis and experimental techniques for plant tissue culture, isolation and culture of protoplast, genetic transformation, and screening and identification of transgenic plants. To develop undergraduates' ability in experimental design and operation, and inspire their interest in scientific research and innovation consciousness, we integrated experimental teaching and practice in plant genetic engineering on the tissue, cellular, and molecular levels. Students in the course practiced an experimental teaching model featured by two-week teaching of principles, independent experimental design and bench work, and ready-to-access laboratory. In this paper, we describe the contents, methods, evaluation system and a few issues to be solved in this course, as well as the general application and significance of the research-oriented experimental course in reforming undergraduates' teaching and training innovative talents.
authors.affiliation: School of Life Sciences, Inner Mongolia University, Hohhot 010021, China.
authors.forename: Xiao-Fei
authors.initials: XF
authors.lastname: Lin
authors.affiliation: School of Life Sciences, Inner Mongolia University, Hohhot 010021, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Zheng
authors.affiliation: School of Life Sciences, Inner Mongolia University, Hohhot 010021, China.
authors.lastname: Morigen
mesh_terms: D005818:Genetic Engineering / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007858:Learning; D008967:Molecular Biology / Q000193:education* / Q000295:instrumentation / Q000379:methods; D010944:Plants / Q000235:genetics*; D013334:Students / Q000523:psychology; D000078329:Workforce