title: Effects of Listeria monocytogenes EGD-e and Salmonella enterica ser. Typhimurium LT2 chitinases on intracellular survival in Dictyostelium discoideum and mammalian cell lines.
abstract: Some bacterial pathogens produce chitinases as virulence factors during host infection. The molecular target of such enzymes in non-chitinous hosts remains uncertain. We studied the importance of Listeria monocytogenes EGD-e and Salmonella enterica ser. Typhimurium LT2 chitinases for intracellular survival in Dictyostelium discoideum, and for Salmonella, also infection of mammalian cell lines, and a mouse model. The Salmonella chitinase did not contribute significantly to infection of D. discoideum, mammalian cell lines or mice. However, survival in D. discoideum was clearly reduced for Listeria mutants deficient of ChiB (8-fold) or deficient of both ChiA and ChiB (22-fold). Our findings suggest that chitinases from the two species play different roles in virulence.
authors.affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Grønnegårdsvej 15, 1870 Frederiksberg C, Denmark.
authors.forename: Rikki F
authors.initials: RF
authors.lastname: Frederiksen
authors.affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Grønnegårdsvej 15, 1870 Frederiksberg C, Denmark jjl@sund.ku.dk.
authors.forename: Jørgen J
authors.initials: JJ
authors.lastname: Leisner
mesh_terms: D000818:Animals; D002460:Cell Line; D002688:Chitinases / Q000235:genetics / Q000378:metabolism*; D004023:Dictyostelium / Q000382:microbiology*; D015964:Gene Expression Regulation, Bacterial; D006367:HeLa Cells; D006801:Humans; D008089:Listeria monocytogenes / Q000201:enzymology / Q000472:pathogenicity / Q000502:physiology*; D051379:Mice; D050296:Microbial Viability; D012481:Salmonella Infections, Animal / Q000382:microbiology; D012486:Salmonella typhimurium / Q000201:enzymology / Q000472:pathogenicity / Q000502:physiology*; D017384:Sequence Deletion; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D037521:Virulence Factors; D002688:Chitinases
keywords: bacterial pathogens; cellular slime moulds; glycoside hydrolase family 18; virulence factors