title: Screening of a specific peptide binding to esophageal squamous carcinoma cells from phage displayed peptide library.
abstract: To select a specifically binding peptide for imaging detection of human esophageal squamous cell carcinoma (ESCC), a phage-displayed 12-mer peptide library was used to screen the peptide that bind to ESCC cells specifically. After four rounds of bio-panning, the phage recovery rate gradually increased, and specific phage clones were effectively enriched. The 60 randomly selected phage clones were tested using cellular enzyme-linked immunosorbent assay (ELISA), and 41 phage clones were identified as positive clones with the over 2.10 ratio of absorbance higher than other clones, IRP and PBS controls. From the sequencing results of the positive clones, 14 peptide sequences were obtained and ESCP9 consensus sequence was identified as the peptide with best affinity to ESCC cells via competitive inhibition, fluorescence microscopy, and flow cytometry. The results indicate that the peptide ESCP9 can bind to ESCC cells specifically and sensitively, and it is a potential candidate to be developed as an useful molecule to the imaging detection and targeting therapy for ESCC.
authors.affiliation: Co-Innovation Center for Qinba Region's Sustainable Development, Shaanxi Normal University, Xi'an, Shaanxi 710062, China.
authors.forename: Caixia
authors.initials: C
authors.lastname: Ma
authors.affiliation: Co-Innovation Center for Qinba Region's Sustainable Development, Shaanxi Normal University, Xi'an, Shaanxi 710062, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Li
authors.affiliation: Shaanxi Railway Institute, Weinan, Shaanxi 714000, China.
authors.forename: Dongliang
authors.initials: D
authors.lastname: Jiang
authors.affiliation: College of Life and Geography Science, Qinghai Normal University, Xining, Qinghai 810008, China.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Gao
authors.affiliation: Co-Innovation Center for Qinba Region's Sustainable Development, Shaanxi Normal University, Xi'an, Shaanxi 710062, China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Han
authors.affiliation: Co-Innovation Center for Qinba Region's Sustainable Development, Shaanxi Normal University, Xi'an, Shaanxi 710062, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Xu
authors.affiliation: Co-Innovation Center for Qinba Region's Sustainable Development, Shaanxi Normal University, Xi'an, Shaanxi 710062, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Center of Medical Nanomaterial, Yulin Normal College, Yulin, Guangxi 537000, China.
authors.forename: Guochao
authors.initials: G
authors.lastname: Nie
authors.affiliation: Department of Physics and the SAVANT Center, The University of Texas at Arlington, Arlington, TX 76019, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan.
authors.forename: Fenghuei
authors.initials: F
authors.lastname: Lin
authors.affiliation: Co-Innovation Center for Qinba Region's Sustainable Development, Shaanxi Normal University, Xi'an, Shaanxi 710062, China. Electronic address: ychhou@snnu.edu.cn.
authors.forename: Yingchun
authors.initials: Y
authors.lastname: Hou
mesh_terms: D001435:Bacteriophages / Q000737:chemistry / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D004938:Esophageal Neoplasms / Q000378:metabolism* / Q000473:pathology; D000077277:Esophageal Squamous Cell Carcinoma; D057809:HEK293 Cells; D006801:Humans; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D012680:Sensitivity and Specificity
chemical_list: D019151:Peptide Library; D010455:Peptides
keywords: Esophageal squamous cell carcinoma; Molecular imaging and targeting of cancer; Peptide; Phage display peptide library