title: In vitro biocompatibility and release of curcumin from curcumin microcomplex-loaded chitosan scaffold.
abstract: BACKGROUND
  Scaffold if suitably modified could be used as a drug delivery system.
  
  
  OBJECTIVE
  To develop chitosan scaffold as a delivery system for delivering curcumin in wound-healing application.
  
  
  METHODS
  Chitosan-curcumin microcomplex particles were prepared, and the effect of drug-polymer ratio (DPR) and homogenisation speed (HS) was studied using a two-level full-factorial design. Chitosan scaffold was prepared and incorporated with curcumin microcomplexes to obtain a chitosan scaffold-containing chitosan-curcumin microcomplex (CS-CCM). Antimicrobial property of the CS-CCM against Escherichia coli was studied. The cytotoxicity of CS-CCM was studied by assessing the cell viability by MTT assay.
  
  
  CONCLUSIONS
  DPR had a significant effect (p ≤ 0.05) on the drug content. CS-CCM was able to inhibit the growth of E. coli considerably. The MTT results showed that CS-CCM is non-cytotoxic and supports cell proliferation.
  
  
  CONCLUSIONS
  CS-CCM due to its biocompatibility and antimicrobial property could be further evaluated for potential application in wound healing.
authors.affiliation: Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal University , Manipal , India .
authors.forename: Muthukumar
authors.initials: M
authors.lastname: Amirthalingam
authors.forename: Narayanan
authors.initials: N
authors.lastname: Kasinathan
authors.forename: Srinivas
authors.initials: S
authors.lastname: Mutalik
authors.forename: Nayanabhirama
authors.initials: N
authors.lastname: Udupa
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology*; D048271:Chitosan / Q000737:chemistry*; D003474:Curcumin / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology*; D004337:Drug Carriers / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D004927:Escherichia coli Infections / Q000188:drug therapy; D006801:Humans
chemical_list: D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D048271:Chitosan; D003474:Curcumin
keywords: Antimicrobial; biocompatibility; chitosan; factorial design; scaffold; wound healing