title: Effects of a granulocyte colony stimulating factor, Neulasta, in mini pigs exposed to total body proton irradiation.
abstract: Astronauts could be exposed to solar particle event (SPE) radiation, which is comprised mostly of proton radiation. Proton radiation is also a treatment option for certain cancers. Both astronauts and clinical patients exposed to ionizing radiation are at risk for loss of white blood cells (WBCs), which are the body's main defense against infection. In this report, the effect of Neulasta treatment, a granulocyte colony stimulating factor, after proton radiation exposure is discussed. Mini pigs exposed to total body proton irradiation at a dose of 2 Gy received 4 treatments of either Neulasta or saline injections. Peripheral blood cell counts and thromboelastography parameters were recorded up to 30 days post-irradiation. Neulasta significantly improved WBC loss, specifically neutrophils, in irradiated animals by approximately 60% three days after the first injection, compared to the saline treated, irradiated animals. Blood cell counts quickly decreased after the last Neulasta injection, suggesting a transient effect on WBC stimulation. Statistically significant changes in hemostasis parameters were observed after proton radiation exposure in both the saline and Neulasta treated irradiated groups, as well as internal organ complications such as pulmonary changes. In conclusion, Neulasta treatment temporarily alleviates proton radiation-induced WBC loss, but has no effect on altered hemostatic responses.
authors.forename: Jenine K
authors.initials: JK
authors.lastname: Sanzari
authors.forename: Gabriel S
authors.initials: GS
authors.lastname: Krigsfeld
authors.forename: Anne L
authors.initials: AL
authors.lastname: Shuman
authors.forename: Antonia K
authors.initials: AK
authors.lastname: Diener
authors.forename: Liyong
authors.initials: L
authors.lastname: Lin
authors.forename: Wilfried
authors.initials: W
authors.lastname: Mai
authors.forename: Ann R
authors.initials: AR
authors.lastname: Kennedy
mesh_terms: D000818:Animals; D004906:Erythrocyte Count; D004912:Erythrocytes / Q000187:drug effects*; D000069585:Filgrastim; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D007958:Leukocyte Count; D009504:Neutrophils / Q000187:drug effects*; D011092:Polyethylene Glycols; D011522:Protons / Q000009:adverse effects; D011839:Radiation, Ionizing; D011994:Recombinant Proteins / Q000494:pharmacology; D019445:Solar Activity; D013552:Swine; D013556:Swine, Miniature; D013916:Thrombelastography / Q000187:drug effects*; D014916:Whole-Body Irradiation / Q000009:adverse effects*
chemical_list: D011522:Protons; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; C455861:pegfilgrastim; D011092:Polyethylene Glycols; D000069585:Filgrastim