title: Low mir-372 expression correlates with poor prognosis and tumor metastasis in hepatocellular carcinoma.
abstract: BACKGROUND
  Recent studies have shown that miR-372 plays important roles in hepatocellular carcinoma (HCC) progression. However, results have been conflicting regarding its expression levels and role in HCC.
  
  
  METHODS
  RT-PCR and in situ hybridization was used to evaluate miR-372 expression in HCC tissues and cell lines. The methylation status of neighboring CpG islands upstream of the miR-372 promoter was analyzed by methylation-specific PCR (MSP). Transfection of miR-372 mimic into HCC cell lines was used to evaluate cellular proliferation and invasion. Prognostic significance was analyzed by the Kaplan-Meier survival method and Cox regression.
  
  
  RESULTS
  miR-372 was expressed at lower levels in HCC tissues compared with controls and was related to tumor metastasis and poor prognosis. Hypermethylation of miR-372 was detected in HCC cell lines and tissues, and miR-372 expression was restored upon 5-aza-dCyd treatment. Upregulated expression by mir-372 mimic transfection inhibited proliferation and invasion capacity in HCC cells.
  
  
  CONCLUSIONS
  miR-372 may play an important role in hepatic carcinogenesis and may serve as a new target or method to detect and treat HCC in the future.
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning, 110001, China. wgzwl@hotmail.com.
authors.forename: Gang
authors.initials: G
authors.lastname: Wu
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning, 110001, China. 619643092@qq.com.
authors.forename: Yawei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Changhai Hospital Affiliated to Second Military Medical University, Shanghai, China. 460532253@qq.com.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Lu
authors.affiliation: Department of General Surgery, The First Hospital Affiliated to Dalian Medical University, Dalian, China. 104330298@qq.com.
authors.forename: Hui
authors.initials: H
authors.lastname: He
authors.affiliation: Department of General Surgery, The Fourth Hospital Affiliated to China Medical University, Shenyang, Liaoning, China. 283558710@qq.com.
authors.forename: Haiyang
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning, 110001, China. 414297222@qq.com.
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Meng
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning, 110001, China. 247508163@qq.com.
authors.forename: Shuguan
authors.initials: S
authors.lastname: Xia
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning, 110001, China. 812559949@qq.com.
authors.forename: Kunming
authors.initials: K
authors.lastname: Zheng
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning, 110001, China. 18660232387@163.com.
authors.forename: Boqian
authors.initials: B
authors.lastname: Liu
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000401:mortality / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D018899:CpG Islands; D019175:DNA Methylation; D005260:Female; D005500:Follow-Up Studies; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008113:Liver Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D009362:Neoplasm Metastasis; D011379:Prognosis; D011401:Promoter Regions, Genetic
chemical_list: C561021:MIRN372 microRNA, human; D035683:MicroRNAs