title: The probiotic mixture IRT5 ameliorates age-dependent colitis in rats.
abstract: To investigate the anti-inflammatory effect of probiotics, we orally administered IRT5 (1×10(9)CFU/rat) for 8 weeks to aged (16 months-old) Fischer 344 rats, and measured parameters of colitis. The expression levels of the inflammatory markers' inducible NO synthase (iNOS), cyclooxygenase-2 (COX2), tumor necrosis factor (TNF)-α, and interleukin (IL)-1β were higher in the colons of normal aged rats (18 months-old) than in the colons of normal young rats (6 months-old). Treatment with IRT5 suppressed the age-associated increased expression of iNOS, COX2, TNF-α, and IL-1β, and activation of NF-κB and mitogen-activated protein kinases. In a similar manner, the expression of tight junction proteins in the colon of normal aged rats was suppressed more potently than in normal young rats, and treatment of aged rats with IRT5 decreased the age-dependent suppression of tight junction proteins ZO-1, occludin, and claudin-1. Treatment with IRT5 suppressed age-associated increases in expressions of senescence markers p16 and p53 in the colon of aged rats, but increased age-suppressed expression of SIRT1. However, treatment with IRT5 inhibited age-associated increased myeloperoxidase activity in the colon. In addition, treatment with IRT5 lowered the levels of LPS in intestinal fluid and blood of aged rats, as well as the reduced concentrations of reactive oxygen species, malondialdehyde, and C-reactive protein in the blood. These findings suggest that IRT5 treatment may suppress age-dependent colitis by inhibiting gut microbiota LPS production.
authors.affiliation: Department of Life and Nanopharmaceutical Sciences, College of Pharmacy, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Jin-Ju
authors.initials: JJ
authors.lastname: Jeong
authors.affiliation: Department of Life and Nanopharmaceutical Sciences, College of Pharmacy, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Jae-Yeon
authors.initials: JY
authors.lastname: Woo
authors.affiliation: R&B D Center, Korea Yakult Co., Ltd., Yongin 449-901, Republic of Korea.
authors.forename: Young-Tae
authors.initials: YT
authors.lastname: Ahn
authors.affiliation: R&B D Center, Korea Yakult Co., Ltd., Yongin 449-901, Republic of Korea.
authors.forename: Jae-Hun
authors.initials: JH
authors.lastname: Shim
authors.affiliation: Graduate School of International Agricultured Technology, GBST, Seoul National University, 232-961, Gangwon, Republic of Korea.
authors.forename: Chul-Sung
authors.initials: CS
authors.lastname: Huh
authors.affiliation: Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea.
authors.forename: Sin-Heog
authors.initials: SH
authors.lastname: Im
authors.affiliation: Department of Food and Nutrition, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Myung Joo
authors.initials: MJ
authors.lastname: Han
authors.affiliation: Department of Life and Nanopharmaceutical Sciences, College of Pharmacy, Kyung Hee University, Seoul 130-701, Republic of Korea. Electronic address: dhkim@khu.ac.kr.
authors.forename: Dong-Hyun
authors.initials: DH
authors.lastname: Kim
mesh_terms: D000375:Aging / Q000378:metabolism; D000818:Animals; D062445:Claudin-1 / Q000235:genetics / Q000378:metabolism; D003092:Colitis / Q000382:microbiology / Q000517:prevention & control*; D003106:Colon / Q000187:drug effects* / Q000473:pathology; D045424:Complex Mixtures / Q000008:administration & dosage*; D051546:Cyclooxygenase 2 / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D000069196:Gastrointestinal Microbiome / Q000187:drug effects*; D018836:Inflammation Mediators / Q000378:metabolism; D008297:Male; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D062793:Occludin / Q000235:genetics / Q000378:metabolism; D019936:Probiotics / Q000008:administration & dosage*; D051381:Rats; D011919:Rats, Inbred Strains; D017382:Reactive Oxygen Species / Q000378:metabolism; D056564:Sirtuin 1 / Q000235:genetics / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism; D062826:Zonula Occludens-1 Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: D062445:Claudin-1; D045424:Complex Mixtures; D016207:Cytokines; D018836:Inflammation Mediators; D062793:Occludin; D017382:Reactive Oxygen Species; C569044:Tjp1 protein, rat; D016159:Tumor Suppressor Protein p53; D062826:Zonula Occludens-1 Protein; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2; C488368:Sirt1 protein, rat; D056564:Sirtuin 1
keywords: Aging; Colitis; IRT5; Inflammaging; Probiotics