title: [(13)C3,(15)N1]-labeled isodesmosine: A potential internal standard for LC-MS/MS analysis of desmosines in elastin degradation.
abstract: Isodesmosine and desmosine are crosslinking amino acids that are present only in elastin. They are useful biomarkers for the degradation of elastin, which occurs during the progression of chronic obstructive pulmonary disease (COPD) and related diseases. This Letter describes the synthesis of [(13)C3,(15)N1]-labeled isodesmosine, using Chichibabin pyridine synthesis as a key reaction. The labeled isodesmosine is a potential internal standard for the quantitative LC-MS/MS analysis of desmosines in elastin degradation.
authors.affiliation: Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, 7-1 Kioicho, Chiyoda-ku, Tokyo 102-8554, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Tanigawa
authors.affiliation: Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, 7-1 Kioicho, Chiyoda-ku, Tokyo 102-8554, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Komatsu
authors.affiliation: Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, 7-1 Kioicho, Chiyoda-ku, Tokyo 102-8554, Japan. Electronic address: t-usuki@sophia.ac.jp.
authors.forename: Toyonobu
authors.initials: T
authors.lastname: Usuki
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D002247:Carbon Isotopes / Q000032:analysis; D002853:Chromatography, Liquid; D003895:Desmosine / Q000032:analysis*; D004549:Elastin / Q000737:chemistry / Q000378:metabolism*; D007524:Isodesmosine / Q000138:chemical synthesis* / Q000737:chemistry; D015394:Molecular Structure; D009587:Nitrogen Isotopes / Q000032:analysis; D029424:Pulmonary Disease, Chronic Obstructive / Q000473:pathology; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers; D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D003895:Desmosine; D004549:Elastin; D007524:Isodesmosine
keywords: Biomarker; Chichibabin pyridine synthesis; Elastin degradation; Isodesmosine; Isotopic label