title: ﻿﻿﻿Mixed Suspension of Cultured Autologous and Allogenic Keratinocytes in Fibrin Glue for the Treatment of Full-thickness Burns.
abstract: ﻿﻿﻿﻿﻿ ﻿﻿﻿ ﻿Abstract: In-vitro cultured autologous and allogenic keratinocytes were mixed in single-cell suspension and transplanted for the treatment of full-thickness, third-degree burns. Ten patients were selected according to a protocol approved by the authors' local Institutional Review Board. After epifascial necrectomy, the burns were temporarily covered with fresh allografts, and a mixed suspension of in-vitro cultured autologous and allogenic keratinocytes in fibrin glue with a density of 107 cells/mL was transplanted to the wounds by injection with a syringe in multipoint underneath the allograft. The grafting density was 5 × 106 cells per 100 cm2. The transplanted keratinocytes proliferated on the epifascial surface and grew into confluent epithelial layer 28 ± 7 days after transplantation. Complete re-epithelialization and stable skin conditions were achieved 62 ± 11 days after transplantation. This method decreased in-vitro cell culture time to 5 days and resulted in permanent re-epithelialization of third-degree burn wounds. To the authors' knowledge, this is the first report where autologous and allogenic keratinocytes were mixed and transplanted together to treat third-degree burn wounds. The performance and density of the keratinocytes remain to be studied ﻿﻿﻿.
authors.affiliation: ﻿
authors.forename: Guozhong
authors.initials: G
authors.lastname: Lu
authors.forename: Liangliang
authors.initials: L
authors.lastname: Cai
authors.affiliation: Wuxi Third Renmin Hospital, China; Email: lausee@126.com.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
authors.forename: Zaiqiu
authors.initials: Z
authors.lastname: Gu
authors.forename: Yugang
authors.initials: Y
authors.lastname: Zhu