title: Generation and evaluation of a recombinant genotype VII Newcastle disease virus expressing VP3 protein of Goose parvovirus as a bivalent vaccine in goslings.
abstract: Newcastle disease virus (NDV) and Goose parvovirus (GPV) are considered to be two of the most important and widespread viruses infecting geese. In this study, we generated a recombinant rmNA-VP3, expressing GPV VP3 using a modified goose-origin NDV NA-1 by changing the multi-basic cleavage site motif RRQKR↓F of the F protein to the dibasic motif GRQGR↓L as that of the avirulent strain LaSota as a vaccine vector. Expression of the VP3 protein in rmNA-VP3 infected cells was detected by immunofluorescence and Western blot assay. The genetic stability was examined by serially passaging 10 times in 10-day-old embryonated SPF chicken eggs. Goslings were inoculated with rmNA-VP3 showed no apparent signs of disease and developed a strong GPV and NDV neutralizing antibodies response. This is the first study demonstrating that recombinant NDV has the potential to serve as bivalent live vaccine against Goose parvovirus and Newcastle disease virus infection in birds.
authors.affiliation: Laboratory of Infectious Diseases, College of Veterinary Medicine, Jilin University, Changchun 130062, China; Key Laboratory of Zoonosis Research, Ministry of Education, Jilin University, Changchun 130062, China.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Wang
authors.affiliation: Laboratory of Infectious Diseases, College of Veterinary Medicine, Jilin University, Changchun 130062, China; Key Laboratory of Zoonosis Research, Ministry of Education, Jilin University, Changchun 130062, China.
authors.forename: Yanlong
authors.initials: Y
authors.lastname: Cong
authors.affiliation: Laboratory of Infectious Diseases, College of Veterinary Medicine, Jilin University, Changchun 130062, China; Key Laboratory of Zoonosis Research, Ministry of Education, Jilin University, Changchun 130062, China.
authors.forename: Renfu
authors.initials: R
authors.lastname: Yin
authors.affiliation: Institute of Military Veterinary Medicine, Academy of Military Medical Science, Changchun 130122, China.
authors.forename: Na
authors.initials: N
authors.lastname: Feng
authors.affiliation: Institute of Military Veterinary Medicine, Academy of Military Medical Science, Changchun 130122, China.
authors.forename: Songtao
authors.initials: S
authors.lastname: Yang
authors.affiliation: Institute of Military Veterinary Medicine, Academy of Military Medical Science, Changchun 130122, China.
authors.forename: Xianzhu
authors.initials: X
authors.lastname: Xia
authors.affiliation: Shandong Binzhou Animal Science and Veterinary Medicine Institute, Binzhou 256600, China.
authors.forename: Yueqiang
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Shandong Binzhou Animal Science and Veterinary Medicine Institute, Binzhou 256600, China.
authors.forename: Wenxiu
authors.initials: W
authors.lastname: Wang
authors.affiliation: Animal Infectious Disease Laboratory, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China.
authors.forename: Xiufan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Animal Infectious Disease Laboratory, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China.
authors.forename: Shunlin
authors.initials: S
authors.lastname: Hu
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.
authors.forename: Chan
authors.initials: C
authors.lastname: Ding
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.
authors.forename: Shengqing
authors.initials: S
authors.lastname: Yu
authors.affiliation: Engineering Research Center of Jilin Province for Animals Probiotics, College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China. Electronic address: wangchunfeng@jlau.edu.cn.
authors.forename: Chunfeng
authors.initials: C
authors.lastname: Wang
authors.affiliation: Laboratory of Infectious Diseases, College of Veterinary Medicine, Jilin University, Changchun 130062, China; Key Laboratory of Zoonosis Research, Ministry of Education, Jilin University, Changchun 130062, China. Electronic address: dingzhuang@jlu.edu.cn.
authors.forename: Zhuang
authors.initials: Z
authors.lastname: Ding
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D005777:Geese; D042822:Genomic Instability; D009521:Newcastle Disease / Q000517:prevention & control*; D009522:Newcastle disease virus / Q000235:genetics / Q000276:immunology*; D010322:Parvoviridae Infections / Q000517:prevention & control / Q000662:veterinary*; D019219:Parvovirinae / Q000235:genetics / Q000276:immunology*; D011201:Poultry Diseases / Q000517:prevention & control*; D012692:Serial Passage; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D014614:Vaccines, Synthetic; D014765:Viral Vaccines
keywords: Attenuated; Goose parvovirus; Recombinant Newcastle disease virus; VP3; Vectored vaccine