title: Polymorphisms of the IL-6, IL-8 and IL-10 genes and the risk of gastric pathology in patients infected with Helicobacter pylori.
abstract: OBJECTIVE
  Helicobacter pylori-induced gastric mucosal inflammation is mediated by proinflammatory and anti-inflammatory cytokines. Polymorphisms in genes that code cytokines influence cytokine secretion levels and appear to contribute to the risk of gastric diseases. In this sense, we performed this study to identify the polymorphisms in the IL-6, IL-8, and IL-10 genes and their associations with H. pylori infection and gastric pathologies.
  
  
  METHODS
  Gastric biopsy samples of 151 patients infected with H. pylori and 76 uninfected individuals were used. Helicobacter pylori infection was diagnosed by histological examination and the detection of the ureA and glmM genes. The polymorphisms in the IL-6 (at position -174), IL-8 (at position -251), and IL-10 (at position -819) were detected by polymerase chain reaction-restriction fragment length polymorphism.
  
  
  RESULTS
  Among the genetic polymorphisms studied, we observed that only the presence of the A allele at position -251 of the IL-8 gene was significantly associated with H. pylori infection. In addition, patient carriers of the A/A genotype at position -251 of the IL-8 gene and carriers of the T allele at position -819 of the IL-10 gene had an increased risk of peptic ulcer disease in the presence of H. pylori infection. We did not find a correlation between polymorphisms in the IL-6, IL-8, and IL-10 genes and a higher risk of gastric carcinoma.
  
  
  CONCLUSIONS
  We demonstrated that polymorphisms in the IL-8 gene was significantly associated with H. pylori infection. Furthermore, polymorphisms in the IL-8 and IL-10 genes were associated with an enhanced risk of peptic ulcer disease in H. pylori-positive patients.
authors.affiliation: Centro de Desenvolvimento Tecnológico-Universidade Federal de Pelotas (UFPel), Pelotas, Rio Grande do Sul, Brazil. Electronic address: ivynha_@hotmail.com.
authors.forename: Ivy Bastos
authors.initials: IB
authors.lastname: Ramis
authors.affiliation: Centro de Desenvolvimento Tecnológico-Universidade Federal de Pelotas (UFPel), Pelotas, Rio Grande do Sul, Brazil.
authors.forename: Júlia Silveira
authors.initials: JS
authors.lastname: Vianna
authors.affiliation: Núcleo de Pesquisa em Microbiologia Médica-Universidade Federal do Rio Grande (FURG), Rio Grande, Rio Grande do Sul, Brazil.
authors.forename: Carla Vitola
authors.initials: CV
authors.lastname: Gonçalves
authors.affiliation: Núcleo de Pesquisa em Microbiologia Médica-Universidade Federal do Rio Grande (FURG), Rio Grande, Rio Grande do Sul, Brazil.
authors.forename: Andrea
authors.initials: A
authors.lastname: von Groll
authors.affiliation: Centro de Desenvolvimento Tecnológico-Universidade Federal de Pelotas (UFPel), Pelotas, Rio Grande do Sul, Brazil.
authors.forename: Odir Antônio
authors.initials: OA
authors.lastname: Dellagostin
authors.affiliation: Centro de Desenvolvimento Tecnológico-Universidade Federal de Pelotas (UFPel), Pelotas, Rio Grande do Sul, Brazil; Núcleo de Pesquisa em Microbiologia Médica-Universidade Federal do Rio Grande (FURG), Rio Grande, Rio Grande do Sul, Brazil.
authors.forename: Pedro Eduardo Almeida
authors.initials: PEA
authors.lastname: da Silva
mesh_terms: D001706:Biopsy; D005260:Female; D005753:Gastric Mucosa / Q000382:microbiology / Q000473:pathology*; D020022:Genetic Predisposition to Disease; D005838:Genotype; D016481:Helicobacter Infections / Q000235:genetics* / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D016480:Helicobacter pylori / Q000302:isolation & purification*; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics; D016209:Interleukin-8 / Q000235:genetics; D007378:Interleukins / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012307:Risk Factors; D013274:Stomach Neoplasms / Q000235:genetics / Q000382:microbiology / Q000473:pathology
chemical_list: C508609:IL10 protein, human; D015850:Interleukin-6; D016209:Interleukin-8; D007378:Interleukins; D016753:Interleukin-10
keywords: Genetic polymorphism; Helicobacter pylori; Interleukin-10 gene; Interleukin-6 gene; Interleukin-8 gene