title: An Arabidopsis thaliana copper-sensitive mutant suggests a role of phytosulfokine in ethylene production.
abstract: To increase our understanding of the adaptation for copper (Cu) deficiency, Arabidopsis mutants with apparent alterations under Cu deficiency were identified. In this report, a novel mutant, tpst-2, was found to be more sensitive than wild-type (Col-0) plants to Cu deficiency during root elongation. The positional cloning of tpst-2 revealed that this gene encodes a tyrosylprotein sulfotransferase (TPST). Moreover, the ethylene production of tpst-2 mutant was higher than that of Col-0 under Cu deficiency, and adding the ethylene response inhibitor AgNO3 partially rescued defects in root elongation. Interestingly, peptide hormone phytosulfokine (PSK) treatment also repressed the ethylene production of tpst-2 mutant plants. Our results revealed that TPST suppressed ethylene production through the action of PSK.
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region, Ministry of Agriculture), Horticultural College, Northeast Agricultural University, 59 Mucai Street, Harbin 150030, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wu
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Takehiro
authors.initials: T
authors.lastname: Kamiya
authors.affiliation: National Agriculture and Food Research Organization, Institute of Floricultural Science, 3-1-1 Kannondai, Tsukuba, Ibaraki 305-8666, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Yumoto
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Sotta
authors.affiliation: National Institute for Basic Biology, Okazaki 444-8585, Japan.
authors.forename: Yamaguchi
authors.initials: Y
authors.lastname: Katsushi
authors.affiliation: National Institute for Basic Biology, Okazaki 444-8585, Japan.
authors.forename: Shuji
authors.initials: S
authors.lastname: Shigenobu
authors.affiliation: Division of Biological Science, Graduate School of Science, Nagoya Universisy, Chikusa-ku, Nagoya 464-8602, Japan.
authors.forename: Yoshikatsu
authors.initials: Y
authors.lastname: Matsubayashi
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan atorufu@mail.ecc.u-tokyo.ac.jp.
authors.forename: Toru
authors.initials: T
authors.lastname: Fujiwara
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D003300:Copper / Q000494:pharmacology*; D005030:Ethylenes / Q000096:biosynthesis*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D006706:Homeostasis / Q000187:drug effects; D009154:Mutation / Q000235:genetics*; D010641:Phenotype; D064209:Phytochemicals / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics; D012326:RNA Splicing / Q000235:genetics; D011956:Receptors, Cell Surface / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D012835:Silver Nitrate / Q000494:pharmacology; D015238:Sulfotransferases / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D005030:Ethylenes; D064209:Phytochemicals; D011956:Receptors, Cell Surface; D003300:Copper; C036216:ethylene; D012835:Silver Nitrate; D015238:Sulfotransferases; C039079:protein-tyrosine sulfotransferase
keywords: Cu; PSK; TPST.; deficiency; ethylene; root elongation