title: The expression of TLR-9, CD86, and CD95 phenotypes in circulating B cells of patients with chronic viral hepatitis B or C before and after antiviral therapy.
abstract: OBJECTIVE
  This study aimed to assess the differential expression of specific B cell subtypes in patients with chronic viral hepatitis.
  
  
  METHODS
  The frequencies of differential expression of specific B cell subtypes in patients with chronic viral hepatitis and healthy controls were assessed by flow cytometry using monoclonal antibodies specific for CD38, CD27, CD86, CD95, TLR-9, and IgD. The effect of adefovir treatment on B cell subsets in HBV patients was determined. The values of clinical parameters in the patients were also measured.
  
  
  RESULTS
  The frequency of CD86+ B cells was not significantly different in chronic HBV patients but was higher in HCV patients compared with that in healthy controls. CD95 and IgD levels were lower in HBV and HCV patients than in healthy controls. A significant negative correlation occurred between the proportion of CD95+ B cells and HBV DNA viral load. The frequency of TLR-9 on the B cells in HBV and HCV patients was higher compared with that of healthy controls. After treatment with adefovir, the frequency of CD95 and IgD expressed on B cells was increased in HBV patients.
  
  
  CONCLUSIONS
  Activated B cells and exhausted B cells homeostasis were commonly disturbed in HBV and HCV patients.
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, The First Hospital, Jilin University, Changchun 130021, China.
authors.forename: Ping-Wei
authors.initials: PW
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, The First Hospital, Jilin University, Changchun 130021, China ; Department of Gastroenterology, The First People's Hospital of Changzhou, Third Affiliated Hospital of Suzhou University, Changzhou, Jiangsu 213003, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Hepatology Disease, The First Hospital, Jilin University, Changchun 130021, China.
authors.forename: Hui-Fan
authors.initials: HF
authors.lastname: Ji
authors.affiliation: Department of Infectious Disease, The Fourth Hospital of Harbin Medical University, Harbin 150001, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yu
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, The First Hospital, Jilin University, Changchun 130021, China.
authors.forename: Jun-Yan
authors.initials: JY
authors.lastname: Feng
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, The First Hospital, Jilin University, Changchun 130021, China.
authors.forename: Juan
authors.identifier: 0000-0002-7822-2661
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, The First Hospital, Jilin University, Changchun 130021, China ; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.
authors.forename: Ming-Yuan
authors.initials: MY
authors.lastname: Liu
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, The First Hospital, Jilin University, Changchun 130021, China ; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.
authors.forename: Yan-Fang
authors.initials: YF
authors.lastname: Jiang
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives / Q000627:therapeutic use; D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal / Q000737:chemistry; D000998:Antiviral Agents / Q000627:therapeutic use; D001402:B-Lymphocytes / Q000821:virology; D051940:B7-2 Antigen / Q000097:blood*; D004279:DNA, Viral / Q000097:blood; D005260:Female; D005434:Flow Cytometry; D005838:Genotype; D019694:Hepatitis B, Chronic / Q000097:blood* / Q000188:drug therapy*; D019698:Hepatitis C, Chronic / Q000097:blood* / Q000188:drug therapy*; D006706:Homeostasis; D006801:Humans; D007072:Immunoglobulin D / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008297:Male; D008875:Middle Aged; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D063065:Organophosphonates / Q000627:therapeutic use; D010641:Phenotype; D015995:Prevalence; D051217:Toll-Like Receptor 9 / Q000097:blood*; D019562:Viral Load; D055815:Young Adult; D019014:fas Receptor / Q000097:blood*
chemical_list: D000911:Antibodies, Monoclonal; D000998:Antiviral Agents; D051940:B7-2 Antigen; C497964:CD86 protein, human; C408982:CPG-oligonucleotide; D004279:DNA, Viral; C491901:FAS protein, human; D007072:Immunoglobulin D; D009838:Oligodeoxyribonucleotides; D063065:Organophosphonates; C495371:TLR9 protein, human; D051217:Toll-Like Receptor 9; D019014:fas Receptor; D000225:Adenine; C106812:adefovir dipivoxil