title: In-vitro model for studying methanogens in human gut microbiota.
abstract: Reported failures with gnotobiotic animal models led us to establish an in-vitro model of reciprocal conversion of methanogenic and non methanogenic microbiota from human fecal samples. Consequences on gas and microbiota compositions are reported. This should facilitate the study of the controversial role of gut methanogens in human health.
authors.affiliation: EA-4678 CIDAM, Clermont Université, Université d'Auvergne, Clermont-Ferrand, France.
authors.forename: William
authors.initials: W
authors.lastname: Tottey
authors.affiliation: EA-4678 CIDAM, Clermont Université, Université d'Auvergne, Clermont-Ferrand, France.
authors.forename: Nadia
authors.initials: N
authors.lastname: Gaci
authors.affiliation: School of Microbiology and Alimentary Pharmabiotic Centre, University College Cork, Ireland.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Borrel
authors.affiliation: EA-4678 CIDAM, Clermont Université, Université d'Auvergne, Clermont-Ferrand, France.
authors.forename: Monique
authors.initials: M
authors.lastname: Alric
authors.affiliation: School of Microbiology and Alimentary Pharmabiotic Centre, University College Cork, Ireland.
authors.forename: Paul W
authors.initials: PW
authors.lastname: O'Toole
authors.affiliation: EA-4678 CIDAM, Clermont Université, Université d'Auvergne, Clermont-Ferrand, France. Electronic address: jf.brugere@udamail.fr.
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Brugère
mesh_terms: D000069196:Gastrointestinal Microbiome; D006801:Humans; D008697:Methane / Q000378:metabolism*; D008828:Microbiological Techniques / Q000379:methods*; D064307:Microbiota; D008962:Models, Theoretical
chemical_list: D008697:Methane
keywords: Colon; ECSIM; Gut microbiota; Human gut archaea; In vitro systems; Methanobrevibacter smithii