title: Identification of new genes contributing to the extreme radioresistance of Deinococcus radiodurans using a Tn5-based transposon mutant library.
abstract: Here, we have developed an extremely efficient in vivo Tn5-based mutagenesis procedure to construct a Deinococcus radiodurans insertion mutant library subsequently screened for sensitivity to genotoxic agents such as γ and UV radiations or mitomycin C. The genes inactivated in radiosensitive mutants belong to various functional categories, including DNA repair functions, stress responses, signal transduction, membrane transport, several metabolic pathways, and genes of unknown function. Interestingly, preliminary characterization of previously undescribed radiosensitive mutants suggests the contribution of cyclic di-AMP signaling in the recovery of D. radiodurans cells from genotoxic stresses, probably by modulating several pathways involved in the overall cell response. Our analyses also point out a new transcriptional regulator belonging to the GntR family, encoded by DR0265, and a predicted RNase belonging to the newly described Y family, both contributing to the extreme radioresistance of D. radiodurans. Altogether, this work has revealed new cell responses involved either directly or indirectly in repair of various cell damage and confirmed that D. radiodurans extreme radiation resistance is determined by a multiplicity of pathways acting as a complex network.
authors.affiliation: Univ. Paris-Sud, Institute for Integrative Biology of the Cell (I2BC), Université Paris Saclay, CEA, CNRS, Orsay, France.
authors.forename: Rémi
authors.initials: R
authors.lastname: Dulermo
authors.affiliation: Univ. Paris-Sud, Institute for Integrative Biology of the Cell (I2BC), Université Paris Saclay, CEA, CNRS, Orsay, France.
authors.forename: Takefumi
authors.initials: T
authors.lastname: Onodera
authors.affiliation: Univ. Paris-Sud, Institute for Integrative Biology of the Cell (I2BC), Université Paris Saclay, CEA, CNRS, Orsay, France.
authors.forename: Geneviève
authors.initials: G
authors.lastname: Coste
authors.affiliation: Univ. Paris-Sud, Institute for Integrative Biology of the Cell (I2BC), Université Paris Saclay, CEA, CNRS, Orsay, France.
authors.forename: Fanny
authors.initials: F
authors.lastname: Passot
authors.affiliation: Univ. Paris-Sud, Institute for Integrative Biology of the Cell (I2BC), Université Paris Saclay, CEA, CNRS, Orsay, France.
authors.forename: Murielle
authors.initials: M
authors.lastname: Dutertre
authors.affiliation: Univ. Paris-Sud, Institute for Integrative Biology of the Cell (I2BC), Université Paris Saclay, CEA, CNRS, Orsay, France.
authors.forename: Martine
authors.initials: M
authors.lastname: Porteron
authors.affiliation: Univ. Paris-Sud, Institute for Integrative Biology of the Cell (I2BC), Université Paris Saclay, CEA, CNRS, Orsay, France.
authors.forename: Fabrice
authors.initials: F
authors.lastname: Confalonieri
authors.affiliation: Univ. Paris-Sud, Institute for Integrative Biology of the Cell (I2BC), Université Paris Saclay, CEA, CNRS, Orsay, France.
authors.forename: Suzanne
authors.initials: S
authors.lastname: Sommer
authors.affiliation: Univ. Paris-Sud, Institute for Integrative Biology of the Cell (I2BC), Université Paris Saclay, CEA, CNRS, Orsay, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Pasternak
mesh_terms: D001426:Bacterial Proteins / Q000145:classification / Q000235:genetics / Q000502:physiology; D004249:DNA Damage; D004260:DNA Repair / Q000235:genetics; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D034301:Deinococcus / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D015226:Dinucleoside Phosphates / Q000502:physiology; D005720:Gamma Rays; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D015723:Gene Library; D053263:Gene Regulatory Networks; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D006861:Hydrogen Peroxide / Q000494:pharmacology; D016685:Mitomycin / Q000494:pharmacology; D016254:Mutagenesis, Insertional; D009154:Mutation; D016366:Open Reading Frames / Q000235:genetics; D018384:Oxidative Stress; D011836:Radiation Tolerance / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000302:isolation & purification; D019895:Transposases / Q000235:genetics; D014466:Ultraviolet Rays
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D015226:Dinucleoside Phosphates; D014157:Transcription Factors; C528998:cyclic diadenosine phosphate; D016685:Mitomycin; D006861:Hydrogen Peroxide; D019895:Transposases