title: Trichostrongylus colubriformis: Possible Most Common Cause of Human Infection in Mazandaran Province, North of Iran.
abstract: BACKGROUND
  Infection with Trichostrongylus spp. is common among human and herbivorous in most parts of Iran, especially in southern and northern areas. The aim of present study was to identify Trichostrongylus spp. among human population using excreted egg specimens, by the molecular method, in Mazandaran Province, northern Iran.
  
  
  METHODS
  Overall, 33 positive fecal specimens were randomly sampled and examined. PCR amplification of ITS2-rDNA region was performed on the isolated egg and then a restriction fragment length polymorphism (RFLP) profile was considered to discriminate of Trichostrongylus spp.
  
  
  RESULTS
  A total of 33 positive fecal specimens, 29(78.9%), 4(12.1%) were found T. colubriformis and T. axei respectively. Our data appear the molecular evidence of both human T. colubriformis and T. axei infections in North of Iran.
  
  
  CONCLUSIONS
  T. colubriformis was the probable most common zoonotic species causing human trichostrongylosis infection in the area.
authors.affiliation: Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Shirzad
authors.initials: S
authors.lastname: Gholami
authors.affiliation: Antimicrobial Resistance Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Farhang
authors.initials: F
authors.lastname: Babamahmoodi
authors.affiliation: Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Rohallah
authors.initials: R
authors.lastname: Abedian
authors.affiliation: Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Sharif
authors.affiliation: Research Center of Infectious and Tropical Diseases, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Shahbazi
authors.affiliation: Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Abdolsattar
authors.initials: A
authors.lastname: Pagheh
authors.affiliation: Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Fakhar
keywords: Human; ITS2-PCR; Iran; Trichostrongylus axei; Trichostrongylus colubriformis