title: Study of vortex-assisted MSPD and LC-MS/MS using alternative solid supports for pharmaceutical extraction from marketed fish.
abstract: A procedure based on vortex-assisted matrix solid-phase dispersion (MSPD) for the extraction of 15 pharmaceuticals from fish samples with determination by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was validated. Florisil, C18, diatomaceous earth, chitin, and chitosan were evaluated as solid supports. Best results were obtained with 0.5 g of diatomaceous earth, 0.5 g of sodium sulfate, and 5 mL of methanol. Analytical recoveries ranged from 58 to 128 % with relative standard deviation (RSD) lower than 15 %. Limit of quantification (LOQ) values for the 15 compounds ranged from 5 to 1000 ng g(-1). The method under investigation has shown to be a simple and fast extraction tool with minimum instrumentation and low amount of reagent, resulting in method low cost. Besides, alternative materials, such as chitin and chitosan, which were applied to the dispersion step for the first time, were found to be interesting alternatives.
authors.affiliation: Post-graduate Program in Technological and Environmental Chemistry, Escola de Química e Alimentos, Laboratório de Análise de Compostos Orgânicos e Metais (LACOM), Universidade Federal do Rio Grande, Av. Itália, km 8, Rio Grande, Rio Grande do Sul state, 96203-900, Brazil.
authors.forename: Gabriel I
authors.initials: GI
authors.lastname: Hertzog
authors.forename: Karina L
authors.initials: KL
authors.lastname: Soares
authors.forename: Sergiane S
authors.initials: SS
authors.lastname: Caldas
authors.forename: Ednei G
authors.initials: EG
authors.lastname: Primel
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D005399:Fishes; D057230:Limit of Detection; D004364:Pharmaceutical Preparations / Q000302:isolation & purification*; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004364:Pharmaceutical Preparations