title: Immunohistochemical characterisation of parasitic pneumonias of dolphins stranded in the Canary Islands.
abstract: This is the first work that applies immunohistochemistry in the characterisation of the inflammatory infiltrate of verminous bronchopneumonia associated with lungworm parasites in stranded dolphins. Samples from three different species (Stenella coerulealba, Stenella frontalis and Delphinus delphis) stranded in the Canary Islands were used. The most common findings found in these animals varied from bronchitis to verminous bronchopneumonia with different degree of severity. The immunohistochemical study showed variable expressions of Lysozyme, MHC-II, iNOS and IgG. The main population presenting in the inflammatory infiltrates were CD3(+) lymphocytes. However, moderate number of Foxp3(+) lymphocytes was found in lymph nodes even though no Foxp3(+) cells were found in lung lesions in any of the samples analysed. This study revealed that lung lesions showed a chronic inflammatory infiltrate mainly composed by lymphocytes CD3(+). Deeper studies are needed in order to provide a more complete scope about the infiltrates involved in these types of lesions.
authors.affiliation: Unit of Veterinary Histology and Pathology, Institute for Animal Health (IUSA), Veterinary School, University of Las Palmas de Gran Canaria, Transmontaña s/n 35416, Arucas (Gran Canaria), Spain.
authors.forename: R
authors.initials: R
authors.lastname: Zafra
authors.affiliation: Unit of Veterinary Histology and Pathology, Institute for Animal Health (IUSA), Veterinary School, University of Las Palmas de Gran Canaria, Transmontaña s/n 35416, Arucas (Gran Canaria), Spain.
authors.forename: J R
authors.initials: JR
authors.lastname: Jaber
authors.affiliation: Department of Anatomy and Comparative Pathology. Veterinary Faculty, University of Córdoba, Animal Health Building, Campus de Rabanales, Ctra. Madrid-Cádiz km. 396, 14014 Córdoba, Spain. Electronic address: an1pearj@uco.es.
authors.forename: J
authors.initials: J
authors.lastname: Pérez
authors.affiliation: Unit of Veterinary Histology and Pathology, Institute for Animal Health (IUSA), Veterinary School, University of Las Palmas de Gran Canaria, Transmontaña s/n 35416, Arucas (Gran Canaria), Spain.
authors.forename: J
authors.initials: J
authors.lastname: de la Fuente
authors.affiliation: Unit of Veterinary Histology and Pathology, Institute for Animal Health (IUSA), Veterinary School, University of Las Palmas de Gran Canaria, Transmontaña s/n 35416, Arucas (Gran Canaria), Spain.
authors.forename: M
authors.initials: M
authors.lastname: Arbelo
authors.affiliation: Unit of Veterinary Histology and Pathology, Institute for Animal Health (IUSA), Veterinary School, University of Las Palmas de Gran Canaria, Transmontaña s/n 35416, Arucas (Gran Canaria), Spain.
authors.forename: M
authors.initials: M
authors.lastname: Andrada
authors.affiliation: Unit of Veterinary Histology and Pathology, Institute for Animal Health (IUSA), Veterinary School, University of Las Palmas de Gran Canaria, Transmontaña s/n 35416, Arucas (Gran Canaria), Spain.
authors.forename: A
authors.initials: A
authors.lastname: Fernández
mesh_terms: D000818:Animals; D050696:Common Dolphins; D005260:Female; D007150:Immunohistochemistry / Q000662:veterinary; D008297:Male; D008681:Metastrongyloidea / Q000302:isolation & purification*; D011014:Pneumonia / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*; D013030:Spain; D050706:Stenella; D017206:Strongylida Infections / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*
keywords: Dolphin; Immunohistochemistry; Lung; Parasite