title: Metformin potentiates rapamycin and cisplatin in gastric cancer in mice.
abstract: Here we showed that pAMPKα and PTEN were down-regulated and p-mTOR, p-S6, p-4EBP1, MMP7, and DCN1 were up-regulated in human gastric cancer tissue samples as compared to that in the noncancerous tissues. Metformin inhibited tumor growth in mice. Also it enhanced cisplatin- or rapamycin-induced reduction of tumor growth as compared with treatment of either drug alone. In addition to activation of AMPK and suppression of the mTOR pathway, a series of increased and decreased genes expression were induced by metformin, including PTEN, MMP7, and FN1. We suggest that metformin could potentially be used for the treatment of gastric cancer especially in combination with cisplatin or rapamycin.
authors.affiliation: Department of Medical Oncology, Changzheng Hospital, Shanghai 200070, China.|Department of Neurosurgery, The University of Texas MD Anderson Cancer Center, Houston, Texas 77030, USA.
authors.forename: Guanzhen
authors.initials: G
authors.lastname: Yu
authors.affiliation: Department of Medical Oncology, Changzheng Hospital, Shanghai 200070, China.|Department of Oncology, Fuzhou General Hospital, Clinical Medical College of Fujian Medical University, Fuzhou, Fujian 350025, China.
authors.forename: Wenzheng
authors.initials: W
authors.lastname: Fang
authors.affiliation: Department of Gastroenterology, Changhai Hospital, Shanghai 200433, China.|Department of Gastroenterology, Hepatology & Nutrition, The University of Texas MD Anderson Cancer Center, Houston, Texas 77030, USA.
authors.forename: Tian
authors.initials: T
authors.lastname: Xia
authors.affiliation: Department of Pathology, Changhai Hospital, Shanghai 200433, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Oncology, Qingdao, Shandong 266000, China.
authors.forename: Yunshu
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Medical Oncology, Changzheng Hospital, Shanghai 200070, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Jiao
authors.affiliation: Department of Neurosurgery, The University of Texas MD Anderson Cancer Center, Houston, Texas 77030, USA.
authors.forename: Suyun
authors.initials: S
authors.lastname: Huang
authors.affiliation: Department of Medical Oncology, Changzheng Hospital, Shanghai 200070, China.
authors.forename: Jiejun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, Changhai Hospital, Shanghai 200433, China.
authors.forename: Zhaosheng
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Gastroenterology, Hepatology & Nutrition, The University of Texas MD Anderson Cancer Center, Houston, Texas 77030, USA.
authors.forename: Keping
authors.initials: K
authors.lastname: Xie
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use*; D015153:Blotting, Western; D002453:Cell Cycle / Q000187:drug effects; D002945:Cisplatin / Q000008:administration & dosage; D004195:Disease Models, Animal; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008687:Metformin / Q000008:administration & dosage; D051379:Mice; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects; D020123:Sirolimus / Q000008:administration & dosage; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000473:pathology; D059467:Transcriptome / Q000187:drug effects*; D023041:Xenograft Model Antitumor Assays
chemical_list: D008687:Metformin; D002945:Cisplatin; D020123:Sirolimus
keywords: experimental therapy; gastric cancer; gene expression; metformin; mouse model