title: Complete Genome Sequence of Bifidobacterium longum GT15: Identification and Characterization of Unique and Global Regulatory Genes.
abstract: In this study, we report the first completely annotated genome sequence of the Russia origin Bifidobacterium longum subsp. longum strain GT15. Comparative genomic analysis of this genome with other available completely annotated genome sequences of B. longum strains isolated from other countries has revealed a high degree of conservation and synteny across the entire genomes. However, it was discovered that the open reading frames to 35 genes were detected only from the B. longum GT15 genome and absent from other genomes B. longum strains (not of Russian origin). These so-called unique genes (UGs) represent a total length of 39,066 bp, with G + C content ranging from 37 to 65 %. Interestingly, certain genes were detected in other B. longum strains of Russian origin. In our analysis, we examined genes for global regulatory systems: proteins of toxin-antitoxin (TA) systems type II, serine/threonine protein kinases (STPKs) of eukaryotic type, and genes of the WhiB-like family proteins. In addition, we have made in silico analysis of all the most significant probiotic genes and considered genes involved in epigenetic regulation and genes responsible for producing various neuromediators. This genome sequence may elucidate the biology of this probiotic strain as a promising candidate for practical (pharmaceutical) applications.
authors.affiliation: Vavilov Institute of General Genetics, Gubkina str. 3, 119991, Moscow, Russia. zakharevich@yandex.ru.
authors.forename: Natalia V
authors.initials: NV
authors.lastname: Zakharevich
authors.affiliation: Vavilov Institute of General Genetics, Gubkina str. 3, 119991, Moscow, Russia.
authors.forename: Olga V
authors.initials: OV
authors.lastname: Averina
authors.affiliation: Vavilov Institute of General Genetics, Gubkina str. 3, 119991, Moscow, Russia.
authors.forename: Ksenia M
authors.initials: KM
authors.lastname: Klimina
authors.affiliation: Engelhardt Institute of Molecular Biology, Vavilova str. 32, 119991, Moscow, Russia.
authors.forename: Anna V
authors.initials: AV
authors.lastname: Kudryavtseva
authors.affiliation: Vavilov Institute of General Genetics, Gubkina str. 3, 119991, Moscow, Russia.
authors.forename: Artem S
authors.initials: AS
authors.lastname: Kasianov
authors.affiliation: Vavilov Institute of General Genetics, Gubkina str. 3, 119991, Moscow, Russia.|Engelhardt Institute of Molecular Biology, Vavilova str. 32, 119991, Moscow, Russia.
authors.forename: Vsevolod J
authors.initials: VJ
authors.lastname: Makeev
authors.affiliation: Vavilov Institute of General Genetics, Gubkina str. 3, 119991, Moscow, Russia.
authors.forename: Valery N
authors.initials: VN
authors.lastname: Danilenko
mesh_terms: D001644:Bifidobacterium / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000235:genetics* / Q000378:metabolism; D044127:Epigenesis, Genetic; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D010802:Phylogeny; D012426:Russia; D017422:Sequence Analysis, DNA
keywords: Bifidobacteria; Genome sequencing; Probiotic genes; Stress response genes; Unique genes