title: A Time-Encoded Technique for fibre-based hyperspectral broadband stimulated Raman microscopy.
abstract: Raman sensing and microscopy are among the most specific optical technologies to identify the chemical compounds of unknown samples, and to enable label-free biomedical imaging. Here we present a method for stimulated Raman scattering spectroscopy and imaging with a time-encoded (TICO) Raman concept. We use continuous wave, rapidly wavelength-swept probe lasers and combine them with a short-duty-cycle actively modulated pump laser. Hence, we achieve high stimulated Raman gain signal levels, while still benefitting from the narrow linewidth and low noise of continuous wave operation. Our all-fibre TICO-Raman setup uses a Fourier domain mode-locked laser source to achieve a unique combination of high speed, broad spectral coverage (750-3,150 cm(-1)) and high resolution (0.5 cm(-1)). The Raman information is directly encoded and acquired in time. We demonstrate quantitative chemical analysis of a solvent mixture and hyperspectral Raman microscopy with molecular contrast of plant cells.
authors.affiliation: Lehrstuhl für BioMolekulare Optik, Fakultät für Physik, Ludwig-Maximilians-Universität München, Oettingenstreet 67, 80538 Munich, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Karpf
authors.affiliation: Lehrstuhl für BioMolekulare Optik, Fakultät für Physik, Ludwig-Maximilians-Universität München, Oettingenstreet 67, 80538 Munich, Germany.|Institut für Biomedizinische Optik, Universität zu Lübeck, Peter-Monnik-Weg 4, 23562 Lübeck, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Eibl
authors.affiliation: Lehrstuhl für BioMolekulare Optik, Fakultät für Physik, Ludwig-Maximilians-Universität München, Oettingenstreet 67, 80538 Munich, Germany.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Wieser
authors.affiliation: Lehrstuhl für BioMolekulare Optik, Fakultät für Physik, Ludwig-Maximilians-Universität München, Oettingenstreet 67, 80538 Munich, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Klein
authors.affiliation: Lehrstuhl für BioMolekulare Optik, Fakultät für Physik, Ludwig-Maximilians-Universität München, Oettingenstreet 67, 80538 Munich, Germany.|Institut für Biomedizinische Optik, Universität zu Lübeck, Peter-Monnik-Weg 4, 23562 Lübeck, Germany.
authors.forename: Robert
authors.initials: R
authors.lastname: Huber
mesh_terms: D001554:Benzene / Q000032:analysis; D003510:Cyclohexanes / Q000032:analysis; D005583:Fourier Analysis; D031315:Geranium; D018547:Plant Stems / Q000648:ultrastructure*; D012996:Solutions / Q000032:analysis / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013997:Time Factors; D014050:Toluene / Q000032:analysis
chemical_list: D003510:Cyclohexanes; D012996:Solutions; D014050:Toluene; C506365:Cyclohexane; D001554:Benzene