title: Phenylpropanoids and flavonoids from Phlomis kurdica as inhibitors of human lactate dehydrogenase.
abstract: Two flavonoids, jaceosidin 7-O-β-d-glucopyranosyl-(1→2)-β-d-glucopyranoside (1) and hispidulin 7-O-β-d-glucopyranosyl-(1→2)-β-d-glucopyranoside (2), and one phenylpropanoid, 3,3'-dimethyl-lunariifolioside (3), along with 11 known compounds (4-14), were isolated from the aerial parts of Phlomis kurdica growing in Jordan. Structures of 1-3 were elucidated on the basis of spectroscopic data. These isolated compounds were assayed for their inhibitory activity against isoform 5 of human lactate dehydrogenase. Compound 4, luteolin 7-O-β-d-glucopyranoside, showed an IC50 value comparable to that of galloflavin, used as reference compound. Docking studies were carried out to hypothesize the interaction mode of compound 4 in the enzyme active site.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Umm Al-Qura University, P.O. Box 13174, 21955 Makkah, Saudi Arabia.
authors.forename: Ammar
authors.initials: A
authors.lastname: Bader
authors.affiliation: Dipartimento di Farmacia, Università di Pisa, via Bonanno 6 and 33, 56126 Pisa, Italy; Centro Interdipartimentale di Ricerca "Nutraceutica e Alimentazione per la Salute", Università di Pisa, via del Borghetto 80, 56124 Pisa, Italy.
authors.forename: Tiziano
authors.initials: T
authors.lastname: Tuccinardi
authors.affiliation: Dipartimento di Farmacia, Università di Pisa, via Bonanno 6 and 33, 56126 Pisa, Italy.
authors.forename: Carlotta
authors.initials: C
authors.lastname: Granchi
authors.affiliation: Dipartimento di Farmacia, Università di Pisa, via Bonanno 6 and 33, 56126 Pisa, Italy; Centro Interdipartimentale di Ricerca "Nutraceutica e Alimentazione per la Salute", Università di Pisa, via del Borghetto 80, 56124 Pisa, Italy.
authors.forename: Adriano
authors.initials: A
authors.lastname: Martinelli
authors.affiliation: Dipartimento di Farmacia, Università di Pisa, via Bonanno 6 and 33, 56126 Pisa, Italy; Centro Interdipartimentale di Ricerca "Nutraceutica e Alimentazione per la Salute", Università di Pisa, via del Borghetto 80, 56124 Pisa, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Macchia
authors.affiliation: Dipartimento di Farmacia, Università di Pisa, via Bonanno 6 and 33, 56126 Pisa, Italy; Centro Interdipartimentale di Ricerca "Nutraceutica e Alimentazione per la Salute", Università di Pisa, via del Borghetto 80, 56124 Pisa, Italy.
authors.forename: Filippo
authors.initials: F
authors.lastname: Minutolo
authors.affiliation: Dipartimento di Farmacia, Università degli Studi di Salerno, via Giovanni Paolo II 132, 84084 Fisciano, SA, Italy. Electronic address: detommasi@unisa.it.
authors.forename: Nunziatina
authors.initials: N
authors.lastname: De Tommasi
authors.affiliation: Dipartimento di Farmacia, Università di Pisa, via Bonanno 6 and 33, 56126 Pisa, Italy; Centro Interdipartimentale di Ricerca "Nutraceutica e Alimentazione per la Salute", Università di Pisa, via del Borghetto 80, 56124 Pisa, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Braca
mesh_terms: D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D006801:Humans; D007597:Jordan; D007770:L-Lactate Dehydrogenase / Q000037:antagonists & inhibitors*; D015394:Molecular Structure; D010666:Phenylpropionates / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D031345:Phlomis / Q000737:chemistry*; D035261:Plant Components, Aerial / Q000737:chemistry
chemical_list: D005419:Flavonoids; D010666:Phenylpropionates; C477508:jaceosidin; D007770:L-Lactate Dehydrogenase
keywords: Flavonoids; LDH inhibitors; Lamiaceae; Phenolics; Phlomis kurdica