title: Magnetogenetic control of protein gradients inside living cells with high spatial and temporal resolution.
abstract: Tools for controlling the spatial organization of proteins are a major prerequisite for deciphering mechanisms governing the dynamic architecture of living cells. Here, we have developed a generic approach for inducing and maintaining protein gradients inside living cells by means of biofunctionalized magnetic nanoparticles (MNPs). For this purpose, we tailored the size and surface properties of MNPs in order to ensure unhindered mobility in the cytosol. These MNPs with a core diameter below 50 nm could be rapidly relocalized in living cells by exploiting biased diffusion at weak magnetic forces in the femto-Newton range. In combination with MNP surface functionalization for specific in situ capturing of target proteins as well as efficient delivery into the cytosplasm, we here present a comprehensive technology for controlling intracellular protein gradients with a temporal resolution of a few tens of seconds.
authors.affiliation: †Laboratoire Physico-Chimie, Institut Curie, CNRS UMR168, Paris-Science Lettres, Université Pierre et Marie Curie-Paris 6, 75005 Paris, France.
authors.forename: Fred
authors.initials: F
authors.lastname: Etoc
authors.affiliation: †Laboratoire Physico-Chimie, Institut Curie, CNRS UMR168, Paris-Science Lettres, Université Pierre et Marie Curie-Paris 6, 75005 Paris, France.
authors.forename: Chiara
authors.initials: C
authors.lastname: Vicario
authors.affiliation: †Laboratoire Physico-Chimie, Institut Curie, CNRS UMR168, Paris-Science Lettres, Université Pierre et Marie Curie-Paris 6, 75005 Paris, France.|‡Department of Biology, University of Osnabrück, 49076 Osnabrück, Germany.
authors.forename: Domenik
authors.initials: D
authors.lastname: Lisse
authors.affiliation: §Sorbonne Universités, UPMC Univ Paris 06, UMR 8234, PHENIX, F-75005 Paris, France.
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Siaugue
authors.affiliation: ‡Department of Biology, University of Osnabrück, 49076 Osnabrück, Germany.
authors.forename: Jacob
authors.initials: J
authors.lastname: Piehler
authors.affiliation: †Laboratoire Physico-Chimie, Institut Curie, CNRS UMR168, Paris-Science Lettres, Université Pierre et Marie Curie-Paris 6, 75005 Paris, France.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Coppey
authors.affiliation: †Laboratoire Physico-Chimie, Institut Curie, CNRS UMR168, Paris-Science Lettres, Université Pierre et Marie Curie-Paris 6, 75005 Paris, France.
authors.forename: Maxime
authors.initials: M
authors.lastname: Dahan
mesh_terms: D002460:Cell Line; D003600:Cytosol / Q000737:chemistry / Q000187:drug effects*; D006801:Humans; D058185:Magnetite Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D010316:Particle Size; D011506:Proteins / Q000008:administration & dosage / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D058185:Magnetite Nanoparticles; D011506:Proteins
keywords: Magnetogenetics; cell signaling; intracellular diffusion; nanomagnetism; nanoparticles; protein manipulation