title: Cyclic voltammetric acetylcholinesterase biosensor for the detection of captan in apple samples with the aid of chemometrics.
abstract: The presence of captan residues in apples shows high toxicity, which often causes eye and skin irritation, dermatitis, conjunctivitis, and vomiting in humans. In this context, an electrochemical biosensor based on acetylcholinesterase (AChE) immobilized on a ZnO nanorod interface has been proposed. In this work, Hill, dose-response, and first-, second-, and third-order polynomial regression models were successfully applied and the prediction ability of these models was tested with the use of current density obtained from the cyclic voltammograms of appropriate captan solutions. The Pt/ZnO/AChE bioelectrode showed a high sensitivity of 0.538 μA cm(-2) μM(-1) in the linear range from 0.05 to 25.0 μM with a limit of detection of 107 nM. The recovery results were observed between 98.4 and 102.4 % from the apple sample. This work provides a new promising tool for the detection of captan in apple samples.
authors.affiliation: Nanosensors Lab, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India.
authors.forename: Noel
authors.initials: N
authors.lastname: Nesakumar
authors.forename: Swaminathan
authors.initials: S
authors.lastname: Sethuraman
authors.forename: Uma Maheswari
authors.initials: UM
authors.lastname: Krishnan
authors.forename: John Bosco Balaguru
authors.initials: JB
authors.lastname: Rayappan
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry*; D015374:Biosensing Techniques; D002215:Captan / Q000032:analysis*; D055664:Electrochemical Techniques; D057230:Limit of Detection
chemical_list: D000110:Acetylcholinesterase; D002215:Captan