title: Inhibitory effect of acteoside on melittin-induced catecholamine exocytosis through inhibition of Ca(2+)-dependent phospholipase A2 and extracellular Ca(2+) influx in PC12 cells.
abstract: To investigate the inhibitory effect of acteoside on the process of exocytosis induced by melittin, we measured Ca(2+) mobilization, arachidonic acid (AA) release and catecholamine exocytosis in PC12 chromaffin cells. Melittin significantly increased the intracellular Ca(2+) mobilization via receptor-operated calcium channel but not the intracellular Ca(2+) release. It caused AA release via activation of Ca(2+)-dependent phospholipase A2 (PLA2) and catecholamine secretion in a dose-dependent manner. Acteoside dose-dependently inhibited the release of AA and intracellular Ca(2+) mobilization induced by melittin. Acteoside reduced the catecholamine release and raised the amount of intracellular chromogranin A which is co-released with catecholamine from melittin-stimulated PC12 cells. Taken together, our results suggest that acteoside could suppress the exocytosis via inhibition of Ca(2+)-dependent PLA2 and extracellular Ca(2+) influx in PC12 cells stimulated by melittin.
authors.affiliation: College of Pharmacy, Chung-Ang University, 221 Huksuk-dong, Dongjak-Gu, Seoul, 156-756, Korea.
authors.forename: Ho Sun
authors.initials: HS
authors.lastname: Song
authors.affiliation: College of Pharmacy, Chung-Ang University, 221 Huksuk-dong, Dongjak-Gu, Seoul, 156-756, Korea.
authors.forename: Myung Soo
authors.initials: MS
authors.lastname: Ko
authors.affiliation: College of Pharmacy, Chung-Ang University, 221 Huksuk-dong, Dongjak-Gu, Seoul, 156-756, Korea.
authors.forename: Young Soo
authors.initials: YS
authors.lastname: Jo
authors.affiliation: College of Pharmacy, Chung-Ang University, 221 Huksuk-dong, Dongjak-Gu, Seoul, 156-756, Korea.
authors.forename: Wan Kyunn
authors.initials: WK
authors.lastname: Whang
authors.affiliation: College of Pharmacy, Chung-Ang University, 221 Huksuk-dong, Dongjak-Gu, Seoul, 156-756, Korea. simss@cau.ac.kr.
authors.forename: Sang Soo
authors.initials: SS
authors.lastname: Sim
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000378:metabolism; D002395:Catecholamines / Q000378:metabolism; D053379:Chromogranin A / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005089:Exocytosis / Q000187:drug effects*; D005960:Glucosides / Q000008:administration & dosage / Q000494:pharmacology*; D008555:Melitten / Q000494:pharmacology*; D016716:PC12 Cells; D010636:Phenols / Q000008:administration & dosage / Q000494:pharmacology*; D054467:Phospholipases A2 / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D059808:Polyphenols
chemical_list: D016718:Arachidonic Acid; D002118:Calcium; D015220:Calcium Channels; D002395:Catecholamines; D053379:Chromogranin A; D005960:Glucosides; D008555:Melitten; D010636:Phenols; D054467:Phospholipases A2; C058956:acteoside; D059808:Polyphenols
keywords: Acteoside; Arachidonic acid; Ca2+ mobilization; Catecholamine; Chromogranin A; Phospholipase A2