title: Functional expression of TRPV channels in T cells and their implications in immune regulation.
abstract: The importance of Ca(2+) signalling and temperature in the context of T cell activation is well known. However, the molecular identities of key players involved in such critical regulations are still unknown. In this work we explored the endogenous expression of transient receptor potential vanilloid (TRPV) channels, a group of thermosensitive and non-selective cation channels, in T cells. Using flow cytometry and confocal microscopy, we demonstrate that members belonging to the TRPV subfamily are expressed endogenously in the human T cell line Jurkat, in primary human T cells and in primary murine splenic T cells. We also demonstrate that TRPV1- and TRPV4-specific agonists, namely resiniferatoxin and 4α-phorbol-12,13-didecanoate, can cause Ca(2+) influx in T cells. Moreover, our results show that expression of these channels can be upregulated in T cells during concanavalin A-driven mitogenic and anti-CD3/CD28 stimulated TCR activation of T cells. By specific blocking of TRPV1 and TRPV4 channels, we found that these TRPV inhibitors may regulate mitogenic and T cell receptor mediated T cell activation and effector cytokine(s) production by suppressing tumour necrosis factor, interleukin-2 and interferon-γ release. These results may have broad implications in the context of cell-mediated immunity, especially T cell responses and their regulations, neuro-immune interactions and molecular understanding of channelopathies.
authors.affiliation: School of Biological Sciences, National Institute of Science Education and Research, Bhubaneswar, Orissa, India.
authors.forename: Rakesh K
authors.initials: RK
authors.lastname: Majhi
authors.affiliation: School of Biological Sciences, National Institute of Science Education and Research, Bhubaneswar, Orissa, India.
authors.forename: Subhransu S
authors.initials: SS
authors.lastname: Sahoo
authors.affiliation: School of Biological Sciences, National Institute of Science Education and Research, Bhubaneswar, Orissa, India.
authors.forename: Manoj
authors.initials: M
authors.lastname: Yadav
authors.affiliation: School of Biological Sciences, National Institute of Science Education and Research, Bhubaneswar, Orissa, India.
authors.forename: Belluru M
authors.initials: BM
authors.lastname: Pratheek
authors.affiliation: School of Biological Sciences, National Institute of Science Education and Research, Bhubaneswar, Orissa, India.
authors.forename: Subhasis
authors.initials: S
authors.lastname: Chattopadhyay
authors.affiliation: School of Biological Sciences, National Institute of Science Education and Research, Bhubaneswar, Orissa, India.
authors.forename: Chandan
authors.initials: C
authors.lastname: Goswami
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002118:Calcium / Q000378:metabolism; D006801:Humans; D019169:Jurkat Cells; D008213:Lymphocyte Activation; D008297:Male; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D013154:Spleen / Q000166:cytology / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D050916:TRPV Cation Channels / Q000276:immunology* / Q000378:metabolism*
chemical_list: D050916:TRPV Cation Channels; C494482:TRPV1 protein, human; C400769:TRPV2 protein, human; C459445:TRPV3 protein, human; C416986:TRPV4 protein, human; D002118:Calcium
keywords: Ca2+ influx; T cell receptor; T cells; TRPV channels; immune activation and effector function; neuro-immune interaction