title: Differential gene expression in foxtail millet during incompatible interaction with Uromyces setariae-italicae.
abstract: Foxtail millet (Setaria italica) is an important food and fodder grain crop that is grown for human consumption. Production of this species is affected by several plant diseases, such as rust. The cultivar Shilixiang has been identified as resistant to the foxtail millet rust pathogen, Uromyces setariae-italicae. In order to identify signaling pathways and genes related to the plant's defense mechanisms against rust, the Shilixiang cultivar was used to construct a digital gene expression (DGE) library during the interaction of foxtail millet with U. setariae-italicae. In this study, we determined the most abundant differentially expressed signaling pathways of up-regulated genes in foxtail millet and identified significantly up-regulated genes. Finally, quantitative real-time polymerase chain reaction (qRT-PCR) analysis was used to analyze the expression of nine selected genes, and the patterns observed agreed well with DGE analysis. Expression levels of the genes were also compared between a resistant cultivar Shilixiang and a susceptible cultivar Yugu-1, and the result indicated that expression level of Shilixiang is higher than that of Yugu-1. This study reveals the relatively comprehensive mechanisms of rust-responsive transcription in foxtail millet.
authors.affiliation: Department of plant protect, Millet Institute, Hebei Academy of Agricultural and Forestry Sciences, National Foxtail Millet Improvement Center, Minor Cereal Crops Laboratory of Hebei Province, Shijiazhuang, China.
authors.forename: Zhi Yong
authors.initials: ZY
authors.lastname: Li
authors.affiliation: Department of plant protect, Millet Institute, Hebei Academy of Agricultural and Forestry Sciences, National Foxtail Millet Improvement Center, Minor Cereal Crops Laboratory of Hebei Province, Shijiazhuang, China; College of Life Science, Hebei Normal University, Shijiazhuang, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.affiliation: Department of plant protect, Millet Institute, Hebei Academy of Agricultural and Forestry Sciences, National Foxtail Millet Improvement Center, Minor Cereal Crops Laboratory of Hebei Province, Shijiazhuang, China.
authors.forename: Li
authors.initials: L
authors.lastname: Dong
authors.affiliation: Department of plant protect, Millet Institute, Hebei Academy of Agricultural and Forestry Sciences, National Foxtail Millet Improvement Center, Minor Cereal Crops Laboratory of Hebei Province, Shijiazhuang, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Bai
authors.affiliation: Department of plant protect, Millet Institute, Hebei Academy of Agricultural and Forestry Sciences, National Foxtail Millet Improvement Center, Minor Cereal Crops Laboratory of Hebei Province, Shijiazhuang, China.
authors.forename: Jian Zhang
authors.initials: JZ
authors.lastname: Quan
authors.affiliation: Department of plant protect, Millet Institute, Hebei Academy of Agricultural and Forestry Sciences, National Foxtail Millet Improvement Center, Minor Cereal Crops Laboratory of Hebei Province, Shijiazhuang, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of plant protect, Millet Institute, Hebei Academy of Agricultural and Forestry Sciences, National Foxtail Millet Improvement Center, Minor Cereal Crops Laboratory of Hebei Province, Shijiazhuang, China.
authors.forename: Zhi-Ping
authors.initials: ZP
authors.lastname: Dong
mesh_terms: D018506:Gene Expression Regulation, Plant; D010935:Plant Diseases / Q000235:genetics*; D032983:Setaria Plant / Q000235:genetics*