title: Fungal Infection Increases the Rate of Somatic Mutation in Scots Pine (Pinus sylvestris L.).
abstract: Somatic mutations are transmitted during mitosis in developing somatic tissue. Somatic cells bearing the mutations can develop into reproductive (germ) cells and the somatic mutations are then passed on to the next generation of plants. Somatic mutations are a source of variation essential to evolve new defense strategies and adapt to the environment. Stem rust disease in Scots pine has a negative effect on wood quality, and thus adversely affects the economy. It is caused by the 2 most destructive fungal species in Scandinavia: Peridermium pini and Cronartium flaccidum. We studied nuclear genome stability in Scots pine under biotic stress (fungus-infected, 22 trees) compared to a control population (plantation, 20 trees). Stability was assessed as accumulation of new somatic mutations in 10 microsatellite loci selected for genotyping. Microsatellites are widely used as molecular markers in population genetics studies of plants, and are particularly used for detection of somatic mutations as their rate of mutation is of a much higher magnitude when compared with other DNA markers. We report double the rate of somatic mutation per locus in the fungus-infected trees (4.8×10(-3) mutations per locus), as compared to the controls (2.0×10(-3) mutations per locus) when individual samples were analyzed at 10 different microsatellite markers. Pearson's chi-squared test indicated a significant effect of the fungal infection which increased the number of mutations in the fungus-infected trees (χ(2) = 12.9883, df = 1, P = 0.0003134).
authors.affiliation: From the Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre (UPSC), Swedish University of Agricultural Sciences, SE 901-83 Umeå, Sweden (Ranade, Ganea, Razzak, and García Gil).
authors.forename: Sonali Sachin
authors.initials: SS
authors.lastname: Ranade
authors.affiliation: From the Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre (UPSC), Swedish University of Agricultural Sciences, SE 901-83 Umeå, Sweden (Ranade, Ganea, Razzak, and García Gil).
authors.forename: Laura-Stefana
authors.initials: LS
authors.lastname: Ganea
authors.affiliation: From the Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre (UPSC), Swedish University of Agricultural Sciences, SE 901-83 Umeå, Sweden (Ranade, Ganea, Razzak, and García Gil).
authors.forename: Abdur M
authors.initials: AM
authors.lastname: Razzak
authors.affiliation: From the Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre (UPSC), Swedish University of Agricultural Sciences, SE 901-83 Umeå, Sweden (Ranade, Ganea, Razzak, and García Gil). M.Rosario.Garcia@slu.se.
authors.forename: M R
authors.initials: MR
authors.lastname: García Gil
mesh_terms: D001487:Basidiomycota / Q000472:pathogenicity*; D018744:DNA, Plant / Q000235:genetics; D005819:Genetic Markers; D005828:Genetics, Population; D018745:Genome, Plant; D042822:Genomic Instability; D005838:Genotype; D054884:Host-Pathogen Interactions / Q000235:genetics*; D018895:Microsatellite Repeats; D009154:Mutation; D059645:Mutation Rate; D041605:Pinus sylvestris / Q000235:genetics* / Q000382:microbiology; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013548:Sweden
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers
keywords: Scots pine; Somatic mutation.; abiotic stress; microsatellite; simple sequence repeats