title: Comparative analysis of chrysanthemum transcriptome in response to three RNA viruses: Cucumber mosaic virus, Tomato spotted wilt virus and Potato virus X.
abstract: The chrysanthemum is one of popular flowers in the world and a host for several viruses. So far, molecular interaction studies between the chrysanthemum and viruses are limited. In this study, we carried out a transcriptome analysis of chrysanthemum in response to three different viruses including Cucumber mosaic virus (CMV), Tomato spotted wilt virus (TSWV) and Potato virus X (PVX). A chrysanthemum 135K microarray derived from expressed sequence tags was successfully applied for the expression profiles of the chrysanthemum at early stage of virus infection. Finally, we identified a total of 125, 70 and 124 differentially expressed genes (DEGs) for CMV, TSWV and PVX, respectively. Many DEGs were virus specific; however, 33 DEGs were commonly regulated by three viruses. Gene ontology (GO) enrichment analysis identified a total of 132 GO terms, and of them, six GO terms related stress response and MCM complex were commonly identified for three viruses. Several genes functioning in stress response such as chitin response and ethylene mediated signaling pathway were up-regulated indicating their involvement in establishment of host immune system. In particular, TSWV infection significantly down-regulated genes related to DNA metabolic process including DNA replication, chromatin organization, histone modification and cytokinesis, and they are mostly targeted to nucleosome and MCM complex. Taken together, our comparative transcriptome analysis revealed several genes related to hormone mediated viral stress response and DNA modification. The identified chrysanthemums genes could be good candidates for further functional study associated with resistant to various plant viruses.
authors.affiliation: Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Republic of Korea.
authors.forename: Hoseong
authors.initials: H
authors.lastname: Choi
authors.forename: Yeonhwa
authors.initials: Y
authors.lastname: Jo
authors.forename: Sen
authors.initials: S
authors.lastname: Lian
authors.forename: Kyoung-Min
authors.initials: KM
authors.lastname: Jo
authors.forename: Hyosub
authors.initials: H
authors.lastname: Chu
authors.forename: Ju-Yeon
authors.initials: JY
authors.lastname: Yoon
authors.forename: Seung-Kook
authors.initials: SK
authors.lastname: Choi
authors.forename: Kook-Hyung
authors.initials: KH
authors.lastname: Kim
authors.forename: Won Kyong
authors.initials: WK
authors.lastname: Cho
mesh_terms: D002736:Chloroplasts / Q000235:genetics; D031202:Chrysanthemum / Q000235:genetics* / Q000821:virology; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D010942:Plant Viruses / Q000472:pathogenicity*; D012328:RNA Viruses / Q000472:pathogenicity*; D060888:Real-Time Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics; D059467:Transcriptome
chemical_list: D014157:Transcription Factors