title: Flow cytometric analysis of Mixed phenotype acute leukemia: experience from a tertiary oncology center.
abstract: BACKGROUND
  Mixed phenotype acute leukemia (MPAL) is a rare subset of acute leukemia where the blasts exhibit lineage specific antigens of more than one lineage. Flow cytometric immunophenotyping is essential for the diagnosis of MPAL and the accurate diagnosis highly depends on the panel of markers used. The precise incidence of MPAL is uncertain as various institutions use different combinations of antibodies to assign the blasts to a particular lineage.
  
  
  OBJECTIVE
  The aim was to study the immunoprofile of acute leukemia including aberrant antigen expressions and to study the incidence, clinical features, laboratory findings, and immunophenotype of MPAL in our institution.
  
  
  METHODS
  All cases of acute leukemias in which flow cytometric analysis during 1-year period from July 2012 to July 2013 were included in this study.
  
  
  RESULTS
  During the study period, flow cytometric analysis of 506 cases was performed. B lymphoblastic leukemia was the most common subtype of acute leukemia. CD13 was the most common aberrant antigen expression in acute lymphoblastic leukemia and CD7 was the most common aberrant antigen expression in acute myeloid leukemia. A diagnosis of MPAL was made in 15 cases, which accounted for 2.96% of all leukemias. 9 cases were diagnosed as T/myeloid, 5 cases as B/myeloid and 1 case as B/T.
  
  
  CONCLUSIONS
  Mixed phenotype acute leukemia is a rare subset of acute leukemia. Flow cytometry is critical in establishing a diagnosis of MPAL. The panel of antibodies used is important in the identification of the "mixed" phenotype. Cytoplasmic markers (cytoplasmic MPO, cytoplasmic 79a, cytoplasmic 22 and cytoplasmic CD3) should be included in the primary flow cytometric panel.
authors.forename: Renu
authors.initials: R
authors.lastname: Sukumaran
authors.affiliation: Division of Pathology, Regional Cancer Centre, Trivandrum, Kerala, India.
authors.forename: Rekha A
authors.initials: RA
authors.lastname: Nair
authors.forename: Priya Mary
authors.initials: PM
authors.lastname: Jacob
authors.forename: Kunjulekshmi Amma Raveendran
authors.initials: KA
authors.lastname: Nair Anila
authors.forename: Shruthy
authors.initials: S
authors.lastname: Prem
authors.forename: Rajeswary
authors.initials: R
authors.lastname: Binitha
authors.forename: Parukuttyamma
authors.initials: P
authors.lastname: Kusumakumary
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D016130:Immunophenotyping / Q000379:methods*; D007223:Infant; D015456:Leukemia, Biphenotypic, Acute / Q000453:epidemiology* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D062606:Tertiary Care Centers; D055815:Young Adult