title: Cytogenetic significance of chromosome 17 aberrations and P53 gene mutations as prognostic markers in oral squamous cell carcinoma.
abstract: BACKGROUND
  Cytogenetic analysis has detected an accumulation of genetic lesions in oral cancers. Numerical changes in chromosome 17 might be associated with an up-regulation of p53 gene, and could contribute to critical events in carcinogenesis. The aim of this study was to reveal possible correlations between the numerical aberrations of chromosome 17, deletion or amplification of the P53 gene and histological grading in patients with oral squamous cell carcinoma (OSCC).
  
  
  METHODS
  This study was performed retrospectively on anonymous forty paraffin embedded specimens diagnosed with a primary OSCC. Sections were prepared for p53 immunohistochemical staining and FISH technique evaluation.
  
  
  RESULTS
  All studied cases showed a positive nuclear staining with different indices for the p53 protein. Furthermore, statistical analysis showed a significant difference between all histological types of OSCC. In term of P53 immunoreactivity well differentiated OSCC showed the highest, whereas poorly differentiated showed weakest. Regarding chromosome 17 aberrations and p53 gene mutations, Spearman correlation test revealed a statistical significant positive correlation between chromosome 17 abnormalities and p53 gene mutations as well as with the immunohistochemical expression of p53 proteins. Moreover, the positive association between p53 gene mutations and the expression of p53 protein was statistically significant.
  
  
  CONCLUSIONS
  In the light of the previous findings, we concluded that numerical aberrations of chromosome 17 and p53 gene mutations as well as expression of p53 protein have enormous influence on various cellular processes including differentiation and carcinogenesis. Such knowledge provides an easy and simplified approach to prognosis predilection for OSCC.
  The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/13000_2015_232 .
authors.affiliation: Department of Oral and maxillofacial Pathology, Faculty of Dentistry, Mansoura University, Mansoura, Egypt. zedanwalid@hotmail.com.
authors.forename: Walid
authors.initials: W
authors.lastname: Zedan
authors.affiliation: Department of Oral and maxillofacial Pathology, Faculty of Dentistry, Mansoura University, Mansoura, Egypt. drmourad11@yahoo.com.
authors.forename: Mohamed I
authors.initials: MI
authors.lastname: Mourad
authors.affiliation: Department of Clinical Pathology-Hematology, Faculty of Medicine, Mansoura University, Mansoura, Egypt. sherin_abdaziz@yahoo.com.
authors.forename: Sherin M Abd
authors.initials: SM
authors.lastname: El-Aziz
authors.affiliation: Department of Oral and maxillofacial Pathology, Faculty of Dentistry, Mansoura University, Mansoura, Egypt. naglasalama@hotmail.com.
authors.forename: Nagla M
authors.initials: NM
authors.lastname: Salamaa
authors.affiliation: Department of General Pathology, Faculty of Medicine, Mansoura University, Mansoura, Egypt. asemali444@yahoo.com.
authors.forename: Asem A
authors.initials: AA
authors.lastname: Shalaby
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000737:chemistry / Q000235:genetics* / Q000473:pathology; D002454:Cell Differentiation; D002869:Chromosome Aberrations; D002886:Chromosomes, Human, Pair 17; D004252:DNA Mutational Analysis / Q000379:methods; D005260:Female; D020022:Genetic Predisposition to Disease; D006258:Head and Neck Neoplasms / Q000737:chemistry / Q000235:genetics* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D009062:Mouth Neoplasms / Q000737:chemistry / Q000235:genetics* / Q000473:pathology; D009154:Mutation; D060787:Neoplasm Grading; D010641:Phenotype; D011237:Predictive Value of Tests; D012189:Retrospective Studies; D000077195:Squamous Cell Carcinoma of Head and Neck; D016159:Tumor Suppressor Protein p53 / Q000032:analysis / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53