title: Expression of nephronectin is inhibited by oncostatin M via both JAK/STAT and MAPK pathways.
abstract: Nephronectin (Npnt), also called POEM, is an extracellular matrix protein considered to play critical roles as an adhesion molecule in the development and functions of various tissues, such as the kidneys, liver, and bones. In the present study, we examined the molecular mechanism of Npnt gene expression and found that oncostatin M (OSM) strongly inhibited Npnt mRNA expression in MC3T3-E1 cells from a mouse osteoblastic cell line. OSM also induced a decrease in Npnt expression in both time- and dose-dependent manners via both the JAK/STAT and MAPK pathways. In addition, OSM-induced inhibition of osteoblast differentiation was recovered by over-expression of Npnt. These results suggest that OSM inhibits Npnt expression via the JAK/STAT and MAPK pathways, while down-regulation of Npnt by OSM influences inhibition of osteoblast differentiation.
authors.affiliation: Department of Biochemistry, School of Dentistry, Showa University, Shinagawa, Tokyo 142-8555, Japan ; Division of Biological Chemistry, Department of Molecular Biology, Showa University School of Pharmacy, Shinagawa, Tokyo 142-8555, Japan.
authors.forename: Tamaki
authors.initials: T
authors.lastname: Kurosawa
authors.affiliation: Department of Biochemistry, School of Dentistry, Showa University, Shinagawa, Tokyo 142-8555, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Yamada
authors.affiliation: Department of Pharmacology, School of Dentistry, Showa University, Ohta, Tokyo 145-8515, Japan.
authors.forename: Masamichi
authors.initials: M
authors.lastname: Takami
authors.affiliation: Department of Biochemistry, School of Dentistry, Showa University, Shinagawa, Tokyo 142-8555, Japan.
authors.forename: Dai
authors.initials: D
authors.lastname: Suzuki
authors.affiliation: Department of Biochemistry, School of Dentistry, Showa University, Shinagawa, Tokyo 142-8555, Japan ; Department of Oral and Maxillofacial Surgery, School of Dentistry, Showa University, Ohta, Tokyo 145-8515, Japan.
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Saito
authors.affiliation: Department of Biochemistry, School of Dentistry, Showa University, Shinagawa, Tokyo 142-8555, Japan ; Department of Perioperative Medicine Division of Anesthesiology, School of Dentistry, Showa University, Ohta, Tokyo 145-8515, Japan.
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Hiranuma
authors.affiliation: Department of Biochemistry, School of Dentistry, Showa University, Shinagawa, Tokyo 142-8555, Japan ; Department of Periodontology, School of Dentistry, Showa University, Ohta, Tokyo 145-8515, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Enomoto
authors.affiliation: Brain Science Laboratory, The Research Organization of Science and Technology, Ritsumeikan University, Kusatsu, Shiga 525-8577, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Morimura
authors.affiliation: Department of Periodontology, School of Dentistry, Showa University, Ohta, Tokyo 145-8515, Japan.
authors.forename: Matsuo
authors.initials: M
authors.lastname: Yamamoto
authors.affiliation: Department of Perioperative Medicine Division of Anesthesiology, School of Dentistry, Showa University, Ohta, Tokyo 145-8515, Japan.
authors.forename: Takehiko
authors.initials: T
authors.lastname: Iijima
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Dentistry, Showa University, Ohta, Tokyo 145-8515, Japan.
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Shirota
authors.affiliation: Division of Biological Chemistry, Department of Molecular Biology, Showa University School of Pharmacy, Shinagawa, Tokyo 142-8555, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Itabe
authors.affiliation: Department of Biochemistry, School of Dentistry, Showa University, Shinagawa, Tokyo 142-8555, Japan.
authors.forename: Ryutaro
authors.initials: R
authors.lastname: Kamijo
keywords: BMP-2, bone morphogenetic protein-2; ERK, extracellular signal-regulated kinase; JAK, janus kinase; JAK/STAT; JNK, c-Jun N-terminal kinase; MAM, meprin, A5 protein, and receptor protein-tyrosine phosphatase μ; MAPK; MAPK, mitogen-activated protein kinase; MEF2, myocyte enhancer-binding factor 2A; Nephronectin; Npnt, nephronectin; OSM, oncostatin M; OSMR, OSM receptor; Oncostatin M; STAT, signal transducer and activator of transcription; TGF-β, transforming growth factor-β; TNF-α, tumor necrosis factor-α