title: Inter-individual variability in the response of human peripheral blood lymphocytes to ionizing radiation: comparison of the dicentric and micronucleus assays.
abstract: Ionizing radiation can induce a wide range of DNA damage that leads to chromosomal aberrations. Some of those aberrations (dicentrics and micronuclei) are applied in biodosimetry. Biological dosimetry assumes similar radiosensitivity of each donor, but it does not exclude inter-individual variations in radiation susceptibility. Therefore, for biological reasons, it is always challenging to investigate inter-individual variability in response to radiation. For mechanistic reasons, it is also interesting to investigate the correlation between dicentric and micronuclei formation in response to radiation. In this experiment, irradiated blood specimens from 14 healthy male and female donors have been used to evaluate inter-individual variability in response to the genotoxic effects of X-ray radiation, as well as the dose-response relationship and test sensitivity using two endpoints (dicentrics and micronuclei). The results showed similar patterns of cytogenetic biomarker distribution between donors, but differences in the response of some donors at some doses. Data also showed that responses of male donors were better detected using the dicentric test, while for females, micronucleus frequencies were higher in response to the same dose of radiation. No influence of smoking status or age on specific responses was observed. Group variability in response to radiation was evaluated using coefficient of variation for each group of individuals irradiated with the same doses; as the dose increases, group variability becomes substantially lower. Despite sporadic inter-individual variability, trend of radiation-induced changes was similar. Produced calibration curves for both types of damage revealed dicentrics as genetic damage more typical for radiation than micronuclei.
authors.affiliation: Serbian Institute of Occupational Health "Dr Dragomir Karajovic", Deligradska 29, Belgrade, Serbia, jejapajic@gmail.com.
authors.forename: Jelena
authors.initials: J
authors.lastname: Pajic
authors.forename: Boban
authors.initials: B
authors.lastname: Rakic
authors.forename: Branislav
authors.initials: B
authors.lastname: Rovcanin
authors.forename: Dubravka
authors.initials: D
authors.lastname: Jovicic
authors.forename: Ivana
authors.initials: I
authors.lastname: Novakovic
authors.forename: Aleksandar
authors.initials: A
authors.lastname: Milovanovic
authors.forename: Vesna
authors.initials: V
authors.lastname: Pajic
mesh_terms: D000328:Adult; D002869:Chromosome Aberrations; D002877:Chromosomes, Human / Q000528:radiation effects; D020732:Cytogenetic Analysis / Q000379:methods; D004249:DNA Damage; D004307:Dose-Response Relationship, Radiation; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000528:radiation effects*; D008297:Male; D015162:Micronucleus Tests / Q000379:methods*; D008875:Middle Aged; D011836:Radiation Tolerance; D014965:X-Rays / Q000009:adverse effects