title: Phylogenetic Analysis of the Spike (S) Gene of the New Variants of Porcine Epidemic Diarrhoea Virus in Taiwan.
abstract: New variants of porcine epidemic diarrhoea virus (PEDV), which emerged in Taiwan in late 2013, have caused a high morbidity and mortality in neonatal piglets. To investigate the molecular characteristics of the spike (S) gene of the emerging Taiwan PEDV strains for a better understanding of the genetic diversity and relationship among the Taiwan new variants and the global PEDVs, full-length S genes of PEDVs from nine 1-7 day-old piglets from three pig farms in the central and southern Taiwan were sequenced and analysed. The result of phylogenetic analysis of the S gene showed that all the Taiwan PEDV strains were closely related to the non-S INDEL strains from US, Canada and China, suggesting a common ancestor for these strains. As compared with the historic PEDVs and CV777-based vaccine strains, the nine Taiwan PEDV variants shared almost the same genetic signatures as the global non-S INDEL strains, including a series of insertions, deletions and mutations in the amino terminal as well as identical mutations in the neutralizing epitopes of the S gene. The high similarity of the S protein among the Taiwan and the globally emerged non-S INDEL PEDV strains suggests that the Taiwan new variants may share similar pathogenesis and immunogenicity as the global outbreak variants. The development of a novel vaccine based on the Taiwan or the global non-S INDEL strains may be contributive to the control of the current global porcine epidemic diarrhoea outbreaks.
authors.affiliation: Graduate Institute of Molecular and Comparative Pathobiology, School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan.
authors.forename: H-Y
authors.initials: HY
authors.lastname: Chiou
authors.affiliation: Animal Health Research Institute, Council of Agriculture, Tamsui, New Taipei City, Taiwan.
authors.forename: Y-L
authors.initials: YL
authors.lastname: Huang
authors.affiliation: Animal Health Research Institute, Council of Agriculture, Tamsui, New Taipei City, Taiwan.
authors.forename: M-C
authors.initials: MC
authors.lastname: Deng
authors.affiliation: Animal Health Research Institute, Council of Agriculture, Tamsui, New Taipei City, Taiwan.
authors.forename: C-Y
authors.initials: CY
authors.lastname: Chang
authors.affiliation: Graduate Institute of Molecular and Comparative Pathobiology, School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan.
authors.forename: C-R
authors.initials: CR
authors.lastname: Jeng
authors.affiliation: Graduate Institute of Molecular and Comparative Pathobiology, School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan.
authors.forename: P-S
authors.initials: PS
authors.lastname: Tsai
authors.affiliation: Graduate Institute of Molecular and Comparative Pathobiology, School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan.
authors.forename: C
authors.initials: C
authors.lastname: Yang
authors.affiliation: Graduate Institute of Molecular and Comparative Pathobiology, School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan.
authors.forename: V F
authors.initials: VF
authors.lastname: Pang
authors.affiliation: Graduate Institute of Molecular and Comparative Pathobiology, School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan.
authors.forename: H-W
authors.initials: HW
authors.lastname: Chang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018352:Coronavirus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny; D053485:Porcine epidemic diarrhea virus / Q000235:genetics / Q000502:physiology*; D016415:Sequence Alignment / Q000662:veterinary; D064370:Spike Glycoprotein, Coronavirus / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000821:virology; D013624:Taiwan / Q000453:epidemiology
chemical_list: D064370:Spike Glycoprotein, Coronavirus
keywords: molecular characterization; phylogenetic analysis; porcine epidemic diarrhoea virus; spike glycoprotein gene