title: Red-shifted red/green-type cyanobacteriochrome AM1_1870g3 from the chlorophyll d-bearing cyanobacterium Acaryochloris marina.
abstract: Cyanobacteriochromes (CBCRs) are diverse photoreceptors that are found only from cyanobacteria and cover wide range of light qualities. CBCRs are divided into two types regarding the chromophore species they contain: phycocyanobilin (PCB) and phycoviolobilin. Red/green-type CBCRs are widely distributed subfamily among the PCB-binding CBCRs and photoconvert between a red-absorbing thermostable form and a green-absorbing metastable form. Our recent study discovered that a red/green-type CBCR, AM1_1557g2, from a cyanobacterium Acaryochloris marina covalently binds not only PCB but also biliverdin (BV). BV-binding AM1_1557g2 photoconverts between a far-red absorbing form and an orange-absorbing form. We report, herein, that another red/green-type CBCR, AM1_1870g3, from the cyanobacterium A. marina also bound both PCB and BV. PCB- and BV-binding ones showed red/green and far-red/orange reversible photoconversions, respectively. Unexpectedly, absorbing wavelengths are 10-20 nm red-shifted compared with those of AM1_1557g2. These red-shifted characteristics may be useful for optogenetic light switches that work in various organisms.
authors.affiliation: Department of Biological Science, Faculty of Science, Shizuoka University, Ohya, Suruga-ku, Shizuoka 422-8529, Japan; Precursory Research for Embryonic Science and Technology, Japan Science and Technology Agency, 4-1-8 Honcho Kawaguchi, Saitama 332-0012 Japan. Electronic address: narikawa.rei@shizuoka.ac.jp.
authors.forename: Rei
authors.initials: R
authors.lastname: Narikawa
authors.affiliation: Department of Biological Science, Faculty of Science, Shizuoka University, Ohya, Suruga-ku, Shizuoka 422-8529, Japan.
authors.forename: Keiji
authors.initials: K
authors.lastname: Fushimi
authors.affiliation: Department of Life Sciences (Biology), Graduate School of Art and Sciences, University of Tokyo, Komaba, Meguro, Tokyo 153-8902, Japan.
authors.lastname: Ni-Ni-Win
authors.affiliation: Department of Life Sciences (Biology), Graduate School of Art and Sciences, University of Tokyo, Komaba, Meguro, Tokyo 153-8902, Japan; Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, 4-1-8 Honcho Kawaguchi, Saitama 332-0012, Japan.
authors.forename: Masahiko
authors.initials: M
authors.lastname: Ikeuchi
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D001664:Biliverdine / Q000737:chemistry / Q000378:metabolism; D000458:Cyanobacteria / Q000737:chemistry / Q000378:metabolism*; D008027:Light; D055668:Photochemical Processes; D052980:Phycobilins / Q000737:chemistry / Q000378:metabolism; D010798:Phycocyanin / Q000737:chemistry / Q000378:metabolism; D010860:Pigments, Biological / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D013056:Spectrophotometry, Ultraviolet
chemical_list: D001426:Bacterial Proteins; D052980:Phycobilins; D010860:Pigments, Biological; D010798:Phycocyanin; C011069:phycocyanobilin; D001664:Biliverdine
keywords: Bilin; Biliverdin; Cyanobacteria; Cyanobacteriochrome; Photoreceptor