title: Application of GC-MS coupled with chemometrics for scanning serum metabolic biomarkers from renal fibrosis rat.
abstract: Renal interstitial fibrosis closely relates to chronic kidney disease and is regarded as the final common pathway in most cases of end-stage renal disease. Metabolomic biomarkers can facilitate early diagnosis and allow better understanding of the pathogenesis underlying renal fibrosis. Gas chromatography-mass spectrometry (GC/MS) is one of the most promising techniques for identification of metabolites. However, the existence of the background, baseline offset, and overlapping peaks makes accurate identification of the metabolites unachievable. In this study, GC/MS coupled with chemometric methods was successfully developed to accurately identify and seek metabolic biomarkers for rats with renal fibrosis. By using these methods, seventy-six metabolites from rat serum were accurately identified and five metabolites (i.e., urea, ornithine, citric acid, galactose, and cholesterol) may be useful as potential biomarkers for renal fibrosis.
authors.affiliation: Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China. Electronic address: liushao999@hotmail.com.
authors.forename: Shao
authors.initials: S
authors.lastname: Liu
authors.affiliation: Xiangya's Third Affiliated Hospital, Central South University, Changsha, Hunan 410008, PR China.
authors.forename: Ji-Shi
authors.initials: JS
authors.lastname: Liu
authors.affiliation: Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China.
authors.forename: Ren-Na
authors.initials: RN
authors.lastname: Luo
authors.affiliation: Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xu
authors.affiliation: Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China.
authors.forename: Wei-Ru
authors.initials: WR
authors.lastname: Zhang
authors.affiliation: Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Meng
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan 410008, PR China.
authors.forename: Yi-Zeng
authors.initials: YZ
authors.lastname: Liang
authors.affiliation: Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China. Electronic address: taolj@mail.csu.edu.cn.
authors.forename: Li-Jian
authors.initials: LJ
authors.lastname: Tao
mesh_terms: D000465:Algorithms; D000818:Animals; D015415:Biomarkers / Q000097:blood*; D001774:Blood Chemical Analysis / Q000379:methods*; D003627:Data Interpretation, Statistical; D005355:Fibrosis / Q000097:blood; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007668:Kidney / Q000378:metabolism*; D008297:Male; D015999:Multivariate Analysis; D051381:Rats; D017208:Rats, Wistar; D051436:Renal Insufficiency, Chronic / Q000097:blood* / Q000175:diagnosis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D015415:Biomarkers
keywords: Biomarker; Chemometrics; Gas chromatography–mass spectrometry; Metabolites; Renal fibrosis