title: Fgf signaling controls the telencephalic distribution of Fgf-expressing progenitors generated in the rostral patterning center.
abstract: BACKGROUND
  The rostral patterning center (RPC) secretes multiple fibroblast growth factors (Fgfs) essential for telencephalon growth and patterning. Fgf expression patterns suggest that they mark functionally distinct RPC subdomains. We generated Fgf8(CreER) and Fgf17(CreER) mice and used them to analyze the lineages of Fgf8- versus Fgf17-expressing RPC cells.
  
  
  RESULTS
  Both lineages contributed to medial structures of the rostroventral telencephalon structures including the septum and medial prefrontral cortex. In addition, RPC-derived progenitors were observed in other regions of the early telencephalic neuroepithelium and generated neurons in the olfactory bulb, neocortex, and basal ganglia. Surprisingly, Fgf8(+) RPC progenitors generated the majority of basal ganglia cholinergic neurons. Compared to the Fgf8 lineage, the Fgf17 lineage was more restricted in its early dispersion and its contributions to the telencephalon. Mutant studies suggested that Fgf8 and Fgf17 restrict spread of RPC progenitor subpopulations.
  
  
  CONCLUSIONS
  We identified the RPC as an important source of progenitors that contribute broadly to the telencephalon and found that two molecularly distinct progenitor subtypes in the RPC make different contributions to the developing forebrain.
authors.affiliation: Department of Psychiatry, University of California, 1550 4th Street, UCSF MC 2611, San Francisco, CA, 94158, USA. rhoch@plos.org.|Current address: PLOS, 1160 Battery Street, San Francisco, CA, 94111, USA. rhoch@plos.org.
authors.forename: Renée V
authors.initials: RV
authors.lastname: Hoch
authors.affiliation: Department of Psychiatry, University of California, 1550 4th Street, UCSF MC 2611, San Francisco, CA, 94158, USA. jefclarke@gmail.com.
authors.forename: Jeffrey A
authors.initials: JA
authors.lastname: Clarke
authors.affiliation: Department of Psychiatry, University of California, 1550 4th Street, UCSF MC 2611, San Francisco, CA, 94158, USA. john.rubenstein@ucsf.edu.
authors.forename: John L R
authors.initials: JL
authors.lastname: Rubenstein
mesh_terms: D000818:Animals; D001479:Basal Ganglia / Q000166:cytology / Q000196:embryology; D019521:Body Patterning / Q000502:physiology*; D019070:Cell Lineage; D059329:Cholinergic Neurons / Q000166:cytology; D051524:Fibroblast Growth Factor 8 / Q000235:genetics / Q000502:physiology*; D005346:Fibroblast Growth Factors / Q000235:genetics / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D055879:Gene Knock-In Techniques; D005813:Genes, Synthetic; D005865:Gestational Age; D051379:Mice; D058953:Neural Stem Cells / Q000145:classification / Q000166:cytology*; D009830:Olfactory Bulb / Q000166:cytology / Q000196:embryology; D016548:Prosencephalon / Q000166:cytology / Q000196:embryology; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D015398:Signal Transduction / Q000502:physiology; D013687:Telencephalon / Q000166:cytology* / Q000196:embryology
chemical_list: C484726:Fgf17 protein, mouse; C496457:Fgf8 protein, mouse; D011993:Recombinant Fusion Proteins; D051524:Fibroblast Growth Factor 8; D005346:Fibroblast Growth Factors