title: Hypoxia inhibits semicarbazide-sensitive amine oxidase activity in adipocytes.
abstract: Semicarbazide-sensitive amine oxidase (SSAO), an enzyme highly expressed on adipocyte plasma membranes, converts primary amines into aldehydes, ammonium and hydrogen peroxide, and is likely involved in endothelial damage during the course of diabetes and obesity. We investigated whether in vitro, adipocyte SSAO was modulated under hypoxic conditions that is present in adipose tissue from obese or intensive care unit. Physical or pharmacological hypoxia decreased SSAO activity in murine adipocytes and human adipose tissue explants, while enzyme expression was preserved. This effect was time-, dose-dependent and reversible. This down-regulation was confirmed in vivo in subcutaneous adipose tissue from a rat model of hypoxia. Hypoxia-induced suppression in SSAO activity was independent of the HIF-1-α pathway or of oxidative stress, but was partially antagonized by medium acidification. Hypoxia-induced down-regulation of SSAO activity could represent an adaptive mechanism to lower toxic molecules production, and may thus protect from tissue injury during these harmful conditions.
authors.affiliation: UMR S_1185, INSERM, Université Paris-Sud, Le Kremlin-Bicêtre, France; Service de Réanimation Médico-Chirurgicale, pôle Thorax-Vaisseaux-Abdomen-Métabolisme, Hôpital Ambroise Paré, Assistance Publique-Hôpitaux de Paris, Boulogne-Billancourt, France. Electronic address: xavier.repesse@apr.aphp.fr.
authors.forename: Xavier
authors.initials: X
authors.lastname: Repessé
authors.affiliation: Centre de Recherche Saint-Antoine, INSERM, UMR S_938, Sorbonne Universités, Université Paris 6, Paris, France; Institut Hospitalo-Universitaire ICAN, Paris, France.
authors.forename: Marthe
authors.initials: M
authors.lastname: Moldes
authors.affiliation: UMR S_1185, INSERM, Université Paris-Sud, Le Kremlin-Bicêtre, France; Centre de Recherche Saint-Antoine, INSERM, UMR S_938, Sorbonne Universités, Université Paris 6, Paris, France; Institut Hospitalo-Universitaire ICAN, Paris, France.
authors.forename: Adeline
authors.initials: A
authors.lastname: Muscat
authors.affiliation: Centre de Recherche Saint-Antoine, INSERM, UMR S_938, Sorbonne Universités, Université Paris 6, Paris, France; Institut Hospitalo-Universitaire ICAN, Paris, France; Service d'Endocrinologie, Hôpital Saint-Antoine, Assistance Publique-Hôpitaux de Paris, Paris, France.
authors.forename: Camille
authors.initials: C
authors.lastname: Vatier
authors.affiliation: UMR S_1185, INSERM, Université Paris-Sud, Le Kremlin-Bicêtre, France; Centre de Recherche Saint-Antoine, INSERM, UMR S_938, Sorbonne Universités, Université Paris 6, Paris, France; Institut Hospitalo-Universitaire ICAN, Paris, France; Service d'Endocrinologie, Hôpital Saint-Antoine, Assistance Publique-Hôpitaux de Paris, Paris, France.
authors.forename: Gérard
authors.initials: G
authors.lastname: Chetrite
authors.affiliation: Université Paris 13, Sorbonne Paris Cité, EA2363 Bobigny, France; Service d'Explorations Fonctionnelles, Hôpital Avicenne, Assistance Publique-Hôpitaux de Paris, Bobigny, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Gille
authors.affiliation: Université Paris 13, Sorbonne Paris Cité, EA2363 Bobigny, France; Service d'Explorations Fonctionnelles, Hôpital Avicenne, Assistance Publique-Hôpitaux de Paris, Bobigny, France.
authors.forename: Carole
authors.initials: C
authors.lastname: Planes
authors.affiliation: INSERM U1116, Faculté de Médecine, Vandoeuvre-les-Nancy, France.
authors.forename: Anna
authors.initials: A
authors.lastname: Filip
authors.affiliation: INSERM U1116, Faculté de Médecine, Vandoeuvre-les-Nancy, France; Université de Lorraine, Nancy, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Mercier
authors.affiliation: Service d'Anesthésie-Réanimation, Hôpital Bicêtre, Assistance Publique-Hôpitaux de Paris, Le Kremlin-Bicêtre, France; Microcirculation, Bioénergétique, Inflammation et Insuffisance Circulatoire Aigue, Equipe Universitaire 3509, Paris VII-Paris XI-Paris XIII, Paris, France.
authors.forename: Jacques
authors.initials: J
authors.lastname: Duranteau
authors.affiliation: UMR S_1185, INSERM, Université Paris-Sud, Le Kremlin-Bicêtre, France; Centre de Recherche Saint-Antoine, INSERM, UMR S_938, Sorbonne Universités, Université Paris 6, Paris, France; Institut Hospitalo-Universitaire ICAN, Paris, France; Service d'Endocrinologie, Hôpital Saint-Antoine, Assistance Publique-Hôpitaux de Paris, Paris, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Fève
mesh_terms: D016475:3T3 Cells; D017667:Adipocytes / Q000378:metabolism*; D006631:Amine Oxidase (Copper-Containing) / Q000378:metabolism*; D000818:Animals; D015536:Down-Regulation; D006801:Humans; D000860:Hypoxia / Q000378:metabolism*; D051379:Mice; D018384:Oxidative Stress / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D006631:Amine Oxidase (Copper-Containing)
keywords: Adipocyte; Diabetes; Hypoxia; Obesity; SSAO