title: Rapid Detection of Acquired and Inducible Clarithromycin Resistance in Mycobacterium abscessus Group by a Simple Real-Time PCR Assay.
abstract: By targeting the erm(41) and rrl genes in the Mycobacterium abscessus group, a multiplex real-time PCR assay for clarithromycin resistance showed 95% (38/40) concordance with nucleic acid testing and 95% (37/39) concordance with phenotypic testing. This assay provides a simple and rapid alternative to extended incubation or erm(41) sequencing.
authors.affiliation: Department of Pathology, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Robert F
authors.initials: RF
authors.lastname: Luo
authors.affiliation: Clinical Microbiology Laboratory, Stanford Health Care, Palo Alto, California, USA.
authors.forename: Cheyenne
authors.initials: C
authors.lastname: Curry
authors.affiliation: Clinical Microbiology Laboratory, Stanford Health Care, Palo Alto, California, USA.
authors.forename: Nathan
authors.initials: N
authors.lastname: Taylor
authors.affiliation: Clinical Microbiology Laboratory, Stanford Health Care, Palo Alto, California, USA.
authors.forename: Indre
authors.initials: I
authors.lastname: Budvytiene
authors.affiliation: Department of Pathology, Stanford University School of Medicine, Stanford, California, USA Clinical Microbiology Laboratory, Stanford Health Care, Palo Alto, California, USA Department of Medicine, Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, California, USA nbanaei@stanford.edu.
authors.forename: Niaz
authors.initials: N
authors.lastname: Banaei
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017291:Clarithromycin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005838:Genotype; D006801:Humans; D008826:Microbial Sensitivity Tests; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D009165:Mycobacterium Infections, Nontuberculous / Q000382:microbiology; D009170:Nontuberculous Mycobacteria / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D010641:Phenotype; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D013781:Texas; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D017291:Clarithromycin