title: Development of a Rapid Immunochromatographic Lateral Flow Device Capable of Differentiating Phytase Expressed from Recombinant Aspergillus niger phyA2 and Genetically Modified Corn.
abstract: Phytase is a phosphohydrolase considered highly specific for the degradation of phytate to release bound phosphorus for animal consumption and aid in the reduction of environmental nutrient loading. New sources of phytase have been sought that are economically and efficiently productive including the construction of genetically modified (GM) phytase products designed to bypass the costs associated with feed processing. Four monoclonal antibodies (EH10a, FA7, AF9a, and CC1) raised against recombinant Aspergillus niger phyA2 were used to develop a highly specific and sensitive immunochromatographic lateral flow device for rapid detection of transgenic phytase, such as in GM corn. Antibodies sequentially paired and tested along lateral flow strips showed that the EH10a-FA7 antibody pair was able to detect the recombinant yeast-phytase at 5 ng/mL, whereas the AF9a-CC1 antibody pair to GM phytase corn was able to detect at 2 ng/mL. Concurrent to this development, evidence was revealed which suggests that antibody binding sites may be glycosylated.
authors.affiliation: †Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 12 Zhongguancun Nandajie, Beijing 100081, People's Republic of China.
authors.forename: Xiaojin
authors.initials: X
authors.lastname: Zhou
authors.affiliation: ‡Artron BioResearch Inc., 3938 North Fraser Way, Burnaby, British Columbia V5J 5H6, Canada.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Hui
authors.forename: Xiao-Lin
authors.initials: XL
authors.lastname: Yu
authors.forename: Zhen
authors.initials: Z
authors.lastname: Lin
authors.affiliation: †Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 12 Zhongguancun Nandajie, Beijing 100081, People's Republic of China.
authors.forename: Ling-Kui
authors.initials: LK
authors.lastname: Pu
authors.forename: Zhiguan
authors.initials: Z
authors.lastname: Tu
authors.affiliation: ‡Artron BioResearch Inc., 3938 North Fraser Way, Burnaby, British Columbia V5J 5H6, Canada.|#Ji Nan Kangbo Biotechnology, 2711 Ying Xiu Road, Jinan, Shandong Province 250101, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: #Ji Nan Kangbo Biotechnology, 2711 Ying Xiu Road, Jinan, Shandong Province 250101, People's Republic of China.|⊥Beijing Artron Jingbiao Biotech Inc., 19 Tianrong Street, Daxing Bio-medicine Industry Park, Daxing District, Beijing 102600, People's Republic of China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
authors.forename: Jian
authors.initials: J
authors.lastname: Zheng
authors.affiliation: ΔBlood Transfusion Department, Second Affiliated Hospital of Chongqing Medical University, 1 Yixueyuan Road, Yuzhong District, Chongqing 400016, People's Republic of China.
authors.forename: Juan
authors.initials: J
authors.lastname: Zhang
mesh_terms: D010832:6-Phytase / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D001234:Aspergillus niger / Q000737:chemistry / Q000201:enzymology*; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D005656:Fungal Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D010833:Phytic Acid / Q000378:metabolism; D010940:Plant Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D003313:Zea mays / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics
chemical_list: D005656:Fungal Proteins; D010940:Plant Proteins; D010833:Phytic Acid; D010832:6-Phytase
keywords: GM corn; glycosylation; lateral strips; monoclonal antibodies; phytase