title: Electrical properties of isolated cardiomyocytes in a rat model of thiamine deficiency.
abstract: In modern society, thiamine deficiency (TD) remains an important medical condition linked to altered cardiac function. There have been contradictory reports about the impact of TD on heart physiology, especially in the context of cardiac excitability. In order to address this particular question, we used a TD rat model and patch-clamp technique to investigate the electrical properties of isolated cardiomyocytes from epicardium and endocardium. Neither cell type showed substantial differences on the action potential waveform and transient outward potassium current. Based on our results we can conclude that TD does not induce major electrical remodeling in isolated cardiac myocytes in either endocardium or epicardium cells.
authors.affiliation: Universidade Federal de Minas Gerais, Minas Gerais, MG, Brazil.
authors.forename: Artur
authors.initials: A
authors.lastname: Santos-Miranda
authors.affiliation: Universidade Federal de Minas Gerais, Minas Gerais, MG, Brazil.
authors.forename: Jader Santos
authors.initials: JS
authors.lastname: Cruz
authors.affiliation: Universidade Federal de Minas Gerais, Minas Gerais, MG, Brazil.
authors.forename: Danilo
authors.initials: D
authors.lastname: Roman-Campos
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000818:Animals; D004195:Disease Models, Animal; D022062:Electrophysiologic Techniques, Cardiac; D004699:Endocardium / Q000166:cytology; D008297:Male; D032383:Myocytes, Cardiac / Q000502:physiology*; D018408:Patch-Clamp Techniques; D010496:Pericardium / Q000166:cytology; D017208:Rats, Wistar; D013832:Thiamine Deficiency / Q000503:physiopathology*; D013997:Time Factors