title: Dual-responsive polymer coated superparamagnetic nanoparticle for targeted drug delivery and hyperthermia treatment.
abstract: In this work, we have prepared water-soluble superparamgnetic iron oxide nanoparticles (SPIONs) coated with a dual responsive polymer for targeted delivery of anticancer hydrophobic drug (curcumin) and hyperthermia treatment. Herein, superparamagnetic mixed spinel (MnFe2O4) was used as a core material (15-20 nm) and modified with carboxymethyl cellulose (water-soluble component), folic acid (tagging agent), and dual responsive polymer (poly-N isopropylacrylamide-co-poly glutamic acid) by microwave radiation. Lower critical solution temperature (LCST) of the thermoresponsive copolymer was observed to be around 40 °C, which is appropriate for drug delivery. The polymer-SPIONs show high drug loading capacity (89%) with efficient and fast drug release at the desired pH (5.5) and temperature (40 °C) conditions. Along with this, the SPIONs show a very fast increase in temperature (45 °C in 2 min) when interacting with an external magnetic field, which is an effective and appropriate temperature for the localized hyperthermia treatment of cancer cells. The cytocompatibility of the curcumin loaded SPIONs was studied by the methyl thiazol tetrazolium bromide (MTT) assay, and cells were imaged by fluorescence microscopy. To explore the targeting behavior of curcumin loaded SPIONs, a simple magnetic capturing system (simulating a blood vessel) was constructed and it was found that ∼99% of the nanoparticle accumulated around the magnet in 2 min by traveling a distance of 30 cm. Along with this, to explore an entirely different aspect of the responsive polymer, its antibacterial activity toward an E. coli strain was also studied. It was found that responsive polymer is not harmful for normal or cancer cells but shows a good antibacterial property.
authors.affiliation: †Department of Applied Chemistry, Indian School of Mines, Dhanbad, Jharkhand 826 004, India.
authors.forename: Santanu
authors.initials: S
authors.lastname: Patra
authors.affiliation: †Department of Applied Chemistry, Indian School of Mines, Dhanbad, Jharkhand 826 004, India.
authors.forename: Ekta
authors.initials: E
authors.lastname: Roy
authors.affiliation: †Department of Applied Chemistry, Indian School of Mines, Dhanbad, Jharkhand 826 004, India.
authors.forename: Paramita
authors.initials: P
authors.lastname: Karfa
authors.affiliation: †Department of Applied Chemistry, Indian School of Mines, Dhanbad, Jharkhand 826 004, India.
authors.forename: Sunil
authors.initials: S
authors.lastname: Kumar
authors.affiliation: †Department of Applied Chemistry, Indian School of Mines, Dhanbad, Jharkhand 826 004, India.
authors.forename: Rashmi
authors.initials: R
authors.lastname: Madhuri
authors.affiliation: ‡Functional Nanomaterials Research Laboratory, Department of Applied Physics, Indian School of Mines, Dhanbad, Jharkhand 826 004, India.
authors.forename: Prashant K
authors.initials: PK
authors.lastname: Sharma
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D020099:Coated Materials, Biocompatible / Q000008:administration & dosage / Q000138:chemical synthesis; D003131:Combined Modality Therapy / Q000379:methods; D003474:Curcumin / Q000008:administration & dosage* / Q000737:chemistry; D003911:Dextrans / Q000008:administration & dosage* / Q000737:chemistry*; D004058:Diffusion; D006801:Humans; D006979:Hyperthermia, Induced / Q000379:methods*; D061986:MCF-7 Cells; D060526:Magnetic Fields; D058185:Magnetite Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry*; D053769:Nanocapsules / Q000008:administration & dosage / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011108:Polymers / Q000737:chemistry* / Q000528:radiation effects
chemical_list: D000970:Antineoplastic Agents; D020099:Coated Materials, Biocompatible; D003911:Dextrans; D058185:Magnetite Nanoparticles; D053769:Nanocapsules; D011108:Polymers; C097921:ferumoxtran-10; D003474:Curcumin
keywords: cell imaging; curcumin; cytotoxicity study; dual responsive polymer; hyperthermia; superparamagnetic nanoparticle; targeted drug delivery