title: A single dose biodegradable vaccine depot that induces persistently high levels of antibody over a year.
abstract: In this study, we describe a biodegradable vaccine depot which persists in vivo for at least 4-months, provides synergistic adjuvant effects and also allows dose sparing of both antigen and adjuvant. A single administration results in immediate release of a priming dose of vaccine, by a process of syneresis, which is then followed by release of remaining vaccine which maintains robust antibody levels that last for more than a year. The platform technology comprises two aqueous components; one contains chitosan and hydroxyapatite, in which the vaccine is incorporated, and the other consists of a crosslinking agent, tripolyphosphate (TPP) and chondroitin sulphate. When co-injected into tissue, they spontaneously crosslink forming a firm yet compliant vaccine-containing depot. Whole body imaging of animals inoculated with the material show that the depot persists in situ for up to 19 weeks. Vaccination of mice with depot formulations containing ovalbumin (OVA) emulsified in Montanide ISA 61 adjuvant results in the induction of robust antibody responses using doses of adjuvant 40-fold less than those recommended by the manufacturer. Dose sparing effects were also apparent with antigen when delivered in the depot. Similar dose sparing effects were observed with Montanide ISA 50, complete and incomplete Freund's adjuvants but not with aluminium hydroxide nor Quil A. Antibody titres, induced by a single dose of antigen/adjuvant formulation incorporated in the depot, persisted at high levels for at least 55 weeks following a single dose of vaccine.
authors.affiliation: Department of Microbiology and Immunology, Peter Doherty Institute for Infection & Immunity, The University of Melbourne, Parkville 3010, Australia. Electronic address: bychua@unimelb.edu.au.
authors.forename: Brendon Y
authors.initials: BY
authors.lastname: Chua
authors.affiliation: Department of Microbiology and Immunology, Peter Doherty Institute for Infection & Immunity, The University of Melbourne, Parkville 3010, Australia.
authors.forename: Toshiki
authors.initials: T
authors.lastname: Sekiya
authors.affiliation: Faculty of Science, Swinburne University of Technology, Hawthorn 3122, Australia.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Al Kobaisi
authors.affiliation: Department of Microbiology and Immunology, Peter Doherty Institute for Infection & Immunity, The University of Melbourne, Parkville 3010, Australia.
authors.forename: Kirsty R
authors.initials: KR
authors.lastname: Short
authors.affiliation: Faculty of Science, Swinburne University of Technology, Hawthorn 3122, Australia.
authors.forename: David E
authors.initials: DE
authors.lastname: Mainwaring
authors.affiliation: Department of Microbiology and Immunology, Peter Doherty Institute for Infection & Immunity, The University of Melbourne, Parkville 3010, Australia.
authors.forename: David C
authors.initials: DC
authors.lastname: Jackson
mesh_terms: D000818:Animals; D000917:Antibody Formation; D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D014612:Vaccines / Q000008:administration & dosage* / Q000276:immunology
chemical_list: D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D014612:Vaccines
keywords: Antibody; Chitosan; Depot; Hydroxyapatite; Single dose; Vaccine