title: Localization of the dominant non-enzymatic intermolecular cross-linking sites on fibrous collagen.
abstract: Previous studies have shown that fibrous collagen undergoes intermolecular cross-linking at multiple sites of the elongated triple-helical regions among adjacent juxtaposed collagen molecules on incubation with a very high concentration of reducing sugar such as 200 mM ribose, and the similarity of the changes in its physicochemical properties to that of senescent collagen aged in vivo has been emphasized. In the present study, however, it was found that when incubated with less than 30 mM ribose, fibrous collagen underwent intermolecular cross-linking primarily between the telopeptide region of a collagen molecule and the triple-helical region of another adjacent collagen molecule, and intermolecular cross-linking between the triple-helical regions of adjacent collagen molecules was very small. Physiological significance of the previous studies thus needs to be reevaluated.
authors.affiliation: Department of Biophysics, Kobe University Graduate School of Health Sciences, Suma, Kobe 654-0142, Japan; Laboratory of Food Chemistry, Faculty of Nutrition, Kobe Gakuin University, Nishi, Kobe 651-2180, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Chiue
authors.affiliation: Department of Biophysics, Kobe University Graduate School of Health Sciences, Suma, Kobe 654-0142, Japan.
authors.forename: Tsutako
authors.initials: T
authors.lastname: Yamazoye
authors.affiliation: Department of Biophysics, Kobe University Graduate School of Health Sciences, Suma, Kobe 654-0142, Japan; Department of Nutrition Management, Faculty of Health Sciences, Hyogo University, Hiraoka, Kakogawa 675-0195, Japan. Electronic address: matsumur@hyogo-dai.ac.jp.
authors.forename: Sueo
authors.initials: S
authors.lastname: Matsumura
mesh_terms: D000818:Animals; D003094:Collagen / Q000737:chemistry / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D010434:Pepsin A / Q000378:metabolism*; D051381:Rats; D012266:Ribose / Q000378:metabolism
chemical_list: D012266:Ribose; D003094:Collagen; D010434:Pepsin A
keywords: Collagen; Non-enzymatic cross-linking site; Rat tail tendon; Telopeptides