title: Resveratrol-Dependent Down-regulation of Receptor for Advanced Glycation End-products and Oxidative Stress in Kidney of Rats With Diabetes.
abstract: BACKGROUND
  Millions of people in the world have diabetes mellitus and its prevalence is growing. Oxidative stress, advanced glycation end-products (AGEs) and receptor for advanced glycation end-products (RAGE) play key role in the pathogenesis of diabetes. New and safe strategies of remedy are needed for this disease.
  
  
  OBJECTIVE
  We hypothesized that resveratrol may exert a renal protective effect on diabetic rats.
  
  
  METHODS
  Male rats with diabetes were treated with or without resveratrol as 1, 5, 10 mg/kg of body weight for 30 days. The total AGEs and malondialdehyde levels in kidney tissues were determined by spectroﬂuorimetric method and the insulin level was assayed using ELISA. The total antioxidant capacity contents in kidney and the glucose in plasma were measured by a colorimetric assay. The expression of RAGE was assayed in kidneys of all animals using quantitative PCR.
  
  
  RESULTS
  In resveratrol-treated rats with diabetes, malondialdehyde levels, plasma glucose and expression of RAGE were significantly reduced compared with the untreated group. Moreover, the total antioxidant and insulin levels significantly increased in treated rats. There was no significant difference in the AGEs contents among all the groups.
  
  
  CONCLUSIONS
  These results revealed that resveratrol has beneficial effects on kidney by extenuating the oxidative stress and down-regulation of RAGE expression.
authors.affiliation: Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran.
authors.forename: Heresh
authors.initials: H
authors.lastname: Moridi
authors.affiliation: Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran.
authors.forename: Jamshid
authors.initials: J
authors.lastname: Karimi
authors.affiliation: Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran.
authors.forename: Nasrin
authors.initials: N
authors.lastname: Sheikh
authors.affiliation: Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran.
authors.forename: Mohammad Taghi
authors.initials: MT
authors.lastname: Goodarzi
authors.affiliation: Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran.
authors.forename: Massoud
authors.initials: M
authors.lastname: Saidijam
authors.affiliation: Department of Molecular Medicine and Genetics, School of Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Yadegarazari
authors.affiliation: Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Khazaei
authors.affiliation: Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran.
authors.forename: Iraj
authors.initials: I
authors.lastname: Khodadadi
authors.affiliation: Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran.
authors.forename: Heidar
authors.initials: H
authors.lastname: Tavilani
authors.affiliation: Department of Biochemistry and Genetics, School of Medicine, Qazvin University of Medical Sciences, Qazvin, IR Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Piri
authors.affiliation: Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran.
authors.forename: Soheila
authors.initials: S
authors.lastname: Asadi
authors.affiliation: Department of Biochemistry, School of Medicine, Yazd University of Medical Sciences, Yazd, IR Iran.
authors.forename: Sadegh
authors.initials: S
authors.lastname: Zarei
authors.affiliation: Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran.
authors.forename: Azam
authors.initials: A
authors.lastname: Rezaei
keywords: Advanced Glycation End-product; Diabetes Mellitus; Oxidative Stress; Resveratrol