title: Knockdown of Adhesion-Regulating Molecule 1 Inhibits Proliferation in HL60 Cells.
abstract: OBJECTIVE
  Adhesion-regulating molecule 1 (ADRM1), a receptor located on the 26S proteasome, is upregulated in many solid cancers. However, little is known about its role in acute leukemia (AL).
  
  
  METHODS
  We determined ADRM1 expression levels in both untreated AL samples and leukemia cell lines using real-time polymerase chain reaction or Western blot analysis. Growth curves, colony formation assays, cell cycle and apoptosis analyses, cell migration and invasion assays and NF-κB p65 nuclear translocation assays via Western blotting were used to examine the biological behavior of HL60 cells and the underlying mechanism.
  
  
  RESULTS
  ADRM1 was upregulated in both untreated AL samples and leukemia cell lines. ADRM1 knockdown significantly suppressed HL60 cell proliferation (48.82 ± 12.58%) and colony formation and caused cell cycle arrest in the G0/G1 phase. Furthermore, we confirmed that ADRM1 knockdown suppressed p65 nuclear translocation.
  
  
  CONCLUSIONS
  Our study revealed that ADRM1 was overexpressed in AL, especially in CD34+ leukemia stem and progenitor cells. ADRM1 may play a role in AL via the proteasome-ubiquitin pathway by potentially sustaining the activation of NF-κB signaling.
authors.affiliation: Union Clinical Medical College, Fujian Medical University, Fujian Medical University Union Hospital, Fuzhou, PR China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Zheng
authors.forename: Yafei
authors.initials: Y
authors.lastname: Guo
authors.forename: Yingying
authors.initials: Y
authors.lastname: Chen
authors.forename: Meilin
authors.initials: M
authors.lastname: Chen
authors.forename: Zhenxin
authors.initials: Z
authors.lastname: Lin
authors.forename: Yong
authors.initials: Y
authors.lastname: Wu
authors.forename: Yuanzhong
authors.initials: Y
authors.lastname: Chen
mesh_terms: D018952:Antigens, CD34 / Q000378:metabolism; D017209:Apoptosis; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D016193:G1 Phase; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D018922:HL-60 Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D007938:Leukemia / Q000097:blood / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D015473:Leukemia, Promyelocytic, Acute / Q000097:blood / Q000378:metabolism* / Q000473:pathology / Q000628:therapy; D007963:Leukocytes, Mononuclear / Q000378:metabolism / Q000473:pathology; D008562:Membrane Glycoproteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D009363:Neoplasm Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering; D016192:Resting Phase, Cell Cycle; D014407:Tumor Cells, Cultured; D014410:Tumor Stem Cell Assay
chemical_list: C500781:ADRM1 protein, human; D018952:Antigens, CD34; D047908:Intracellular Signaling Peptides and Proteins; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins; D034741:RNA, Small Interfering