title: In Vitro Production of Fumonisins by Fusarium verticillioides under Oxidative Stress Induced by H2O2.
abstract: The effects of oxidative stress induced by H2O2 were tested in liquid cultures in the fumonisin-producing fungus Fusarium verticillioides. The quantitative analysis of fumonisins B1, B2, B3, and B4 was achieved by means of liquid chromatography coupled to high-resolution mass spectrometry. Two effects in F. verticillioides, consisting of different abilities to produce fumonisins in response to oxidative stress, were identified. Following H2O2 addition, two F. verticillioides strains produced significantly more fumonisin (>300%) while three other strains produced significantly less (<20%) in comparison to control cultures. Transcriptional studies with seven biosynthetic genes showed a significant increase in transcript levels in the strain that made more fumonisin and either no or minimal changes in the strain that made less fumonisin. Our data indicate the important role of oxidative stress toward the modulation of the fumonisin biosynthesis and suggest the necessity to verify the presence of such divergent behavior in F. verticillioides populations under natural conditions.
authors.affiliation: †Department of Land, Environment, Agriculture and Forestry, University of Padua, Campus of Agripolis, Viale Università 16, 35020 Legnaro, Padua, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Ferrigo
authors.affiliation: †Department of Land, Environment, Agriculture and Forestry, University of Padua, Campus of Agripolis, Viale Università 16, 35020 Legnaro, Padua, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Raiola
authors.affiliation: ‡Department of Chemical Science, University of Padua, via Marzolo 1, 35131 Padua, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Bogialli
authors.affiliation: ‡Department of Chemical Science, University of Padua, via Marzolo 1, 35131 Padua, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Bortolini
authors.affiliation: ‡Department of Chemical Science, University of Padua, via Marzolo 1, 35131 Padua, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Tapparo
authors.affiliation: †Department of Land, Environment, Agriculture and Forestry, University of Padua, Campus of Agripolis, Viale Università 16, 35020 Legnaro, Padua, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Causin
mesh_terms: D037341:Fumonisins / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D005670:Fusarium / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*
chemical_list: D037341:Fumonisins; D005656:Fungal Proteins; D006861:Hydrogen Peroxide
keywords: Fusarium verticillioides; H2O2; LC-high-resolution MS analysis; fum genes; fumonisins; oxidative stress