title: Nanoparticle-mediated decrease of lamin B1 pools promotes a TRF protein-based adaptive response in cultured cells.
abstract: In general, nanoparticle-based materials are promising candidates for use in biological systems for diagnostic and therapeutic approaches. However, these materials' actions at the molecular level remain poorly understood. Nanoparticle (silica, silver and diamond)-induced oxidative stress and activation of the NF-κB pathway lead to the depletion of lamin B1 pools, which, in turn, results in upregulation of telomeric repeat binding factor (TRF) protein expression and maintenance of telomere length. In cancer cells, the TRF-based response is independent of the p53 pathway. In fibroblasts with active p53/p21 signaling, the levels of p53 and p21 are elevated and stress-induced premature senescence is observed. These results suggest that nanoparticles promote a telomere-focused cell adaptive response.
authors.affiliation: Department of Genetics, University of Rzeszow, Rejtana 16C, 35-959 Rzeszow, Poland.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Mytych
authors.affiliation: Department of Genetics, University of Rzeszow, Rejtana 16C, 35-959 Rzeszow, Poland.
authors.forename: Karolina
authors.initials: K
authors.lastname: Pacyk
authors.affiliation: Department of Genetics, University of Rzeszow, Rejtana 16C, 35-959 Rzeszow, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Pepek
authors.affiliation: Department of Plant Physiology, University of Rzeszow, Werynia 502, 36-100 Kolbuszowa, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Zebrowski
authors.affiliation: Department of Biochemistry and Cell Biology, University of Rzeszow, Zelwerowicza 4, 35-601 Rzeszow, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Lewinska
authors.affiliation: Department of Genetics, University of Rzeszow, Rejtana 16C, 35-959 Rzeszow, Poland. Electronic address: mawnuk@gmail.com.
authors.forename: Maciej
authors.initials: M
authors.lastname: Wnuk
mesh_terms: D000222:Adaptation, Physiological; D002478:Cells, Cultured; D006801:Humans; D034921:Lamin Type B / Q000378:metabolism*; D053758:Nanoparticles; D018384:Oxidative Stress; D035182:TATA Box Binding Protein-Like Proteins / Q000378:metabolism*
chemical_list: D034921:Lamin Type B; D035182:TATA Box Binding Protein-Like Proteins; C441409:TBPL1 protein, human
keywords: Adaptive response; Lamin B1; Nanoparticles; Oxidative stress; TRF proteins; Telomere length