title: Nutritional recovery with a soybean diet after weaning reduces lipogenesis but induces inflammation in the liver in adult rats exposed to protein restriction during intrauterine life and lactation.
abstract: We evaluated the effects of postweaning nutritional recovery with a soybean flour diet on de novo hepatic lipogenesis and inflammation in adult rats exposed to protein restriction during intrauterine life and lactation. Rats from mothers fed with protein (casein) in a percentage of 17% (control, C) or 6% (low, L) during pregnancy and lactation were fed with diet that contained 17% casein (CC and LC groups, resp.) or soybean (CS and LS groups, resp.) after weaning until 90 days of age. LS and CS rats had low body weight, normal basal serum triglyceride levels, increased ALT concentrations, and high HOMA-IR indices compared with LC and CC rats. The soybean diet reduced PPARγ as well as malic enzyme and citrate lyase contents and activities. The lipogenesis rate and liver fat content were lower in LS and CS rats relative to LC and CC rats. TNFα mRNA and protein levels were higher in LS and CS rats than in LC and CC rats. NF-κB mRNA levels were lower in the LC and LS groups compared with the CC and LC groups. Thus, the soybean diet prevented hepatic steatosis at least in part through reduced lipogenesis but resulted in TNFα-mediated inflammation.
authors.affiliation: Departamento de Alimentos e Nutrição, Faculdade de Nutrição, Universidade Federal de Mato Grosso (UFMT), 78060-900 Cuiabá, MT, Brazil.
authors.forename: Sílvia Regina de Lima
authors.initials: SR
authors.lastname: Reis
authors.affiliation: Departamento de Alimentos e Nutrição, Faculdade de Nutrição, Universidade Federal de Mato Grosso (UFMT), 78060-900 Cuiabá, MT, Brazil.
authors.forename: Naoel Hassan
authors.initials: NH
authors.lastname: Feres
authors.affiliation: Departamento de Alimentos e Nutrição, Faculdade de Nutrição, Universidade Federal de Mato Grosso (UFMT), 78060-900 Cuiabá, MT, Brazil.
authors.forename: Leticia Martins
authors.initials: LM
authors.lastname: Ignacio-Souza
authors.affiliation: Departamento de Alimentos e Nutrição, Faculdade de Nutrição, Universidade Federal de Mato Grosso (UFMT), 78060-900 Cuiabá, MT, Brazil.
authors.forename: Roberto Vilela
authors.initials: RV
authors.lastname: Veloso
authors.affiliation: Departamento de Alimentos e Nutrição, Faculdade de Nutrição, Universidade Federal de Mato Grosso (UFMT), 78060-900 Cuiabá, MT, Brazil.
authors.forename: Vanessa Cristina
authors.initials: VC
authors.lastname: Arantes
authors.affiliation: Departamento de Química, Instituto de Ciências Exatas e da Terra, Universidade Federal de Mato Grosso (UFMT), 78060-900 Cuiabá, MT, Brazil.
authors.forename: Nair Honda
authors.initials: NH
authors.lastname: Kawashita
authors.affiliation: Departamento de Clínica Médica Veterinária, Faculdade de Agronomia e Medicina Veterinária, Universidade Federal de Mato Grosso (UFMT), 78060-900 Cuiabá, MT, Brazil.
authors.forename: Edson Moleta
authors.initials: EM
authors.lastname: Colodel
authors.affiliation: Departamento de Alimentos e Nutrição, Faculdade de Nutrição, Universidade Federal de Mato Grosso (UFMT), 78060-900 Cuiabá, MT, Brazil.
authors.forename: Bárbara Laet
authors.initials: BL
authors.lastname: Botosso
authors.affiliation: Departamento de Alimentos e Nutrição, Faculdade de Nutrição, Universidade Federal de Mato Grosso (UFMT), 78060-900 Cuiabá, MT, Brazil.
authors.forename: Marise Auxiliadora de Barros
authors.initials: MA
authors.lastname: Reis
authors.affiliation: Departamento de Alimentos e Nutrição, Faculdade de Nutrição, Universidade Federal de Mato Grosso (UFMT), 78060-900 Cuiabá, MT, Brazil.
authors.forename: Márcia Queiroz
authors.initials: MQ
authors.lastname: Latorraca
mesh_terms: D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D018753:Diet, Protein-Restricted; D005260:Female; D006706:Homeostasis; D007249:Inflammation / Q000473:pathology*; D007328:Insulin / Q000097:blood; D007774:Lactation; D008055:Lipids / Q000097:blood; D050155:Lipogenesis; D008066:Lipolysis; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009097:Multienzyme Complexes / Q000737:chemistry; D016328:NF-kappa B / Q000378:metabolism; D007652:Oxo-Acid-Lyases / Q000737:chemistry; D047495:PPAR gamma / Q000378:metabolism; D011247:Pregnancy; D039401:Prenatal Nutritional Physiological Phenomena; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013025:Glycine max / Q000737:chemistry*; D051780:Sterol Regulatory Element Binding Protein 1 / Q000378:metabolism; D014886:Weaning
chemical_list: D007328:Insulin; D008055:Lipids; D009097:Multienzyme Complexes; D016328:NF-kappa B; D047495:PPAR gamma; D012333:RNA, Messenger; D051780:Sterol Regulatory Element Binding Protein 1; D007652:Oxo-Acid-Lyases; C019909:citrate (pro-3S)-lyase