title: [GLP-1 receptor activation effects the p38MAPK signal pathway in hepatic stellate cells].
abstract: OBJECTIVE
  To investigate the effects of activation of the GLP-1 receptor on the p38MAPK signaling pathway in hepatic stellate cells (HSCs).
  
  
  METHODS
  HSCs were isolated and identified according to morphological features; the levels of GLP-1R protein were determined by western blotting.The HSCs were randomly divided into a control grouP (normal saline treatment) and experimental grouP(liraglutide treatment); after 120 hours, the expression of p38MAPK mRNA was examined by RT-PCR and of phosphorylated (p)-p38MAPK protein was detected by western blotting.
  
  
  RESULTS
  GLP-1R proteins were detected in the HSCs. Compared with the control group, the experimental group showed significantly decreased p38MAPK mRNA and p-p38MAPK protein (both P < 0.01).
  
  
  CONCLUSIONS
  The p38MAPK signaling pathway could be down-regulated when GLP-1R is activated in HSCs.
authors.affiliation: Department of Hepatology, the Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310005, China.
authors.forename: Lingkang
authors.initials: L
authors.lastname: Wu
authors.forename: Youming
authors.initials: Y
authors.lastname: Li
authors.forename: Yingchao
authors.initials: Y
authors.lastname: Liu
authors.forename: Cuilan
authors.initials: C
authors.lastname: Tang
authors.forename: Feng
authors.initials: F
authors.lastname: Wu
authors.forename: Liangliang
authors.initials: L
authors.lastname: Shi
authors.forename: Keda
authors.initials: K
authors.lastname: Lu
mesh_terms: D002478:Cells, Cultured; D052216:Glucagon-Like Peptide 1 / Q000031:analogs & derivatives / Q000494:pharmacology; D000067757:Glucagon-Like Peptide-1 Receptor; D055166:Hepatic Stellate Cells / Q000378:metabolism*; D006801:Humans; D000069450:Liraglutide; D020935:MAP Kinase Signaling System; D012333:RNA, Messenger; D018027:Receptors, Glucagon / Q000378:metabolism*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: C000593833:GLP1R protein, human; D000067757:Glucagon-Like Peptide-1 Receptor; D012333:RNA, Messenger; D018027:Receptors, Glucagon; D000069450:Liraglutide; D052216:Glucagon-Like Peptide 1; D048051:p38 Mitogen-Activated Protein Kinases