title: In vitro and in vivo protein uptake studies in plant mitochondria.
abstract: The study of protein uptake into mitochondria is an important tool for investigating the subcellular distribution of proteins and the molecular mechanisms that determine location. Here we describe five techniques that allow the quantitative or qualitative monitoring of protein uptake into mitochondria using both in vitro and in vivo approaches.
authors.affiliation: ARC Centre of Excellence in Plant Energy Biology, The University of Western Australia, Bayliss Building M316, 35 Stirling Highway, Crawley, WA, 6009, Australia.
authors.forename: Owen
authors.initials: O
authors.lastname: Duncan
authors.forename: Chris
authors.initials: C
authors.lastname: Carrie
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Monika W
authors.initials: MW
authors.lastname: Murcha
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005453:Fluorescence; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007553:Isotope Labeling / Q000379:methods; D008928:Mitochondria / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D010944:Plants / Q000378:metabolism*; D021381:Protein Transport
chemical_list: D010940:Plant Proteins; D049452:Green Fluorescent Proteins