title: Effects of tributyltin chloride on developing mouse oocytes and preimplantation embryos.
abstract: Tributyltin, an organotin, is ubiquitous in estuaries and freshwater systems. Previous reports suggest that tributyltin is an endocrine disruptor in many wildlife species and it inhibits aromatase in mammalian placental and granulosa-like tumor cell lines. However, no evidence showing the effects of tributyltin on oocytes or preimplantation embryonic developmental competence exists. Therefore, we investigated the role of tributyltin chloride (TBTCl) in the development of female oocytes and preimplantation embryos. Briefly, female ICR mice were gavaged with 0 (vehicle), 4, and 8 mg/kg of TBTCl each day for 18 days. The fluorescence intensity analysis showed that the 5-methylcytosine level decreased after TBTCl treatment, indicating that the general DNA methylation level decreased in the treated oocytes. Our results demonstrate that TBTCl treatment results in decreased mRNA levels of imprinted genes H19, Igf2r, and Peg3 during oocyte growth. The TBTCl-treated oocytes showed a significant increase in reactive oxygen species levels in germinal vesicle oocytes. In TBTCl-treated oocytes, there was no difference in GPx and Sod1 expression, but a decreased mRNA level of Cat occurred when compared with control. Moreover, the blastocysts with TBTCl exposure displayed higher apoptotic signals. These results suggest that TBTCl induces developmental defects in oocytes and preimplantation embryos.
authors.affiliation: College of Animal Science and Technology,Nanjing Agricultural University,Weigang No. 1,Nanjing 210095,China.
authors.forename: Xian-Ju
authors.initials: XJ
authors.lastname: Huang
authors.affiliation: College of Animal Science and Technology,Nanjing Agricultural University,Weigang No. 1,Nanjing 210095,China.
authors.forename: Ming
authors.initials: M
authors.lastname: Shen
authors.affiliation: College of Animal Science and Technology,Nanjing Agricultural University,Weigang No. 1,Nanjing 210095,China.
authors.forename: Lizhong
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology,Nanjing Agricultural University,Weigang No. 1,Nanjing 210095,China.
authors.forename: Fengxiang
authors.initials: F
authors.lastname: Yu
authors.affiliation: College of Animal Science and Technology,Nanjing Agricultural University,Weigang No. 1,Nanjing 210095,China.
authors.forename: Wangjun
authors.initials: W
authors.lastname: Wu
authors.affiliation: College of Animal Science and Technology,Nanjing Agricultural University,Weigang No. 1,Nanjing 210095,China.
authors.forename: Hong-Lin
authors.initials: HL
authors.lastname: Liu
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000187:drug effects*; D052244:Endocrine Disruptors / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D008813:Mice, Inbred ICR; D009865:Oocytes / Q000187:drug effects*; D009947:Organotin Compounds / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D017382:Reactive Oxygen Species / Q000032:analysis; D014220:Trialkyltin Compounds / Q000378:metabolism*
chemical_list: D052244:Endocrine Disruptors; D009947:Organotin Compounds; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D014220:Trialkyltin Compounds; C011559:tributyltin
keywords: defects