title: [DNA aptamer based sorbents for binding human IgE].
abstract: DNA aptamer based sorbents are synthesized for binding human IgE. Sorbents effectively removed IgE from human blood plasma. The experimental values of IgE desorption constants were from 11 x 10(-l0) to 1.7 x 10(-10) M depending on the orientation of the aptamer, an insoluble matrix. The sorbents were stable during multiple use. Conditions for sorbent regeneration were picked up. These chromatographic materials can be used for medical and biotechnological applications.
authors.forename: V A
authors.initials: VA
authors.lastname: Spiridonova
authors.forename: P A
authors.initials: PA
authors.lastname: Levashov
authors.forename: E D
authors.initials: ED
authors.lastname: Ovchinnikova
authors.forename: O I
authors.initials: OI
authors.lastname: Afanas'eva
authors.forename: K A
authors.initials: KA
authors.lastname: Glinkina
authors.forename: I Iu
authors.initials: IIu
authors.lastname: Adamova
authors.forename: S N
authors.initials: SN
authors.lastname: Pokrovskiĭ
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D002845:Chromatography; D004247:DNA / Q000737:chemistry; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000737:chemistry* / Q000302:isolation & purification; D009841:Oligonucleotides / Q000737:chemistry*; D011485:Protein Binding
chemical_list: D052157:Aptamers, Nucleotide; D009841:Oligonucleotides; D007073:Immunoglobulin E; D004247:DNA