title: In situ single step detection of exosome microRNA using molecular beacon.
abstract: In situ single step detection of microRNAs (miRNA) in a whole exosome has been developed as a novel diagnosis method that can be utilized for various diseases. Exosomes are small extracellular vesicles that contain biomarker miRNAs produced from their originating cells and are known to travel through the circulatory system. This makes exosomal miRNAs from the body fluids an attractive biomarker that can lead to a paradigm shift in the diagnosis of disease. However, current techniques, including real-time PCR analysis, are time-consuming and laborious, making them unsuitable for exosomal miRNA detection for diagnosis. Thus, the development of alternative methods is necessary. Herein, we have demonstrated that exosomal miRNAs can be detected directly using a nano-sized fluorescent oligonucleotide probe, molecular beacon. MiRNA-21 in exosomes from breast cancer cells were detected successfully by molecular beacons in a quantitative manner. Permeabilization by streptolysin O treatment further enhanced the delivery of molecular beacons into exosomes, giving significantly increased signals from target miRNAs. In addition, we selectively detected cancer cell-derived exosomal miRNA-21 among heterogeneous exosome mixtures and in human serum. The method developed in the article is simple, fast, and sensitive, so it will offer great opportunities for the high-throughput diagnosis and prognosis of diseases.
authors.affiliation: Division of Bioengineering, Incheon National University, Incheon 406-772, Republic of Korea.
authors.forename: Ji Hye
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Jeong Ah
authors.initials: JA
authors.lastname: Kim
authors.affiliation: Division of Bioengineering, Incheon National University, Incheon 406-772, Republic of Korea.
authors.forename: Min Hee
authors.initials: MH
authors.lastname: Kwon
authors.affiliation: Center for BioMicrosystems, Brain Science Institute, Korea Institute of Science and Technology (KIST), Seoul, Republic of Korea.
authors.forename: Ji Yoon
authors.initials: JY
authors.lastname: Kang
authors.affiliation: Division of Bioengineering, Incheon National University, Incheon 406-772, Republic of Korea. Electronic address: wjrhee@incheon.ac.kr.
authors.forename: Won Jong
authors.initials: WJ
authors.lastname: Rhee
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000235:genetics*; D063326:Exosome Multienzyme Ribonuclease Complex / Q000032:analysis / Q000235:genetics*; D006801:Humans; D061986:MCF-7 Cells; D035683:MicroRNAs / Q000032:analysis / Q000235:genetics*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D015336:Molecular Probe Techniques; D015335:Molecular Probes / Q000235:genetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; C524940:MIRN21 microRNA, human; D035683:MicroRNAs; D015335:Molecular Probes; D063326:Exosome Multienzyme Ribonuclease Complex
keywords: Cancer; Diagnosis; Exosome; Molecular beacon; microRNA