title: Easy storage strategies for Sporothrix spp. strains.
abstract: The present study evaluated the maintenance of Sporothrix spp. (6 Sporothrix brasiliensis; 6 S. schenckii; 5 S. mexicana, and 3 S. globosa) in saline at 4°C, and in 10% glycerol plus either 10% lactose or 10% sucrose, at -20°C and -80°C. Viability was assessed after 3, 6, and 9 months of storage, through the recovery of strains on potato dextrose agar and analysis of macro- and micromorphological features. Conidium quantification was performed before and after storage, at 3, 6 and 9 months. 100% viability was observed, regardless of storage conditions or time period. Storage at 4°C and at -20°C did not alter the number of conidia, but lower conidium counts were observed at -80°C. This study shows that the combination of glycerol with lactose or sucrose is effective to maintain Sporothrix spp. at freezing temperatures.
authors.affiliation: 1 Specialized Medical Mycology Center, Graduate Program in Medical Microbiology, Federal University of Ceará , Fortaleza, Ceará, Brazil .
authors.forename: Raimunda Sâmia Nogueira
authors.initials: RS
authors.lastname: Brilhante
authors.forename: Natalya Fechine
authors.initials: NF
authors.lastname: Silva
authors.forename: Rita Amanda Chaves de
authors.initials: RA
authors.lastname: Lima
authors.forename: Érica Pacheco
authors.initials: ÉP
authors.lastname: Caetano
authors.forename: Lucas Pereira de
authors.initials: LP
authors.lastname: Alencar
authors.forename: Débora de Souza Collares Maia
authors.initials: Dde S
authors.lastname: Castelo-Branco
authors.forename: José Luciano Bezerra
authors.initials: JL
authors.lastname: Moreira
authors.forename: Silviane Praciano
authors.initials: SP
authors.lastname: Bandeira
authors.forename: Zoilo Pires de
authors.initials: ZP
authors.lastname: Camargo
authors.forename: Anderson Messias
authors.initials: AM
authors.lastname: Rodrigues
authors.forename: Tereza de Jesus Pinheiro Gomes
authors.initials: Tde J
authors.lastname: Bandeira
authors.forename: André Jalles
authors.initials: AJ
authors.lastname: Monteiro
authors.forename: Rossana de Aguiar
authors.initials: Rde A
authors.lastname: Cordeiro
authors.forename: José Júlio Costa
authors.initials: JJ
authors.lastname: Sidrim
authors.forename: Marcos Fábio Gadelha
authors.initials: MF
authors.lastname: Rocha
mesh_terms: D015925:Cryopreservation; D005990:Glycerol / Q000737:chemistry; D007785:Lactose / Q000737:chemistry; D050296:Microbial Viability; D011309:Preservation, Biological / Q000379:methods*; D013175:Sporothrix / Q000737:chemistry / Q000502:physiology*; D013395:Sucrose / Q000737:chemistry; D013995:Time
chemical_list: D013395:Sucrose; D007785:Lactose; D005990:Glycerol