title: Single-band upconversion nanoprobes for multiplexed simultaneous in situ molecular mapping of cancer biomarkers.
abstract: The identification of potential diagnostic markers and target molecules among the plethora of tumour oncoproteins for cancer diagnosis requires facile technology that is capable of quantitatively analysing multiple biomarkers in tumour cells and tissues. Diagnostic and prognostic classifications of human tumours are currently based on the western blotting and single-colour immunohistochemical methods that are not suitable for multiplexed detection. Herein, we report a general and novel method to prepare single-band upconversion nanoparticles with different colours. The expression levels of three biomarkers in breast cancer cells were determined using single-band upconversion nanoparticles, western blotting and immunohistochemical technologies with excellent correlation. Significantly, the application of antibody-conjugated single-band upconversion nanoparticle molecular profiling technology can achieve the multiplexed simultaneous in situ biodetection of biomarkers in breast cancer cells and tissue specimens and produce more accurate results for the simultaneous quantification of proteins present at low levels compared with classical immunohistochemical technology.
authors.affiliation: Department of Chemistry and Laboratory of Advanced Materials, iChEm (Collaborative Innovation Center of Chemistry for Energy Materials), State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai 200433, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Chemistry, iChEm (Collaborative Innovation Center of Chemistry for Energy Materials), State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai 200433, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Chemistry, iChEm (Collaborative Innovation Center of Chemistry for Energy Materials), State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai 200433, China.
authors.forename: Chi
authors.initials: C
authors.lastname: Yao
authors.affiliation: Department of Chemistry, iChEm (Collaborative Innovation Center of Chemistry for Energy Materials), State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai 200433, China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Chemistry, iChEm (Collaborative Innovation Center of Chemistry for Energy Materials), State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai 200433, China.
authors.forename: Chengli
authors.initials: C
authors.lastname: Wang
authors.affiliation: Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200011, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200011, China.
authors.forename: Congjian
authors.initials: C
authors.lastname: Xu
authors.affiliation: Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800, China.
authors.forename: Aijun
authors.initials: A
authors.lastname: Zeng
authors.affiliation: Department of Chemistry, iChEm (Collaborative Innovation Center of Chemistry for Energy Materials), State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai 200433, China.
authors.forename: Dongyuan
authors.initials: D
authors.lastname: Zhao
authors.affiliation: Department of Chemistry, iChEm (Collaborative Innovation Center of Chemistry for Energy Materials), State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai 200433, China.
authors.forename: Fan
authors.identifier: 0000000178866144
authors.initials: F
authors.lastname: Zhang
mesh_terms: D014408:Biomarkers, Tumor; D001943:Breast Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D003952:Diagnostic Imaging / Q000379:methods*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D028581:Lanthanoid Series Elements / Q000737:chemistry; D015394:Molecular Structure; D049329:Nanostructures
chemical_list: D014408:Biomarkers, Tumor; D028581:Lanthanoid Series Elements