title: Delayed Effect of the Light Pulse on Phosphorylated ERK1/2 and GSK3β Kinases in the Ventrolateral Suprachiasmatic Nucleus of Rat.
abstract: The intrinsic period of circadian clock in the suprachiasmatic nucleus is entrained to a 24-h cycle by external cues, mainly light. Previous studies have shown that light applied at night induces robust phosphorylation of extracellular-signal-regulated kinase that is necessary to process the light pulse into the phase shift of the clock phase. In this study, we show the persistent downregulation of phosphorylated extracellular-signal-regulated kinase and transient downregulation of phosphorylated glycogen synthase kinase-3beta in the ventrolateral part of the suprachiasmatic nucleus to photic stimuli starting at 2 h after the beginning of the light pulse. As both kinases are involved in regulation of circadian clockwork, we hypothesize that these changes may contribute to the phase-shifting effect of light at night.
authors.affiliation: Department of Physiology, Faculty of Science, Charles University in Prague, Viničná 7, Prague 2, 128 43, Czech Republic.
authors.forename: Kateřina
authors.initials: K
authors.lastname: Červená
authors.forename: Dominika
authors.initials: D
authors.lastname: Pačesová
authors.forename: Veronika
authors.initials: V
authors.lastname: Spišská
authors.forename: Zdeňka
authors.initials: Z
authors.lastname: Bendová
mesh_terms: D000818:Animals; D057906:Circadian Clocks; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism*; D000071679:Glycogen Synthase Kinase 3 beta; D020935:MAP Kinase Signaling System; D008297:Male; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism*; D010775:Photic Stimulation; D051381:Rats; D017208:Rats, Wistar; D011930:Reaction Time; D013493:Suprachiasmatic Nucleus / Q000378:metabolism* / Q000502:physiology
chemical_list: D000071679:Glycogen Synthase Kinase 3 beta; C000605508:Gsk3b protein, rat; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D038362:Glycogen Synthase Kinase 3