title: Binding of Substrate Locks the Electrochemistry of CRY-DASH into DNA Repair.
abstract: VcCry1, a member of the CRY-DASH family, may serve two diverse roles in vivo, including blue-light signaling and repair of UV-damaged DNA. We have discovered that the electrochemistry of the flavin adenine dinucleotide cofactor of VcCry1 is locked to cycle only between the hydroquinone and neutral semiquinone states when UV-damaged DNA is present. Other potential substrates, including undamaged DNA and ATP, have no discernible effect on the electrochemistry, and the kinetics of the reduction is unaffected by damaged DNA. Binding of the damaged DNA substrate determines the role of the protein and prevents the presumed photochemistry required for blue-light signaling.
authors.affiliation: Department of Chemistry and Biochemistry, Montclair State University, Montclair, New Jersey 07043, United States.
authors.forename: Yvonne M
authors.initials: YM
authors.lastname: Gindt
authors.affiliation: Department of Chemistry and Biochemistry, Montclair State University, Montclair, New Jersey 07043, United States.
authors.forename: Adriana
authors.initials: A
authors.lastname: Messyasz
authors.affiliation: Department of Chemistry and Biochemistry, Montclair State University, Montclair, New Jersey 07043, United States.
authors.forename: Pamela I
authors.initials: PI
authors.lastname: Jumbo
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry*; D056931:Cryptochromes / Q000737:chemistry*; D004260:DNA Repair; D004269:DNA, Bacterial / Q000737:chemistry / Q000528:radiation effects; D004563:Electrochemistry; D005182:Flavin-Adenine Dinucleotide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010084:Oxidation-Reduction; D011485:Protein Binding; D011740:Pyrimidine Dimers / Q000737:chemistry; D013816:Thermodynamics; D014466:Ultraviolet Rays; D014734:Vibrio cholerae / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D056931:Cryptochromes; D004269:DNA, Bacterial; D011740:Pyrimidine Dimers; D005182:Flavin-Adenine Dinucleotide; D000255:Adenosine Triphosphate