title: Simultaneous electrochemical detection of multiple biomarkers using gold nanoparticles decorated multiwall carbon nanotubes as signal enhancers.
abstract: In this work, a novel sandwich-type electrochemical immunosensor has been developed for simultaneous detection of carcinoembryonic antigen (CEA) and α-fetoprotein (AFP) based on metal ion labels. Gold nanoparticles decorated multiwall carbon nanotubes (AuNPs@MWCNTs) were used as carriers to immobilize secondary antibodies and distinguishable electrochemical tags of Pb(2+) and Cd(2+) to amplify the signals. Due to the intrinsic property of high surface-to-volume ratio, the AuNPs@MWCNTs could load numerous secondary antibodies and labels. Therefore, the multiplexed immunoassay exhibited good sensitivity and selectivity. Experimental results revealed that this sandwich-type immunoassay displayed an excellent linear response, with a linear range of 0.01 to 60 ng mL(-1) for both analytes and detection limits of 3.0 pg mL(-1) for CEA and 4.5 pg mL(-1) for AFP (at a signal-to-noise ratio of 3). The method was successfully applied for the determination of AFP and CEA levels in clinical serum samples.
authors.affiliation: College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, People's Republic of China; Department of Chemistry, Wannan Medical College, Wuhu 241002, People's Republic of China.
authors.forename: Dexiang
authors.initials: D
authors.lastname: Feng
authors.affiliation: College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, People's Republic of China; Department of Chemistry, Wannan Medical College, Wuhu 241002, People's Republic of China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Li
authors.affiliation: College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, People's Republic of China.
authors.forename: Junqing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, People's Republic of China. Electronic address: zhyz65@mail.ahnu.edu.cn.
authors.forename: Yuzhong
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry*; D015415:Biomarkers / Q000032:analysis / Q000097:blood; D002104:Cadmium / Q000737:chemistry; D002272:Carcinoembryonic Antigen / Q000097:blood*; D002413:Cations, Divalent / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007854:Lead / Q000737:chemistry; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D000509:alpha-Fetoproteins / Q000032:analysis*
chemical_list: D055910:Antibodies, Immobilized; D015415:Biomarkers; D002272:Carcinoembryonic Antigen; D002413:Cations, Divalent; D037742:Nanotubes, Carbon; D000509:alpha-Fetoproteins; D002104:Cadmium; D007854:Lead; D006046:Gold
keywords: Carcinoembryonic antigen; Gold nanoparticles decorated multiwall carbon nanotubes; Multiplex assay; Pb(2+) and Cd(2+); α-Fetoprotein