title: Potential risk of misclassification HCV 2k/1b strains as HCV 2a/2c using VERSANT HCV Genotype 2.0 assay.
abstract: The performance of a hepatitis C virus (HCV) NS5B sequencing method described by Murphy et al. (Use of sequence analysis of the NS5B region for routine genotyping of hepatitis C virus with reference to C/E1 and 5' untranslated region sequences. J Clin Microbiol 2007;45(4):1102-12.) was compared with the VERSANT HCV Genotype 2.0 assay. The sequencing strategy led to detection of HCV recombinant genotype 2k/1b, previously identified as genotype 2a/2c, which reveals the importance of exact HCV genotyping and subtyping.
authors.affiliation: Department of Laboratory Medicine, AZ St-Jan Bruges, Bruges, Belgium. Electronic address: stevendekeukeleire@hotmail.com.
authors.forename: Steven
authors.initials: S
authors.lastname: De Keukeleire
authors.affiliation: Department of Laboratory Medicine, AZ St-Jan Bruges, Bruges, Belgium.
authors.forename: Patrick
authors.initials: P
authors.lastname: Descheemaeker
authors.affiliation: Department of Laboratory Medicine, AZ St-Jan Bruges, Bruges, Belgium.
authors.forename: Marijke
authors.initials: M
authors.lastname: Reynders
mesh_terms: D003951:Diagnostic Errors; D005838:Genotype; D060005:Genotyping Techniques / Q000379:methods*; D016174:Hepacivirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D006526:Hepatitis C / Q000175:diagnosis* / Q000821:virology*; D006801:Humans
keywords: HCV 2k/1b; HCV genotyping and subtyping; Hepatitis C virus; Recombinant forms