title: Oridonin alters the expression profiles of microRNAs in BxPC-3 human pancreatic cancer cells.
abstract: BACKGROUND
  Oridonin, an ingredient used in traditional Chinese medicine, has been demonstrated to play an important role in antitumour effects, but the mechanism underlying its antitumour properties is still not clear.
  
  
  METHODS
  To verify the anti-cancer effects of oridonin via a miRNA-dependent mechanism, comprehensive miRNA expression profiling of oridonin-treated BxPC-3 human pancreatic cancer cells was performed using a miRNA microarray assay based on Sanger miR-Base Release 20, followed by a validation using real-time PCR. MicroRNA target prediction and Gene Ontology and KEGG pathway analysis were performed to investigate possible pathways involved.
  
  
  RESULTS
  The results showed that 105 miRNAs were significantly differentially expressed (signal reading >500, p ≤ 0.01, |Log2-value| ≥1) in oridonin-treated BxPC-3 human pancreatic cancer cells.
  
  
  CONCLUSIONS
  Our data indicates that oridonin inhibits BxPC-3 cells probably through regulating the expression of miRNAs. Interruption of miRNA profiling may provide new therapeutic methods for the clinical treatment of pancreatic cancer.
authors.affiliation: Medical Technology College, Zhejiang Chinese Medical University, Binwen Road, Binjiang District, Hangzhou, 310053, Zhejiang Province, China. 934309858@qq.com.
authors.forename: Zhifang
authors.initials: Z
authors.lastname: Gui
authors.affiliation: Medical Technology College, Zhejiang Chinese Medical University, Binwen Road, Binjiang District, Hangzhou, 310053, Zhejiang Province, China. 1522716800@qq.com.
authors.forename: Shuquan
authors.initials: S
authors.lastname: Li
authors.affiliation: School of Medicine, Jinggangshan University, Ji'an, 343000, China. bjxj2005@163.com.
authors.forename: Xing
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of General Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, 310016, China. xubinmd@163.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Xu
authors.affiliation: Medical Technology College, Zhejiang Chinese Medical University, Binwen Road, Binjiang District, Hangzhou, 310053, Zhejiang Province, China. xujian832002@163.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Xu
mesh_terms: D045786:Diterpenes, Kaurane / Q000494:pharmacology* / Q000627:therapeutic use; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D037602:Isodon / Q000737:chemistry*; D035683:MicroRNAs / Q000378:metabolism*; D010190:Pancreatic Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D008517:Phytotherapy; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D045786:Diterpenes, Kaurane; D004365:Drugs, Chinese Herbal; D035683:MicroRNAs; C011959:oridonin