title: Cell-selective encapsulation in hydrogel sheaths via biospecific identification and biochemical cross-linking.
abstract: Selective encapsulation of a particular cell population from heterogeneous cell populations has potential applications such as studies in cell-to-cell communication, regenerative medicine, and cell therapies. However, there are no versatile methods for realizing this. Here we report a method based on biospecific identification of the target cells through antigen-antibody reaction and subsequent enzymatic hydrogel sheath formation on the cell surfaces by horseradish peroxidase (HRP). Human hepatoma cell line HepG2 cells were selectively encapsulated in alginate-based hydrogel sheath from the mixture with mouse embryo fibroblast-like cell line 10T1/2 fibroblasts using anti-human CD326 antibody conjugated with HRP. The viability of the encapsulated cells was 93%. The cells released at 6 days of the encapsulation by degrading the sheath using alginate lyase grew almost the same as those free from encapsulation. The versatility of the method was confirmed using another antibody, cells, and hydrogel sheath material: Only human vein endothelial cells were encapsulated in gelatin-based hydrogel sheath from the mixture with 10T1/2 fibroblasts using anti-human CD31 antibody conjugated with HRP. The cell-selective encapsulation was also achieved by a system using a primary antibody with a secondary antibody conjugated with HRP.
authors.affiliation: Department of Materials Science and Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan. Electronic address: sakai@cheng.es.osaka-u.ac.jp.
authors.forename: Shinji
authors.initials: S
authors.lastname: Sakai
authors.affiliation: Department of Materials Science and Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Materials Science and Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.
authors.forename: Mikako
authors.initials: M
authors.lastname: Sengoku
authors.affiliation: Department of Materials Science and Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.
authors.forename: Masahito
authors.initials: M
authors.lastname: Taya
mesh_terms: D000818:Animals; D002460:Cell Line; D003432:Cross-Linking Reagents / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D020100:Hydrogels; D051379:Mice
chemical_list: D003432:Cross-Linking Reagents; D020100:Hydrogels
keywords: Alginate; Cell encapsulation; Enzyme; Gelatin; Hydrogel; Surface modification