title: Boron and Poloxamer (F68 and F127) Containing Hydrogel Formulation for Burn Wound Healing.
abstract: Burn injuries, the most common and destructive forms of wounds, are generally accompanied with life-threatening infections, inflammation, reduced angiogenesis, inadequate extracellular matrix production, and lack of growth factor stimulation. In the current study, a new antimicrobial carbopol-based hydrogel formulated with boron and pluronic block copolymers was evaluated for its healing activity using in vitro cell culture techniques and an experimental burn model. Cell viability, gene expression, and wound healing assays showed that gel formulation increased wound healing potential. In vitro tube-like structure formation and histopathological examinations revealed that gel not only increased wound closure by fibroblastic cell activity, but also induced vascularization process. Moreover, gel formulation exerted remarkable antimicrobial effects against bacteria, yeast, and fungi. Migration, angiogenesis, and contraction-related protein expressions including collagen, α-smooth muscle actin, transforming growth factor-β1, vimentin, and vascular endothelial growth factor were considerably enhanced in gel-treated groups. Macrophage-specific antigen showed an oscillating expression at the burn wounds, indicating the role of initial macrophage migration to the wound site and reduced inflammation phase. This is the first study indicating that boron containing hydrogel is able to heal burn wounds effectively. The formulation promoted burn wound healing via complex mechanisms including stimulation of cell migration, growth factor expression, inflammatory response, and vascularization.
authors.affiliation: Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University, Kayisdagi, Istanbul, Turkey.
authors.forename: Selami
authors.initials: S
authors.lastname: Demirci
authors.affiliation: Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University, Kayisdagi, Istanbul, Turkey.
authors.forename: Ayşegül
authors.initials: A
authors.lastname: Doğan
authors.affiliation: Department of Pharmacology and Toxicology, Ataturk University School of Veterinary Medicine, Erzurum, Turkey.
authors.forename: Emre
authors.initials: E
authors.lastname: Karakuş
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Ataturk University, Erzurum, Turkey.
authors.forename: Zekai
authors.initials: Z
authors.lastname: Halıcı
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Ataturk University, Erzurum, Turkey.
authors.forename: Atila
authors.initials: A
authors.lastname: Topçu
authors.affiliation: Department of Pathology, Medical Faculty, Ataturk University, Erzurum, Turkey.
authors.forename: Elif
authors.initials: E
authors.lastname: Demirci
authors.affiliation: Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University, Kayisdagi, Istanbul, Turkey. fsahin@yeditepe.edu.tr.
authors.forename: Fikrettin
authors.initials: F
authors.lastname: Sahin
mesh_terms: D016475:3T3 Cells; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D001896:Boron Compounds / Q000627:therapeutic use*; D002056:Burns / Q000188:drug therapy* / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D015870:Gene Expression / Q000187:drug effects; D020100:Hydrogels / Q000008:administration & dosage / Q000627:therapeutic use; D008297:Male; D051379:Mice; D011092:Polyethylene Glycols / Q000627:therapeutic use*; D011095:Polyethylenes / Q000627:therapeutic use*; D011126:Polypropylenes / Q000627:therapeutic use*; D011409:Propylene Glycols / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000890:Anti-Infective Agents; D001896:Boron Compounds; D020100:Hydrogels; C116176:PEO-PPO-PEO; D011095:Polyethylenes; D011126:Polypropylenes; D011409:Propylene Glycols; D011092:Polyethylene Glycols; C078661:UCON 50-HB-5100
keywords: Boron; Burn; Pluronic; Silver sulfadiazine; Wound healing