title: Sterilization of hydrogen peroxide resistant bacterial spores with stabilized chlorine dioxide.
abstract: Bacillus pumilus SAFR-032 spores isolated from a clean room environment are known to exhibit enhanced resistance to peroxide, desiccation, UV radiation and chemical disinfection than other spore-forming bacteria. The survival of B. pumilus SAFR-032 spores to standard clean room sterilization practices requires development of more stringent disinfection agents. Here, we report the effects of a stabilized chlorine dioxide-based biocidal agent against spores of B. pumilus SAFR-032 and Bacillus subtilis ATCC 6051. Viability was determined via CFU measurement after exposure. Chlorine dioxide demonstrated efficacy towards sterilization of spores of B. pumilus SAFR-032 equivalent or better than exposure to hydrogen peroxide. These results indicate efficacy of chlorine dioxide delivered through a stabilized chlorine dioxide product as a means of sterilization of peroxide- and UV-resistant spores.
authors.affiliation: Department of Chemistry and Biochemistry, Stephenson Life Sciences Research Center, University of Oklahoma, Norman, Oklahoma USA.
authors.forename: Anthony
authors.initials: A
authors.lastname: Friedline
authors.affiliation: Department of Chemistry and Biochemistry, Stephenson Life Sciences Research Center, University of Oklahoma, Norman, Oklahoma USA.
authors.forename: Malcolm
authors.initials: M
authors.lastname: Zachariah
authors.affiliation: Department of Chemistry and Biochemistry, Stephenson Life Sciences Research Center, University of Oklahoma, Norman, Oklahoma USA.
authors.forename: Amy
authors.initials: A
authors.lastname: Middaugh
authors.affiliation: Bio-Cide International, Inc., Norman, Oklahoma USA.
authors.forename: Matt
authors.initials: M
authors.lastname: Heiser
authors.affiliation: Bio-Cide International, Inc., Norman, Oklahoma USA.
authors.forename: Neeraj
authors.initials: N
authors.lastname: Khanna
authors.affiliation: Jet Propulsion Laboratory, California Institute of Technology, Biotechnology and Planetary Protection Group, Pasadena, California USA.
authors.forename: Parag
authors.initials: P
authors.lastname: Vaishampayan
authors.affiliation: Department of Chemistry and Biochemistry, Stephenson Life Sciences Research Center, University of Oklahoma, Norman, Oklahoma USA.
authors.forename: Charles V
authors.initials: CV
authors.lastname: Rice
keywords: Bacillus pumilus; Bacillus subtilis; Chlorine dioxide; Spore killing; Spores