title: Development of Teratocarcinomas and Teratomas in Severely Immunodeficient NOD.Cg-Prkdc(scid) Il2rg(tm1Wjl)/Szj (NSG) Mice.
abstract: The embryonic portion of 7-day-old mouse embryos transplanted to extrauterine sites of syngeneic adult animals gives rise to teratoid tumors, which may be either benign [teratomas (T)] or malignant [teratocarcinomas (TC)]. The incidence of embryo-derived TC varies from one mouse strain to another, indicating that some strains are TC-permissive whereas others are relatively TC-nonpermissive. Embryos of a TC-permissive mouse strain (DBA/2J) and a TC-nonpermissive one (C57BL/6J) were transplanted into NOD.Cg-Prkdc(scid) Il2rg(tm1Wjl)/SzJ (NSG) mice to determine their tumorigenic potential in the absence of functional adaptive and innate immune responses in the hosts. C57BL/6J embryos transplanted to NSG mice gave rise to TC in 31% of cases, whereas the incidence of TC produced from DBA/2J transplanted embryos was 71%. The NSG embryos transplanted to syngeneic hosts gave rise to TC in 67% of cases, allowing the classification of NSG as a TC-permissive strain. A previously reported correlation between teratocarcinoma and splenomegaly was also observed in the NSG mice. The capacity of these tumors to differentiate into the cells and tissues of the normal embryo is mapped through a detailed histological analysis. These data suggest that teratocarcinogenesis, in the absence of host innate and adaptive immunity, is largely determined by the genetic background of the embryo.
authors.affiliation: 1 Epithelial Epigenetics and Development Lab, Institute of Medical Biology , A*STAR, Singapore , Singapore.
authors.forename: Chin Yan
authors.initials: CY
authors.lastname: Lim
authors.affiliation: 1 Epithelial Epigenetics and Development Lab, Institute of Medical Biology , A*STAR, Singapore , Singapore.
authors.forename: Davor
authors.initials: D
authors.lastname: Solter
authors.affiliation: 1 Epithelial Epigenetics and Development Lab, Institute of Medical Biology , A*STAR, Singapore , Singapore.
authors.forename: Barbara B
authors.initials: BB
authors.lastname: Knowles
authors.affiliation: 2 Department of Pathology, The University of Kansas School of Medicine , Kansas City, Kansas.
authors.forename: Ivan
authors.initials: I
authors.lastname: Damjanov
mesh_terms: D000818:Animals; D051747:DNA-Activated Protein Kinase / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D016867:Immunocompromised Host; D053631:Interleukin Receptor Common gamma Subunit / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D016688:Mice, Inbred NOD; D009687:Nuclear Proteins / Q000235:genetics; D018243:Teratocarcinoma / Q000276:immunology / Q000473:pathology*; D013724:Teratoma / Q000276:immunology / Q000473:pathology*
chemical_list: D004268:DNA-Binding Proteins; C507942:Il2rg protein, mouse; D053631:Interleukin Receptor Common gamma Subunit; D009687:Nuclear Proteins; D051747:DNA-Activated Protein Kinase; C497264:Prkdc protein, mouse