title: Polyubiquitin chain-dependent protein degradation in TRIM30 cytoplasmic bodies.
abstract: Viral infection induces numerous tripartite motif (TRIM) proteins to control antiviral immune signaling and viral replication. Particularly, SPRY-containing TRIM proteins are found only in vertebrates and they control target protein degradation by their RING-finger and SPRY domains, and proper cytoplasmic localization. To understand TRIM30 function, we analyzed its localization pattern and putative roles of its RING-finger and SPRY domains. We found that TRIM30 is located in actin-mediated cytoplasmic bodies and produces colocalized ubiquitin chains in SPRY domain- and RING-finger domain-dependent ways that are degraded by autophagy and the proteasome. These results suggest a TRIM protein-dependent degradation mechanism by cytoplasmic body formation with actin networks.
authors.affiliation: Department of Biochemistry, College of Life Science and Technology, Yonsei University, Seoul, Republic of Korea.
authors.forename: Un Yung
authors.initials: UY
authors.lastname: Choi
authors.affiliation: Department of Biochemistry, College of Life Science and Technology, Yonsei University, Seoul, Republic of Korea.
authors.forename: Won Young
authors.initials: WY
authors.lastname: Choi
authors.affiliation: Department of Integrated Omics for Biomedical Science, Yonsei University, Seoul, Republic of Korea.
authors.forename: Ji Yeon
authors.initials: JY
authors.lastname: Hur
authors.affiliation: 1] Department of Biochemistry, College of Life Science and Technology, Yonsei University, Seoul, Republic of Korea [2] Department of Integrated Omics for Biomedical Science, Yonsei University, Seoul, Republic of Korea [3] Center for Cellular and Structural Biology, School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, People's Republic of China.
authors.forename: Young-Joon
authors.initials: YJ
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001343:Autophagy; D002460:Cell Line; D002479:Inclusion Bodies / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D025821:Polyubiquitin / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D054730:Protein Interaction Domains and Motifs; D021381:Protein Transport; D059748:Proteolysis; D054829:RING Finger Domains
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; C528084:TRIM30 alpha protein, mouse; D025821:Polyubiquitin; D046988:Proteasome Endopeptidase Complex