title: Pulsed magnetic stimulation modifies amplitude of action potentials in vitro via ionic channels-dependent mechanism.
abstract: This paper investigates the influence of pulsed magnetic fields (PMFs) on amplitude of evoked, compound action potential (CAP) recorded from the segments of sciatic nerve in vitro. PMFs were applied for 30 min at frequency of 0.16 Hz and intensity of 15 mT. In confirmation of our previous reports, PMF exposure enhanced amplitude of CAPs. The effect persisted beyond PMF activation period. As expected, CAP amplitude was attenuated by antagonists of sodium channel, lidocaine, and tetrodotoxin. Depression of the potential by sodium channels antagonists was reversed by subsequent exposure to PMFs. The effect of elevated potassium concentration and veratridine on the action potential was modified by exposure to PMFs as well. Neither inhibitors of protein kinase C and protein kinase A, nor known free radicals scavengers had any effects on PMF action. Possible mechanisms of PMF action are discussed.
authors.affiliation: Department of Physical Therapy, The Program for Developmental Neuroscience, The College of Staten Island, Staten Island, and Graduate Center/The City University of New York, New York, NY.
authors.forename: Zaghloul
authors.initials: Z
authors.lastname: Ahmed
authors.affiliation: The Department of Biology, The Program for Developmental Neuroscience, The College of Staten Island, Staten Island, and Graduate Center/The City University of New York, New York, NY.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Wieraszko
mesh_terms: D000200:Action Potentials / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology; D008012:Lidocaine / Q000494:pharmacology; D060526:Magnetic Fields; D008297:Male; D051379:Mice; D008839:Microelectrodes; D011188:Potassium / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism; D012584:Sciatic Nerve / Q000187:drug effects / Q000502:physiology; D026941:Sodium Channel Blockers / Q000494:pharmacology; D015222:Sodium Channels / Q000378:metabolism*; D013779:Tetrodotoxin / Q000494:pharmacology; D014701:Veratridine / Q000494:pharmacology; D014810:Vitamin E / Q000378:metabolism
chemical_list: D016166:Free Radical Scavengers; D026941:Sodium Channel Blockers; D015222:Sodium Channels; D014810:Vitamin E; D013779:Tetrodotoxin; D014701:Veratridine; D008012:Lidocaine; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C; D001205:Ascorbic Acid; D011188:Potassium
keywords: axons; potassium channel; pulsed magnetic fields; sodium channel