title: Adult classical glioblastoma with a BRAF V600E mutation.
abstract: The B-Raf proto-oncogene serine/threonine kinase (B-Raf) is a member of the Raf kinase family. The BRAF V600E mutation occurs frequently in certain brain tumors such as pleomorphic xanthoastrocytoma, ganglioglioma, and pilocytic astrocytoma, and less frequently in epithelioid and giant cell glioblastoma. BRAF V600E mutation in these cases has been canonically detected using Sanger sequencing or immunohistochemistry but not with next-generation sequencing (NGS). Moreover, to our knowledge, there is no detailed report of the BRAF V600E mutation in an adult glioblastoma with classical histologic features (c-GBM). Therefore, we performed NGS analysis to determine the mutational status of BRAF of 13 glioblastomas (GBMs) (11 primary and 2 secondary cases) and detected one tumor harboring the BRAF V600E mutation. We report here the detection of the BRAF V600E mutation in a patient with c-GBM and describe the patient's clinical course as well as the results of histopathological analysis.
authors.affiliation: Department of Neurosurgery, Hokuto Hospital, 7-5, Inada, Obihiro, Hokkaido, 080-0039, Japan. yosinobu@hokuto7.or.jp.|Department of Biology and Genetics, Laboratory of Cancer Medical Science, Hokuto Hospital, 7-5, Inada, Obihiro, Hokkaido, 080-0039, Japan. yosinobu@hokuto7.or.jp.
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Takahashi
authors.affiliation: Department of Pathology, Hokuto Hospital, 7-5, Inada, Obihiro, Hokkaido, 080-0039, Japan. pathology2@hokuto7.or.jp.|Department of Biology and Genetics, Laboratory of Cancer Medical Science, Hokuto Hospital, 7-5, Inada, Obihiro, Hokkaido, 080-0039, Japan. pathology2@hokuto7.or.jp.
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Akahane
authors.affiliation: Department of Biology and Genetics, Laboratory of Cancer Medical Science, Hokuto Hospital, 7-5, Inada, Obihiro, Hokkaido, 080-0039, Japan. t-sawada@hokuto7.or.jp.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Sawada
authors.affiliation: Department of Neurosurgery, Hokuto Hospital, 7-5, Inada, Obihiro, Hokkaido, 080-0039, Japan. ikeda@hokuto7.or.jp.
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Ikeda
authors.affiliation: Department of Neurosurgery, Hokuto Hospital, 7-5, Inada, Obihiro, Hokkaido, 080-0039, Japan. tenpaku@hokuto7.or.jp.|Department of Biology and Genetics, Laboratory of Cancer Medical Science, Hokuto Hospital, 7-5, Inada, Obihiro, Hokkaido, 080-0039, Japan. tenpaku@hokuto7.or.jp.
authors.forename: Akira
authors.initials: A
authors.lastname: Tempaku
authors.affiliation: Department of Neurosurgery, Hokuto Hospital, 7-5, Inada, Obihiro, Hokkaido, 080-0039, Japan. yamauchi@hokuto7.or.jp.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Yamauchi
authors.affiliation: Department of Pathology, Hokuto Hospital, 7-5, Inada, Obihiro, Hokkaido, 080-0039, Japan. hnishihara@med.hokudai.ac.jp.|Department of Biology and Genetics, Laboratory of Cancer Medical Science, Hokuto Hospital, 7-5, Inada, Obihiro, Hokkaido, 080-0039, Japan. hnishihara@med.hokudai.ac.jp.|Department of Translational Pathology, Hokkaido University Graduate School of Medicine, N15, W7, Kita-ku, Sapporo, Hokkaido, 060-8638, Japan. hnishihara@med.hokudai.ac.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nishihara
authors.affiliation: Department of Translational Pathology, Hokkaido University Graduate School of Medicine, N15, W7, Kita-ku, Sapporo, Hokkaido, 060-8638, Japan. tanaka@med.hokudai.ac.jp.
authors.forename: Shinya
authors.initials: S
authors.lastname: Tanaka
authors.affiliation: Department of Neurosurgery, Hokuto Hospital, 7-5, Inada, Obihiro, Hokkaido, 080-0039, Japan. nitta@hokuto7.or.jp.
authors.forename: Kazumi
authors.initials: K
authors.lastname: Nitta
authors.affiliation: Department of Neurosurgery, Hokuto Hospital, 7-5, Inada, Obihiro, Hokkaido, 080-0039, Japan. ide@hokuto7.or.jp.
authors.forename: Wataru
authors.initials: W
authors.lastname: Ide
authors.affiliation: Department of Neurosurgery, Hokuto Hospital, 7-5, Inada, Obihiro, Hokkaido, 080-0039, Japan. hashimoto@hokuto7.or.jp.
authors.forename: Ikuo
authors.initials: I
authors.lastname: Hashimoto
authors.affiliation: Department of Neurosurgery, Hokuto Hospital, 7-5, Inada, Obihiro, Hokkaido, 080-0039, Japan. kamada@hokuto7.or.jp.
authors.forename: Hajime
authors.initials: H
authors.lastname: Kamada
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology; D005909:Glioblastoma / Q000235:genetics* / Q000473:pathology; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D009154:Mutation / Q000235:genetics*; D011379:Prognosis; D000090063:Proto-Oncogene Mas; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; C482119:BRAF protein, human; D048493:Proto-Oncogene Proteins B-raf