title: Quantification of nerolidol in mouse plasma using gas chromatography-mass spectrometry.
abstract: Nerolidol is a naturally occurring sesquiterpene found in the essential oils of many types of flowers and plants. It is frequently used in cosmetics, as a food flavoring agent, and in cleaning products. In addition, nerolidol is used as a skin penetration enhancer for transdermal delivery of therapeutic drugs. However, nerolidol is hemolytic at low concentrations. A simple and fast GC-MS method was developed for preliminary quantification and assessment of biological interferences of nerolidol in mouse plasma after oral dosing. Calibration curves were linear in the concentration range of 0.010-5 μg/mL nerolidol in mouse plasma with correlation coefficients (r) greater than 0.99. Limits of detection and quantification were 0.0017 and 0.0035 μg/mL, respectively. The optimized method was successfully applied to the quantification of nerolidol in mouse plasma.
authors.affiliation: Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 1374, São Paulo, SP 05508-000, Brazil.
authors.forename: Alexandre Yukio
authors.initials: AY
authors.lastname: Saito
authors.affiliation: Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 1374, São Paulo, SP 05508-000, Brazil.
authors.forename: Rodrigo Antonio Ceschini
authors.initials: RA
authors.lastname: Sussmann
authors.affiliation: Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 1374, São Paulo, SP 05508-000, Brazil.
authors.forename: Emilia Akemi
authors.initials: EA
authors.lastname: Kimura
authors.affiliation: Department of Biochemistry and Virginia Tech Center for Drug Discovery, M/C 0308, Virginia Tech, Blacksburg, VA 24061, United States.
authors.forename: Maria Belen
authors.initials: MB
authors.lastname: Cassera
authors.affiliation: Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 1374, São Paulo, SP 05508-000, Brazil.
authors.forename: Alejandro Miguel
authors.initials: AM
authors.lastname: Katzin
mesh_terms: D000818:Animals; D002138:Calibration; D003358:Cosmetics / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D057230:Limit of Detection; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009822:Oils, Volatile / Q000737:chemistry; D010949:Plasma / Q000737:chemistry*; D012717:Sesquiterpenes / Q000097:blood* / Q000737:chemistry*
chemical_list: D003358:Cosmetics; D009822:Oils, Volatile; D012717:Sesquiterpenes; C037055:nerolidol
keywords: GC–MS; Nerolidol; Plasma; Quantification; Sesquiterpene