title: Fundus-controlled two-color dark adaptometry with the Microperimeter MP1.
abstract: OBJECTIVE
  The aim of this study was to provide fundus-controlled two-color adaptometry with an existing device. A quick and easy approach extends the application possibilities of a commercial fundus-controlled perimeter.
  
  
  METHODS
  An external filter holder was placed in front the objective lens of the MP1 (Nidek, Italy) and fitted with filters to modify background, stimulus intensity, and color. Prior to dark adaptometry, the subject's visual sensitivity profile was measured for red and blue stimuli to determine whether rods or cones or both mediated the absolute threshold. After light adaptation, 20 healthy subjects were investigated with a pattern covering six spots at the posterior pole of the retina up to 45 min of dark adaptation. Thresholds were determined using a 200 ms red Goldmann IV and a blue Goldmann II stimulus.
  
  
  RESULTS
  The pre-test sensitivity showed a typical distribution of values along the meridian, with high peripheral light increment sensitivity (LIS) and low central LIS for rods and the reverse for cones. After bleach, threshold recovery had a classic biphasic shape. The absolute threshold was reached after approximately 10 min for the red and 15 min for the blue stimulus.
  
  
  CONCLUSIONS
  Two-color fundus-controlled adaptometry with a commercial MP1 without internal changes to the device provides a quick and easy examination of rod and cone function during dark adaptation at defined retinal loci of the posterior pole. This innovative method will be helpful to measure rod vs. cone function at known loci of the posterior pole in early stages of retinal degenerations.
authors.affiliation: Department of Ophthalmology, Justus-Liebig-University, Friedrichstrasse 18, 35392, Giessen, Germany, Wadim.Bowl@augen.med.uni-giessen.de.
authors.forename: Wadim
authors.initials: W
authors.lastname: Bowl
authors.forename: Knut
authors.initials: K
authors.lastname: Stieger
authors.forename: Birgit
authors.initials: B
authors.lastname: Lorenz
mesh_terms: D000293:Adolescent; D000328:Adult; D003118:Color Perception / Q000502:physiology*; D003623:Dark Adaptation / Q000502:physiology*; D005260:Female; D005654:Fundus Oculi; D006801:Humans; D008297:Male; D008875:Middle Aged; D020419:Photoreceptor Cells, Vertebrate / Q000502:physiology*; D012684:Sensory Thresholds; D058609:Visual Field Tests / Q000295:instrumentation* / Q000379:methods; D014794:Visual Fields / Q000502:physiology; D055815:Young Adult