title: Viability and functional assessment of murine pancreatic islets after transportation between Korea and Japan.
abstract: BACKGROUND
  Organ donor scarcity remains a restricting factor for pancreatic islet transplantation. To date, limited information is available on the impact of long-distance transportation on transplantable pancreatic islets. The objective of this study was to assess the effects of transportation on the viability and function of murine pancreatic islet cells.
  
  
  METHODS
  The isolated murine pancreatic islets were transported from Japan to Korea with the use of commercial modes of transportation: subway and commercial airplane. After transportation, the islets were assessed by performing a viability assay and by evaluating the islets' insulin secretion in response to glucose stimulation. A comparative study was performed for evaluating the insulin secretory responses of transported and control islets (not transported).
  
  
  RESULTS
  There was no evidence of contamination in the transported pancreatic islets. No significant differences were observed in the viability and functionality of the transported and control islet cells.
  
  
  CONCLUSIONS
  These findings show the feasibility of pancreatic islet transportation from Japan to Korea. Our data could be used not only for the inter-Asian but also for global advancement of animal and human islet transportation methods and transplantation research.
authors.affiliation: Regenerative Medicine Laboratory, Center for Stem Cell Research, Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology, Konkuk University, Seoul, Republic of Korea.
authors.forename: S
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Regenerative Medicine, Yokohama City University Graduate School of Medicine, Yokohama, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Takahashi
authors.affiliation: Regenerative Medicine Laboratory, Center for Stem Cell Research, Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology, Konkuk University, Seoul, Republic of Korea.
authors.forename: K M
authors.initials: KM
authors.lastname: Lee
authors.affiliation: Department of Regenerative Medicine, Yokohama City University Graduate School of Medicine, Yokohama, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Mizuno
authors.affiliation: Regenerative Medicine Laboratory, Center for Stem Cell Research, Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology, Konkuk University, Seoul, Republic of Korea.
authors.forename: J G
authors.initials: JG
authors.lastname: Nemeno
authors.affiliation: Department of Regenerative Medicine, Yokohama City University Graduate School of Medicine, Yokohama, Japan; Project Leader of Advanced Medical Research Center, Yokohama City University, Yokohama, Japan; PRESTO, Japan Science and Technology Agency, Saitama, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Takebe
authors.affiliation: Regenerative Medicine Laboratory, Center for Stem Cell Research, Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology, Konkuk University, Seoul, Republic of Korea; Department of Veterinary Medicine, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea. Electronic address: jeongik@konkuk.ac.kr.
authors.forename: J I
authors.initials: JI
authors.lastname: Lee
mesh_terms: D000818:Animals; D005240:Feasibility Studies; D006801:Humans; D007391:International Cooperation; D007515:Islets of Langerhans / Q000502:physiology*; D016381:Islets of Langerhans Transplantation; D007564:Japan; D051379:Mice; D008810:Mice, Inbred C57BL; D056910:Republic of Korea; D009927:Tissue and Organ Procurement / Q000379:methods*; D014186:Transportation