title: EGFR/ERBB receptors differentially modulate sebaceous lipogenesis.
abstract: The roles of the epidermal growth factor receptor (EGFR) in sebaceous glands remain poorly explored. We show that human sebocytes express EGFR and lower levels of ERBB2 and ERBB3, all receptors being downregulated after the induction of lipid synthesis. Nile red staining showed that siRNA-mediated downregulation of EGFR or ERBB3 increases lipid accumulation, whereas ERBB2 downregulation has no effect. Spectrometry confirmed induction of triglycerides after EGFR or ERBB3 downregulation and revealed induction of cholesteryl esters after downregulation of EGFR, ERBB2 or ERBB3. Thus, EGFR/ERBB receptors differentially modulate sebaceous lipogenesis, a key feature of sebaceous gland physiology and of several skin diseases.
authors.affiliation: Institute of Molecular Animal Breeding and Biotechnology, Gene Center, LMU Munich, Munich, Germany.
authors.forename: Maik
authors.initials: M
authors.lastname: Dahlhoff
authors.affiliation: Laboratory of Cutaneous Physiopathology and Integrated Center of Metabolomics Research, San Gallicano Dermatologic Institute, IRCCS, Rome, Italy.
authors.forename: Emanuela
authors.initials: E
authors.lastname: Camera
authors.affiliation: Laboratory of Cutaneous Physiopathology and Integrated Center of Metabolomics Research, San Gallicano Dermatologic Institute, IRCCS, Rome, Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Ludovici
authors.affiliation: Laboratory of Cutaneous Physiopathology and Integrated Center of Metabolomics Research, San Gallicano Dermatologic Institute, IRCCS, Rome, Italy.
authors.forename: Mauro
authors.initials: M
authors.lastname: Picardo
authors.affiliation: Human Biology and BioImaging, Department of Biology II, LMU Munich, Germany.
authors.forename: Udo
authors.initials: U
authors.lastname: Müller
authors.affiliation: Human Biology and BioImaging, Department of Biology II, LMU Munich, Germany.
authors.forename: Heinrich
authors.initials: H
authors.lastname: Leonhardt
authors.affiliation: Departments of Dermatology, Venereology, Allergology and Immunology, Dessau Medical Center, Dessau, Germany.
authors.forename: Christos C
authors.initials: CC
authors.lastname: Zouboulis
authors.affiliation: Institute of Molecular Animal Breeding and Biotechnology, Gene Center, LMU Munich, Munich, Germany. Electronic address: marlon.schneider@lmu.de.
authors.forename: Marlon R
authors.initials: MR
authors.lastname: Schneider
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D002460:Cell Line; D002788:Cholesterol Esters / Q000378:metabolism; D015536:Down-Regulation; D066246:ErbB Receptors / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D008024:Ligands; D019787:Linoleic Acid / Q000378:metabolism; D050155:Lipogenesis; D034622:RNA Interference; D034741:RNA, Small Interfering; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D020893:Receptor, ErbB-3 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D012627:Sebaceous Glands / Q000201:enzymology / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D015415:Biomarkers; D002788:Cholesterol Esters; D066246:ErbB Receptors; D005230:Fatty Acids, Nonesterified; D008024:Ligands; D019787:Linoleic Acid; D034741:RNA, Small Interfering; D018719:Erb-b2 Receptor Tyrosine Kinases; D020893:Receptor, ErbB-3; D014280:Triglycerides; C512478:EGFR protein, human; C508053:ERBB2 protein, human; C581292:ERBB3 protein, human
keywords: ERBB; Epidermal growth factor receptor; Sebaceous gland; Sebaceous lipogenesis