title: Activation of Wnt/β-catenin signalling promotes mesenchymal stem cells to repair injured alveolar epithelium induced by lipopolysaccharide in mice.
abstract: Mesenchymal stem cells (MSCs) have potential for re-epithelization and recovery in acute respiratory distress syndrome (ARDS). In a previous in vitro study, the results showed that the canonical Wnt/β-catenin pathway promoted the differentiation of MSCs into type II alveolar epithelial cells, conferred resistance to oxidative stress, and promoted their migration, suggesting that the Wnt/β-catenin pathway might be one of the key mechanisms underling the therapeutic effect of mouse MSCs in ARDS.
  
  
  
  Mouse MSCs stable transfected with β-catenin or green fluorescent protein control were transplanted intratracheally into the ARDS mice induced by lipopolysaccharide. Lung tissue injury and repair assessment were examined using haematoxylin and eosin staining, lung injury scoring, Masson's trichrome staining and fibrosis scoring. Homing and differentiation of mouse MSCs were assayed by labelling and tracing MSCs using NIR815 dye, immunofluorescent staining, and Western immunoblot analysis. The inflammation and permeability were evaluated by detecting the cytokine and protein measurements in bronchoalveolar lavage fluid using enzyme-linked immunosorbent assay.
  
  
  
  In this study, β-catenin-overexpressing MSC engraftment led to more significant effects than the GFP controls, including the retention of the MSCs in the lung, differentiation into type II alveolar epithelial cells, improvement in alveolar epithelial permeability, and the pathologic impairment of the lung tissue.
  
  
  
  These results suggest that the activation of canonical Wnt/β-catenin pathway by mouse MSCs by overexpressing β-catenin could further improve the protection of mouse MSCs against epithelial impair and the therapeutic effects of mouse MSCs in ARDS mice.
authors.affiliation: Department of Critical Care Medicine, Zhong-da Hospital, School of Medicine, Southeast University, 87 Dingjiaqiao Road, Nanjing, 210009, People's Republic of China. cccsssxxx1980@163.com.|Department of Critical Care Medicine, The Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao, 266003, People's Republic of China. cccsssxxx1980@163.com.
authors.forename: Shi-xia
authors.initials: SX
authors.lastname: Cai
authors.affiliation: Department of Critical Care Medicine, Zhong-da Hospital, School of Medicine, Southeast University, 87 Dingjiaqiao Road, Nanjing, 210009, People's Republic of China. airanliu2013@gmail.com.
authors.forename: Ai-ran
authors.initials: AR
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing, 210009, People's Republic of China. chensong07@hotmail.com.
authors.forename: Song
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Critical Care Medicine, Zhong-da Hospital, School of Medicine, Southeast University, 87 Dingjiaqiao Road, Nanjing, 210009, People's Republic of China. hhl0408@163.com.
authors.forename: Hong-li
authors.initials: HL
authors.lastname: He
authors.affiliation: Department of Critical Care Medicine, Zhong-da Hospital, School of Medicine, Southeast University, 87 Dingjiaqiao Road, Nanjing, 210009, People's Republic of China. chenqihong00@163.com.
authors.forename: Qi-hong
authors.initials: QH
authors.lastname: Chen
authors.affiliation: Department of Critical Care Medicine, Zhong-da Hospital, School of Medicine, Southeast University, 87 Dingjiaqiao Road, Nanjing, 210009, People's Republic of China. xujingyuanmail@163.com.
authors.forename: Jing-yuan
authors.initials: JY
authors.lastname: Xu
authors.affiliation: Department of Critical Care Medicine, Zhong-da Hospital, School of Medicine, Southeast University, 87 Dingjiaqiao Road, Nanjing, 210009, People's Republic of China. panchun1982@gmail.com.
authors.forename: Chun
authors.initials: C
authors.lastname: Pan
authors.affiliation: Department of Critical Care Medicine, Zhong-da Hospital, School of Medicine, Southeast University, 87 Dingjiaqiao Road, Nanjing, 210009, People's Republic of China. yiyiyang2004@gmail.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Critical Care Medicine, Zhong-da Hospital, School of Medicine, Southeast University, 87 Dingjiaqiao Road, Nanjing, 210009, People's Republic of China. fmguo2003@139.com.
authors.forename: Feng-mei
authors.initials: FM
authors.lastname: Guo
authors.affiliation: Department of Critical Care Medicine, Zhong-da Hospital, School of Medicine, Southeast University, 87 Dingjiaqiao Road, Nanjing, 210009, People's Republic of China. yz_huang@126.com.
authors.forename: Ying-zi
authors.initials: YZ
authors.lastname: Huang
authors.affiliation: Department of Critical Care Medicine, Zhong-da Hospital, School of Medicine, Southeast University, 87 Dingjiaqiao Road, Nanjing, 210009, People's Republic of China. liulingdoctor@gmail.com.
authors.forename: Ling
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Critical Care Medicine, Zhong-da Hospital, School of Medicine, Southeast University, 87 Dingjiaqiao Road, Nanjing, 210009, People's Republic of China. haiboq2000@gmail.com.
authors.forename: Hai-bo
authors.initials: HB
authors.lastname: Qiu
mesh_terms: D055371:Acute Lung Injury / Q000628:therapy*; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002465:Cell Movement; D064987:Cell- and Tissue-Based Therapy; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology*; D008070:Lipopolysaccharides; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress / Q000502:physiology; D011650:Pulmonary Alveoli / Q000378:metabolism* / Q000473:pathology; D012128:Respiratory Distress Syndrome / Q000628:therapy*; D020545:Respiratory Mucosa / Q000166:cytology*; D051153:Wnt Proteins / Q000378:metabolism; D060449:Wnt Signaling Pathway / Q000502:physiology*; D051176:beta Catenin / Q000378:metabolism
chemical_list: D008070:Lipopolysaccharides; D051153:Wnt Proteins; D051176:beta Catenin