title: Single-cell, locus-specific bisulfite sequencing (SLBS) for direct detection of epimutations in DNA methylation patterns.
abstract: Stochastic epigenetic changes drive biological processes, such as development, aging and disease. Yet, epigenetic information is typically collected from millions of cells, thereby precluding a more precise understanding of cell-to-cell variability and the pathogenic history of epimutations. Here we present a novel procedure for directly detecting epimutations in DNA methylation patterns using single-cell, locus-specific bisulfite sequencing (SLBS). We show that within gene promoter regions of mouse hepatocytes the epimutation rate is two orders of magnitude higher than the mutation rate.
authors.affiliation: Department of Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA silvia.gravina@einstein.yu.edu.
authors.forename: Silvia
authors.initials: S
authors.lastname: Gravina
authors.affiliation: Department of Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
authors.forename: Shireen
authors.initials: S
authors.lastname: Ganapathi
authors.affiliation: Department of Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA silvia.gravina@einstein.yu.edu.
authors.forename: Jan
authors.initials: J
authors.lastname: Vijg
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D056426:Genetic Loci; D051379:Mice; D008810:Mice, Inbred C57BL; D059645:Mutation Rate; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA / Q000379:methods*; D059010:Single-Cell Analysis; D013447:Sulfites
chemical_list: D013447:Sulfites; C042345:hydrogen sulfite