title: [Three dimensional structure of the dimeric gene-engineered variant of green fluorescent protein EGFP-K162Q in P6(1) crystal space group].
abstract: The crystal structure of the dimeric green fluorescent protein EGFP-K162Q with C-terminal deletion MDELYK (EGFPv) has been determined in space group P6 at resolution 1.34 A. The obtained structure has been compared with that of the monomeric form of EGFP (green biomarker with enhanced photophysical properties) determined in other crystal space group P2(1)2(1)2(1) at resolution 1.50 and 1.35 A [1, 2]. Two subunits in the EGFPv structure are packed at 75 degrees with the contact surface approximately 800 A2. The dimeric structure is stabilized by six hydrogen bonds and the central hydrophobic core built of six residues. The RMSD value for Calpha atoms of 3-230 residues in the superimposed P61 and P2(1)2(1)2(1) structures is 0.55 A. The distinguishing feature of EGFPv- P6(1) structure, compared with that of EGFP-P2(1)2(1)2(1), is the noticeable difference in orientation of the Glu222 side chain and also new conformation of the loop fragment 155-159 with deviations among the Calpha atoms of superimposed structures reaching for Lys156 - 4.6 A and Lys158 - 5.5 A
authors.forename: N V
authors.initials: NV
authors.lastname: Pletneva
authors.forename: S V
authors.initials: SV
authors.lastname: Pletnev
authors.forename: A M
authors.initials: AM
authors.lastname: Bogdanov
authors.forename: E A
authors.initials: EA
authors.lastname: Goriacheva
authors.forename: I V
authors.initials: IV
authors.lastname: Artem'ev
authors.forename: E A
authors.initials: EA
authors.lastname: Suslova
authors.forename: S F
authors.initials: SF
authors.lastname: Arkhipova
authors.forename: V Z
authors.initials: VZ
authors.lastname: Pletnev
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics*; D001665:Binding Sites; D018360:Crystallography, X-Ray; D049452:Green Fluorescent Proteins / Q000737:chemistry* / Q000235:genetics; D006860:Hydrogen Bonding; D008958:Models, Molecular; D011485:Protein Binding; D011487:Protein Conformation; D055503:Protein Multimerization; D017433:Protein Structure, Secondary
chemical_list: C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins