title: ABA inducible rice protein phosphatase 2C confers ABA insensitivity and abiotic stress tolerance in Arabidopsis.
abstract: Arabidopsis PP2C belonging to group A have been extensively worked out and known to negatively regulate ABA signaling. However, rice (Oryza sativa) orthologs of Arabidopsis group A PP2C are scarcely characterized functionally. We have identified a group A PP2C from rice (OsPP108), which is highly inducible under ABA, salt and drought stresses and localized predominantly in the nucleus. Genetic analysis revealed that Arabidopsis plants overexpressing OsPP108 are highly insensitive to ABA and tolerant to high salt and mannitol stresses during seed germination, root growth and overall seedling growth. At adult stage, OsPP108 overexpression leads to high tolerance to salt, mannitol and drought stresses with far better physiological parameters such as water loss, fresh weight, chlorophyll content and photosynthetic potential (Fv/Fm) in transgenic Arabidopsis plants. Expression profile of various stress marker genes in OsPP108 overexpressing plants revealed interplay of ABA dependent and independent pathway for abiotic stress tolerance. Overall, this study has identified a potential rice group A PP2C, which regulates ABA signaling negatively and abiotic stress signaling positively. Transgenic rice plants overexpressing this gene might provide an answer to the problem of low crop yield and productivity during adverse environmental conditions.
authors.affiliation: Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, Dhaula Kuan, New Delhi, India.
authors.forename: Amarjeet
authors.initials: A
authors.lastname: Singh
authors.affiliation: Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, Dhaula Kuan, New Delhi, India.
authors.forename: Saroj K
authors.initials: SK
authors.lastname: Jha
authors.affiliation: Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, Dhaula Kuan, New Delhi, India.
authors.forename: Jayram
authors.initials: J
authors.lastname: Bagri
authors.affiliation: Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, Dhaula Kuan, New Delhi, India.
authors.forename: Girdhar K
authors.initials: GK
authors.lastname: Pandey
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism / Q000494:pharmacology*; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D018525:Germination; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics* / Q000502:physiology; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D054046:Plant Stomata / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D017386:Sequence Homology, Amino Acid; D013312:Stress, Physiological; D014026:Nicotiana / Q000235:genetics
chemical_list: D010940:Plant Proteins; D000040:Abscisic Acid; D010749:Phosphoprotein Phosphatases