title: Burkholderia metalliresistens sp. nov., a multiple metal-resistant and phosphate-solubilising species isolated from heavy metal-polluted soil in Southeast China.
abstract: A metal-resistant and phosphate-solubilising bacterium, designated as strain D414(T), was isolated from heavy metal (Pb, Cd, Cu and Zn)-polluted paddy soils at the surrounding area of Dabao Mountain Mine in Southeast China. The minimum inhibitory concentrations of heavy metals for strain D414(T) were 2000 mg L(-1) (Cd), 800 mg L(-1) (Pb), 150 mg L(-1) (Cu) and 2500 mg L(-1) (Zn). The strain possessed plant growth-promoting properties, such as 1-aminocyclopropane-1-carboxylate assimilation, indole production and phosphate solubilisation. Analysis of 16S rRNA gene sequence indicated that the isolate is a member of the genus Burkholderia where strain D414(T) formed a distinct phyletic line with validly described Burkholderia species. Strain D414(T) is closely related to Burkholderia tropica DSM 15359(T), B. bannensis NBRC E25(T) and B. unamae DSM 17197(T), with 98.5, 98.3 and 98.3 % sequence similarities, respectively. Furthermore, less than 34 % DNA-DNA relatedness was detected between strain D414(T) and the type strains of the phylogenetically closest species of Burkholderia. The dominant fatty acids of strain D414(T) were C14:0, C16:0, C17:0 cyclo and C18:1 ω7c. The DNA G+C content was 62.3 ± 0.5 mol%. On the basis of genotypic, phenotypic and phylogenetic data, strain D414(T) represents a novel species, for which the name Burkholderia metalliresistens sp. nov. is proposed, with D414(T) (=CICC 10561(T) = DSM 26823(T)) as the type strain.
authors.affiliation: Innovation Team of Remediation for Heavy Metal Contaminated Farmland of Chinese Academic of Agricultural Sciences, Institute of Agro-Environmental Protection, Ministry of Agriculture, Tianjin, 300191, People's Republic of China, frankerry@163.com.
authors.forename: Jun Kang
authors.initials: JK
authors.lastname: Guo
authors.forename: Yong Zhen
authors.initials: YZ
authors.lastname: Ding
authors.forename: Ren Wei
authors.initials: RW
authors.lastname: Feng
authors.forename: Rui Gang
authors.initials: RG
authors.lastname: Wang
authors.forename: Ying Ming
authors.initials: YM
authors.lastname: Xu
authors.forename: Chun
authors.initials: C
authors.lastname: Chen
authors.forename: Xiu Li
authors.initials: XL
authors.lastname: Wei
authors.forename: Wei Min
authors.initials: WM
authors.lastname: Chen
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D019117:Burkholderia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D002681:China; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D024881:Drug Resistance, Bacterial; D005227:Fatty Acids / Q000032:analysis; D007211:Indoles / Q000378:metabolism; D019216:Metals, Heavy / Q000633:toxicity*; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010710:Phosphates / Q000378:metabolism*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000633:toxicity
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D007211:Indoles; D019216:Metals, Heavy; D010710:Phosphates; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; C030374:indole