title: Synthesis and application of glycoconjugate-functionalized magnetic nanoparticles as potent anti-adhesion agents for reducing enterotoxigenic Escherichia coli infections.
abstract: Polyethylene oxide stabilized magnetic nanoparticles (PEO-MNPs) bio-functionalized with glycoconjugate (Neu5Ac(α2-3)Gal(β1-4)Glcβ-sp) (GM3-MNPs) are synthesized using click chemistry. Interaction of GM3-MNPs with Enterotoxigenic Escherichia coli (ETEC) strain K99 (EC K99) is investigated using different microscopic techniques. Our results suggest that GM3-MNPs can effectively act as non-antibiotic anti-adhesion agents for treating ETEC infections.
authors.affiliation: Department of Biological Sciences, Clemson University, Clemson, SC 29634, USA. tzuenrt@clemson.edu.
authors.forename: Yash S
authors.initials: YS
authors.lastname: Raval
authors.forename: Roland
authors.initials: R
authors.lastname: Stone
authors.forename: Benjamin
authors.initials: B
authors.lastname: Fellows
authors.forename: Bin
authors.initials: B
authors.lastname: Qi
authors.forename: Guohui
authors.initials: G
authors.lastname: Huang
authors.forename: O Thompson
authors.initials: OT
authors.lastname: Mefford
authors.forename: Tzuen-Rong J
authors.initials: TR
authors.lastname: Tzeng
mesh_terms: D001422:Bacterial Adhesion / Q000187:drug effects* / Q000502:physiology; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000494:pharmacology*; D054307:Enterotoxigenic Escherichia coli / Q000187:drug effects* / Q000502:physiology; D004927:Escherichia coli Infections / Q000382:microbiology / Q000517:prevention & control; D006001:Glycoconjugates / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D058185:Magnetite Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry*; D058726:Nanoconjugates / Q000008:administration & dosage / Q000737:chemistry
chemical_list: D020099:Coated Materials, Biocompatible; D006001:Glycoconjugates; D058185:Magnetite Nanoparticles; D058726:Nanoconjugates