title: DNA damage response and spindle assembly checkpoint function throughout the cell cycle to ensure genomic integrity.
abstract: Errors in replication or segregation lead to DNA damage, mutations, and aneuploidies. Consequently, cells monitor these events and delay progression through the cell cycle so repair precedes division. The DNA damage response (DDR), which monitors DNA integrity, and the spindle assembly checkpoint (SAC), which responds to defects in spindle attachment/tension during metaphase of mitosis and meiosis, are critical for preventing genome instability. Here we show that the DDR and SAC function together throughout the cell cycle to ensure genome integrity in C. elegans germ cells. Metaphase defects result in enrichment of SAC and DDR components to chromatin, and both SAC and DDR are required for metaphase delays. During persistent metaphase arrest following establishment of bi-oriented chromosomes, stability of the metaphase plate is compromised in the absence of DDR kinases ATR or CHK1 or SAC components, MAD1/MAD2, suggesting SAC functions in metaphase beyond its interactions with APC activator CDC20. In response to DNA damage, MAD2 and the histone variant CENPA become enriched at the nuclear periphery in a DDR-dependent manner. Further, depletion of either MAD1 or CENPA results in loss of peripherally associated damaged DNA. In contrast to a SAC-insensitive CDC20 mutant, germ cells deficient for SAC or CENPA cannot efficiently repair DNA damage, suggesting that SAC mediates DNA repair through CENPA interactions with the nuclear periphery. We also show that replication perturbations result in relocalization of MAD1/MAD2 in human cells, suggesting that the role of SAC in DNA repair is conserved.
authors.affiliation: Department of Molecular and Cellular Biology; Biochemistry, Molecular Cellular and Developmental Biology Graduate Group, University of California, Davis, Davis, California, United States of America.
authors.forename: Katherine S
authors.initials: KS
authors.lastname: Lawrence
authors.affiliation: Department of Molecular and Cellular Biology; Biochemistry, Molecular Cellular and Developmental Biology Graduate Group, University of California, Davis, Davis, California, United States of America.
authors.forename: Thinh
authors.initials: T
authors.lastname: Chau
authors.affiliation: Department of Molecular and Cellular Biology; Biochemistry, Molecular Cellular and Developmental Biology Graduate Group, University of California, Davis, Davis, California, United States of America.
authors.forename: JoAnne
authors.initials: J
authors.lastname: Engebrecht
mesh_terms: D000818:Animals; D001324:Autoantigens / Q000235:genetics; D017173:Caenorhabditis elegans / Q000235:genetics; D000076247:Centromere Protein A; D002843:Chromatin / Q000235:genetics; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics; D002875:Chromosomes / Q000235:genetics; D004249:DNA Damage / Q000235:genetics*; D004260:DNA Repair / Q000235:genetics; D042822:Genomic Instability / Q000235:genetics*; D006801:Humans; D059566:M Phase Cell Cycle Checkpoints / Q000235:genetics*; D064246:Mad2 Proteins / Q000235:genetics*; D008677:Metaphase / Q000235:genetics
chemical_list: D001324:Autoantigens; C000619700:CENPA protein, human; D000076247:Centromere Protein A; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; C577697:MAD2L1 protein, human; D064246:Mad2 Proteins