title: Effect of the pH in the formation of β-galactosidase microparticles produced by a spray-drying process.
abstract: The objective of this work was to investigate the influence of pH in the microencapsulation process, using a modified chitosan to microencapsulate the enzyme β-galactosidase, by a spray-drying technique. Structural analysis of the surface of the particles was performed by scanning electron microscopy (SEM), showing that the obtained microparticles have an average diameter smaller than 3.5 μm and in general a regular shape. The activity of the enzyme was studied by spectrophotometric methods using the substrate O-nitrophenyl-β,D-galactopyranoside (ONPG). The parameters of Michaelis-Menten were calculated. The value of Km decreases with the decrease of the pH, which can be associated to an increase of the affinity between the enzyme and substrate to smaller pH's. The highest value of the parameter Vmax, representing the maximum reaction rate at a given enzyme concentration, was obtained at pH 6.
authors.affiliation: LEPABE, Departamento de Engenharia Química, Faculdade de Engenharia da Universidade do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal. Electronic address: berta@fe.up.pt.
authors.forename: Berta N
authors.initials: BN
authors.lastname: Estevinho
authors.affiliation: LEPABE, Departamento de Engenharia Química, Faculdade de Engenharia da Universidade do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal; Departamento de Engenharia Química, Universidade Federal Rural do Rio de Janeiro, Brazil.
authors.forename: Irena
authors.initials: I
authors.lastname: Ramos
authors.affiliation: LEPABE, Departamento de Engenharia Química, Faculdade de Engenharia da Universidade do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal.
authors.forename: Fernando
authors.initials: F
authors.lastname: Rocha
mesh_terms: D048271:Chitosan; D004339:Drug Compounding / Q000379:methods; D004789:Enzyme Activation; D006863:Hydrogen-Ion Concentration; D008863:Microspheres; D010316:Particle Size; D001616:beta-Galactosidase
chemical_list: D048271:Chitosan; D001616:beta-Galactosidase
keywords: Microencapsulation; Modified chitosan; Spray-drying; β-Galactosidase