title: [Effects of steaming and baking on content of alkaloids in Aconite Lateralis Radix (Fuzi)].
abstract: To study the effect of steaming and baking process on contents of alkaloids in Aconite Lateralis Radix (Fuzi), 13 alkaloids were analyzed by UPLC-MS/MS equipped with ESI ion source in MRM mode. In steaming process, the contents of diester-diterpenoid alkaloids decreased rapidly, the contents of monoester-diterpenoid alkaloids firstly increased, reached the peak at 40 min, and then deceased gradually. The contents of aconine alkaloids (mesaconine, aconine and hypaconine) increased all the time during processing, while the contents of fuziline, songorine, karacoline, salsolionl were stable or slightly decreased. In baking process, dynamic variations of alkaloids were different from that in the steaming process. Diester-diterpenoid alkaloids were degraded slightly slower than in steaming process. Monoester-diterpenoid alkaloids, aconine alkaloids and the total alkaloids had been destroyed at different degrees, their contents were significantly lower than the ones in steaming Fuzi at the same processing time. This experiment revealed the dynamic variations of alkaloids in the course of steaming and baking. Two processing methods which can both effectively remove the toxic ingredients and retain the active ingredients are simple and controllable, and are valuable for popularization and application.
authors.forename: Chang-lin
authors.initials: CL
authors.lastname: Yang
authors.forename: Zhi-fang
authors.initials: ZF
authors.lastname: Huang
authors.forename: Yi-han
authors.initials: YH
authors.lastname: Zhang
authors.forename: Yu-hong
authors.initials: YH
authors.lastname: Liu
authors.forename: Yun-huan
authors.initials: YH
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Jin-hai
authors.initials: JH
authors.lastname: Yi
mesh_terms: D000157:Aconitine / Q000031:analogs & derivatives / Q000302:isolation & purification; D031841:Aconitum / Q000737:chemistry*; D000470:Alkaloids / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification*; D006358:Hot Temperature; D010936:Plant Extracts / Q000302:isolation & purification*; D013227:Steam; D053719:Tandem Mass Spectrometry; D013997:Time Factors
chemical_list: D000470:Alkaloids; D004224:Diterpenes; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D013227:Steam; C575009:fuzi drug herbal; C047306:aconine; D000157:Aconitine