title: Comparative sequence analyses of La Crosse virus strain isolated from patient with fatal encephalitis, Tennessee, USA.
abstract: We characterized a La Crosse virus (LACV) isolate from the brain of a child who died of encephalitis-associated complications in eastern Tennessee, USA, during summer 2012. We compared the isolate with LACV sequences from mosquitoes collected near the child's home just after his postmortem diagnosis. In addition, we conducted phylogenetic analyses of these and other sequences derived from LACV strains representing varied temporal, geographic, and ecologic origins. Consistent with historical findings, results of these analyses indicate that a limited range of LACV lineage I genotypes is associated with severe clinical outcomes.
authors.forename: Amy J
authors.initials: AJ
authors.lastname: Lambert
authors.forename: Rebecca Trout
authors.initials: RT
authors.lastname: Fryxell
authors.forename: Kimberly
authors.initials: K
authors.lastname: Freyman
authors.forename: Armando
authors.initials: A
authors.lastname: Ulloa
authors.forename: Jason O
authors.initials: JO
authors.lastname: Velez
authors.forename: Dave
authors.initials: D
authors.lastname: Paulsen
authors.forename: Robert S
authors.initials: RS
authors.lastname: Lanciotti
authors.forename: Abelardo
authors.initials: A
authors.lastname: Moncayo
mesh_terms: D000818:Animals; D002648:Child; D004670:Encephalitis, California / Q000175:diagnosis / Q000453:epidemiology* / Q000635:transmission / Q000821:virology*; D017809:Fatal Outcome; D016679:Genome, Viral; D006801:Humans; D018062:La Crosse virus / Q000145:classification* / Q000235:genetics*; D008297:Male; D010802:Phylogeny; D012367:RNA, Viral; D012703:Serotyping; D013714:Tennessee / Q000453:epidemiology
chemical_list: D012367:RNA, Viral
keywords: La Crosse virus; Tennessee; United States; children; encephalitis; mosquitoes; sequence; vector-borne infections; viruses