title: Study of prognostic significance of marrow angiogenesis assessment in patients with de novo acute leukemia.
abstract: BACKGROUND
  Angiogenesis is the highly ordered formation of new blood vessels from pre-existing vessels. It is seen throughout growth, in wound healing, menses, and is important in cancer, where pro- and antiangiogenic signals can be released by cancer cells, endothelial cells, stromal cells, blood, and the extracellular matrix. Aim of the study is to use standardized method for counting blood vessels to verify the significance and prognostic value of assessing marrow angiogenesis at diagnosis of de novo acute leukemia.
  
  
  METHODS
  The study included 70 newly diagnosed acute leukemia cases and a control group composed of 35 bone marrow biopsy sections obtained from breast cancer patients. Examination of CD34 immunohistochemically stained sections for the assessment of marrow angiogenesis by quantification of its microvessel density (MVD).
  
  
  RESULTS
  MVD was significantly increased in acute leukemia patients in comparison to control group (P-value <0.001). Increased MVD was associated with unfavorable outcome.
  
  
  CONCLUSIONS
  The study demonstrated an evidence of increased angiogenesis in acute leukemia detected by high bone marrow MVD which may play a significant role in leukemic process. Understanding its role may help in designing new therapeutic strategies for acute leukemia.
authors.forename: Ahmed
authors.initials: A
authors.lastname: AbdElAal Asmaa
authors.forename: Reham Abdel Aleem
authors.initials: RA
authors.lastname: Afify
authors.forename: Amr ElSayed
authors.initials: AE
authors.lastname: Zaher
authors.forename: Mosaad Mahmoud
authors.initials: MM
authors.lastname: ElGammal
authors.forename: Asmaa M
authors.initials: AM
authors.lastname: Atef
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000970:Antineoplastic Agents / Q000627:therapeutic use; D001853:Bone Marrow / Q000098:blood supply / Q000187:drug effects / Q000473:pathology*; D001943:Breast Neoplasms / Q000098:blood supply / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D060828:Induction Chemotherapy / Q000379:methods; D015470:Leukemia, Myeloid, Acute / Q000175:diagnosis* / Q000188:drug therapy / Q000401:mortality / Q000473:pathology; D008297:Male; D055806:Microvessels / Q000187:drug effects / Q000473:pathology*; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000175:diagnosis* / Q000188:drug therapy / Q000401:mortality / Q000473:pathology; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis* / Q000188:drug therapy / Q000401:mortality / Q000473:pathology; D011379:Prognosis; D016019:Survival Analysis; D016896:Treatment Outcome
chemical_list: D000970:Antineoplastic Agents
keywords: Acute leukemia; Angiogenesis; Clinical outcome; Microvessel density; Prognosis