title: Selecting DNA aptamers for endotoxin separation.
abstract: OBJECTIVE
  To select aptamers for endotoxin separation from a 75-nucleotide single-stranded DNA random library using systematic evolution of ligands by exponential enrichment.
  
  
  RESULTS
  After 15 rounds of selection, the final pool of aptamers was specific to endotoxin. Structural analysis of aptamers that appeared more than once suggested that one aptamer can form a G-quartet structure. Tests for binding affinity and specificity showed that this aptamer exhibited a high affinity for endotoxin. Using this aptamer, aptamer-magnetic beads were designed to separate endotoxin.
  
  
  CONCLUSIONS
  Using these aptamer-magnetic beads, a new method to separate endotoxin was developed to enable specific separation of endotoxin that can be applied to drug and food products.
authors.affiliation: College of Pharmaceutical Science, ZheJiang University, Hangzhou, 310013, China, bioph@zjut.edu.cn.
authors.forename: GuoQing
authors.initials: G
authors.lastname: Ying
authors.forename: FangFang
authors.initials: F
authors.lastname: Zhu
authors.forename: Yu
authors.initials: Y
authors.lastname: Yi
authors.forename: JianShu
authors.initials: J
authors.lastname: Chen
authors.forename: JianFeng
authors.initials: J
authors.lastname: Mei
authors.forename: YanLu
authors.initials: Y
authors.lastname: Zhang
authors.forename: ShuQing
authors.initials: S
authors.lastname: Chen
mesh_terms: D052157:Aptamers, Nucleotide / Q000302:isolation & purification* / Q000378:metabolism*; D019020:Directed Molecular Evolution; D004731:Endotoxins / Q000302:isolation & purification* / Q000378:metabolism*; D052156:SELEX Aptamer Technique
chemical_list: D052157:Aptamers, Nucleotide; D004731:Endotoxins