title: Genomic characterization of G3P[6], G4P[6] and G4P[8] human rotaviruses from Wuhan, China: Evidence for interspecies transmission and reassortment events.
abstract: We report here the whole genomic analyses of two G4P[6] (RVA/Human-wt/CHN/E931/2008/G4P[6], RVA/Human-wt/CHN/R1954/2013/G4P[6]), one G3P[6] (RVA/Human-wt/CHN/R946/2006/G3P[6]) and one G4P[8] (RVA/Human-wt/CHN/E2484/2011/G4P[8]) group A rotavirus (RVA) strains detected in sporadic cases of diarrhea in humans in the city of Wuhan, China. All the four strains displayed a Wa-like genotype constellation. Strains E931 and R1954 shared a G4-P[6]-I1-R1-C1-M1-A8-N1-T1-E1-H1 constellation, whilst the 11 gene segments of strains R946 and E2484 were assigned to G3-P[6]-I1-R1-C1-M1-A1-N1-T1-E1-H1 and G4-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1 genotypes, respectively. Phylogenetically, the VP7 gene of R946, NSP3 gene of E931, and 10 of 11 gene segments of E2484 (except for VP7 gene) belonged to lineages of human RVAs. On the other hand, based on available data, it was difficult to ascertain porcine or human origin of VP3 genes of strains E931 and R946, and NSP2 genes of strains R946 and R1954. The remaining genes of E2484, E931, R946 and R1954 were close to those of porcine RVAs from China, and/or porcine-like human RVAs. Taken together, our observations suggested that strain R1954 might have been derived from porcine RVAs, whilst strains R946 and E931 might be reassortants possessing human RVA-like gene segments on a porcine RVA genetic backbone. Strain E2484 might be derived from reassortment events involving acquisition of a porcine-like VP7 gene by a Wa-like human RVA strain. The present study provided important insights into zoonotic transmission and complex reassortment events involving human and porcine RVAs, reiterating the significance of whole-genomic analysis of RVA strains.
authors.affiliation: Virology Section, Wuhan Centers for Disease Prevention and Control, Wuhan, Hubei Province, PR China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Virology Section, Wuhan Centers for Disease Prevention and Control, Wuhan, Hubei Province, PR China. Electronic address: wyuanh1@hotmail.com.
authors.forename: Yuan-Hong
authors.initials: YH
authors.lastname: Wang
authors.affiliation: Department of Biomedical Sciences, Ross University School of Veterinary Medicine, Saint Kitts and Nevis; Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo, Japan.
authors.forename: Souvik
authors.initials: S
authors.lastname: Ghosh
authors.affiliation: Virology Section, Wuhan Centers for Disease Prevention and Control, Wuhan, Hubei Province, PR China.
authors.forename: Wei-Feng
authors.initials: WF
authors.lastname: Tang
authors.affiliation: Virology Section, Wuhan Centers for Disease Prevention and Control, Wuhan, Hubei Province, PR China.
authors.forename: Bei-Bei
authors.initials: BB
authors.lastname: Pang
authors.affiliation: Virology Section, Wuhan Centers for Disease Prevention and Control, Wuhan, Hubei Province, PR China.
authors.forename: Man-Qing
authors.initials: MQ
authors.lastname: Liu
authors.affiliation: Virology Section, Wuhan Centers for Disease Prevention and Control, Wuhan, Hubei Province, PR China.
authors.forename: Jin-Song
authors.initials: JS
authors.lastname: Peng
authors.affiliation: Virology Section, Wuhan Centers for Disease Prevention and Control, Wuhan, Hubei Province, PR China.
authors.forename: Dun-Jin
authors.initials: DJ
authors.lastname: Zhou
authors.affiliation: Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo, Japan. Electronic address: nkobayas@sapmed.ac.jp.
authors.forename: Nobumichi
authors.initials: N
authors.lastname: Kobayashi
mesh_terms: D000483:Alleles; D019943:Amino Acid Substitution; D000818:Animals; D016679:Genome, Viral; D023281:Genomics; D005838:Genotype; D006801:Humans; D009154:Mutation; D010802:Phylogeny; D016865:Reassortant Viruses; D012401:Rotavirus / Q000145:classification / Q000235:genetics*; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000382:microbiology* / Q000635:transmission; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D014764:Viral Proteins
keywords: Human rotavirus; Interspecies transmission; Porcine-like rotavirus; Reassortment; Whole genome analysis