title: A cyanine-modified upconversion nanoprobe for NIR-excited imaging of endogenous hydrogen peroxide signaling in vivo.
abstract: Endogenous hydrogen peroxide is an important parameter associated with cellular signal transduction and homeostasis. However, abnormal H2O2 regulation in live systems has been implicated in many pathological conditions. Monitoring this signal in live systems is essential but challenging because current H2O2 probes are impractical for efficient bio-imaging due to UV/visible light as the excitation source. We herein present a novel design based on an organic fluorophore-attached lanthanide-doped upconversion nanoprobe (CYD1-UCNPs) for selective UCL detection of H2O2. This nanoprobe represents the next-generation imaging tool that features a robust UCL "turn-on" response to H2O2 with NIR-excited ratiometric signals and has potential applications in ratiometric UCL imaging of endogenous H2O2 generating in living cells and whole-body animals.
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798, Singapore.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Institute of Advanced Materials (IAM) and Jiangsu-Singapore Joint Research Center for Organic/Bio-Electronics & Information Displays, Nanjing Tech University (NanjingTech), Nanjing 211816, China.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Pei
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798, Singapore.
authors.forename: Xiao
authors.initials: X
authors.lastname: Zhang
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798, Singapore.
authors.forename: Wangqiao
authors.initials: W
authors.lastname: Chen
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798, Singapore.
authors.forename: Jiansheng
authors.initials: J
authors.lastname: Wu
authors.affiliation: College of Science, Nanjing Tech University (NanjingTech), Nanjing 211816, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Yao
authors.affiliation: Institute of Advanced Materials (IAM) and Jiangsu-Singapore Joint Research Center for Organic/Bio-Electronics & Information Displays, Nanjing Tech University (NanjingTech), Nanjing 211816, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Huang
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798, Singapore.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Institute of Advanced Materials (IAM) and Jiangsu-Singapore Joint Research Center for Organic/Bio-Electronics & Information Displays, Nanjing Tech University (NanjingTech), Nanjing 211816, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798, Singapore; Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, 637551, Singapore. Electronic address: JoachimLoo@ntu.edu.sg.
authors.forename: Joachim Say
authors.initials: JS
authors.lastname: Chye Loo
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798, Singapore; Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore 637371, Singapore. Electronic address: qczhang@ntu.edu.sg.
authors.forename: Qichun
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002232:Carbocyanines / Q000737:chemistry*; D004396:Coloring Agents / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000378:metabolism*; D051379:Mice; D057054:Molecular Imaging / Q000379:methods*; D015336:Molecular Probe Techniques; D015335:Molecular Probes / Q000032:analysis / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D000067996:RAW 264.7 Cells; D019265:Spectroscopy, Near-Infrared / Q000379:methods*
chemical_list: D002232:Carbocyanines; D004396:Coloring Agents; D015335:Molecular Probes; C000657:cyanine dye 1; D006861:Hydrogen Peroxide
keywords: Hydrogen peroxide; Immune response; NIR-excited imaging; Ratiometric; Upconversion luminescence