title: [Biocompatibility of poly-L-lactic acid/Bioglass-guided bone regeneration membranes processed with oxygen plasma].
abstract: OBJECTIVE
  To prepare and characterize a nano-scale fibrous hydrophilic poly-L-lactic acid/ Bioglass (PLLA/BG) composite membrane and evaluate its biocompatibility as a composite membrane for guiding bone regeneration (GBR).
  
  
  METHODS
  PLLA/BG-guided bone regeneration membrane was treated by oxygen plasma to improved its hydrophilicity. The growth of MG-63 osteoblasts on the membrane was observed using Hoechst fluorescence staining, and the biocompatibility of the membrane was evaluated by calculating the cells adhesion rate and proliferation rate. Osteogenesis of MG-63 cells was assessed by detecting alkaline phosphatase (ALP), and the formation of calcified nodules and cell morphology changes were observed using scanning electron microscope (SEM).
  
  
  RESULTS
  The cell adhesion rates of PLLA/BG-guided bone regeneration membrane treated with oxygen plasma were (30.570±0.96)%, (47.27±0.78)%, and (66.78±0.69)% at 1, 3, and 6 h, respectively, significantly higher than those on PLLA membrane and untreated PLLA/BG membrane (P<0.01). The cell proliferation rates on the 3 membranes increased with time, but highest on oxygen plasma-treated PLLA/BG membrane (P<0.01). Hoechst fluorescence staining revealed that oxygen plasma treatment of the PLLA/BG membrane promoted cell adhesion. The membranes with Bioglass promoted the matrix secretion of the osteoblasts. Under SEM, the formation of calcified nodules and spindle-shaped cell morphology were observed on oxygen plasma-treated PLLA/BG membrane.
  
  
  CONCLUSIONS
  Oxygen plasma-treated PLLA/BG composite membrane has good biocompatibility and can promote adhesion, proliferation and osteogenesis of the osteoblasts.
authors.affiliation: Guangdong Provincial Stomatological Hospital Affiliated to Southern Medical University, Guangzhou 510280, China. E-mail: xiaoxin-909@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Fang
authors.forename: Shu-Guang
authors.initials: SG
authors.lastname: Zeng
authors.forename: Wen-Feng
authors.initials: WF
authors.lastname: Gao
mesh_terms: D000469:Alkaline Phosphatase; D001672:Biocompatible Materials / Q000737:chemistry*; D001861:Bone Regeneration; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D002516:Ceramics; D048091:Guided Tissue Regeneration; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology; D010012:Osteogenesis; D010100:Oxygen; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; C022107:Bioglass; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D000469:Alkaline Phosphatase; D010100:Oxygen