title: Induction of c-Jun by air particulate matter (PM₁₀) of Mexico city: Participation of polycyclic aromatic hydrocarbons.
abstract: The carcinogenic potential of urban particulate matter (PM) has been partly attributed to polycyclic aromatic hydrocarbons (PAHs) content, which activates the aryl hydrocarbon receptor (AhR). Here we report the effect of PM with an aerodynamic size of 10 μm (PM10) on the induction of AhR pathway in A549 cells, evaluating its downstream targets CYP1B1, IL-6, IL-8 and c-Jun. Significant increases in CYP1B1 protein and enzyme activity; IL-6 and IL-8 secretion and c-Jun protein were found in response to PM10. The formation of PAH-DNA adducts was also detected. The involvement of AhR pathway was confirmed with Resveratrol as AhR antagonist, which reversed CYP1B1 and c-Jun induction. Nevertheless, in IL-6 and IL-8 secretion, the Resveratrol was ineffective, suggesting an effect independent of this pathway. Considering the role of c-Jun in oncogenesis, its induction by PM may be contributing to its carcinogenic potential through induction of AhR pathway by PAHs present in PM10.
authors.affiliation: Subdirección de Investigación Básica, Instituto Nacional de Cancerología, Av. San Fernando No. 22, Col Sección XVI. C.P. 14080. Delegación Tlalpan, México, D.F., Mexico.
authors.forename: Martha Estela
authors.initials: ME
authors.lastname: Salcido-Neyoy
authors.affiliation: Subdirección de Investigación Básica, Instituto Nacional de Cancerología, Av. San Fernando No. 22, Col Sección XVI. C.P. 14080. Delegación Tlalpan, México, D.F., Mexico.
authors.forename: Yesennia
authors.initials: Y
authors.lastname: Sánchez-Pérez
authors.affiliation: Department of Paediatrics, University of Alberta, 3-591 ECHA, 11405 87th Ave, NW, Edmonton T6G 1C9, Canada.
authors.forename: Alvaro Román
authors.initials: AR
authors.lastname: Osornio-Vargas
authors.affiliation: Departamento de Medicina Genómica y Toxicología Ambiental, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, A.P. 70228. Ciudad Universitaria. 04510, México, D.F., Mexico.
authors.forename: María Eugenia
authors.initials: ME
authors.lastname: Gonsebatt
authors.affiliation: Laboratorio de Genómica Funcional del Cáncer, Instituto Nacional de Medicina Genómica, Periférico Sur, No.4809. Col. Arenal Tepepan, C.P. 14610. Delegación Tlalpan, México, D.F., Mexico.
authors.forename: Jorge
authors.initials: J
authors.lastname: Meléndez-Zajgla
authors.affiliation: Subdirección de Investigación Básica, Instituto Nacional de Cancerología, Av. San Fernando No. 22, Col Sección XVI. C.P. 14080. Delegación Tlalpan, México, D.F., Mexico.
authors.forename: Rocío
authors.initials: R
authors.lastname: Morales-Bárcenas
authors.affiliation: Departamento de Medicina Genómica y Toxicología Ambiental, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, A.P. 70228. Ciudad Universitaria. 04510, México, D.F., Mexico.
authors.forename: Pavel
authors.initials: P
authors.lastname: Petrosyan
authors.affiliation: Departamento de Medicina Genómica y Toxicología Ambiental, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, A.P. 70228. Ciudad Universitaria. 04510, México, D.F., Mexico.
authors.forename: Edith Danny
authors.initials: ED
authors.lastname: Molina-Servin
authors.affiliation: Instituto Mexicano del Petróleo, Eje Central Lázaro Cárdenas 152. San Bartolo Atepehuacan, C.P. 07730, México, D.F., Mexico.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Vega
authors.affiliation: Subdirección de Investigación Básica, Instituto Nacional de Cancerología, Av. San Fernando No. 22, Col Sección XVI. C.P. 14080. Delegación Tlalpan, México, D.F., Mexico.
authors.forename: Natalia
authors.initials: N
authors.lastname: Manzano-León
authors.affiliation: Subdirección de Investigación Básica, Instituto Nacional de Cancerología, Av. San Fernando No. 22, Col Sección XVI. C.P. 14080. Delegación Tlalpan, México, D.F., Mexico. Electronic address: claudia.garciac@salud.gob.mx.
authors.forename: Claudia M
authors.initials: CM
authors.lastname: García-Cuellar
mesh_terms: D000393:Air Pollutants / Q000633:toxicity*; D045744:Cell Line, Tumor; D002947:Cities; D065633:Cytochrome P-450 CYP1B1 / Q000378:metabolism; D018736:DNA Adducts / Q000378:metabolism; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D008800:Mexico; D052638:Particulate Matter / Q000633:toxicity*; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism / Q000633:toxicity*; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism*; D018336:Receptors, Aryl Hydrocarbon / Q000037:antagonists & inhibitors / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology
chemical_list: D000393:Air Pollutants; D018736:DNA Adducts; C508600:IL6 protein, human; D015850:Interleukin-6; D016209:Interleukin-8; D052638:Particulate Matter; D011084:Polycyclic Aromatic Hydrocarbons; D016755:Proto-Oncogene Proteins c-jun; D018336:Receptors, Aryl Hydrocarbon; D013267:Stilbenes; C095911:polycyclic aromatic hydrocarbons-DNA adduct; C585496:CYP1B1 protein, human; D065633:Cytochrome P-450 CYP1B1; D000077185:Resveratrol
keywords: Particulate matter; aryl hydrocarbon receptor; cJun; carcinogenic potential; polycyclic aromatic hydrocarbons