title: Mesophilic hydrogen production in acidogenic packed-bed reactors (APBR) using raw sugarcane vinasse as substrate: Influence of support materials.
abstract: Bio-hydrogen production from sugarcane vinasse in anaerobic up-flow packed-bed reactors (APBR) was evaluated. Four types of support materials, expanded clay (EC), charcoal (Ch), porous ceramic (PC), and low-density polyethylene (LDP) were tested as support for biomass attachment. APBR (working volume - 2.3 L) were operated in parallel at a hydraulic retention time of 24 h, an organic loading rate of 36.2 kg-COD m(-3) d(-1), at 25 °C. Maximum volumetric hydrogen production values of 509.5, 404, 81.4 and 10.3 mL-H2 d(-1) L(-1)reactor and maximum yields of 3.2, 2.6, 0.4 and 0.05 mol-H2 mol(-1) carbohydrates total, were observed during the monitoring of the reactors filled with LDP, EC, Ch and PC, respectively. Thus, indicating the strong influence of the support material on H2 production. LDP was the most appropriate material for hydrogen production among the materials evaluated. 16S rRNA gene by Terminal Restriction Fragment Length Polymorphism (T-RFLP) analysis and scanning electron microscopy confirmed the selection of different microbial populations. 454-pyrosequencing performed on samples from APBR filled with LDP revealed the presence of hydrogen-producing organisms (Clostridium and Pectinatus), lactic acid bacteria and non-fermentative organisms.
authors.affiliation: CTBE: Brazilian Bioethanol Science and Technology Laboratory - CNPEM, Rua Giuseppe Máximo Scolfaro, 10.000 Bairro Guará, Barão Geraldo, 13.083-970 Campinas, SP, Brazil; Biological Processes Laboratory, Center for Research, Development and Innovation in Environmental Engineering, São Carlos School of Engineering (EESC), University of São Paulo (USP), Engenharia Ambiental - Bloco 4-F, Av. João Dagnone, 1100 - Santa Angelina, 13.563-120 São Carlos, SP, Brazil. Electronic address: djalma.ferraz@bioetanol.org.br.
authors.forename: Antônio Djalma
authors.initials: AD
authors.lastname: Nunes Ferraz Júnior
authors.affiliation: Laboratorio de Ecología Microbiana, Instituto de Investigaciones Biológicas Clemente Estable, Av. Italia 3318, Montevideo, Uruguay. Electronic address: cetchebehere@iibce.edu.uy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Etchebehere
authors.affiliation: Biological Processes Laboratory, Center for Research, Development and Innovation in Environmental Engineering, São Carlos School of Engineering (EESC), University of São Paulo (USP), Engenharia Ambiental - Bloco 4-F, Av. João Dagnone, 1100 - Santa Angelina, 13.563-120 São Carlos, SP, Brazil. Electronic address: zaiat@sc.usp.br.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Zaiat
mesh_terms: D000693:Anaerobiosis; D019149:Bioreactors / Q000382:microbiology*; D058448:Biota; D002264:Carboxylic Acids / Q000378:metabolism*; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D006859:Hydrogen / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D031786:Saccharum / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D013696:Temperature
chemical_list: D002264:Carboxylic Acids; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D006859:Hydrogen
keywords: 454-Pyrosequencing; Bio-hydrogen; Raw sugarcane vinasse; Real time PCR; Support materials