title: Storage of washed or irradiated red cells in AS-7 improves their in vitro characteristics.
abstract: BACKGROUND
  AS-7 is a new alkaline hypotonic red cell additive solution (AS) shown to improve red cell quality during storage compared with AS-1. We sought to compare red cells stored in AS-7 with those stored in SAGM using RCC that were either untreated, or washed or irradiated on day 14 of storage.
  
  
  METHODS
  A pooled and split study design was used to produce seven identical RCC (four in SAGM and three in AS-7). At day 14 following donation, two RCC (one in SAGM and one in AS-7) were gamma irradiated and three RCC (two in SAGM and one in AS-7) were washed and resuspended in either SAGM or AS-7. RCC were sampled for analysis throughout storage and at end of shelf life: day 28 for washed or irradiated and day 35 for untreated RCC.
  
  
  RESULTS
  For untreated, washed or irradiated RCC, those stored in AS-7 had lower haemolysis, red cell microvesicles and supernatant potassium content than RCC in SAGM. In addition, ATP levels and pH were better maintained in AS-7 RCC than in SAGM RCC.
  
  
  CONCLUSIONS
  These data suggest that the quality of these components may be improved by storage in AS-7 compared with SAGM.
authors.affiliation: Component Development Laboratory, NHS Blood and Transplant, Cambridge, UK.
authors.forename: S
authors.initials: S
authors.lastname: Proffitt
authors.affiliation: Component Development Laboratory, NHS Blood and Transplant, Cambridge, UK.
authors.forename: S
authors.initials: S
authors.lastname: Thomas
authors.affiliation: Haemonetics Corporation, Braintree, MA, USA.
authors.forename: I
authors.initials: I
authors.lastname: Swann
authors.affiliation: Haemonetics Corporation, Braintree, MA, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Popovsky
authors.affiliation: NHS Blood and Transplant, Oxford Centre, John Radcliffe Hospital, Oxford, UK.
authors.forename: D J
authors.initials: DJ
authors.lastname: Smith
authors.affiliation: NHS Blood and Transplant, Oxford Centre, John Radcliffe Hospital, Oxford, UK.|Radcliffe Department of Medicine, John Radcliffe Hospital, University of Oxford, Oxford, UK.
authors.forename: D J
authors.initials: DJ
authors.lastname: Roberts
authors.affiliation: Component Development Laboratory, NHS Blood and Transplant, Cambridge, UK.
authors.forename: R
authors.initials: R
authors.lastname: Cardigan
mesh_terms: D000225:Adenine / Q000737:chemistry; D000255:Adenosine Triphosphate / Q000378:metabolism; D001793:Blood Preservation / Q000379:methods*; D004912:Erythrocytes / Q000166:cytology* / Q000378:metabolism / Q000528:radiation effects; D005720:Gamma Rays; D005947:Glucose / Q000737:chemistry; D006461:Hemolysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008353:Mannitol / Q000737:chemistry; D012965:Sodium Chloride / Q000737:chemistry; D013997:Time Factors
chemical_list: C037839:ADSOL; D008353:Mannitol; D012965:Sodium Chloride; D000255:Adenosine Triphosphate; D005947:Glucose; D000225:Adenine
keywords: additive solution; blood components; blood processing; red cell components