title: In planta analysis of leaf mitochondrial superoxide and nitric oxide.
abstract: Superoxide (O2(-)) and nitric oxide (NO) are produced within plant mitochondria and may have signaling functions within the cell. Here we describe semiquantitative fluorescence imaging-based approaches to estimate the mitochondrial amount of these reactive and short-lived species within intact leaf tissue. We also outline a biochemical method using oxyhemoglobin to measure NO within a whole leaf tissue extract. This quantitative method, while not specifically evaluating mitochondrial localized NO, does nonetheless provide another independent measure of NO that can be useful.
authors.affiliation: Department of Biological Sciences, University of Toronto Scarborough, 1265 Military Trail, Toronto, ON, Canada, M1C1A4.
authors.forename: Marina
authors.initials: M
authors.lastname: Cvetkovska
authors.forename: Greg C
authors.initials: GC
authors.lastname: Vanlerberghe
mesh_terms: D018613:Microscopy, Confocal / Q000379:methods; D008928:Mitochondria / Q000737:chemistry / Q000378:metabolism*; D009569:Nitric Oxide / Q000032:analysis / Q000378:metabolism*; D061848:Optical Imaging / Q000379:methods; D010108:Oxyhemoglobins / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism*; D013481:Superoxides / Q000032:analysis / Q000378:metabolism*; D014026:Nicotiana / Q000737:chemistry / Q000378:metabolism*
chemical_list: D010108:Oxyhemoglobins; D013481:Superoxides; D009569:Nitric Oxide