title: Relationship between biomarkers of inflammation, oxidative stress and endothelial/microcirculatory function in successful aging versus healthy youth: a transversal study.
abstract: BACKGROUND
  There is a functional decline of endothelial- dependent vasodilatation in the aging process. The aims of this study were to investigate if various microcirculatory parameters could correlate to anthropometrical variables, oxidative stress and inflammatory biomarkers in successful aging and compare the results to young healthy controls.
  
  
  METHODS
  Healthy elderly women (HE, 74.0 ± 8.7 years, n = 11) and young controls (YC, 23.1 ± 3.6 years, n = 24) were evaluated through nailfold videocapillaroscopy (NVC), venous occlusion plethysmography (VOP) and laboratorial analysis. Functional capillary density (FCD) and diameters, maximum red blood cell velocity (RBCVmax) during the reactive hyperemia response/RBCVbaseline after 1 min arterial occlusion at the finger base, time to reach RBCVmax were determined by NVC, peak increment of forearm blood flow (FBF) during the reactive hyperemia response (%Hyper) and after 0.4 mg sublingual nitroglycerin (%Nitro) by VOP and lipidogram, fibrinogen, fasting and postload glucose, oxidized LDL-cholesterol (oxLDL), sICAM, sVCAM, sE-Selectin, interleukines 1 and 6 and TNF-α by laboratorial analysis. Correlations and linear multiple regression (LMR) between %Hyper, %Nitro, microcirculatory parameters, oxidative stress and inflammatory biomarkers were investigated.
  
  
  RESULTS
  sVCAM, sE-Selectin and oxLDL were higher and RBCVmax/RBCVbaseline and %Hyper lower in HE, while %Nitro and FCD remained unchanged. Fibrinogen, LDL-cholesterol, oxLDL correlated negatively to %Hyper while sVCAM correlated negatively to %Hyper and RBCVmax/RBCVbaseline. Healthy aged women presented dilated capillaries with sustained perfusion and endothelial dysfunction with preserved vascular smooth muscle reactivity. Fibrinogen, LDL-cholesterol, oxidized-LDL and sVCAM correlated negatively to endothelial function but not to microcirculatory parameters. Oxidized-LDL and sVCAM could determine %Hyper through LMR.
  
  
  CONCLUSIONS
  Oxidized-LDL and sVCAM might be used as endothelial dysfunction biomarkers for elderly with normal cardiovascular risk factors.
authors.affiliation: Clinical and Experimental Research Laboratory on Vascular Biology (BioVasc), Biomedical Center, State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil. dbottino1@yahoo.com.br.
authors.forename: Daniel Alexandre
authors.initials: DA
authors.lastname: Bottino
authors.affiliation: UNATI, State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil. flaviaglopes@ibest.com.br.
authors.forename: Flávia Gomes
authors.initials: FG
authors.lastname: Lopes
authors.affiliation: UNATI, State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil. olivfj@gmail.com.
authors.forename: Francisco José
authors.initials: FJ
authors.lastname: de Oliveira
authors.affiliation: Clinical and Experimental Research Laboratory on Vascular Biology (BioVasc), Biomedical Center, State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil. anetemecenas@yahoo.com.br.
authors.forename: Anete de Souza
authors.initials: Ade S
authors.lastname: Mecenas
authors.affiliation: Clinical and Experimental Research Laboratory on Vascular Biology (BioVasc), Biomedical Center, State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil. rclapauch@uol.com.br.
authors.forename: Ruth
authors.initials: R
authors.lastname: Clapauch
authors.affiliation: Clinical and Experimental Research Laboratory on Vascular Biology (BioVasc), Biomedical Center, State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil. eliete_bouskela@yahoo.com.br.
authors.forename: Eliete
authors.initials: E
authors.lastname: Bouskela
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000502:physiology*; D015415:Biomarkers / Q000097:blood*; D002318:Cardiovascular Diseases / Q000378:metabolism* / Q000503:physiopathology; D004730:Endothelium, Vascular / Q000503:physiopathology*; D005260:Female; D064368:Healthy Volunteers; D006801:Humans; D007249:Inflammation / Q000097:blood*; D008297:Male; D008833:Microcirculation / Q000502:physiology*; D018384:Oxidative Stress; D012307:Risk Factors; D014664:Vasodilation / Q000502:physiology*; D055815:Young Adult
chemical_list: D015415:Biomarkers