title: UV-activated 7-dehydrocholesterol-coated titanium implants promote differentiation of human umbilical cord mesenchymal stem cells into osteoblasts.
abstract: Vitamin D metabolites are essential for bone regeneration and mineral homeostasis. The vitamin D precursor 7-dehydrocholesterol can be used after UV irradiation to locally produce active vitamin D by osteoblastic cells. Furthermore, UV-irradiated 7-dehydrocholesterol is a biocompatible coating for titanium implants with positive effects on osteoblast differentiation. In this study, we examined the impact of titanium implants surfaces coated with UV-irradiated 7-dehydrocholesterol on the osteogenic differentiation of human umbilical cord mesenchymal stem cells. First, the synthesis of cholecalciferol (D3) was achieved through the incubation of the UV-activated 7-dehydrocholesterol coating for 48 h at 23℃. Further, we investigated in vitro the biocompatibility of this coating in human umbilical cord mesenchymal stem cells and its potential to enhance their differentiation towards the osteogenic lineage. Human umbilical cord mesenchymal stem cells cultured onto UV-irradiated 7-dehydrocholesterol-coated titanium implants surfaces, combined with osteogenic supplements, upregulated the gene expression of several osteogenic markers and showed higher alkaline phosphatase activity and calcein blue staining, suggesting increased mineralization. Thus, our results show that the use of UV irradiation on 7-dehydrocholesterol -treated titanium implants surfaces generates a bioactive coating that promotes the osteogenic differentiation of human umbilical cord mesenchymal stem cells, with regenerative potential for improving osseointegration in titanium-based bone anchored implants.
authors.affiliation: Department of Fundamental Biology and Health Sciences, Research Institute on Health Sciences (IUNICS), University of Balearic Islands, Palma de Mallorca, Spain; Instituto de Investigación Sanitaria de Palma, Palma de Mallorca, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Satué
authors.affiliation: Department of Fundamental Biology and Health Sciences, Research Institute on Health Sciences (IUNICS), University of Balearic Islands, Palma de Mallorca, Spain; Instituto de Investigación Sanitaria de Palma, Palma de Mallorca, Spain.
authors.forename: Joana M
authors.initials: JM
authors.lastname: Ramis
authors.affiliation: Department of Fundamental Biology and Health Sciences, Research Institute on Health Sciences (IUNICS), University of Balearic Islands, Palma de Mallorca, Spain; Instituto de Investigación Sanitaria de Palma, Palma de Mallorca, Spain marta.monjo@uib.es.
authors.forename: Marta
authors.initials: M
authors.lastname: Monjo
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000528:radiation effects; D003684:Dehydrocholesterols / Q000737:chemistry* / Q000494:pharmacology* / Q000528:radiation effects; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology*; D019736:Prostheses and Implants; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*; D014466:Ultraviolet Rays
chemical_list: D020099:Coated Materials, Biocompatible; D003684:Dehydrocholesterols; C016705:7-dehydrocholesterol; D014025:Titanium
keywords: 7-Dehydrocholesterol; UV irradiation; mesenchymal stem cells; osteogenic differentiation; titanium implant; vitamin D