title: Sperm abnormalities induced by pre-pubertal exposure to cyclophosphamide are effectively mitigated by Moringa oleifera leaf extract.
abstract: Moringa oleifera L. is a medicinal plant with potential antioxidant property. This study was aimed at investigating the chemoprotective effect of Moringa oleifera leaf extract (MOE) on cyclophosphamide (CP)-induced testicular toxicity. Two-week-old male Swiss albino mice were intraperitoneally injected with phosphate-buffered saline, 50 mg kg(-1) of CP and 25 mg kg(-1) of MOE. In combination treatment, mice were injected with 25 mg kg(-1) of MOE 24 h prior to CP injection, 24 h prior and post-CP injection and 24 h post-CP injection for 5 consecutive days (10 mg kg(-1) ). Six weeks later, mice were sacrificed to assess epididymal sperm parameters. MOE alone did not have any significant effect on sperm parameters. However, acute injection of CP resulted in significant decline in motility (P < 0.001), increase in head abnormality (P < 0.01) and DNA damage (P < 0.05). Combining MOE with CP increased the sperm density, motility and reduced head defect and DNA damage, irrespective of the schedule and dosage of MOE. Administration of MOE prior to CP significantly elevated the level of superoxide dismutase and catalase with concomitant decrease in lipid peroxidation in the testicular tissue. In conclusion, MOE may have potential benefit in reducing the loss of male gonadal function following chemotherapy.
authors.affiliation: Division of Clinical Embryology, Department of Obstetrics and Gynecology, Kasturba Medical College, Manipal University, Manipal, Karnataka, India.
authors.forename: G
authors.initials: G
authors.lastname: Nayak
authors.affiliation: Division of Clinical Embryology, Department of Obstetrics and Gynecology, Kasturba Medical College, Manipal University, Manipal, Karnataka, India.
authors.forename: A
authors.initials: A
authors.lastname: Vadinkar
authors.affiliation: Division of Clinical Embryology, Department of Obstetrics and Gynecology, Kasturba Medical College, Manipal University, Manipal, Karnataka, India.
authors.forename: S
authors.initials: S
authors.lastname: Nair
authors.affiliation: Department of Anatomy, Kasturba Medical College, Manipal University, Manipal, Karnataka, India.
authors.forename: S G
authors.initials: SG
authors.lastname: Kalthur
authors.affiliation: Department of Anatomy, Kasturba Medical College, Manipal University, Manipal, Karnataka, India.
authors.forename: A S
authors.initials: AS
authors.lastname: D'Souza
authors.affiliation: Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka, India.
authors.forename: P K
authors.initials: PK
authors.lastname: Shetty
authors.affiliation: Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka, India.
authors.forename: S
authors.initials: S
authors.lastname: Mutalik
authors.affiliation: Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka, India.
authors.forename: M M
authors.initials: MM
authors.lastname: Shetty
authors.affiliation: Division of Clinical Embryology, Department of Obstetrics and Gynecology, Kasturba Medical College, Manipal University, Manipal, Karnataka, India.
authors.forename: G
authors.initials: G
authors.lastname: Kalthur
authors.affiliation: Division of Clinical Embryology, Department of Obstetrics and Gynecology, Kasturba Medical College, Manipal University, Manipal, Karnataka, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Adiga
mesh_terms: D000818:Animals; D018906:Antineoplastic Agents, Alkylating / Q000633:toxicity*; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000187:drug effects / Q000378:metabolism; D003520:Cyclophosphamide / Q000633:toxicity*; D004249:DNA Damage / Q000187:drug effects*; D004822:Epididymis; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051379:Mice; D029588:Moringa oleifera; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves; D011627:Puberty; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000187:drug effects / Q000378:metabolism
chemical_list: D018906:Antineoplastic Agents, Alkylating; D000975:Antioxidants; D010936:Plant Extracts; D003520:Cyclophosphamide; D002374:Catalase; D013482:Superoxide Dismutase
keywords: Chemoprotection; Moringa oleifera; cyclophosphamide; sperm chromatin; testicular toxicity