title: A mitochondrial targeted two-photon iridium(III) phosphorescent probe for selective detection of hypochlorite in live cells and in vivo.
abstract: Endogenous hypochlorite ion (ClO(-)) is a highly reactive oxygen species (ROS) that is produced from hydrogen peroxide and chloride ions catalyzed by myeloperoxidase (MPO). And mitochondrion is one of the major sources of ROS including ClO(-). In the present work, a two-photon phosphorescent probe for ClO(-) in mitochondria was developed. An iridium(III) complex bearing a diaminomaleonitrile group as ClO(-) reactive moiety specifically responded to ClO(-) over other ions and ROSs. When the probe was reacted with ClO(-) to form an oxidized carboxylate product, a significant enhancement in phosphorescence intensity was observed under one-photon (402 nm) and two-photon (750 nm) excitation, with a two-photon absorption cross-section of 78.1 GM at 750 nm. More importantly, ICP-MS results and cellular images co-stained with Mito-tracker Green demonstrated that this probe possessed high specificity for mitochondria. This probe was applied in the one- and two-photon imaging of ClO(-) in vitro and in vivo. The results suggested endotoxin lipopolysaccharide (LPS) induced ClO(-) mostly generated in the liver of zebrafish.
authors.affiliation: MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, PR China.
authors.forename: Guanying
authors.initials: G
authors.lastname: Li
authors.affiliation: MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Lin
authors.affiliation: MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, PR China.
authors.forename: Lingli
authors.initials: L
authors.lastname: Sun
authors.affiliation: School of Physics and Engineering, Sun Yat-Sen University, Guangzhou 510275, PR China.
authors.forename: Changsheng
authors.initials: C
authors.lastname: Feng
authors.affiliation: MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, PR China.
authors.forename: Pingyu
authors.initials: P
authors.lastname: Zhang
authors.affiliation: MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, PR China.
authors.forename: Bole
authors.initials: B
authors.lastname: Yu
authors.affiliation: MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, PR China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Wen
authors.affiliation: School of Physics and Engineering, Sun Yat-Sen University, Guangzhou 510275, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, PR China.
authors.forename: Liangnian
authors.initials: L
authors.lastname: Ji
authors.affiliation: MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, PR China. Electronic address: ceschh@mail.sysu.edu.cn.
authors.forename: Hui
authors.initials: H
authors.lastname: Chao
mesh_terms: D000818:Animals; D002460:Cell Line; D006801:Humans; D006997:Hypochlorous Acid / Q000032:analysis*; D007495:Iridium / Q000494:pharmacology*; D057230:Limit of Detection; D049449:Luminescence; D051379:Mice; D008928:Mitochondria / Q000187:drug effects*; D015335:Molecular Probes; D017785:Photons; D015027:Zebrafish
chemical_list: D015335:Molecular Probes; D007495:Iridium; D006997:Hypochlorous Acid
keywords: Hypochlorite; Iridium(III) complex; Mitochondria; Two-photon probe; Zebrafish