title: Genetic diversity of Plasmodium falciparum isolates based on MSP-1 and MSP-2 genes from Kolla-Shele area, Arbaminch Zuria District, southwest Ethiopia.
abstract: BACKGROUND
  The genetic diversity of Plasmodium falciparum has been extensively studied in various countries. However, limited data are available from Ethiopia. This study was conducted to evaluate the extent of genetic diversity of P. falciparum in Kolla-Shele, in the southwest of Ethiopia.
  
  
  METHODS
  A total of 88 isolates from patients with uncomplicated P. falciparum attending Kolla-Shele Health Centre was collected from September to December, 2008. After extraction of DNA by Chelex method, the samples were genotyped by using nested-PCR of msp1 (block 2) and msp2 (block 3) including their allelic families: K1, MAD20, RO33 and FC27, 3D7/IC1, respectively.
  
  
  RESULTS
  Allelic variation in both msp1 and msp2 were identified in the 88 blood samples. For msp1 67% (59/88) and msp2 44% (39/88) were observed. K1 was the predominant msp1 allelic family observed in 33.9% (20/59) of the samples followed by RO33 and MAD20. Of the msp2 allelic family 3D7/IC1 showed higher frequency (21.5%) compared to FC27 (10.3%). A total of twenty-three alleles were detected; of which, eleven were from msp2 and twelve from msp2 genes. Fifty-nine percent of isolates had multiple genotypes and the overall mean multiplicity of infection was 1.8 (95% CI: 1.48-2.04). The heterozygosity index was 0.79 and 0.54for msp1 and msp2, respectively. There was no statically significant difference in the multiplicity of infection by either age or parasite density (P > 0.05).
  
  
  CONCLUSIONS
  This genetic diversity study showed the presence of five allelic types in the study area, with dominance K1 in the msp1 family and 3D7/IC1 in the msp2 family. Multiple infections were observed in nearly 60% of the samples.
authors.affiliation: Ethiopian Public Health Institute, Addis Ababa, Ethiopia. hussein_ehnri@yahoo.com.
authors.forename: Hussein
authors.initials: H
authors.lastname: Mohammed
authors.affiliation: Department of Laboratory Sciences, Addis Ababa University, Addis Ababa, Ethiopia. mindaye.tedla@gmail.com.
authors.forename: Tedla
authors.initials: T
authors.lastname: Mindaye
authors.affiliation: Department of Laboratory Sciences, Addis Ababa University, Addis Ababa, Ethiopia. meseretbt@gmail.com.
authors.forename: Meseret
authors.initials: M
authors.lastname: Belayneh
authors.affiliation: Ethiopian Public Health Institute, Addis Ababa, Ethiopia. eyobmk@yahoo.com.
authors.forename: Moges
authors.initials: M
authors.lastname: Kassa
authors.affiliation: Ethiopian Public Health Institute, Addis Ababa, Ethiopia. ashyaega@yahoo.com.
authors.forename: Ashenafi
authors.initials: A
authors.lastname: Assefa
authors.affiliation: Ethiopian Public Health Institute, Addis Ababa, Ethiopia. meknnta@gmail.com.
authors.forename: Mekonnen
authors.initials: M
authors.lastname: Tadesse
authors.affiliation: Ethiopian Public Health Institute, Addis Ababa, Ethiopia. adugnawayessa@gmail.com.
authors.forename: Adugna
authors.initials: A
authors.lastname: Woyessa
authors.affiliation: Ethiopian Public Health Institute, Addis Ababa, Ethiopia. mtes2003@gmail.com.
authors.forename: Tesfaye
authors.initials: T
authors.lastname: Mengesha
authors.affiliation: Ethiopian Public Health Institute, Addis Ababa, Ethiopia. amhak@gmail.com.
authors.forename: Amha
authors.initials: A
authors.lastname: Kebede
mesh_terms: D000293:Adolescent; D000483:Alleles; D000953:Antigens, Protozoan / Q000235:genetics*; D002675:Child, Preschool; D005002:Ethiopia; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007223:Infant; D016778:Malaria, Falciparum / Q000469:parasitology*; D008297:Male; D020066:Merozoite Surface Protein 1 / Q000235:genetics*; D010963:Plasmodium falciparum / Q000235:genetics*; D016133:Polymerase Chain Reaction; D015800:Protozoan Proteins / Q000235:genetics*; D055815:Young Adult
chemical_list: D000953:Antigens, Protozoan; D020066:Merozoite Surface Protein 1; D015800:Protozoan Proteins; C065974:merozoite surface protein 2, Plasmodium