title: Characterization of 19 Genes Encoding Membrane-Bound Fatty Acid Desaturases and their Expression Profiles in Gossypium raimondii Under Low Temperature.
abstract: To produce unsaturated fatty acids, membrane-bound fatty acid desaturases (FADs) can be exploited to introduce double bonds into the acyl chains of fatty acids. In this study, 19 membrane-bound FAD genes were identified in Gossypium raimondii through database searches and were classified into four different subfamilies based on phylogenetic analysis. All 19 membrane-bound FAD proteins shared three highly conserved histidine boxes, except for GrFAD2.1, which lost the third histidine box in the C-terminal region. In the G. raimondii genome, tandem duplication might have led to the increasing size of the FAD2 cluster in the Omega Desaturase subfamily, whereas segmental duplication appeared to be the dominant mechanism for the expansion of the Sphingolipid and Front-end Desaturase subfamilies. Gene expression analysis showed that seven membrane-bound FAD genes were significantly up-regulated and that five genes were greatly suppressed in G. raimondii leaves exposed to low temperature conditions.
authors.affiliation: Department of Agronomy, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Agronomy, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Agronomy, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Qiuling
authors.initials: Q
authors.lastname: He
authors.affiliation: Department of Agronomy, Zhejiang University, Hangzhou, 310058, China; Department of Biotechnology and Genetic Engineering, Kohat University of Science and Technology, Kohat, 26000, Pakistan.
authors.forename: Muhammad Khan
authors.initials: MK
authors.lastname: Daud
authors.affiliation: Department of Agronomy, Zhejiang University, Hangzhou, 310058, China; Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing, 210095, China.
authors.forename: Jinhong
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Agronomy, Zhejiang University, Hangzhou, 310058, China; Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing, 210095, China.
authors.forename: Shuijin
authors.initials: S
authors.lastname: Zhu
mesh_terms: D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics; D002462:Cell Membrane / Q000201:enzymology*; D032461:Chromosomes, Plant / Q000235:genetics; D003080:Cold Temperature; D017124:Conserved Sequence / Q000235:genetics; D044943:Fatty Acid Desaturases / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D003368:Gossypium / Q000201:enzymology* / Q000235:genetics*; D059372:Nucleotide Motifs / Q000235:genetics; D012275:Oryza / Q000201:enzymology / Q000235:genetics; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics; D036226:Seedlings / Q000235:genetics
chemical_list: D044943:Fatty Acid Desaturases