title: Effect of activated autologous platelet-rich plasma on proliferation and osteogenic differentiation of human adipose-derived stem cells in vitro.
abstract: To investigate whether activated autologous platelet-rich plasma (PRP) can promote proliferation and osteogenic differentiation of human adipose-derived stem cells (hASCs) in vitro. hASCs were isolated from lipo-aspirates, and characterized by specific cell markers and multilineage differentiation capacity after culturing to the 3(rd) passage. PRP was collected and activated from human peripheral blood of the same patient. Cultured hASCs were treated with normal osteogenic inductive media alone (group A, control) or osteogenic inductive media plus 5%, 10%, 20%, 40%PRP (group B, C, D, E, respectively). Cell proliferation was assessed by CCK-8 assay. mRNA expression of osteogenic marker genes including alkaline phosphatase (ALP), osteopontin (OPN), osteocalcin (OCN) and core binding factor alpha 1 (Cbfa1) were determined by Real-Time Quantitative PCR Analysis (qPCR). Data revealed that different concentrations of activated autologous PRP significantly promoted hASCs growth in the proliferation phase compared to the without PRP group and resulted in a dose-response relationship. At 7-d and 14-d time point of the osteogenic induced stage, ALP activity in PRP groups gradually increased with the increasing of concentrations of PRP and showed that dose-response relationship. At 21-d time point of the osteogenic induced stage, PRP groups make much more mineralization and mRNA relative expression of ALP, OPN, OCN and Cbfa1 than that without PRP groups and show that dose-response relationship. This study indicated that different concentrations of activated autologous PRP can promote cell proliferation at earlier stage and promote osteogenic differentiation at later stage of hASCs in vitro. Moreover, it displayed a dose-dependent effect of activated autologous PRP on cell proliferation and osteogenic differentiation of hASCs in vitro.
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Gannan Medical University Ganzhou 341000, China.
authors.forename: Fang-Tian
authors.initials: FT
authors.lastname: Xu
authors.affiliation: Department of Plastic and Aesthetic Surgery, The Affiliated Nanning First People's Hospital of Guangxi Medical University Nanning 530022, China.
authors.forename: Hong-Mian
authors.initials: HM
authors.lastname: Li
authors.affiliation: Department of Orthopedic Surgery, Guangzhou General Hospital of PLA Guangzhou 510180, China.
authors.forename: Qing-Shui
authors.initials: QS
authors.lastname: Yin
authors.affiliation: Department of Hepatobiliary and Gland Surgery, The Affiliated Nanning First People's Hospital of Guangxi Medical University Nanning 530022, China.
authors.forename: Zhi-Jie
authors.initials: ZJ
authors.lastname: Liang
authors.affiliation: Department of Hepatobiliary and Gland Surgery, The Affiliated Nanning First People's Hospital of Guangxi Medical University Nanning 530022, China.
authors.forename: Min-Hong
authors.initials: MH
authors.lastname: Huang
authors.affiliation: Department of Plastic and Aesthetic Surgery, The Affiliated Nanning First People's Hospital of Guangxi Medical University Nanning 530022, China.
authors.forename: Guang-Yi
authors.initials: GY
authors.lastname: Chi
authors.affiliation: Department of Plastic and Aesthetic Surgery, The Affiliated Nanning First People's Hospital of Guangxi Medical University Nanning 530022, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Plastic and Reconstructive Surgery, Zhujiang Hospital of Southern Medical University Guangzhou 510282, China.
authors.forename: Da-Lie
authors.initials: DL
authors.lastname: Liu
authors.affiliation: Department of Plastic and Reconstructive Surgery, Zhujiang Hospital of Southern Medical University Guangzhou 510282, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Nan
keywords: Adipose-derived stem cells; cell proliferation; cell therapy; induced differentiation; osteogenic differentiation; platelet-rich plasma