title: [The capacity and biological characteristic of the p75 neurotrophin receptor-positive ectomesenchymal stem cell in vitro].
abstract: OBJECTIVE
  To investigate the biological characteristic of ectomesenchymal stem cells (EMSC) and the differentiation of p75(+)EMSC and influencing factors.
  
  
  METHODS
  The immunohistochemical staining method was used to observe the migration of EMSC from 12.5 d SD rat embryonic facial process. Then EMSC was labeled by p75 neurotrophin receptor, and the cell cycle and stem cell surface antigens of the p75(+) EMSC was examined.
  
  
  RESULTS
  Immunohistochemical staining showed that stem cells from the cranial neural crest migrated to embryonic rat facial process at 12.5 days. The sorting rate of the p75(+)EMSC was 6.1%. The proportion of the p75(+)EMSCs' S/G2/M phase was stable during subculture. The special substances CD29, CD146 and Stro-1 were marked for p75(+)EMSC, and the expressions of the markers were all higher (> 90%) in p75(+) EMSC.
  
  
  CONCLUSIONS
  The embryonic tooth did not start to grow on the conception 12.5 days of SD rats. The p75(+) EMSC after sorting had stable proliferation ability and had stem cell characteristics during subculture and didn't start differentiation.
authors.affiliation: Department of Stomatology, The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Manzhu
authors.initials: M
authors.lastname: Zhao
authors.forename: Xiujie
authors.initials: X
authors.lastname: Wen
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.forename: Kun
authors.initials: K
authors.lastname: Yang
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.affiliation: Email: 1468492690@qq.com.
authors.forename: Ming
authors.initials: M
authors.lastname: Tang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002453:Cell Cycle; D002454:Cell Differentiation; D002455:Cell Division; D002465:Cell Movement; D066298:In Vitro Techniques; D059630:Mesenchymal Stem Cells / Q000737:chemistry / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020800:Receptor, Nerve Growth Factor
chemical_list: D015415:Biomarkers; D020800:Receptor, Nerve Growth Factor