title: Dendrimeric siRNA for Efficient Gene Silencing.
abstract: Programmable molecular self-assembly of siRNA molecules provides precisely controlled generation of dendrimeric siRNA nanostructures. The second-generation dendrimers of siRNA can be effectively complexed with a low-molecular-weight, cationic polymer (poly(β-amino ester), PBAE) to generate stable nanostructures about 160 nm in diameter via strong electrostatic interactions. Condensation and gene silencing efficiencies increase with the increased generation of siRNA dendrimers due to a high charge density and structural flexibility.
authors.affiliation: Department of Biological Sciences, Department of Materials Science and Engineering, KI for NanoCentury (KINC CNiT), Korea Advanced Institute of Science and Technology, Daejeon 305-701 (Republic of Korea).
authors.forename: Cheol Am
authors.initials: CA
authors.lastname: Hong
authors.affiliation: Department of Biological Engineering, The David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139 (USA).
authors.forename: Ahmed A
authors.initials: AA
authors.lastname: Eltoukhy
authors.affiliation: Graduate School of Pharmaceutical Sciences, College of Pharmacy, Ewha Womans University, Seoul (Republic of Korea).
authors.forename: Hyukjin
authors.initials: H
authors.lastname: Lee
authors.affiliation: Department of Biological Engineering, The David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139 (USA).
authors.forename: Robert
authors.initials: R
authors.lastname: Langer
authors.affiliation: Department of Biological Engineering, The David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139 (USA). dgander@mit.edu.
authors.forename: Daniel G
authors.initials: DG
authors.lastname: Anderson
authors.affiliation: Department of Biological Sciences, Department of Materials Science and Engineering, KI for NanoCentury (KINC CNiT), Korea Advanced Institute of Science and Technology, Daejeon 305-701 (Republic of Korea). yoonsung@kaist.ac.kr.
authors.forename: Yoon Sung
authors.initials: YS
authors.lastname: Nam
mesh_terms: D050091:Dendrimers / Q000737:chemistry*; D020868:Gene Silencing; D049329:Nanostructures / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D034741:RNA, Small Interfering / Q000737:chemistry* / Q000235:genetics*
chemical_list: D050091:Dendrimers; D011108:Polymers; D034741:RNA, Small Interfering; C507253:poly(beta-amino ester)
keywords: dendrimeric nanostructures; gene silencing; poly(β-amino ester); polymeric condensation; siRNA