title: [Detection of Angiostrongylus cantonensis larvae with a high resolution melt PCR assay].
abstract: OBJECTIVE
  To develop a detection method of the third-stage larvae of Angiostrongylus cantonensis by real-time PCR and high-resolution melt curve analysis.
  
  
  METHODS
  A pair of specific primers was designed based on the internal transcribed spacer 1 (ITS1) region of the nuclear ribosomal DNA of A. cantonensis. The third-stage larvae of A. cantonensis were detected by real-time PCR and high-resolution melt curve analysis. The specificity of the method was analyzed by testing DNAs of A. cantonensis, Clonorchis sinensis, and Gnathostoma spinigerum. The genomic DNA were extracted from 1 to 10 third-stage larvae of A. cantonensis, respectively, and used to identify the sensitivity of the method.
  
  
  RESULTS
  This method could specifically detect A. cantonensis and the detection limit reached to one larva. No amplification curve and melt curve were found in C. sinensis and G. spinigerum.
  
  
  CONCLUSIONS
  Real-time PCR and high-resolution melt curve analysis show good specificity and sensitivity for detecting the third-stage larvae of A. cantonensis.
authors.forename: Yan-kun
authors.initials: YK
authors.lastname: Ke
authors.forename: Yu
authors.initials: Y
authors.lastname: Huang
authors.forename: Xiu-qing
authors.initials: XQ
authors.lastname: Ge
authors.forename: Mei-xian
authors.initials: MX
authors.lastname: Peng
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.forename: Li-ren
authors.initials: LR
authors.lastname: Huang
authors.forename: Guo-qiang
authors.initials: GQ
authors.lastname: Luo
authors.forename: Lei-xiang
authors.initials: LX
authors.lastname: Zhong
authors.forename: Shi-fu
authors.initials: SF
authors.lastname: Kan
authors.forename: Yuan-xin
authors.initials: YX
authors.lastname: Zhang
authors.forename: Jian-xin
authors.initials: JX
authors.lastname: Zhai
mesh_terms: D017185:Angiostrongylus cantonensis; D000818:Animals; D017931:DNA Primers; D004275:DNA, Ribosomal; D007814:Larva; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D004275:DNA, Ribosomal