title: Polymorphonuclear leukocyte apoptosis is accelerated by sulfatides or sulfatides-treated Salmonella Typhimurium bacteria.
abstract: Neutrophils die by apoptosis following activation and uptake of microbes or enter apoptosis spontaneously at the end of their lifespan if they do not encounter a pathogen. Here we report that sulfatides or sulfatides-treated Salmonella Typhimurium bacteria accelerated human neutrophil apoptosis. Neutrophil apoptosis was examined by flow cytometry. Sulfatides caused prominent increase in percentage of apoptotic cells after 2.5 hrs of incubation. Salmonella Typhimurium bacteria by themselves did not affect the basal level of apoptosis in neutrophil population. When neutrophils were added to S. Typhimurium "opsonized" by sulfatides, apoptotic index significantly increased, whereas the number of phagocyting cells was not influenced. Sulfatides' proapoptotic effect was strongly dependent on the activity of β-galactosidase; inhibition of this enzyme impaired its potency to accelerate apoptosis. These data support the mechanism of neutrophil apoptosis triggering based on sulfatides' ability to accumulate in intracellular compartments and mediate successive increase in ceramide content resulting from β-galactosidase activity.
authors.affiliation: Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119234, Russia.
authors.forename: Zoryana V
authors.initials: ZV
authors.lastname: Grishina
authors.affiliation: Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119234, Russia.
authors.forename: Galina M
authors.initials: GM
authors.lastname: Viryasova
authors.affiliation: Gamaleya Research Institute of Epidemiology and Microbiology, Moscow 123098, Russia.
authors.forename: Yulia M
authors.initials: YM
authors.lastname: Romanova
authors.affiliation: Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119234, Russia.
authors.forename: Galina F
authors.initials: GF
authors.lastname: Sud'ina
mesh_terms: D017209:Apoptosis; D020169:Caspases / Q000378:metabolism; D002518:Ceramides / Q000737:chemistry; D005434:Flow Cytometry; D006801:Humans; D009504:Neutrophils / Q000473:pathology*; D009895:Opsonin Proteins / Q000378:metabolism; D010587:Phagocytosis; D012486:Salmonella typhimurium; D013433:Sulfoglycosphingolipids / Q000737:chemistry*; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D002518:Ceramides; D009895:Opsonin Proteins; D013433:Sulfoglycosphingolipids; D001616:beta-Galactosidase; D020169:Caspases