title: Direct Nuclear Delivery of DNA by Photothermal Nanoblade.
abstract: We demonstrate direct nuclear delivery of DNA into live mammalian cells using the photothermal nanoblade. Pulsed laser-triggered cavitation bubbles on a titanium-coated micropipette tip punctured both cellular plasma and nuclear membranes, which was followed by pressure-controlled delivery of DNA into the nucleus. High-level and efficient plasmid expression in different cell types with maintained cell viability was achieved.
authors.affiliation: Department of Mechanical and Aerospace Engineering, UCLA, Los Angeles, CA, USA Department of Pathology and Laboratory Medicine, UCLA, Los Angeles, CA, USA.
authors.forename: Ting-Hsiang
authors.initials: TH
authors.lastname: Wu
authors.affiliation: Department of Mechanical and Aerospace Engineering, UCLA, Los Angeles, CA, USA.
authors.forename: Yi-Chien
authors.initials: YC
authors.lastname: Wu
authors.affiliation: Department of Pathology and Laboratory Medicine, UCLA, Los Angeles, CA, USA.
authors.forename: Enrico
authors.initials: E
authors.lastname: Sagullo
authors.affiliation: Department of Pathology and Laboratory Medicine, UCLA, Los Angeles, CA, USA.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Teitell
authors.affiliation: Department of Mechanical and Aerospace Engineering, UCLA, Los Angeles, CA, USA pychiou@seas.ucla.edu.
authors.forename: Pei-Yu
authors.initials: PY
authors.lastname: Chiou
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D002470:Cell Survival; D004247:DNA / Q000235:genetics* / Q000378:metabolism*; D005057:Eukaryotic Cells; D015870:Gene Expression; D006801:Humans; D007834:Lasers; D008322:Mammals; D045423:Microbubbles; D008845:Microinjections / Q000379:methods*; D036103:Nanotechnology / Q000379:methods; D014162:Transfection / Q000379:methods*; D014170:Transformation, Genetic
chemical_list: D004247:DNA
keywords: lab-on-a-chip; microfluidics; microtechnology; nanobiotech; nanotechnology