title: A novel cyclic dipeptide from deep marine-derived fungus Aspergillus sp. SCSIOW2.
abstract: A novel cyclic dipeptide, 14-hydroxy-cyclopeptine (1), was purified from a deep sea derived fungal isolate identified as an Aspergillus sp. The structure was elucidated by detailed spectroscopic analyses using 1D and 2D NMR experiments and high resolution mass spectrometry. The absolute configuration of the amino acid was determined by Marfey's method. Two conformational isomers of 1 were established by ROE analyses. 1 inhibited nitric oxide production with IC50 values at 40.3 μg/mL in a lipopolysaccharide and recombinant mouse interferon-γ -activated macrophage-like cell line, RAW 264.7 and showed no cytotoxic effect in the tested dose range up to 100 μg/mL.
authors.affiliation: a College of Life Science, Shenzhen Key Laboratory of Marine Bioresourse and Eco-environmental Science, Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen University , Shenzhen 518060 , P.R. China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhou
authors.affiliation: a College of Life Science, Shenzhen Key Laboratory of Marine Bioresourse and Eco-environmental Science, Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen University , Shenzhen 518060 , P.R. China.
authors.forename: Pingyan
authors.initials: P
authors.lastname: Fang
authors.affiliation: a College of Life Science, Shenzhen Key Laboratory of Marine Bioresourse and Eco-environmental Science, Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen University , Shenzhen 518060 , P.R. China.
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Tang
authors.affiliation: a College of Life Science, Shenzhen Key Laboratory of Marine Bioresourse and Eco-environmental Science, Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen University , Shenzhen 518060 , P.R. China.
authors.forename: Zhiqin
authors.initials: Z
authors.lastname: Wu
authors.affiliation: a College of Life Science, Shenzhen Key Laboratory of Marine Bioresourse and Eco-environmental Science, Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen University , Shenzhen 518060 , P.R. China.
authors.forename: Xiaofan
authors.initials: X
authors.lastname: Li
authors.affiliation: a College of Life Science, Shenzhen Key Laboratory of Marine Bioresourse and Eco-environmental Science, Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen University , Shenzhen 518060 , P.R. China.
authors.forename: Shuiming
authors.initials: S
authors.lastname: Li
authors.affiliation: a College of Life Science, Shenzhen Key Laboratory of Marine Bioresourse and Eco-environmental Science, Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen University , Shenzhen 518060 , P.R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: a College of Life Science, Shenzhen Key Laboratory of Marine Bioresourse and Eco-environmental Science, Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen University , Shenzhen 518060 , P.R. China.
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.affiliation: b Department of Pharmacology , School of Medicine, Shenzhen University , Shenzhen 518060 , P.R. China.
authors.forename: Zhendan
authors.initials: Z
authors.lastname: He
authors.affiliation: a College of Life Science, Shenzhen Key Laboratory of Marine Bioresourse and Eco-environmental Science, Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen University , Shenzhen 518060 , P.R. China.
authors.forename: Deming
authors.initials: D
authors.lastname: Gou
authors.affiliation: c Institute of Traditional Chinese Medicine & Natural Products, Jinan University , Guangzhou 510632 , P.R. China.
authors.forename: Xinsheng
authors.initials: X
authors.lastname: Yao
authors.affiliation: a College of Life Science, Shenzhen Key Laboratory of Marine Bioresourse and Eco-environmental Science, Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen University , Shenzhen 518060 , P.R. China.
authors.forename: Liyan
authors.initials: L
authors.lastname: Wang
mesh_terms: D000818:Animals; D001230:Aspergillus / Q000737:chemistry* / Q000302:isolation & purification; D002460:Cell Line / Q000187:drug effects; D004151:Dipeptides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D020128:Inhibitory Concentration 50; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D010456:Peptides, Cyclic / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D011487:Protein Conformation; D012623:Seawater / Q000382:microbiology
chemical_list: C000609426:14-hydroxy-cyclopeptine; D004151:Dipeptides; D008070:Lipopolysaccharides; D010456:Peptides, Cyclic; D009569:Nitric Oxide
keywords: Aspergillus sp; cyclodipeptide; marine fungi; nitric oxide inhibitory activity