title: A label-free fluorescent molecular switch for a DNA hybridization assay utilizing a G-quadruplex-selective auramine O.
abstract: A sensitive and selective assay of DNA is developed by utilizing a signal transduction strategy with the rational redesign of the hairpin structured G-quadruplex molecular switch (G4-MS) assembled using auramine O (AO). By monitoring the changes of the fluorescent signal, we could identify and further quantitatively determine the target DNA in the samples.
authors.affiliation: Department of Chemistry, State Key Lab of Molecular Engineering of Polymers and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China. bhliu@fudan.edu.cn.
authors.forename: Huiying
authors.initials: H
authors.lastname: Xu
authors.forename: Fenghua
authors.initials: F
authors.lastname: Geng
authors.forename: Yongxiang
authors.initials: Y
authors.lastname: Wang
authors.forename: Maotian
authors.initials: M
authors.lastname: Xu
authors.forename: Xinhe
authors.initials: X
authors.lastname: Lai
authors.forename: Peng
authors.initials: P
authors.lastname: Qu
authors.forename: Yintang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Baohong
authors.initials: B
authors.lastname: Liu
mesh_terms: D001576:Benzophenoneidum / Q000737:chemistry*; D004247:DNA / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry*; D054856:G-Quadruplexes; D006801:Humans; D015394:Molecular Structure; D009693:Nucleic Acid Hybridization
chemical_list: D005456:Fluorescent Dyes; D001576:Benzophenoneidum; D004247:DNA