title: Stimulating the biosynthesis of antroquinonol by addition of effectors and soybean oil in submerged fermentation of Antrodia camphorata.
abstract: Antrodia camphorata is a precious medicinal mushroom that has attracted increasing attentions. Antroquinonol has been considered as being among the most biologically active components of A. camphorata. However, it was hardly biosynthesized via conventional submerged fermentation. Two approaches were applied to stimulate the biosynthesis of antroquinonol in submerged fermentation. On one hand, different kinds of effectors that may involve in the antroquinonol biosynthesis were investigated. Among the tested effectors, camphorwood leach liquor was the most effective for stimulating the antroquinonol production. On the other hand, because of the hydrophobic characteristics of antroquinonol, soybean oil was added to establish an extractive fermentation system for alleviating the product inhibition and resulting in enhanced productivity. The highest antroquinonol concentration could be achieved at 89.06 ± 0.14 mg/L when 10% (v/v) soybean oil was added at the beginning of the fermentation. This study will be of great significance for the study of A. camphorata and the bioprocess regulation of antroquinonol production.
authors.affiliation: Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, People's Republic of China.
authors.forename: Yong-Dan
authors.initials: YD
authors.lastname: Hu
authors.affiliation: Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, People's Republic of China.
authors.forename: Rui-Qiu
authors.initials: RQ
authors.lastname: Lu
authors.affiliation: Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, People's Republic of China.
authors.forename: Xiang-Ru
authors.initials: XR
authors.lastname: Liao
authors.affiliation: Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, People's Republic of China.
authors.forename: Bo-Bo
authors.initials: BB
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, People's Republic of China.
authors.forename: Gan-Rong
authors.initials: GR
authors.lastname: Xu
mesh_terms: D055491:Antrodia / Q000187:drug effects* / Q000378:metabolism*; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D027424:Cinnamomum / Q000737:chemistry; D003470:Culture Media / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D005285:Fermentation / Q000187:drug effects*; D007101:Immersion; D010936:Plant Extracts / Q000494:pharmacology*; D013024:Soybean Oil / Q000494:pharmacology*; D013997:Time Factors; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000096:biosynthesis
chemical_list: D003470:Culture Media; D010936:Plant Extracts; D014451:Ubiquinone; D013024:Soybean Oil; C545357:antroquinonol
keywords: Antrodia camphorata; antroquinonol; bioactive compound; mushroom; process regulation; submerged fermentation