title: Isolation and characterization of Flavobacterium columnare from freshwater ornamental goldfish Carassius auratus.
abstract: Filamentous bacteria overlaying ulcerated area on the body surface were observed in the wet-mout preparation from a moribund goldfish with saddle back appearance. The causative agent was identified as Flavobacterium columnrae, on the basis of biochemical test, species-specific polymerase chain reaction (PCR) and sequencing of 16S rDNA gene with the universal bacterial primers. Furthermore, the strain (ING-1) attributed to genomovar II in 16S rDNA PCR-restriction fragment length polymorphism (PCR-RFLP) and sequence analysis. In phylogenetic analysis, the strain ING-1, produced typical columnaris disease symptoms in rohu (Labeo rohita) fingerlings within 10 days. This is a new record about molecular detection and identification of Flavobacterium columnare, occurring naturally on a new host Carassius auratusin India.
authors.forename: Dev Kumar
authors.initials: DK
authors.lastname: Verma
authors.forename: Gaurav
authors.initials: G
authors.lastname: Rathore
authors.forename: Pravata Kumar
authors.initials: PK
authors.lastname: Pradhan
authors.forename: Neeraj
authors.initials: N
authors.lastname: Sood
authors.forename: Peyush
authors.initials: P
authors.lastname: Punia
mesh_terms: D000818:Animals; D005393:Fish Diseases / Q000382:microbiology*; D045826:Flavobacteriaceae Infections / Q000382:microbiology / Q000662:veterinary*; D005417:Flavobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D005618:Fresh Water; D006054:Goldfish; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S