title: Construction of a novel guest biomimetic glutathione peroxidase with solvent-dependent catalytic behavior by incorporating the active center into adamantyl molecule.
abstract: A novel guest biomimetic glutathione peroxidase (3,3'-tellurobis(propane-3,1-diyl) diadamantanecarboxylate, denoted as ADA-Te-ADA) was synthesized. ADA-Te-ADA functioned to overcome the disadvantages in the construction of building block for giant supramolecular biomimetic enzyme. To reveal the catalytic property of hydrophobic ADA-Te-ADA, the catalytic mechanism was investigated using PBS (phosphate buffer (pH 7.0. 50 mM))/methanol solvent mixture as assay solution. Itindicated that ADA-Te-ADA exhibited typical enzyme catalytic behavior by saturation kinetics measurement. Importantly, ADA-Te-ADA exhibited the typical solvent-dependent catalytic behavior. And the highest catalytic rate 4.29 µM x min-1 was obtained when the volume ratio of PBs: methanol was 5 : 5. Especially, the catalytic rates obtained based on various substrates proved that ADA-Te-ADA slightly displayed special substrate selectivity, which was the ideal catalytic characterization of building block for giant supramolecular biomimetic enzyme. The successfully synthesis of ADA-Te-ADA might highlight for the understanding of the catalytic mechanism of hydrophobic guest biomimetic glutathione peroxidase. And it also might provide the basement for the construction of giant supramolecular biomimetic enzyme.
authors.forename: Yanzhen
authors.initials: Y
authors.lastname: Yin
authors.forename: Chao
authors.initials: C
authors.lastname: Lang
authors.forename: Xiaoxi
authors.initials: X
authors.lastname: Hua
authors.forename: Zhongfeng
authors.initials: Z
authors.lastname: Shia
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.forename: Shufei
authors.initials: S
authors.lastname: Jiao
authors.forename: Chengxiang
authors.initials: C
authors.lastname: Cai
authors.forename: Junqiu
authors.initials: J
authors.lastname: Liu
mesh_terms: D000218:Adamantane / Q000138:chemical synthesis* / Q000737:chemistry; D055162:Biocatalysis; D032701:Biomimetics; D020134:Catalytic Domain; D005979:Glutathione Peroxidase / Q000138:chemical synthesis* / Q000737:chemistry; D007700:Kinetics; D019906:Nuclear Magnetic Resonance, Biomolecular; D012997:Solvents / Q000737:chemistry
chemical_list: D012997:Solvents; D005979:Glutathione Peroxidase; D000218:Adamantane