title: In vitro RNA uptake studies in plant mitochondria.
abstract: During evolution, most of the ancestral genes from the endosymbiotic α-proteobacteria at the origin of mitochondria have been either lost or transferred to the nuclear genome. To allow the comeback of proteins and RNAs [in particular transfer RNA (tRNAs)] into the organelle, macromolecule import systems were universally established. While protein import processes have been studied into details, much less is known about tRNA mitochondrial import. In plants, part of the knowledge on the tRNA import process into mitochondria has been acquired thanks to in vitro import assays. Furthermore, the development of in vitro RNA import strategies allowed the study of plant mitochondrial gene expression. The purpose of this chapter is to provide detailed protocols to perform in vitro RNA uptake into potato (Solanum tuberosum) or Arabidopsis (Arabidopsis thaliana) mitochondria as well as approaches to analyze them.
authors.affiliation: Australian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009, Australia.
authors.forename: Szymon
authors.initials: S
authors.lastname: Kubiszewski-Jakubiak
authors.forename: Cyrille
authors.initials: C
authors.lastname: Megel
authors.forename: Elodie
authors.initials: E
authors.lastname: Ubrig
authors.forename: Thalia
authors.initials: T
authors.lastname: Salinas
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Duchêne
authors.forename: Laurence
authors.initials: L
authors.lastname: Maréchal-Drouard
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism*; D034443:RNA Transport; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism*; D012343:RNA, Transfer / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D011198:Solanum tuberosum / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D018749:RNA, Plant; D012343:RNA, Transfer