title: Structure of the human 80S ribosome.
abstract: Ribosomes are translational machineries that catalyse protein synthesis. Ribosome structures from various species are known at the atomic level, but obtaining the structure of the human ribosome has remained a challenge; efforts to address this would be highly relevant with regard to human diseases. Here we report the near-atomic structure of the human ribosome derived from high-resolution single-particle cryo-electron microscopy and atomic model building. The structure has an average resolution of 3.6 Å, reaching 2.9 Å resolution in the most stable regions. It provides unprecedented insights into ribosomal RNA entities and amino acid side chains, notably of the transfer RNA binding sites and specific molecular interactions with the exit site tRNA. It reveals atomic details of the subunit interface, which is seen to remodel strongly upon rotational movements of the ribosomal subunits. Furthermore, the structure paves the way for analysing antibiotic side effects and diseases associated with deregulated protein synthesis.
authors.affiliation: 1] Centre for Integrative Biology (CBI), Department of Integrated Structural Biology, IGBMC (Institute of Genetics and of Molecular and Cellular Biology), 1 rue Laurent Fries, 67404 Illkirch, France [2] Centre National de la Recherche Scientifique (CNRS), UMR 7104, 67404 Illkirch, France [3] Institut National de la Santé et de la Recherche Médicale (INSERM) U964, 67404 Illkirch, France [4] Université de Strasbourg, 67081 Strasbourg, France.
authors.forename: Heena
authors.initials: H
authors.lastname: Khatter
authors.affiliation: 1] Centre for Integrative Biology (CBI), Department of Integrated Structural Biology, IGBMC (Institute of Genetics and of Molecular and Cellular Biology), 1 rue Laurent Fries, 67404 Illkirch, France [2] Centre National de la Recherche Scientifique (CNRS), UMR 7104, 67404 Illkirch, France [3] Institut National de la Santé et de la Recherche Médicale (INSERM) U964, 67404 Illkirch, France [4] Université de Strasbourg, 67081 Strasbourg, France.
authors.forename: Alexander G
authors.initials: AG
authors.lastname: Myasnikov
authors.affiliation: 1] Centre for Integrative Biology (CBI), Department of Integrated Structural Biology, IGBMC (Institute of Genetics and of Molecular and Cellular Biology), 1 rue Laurent Fries, 67404 Illkirch, France [2] Centre National de la Recherche Scientifique (CNRS), UMR 7104, 67404 Illkirch, France [3] Institut National de la Santé et de la Recherche Médicale (INSERM) U964, 67404 Illkirch, France [4] Université de Strasbourg, 67081 Strasbourg, France.
authors.forename: S Kundhavai
authors.initials: SK
authors.lastname: Natchiar
authors.affiliation: 1] Centre for Integrative Biology (CBI), Department of Integrated Structural Biology, IGBMC (Institute of Genetics and of Molecular and Cellular Biology), 1 rue Laurent Fries, 67404 Illkirch, France [2] Centre National de la Recherche Scientifique (CNRS), UMR 7104, 67404 Illkirch, France [3] Institut National de la Santé et de la Recherche Médicale (INSERM) U964, 67404 Illkirch, France [4] Université de Strasbourg, 67081 Strasbourg, France.
authors.forename: Bruno P
authors.initials: BP
authors.lastname: Klaholz
mesh_terms: D001665:Binding Sites; D020285:Cryoelectron Microscopy; D004583:Electrons; D006801:Humans; D008958:Models, Molecular; D012335:RNA, Ribosomal / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D012343:RNA, Transfer / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D012269:Ribosomal Proteins / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D054657:Ribosome Subunits / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D012270:Ribosomes / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure*
chemical_list: D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; D012343:RNA, Transfer