title: Generation and Characterization of Transgenic Mice Expressing Mouse Ins1 Promoter for Pancreatic β-Cell-Specific Gene Overexpression and Knockout.
abstract: The technologies for pancreatic β-cell-specific gene overexpression or knockout are fundamental for investigations of functional genes in vivo. Here we generated the Ins1-Cre-Dsred and Ins1-rtTA mouse models, which expressed the Cre recombinase or reverse tetracycline regulatable transactivator (rtTA) without hGH minigene under the control of mouse Ins1 promoter. Our data showed that the Cre-mediated recombination and rtTA-mediated activation could be efficiently detected at embryonic day 13.5 when these models were crossed with the reporter mice (ROSA(mT/mG) or tetO-HIST1H2BJ/GFP). The Cre and rtTA expression was restricted to β-cells without leakage in the brain and other tissues. Moreover, both the transgenic lines showed normal glucose tolerance and insulin secretion. These results suggested that the Ins1-Cre-Dsred and Ins1-rtTA mice could be used to knock out or overexpress target genes in embryos and adults to facilitate β-cell researches.
authors.affiliation: Laboratory of Endocrinology and Metabolism (Y.C., G.N.), Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences and Shanghai Jiao Tong University School of Medicine (Y.C., G.N.), and Shanghai Clinical Center for Endocrine and Metabolic Diseases (Y.S., A.S., X.J., Q.M., W.W., G.N., Y.C.), Shanghai Key Laboratory for Endocrine Tumors, Rui-Jin Hospital, Shanghai Jiao-Tong University School of Medicine, Shanghai 200025, China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Laboratory of Endocrinology and Metabolism (Y.C., G.N.), Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences and Shanghai Jiao Tong University School of Medicine (Y.C., G.N.), and Shanghai Clinical Center for Endocrine and Metabolic Diseases (Y.S., A.S., X.J., Q.M., W.W., G.N., Y.C.), Shanghai Key Laboratory for Endocrine Tumors, Rui-Jin Hospital, Shanghai Jiao-Tong University School of Medicine, Shanghai 200025, China.
authors.forename: Yutong
authors.initials: Y
authors.lastname: Su
authors.affiliation: Laboratory of Endocrinology and Metabolism (Y.C., G.N.), Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences and Shanghai Jiao Tong University School of Medicine (Y.C., G.N.), and Shanghai Clinical Center for Endocrine and Metabolic Diseases (Y.S., A.S., X.J., Q.M., W.W., G.N., Y.C.), Shanghai Key Laboratory for Endocrine Tumors, Rui-Jin Hospital, Shanghai Jiao-Tong University School of Medicine, Shanghai 200025, China.
authors.forename: Aijing
authors.initials: A
authors.lastname: Shan
authors.affiliation: Laboratory of Endocrinology and Metabolism (Y.C., G.N.), Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences and Shanghai Jiao Tong University School of Medicine (Y.C., G.N.), and Shanghai Clinical Center for Endocrine and Metabolic Diseases (Y.S., A.S., X.J., Q.M., W.W., G.N., Y.C.), Shanghai Key Laboratory for Endocrine Tumors, Rui-Jin Hospital, Shanghai Jiao-Tong University School of Medicine, Shanghai 200025, China.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Laboratory of Endocrinology and Metabolism (Y.C., G.N.), Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences and Shanghai Jiao Tong University School of Medicine (Y.C., G.N.), and Shanghai Clinical Center for Endocrine and Metabolic Diseases (Y.S., A.S., X.J., Q.M., W.W., G.N., Y.C.), Shanghai Key Laboratory for Endocrine Tumors, Rui-Jin Hospital, Shanghai Jiao-Tong University School of Medicine, Shanghai 200025, China.
authors.forename: Qinyun
authors.initials: Q
authors.lastname: Ma
authors.affiliation: Laboratory of Endocrinology and Metabolism (Y.C., G.N.), Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences and Shanghai Jiao Tong University School of Medicine (Y.C., G.N.), and Shanghai Clinical Center for Endocrine and Metabolic Diseases (Y.S., A.S., X.J., Q.M., W.W., G.N., Y.C.), Shanghai Key Laboratory for Endocrine Tumors, Rui-Jin Hospital, Shanghai Jiao-Tong University School of Medicine, Shanghai 200025, China.
authors.forename: Weiqing
authors.initials: W
authors.lastname: Wang
authors.affiliation: Laboratory of Endocrinology and Metabolism (Y.C., G.N.), Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences and Shanghai Jiao Tong University School of Medicine (Y.C., G.N.), and Shanghai Clinical Center for Endocrine and Metabolic Diseases (Y.S., A.S., X.J., Q.M., W.W., G.N., Y.C.), Shanghai Key Laboratory for Endocrine Tumors, Rui-Jin Hospital, Shanghai Jiao-Tong University School of Medicine, Shanghai 200025, China.
authors.forename: Guang
authors.initials: G
authors.lastname: Ning
authors.affiliation: Laboratory of Endocrinology and Metabolism (Y.C., G.N.), Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences and Shanghai Jiao Tong University School of Medicine (Y.C., G.N.), and Shanghai Clinical Center for Endocrine and Metabolic Diseases (Y.S., A.S., X.J., Q.M., W.W., G.N., Y.C.), Shanghai Key Laboratory for Endocrine Tumors, Rui-Jin Hospital, Shanghai Jiao-Tong University School of Medicine, Shanghai 200025, China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Cao
mesh_terms: D000818:Animals; D015870:Gene Expression / Q000235:genetics; D055879:Gene Knock-In Techniques / Q000379:methods*; D007328:Insulin / Q000235:genetics*; D050417:Insulin-Secreting Cells / Q000378:metabolism*; D019426:Integrases / Q000235:genetics; D008164:Luminescent Proteins / Q000235:genetics; D051379:Mice; D018345:Mice, Knockout / Q000235:genetics*; D008822:Mice, Transgenic / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics
chemical_list: C514817:Ins1 protein, mouse; D007328:Insulin; D008164:Luminescent Proteins; C484898:Men1 protein, mouse; D011518:Proto-Oncogene Proteins; D015534:Trans-Activators; C413663:fluorescent protein 583; C045073:Cre recombinase; D019426:Integrases