title: Sensitive electrochemical immunosensor for α-fetoprotein based on graphene/SnO2/Au nanocomposite.
abstract: A label-free electrochemical immunosensor for sensitive detection of α-fetoprotein (AFP) was developed based on graphene/SnO2/Au nanocomposite. The graphene/SnO2/Au nanocomposite modified glassy carbon electrode was used to immobilize α-fetoprotein antibody (anti-AFP) and to construct the immunosensor. Results demonstrated that the peak currents of [Ru(NH3)6](3+) decreased due to the interaction between antibody and antigen on the modified electrode. Thus, a label-free immunosensor for the detection of AFP was realized by monitoring the peak current change of [Ru(NH3)6](3+). The factors influencing the performance of the immunosensor were investigated in details. Under optimal conditions, the peak currents obtained by DPV decreased linearly with the increasing AFP concentrations in the range from 0.02 to 50 ng mL(-1) with a linear coefficient of 0.9959. This electrochemical immunoassay has a low detection limit of 0.01 ng mL(-1) (S/N=3) and was successfully applied to the determination of AFP in serum samples.
authors.affiliation: School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences, 19A YuQuan Road, Beijing 100049, China.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Liu
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Colloids and Surfaces, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Guanhua
authors.initials: G
authors.lastname: Lin
authors.affiliation: School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences, 19A YuQuan Road, Beijing 100049, China.
authors.forename: Can
authors.initials: C
authors.lastname: Xiao
authors.affiliation: School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences, 19A YuQuan Road, Beijing 100049, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xue
authors.affiliation: School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences, 19A YuQuan Road, Beijing 100049, China.
authors.forename: Ankang
authors.initials: A
authors.lastname: Yang
authors.affiliation: National Center for Nanoscience and Technology of China, Beijing 100080, China. Electronic address: bruseren@nanoctr.cn.
authors.forename: Hongxuan
authors.initials: H
authors.lastname: Ren
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Colloids and Surfaces, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China. Electronic address: luwensheng@iccas.ac.cn.
authors.forename: Wensheng
authors.initials: W
authors.lastname: Lu
authors.affiliation: School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences, 19A YuQuan Road, Beijing 100049, China. Electronic address: hongzhao@ucas.ac.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhao
authors.affiliation: School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences, 19A YuQuan Road, Beijing 100049, China.
authors.forename: Xiangjun
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences, 19A YuQuan Road, Beijing 100049, China.
authors.forename: Zhuobin
authors.initials: Z
authors.lastname: Yuan
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D055664:Electrochemical Techniques / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D017971:Tin Compounds / Q000737:chemistry*; D000509:alpha-Fetoproteins / Q000032:analysis*
chemical_list: D017971:Tin Compounds; D000509:alpha-Fetoproteins; D006046:Gold; D006108:Graphite; C045358:stannic oxide
keywords: Electrochemical immunosensor; Graphene; Nanocomposite; α-Fetoprotein