title: Pathological features of proteinuric nephropathy resembling Alport syndrome in a young Pyrenean Mountain dog.
abstract: The renal biopsy tissue from a 9-month-old, male Pyrenean Mountain dog with renal disorder and severe proteinuria was examined. Ultrastructural examination revealed multilaminar splitting and fragmentation of the glomerular basement membrane (GBM) and diffuse podocyte foot process effacement. Immunofluorescent staining for α(IV) chains revealed presence of α5(IV) and complete absence of α3(IV) and α4(IV) chains in the GBM. Immunohistochemistry also revealed decreased and altered expression of nephrin and podocin in the glomeruli compared with normal canine glomeruli. These results suggested that the glomerular disease of the present case might be consistent with canine hereditary nephropathy resembling human Alport syndrome caused by genetic defect of type IV collagen, and indicated possible contribution of podocyte injury to severe proteinuria in this case.
authors.affiliation: Laboratory of Veterinary Pathology, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252-5201, Japan.
authors.forename: Go
authors.initials: G
authors.lastname: Sugahara
authors.forename: Ichiro
authors.initials: I
authors.lastname: Naito
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Miyagawa
authors.forename: Takaaki
authors.initials: T
authors.lastname: Komiyama
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Takemura
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Kobayashi
authors.forename: Takayuki
authors.initials: T
authors.lastname: Mineshige
authors.forename: Junichi
authors.initials: J
authors.lastname: Kamiie
authors.forename: Kinji
authors.initials: K
authors.lastname: Shirota
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000473:pathology* / Q000652:urine; D004285:Dogs; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D050533:Glomerular Basement Membrane / Q000648:ultrastructure; D007668:Kidney / Q000473:pathology; D007674:Kidney Diseases / Q000473:pathology / Q000662:veterinary*; D008297:Male; D009394:Nephritis, Hereditary / Q000473:pathology / Q000662:veterinary*; D011507:Proteinuria / Q000473:pathology / Q000662:veterinary*