title: Effect of ciprofloxacin in the ultrastructure and development of biofilms formed by rapidly growing mycobacteria.
abstract: BACKGROUND
  The aim of this study was to analyze the effect of ciprofloxacin at different times on the development and behavior of intrinsic autofluorescence, covered area, thickness and cell viability in a biofilm formed by non-pigmented rapidly growing mycobacteria (NPRGM).Confocal laser scanning microscopy and image analysis were used to study the behavior of ciprofloxacin on biofilms.
  
  
  RESULTS
  Thickness was the most affected parameter, although some species showed changes in other parameters. At the same time, we also measured the minimum inhibitory concentration and the minimum biofilm eradication concentration (MBEC). An increase in MBEC was observed in all the strains, M. peregrinum being the species that presented the highest increase.
  
  
  CONCLUSIONS
  This study help us to understand better how mycobacterial biofims can be affected by ciprofloxacin.
authors.affiliation: Department of Clinical Microbiology, IIS- Fundación Jiménez Díaz. Av. Reyes Católicos 2, 28040, Madrid, Spain. mcme86@gmail.com.
authors.forename: María-Carmen
authors.initials: MC
authors.lastname: Muñoz-Egea
authors.affiliation: Department of Clinical Microbiology, IIS- Fundación Jiménez Díaz. Av. Reyes Católicos 2, 28040, Madrid, Spain. mgpedrazuela@gmail.com.
authors.forename: María
authors.initials: M
authors.lastname: García-Pedrazuela
authors.affiliation: Department of Epidemiology, IIS- Fundación Jiménez Díaz Av. Reyes Católicos 2, 28040, Madrid, Spain. IMahillo@fjd.es.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Mahillo
authors.affiliation: Department of Clinical Microbiology, IIS- Fundación Jiménez Díaz. Av. Reyes Católicos 2, 28040, Madrid, Spain. jestebanmoreno@gmail.com.
authors.forename: Jaime
authors.initials: J
authors.lastname: Esteban
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development*; D002939:Ciprofloxacin / Q000494:pharmacology*; D007091:Image Processing, Computer-Assisted; D008826:Microbial Sensitivity Tests; D018613:Microscopy, Confocal; D009170:Nontuberculous Mycobacteria / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology*
chemical_list: D000900:Anti-Bacterial Agents; D002939:Ciprofloxacin