title: [mRNA expression of Follistatin and follistatin-like 3, bone morphogenetic protein-4 antagonists in lung tissues of hypoxic mice].
abstract: OBJECTIVE
  To observe the expression changes of Follistatin (FSN) and follistatin-like 3 (FSRP) mRNA, both of bone morphogenetic protein-4(BMP4) antagonists, in mice lung tissue under different hypoxic time and its relation with BMP4.
  
  
  METHODS
  Thirty BMP4+/+ C57BL/6J wild type mice were randomly divided into normoxic control group, and 4 groups including 1 day, 3 days, 7 days and 21 days under hypoxic condition. The hypoxic animal model was established by exposing the mice to hypoxic condition for different time. The expressing level of FSN and FSRP mRNA in lung tissues were measured by Quantitative Real-Time PCR.
  
  
  RESULTS
  FSN and FSRP mRNA increased in hypoxic 1 day group as (28.0 ± 9.4) and (2.0 ± 0.4), in hypoxic 3 day group, FSN mRNA increased by (6.3 ± 3.2) and FSRP mRNA by (1.67 ± 0.7) (P < 0. 05). Compared with the normoxic group (1.77 ± 0.36) and (1.22 ± 0.15) (P < 0. 01), FSN and FSRP mRNA up-regulated in hypoxic 7 day group. The positive degree of FSN and FSRP mRNA in hypoxic 21 day group were (1.04 ± 0.27) and (0.85 ± 0.10) (P < 0. 05). In normoxic 1 day group, FSN mRNA of lung tissues of BMP4+/- C57BL/6J mice was (0.95 ± 0.05); FSRP mRNA was (1.11 ± 0.03) (P < 0. 05). In BMP4+/- C57BL/6J mice lung tissues, FSN mRNA were (1.10 ± 0.40) (P < 0. 05); FSRP mRNA were (0.85 ± 0.18).
  
  
  CONCLUSIONS
  The expression of FSN and FSRP mRNA in hypoxic 1;3;7 day mice lung tissues increased obviously, and FSN may play an important role in the hypoxic pulmonary hypertension through BMP4.
authors.affiliation: Guangzhou Institute of Respiratory Diseases, State Key Laboratory of Respiratory Diseases, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou 510120, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Li
authors.affiliation: Guangzhou Institute of Respiratory Diseases, State Key Laboratory of Respiratory Diseases, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou 510120, China.
authors.forename: Pixin
authors.initials: P
authors.lastname: Ran
authors.affiliation: Guangzhou Institute of Respiratory Diseases, State Key Laboratory of Respiratory Diseases, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou 510120, China. Email: jianwang1986@yahoo.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D055415:Bone Morphogenetic Protein 4; D038681:Follistatin / Q000096:biosynthesis*; D038702:Follistatin-Related Proteins / Q000096:biosynthesis*; D006976:Hypertension, Pulmonary / Q000378:metabolism*; D000860:Hypoxia; D008168:Lung; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger; D015854:Up-Regulation
chemical_list: D055415:Bone Morphogenetic Protein 4; D038681:Follistatin; D038702:Follistatin-Related Proteins; D012333:RNA, Messenger