title: Saccharification and ethanol fermentation from cholinium ionic liquid-pretreated bagasse with a different number of post-pretreatment washings.
abstract: Choline acetate (ChOAc), a cholinium ionic liquid (IL), was compared with 1-ethyl-3-methylimidazolium acetate (EmimOAc) with regard to biomass pretreatment, inhibition on cellulase and yeast, residuals in pretreated biomass, and saccharification and fermentation of pretreated biomass. Irrespective of ChOAc and EmimOAc, cellulose and hemicellulose saccharification of the IL-pretreated bagasse were over 90% and 60%, respectively. Median effective concentrations (EC50) based on cellulase activity were 32 wt% and 16 wt% for ChOAc and EmimOAc, respectively. The EC50 based on yeast growth were 3.1 wt% and 0.3 wt% for ChOAc and EmimOAc respectively. The residuals in IL-pretreated bagasse were 10% and 23% for ChOAc and EmimOAc, respectively, when washed 2 times after pretreatment. Ethanol yield on a bagasse basis were 60% and 24% for ChOAc and EmimOAc, respectively, in the saccharification and fermentation of IL-pretreated bagasse when washed 2 times. ChOAc-pretreated bagasse could be saccharified and fermented with fewer wash times than EmimOAc-pretreated bagasse.
authors.affiliation: Institute of Nature and Environmental Technology, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Ninomiya
authors.affiliation: Faculty of Natural System, Institute of Science and Engineering, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
authors.forename: Sayuri
authors.initials: S
authors.lastname: Omote
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.
authors.forename: Chiaki
authors.initials: C
authors.lastname: Ogino
authors.affiliation: Faculty of Natural System, Institute of Science and Engineering, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Kuroda
authors.affiliation: Faculty of Natural System, Institute of Science and Engineering, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
authors.forename: Mana
authors.initials: M
authors.lastname: Noguchi
authors.affiliation: Faculty of Natural System, Institute of Science and Engineering, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
authors.forename: Takatsugu
authors.initials: T
authors.lastname: Endo
authors.affiliation: Faculty of Natural System, Institute of Science and Engineering, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
authors.forename: Ryohei
authors.initials: R
authors.lastname: Kakuchi
authors.affiliation: Institute of Nature and Environmental Technology, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Shimizu
authors.affiliation: Faculty of Natural System, Institute of Science and Engineering, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan. Electronic address: ktkenji@staff.kanazawa-u.ac.jp.
authors.forename: Kenji
authors.initials: K
authors.lastname: Takahashi
mesh_terms: D018533:Biomass; D050260:Carbohydrate Metabolism / Q000187:drug effects*; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000737:chemistry* / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects*; D007093:Imidazoles / Q000494:pharmacology*; D052578:Ionic Liquids / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D014865:Waste Disposal, Fluid / Q000379:methods*
chemical_list: D007093:Imidazoles; D052578:Ionic Liquids; D000431:Ethanol; D002482:Cellulose; C027433:bagasse; D002480:Cellulase; C518739:1-ethyl-3-methylimidazolium
keywords: Cholinium; Ionic liquid; Lignocellulose; Pretreatment; Wash times