title: De Novo Assembly and Transcriptome Analysis of Wheat with Male Sterility Induced by the Chemical Hybridizing Agent SQ-1.
abstract: Wheat (Triticum aestivum L.), one of the world's most important food crops, is a strictly autogamous (self-pollinating) species with exclusively perfect flowers. Male sterility induced by chemical hybridizing agents has increasingly attracted attention as a tool for hybrid seed production in wheat; however, the molecular mechanisms of male sterility induced by the agent SQ-1 remain poorly understood due to limited whole transcriptome data. Therefore, a comparative analysis of wheat anther transcriptomes for male fertile wheat and SQ-1-induced male sterile wheat was carried out using next-generation sequencing technology. In all, 42,634,123 sequence reads were generated and were assembled into 82,356 high-quality unigenes with an average length of 724 bp. Of these, 1,088 unigenes were significantly differentially expressed in the fertile and sterile wheat anthers, including 643 up-regulated unigenes and 445 down-regulated unigenes. The differentially expressed unigenes with functional annotations were mapped onto 60 pathways using the Kyoto Encyclopedia of Genes and Genomes database. They were mainly involved in coding for the components of ribosomes, photosynthesis, respiration, purine and pyrimidine metabolism, amino acid metabolism, glutathione metabolism, RNA transport and signal transduction, reactive oxygen species metabolism, mRNA surveillance pathways, protein processing in the endoplasmic reticulum, protein export, and ubiquitin-mediated proteolysis. This study is the first to provide a systematic overview comparing wheat anther transcriptomes of male fertile wheat with those of SQ-1-induced male sterile wheat and is a valuable source of data for future research in SQ-1-induced wheat male sterility.
authors.affiliation: National Yangling Agricultural Biotechnology & Breeding Center, Yangling Branch of State Wheat Improvement Centre, Wheat Breeding Engineering Research Center, Ministry of Education, Key Laboratory of Crop Heterosis of Shaanxi Province, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Qidi
authors.initials: Q
authors.lastname: Zhu
authors.affiliation: National Yangling Agricultural Biotechnology & Breeding Center, Yangling Branch of State Wheat Improvement Centre, Wheat Breeding Engineering Research Center, Ministry of Education, Key Laboratory of Crop Heterosis of Shaanxi Province, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Song
authors.affiliation: National Yangling Agricultural Biotechnology & Breeding Center, Yangling Branch of State Wheat Improvement Centre, Wheat Breeding Engineering Research Center, Ministry of Education, Key Laboratory of Crop Heterosis of Shaanxi Province, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Gaisheng
authors.initials: G
authors.lastname: Zhang
authors.affiliation: National Yangling Agricultural Biotechnology & Breeding Center, Yangling Branch of State Wheat Improvement Centre, Wheat Breeding Engineering Research Center, Ministry of Education, Key Laboratory of Crop Heterosis of Shaanxi Province, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Ju
authors.affiliation: National Yangling Agricultural Biotechnology & Breeding Center, Yangling Branch of State Wheat Improvement Centre, Wheat Breeding Engineering Research Center, Ministry of Education, Key Laboratory of Crop Heterosis of Shaanxi Province, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Zhang
authors.affiliation: National Yangling Agricultural Biotechnology & Breeding Center, Yangling Branch of State Wheat Improvement Centre, Wheat Breeding Engineering Research Center, Ministry of Education, Key Laboratory of Crop Heterosis of Shaanxi Province, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Yongang
authors.initials: Y
authors.lastname: Yu
authors.affiliation: National Yangling Agricultural Biotechnology & Breeding Center, Yangling Branch of State Wheat Improvement Centre, Wheat Breeding Engineering Research Center, Ministry of Education, Key Laboratory of Crop Heterosis of Shaanxi Province, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Na
authors.initials: N
authors.lastname: Niu
authors.affiliation: National Yangling Agricultural Biotechnology & Breeding Center, Yangling Branch of State Wheat Improvement Centre, Wheat Breeding Engineering Research Center, Ministry of Education, Key Laboratory of Crop Heterosis of Shaanxi Province, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Junwei
authors.initials: J
authors.lastname: Wang
authors.affiliation: National Yangling Agricultural Biotechnology & Breeding Center, Yangling Branch of State Wheat Improvement Centre, Wheat Breeding Engineering Research Center, Ministry of Education, Key Laboratory of Crop Heterosis of Shaanxi Province, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Shoucai
authors.initials: S
authors.lastname: Ma
mesh_terms: D035264:Flowers / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D063990:Gene Ontology; D017343:Genes, Plant; D006824:Hybridization, Genetic / Q000187:drug effects; D016366:Open Reading Frames; D051479:Plant Infertility; D011724:Pyridazines / Q000494:pharmacology*; D059467:Transcriptome; D014908:Triticum / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism
chemical_list: D011724:Pyridazines