title: Polycomb repressive complex 2 epigenomic signature defines age-associated hypermethylation and gene expression changes.
abstract: Although age-associated gene expression and methylation changes have been reported throughout the literature, the unifying epigenomic principles of aging remain poorly understood. Recent explosion in availability and resolution of functional/regulatory genome annotation data (epigenomic data), such as that provided by the ENCODE and Roadmap Epigenomics projects, provides an opportunity for the identification of epigenomic mechanisms potentially altered by age-associated differentially methylated regions (aDMRs) and regulatory signatures in the promoters of age-associated genes (aGENs). In this study we found that aDMRs and aGENs identified in multiple independent studies share a common Polycomb Repressive Complex 2 signature marked by EZH2, SUZ12, CTCF binding sites, repressive H3K27me3, and activating H3K4me1 histone modification marks, and a "poised promoter" chromatin state. This signature is depleted in RNA Polymerase II-associated transcription factor binding sites, activating H3K79me2, H3K36me3, H3K27ac marks, and an "active promoter" chromatin state. The PRC2 signature was shown to be generally stable across cell types. When considering the directionality of methylation changes, we found the PRC2 signature to be associated with aDMRs hypermethylated with age, while hypomethylated aDMRs were associated with enhancers. In contrast, aGENs were associated with the PRC2 signature independently of the directionality of gene expression changes. In this study we demonstrate that the PRC2 signature is the common epigenomic context of genomic regions associated with hypermethylation and gene expression changes in aging.
authors.affiliation: a Department of Biostatistics; Virginia Commonwealth University ; Richmond , VA , USA.
authors.forename: Mikhail G
authors.initials: MG
authors.lastname: Dozmorov
mesh_terms: D000375:Aging / Q000235:genetics* / Q000473:pathology; D001665:Binding Sites; D000076246:CCCTC-Binding Factor; D002460:Cell Line; D002843:Chromatin / Q000235:genetics; D019175:DNA Methylation / Q000235:genetics*; D000071221:Enhancer of Zeste Homolog 2 Protein; D057890:Epigenomics; D005786:Gene Expression Regulation; D006657:Histones / Q000235:genetics; D006801:Humans; D009363:Neoplasm Proteins; D063151:Polycomb Repressive Complex 2 / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012097:Repressor Proteins / Q000235:genetics; D014157:Transcription Factors
chemical_list: D000076246:CCCTC-Binding Factor; C000619697:CTCF protein, human; D002843:Chromatin; D006657:Histones; D009363:Neoplasm Proteins; D012097:Repressor Proteins; C430461:SUZ12 protein, human; D014157:Transcription Factors; C498409:EZH2 protein, human; D000071221:Enhancer of Zeste Homolog 2 Protein; D063151:Polycomb Repressive Complex 2
keywords: ENCODE; ENCODE, Encyclopedia of DNA elements; GenomeRunner; PRC2; PRC2, Polycomb repressive complex 2; TFBS, transcription factor binding site; aDMR, age-associated differentially methylated region; aGEN, promoter of an age-associated gene; aging; epigenetics; epigenomics; methylation; polycomb