title: Molecular mechanisms of luteolin induced growth inhibition and apoptosis of human osteosarcoma cells.
abstract: Luteolin is a flavone in medicinal plants as well as some vegetables and spices. It is a natural anti-oxidant with less pro-oxidant potential but apparently with a better safety profile. The purpose of this study was to investigate the molecular mechanism of luteolin-mediated apoptosis of MG-63 human osteosarcoma cells. MTT assay kit was employed to evaluate the effects of luteolin on MG-63 cells proliferation. Then, we performed Annexin V-FITC/PI to analyze the apoptotic rate of the cells. Furthermore, the inhibitory effects of luteolin on the expressions of BCL-2, BAX, Caspase-3 and Survivin were detected by Western blotting. As expected, luteolin (0.5, 2.5, 12.5 µg/mL) inhibited the growth of MG-63 cells by inhibiting cell proliferation and inducing cell apoptosis. Western blotting demonstrated that luteolin (0.5, 2.5, 12.5 µg/mL) inhibited the expressions of BCL-2, Caspase-3 and Survivin, and promoted the expression of BAX in MG-63 cells with a concentration dependent way. Luteolin can inhibit osteosarcoma cell proliferation and induce apoptosis effectively in a dose dependent manner through down-regulating the expression of BCL-2, Caspase-3 and Survivin proteins levels and up-regulating the expression of BAX protein level. These findings indicated that luteolin may be used as a novel herbal medicine for the treatment of osteosarcoma.
authors.affiliation: Department of Orthopedics, 86 Hospital of PLA, Dangtu, Anhui 243100, China.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, Japan Union Hospital of Jilin University, Changchun130033,China.
authors.forename: Daliang
authors.initials: D
authors.lastname: Kong
authors.affiliation: Department of Orthopedics, 86 Hospital of PLA, Dangtu, Anhui 243100, China.
authors.forename: Xinwei
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, 86 Hospital of PLA, Dangtu, Anhui 243100, China.
authors.forename: Xiaoxiong
authors.initials: X
authors.lastname: Dong
authors.affiliation: Department of Pharmacy, Jinling Hospital, Nanjing, 210002, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Tao
authors.affiliation: Department of Orthopedics, 86 Hospital of PLA, Dangtu, Anhui 243100, China. ; Department of Orthopedics, Jingdu Hospital, Nanjing 210002, China.
authors.forename: Haiyang
authors.initials: H
authors.lastname: Gong
keywords: Anti-oxidant; Apoptosis; Luteolin; Osteosarcoma cells; Proliferation