title: Expression of biologically recombinant human acidic fibroblast growth factor in Arabidopsis thaliana seeds via oleosin fusion technology.
abstract: The potential of oleosins to act as carriers for recombinant foreign proteins in plant cells has been established. Using the oleosin fusion technology, the protein can be targeted to oil bodies in oilseeds by fusing it to the N- or C-terminus of oleosin. In this study, aFGF was expressed in Arabidopsis thaliana seeds via oleosin fusion technology. A plant-preferred aFGF gene was synthesized by optimizing codon usage and was fused to the C-terminus of the A. thaliana 18.5kDa oleosin gene. The fusion gene was driven by the phaseolin promoter to confer seed-specific expression of the human acidic fibroblast growth factor in A. thaliana. The T-DNA region of the recombinant plasmid pKO-aFGF was introduced into the genome of Arabidopsis thaliana by the floral dip method. The aFGF protein expression was confirmed by SDS-PAGE and western blotting. The biological activity showed that oil bodies fused to aFGF stimulated NIH/3T3 cell proliferation activity.
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Guan
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Linna
authors.initials: L
authors.lastname: Du
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Fawei
authors.initials: F
authors.lastname: Wang
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhen
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Qingman
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Deyi
authors.initials: D
authors.lastname: Zou
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Radiotherapy, The Second Hospital of Jilin University, Changchun 130021, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yu
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China; Jilin Agricultural University, Bioreactor Engineering Co., Ltd., Changchun 130118, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Li
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China; Jilin Agricultural University, Bioreactor Engineering Co., Ltd., Changchun 130118, China.
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D016220:Fibroblast Growth Factor 1 / Q000235:genetics*; D051379:Mice; D041681:NIH 3T3 Cells; D030821:Plants, Genetically Modified; D011994:Recombinant Proteins / Q000235:genetics*; D012639:Seeds / Q000235:genetics
chemical_list: D011994:Recombinant Proteins; D016220:Fibroblast Growth Factor 1
keywords: Acid fibroblast growth factor; Arabidopsis thaliana; Oil body; Oleosin