title: Biotransformation of luteoloside by a newly isolated human intestinal bacterium using UHPLC-Q-TOF/MS.
abstract: To explore the metabolic pathways and metabolites of luteoloside yielded by the isolated human intestinal bacteria from healthy human feces and characterize the β-d-glucosidase activity of the specific strain which catalyzed the breakdown of luteoloside, a preculture bacterial GAM broth and luteoloside were mixed incubated together for 48h. UHPLC-Q-TOF/MS was used for analysis of the metabolites of luteoloside in the corresponding supernatant fractions from fermentation. Aliquots of the reactive solutions were collected at different times and were measured with a microplate reader at 405nm to evaluate the enzymatic activity. Three metabolites (acetylated luteoloside, luteolin and deoxygenated luteolin) were detected in the fractions isolated from the bacterial samples. The variation of β-d-glucosidase activity inside the bacterium was in coincidence with the changes in luteolin generation or luteoloside degradation in different time periods.
authors.affiliation: Nantong University, Nantong 226001, PR China. Electronic address: taojinhua2000@163.com.
authors.forename: Jin-hua
authors.initials: JH
authors.lastname: Tao
authors.affiliation: Nantong University, Nantong 226001, PR China.
authors.forename: Dong-geng
authors.initials: DG
authors.lastname: Wang
authors.affiliation: Nantong University, Nantong 226001, PR China.
authors.forename: Chi
authors.initials: C
authors.lastname: Yang
authors.affiliation: Nantong University, Nantong 226001, PR China.
authors.forename: Jin-hua
authors.initials: JH
authors.lastname: Huang
authors.affiliation: Nantong University, Nantong 226001, PR China.
authors.forename: Wen-qian
authors.initials: WQ
authors.lastname: Qiu
authors.affiliation: Nantong University, Nantong 226001, PR China.
authors.forename: Xi
authors.initials: X
authors.lastname: Zhao
mesh_terms: D000328:Adult; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D016983:Enterococcus / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D005243:Feces / Q000382:microbiology*; D005260:Female; D005960:Glucosides / Q000378:metabolism*; D006801:Humans; D007422:Intestines / Q000382:microbiology; D013058:Mass Spectrometry; D053858:Metabolic Networks and Pathways; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D005960:Glucosides; D012336:RNA, Ribosomal, 16S; D001617:beta-Glucosidase
keywords: Enterococcus sp. 45; Enzyme activity; Luteoloside; Metabolic pathway; UPLC-Q-TOF/MS