title: Rio Grande do Norte, Brazil, voluntary bone marrow donors registry analysis.
abstract: OBJECTIVE
  this study aimed to report the allele and haplotype frequencies of volunteer bone marrow donors (VBMD) from the state of Rio Grande do Norte (RN) who were enrolled in the Brazilian Volunteer Bone Marrow Donor Registry (REDOME).
  
  
  METHODS
  the sample comprised 12,973 VBMD who had their allele and haplotype frequencies calculated by Arlequin 3.5.1.2. A multivariate analysis of the data was obtained through a principal component analysis (PCA) and hierarchical cluster analysis (HCA) performed with SPSS 8.0.
  
  
  RESULTS
  the most frequent allelic group was HLA-A*02, followed by -DRB1*13, -DRB1*04, -DRB1*07, -B*44, -B*35, -A*24 and -DRB1*01. Of the 2,701 haplotypes observed, the three most frequent were HLA-A*01 B*08 DRB1*03 (1.62%), -A*29 B*44 DRB1*07 (1.56%) and -A*02 B*44 DRB1*04 (1.29%). These haplotypes were in linkage disequilibrium. RN allele and haplotype frequencies were very similar to those in other Brazilian states in which similar studies have been performed. The PCA revealed that RN is highly genetically similar to Caucasian populations, especially those from Iberian countries, which strongly influenced the state's ethnic composition. Africans and Amerindians also influenced the RN population structure, to a lesser extent.
  
  
  CONCLUSIONS
  the HCA reinforced the conclusion that, despite its highly admixed profile, the RN population is genetically similar to European and European-descended populations. The PCA also showed that RN cities do not contribute to the same extent to REDOME, with less populous cities being underrepresented, indicating the need to enroll more VBMD from these smaller cities to faithfully depict the state's population structure in the database.
authors.affiliation: Federal University of Piauí, Teresina, Teresina, Brazil.
authors.forename: Marina Barguil
authors.initials: MB
authors.lastname: Macêdo
authors.affiliation: Federal University of Maringá, Maringá, PR, Brazil.
authors.forename: Luiza Tamie
authors.initials: LT
authors.lastname: Tsuneto
authors.affiliation: Dalton Cunha Blood Center, Natal, RN, Brazil.
authors.forename: Rosemary Almeida de Oliveira
authors.initials: RA
authors.lastname: Teixeira
authors.affiliation: Dalton Cunha Blood Center, Natal, RN, Brazil.
authors.forename: Maria do Socorro Belarmino de
authors.initials: Mdo S
authors.lastname: Oliveira
authors.affiliation: Department of Chemistry, Federal University of Piauí, Teresina, PI, Brazil.
authors.forename: José Machado
authors.initials: JM
authors.lastname: Moita Neto
authors.affiliation: Federal University of Piauí, Teresina, PI, Brazil.
authors.forename: Adalberto Socorro da
authors.initials: AS
authors.lastname: Silva
authors.affiliation: Department of Informatics and Statistics, Federal University of Piauí, Teresina, PI, Brazil.
authors.forename: Luiz Cláudio Demes da Mata
authors.initials: LC
authors.lastname: Sousa
authors.affiliation: Laboratory of Immunogenetics and Molecular Biology, Federal University of Piauí, Teresina, PI, Brazil.
authors.forename: Marayza Gomes
authors.initials: MG
authors.lastname: Carvalho
authors.affiliation: Federal University of Piauí, Teresina, Teresina, Brazil.
authors.forename: Herton Luiz Alves
authors.initials: HL
authors.lastname: Sales
authors.affiliation: Federal University of Piauí, Teresina, PI, Brazil.
authors.forename: José Renato Pereira de Moura
authors.initials: JR
authors.lastname: Barroso
authors.affiliation: Laboratory of Immunogenetics and Molecular Biology, Federal University of Piauí, Teresina, PI, Brazil.
authors.forename: Anaregina de Sousa
authors.initials: Ade S
authors.lastname: Araújo
authors.affiliation: Department of General Medicine, Federal University of Piauí, Teresina, PI, Brazil.
authors.forename: Semiramis Jamil Hadad do
authors.initials: SJ
authors.lastname: Monte
mesh_terms: D000328:Adult; D000483:Alleles; D001853:Bone Marrow; D001938:Brazil; D005260:Female; D005787:Gene Frequency / Q000235:genetics*; D015234:HLA-A Antigens / Q000235:genetics; D015235:HLA-B Antigens / Q000235:genetics; D059811:HLA-DRB1 Chains / Q000235:genetics; D006239:Haplotypes; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D015999:Multivariate Analysis; D012042:Registries; D014019:Tissue Donors
chemical_list: D015234:HLA-A Antigens; D015235:HLA-B Antigens; D059811:HLA-DRB1 Chains