title: Fast infrared spectroscopy of protein dynamics: advancing sensitivity and selectivity.
abstract: 2D-IR spectroscopy has matured to a powerful technique to study the structure and dynamics of peptides, but its extension to larger proteins is still in its infancy, the major limitations being sensitivity and selectivity. Site-selective information requires measuring single vibrational probes at sub-millimolar concentrations where most proteins are still stable, which is a severe challenge for conventional (FT)IR spectroscopy. Besides its ultrafast time-resolution, a so far largely underappreciated potential of 2D-IR spectroscopy lies in its sensitivity gain. The present paper sets the goals and outlines strategies how to use that sensitivity gain together with properly designed vibrational labels to make IR spectroscopy a versatile tool to study a wide class of proteins.
authors.affiliation: Department of Chemistry, University of Zurich, Winterthurerstr. 190, CH-8057 Zürich, Switzerland.
authors.forename: Klemens L
authors.initials: KL
authors.lastname: Koziol
authors.affiliation: Department of Chemistry, University of Zurich, Winterthurerstr. 190, CH-8057 Zürich, Switzerland.
authors.forename: Philip J M
authors.initials: PJ
authors.lastname: Johnson
authors.affiliation: Department of Chemistry, University of Zurich, Winterthurerstr. 190, CH-8057 Zürich, Switzerland.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Stucki-Buchli
authors.affiliation: Department of Chemistry, University of Zurich, Winterthurerstr. 190, CH-8057 Zürich, Switzerland.
authors.forename: Steven A
authors.initials: SA
authors.lastname: Waldauer
authors.affiliation: Department of Chemistry, University of Zurich, Winterthurerstr. 190, CH-8057 Zürich, Switzerland. Electronic address: peter.hamm@chem.uzh.ch.
authors.forename: Peter
authors.initials: P
authors.lastname: Hamm
mesh_terms: D008958:Models, Molecular; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013055:Spectrophotometry, Infrared / Q000379:methods / Q000592:standards
chemical_list: D011506:Proteins