title: Plasma retinoids concentration in Leptodactylus chaquensis (Amphibia: Leptodactylidae) from rice agroecosystems, Santa Fe province, Argentina.
abstract: Retinoids are known to regulate important processes such as differentiation, development, and embryogenesis of vertebrates: Alteration in endogenous retinoids concentration is linked with teratogenic effects. Retinol (ROH), retinoid acid (RA), and isoform 13-Cis-retinoic acid (13-Cis-RA), in plasma of a native adults frog, Leptodactylus chaquensis from a rice field (RF) and a forest (reference site; RS) were measured. ROH did not vary between treatment sites. RA and 13-Cis-RA activities were higher (93.7±8.6 μg mL(-1) and 131.7±11.4 μg mL(-1), respectively) in individuals collected from RF than in those from RS (65.5±8.6 μg mL(-1) and 92.2±10.2 μg mL(-1), respectively). The ratios retinoic acid-retinol (RA/ROH) and 13-Cis-RA/ROH revealed significantly higher values in RF than in RS. RA and 13-Cis-RA concentrations in plasma on wild amphibian's species such as L. chaquensis would be suitable biomarkers of pesticide exposure in field monitoring. Finally, the mechanism of alteration in retinoid metabolites alteration should be further explored both in larvae and adult, considering that the potential exposition and uptake contaminants vary between the double lives of these vertebrates.
authors.affiliation: Laboratorio de Desarrollo Analítico y Quimiometría (LADAQ), Cátedra de Química Analítica I, Facultad de Bioquímica y Ciencias Biológicas (FBCB), Universidad Nacional del Litoral (UNL), C.C. 242, S3000ZAA Santa Fe, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
authors.forename: Carla M
authors.initials: CM
authors.lastname: Teglia
authors.affiliation: Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina; Laboratorio de Ecotoxicología (FBCB-UNL), C.C. 242, S3000ZAA Santa Fe, Argentina. Electronic address: mattademo@hotmail.com.
authors.forename: Andrés M
authors.initials: AM
authors.lastname: Attademo
authors.affiliation: Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina; Laboratorio de Ecotoxicología (FBCB-UNL), C.C. 242, S3000ZAA Santa Fe, Argentina.
authors.forename: Paola M
authors.initials: PM
authors.lastname: Peltzer
authors.affiliation: Laboratorio de Desarrollo Analítico y Quimiometría (LADAQ), Cátedra de Química Analítica I, Facultad de Bioquímica y Ciencias Biológicas (FBCB), Universidad Nacional del Litoral (UNL), C.C. 242, S3000ZAA Santa Fe, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
authors.forename: Héctor C
authors.initials: HC
authors.lastname: Goicoechea
authors.affiliation: Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina; Laboratorio de Ecotoxicología (FBCB-UNL), C.C. 242, S3000ZAA Santa Fe, Argentina.
authors.forename: Rafael C
authors.initials: RC
authors.lastname: Lajmanovich
mesh_terms: D000328:Adult; D000383:Agriculture; D000818:Animals; D001001:Anura / Q000097:blood*; D001118:Argentina; D017753:Ecosystem; D004784:Environmental Monitoring; D006801:Humans; D007814:Larva; D012275:Oryza; D011898:Ranidae / Q000097:blood*; D012176:Retinoids / Q000097:blood*; D014212:Tretinoin
chemical_list: D012176:Retinoids; D014212:Tretinoin
keywords: Amphibians; L. chaquensis; Pesticides; Retinoic acid