title: Evaluation of Sustained BMP-2 Release Profiles Using a Novel Fluorescence-Based Retention Assay.
abstract: The purpose of this study was to develop and characterize a novel fluorescence-based retention assay for the evaluation of the release profile of bone morphogenetic protein-2 (BMP-2) released from bone graft carrier. In this study, we evaluated the binding, release kinetics, and delivery efficacies of BMP-2 incorporated into hydroxyapatite (HA) bone grafts. The evaluation of the release profile of BMP-2 from HA bone grafts using a fluorescence-based retention assay revealed initial burst releases from the HA bone grafts followed by long sustained releases up to 14 weeks. The sustained biological activity of the released BMP-2 from HA bone grafts over the full 14-week period supports a long sustained mechanism via fluorescence-based retention assay. Thus, the results from this study show that BMP-2 could be incorporated into HA bone grafts for sustained release over a prolonged period of time with retention of bioactivity and our fluorescence-based retention assay, which is principally detecting the retention profile of BMP-2 in HA bone grafts, is more accurate than conventionally collecting the released BMP-2 for evaluation of BMP-2 release profiles.
authors.affiliation: Department of Biochemistry, Inha University School of Medicine, Incheon, Republic of Korea.
authors.forename: Wonmo
authors.initials: W
authors.lastname: Kang
authors.affiliation: Department of Biomedical Chemistry, Konkuk University, Chung-Ju, Republic of Korea.
authors.forename: Dong-Sung
authors.initials: DS
authors.lastname: Lee
authors.affiliation: Department of Biochemistry, Inha University School of Medicine, Incheon, Republic of Korea.
authors.forename: Jun-Hyeog
authors.initials: JH
authors.lastname: Jang
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics / Q000378:metabolism*; D016025:Bone Transplantation; D002460:Cell Line; D017886:Durapatite / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods*; D015202:Protein Engineering; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
chemical_list: D055396:Bone Morphogenetic Protein 2; D011993:Recombinant Fusion Proteins; D017886:Durapatite