title: Structures and gene clusters of the closely related O-antigens of Escherichia coli O46 and O134, both containing D-glucuronoyl-D-allothreonine.
abstract: The O-polysaccharides (O-antigens) were isolated by mild acid degradation of the lipopolysaccharide (LPS) of Escherichia coli O46 and O134. The structures of their linear tetrasaccharide repeating units were established by sugar analysis along with 1D and 2D (1)H and (13)C NMR spectroscopy: [Formula: see text], where D-aThr indicates D-allothreonine and R indicates O-acetyl substitution (∼ 70% on aThr and ∼ 15% on GalNAc) in E. coli O46 whereas the O-acetylation is absent in E. coli O134. Functions of genes in the essentially identical O-antigen gene clusters of E. coli O46 and O134 were tentatively assigned by a comparison with sequences in available databases and found to be in agreement with the O-polysaccharide structures established.
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation. Electronic address: perepel@ioc.ac.ru.
authors.forename: Andrei V
authors.initials: AV
authors.lastname: Perepelov
authors.affiliation: TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, 300457 Tianjin, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Wang
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors.forename: Andrei V
authors.initials: AV
authors.lastname: Filatov
authors.affiliation: TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, 300457 Tianjin, China.
authors.forename: Xianghong
authors.initials: X
authors.lastname: Xia
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.affiliation: Department of Laboratory Medicine, Division of Clinical Microbiology, Karolinska Institute, Karolinska University Hospital, S-141 86 Stockholm, Sweden.
authors.forename: Andrej
authors.initials: A
authors.lastname: Weintraub
authors.affiliation: Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, S-106 91 Stockholm, Sweden.
authors.forename: Göran
authors.initials: G
authors.lastname: Widmalm
authors.affiliation: TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, 300457 Tianjin, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
mesh_terms: D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D019081:O Antigens / Q000737:chemistry*
chemical_list: D019081:O Antigens
keywords: Allothreonine; Bacterial polysaccharide structure; Escherichia coli; O-antigen; O-antigen gene cluster; O-polysaccharide