title: Effects of high glucose on expression of OPG and RANKL in rat aortic vascular smooth muscle cells.
abstract: OBJECTIVE
  To explore effect of high glucose on expression of osteoprotegerin (OPG) and receptor activator of NF- κ B ligand (RANKE) in rat aortic vascular smooth muscle cells.
  
  
  METHODS
  SD rats were intraperitoneally injected with streptozotocin, OPG and RANKL expression in rat thoracic aortas were detected by immunohistochemical staining. In cultured vascular smooth muscle cells (VSMCs) (A7r5), qRT-PCR and Western blot analysis were used to examine the mRNA and protein levels of OPG and RANKL.
  
  
  RESULTS
  Our results demonstrated that OPG expression was increased in hyperglycemic rat aortic VSMCs, while RANKL expression was decreased. Besides, in vitro experiments high glucose induced OPG expression, but depressed RANKL expression by dose- and time-dependent manner in cultured A7r5.
  
  
  CONCLUSIONS
  Our findings suggested that high glucose could promote the expression of OPG, and inhibit the expression of RANKL in VSMCs, which may be partly be the molecular mechanism of diabetic vascular calcification.
authors.affiliation: Department of Neurology, the Second People's Hospital of Xinxiang, Xinxiang 453002, PR China.
authors.forename: Hong-Juan
authors.initials: HJ
authors.lastname: Chang
authors.affiliation: Department of Cardiovascular Institute, Affiliated Hospital of Hainan Medical College, Haikou 571199, PR China.
authors.forename: Tian-Fa
authors.initials: TF
authors.lastname: Li
authors.affiliation: Department of Cardiovascular Institute, Affiliated Hospital of Hainan Medical College, Haikou 571199, PR China.
authors.forename: Jun-Li
authors.initials: JL
authors.lastname: Guo
authors.affiliation: Department of Cardiovascular Institute, Affiliated Hospital of Hainan Medical College, Haikou 571199, PR China.
authors.forename: You-Ling
authors.initials: YL
authors.lastname: Lan
authors.affiliation: Department of Cardiovascular Institute, Affiliated Hospital of Hainan Medical College, Haikou 571199, PR China.
authors.forename: Yue-Qiong
authors.initials: YQ
authors.lastname: Kong
authors.affiliation: Department of Neurology, the Second People's Hospital of Xinxiang, Xinxiang 453002, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Meng
authors.affiliation: Department of Cardiovascular Institute, Affiliated Hospital of Hainan Medical College, Haikou 571199, PR China.
authors.forename: Xian-Ji
authors.initials: XJ
authors.lastname: Ma
authors.affiliation: National Center for International Research of Biological Targeting Diagnosis and Therapy, Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Guangxi Medical University, Nanning 530021, PR China.
authors.forename: Xiao-Ling
authors.initials: XL
authors.lastname: Lu
authors.affiliation: Reproductive Medicine Center, Affiliated Hospital of Hainan Medical College, Haikou 570102, PR China.
authors.forename: Wei-Ying
authors.initials: WY
authors.lastname: Lu
authors.affiliation: Department of Pathology, Affiliated Hospital of Hainan Medical College, Haikou 570102, PR China.
authors.forename: Shao-Jiang
authors.initials: SJ
authors.lastname: Zheng
keywords: High glucose; Osteoprotegerin; Receptor activator of NF– κ B ligand; Vascular smooth muscle cells