title: Molecular cloning and functional characterization of cyclophilin A in yellow catfish (Pelteobagrus fulvidraco).
abstract: Cyclophilin A (CypA) is a ubiquitously expressed protein which involves in diverse pathological conditions including infection and inflammation. In this report, a CypA gene (designated as YC-CypA) was cloned from yellow catfish (Pelteobagrus fulvidraco) which is an important cultured fish species in Asian countries. The open reading frame (ORF) of YC-CypA encoded a polypeptide of 164 amino acids with calculated molecular weight of 17.70 kDa. The deduced amino acid sequences of the YC-CypA shared highly conserved structures with CypAs from the other species, indicating that YC-CypA should be a new member of the CypA family. Full-length YC-CypA protein was expressed in Escherichia coli and specific polyclonal antibody against YC-CypA was generated. The YC-CypA protein showed chemotactic activity by transwell migration assay. The mRNA and protein of YC-CypA could be detected in all examined tissues with relatively higher mRNA level in spleen and higher protein level in head kidney, respectively. The temporal expression patterns of YC-CypA, IL-1β and TNF-α mRNAs were analyzed in the liver, spleen and head kidney post of Edwardsiella ictaluri infection. By immunohistochemistry assay, slight enhancement of YC-CypA protein was observed in the liver, spleen, body kidney and head kidney of yellow catfish infected with E. ictaluri. In conclusion, YC-CypA of yellow catfish showed chemotactic activity in vitro and might have been involved in cytokines secretion in yellow catfish during the infection of E. ictaluri.
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, Hubei 430070, China.
authors.forename: Xingxing
authors.initials: X
authors.lastname: Dong
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, Hubei 430070, China.
authors.forename: Zhendong
authors.initials: Z
authors.lastname: Qin
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, China; School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan, Hubei 430023, China.
authors.forename: Xianqin
authors.initials: X
authors.lastname: Hu
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, Hubei 430070, China.
authors.forename: Jiangfeng
authors.initials: J
authors.lastname: Lan
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, Hubei 430070, China.
authors.forename: Gailing
authors.initials: G
authors.lastname: Yuan
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Asim
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, Hubei 430070, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Wuhan Fishery Research Institute, Wuhan, Hubei 430207, China.
authors.forename: Taoshan
authors.initials: T
authors.lastname: Ai
authors.affiliation: Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, Hubei 430070, China; Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, Hubei 430070, China. Electronic address: Jmei@mail.hzau.edu.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Mei
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, Hubei 430070, China; Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, Hubei 430070, China. Electronic address: linli@mail.hzau.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Lin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002397:Catfishes / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D002465:Cell Movement; D003001:Cloning, Molecular; D021984:Cyclophilin A / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D020610:Edwardsiella ictaluri; D004756:Enterobacteriaceae Infections / Q000276:immunology / Q000378:metabolism; D005393:Fish Diseases / Q000276:immunology / Q000378:metabolism; D029941:Fish Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D060226:Head Kidney / Q000378:metabolism; D053583:Interleukin-1beta / Q000235:genetics; D007668:Kidney / Q000378:metabolism; D007962:Leukocytes / Q000276:immunology; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D013154:Spleen / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: D029941:Fish Proteins; D053583:Interleukin-1beta; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D021984:Cyclophilin A
keywords: Chemotactic activity; Cyclophilin A; Edwardsiella ictaluri; Expression; Pelteobagrus fulvidraco