title: Molecular Remodeling of the Presynaptic Active Zone of Drosophila Photoreceptors via Activity-Dependent Feedback.
abstract: Neural activity contributes to the regulation of the properties of synapses in sensory systems, allowing for adjustment to a changing environment. Little is known about how synaptic molecular components are regulated to achieve activity-dependent plasticity at central synapses. Here, we found that after prolonged exposure to natural ambient light the presynaptic active zone in Drosophila photoreceptors undergoes reversible remodeling, including loss of Bruchpilot, DLiprin-α, and DRBP, but not of DSyd-1 or Cacophony. The level of depolarization of the postsynaptic neurons is critical for the light-induced changes in active zone composition in the photoreceptors, indicating the existence of a feedback signal. In search of this signal, we have identified a crucial role of microtubule meshwork organization downstream of the divergent canonical Wnt pathway, potentially via Kinesin-3 Imac. These data reveal that active zone composition can be regulated in vivo and identify the underlying molecular machinery.
authors.affiliation: Dendrite Differentiation, German Center for Neurodegenerative Diseases (DZNE), Bonn 53175, Germany.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Sugie
authors.affiliation: Core Division of Advanced Research, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology (Titech), Yokohama 226-8501, Japan.
authors.forename: Satoko
authors.initials: S
authors.lastname: Hakeda-Suzuki
authors.affiliation: Gene Network Laboratory, National Institute of Genetics and Department of Genetics, SOKENDAI, Mishima 411-8540, Japan.
authors.forename: Emiko
authors.initials: E
authors.lastname: Suzuki
authors.affiliation: European Neuroscience Institute (ENI), 37077 Göttingen, Germany.
authors.forename: Marion
authors.initials: M
authors.lastname: Silies
authors.affiliation: Core Division of Advanced Research, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology (Titech), Yokohama 226-8501, Japan.
authors.forename: Mai
authors.initials: M
authors.lastname: Shimozono
authors.affiliation: Image and Data Analysis Facility, German Center for Neurodegenerative Diseases (DZNE), Bonn 53175, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Möhl
authors.affiliation: Core Division of Advanced Research, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology (Titech), Yokohama 226-8501, Japan. Electronic address: suzukit@bio.titech.ac.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Suzuki
authors.affiliation: Dendrite Differentiation, German Center for Neurodegenerative Diseases (DZNE), Bonn 53175, Germany. Electronic address: gaia.tavosanis@dzne.de.
authors.forename: Gaia
authors.initials: G
authors.lastname: Tavosanis
mesh_terms: D000818:Animals; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D025461:Feedback, Physiological / Q000502:physiology*; D047908:Intracellular Signaling Peptides and Proteins; D007473:Ion Channels; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008822:Mice, Transgenic; D046529:Microscopy, Electron, Transmission; D008954:Models, Biological; D010641:Phenotype; D010750:Phosphoproteins / Q000378:metabolism; D010775:Photic Stimulation; D017956:Photoreceptor Cells, Invertebrate / Q000145:classification / Q000166:cytology* / Q000378:metabolism; D017729:Presynaptic Terminals / Q000502:physiology* / Q000648:ultrastructure; D015398:Signal Transduction / Q000235:genetics; D013569:Synapses / Q000502:physiology / Q000648:ultrastructure; D000074025:TRPA1 Cation Channel; D050052:TRPC Cation Channels / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: C510146:BRP protein, Drosophila; D029721:Drosophila Proteins; C557617:GAL4 protein, Drosophila; D047908:Intracellular Signaling Peptides and Proteins; D007473:Ion Channels; C490600:Liprin-alpha protein, Drosophila; D008164:Luminescent Proteins; D010750:Phosphoproteins; D000074025:TRPA1 Cation Channel; D050052:TRPC Cation Channels; D014157:Transcription Factors; C475684:TrpA1 protein, Drosophila