title: Comparison of human mesenchymal stem cells proliferation and differentiation on poly(methyl methacrylate) bone cements with and without mineralized collagen incorporation.
abstract: Poly(methyl methacrylate) bone cement is widely used in vertebroplasty, joint replacement surgery, and other orthopaedic surgeries, while it also exposed many problems on mechanical property and biocompatibility. Better performance in mechanical match and bone integration is highly desirable. Recently, there reported that incorporation of mineralized collagen into poly(methyl methacrylate) showed positive results in mechanical property and osteointegration ability in vivo. In the present study, we focused on the comparison of osteogenic behavior between mineralized collagen incorporated in poly(methyl methacrylate) and poly(methyl methacrylate). Human marrow mesenchymal stem cells are used in this experiment. Adhesion and proliferation were used to characterize biocompatibility. Activity of alkaline phosphatase was used to assess the differentiation of human marrow mesenchymal stem cells into osteoblasts. Real-time PCR was performed to detect the expression of osteoblast-related markers at messenger RNA level. The results show that osteogenic differentiation on mineralized collagen incorporated in poly(methyl methacrylate) bone cement is more than two times higher than that of poly(methyl methacrylate) after culturing for 21 days. Thus, important mechanism on mineralized collagen incorporation increasing the osteogenetic ability of poly(methyl methacrylate) bone cement may be understood in this concern.
authors.affiliation: School of Engineering and Technology, China University of Geosciences (Beijing), Beijing, China Institute of Regenerative Medical Materials, School of Materials Science and Engineering, Tsinghua University, Beijing, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Wu
authors.affiliation: Institute of Regenerative Medical Materials, School of Materials Science and Engineering, Tsinghua University, Beijing, China.
authors.forename: Suju
authors.initials: S
authors.lastname: Xu
authors.affiliation: Institute of Regenerative Medical Materials, School of Materials Science and Engineering, Tsinghua University, Beijing, China.
authors.forename: Zhiye
authors.initials: Z
authors.lastname: Qiu
authors.affiliation: School of Stomatology, Dalian Medical University, Dalian, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.affiliation: School of Stomatology, Dalian Medical University, Dalian, China.
authors.forename: Huiying
authors.initials: H
authors.lastname: Liu
authors.affiliation: School of Materials Science and Technology, China University of Geosciences (Beijing), Beijing, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Yu
authors.affiliation: Institute of Regenerative Medical Materials, School of Materials Science and Engineering, Tsinghua University, Beijing, China cuifz@mail.tsinghua.edu.cn yuxiang690625@aliyun.com.
authors.forename: Fu-Zhai
authors.initials: FZ
authors.lastname: Cui
authors.affiliation: Chinese Academy of Medical Sciences, Institute of Basic Medical Science, Beijing, China.
authors.forename: Zhao Robert
authors.initials: ZR
authors.lastname: Chunhua
mesh_terms: D001843:Bone Cements / Q000737:chemistry*; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology*; D008903:Minerals / Q000737:chemistry*; D010012:Osteogenesis / Q000502:physiology; D019904:Polymethyl Methacrylate / Q000138:chemical synthesis*
chemical_list: D001843:Bone Cements; D008903:Minerals; D003094:Collagen; D019904:Polymethyl Methacrylate
keywords: Bone cement; PMMA; human mesenchymal stem cells; mineralized collagen; osteogenic differentiation