title: Simultaneous determination of ascaridole, p-cymene and α-terpinene in rat plasma after oral administration of Chenopodium ambrosioides L. by GC-MS.
abstract: A sensitive and reliable GC-MS method was developed and validated for the simultaneous determination of ascaridole, p-cymene and α-terpinene in rat plasma using naphthalene as internal standard. The plasma samples were extracted with ethyl acetate. Chromatographic separation was carried out on a HP-5MS capillary analytical column (30 m × 0.25 mm, 0.25 µm) and detection was performed on a quadrupole mass spectrometer detector operated under selected ion monitoring mode. The method showed excellent linearity over the investigated concentration range (r > 0.99) with the limit of quantitation down to 50, 10 and 5 ng/mL for ascaridole, p-cymene and α-terpinene, respectively. The intra-day and inter-day precisions (RSD) were <11.3%, and the accuracy was between 90.7 and 113.8%. The method was successfully applied to investigate the pharmacokinetics of Chenopodium ambrosioides L. following oral administration to rats.
authors.affiliation: Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environmental Science, Hebei University, Baoding, 071002, China.|Tasly Academy, Tasly Holding Group Co. Ltd, Tianjin, 300410, China.
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Hu
authors.affiliation: Tasly Academy, Tasly Holding Group Co. Ltd, Tianjin, 300410, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Chu
authors.affiliation: Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environmental Science, Hebei University, Baoding, 071002, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Ma
authors.affiliation: Tasly Academy, Tasly Holding Group Co. Ltd, Tianjin, 300410, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Tasly Academy, Tasly Holding Group Co. Ltd, Tianjin, 300410, China.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Tasly Academy, Tasly Holding Group Co. Ltd, Tianjin, 300410, China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Mo
authors.affiliation: Tasly Academy, Tasly Holding Group Co. Ltd, Tianjin, 300410, China.
authors.forename: Qihui
authors.initials: Q
authors.lastname: Yin
authors.affiliation: Tasly Academy, Tasly Holding Group Co. Ltd, Tianjin, 300410, China.
authors.forename: Jiahua
authors.initials: J
authors.lastname: Guo
authors.affiliation: Tasly Academy, Tasly Holding Group Co. Ltd, Tianjin, 300410, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Ma
authors.affiliation: Tasly Academy, Tasly Holding Group Co. Ltd, Tianjin, 300410, China.
authors.forename: Shuiping
authors.initials: S
authors.lastname: Zhou
mesh_terms: D000284:Administration, Oral; D000818:Animals; D019540:Area Under Curve; D027464:Chenopodium ambrosioides / Q000737:chemistry*; D000081005:Cyclohexane Monoterpenes; D000081007:Cymenes; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006207:Half-Life; D057230:Limit of Detection; D008297:Male; D039821:Monoterpenes / Q000097:blood* / Q000493:pharmacokinetics; D010545:Peroxides / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D012015:Reference Standards; D015203:Reproducibility of Results
chemical_list: D000081005:Cyclohexane Monoterpenes; D000081007:Cymenes; D039821:Monoterpenes; D010545:Peroxides; C043372:ascaridole; C007210:4-cymene; C018669:gamma-terpinene
keywords: GC-MS; ascaridole; p-cymene; pharmacokinetic study; α-terpinene