title: Depletion of neutrophils protects against L-arginine-induced acute pancreatitis in mice.
abstract: OBJECTIVE
  Acute pancreatitis (AP) is an inflammatory disease characterized by acinar cell damage and inflammation of the pancreas with infiltration of leukocytes, predominantly neutrophils. We investigated whether neutrophil depletion protects against experimental AP induced by L-arginine.
  
  
  METHODS
  AP was induced in C57BL/6 mice via two intraperitoneal L-arginine (4 g/kg) injections. Mice were pretreated with 250 and 100 µg anti-Gr-1 antibody via intraperitoneal injection at 24 and 4 h, respectively, before L-arginine challenge for neutrophil depletion. At 48 and 72 h after injection, the severity of AP was determined with the aid of biochemical and histological analyses. Amylase and MPO activity was detected using specific assay kits. The plasma cytokines levels were detected using ELISA.
  
  
  RESULTS
  Neutrophil depletion resulted in significantly reduced plasma amylase levels in pancreas, myeloperoxidase (MPO) activity in pancreas and lung, reactive oxygen species (ROS) generation and cell apoptosis, and decreased circulating neutrophil, tissue damage as well as expression levels of nuclear factor NF-κB.
  
  
  CONCLUSIONS
  Neutrophil depletion is capable of reducing tissue damage of pancreas and lung in mice with acute pancreatitis.
authors.affiliation: Emergency Department, The First Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: Guolin
authors.initials: G
authors.lastname: Chen
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Shiqi
authors.initials: S
authors.lastname: Lu
mesh_terms: D000208:Acute Disease; D000681:Amylases / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001120:Arginine / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D008168:Lung / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism* / Q000502:physiology*; D010179:Pancreas / Q000378:metabolism / Q000503:physiopathology; D010195:Pancreatitis / Q000139:chemically induced* / Q000378:metabolism / Q000503:physiopathology*; D009195:Peroxidase / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D016207:Cytokines; D016328:NF-kappa B; D017382:Reactive Oxygen Species; D001120:Arginine; D009195:Peroxidase; D000681:Amylases