title: Control of biomimetic hydroxyapatite deposition on polymer substrates using different protein adsorption abilities.
abstract: We recently developed a system for coating polystyrene (PS) substrates with hydroxyapatite (HAp) by utilizing serum protein adsorption layers as mediators to induce the heterogeneous nucleation of HAp in simulated body fluids (SBFs). In this study, the selective deposition of HAp on polymer substrate surfaces with different protein adsorption abilities was investigated using PS and poly(methyl methacrylate) (PMMA). Atomic force microscopic observations and the results of a quantitative analysis using a quartz-crystal microbalance (QCM) revealed that the amounts of proteins such as human serum albumin (HSA) and human immunoglobulin G (hIgG) adsorbed on PS substrate surfaces were markedly greater than those on PMMA substrate surfaces. A markedly larger amount of HAp was deposited on protein-treated PS substrate surfaces than on PMMA substrate surfaces, reflecting protein adsorption to polymers. We also revealed that the deposition of HAp on protein-adsorbed PS substrate surfaces was enhanced by aqueous calcium chloride treatments before immersion in 1.5SBF. In the case of 2.5 M calcium chloride treatment, these surfaces were completely covered with deposits.
authors.affiliation: Department of Industrial Chemistry, Faculty of Engineering, Tokyo University of Science, 12-1 Ichigayafunagawara-machi, Shinjuku-ku, Tokyo 162-0826, Japan.
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Iijima
authors.affiliation: Department of Industrial Chemistry, Faculty of Engineering, Tokyo University of Science, 12-1 Ichigayafunagawara-machi, Shinjuku-ku, Tokyo 162-0826, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Sakai
authors.affiliation: Department of Industrial Chemistry, Faculty of Engineering, Tokyo University of Science, 12-1 Ichigayafunagawara-machi, Shinjuku-ku, Tokyo 162-0826, Japan.
authors.forename: Akinori
authors.initials: A
authors.lastname: Komori
authors.affiliation: Department of Industrial Chemistry, Faculty of Engineering, Tokyo University of Science, 12-1 Ichigayafunagawara-machi, Shinjuku-ku, Tokyo 162-0826, Japan.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Sakamoto
authors.affiliation: Department of Applied Chemistry, Kyushu University, 744 Nishi-ku, Motooka, Fukuoka 819-0395, Japan.
authors.forename: Hisao
authors.initials: H
authors.lastname: Matsuno
authors.affiliation: Department of Organic and Polymeric Materials, Graduate School of Science and Engineering, Tokyo Institute of Technology, 2-12-1-H121 Ookayama, Meguro-ku, Tokyo 152-8550, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Serizawa
authors.affiliation: Department of Industrial Chemistry, Faculty of Engineering, Tokyo University of Science, 12-1 Ichigayafunagawara-machi, Shinjuku-ku, Tokyo 162-0826, Japan. Electronic address: mhashizu@ci.kagu.tus.ac.jp.
authors.forename: Mineo
authors.initials: M
authors.lastname: Hashizume
mesh_terms: D000327:Adsorption / Q000187:drug effects; D040761:Biomimetic Materials / Q000737:chemistry; D002122:Calcium Chloride / Q000494:pharmacology; D017886:Durapatite / Q000737:chemistry*; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D011108:Polymers / Q000737:chemistry*; D019904:Polymethyl Methacrylate / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D058525:Quartz Crystal Microbalance Techniques; D012709:Serum Albumin / Q000737:chemistry; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
chemical_list: D007074:Immunoglobulin G; D011108:Polymers; D011137:Polystyrenes; D011506:Proteins; D012709:Serum Albumin; D019904:Polymethyl Methacrylate; D017886:Durapatite; D002122:Calcium Chloride
keywords: Hydroxyapatite; Poly(methyl methacrylate); Polystyrene; Serum protein; Simulated body fluid