title: Cardiac protein changes in rats after soybean oil treatment: a proteomic study.
abstract: Several studies show that the consumption of vegetable oils, such as soybean oil, rich in polyunsaturated fatty acids (PUFAs) has beneficial health effects by preventing or reducing the risk factors of cardiovascular diseases. While the demonstration of beneficial effects of the consumption of unsaturated fatty acids on the cardiovascular system has been proven in a macroscopic level, the molecular/cellular mechanisms responsible for this phenomenon are poorly understood.
  
  
  
  In this work, a comparative proteomic approach, two-dimensional gel electrophoresis (2-DE) coupled to mass spectrometry (MALDI-TOF/TOF), was applied to investigate proteome differences in the left ventricle (LV) of rats that received 0.1 mL of soybean oil intramuscularly for 15 days (treated group-TR) and rats that had not (control group-CT).
  
  
  
  Soybean oil treatment improved left ventricular function, TR animals presented lower value of LVEDP and significantly changed LV proteome. The protein profile of VE revealed differences in the expression of 60 protein spots (p<0.05) between the experimental groups (CT and TR), 14 of those were identified by MS and MS/MS, and 12 of the 14 being non-redundant proteins. Robust changes were detected in proteins involved in cellular structure and antioxidant system and muscular contraction.
  
  
  
  The TR group presented an increase in the intensity of proteins involved in muscle contraction (myosin light chain-3 (3-MCL), creatine kinase M (CKM)) and thireodoxin, an antioxidant enzyme. Low intensity cytoskeletal protein, desmin, was also detected in TR animals. The results suggest that soybean oil induces changes in the levels of heart proteins which may partially account for the underlying mechanisms involved in the benefits provided by oils rich in polyunsaturated fatty acids.
authors.affiliation: Pós-Graduação em Ciências Fisiológicas, Federal University of Espírito Santo (UFES), Av. Marechal Campos,1468, Maruípe, Vitória, Espírito Santo, Brazil. tatasoprani@yahoo.com.br.
authors.forename: Taisla
authors.initials: T
authors.lastname: Soprani
authors.affiliation: Pós-Graduação em Ciências Fisiológicas, Federal University of Espírito Santo (UFES), Av. Marechal Campos,1468, Maruípe, Vitória, Espírito Santo, Brazil. viniciuskiffer@hotmail.com.
authors.forename: Vinicius Kuffer
authors.initials: VK
authors.lastname: Uliana
authors.affiliation: Pós-Graduação em Ciências Fisiológicas, Federal University of Espírito Santo (UFES), Av. Marechal Campos,1468, Maruípe, Vitória, Espírito Santo, Brazil. faustino43@oi.com.br.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Hertel Ribeiro
authors.affiliation: Pós-Graduação em Ciências Fisiológicas, Federal University of Espírito Santo (UFES), Av. Marechal Campos,1468, Maruípe, Vitória, Espírito Santo, Brazil. sergiolisboajr@gmail.com.
authors.forename: Sergio
authors.initials: S
authors.lastname: Lisboa
authors.affiliation: Pós-Graduação em Ciências Fisiológicas, Federal University of Espírito Santo (UFES), Av. Marechal Campos,1468, Maruípe, Vitória, Espírito Santo, Brazil. gabriella_xm@yahoo.com.br.
authors.forename: Gabriella Xavier
authors.initials: GX
authors.lastname: Maretto
authors.affiliation: Laboratório de Toxinologia, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brazil. atsferreira@ioc.fiocruz.br.
authors.forename: André Teixeira Silva
authors.initials: AT
authors.lastname: da Ferreira
authors.affiliation: Laboratório de Toxinologia, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brazil. jonasperales@gmail.com.
authors.forename: Jonas
authors.initials: J
authors.lastname: Perales
authors.affiliation: Pós-Graduação em Ciências Fisiológicas, Federal University of Espírito Santo (UFES), Av. Marechal Campos,1468, Maruípe, Vitória, Espírito Santo, Brazil. ivanitaufes@hotmail.com.
authors.forename: Ivanita
authors.initials: I
authors.lastname: Stefanon
authors.affiliation: Pós-Graduação em Ciências Fisiológicas, Federal University of Espírito Santo (UFES), Av. Marechal Campos,1468, Maruípe, Vitória, Espírito Santo, Brazil. suelygf@gmail.com.
authors.forename: Suely Gomes
authors.initials: SG
authors.lastname: de Figueiredo
mesh_terms: D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D006352:Heart Ventricles / Q000737:chemistry / Q000187:drug effects*; D007273:Injections, Intramuscular; D008297:Male; D011506:Proteins / Q000032:analysis; D040901:Proteomics / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D013024:Soybean Oil / Q000008:administration & dosage / Q000494:pharmacology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D016277:Ventricular Function, Left / Q000187:drug effects
chemical_list: D011506:Proteins; D013024:Soybean Oil