title: The coordinated action of the MVB pathway and autophagy ensures cell survival during starvation.
abstract: The degradation and recycling of cellular components is essential for cell growth and survival. Here we show how selective and non-selective lysosomal protein degradation pathways cooperate to ensure cell survival upon nutrient limitation. A quantitative analysis of starvation-induced proteome remodeling in yeast reveals comprehensive changes already in the first three hours. In this period, many different integral plasma membrane proteins undergo endocytosis and degradation in vacuoles via the multivesicular body (MVB) pathway. Their degradation becomes essential to maintain critical amino acids levels that uphold protein synthesis early during starvation. This promotes cellular adaptation, including the de novo synthesis of vacuolar hydrolases to boost the vacuolar catabolic activity. This order of events primes vacuoles for the efficient degradation of bulk cytoplasm via autophagy. Hence, a catabolic cascade including the coordinated action of the MVB pathway and autophagy is essential to enter quiescence to survive extended periods of nutrient limitation.
authors.affiliation: Division of Cell Biology, Biocenter, Medical University of Innsbruck, Innsbruck, Austria.
authors.forename: Martin
authors.initials: M
authors.lastname: Müller
authors.affiliation: Division of Cell Biology, Biocenter, Medical University of Innsbruck, Innsbruck, Austria.
authors.forename: Oliver
authors.initials: O
authors.lastname: Schmidt
authors.affiliation: Division of Bioinformatics, Biocenter, Medical University of Innsbruck, Innsbruck, Austria.
authors.forename: Mihaela
authors.initials: M
authors.lastname: Angelova
authors.affiliation: Division of Clinical Biochemistry, ProteinMicroAnalysis Facility, Biocenter, Medical University of Innsbruck, Innsbruck, Austria.
authors.forename: Klaus
authors.initials: K
authors.lastname: Faserl
authors.affiliation: Division of Cell Biology, Biocenter, Medical University of Innsbruck, Innsbruck, Austria.
authors.forename: Sabine
authors.initials: S
authors.lastname: Weys
authors.affiliation: Division of Clinical Biochemistry, ProteinMicroAnalysis Facility, Biocenter, Medical University of Innsbruck, Innsbruck, Austria.
authors.forename: Leopold
authors.initials: L
authors.lastname: Kremser
authors.affiliation: Max F. Perutz Laboratories, University of Vienna, Vienna, Austria.
authors.forename: Thaddäus
authors.initials: T
authors.lastname: Pfaffenwimmer
authors.affiliation: Department of Chemistry, University of Natural Resources and Applied Biosciences, Vienna, Austria.
authors.forename: Thomas
authors.initials: T
authors.lastname: Dalik
authors.affiliation: Max F. Perutz Laboratories, University of Vienna, Vienna, Austria.
authors.forename: Claudine
authors.initials: C
authors.lastname: Kraft
authors.affiliation: Division of Bioinformatics, Biocenter, Medical University of Innsbruck, Innsbruck, Austria.
authors.forename: Zlatko
authors.initials: Z
authors.lastname: Trajanoski
authors.affiliation: Division of Clinical Biochemistry, ProteinMicroAnalysis Facility, Biocenter, Medical University of Innsbruck, Innsbruck, Austria.
authors.forename: Herbert
authors.initials: H
authors.lastname: Lindner
authors.affiliation: Division of Cell Biology, Biocenter, Medical University of Innsbruck, Innsbruck, Austria.
authors.forename: David
authors.identifier: http://orcid.org/0000-0002-8181-0253
authors.initials: D
authors.lastname: Teis
mesh_terms: D000222:Adaptation, Physiological; D001343:Autophagy / Q000235:genetics*; D002470:Cell Survival / Q000235:genetics; D004705:Endocytosis; D015966:Gene Expression Regulation, Fungal; D053263:Gene Regulatory Networks; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D057146:Multivesicular Bodies / Q000737:chemistry / Q000378:metabolism; D059748:Proteolysis; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D013217:Starvation / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological; D014617:Vacuoles / Q000737:chemistry / Q000378:metabolism*
chemical_list: D029701:Saccharomyces cerevisiae Proteins
keywords: MVB pathway; S. cerevisiae; autophagy; biochemistry; cell biology; endocytosis