title: Biochemistry of storage lesions of red cell and platelet concentrates: A continuous fight implying oxidative/nitrosative/phosphorylative stress and signaling.
abstract: The mechanisms responsible for the reduced lifespan of transfused red blood cells (RBCs) and platelets (PLTs) are still under investigation, however one explanation refers to the detrimental biochemical changes occurring during ex vivo storage of these blood products. A myriad of historical and more recent studies has contributed to advance our understanding of storage lesion. Without any doubts, proteomics had great impact on transfusion medicine by profiling the storage-dependent changes in the total detectable protein pool of both RBCs and PLTs. This review article focuses on the role of oxidative/nitrosative stress in developing RBC and PLT storage lesions, with a special glance at its biochemistry and cross-talk with phosphorylative signal transduction. In this sense, we enlighten the potential contribution of new branches of proteomics in identifying novel points of intervention for the improvement of blood product quality.
authors.affiliation: Department of Ecological and Biological Sciences, University of Tuscia, Viterbo, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Rinalducci
authors.affiliation: Department of Ecological and Biological Sciences, University of Tuscia, Viterbo, Italy. Electronic address: zolla@unitus.it.
authors.forename: Lello
authors.initials: L
authors.lastname: Zolla
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000737:chemistry*; D001793:Blood Preservation / Q000379:methods*; D004906:Erythrocyte Count; D004910:Erythrocyte Membrane / Q000378:metabolism; D017707:Erythrocyte Transfusion / Q000379:methods*; D004912:Erythrocytes / Q000737:chemistry*; D006801:Humans; D009569:Nitric Oxide / Q000737:chemistry; D009584:Nitrogen / Q000737:chemistry; D018384:Oxidative Stress; D010100:Oxygen / Q000737:chemistry; D010766:Phosphorylation; D017713:Platelet Transfusion / Q000379:methods*; D011499:Protein Processing, Post-Translational; D040901:Proteomics / Q000379:methods; D015398:Signal Transduction; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: D013438:Sulfhydryl Compounds; D009569:Nitric Oxide; D009584:Nitrogen; D010100:Oxygen