title: Identification of centromere regions in chromosomes of a unicellular red alga, Cyanidioschyzon merolae.
abstract: To investigate the evolution of centromere architecture in plant cells, it is important to identify centromere regions of primitive algae, such as Cyanidioschyzon merolae. In a previous genome project, in silico analysis predicted an AT-rich region in each chromosome as putative centromere regions. Here, we identified a centromere position in each chromosome by ChIP-on-chip analysis using an anti-CENP-A antibody. The identified centromeres were of the regional type, about 2-3 kb in length and contained no consensus or repeat elements. Centromeres in primitive eukaryotic plant cells may have originated from these regional type centromeres.
authors.affiliation: Genome Research Center, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Kanesaki
authors.affiliation: Chemical Resources Laboratory, Tokyo Institute of Technology, 4259-R1-29 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan.
authors.forename: Sousuke
authors.initials: S
authors.lastname: Imamura
authors.affiliation: Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Motomichi
authors.initials: M
authors.lastname: Matsuzaki
authors.affiliation: Chemical Resources Laboratory, Tokyo Institute of Technology, 4259-R1-29 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan. Electronic address: kntanaka@res.titech.ac.jp.
authors.forename: Kan
authors.initials: K
authors.lastname: Tanaka
mesh_terms: D002503:Centromere / Q000235:genetics* / Q000378:metabolism; D032461:Chromosomes, Plant / Q000235:genetics* / Q000378:metabolism; D019143:Evolution, Molecular; D000461:Rhodophyta / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA
keywords: CENP-A; Centromere; ChIP-chip assay; Cyanidioschyzon merolae; Whole genome tiling array