title: The effects of multifunctional MiR-122-loaded graphene-gold composites on drug-resistant liver cancer.
abstract: BACKGROUND
  Nano drugs have attracted increased attention due to their unique mode of action that offers tumor-inhibiting effects. Therefore, we have previously explored functionalized and drug-loaded graphene-gold nanocomposites that induced cancer cell apoptosis.
  
  
  RESULTS
  In the present study, we developed a combination of monoclonal P-glycoprotein (P-gp) antibodies, folic acid (FA) and miR-122-loaded gold nanoparticles on graphene nanocomposites (GGMPN), which promoted drug-resistant HepG2 cell apoptosis with drug targeting and controlled release properties. We also investigated related apoptosis proteins and apoptosis signal pathways by GGMPN treatment in vitro and in vivo. Moreover, we further demonstrated the inhibition of tumor growth and the apoptosis-inducing effect by means of GGMPN with a semiconductor laser in a xenograft tumor model.
  
  
  CONCLUSIONS
  In conclusion, our results collectively suggested that GGMPN could serve as a novel therapeutic approach to control tumor cell apoptosis and growth.
authors.affiliation: Institute of Stomatology, Nanjing Medical University, Nanjing, 210029, China. xpyywy@126.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing, 210029, China. yaqinzhang@njmu.edu.cn.
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Biomedical Engineering, Nanjing Medical University, Nanjing, 210029, China. sslb_112@hotmail.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.affiliation: Institute of Stomatology, Nanjing Medical University, Nanjing, 210029, China. whmz2002@aliyun.com.
authors.forename: Heming
authors.initials: H
authors.lastname: Wu
authors.affiliation: Jiangnan University Medical School, Wuxi, Jiangsu, 214122, China. kangyj@jiangnan.edu.cn.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Kang
authors.affiliation: Institute of Stomatology, Nanjing Medical University, Nanjing, 210029, China. lmkw180@njmu.edu.cn.
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.affiliation: The State Key Laboratory of Bioelectronics, Department of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China. august555482@hotmail.com.|Maternal and Child Health Institute, Nanjing Maternity and Child Health Care Hospital, Nanjing, 210029, China. august555482@hotmail.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Zeng
authors.affiliation: The State Key Laboratory of Bioelectronics, Department of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China. nyhe1958@163.com.
authors.forename: Nongyue
authors.initials: N
authors.lastname: He
authors.affiliation: Department of Cell Biology, Nanjing Medical University, Nanjing, 210029, China. zhanggen123@126.com.|The State Key Laboratory of Bioelectronics, Department of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China. zhanggen123@126.com.
authors.forename: Gen
authors.initials: G
authors.lastname: Zhang
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D016503:Drug Delivery Systems / Q000379:methods; D019008:Drug Resistance, Neoplasm / Q000187:drug effects*; D005260:Female; D005492:Folic Acid / Q000737:chemistry / Q000494:pharmacology; D006046:Gold / Q000737:chemistry / Q000494:pharmacology; D006108:Graphite / Q000737:chemistry; D056945:Hep G2 Cells / Q000187:drug effects; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000473:pathology; D008819:Mice, Nude; D035683:MicroRNAs / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*; D053761:Nanocomposites / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry; D023041:Xenograft Model Antitumor Assays
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000911:Antibodies, Monoclonal; D000970:Antineoplastic Agents; C531907:MIRN122 microRNA, human; D035683:MicroRNAs; D006046:Gold; D006108:Graphite; D005492:Folic Acid