title: Neuroprotective potential of antioxidant potent fractions from Convolvulus pluricaulis Chois. in 3-nitropropionic acid challenged rats.
abstract: OBJECTIVE
  To isolate the neuroprotective component from Convolvulus pluricaulis Chois. (Convolvulaceae) which can be used as a lead molecule in the treatment of Huntington's disease (HD).
  
  
  METHODS
  The methanolic extract of whole plant was fractionated into four fractions; chloroform, ethyl acetate, n-butanol, and aqueous fraction. The chloroform and ethyl acetate fractions were pooled on the basis of antioxidant activity and TLC profile and charged into silica gel column. Four subfractions were collected from column (FrA, FrB, FrC, and FrD) and further assessed for antioxidant potential by 2,2-diphenyl-1-picrylhydrazyl in vitro assay. 3-Nitropropionic acid (3-NP) was administered to Wister rats for induction of HD. Different fractions were administered for 14 days. Different behavioral alterations were assessed in between study period. Animals were euthanized immediately following the last behavioral session, and biochemical parameters were measured.
  
  
  CONCLUSIONS
  Systemic administration of 3-NP showed marked motor deficits and oxidative damage in rats. Only FrB showed significant antioxidant activity and on further purification gave pure compound (scopoletin). Our study showed that FrB (20 mg/kg) pre-treatment significantly attenuated the loss in body weight, improved the locomotor activity, grip strength, and gait abnormalities. It also has attenuated the increased malondialdehyde and nitrite levels, and restored superoxide dismutase and reduced GSH enzyme activity in the striatum and cortex in 3-NP-treated groups. These results suggest that C. pluricaulis Chois. exhibits a neuroprotective effect by accelerating brain antioxidant defense mechanisms in 3-NP-treated rats.
authors.forename: Mandeep
authors.initials: M
authors.lastname: Kaur
authors.forename: Atish
authors.initials: A
authors.lastname: Prakash
authors.forename: Ajudhia Nath
authors.initials: AN
authors.lastname: Kalia
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001522:Behavior, Animal; D001835:Body Weight; D031254:Convolvulus / Q000737:chemistry*; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006816:Huntington Disease / Q000139:chemically induced / Q000188:drug therapy*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D020258:Neurotoxicity Syndromes / Q000188:drug therapy; D009573:Nitrites / Q000378:metabolism; D009574:Nitro Compounds / Q000009:adverse effects; D011422:Propionates / Q000009:adverse effects; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D018696:Neuroprotective Agents; D009573:Nitrites; D009574:Nitro Compounds; D011422:Propionates; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005978:Glutathione; C015392:3-nitropropionic acid
keywords: 3-Nitropropionic acid; Convolvulus pluricaulis; Huntington disease; Isolation; Oxidative stress; Scopoletin