title: Low programmed cell death 5 expression is a prognostic factor in ovarian cancer.
abstract: BACKGROUND
  Ovarian cancer is a leading gynecological malignancy. We investigated the prognostic value of programmed cell death 5 (PDCD5) in patients with ovarian cancer.
  
  
  METHODS
  Expression levels of PDCD5 mRNA and protein were examined in six ovarian cancer cell lines (SKOV3, CAOV3, ES2, OV1, 3AO, and HOC1A) and one normal ovarian epithelial cell line (T29) using reverse transcription polymerase chain reaction, Western blotting, and flow cytometry. After inducing PDCD5 induction in SKOV3 cells or treating this cell line with taxol or doxorubicin (either alone or combined), apoptosis was measured by Annexin V-FITC/propidium iodide staining. Correlations between PDCD5 protein expression and pathological features, histological grade, FIGO stage, effective cytoreductive surgery, and serum cancer antigen-125 values were evaluated in patients with ovarian cancer.
  
  
  RESULTS
  PDCD5 mRNA and protein expression were downregulated in ovarian cancer cells. Recombinant human PDCD5 increased doxorubicin-induced apoptosis in SKOV3 cells (15.96 ± 2.07%, vs. 3.17 ± 1.45% in controls). In patients with ovarian cancer, PDCD5 expression was inversely correlated with FIGO stage, pathological grade, and patient survival (P < 0.05, R = 0.7139 for survival).
  
  
  CONCLUSIONS
  PDCD5 expression is negatively correlated with disease progression and stage in ovarian cancer. Therefore, measuring PDCD5 expression may be a good method of determining the prognosis of ovarian cancer patients.
authors.forename: Li
authors.initials: L
authors.lastname: Gao
authors.forename: Xue
authors.initials: X
authors.lastname: Ye
authors.forename: Rui-Qiong
authors.initials: RQ
authors.lastname: Ma
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Cheng
authors.forename: Hong-Jing
authors.initials: HJ
authors.lastname: Han
authors.forename: Heng
authors.initials: H
authors.lastname: Cui
authors.forename: Li-Hui
authors.initials: LH
authors.lastname: Wei
authors.affiliation: Gynecology Oncology Center, Peking University People's Hospital, Beijing 100044, China.
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Chang
mesh_terms: D000328:Adult; D000368:Aged; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D011379:Prognosis; D055815:Young Adult
chemical_list: D051017:Apoptosis Regulatory Proteins; D009363:Neoplasm Proteins; C117283:PDCD5 protein, human