title: Biosynthesis of the tetramic acids Sch210971 and Sch210972.
abstract: A biosynthetic pathway to fungal polyketide-nonribosomal peptide natural products, Sch210971 (1a) and Sch210972 (1b) from Hapsidospora irregularis, was characterized by reconstitution and heterologous expression in Fusarium heterosporum. Using genetic, biochemical, and feeding experiments, we show that the incorporated amino acid 4-hydroxyl-4-methyl glutamate (HMG) is synthesized by an aldolase, probably using pyruvate as the precursor.
authors.affiliation: †Department of Medicinal Chemistry, L. S. Skaggs Pharmacy Institute, University of Utah, Salt Lake City, Utah 84112, United States.
authors.forename: Thomas B
authors.initials: TB
authors.lastname: Kakule
authors.affiliation: ‡Department of Biological Engineering, Utah State University, Logan, Utah 84322, United States.
authors.forename: Shuwei
authors.initials: S
authors.lastname: Zhang
authors.affiliation: ‡Department of Biological Engineering, Utah State University, Logan, Utah 84322, United States.
authors.forename: Jixun
authors.initials: J
authors.lastname: Zhan
authors.affiliation: †Department of Medicinal Chemistry, L. S. Skaggs Pharmacy Institute, University of Utah, Salt Lake City, Utah 84112, United States.
authors.forename: Eric W
authors.initials: EW
authors.lastname: Schmidt
mesh_terms: D001669:Biochemical Phenomena; D001688:Biological Products / Q000737:chemistry* / Q000378:metabolism*; D005634:Fructose-Bisphosphate Aldolase / Q000737:chemistry* / Q000378:metabolism; D005658:Fungi / Q000737:chemistry* / Q000378:metabolism*; D005971:Glutamates / Q000138:chemical synthesis* / Q000737:chemistry*; D006576:Heterocyclic Compounds, 4 or More Rings; D015394:Molecular Structure; D010452:Peptide Biosynthesis; D048630:Polyketide Synthases / Q000096:biosynthesis* / Q000737:chemistry*; D061065:Polyketides / Q000737:chemistry* / Q000378:metabolism*; D011760:Pyrrolidinones / Q000737:chemistry* / Q000378:metabolism*
chemical_list: C000601873:4-hydroxyl-4-methyl glutamate; D001688:Biological Products; D005971:Glutamates; D006576:Heterocyclic Compounds, 4 or More Rings; D061065:Polyketides; D011760:Pyrrolidinones; C513451:Sch 210971; C513452:Sch 210972; C009435:tetramic acid; D048630:Polyketide Synthases; D005634:Fructose-Bisphosphate Aldolase