title: Cytotoxicity of commonly used luting cements -An in vitro study.
abstract: The study aimed to 1) evaluate the cytotoxicity of luting cements: Hoffmann's Zinc Phosphate (Hoffmann's ZP), GC Fuji Plus Resin Modified Glass Ionomer (Fuji Plus RMGI) and 3M ESPE RelyX Unicem Resin Cement (RelyX Unicem RC) and 2) test if pre-washing reduces the cements' cytotoxicity. In vitro human gingival fibroblast (HGF) culture model was chosen. The cytotoxicity was evaluated by MTT test, the cell viability -by staining the cells with AO/EB dye mixture. The means±SD of Cell Survival Ratio (CSR%) were compared among different cement types under two testing conditions, with or without cement pre-washing. The CSR%s were compared by ANOVA and linear multiple regression (LMR). Hoffmann's ZPC was less cytotoxic, while Fuji Plus RMGIC and RelyX Unicem RC were more cytotoxic (ANOVA, p<0.001). The type of cement and cement pre-washing jointly explained 90% of cell survival (LMR, p<0.001, adjusted squared R=0.889). The commonly used luting cements such as Hoffmann's ZP, Fuji Plus RMGI and RelyX Unicem RC may have a cytotoxic potential.
authors.affiliation: Institute of Odontology, Faculty of Medicine, Vilnius University.
authors.forename: Rita
authors.initials: R
authors.lastname: Trumpaite-Vanagiene
authors.forename: Virginija
authors.initials: V
authors.lastname: Bukelskiene
authors.forename: Jolanta
authors.initials: J
authors.lastname: Aleksejuniene
authors.forename: Alina
authors.initials: A
authors.lastname: Puriene
authors.forename: Daiva
authors.initials: D
authors.lastname: Baltriukiene
authors.forename: Vygandas
authors.initials: V
authors.lastname: Rutkunas
mesh_terms: D002470:Cell Survival; D003738:Dental Cements / Q000737:chemistry / Q000633:toxicity*; D005347:Fibroblasts / Q000187:drug effects; D005881:Gingiva / Q000166:cytology; D006801:Humans; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D019279:Resin Cements / Q000737:chemistry / Q000633:toxicity; D015036:Zinc Phosphate Cement / Q000737:chemistry / Q000633:toxicity
chemical_list: D003738:Dental Cements; C483701:Rely X Unicem; D019279:Resin Cements; C507315:fuji luting; D015036:Zinc Phosphate Cement