title: Influence of coffee genotype on bioactive compounds and the in vitro capacity to scavenge reactive oxygen and nitrogen species.
abstract: The influence of green coffee genotype on the bioactive compounds and the in vitro antioxidant capacity against the principal reactive oxygen (ROO(•), H2O2, HO(•), and HOCl) and nitrogen (NO(•) and ONOO(-)) species of biological relevance was investigated. This is the first report on the capacity of green coffee to scavenge H2O2, HOCl, and NO(•). Variations in the contents of total chlorogenic acids (22.9-37.9 g/100 g), cinnamoyl-amino acid conjugates (0.03-1.12 g/100 g), trigonelline (3.1-6.7 g/100 g), and caffeine (3.9-11.8 g/100 g) were found. Hydrophilic extracts of Coffea canephora and Coffea kapakata were the most potent scavengers of ROO(•), H2O2, HO(•), NO(•), and ONOO(-) due to their chlorogenic acid contents, which were, on average, 30% higher than those found in Coffea arabica and Coffea racemosa. The results showed that genotype is a determinant characteristic in the bioactive compound contents and consequently in the antioxidant capacity of green coffee.
authors.affiliation: †Faculty of Food Engineering, University of Campinas (UNICAMP), 13083-862 Campinas, São Paulo, Brazil.
authors.forename: Naira Poerner
authors.initials: NP
authors.lastname: Rodrigues
authors.affiliation: §Coffee Center, Agronomic Institute of Campinas (IAC), P.O. Box, 28, 13001-970 Campinas, São Paulo, Brazil.
authors.forename: Terezinha de Jesus Garcia
authors.initials: Tde J
authors.lastname: Salva
authors.affiliation: †Faculty of Food Engineering, University of Campinas (UNICAMP), 13083-862 Campinas, São Paulo, Brazil.
authors.forename: Neura
authors.initials: N
authors.lastname: Bragagnolo
mesh_terms: D002110:Caffeine / Q000737:chemistry; D002726:Chlorogenic Acid / Q000737:chemistry; D040503:Coffea / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D016166:Free Radical Scavengers / Q000737:chemistry*; D005838:Genotype; D010936:Plant Extracts / Q000737:chemistry*; D026361:Reactive Nitrogen Species / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry / Q000145:classification / Q000235:genetics
chemical_list: D016166:Free Radical Scavengers; D010936:Plant Extracts; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D002726:Chlorogenic Acid; D002110:Caffeine
keywords: Rubiaceae; cultivars; germplasm bank; methylxanthines; phenolic compounds; reducing capacity; varieties