title: Pluripotent and Metabolic Features of Two Types of Porcine iPSCs Derived from Defined Mouse and Human ES Cell Culture Conditions.
abstract: The domestic pig is an excellent animal model for stem cell research and clinical medicine. There is still no suitable culture condition to generate authentic porcine embryonic stem cells (pESCs) and high quality porcine induced pluripotent stem cells (piPSCs). In this study, we found that culture conditions affected pluripotent and metabolic features of piPSCs. Using defined human embryonic stem cell (hESC) and mouse ESC (mESC) culture conditions, we generated two types of piPSCs, one of which was morphologically similar to hESCs (here called hpiPSCs), the other resembled mESCs (here called mpiPSCs). Transcriptome analysis and signaling pathway inhibition results suggested that mpiPSCs shared more of mESC signaling pathways, such as the BMP pathway and JAK/STAT pathway and hpiPSCs shared more hESC signaling pathways, such as the FGF pathway. Importantly, the mpiPSCs performed embryonic chimera incorporation more efficiently than the hpiPSCs did. In addition, the mpiPSCs showed mitochondrial features of naive ESCs and lipid droplets accumulation. These evidences may facilitate understanding of the gene regulation network and metabolism in piPSCs and promote derivation of bona fide pESCs for translational medicine.
authors.affiliation: State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.
authors.forename: Yangli
authors.initials: Y
authors.lastname: Pei
authors.affiliation: State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhong
authors.affiliation: State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.
authors.forename: Bingqiang
authors.initials: B
authors.lastname: Wen
authors.affiliation: College of Animal Science and Technology, Beijing University of Agriculture, Beijing, China.
authors.forename: Suying
authors.initials: S
authors.lastname: Cao
authors.affiliation: State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.
authors.forename: Jianyong
authors.initials: J
authors.lastname: Han
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology; D047108:Embryonic Development; D005260:Female; D020869:Gene Expression Profiling; D000066449:Human Embryonic Stem Cells / Q000166:cytology*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D066292:Lipid Droplets / Q000378:metabolism / Q000648:ultrastructure; D051379:Mice; D008928:Mitochondria / Q000378:metabolism / Q000648:ultrastructure; D000066450:Mouse Embryonic Stem Cells / Q000166:cytology*; D010312:Parthenogenesis; D015398:Signal Transduction; D034421:Sus scrofa; D059467:Transcriptome / Q000235:genetics