title: Label-free in vivo imaging of peripheral nerve by multispectral photoacoustic tomography.
abstract: Unintentional surgical damage to nerves is mainly due to poor visualization of nerve tissue relative to adjacent structures. Multispectral photoacoustic tomography can provide chemical information with specificity and ultrasonic spatial resolution with centimeter imaging depth, making it a potential tool for noninvasive neural imaging. To implement this label-free imaging approach, a multispectral photoacoustic tomography platform was built. Imaging depth and spatial resolution were characterized. In vivo imaging of the femoral nerve that is 2 mm deep in a nude mouse was performed. Through multivariate curve resolution analysis, the femoral nerve was discriminated from the femoral artery and chemical maps of their spatial distributions were generated.
authors.affiliation: Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, 47907, USA.
authors.forename: Rui
authors.initials: R
authors.lastname: Li
authors.affiliation: Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, 47907, USA.
authors.forename: Evan
authors.initials: E
authors.lastname: Phillips
authors.affiliation: Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, 47907, USA.
authors.forename: Pu
authors.initials: P
authors.lastname: Wang
authors.affiliation: Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, 47907, USA. cgoergen@purdue.edu.
authors.forename: Craig J
authors.initials: CJ
authors.lastname: Goergen
authors.affiliation: Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, 47907, USA. jcheng@purdue.edu.|Department of Chemistry, Purdue University, West Lafayette, IN, 47907, USA. jcheng@purdue.edu.
authors.forename: Ji-Xin
authors.initials: JX
authors.lastname: Cheng
mesh_terms: D000818:Animals; D005263:Femoral Artery / Q000294:innervation; D005267:Femoral Nerve / Q000000981:diagnostic imaging*; D051379:Mice; D008819:Mice, Nude; D019047:Phantoms, Imaging; D061088:Photoacoustic Techniques / Q000379:methods*; D059629:Signal-To-Noise Ratio; D014054:Tomography / Q000379:methods*; D014463:Ultrasonography
keywords: in vivo; multivariate curve resolution; nerve; photoacoustic tomography; spectroscopy