title: Circulation of prions within dust on a scrapie affected farm.
abstract: Prion diseases are fatal neurological disorders that affect humans and animals. Scrapie of sheep/goats and Chronic Wasting Disease (CWD) of deer/elk are contagious prion diseases where environmental reservoirs have a direct link to the transmission of disease. Using protein misfolding cyclic amplification we demonstrate that scrapie PrP(Sc) can be detected within circulating dusts that are present on a farm that is naturally contaminated with sheep scrapie. The presence of infectious scrapie within airborne dusts may represent a possible route of infection and illustrates the difficulties that may be associated with the effective decontamination of such scrapie affected premises.
authors.affiliation: School of Veterinary Medicine and Science, The University of Nottingham, Sutton Bonington, Loughborough, Leicestershire, LE12 5RD, UK. kevin.gough@nottingham.ac.uk.
authors.forename: Kevin C
authors.initials: KC
authors.lastname: Gough
authors.affiliation: ADAS UK, School of Veterinary Medicine and Science, The University of Nottingham, Sutton Bonington, Loughborough, Leicestershire, LE12 5RD, UK. claire.baker@adas.co.uk.
authors.forename: Claire A
authors.initials: CA
authors.lastname: Baker
authors.affiliation: Animal and Plant Health Agency, Woodham Lane, New Haw, Addlestone, Surrey, KT15 3NB, UK. hugh.simmons@apha.gsi.gov.uk.
authors.forename: Hugh A
authors.initials: HA
authors.lastname: Simmons
authors.affiliation: Animal and Plant Health Agency, Woodham Lane, New Haw, Addlestone, Surrey, KT15 3NB, UK. steve.hawkins@apha.gsi.gov.uk.
authors.forename: Steve A
authors.initials: SA
authors.lastname: Hawkins
authors.affiliation: ADAS UK, School of Veterinary Medicine and Science, The University of Nottingham, Sutton Bonington, Loughborough, Leicestershire, LE12 5RD, UK. ben.maddison@adas.co.uk.
authors.forename: Ben C
authors.initials: BC
authors.lastname: Maddison
mesh_terms: D000818:Animals; D004391:Dust / Q000032:analysis*; D004739:England; D018620:PrPSc Proteins / Q000032:analysis*; D012608:Scrapie / Q000453:epidemiology* / Q000209:etiology; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology* / Q000209:etiology
chemical_list: D004391:Dust; D018620:PrPSc Proteins