title: Analysis of chemokines and receptors expression profile in the myelin mutant taiep rat.
abstract: Taiep rat has a failure in myelination and remyelination processes leading to a state of hypomyelination throughout its life. Chemokines, which are known to play a role in inflammation, are also involved in the remyelination process. We aimed to demonstrate that remyelination-stimulating factors are altered in the brainstem of 1- and 6-month-old taiep rats. We used a Rat RT(2) Profiler PCR Array to assess mRNA expression of 84 genes coding for cytokines, chemokines, and their receptors. We also evaluated protein levels of CCL2, CCR1, CCR2, CCL5, CCR5, CCR8, CXCL1, CXCR2, CXCR4, FGF2, and VEGFA by ELISA. Sprague-Dawley rats were used as a control. PCR Array procedure showed that proinflammatory cytokines were not upregulated in the taiep rat. In contrast, some mRNA levels of beta and alpha chemokines were upregulated in 1-month-old rats, but CXCR4 was downregulated at their 6 months of age. ELISA results showed that CXCL1, CCL2, CCR2, CCR5, CCR8, and CXCR4 protein levels were decreased in brainstem at the age of 6 months. These results suggest the presence of a chronic neuroinflammation process with deficiency of remyelination-stimulating factors (CXCL1, CXCR2, and CXCR4), which might account for the demyelination in the taiep rat.
authors.affiliation: Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, 14 Sur y Avenida San Claudio, 72570 Puebla, PUE, Mexico.
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Soto-Rodriguez
authors.affiliation: Laboratorio de Medicina Genómica, Hospital Regional 1° de Octubre, ISSSTE, Avenida Instituto Politécnico Nacional No. 1669, 07760 México, DF, Mexico.
authors.forename: Juan-Antonio
authors.initials: JA
authors.lastname: Gonzalez-Barrios
authors.affiliation: Departamento de Fisiología, Biofísica y Neurociencias, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Apartado Postal 14-740, 07000 México, DF, Mexico.
authors.forename: Daniel
authors.initials: D
authors.lastname: Martinez-Fong
authors.affiliation: Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, 14 Sur y Avenida San Claudio, 72570 Puebla, PUE, Mexico.
authors.forename: Victor-Manuel
authors.initials: VM
authors.lastname: Blanco-Alvarez
authors.affiliation: Instituto de Fisiología, Benemérita Universidad Autónoma de Puebla, 14 Sur 6301, 72560 Puebla, PUE, Mexico.
authors.forename: Jose R
authors.initials: JR
authors.lastname: Eguibar
authors.affiliation: Instituto de Fisiología, Benemérita Universidad Autónoma de Puebla, 14 Sur 6301, 72560 Puebla, PUE, Mexico.
authors.forename: Araceli
authors.initials: A
authors.lastname: Ugarte
authors.affiliation: Laboratorio de Genómica de Celomados, Grupo de Microbiología y Genética de la Escuela de Biología, Universidad Industrial de Santander, Apartado Postal 680002, Bucaramanga, Colombia.
authors.forename: Francisco
authors.initials: F
authors.lastname: Martinez-Perez
authors.affiliation: Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, 14 Sur y Avenida San Claudio, 72570 Puebla, PUE, Mexico.
authors.forename: Eduardo
authors.identifier: 0000-0003-0377-0943
authors.initials: E
authors.lastname: Brambila
authors.affiliation: Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, 14 Sur y Avenida San Claudio, 72570 Puebla, PUE, Mexico.
authors.forename: Lourdes Millán-Perez
authors.initials: LM
authors.lastname: Peña
authors.affiliation: Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, 14 Sur y Avenida San Claudio, 72570 Puebla, PUE, Mexico.
authors.forename: Nidia-Gary
authors.initials: NG
authors.lastname: Pazos-Salazar
authors.affiliation: Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, 14 Sur y Avenida San Claudio, 72570 Puebla, PUE, Mexico.
authors.forename: Maricela
authors.initials: M
authors.lastname: Torres-Soto
authors.affiliation: Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, 14 Sur y Avenida San Claudio, 72570 Puebla, PUE, Mexico.
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Garcia-Robles
authors.affiliation: Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, 14 Sur y Avenida San Claudio, 72570 Puebla, PUE, Mexico.
authors.forename: Constantino
authors.initials: C
authors.lastname: Tomas-Sanchez
authors.affiliation: Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, 14 Sur y Avenida San Claudio, 72570 Puebla, PUE, Mexico.
authors.forename: Bertha Alicia
authors.identifier: 0000-0002-8041-9404
authors.initials: BA
authors.lastname: Leon-Chavez
mesh_terms: D000818:Animals; D018925:Chemokines / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D004797:Enzyme-Linked Immunosorbent Assay; D008856:Microscopy, Fluorescence; D009186:Myelin Sheath / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D055647:Rats, Transgenic; D019707:Receptors, Chemokine / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome; D015854:Up-Regulation
chemical_list: D018925:Chemokines; D012333:RNA, Messenger; D019707:Receptors, Chemokine