title: Influence of acrylamide on the gastric mucosa of adult albino rats and the possible protective role of rosemary.
abstract: Acrylamide is a common chemical found in heated starchy foods especially potato products. We investigated, for the first time, the effect of acrylamide, alone or with rosemary, on the structure of gastric mucosa of adult male albino rats. Stomach sections were examined using light and scanning electron microscopy. Quantitative immmunohistochemical assessments of the expression of caspase-3, inducible NO synthase (iNOS) and epidermal growth factor receptor (EPGR) were performed. Our results showed that acrylamide produced mucosal erosions and depletion of the protective surface mucus together with widespread inflammatory infiltration. In addition, there was significantly increased expression of caspase-3 and iNOS and weak expression of EPGR. Rosemary exerted a protective effect against acrylamide-induced gastric toxicity via reducing oxidative stress, apoptosis and inflammation as well as accelerating the healing process. The results of this work add to the known toxic effects of acrylamide and provide a new insight into the possible use of rosemary to ameliorate these effects.
authors.affiliation: Anatomy and Embryology Department, Faculty of Medicine, Menoufia University, Egypt. Electronic address: abeermehi@gmail.com.
authors.forename: Abeer E
authors.initials: AE
authors.lastname: El-Mehi
authors.affiliation: Anatomy and Embryology Department, Faculty of Medicine, Menoufia University, Egypt.
authors.forename: Neveen M
authors.initials: NM
authors.lastname: El-Sherif
mesh_terms: D020106:Acrylamide / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000096:biosynthesis / Q000235:genetics; D066246:ErbB Receptors / Q000096:biosynthesis / Q000235:genetics; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis / Q000235:genetics; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D051381:Rats; D027542:Rosmarinus / Q000737:chemistry; D013270:Stomach / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics
chemical_list: D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D020106:Acrylamide; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; C483803:Egfr protein, rat; D066246:ErbB Receptors; D053148:Caspase 3
keywords: Acrylamide; Histopathology; Immunohistochemistry; Rosemary; Stomach