title: Interaction of Proteasomes and Complement C3, Assay of Antisecretory Factor in Blood.
abstract: Antisecretory factor (AF) is a protein complex which inhibits inflammation and regulates fluid transport. In this article, two new immunoassays (ELISA) are developed. The first ELISA establishes a 26S proteasome concentration of 0.41±0.03 μg/mL in normal plasma; the second ELISA discloses the binding of proteasomes to complement factor C3. The latter test values increased about tenfold following intake of processed cereals, paralleling with the old AF ELISA. The proteasome/C3 complex is purified and shown to expose hidden antisecretory peptide sequence and contain the inactive C3c protein. These findings might explain the antisecretory and anti-inflammatory effect during AF complex formation.
authors.affiliation: a Department of Infectious Diseases , Institute of Biomedicine, Gothenburg University , Gothenburg , Sweden.
authors.forename: Ivar
authors.initials: I
authors.lastname: Lönnroth
authors.affiliation: a Department of Infectious Diseases , Institute of Biomedicine, Gothenburg University , Gothenburg , Sweden.|b Clinical Microbiology , Sahlgrenska University Hospital , Gothenburg , Sweden.
authors.forename: Merna
authors.initials: M
authors.lastname: Oshalim
authors.affiliation: a Department of Infectious Diseases , Institute of Biomedicine, Gothenburg University , Gothenburg , Sweden.|b Clinical Microbiology , Sahlgrenska University Hospital , Gothenburg , Sweden.
authors.forename: Stefan
authors.initials: S
authors.lastname: Lange
authors.affiliation: a Department of Infectious Diseases , Institute of Biomedicine, Gothenburg University , Gothenburg , Sweden.|b Clinical Microbiology , Sahlgrenska University Hospital , Gothenburg , Sweden.
authors.forename: Ewa
authors.initials: E
authors.lastname: Johansson
mesh_terms: D001665:Binding Sites; D003176:Complement C3 / Q000378:metabolism*; D004040:Dietary Carbohydrates / Q000008:administration & dosage / Q000097:blood / Q000276:immunology; D002523:Edible Grain / Q000276:immunology / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D015870:Gene Expression; D006801:Humans; D009479:Neuropeptides / Q000097:blood* / Q000276:immunology; D046988:Proteasome Endopeptidase Complex / Q000097:blood* / Q000276:immunology; D011485:Protein Binding; D059748:Proteolysis
chemical_list: D003176:Complement C3; D004040:Dietary Carbohydrates; D009479:Neuropeptides; C049628:antisecretory factor; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease
keywords: antisecretory factor; cereals; circulating proteasomes; complement; inflammation