title: 1α,25-dihydroxyvitamin D₃ counteracts the effects of cigarette smoke in airway epithelial cells.
abstract: Cigarette smoke extracts (CSE) alter calpain-1 expression via ERK signaling pathway in bronchial epithelial cells. 1α,25-dihydroxyvitamin D3 (1,25D3) inhibits cigarette smoke-induced epithelial barrier disruption. This study was aimed to explore whether the 1,25D3 counteracted the CSE effects in a human bronchial epithelial cell line (16HBE). In particular, transepithelial electrical resistance (TER) and permeability, expression and distribution of E-cadherin and β-catenin, calpain-1 expression, and ERK phosphorylation were assessed in the CSE-stimulated 16HBE cells. The CSE induced the ERK phosphorylation, improved the calpain-1 expression, increased the distribution anomalies and the cleaving of E-cadherin and β-catenin, and resulted in the TER reduction and the permeability increase. The 1,25D3 reduced these pathological changes. The 1,25D3 mediated effects were associated with a reduced ERK phosphorylation. In conclusion, the present study provides compelling evidences that the 1,25D3 may be considered a possible valid therapeutic option in controlling the cigarette smoke-induced epithelial barrier disruption.
authors.affiliation: Department of Respiratory, Chronic Airways Diseases Laboratory, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Ruhui
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Respiratory, Chronic Airways Diseases Laboratory, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Haijin
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Respiratory, Chronic Airways Diseases Laboratory, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Hangming
authors.initials: H
authors.lastname: Dong
authors.affiliation: School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Zou
authors.affiliation: Department of Respiratory, Chronic Airways Diseases Laboratory, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. Electronic address: caishaox@fimmu.com.
authors.forename: Shaoxi
authors.initials: S
authors.lastname: Cai
mesh_terms: D015153:Blotting, Western; D015820:Cadherins / Q000276:immunology; D002117:Calcitriol / Q000494:pharmacology*; D002154:Calpain / Q000276:immunology; D002460:Cell Line; D017097:Electric Impedance; D004847:Epithelial Cells / Q000276:immunology; D006801:Humans; D020935:MAP Kinase Signaling System / Q000276:immunology; D008856:Microscopy, Fluorescence; D020545:Respiratory Mucosa / Q000187:drug effects* / Q000276:immunology*; D012906:Smoke / Q000009:adverse effects*; D014026:Nicotiana / Q000009:adverse effects*; D051176:beta Catenin / Q000276:immunology
chemical_list: D015820:Cadherins; D012906:Smoke; D051176:beta Catenin; D002154:Calpain; D002117:Calcitriol
keywords: 1α,25-Dihydroxyvitamin D(3); Calpain-1; Cigarette smoke extracts; E-cadherin; ERK