title: CD200+ and CD200- macrophages accumulated in ischemic lesions of rat brain: the two populations cannot be classified as either M1 or M2 macrophages.
abstract: Two types of macrophages in lesion core of rat stroke model were identified according to NG2 chondroitin sulfate proteoglycan (NG2) and CD200 expression. NG2(+) macrophages were CD200(-), and vice versa. NG2(-) macrophages expressed two splice variants of CD200 that are CD200L and CD200S. CD200(+) macrophages expressed CD8, CD68, CD163, CCL2, inducible nitric oxide synthase, interleukin-1β, Toll-like receptor 4 and transforming growth factor β, whilst NG2(+) cells expressed a costimulatory factor CD86. Both cell types expressed insulin-like growth factor 1 and CD200R. These results demonstrate that the two macrophage types cannot be classified as either M1 or M2.
authors.affiliation: Department of Neurosurgery, Graduate School of Medicine, Ehime University, Japan.
authors.forename: Shirabe
authors.initials: S
authors.lastname: Matsumoto
authors.affiliation: Department of Molecular and Cellular Physiology, Graduate School of Medicine, Ehime University, Japan. Electronic address: jtanaka@m.ehime-u.ac.jp.
authors.forename: Junya
authors.initials: J
authors.lastname: Tanaka
authors.affiliation: Department of Molecular and Cellular Physiology, Graduate School of Medicine, Ehime University, Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Yano
authors.affiliation: Department of Molecular and Cellular Physiology, Graduate School of Medicine, Ehime University, Japan; Center for Advanced Research and Education, Asahikawa Medical University, Japan.
authors.forename: Hisaaki
authors.initials: H
authors.lastname: Takahashi
authors.affiliation: Department of Molecular and Cellular Physiology, Graduate School of Medicine, Ehime University, Japan; Department of Legal Medicine, Graduate School of Medicine/Faculty of Medicine, Osaka University, Japan.
authors.forename: Kana
authors.initials: K
authors.lastname: Sugimoto
authors.affiliation: Department of Neurosurgery, Graduate School of Medicine, Ehime University, Japan.
authors.forename: Shiro
authors.initials: S
authors.lastname: Ohue
authors.affiliation: Department of Neurosurgery, Graduate School of Medicine, Ehime University, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Inoue
authors.affiliation: Department of Molecular and Cellular Physiology, Graduate School of Medicine, Ehime University, Japan.
authors.forename: Hitomi
authors.initials: H
authors.lastname: Aono
authors.affiliation: Department of Neurosurgery, Graduate School of Medicine, Ehime University, Japan.
authors.forename: Akari
authors.initials: A
authors.lastname: Kusakawa
authors.affiliation: Department of Neurosurgery, Graduate School of Medicine, Ehime University, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Watanabe
authors.affiliation: Department of Regeneration of Community Medicine, Graduate School of Medicine, Ehime University, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kumon
authors.affiliation: Department of Neurosurgery, Graduate School of Medicine, Ehime University, Japan.
authors.forename: Takanori
authors.initials: T
authors.lastname: Ohnishi
mesh_terms: D000818:Animals; D000941:Antigens / Q000378:metabolism; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism*; D016026:Bone Marrow Transplantation; D001921:Brain / Q000378:metabolism / Q000473:pathology*; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002452:Cell Count; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000502:physiology*; D020244:Infarction, Middle Cerebral Artery / Q000473:pathology* / Q000601:surgery; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D019394:Ki-67 Antigen / Q000378:metabolism; D008264:Macrophages / Q000145:classification* / Q000378:metabolism*; D008297:Male; D008840:Microfilament Proteins / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D011509:Proteoglycans / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D055647:Rats, Transgenic; D017208:Rats, Wistar; D051197:Toll-Like Receptor 4 / Q000378:metabolism; D000099216:Chondroitin Sulfate Proteoglycan 4
chemical_list: D000941:Antigens; D015703:Antigens, CD; D002135:Calcium-Binding Proteins; D016207:Cytokines; D007334:Insulin-Like Growth Factor I; D019394:Ki-67 Antigen; D008840:Microfilament Proteins; D052247:Nitric Oxide Synthase Type II; D011509:Proteoglycans; D051197:Toll-Like Receptor 4; D000099216:Chondroitin Sulfate Proteoglycan 4; C100745:Aif1 protein, rat; C495347:Tlr4 protein, rat; C548904:insulin-like growth factor-1, rat; C042739:antigens, CD200
keywords: Alternative; CD68; CD86; Ki67; NG2; TGFβ; TLR4; iNOS