title: Ultrasensitive electrochemical immunoassay of proteins based on in situ duple amplification of gold nanoparticle biolabel signals.
abstract: An electrochemical sandwich immunoassay method that can be sensitive to a few protein molecules (human immunoglobulin G or human prostate-specific antigen) is reported, based on HAuCl4-NH2OH redox reaction to enlarge the size of second antibody labeled gold nanoparticles and in situ microliter-droplet anodic stripping voltammetry analysis with enhanced cathodic preconcentration of gold.
authors.affiliation: Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (MOE of China), National & Local Joint Engineering Laboratory for New Petro-chemical Materials and Fine Utilization of Resources, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China. xieqj@hunnu.edu.cn.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Qin
authors.forename: Aigui
authors.initials: A
authors.lastname: Xu
authors.forename: Ling
authors.initials: L
authors.lastname: Liu
authors.forename: Wenfang
authors.initials: W
authors.lastname: Deng
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
authors.forename: Yueming
authors.initials: Y
authors.lastname: Tan
authors.forename: Yingchun
authors.initials: Y
authors.lastname: Fu
authors.forename: Qingji
authors.initials: Q
authors.lastname: Xie
authors.forename: Shouzhuo
authors.initials: S
authors.lastname: Yao
mesh_terms: D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D004563:Electrochemistry; D006046:Gold / Q000737:chemistry*; D007118:Immunoassay; D007074:Immunoglobulin G / Q000032:analysis* / Q000276:immunology; D007610:Kallikreins / Q000032:analysis* / Q000276:immunology; D053768:Metal Nanoparticles / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry; D017430:Prostate-Specific Antigen / Q000032:analysis* / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: D000906:Antibodies; D007074:Immunoglobulin G; D037742:Nanotubes, Carbon; D012710:Serum Albumin, Bovine; D006046:Gold; C549870:KLK3 protein, human; D007610:Kallikreins; D017430:Prostate-Specific Antigen