title: Cardioprotective effect of linseed oil against isoproterenol-induced myocardial infarction in Wistar rats: a biochemical and electrocardiographic study.
abstract: The present study was designed to evaluate the cardioprotective effect of Tunisian flaxseed oil (Linum usitatissimum) against isoproterenol-induced myocardial infarction in rats by studying hypertensive and cardiac damage markers especially electrocardiographic changes and troponin T serum level. In vitro, the extracted oil showed an important inhibition of angiotensin converting enzyme (ACE) with an IC50 = 85.96 μg/ml. According to chemical analysis, this extract is composed essentially of alpha linolenic acid (ALA), an n-3 polyunsaturated fatty acid (58.59 %). Male rats were randomly divided into three groups, namely control (C), isoproterenol (ISO), and isoproterenol-treated group with flaxseed oil (FO + ISO). Isoproterenol injection showed changes in ECG pattern, including ST-segment elevation (diagnostic of myocardial infarction), increase in the serum levels of Troponin T and cardiac injury markers (creatine kinase-MB (CK-MB), lactate dehydrogenase (LDH), alkaline phosphatase (ALP), aspartate transaminase (AST), and alanine transaminase (ALT)). However, Linum oil pre-co-treatment prevented almost all the parameters isoproterenol-induced myocardial infarction in rats. Results of the present study proved that flaxseed oil has a significant effect by heart protection against isoproterenol-induced myocardial infarction through beneficial effect of the important fraction of ALA.
authors.affiliation: Laboratory of Animal Ecophysiology, Faculty of Sciences of Sfax, University of Sfax, P.O. Box 95, 3052, Sfax, Tunisia.
authors.forename: Amal
authors.initials: A
authors.lastname: Derbali
authors.forename: Kais
authors.initials: K
authors.lastname: Mnafgui
authors.forename: Marwa
authors.initials: M
authors.lastname: Affes
authors.forename: Fatma
authors.initials: F
authors.lastname: Derbali
authors.forename: Raouf
authors.initials: R
authors.lastname: Hajji
authors.forename: Neji
authors.initials: N
authors.lastname: Gharsallah
authors.forename: Noureddine
authors.initials: N
authors.lastname: Allouche
authors.forename: Abdelfattah
authors.initials: A
authors.lastname: El Feki
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002316:Cardiotonic Agents / Q000494:pharmacology*; D004562:Electrocardiography; D004798:Enzymes / Q000097:blood; D005227:Fatty Acids / Q000032:analysis; D019597:Flax / Q000737:chemistry; D007545:Isoproterenol / Q000009:adverse effects*; D008043:Linseed Oil / Q000737:chemistry* / Q000494:pharmacology*; D008297:Male; D009203:Myocardial Infarction / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D009929:Organ Size / Q000187:drug effects; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D017208:Rats, Wistar; D020107:Troponin T / Q000378:metabolism
chemical_list: D002316:Cardiotonic Agents; D004798:Enzymes; D005227:Fatty Acids; D010936:Plant Extracts; D020107:Troponin T; D008043:Linseed Oil; D007703:Peptidyl-Dipeptidase A; D007545:Isoproterenol