title: A proliferation-inducing ligand sustains the proliferation of human naïve (CD27⁻) B cells and mediates their differentiation into long-lived plasma cells in vitro via transmembrane activator and calcium modulator and cyclophilin ligand interactor and B-cell mature antigen.
abstract: Long-lived plasma cells (PCs) contribute to humoral immunity through an undefined mechanism. Memory B cells, but not human naïve B cells, can be induced to differentiate into long-lived PCs in vitro. Because evidence links a proliferation-inducing ligand (APRIL), a tumor necrosis factor family member, to the ability of bone marrow to mediate long-term PC survival, we reasoned that APRIL influences the proliferation and differentiation of naïve B cells. We describe here the development of a simple cell culture system that allowed us to show that APRIL sustained the proliferation of naïve human B cells and induced them to differentiate into long-lived PCs. Blocking the transmembrane activator and calcium modulator and cyclophilin ligand interactor or B-cell mature antigen shows they were required for the differentiation of naïve B cells into long-lived PCs in vitro. Our in vitro culture system will reveal new insights into the biology of long-lived PCs.
authors.affiliation: Department of Transplant Immunology, Nagoya University School of Medicine, Nagoya, Japan.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Matsuda
authors.affiliation: Department of Transplant Immunology, Nagoya University School of Medicine, Nagoya, Japan. Electronic address: m-haneda@med.nagoya-u.ac.jp.
authors.forename: Masataka
authors.initials: M
authors.lastname: Haneda
authors.affiliation: Department of Biochemistry, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Kadomatsu
authors.affiliation: Department of Transplant Immunology, Nagoya University School of Medicine, Nagoya, Japan.
authors.forename: Takaaki
authors.initials: T
authors.lastname: Kobayashi
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D053301:B-Cell Maturation Antigen / Q000037:antagonists & inhibitors / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D053303:Transmembrane Activator and CAML Interactor Protein / Q000037:antagonists & inhibitors / Q000276:immunology*; D053300:Tumor Necrosis Factor Ligand Superfamily Member 13 / Q000494:pharmacology*; D055815:Young Adult
chemical_list: D000911:Antibodies, Monoclonal; D053301:B-Cell Maturation Antigen; C086966:TNFRSF17 protein, human; C114631:TNFSF13 protein, human; D053303:Transmembrane Activator and CAML Interactor Protein; D053300:Tumor Necrosis Factor Ligand Superfamily Member 13
keywords: A proliferation-inducing ligand; B-cell mature antigen; Long-lived plasma cell; Naïve B cell; Transmembrane activator and calcium modulator and cyclophilin ligand interactor