title: [Development of methods for rapid test of staphylococcal enterotoxin A in food].
abstract: Noninstrumental methods of qualitative rapid test for detection of staphylococcal enterotoxin A (SEA) in milk foods sample and brothof using immunochromatography (IC) and dot-assay has been developed. Monoclonal antibodies to SEA with colloidal gold forimmunochromatography; monoclonal antibodies to SEA with colloidal gold or biotinylated monoclonal antibodies and streptavidin-peroxidase conjugate for dot-assay were used to visualize the results. The detection limits, ng/mL: 10 (IC), 20 (dot-assay with antibody-colloidal gold), 10 (dot-assay with STR-HRP), 4 (ELISA). Time of assay, min: 25 (IC), 60 (dot-assay with antibody-colloidal gold), 70 (dot-assay with STR-HRPO, 150 (ELISA).
authors.forename: I A
authors.initials: IA
authors.lastname: Liubavina
authors.forename: F A
authors.initials: FA
authors.lastname: Brovko
authors.forename: T I
authors.initials: TI
authors.lastname: Valiakina
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Vertiev
authors.forename: E V
authors.initials: EV
authors.lastname: Grishin
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D004768:Enterotoxins / Q000737:chemistry / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005504:Food Analysis / Q000379:methods*; D017979:Gold Colloid / Q000737:chemistry; D006801:Humans; D008461:Meat Products / Q000382:microbiology*; D008892:Milk / Q000382:microbiology*; D019809:Streptavidin / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D004768:Enterotoxins; D017979:Gold Colloid; C026066:enterotoxin A, Staphylococcal; D019809:Streptavidin