title: Impact of MBL and MASP-2 gene polymorphism and its interaction on susceptibility to tuberculosis.
abstract: BACKGROUND
  Mannose-binding lectin (MBL) and MBL-associated serine proteases 2 (MASP-2) are important proteins in the lectin pathway of the immune system. Polymorphism of MBL and MASP-2 genes may affect the serum concentration of MBL and MASP-2. This study explores the association between MBL and MASP-2 gene polymorphism and their interactions and the susceptibility to tuberculosis (TB).
  
  
  METHODS
  A total of 503 patients with TB and 419 healthy controls were recruited to participate in this case-control study. PCR-SSP technology was applied to genotype rs7096206 of MBL genes and rs2273346 and rs6695096 of MASP-2 genes. Demographic data and some exposure information were also obtained from study participants. Unconditional logistic regression analysis was used to identify association between the various factors and TB whilst Marginal Structural Linear Odds Models were used to estimate the interactions.
  
  
  RESULTS
  Both genotype GC at rs7096206 of MBL genes and genotype TC at rs2273346 and rs6695096 of MASP-2 genes were more prevalent in the TB patient group than the healthy control group (P<0.05, OR 1.393, 1.302 and 1.426 respectively). The relative excess risk of interaction (RERI) between rs7096206 of MBL genes and rs2273346 and rs6695096 of MASP-2 genes was 0.897 (95% CI: 0.282, 1.513) and 1.142 (95% CI: 0.755, 1.530) respectively (P<0.05).
  
  
  CONCLUSIONS
  Polymorphisms of MBL (rs7096206) and MASP-2 (rs2273346 and rs6695096) were associated with the susceptibility of TB, and there were gene-gene interactions among them.
authors.affiliation: Department of Epidemiology and Health Statistics, School of Public Health, Central South University, Changsha, Hunan, 410008, PR China. 121444639@qq.com.|Hunan Children's Hospital, Ziyuan RD 86, Changsha, Hunan, 410007, PR China. 121444639@qq.com.
authors.forename: Mengshi
authors.initials: M
authors.lastname: Chen
authors.affiliation: Department of Epidemiology and Health Statistics, School of Public Health, Central South University, Changsha, Hunan, 410008, PR China. liangying@bit.edu.cn.|School of Public Health, Xinjiang Medical University, Urumqi, Xijiang, 830054, PR China. liangying@bit.edu.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Department of Nursing, Shaoyang Medical College, Shaoyang, Hunan, 422000, PR China. lwf820126@163.com.
authors.forename: Wufei
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Epidemiology and Health Statistics, School of Public Health, Central South University, Changsha, Hunan, 410008, PR China. wangmian129@163.com.
authors.forename: Mian
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Epidemiology and Health Statistics, School of Public Health, Central South University, Changsha, Hunan, 410008, PR China. 371693893@qq.com.|Beijing Center for Diseases Prevention and Control, Beijing, 100013, PR China. 371693893@qq.com.
authors.forename: Li
authors.initials: L
authors.lastname: Hu
authors.affiliation: Department of Epidemiology and Health Statistics, School of Public Health, Central South University, Changsha, Hunan, 410008, PR China. BAbuaku@noguchi.mimcom.org.|Department of Epidemiology, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, PO Box LG581, Legon, Accra, Ghana. BAbuaku@noguchi.mimcom.org.
authors.forename: Benjamin Kwaku
authors.initials: BK
authors.lastname: Abuaku
authors.affiliation: Department of Epidemiology and Health Statistics, School of Public Health, Central South University, Changsha, Hunan, 410008, PR China. monaxinhuang@qq.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Huang
authors.affiliation: Department of Epidemiology and Health Statistics, School of Public Health, Central South University, Changsha, Hunan, 410008, PR China. tanhz99@qq.com.
authors.forename: Hongzhuan
authors.initials: H
authors.lastname: Tan
authors.affiliation: Department of Epidemiology and Health Statistics, School of Public Health, Central South University, Changsha, Hunan, 410008, PR China. swwen@ohri.ca.|Department of Obstetrics & Gynecology, University of Ottawa, The Ottawa Hospital, 501 Smyth Road, Ottawa, Ontario, Canada. swwen@ohri.ca.
authors.forename: Shi Wu
authors.initials: SW
authors.lastname: Wen
mesh_terms: D016022:Case-Control Studies; D002681:China; D005260:Female; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D016014:Linear Models; D016015:Logistic Models; D008297:Male; D037601:Mannose-Binding Lectin / Q000235:genetics*; D050606:Mannose-Binding Protein-Associated Serine Proteases / Q000235:genetics*; D011110:Polymorphism, Genetic; D014376:Tuberculosis / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D037601:Mannose-Binding Lectin; C105296:MASP2 protein, human; D050606:Mannose-Binding Protein-Associated Serine Proteases