title: Incorportation of Ethyl Esters of EPA and DHA in Soybean Lecithin Using Rhizomucor miehei Lipase: Effect of Additives and Solvent-Free Conditions.
abstract: The transesterification of soybean lecithin with ethyl esters of polyunsaturated fatty acids (eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)) using immobilized lipase from Rhizomucor miehei was tested in the presence or absence of organic solvent (hexane) and additives (urea with Ca(2+) or Mg(2+)). The reaction was carried out at a water concentration of 4 % and ethyl ester to phospholipid mass ratio of 3:1. After 24 h of reaction without solvent or additive, fatty acid incorporation reached 29.1 % and thereafter increased only slightly. After 48 h, incorporation was highest in the presence of Mg(2+), urea, and solvent. After 72 h, it was highest with Mg(2+) and urea in the presence or absence of solvent (56.8 and 45.7 %, respectively). Incorporation of EPA and DHA was thus initially fast without solvent and additive, but was increased after prolonged reaction in the presence of Mg(2+) and urea with or without solvent. These results are innovative and promising since they show that immobilized Rhizomucor miehei lipase has potential as a biocatalyst for interesterification reactions without solvent.
authors.affiliation: Department of Biology, Chemistry and Geography, Université du Québec à Rimouski, Rimouski, Québec, Canada.
authors.forename: Nabil
authors.initials: N
authors.lastname: Marsaoui
authors.forename: Karim
authors.initials: K
authors.lastname: Naghmouchi
authors.forename: John
authors.initials: J
authors.lastname: Baah
authors.forename: Aly
authors.initials: A
authors.lastname: Raies
authors.forename: Serge
authors.initials: S
authors.lastname: Laplante
mesh_terms: D002118:Calcium / Q000494:pharmacology; D004281:Docosahexaenoic Acids / Q000737:chemistry*; D015118:Eicosapentaenoic Acid / Q000737:chemistry*; D004951:Esterification; D004952:Esters; D006586:Hexanes / Q000494:pharmacology; D054709:Lecithins / Q000494:pharmacology*; D008049:Lipase / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology; D020103:Rhizomucor / Q000201:enzymology*; D012997:Solvents / Q000494:pharmacology; D013025:Glycine max / Q000737:chemistry*; D014508:Urea / Q000494:pharmacology*; D014867:Water / Q000032:analysis
chemical_list: D004952:Esters; D006586:Hexanes; D054709:Lecithins; D012997:Solvents; D014867:Water; D004281:Docosahexaenoic Acids; D014508:Urea; D015118:Eicosapentaenoic Acid; D008049:Lipase; D008274:Magnesium; D002118:Calcium