title: Suppression of T-cell proliferation by and B7-H1 expression on human liver-derived stem cells.
abstract: BACKGROUND
  Many types of stem cells have been widely used for the treatment of liver diseases. The therapeutic effect of stem cells is predominantly based on the immune regulatory properties of these cells.
  
  
  METHODS
  We isolated human liver stem cells (HLSCs), which are considered intrahepatic stem cells, and examined their suppression of T-cell proliferation induced by phytohemagglutinin.
  
  
  RESULTS
  HLSCs inhibited phytohemagglutinin-induced T-cell proliferation not only in direct co-culture but also in indirect co-culture in a cell number-dependent manner. That is, T-cell proliferation was substantially inhibited by cell-to-cell contact regardless of soluble factor(s). B7-H1, a co-inhibitory molecule that relies on cell-to-cell contact, was found to be constitutively expressed at low levels on HLSCs. Furthermore, its expression was upregulated moderately by tumor necrosis factor-α and dramatically by interferon-γ.
  
  
  CONCLUSIONS
  These results suggest that HLSCs would have therapeutic effects through T-cell suppression in acute liver diseases.
authors.affiliation: Biomedical Research Institute, Lifeliver Co. Ltd., Yongin, Republic of Korea.
authors.forename: I K
authors.initials: IK
authors.lastname: Jang
authors.affiliation: Stem Cell and Regenerative Medicine Center, Research Institute for Future Medicine, Samsung Biomedical Research Institute, Samsung Medical Center, Seoul, Republic of Korea.
authors.forename: J H
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Dongguk University Research Institute of Biotechnology, Dongguk University, Seoul, Republic of Korea.
authors.forename: H H
authors.initials: HH
authors.lastname: Yoon
authors.affiliation: Stem Cell and Regenerative Medicine Center, Research Institute for Future Medicine, Samsung Biomedical Research Institute, Samsung Medical Center, Seoul, Republic of Korea.
authors.forename: H J
authors.initials: HJ
authors.lastname: Park
authors.affiliation: Stem Cell and Regenerative Medicine Center, Research Institute for Future Medicine, Samsung Biomedical Research Institute, Samsung Medical Center, Seoul, Republic of Korea.
authors.forename: Y A
authors.initials: YA
authors.lastname: Kim
authors.affiliation: Biomedical Research Institute, Lifeliver Co. Ltd., Yongin, Republic of Korea.
authors.forename: D H
authors.initials: DH
authors.lastname: Lee
authors.affiliation: Department of Surgery, Samsung Medical Center, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.
authors.forename: S H
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Surgery, Samsung Medical Center, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea. Electronic address: sklee3464@skku.edu.
authors.forename: S-K
authors.initials: SK
authors.lastname: Lee
mesh_terms: D053687:Adult Stem Cells / Q000276:immunology* / Q000378:metabolism; D060890:B7-H1 Antigen / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D006801:Humans; D008099:Liver / Q000166:cytology* / Q000276:immunology; D013601:T-Lymphocytes / Q000502:physiology*; D015854:Up-Regulation
chemical_list: D060890:B7-H1 Antigen; D015415:Biomarkers; C423236:CD274 protein, human