title: Characterization of a long-chain fatty acid-CoA ligase 1 gene and association between its SNPs and growth traits in the clam Meretrix meretrix.
abstract: Long-chain fatty acid-CoA ligases (ACSLs) play crucial roles in fatty acid (FA) metabolism. They convert free long-chain FA into acyl-CoAs, which are key intermediates in both anabolic and catabolic pathways. A long-chain fatty acid-CoA ligase gene was cloned in the clam Meretrix meretrix (MmeACSL1), with a full-length cDNA of 1865 bp encoding 475 amino acids. Its expression was only detected in hepatopancreas by semi-quantitative reverse transcription PCR. Expression level of MmeACSL1 exhibited a significant increase in a starvation experiment (P<0.05). This indicates that MmeACSL1 plays an important role in normal metabolism of M. meretrix and may be involved in energy supply and storage. Two exon SNPs and six intron SNPs were developed in this gene by direct sequencing. A marker-trait association analysis showed that five of these SNPs were significantly associated with growth traits (P<0.05). Also, haplotypes comprised of the five SNPs were revealed to be significantly growth-related (P<0.05), which further corroborates the relationship of this gene to growth traits of M. meretrix.
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Dai
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
authors.forename: Pin
authors.initials: P
authors.lastname: Huan
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Lu
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. Electronic address: bzliu@qdio.ac.cn.
authors.forename: Baozhong
authors.initials: B
authors.lastname: Liu
mesh_terms: D000818:Animals; D001483:Base Sequence; D049872:Bivalvia / Q000235:genetics* / Q000254:growth & development; D003066:Coenzyme A Ligases / Q000235:genetics*; D017931:DNA Primers; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D000098859:Long-Chain-Fatty-Acid-CoA Ligase
chemical_list: D017931:DNA Primers; D003066:Coenzyme A Ligases; D000098859:Long-Chain-Fatty-Acid-CoA Ligase
keywords: ACSL1; Growth; Meretrix meretrix; SNPs; Starvation