title: Interneurons and oligodendrocyte progenitors form a structured synaptic network in the developing neocortex.
abstract: NG2 cells, oligodendrocyte progenitors, receive a major synaptic input from interneurons in the developing neocortex. It is presumed that these precursors integrate cortical networks where they act as sensors of neuronal activity. We show that NG2 cells of the developing somatosensory cortex form a transient and structured synaptic network with interneurons that follows its own rules of connectivity. Fast-spiking interneurons, highly connected to NG2 cells, target proximal subcellular domains containing GABAA receptors with γ2 subunits. Conversely, non-fast-spiking interneurons, poorly connected with these progenitors, target distal sites lacking this subunit. In the network, interneuron-NG2 cell connectivity maps exhibit a local spatial arrangement reflecting innervation only by the nearest interneurons. This microcircuit architecture shows a connectivity peak at PN10, coinciding with a switch to massive oligodendrocyte differentiation. Hence, GABAergic innervation of NG2 cells is temporally and spatially regulated from the subcellular to the network level in coordination with the onset of oligodendrogenesis.
authors.affiliation: INSERM U1128, Paris, France.
authors.forename: David
authors.initials: D
authors.lastname: Orduz
authors.affiliation: INSERM U1128, Paris, France.
authors.forename: Paloma P
authors.initials: PP
authors.lastname: Maldonado
authors.affiliation: INSERM U1128, Paris, France.
authors.forename: Maddalena
authors.initials: M
authors.lastname: Balia
authors.affiliation: INSERM U1128, Paris, France.
authors.forename: Mateo
authors.initials: M
authors.lastname: Vélez-Fort
authors.affiliation: Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: de Sars
authors.affiliation: Department of Genetic and Behavioral Neuroscience, Gunma University Graduate School of Medicine, Maebashi, Japan.
authors.forename: Yuchio
authors.initials: Y
authors.lastname: Yanagawa
authors.affiliation: Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
authors.forename: Valentina
authors.initials: V
authors.lastname: Emiliani
authors.affiliation: INSERM U1128, Paris, France.
authors.forename: Maria Cecilia
authors.initials: MC
authors.lastname: Angulo
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation; D015870:Gene Expression; D017930:Genes, Reporter; D007395:Interneurons / Q000378:metabolism / Q000648:ultrastructure*; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D008867:Microtomy; D019579:Neocortex / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism; D058953:Neural Stem Cells / Q000378:metabolism / Q000648:ultrastructure*; D055495:Neurogenesis / Q000235:genetics; D009836:Oligodendroglia / Q000378:metabolism / Q000648:ultrastructure*; D018408:Patch-Clamp Techniques; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D011963:Receptors, GABA-A / Q000235:genetics / Q000378:metabolism; D013003:Somatosensory Cortex / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism; D013569:Synapses / Q000378:metabolism / Q000648:ultrastructure; D009435:Synaptic Transmission; D046509:Tissue Culture Techniques; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D008164:Luminescent Proteins; D021122:Protein Subunits; D011963:Receptors, GABA-A; C413663:fluorescent protein 583; C054562:yellow fluorescent protein, Bacteria; D005680:gamma-Aminobutyric Acid
keywords: GABAergic transmission; NG2 cells; cortical development; interneurons; mouse; neuroscience; paired-recordings; synapses