title: Mitochondria-targeted ratiometric fluorescent probe for real time monitoring of pH in living cells.
abstract: Pyridinium functioned 7-hydroxy coumarin was presented as the first mitochondria-targeted ratiometric fluorescent probe CP for real time monitoring pH in living cells. Compared with commercially available mitochondrial trackers, CP possesses high specificity to mitochondria in living cells as well as good biocompatibility. Meanwhile, CP displays excellent pH sensitivity and anti-interference capability. Confocal image experiments confirm that CP can monitor mitochondrial pH changes associated with the mitochondrial acidification, cellular apoptosis and stress response efficiently in real time.
authors.affiliation: Key Laboratory of Green Chemistry and Technology (Ministry of Education), College of Chemistry, Sichuan University, Chengdu 610064, PR China.
authors.forename: Ming-Yu
authors.initials: MY
authors.lastname: Wu
authors.affiliation: Key Laboratory of Green Chemistry and Technology (Ministry of Education), College of Chemistry, Sichuan University, Chengdu 610064, PR China; State Key Laboratory of Biotherapy/Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, PR China. Electronic address: kli@scu.edu.cn.
authors.forename: Kun
authors.initials: K
authors.lastname: Li
authors.affiliation: Key Laboratory of Green Chemistry and Technology (Ministry of Education), College of Chemistry, Sichuan University, Chengdu 610064, PR China.
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Liu
authors.affiliation: Key Laboratory of Green Chemistry and Technology (Ministry of Education), College of Chemistry, Sichuan University, Chengdu 610064, PR China.
authors.forename: Kang-Kang
authors.initials: KK
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Biotherapy/Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, PR China.
authors.forename: Yong-Mei
authors.initials: YM
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, PR China. Electronic address: zhouxd@scu.edu.cn.
authors.forename: Xue-Dong
authors.initials: XD
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Green Chemistry and Technology (Ministry of Education), College of Chemistry, Sichuan University, Chengdu 610064, PR China. Electronic address: xqyu@scu.edu.cn.
authors.forename: Xiao-Qi
authors.initials: XQ
authors.lastname: Yu
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008928:Mitochondria / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005456:Fluorescent Dyes
keywords: 7-Hydroxy coumarin; Mitochondria-targeted; Ratiometric; Real time; pH probe