title: Fascin Overexpression Promotes Cholangiocarcinoma RBE Cell Proliferation, Migration, and Invasion.
abstract: Fascin is overexpressed in various tumor tissues and is closely related to tumor metastasis and invasion. However, the role of fascin in cholangiocarcinoma RBE cells has not been clearly reported. This study aimed to establish a cholangiocarcinoma cell line with stable and high expression of fascin to observe the effect of fascin on cell proliferation, migration, and invasion. A fascin overexpression vector, pcDNA3.1-Fascin, was constructed and transfected into the human cholangiocarcinoma RBE cell line. The results of real-time polymerase chain reaction, Western blot, and immunofluorescence indicated that fascin was steadily and highly expressed in RBE cells. The results of 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide and colony formation assay indicated that upregulated fascin expression could enhance cholangiocarcinoma cell proliferation. The results of wound healing assay and transwell assay indicated that fascin could promote cholangiocarcinoma cell migration and invasion, and a further study found that the nuclear factor-κB signaling pathway was activated after upregulation of fascin, whereas E-cadherin expression in these cells was significantly decreased. Additionally, E-cadherin expression was significantly increased after inhibiting nuclear factor-κB activity using inhibitor or small interfering RNA, and E-cadherin expression was decreased by fascin overexpression after nuclear factor-κB inhibition, suggesting that nuclear factor-κB signaling pathway was not involved in the regulation of E-cadherin by fascin. In summary, the results of this study demonstrated that fascin effectively promoted cholangiocarcinoma RBE cell proliferation, migration, and invasion. This study provides evidence for fascin as a potential target in the treatment of cholangiocarcinoma.
authors.affiliation: Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang, China Department of General Surgery, The Fourth Affiliated Hospital of China Medical University, Shenyang, China.
authors.forename: Haiying
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang, China.
authors.forename: Fuquan
authors.initials: F
authors.lastname: Yang
authors.affiliation: Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang, China.
authors.forename: Wenyan
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang, China.
authors.forename: Chunjv
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang, China Department of General Surgery, The Fourth Affiliated Hospital of China Medical University, Shenyang, China liu_jingangyx@163.com.
authors.forename: Jingang
authors.initials: J
authors.lastname: Liu
mesh_terms: D015703:Antigens, CD; D001650:Bile Duct Neoplasms / Q000378:metabolism* / Q000473:pathology; D001653:Bile Ducts, Intrahepatic; D015820:Cadherins / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D018281:Cholangiocarcinoma / Q000378:metabolism* / Q000473:pathology; D015870:Gene Expression; D006801:Humans; D008840:Microfilament Proteins / Q000235:genetics* / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D009361:Neoplasm Invasiveness
chemical_list: D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; D002352:Carrier Proteins; C502658:FSCN1 protein, human; D008840:Microfilament Proteins; D016328:NF-kappa B
keywords: E-cadherin; cholangiocarcinoma; fascin; invasion; migration; proliferation