title: Wood chemical composition in species of Cactaceae: the relationship between lignification and stem morphology.
abstract: In Cactaceae, wood anatomy is related to stem morphology in terms of the conferred support. In species of cacti with dimorphic wood, a unique process occurs in which the cambium stops producing wide-band tracheids (WBTs) and produces fibers; this is associated with the aging of individuals and increases in size. Stem support and lignification have only been studied in fibrous tree-like species, and studies in species with WBTs or dimorphic wood are lacking. In this study, we approach this process with a chemical focus, emphasizing the role of wood lignification. We hypothesized that the degree of wood lignification in Cactaceae increases with height of the species and that its chemical composition varies with wood anatomy. To test this, we studied the chemical composition (cellulose, hemicellulose, and lignin content) in 13 species (2 WBTs wood, 3 dimorphic, and 8 fibrous) with contrasting growth forms. We also analyzed lignification in dimorphic and fibrous species to determine the chemical features of WBTs and fibers and their relationship with stem support. The lignin contents were characterized by Fourier transform infrared spectroscopy and high performance liquid chromatography. We found that 11 species have a higher percentage (>35%) of lignin in their wood than other angiosperms or gymnosperms. The lignin chemical composition in fibrous species is similar to that of other dicots, but it is markedly heterogeneous in non-fibrous species where WBTs are abundant. The lignification in WBTs is associated with the resistance to high water pressure within cells rather than the contribution to mechanical support. Dimorphic wood species are usually richer in syringyl lignin, and tree-like species with lignified rays have more guaiacyl lignin. The results suggest that wood anatomy and lignin distribution play an important role in the chemical composition of wood, and further research is needed at the cellular level.
authors.affiliation: Departamento de Botánica, Instituto de Biología, Universidad Nacional Autónoma de México, Mexico City, Mexico.
authors.forename: Jorge
authors.initials: J
authors.lastname: Reyes-Rivera
authors.affiliation: Unidad de Materiales, Centro de Investigación Científica de Yucatán, Mérida, Yucatán, Mexico.
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Canché-Escamilla
authors.affiliation: Programa de Botánica, Colegio de Postgraduados en Ciencias Agrícolas, Montecillo, Estado de México, Mexico.
authors.forename: Marcos
authors.initials: M
authors.lastname: Soto-Hernández
authors.affiliation: Departamento de Botánica, Instituto de Biología, Universidad Nacional Autónoma de México, Mexico City, Mexico.
authors.forename: Teresa
authors.initials: T
authors.lastname: Terrazas
mesh_terms: D029421:Cactaceae / Q000737:chemistry* / Q000145:classification / Q000254:growth & development; D002851:Chromatography, High Pressure Liquid; D008031:Lignin / Q000032:analysis*; D010802:Phylogeny; D018547:Plant Stems / Q000737:chemistry / Q000166:cytology / Q000648:ultrastructure; D013045:Species Specificity; D017550:Spectroscopy, Fourier Transform Infrared; D014934:Wood / Q000737:chemistry*
chemical_list: D008031:Lignin