title: DEPDC1 is a novel cell cycle related gene that regulates mitotic progression.
abstract: DEPDC1 is a recently identified novel tumor-related gene that is upregulated in several types of cancer and contributes to tumorigenesis. In this study, we have investigated the expression pattern and functional implications of DEPDC1 during cell cycle progression. Expression studies using synchronized cells demonstrated that DEPDC1 is highly expressed in the mitotic phase of the cell cycle. Immunofluorescence assays showed that DEPDC1 is predominantly localized in the nucleus during interphase and is redistributed into the whole cell upon nuclear membrane breakdown in metaphase. Subsequently, siRNA-mediated knockdown of DEPDC1 caused a significant mitotic arrest. Moreover, knockdown of DEPDC1 resulted in remarkable mitotic defects such as abnormal multiple nuclei and multipolar spindle structures accompanied by the upregulation of the A20 gene as well as several cell cycle-related genes such as CCNB1 and CCNB2. Taken together, our current observations strongly suggest that this novel cancerous gene, DEPDC1, plays a pivotal role in the regulation of proper mitotic progression.
authors.affiliation: Department of Otolaryngology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Mi
authors.affiliation: Department of Biochemistry and Molecular Biology, Chongqing Medical University; Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Chundong
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry and Molecular Biology, Chongqing Medical University; Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Youquan
authors.initials: Y
authors.lastname: Bu
authors.affiliation: Department of Biochemistry and Molecular Biology, Chongqing Medical University; Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Otolaryngology, The First Affiliated Hospital of Chongqing Medical University; Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Longxia
authors.initials: L
authors.lastname: He
authors.affiliation: Department of Otolaryngology, The First Affiliated Hospital of Chongqing Medical University; Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Biochemistry and Molecular Biology, Chongqing Medical University; Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Huifang
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Biochemistry and Molecular Biology, Chongqing Medical University; Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Biochemistry and Molecular Biology, Chongqing Medical University; Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Lei
authors.affiliation: Department of Otolaryngology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Zhu
mesh_terms: D059447:Cell Cycle Checkpoints / Q000235:genetics; D018385:Centrosome / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D020690:GTPase-Activating Proteins / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D006367:HeLa Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D008938:Mitosis / Q000235:genetics*; D009363:Neoplasm Proteins / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport; D034741:RNA, Small Interfering / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism; D000072598:Tumor Necrosis Factor alpha-Induced Protein 3; D015854:Up-Regulation / Q000235:genetics
chemical_list: C584411:DEPDC1 protein, human; D004268:DNA-Binding Proteins; D020690:GTPase-Activating Proteins; D047908:Intracellular Signaling Peptides and Proteins; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D034741:RNA, Small Interfering; C076529:TNFAIP3 protein, human; D000072598:Tumor Necrosis Factor alpha-Induced Protein 3