title: Transient receptor potential-canonical 3 modulates sperm motility and capacitation-associated protein tyrosine phosphorylation via [Ca2+]i mobilization.
abstract: Ca(2+) signaling is pivotal for sperm maturation, including the processes of motility, capacitation, and the acrosome reaction. As a Ca(2+) conductor, transient receptor potential-canonical 3 (TRPC3) plays an important role in somatic cells. However, the function of TRPC3 in sperm is not well understood. Here, a pharmacological approach was used to investigate the role and mechanism of TPRC3 in sperm function. The TRPC3 antagonist Pyr3 could inhibit sperm motility and accelerate capacitation-associated protein tyrosine phosphorylation in a time- and dose-dependent manner, regardless of the presence or absence of Ca(2+) in the incubation medium. Further investigation revealed that sperm [Ca(2+)]i fell immediately once Pyr3 was added to Ca(2+)-free medium, and then gradually increased and returned to baseline levels. Moreover, the [Ca(2+)]i levels markedly elevated when sperm were incubated for 30 min in the presence of Pyr3; this change was subsequently accompanied by a significant reduction in sperm mitochondrial membrane potential. This study suggested that TRPC3 can modulate sperm function via mobilization of sperm [Ca(2+)]i.
authors.affiliation: State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China University of Chinese Academy of Sciences, Beijing 100864, China.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Ru
authors.affiliation: State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China zhouych@sibcb.ac.cn ylzhang@sibcb.ac.cn.
authors.forename: Yuchuan
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China zhouych@sibcb.ac.cn ylzhang@sibcb.ac.cn.
authors.forename: Yonglian
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010766:Phosphorylation; D013075:Sperm Capacitation / Q000502:physiology*; D013081:Sperm Motility / Q000502:physiology*; D050052:TRPC Cation Channels / Q000037:antagonists & inhibitors / Q000502:physiology*; D014443:Tyrosine / Q000378:metabolism*
chemical_list: D050052:TRPC Cation Channels; C104603:TRPC3 cation channel; D014443:Tyrosine; D002118:Calcium
keywords: Pyr3; TRPC3; [Ca2+]i; capacitation; sperm motility