title: The Pathogenesis of Human Cervical Epithelium Cells Induced by Interacting with Trichomonas vaginalis.
abstract: BACKGROUND
  Trichomonas vaginalis is a protozoan parasite that occurs in the urogenital-vaginal tract and is the primary causative agent of trichomoniasis, a common sexually transmitted disease in humans. The aggregation of this protozoan tends to destroy epithelial cells and induce pathogenesis.
  
  
  RESULTS
  This study cultured T. vaginalis and human cervical epithelial cells (Z172) under the same conditions in the experiments. Following co-culturing for ten hours, the protozoans became attached to Z172, such that the cells presented a round shape and underwent shrinkage. Time-lapse recording and flow cytometry on interacted Z172 revealed that 70% had been disrupted, 18% presented a necrosis-like morphology and 8% showed signs of apoptosis. Gene expression profiling revealed in the seven inflammatory Z172 genes as well as in T. vaginalis genes that code for adhesion proteins 65 and 65-1.
  
  
  CONCLUSIONS
  These results suggest that cytopathogenic effects progress while Z172 is in contact with T. vaginalis, and the resulting morphological changes can be categorized as disruption.
authors.affiliation: Department of Parasitology, National Cheng Kung University, Tainan, Taiwan, Republic of China.
authors.forename: Wei-Chen
authors.initials: WC
authors.lastname: Lin
authors.affiliation: Department of Parasitology, National Cheng Kung University, Tainan, Taiwan, Republic of China.
authors.forename: Wei-Ting
authors.initials: WT
authors.lastname: Chang
authors.affiliation: Department of Parasitology, National Cheng Kung University, Tainan, Taiwan, Republic of China.
authors.forename: Tsuey-Yu
authors.initials: TY
authors.lastname: Chang
authors.affiliation: Department of Parasitology, National Cheng Kung University, Tainan, Taiwan, Republic of China.
authors.forename: Jyh-Wei
authors.initials: JW
authors.lastname: Shin
mesh_terms: D017209:Apoptosis / Q000235:genetics; D002448:Cell Adhesion; D002460:Cell Line; D002584:Cervix Uteri / Q000378:metabolism / Q000469:parasitology / Q000473:pathology; D018920:Coculture Techniques; D004847:Epithelial Cells / Q000378:metabolism / Q000469:parasitology / Q000473:pathology*; D005260:Female; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006790:Host-Parasite Interactions; D006801:Humans; D015800:Protozoan Proteins / Q000235:genetics* / Q000378:metabolism; D059008:Time-Lapse Imaging; D014246:Trichomonas vaginalis / Q000235:genetics / Q000472:pathogenicity
chemical_list: D015800:Protozoan Proteins