title: Amphipathic DNA origami nanoparticles to scaffold and deform lipid membrane vesicles.
abstract: We report a synthetic biology-inspired approach for the engineering of amphipathic DNA origami structures as membrane-scaffolding tools. The structures have a flat membrane-binding interface decorated with cholesterol-derived anchors. Sticky oligonucleotide overhangs on their side facets enable lateral interactions leading to the formation of ordered arrays on the membrane. Such a tight and regular arrangement makes our DNA origami capable of deforming free-standing lipid membranes, mimicking the biological activity of coat-forming proteins, for example, from the I-/F-BAR family.
authors.affiliation: Biotechnology Center of the TU Dresden, Tatzberg 47/51, 01307 Dresden (Germany).|Department of Cytobiochemistry, Faculty of Biotechnology, University of Wrocław ul. F. Joliot-Curie 14a, 50383 Wrocław (Poland).
authors.forename: Aleksander
authors.initials: A
authors.lastname: Czogalla
authors.affiliation: Institute for Molecular Cell Biology, University of Münster, Schlossplatz 5, 48149 Münster (Germany).
authors.forename: Dominik J
authors.initials: DJ
authors.lastname: Kauert
authors.affiliation: Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried (Germany) http://www.biochem.mpg.de/en/rd/schwille/
authors.forename: Henri G
authors.initials: HG
authors.lastname: Franquelim
authors.affiliation: B CUBE-Center for Molecular Bioengineering, Dresden, University of Technology, Arnoldstraße 18, 01307 Dresden (Germany).
authors.forename: Veselina
authors.initials: V
authors.lastname: Uzunova
authors.affiliation: B CUBE-Center for Molecular Bioengineering, Dresden, University of Technology, Arnoldstraße 18, 01307 Dresden (Germany).
authors.forename: Yixin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institute for Molecular Cell Biology, University of Münster, Schlossplatz 5, 48149 Münster (Germany).
authors.forename: Ralf
authors.initials: R
authors.lastname: Seidel
authors.affiliation: Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried (Germany) http://www.biochem.mpg.de/en/rd/schwille/. schwille@biochem.mpg.de.
authors.forename: Petra
authors.initials: P
authors.lastname: Schwille
mesh_terms: D002784:Cholesterol / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D004058:Diffusion; D005456:Fluorescent Dyes / Q000737:chemistry; D008563:Membrane Lipids / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009841:Oligonucleotides / Q000737:chemistry; D053835:Unilamellar Liposomes / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D008563:Membrane Lipids; D009841:Oligonucleotides; D053835:Unilamellar Liposomes; D004247:DNA; D002784:Cholesterol
keywords: diffusion; lipid membranes; membrane deformation; oligomerization; origami DNA structures