title: The expression of CDK1 is associated with proliferation and can be a prognostic factor in epithelial ovarian cancer.
abstract: Overexpression of cyclin-dependent kinase 1 (CDK1) has been noted to correlation with several human cancers. However, the effects of CDK1 on ovarian cancer development remain unclear. The aim of this study was to examine the effect of CDK1 and related mechanism in the proliferation and resistance to chemotherapeutic drugs of epithelial ovarian cancer (EOC). Immunohistochemical analysis was performed in 119 human ovarian cancer samples, and the data were correlated with clinicopathologic features. Furthermore, Western blot analysis was performed for CDK1 in EOC samples and cell lines to evaluate their protein levels and molecular interaction. Kaplan-Meier survival analysis showed that strong expression of CDK1 exhibited a significant correlation with poor prognosis in human EOC (P = 0.02). Meanwhile, we found that knockdown CDK1 by shCDK1 promoted the apoptosis rate and increased the sensitivity to chemotherapy drugs. Thus, CDK1 might serve as a prognostic marker, and it might be of great value for experimental therapies in EOC.
authors.affiliation: Department of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Jiangsu Province Key Laboratory for Information and Molecular Drug Target, Nantong University, Nantong, 226001, Jiangsu Province, China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Xi
authors.forename: Menghui
authors.initials: M
authors.lastname: Huang
authors.forename: Yingying
authors.initials: Y
authors.lastname: Wang
authors.forename: Jianxin
authors.initials: J
authors.lastname: Zhong
authors.forename: Rong
authors.initials: R
authors.lastname: Liu
authors.forename: Guiqin
authors.initials: G
authors.lastname: Xu
authors.forename: Lifei
authors.initials: L
authors.lastname: Jiang
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Fang
authors.forename: Shuyun
authors.initials: S
authors.lastname: Yang
mesh_terms: D000328:Adult; D000368:Aged; D016203:CDC2 Protein Kinase; D000077216:Carcinoma, Ovarian Epithelial; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival; D002945:Cisplatin / Q000008:administration & dosage; D018844:Cyclin-Dependent Kinases / Q000096:biosynthesis* / Q000235:genetics; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008875:Middle Aged; D009367:Neoplasm Staging; D009375:Neoplasms, Glandular and Epithelial / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology; D010051:Ovarian Neoplasms / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology; D011379:Prognosis
chemical_list: D016203:CDC2 Protein Kinase; C528899:CDK1 protein, human; D018844:Cyclin-Dependent Kinases; D002945:Cisplatin