title: [Eficient inhibition of human influenza A virus by oligonucleotides electrostatically fixed on polylysine-containing TiO2 nanoparticles].
abstract: Antiviral activity of TiO2 * PL * DNA nanobiocomposites was studied on the MDCK cell culture infected with influenza A virus (subtype H3N2). DNA fragments in the nanocomposites are electrostatically bound to titanium dioxide nanoparticles pre-covered with polylysine. It was shown that TiO2 * PL * DNA(v3') nanocomposite bearing the DNA(v3') fragment targeted to the 3'-end of the noncoding region of segment 5 of viral RNA specifically inhibited the virus reproduction with the efficiency of 99.8 and 99.9% (or by factors of~400 and 1000) at a low concentration of DNA(v3') in nanocomposite (0.1 and 0.2 µM, respectively). The TiO2 * PL * DNA(r) nanocomposite containing oligonucleotide noncomplementary to viral RNA or the oligonucleotide unbound to the nanoparticles show very low antiviral activity (inhibition by factors of~3.5 and 1.3, respectively).
authors.forename: A S
authors.initials: AS
authors.lastname: Levina
authors.forename: M N
authors.initials: MN
authors.lastname: Repkova
authors.forename: Z R
authors.initials: ZR
authors.lastname: Ismagilov
authors.forename: N V
authors.initials: NV
authors.lastname: Shikina
authors.forename: N A
authors.initials: NA
authors.lastname: Mazurkova
authors.forename: V F
authors.initials: VF
authors.lastname: Zarytova
mesh_terms: D000998:Antiviral Agents; D002460:Cell Line; D006801:Humans; D053122:Influenza A Virus, H3N2 Subtype / Q000187:drug effects* / Q000472:pathogenicity; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D009841:Oligonucleotides / Q000008:administration & dosage* / Q000737:chemistry; D011107:Polylysine / Q000737:chemistry; D012367:RNA, Viral / Q000187:drug effects; D055672:Static Electricity; D014025:Titanium / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D000998:Antiviral Agents; D009841:Oligonucleotides; D012367:RNA, Viral; C009495:titanium dioxide; D011107:Polylysine; D014025:Titanium