title: Isolation and identification of bacteria by means of Raman spectroscopy.
abstract: Bacterial detection is a highly topical research area, because various fields of application will benefit from the progress being made. Consequently, new and innovative strategies which enable the investigation of complex samples, like body fluids or food stuff, and improvements regarding the limit of detection are of general interest. Within this review the prospects of Raman spectroscopy as a reliable tool for identifying bacteria in complex samples are discussed. The main emphasis of this work is on important aspects of applying Raman spectroscopy for the detection of bacteria like sample preparation and the identification process. Several approaches for a Raman compatible isolation of bacterial cells have been developed and applied to different matrices. Here, an overview of the limitations and possibilities of these methods is provided. Furthermore, the utilization of Raman spectroscopy for diagnostic purposes, food safety and environmental issues is discussed under a critical view.
authors.affiliation: Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Germany; InfectoGnostics Research Campus Jena, Center for Applied Research, Philosophenweg 7, 07743 Jena, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: Pahlow
authors.affiliation: Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Germany; InfectoGnostics Research Campus Jena, Center for Applied Research, Philosophenweg 7, 07743 Jena, Germany.
authors.forename: Susann
authors.initials: S
authors.lastname: Meisel
authors.affiliation: Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Germany; Leibniz Institute of Photonic Technology, Albert-Einstein-Straße 9, 07745 Jena, Germany; InfectoGnostics Research Campus Jena, Center for Applied Research, Philosophenweg 7, 07743 Jena, Germany.
authors.forename: Dana
authors.initials: D
authors.lastname: Cialla-May
authors.affiliation: Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Germany; Leibniz Institute of Photonic Technology, Albert-Einstein-Straße 9, 07745 Jena, Germany; InfectoGnostics Research Campus Jena, Center for Applied Research, Philosophenweg 7, 07743 Jena, Germany.
authors.forename: Karina
authors.initials: K
authors.lastname: Weber
authors.affiliation: Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Germany; InfectoGnostics Research Campus Jena, Center for Applied Research, Philosophenweg 7, 07743 Jena, Germany.
authors.forename: Petra
authors.initials: P
authors.lastname: Rösch
authors.affiliation: Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Germany; Leibniz Institute of Photonic Technology, Albert-Einstein-Straße 9, 07745 Jena, Germany; InfectoGnostics Research Campus Jena, Center for Applied Research, Philosophenweg 7, 07743 Jena, Germany. Electronic address: juergen.popp@ipht-jena.de.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Popp
mesh_terms: D000818:Animals; D001419:Bacteria / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods; D059022:Food Safety / Q000379:methods; D006801:Humans; D013059:Spectrum Analysis, Raman / Q000379:methods*
keywords: Classification; Complex matrices; Extraction; Pathogen detection; Raman microspectroscopy; Sample preparation; Surface enhanced Raman scattering; Validation