title: miR-203 downregulates Yes-1 and suppresses oncogenic activity in human oral cancer cells.
abstract: The purpose of this study was to elucidate the molecular mechanisms of microRNA-203 (miR-203) as a tumor suppressor in KB human oral cancer cells. MicroRNA microarray results showed that the expression of miR-203 was significantly down-regulated in KB cells compared with normal human oral keratinocytes. The viability of KB cells was decreased by miR-203 in the time- and dose-dependent manners. In addition, over-expressed miR-203 not only increased the nuclear condensation but also significantly increased the apoptotic population of KB cells. These results indicated that the over-expression of miR-203 induced apoptosis of KB cells. Furthermore, the target gene array analyses revealed that the expression of Yes-1, a member of the Src family kinases (SFKs), was significantly down-regulated by miR-203 in KB cells. Moreover, both the mRNA and protein levels of Yes-1 were strongly reduced in KB cells transfected with miR-203. Therefore, these results indicated that Yes-1 is predicted to be a potential target gene of miR-203. Through a luciferase activity assay, miR-203 was confirmed to directly targets the Yes-1 3' untranslated region (UTR) to suppress gene expression. Therefore, our findings indicate that miR-203 induces the apoptosis of KB cells by directly targeting Yes-1, suggesting its application in anti-cancer therapeutics.
authors.affiliation: Oral Biology Research Institute, Chosun University School of Dentistry, 375 Seosuk-Dong, Dong-Gu, Gwangju 501-759, Republic of Korea.
authors.forename: Seul-Ah
authors.initials: SA
authors.lastname: Lee
authors.affiliation: Oral Biology Research Institute, Chosun University School of Dentistry, 375 Seosuk-Dong, Dong-Gu, Gwangju 501-759, Republic of Korea.
authors.forename: Jae-Sung
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Oral Biology Research Institute, Chosun University School of Dentistry, 375 Seosuk-Dong, Dong-Gu, Gwangju 501-759, Republic of Korea.
authors.forename: Sun-Young
authors.initials: SY
authors.lastname: Park
authors.affiliation: Oral Biology Research Institute, Chosun University School of Dentistry, 375 Seosuk-Dong, Dong-Gu, Gwangju 501-759, Republic of Korea.
authors.forename: Heung-Joong
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Oral Biology Research Institute, Chosun University School of Dentistry, 375 Seosuk-Dong, Dong-Gu, Gwangju 501-759, Republic of Korea.
authors.forename: Sun-Kyoung
authors.initials: SK
authors.lastname: Yu
authors.affiliation: Oral Biology Research Institute, Chosun University School of Dentistry, 375 Seosuk-Dong, Dong-Gu, Gwangju 501-759, Republic of Korea.
authors.forename: Chun Sung
authors.initials: CS
authors.lastname: Kim
authors.affiliation: Department of Biomedical Science, Chosun University, 375 Seosuk-Dong, Dong-Gu, Gwangju 501-759, Republic of Korea.
authors.forename: Hong Sung
authors.initials: HS
authors.lastname: Chun
authors.affiliation: Department of Dental Hygiene, Gwangju Health University, 683-3 Sinchang-Dong, Gwangsan-Gu, Gwangju 506-701, Republic of Korea.
authors.forename: Jeongsun
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Oral Anatomy, Dankook University College of Dentistry, Cheonan 330-714, Republic of Korea.
authors.forename: Jong-Tae
authors.initials: JT
authors.lastname: Park
authors.affiliation: Oral Biology Research Institute, Chosun University School of Dentistry, 375 Seosuk-Dong, Dong-Gu, Gwangju 501-759, Republic of Korea.
authors.forename: Daesan
authors.initials: D
authors.lastname: Go
authors.affiliation: Oral Biology Research Institute, Chosun University School of Dentistry, 375 Seosuk-Dong, Dong-Gu, Gwangju 501-759, Republic of Korea. Electronic address: kdk@chosun.ac.kr.
authors.forename: Do Kyung
authors.initials: DK
authors.lastname: Kim
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D017209:Apoptosis / Q000235:genetics*; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D016147:Genes, Tumor Suppressor; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000627:therapeutic use; D009062:Mouth Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D020411:Oligonucleotide Array Sequence Analysis; D051575:Proto-Oncogene Proteins c-yes / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection
chemical_list: D020413:3' Untranslated Regions; C546703:MIRN203 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D051575:Proto-Oncogene Proteins c-yes; C489834:YES1 protein, human
keywords: Apoptosis; Oral cancer cells; Tumor suppressor; Yes-1; miR-203