title: Influence of static and alternating magnetic fields on U937 cell viability.
abstract: Presented studies were conducted to assess the influence of alternating (AC) and/or static (DC) magnetic fields (MF) or combined with puromycin (PMC) on U937 cell line viability. The magnitude of DC MF was enclosed in the range (2 ÷ 6) mT. In case of AC MF, four frequencies were set: 12 Hz, 25 Hz, 35 Hz, 50 Hz and magnetic induction value was adjusted to 6.5(rms) mT. The reaction of samples exposed to MF and/or PMC was presented as cell viability coefficient S defined as a ratio of viable cells in the sample to viable cells in the relevant control sample, analyzed by flow cytometry. For PMC treated sample the percentage of viable cells decreased about factor 3. MF alone did not influence cell viability regardless of the type of exposure while simultaneous action of ACDC MF mode and PMC significantly influenced viability of U937 cells. The viability coefficient S was in the range of (0.13 ∓ 0.07 ÷ 0.64 ∓ 0.20) and exerted a non-linear relation with frequency of the AC MF component. The maximal lethal influence (S = 0.13 ∓ 0.07) was observed for DC MF = 6 mT and frequency of AC MF equal to 35 Hz. Observed bio-effects were confronted with the physical models assuming the Ca2+ ions transport disturbance and/or protein complexes dysfunction in cells due to MF action.
authors.affiliation: Department of Biophysics, Jagiellonian University Medical College, Krakow, Poland. km.wojcik@uj.edu.pl.
authors.forename: Karolina
authors.initials: K
authors.lastname: Wócik-Piotrowicz
authors.forename: Jolanta
authors.initials: J
authors.lastname: Kaszuba-Zwoińska
authors.forename: Eugeniusz
authors.initials: E
authors.lastname: Rokita
authors.forename: Piotr
authors.initials: P
authors.lastname: Thor
authors.forename: Paulina
authors.initials: P
authors.lastname: Chrobik
authors.forename: Zenon
authors.initials: Z
authors.lastname: Nieckarz
authors.forename: Józef
authors.initials: J
authors.lastname: Michalski
mesh_terms: D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology*; D017209:Apoptosis / Q000528:radiation effects; D049109:Cell Proliferation / Q000187:drug effects* / Q000528:radiation effects*; D004574:Electromagnetic Fields; D006801:Humans; D008954:Models, Biological; D011691:Puromycin / Q000494:pharmacology*; D020298:U937 Cells
chemical_list: D000964:Antimetabolites, Antineoplastic; D011691:Puromycin
keywords: Magnetic Field; Puromycin; U937 Cell Line; Viable Cells