title: [Inhibitory effects of ethyl acetate extract of Huanglian Jiedu decoction on hyphae development of Candida albicans].
abstract: OBJECTIVE
  To investigate the effects of ethyl acetate extract of Huanglian Jiedu decoction (EAHD) on hyphae development of Candida albicans.
  
  
  METHODS
  Inverted microscope, fluorescence microscope, SEM were applied to inspect the Morphological change of C. albicans treated by EAHD at different concentrations. Solid agar plate was utilized to evaluate the colony morphology. Quantitative Real-ime PCR(qRT-PCR) was adopted to observe the expression of hyphae-specific genes such as HWP1, ALS3, UME6, CSH1, SUN41, CaPDE2.
  
  
  RESULTS
  EAHD with concentration of 312 and 1 250 mg . L-1 could inhibit formation of hyphae and colony morphology. The expression of HWP1, ALS3, UME6, CSH1 were downregulated 4. 13, 3. 64, 2. 46, 2. 75 folds ,while the expression of SUN41 were upregulated 7. 26 folds, CaPDE2 keep unchanged.
  
  
  CONCLUSIONS
  EAHD could inhibit formation of hyphae and colony morphologies of C. albicans through downregulating HWP1, ALS3, UME6 and CSH1.
authors.forename: Tian-ming
authors.initials: TM
authors.lastname: Wang
authors.forename: Yuan-yuan
authors.initials: YY
authors.lastname: Yan
authors.forename: Gao-xiang
authors.initials: GX
authors.lastname: Shi
authors.forename: Dan
authors.initials: D
authors.lastname: Xia
authors.forename: Jing
authors.initials: J
authors.lastname: Shao
authors.forename: Chang-zhong
authors.initials: CZ
authors.lastname: Wang
mesh_terms: D000085:Acetates; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development; D002176:Candida albicans / Q000166:cytology / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D015536:Down-Regulation; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005656:Fungal Proteins / Q000235:genetics; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D025301:Hyphae; D008516:Medicine, Chinese Traditional; D008856:Microscopy, Fluorescence; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000085:Acetates; D004365:Drugs, Chinese Herbal; D005656:Fungal Proteins; C494417:huanglian; C007650:ethyl acetate