title: Isolation of extracellular vesicles: Determining the correct approach (Review).
abstract: The discovery of extracellular vesicles (EVs) has revised the interpretation of intercellular communication. It is now well established that EVs play a significant role in coagulation, inflammation, cancer and stem cell renewal and expansion. Their release presents an intriguing, transporting/trafficking network of biologically active molecules, which are able to reach and modulate the function/behavior of the target cells in a variety of ways. Moreover, the presence of EVs in various body fluids points to their potential for use as biomarkers and prognostic indicators in the surveillance/monitoring of a variety of diseases. Although vast knowledge on the subject of EVs has accumulated over the years, there are still fundamental issues associated with the correct approach for their isolation. This review comprises the knowledge on EV isolation techniques that are currently available. The aim of this reveiw was to make both experienced researchers and newcomers to the field aware that different types of EVs require unique isolation approaches. The realization of this 'uniqueness' is the first step in the right direction for the complete assessment of EVs.
authors.affiliation: Department of Clinical Immunology and Transplantology, Jagiellonian University Medical College, 30-663 Krakow, Poland.
authors.forename: Rafal
authors.initials: R
authors.lastname: Szatanek
authors.affiliation: Department of Clinical Immunology and Transplantology, Jagiellonian University Medical College, 30-663 Krakow, Poland.
authors.forename: Jarek
authors.initials: J
authors.lastname: Baran
authors.affiliation: Department of Clinical Immunology and Transplantology, Jagiellonian University Medical College, 30-663 Krakow, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Siedlar
authors.affiliation: Department of Clinical Immunology and Transplantology, Jagiellonian University Medical College, 30-663 Krakow, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Baj-Krzyworzeka
mesh_terms: D000327:Adsorption; D001692:Biological Transport; D001826:Body Fluids / Q000166:cytology; D002450:Cell Communication; D018929:Cell Culture Techniques / Q000379:methods*; D055252:Cell-Derived Microparticles / Q000502:physiology*; D002850:Chromatography, Gel; D017077:Culture Media, Conditioned; D055354:Exosomes / Q000502:physiology*; D006801:Humans; D014461:Ultracentrifugation
chemical_list: D017077:Culture Media, Conditioned