title: [Evaluation of mass spectrometry: MALDI-TOF MS for fast and reliable yeast identification].
abstract: The matrix-assisted laser desorption/ionization time-of-flight mass spectrometry technique known as MALDI-TOF MS is a tool used for the identification of clinical pathogens by generating a protein spectrum that is unique for a given species. In this study we assessed the identification of clinical yeast isolates by MALDI-TOF MS in a university hospital from Argentina and compared two procedures for protein extraction: a rapid method and a procedure based on the manufacturer's recommendations. A short protein extraction procedure was applied in 100 isolates and the rate of correct identification at genus and species level was 98.0%. In addition, we analyzed 201 isolates, previously identified by conventional methods, using the methodology recommended by the manufacturer and there was 95.38% coincidence in the identification at species level. MALDI TOF MS showed to be a fast, simple and reliable tool for yeast identification.
authors.affiliation: Laboratorio de Bacteriología, Micología y Parasitología, Centro de Educación Médica e Investigaciones Clínicas Norberto Quirno (CEMIC), Ciudad Autónoma de Buenos Aires (CABA), Argentina. Electronic address: srelloso@yahoo.com.
authors.forename: María S
authors.initials: MS
authors.lastname: Relloso
authors.affiliation: Laboratorio de Bacteriología, Micología y Parasitología, Centro de Educación Médica e Investigaciones Clínicas Norberto Quirno (CEMIC), Ciudad Autónoma de Buenos Aires (CABA), Argentina.
authors.forename: Jimena
authors.initials: J
authors.lastname: Nievas
authors.affiliation: Laboratorio de Bacteriología, Micología y Parasitología, Centro de Educación Médica e Investigaciones Clínicas Norberto Quirno (CEMIC), Ciudad Autónoma de Buenos Aires (CABA), Argentina.
authors.forename: Santiago
authors.initials: S
authors.lastname: Fares Taie
authors.affiliation: Laboratorio de Bacteriología, Micología y Parasitología, Centro de Educación Médica e Investigaciones Clínicas Norberto Quirno (CEMIC), Ciudad Autónoma de Buenos Aires (CABA), Argentina.
authors.forename: Victoria
authors.initials: V
authors.lastname: Farquharson
authors.affiliation: Instituto de Microbiología y Parasitología Médica, Universidad de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas (IMPaM, UBA CONICET), Ciudad Autónoma de Buenos Aires (CABA), Argentina.
authors.forename: María T
authors.initials: MT
authors.lastname: Mujica
authors.affiliation: Laboratorio de Bacteriología, Micología y Parasitología, Centro de Educación Médica e Investigaciones Clínicas Norberto Quirno (CEMIC), Ciudad Autónoma de Buenos Aires (CABA), Argentina.
authors.forename: Vanesa
authors.initials: V
authors.lastname: Romano
authors.affiliation: Laboratorio de Bacteriología, Micología y Parasitología, Centro de Educación Médica e Investigaciones Clínicas Norberto Quirno (CEMIC), Ciudad Autónoma de Buenos Aires (CABA), Argentina.
authors.forename: Mariela S
authors.initials: MS
authors.lastname: Zarate
authors.affiliation: Laboratorio de Bacteriología, Micología y Parasitología, Centro de Educación Médica e Investigaciones Clínicas Norberto Quirno (CEMIC), Ciudad Autónoma de Buenos Aires (CABA), Argentina.
authors.forename: Jorgelina
authors.initials: J
authors.lastname: Smayevsky
mesh_terms: D002175:Candida / Q000145:classification / Q000302:isolation & purification; D005656:Fungal Proteins / Q000032:analysis; D016533:Mycological Typing Techniques / Q000379:methods*; D015203:Reproducibility of Results; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D015003:Yeasts / Q000145:classification* / Q000302:isolation & purification
chemical_list: D005656:Fungal Proteins
keywords: Identificación; Identification; Levaduras; MALDI-TOF MS; Yeast