title: Kir3 channels undergo arrestin-dependant internalization following delta opioid receptor activation.
abstract: Kir3 channels control excitability in the nervous system and the heart. Their surface expression is strictly regulated, but mechanisms responsible for channel removal from the membrane remain incompletely understood. Using transfected cells, we show that Kir3.1/3.2 channels and delta opioid receptors (DORs) associate in a complex which persists during receptor activation, behaving as a scaffold that allows beta-arrestin (βarr) to interact with both signaling partners. This organization favored co-internalization of DORs and Kir3 channels in a βarr-dependent manner via a clathrin/dynamin-mediated endocytic path. Taken together, these findings identify a new way of modulating Kir3 channel availability at the membrane and assign a putatively novel role for βarrs in regulating canonical effectors for G protein-coupled receptors.
authors.affiliation: Sainte-Justine Hospital Research Center, 3175 Cote Ste-Catherine, Montreal, QC, H3T 1C5, Canada.
authors.forename: Karim
authors.initials: K
authors.lastname: Nagi
authors.forename: Iness
authors.initials: I
authors.lastname: Charfi
authors.forename: Graciela
authors.initials: G
authors.lastname: Pineyro
mesh_terms: D000818:Animals; D019390:Arrestins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D002966:Clathrin / Q000378:metabolism; D034281:Dynamins / Q000378:metabolism; D004705:Endocytosis / Q000502:physiology; D051676:G Protein-Coupled Inwardly-Rectifying Potassium Channels / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D051381:Rats; D017465:Receptors, Opioid, delta / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D000071557:beta-Arrestins
chemical_list: D019390:Arrestins; D002966:Clathrin; D051676:G Protein-Coupled Inwardly-Rectifying Potassium Channels; D017465:Receptors, Opioid, delta; D000071557:beta-Arrestins; D034281:Dynamins