title: Clear distinction between Burkholderia mallei and Burkholderia pseudomallei using fluorescent motB primers.
abstract: BACKGROUND
  A frame-shift mutation in the flagellum motor gene motB coding for the chemotaxis MotB protein of Burkholderia mallei has been utilized to design a conventional duplex PCR assay with fluorescent labelled primers.
  
  
  RESULTS
  Species specificity was tested with a panel of 13 Burkholderia type strains. A total of 41 B. mallei field strains, 36 B. pseudomallei field strains, and 1 B. thailandensis field strain from different geographic regions were tested and correctly identified. Testing of 55 non-Burkholderia bacterial species revealed 100% specificity of the assay. The minimum detection limit was 1 pg DNA or 160 GE for B. mallei and 130 GE for B. pseudomallei, respectively.
  
  
  CONCLUSIONS
  This assay enables the clear distinction between B. mallei and B. pseudomallei/B. thailandensis.
authors.affiliation: Friedrich-Loeffler-Institut, Bundesforschungsinstitut für Tiergesundheit, Naumburger Str. 96a, DE-07743, Jena, Germany. gernot.schmoock@fli.bund.de.
authors.forename: Gernot
authors.initials: G
authors.lastname: Schmoock
authors.affiliation: Friedrich-Loeffler-Institut, Bundesforschungsinstitut für Tiergesundheit, Naumburger Str. 96a, DE-07743, Jena, Germany. mandy.elschner@fli.bund.de.|National and OIE Reference Laboratory for Glanders, Friedrich-Loeffler-Institut, Bundesforschungsinstitut für Tiergesundheit, Naumburger Str. 96a, DE-07743, Jena, Germany. mandy.elschner@fli.bund.de.
authors.forename: Mandy
authors.initials: M
authors.lastname: Elschner
authors.affiliation: Friedrich-Loeffler-Institut, Bundesforschungsinstitut für Tiergesundheit, Naumburger Str. 96a, DE-07743, Jena, Germany. lisa.sprague@fli.bund.de.
authors.forename: Lisa D
authors.initials: LD
authors.lastname: Sprague
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D042726:Burkholderia mallei / Q000145:classification* / Q000235:genetics; D016957:Burkholderia pseudomallei / Q000145:classification* / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; C064779:MotB protein, Bacteria