title: Effect of FosPeg® mediated photoactivation on P-gp/ABCB1 protein expression in human nasopharyngeal carcinoma cells.
abstract: Multidrug resistance (MDR) refers to the ability of cancer cells to develop cross resistance to a range of anticancer drugs which are structurally and functionally unrelated. P-glycoprotein (P-gp) is the best studied MDR phenotype in photodynamic therapy (PDT) treated cells. Our pervious study demonstrated that FosPeg® mediated PDT is effective to NPC cell line models. In this in vitro study, the expression of MDR1 gene and its product P-gp in undifferentiated, poorly differentiated and well differentiated human nasopharyngeal carcinoma (NPC) cells were investigated. The influence of P-gp efflux activities on photosensitizer FosPeg® was also examined. Regardless of the differentiation status, PDT tested NPC cell lines all expressed P-gp protein. Results indicated that FosPeg® photoactivation could heighten the expression of MDR1 gene and P-gp transporter protein in a dose dependent manner. Up to 2-fold increase of P-gp protein expression were seen in NPC cells after FosPeg® mediated PDT. Interestingly, our finding demonstrated that FosPeg® mediated PDT efficiency is independent to the MDR1 gene and P-gp protein expression in NPC cells. FosPeg® itself is not the substrate of P-gp transporter protein and no efflux of FosPeg® were observed in NPC cells. Therefore, the PDT efficiency would not be affected even though FosPeg® mediated PDT could induce MDR1 gene and P-gp protein expression in NPC cells. FosPeg® mediated PDT could be a potential therapeutic approach for MDR cancer patients.
authors.affiliation: Medical Laboratory Science, Department of Health Technology & Informatics, Hong Kong Polytechnic University, Hong Kong Special Administrative Region; Department of Medical Science, Tung Wah College, Hong Kong Special Administrative Region. Electronic address: rickywu@twc.edu.hk.
authors.forename: R W K
authors.initials: RWK
authors.lastname: Wu
authors.affiliation: Department of Medical Science, Tung Wah College, Hong Kong Special Administrative Region.
authors.forename: E S M
authors.initials: ESM
authors.lastname: Chu
authors.affiliation: College of Photonic & Electronic Engineering, Fujian Normal University, Fuzhou, PR China; University of Colorado Denver Cancer Center, CO, USA.
authors.forename: Z
authors.initials: Z
authors.lastname: Huang
authors.affiliation: School of Chinese Medicine, Chinese University of Hong Kong, Hong Kong Special Administrative Region.
authors.forename: C S
authors.initials: CS
authors.lastname: Xu
authors.affiliation: Medical Laboratory Science, Department of Health Technology & Informatics, Hong Kong Polytechnic University, Hong Kong Special Administrative Region.
authors.forename: C W
authors.initials: CW
authors.lastname: Ip
authors.affiliation: Medical Laboratory Science, Department of Health Technology & Informatics, Hong Kong Polytechnic University, Hong Kong Special Administrative Region. Electronic address: christine.yow@polyu.edu.hk.
authors.forename: C M N
authors.initials: CMN
authors.lastname: Yow
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000235:genetics / Q000378:metabolism; D002277:Carcinoma; D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000528:radiation effects; D006801:Humans; D008027:Light; D008081:Liposomes / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D008652:Mesoporphyrins / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; D008081:Liposomes; D008652:Mesoporphyrins; D017319:Photosensitizing Agents; D012333:RNA, Messenger; C072269:temoporfin