title: Effect of plasmid replication deregulation via inc mutations on E. coli proteome & simple flux model analysis.
abstract: When the replication of a plasmid based on sucrose selection is deregulated via the inc1 and inc2 mutations, high copy numbers (7,000 or greater) are attained while the growth rate on minimal medium is negligibly affected. Adaptions were assumed to be required in order to sustain the growth rate. Proteomics indicated that indeed a number of adaptations occurred that included increased expression of ribosomal proteins and 2-oxoglutarate dehydrogenase. The operating space prescribed by a basic flux model that maintained phenotypic traits (e.g. growth, byproducts, etc.) within typical bounds of resolution was consistent with the flux implications of the proteomic changes.
authors.affiliation: Department Chemical Engineering, Carnegie Mellon University, 15213, Pittsburgh, PA, USA. jwmeade@andrew.cmu.edu.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Meade
authors.affiliation: Department Chemical Engineering, University of Pittsburgh, 15219, Pittsburgh, PA, USA. pab66@pitt.edu.
authors.forename: Patrick
authors.initials: P
authors.lastname: Bartlow
authors.affiliation: Microbiology & Molecular Genetics, University of Pittsburgh School of Medicine, 15219, Pittsburgh, PA, USA. rnt88@pitt.edu.
authors.forename: Ram Narayan
authors.initials: RN
authors.lastname: Trivedi
authors.affiliation: Microbiology & Molecular Genetics, University of Pittsburgh School of Medicine, 15219, Pittsburgh, PA, USA. paa13@pitt.edu.
authors.forename: Parvez
authors.initials: P
authors.lastname: Akhtar
authors.affiliation: Department Chemical Engineering, University of Pittsburgh, 15219, Pittsburgh, PA, USA. ataai@pitt.edu.
authors.forename: Mohammad M
authors.initials: MM
authors.lastname: Ataai
authors.affiliation: Microbiology & Molecular Genetics, University of Pittsburgh School of Medicine, 15219, Pittsburgh, PA, USA. Khan@pitt.edu.
authors.forename: Saleem A
authors.initials: SA
authors.lastname: Khan
authors.affiliation: Department Chemical Engineering, Carnegie Mellon University, 15213, Pittsburgh, PA, USA. md0q@andrew.cmu.edu.
authors.forename: Michael M
authors.initials: MM
authors.lastname: Domach
mesh_terms: D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D018628:Gene Dosage; D005947:Glucose / Q000378:metabolism; D007655:Ketoglutarate Dehydrogenase Complex / Q000378:metabolism; D008954:Models, Biological; D009154:Mutation; D010641:Phenotype; D010957:Plasmids / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D020543:Proteome; D012269:Ribosomal Proteins; D007655:Ketoglutarate Dehydrogenase Complex; D005947:Glucose