title: Dopamine modulates insulin release and is involved in the survival of rat pancreatic beta cells.
abstract: The local synthesis of dopamine and its effects on insulin release have been described in isolated islets. Thus, it may be accepted that dopamine exerts an auto-paracrine regulation of insulin secretion from pancreatic beta cells. The aim of the present study is to analyze whether dopamine is a regulator of the proliferation and apoptosis of rat pancreatic beta cells after glucose-stimulated insulin secretion. Glucose stimulated pancreatic islets obtained from male Wistar rats were cultured with 1 or 10 μM dopamine from 1 to 12 h. Insulin secretion was analyzed by RIA. The cellular proliferation rate of pancreatic islets and beta cells was studied with immunocytochemical double labelling for both insulin and PCNA (proliferating cell nuclear antigen), and active caspase-3 was detected to evaluate apoptosis. The secretion of insulin from isolated islets was significantly inhibited (p<0.01), by treatment with 1 and 10 μM dopamine, with no differences between either dose as early as 1 h after treatment. The percentage of insulin-positive cells in the islets decreased significantly (p<0.01) after 1 h of treatment up to 12 h. The proliferation rate of insulin-positive cells in the islets decreased significantly (p<0.01) following treatment with dopamine. Apoptosis in pancreatic islets and beta cells was increased by treatment with 1 and 10 μM dopamine along 12 h. In conclusion, these results suggest that dopamine could modulate the proliferation and apoptosis of pancreatic beta cells and that dopamine may be involved in the maintenance of pancreatic islets.
authors.affiliation: Department of Physiology and Pharmacology, Faculty of Medicine, University of Salamanca, Salamanca, Spain; Laboratory of Neuroendocrinology, Institute of Neurosciences of Castilla y León, and Laboratory of Neuroendocrinology and Obesity of IBSAL, University of Salamanca, Salamanca, Spain.
authors.forename: Maria Jose
authors.initials: MJ
authors.lastname: Garcia Barrado
authors.affiliation: Department of Physiology and Pharmacology, Faculty of Medicine, University of Salamanca, Salamanca, Spain; Laboratory of Neuroendocrinology, Institute of Neurosciences of Castilla y León, and Laboratory of Neuroendocrinology and Obesity of IBSAL, University of Salamanca, Salamanca, Spain.
authors.forename: Maria Carmen
authors.initials: MC
authors.lastname: Iglesias Osma
authors.affiliation: Department of Human Anatomy and Histology, Faculty of Medicine, University of Salamanca, Salamanca, Spain; Laboratory of Neuroendocrinology, Institute of Neurosciences of Castilla y León, and Laboratory of Neuroendocrinology and Obesity of IBSAL, University of Salamanca, Salamanca, Spain.
authors.forename: Enrique J
authors.initials: EJ
authors.lastname: Blanco
authors.affiliation: Department of Human Anatomy and Histology, Faculty of Medicine, University of Salamanca, Salamanca, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Carretero Hernández
authors.affiliation: Department of Physiology and Pharmacology, Faculty of Medicine, University of Salamanca, Salamanca, Spain.
authors.forename: Virginia
authors.initials: V
authors.lastname: Sánchez Robledo
authors.affiliation: Department of Physiology and Pharmacology, Faculty of Medicine, University of Salamanca, Salamanca, Spain.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Catalano Iniesta
authors.affiliation: Laboratory of Neuroendocrinology, Institute of Neurosciences of Castilla y León, and Laboratory of Neuroendocrinology and Obesity of IBSAL, University of Salamanca, Salamanca, Spain.
authors.forename: Sixto
authors.initials: S
authors.lastname: Carrero
authors.affiliation: Department of Human Anatomy and Histology, Faculty of Medicine, University of Salamanca, Salamanca, Spain; Laboratory of Neuroendocrinology, Institute of Neurosciences of Castilla y León, and Laboratory of Neuroendocrinology and Obesity of IBSAL, University of Salamanca, Salamanca, Spain.
authors.forename: Jose
authors.initials: J
authors.lastname: Carretero
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects*; D004298:Dopamine / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000187:drug effects* / Q000378:metabolism; D007515:Islets of Langerhans / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D007328:Insulin; D005947:Glucose; D004298:Dopamine