title: Identification of Entamoeba histolytica by Molecular Method in Surface Water of Rasht City, Iran.
abstract: BACKGROUND
  This study was conducted with the aim of determining surface water contamination with cysts of Entamoeba histolytica using PCR in Rasht City, Northern Iran.
  
  
  METHODS
  In this cross-sectional study, 49 water samples including 18 rivers and 6 wetlands were collected from different regions near the city of Rasht in autumn of 2012. After filtration using 0.22 μm nitrate cellulose membrane filters, the samples were examined using microscope and PCR method.
  
  
  RESULTS
  In microscopic examination, four samples of the 49 samples were positive for cysts of E. (histolytica / dispar / muschkovskii). By using PCR method and molecular analysis, one sample was positive for E. histolytica.
  
  
  CONCLUSIONS
  In the molecular analysis, contamination by E. histolytica was proved in the waters of Rasht City. Further investigations including more samples and necessary preparations must be applied to prevent contamination.
authors.affiliation: Dep. of Microbiology, Rasht Branch, Islamic Azad University, Rasht, Iran.
authors.forename: Arash
authors.initials: A
authors.lastname: Hemmati
authors.affiliation: Dep. of Veterinary, Rasht Branch, Islamic Azad University, Rasht, Iran.
authors.forename: Elham
authors.initials: E
authors.lastname: Hooshmand
authors.affiliation: Molecular Biology Research Center, School of Medicine, Baqiyatallah University, Tehran, Iran.
authors.forename: Mohammad Javad
authors.initials: MJ
authors.lastname: Hosseini
keywords: Entamoeba histolytica; Iran; PCR; Surface water