title: Prenatal dietary load of Maillard reaction products combined with postnatal Coca-Cola drinking affects metabolic status of female Wistar rats.
abstract: OBJECTIVE
  To assess the impact of prenatal exposure to Maillard reaction products (MRPs) -rich diet and postnatal Coca-Cola consumption on metabolic status of female rats. Diet rich in MRPs and consumption of saccharose/fructose sweetened soft drinks is presumed to impose increased risk of development of cardiometabolic afflictions, such as obesity or insulin resistance.
  
  
  METHODS
  At the first day of pregnancy, 9 female Wistar rats were randomized into two groups, pair-fed either with standard rat chow (MRP-) or MRPs-rich diet (MRP+). Offspring from each group of mothers was divided into two groups and given either water (Cola-) or Coca-Cola (Cola+) for drinking ad libitum for 18 days. Oral glucose tolerance test was performed, and circulating markers of inflammation, oxidative stress, glucose and lipid metabolism were assessed.
  
  
  RESULTS
  MRP+ groups had higher weight gain, significantly so in the MRP+/Cola- vs MRP-/Cola-. Both prenatal and postnatal intervention increased carboxymethyllysine levels and semicarbazide-sensitive amine oxidase activity, both significantly higher in MRP+/Cola + than in MRP-/Cola-. Total antioxidant capacity was lower in MRP+ groups, with significant decrease in MRP+/Cola + vs MRP-/Cola+. Rats drinking Coca-Cola had higher insulin, homeostatic model assessment of insulin resistance, heart rate, advanced oxidation of protein products, triacylglycerols, and oxidative stress markers measured as thiobarbituric acid reactive substances compared to rats drinking water, with no visible effect of MRPs-rich diet.
  
  
  CONCLUSIONS
  Metabolic status of rats was affected both by prenatal and postnatal dietary intervention. Our results suggest that combined effect of prenatal MRPs load and postnatal Coca-Cola drinking may play a role in development of metabolic disorders in later life.
authors.affiliation: Radana Gurecká, Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University, Sasinkova 4, 811 08 Bratislava, Slovakia, radana.kollarova@gmail.com.
authors.forename: Radana
authors.initials: R
authors.lastname: Gurecká
authors.forename: Ivana
authors.initials: I
authors.lastname: Koborová
authors.forename: Katarína
authors.initials: K
authors.lastname: Janšáková
authors.forename: Tamás
authors.initials: T
authors.lastname: Tábi
authors.forename: Éva
authors.initials: É
authors.lastname: Szökő
authors.forename: Veronika
authors.initials: V
authors.lastname: Somoza
authors.forename: Katarína
authors.initials: K
authors.lastname: Šebeková
authors.forename: Peter
authors.initials: P
authors.lastname: Celec
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D002253:Carbonated Beverages; D004032:Diet; D005260:Female; D005951:Glucose Tolerance Test; D050356:Lipid Metabolism; D015416:Maillard Reaction; D008659:Metabolic Diseases / Q000097:blood / Q000209:etiology*; D018384:Oxidative Stress; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000209:etiology*; D039401:Prenatal Nutritional Physiological Phenomena; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D017392:Thiobarbituric Acid Reactive Substances