title: [A new fluorescent analogue for the investigation of anandamide transport in cell cultures].
abstract: For the first time a new fluorescent analogue of anadamide incorporating BODIPY®-FL-fluorophore, attached to arachidonic acid via 2,2'-(ethylenedioxy)-bis(ethylenediamine), was prepared. Using rat glioma C6 cells it was demonstrated that the fluorescent analogue is a substrate of the cellular anandamide uptake system (Km 4.5 ± 0.9 µM, Vmax 20 ± 1 amol/(min x cell)).
authors.forename: N M
authors.initials: NM
authors.lastname: Gretskaia
authors.forename: M G
authors.initials: MG
authors.lastname: Akimov
authors.forename: V V
authors.initials: VV
authors.lastname: Bezuglov
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000737:chemistry; D001095:Arachidonic Acids / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D058948:Cell Tracking / Q000379:methods; D063388:Endocannabinoids / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D005910:Glioma / Q000737:chemistry / Q000378:metabolism*; D066298:In Vitro Techniques / Q000379:methods*; D053284:Polyunsaturated Alkamides / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D051381:Rats
chemical_list: D001095:Arachidonic Acids; D063388:Endocannabinoids; D005456:Fluorescent Dyes; D053284:Polyunsaturated Alkamides; D016718:Arachidonic Acid; C078814:anandamide