title: Remodeling Characteristics and Collagen Distributions of Biologic Scaffold Materials Biopsied From Postmastectomy Breast Reconstruction Sites.
abstract: OBJECTIVE
  The study purpose was to evaluate the associations between patient characteristics and the histologic remodeling scores of acellular dermal matrices (ADMs) biopsied from breast reconstruction sites in the first attempt to generate a multivariable risk prediction model of nonconstructive remodeling. It was hypothesized that host characteristics and surgical site assessments predict the degree of graft remodeling for ADMs used during breast reconstruction.
  
  
  METHODS
  The ADMs were biopsied from the breast reconstruction sites of n = 62 patients during a subsequent breast procedure, stained with hematoxylin-eosin, and evaluated according to a semi-quantitative scoring system for remodeling characteristics (cell types, cell infiltration, extracellular matrix deposition, scaffold degradation, fibrous encapsulation, and neovascularization) and a mean composite score. Biopsies were stained with Sirius Red and Fast Green, and analyzed to determine the collagen I:III ratio. Based on univariate analyses between subject clinical characteristics and the histologic remodeling scores, cohort variables were selected for multivariable regression models using a P value of 0.20 or less.
  
  
  RESULTS
  The composite score model yielded 3 variables: pack-year history, corticosteroid use, and radiation timing (r pseudo = 0.81). The model for collagen I yielded 2 variables: corticosteroid use and reason for reoperation (r pseudo = 0.78). The model for collagen III yielded 1 variable: reason for reoperation (r pseudo = 0.35).
  
  
  CONCLUSIONS
  These preliminary results constitute the first steps in generating a risk prediction model that predicts the patients and clinical circumstances most likely to experience nonconstructive remodeling of biologic grafts used to reconstruct the breast.
authors.affiliation: From the *Section of Minimally Invasive Surgery, Department of Surgery, †Division of Plastic and Reconstructive Surgery, Washington University School of Medicine, St. Louis, MO; ‡Department of Anatomic Pathology, Cleveland Clinic, Cleveland, OH; and §Department of Biostatistics, Washington University School of Medicine, St. Louis, MO.
authors.forename: Jaime A
authors.initials: JA
authors.lastname: Cavallo
authors.forename: Noopur
authors.initials: N
authors.lastname: Gangopadhyay
authors.forename: Jason
authors.initials: J
authors.lastname: Dudas
authors.forename: Andres A
authors.initials: AA
authors.lastname: Roma
authors.forename: Mateusz S
authors.initials: MS
authors.lastname: Jasielec
authors.forename: Jack
authors.initials: J
authors.lastname: Baty
authors.forename: Sara
authors.initials: S
authors.lastname: Baalman
authors.forename: Margaret M
authors.initials: MM
authors.lastname: Frisella
authors.forename: Marissa M
authors.initials: MM
authors.lastname: Tenenbaum
authors.forename: Terence M
authors.initials: TM
authors.lastname: Myckatyn
authors.forename: Brent D
authors.initials: BD
authors.lastname: Matthews
authors.forename: Corey R
authors.initials: CR
authors.lastname: Deeken
mesh_terms: D062088:Acellular Dermis; D000328:Adult; D000368:Aged; D001672:Biocompatible Materials; D001706:Biopsy; D001940:Breast / Q000473:pathology* / Q000601:surgery*; D003094:Collagen / Q000032:analysis; D005260:Female; D006801:Humans; D016462:Mammaplasty; D008408:Mastectomy; D008875:Middle Aged; D011446:Prospective Studies; D054457:Tissue Scaffolds; D055815:Young Adult
chemical_list: D001672:Biocompatible Materials; D003094:Collagen