title: Expression Profiles and Clinical Significance of MicroRNAs in Papillary Renal Cell Carcinoma: A STROBE-Compliant Observational Study.
abstract: Papillary renal cell carcinoma (pRCC) is the second most prevalent subtype of kidney cancers. In the current study, we analyzed the global microRNA (miRNA) expression profiles in pRCC, with the aim to evaluate the relationship of miRNA expression with the progression and prognosis of pRCC.A total of 163 treatment-naïve primary pRCC patients were identified from the Cancer Genome Atlas dataset and included in this retrospective observational study. The miRNA expression profiles were graded by tumor-node-metastasis information, and compared between histologic subtypes. Furthermore, the training-validation approach was applied to identify miRNAs of prognostic values, with the aid of Kaplan-Meier survival, and univariate and multivariate Cox regression analyses. Finally, the online DAVID (Database for Annotation, Visualization, and Integrated Discover) program was applied for the pathway enrichment analysis with the target genes of prognosis-associated miRNAs, which were predicted by 3 computational algorithms (PicTar, TargetScan, and Miranda).In the progression-related miRNA profiles, 26 miRNAs were selected for pathologic stage, 28 for pathologic T, 16 for lymph node status, 3 for metastasis status, and 32 for histologic types, respectively. In the training stage, the expression levels of 12 miRNAs (mir-134, mir-379, mir-127, mir-452, mir-199a, mir-200c, mir-141, mir-3074, mir-1468, mir-181c, mir-1180, and mir-34a) were significantly associated with patient survival, whereas mir-200c, mir-127, mir-34a, and mir-181c were identified by multivariate Cox regression analyses as potential independent prognostic factors in pRCC. Subsequently, mir-200c, mir-127, and mir-34a were confirmed to be significantly correlated with patient survival in the validation stage. Finally, target gene prediction analysis identified a total of 113 target genes for mir-200c, 37 for mir-127, and 180 for mir-34a, which further generated 15 molecular pathways.Our results identified the specific miRNAs associated with the progression and aggressiveness of pRCC, and 3 miRNAs (mir-200c, mir-127, and mir-34a) as promising prognostic factors of pRCC.
authors.affiliation: From the Department of Urology (YZG, LWX, ZX, RW, HX, TL, CCZ, ZKH, LHZ, JPW, WCL, JGZ, RPJ), Nanjing First Hospital, Nanjing Medical University, Nanjing; Department of Epidemiology and Biostatistics and Ministry of Education (MOE) Key Lab for Modern Toxicology (MZ, LGG), School of Public Health, Nanjing Medical University, Nanjing; Department of Urology (HL), The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou; Department of Urology (PY), The First Hospital of Nanchang, Nanchang University, Nanchang; Department of Pathology (YCZ, ZKH), Nanjing First Hospital, Nanjing Medical University, Nanjing; and Department of Urology (YZ), Xuzhou Third People's Hospital, Jiangsu University, Xuzhou, China.
authors.forename: Yu-Zheng
authors.initials: YZ
authors.lastname: Ge
authors.forename: Lu-Wei
authors.initials: LW
authors.lastname: Xu
authors.forename: Zheng
authors.initials: Z
authors.lastname: Xu
authors.forename: Ran
authors.initials: R
authors.lastname: Wu
authors.forename: Hui
authors.initials: H
authors.lastname: Xin
authors.forename: Meng
authors.initials: M
authors.lastname: Zhu
authors.forename: Tian-Ze
authors.initials: TZ
authors.lastname: Lu
authors.forename: Li-Guo
authors.initials: LG
authors.lastname: Geng
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
authors.forename: Chang-Cheng
authors.initials: CC
authors.lastname: Zhou
authors.forename: Peng
authors.initials: P
authors.lastname: Yu
authors.forename: You-Cai
authors.initials: YC
authors.lastname: Zhao
authors.forename: Zhi-Kai
authors.initials: ZK
authors.lastname: Hu
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Liu-Hua
authors.initials: LH
authors.lastname: Zhou
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Wu
authors.forename: Wen-Cheng
authors.initials: WC
authors.lastname: Li
authors.forename: Jia-Geng
authors.initials: JG
authors.lastname: Zhu
authors.forename: Rui-Peng
authors.initials: RP
authors.lastname: Jia
mesh_terms: D000367:Age Factors; D000368:Aged; D002292:Carcinoma, Renal Cell / Q000175:diagnosis* / Q000235:genetics*; D018450:Disease Progression; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007680:Kidney Neoplasms / Q000175:diagnosis* / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis*; D008875:Middle Aged; D011379:Prognosis; D012189:Retrospective Studies; D012737:Sex Factors
chemical_list: D035683:MicroRNAs