title: Dendrobium huoshanense polysaccharide prevents ethanol-induced liver injury in mice by metabolomic analysis.
abstract: The prevalence of alcohol consumption has increased in modern dietary life and alcoholic liver injury can follow. Dendrobium huoshanense polysaccharide (DHP) is a homogeneous polysaccharide isolated from Dendrobium huoshanense, which possesses hepatoprotection function. In this study, we investigated the metabolic profiles of serum and liver tissues extracts from control, ethanol-treated and DHP\ethanol-treated mice using a UHPLC/LTQ Orbitrap XL MS-based metabolomics approach. Our results indicated that DHP alleviated early steatosis and inflammation in liver histology and the metabolomic analysis of serum and hepatic tissue revealed that first, ethanol treatment mainly altered phosphatidylcholines (PCs) including PC (13:0) and phosphocholine, arachidonic acid metabolites including 20-ethyl PGF2α and amino acids including L-Proline; Second, DHP supplementation ameliorated the altered metabolic levels particularly involved in phosphocholine and L-Proline. These data suggested that DHP might restore the perturbed metabolism pathways by ethanol exposure to prevent the progression of alcoholic liver injury.
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, China; Department of Pharmacy, The First Affiliated Hospital, Anhui University of Traditional Chinese Medicine, Hefei, China.
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Wang
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, China. Electronic address: jianpingluo@hfut.edu.cn.
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Luo
authors.affiliation: Department of Biostatistics, University of Pittsburgh, Pittsburgh, PA, USA.
authors.forename: Rui
authors.initials: R
authors.lastname: Chen
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, China.
authors.forename: Xue-Qiang
authors.initials: XQ
authors.lastname: Zha
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, China.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Pan
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D031667:Dendrobium / Q000737:chemistry*; D004195:Disease Models, Animal; D000431:Ethanol / Q000009:adverse effects*; D008107:Liver Diseases / Q000188:drug therapy / Q000209:etiology* / Q000378:metabolism* / Q000473:pathology; D013058:Mass Spectrometry; D055442:Metabolome; D055432:Metabolomics / Q000379:methods; D051379:Mice; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D020011:Protective Agents / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D011134:Polysaccharides; D020011:Protective Agents; D000431:Ethanol
keywords: Alcoholic liver injury; Dendrobium huoshanense polysaccharide; Metabolomics