title: [Study on alkaloids of Corydalis ochotensis and their antitumor bioactivity].
abstract: OBJECTIVE
  To investigate the chemical constituents of Corydalis ochotensis and their antitumor bioactivity.
  
  
  METHODS
  The compounds were isolated by silica gel column chromatography and recrystallization. Their structures were identified by spectroscopic analysis (NMR) and physicochemical properties. Their cytotoxic activity was studied by MTT.
  
  
  RESULTS
  Six compounds were elucidated as protopine (1), ochotensimine (2), fumariline (3), sanguinarine (4), tetrahydroberberine (5) and berberine (6). Compound 1 had excellent inhibitory activity on HepG2, SW480 and A549 cells, and compound 4 had excellent inhibitory activity on Hep2, HepG2, SW480 and A549 cells.
  
  
  CONCLUSIONS
  Compounds 3, 4 and 5 are isolated from this plant for the first time; In the MTT antitumor experiments,compounds 1 and 4 show an antitumor activity.
authors.forename: Jia-jia
authors.initials: JJ
authors.lastname: Yu
authors.forename: Deng-li
authors.initials: DL
authors.lastname: Cong
authors.forename: Ying
authors.initials: Y
authors.lastname: Jiang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Chun-fang
authors.initials: CF
authors.lastname: Zhao
mesh_terms: D000470:Alkaloids / Q000302:isolation & purification / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D053119:Benzophenanthridines; D001599:Berberine / Q000031:analogs & derivatives; D001600:Berberine Alkaloids; D045744:Cell Line, Tumor / Q000187:drug effects; D031311:Corydalis / Q000737:chemistry*; D006801:Humans; D007546:Isoquinolines
chemical_list: D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D053119:Benzophenanthridines; D001600:Berberine Alkaloids; D007546:Isoquinolines; D001599:Berberine; C049095:fumariline; C005705:sanguinarine; C009093:protopine; C004645:canadine