title: Bioassay-Guided Chromatographic Isolation and Identification of Antibacterial Compounds from Artemisia annua L. That Inhibit Clostridium perfringens Growth.
abstract: Clostridium perfringens is the causative agent of necrotic enteritis leading to significant losses in the poultry industry. Dichloromethane and n-hexane extracts of aerial parts of Artemisia annua (Asteraceae) exhibited activity against C. perfringens with minimum inhibitory concentrations (MIC) of 185 and 270 μg/mL, respectively. Bioassay-guided fractionation of the extracts gave several active fractions (MIC between 75 and 600 μg/mL). Investigations of the most active fractions resulted in the isolation and characterization of the polyacetylene ponticaepoxide (MIC between 100 and 200 μg/mL) and (+)-threo-(5E)-trideca-1,5-dien-7,9,11-triyne-3,4-diol (MIC between 400 and 800 μg/mL), the flavonols chrysosplenol D (MIC between 200 and 400 μg/mL) and casticin (slight inhibition at 800 μg/mL), and 2,4-dihydroxy-6-methoxyacetophenone (slight inhibition at 800 μg/mL). Also, the coumarin scopoletin and the sesquiterpene lactone artemisinin were isolated from active fractions but showed no inhibition of C. perfringens growth at 800 and 2000 μg/mL, respectively. Fractions containing essential oil components with camphor constituting >60% did not show inhibition of C. perfringens up to 1600 μg/mL. Extracts and some active fractions showed higher antibacterial effect than individual bioactive compounds, suggesting that synergistic effects may underlie the observed antibacterial effect. The present study adds new valuable information on the antibacterial effect of A. annua against C. perfringens.
authors.forename: Elise
authors.initials: E
authors.lastname: Ivarsen
authors.affiliation: University of Southern Denmark, Department of Chemical Engineering, Biotechnology and Environmental Technology, Campusvej 55, DK-5230 Odense M, Denmark.
authors.forename: Xavier C
authors.initials: XC
authors.lastname: Fretté
authors.forename: Kathrine B
authors.initials: KB
authors.lastname: Christensen
authors.forename: Lars P
authors.initials: LP
authors.lastname: Christensen
authors.forename: Ricarda M
authors.initials: RM
authors.lastname: Engberg
authors.forename: Kai
authors.initials: K
authors.lastname: Grevsen
authors.forename: Anders
authors.initials: A
authors.lastname: Kjaer
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D031172:Artemisia annua / Q000378:metabolism*; D001681:Biological Assay; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003016:Clostridium perfringens / Q000187:drug effects* / Q000254:growth & development; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000032:analysis* / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts