title: Antiageing Mechanisms of a Standardized Supercritical CO 2 Preparation of Black Jack (Bidens pilosa L.) in Human Fibroblasts and Skin Fragments.
abstract: The use of topical retinoids to treat skin disorders and ageing can induce local reactions, while oral retinoids are potent teratogens and produce several unwanted effects. This way, efforts to explore complementary care resources should be supported. Based on this, we evaluate the antiageing effects of a supercritical CO2 extract from Bidens pilosa L. (BPE-CO2A) containing a standardized multicomponent mixture of phytol, linolenic, palmitic, linoleic, and oleic acids. BPE-CO2A was assessed for its effects on human dermal fibroblasts (TGF-β1 and FGF levels using ELISA; collagen, elastin, and glycosaminoglycan by colorimetric assays, and mRNA expression of RXR, RAR, and EGFr by qRT-PCR) and human skin fragments (RAR, RXR, collagen, elastin, and glycosaminoglycan by immunohistochemical analysis). Levels of extracellular matrix elements, TGF-β1 and FGF, and EGFr gene expression were significantly increased by BPE-CO2A. The modulation of RXR and RAR was positively demonstrated after the treatment with BPE-CO2A or phytol, a component of BPE-CO2A. The effects produced by BPE-CO2A were similar to or better than those produced by retinol and retinoic acid. The ability to stimulate extracellular matrix elements, increase growth factors, and modulate retinoid and rexinoid receptors provides a basis for the development of preparation containing BPE-CO2A as an antiageing/skin-repair agent.
authors.affiliation: Chemyunion Quimica Ltda, Avenida Independência 1501, 18087-101 Sorocaba, SP, Brazil.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Dieamant
authors.affiliation: Chemyunion Quimica Ltda, Avenida Independência 1501, 18087-101 Sorocaba, SP, Brazil.
authors.forename: Maria Del Carmen V
authors.initials: Mdel C
authors.lastname: Pereda
authors.affiliation: Chemyunion Quimica Ltda, Avenida Independência 1501, 18087-101 Sorocaba, SP, Brazil.
authors.forename: Cecília
authors.initials: C
authors.lastname: Nogueira
authors.affiliation: Chemyunion Quimica Ltda, Avenida Independência 1501, 18087-101 Sorocaba, SP, Brazil.
authors.forename: Samara
authors.initials: S
authors.lastname: Eberlin
authors.affiliation: Chemyunion Quimica Ltda, Avenida Independência 1501, 18087-101 Sorocaba, SP, Brazil.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Facchini
authors.affiliation: Laboratory of Phytomedicines, Pharmacology and Biotechnology (PhytoPharmaTech), Department of Pharmacology, Institute of Biosciences, Universidade Estadual Paulista (UNESP), 18618-000 Botucatu, SP, Brazil.
authors.forename: Juliana Tibério
authors.initials: JT
authors.lastname: Checon
authors.affiliation: Chemyunion Quimica Ltda, Avenida Independência 1501, 18087-101 Sorocaba, SP, Brazil.
authors.forename: Camila Kappke
authors.initials: CK
authors.lastname: Cesar
authors.affiliation: Chemyunion Quimica Ltda, Avenida Independência 1501, 18087-101 Sorocaba, SP, Brazil.
authors.forename: Lilian
authors.initials: L
authors.lastname: Mussi
authors.affiliation: Chemyunion Quimica Ltda, Avenida Independência 1501, 18087-101 Sorocaba, SP, Brazil.
authors.forename: Márcio Antonio
authors.initials: MA
authors.lastname: Polezel
authors.affiliation: Chemyunion Quimica Ltda, Avenida Independência 1501, 18087-101 Sorocaba, SP, Brazil.
authors.forename: Divino
authors.initials: D
authors.lastname: Martins-Oliveira
authors.affiliation: Laboratory of Phytomedicines, Pharmacology and Biotechnology (PhytoPharmaTech), Department of Pharmacology, Institute of Biosciences, Universidade Estadual Paulista (UNESP), 18618-000 Botucatu, SP, Brazil.
authors.forename: Luiz Claudio
authors.initials: LC
authors.lastname: Di Stasi