title: [[Expression of house dust mite allergens Der f1 and Der f2 in Nicotiana benthamiana leaves].
abstract: The class E immunoglobulins (IgE) is known to recognize conformational epitopes and therefore the native conformation of recombinant allergens is essential for their using in test-systems. Recombinant Dermatophagoides farinae house dust mite (HDM) allergens Der f1 and Der f2 were expressed in bacteria Escherichia coli and Nicotiana benthamiana plants. It has been shown that IgE in sera from children allergic to HDM recognizes Der f2 expressed both in E. coli and N. benthamiana. Mature form of Der f1 expressed in E. coli does not interact with IgE while the protein purified from N. benthamiana is able to recognize IgE as a native allergen.
authors.forename: D Iu
authors.initials: DIu
authors.lastname: Riazantsev
authors.forename: P E
authors.initials: PE
authors.lastname: Drobiazina
authors.forename: S V
authors.initials: SV
authors.lastname: Hlgatian
authors.forename: S K
authors.initials: SK
authors.lastname: Zavriev
authors.forename: E V
authors.initials: EV
authors.lastname: Svirshchevskaia
mesh_terms: D000485:Allergens / Q000235:genetics / Q000276:immunology / Q000627:therapeutic use*; D000595:Amino Acid Sequence; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000096:biosynthesis* / Q000276:immunology / Q000627:therapeutic use; D060829:Arthropod Proteins / Q000096:biosynthesis* / Q000276:immunology / Q000627:therapeutic use; D002675:Child, Preschool; D003546:Cysteine Endopeptidases / Q000096:biosynthesis* / Q000276:immunology / Q000627:therapeutic use; D040001:Dermatophagoides farinae / Q000276:immunology / Q000472:pathogenicity; D000939:Epitopes / Q000276:immunology*; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology*; D018515:Plant Leaves / Q000235:genetics; D039981:Pyroglyphidae / Q000235:genetics / Q000276:immunology; D014026:Nicotiana / Q000235:genetics
chemical_list: D000485:Allergens; D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; C451160:Dermatophagoides farinae antigen f 2; D000939:Epitopes; D007073:Immunoglobulin E; D003546:Cysteine Endopeptidases; C451159:Dermatophagoides farinae antigen f 1