title: The DNA load of six high-risk human papillomavirus types and its association with cervical lesions.
abstract: BACKGROUND
  Analysing human papillomavirus (HPV) viral load is important in determining the risk of developing cervical cancer (CC); most knowledge to date regarding HPV viral load and cervical lesions has been related to HPV-16. This study evaluated the association between the viral load of the six most prevalent high-risk viral types in Colombia and cervical intraepithelial neoplasia (CIN) frequency.
  
  
  METHODS
  114 women without CIN and 59 women having CIN confirmed by colposcopy, all of them positive by conventional PCR for HPV infection in the initial screening, were included in the study. Samples were tested for six high-risk HPV types to determine viral copy number by real-time PCR. Crude and adjusted odds ratios (ORa) were estimated for evaluating the association between each viral type's DNA load and the risk of cervical lesions occurring.
  
  
  RESULTS
  The highest viral loads were identified for HPV-33 in CIN patients and for HPV-31 in patients without lesions (9.33 HPV copies, 2.95 interquartile range (IQR); 9.41 HPV copies, 2.58 IQR). Lesions were more frequent in HPV-16 patients having a low viral load (3.53 ORa, 1.16-10.74 95%CI) compared to those having high HPV-16 load (2.62 ORa, 1.08-6.35 95%CI). High viral load in HPV-31 patients was associated with lower CIN frequency (0.34 ORa, 0.15-0.78 95%CI).
  
  
  CONCLUSIONS
  An association between HPV DNA load and CIN frequency was seen to be type-specific and may have depended on the duration of infection. This analysis has provided information for understanding the effect of HPV DNA load on cervical lesion development.
authors.affiliation: Molecular Biology and Immunology Department, Fundación Instituto de Inmunología de Colombia (FIDIC), Carrera 50#26-20, Bogotá, Colombia. lfda.drio@gmail.com.|School of Medicine and Health Sciences, Universidad del Rosario, Carrera 24#63C-69, Bogotá, Colombia. lfda.drio@gmail.com.
authors.forename: Luisa
authors.initials: L
authors.lastname: Del Río-Ospina
authors.affiliation: Molecular Biology and Immunology Department, Fundación Instituto de Inmunología de Colombia (FIDIC), Carrera 50#26-20, Bogotá, Colombia. sara_soto@fidic.org.co.|Faculty of Natural and Mathematical Sciences, Universidad del Rosario, Carrera 24#63C-69, Bogotá, Colombia. sara_soto@fidic.org.co.
authors.forename: Sara Cecilia
authors.initials: SC
authors.lastname: Soto-De León
authors.affiliation: Molecular Biology and Immunology Department, Fundación Instituto de Inmunología de Colombia (FIDIC), Carrera 50#26-20, Bogotá, Colombia. milena_camargo@fidic.org.co.|Faculty of Natural and Mathematical Sciences, Universidad del Rosario, Carrera 24#63C-69, Bogotá, Colombia. milena_camargo@fidic.org.co.
authors.forename: Milena
authors.initials: M
authors.lastname: Camargo
authors.affiliation: Molecular Biology and Immunology Department, Fundación Instituto de Inmunología de Colombia (FIDIC), Carrera 50#26-20, Bogotá, Colombia. darandmorper@gmail.com.|Faculty of Natural and Mathematical Sciences, Universidad del Rosario, Carrera 24#63C-69, Bogotá, Colombia. darandmorper@gmail.com.
authors.forename: Darwin Andrés
authors.initials: DA
authors.lastname: Moreno-Pérez
authors.affiliation: Molecular Biology and Immunology Department, Fundación Instituto de Inmunología de Colombia (FIDIC), Carrera 50#26-20, Bogotá, Colombia. rsanchezpe@unal.edu.co.|School of Medicine, Universidad Nacional de Colombia, Carrera 45#26-85, Bogotá, Colombia. rsanchezpe@unal.edu.co.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Sánchez
authors.affiliation: Mathematics Department, Universidad Pública de Navarra, Pamplona, Spain. aperez@unavarra.es.
authors.forename: Antonio
authors.initials: A
authors.lastname: Pérez-Prados
authors.affiliation: Molecular Biology and Immunology Department, Fundación Instituto de Inmunología de Colombia (FIDIC), Carrera 50#26-20, Bogotá, Colombia. mepatarr@gmail.com.|School of Medicine, Universidad Nacional de Colombia, Carrera 45#26-85, Bogotá, Colombia. mepatarr@gmail.com.
authors.forename: Manuel Elkin
authors.initials: ME
authors.lastname: Patarroyo
authors.affiliation: Molecular Biology and Immunology Department, Fundación Instituto de Inmunología de Colombia (FIDIC), Carrera 50#26-20, Bogotá, Colombia. mapatarr.fidic@gmail.com.|School of Medicine and Health Sciences, Universidad del Rosario, Carrera 24#63C-69, Bogotá, Colombia. mapatarr.fidic@gmail.com.
authors.forename: Manuel Alfonso
authors.initials: MA
authors.lastname: Patarroyo
mesh_terms: D000328:Adult; D052685:Alphapapillomavirus / Q000145:classification / Q000235:genetics*; D001706:Biopsy; D002584:Cervix Uteri / Q000473:pathology* / Q000821:virology*; D003105:Colombia / Q000453:epidemiology; D004279:DNA, Viral; D005260:Female; D006801:Humans; D008875:Middle Aged; D016017:Odds Ratio; D030361:Papillomavirus Infections / Q000150:complications* / Q000821:virology*; D012307:Risk Factors; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology / Q000209:etiology* / Q000473:pathology*; D019562:Viral Load; D055815:Young Adult; D002578:Uterine Cervical Dysplasia / Q000453:epidemiology / Q000209:etiology / Q000473:pathology
chemical_list: D004279:DNA, Viral