title: [Analysis on genotype distribution and mutation of major hydroponic region of hepatitis B virus in Liaoning].
abstract: OBJECTIVE
  To understand the genotype and the mutation of amino acid (aa) in the major hydrophilic region (MHR) of hepatitis B virus (HBV) among the hepatitis B cases under surveillance in Liaoning province.
  
  
  METHODS
  The serum samples were collected from hepatitis B cases under surveillance in Liaoning. The complete S gene of HBV were amplified and sequenced. The aa sequences were analyzed with bioinformatics software.
  
  
  RESULTS
  A total of 81 sequences of S gene of HBV were ontained, including 7 sequences of genotype B (8.64%), 70 sequences of genotype C (86.42%) and 4 sequences of genotype D (4.94%). The mutation rate of aa of MHR of S gene was 4.87%. The mutation rate of T126I was highest (8.64%). The overall prevalence of mutant strain of MHR was 49.38% (40/81), and it was 42.86% for genotype B (3/7), 47.14% for genotype C (33/70) and 100% for genotype D (4/4). Statistical analysis revealed that there was no age, sex, genotypes and anti-HBc IgM (±) specific significant differences in aa mutant strains prevalence, while the difference between ALT abnormal group (ALT ≥ 43 IU/L) and ALT normal group (ALT<43 IU/L) was significant (P < 0.05).
  
  
  CONCLUSIONS
  Among the hepatitis B cases under surveillance in Liaoning, HBV genotype C predominant, followed by genotype B and genotype D. The mutation of aa in MHR of HBV detected in this study was consistent with previous research results. It is necessary to strengthen the surveillance for HBV mutation to provide accurate information for the development of hepatitis B prevention and control measures.
authors.affiliation: National Institute for Viral Disease Control and Prevention.
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhang
authors.affiliation: National Institute for Viral Disease Control and Prevention.
authors.forename: Liping
authors.initials: L
authors.lastname: Shen
authors.affiliation: National Immunization Programme, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Miao
authors.affiliation: Liaoning Provincial Center for Disease Control and Prevention.
authors.forename: Yi
authors.initials: Y
authors.lastname: Ren
authors.affiliation: National Institute for Viral Disease Control and Prevention.
authors.forename: Wenting
authors.initials: W
authors.lastname: Zhou
authors.affiliation: National Institute for Viral Disease Control and Prevention.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.affiliation: National Institute for Viral Disease Control and Prevention.
authors.forename: Shengli
authors.initials: S
authors.lastname: Bi
authors.affiliation: National Immunization Programme, Chinese Center for Disease Control and Prevention, Beijing 102206, China. Email: wfznip2@163.com.
authors.forename: Fuzhen
authors.initials: F
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D002681:China; D004279:DNA, Viral; D005838:Genotype; D006509:Hepatitis B / Q000235:genetics*; D006514:Hepatitis B Surface Antigens; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D018527:Hydroponics; D009154:Mutation; D015995:Prevalence
chemical_list: D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens