title: Interleukin-33/ST2 system attenuates aldosterone-induced adipogenesis and inflammation.
abstract: Interleukin-33 (IL-33) but not soluble ST2 (sST2) exerts anti-inflammatory and protective effects in several tissues. Aldosterone, a proinflammatory mediator which promotes adipogenesis, is elevated in obese patients. The aim of this study was to investigate the interactions between IL-33/ST2 system and Aldosterone in adipose tissue. Rats fed a high fat diet presented increased sST2 expression, diminished IL-33/sST2 ratio and enhanced levels of differentiation and inflammation in adipose tissue as compared to controls. A similar pattern was observed in adipose tissue from C57BL/6 Aldosterone-treated mice. In both animal models, Aldosterone was correlated with sST2. Treatment of 3T3-L1 adipocytes with IL-33 delayed adipocyte differentiation diminished lipid accumulation and decreased inflammation. Aldosterone decreased IL-33 and increased sST2 expressions in differentiated adipocytes. Aldosterone-induced adipocyte differentiation and inflammation were blocked by IL-33 treatment, but sST2 did not exert any effects. The crosstalk between IL-33/ST2 and Aldosterone could be relevant in the metabolic consequences of obesity.
authors.affiliation: Cardiovascular Translational Research, NavarraBiomed (Fundación Miguel Servet), Pamplona, Spain.
authors.forename: Ernesto
authors.initials: E
authors.lastname: Martínez-Martínez
authors.affiliation: Department of Physiology, School of Medicine, Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM), Universidad Complutense, Madrid, Spain.
authors.forename: Victoria
authors.initials: V
authors.lastname: Cachofeiro
authors.affiliation: INSERM, Centre d'Investigations Cliniques- Plurithématique 1433, UMR 1116 Université de Lorraine and CHU de Nancy, Vandoeuvre-lès-Nancy, France.
authors.forename: Elodie
authors.initials: E
authors.lastname: Rousseau
authors.affiliation: Cardiovascular Translational Research, NavarraBiomed (Fundación Miguel Servet), Pamplona, Spain; Heart Area, Hospital de Navarra, Pamplona, Spain.
authors.forename: Virginia
authors.initials: V
authors.lastname: Álvarez
authors.affiliation: INSERM, Centre d'Investigations Cliniques- Plurithématique 1433, UMR 1116 Université de Lorraine and CHU de Nancy, Vandoeuvre-lès-Nancy, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Calvier
authors.affiliation: Cardiovascular Translational Research, NavarraBiomed (Fundación Miguel Servet), Pamplona, Spain.
authors.forename: Amaya
authors.initials: A
authors.lastname: Fernández-Celis
authors.affiliation: INSERM, Centre d'Investigations Cliniques- Plurithématique 1433, UMR 1116 Université de Lorraine and CHU de Nancy, Vandoeuvre-lès-Nancy, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Leroy
authors.affiliation: Department of Physiology, School of Medicine, Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM), Universidad Complutense, Madrid, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Miana
authors.affiliation: Department of Physiology, School of Medicine, Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM), Universidad Complutense, Madrid, Spain.
authors.forename: Raquel
authors.initials: R
authors.lastname: Jurado-López
authors.affiliation: Department of Pharmacology, Universidad Autónoma de Madrid, Spain.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Briones
authors.affiliation: INSERM, Centre d'Investigations Cliniques- Plurithématique 1433, UMR 1116 Université de Lorraine and CHU de Nancy, Vandoeuvre-lès-Nancy, France; INSERM UMR 872 Team 1, Centre de Recherche des Cordeliers, University Pierre and Marie Curie, Paris, France.
authors.forename: Frederic
authors.initials: F
authors.lastname: Jaisser
authors.affiliation: INSERM, Centre d'Investigations Cliniques- Plurithématique 1433, UMR 1116 Université de Lorraine and CHU de Nancy, Vandoeuvre-lès-Nancy, France.
authors.forename: Faiez
authors.initials: F
authors.lastname: Zannad
authors.affiliation: INSERM, Centre d'Investigations Cliniques- Plurithématique 1433, UMR 1116 Université de Lorraine and CHU de Nancy, Vandoeuvre-lès-Nancy, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Rossignol
authors.affiliation: Cardiovascular Translational Research, NavarraBiomed (Fundación Miguel Servet), Pamplona, Spain; INSERM, Centre d'Investigations Cliniques- Plurithématique 1433, UMR 1116 Université de Lorraine and CHU de Nancy, Vandoeuvre-lès-Nancy, France. Electronic address: natalia.lopez.andres@navarra.es.
authors.forename: Natalia
authors.initials: N
authors.lastname: López-Andrés
mesh_terms: D041721:3T3-L1 Cells; D050156:Adipogenesis / Q000187:drug effects / Q000502:physiology*; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000450:Aldosterone / Q000494:pharmacology*; D000818:Animals; D059305:Diet, High-Fat; D007249:Inflammation / Q000378:metabolism; D000072179:Interleukin-1 Receptor-Like 1 Protein; D000067596:Interleukin-33 / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018123:Receptors, Interleukin / Q000378:metabolism*
chemical_list: C486189:Il1rl1 protein, mouse; D000072179:Interleukin-1 Receptor-Like 1 Protein; D000067596:Interleukin-33; D018123:Receptors, Interleukin; D000450:Aldosterone
keywords: Adipocyte differentiation; Adipose tissue; Aldosterone; IL-33/ST2 system