title: Organelle specific imaging in live cells and immuno-labeling using resonance Raman probe.
abstract: Raman microspectroscopy is one of the most powerful tools in molecular sensing, offering a non-invasive and comprehensive characterization of the intracellular environment. To analyze and monitor molecular content in specific cellular compartments, different parts of cellular architecture must be unambiguously identified to guide Raman image/spectra acquisition. In this regards, the development of Raman molecular probes, producing spectrally distinct and intense signal is of outmost practical importance. Here we report on a new generation of Raman molecular probes, designed for application in live cells and immuno-labeling, capable of providing unprecedentedly high detection sensitivity through Resonance Raman (RR) enhancement. In contrast to existing Raman markers, the proposed RR reporter is designed to produce RR enhancement under excitation in the visible spectral range, far away from absorption of cellular biomolecules. We show that this concept allows for facile identification of labeled cellular domains, simultaneously with mapping of the macromolecules using spontaneous Raman technique. We demonstrate the breakthrough potential of these RR probes for selective labeling and rapid Raman imaging of membranes as well as mitochondria in live cells. We also show that these resonant Raman probes open the way for Raman-based intracellular immuno-labeling.
authors.affiliation: Institute for Lasers, Photonics and Biophotonics, State University of New York, University at Buffalo, Buffalo, NY 14260, USA; School of Information Science and Engineering, Lanzhou University, Lanzhou, Gansu 730000, China.
authors.forename: Yuee
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute for Lasers, Photonics and Biophotonics, State University of New York, University at Buffalo, Buffalo, NY 14260, USA; Center for Theragnosis, Korea Institute of Science and Technology, Seoul 136-791, Republic of Korea.
authors.forename: Jeongyun
authors.initials: J
authors.lastname: Heo
authors.affiliation: Institute for Lasers, Photonics and Biophotonics, State University of New York, University at Buffalo, Buffalo, NY 14260, USA.
authors.forename: Chang-Keun
authors.initials: CK
authors.lastname: Lim
authors.affiliation: Institute for Lasers, Photonics and Biophotonics, State University of New York, University at Buffalo, Buffalo, NY 14260, USA.
authors.forename: Artem
authors.initials: A
authors.lastname: Pliss
authors.affiliation: Institute for Lasers, Photonics and Biophotonics, State University of New York, University at Buffalo, Buffalo, NY 14260, USA.
authors.forename: Aliaksandr V
authors.initials: AV
authors.lastname: Kachynski
authors.affiliation: Institute for Lasers, Photonics and Biophotonics, State University of New York, University at Buffalo, Buffalo, NY 14260, USA.
authors.forename: Andrey N
authors.initials: AN
authors.lastname: Kuzmin
authors.affiliation: Center for Theragnosis, Korea Institute of Science and Technology, Seoul 136-791, Republic of Korea. Electronic address: sehoonkim@kist.re.kr.
authors.forename: Sehoon
authors.initials: S
authors.lastname: Kim
authors.affiliation: Institute for Lasers, Photonics and Biophotonics, State University of New York, University at Buffalo, Buffalo, NY 14260, USA; Department of Chemistry, Korea University, Seoul 136-701, Republic of Korea. Electronic address: pnprasad@buffalo.edu.
authors.forename: Paras N
authors.initials: PN
authors.lastname: Prasad
mesh_terms: D015335:Molecular Probes; D015388:Organelles / Q000378:metabolism*; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D015335:Molecular Probes
keywords: Immuno-labeling; Intracellular imaging; Raman spectroscopy; Resonance Raman probe; Vibrational spectroscopy