title: [Recombinant granulocyte-colony stimulating factor (filgrastim): optimization of conditions of isolation and purification from inclusion body].
abstract: We developed a unified process platform for two recombinant human GCSF medicines--one with the non-prolonged and the other with prolonged action. This unified technology led to a simpler and cheaper production while introduction of the additional pegylation stage to the technological line eased obtaining of the medicines with different action and allowed to standardize technological process documenting according to GMP requirements.
authors.forename: N V
authors.initials: NV
authors.lastname: Kononova
authors.forename: A V
authors.initials: AV
authors.lastname: Iakovlev
authors.forename: A M
authors.initials: AM
authors.lastname: Zhuravko
authors.forename: N N
authors.initials: NN
authors.lastname: Pankeev
authors.forename: S V
authors.initials: SV
authors.lastname: Minaev
authors.forename: A I
authors.initials: AI
authors.lastname: Bobruskin
authors.forename: V A
authors.initials: VA
authors.lastname: Mart'ianov
mesh_terms: D064420:Drug-Related Side Effects and Adverse Reactions; D064147:Febrile Neutropenia / Q000139:chemically induced / Q000473:pathology; D000069585:Filgrastim; D016179:Granulocyte Colony-Stimulating Factor / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000627:therapeutic use; D006801:Humans; D002479:Inclusion Bodies / Q000737:chemistry*; D009369:Neoplasms / Q000150:complications / Q000188:drug therapy* / Q000473:pathology; D011092:Polyethylene Glycols / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000627:therapeutic use
chemical_list: D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D011092:Polyethylene Glycols; D000069585:Filgrastim