title: Evaluation of leishmanicidal and trypanocidal activities of phenolic compounds from Calea uniflora Less.
abstract: The phytochemical study of Calea uniflora led to the isolation of nine phenolic compounds identified as noreugenin (1), ethyl caffeate (2), a mixture of butein (3) + orobol (4), α-hydroxy-butein (5), caffeic acid (6), butein 4'-O-glucopyranosyl (7), quercetin 3-O-glucopyranosyl (8) and 3,5-di-O-caffeoylquinic acid (9). The chemical identity of the isolates was established on the basis of NMR and physical data. The chemical shifts of 5 and 7 have been reassigned and all the isolates were tested against Leishmania amazonensis and Trypanosoma cruzi amastigotes. None of the metabolites showed promising leishmanicidal activity. However, 2 and the mixture of 3 and 4 demonstrated interesting trypanocidal effect with IC50 values of 18.27 and 26.53 μM, respectively. Besides, these compounds did not present cytotoxic effect towards THP-1 cells, and compound 2 was 3.5-fold more selective than the mixture of 3+4.
authors.affiliation: a Departamento de Ciências Farmacêuticas, CCS , Universidade Federal de Santa Catarina (UFSC) , Florianópolis , SC , Brazil.
authors.forename: Tamires C
authors.initials: TC
authors.lastname: Lima
authors.affiliation: a Departamento de Ciências Farmacêuticas, CCS , Universidade Federal de Santa Catarina (UFSC) , Florianópolis , SC , Brazil.
authors.forename: Rafaela J
authors.initials: RJ
authors.lastname: Souza
authors.affiliation: b Departamento de Química , Universidade Federal do Paraná (UFPR) , Curitiba , PR , Brazil.
authors.forename: Alan D C
authors.initials: AD
authors.lastname: Santos
authors.affiliation: c Departamento de Microbiologia e Parasitologia, CCB , Universidade Federal de Santa Catarina (UFSC) , Florianópolis , SC , Brazil.
authors.forename: Milene H
authors.initials: MH
authors.lastname: Moraes
authors.affiliation: c Departamento de Microbiologia e Parasitologia, CCB , Universidade Federal de Santa Catarina (UFSC) , Florianópolis , SC , Brazil.
authors.forename: Nicole E
authors.initials: NE
authors.lastname: Biondo
authors.affiliation: b Departamento de Química , Universidade Federal do Paraná (UFPR) , Curitiba , PR , Brazil.
authors.forename: Andersson
authors.initials: A
authors.lastname: Barison
authors.affiliation: c Departamento de Microbiologia e Parasitologia, CCB , Universidade Federal de Santa Catarina (UFSC) , Florianópolis , SC , Brazil.
authors.forename: Mário
authors.initials: M
authors.lastname: Steindel
authors.affiliation: a Departamento de Ciências Farmacêuticas, CCS , Universidade Federal de Santa Catarina (UFSC) , Florianópolis , SC , Brazil.
authors.forename: Maique W
authors.initials: MW
authors.lastname: Biavatti
mesh_terms: D000818:Animals; D000977:Antiparasitic Agents / Q000494:pharmacology*; D019659:Asteraceae / Q000737:chemistry*; D006801:Humans; D007894:Leishmania mexicana / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D010636:Phenols / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D014344:Trypanocidal Agents / Q000494:pharmacology*; D014349:Trypanosoma cruzi / Q000187:drug effects
chemical_list: D000977:Antiparasitic Agents; D010636:Phenols; D014344:Trypanocidal Agents
keywords: Calea uniflora; leishmanicidal; phenolic compounds; trypanocidal