title: Determination of trifolirhizin in rat plasma by UPLC: Application to a pharmacokinetic study.
abstract: In this study, a simple, sensitive, and robust analytical method based on ultra-performance liquid chromatography (UPLC) has been developed for the determination of trifolirhizin in rat plasma using pirfenidone as internal standard (IS). After sample preparation by a simple liquid-liquid extraction, chromatography was performed on an Acquity UPLC BEH C18 column (2.1mm×50mm, 1.7μm particle size) and ultraviolet detection set at a wavelength of 366nm. The method was linear over the concentration range 25-1000ng/mL with a lower limit of quantification (LLOQ) of 25ng/mL. Inter- and intra-day precision (RSD%) were all within 10.2% and the accuracy (RE%) was equal or lower than 9.3%. The recovery was in the range of 78.5-86.4% for trifolirhizin and 87.4% for IS. Stability studies showed that trifolirhizin was stable under a variety of storage conditions. The method was successfully applied to a pharmacokinetic study involving oral administration of trifolirhizin to rats.
authors.affiliation: The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325027, China.
authors.forename: Kong-hai
authors.initials: KH
authors.lastname: Ni
authors.affiliation: The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Zheng-de
authors.initials: ZD
authors.lastname: Wen
authors.affiliation: The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Xin-ce
authors.initials: XC
authors.lastname: Huang
authors.affiliation: School of Pharmacy, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Chen-xi
authors.initials: CX
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Tian-tian
authors.initials: TT
authors.lastname: Ye
authors.affiliation: School of Pharmacy, Wenzhou Medical University, Wenzhou 325035, China. Electronic address: hgx@wmu.edu.cn.
authors.forename: Guo-xin
authors.initials: GX
authors.lastname: Hu
authors.affiliation: The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China. Electronic address: wzzhoumt@163.com.
authors.forename: Meng-tao
authors.initials: MT
authors.lastname: Zhou
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D005960:Glucosides / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D006576:Heterocyclic Compounds, 4 or More Rings / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D016014:Linear Models; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D005960:Glucosides; D006576:Heterocyclic Compounds, 4 or More Rings; C526230:trifolirhizin
keywords: Pharmacokinetic; Rat plasma; Trifolirhizin; UPLC