title: Functionalization of cobalt porphyrin-phospholipid bilayers with his-tagged ligands and antigens.
abstract: Methods to attach polypeptides to lipid bilayers are often indirect and ineffective, and can represent a substantial bottleneck in the formation of functionalized lipid-based materials. Although the polyhistidine tag (his-tag) has been transformative in its simplicity and efficacy in binding to immobilized metals, the successful application of this approach has been challenging in physiological settings. Here we show that lipid bilayers containing porphyrin-phospholipid conjugates that are chelated with cobalt, but not with other metals, can effectively capture his-tagged proteins and peptides. The binding follows a Co(II) to Co(III) transition and occurs within the sheltered hydrophobic bilayer, resulting in an essentially irreversible attachment in serum or in a million fold excess of competing imidazole. Using this approach we anchored homing peptides into the bilayer of preformed and cargo-loaded liposomes to enable tumour targeting without disrupting the bilayer integrity. As a further demonstration, a synthetic protein fragment derived from the human immunodeficiency virus was bound to immunogenic liposomes for potent antibody generation for an otherwise non-antigenic peptide.
authors.affiliation: 1] Department of Biomedical Engineering, University at Buffalo, State University of New York, Buffalo, New York 14260, USA [2] Department of Chemical and Biological Engineering, University at Buffalo, State University of New York. Buffalo, New York 14260, USA.
authors.forename: Shuai
authors.initials: S
authors.lastname: Shao
authors.affiliation: Department of Biomedical Engineering, University at Buffalo, State University of New York, Buffalo, New York 14260, USA.
authors.forename: Jumin
authors.initials: J
authors.lastname: Geng
authors.affiliation: Department of Microbiology and Immunology, University at Buffalo, State University of New York, Buffalo, New York 14260, USA.
authors.forename: Hyun
authors.initials: H
authors.lastname: Ah Yi
authors.affiliation: Department of Chemical and Biological Engineering, University at Buffalo, State University of New York. Buffalo, New York 14260, USA.
authors.forename: Shobhit
authors.identifier: 0000000345749261
authors.initials: S
authors.lastname: Gogia
authors.affiliation: Department of Chemical and Biological Engineering, University at Buffalo, State University of New York. Buffalo, New York 14260, USA.
authors.forename: Sriram
authors.initials: S
authors.lastname: Neelamegham
authors.affiliation: Department of Microbiology and Immunology, University at Buffalo, State University of New York, Buffalo, New York 14260, USA.
authors.forename: Amy
authors.initials: A
authors.lastname: Jacobs
authors.affiliation: 1] Department of Biomedical Engineering, University at Buffalo, State University of New York, Buffalo, New York 14260, USA [2] Department of Chemical and Biological Engineering, University at Buffalo, State University of New York. Buffalo, New York 14260, USA.
authors.forename: Jonathan F
authors.initials: JF
authors.lastname: Lovell
mesh_terms: D003035:Cobalt / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes; D010743:Phospholipids / Q000737:chemistry*; D011166:Porphyrins / Q000737:chemistry*
chemical_list: D008051:Lipid Bilayers; D008081:Liposomes; D010743:Phospholipids; D011166:Porphyrins; D003035:Cobalt