title: Molecular subtypification of human papillomavirus in male adult individuals with recurrent respiratory papillomatosis.
abstract: OBJECTIVE
  This study aimed to identify the isotype of human papillomavirus (HPV) in fresh tissue samples of 35 male adults with adult recurrent adult respiratory papillomatosis which may be important to define the precise etiology of the disease, and determine the therapeutic and prophylactic measures.
  
  
  METHODS
  A total of 35 adult male patients diagnosed with active RRP who have been treated for several years were included in the study. DNA of patients was extracted from fresh biological samples and analyzed by PCR and a Linear Array® HPV Genotyping system.
  
  
  RESULTS
  Most cases (95%) corresponded to adult-onset of RRP. A questionnaire was applied to obtain demographic and clinical data. Using a PCR-based detection system all patients showed the presence of HPV; 80% were positive for HPV-6, 8% for HPV-11 and one for HPV-16.
  
  
  CONCLUSIONS
  Most patients presented HPV-6 and consequently it was not feasible to correlate clinical and demographic characteristics with viral type. Besides, co-infections were not evident. This knowledge may be relevant to delineate therapeutic and preventive measures.
authors.affiliation: Laboratorio de Biología Molecular y Virología, Instituto Nacional de Perinatología, Secretaría de Salud, México, D.F., Mexico.
authors.forename: Carmen S
authors.initials: CS
authors.lastname: García-Romero
authors.affiliation: Servicio de Otorrinolaringología, Centro Médico Nacional "20 de Noviembre", Instituto de Seguridad y Servicios Sociales de los Trabajadores del Estado, México, D.F., Mexico.
authors.forename: Matsuharu
authors.initials: M
authors.lastname: Akaki-Caballero
authors.affiliation: Servicio de Otorrinolaringología, Centro Médico Nacional "20 de Noviembre", Instituto de Seguridad y Servicios Sociales de los Trabajadores del Estado, México, D.F., Mexico.
authors.forename: Ana G
authors.initials: AG
authors.lastname: Saavedra-Mendoza
authors.affiliation: Servicio de Otorrinolaringología, Centro Médico Nacional "20 de Noviembre", Instituto de Seguridad y Servicios Sociales de los Trabajadores del Estado, México, D.F., Mexico.
authors.forename: Ana K
authors.initials: AK
authors.lastname: Guzmán-Romero
authors.affiliation: Unidad de Investigación en Obesidad, Facultad de Medicina, Universidad Nacional Autónoma de México, México, D.F., Mexico.
authors.forename: Patricia
authors.initials: P
authors.lastname: Canto
authors.affiliation: Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional & Subdirección de Enseñanza e Investigación, Centro Médico Nacional "20 de Noviembre", Instituto de Seguridad y Servicios Sociales de los Trabajadores del Estado, México, D.F., Mexico. Electronic address: rmcoralv@gmail.com.
authors.forename: Ramón M
authors.initials: RM
authors.lastname: Coral-Vázquez
mesh_terms: D000328:Adult; D000368:Aged; D004279:DNA, Viral / Q000235:genetics*; D005838:Genotype; D052140:Human papillomavirus 11 / Q000235:genetics* / Q000302:isolation & purification; D052162:Human papillomavirus 16 / Q000235:genetics* / Q000302:isolation & purification; D052163:Human papillomavirus 6 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008800:Mexico; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000821:virology*; D016133:Polymerase Chain Reaction; D012141:Respiratory Tract Infections / Q000821:virology*; D014827:Vocal Cords / Q000821:virology; D055815:Young Adult
chemical_list: D004279:DNA, Viral
keywords: Adult; Human papillomavirus; Male; Mexico; Recurrent papillomatosis