title: [Curcuma wenyujin Diterpenoid compound C fought against gastric cancer: an experimental study].
abstract: OBJECTIVE
  To study the role of nuclear factor (NF)-kappaB pathway with p38MAPK in Curcuma wenyujin diterpenoid compound C (CDCC) fighting against inflammation and inducing gastric cancer cell apoptosis by stimulating gastric cell SGC7901 with tumor necrosis factor-alpha (TNF-alpha).
  
  
  METHODS
  Human umbilical vein endothelial cells (HUVECs) and human gastric cancer SGC-7901 cells were in vitro acted by CDCC in different concentrations at different time points. Their growth inhibition ratios were measured by MTT assay. The apoptosis rate of gastric cancer cells was detected by Annexin V-FITC/PI double staining. Nuclear translocation of p65 was detected by cell immunofluorescence. Expression levels of p38MAPK/P-p38MAPK, p65/P-p65, and Caspase 3/P-Caspase 3 were measured by Western blot.
  
  
  RESULTS
  CDCC had significant inhibitory effect on the proliferation of SGC-7901. It also could effectively induce the apoptosis of gastric cancer cell SGC-7901. It also could reduce nuclear translocation of p65 in gastric cancer cell SGC-7901. Results of Western blot indicated that expression levels of p38MAPK and p65 were reduced and the expression level of Caspase-3 was elevated along with increased concentrations of CDCC (P<0.05).
  
  
  CONCLUSIONS
  Apoptosis executive protein Caspase 3 activated by regulating p65 via p38MAPK might be one of possible mechanisms for CDCC fighting against inflammation and gastric cancer.
authors.forename: Hai-feng
authors.initials: HF
authors.lastname: Jin
authors.forename: Bin
authors.initials: B
authors.lastname: Lu
authors.forename: Jin-feng
authors.initials: JF
authors.lastname: Dai
authors.forename: Gui-qin
authors.initials: GQ
authors.lastname: Sheng
mesh_terms: D017209:Apoptosis; D053148:Caspase 3; D045744:Cell Line, Tumor; D030024:Curcuma; D004224:Diterpenes; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D006801:Humans; D016328:NF-kappa B; D013274:Stomach Neoplasms / Q000188:drug therapy*; D014409:Tumor Necrosis Factor-alpha
chemical_list: D004224:Diterpenes; D004365:Drugs, Chinese Herbal; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D053148:Caspase 3