title: Diversity of Class 1 Integron Gene Cassette Rearrangements Selected under Antibiotic Pressure.
abstract: Integrons are bacterial genetic elements able to capture and express genes contained within mobile gene cassettes. Gene cassettes are expressed via a Pc promoter and can be excised from or integrated into the integron by integrase IntI. Although the mechanisms of gene cassette integration and excision are well known, the kinetics and modes of gene cassette shuffling leading to new gene cassette arrays remain puzzling. It has been proposed that under antibiotic selective pressure, IntI-mediated rearrangements can generate integron variants in which a weakly expressed gene cassette moves closer to Pc, thus leading to higher-level resistance. To test this hypothesis, we used an integron with four gene cassettes, intI1-aac(6')-Ib-dfrA15-aadA1-catB9, and applied selective pressure with chloramphenicol, resistance to which is encoded by catB9. Experiments were performed with three different Pc variants corresponding to three IntI1 variants. All three integrases, even when not overexpressed, were able to bring catB9 closer to Pc via excision of the dfrA15 and aadA1 gene cassettes, allowing their host bacteria to adapt to antibiotic pressure and to grow at high chloramphenicol concentrations. Integrase IntI1(R32_H39), reported to have the highest recombination activity, was able, when overexpressed, to trigger multiple gene cassette rearrangements. Although we observed a wide variety of rearrangements with catB9 moving closer to Pc and leading to higher chloramphenicol resistance, "cut-and-paste" relocalization of catB9 to the first position was not detected. Our results suggest that gene cassette rearrangements via excision are probably less cost-effective than excision and integration of a distal gene cassette closer to Pc.
  
  
  OBJECTIVE
  Integrons are bacterial genetic elements able to capture and express gene cassettes. Gene cassettes are expressed via a Pc promoter; the closer they are to Pc, the more strongly they are expressed. Gene cassettes can be excised from or integrated into the integron by integrase IntI. The kinetics and modes of gene cassette shuffling, leading to new gene cassette arrays remain puzzling. We used an integron with 4 antibiotic resistance gene cassettes and applied selective pressure with the antibiotic for which resistance was encoded by cassette 4. All IntI variants were able to bring cassette 4 closer to Pc. Rearrangements occur via excision of the previous gene cassettes instead of cut-and-paste relocalization of the fourth gene cassette.
authors.affiliation: INSERM, U1092, Limoges, France.|Université de Limoges, UMR-S1092, Faculté de Médecine, Limoges, France.|CHU Limoges, Laboratoire de Bactériologie-Virologie-Hygiène, Limoges, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Barraud
authors.affiliation: INSERM, U1092, Limoges, France marie-cecile.ploy@unilim.fr.|Université de Limoges, UMR-S1092, Faculté de Médecine, Limoges, France.|CHU Limoges, Laboratoire de Bactériologie-Virologie-Hygiène, Limoges, France.
authors.forename: Marie-Cécile
authors.initials: MC
authors.lastname: Ploy
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D002701:Chloramphenicol / Q000494:pharmacology*; D021902:Enterobacter aerogenes / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D014644:Genetic Variation; D032023:Integrons / Q000235:genetics*; D012641:Selection, Genetic / Q000235:genetics*; D012762:Shigella dysenteriae / Q000235:genetics / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D002701:Chloramphenicol