title: Intranuclear dynamics of the Nup107-160 complex.
abstract: Nup98 is a glycine-leucine-phenylalanine-glycine (GLFG) repeat-containing nucleoporin that, in addition to nuclear transport, contributes to multiple aspects of gene regulation. Previous studies revealed its dynamic localization within intranuclear structures known as GLFG bodies. Here we show that the mammalian Nup107-160 complex (Y-complex), a major scaffold module of the nuclear pore, together with its partner Elys, colocalizes with Nup98 in GLFG bodies. The frequency and size of GLFG bodies vary among HeLa sublines, and we find that an increased level of Nup98 is associated with the presence of bodies. Recruitment of the Y-complex and Elys into GLFG bodies requires the C-terminal domain of Nup98. During cell division, Y-Nup-containing GLFG bodies are disassembled in mitotic prophase, significantly ahead of nuclear pore disassembly. FRAP studies revealed that, unlike at nuclear pores, the Y-complex shuttles into and out of GLFG bodies. Finally, we show that within the nucleoplasm, a fraction of Nup107, a key component of the Y-complex, displays reduced mobility, suggesting interaction with other nuclear components. Together our data uncover a previously neglected intranuclear pool of the Y-complex that may underscore a yet-uncharacterized function of these nucleoporins inside the nucleus, even in cells that contain no detectable GLFG bodies.
authors.affiliation: Institut Jacques Monod, CNRS UMR7592-Université Paris Diderot, Sorbonne Paris Cité, 75205 Paris, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Morchoisne-Bolhy
authors.affiliation: Institut Jacques Monod, CNRS UMR7592-Université Paris Diderot, Sorbonne Paris Cité, 75205 Paris, France.
authors.forename: Marie-Claude
authors.initials: MC
authors.lastname: Geoffroy
authors.affiliation: Institut Jacques Monod, CNRS UMR7592-Université Paris Diderot, Sorbonne Paris Cité, 75205 Paris, France.
authors.forename: Imène B
authors.initials: IB
authors.lastname: Bouhlel
authors.affiliation: Institut Jacques Monod, CNRS UMR7592-Université Paris Diderot, Sorbonne Paris Cité, 75205 Paris, France Ecole Doctorale Gènes Génomes Cellules, Université Paris Sud, 91405 Orsay, France.
authors.forename: Annabelle
authors.initials: A
authors.lastname: Alves
authors.affiliation: Institut Jacques Monod, CNRS UMR7592-Université Paris Diderot, Sorbonne Paris Cité, 75205 Paris, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Audugé
authors.affiliation: ImagoSeine Imaging Facility, Institut Jacques Monod, 75205 Paris, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Baudin
authors.affiliation: Department of Cell Biology and Biochemistry, Cell and Developmental Biology Graduate Program, Emory University School of Medicine, Atlanta, GA 30322.
authors.forename: Kevin
authors.initials: K
authors.lastname: Van Bortle
authors.affiliation: Department of Cell Biology and Biochemistry, Cell and Developmental Biology Graduate Program, Emory University School of Medicine, Atlanta, GA 30322.
authors.forename: Maureen A
authors.initials: MA
authors.lastname: Powers
authors.affiliation: Institut Jacques Monod, CNRS UMR7592-Université Paris Diderot, Sorbonne Paris Cité, 75205 Paris, France valerie.doye@ijm.fr.
authors.forename: Valérie
authors.initials: V
authors.lastname: Doye
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism* / Q000502:physiology; D004268:DNA-Binding Proteins / Q000378:metabolism; D036681:Fluorescence Recovery After Photobleaching; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis; D028861:Nuclear Pore Complex Proteins / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism
chemical_list: C517876:AHCTF1 protein, human; D004268:DNA-Binding Proteins; C512772:NUP107 protein, human; C440251:NUP160 protein, human; D028861:Nuclear Pore Complex Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors