title: Distribution of Sec24 isoforms to each ER exit site is dynamically regulated in Saccharomyces cerevisiae.
abstract: COPII vesicles are formed at specific subdomains of the ER, termed ER exit sites (ERESs). Depending on the cell type, ERESs number from a few to several hundred per cell. However, whether these ERESs are functionally and compositionally identical at the cellular level remains unclear. Our live cell-imaging analysis in Saccharomyces cerevisiae revealed that the isoforms of cargo-adaptor subunits are unequally distributed to each ERES at steady state, whereas this distribution is altered in response to UPR activation. These results suggest that in S. cerevisiae cargo loading to ERES is dynamically controlled in response to environmental changes.
authors.affiliation: Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Komaba, Meguro-ku, Tokyo 153-8902, Japan.
authors.forename: Hirohiko
authors.initials: H
authors.lastname: Iwasaki
authors.affiliation: Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Komaba, Meguro-ku, Tokyo 153-8902, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Yorimitsu
authors.affiliation: Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Komaba, Meguro-ku, Tokyo 153-8902, Japan. Electronic address: kensato@bio.c.u-tokyo.ac.jp.
authors.forename: Ken
authors.initials: K
authors.lastname: Sato
mesh_terms: D022181:COP-Coated Vesicles / Q000235:genetics / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D020033:Protein Isoforms; C118289:SEC24 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins
keywords: COPII; ER exit site; Endoplasmic reticulum; Lst1; Sec24; UPR