title: Peroxisomal NADP-isocitrate dehydrogenase is required for Arabidopsis stomatal movement.
abstract: Peroxisomes are subcellular organelles characterized by a simple morphological structure but have a complex biochemical machinery involved in signaling processes through molecules such as hydrogen peroxide (H2O2) and nitric oxide (NO). Nicotinamide adenine dinucleotide phosphate (NADPH) is an essential component in cell redox homeostasis, and its regeneration is critical for reductive biosynthesis and detoxification pathways. Plants have several NADPH-generating dehydrogenases, with NADP-isocitrate dehydrogenase (NADP-ICDH) being one of these enzymes. Arabidopsis contains three genes that encode for cytosolic, mitochondrial/chloroplastic, and peroxisomal NADP-ICDH isozymes although the specific function of each of these remains largely unknown. Using two T-DNA insertion lines of the peroxisomal NADP-ICDH designated as picdh-1 and picdh-2, the data show that the peroxisomal NADP-ICDH is involved in stomatal movements, suggesting that peroxisomes are a new element in the signaling network of guard cells.
authors.affiliation: Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Apartado 419, 18080, Granada, Spain.
authors.forename: Marina
authors.initials: M
authors.lastname: Leterrier
authors.affiliation: Group of Biochemistry and Cell Signaling in Nitric Oxide, Department of Biochemistry and Molecular Biology, University of Jaén, Campus "Las Lagunillas", 23071, Jaén, Spain.
authors.forename: Juan B
authors.initials: JB
authors.lastname: Barroso
authors.affiliation: Group of Biochemistry and Cell Signaling in Nitric Oxide, Department of Biochemistry and Molecular Biology, University of Jaén, Campus "Las Lagunillas", 23071, Jaén, Spain.
authors.forename: Raquel
authors.initials: R
authors.lastname: Valderrama
authors.affiliation: Group of Biochemistry and Cell Signaling in Nitric Oxide, Department of Biochemistry and Molecular Biology, University of Jaén, Campus "Las Lagunillas", 23071, Jaén, Spain.
authors.forename: Juan C
authors.initials: JC
authors.lastname: Begara-Morales
authors.affiliation: Group of Biochemistry and Cell Signaling in Nitric Oxide, Department of Biochemistry and Molecular Biology, University of Jaén, Campus "Las Lagunillas", 23071, Jaén, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Sánchez-Calvo
authors.affiliation: Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Apartado 419, 18080, Granada, Spain.
authors.forename: Mounira
authors.initials: M
authors.lastname: Chaki
authors.affiliation: Group of Biochemistry and Cell Signaling in Nitric Oxide, Department of Biochemistry and Molecular Biology, University of Jaén, Campus "Las Lagunillas", 23071, Jaén, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Luque
authors.affiliation: Departamento de Biología Animal, Biología Vegetal y Ecología (Ecología), Facultad de Ciencias Experimentales, Universidad de Jaén, Jaén, Spain.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Viñegla
authors.affiliation: Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Apartado 419, 18080, Granada, Spain.
authors.forename: José M
authors.initials: JM
authors.lastname: Palma
authors.affiliation: Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Apartado 419, 18080, Granada, Spain. javier.corpas@eez.csic.es.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Corpas
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000648:ultrastructure; D029681:Arabidopsis Proteins / Q000502:physiology*; D007521:Isocitrate Dehydrogenase / Q000502:physiology*; D020675:Peroxisomes / Q000201:enzymology*; D054046:Plant Stomata / Q000201:enzymology* / Q000502:physiology
chemical_list: D029681:Arabidopsis Proteins; D007521:Isocitrate Dehydrogenase; C588248:ICDH protein, Arabidopsis
keywords: Guard cells; Hydrogen peroxide; NADP-isocitrate dehydrogenase; Nitric oxide; Peroxisomes; Stomata