title: Centella asiatica and Its Fractions Reduces Lipid Peroxidation Induced by Quinolinic Acid and Sodium Nitroprusside in Rat Brain Regions.
abstract: Oxidative stress has been implicated in several pathologies including neurological disorders. Centella asiatica is a popular medicinal plant which has long been used to treat neurological disturbances in Ayurvedic medicine. In the present study, we quantified of compounds by high performance liquid chromatography (HPLC) and examined the phenolic content of infusion, ethyl acetate, n-butanolic and dichloromethane fractions. Furthermore, we analyzed the ability of the extracts from C. asiatica to scavenge the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) radical as well as total antioxidant activity through the reduction of molybdenum (VI) (Mo(6+)) to molybdenum (V) (Mo(5+)). Finally, we examined the antioxidant effect of extracts against oxidant agents, quinolinic acid (QA) and sodium nitroprusside (SNP), on homogenates of different brain regions (cerebral cortex, striatum and hippocampus). The HPLC analysis revealed that flavonoids, triterpene glycoside, tannins, phenolic acids were present in the extracts of C. asiatica and also the phenolic content assay demonstrated that ethyl acetate fraction is rich in these compounds. Besides, the ethyl acetate fraction presented the highest antioxidant effect by decreasing the lipid peroxidation in brain regions induced by QA. On the other hand, when the pro-oxidant agent was SNP, the potency of infusion, ethyl acetate and dichloromethane fractions was equivalent. Ethyl acetate fraction from C. asiatica also protected against thiol oxidation induced by SNP and QA. Thus, the therapeutic potential of C. asiatica in neurological diseases could be associated to its antioxidant activity.
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas, Bioquímica Toxicológica, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Naiani Ferreira
authors.initials: NF
authors.lastname: Marques
authors.forename: Sílvio Terra
authors.initials: ST
authors.lastname: Stefanello
authors.forename: Amanda L F
authors.initials: AL
authors.lastname: Froeder
authors.forename: Alcindo
authors.initials: A
authors.lastname: Busanello
authors.forename: Aline Augusti
authors.initials: AA
authors.lastname: Boligon
authors.forename: Margareth Linde
authors.initials: ML
authors.lastname: Athayde
authors.forename: Félix A A
authors.initials: FA
authors.lastname: Soares
authors.forename: Roselei
authors.initials: R
authors.lastname: Fachinetto
mesh_terms: D000085:Acetates; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001923:Brain Chemistry / Q000187:drug effects*; D028041:Centella / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008982:Molybdenum / Q000737:chemistry; D009599:Nitroprusside / Q000494:pharmacology*; D016877:Oxidants / Q000494:pharmacology*; D010084:Oxidation-Reduction; D010936:Plant Extracts; D017378:Quinolinic Acid / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012997:Solvents; D014315:Triterpenes / Q000494:pharmacology*
chemical_list: D000085:Acetates; D000975:Antioxidants; C526146:Centella asiatica extract; D016166:Free Radical Scavengers; D016877:Oxidants; D010936:Plant Extracts; D012997:Solvents; D014315:Triterpenes; D009599:Nitroprusside; C007650:ethyl acetate; D008982:Molybdenum; D017378:Quinolinic Acid