title: Sentrin/small ubiquitin-like modifier-specific protease 5 protects oral cancer cells from oxidative stress-induced apoptosis.
abstract: The aim of the present study was to investigate the role of sentrin/small ubiquitin-like modifier (SUMO)-specific protease 5 (SENP5) in oral squamous cell carcinoma (OSCC), as the overexpression of SENP5 has been observed in 31 OSCC tissue specimens. CAL-27 OSCC cells were used for in vitro measurements. The distribution of SENP5 was visualized using immunohistochemistry and H2O2-induced oxidative stress, and the effects of SENP-small interfering RNA on SENP5 were analyzed via western blotting. The apoptotic rates of the CAL-27 cells during oxidative stress and SENP5 silencing were estimated using flow-cytometry, and the mitochondrial structures were analyzed using a mitochondria tracker. The SENP5 protein was localized in the nuclei and cytosols of the CAL-27 cells, and incubation with 100 µm H2O2 for >1 h led to its stabilization. Incubation with H2O2 alone had no effect on the CAL-27 cells, however, a combination of H2O2 and SENP5 silencing led to enhanced apoptotic rates (P<0.001). Analysis of the mitochondrial structures revealed that H2O2 alone enhanced mitochondrial network formation, whereas the combination of H2O2 and SENP5 silencing led to mitochondrial fragmentation in the CAL-27 cells. The overexpression of SENP5 partly localized in the cytosol of the OSCC cells. Mild oxidative stress stabilized the SENP5 protein in the CAL-27 cells, and only the combination of SENP5 silencing and H2O2 application led to mitochondria fragmentation and a significant increase in cell apoptosis. Therefore, SENP5 protected the OSCC cells from oxidative stress-induced apoptosis.
authors.affiliation: Department of Stomatology, Zhongshan Hospital of Fudan University, Shanghai 200032, P.R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Department of Stomatology, Zhongshan Hospital of Fudan University, Shanghai 200032, P.R. China.
authors.forename: Xuehua
authors.initials: X
authors.lastname: Guo
authors.affiliation: Department of Stomatology, Zhongshan Hospital of Fudan University, Shanghai 200032, P.R. China.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Gong
authors.affiliation: Department of Stomatology, Zhongshan Hospital of Fudan University, Shanghai 200032, P.R. China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Ding
authors.affiliation: Department of Stomatology, Zhongshan Hospital of Fudan University, Shanghai 200032, P.R. China.
authors.forename: Youcheng
authors.initials: Y
authors.lastname: Yu
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D009055:Mouth / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D009062:Mouth Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D018384:Oxidative Stress; D010447:Peptide Hydrolases / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering
chemical_list: D034741:RNA, Small Interfering; D006861:Hydrogen Peroxide; D010447:Peptide Hydrolases; C512579:Senp5 protein, human