title: Protective effect of resveratrol on lens epithelial cell apoptosis in diabetic cataract rat.
abstract: OBJECTIVE
  To study the protective effect of resveratrol on lens epithelial cell apoptosis in diabetic cataract rat.
  
  
  METHODS
  A total of 84 Wistar rats were divided into 4 groups: 12 in Group A (control group), 24 in Group B (diabetic cataract group), 24 in Group C (therapeutic-dose of resveratrol group) and 24 in Group D (low-dose of resveratrol group). Rats in Group B-D were given with 60 mg/kg streptozotocin through intraperitoneal injection. Rats in Group C were given with 100 mg/kg resveratrol and rats in Group D were given with 20 mg/kg resveratrol. The caspase-3 expression levels and apoptosis ratios of LEC among each group were observed; the degrees of lens opacity in Group B-D after 12 weeks were compared.
  
  
  RESULTS
  There were significant differences in caspase-3 expression levels, apoptosis ratios of LEC among groups at 4 w, 8 w and 12 w (P<0.05). After 12 weeks, in Group B the degree of lens opacity was as follow: 0 (0.00%) in grade I, 3 (37.50%) in grade II, 2 (25.00%)in grade III, 2 (25.00%)grade IV, and 1 (12.50%) in grade V; in Group C: 2 (25.00%)in grade I, 4 (50.00%) in grade II, 2 (25.00%)in grade III, 0 (0.00%)grade IV, and 0 (0.00 %) in grade V; in Group D: 1 (12.50%)in grade I, 4 (50.00%) in grade II, 2 (25.00%) in grade III, 1 (12.50%) grade IV, and 0 (0.00%) in grade V. The difference among Group B-D was statistically significant (P<0.05).
  
  
  CONCLUSIONS
  Resveratrol has protective effect on lens epithelial cell apoptosis in diabetic cataract rat, and the effect is relative to its dose.
authors.affiliation: Center Hospital Affiliated to Zhengzhou University, Zhumadian 463000, China. Electronic address: wanghongmin21@126.com.
authors.forename: Hong-Min
authors.initials: HM
authors.lastname: Wang
authors.affiliation: Eye Hospital Affiliated to Wenzhou Medical College, Wenzhou 325000, China.
authors.forename: Guo-Xing
authors.initials: GX
authors.lastname: Li
authors.affiliation: Center Hospital Affiliated to Zhengzhou University, Zhumadian 463000, China.
authors.forename: Han-Song
authors.initials: HS
authors.lastname: Zheng
authors.affiliation: Center Hospital Affiliated to Zhengzhou University, Zhumadian 463000, China.
authors.forename: Xue-Zhi
authors.initials: XZ
authors.lastname: Wu
keywords: Cell apoptosis; Diabetic cataract; Lens epithelial ce11; Resveratrol