title: Primary Human Lung Pericytes Support and Stabilize In Vitro Perfusable Microvessels.
abstract: The formation of blood vessels is a complex tissue-specific process that plays a pivotal role during developmental processes, in wound healing, cancer progression, fibrosis, and other pathologies. To study vasculogenesis and vascular remodeling in the context of the lung, we developed an in vitro microvascular model that closely mimics the human lung microvasculature in terms of three-dimensional architecture, accessibility, functionality, and cell types. Human pericytes from the distal airway were isolated and characterized using flow cytometry. To assess their role in the generation of normal microvessels, lung pericytes were mixed in fibrin gel and seeded into well-defined microcompartments together with primary endothelial cells (human umbilical cord vein endothelial cells). Patent microvessels covering an area of 3.1 mm(2) formed within 3-5 days and were stable for up to 14 days. Soluble signals from the lung pericytes were necessary to establish perfusability, and pericytes migrated toward endothelial microvessels. Cell-cell communication in the form of adherens and tight junctions, as well as secretion of basement membrane were confirmed using transmission electron microscopy and immunocytochemistry on chip. Direct coculture of pericytes with endothelial cells decreased the microvascular permeability by one order of magnitude from 17.8×10(-6) to 2.0×10(-6) cm/s and led to vessels with significantly smaller and less variable diameter. Upon phenylephrine administration, vasoconstriction was observed in microvessels lined with pericytes, but not in endothelial microvessels only. Perfusable microvessels were also generated with human lung microvascular endothelial cells and lung pericytes. Human lung pericytes were thus shown to have a prominent influence on microvascular morphology, permeability, vasoconstriction, and long-term stability in an in vitro microvascular system. This biomimetic platform opens new possibilities to test functions and interactions of patient-derived cells in a physiologically relevant microvascular setting.
authors.affiliation: 1 Lung Regeneration Technologies, ARTORG Center, University of Bern , Bern, Switzerland .|2 Division of Pulmonary Medicine, University Hospital of Bern , Bern, Switzerland .
authors.forename: Colette A
authors.initials: CA
authors.lastname: Bichsel
authors.affiliation: 3 Division of Thoracic Surgery, University Hospital of Bern , Bern, Switzerland .|4 Department of Clinical Research, University of Bern , Bern, Switzerland .
authors.forename: Sean R R
authors.initials: SR
authors.lastname: Hall
authors.affiliation: 3 Division of Thoracic Surgery, University Hospital of Bern , Bern, Switzerland .|4 Department of Clinical Research, University of Bern , Bern, Switzerland .
authors.forename: Ralph A
authors.initials: RA
authors.lastname: Schmid
authors.affiliation: 1 Lung Regeneration Technologies, ARTORG Center, University of Bern , Bern, Switzerland .|2 Division of Pulmonary Medicine, University Hospital of Bern , Bern, Switzerland .|3 Division of Thoracic Surgery, University Hospital of Bern , Bern, Switzerland .
authors.forename: Olivier T
authors.initials: OT
authors.lastname: Guenat
authors.affiliation: 2 Division of Pulmonary Medicine, University Hospital of Bern , Bern, Switzerland .|4 Department of Clinical Research, University of Bern , Bern, Switzerland .
authors.forename: Thomas
authors.initials: T
authors.lastname: Geiser
mesh_terms: D002478:Cells, Cultured; D018920:Coculture Techniques; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D008168:Lung / Q000166:cytology / Q000378:metabolism*; D055806:Microvessels / Q000166:cytology / Q000378:metabolism*; D008955:Models, Cardiovascular; D018919:Neovascularization, Physiologic; D020286:Pericytes / Q000166:cytology / Q000378:metabolism*; D066253:Vascular Remodeling