title: Mobility of calcium channels in the presynaptic membrane.
abstract: Unravelling principles underlying neurotransmitter release are key to understand neural signaling. Here, we describe how surface mobility of voltage-dependent calcium channels (VDCCs) modulates release probabilities (P(r)) of synaptic vesicles (SVs). Coupling distances of <10 to >100 nm have been reported for SVs and VDCCs in different synapses. Tracking individual VDCCs revealed that within hippocampal synapses, ∼60% of VDCCs are mobile while confined to presynaptic membrane compartments. Intracellular Ca(2+) chelation decreased VDCC mobility. Increasing VDCC surface populations by co-expression of the α2δ1 subunit did not alter channel mobility but led to enlarged active zones (AZs) rather than higher channel densities. VDCCs thus scale presynaptic scaffolds to maintain local mobility. We propose that dynamic coupling based on mobile VDCCs supports calcium domain cooperativity and tunes neurotransmitter release by equalizing Pr for docked SVs within AZs.
authors.affiliation: Molecular Physiology Group, Leibniz Institute for Neurobiology, D-39118 Magdeburg, Germany.
authors.forename: Romy
authors.initials: R
authors.lastname: Schneider
authors.affiliation: University of Bordeaux, F-33000 Bordeaux, France; CNRS, Interdisciplinary Institute for Neuroscience, UMR 5297, F-33000 Bordeaux, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Hosy
authors.affiliation: Harvard University, Department Molecular and Cellular Biology, Cambridge, MA 02138, USA.
authors.forename: Johannes
authors.initials: J
authors.lastname: Kohl
authors.affiliation: Molecular Physiology Group, Leibniz Institute for Neurobiology, D-39118 Magdeburg, Germany; Presynaptic Plasticity Group, Leibniz Institute for Neurobiology, D-39118 Magdeburg, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Klueva
authors.affiliation: University of Bordeaux, F-33000 Bordeaux, France; CNRS, Interdisciplinary Institute for Neuroscience, UMR 5297, F-33000 Bordeaux, France; Bordeaux Imaging Center, UMS 3420 CNRS, US4 INSERM, University of Bordeaux, F-33000 Bordeaux, France.
authors.forename: Daniel
authors.initials: D
authors.lastname: Choquet
authors.affiliation: Department Neurochemistry, Leibniz Institute for Neurobiology, D-39118 Magdeburg, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Thomas
authors.affiliation: Otto-von-Guericke-University Magdeburg, Systemverfahrenstechnik, Universitätsplatz 2, D-39106 Magdeburg, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Voigt
authors.affiliation: Molecular Physiology Group, Leibniz Institute for Neurobiology, D-39118 Magdeburg, Germany. Electronic address: martin.heine@lin-magdeburg.de.
authors.forename: Martin
authors.initials: M
authors.lastname: Heine
mesh_terms: D000200:Action Potentials / Q000187:drug effects / Q000235:genetics; D000818:Animals; D019256:Cadmium Chloride / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000494:pharmacology; D015220:Calcium Channels / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004622:Embryo, Mammalian; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects / Q000235:genetics; D006624:Hippocampus / Q000166:cytology; D008954:Models, Biological; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000166:cytology*; D011189:Potassium Chloride / Q000494:pharmacology; D017729:Presynaptic Terminals / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D021381:Protein Transport / Q000235:genetics / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013572:Synaptic Vesicles / Q000187:drug effects / Q000378:metabolism; D050863:Synaptotagmin I / Q000378:metabolism
chemical_list: C113688:Bsn protein, rat; D002121:Calcium Channel Blockers; D015220:Calcium Channels; D009419:Nerve Tissue Proteins; D050863:Synaptotagmin I; D011189:Potassium Chloride; D019256:Cadmium Chloride; D002118:Calcium