title: [The method and result analyses of pathogenic bacteria culture on chronic periprosthetic joint infection after total knee arthroplasty and total hip arthroplasty].
abstract: OBJECTIVE
  To analyze the results of pathogenic bacteria culture on chronic periprosthetic joint infection after total knee arthroplasty (TKA) and total hip arthroplasty (THA).
  
  
  METHODS
  The medical data of 23 patients with chronic periprosthetic joint infection after TKA or THA from September 2010 to March 2014 were reviewed. Fifteen cases of TKA and 8 cases of THA were included in this study. There were 12 male and 11 female patients with the mean age of 62 years (range from 32 to 79 years), and among them 9 patients with sinus. All patients discontinued antibiotic therapy for a minimum of 2 weeks before arthrocentesis, taking pathogenic bacteria culture and antimicrobial susceptibility test by using synovial fluid taken preoperatively and intraoperatively of revision. Common pathogenic bacteria culture and pathological biopsy were taken on tissues intraoperatively of revision. Culture-negative specimens were prolonged the period of incubation for 2 weeks.
  
  
  RESULTS
  The overall culture-positive rate of all 23 patients for 1 week before revision was 30.4% (7/23), and the positive rate of culture-negative samples which prolonged for 2 weeks was 39.1% (9/23). The overall culture-positive rate of patients for 1 week intraoperatively of revision was 60.9% (14/23), and the positive rate of culture-negative samples which prolonged for 2 weeks was 82.6% (19/23). The incubation results of 7 cases (30.4%) preoperatively conformed to that of intraoperation.
  
  
  CONCLUSIONS
  The culture-positive rate of pathogenic bacteria culture can be increased evidently by discontinuing antimicrobial therapy for a minimum of 2 weeks prior to the definite diagnosis.
authors.affiliation: Department of Orthopaedic Surgery, First Teaching Hospital of Xinjiang Medical University, Urumqi 830054, China.
authors.forename: Baochao
authors.initials: B
authors.lastname: Ji
authors.forename: Enjie
authors.initials: E
authors.lastname: Xu
authors.affiliation: Email: xjbone@21cn.com.
authors.forename: Li
authors.initials: L
authors.lastname: Cao
authors.forename: Desheng
authors.initials: D
authors.lastname: Yang
authors.forename: Boyong
authors.initials: B
authors.lastname: Xu
authors.forename: Wentao
authors.initials: W
authors.lastname: Guo
authors.forename: Rehei
authors.initials: R
authors.lastname: Aili
mesh_terms: D000328:Adult; D000368:Aged; D019644:Arthroplasty, Replacement, Hip; D019645:Arthroplasty, Replacement, Knee; D001419:Bacteria / Q000302:isolation & purification*; D002908:Chronic Disease; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016459:Prosthesis-Related Infections; D012086:Reoperation