title: Chemicals from Agave sisalana biomass: isolation and identification.
abstract: Agave sisalana (sisal) is known worldwide as a source of hard fibers, and Brazil is the largest producer of sisal. Nonetheless, the process of removing the fibers of the sisal leaf generates 95% waste. In this study, we applied chemical sequential steps (hydrothermal extraction, precipitation, liquid-liquid extraction, crystallization, SiO2 and Sephadex LH 20 column chromatography) to obtain pectin, mannitol, succinic acid, kaempferol and a mixture of saponins as raw chemicals from sisal biomass. The structural identification of these compounds was performed though spectrometric methods, such as Infrared (IR), Ultraviolet (UV), Mass spectrometry (MS) and Nuclear magnetic resonance (NMR). All the sisal chemicals found in this work are used by both the chemical and pharmaceutical industries as excipients or active principles in products.
authors.affiliation: Laboratório de Fitoquímica, Departamento de Saúde, Universidade Estadual de Feira de Santana, Feira de Santana, Bahia 44031-460, Brazil. jennerdgs@yahoo.com.br.
authors.forename: Jener David Gonçalves
authors.initials: JD
authors.lastname: Santos
authors.affiliation: LCQUI-CCT, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Rio de Janeiro 28013-600, Brazil. curcino@uenf.br.
authors.forename: Ivo Jose Curcino
authors.initials: IJ
authors.lastname: Vieira
authors.affiliation: LCQUI-CCT, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Rio de Janeiro 28013-600, Brazil. rbraz@ufrrj.br.|LCQUI-CCT, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes-RJ/DEQUIM-ICE, Universidade Federal Rural do Rio de Janeiro, Seropédica, Rio de Janeiro 23894-374, Brazil. rbraz@ufrrj.br.
authors.forename: Raimundo
authors.initials: R
authors.lastname: Braz-Filho
authors.affiliation: Laboratório de Fitoquímica, Departamento de Saúde, Universidade Estadual de Feira de Santana, Feira de Santana, Bahia 44031-460, Brazil. branco@uefs.br.
authors.forename: Alexsandro
authors.initials: A
authors.lastname: Branco
mesh_terms: D027882:Agave / Q000737:chemistry*; D018533:Biomass; D011232:Chemical Precipitation; D002850:Chromatography, Gel; D003460:Crystallization; D044949:Kaempferols / Q000737:chemistry / Q000302:isolation & purification; D059625:Liquid-Liquid Extraction; D008353:Mannitol / Q000737:chemistry / Q000302:isolation & purification; D010368:Pectins / Q000737:chemistry / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification*; D018515:Plant Leaves / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification; D019802:Succinic Acid / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D044949:Kaempferols; D010936:Plant Extracts; D012503:Saponins; D008353:Mannitol; C006552:kaempferol; D010368:Pectins; D019802:Succinic Acid