title: Isolation of Intact Mitochondria from the Model Plant Species Arabidopsis thaliana and Oryza sativa.
abstract: The study of mitochondrial composition, biogenesis, and metabolism requires the isolation of intact functional mitochondria, often in relatively large quantities sufficient for downstream biochemical and proteomic analysis. Here we describe techniques optimized for the isolation of functional mitochondria from a variety of tissues from the model monocot and dicot species Arabidopsis (Arabidopsis thaliana) and rice (Oryza sativa).
authors.affiliation: Australian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009, Australia, monika.murcha@uwa.edu.au.
authors.forename: Monika W
authors.initials: MW
authors.lastname: Murcha
authors.forename: James
authors.initials: J
authors.lastname: Whelan
mesh_terms: D017360:Arabidopsis / Q000166:cytology* / Q000254:growth & development; D002458:Cell Fractionation / Q000379:methods*; D008928:Mitochondria / Q000737:chemistry; D012275:Oryza / Q000166:cytology* / Q000196:embryology / Q000254:growth & development