title: Development of a graded index microlens based fiber optical trap and its characterization using principal component analysis.
abstract: We demonstrate a miniaturized single beam fiber optical trapping probe based on a high numerical aperture graded index (GRIN) micro-objective lens. This enables optical trapping at a distance of 200μm from the probe tip. The fiber trapping probe is characterized experimentally using power spectral density analysis and an original approach based on principal component analysis for accurate particle tracking. Its use for biomedical microscopy is demonstrated through optically mediated immunological synapse formation.
authors.affiliation: SUPA, School of Physics and Astronomy, University of St Andrews, St Andrews, KY16 9SS, UK ; School of Biology, University of St Andrews, St Andrews, KY16 9TF, UK.
authors.forename: J
authors.initials: J
authors.lastname: Nylk
authors.affiliation: SUPA, School of Physics and Astronomy, University of St Andrews, St Andrews, KY16 9SS, UK.
authors.forename: M V G
authors.initials: MV
authors.lastname: Kristensen
authors.affiliation: SUPA, School of Physics and Astronomy, University of St Andrews, St Andrews, KY16 9SS, UK.
authors.forename: M
authors.initials: M
authors.lastname: Mazilu
authors.affiliation: SUPA, School of Physics and Astronomy, University of St Andrews, St Andrews, KY16 9SS, UK ; School of Biology, University of St Andrews, St Andrews, KY16 9TF, UK.
authors.forename: A K
authors.initials: AK
authors.lastname: Thayil
authors.affiliation: SUPA, School of Physics and Astronomy, University of St Andrews, St Andrews, KY16 9SS, UK ; School of Biology, University of St Andrews, St Andrews, KY16 9TF, UK.
authors.forename: C A
authors.initials: CA
authors.lastname: Mitchell
authors.affiliation: School of Biology, University of St Andrews, St Andrews, KY16 9TF, UK.
authors.forename: E C
authors.initials: EC
authors.lastname: Campbell
authors.affiliation: School of Medicine, University of St Andrews, St Andrews, KY16 9TF, UK.
authors.forename: S J
authors.initials: SJ
authors.lastname: Powis
authors.affiliation: School of Biology, University of St Andrews, St Andrews, KY16 9TF, UK.
authors.forename: F J
authors.initials: FJ
authors.lastname: Gunn-Moore
authors.affiliation: SUPA, School of Physics and Astronomy, University of St Andrews, St Andrews, KY16 9SS, UK.
authors.forename: K
authors.initials: K
authors.lastname: Dholakia
keywords: (060.2310) Fiber optics; (110.2760) Gradient-index lenses; (170.0170) Medical optics and biotechnology; (170.3880) Medical and biological imaging; (170.4520) Optical confinement and manipulation; (180.0180) Microscopy; (180.2520) Fluorescence microscopy; (350.4855) Optical tweezers or optical manipulation