title: Prophylactic effects of asiaticoside-based standardized extract of Centella asiatica (L.) Urban leaves on experimental migraine: Involvement of 5HT1A/1B receptors.
abstract: The present study aimed at evaluation of prophylactic efficacy and possible mechanisms of asiaticoside (AS) based standardized extract of Centella asiatica (L.) Urban leaves (INDCA) in animal models of migraine. The effects of oral and intranasal (i.n.) pretreatment of INDCA (acute and 7-days subacute) were evaluated against nitroglycerine (NTG, 10 mg·kg(-1), i.p.) and bradykinin (BK, 10 μg, intra-arterial) induced hyperalgesia in rats. Tail flick latencies (from 0 to 240 min) post-NTG treatment and the number of vocalizations post-BK treatment were recorded as a measure of hyperalgesia. Separate groups of rats for negative (Normal) and positive (sumatriptan, 42 mg·kg(-1), s.c.) controls were included. The interaction of INDCA with selective 5-HT1A, 5-HT1B, and 5-HT1D receptor antagonists (NAN-190, Isamoltane hemifumarate, and BRL-15572 respectively) against NTG-induced hyperalgesia was also evaluated. Acute and sub-acute pre-treatment of INDCA [10 and 30 mg·kg(-1) (oral) and 100 μg/rat (i.n.) showed significant anti-nociception activity, and reversal of the NTG-induced hyperalgesia and brain 5-HT concentration decline. Oral pre-treatment with INDCA (30 mg·kg(-1), 7 d) showed significant reduction in the number of vocalization. The anti-nociceptive effects of INDCA were blocked by 5-HT1A and 5-HT1B but not 5-HT1D receptor antagonists. In conclusion, INDCA demonstrated promising anti-nociceptive effects in animal models of migraine, probably through 5-HT1A/1B medicated action.
authors.affiliation: Department of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University, Erandwane, Pune-411038, India.
authors.forename: Vijeta
authors.initials: V
authors.lastname: Bobade
authors.affiliation: Department of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University, Erandwane, Pune-411038, India. Electronic address: sbodhindus@gmail.com.
authors.forename: Subhash L
authors.initials: SL
authors.lastname: Bodhankar
authors.affiliation: Department of Pharmacology, Sinhgad Institute of Pharmacy, Narhe, Pune 411041, India.
authors.forename: Urmila
authors.initials: U
authors.lastname: Aswar
authors.affiliation: Indus Biotech Private Limited, 1, Rahul residency, Kondhwa, Pune-411026, India.
authors.forename: Mohan
authors.initials: M
authors.lastname: Vishwaraman
authors.affiliation: Indus Biotech Private Limited, 1, Rahul residency, Kondhwa, Pune-411026, India.
authors.forename: Prasad
authors.initials: P
authors.lastname: Thakurdesai
mesh_terms: D000281:Administration, Intranasal; D000284:Administration, Oral; D000818:Animals; D001920:Bradykinin; D028041:Centella; D005260:Female; D006930:Hyperalgesia / Q000139:chemically induced / Q000517:prevention & control; D008297:Male; D008881:Migraine Disorders / Q000139:chemically induced / Q000517:prevention & control*; D023421:Models, Animal; D005996:Nitroglycerin; D059225:Nociception / Q000187:drug effects; D010936:Plant Extracts; D018515:Plant Leaves / Q000737:chemistry; D065129:Pre-Exposure Prophylaxis; D051381:Rats; D017208:Rats, Wistar; D011930:Reaction Time; D044263:Receptors, Serotonin, 5-HT1 / Q000187:drug effects*; D058829:Serotonin 5-HT1 Receptor Antagonists / Q000378:metabolism; D013623:Tail / Q000502:physiology; D014315:Triterpenes / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: C526146:Centella asiatica extract; D010936:Plant Extracts; D044263:Receptors, Serotonin, 5-HT1; D058829:Serotonin 5-HT1 Receptor Antagonists; D014315:Triterpenes; D005996:Nitroglycerin; C004446:asiaticoside; D001920:Bradykinin
keywords: 5HT1A and 5HT1B receptors; Anti-nociception; Centella asiatica (L.) Urban; Experimental migraine; Serotonin