title: Transcriptional profiling of mouse B cell terminal differentiation defines a signature for antibody-secreting plasma cells.
abstract: When B cells encounter an antigen, they alter their physiological state and anatomical localization and initiate a differentiation process that ultimately produces antibody-secreting cells (ASCs). We have defined the transcriptomes of many mature B cell populations and stages of plasma cell differentiation in mice. We provide a molecular signature of ASCs that highlights the stark transcriptional divide between B cells and plasma cells and enables the demarcation of ASCs on the basis of location and maturity. Changes in gene expression correlated with cell-division history and the acquisition of permissive histone modifications, and they included many regulators that had not been previously implicated in B cell differentiation. These findings both highlight and expand the core program that guides B cell terminal differentiation and the production of antibodies.
authors.affiliation: 1] The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia. [2] Department of Computing and Information Systems, The University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
authors.affiliation: 1] The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia. [2] Department of Medical Biology, The University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liao
authors.affiliation: 1] The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia. [2] Department of Medical Biology, The University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Simon N
authors.initials: SN
authors.lastname: Willis
authors.affiliation: 1] The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia. [2] Department of Medical Biology, The University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Nadine
authors.initials: N
authors.lastname: Taubenheim
authors.affiliation: 1] The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia. [2] Department of Pathology, The University of Melbourne, Parkville, Victoria, Australia. [3] Department of Microbiology &Immunology, The University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Michael
authors.initials: M
authors.lastname: Inouye
authors.affiliation: 1] The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia. [2] Department of Medical Biology, The University of Melbourne, Parkville, Victoria, Australia.
authors.forename: David M
authors.initials: DM
authors.lastname: Tarlinton
authors.affiliation: 1] The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia. [2] Department of Mathematics and Statistics, The University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Gordon K
authors.initials: GK
authors.lastname: Smyth
authors.affiliation: 1] The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia. [2] Department of Medical Biology, The University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Philip D
authors.initials: PD
authors.lastname: Hodgkin
authors.affiliation: 1] The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia. [2] Department of Medical Biology, The University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Stephen L
authors.initials: SL
authors.lastname: Nutt
authors.affiliation: 1] The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia. [2] Department of Medical Biology, The University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Lynn M
authors.initials: LM
authors.lastname: Corcoran
mesh_terms: D000818:Animals; D053301:B-Cell Maturation Antigen / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics*; D002455:Cell Division / Q000235:genetics; D002465:Cell Movement / Q000235:genetics; D002478:Cells, Cultured; D020869:Gene Expression Profiling; D042421:Histone Code / Q000235:genetics; D008213:Lymphocyte Activation / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010950:Plasma Cells / Q000166:cytology* / Q000276:immunology*; D000074462:Positive Regulatory Domain I-Binding Factor 1; D012313:RNA / Q000032:analysis; D050826:Suppressor of Cytokine Signaling Proteins / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D059467:Transcriptome
chemical_list: D053301:B-Cell Maturation Antigen; C513258:Prdm1 protein, mouse; C491409:Socs2 protein, mouse; D050826:Suppressor of Cytokine Signaling Proteins; D014157:Transcription Factors; D012313:RNA; D000074462:Positive Regulatory Domain I-Binding Factor 1