title: [Effect of resveratrol on constrictions of human intrapulmonary arteries ex vivo].
abstract: OBJECTIVE
  To determine the effect of resveratrol on constrictions of isolated human intrapulmonary arteries and its mechanisms.
  
  
  METHODS
  Intrapulmonary arteries (1-1.5 mm in diameter) were dissected and cut into rings (1.8-2.0 mm in length) under microscope, and were then mounted in a Multi Myograph system. The rings were stimulated with 100 nmol/L U46619, 30 nmol/L endothelin-1, or 60 mmol/L KCl to produce sustained contraction of the intrapulmonary arteries, after which resveratrol was applied cumulatively. Endothelium denudation, L-NAME and indomethecin were used to investigate the effect of resveratrol on constrictions of the isolated arteries, suing DMSO as the control.
  
  
  RESULTS
  Resveratrol induced concentration-dependent relaxations in endothelium-intact rings that contracted in response to stimulations with U46619, ET-1 and KCl, with pD2 of 3.82±0.20, 3.84±0.57, and 3.68±0.27, Emax of (99.58±0.83)%, 100%, and (99.65±0.98)%, respectively. Treatment of the arterial rings with the eNOS inhibitor L-NAME, but not with indomethecin or endothelium denudation, obviously affected the relaxant effects of resveratrol.
  
  
  CONCLUSIONS
  Resveratrol can concentration-dependently produce relaxant effect on human intrapulmonary arteries independent of the endothelium possibly by promoting synthesis and release of NO.
authors.affiliation: 1Southern Medical University, Guangzhou 510515, China; 3Department of Anesthesiology, Guangdong Academy of Medical Sciences, Guangdong General Hospital, Guangzhou 510080, China. E-mail: smuwangzhaojun@163.com.
authors.forename: Zhao-Jun
authors.initials: ZJ
authors.lastname: Wang
authors.forename: Chun-Yu
authors.initials: CY
authors.lastname: Deng
authors.forename: Su-Juan
authors.initials: SJ
authors.lastname: Kuang
authors.forename: Li-Dan
authors.initials: LD
authors.lastname: Nong
authors.forename: Guang-Yan
authors.initials: GY
authors.lastname: Zhang
authors.forename: Jue
authors.initials: J
authors.lastname: Ma
authors.forename: Jian-Xiu
authors.initials: JX
authors.lastname: Cui
mesh_terms: D019796:15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D011651:Pulmonary Artery / Q000187:drug effects*; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D014661:Vasoconstriction / Q000187:drug effects*
chemical_list: D013267:Stilbenes; D019796:15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid; D000077185:Resveratrol