title: Design of a heterosubtypic epitope-based peptide vaccine fused with hemokinin-1 against influenza viruses.
abstract: Influenza viruses continue to emerge and re-emerge, posing new threats for public health. Control and treatment of influenza depends mainly on vaccination and chemoprophylaxis with approved antiviral drugs. Identification of specific epitopes derived from influenza viruses has significantly advanced the development of epitope-based vaccines. Here, we explore the idea of using HLA binding data to design an epitope-based vaccine that can elicit heterosubtypic T-cell responses against circulating H7N9, H5N1, and H9N2 subtypes. The hemokinin-1 (HK-1) peptide sequence was used to induce immune responses against the influenza viruses. Five conserved high score cytotoxic T lymphocyte (CTL) epitopes restricted to HLA-A*0201-binding peptides within the hemagglutinin (HA) protein of the viruses were chosen, and two HA CTL/HK-1 chimera protein models designed. Using in silico analysis, which involves interferon epitope scanning, protein structure prediction, antigenic epitope determination, and model quality evaluation, chimeric proteins were designed. The applicability of one of these proteins as a heterosubtypic epitopebased vaccine candidate was analyzed.
authors.affiliation: Razi Vaccine & Serum Research Institute, Karaj, 31975, Iran, s.shahsavandi@rvsri.ac.ir.
authors.forename: Shahla
authors.initials: S
authors.lastname: Shahsavandi
authors.forename: Mohammad Majid
authors.initials: MM
authors.lastname: Ebrahimi
authors.forename: Kaveh
authors.initials: K
authors.lastname: Sadeghi
authors.forename: Homayoon
authors.initials: H
authors.lastname: Mahravani
mesh_terms: D000276:Adjuvants, Immunologic / Q000235:genetics / Q000378:metabolism*; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D015789:HLA-A2 Antigen / Q000378:metabolism; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000276:immunology / Q000378:metabolism; D006801:Humans; D009980:Influenza A virus / Q000235:genetics / Q000276:immunology*; D007252:Influenza Vaccines / Q000235:genetics / Q000302:isolation & purification*; D008958:Models, Molecular; D011485:Protein Binding; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D015320:Tachykinins / Q000235:genetics / Q000378:metabolism*; D022223:Vaccines, Subunit / Q000235:genetics / Q000302:isolation & purification*; D014614:Vaccines, Synthetic / Q000235:genetics / Q000302:isolation & purification
chemical_list: D000276:Adjuvants, Immunologic; D000939:Epitopes; C435939:HLA-A*02:01 antigen; D015789:HLA-A2 Antigen; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines; D011993:Recombinant Fusion Proteins; D015320:Tachykinins; D022223:Vaccines, Subunit; D014614:Vaccines, Synthetic; C507968:hemokinin-1