title: Nop17 is a key R2TP factor for the assembly and maturation of box C/D snoRNP complex.
abstract: BACKGROUND
  Box C/D snoRNPs are responsible for rRNA methylation and processing, and are formed by snoRNAs and four conserved proteins, Nop1, Nop56, Nop58 and Snu13. The snoRNP assembly is a stepwise process, involving other protein complexes, among which the R(2)TP and Hsp90 chaperone. Nop17, also known as Pih1, has been shown to be a constituent of the R(2)TP (Rvb1, Rvb2, Tah1, Pih1) and to participate in box C/D snoRNP assembly by its interaction with Nop58. The molecular function of Nop17, however, has not yet been described.
  
  
  RESULTS
  To shed light on the role played by Nop17 in the maturation of snoRNP, here we analyzed the interactions domains of Nop58 - Nop17 - Tah1 and the importance of ATP to the interaction between Nop17 and the ATPase Rvb1/2.
  
  
  CONCLUSIONS
  Based on the results shown here, we propose a model for the assembly of box C/D snoRNP, according to which R(2)TP complex is important for reducing the affinity of Nop58 for snoRNA, and for the binding of the other snoRNP subunits.
authors.affiliation: Department of Biochemistry, Institute of Chemistry, University of São Paulo, Av. Prof. Lineu Prestes 748, 05508-000, São Paulo, SP, Brazil. marcelinha.bp@gmail.com.
authors.forename: Marcela B
authors.initials: MB
authors.lastname: Prieto
authors.affiliation: Department of Biochemistry, Institute of Chemistry, University of São Paulo, Av. Prof. Lineu Prestes 748, 05508-000, São Paulo, SP, Brazil. rcgeorg@gmail.com.|Present address: Department of Biochemistry and Molecular Biology, Institute of Biological Sciences, Federal University of Goiás, Goiânia, Brazil. rcgeorg@gmail.com.
authors.forename: Raphaela C
authors.initials: RC
authors.lastname: Georg
authors.affiliation: Department of Biochemistry, Institute of Chemistry, University of São Paulo, Av. Prof. Lineu Prestes 748, 05508-000, São Paulo, SP, Brazil. fagonzales@yahoo.com.
authors.forename: Fernando A
authors.initials: FA
authors.lastname: Gonzales-Zubiate
authors.affiliation: Department of Biochemistry, Institute of Chemistry, University of São Paulo, Av. Prof. Lineu Prestes 748, 05508-000, São Paulo, SP, Brazil. jsluz.sp@gmail.com.|Present address: Department of Biological Sciences, School of Pharmacy, São Paulo State University, Araraquara, Brazil. jsluz.sp@gmail.com.
authors.forename: Juliana S
authors.initials: JS
authors.lastname: Luz
authors.affiliation: Department of Biochemistry, Institute of Chemistry, University of São Paulo, Av. Prof. Lineu Prestes 748, 05508-000, São Paulo, SP, Brazil. ccoliv@iq.usp.br.
authors.forename: Carla C
authors.initials: CC
authors.lastname: Oliveira
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D004265:DNA Helicases / Q000378:metabolism; D008958:Models, Molecular; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D055550:Protein Stability; D020718:Ribonucleoproteins, Small Nucleolar / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism
chemical_list: C438199:NOP58 protein, S cerevisiae; D009687:Nuclear Proteins; C497702:PIH1 protein, S cerevisiae; D020718:Ribonucleoproteins, Small Nucleolar; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D000251:Adenosine Triphosphatases; C428198:RVB1 protein, S cerevisiae; C428199:RVB2 protein, S cerevisiae; D004265:DNA Helicases