title: PAT1 inversely regulates the surface Amyloid Precursor Protein level in mouse primary neurons.
abstract: BACKGROUND
  The amyloid precursor protein (APP) is a key molecule in Alzheimer disease. Its localization at the cell surface can trigger downstream signaling and APP cleavages. APP trafficking to the cell surface in neurons is not clearly understood and may be related to the interactions with its partners. In this respect, by having homologies with kinesin light chain domains and because of its capacity to bind APP, PAT1 represents a good candidate.
  
  
  RESULTS
  We observed that PAT1 binds poorly APP at the cell surface of primary cortical neurons contrary to cytoplasmic APP. Using down and up-regulation of PAT1, we observed respectively an increase and decrease of APP at the cell surface. The increase of APP at the cell surface induced by low levels of PAT1 did not trigger cell death signaling.
  
  
  CONCLUSIONS
  These data suggest that PAT1 slows down APP trafficking to the cell surface in primary cortical neurons. Our results contribute to the elucidation of mechanisms involved in APP trafficking in Alzheimer disease.
authors.affiliation: INSERM UMR 894, Université Paris Descartes, Sorbonne Paris Cité, Faculté de Médecine, Paris, France. aysdilsizoglu@yahoo.com.
authors.forename: Aysegul
authors.initials: A
authors.lastname: Dilsizoglu Senol
authors.affiliation: Department of Biochemistry, Biophysics and General Pathology, School of Medicine, Second University of Naples, Naples, Italy. lidiatag@hotmail.it.
authors.forename: Lidia
authors.initials: L
authors.lastname: Tagliafierro
authors.affiliation: INSERM UMR 894, Université Paris Descartes, Sorbonne Paris Cité, Faculté de Médecine, Paris, France. lea.huguet@inserm.fr.
authors.forename: Léa
authors.initials: L
authors.lastname: Huguet
authors.affiliation: INSERM UMR 894, Université Paris Descartes, Sorbonne Paris Cité, Faculté de Médecine, Paris, France. lucie.gorisse@inserm.fr.
authors.forename: Lucie
authors.initials: L
authors.lastname: Gorisse-Hussonnois
authors.affiliation: INSERM UMR 894, Université Paris Descartes, Sorbonne Paris Cité, Faculté de Médecine, Paris, France. stephanie.chasseigneaux@parisdescartes.fr.|Inserm, U1144, Paris, F-75006, France. stephanie.chasseigneaux@parisdescartes.fr.|Université Paris Descartes, UMR-S 1144, Paris, F-75006, France. stephanie.chasseigneaux@parisdescartes.fr.|Université Paris Diderot, UMR-S 1144, Paris, F-75013, France. stephanie.chasseigneaux@parisdescartes.fr.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Chasseigneaux
authors.affiliation: INSERM UMR 894, Université Paris Descartes, Sorbonne Paris Cité, Faculté de Médecine, Paris, France. bernadette.allinquant@inserm.fr.
authors.forename: Bernadette
authors.initials: B
authors.lastname: Allinquant
mesh_terms: D026905:Amino Acid Transport Systems / Q000235:genetics / Q000378:metabolism*; D016564:Amyloid beta-Protein Precursor / Q000378:metabolism*; D000818:Animals; D019921:Biotinylation; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D015536:Down-Regulation; D004926:Escherichia coli; D006801:Humans; D051379:Mice; D009474:Neurons / Q000378:metabolism*; D034741:RNA, Small Interfering; D027981:Symporters / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D026905:Amino Acid Transport Systems; D016564:Amyloid beta-Protein Precursor; D034741:RNA, Small Interfering; C475905:SLC36A1 protein, human; C483904:Slc36a1 protein, mouse; D027981:Symporters