title: Extraction of polyhydroxyalkanoates from mixed microbial cultures: Impact on polymer quality and recovery.
abstract: Polyhydroxyalkanoates (PHAs) can be extracted from mixed microbial cultures (MMCs) by means of dimethyl carbonate (DMC) or combination of DMC and sodium hypochlorite (NaClO). The protocol based on DMC, a green solvent never used before for the extraction of PHAs from MMC, allows an overall polymer recovery of 63%; also the purity and the molecular weight of the recovered polymers are good (98% and 1.2 MDa, respectively). The use of NaClO pretreatment before DMC extraction increases the overall PHA recovery (82%) but lowers the mean molecular weight to 0.6-0.2 MDa. A double extraction with DMC results to be the method of choice for the recovery of high quality PHAs from attractive but challenging MMCs.
authors.affiliation: Centro Interdipartimentale di Ricerca Industriale Energia Ambiente (CIRI EA), University of Bologna, via S. Alberto 163, 48123 Ravenna, Italy. Electronic address: chiara.samori3@unibo.it.
authors.forename: Chiara
authors.initials: C
authors.lastname: Samorì
authors.affiliation: Centro Interdipartimentale di Ricerca Industriale Energia Ambiente (CIRI EA), University of Bologna, via S. Alberto 163, 48123 Ravenna, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Abbondanzi
authors.affiliation: Centro Interdipartimentale di Ricerca Industriale Energia Ambiente (CIRI EA), University of Bologna, via S. Alberto 163, 48123 Ravenna, Italy; Dipartimento di Chimica "Giacomo Ciamician", University of Bologna, via Selmi 2, Bologna, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Galletti
authors.affiliation: Dipartimento di Chimica Industriale "Toso Montanari", University of Bologna, viale del Risorgimento 4, Bologna, Italy; Centro Interdipartimentale di Ricerca Industriale per la Meccanica Avanzata e i Materiali (CIRI MAM), University of Bologna, Viale Risorgimento 2, Bologna, Italy.
authors.forename: Loris
authors.initials: L
authors.lastname: Giorgini
authors.affiliation: Centro Interdipartimentale di Ricerca Industriale per la Meccanica Avanzata e i Materiali (CIRI MAM), University of Bologna, Viale Risorgimento 2, Bologna, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Mazzocchetti
authors.affiliation: Centro Interdipartimentale di Ricerca Industriale Energia Ambiente (CIRI EA), University of Bologna, via S. Alberto 163, 48123 Ravenna, Italy; Dipartimento di Chimica "Giacomo Ciamician", University of Bologna, via Selmi 2, Bologna, Italy.
authors.forename: Cristian
authors.initials: C
authors.lastname: Torri
authors.affiliation: Centro Interdipartimentale di Ricerca Industriale Energia Ambiente (CIRI EA), University of Bologna, via S. Alberto 163, 48123 Ravenna, Italy; Dipartimento di Chimica "Giacomo Ciamician", University of Bologna, via Selmi 2, Bologna, Italy.
authors.forename: Emilio
authors.initials: E
authors.lastname: Tagliavini
mesh_terms: D001419:Bacteria / Q000254:growth & development / Q000378:metabolism*; D061249:Batch Cell Culture Techniques; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D054813:Polyhydroxyalkanoates / Q000302:isolation & purification*; D012997:Solvents
chemical_list: D054813:Polyhydroxyalkanoates; D012997:Solvents
keywords: Dimethyl carbonate; Mixed microbial cultures; Polyhydroxyalkanoates; Polymer extraction