title: Peripheral vagus nerve stimulation significantly affects lipid composition and protein secondary structure within dopamine-related brain regions in rats.
abstract: Recent immunohistochemical studies point to the dorsal motor nucleus of the vagus nerve as the point of departure of initial changes which are related to the gradual pathological developments in the dopaminergic system. In the light of current investigations, it is likely that biochemical changes within the peripheral nervous system may influence the physiology of the dopaminergic system, suggesting a putative role for it in the development of neurodegenerative disorders. By using Fourier transform infrared microspectroscopy, coupled with statistical analysis, we examined the effect of chronic, unilateral electrical vagus nerve stimulation on changes in lipid composition and in protein secondary structure within dopamine-related brain structures in rats. It was found that the chronic vagal nerve stimulation strongly affects the chain length of fatty acids within the ventral tegmental area, nucleus accumbens, substantia nigra, striatum, dorsal motor nucleus of vagus and the motor cortex. In particular, the level of lipid unsaturation was found significantly increasing in the ventral tegmental area, substantia nigra and motor cortex as a result of vagal nerve stimulation. When it comes to changes in protein secondary structure, we could see that the mesolimbic, mesocortical and nigrostriatal dopaminergic pathways are particularly affected by vagus nerve stimulation. This is due to the co-occurrence of statistically significant changes in the content of non-ordered structure components, alpha helices, beta sheets, and the total area of Amide I. Macromolecular changes caused by peripheral vagus nerve stimulation may highlight a potential connection between the gastrointestinal system and the central nervous system in rat during the development of neurodegenerative disorders.
authors.affiliation: AGH University of Science and Technology, Faculty of Physics and Applied Computer Science, Al. Mickiewicza 30, 30-059, Kraków, Poland, asurowka@agh.edu.pl.
authors.forename: Artur Dawid
authors.initials: AD
authors.lastname: Surowka
authors.forename: Anna
authors.initials: A
authors.lastname: Krygowska-Wajs
authors.forename: Agata
authors.initials: A
authors.lastname: Ziomber
authors.forename: Piotr
authors.initials: P
authors.lastname: Thor
authors.forename: Adrian Andrzej
authors.initials: AA
authors.lastname: Chrobak
authors.forename: Magdalena
authors.initials: M
authors.lastname: Szczerbowska-Boruchowska
mesh_terms: D000818:Animals; D017776:Autonomic Pathways / Q000502:physiology; D001923:Brain Chemistry; D059290:Dopaminergic Neurons / Q000737:chemistry*; D004525:Efferent Pathways / Q000502:physiology; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D005767:Gastrointestinal Diseases / Q000503:physiopathology; D041981:Gastrointestinal Tract / Q000294:innervation; D008055:Lipids / Q000032:analysis*; D008297:Male; D009419:Nerve Tissue Proteins / Q000737:chemistry*; D017433:Protein Structure, Secondary; D051381:Rats; D017208:Rats, Wistar; D012201:Reward; D017550:Spectroscopy, Fourier Transform Infrared; D055536:Vagus Nerve Stimulation; D017557:Ventral Tegmental Area / Q000502:physiology
chemical_list: D005231:Fatty Acids, Unsaturated; D008055:Lipids; D009419:Nerve Tissue Proteins