title: Genetic differentiation in Indian Major Carp, Cirrhinus mrigala (Hamilton, 1822) from Indian Rivers, as revealed by direct sequencing analysis of mitochondrial Cytochrome b region.
abstract: A 307 bp segment of Cytochrome b gene of mtDNA was sequenced and analyzed for 90 individuals of Cirrhinus mrigala collected across the three rivers, namely Ganges, Narmada and Brahmaputra. Analyses revealed the presence of 14 haplotypes with haplotype diversity (h) ranging from 0.304 to 0.692, and nucleotide diversity (π) 0.002-0.043. The majority of variation was found within the population (96.21%), and the FST value (0.035) as well as the value of exact test of population differentiation (0.893) were found to be insignificant (p<0.05). Analysis of molecular variance (AMOVA) also indicated insignificant differentiation among sub-populations. Generally, low genetic differences were observed even though those populations were from different geographic locations. The present study suggests a single panmictic population of C. mrigala across the three rivers of India.
authors.affiliation: Central Inland Fisheries Research Institute, Barrackpore , Kolkata, West Bengal , India .
authors.forename: Bijay Kumar
authors.initials: BK
authors.lastname: Behera
authors.forename: Swaraj Priyaranjan
authors.initials: SP
authors.lastname: Kunal
authors.forename: Prasenjit
authors.initials: P
authors.lastname: Paria
authors.forename: Priyanka
authors.initials: P
authors.lastname: Das
authors.forename: Dharmendra Kumar
authors.initials: DK
authors.lastname: Meena
authors.forename: Sudip
authors.initials: S
authors.lastname: Pakrashi
authors.forename: Amiya Kumar
authors.initials: AK
authors.lastname: Sahoo
authors.forename: Debabrata
authors.initials: D
authors.lastname: Panda
authors.forename: Joykrushna
authors.initials: J
authors.lastname: Jena
authors.forename: Anil Prakash
authors.initials: AP
authors.lastname: Sharma
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001482:Base Composition; D002347:Carps / Q000235:genetics*; D045303:Cytochromes b / Q000737:chemistry / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000032:analysis* / Q000235:genetics; D040961:Genetic Drift; D014644:Genetic Variation; D006239:Haplotypes; D007194:India; D011110:Polymorphism, Genetic; D045483:Rivers; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial; D045303:Cytochromes b
keywords: Cirrhinus mrigala; Cytochrome b; Indian rivers; genetic differentiation