title: Raman microspectroscopy of nanodiamond-induced structural changes in albumin.
abstract: Nanodiamonds (NDs) are promising agents for theranostic applications due to reported low toxicity and high biocompatibility, which is still being extensively tested on cellular, tissue, and organism levels. It is presumed that for experimental and future clinical applications, NDs will be administered into the organism via the blood circulation system. In this regard, the interaction of NDs with blood components needs to be thoroughly studied. We studied the interaction of carboxylated NDs (cNDs) with albumin, one of the major proteins of blood plasma. After 2-h long in vitro incubation in an aqueous solution of the protein, 100-nm cNDs were dried and the dry samples were studied with the aid of Raman microspectroscopy. The spectroscopic data indicate significant conformational changes that can be due to cND–protein interaction. A possible decrease in the functional activity of albumin related to the conformational changes must be taken into account in the in vivo applications.
authors.affiliation: Lomonosov Moscow State University, Physics Department, Leninskie Gory 1/62, Moscow 119991, Russia.
authors.forename: Anastasiya S
authors.initials: AS
authors.lastname: Svetlakova
authors.affiliation: Lomonosov Moscow State University, Physics Department, Leninskie Gory 1/62, Moscow 119991, Russia.
authors.forename: Nikolay N
authors.initials: NN
authors.lastname: Brandt
authors.affiliation: Lomonosov Moscow State University, Physics Department, Leninskie Gory 1/62, Moscow 119991, RussiabLomonosov Moscow State University, International Laser Center, Leninskie Gory 1/62, Moscow 119992, Russia.
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Priezzhev
authors.affiliation: Lomonosov Moscow State University, International Laser Center, Leninskie Gory 1/62, Moscow 119992, Russia.
authors.forename: Andrey Yu
authors.initials: AY
authors.lastname: Chikishev
mesh_terms: D006801:Humans; D008422:Materials Testing; D058612:Nanodiamonds / Q000737:chemistry* / Q000648:ultrastructure*; D011487:Protein Conformation; D012709:Serum Albumin / Q000737:chemistry* / Q000648:ultrastructure*; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D058612:Nanodiamonds; D012709:Serum Albumin