title: Early effects comparison of X-rays delivered at high-dose-rate pulses by a plasma focus device and at low dose rate on human tumour cells.
abstract: A comparative study has been performed on the effects of high-dose-rate (DR) X-ray beams produced by a plasma focus device (PFMA-3), to exploit its potential medical applications (e.g. radiotherapy), and low-DR X-ray beams produced by a conventional source (XRT). Experiments have been performed at 0.5 and 2 Gy doses on a human glioblastoma cell line (T98G). Cell proliferation rate and potassium outward currents (IK) have been investigated by time lapse imaging and patch clamp recordings. The results showed that PFMA-3 irradiation has a greater capability to reduce the proliferation rate activity with respect to XRT, while it does not affect IK of T98G cells at any of the dose levels tested. XRT irradiation significantly reduces the mean IK amplitude of T98G cells only at 0.5 Gy. This work confirms that the DR, and therefore the source of radiation, is crucial for the planning and optimisation of radiotherapy applications.
authors.affiliation: Department of Physics and Astronomy, University of Bologna, viale C. Berti Pichat 6/2, Bologna 40127, Italy IEO, Via G. Ripamonti 435, Milano 20141, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Virelli
authors.affiliation: Department of Physics and Astronomy, University of Bologna, viale C. Berti Pichat 6/2, Bologna 40127, Italy INFN, Viale C. Berti Pichat 6/2, Bologna 40127, Italy.
authors.forename: I
authors.initials: I
authors.lastname: Zironi
authors.affiliation: Department of Biology and Biotechnology 'L. Spallanzani', University of Pavia, Via Ferrata 9, Pavia 27100, Italy IEO, Via G. Ripamonti 435, Milano 20141, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Pasi
authors.affiliation: Department of Industrial Engineering, University of Bologna, Via dei colli 16, Bologna 40136, Italy LAINSA Italia s.r.l, Via Carlo Porta, 3, Gallarate, VA 21013, USA.
authors.forename: E
authors.initials: E
authors.lastname: Ceccolini
authors.affiliation: Department of Biology and Biotechnology 'L. Spallanzani', University of Pavia, Via Ferrata 9, Pavia 27100, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Nano
authors.affiliation: CNAO Foundation, Strada Campeggi, 53, Pavia 27100, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Facoetti
authors.affiliation: INFN, Viale C. Berti Pichat 6/2, Bologna 40127, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Gavoçi
authors.affiliation: CNAO Foundation, Strada Campeggi, 53, Pavia 27100, Italy.
authors.forename: M R
authors.initials: MR
authors.lastname: Fiore
authors.affiliation: ENEA, Via Martiri di Monte Sole, 4, Bologna 40129, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Rocchi
authors.affiliation: Department of Industrial Engineering, University of Bologna, Via dei colli 16, Bologna 40136, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Mostacci
authors.affiliation: Department of Industrial Engineering, University of Bologna, Via dei colli 16, Bologna 40136, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Cucchi
authors.affiliation: Department of Physics and Astronomy, University of Bologna, viale C. Berti Pichat 6/2, Bologna 40127, Italy INFN, Viale C. Berti Pichat 6/2, Bologna 40127, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Castellani
authors.affiliation: INFN, Viale C. Berti Pichat 6/2, Bologna 40127, Italy Department of Industrial Engineering, University of Bologna, Via dei colli 16, Bologna 40136, Italy marco.sumini@unibo.it.
authors.forename: M
authors.initials: M
authors.lastname: Sumini
authors.affiliation: IEO, Via G. Ripamonti 435, Milano 20141, Italy CNAO Foundation, Strada Campeggi, 53, Pavia 27100, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Orecchia
mesh_terms: D049109:Cell Proliferation / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005909:Glioblastoma / Q000378:metabolism / Q000473:pathology / Q000532:radiotherapy*; D006801:Humans; D018408:Patch-Clamp Techniques; D058626:Plasma Gases / Q000737:chemistry*; D011188:Potassium / Q000378:metabolism*; D011879:Radiotherapy Dosage; D014964:X-Ray Therapy / Q000295:instrumentation* / Q000379:methods*
chemical_list: D058626:Plasma Gases; D011188:Potassium