title: Vanadate inhibits dexamethasone-induced apoptosis of rat bone marrow-derived mesenchymal stem cells.
abstract: Apoptosis of bone marrow-derived mesenchymal stem cells (BM-MSCs) has been shown to contribute to the development of osteoporosis, which is often the result of long-term use of glucocorticoid drugs such as dexamethasone (Dex). However, it remains unknown whether Dex induces apoptosis of BM-MSCs, and whether a chemical agent like vanadate can block such effects. To investigate these two issues, we isolated BM-MSCs from SD rats and treated the cells with different doses of Dex. We found that Dex induced apoptosis in dose- and time-dependent manners. Pretreating BM-MSCs with vanadate prevented Dex-induced apoptosis. Furthermore, we found that expression of caspases (3, 8, and 9) increased in Dex-treated BM-MSC and was attenuated by vanadate pretreatment. These results not only demonstrate the role of vanadate in the inhibition of Dex-induced apoptosis of BM-MSCs, but also reveal the therapeutic potential of vanadate in glucocorticoid-mediated osteoporosis.
authors.affiliation: Department of Spinal Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi.
authors.forename: Qie
authors.initials: Q
authors.lastname: Fan
authors.affiliation: Department of Spinal Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi.
authors.forename: Xinli
authors.initials: X
authors.lastname: Zhan
authors.affiliation: Department of Traumatic Orthopedics and Hand Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Traumatic Orthopedics and Hand Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi ZhaoJinmin2013@163.com.
authors.forename: Jinmin
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Orthopedics, Affiliated Rui Kang Hospital of Guangxi Traditional Chinese Medical School, Nanning Guangxi, China.
authors.forename: Yueping
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001854:Bone Marrow Cells / Q000166:cytology*; D020169:Caspases / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D003907:Dexamethasone / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D051381:Rats; D014638:Vanadates / Q000494:pharmacology*
chemical_list: D014638:Vanadates; D003907:Dexamethasone; D020169:Caspases
keywords: BM-MSCs; apoptosis; caspase; glucocorticoid; vanadate