title: Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next-generation sequencing and cross-amplification from related species.
abstract: OBJECTIVE
  We present a set of 23 polymorphic nuclear microsatellite loci, 18 of which are identified for the first time within the riparian species Salix humboldtiana (Salicaceae) using next-generation sequencing.
  
  
  RESULTS
  To characterize the 23 loci, up to 60 individuals were sampled and genotyped at each locus. The number of alleles ranged from two to eight, with an average of 4.43 alleles per locus. The effective number of alleles ranged from 1.15 to 3.09 per locus, and allelic richness ranged from 2.00 to 7.73 alleles per locus.
  
  
  CONCLUSIONS
  The new marker set will be used for future studies of genetic diversity and differentiation as well as for unraveling spatial genetic structures in S. humboldtiana populations in northern Patagonia, Argentina.
authors.affiliation: Unidad de Genética Ecológica y Mejoramiento Forestal, Instituto Nacional de Tecnología Agropecuaria (INTA), E.E.A. Bariloche, Modesta Victoria 4450, Bariloche 8400, Argentina.
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Bozzi
authors.affiliation: Faculty of Biology, Conservation Biology Group, University of Marburg, Karl-von-Frisch-Str. 8, 35043 Marburg, Germany.
authors.forename: Sascha
authors.initials: S
authors.lastname: Liepelt
authors.affiliation: Faculty of Biology, Conservation Biology Group, University of Marburg, Karl-von-Frisch-Str. 8, 35043 Marburg, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Ohneiser
authors.affiliation: Unidad de Genética Ecológica y Mejoramiento Forestal, Instituto Nacional de Tecnología Agropecuaria (INTA), E.E.A. Bariloche, Modesta Victoria 4450, Bariloche 8400, Argentina.
authors.forename: Leonardo A
authors.initials: LA
authors.lastname: Gallo
authors.affiliation: Unidad de Genética Ecológica y Mejoramiento Forestal, Instituto Nacional de Tecnología Agropecuaria (INTA), E.E.A. Bariloche, Modesta Victoria 4450, Bariloche 8400, Argentina ; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Rivadavia 1917, Ciudad Autónoma de Buenos Aires C1092AAR, Argentina.
authors.forename: Paula
authors.initials: P
authors.lastname: Marchelli
authors.affiliation: Faculty of Biology, Conservation Biology Group, University of Marburg, Karl-von-Frisch-Str. 8, 35043 Marburg, Germany ; Institute of Botany, Plant Ecology and Nature Conservation, University of Geisenheim, Von-Lade-Str. 1, 65366 Geisenheim, Germany.
authors.forename: Ilona
authors.initials: I
authors.lastname: Leyer
authors.affiliation: Faculty of Biology, Conservation Biology Group, University of Marburg, Karl-von-Frisch-Str. 8, 35043 Marburg, Germany.
authors.forename: Birgit
authors.initials: B
authors.lastname: Ziegenhagen
authors.affiliation: Faculty of Biology, Conservation Biology Group, University of Marburg, Karl-von-Frisch-Str. 8, 35043 Marburg, Germany.
authors.forename: Christina
authors.initials: C
authors.lastname: Mengel
keywords: Salicaceae; Salix humboldtiana; next-generation sequencing; nuclear microsatellite marker; river margins