title: A facile ultrasonication assisted method for Fe3O4@SiO2-Ag nanospheres with excellent antibacterial activity.
abstract: To increase the monodispersity of magnetic hybrid nanocomposites, a novel ultrasonic method was introduced to synthesize uniform Fe3O4@SiO2-Ag nanospheres. The immobilized Ag nanocrystals were tunable by varying the experimental conditions. An antibacterial assay indicated that the Fe3O4@SiO2-Ag nanospheres exhibited excellent antibacterial activities against Staphylococcus aureus and Escherichia coli, in which the minimum inhibition concentrations (MIC) were 40 μg mL(-1) and 20 μg mL(-1), respectively. The live/dead bacterial cell fluorescence stain assay agreed well with the antibacterial assay. The CCK-8 results indicated these nanospheres were bio-compatible for human normal cells and presented relative cytotoxicity against HepG2 tumor cells. These nanospheres could be easily uptaken by the cells and they could affect bacterial cells both inside and outside the cell membrane, which enable them to be promisingly applied in future biomedical areas.
authors.affiliation: Department of Chirurgery, Affiliated Hospital 1, Anhui Medical University, Hefei 230032, P.R. China. yugj7806290@sohu.com xiongqiru2012@126.com.
authors.forename: Shuang-Sheng
authors.initials: SS
authors.lastname: Chen
authors.forename: Hui
authors.initials: H
authors.lastname: Xu
authors.forename: Hua-Jian
authors.initials: HJ
authors.lastname: Xu
authors.forename: Guang-Jin
authors.initials: GJ
authors.lastname: Yu
authors.forename: Xing-Long
authors.initials: XL
authors.lastname: Gong
authors.forename: Qun-Ling
authors.initials: QL
authors.lastname: Fang
authors.forename: Ken Cham-Fai
authors.initials: KC
authors.lastname: Leung
authors.forename: Shou-Hu
authors.initials: SH
authors.lastname: Xuan
authors.forename: Qi-Ru
authors.initials: QR
authors.lastname: Xiong
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D052203:Ferrosoferric Oxide / Q000737:chemistry / Q000494:pharmacology*; D056945:Hep G2 Cells; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects; D006801:Humans; D008826:Microbial Sensitivity Tests; D054118:Nanospheres / Q000737:chemistry*; D010316:Particle Size; D012822:Silicon Dioxide / Q000737:chemistry / Q000494:pharmacology*; D012834:Silver / Q000737:chemistry / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development; D013329:Structure-Activity Relationship; D013499:Surface Properties; D014465:Ultrasonics
chemical_list: D000900:Anti-Bacterial Agents; D012834:Silver; D012822:Silicon Dioxide; D052203:Ferrosoferric Oxide