title: Conformational behavior of polyalanine peptides with and without protecting groups of varying chain lengths: population of PP-II structure!
abstract: Oculopharyngeal muscular dystrophy (OPMD), a polyalanine myopathy, occurs due to expansion of homo-polyalanine stretch in normal polyadenylating binding protein nuclear 1 (PABPN1) protein from Ala10 to Ala11-17. Therefore, the conformational behavior of polyalanine peptides with n = 10-17, with and without terminal protecting groups, have been investigated with different starting geometries in water by molecular dynamics simulation studies. Alanine peptides are shown to give rise to unordered structure irrespective of starting geometry and not more than two residues at a stretch have the same/similar set of φ, ψ values. However, the final structure with terminal protecting groups look like β-strand. Unprotected poly-Ala peptides adopt twisted β-hairpin/multi hairpin like structure with increasing chain length. The number of residues having φ, ψ values in collagen region is found to be less in peptides with unprotected termini as compared to peptides with protected termini of same chain length. The results have been supported by recent synchrotron radiation circular dichroism spectroscopy of polyproline II and unordered secondary structures. Opening of the helical structure in poly-Ala peptides with protecting groups has been shown to take place from C-terminal and in peptides without protecting groups opening of helix starts from both terminals. Further, opening of helix takes more time in poly-Ala peptides without terminal protecting groups. The deviations in amide bond planarity have been discussed and compared with available experimental and computational results.
authors.affiliation: Department of Biophysics, Panjab University, Chandigarh, 160014, India, fateh_nandel@yahoo.com.
authors.forename: Fateh S
authors.initials: FS
authors.lastname: Nandel
authors.forename: Mohan L
authors.initials: ML
authors.lastname: Garg
authors.forename: Mohd
authors.initials: M
authors.lastname: Shafique
mesh_terms: D000577:Amides / Q000737:chemistry; D006801:Humans; D056004:Molecular Dynamics Simulation; D039141:Muscular Dystrophy, Oculopharyngeal / Q000235:genetics* / Q000473:pathology; D010455:Peptides / Q000737:chemistry* / Q000235:genetics; D039122:Poly(A)-Binding Protein I / Q000737:chemistry* / Q000235:genetics; D011487:Protein Conformation; D017433:Protein Structure, Secondary
chemical_list: D000577:Amides; C576588:PABPN1 protein, human; D010455:Peptides; D039122:Poly(A)-Binding Protein I; C011083:polyproline; C019529:polyalanine