title: Immunoproteomic identification of immunodominant antigens independent of the time of infection in Brucella abortus 2308-challenged cattle.
abstract: Brucellosis is a vital zoonotic disease caused by Brucella, which infects a wide range of animals and humans. Accurate diagnosis and reliable vaccination can control brucellosis in domestic animals. This study examined novel immunogenic proteins that can be used to detect Brucella abortus infection or as an effective subcellular vaccine. In an immunoproteomic assay, 55 immunodominant proteins from B. abortus 544 were observed using two dimensional electrophoresis (2DE) and immunoblot profiles with antisera from B. abortus-infected cattle at the early (week 3), middle (week 7), and late (week 10) periods, after excluding protein spots reacting with antisera from Yersinia enterocolitica O:9-infected and non-infected cattle. Twenty-three selected immunodominant proteins whose spots were observed at all three infection periods were identified using MALDI-MS/MS. Most of these proteins identified by immunoblot and mass spectrometry were determined by their subcellular localization and predicted function. We suggest that the detection of prominent immunogenic proteins during the infection period can support the development of advanced diagnostic methods with high specificity and accuracy; subsidiarily, these proteins can provide supporting data to aid in developing novel vaccine candidates.
authors.affiliation: Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do, 430-757, Republic of Korea. lejjj84@korea.kr.
authors.forename: Jin Ju
authors.initials: JJ
authors.lastname: Lee
authors.affiliation: Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, 660-701, Republic of Korea. hlsimborio@yahoo.com.
authors.forename: Hannah Leah
authors.initials: HL
authors.lastname: Simborio
authors.affiliation: Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, 660-701, Republic of Korea. awbreyes@gmail.com.
authors.forename: Alisha Wehdnesday Bernardo
authors.initials: AW
authors.lastname: Reyes
authors.affiliation: Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, 660-701, Republic of Korea. romanticvet@nate.com.
authors.forename: Dae Geun
authors.initials: DG
authors.lastname: Kim
authors.affiliation: Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, 660-701, Republic of Korea. hoparti@gmail.com.
authors.forename: Huynh Tan
authors.initials: HT
authors.lastname: Hop
authors.affiliation: Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, 660-701, Republic of Korea. wongimin@gnu.ac.kr.
authors.forename: Wongi
authors.initials: W
authors.lastname: Min
authors.affiliation: Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do, 430-757, Republic of Korea. herm@korea.kr.
authors.forename: Moon
authors.initials: M
authors.lastname: Her
authors.affiliation: Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do, 430-757, Republic of Korea. brujung@korea.kr.
authors.forename: Suk Chan
authors.initials: SC
authors.lastname: Jung
authors.affiliation: Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul, 151-742, Republic of Korea. yoohs@snu.ac.kr.
authors.forename: Han Sang
authors.initials: HS
authors.lastname: Yoo
authors.affiliation: Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, 660-701, Republic of Korea. kimsuk@gnu.ac.kr.|Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 660-701, Republic of Korea. kimsuk@gnu.ac.kr.
authors.forename: Suk
authors.initials: S
authors.lastname: Kim
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D002003:Brucella abortus / Q000276:immunology*; D002007:Brucellosis, Bovine / Q000276:immunology*; D002417:Cattle; D005260:Female; D007106:Immune Sera / Q000276:immunology*; D015151:Immunoblotting / Q000662:veterinary; D016056:Immunodominant Epitopes / Q000276:immunology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000662:veterinary; D053719:Tandem Mass Spectrometry / Q000662:veterinary
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007106:Immune Sera; D016056:Immunodominant Epitopes