title: Structural and phase characterization of bioceramics prepared from tetracalcium phosphate-monetite cement and in vitro osteoblast response.
abstract: Biphasic porous calcium phosphate ceramics was prepared by sintering of transformed tetracalcium phosphate-monetite cement. After annealing hydroxyapatite, β- or α-TCP were found as main phases in ceramic substrates and a highly microporous microstructure of cement ceramics was created without an addition of porosifier. The origin microstructure features characteristic by the presence of hollow particle agglomerates in cement were preserved in microstructure of cement ceramics after annealing but the hydroxyapatite particles rose in size up to 2 µm and obtained a more regular shape. A small decrease in compressive strength was demonstrated in ceramics sintered up to 1150 °C and enhanced osteoblast proliferation was revealed on cement ceramic substrates in comparison with cement sample and conventional ceramics. The ALP activity of osteoblasts decreased with rise in sintering temperature. The prepared cement microporous ceramics could be utilized as carrier for antibiotics, drugs, growth factors, enzymes or other substances stimulating healing process.
authors.affiliation: Institute of Materials Research of SAS, Watsonova 47, 04001, Kosice, Slovakia.
authors.forename: Radoslava
authors.initials: R
authors.lastname: Stulajterova
authors.forename: Lubomir
authors.initials: L
authors.lastname: Medvecky
authors.forename: Maria
authors.initials: M
authors.lastname: Giretova
authors.forename: Tibor
authors.initials: T
authors.lastname: Sopcak
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001843:Bone Cements / Q000737:chemistry*; D002130:Calcium Phosphates / Q000737:chemistry*; D049109:Cell Proliferation / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002516:Ceramics / Q000737:chemistry*; D019245:Compressive Strength; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006244:Hardness; D006361:Heating; D008422:Materials Testing; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects / Q000502:physiology*; D016062:Porosity; D054457:Tissue Scaffolds
chemical_list: D001843:Bone Cements; D002130:Calcium Phosphates; C485830:tetracalcium phosphate; C485829:calcium phosphate, dibasic, anhydrous