title: Expression of important pathway genes involved in withanolides biosynthesis in hairy root culture of Withania somnifera upon treatment with Gracilaria edulis and Sargassum wightii.
abstract: The investigation of seaweeds, Gracilaria edulis and Sargassum wightii extracts was carried out for the estimation of growth characteristics and major withanolides production in hairy root culture of Withania somnifera. The extract of G. edulis (50%) in MS liquid basal medium enabled maximum production of dry biomass (5.46 g DW) and withanolides contents (withanolide A 5.23 mg/g DW; withaferin A 2.24 mg/g DW and withanone 4.83 mg/g DW) in hairy roots after 40 days of culture with 48 h contact time. The obtained withanolides contents were significantly higher (2.32-fold-2.66-fold) in hairy root culture when compared to the control. RT PCR analysis of important pathway genes such as SE, SS, HMGR and FPPS exhibited substantial higher expression upon the seaweed extracts treatment in hairy root culture. This experiment would paw a platform for withanolides production in hairy root culture with the influence of sea weed extracts for pharmaceutical companies in the future.
authors.affiliation: Department of Biotechnology and Genetic Engineering, School of Biotechnology, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India; Molecular Genetics and Genomics Laboratory, Department of Horticulture, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 305 764, South Korea. Electronic address: gsivabdu@gmail.com.
authors.forename: Ganeshan
authors.initials: G
authors.lastname: Sivanandhan
authors.affiliation: Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
authors.forename: Chinnathambi
authors.initials: C
authors.lastname: Arunachalam
authors.affiliation: Department of Botany, Periyar E.V.R College (Autonomous), Tiruchirappalli 620 023, Tamil Nadu, India.
authors.forename: Natesan
authors.initials: N
authors.lastname: Selvaraj
authors.affiliation: Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
authors.forename: Ali Alharbi
authors.initials: AA
authors.lastname: Sulaiman
authors.affiliation: Molecular Genetics and Genomics Laboratory, Department of Horticulture, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 305 764, South Korea.
authors.forename: Yong Pyo
authors.initials: YP
authors.lastname: Lim
authors.affiliation: Department of Biotechnology and Genetic Engineering, School of Biotechnology, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India. Electronic address: aganapathi2003@rediffmail.com.
authors.forename: Andy
authors.initials: A
authors.lastname: Ganapathi
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000737:chemistry; D018506:Gene Expression Regulation, Plant; D044724:Gracilaria / Q000737:chemistry*; D007194:India; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism*; D044647:Sargassum / Q000737:chemistry*; D012624:Seaweed / Q000737:chemistry; D032341:Withania / Q000235:genetics / Q000378:metabolism*; D054358:Withanolides / Q000737:chemistry
chemical_list: D003470:Culture Media; D010936:Plant Extracts; D054358:Withanolides; C009684:withaferin A
keywords: Gracilaria edulis; Hairy root culture; Sargassum wightii; Withania somnifera; Withanolides