title: Reduced miR-29a-3p expression is linked to the cell proliferation and cell migration in gastric cancer.
abstract: BACKGROUND
  MicroRNAs (miRNAs) play an important role in a tumor-suppressive or oncogenic manner in carcinogenesis. Alteration expression patterns of miRNAs in gastric cancer (GC) are associated with cancer initiation and progression. In the present study, we evaluated miR-29a-3p expression pattern and its function in gastric carcinogenesis.
  
  
  METHODS
  The expression of miR-29a-3p in GC tissue samples and cell lines was detected by quantitative real-time PCR (qRT-PCR). After transfected with miR-29a-3p mimics or inhibitor, the cell proliferation, cell migration, and invasion ability were assessed by CCK-8 assay, wound healing assay, and Trans-well assay, respectively. The level of CDK2, CDK4, CDK6, and CyclinD1 were determined by qRT-PCR and Western blot.
  
  
  RESULTS
  Compared with the corresponding non-tumor tissues, miR-29a-3p showed a significant down-regulated expression in tumor tissues. In vitro functional assays demonstrated that enforced miR-29a-3p expression inhibited cell proliferation by reducing the expression of CDK2, CDK4, and CDK6. Wound healing and Transwell assays revealed that miR-29a-3p suppressed tumor metastasis in GC.
  
  
  CONCLUSIONS
  Our preliminary results suggest that altered expression of miR-29a-3p is involved in gastric cancer process. The present study provides the first insight into the specific role of miR-29a-3p in gastric carcinogenesis.
authors.affiliation: Department of Genetics & Developmental Biology, the Medical School of Southeast University and Key Laboratory of Developmental Genes and Human Diseases, Ministry of Education, Southeast University, 2 Sipailou, Xuanwu, Nanjing, 210009, China. zhujiangzhao@seu.edu.cn.
authors.forename: Zhujiang
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Department of Genetics & Developmental Biology, the Medical School of Southeast University and Key Laboratory of Developmental Genes and Human Diseases, Ministry of Education, Southeast University, 2 Sipailou, Xuanwu, Nanjing, 210009, China. wangling1615@126.com.
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Genetics & Developmental Biology, the Medical School of Southeast University and Key Laboratory of Developmental Genes and Human Diseases, Ministry of Education, Southeast University, 2 Sipailou, Xuanwu, Nanjing, 210009, China. songwei3929@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Song
authors.affiliation: Department of Genetics & Developmental Biology, the Medical School of Southeast University and Key Laboratory of Developmental Genes and Human Diseases, Ministry of Education, Southeast University, 2 Sipailou, Xuanwu, Nanjing, 210009, China. cuiheseu@163.com.
authors.forename: He
authors.initials: H
authors.lastname: Cui
authors.affiliation: Jiangning Hospital, Nanjing Medical University, 140 Hanzhong Road, Nanjing, 210000, China. otga@sina.com.
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.affiliation: Department of Genetics & Developmental Biology, the Medical School of Southeast University and Key Laboratory of Developmental Genes and Human Diseases, Ministry of Education, Southeast University, 2 Sipailou, Xuanwu, Nanjing, 210009, China. fengchang-qiao@163.com.
authors.forename: Fengchang
authors.initials: F
authors.lastname: Qiao
authors.affiliation: Department of Genetics & Developmental Biology, the Medical School of Southeast University and Key Laboratory of Developmental Genes and Human Diseases, Ministry of Education, Southeast University, 2 Sipailou, Xuanwu, Nanjing, 210009, China. joycehu1120@foxmail.com.
authors.forename: Jiaojiao
authors.initials: J
authors.lastname: Hu
authors.affiliation: Jiangning Hospital, Nanjing Medical University, 140 Hanzhong Road, Nanjing, 210000, China. pinger_523@126.com.
authors.forename: Rongping
authors.initials: R
authors.lastname: Zhou
authors.affiliation: Department of Genetics & Developmental Biology, the Medical School of Southeast University and Key Laboratory of Developmental Genes and Human Diseases, Ministry of Education, Southeast University, 2 Sipailou, Xuanwu, Nanjing, 210009, China. fanh@seu.edu.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Fan
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: C532045:MIRN29a microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger