title: Fluorinated carbon tag derivatization combined with fluorous solid-phase extraction: a new method for the highly sensitive and selective mass spectrometric analysis of glycans.
abstract: The sensitive and specific detection of glycans via mass spectrometry (MS) remains a significant challenge due to their low abundance in complex biological mixtures, inherent lack of hydrophobicity, and suppression by other, more abundant biological molecules (proteins/peptides) or contaminants. A new strategy for the sensitive and selective MS analysis of glycans based on fluorous chemistry is reported. Glycan reducing ends were derivatized with a hydrophobic fluorinated carbon tag, increasing glycan ionization efficiency during MS by more than an order of magnitude. More importantly, the fluorinated carbon tag enabled efficient fluorous solid-phase extraction (FSPE) to specifically enrich the glycans from contaminated solutions and protein mixtures. Finally, we successfully analyzed the N-glycome in human serum using this new method.
authors.affiliation: †Shanghai Cancer Center and Institutes of Biomedical Sciences, and ‡Department of Chemistry and Key Laboratory of Glycoconjugates Research Ministry of Public Health, Fudan University, Shanghai 200032, China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Li
authors.affiliation: †Shanghai Cancer Center and Institutes of Biomedical Sciences, and ‡Department of Chemistry and Key Laboratory of Glycoconjugates Research Ministry of Public Health, Fudan University, Shanghai 200032, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Jiao
authors.affiliation: †Shanghai Cancer Center and Institutes of Biomedical Sciences, and ‡Department of Chemistry and Key Laboratory of Glycoconjugates Research Ministry of Public Health, Fudan University, Shanghai 200032, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Cai
authors.affiliation: †Shanghai Cancer Center and Institutes of Biomedical Sciences, and ‡Department of Chemistry and Key Laboratory of Glycoconjugates Research Ministry of Public Health, Fudan University, Shanghai 200032, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: †Shanghai Cancer Center and Institutes of Biomedical Sciences, and ‡Department of Chemistry and Key Laboratory of Glycoconjugates Research Ministry of Public Health, Fudan University, Shanghai 200032, China.
authors.forename: Haojie
authors.initials: H
authors.lastname: Lu
mesh_terms: D053000:Analytic Sample Preparation Methods; D002236:Carbohydrate Conformation; D002244:Carbon / Q000737:chemistry*; D054879:Halogenation; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008958:Models, Molecular; D011134:Polysaccharides / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D011134:Polysaccharides; D002244:Carbon