title: Comparing Short-Duration Electro-Oculograms with and without Mydriasis in Healthy Subjects.
abstract: BACKGROUND
  In order to obtain artifact-free electro-oculogram recordings the subject's cooperation is necessary. The aim of our study is to evaluate the recording characteristics of short-duration EOG and to compare the effect of mydriasis on electro-oculogram recordings in a cohort of controls.
  
  
  METHODS
  Electro-oculogram recordings were performed on a light-emitting diode stimulus screen using a RETI-port gamma plus2 system (RETIscan™, Roland Consult). Fast oscillations were set at 1.5 sec (6 cycles; total duration 75 sec).The dark phase included: pre-adaptation (6 min), alternate fixation (4 min), fixation-rest (20 sec), 100 sweeps. The light phase included: light adaptation (4 min), alternate fixation (10 min), fixation-rest (20 sec), 250 sweeps. The amplifier band pass was filtered at 0.1÷50 Hz. The background illumination in mydriasis was 100 cd/m2 and in miosis--450 cd/m2.
  
  
  RESULTS
  A total of 55 controls participated and were divided into three age groups [number; mean (years, y); ±SD]: group 1: 18-20 years (19; 19.49 years; ±0.89); group 2: 20-40 years (18; 27.91 years; ±5.39) and group 3: 40-60 years (18; 48.66 years; ±4.00). The Arden ratio, dark-trough and light-peak did not differ between recordings with or without mydriasis (p=0.914; p=0.880; p=0.680, linear mixed-effects model). The age did not influence the Arden ratio, dark-trough, light-peak (p=0.206; p=0.112; p=0.155). Arden ratio, dark-trough, light-peak were comparable between tested eyes (p=0.934; p=0.193; p=0.270).
  
  
  CONCLUSIONS
  Short-duration electro-oculograms allow successful recording, furthermore, the application of mydriasis does not influence the quality of the recording.
authors.affiliation: Department of Ophthalmology, University Hospital Basel, Switzerland (Chairman a. i.: Dr. sc. nat. Norbert Spirig).
authors.forename: C
authors.initials: C
authors.lastname: Türksever
authors.affiliation: Department of Ophthalmology, University Hospital Basel, Switzerland (Chairman a. i.: Dr. sc. nat. Norbert Spirig).
authors.forename: S
authors.initials: S
authors.lastname: Orgül
authors.affiliation: Department of Ophthalmology, University Hospital Basel, Switzerland (Chairman a. i.: Dr. sc. nat. Norbert Spirig).
authors.forename: M G
authors.initials: MG
authors.lastname: Todorova
mesh_terms: D000293:Adolescent; D000328:Adult; D004585:Electrooculography / Q000187:drug effects* / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009184:Mydriatics / Q000008:administration & dosage*; D012016:Reference Values; D015203:Reproducibility of Results; D012160:Retina / Q000187:drug effects* / Q000502:physiology*; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D009184:Mydriatics