title: Synthesis of a hybrid polymer-inorganic biomimetic support incorporating in situ pectinase from Aspergillus niger ATCC 9642.
abstract: The hybrid alginate/gelatin/calcium oxalate (AGOCa) support was successfully synthesized through the biomimetic mineralization method for immobilization in situ of a pectinolytic extract from Aspergillus niger ATCC 9642 via entrapment technique. The efficiency of immobilization reached 72.7%. Sodium oxalate buffer (100 mM, pH 5.5) was selected as adjuvant of the immobilization process by allowing the formation of a calcified shell around the calcium alginate capsule, significantly increasing the stability to storage, thermal and recycling of the enzymatic immobilized pectinolytic extract. The pH and temperature for maximum activity were from 5.0 to 6.0 and 60 to 80 °C, respectively. The new hybrid support can be a potential alternative to obtain immobilized pectinases with properties for advantageous industrial applications.
authors.affiliation: Departamento de Ciências Agrárias, Universidade Regional do Alto Uruguai e das Missões, URI-Erechim, Erechim, RS, 99700-000, Brazil.
authors.forename: Cindy Elena
authors.initials: CE
authors.lastname: Bustamante-Vargas
authors.forename: Marcelo Luis
authors.initials: ML
authors.lastname: Mignoni
authors.forename: Débora
authors.initials: D
authors.lastname: de Oliveira
authors.forename: Luciana Dornelles
authors.initials: LD
authors.lastname: Venquiaruto
authors.forename: Eunice
authors.initials: E
authors.lastname: Valduga
authors.forename: Geciane
authors.initials: G
authors.lastname: Toniazzo
authors.forename: Rogério Marcos
authors.initials: RM
authors.lastname: Dallago
mesh_terms: D000464:Alginates / Q000737:chemistry*; D001234:Aspergillus niger / Q000201:enzymology*; D040761:Biomimetic Materials / Q000138:chemical synthesis* / Q000737:chemistry; D002129:Calcium Oxalate / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry; D005656:Fungal Proteins / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D011096:Polygalacturonase / Q000737:chemistry*
chemical_list: D000464:Alginates; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D006603:Hexuronic Acids; D002129:Calcium Oxalate; D020723:Glucuronic Acid; D005780:Gelatin; D011096:Polygalacturonase