title: MicroRNA-9 suppresses the sensitivity of CNE2 cells to ultraviolet radiation.
abstract: MicroRNA (miR)-9 has been demonstrated to regulate the radiosensitivity of tumor cells. In the present study, the mechanism by which miR-9 modulates the sensitivity of nasopharyngeal carcinoma (NPC) cells to ultraviolet (UV) radiation was investigated. The results demonstrated that exposure of NPC cells to UV light resulted in a significant increase in the expression of miR-9, and that CNE2 cells overexpressing miR-9 exhibited reduced levels of DNA damage and increased levels of total glutathione upon UV exposure. Accordingly, the inhibition of the expression of miR-9 promoted UV-induced DNA damage and apoptosis. Although miR-9 inhibited the expression of E-cadherin in the CNE2 cells and increased their resistance to UV radiation, the use of small interfering RNA to inhibit the expression of E-cadherin was not sufficient to decrease the radiosensitivity of the NPC cells. These data demonstrated that miR-9 did not modulate the sensitivity of the CNE2 cells to UV radiation through E-cadherin, but suggested that miR-9 regulated radiosensitivity through its effects on glutathione. These findings suggest that miR-9 may be a potential target for modulating the radiosensitivity of NPC cells.
authors.affiliation: Department of Otorhinolaryngology, The First Affiliated Hospital and Otorhinolaryngology Institute, Sun Yat‑Sen University, Guangzhou, Guangdong 510080, P.R. China.
authors.forename: Chao-Pan
authors.initials: CP
authors.lastname: Zheng
authors.affiliation: Department of Otorhinolaryngology, The First Affiliated Hospital and Otorhinolaryngology Institute, Sun Yat‑Sen University, Guangzhou, Guangdong 510080, P.R. China.
authors.forename: Ling
authors.initials: L
authors.lastname: Han
authors.affiliation: Department of Otorhinolaryngology, The First Affiliated Hospital and Otorhinolaryngology Institute, Sun Yat‑Sen University, Guangzhou, Guangdong 510080, P.R. China.
authors.forename: Wei-Jian
authors.initials: WJ
authors.lastname: Hou
authors.affiliation: Department of Otolaryngology, First People's Hospital, Foshan, Guangdong 528000, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Tang
authors.affiliation: Department of Otorhinolaryngology, The First Affiliated Hospital and Otorhinolaryngology Institute, Sun Yat‑Sen University, Guangzhou, Guangdong 510080, P.R. China.
authors.forename: Yi-Hui
authors.initials: YH
authors.lastname: Wen
authors.affiliation: Department of Otorhinolaryngology, The First Affiliated Hospital and Otorhinolaryngology Institute, Sun Yat‑Sen University, Guangzhou, Guangdong 510080, P.R. China.
authors.forename: Ran
authors.initials: R
authors.lastname: Fu
authors.affiliation: Department of Otolaryngology, First People's Hospital, Foshan, Guangdong 528000, P.R. China.
authors.forename: Yue-Jian
authors.initials: YJ
authors.lastname: Wang
authors.affiliation: Department of Otorhinolaryngology, The First Affiliated Hospital and Otorhinolaryngology Institute, Sun Yat‑Sen University, Guangzhou, Guangdong 510080, P.R. China.
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Wen
mesh_terms: D017209:Apoptosis; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000528:radiation effects; D004249:DNA Damage; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D015972:Gene Expression Regulation, Neoplastic; D005978:Glutathione / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006801:Humans; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D009305:Nasopharynx / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D011836:Radiation Tolerance / Q000235:genetics*; D014466:Ultraviolet Rays
chemical_list: D015820:Cadherins; C541874:MIRN92 microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering; D005978:Glutathione