title: A spiroketal-enol ether derivative from Tanacetum vulgare selectively inhibits HSV-1 and HSV-2 glycoprotein accumulation in Vero cells.
abstract: The inhibitory effects of Tanacetum vulgare rhizome extracts on HSV-1 and HSV-2 in vitro replication were assessed. Unlike extracts obtained from the aerial parts, adsorption inhibition and virucidal activities seemed not to be relevant for the observed antiviral action of tansy rhizome extracts. Instead, the most significant effects were the inhibition of virus penetration and a novel mechanism consisting of the specific arrest of viral gene expression and consequently the decrease of viral protein accumulation within infected cells. Through a bioactivity-guided fractionation protocol we isolated and identified the spiroketal-enol ether derivative (E)-2-(2,4-hexadiynyliden)-1,6-dioxaspiro[4.5]dec-3-ene as the active compound responsible for this inhibitory effect.
authors.affiliation: Instituto Universitario de Biotecnología de Asturias, Departamento de Bioquímica y Biología Molecular, Universidad de Oviedo, 33006 Oviedo, Asturias, Spain. Electronic address: alvarezrangel@uniovi.es.
authors.forename: Ángel L
authors.initials: ÁL
authors.lastname: Álvarez
authors.affiliation: Pharmacognosy Research Laboratories, Medway School of Science, University of Greenwich, Central Avenue, Chatham-Maritime, Kent ME4 4TB, UK.
authors.forename: Solomon
authors.initials: S
authors.lastname: Habtemariam
authors.affiliation: Instituto Universitario de Biotecnología de Asturias, Departamento de Bioquímica y Biología Molecular, Universidad de Oviedo, 33006 Oviedo, Asturias, Spain.
authors.forename: Ahmed E
authors.initials: AE
authors.lastname: Abdel Moneim
authors.affiliation: Servicio de Microbiología, Laboratorio de Virología, Hospital Universitario Central de Asturias, Oviedo, Spain.
authors.forename: Santiago
authors.initials: S
authors.lastname: Melón
authors.affiliation: Instituto Universitario de Biotecnología de Asturias, Departamento de Bioquímica y Biología Molecular, Universidad de Oviedo, 33006 Oviedo, Asturias, Spain.
authors.forename: Kevin P
authors.initials: KP
authors.lastname: Dalton
authors.affiliation: Instituto Universitario de Biotecnología de Asturias, Departamento de Bioquímica y Biología Molecular, Universidad de Oviedo, 33006 Oviedo, Asturias, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Parra
mesh_terms: D000480:Alkynes / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D002522:Chlorocebus aethiops; D004986:Ether; D018259:Herpesvirus 1, Human / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D018258:Herpesvirus 2, Human / Q000187:drug effects* / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology; D027343:Rhizome / Q000737:chemistry; D013141:Spiro Compounds / Q000302:isolation & purification / Q000494:pharmacology*; D031203:Tanacetum / Q000737:chemistry*; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000378:metabolism*; D014779:Virus Replication / Q000187:drug effects / Q000235:genetics
chemical_list: C000604948:2-(2,4-hexadiynylidene)-1,6-dioxaspiro(4.5)dec-3-ene; D000480:Alkynes; D010936:Plant Extracts; D013141:Spiro Compounds; D014759:Viral Envelope Proteins; C045464:glycoprotein G, herpes simplex virus type 2; C038535:glycoprotein gC, herpes simplex virus type 1; D004986:Ether
keywords: Antiviral; HSV; Rhizome; Spiroketals; Tanacetum; Tansy