title: Prediction of host - pathogen protein interactions between Mycobacterium tuberculosis and Homo sapiens using sequence motifs.
abstract: BACKGROUND
  Emergence of multiple drug resistant strains of M. tuberculosis (MDR-TB) threatens to derail global efforts aimed at reigning in the pathogen. Co-infections of M. tuberculosis with HIV are difficult to treat. To counter these new challenges, it is essential to study the interactions between M. tuberculosis and the host to learn how these bacteria cause disease.
  
  
  RESULTS
  We report a systematic flow to predict the host pathogen interactions (HPIs) between M. tuberculosis and Homo sapiens based on sequence motifs. First, protein sequences were used as initial input for identifying the HPIs by 'interolog' method. HPIs were further filtered by prediction of domain-domain interactions (DDIs). Functional annotations of protein and publicly available experimental results were applied to filter the remaining HPIs. Using such a strategy, 118 pairs of HPIs were identified, which involve 43 proteins from M. tuberculosis and 48 proteins from Homo sapiens. A biological interaction network between M. tuberculosis and Homo sapiens was then constructed using the predicted inter- and intra-species interactions based on the 118 pairs of HPIs. Finally, a web accessible database named PATH (Protein interactions of M. tuberculosis and Human) was constructed to store these predicted interactions and proteins.
  
  
  CONCLUSIONS
  This interaction network will facilitate the research on host-pathogen protein-protein interactions, and may throw light on how M. tuberculosis interacts with its host.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, 300071, China. herohonan@126.com.|College of Life Sciences, Nankai University, Tianjin, 300071, China. herohonan@126.com.|Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin, 300457, China. herohonan@126.com.
authors.forename: Tong
authors.initials: T
authors.lastname: Huo
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, 300071, China. 595643971@qq.com.|College of Life Sciences, Nankai University, Tianjin, 300071, China. 595643971@qq.com.|Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin, 300457, China. 595643971@qq.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, 300071, China. yu.guo@htmdc.org.|College of Pharmacy, Nankai University, Tianjin, 300071, China. yu.guo@htmdc.org.|Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin, 300457, China. yu.guo@htmdc.org.
authors.forename: Yu
authors.initials: Y
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, 300071, China. cheng.yang@nankai.edu.cn.|College of Pharmacy, Nankai University, Tianjin, 300071, China. cheng.yang@nankai.edu.cn.|Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin, 300457, China. cheng.yang@nankai.edu.cn.
authors.forename: Cheng
authors.initials: C
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, 300071, China. jianpinglin@nankai.edu.cn.|College of Pharmacy, Nankai University, Tianjin, 300071, China. jianpinglin@nankai.edu.cn.|Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin, 300457, China. jianpinglin@nankai.edu.cn.
authors.forename: Jianping
authors.initials: J
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, 300071, China. raozh@nankai.edu.cn.|College of Life Sciences, Nankai University, Tianjin, 300071, China. raozh@nankai.edu.cn.|Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin, 300457, China. raozh@nankai.edu.cn.
authors.forename: Zihe
authors.initials: Z
authors.lastname: Rao
mesh_terms: D019295:Computational Biology / Q000379:methods*; D054884:Host-Pathogen Interactions; D006801:Humans; D058977:Molecular Sequence Annotation; D009169:Mycobacterium tuberculosis / Q000378:metabolism*; D025941:Protein Interaction Mapping / Q000379:methods*; D014376:Tuberculosis / Q000378:metabolism* / Q000382:microbiology