title: Self-powered competitive immunosensor driven by biofuel cell based on hollow-channel paper analytical devices.
abstract: A mediator-less and compartment-less glucose/O2 enzymatic biofuel cell (BFC) was introduced into microfluidic paper-based analytical devices (μ-PADs) that relies on flow in hollow channels with silver nanoparticles/graphene modified paper electrode as the anodic and cathodic substrate, to implement self-powered sensitive carcinoembryonic antigen (CEA) detection. Glucose dehydrogenase (GDH)-gold nanoparticles bioconjugate modified with CEA acted as a biocatalyst for enhancing glucose oxidation in the bioanode, as well as the transducing enzyme for signaling magnification. Similarly, nanoporous PtNi/bilirubin oxidase (BOD) acted as a biocatalyst for enhancing O2 reduction in the biocathode. With an increase in the concentration of CEA, the amount of CEA-Au-GDH bioconjugate on bioanode decreases, thus leading to the lower output of the as-prepared BFC. This proposed BFC-based self-powered immunosensor for CEA possessed largely increased linear detection range from 1 pg mL(-1) to 0.5 μg mL(-)(1) with a detection limit of 0.7 pg mL(-)(1). The proposed BFC-based self-powered immunosensor shows high sensitivity, stability, and reproducibility and can become a promising platform for other protein detection.
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Li
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Yanhu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Shandong Provincial Key Laboratory of Preparation and Measurement of Building Materials, School of Material Science and Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Shenguang
authors.initials: S
authors.lastname: Ge
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China. Electronic address: ujn.yujh@gmail.com.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yu
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Mei
authors.initials: M
authors.lastname: Yan
mesh_terms: D001674:Bioelectric Energy Sources; D015374:Biosensing Techniques / Q000295:instrumentation; D002272:Carcinoembryonic Antigen / Q000097:blood*; D055664:Electrochemical Techniques / Q000295:instrumentation; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000378:metabolism; D004867:Equipment Design; D005947:Glucose / Q000378:metabolism; D042843:Glucose 1-Dehydrogenase / Q000378:metabolism; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D056656:Lab-On-A-Chip Devices; D057230:Limit of Detection; D008958:Models, Molecular; D058608:Nanopores / Q000648:ultrastructure; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D009532:Nickel / Q000737:chemistry; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D010209:Paper; D010984:Platinum / Q000737:chemistry; D015203:Reproducibility of Results
chemical_list: D002272:Carcinoembryonic Antigen; D004800:Enzymes, Immobilized; D010984:Platinum; D009532:Nickel; D042843:Glucose 1-Dehydrogenase; D044925:Oxidoreductases Acting on CH-CH Group Donors; C042401:bilirubin oxidase; D005947:Glucose; D010100:Oxygen
keywords: Bilirubin oxidase; Biofuel cell; Glucose dehydrogenase; Microfluidic paper-based analytical devices; Nanoporous PtNi