title: Barosensitivity in Saccharomyces cerevisiae is Closely Associated with a Deletion of the COX1 Gene.
abstract: High hydrostatic pressure causes physical stress to microorganisms; therefore, this technology may be applied to food pasteurization without introducing the unfavorable effects of thermal denaturation. However, its application is limited to high-value foods because the treatment requires a robust steel vessel and expensive pressurization equipment. To reduce these costs, we studied the pasteurization of Saccharomyces cerevisiae using relatively moderate high-pressure levels. A mutant strain isolated by ultraviolet mutagenesis showed significant loss of viability under high-pressure conditions. Gene expression analysis of the mutant strain revealed that it incurred a deletion of the COX1 gene. Our results suggest that the pressure-sensitivity can readily be introduced into industrial/food microorganisms by complementing a COX1 deleted mitochondria.
authors.affiliation: The United Graduate School of Agricultural Science, Gifu Univ., 1-1 Yanagido, Gifu-shi, Gifu, 501-1193, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Nomura
authors.affiliation: The United Graduate School of Agricultural Science, Gifu Univ., 1-1 Yanagido, Gifu-shi, Gifu, 501-1193, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Iwahashi
authors.affiliation: Dept. of Food Science, Faculty of Applied Life Sciences, Niigata Univ. of Pharmacy and Applied Life Sciences (NUPALS), 265-1 Higashijima, Akiha-ku, Niigata-shi, Niigata, 956-8603, Japan.
authors.forename: Akinori
authors.initials: A
authors.lastname: Iguchi
authors.affiliation: Dept. of Food Science, Faculty of Applied Life Sciences, Niigata Univ. of Pharmacy and Applied Life Sciences (NUPALS), 265-1 Higashijima, Akiha-ku, Niigata-shi, Niigata, 956-8603, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Shigematsu
mesh_terms: D051545:Cyclooxygenase 1 / Q000235:genetics*; D017353:Gene Deletion; D006874:Hydrostatic Pressure; D008928:Mitochondria / Q000201:enzymology; D016296:Mutagenesis; D061352:Pasteurization / Q000295:instrumentation; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics / Q000502:physiology*
chemical_list: D051545:Cyclooxygenase 1
keywords: DNA microarray; Saccharomyces cerevisiae; high-pressure processing; mitochondria; pressure-regulated fermentation