title: Effect of Puerarin on the Pharmacokinetics of Baicalin in Gegen Qinlian Decoction () in Mice.
abstract: OBJECTIVE
  To study the pharmacokinetics of puerarin (PUE) in Gegen Qinlian Decoction (, GQD), and the effects of PUE dosage variations on the pharmacokinetics of baicalin (BAL) in mice.
  
  
  METHODS
  GQD is composed of the concentrated granules of four Chinese herbs. Three dosages with different levels of PUE, including GQD, GQD co-administered with PUE, and GQD co-administration with two times the amount of PUE, were used to research the pharmacokinetics of PUE and BAL in mice. The indirect competitive enzyme-linked immunosorbent assay (icELISA) methods based on an anti PUE-monoclonal antibody (MAb)and BAL-MAb were employed to determine the concentration of PUE and BAL in mice blood.
  
  
  RESULTS
  After the co-administration of GQD with PUE, the area under the curves (AUC0-14h) of PUE increased 2.8 times compared with GQD. At the dose of GQD co-administration at two times that of PUE, the (AUC0-14h) of PUE was almost equal to that of GQD co-administration of PUE, showing non-linear pharmacokinetics. The (AUC0-48h) of BAL showed a good dose-related increase of PUE (r=0.993) in the range from 100 to 300 mg/kg, indicating that PUE dramatically affects the absorption of BAL in mice. There was no significant difference in the other pharmacokinetic parameters, such as the first time of maximum concentration (Tmax), the second Tmax, or the mean residence time.
  
  
  CONCLUSIONS
  The icELISA methods were successfully applied to pharmacokinetic studies of PUE and BAL in GQD in mice. The dosage variability of PUE of the main ingredient in GQD affects its own pharmacokinetic characteristics and the absorption characteristics of BAL.
authors.affiliation: School of Preclinical Medicine, Beijing University of Chinese Medicine, 100029, Beijing, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Kong
authors.affiliation: School of Preclinical Medicine, Beijing University of Chinese Medicine, 100029, Beijing, China.
authors.forename: Xue-Qian
authors.initials: XQ
authors.lastname: Wang
authors.affiliation: School of Preclinical Medicine, Beijing University of Chinese Medicine, 100029, Beijing, China.
authors.forename: Qing-Guo
authors.initials: QG
authors.lastname: Wang
authors.affiliation: School of Preclinical Medicine, Beijing University of Chinese Medicine, 100029, Beijing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: School of Preclinical Medicine, Beijing University of Chinese Medicine, 100029, Beijing, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Sun
authors.affiliation: School of Preclinical Medicine, Beijing University of Chinese Medicine, 100029, Beijing, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Preclinical Medicine, Beijing University of Chinese Medicine, 100029, Beijing, China.
authors.forename: Jie-Kun
authors.initials: JK
authors.lastname: Xu
authors.affiliation: Center of Scientific Experiment, Beijing University of Chinese Medicine, 100029, Beijing, China. quhuihua@gmail.com.
authors.forename: Hui-Hua
authors.initials: HH
authors.lastname: Qu
mesh_terms: D000818:Animals; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics*; D004797:Enzyme-Linked Immunosorbent Assay; D005419:Flavonoids / Q000493:pharmacokinetics*; D041743:Herb-Drug Interactions; D007529:Isoflavones / Q000494:pharmacology*; D008297:Male; D051379:Mice; D014665:Vasodilator Agents / Q000494:pharmacology*
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D007529:Isoflavones; D014665:Vasodilator Agents; C542794:gegenqinlian; C038044:baicalin; C033607:puerarin
keywords: Chinese medicine; Gegen Qinlian Decoction; baicalin; indirect competitive enzyme-linked immunosorbent assay; pharmacokinetics; puerarin