title: Gelatin for purification and proliferation of primary keratinocyte culture for use in chronic wounds and burns.
abstract: OBJECTIVE
  Human epidermal keratinocytes are currently established as a treatment for burns and wounds and have laboratory applications. Keratinocyte culture contamination by unwanted cells and inhibition of cell proliferation are barriers in primary keratinocyte culture. According to the recent literature, these cells are hard to culture. The present study was conducted to evaluate the efficacy of gelatin-coated surfaces in keratinocyte cultures.
  
  
  METHODS
  After enzymatic isolation of keratinocytes from normal epidermis by trypsin, the cells were cultured on gelatin-coated flasks in serum-free medium. Another group of cells were cultured as a control group without gelatin coating.
  
  
  RESULTS
  We showed positive effects of surface coating with gelatin on the primary culture of keratinocytes. Culture of these cells on a gelatincoated surface showed better proliferation with suitable morphology. By using gelatin, adhesion of these cells to the surface was more efficient and without contamination by small round cells.
  
  
  CONCLUSIONS
  Successful primary culture of keratinocytes on a gelatin-coated surface may provide better yield and optimal number of cells for research and clinical applications.
authors.affiliation: From the Shiraz Transplant Research Center, Shiraz, Iran.
authors.forename: Marjan
authors.initials: M
authors.lastname: Rahsaz
authors.forename: Bita
authors.initials: B
authors.lastname: Geramizadeh
authors.forename: Maryam
authors.initials: M
authors.lastname: Kaviani
authors.forename: Saeed
authors.initials: S
authors.lastname: Marzban
mesh_terms: D002056:Burns / Q000473:pathology / Q000601:surgery*; D002448:Cell Adhesion; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods*; D048430:Cell Shape; D002478:Cells, Cultured; D005780:Gelatin / Q000378:metabolism*; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism* / Q000637:transplantation; D061251:Primary Cell Culture; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014945:Wound Healing
chemical_list: D005780:Gelatin