title: [The tick-borne encephalitis virus detection efficiency in the Ixodid ticks (Acari: Ixodidae) with ELISA and real-time PCR].
abstract: According to the data of the Federal Service on Customers' Rights Protection and Human Well-being Surveillance, the enzyme-linked immunosorbent assay (ELISA) detects the tick-borne encephalitis virus (TBEV) in ticks more often than the polymerase chain reaction (PCR). The goal of this work was to compare TBEV detection efficiency in the ixodid ticks of different species with the commercial kits based on ELISA and real-time PCR. Ticks of five species were parenterally infected with 2-6 IgPFU of the European or Siberian TBEV subtypes. We formed randomized and encoded series of infected and intact ticks of different species, and in "blind" experiment analyzed the ticks on the TBEV presence with the kits based on ELISA and real-time PCR. ELISA and real-time PCR effectiveness of the TBEV detection in ticks was not affected by gender, species of ticks or presence of blood meal. The kits based on ELISA were less sensitive than those based on real-time PCR. ELISA effectiveness depended on the TBEV subtype. The presence of the false positive reactions and sensitivity of ELISA were affected by the protocols of reaction. The problem of the different TBEV prevalence in the field-collected ticks obtained with various methods remains to be studied.
authors.forename: O A
authors.initials: OA
authors.lastname: Belova
authors.forename: L S
authors.initials: LS
authors.lastname: Karan'
authors.forename: N M
authors.initials: NM
authors.lastname: Koliasnikova
authors.forename: N G
authors.initials: NG
authors.lastname: Topychkanova
authors.forename: I N
authors.initials: IN
authors.lastname: Kuvshinova
authors.forename: D I
authors.initials: DI
authors.lastname: Timofeev
authors.forename: M Iu
authors.initials: MIu
authors.lastname: Rukavishnikov
authors.forename: M P
authors.initials: MP
authors.lastname: Grishaev
authors.forename: G G
authors.initials: GG
authors.lastname: Karganova
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D004669:Encephalitis Viruses, Tick-Borne / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards*; D005189:False Positive Reactions; D005260:Female; D026863:Ixodidae / Q000821:virology*; D008297:Male; D051379:Mice; D012367:RNA, Viral / Q000235:genetics*; D011817:Rabbits; D060888:Real-Time Polymerase Chain Reaction / Q000592:standards*; D012680:Sensitivity and Specificity; D019562:Viral Load
chemical_list: D000956:Antigens, Viral; D012367:RNA, Viral