title: Characterization and real-time imaging of the FTLD-related protein aggregation induced by amyloidogenic peptides.
abstract: We identify a new amyloidogenic peptide from the glutamine/asparagine-rich region of the FTLD-related protein (TDP-43), which can seed both the full-length and N-terminus-truncated TDP-43. Through the microinjection and real-time fluorescence imaging, we also found that this novel peptide could trigger cell apoptosis and initiate TDP-43 aggregation in the cytosol.
authors.affiliation: Institute of Chemistry, Academia Sinica, No. 128, Sec. 2, Academia Road, Nankang, Taipei 115, Taiwan. jthuang@gate.sinica.edu.tw leehm@chem.sinica.edu.tw.
authors.forename: Ruei-Yu
authors.initials: RY
authors.lastname: He
authors.forename: Yi-Chen
authors.initials: YC
authors.lastname: Huang
authors.forename: Chao-Wei
authors.initials: CW
authors.lastname: Chiang
authors.forename: Yu-Ju
authors.initials: YJ
authors.lastname: Tsai
authors.forename: Ting-Juan
authors.initials: TJ
authors.lastname: Ye
authors.forename: Hua-De
authors.initials: HD
authors.lastname: Gao
authors.forename: Chu-Ya
authors.initials: CY
authors.lastname: Wu
authors.forename: Hsien-Ming
authors.initials: HM
authors.lastname: Lee
authors.forename: Joseph Jen-Tse
authors.initials: JJ
authors.lastname: Huang
mesh_terms: D058227:Amyloidogenic Proteins / Q000737:chemistry* / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002461:Cell Line, Transformed; D003600:Cytosol / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D057174:Frontotemporal Lobar Degeneration; D006801:Humans; D015394:Molecular Structure; D066329:Protein Aggregates / Q000187:drug effects*; D066263:Protein Aggregation, Pathological / Q000139:chemically induced*; D051381:Rats; D013050:Spectrometry, Fluorescence; D013997:Time Factors
chemical_list: D058227:Amyloidogenic Proteins; D004268:DNA-Binding Proteins; D005456:Fluorescent Dyes; D066329:Protein Aggregates; C000598624:TARDBP protein, human