title: Phylogeny and evolution of plant macrophage migration inhibitory factor/D-dopachrome tautomerase-like proteins.
abstract: BACKGROUND
  The human (Homo sapiens) chemokine-like protein macrophage migration inhibitory factor (HsMIF) is a pivotal mediator of inflammatory, infectious and immune diseases including septic shock, colitis, malaria, rheumatoid arthritis, and atherosclerosis, as well as tumorigenesis. HsMIF has been found to exhibit several sequential and three-dimensional sequence motifs that in addition to its receptor binding sites include catalytic sites for oxidoreductase and tautomerase activity, which provide this 12.5 kDa protein with a remarkable functional complexity. A human MIF paralog, D-dopachrome tautomerase (HsDDT), has been identified, but its physiological relevance is incompletely understood. MIF/DDT-like proteins have been described in animals, protists and bacteria. Although based on sequence data banks the presence of MIF/DDT-like proteins has also been recognized in the model plant species Arabidopsis thaliana, details on these plant proteins have not been reported.
  
  
  RESULTS
  To broaden the understanding of the biological role of these proteins across kingdoms we performed a comprehensive in silico analysis of plant MIF/DDT-like (MDL) genes/proteins. We found that the A. thaliana genome harbors three MDL genes, of which two are chiefly constitutively expressed in aerial plant organs, while the third gene shows stress-inducible transcript accumulation. The product of the latter gene likely localizes to peroxisomes. Structure prediction suggests that all three Arabidopsis proteins resemble the secondary and tertiary structure of human MIF. MIF-like proteins are found in all species across the plant kingdom, with an increasing family complexity towards evolutionarily advanced plant taxa. Plant MDL proteins are predicted to lack oxidoreductase activity, but possibly share tautomerase activity with human MIF/DDT.
  
  
  CONCLUSIONS
  Peroxisome localization seems to be a specific feature of a subset of MIF/DDT orthologs found in dicotyledonous plant species, which together with its stress-inducible gene expression might point to convergent evolution in higher plants and vertebrates towards neofunctionalization of MIF/MDL proteins in stress response pathways including innate immunity.
authors.affiliation: RWTH Aachen University, Institute of Biology I, Unit of Plant Molecular Cell Biology, Worringerweg 1, 52074, Aachen, Germany. panstruga@bio1.rwth-aachen.de.
authors.forename: Ralph
authors.initials: R
authors.lastname: Panstruga
authors.affiliation: RWTH Aachen University, Institute of Biology I, Unit of Plant Molecular Cell Biology, Worringerweg 1, 52074, Aachen, Germany. kira.baumgarten@rwth-aachen.de.
authors.forename: Kira
authors.initials: K
authors.lastname: Baumgarten
authors.affiliation: RWTH Aachen University, Institute of Biochemistry and Molecular Cell Biology, Pauwelsstrasse 30, 52074, Aachen, Germany. jbernhagen@ukaachen.de.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Bernhagen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000235:genetics; D019143:Evolution, Molecular; D006801:Humans; D019746:Intramolecular Oxidoreductases / Q000737:chemistry / Q000235:genetics*; D008263:Macrophage Migration-Inhibitory Factors / Q000737:chemistry / Q000235:genetics*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment
chemical_list: D008263:Macrophage Migration-Inhibitory Factors; D010940:Plant Proteins; D019746:Intramolecular Oxidoreductases; C042501:dopachrome isomerase