title: CalFluors: A Universal Motif for Fluorogenic Azide Probes across the Visible Spectrum.
abstract: Fluorescent bioorthogonal smart probes across the visible spectrum will enable sensitive visualization of metabolically labeled molecules in biological systems. Here we present a unified design, based on the principle of photoinduced electron transfer, to access a panel of highly fluorogenic azide probes that are activated by conversion to the corresponding triazoles via click chemistry. Termed the CalFluors, these probes possess emission maxima that range from green to far red wavelengths, and enable sensitive biomolecule detection under no-wash conditions. We used the CalFluor probes to image various alkyne-labeled biomolecules (glycans, DNA, RNA, and proteins) in cells, developing zebrafish, and mouse brain tissue slices.
authors.forename: Peyton
authors.initials: P
authors.lastname: Shieh
authors.forename: Vivian T
authors.initials: VT
authors.lastname: Dien
authors.forename: Brendan J
authors.initials: BJ
authors.lastname: Beahm
authors.affiliation: ∥Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, California 94305, United States.
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Castellano
authors.affiliation: ∥Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, California 94305, United States.
authors.forename: Tony
authors.initials: T
authors.lastname: Wyss-Coray
authors.forename: Carolyn R
authors.initials: CR
authors.lastname: Bertozzi
mesh_terms: D000818:Animals; D001386:Azides / Q000737:chemistry*; D004247:DNA / Q000032:analysis; D051379:Mice; D015335:Molecular Probes; D011134:Polysaccharides / Q000032:analysis; D011506:Proteins / Q000032:analysis; D012313:RNA / Q000032:analysis; D015027:Zebrafish
chemical_list: D001386:Azides; D015335:Molecular Probes; D011134:Polysaccharides; D011506:Proteins; D012313:RNA; D004247:DNA