title: High expression of neuroguidin increases the sensitivity of acute myeloid leukemia cells to chemotherapeutic drugs.
abstract: Neuroguidin (NGDN) is a eukaryotic translation initiation factor 4E binding protein. The purpose of this study was to clarify the function of NGDN and its possible mechanism of action in human myeloid leukemia cells. Proliferation inhibition and apoptosis in NGDN over-expressing myeloid multidrug-resistant leukemia cells (K562/A02-NGDN) was significantly higher than in control K562/A02 cells following treatment with vincristine, etoposide, and epirubicin, indicating that NGDN over-expression can increase the sensitivity of multidrug-resistant leukemia cells to chemotherapeutic drugs. Furthermore, NGDN knock-down in K562/A02 cells resulted in the activation of multiple tumor-related signaling pathways, especially the mammalian target of rapamycin (mTOR) pathway.
authors.affiliation: Department of Hematology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai, 200433, China. ckjzl13817210306@163.com.
authors.forename: Kejun
authors.initials: K
authors.lastname: Chen
authors.affiliation: Department of Hematology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai, 200433, China. shuqing7266@163.com.
authors.forename: Shuqing
authors.initials: S
authors.lastname: Lü
authors.affiliation: Department of Hematology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai, 200433, China. chenghui19831103@163.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Cheng
authors.affiliation: Department of Hematology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai, 200433, China. drake015@163.com.
authors.forename: Gusheng
authors.initials: G
authors.lastname: Tang
authors.affiliation: Department of Hematology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai, 200433, China. 443089413@qq.com.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Hematology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai, 200433, China. shilehua100@163.com.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Department of Hematology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai, 200433, China. jmwang@medmail.com.cn.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Wang
mesh_terms: D017209:Apoptosis / Q000502:physiology; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm / Q000502:physiology*; D005434:Flow Cytometry; D055785:Gene Knockdown Techniques; D006801:Humans; D020014:K562 Cells; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism*; D009687:Nuclear Proteins / Q000096:biosynthesis*; D016601:RNA-Binding Proteins
chemical_list: C532399:NGDN protein, human; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins