title: Expression, purification and characterization of cold shock protein A of Corynebacterium pseudotuberculosis.
abstract: The gram-positive bacterium Corynebacterium pseudotuberculosis is the causative agent of different diseases that cause dramatically reduced yields of wool and milk, and results in weight loss, carcass condemnation and also death mainly in sheep, equids, cattle and goats and therefore globally results in considerable economical loss. Cold shock proteins are conserved in many bacteria and eukaryotic cells and they help to restore normal cell functions after cold shock in which some appear to have specific functions at normal growth temperature as well. Cold shock protein A from C. pseudotuberculosis was expressed in Escherichia coli and purified. The thermal unfolding/refolding process characterized by circular dichroism, differential scanning calorimetry and NMR spectroscopy techniques indicated that the refolding process was almost completely reversible.
authors.affiliation: Multiuser Center for Biomolecular Innovation, Departament of Physics, Universidade Estadual Paulista (UNESP), São Jose do Rio Preto-SP 15054-000, Brazil.
authors.forename: Antje
authors.initials: A
authors.lastname: Lindae
authors.affiliation: Multiuser Center for Biomolecular Innovation, Departament of Physics, Universidade Estadual Paulista (UNESP), São Jose do Rio Preto-SP 15054-000, Brazil.
authors.forename: Raphael J
authors.initials: RJ
authors.lastname: Eberle
authors.affiliation: Multiuser Center for Biomolecular Innovation, Departament of Physics, Universidade Estadual Paulista (UNESP), São Jose do Rio Preto-SP 15054-000, Brazil.
authors.forename: Icaro P
authors.initials: IP
authors.lastname: Caruso
authors.affiliation: Multiuser Center for Biomolecular Innovation, Departament of Physics, Universidade Estadual Paulista (UNESP), São Jose do Rio Preto-SP 15054-000, Brazil.
authors.forename: Monika A
authors.initials: MA
authors.lastname: Coronado
authors.affiliation: Multiuser Center for Biomolecular Innovation, Departament of Physics, Universidade Estadual Paulista (UNESP), São Jose do Rio Preto-SP 15054-000, Brazil.
authors.forename: Fabio R
authors.initials: FR
authors.lastname: de Moraes
authors.affiliation: Laboratório de Genética Celular e Molecular, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av, Antônio Carlos, 6627 - Pampulha, CP 486, CEP 31, Belo Horizonte, MG 270-901, Brazil.
authors.forename: Vasco
authors.initials: V
authors.lastname: Azevedo
authors.affiliation: Multiuser Center for Biomolecular Innovation, Departament of Physics, Universidade Estadual Paulista (UNESP), São Jose do Rio Preto-SP 15054-000, Brazil. Electronic address: arni@sjrp.unesp.br.
authors.forename: Raghuvir K
authors.initials: RK
authors.lastname: Arni
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D003001:Cloning, Molecular; D057773:Cold Shock Proteins and Peptides / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D003354:Corynebacterium Infections / Q000382:microbiology / Q000662:veterinary; D016925:Corynebacterium pseudotuberculosis / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D006041:Goats; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D011489:Protein Denaturation; D058849:Protein Refolding; D017433:Protein Structure, Secondary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D016415:Sequence Alignment; D012756:Sheep; D044366:Transition Temperature
chemical_list: D057773:Cold Shock Proteins and Peptides; D011993:Recombinant Fusion Proteins
keywords: C. pseudotuberculosis; Cold shock protein; Folding and refolding proteins; Secondary structure; Spectroscopic methods