title: A simple U-HPLC-MS/MS method for the determination of liensinine and isoliensinine in rat plasma.
abstract: An ultra-high performance liquid chromatography tandem mass spectrometry (U-HPLC-MS/MS) method was developed and validated to determine liensinine and isoliensinine in rat plasma simultaneously. Plasma samples were prepared using protein precipitation with acetonitrile. The two analytes and the internal standard pirfenidone were separated on an Acquity U-HPLC BEH C18 column with the mobile phase of acetonitrile and 1% formic acid in water with gradient elution at a flow rate of 0.40mL/min. Both liensinine and isoliensinine were eluted at 0.63 and 0.82min, respectively. The detection was performed on a triple quadrupole tandem mass spectrometer equipped with positive-ion electrospray ionization (ESI) by multiple reactions monitoring (MRM) of the transitions at m/z 611.6 → 206.2 for liensinine and m/z 611.4 → 192.2 for isoliensinine. The linearity of this method was found to be within the concentration range of 5-700ng/mL for liensinine and isoliensinine in rat plasma. The lower limits of quantification (LLOQ) were all 5ng/mL for liensinine and isoliensinine. The relative standard deviations (RSD) of intra and inter precision were less than 10% for both liensinine and isoliensinine. The method was also successfully applied to the pharmacokinetic study of liensinine and isoliensinine in rats.
authors.affiliation: Shenzhen TCM Hospital Affiliated to Guangzhou University of Traditional Chinese Medicine, Shenzhen 518033, China.
authors.forename: Li-Sheng
authors.initials: LS
authors.lastname: Peng
authors.affiliation: Shenzhen TCM Hospital Affiliated to Guangzhou University of Traditional Chinese Medicine, Shenzhen 518033, China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Jiang
authors.affiliation: Shenzhen TCM Hospital Affiliated to Guangzhou University of Traditional Chinese Medicine, Shenzhen 518033, China.
authors.forename: Zhong-Xin
authors.initials: ZX
authors.lastname: Li
authors.affiliation: Shenzhen TCM Hospital Affiliated to Guangzhou University of Traditional Chinese Medicine, Shenzhen 518033, China.
authors.forename: Tie-Gang
authors.initials: TG
authors.lastname: Yi
authors.affiliation: Shenzhen TCM Hospital Affiliated to Guangzhou University of Traditional Chinese Medicine, Shenzhen 518033, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Huang
authors.affiliation: Shenzhen TCM Hospital Affiliated to Guangzhou University of Traditional Chinese Medicine, Shenzhen 518033, China.
authors.forename: Hui-Lin
authors.initials: HL
authors.lastname: Li
authors.affiliation: Shenzhen TCM Hospital Affiliated to Guangzhou University of Traditional Chinese Medicine, Shenzhen 518033, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zeng
authors.affiliation: Shenzhen TCM Hospital Affiliated to Guangzhou University of Traditional Chinese Medicine, Shenzhen 518033, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Shenzhen TCM Hospital Affiliated to Guangzhou University of Traditional Chinese Medicine, Shenzhen 518033, China.
authors.forename: Song-Ling
authors.initials: SL
authors.lastname: Peng
authors.affiliation: Shenzhen TCM Hospital Affiliated to Guangzhou University of Traditional Chinese Medicine, Shenzhen 518033, China.
authors.forename: Jin-Song
authors.initials: JS
authors.lastname: He
authors.affiliation: Shenzhen TCM Hospital Affiliated to Guangzhou University of Traditional Chinese Medicine, Shenzhen 518033, China.
authors.forename: Li
authors.initials: L
authors.lastname: He
authors.affiliation: Shenzhen TCM Hospital Affiliated to Guangzhou University of Traditional Chinese Medicine, Shenzhen 518033, China. Electronic address: plp001sz@163.com.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Peng
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007546:Isoquinolines / Q000097:blood*; D057230:Limit of Detection; D008297:Male; D010636:Phenols / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D007546:Isoquinolines; D010636:Phenols; C499779:isoliensinine; C080095:liensinine
keywords: Isoliensinine; Liensinine; Pharmacokinetic; Rat plasma; U-HPLC-MS/MS