title: Stretch calculated from grip distance accurately approximates mid-specimen stretch in large elastic arteries in uniaxial tensile tests.
abstract: The mechanical properties of vascular tissues affect hemodynamics and can alter disease progression. The uniaxial tensile test is a simple and effective method for determining the stress-strain relationship in arterial tissue ex vivo. To enable calculation of strain, stretch can be measured directly with image tracking of markers on the tissue or indirectly from the distance between the grips used to hold the specimen. While the imaging technique is generally considered more accurate, it also requires more analysis, and the grip distance method is more widely used. The purpose of this study is to compare the stretch of the testing specimen calculated from the grip distance method to that obtained from the imaging method for canine descending aortas and large proximal pulmonary arteries. Our results showed a significant difference in stretch between the two methods; however, this difference was consistently less than 2%. Therefore, the grip distance method is an accurate approximation of the stretch in large elastic arteries in the uniaxial tensile test.
authors.affiliation: Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI 53706-1609, USA.
authors.forename: Lian
authors.initials: L
authors.lastname: Tian
authors.affiliation: Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI 53706-1609, USA.
authors.forename: Joseph
authors.initials: J
authors.lastname: Henningsen
authors.affiliation: Department of Engineering Physics, University of Wisconsin-Madison, Madison, WI 53706-1609, USA.
authors.forename: Max R
authors.initials: MR
authors.lastname: Salick
authors.affiliation: Department of Engineering Physics, University of Wisconsin-Madison, Madison, WI 53706-1609, USA.
authors.forename: Wendy C
authors.initials: WC
authors.lastname: Crone
authors.affiliation: Department of Surgery, Division of Otolaryngology-Head and Neck Surgery, University of Wisconsin-Madison, Madison, WI 53792-3252, USA.
authors.forename: McLean
authors.initials: M
authors.lastname: Gunderson
authors.affiliation: Department of Surgery, Division of Otolaryngology-Head and Neck Surgery, University of Wisconsin-Madison, Madison, WI 53792-3252, USA.
authors.forename: Seth H
authors.initials: SH
authors.lastname: Dailey
authors.affiliation: Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI 53706-1609, USA. Electronic address: chesler@engr.wisc.edu.
authors.forename: Naomi C
authors.initials: NC
authors.lastname: Chesler
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic; D004285:Dogs; D004548:Elasticity; D008422:Materials Testing; D011651:Pulmonary Artery; D013314:Stress, Mechanical; D013718:Tensile Strength
keywords: Grip distance; Imaging technique; Stretch estimation; Uniaxial test