title: Decoding of quantum dots encoded microbeads using a hyperspectral fluorescence imaging method.
abstract: We presented a decoding method of quantum dots encoded microbeads with its fluorescence spectra using line scan hyperspectral fluorescence imaging (HFI) method. A HFI method was developed to attain both the spectra of fluorescence signal and the spatial information of the encoded microbeads. A decoding scheme was adopted to decode the spectra of multicolor microbeads acquired by the HFI system. Comparison experiments between the HFI system and the flow cytometer were conducted. The results showed that the HFI system has higher spectrum resolution; thus, more channels in spectral dimension can be used. The HFI system detection and decoding experiment with the single-stranded DNA (ssDNA) immobilized multicolor beads was done, and the result showed the efficiency of the HFI system. Surface modification of the microbeads by use of the polydopamine was characterized by the scanning electron microscopy and ssDNA immobilization was characterized by the laser confocal microscope. These results indicate that the designed HFI system can be applied to practical biological and medical applications.
authors.affiliation: ‡Department of Physics, Tsinghua University, Beijing 100084, China.
authors.forename: Yixi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: §Institute of Green Chemistry and Energy, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China.
authors.forename: Le
authors.initials: L
authors.lastname: Liu
authors.affiliation: ‡Department of Physics, Tsinghua University, Beijing 100084, China.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: He
authors.affiliation: ‡Department of Physics, Tsinghua University, Beijing 100084, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhu
authors.affiliation: ‡Department of Physics, Tsinghua University, Beijing 100084, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Ma
mesh_terms: D001483:Base Sequence; D004277:DNA, Single-Stranded / Q000032:analysis*; D004867:Equipment Design; D061925:Immobilized Nucleic Acids / Q000032:analysis*; D007211:Indoles / Q000737:chemistry; D018613:Microscopy, Confocal; D008863:Microspheres; D061848:Optical Imaging / Q000295:instrumentation*; D011108:Polymers / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D011145:Polyvinyls / Q000737:chemistry; D045663:Quantum Dots / Q000737:chemistry*
chemical_list: D004277:DNA, Single-Stranded; D061925:Immobilized Nucleic Acids; D007211:Indoles; D011108:Polymers; D011137:Polystyrenes; D011145:Polyvinyls; C442400:poly(chloromethylstyrene-co-divinylbenzene); C568283:polydopamine