title: Peptide based DNA nanocarriers incorporating a cell-penetrating peptide derived from neurturin protein and poly-L-lysine dendrons.
abstract: Multicomponent gene delivery systems incorporating cell-penetrating peptides (CPP) from the human neurturin protein (NRTN-30, NRTN(132-161); NRTN-17, NRTN(145-161)) and a poly-l-lysine (PLL) dendron, were synthesized and characterized for plasmid DNA (pDNA) delivery. Acetylated NRTN peptides (Ac-CPP) and peptides conjugated to a PLL dendron (DEN-CPP) efficiently condensed and stabilized pDNA. Complexes between pDNA and DEN-CPP formed smaller and more stable nanoparticles. Flow cytometry experiments showed that pDNA-DEN-CPPs were taken up more efficiently into HeLa cells. There was also no significant difference between NRTN-30 and NRTN-17 for pDNA uptake, indicating that the truncated peptide alone is sufficient as a CPP for pDNA delivery.
authors.affiliation: School of Chemistry and Molecular Biosciences, University of Queensland, St. Lucia 4072, Australia.
authors.forename: Nurlina
authors.initials: N
authors.lastname: Rosli
authors.affiliation: School of Chemistry and Molecular Biosciences, University of Queensland, St. Lucia 4072, Australia.
authors.forename: Michelle P
authors.initials: MP
authors.lastname: Christie
authors.affiliation: School of Pharmacy, Pharmacy Australia Centre of Excellence, University of Queensland, Woolloongabba 4102, Australia. Electronic address: p.moyle@uq.edu.au.
authors.forename: Peter M
authors.initials: PM
authors.lastname: Moyle
authors.affiliation: School of Chemistry and Molecular Biosciences, University of Queensland, St. Lucia 4072, Australia; School of Pharmacy, Pharmacy Australia Centre of Excellence, University of Queensland, Woolloongabba 4102, Australia. Electronic address: i.toth@uq.edu.au.
authors.forename: Istvan
authors.initials: I
authors.lastname: Toth
mesh_terms: D000595:Amino Acid Sequence; D001692:Biological Transport; D057846:Cell-Penetrating Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D050091:Dendrimers / Q000737:chemistry*; D018014:Gene Transfer Techniques; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D053758:Nanoparticles / Q000737:chemistry; D051101:Neurturin / Q000737:chemistry*; D010957:Plasmids / Q000737:chemistry; D011107:Polylysine / Q000737:chemistry*
chemical_list: D057846:Cell-Penetrating Peptides; D050091:Dendrimers; C495053:NRTN protein, human; D051101:Neurturin; D011107:Polylysine; D004247:DNA
keywords: Cell-penetrating peptides; DNA delivery; Gene therapy; Neurturin; Poly-l-lysine dendron