title: Different contribution of BRINP3 gene in chronic periodontitis and peri-implantitis: a cross-sectional study.
abstract: BACKGROUND
  Peri-implantitis is a chronic inflammation, resulting in loss of supporting bone around implants. Chronic periodontitis is a risk indicator for implant failure. Both diseases have a common etiology regarding inflammatory destructive response. BRINP3 gene is associated with aggressive periodontitis. However, is still unclear if chronic periodontitis and peri-implantitis have the same genetic background. The aim of this work was to investigate the association between BRINP3 genetic variation (rs1342913 and rs1935881) and expression and susceptibility to both diseases.
  
  
  METHODS
  Periodontal and peri-implant examinations were performed in 215 subjects, divided into: healthy (without chronic periodontitis and peri-implantitis, n = 93); diseased (with chronic periodontitis and peri-implantitis, n = 52); chronic periodontitis only (n = 36), and peri-implantitis only (n = 34). A replication sample of 92 subjects who lost implants and 185 subjects successfully treated with implants were tested. DNA was extracted from buccal cells. Two genetic markers of BRINP3 (rs1342913 and rs1935881) were genotyped using TaqMan chemistry. Chi-square (p < 0.05) compared genotype and allele frequency between groups. A subset of subjects (n = 31) had gingival biopsies harvested. The BRINP3 mRNA levels were studied by CT method (2(ΔΔCT)). Mann-Whitney test correlated the levels of BRINP3 in each group (p < 0.05).
  
  
  RESULTS
  Statistically significant association between BRINP3 rs1342913 and peri-implantitis was found in both studied groups (p = 0.04). The levels of BRINP3 mRNA were significantly higher in diseased subjects compared to healthy individuals (p = 0.01).
  
  
  CONCLUSIONS
  This study provides evidence that the BRINP3 polymorphic variant rs1342913 and low level of BRINP3 expression are associated with peri-implantitis, independently from the presence of chronic periodontitis.
authors.affiliation: Dental School, Clinical Research Unit and Biology Institute, Federal Fluminense University - UFF, Niterói, RJ, Brazil. plcasado@hotmail.com.|Center of Clinical Research, Orthopedics and Traumatology National Institute - INTO, Rio de Janeiro, RJ, Brazil. plcasado@hotmail.com.
authors.forename: Priscila L
authors.initials: PL
authors.lastname: Casado
authors.affiliation: Center of Clinical Research, Orthopedics and Traumatology National Institute - INTO, Rio de Janeiro, RJ, Brazil. diegopaguiar@gmail.com.
authors.forename: Diego P
authors.initials: DP
authors.lastname: Aguiar
authors.affiliation: Department of Dentistry, Federal Fluminense University - UFF, Niterói, RJ, Brazil. lucas.implante@gmail.com.
authors.forename: Lucas C
authors.initials: LC
authors.lastname: Costa
authors.affiliation: Department of Dentistry, Federal Fluminense University - UFF, Niterói, RJ, Brazil. mdentista.fonseca@gmail.com.
authors.forename: Marcos A
authors.initials: MA
authors.lastname: Fonseca
authors.affiliation: Center of Clinical Research, Orthopedics and Traumatology National Institute - INTO, Rio de Janeiro, RJ, Brazil. thays.vieira.tcsv@gmail.com.|Department of Biomedicine, Federal Fluminense University- UFF, Niterói, RJ, Brazil. thays.vieira.tcsv@gmail.com.
authors.forename: Thays C S
authors.initials: TC
authors.lastname: Vieira
authors.affiliation: Department of Medicine, Health Sciences Center of Lagarto, Federal University of Sergipe (UFS), Lagarto, SE, Brazil. ccmontesk@yahoo.com.br.
authors.forename: Claudia C K
authors.initials: CC
authors.lastname: Alvim-Pereira
authors.affiliation: Department of Dentistry, Health Sciences Center of Lagarto, Federal University of Sergipe (UFS), Lagarto, SE, Brazil. falvim@ufs.br.
authors.forename: Fabiano
authors.initials: F
authors.lastname: Alvim-Pereira
authors.affiliation: Department of Oral Biology, University of Pittsburgh, Pittsburgh, PA, USA. kbd1@pitt.edu.
authors.forename: Kathleen
authors.initials: K
authors.lastname: Deeley
authors.affiliation: Dental School, Clinical Research Unit and Biology Institute, Federal Fluminense University - UFF, Niterói, RJ, Brazil. jmgranjeiro@gmail.com.|National Institute of Metrology (INMETRO), Niterói, RJ, Brazil. jmgranjeiro@gmail.com.
authors.forename: José M
authors.initials: JM
authors.lastname: Granjeiro
authors.affiliation: Center for Health and Biological Sciences, Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba, Brazil. paula.trevilatto@pucpr.br.
authors.forename: Paula C
authors.initials: PC
authors.lastname: Trevilatto
authors.affiliation: Department of Oral Biology, University of Pittsburgh, Pittsburgh, PA, USA. arv11@pitt.edu.|Department of Oral Biology, School of Dental Medicine, University of Pittsburgh, 3501 Terrace Street, Pittsburgh, PA, 15261, USA. arv11@pitt.edu.
authors.forename: Alexandre R
authors.initials: AR
authors.lastname: Vieira
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D055113:Chronic Periodontitis / Q000235:genetics*; D003430:Cross-Sectional Studies; D004268:DNA-Binding Proteins / Q000235:genetics*; D015921:Dental Implants; D003774:Dental Plaque Index; D017267:Dental Prosthesis Design; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics; D005787:Gene Frequency / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016348:Osseointegration / Q000502:physiology; D057873:Peri-Implantitis / Q000235:genetics*; D010512:Periodontal Index; D020641:Polymorphism, Single Nucleotide / Q000235:genetics
chemical_list: C528897:BRINP3 protein, human; D004268:DNA-Binding Proteins; D015921:Dental Implants; D005819:Genetic Markers