title: Gateway-compatible tissue-specific vectors for plant transformation.
abstract: BACKGROUND
  Understanding regulation of developmental events has increasingly required the use of tissue-specific expression of diverse genes affecting plant growth and environmental responses.
  
  
  RESULTS
  To allow for cloning of presumptive promoters with tissue-specific activities, we created two plant expression vectors with multiple cloning sites upstream of a Gateway cassette for expression of either untagged or YFP-tagged genes of interest. For fast and easy tissue-specific expression of desired genes, we further developed an initial set of Gateway-compatible tissue-specific gene expression vectors that allow for the expression of YFP-tagged or untagged proteins driven by the ALCOHOL DEHYDROGENASE1, CHLOROPHYLL A/B BINDING PROTEIN 1, COBRA LIKE1, EXPANSIN7, LATERAL ORGAN BOUNDARIES-DOMAIN 16, SCARECROW, UBIQUITIN10, and WOODEN LEG upstream regulatory regions.
  
  
  CONCLUSIONS
  These vectors provide an invaluable resource to the plant community, allowing for rapid generation of a variety of tissue-specific expression constructs.
authors.affiliation: Department of Biology, Washington University, St. Louis, MO, 63130, USA. martampaciorek@gmail.com.
authors.forename: Marta
authors.initials: M
authors.lastname: Michniewicz
authors.affiliation: Department of Biology, Washington University, St. Louis, MO, 63130, USA. fricke@wustl.edu.
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Frick
authors.affiliation: Department of Biology, Washington University, St. Louis, MO, 63130, USA. strader@wustl.edu.
authors.forename: Lucia C
authors.initials: LC
authors.lastname: Strader
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics; D000426:Alcohol Dehydrogenase / Q000235:genetics; D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D061146:Chlorophyll Binding Proteins / Q000235:genetics; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000737:chemistry* / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000235:genetics; D014170:Transformation, Genetic; D025801:Ubiquitin / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D001426:Bacterial Proteins; D061146:Chlorophyll Binding Proteins; C560927:EXPANSIN A7 protein, Arabidopsis; C571893:LBD16 protein, Arabidopsis; D008164:Luminescent Proteins; C100978:SCR protein, Arabidopsis; D014157:Transcription Factors; D025801:Ubiquitin; C054562:yellow fluorescent protein, Bacteria; D000426:Alcohol Dehydrogenase