title: MiR-200 modulates coelomocytes antibacterial activities and LPS priming via targeting Tollip in Apostichopus japonicus.
abstract: In order to explore the potential roles of microRNAs (miRNAs) in regulating Toll-like receptor (TLR) pathways, we identified Toll interacting protein as a putative target of miR-200 in Apostichopus japonicus coelomocytes by RNA-seq screening (denoted as AjTollip). The positive expression profiles of miR-200 and AjTollip were detected in both LPS exposure primary coelomocytes and Vibrio splendidus challenge sea cucumber. Co-infection miR-200 mimics significantly elevated the expression of AjTollip and its down-stream molecules. In contrast, miR-200 inhibitor significantly repressed the expression of these TLR-pathway members. More importantly, miR-200 displayed not only to enhance coelomocytes antibacterial activities, but to suppress LPS priming in vitro. Overall, all these results will enhance our understanding on miR-200 regulatory roles in anti-bacterial process in sea cucumber.
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo, Zhejiang Province 315211, PR China.
authors.forename: Zhimeng
authors.initials: Z
authors.lastname: Lv
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo, Zhejiang Province 315211, PR China. Electronic address: lichenghua@nbu.edu.cn.
authors.forename: Chenghua
authors.initials: C
authors.lastname: Li
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo, Zhejiang Province 315211, PR China.
authors.forename: Pengjuan
authors.initials: P
authors.lastname: Zhang
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo, Zhejiang Province 315211, PR China.
authors.forename: Zhenhui
authors.initials: Z
authors.lastname: Wang
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo, Zhejiang Province 315211, PR China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo, Zhejiang Province 315211, PR China.
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Jin
mesh_terms: D000818:Animals; D047908:Intracellular Signaling Peptides and Proteins / Q000276:immunology*; D008070:Lipopolysaccharides; D035683:MicroRNAs / Q000276:immunology*; D047528:Stichopus / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D014733:Vibrio; D014735:Vibrio Infections / Q000276:immunology*
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D008070:Lipopolysaccharides; D035683:MicroRNAs
keywords: Antibacterial activity; LPS priming; Toll interacting protein; miR-200