Suspended, Shrinkage-Free, Electrospun PLGA Nanofibrous Scaffold for Skin Tissue Engineering.
Electrospinning is a technique for creating continuous nanofibrous networks that can architecturally be similar to the structure of extracellular matrix (ECM). However, the shrinkage of electrospun mats is unfavorable for the triggering of cell adhesion and further growth. In this work, electrospun PLGA nanofiber assemblies are utilized to create a scaffold. Aided by a polypropylene auxiliary supporter, the scaffold is able to maintain long-term integrity without dimensional shrinkage. This scaffold is also able to suspend in cell culture medium; hence, keratinocyte cells seeded on the scaffold are exposed to air as required in skin tissue engineering. Experiments also show that human skin keratinocytes can proliferate on the scaffold and infiltrate into the scaffold.
‡College of Automation, Harbin Engineering University, Harbin 150001, China.|∥Faculty of Applied Science and Engineering, University of Toronto, Toronto, Ontario M5S 3G8, Canada.
Changhai
C
Ru
Feilong
F
Wang
‡College of Automation, Harbin Engineering University, Harbin 150001, China.
Ming
M
Pang
Lining
L
Sun
§Jiangsu Institute of Clinical Immunology, First Affiliated Hospital of Soochow University, Soochow University, Suzhou 215006, China.
Ruihua
R
Chen
‡College of Automation, Harbin Engineering University, Harbin 150001, China.|∥Faculty of Applied Science and Engineering, University of Toronto, Toronto, Ontario M5S 3G8, Canada.
Yu
Y
Sun
D001458:Bandages; D049109:Cell Proliferation / Q000502:physiology; D002478:Cells, Cultured; D004595:Electroplating / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000502:physiology; D019344:Lactic Acid / Q000737:chemistry*; D008297:Male; D008422:Materials Testing; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D012399:Rotation; D017768:Skin, Artificial; D023822:Tissue Engineering / Q000295:instrumentation*; D054457:Tissue Scaffolds
D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid
electrospining; keratinocyte; scaffold shrinkage; skin tissue engineering; suspending scaffold