Proteomic database mining opens up avenues utilizing extracellular protein phosphorylation for novel therapeutic applications.
Recent advances in extracellular signaling suggest that extracellular protein phosphorylation is a regulatory mechanism outside the cell. The list of reported active extracellular protein kinases and phosphatases is growing, and phosphorylation of an increasing number of extracellular matrix molecules and extracellular domains of trans-membrane proteins is being documented. Here, we use public proteomic databases, collagens - the major components of the extracellular matrix, extracellular signaling molecules and proteolytic enzymes as examples to assess what the roles of extracellular protein phosphorylation may be in health and disease. We propose that novel tools be developed to help assess the role of extracellular protein phosphorylation and translate the findings for biomedical applications. Furthermore, we suggest that the phosphorylation state of extracellular matrix components as well as the presence of extracellular kinases be taken into account when designing translational medical applications.
Department of Developmental Biology, Harvard Medical School/Harvard School of Dental Medicine, 188 Longwood Avenue, Boston, MA, 02115, USA. garif_yalak@hms.harvard.edu.
Garif
G
Yalak
Department of Developmental Biology, Harvard Medical School/Harvard School of Dental Medicine, 188 Longwood Avenue, Boston, MA, 02115, USA. bjorn_olsen@hms.harvard.edu.
Bjorn R
BR
Olsen
D000595:Amino Acid Sequence; D057225:Data Mining; D030562:Databases, Protein; D005110:Extracellular Space / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011506:Proteins / Q000737:chemistry / Q000627:therapeutic use*; D040901:Proteomics
D011506:Proteins