High-Throughput Dose-Response Measurement Using a Label-Free Microarray-in-Microplate Assay Platform.
Microarray-based binding assays facilitate the discovery of protein ligands from large collections of small molecules. Hundreds of ligands can be identified, yet only a small portion of them have interfering effects (competitive or noncompetitive) on a specific protein-receptor binding reaction. Further efficient screening of ligands for those with specific modifying effect is needed in order to take the full advantage of throughputs of microarray-based assays for drug discovery. We report a label-free "microarray-in-microplate" assay platform for simultaneous acquisition of at least 32 dose-response curves in a single experiment, each curve having 12 concentration points. When combined with ligand discovery, this makes the microarray-based platform a true high-throughout means of finding inhibitors to specific protein-receptor reactions starting from a large collection of small-molecule libraries.
Department of Physics, University of California at Davis, Davis, California 95616, United States.
J P
JP
Landry
Department of Physics, University of California at Davis, Davis, California 95616, United States.
G
G
Malovichko
Department of Physics, University of California at Davis, Davis, California 95616, United States.
X D
XD
Zhu
D001681:Biological Assay / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D055767:Immobilized Proteins; D008024:Ligands; D046228:Microarray Analysis / Q000295:instrumentation*; D013194:Staining and Labeling
D055767:Immobilized Proteins; D008024:Ligands