An aromatic cage is required but not sufficient for binding of Tudor domains of the Polycomblike protein family to H3K36me3.
Polycomblike (Pcl) proteins are important transcriptional regulators and components of the Polycomb Repressive Complex 2 (PRC2). The Tudor domains of human homologs PHF1 and PHF19 have been found to recognize trimethylated lysine 36 of histone H3 (H3K36me3); however, the biological role of Tudor domains of other Pcl proteins remains poorly understood. Here, we characterize the molecular basis underlying histone binding activities of the Tudor domains of the Pcl family. In contrast to a predominant view, we found that the methyl lysine-binding aromatic cage is necessary but not sufficient for recognition of H3K36me3 by these Tudor domains and that a hydrophobic patch, adjacent to the aromatic cage, is also required.
a Department of Pharmacology; University of Colorado School of Medicine ; Aurora , CO , USA.
Jovylyn
J
Gatchalian
Molly C
MC
Kingsley
Stacey D
SD
Moslet
Ruben D
RD
Rosas Ospina
Tatiana G
TG
Kutateladze
D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics; D044127:Epigenesis, Genetic; D011495:Histone-Lysine N-Methyltransferase / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008745:Methylation; D019906:Nuclear Magnetic Resonance, Biomolecular; D063151:Polycomb Repressive Complex 2 / Q000737:chemistry* / Q000235:genetics; D063146:Polycomb-Group Proteins / Q000737:chemistry* / Q000235:genetics; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary
D004268:DNA-Binding Proteins; C570567:MTF2 protein, human; C112309:PHF1 protein, human; D063146:Polycomb-Group Proteins; D011495:Histone-Lysine N-Methyltransferase; D063151:Polycomb Repressive Complex 2
Tudor; aromatic cage; histone binding; polycomblike (Pcl); trimethylated lysine 36 of histone H3 (H3K36me3)