[Rapid determination of 28 pesticides and tetramine in whole blood by online gel permeation chromatography coupled with gas chromatography-mass spectrometry].
OBJECTIVE
To study the influences of carbon disulfide (CS2) exposureon fatty acid metabolism in apolipoprotein E (ApoE) knockout mice and C57BL/6J mice.


METHODS
Twenty-four male ApoE knockout mice were randomly and equally divided into four groups: a CS2-exposed normal diet group, a CS2-unexposed normal diet group, a CS2-exposed high-fat diet group, and a CS2-unexposed high-fat diet group. Twenty-four C57BL/6J male mice were divided into four groups in the same way. The CS2-exposed groups were exposed to CS2 (1 g/m³) by static inhalation for 5 hours a day, 5 days a week. After two weeks, the whole blood of mice was collected. Methyl ester derivatization of fatty acids was performed using an acid-catalyzed method. Fatty acid contents before and after exposure were compared by gas chromatography-mass spectroscopy.


RESULTS
There were significant differences in fatty acid contents of mice between the four groups. For the C57BL/6J mice, the arachidic acid contents in the CS2-exposed high-fat diet group were significantly lower than those in the CS2-unexposed high-fat diet group (P = 0.0450). For the ApoE knockout mice, the arachidonic acid contents in the CS2-exposed normal diet group were significantly lower than those in the CS2-unexposed control diet group (P = 0.0452). For the ApoE knockout mice, the γ-linolenic acid contents in the CS2-exposed high-fat diet group were significantly higher than those in the unexposed high-fat diet group (P = 0.0447).


CONCLUSIONS
Exposure to CS2 can induce fatty acid metabolism disorder in mice, indicating that CS2 may increase the risk of atherosclerosis and other cardiovascular diseases.
Department of Occupational Disease and Toxication, Chaoyang Hospital Affiliated to the Capital University of Medical Science.
Bo
B
Zhou
Department of Occupational Disease and Toxication, Chaoyang Hospital Affiliated to the Capital University of Medical Science. E-mail: lhl_bj@sohu.com.
Huiling
H
Li
Department of Occupational Disease and Toxication, Chaoyang Hospital Affiliated to the Capital University of Medical Science.
Fengtong
F
Hao
Department of Occupational Disease and Toxication, Chaoyang Hospital Affiliated to the Capital University of Medical Science.
Jing
J
Ma
Department of Occupational Disease and Toxication, Chaoyang Hospital Affiliated to the Capital University of Medical Science.
Fang
F
Dong
D000818:Animals; D001057:Apolipoproteins E / Q000187:drug effects*; D050197:Atherosclerosis; D002246:Carbon Disulfide / Q000633:toxicity*; D059305:Diet, High-Fat; D008401:Gas Chromatography-Mass Spectrometry; D050356:Lipid Metabolism / Q000187:drug effects*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D012307:Risk Factors
D001057:Apolipoproteins E; D002246:Carbon Disulfide