Retention and transport of an anaerobic trichloroethene dechlorinating microbial culture in anaerobic porous media.
The influence of solution chemistry on microbial transport was examined using the strictly anaerobic trichloroethene (TCE) bioaugmentation culture KB-1(®). A column was employed to determine transport behaviors and deposition kinetics of three distinct functional species in KB-1(®), Dehalococcoides, Geobacter, and Methanomethylovorans, over a range of ionic strengths under a well-controlled anaerobic condition. A quantitative polymerase chain reaction (qPCR) was utilized to enumerate cell concentration and complementary techniques were implemented to evaluate cell surface electrokinetic potentials. Solution chemistry was found to positively affect the deposition rates, which was consistent with calculated Derjaguin-Landau-Verwey-Overbeek (DLVO) interaction energies. Retained microbial profiles showed spatially constant colloid deposition rate coefficients, in agreement with classical colloid filtration theory (CFT). It was interesting to note that the three KB-1(®) species displayed similar transport and retention behaviors under the defined experimental conditions despite their different cell electrokinetic properties. A deeper analysis of cell characteristics showed that factors, such as cell size and shape, concentration, and motility were involved in determining adhesion behavior.
Department of Civil and Environmental Engineering, University of Alberta, 9105 116 Street, Edmonton, Alberta T6G 2W2 Canada.
Huixin
H
Zhang
Department of Civil and Environmental Engineering, University of Alberta, 9105 116 Street, Edmonton, Alberta T6G 2W2 Canada. Electronic address: aulrich@ualberta.ca.
Ania C
AC
Ulrich
Department of Civil and Environmental Engineering, University of Alberta, 9105 116 Street, Edmonton, Alberta T6G 2W2 Canada. Electronic address: yang.liu@ualberta.ca.
Yang
Y
Liu
D000693:Anaerobiosis; D001673:Biodegradation, Environmental; D001692:Biological Transport; D002713:Chlorine / Q000378:metabolism; D041862:Chloroflexi / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D019641:DNA, Archaeal / Q000235:genetics / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D045302:Geobacter / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D007700:Kinetics; D017019:Methanosarcinaceae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D008828:Microbiological Techniques / Q000379:methods; D016062:Porosity; D013045:Species Specificity; D014241:Trichloroethylene / Q000378:metabolism*
D003470:Culture Media; D019641:DNA, Archaeal; D004269:DNA, Bacterial; D014241:Trichloroethylene; D002713:Chlorine
Anaerobic; DLVO theory; Porous media; TCE degradation microbial culture; Transport and deposition