Application and reactivation of magnetic nanoparticles in Microcystis aeruginosa harvesting.
This study developed a magnetic nanoparticles (MNPs) harvesting and reactivation technique for rapid cyanobacteria Microcystis aeruginosa separation. The harvesting of raw MNPs achieved high efficiency of 99.6% with the MNPs dosage of 0.58g MNPs/g dry-biomass, but gradually decreased to 59.1% when directly reused 5 times. With extra ultrasonic chloroform:methanol solvent treatment, the MNPs can be effectively reactivated for M. aeruginosa harvesting with 60% efficiency after 5 times reactivation and the separation efficiency kept above 93% with 0.20g MNPs/g dry-biomass dosage. The cyanobacteria-MNPs complex can be effectively disrupted by ultrasonic chloroform:methanol solvent treatment and the zeta potential was recovered for MNPs electrostatic attraction. The MNPs adsorption followed the Langmuir isotherm, and the maximum adsorption capacity and Langmuir constant was 3.74g dry-biomass/g and 311.64L/g respectively. This MNPs reactivation technique can achieve low energy separation and reduce MNPs consumption by 67%, providing potential engineering implementation for cyanobacterial biomass harvesting.
College of Agriculture, Guangdong Ocean University, Zhanjiang 524088, PR China; Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, PR China; Lancaster Environment Centre, Lancaster University, Lancaster LA1 2YQ, UK.
Zhong
Z
Lin
School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, PR China.
Yunfeng
Y
Xu
College of Agriculture, Guangdong Ocean University, Zhanjiang 524088, PR China.
Zhen
Z
Zhen
School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, PR China.
Yu
Y
Fu
College of Water Sciences, Beijing Normal University, Beijing 100875, PR China.
Yueqiao
Y
Liu
College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, PR China.
Wenyan
W
Li
Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, PR China.
Chunling
C
Luo
College of Water Sciences, Beijing Normal University, Beijing 100875, PR China.
Aizhong
A
Ding
Lancaster Environment Centre, Lancaster University, Lancaster LA1 2YQ, UK. Electronic address: d.zhang@lancaster.ac.uk.
Dayi
D
Zhang
D061249:Batch Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D005590:Fractional Precipitation / Q000379:methods*; D060526:Magnetic Fields; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000528:radiation effects / Q000648:ultrastructure; D046931:Microcystis / Q000737:chemistry* / Q000302:isolation & purification* / Q000528:radiation effects; D013010:Sonication / Q000379:methods
D058185:Magnetite Nanoparticles
Cyanobacteria harvesting; Electrostatic attraction; Magnetic nanoparticles (MNPs); Microcystis aeruginosa