Image distortion and its correction in linear galvanometric mirrors-based laser-scanning microscopy.
To simplify imaging focusing and calibration tasks, a laser-scanning microscope needs to scan ata moderate frame rate. The inertia of a galvanometric scanner leads to time delays when following external commands, which subsequently introduces image distortions that deteriorate as scan frequency increases. Sinusoidal and triangular waveforms were examined as fast axis driving patterns. The interplay among driving pattern, frequency, sampling rate, phase shift, linear scanning range, and their effect on reconstructed images was discussed. Utilizing position feedback from the linear galvo scanners, the effect of response time could be automatically compensated in real time. Precompensated triangular driving waveform offered the least amount of image distortion.
University of British Columbia, Photomedicine Institute, Department of Dermatology and Skin Science, 835 West 10th Avenue, Vancouver, British Columbia V5Z 4E8, CanadabBritish Columbia Cancer Agency Research Centre (BCCRC) Imaging Unit, Integrative Oncolog.
Wenbo
W
Wang
University of British Columbia, Photomedicine Institute, Department of Dermatology and Skin Science, 835 West 10th Avenue, Vancouver, British Columbia V5Z 4E8, CanadabBritish Columbia Cancer Agency Research Centre (BCCRC) Imaging Unit, Integrative Oncolog.
Zhenguo
Z
Wu
University of British Columbia, Photomedicine Institute, Department of Dermatology and Skin Science, 835 West 10th Avenue, Vancouver, British Columbia V5Z 4E8, CanadabBritish Columbia Cancer Agency Research Centre (BCCRC) Imaging Unit, Integrative Oncolog.
Haishan
H
Zeng
D016477:Artifacts; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005246:Feedback; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods*; D007909:Lenses; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation*; D018613:Microscopy, Confocal / Q000295:instrumentation*; D019047:Phantoms, Imaging; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity