Influence of different surface modification treatments on silk biotextiles for tissue engineering applications.
Biotextile structures from silk fibroin have demonstrated to be particularly interesting for tissue engineering (TE) applications due to their high mechanical strength, interconnectivity, porosity, and ability to degrade under physiological conditions. In this work, we described several surface treatments of knitted silk fibroin (SF) scaffolds, namely sodium hydroxide (NaOH) solution, ultraviolet radiation exposure in an ozone atmosphere (UV/O3) and oxygen (O2) plasma treatment followed by acrylic acid (AAc), vinyl phosphonic acid (VPA), and vinyl sulfonic acid (VSA) immersion. The effect of these treatments on the mechanical properties of the textile constructs was evaluated by tensile tests in dry and hydrated states. Surface properties such as morphology, topography, wettability and elemental composition were also affected by the applied treatments. The in vitro biological behavior of L929 fibroblasts revealed that cells were able to adhere and spread both on the untreated and surface-modified textile constructs. The applied treatments had different effects on the scaffolds' surface properties, confirming that these modifications can be considered as useful techniques to modulate the surface of biomaterials according to the targeted application.
3B's Research Group-Biomaterials, Biodegradables and Biomimetics, Universidade do Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909, Caldas das Taipas, Portugal.|ICVS/3B's-PT Government Associated Laboratory, Braga, Guimarães, Portugal.
Viviana P
VP
Ribeiro
3B's Research Group-Biomaterials, Biodegradables and Biomimetics, Universidade do Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909, Caldas das Taipas, Portugal.|ICVS/3B's-PT Government Associated Laboratory, Braga, Guimarães, Portugal.
Lília R
LR
Almeida
3B's Research Group-Biomaterials, Biodegradables and Biomimetics, Universidade do Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909, Caldas das Taipas, Portugal.|ICVS/3B's-PT Government Associated Laboratory, Braga, Guimarães, Portugal.
Ana R
AR
Martins
3B's Research Group-Biomaterials, Biodegradables and Biomimetics, Universidade do Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909, Caldas das Taipas, Portugal.|ICVS/3B's-PT Government Associated Laboratory, Braga, Guimarães, Portugal.
Iva
I
Pashkuleva
3B's Research Group-Biomaterials, Biodegradables and Biomimetics, Universidade do Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909, Caldas das Taipas, Portugal.|ICVS/3B's-PT Government Associated Laboratory, Braga, Guimarães, Portugal.
Alexandra P
AP
Marques
CeNTI, Centre for Nanotechnology and Smart Materials, V.N. Famalicão, Portugal.
Ana S
AS
Ribeiro
CeNTI, Centre for Nanotechnology and Smart Materials, V.N. Famalicão, Portugal.
Carla J
CJ
Silva
CITEVE, Technological Centre for Textile and Clothing Industry, V.N. Famalicão, Portugal.
Graça
G
Bonifácio
3B's Research Group-Biomaterials, Biodegradables and Biomimetics, Universidade do Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909, Caldas das Taipas, Portugal.|ICVS/3B's-PT Government Associated Laboratory, Braga, Guimarães, Portugal.
Rui A
RA
Sousa
3B's Research Group-Biomaterials, Biodegradables and Biomimetics, Universidade do Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909, Caldas das Taipas, Portugal.|ICVS/3B's-PT Government Associated Laboratory, Braga, Guimarães, Portugal.
Rui L
RL
Reis
3B's Research Group-Biomaterials, Biodegradables and Biomimetics, Universidade do Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909, Caldas das Taipas, Portugal.|ICVS/3B's-PT Government Associated Laboratory, Braga, Guimarães, Portugal.|CBQF-Center for Biotechnology and Fine Chemistry, School of Biotechnology, Portuguese Catholic University, Porto, 4200-401, Portugal.
Ana L
AL
Oliveira
D000179:Acrylates / Q000737:chemistry; D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D008422:Materials Testing; D051379:Mice; D058626:Plasma Gases / Q000737:chemistry; D047011:Silk / Q000737:chemistry*; D013784:Textiles; D023822:Tissue Engineering; D017155:Wettability
D000179:Acrylates; D020099:Coated Materials, Biocompatible; D058626:Plasma Gases; D047011:Silk; C036658:acrylic acid
biotextile; scaffold; silk fibroin; surface modification; tissue engineering