Optimization of synergism of a recombinant auxiliary activity 9 from Chaetomium globosum with cellulase in cellulose hydrolysis.
Auxiliary activity family 9 (AA9, formerly known as glycoside hydrolase family 61 or polysaccharide monooxygenase) is a group of fungal proteins that were recently found to have a significant synergism with cellulase in cellulose hydrolysis via the oxidative cleavage of glycosidic bonds of cellulose chains. In this study, we report the active expression of a recombinant fungal AA9 from Chaetomium globosum (CgAA9) in a bacterial host, Escherichia coli, and the optimization of its synergistic activity in cellulose hydrolysis by using cellulase. The recombinant CgAA9 (0.9 mg/g cellulose) exhibited 1.7-fold synergism in the hydrolysis of Avicel when incubated with 0.9 filter paper units of Celluclast 1.5 L/g cellulose. The first study of the active expression of AA9 using a bacterial host and its synergistic optimization could be useful for the industrial application of AA9 for the saccharification of lignocellulose.
Department of Biotechnology, Korea University Graduate School, Seoul, 136-713, Republic of Korea.
In Jung
IJ
Kim
Pohang Accelerator Laboratory, Pohang University of Science and Technology, Pohang, 790-784, Republic of Korea.
Ki Hyun
KH
Nam
Department of Biotechnology, Korea University Graduate School, Seoul, 136-713, Republic of Korea.
Eun Ju
EJ
Yun
Department of Biotechnology, Korea University Graduate School, Seoul, 136-713, Republic of Korea.
Sooah
S
Kim
Department of Biotechnology, Korea University Graduate School, Seoul, 136-713, Republic of Korea.
Hak Jin
HJ
Youn
Department of Biotechnology, Korea University Graduate School, Seoul, 136-713, Republic of Korea.
Hee Jin
HJ
Lee
Department of Biotechnology, Korea University Graduate School, Seoul, 136-713, Republic of Korea.
In-Geol
IG
Choi
Department of Biotechnology, Korea University Graduate School, Seoul, 136-713, Republic of Korea. khekim@korea.ac.kr.
Kyoung Heon
KH
Kim
D002482:Cellulose / Q000378:metabolism*; D002597:Chaetomium / Q000201:enzymology* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000235:genetics* / Q000378:metabolism*; D006868:Hydrolysis; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D011994:Recombinant Proteins; D002482:Cellulose; D006026:Glycoside Hydrolases
AA9; Cellulase; Cellulose hydrolysis; Chaetomium globosum; GH61; Synergism