Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+ CD45RB high T Cells into Immunodeficient Mice.
There are many different animal models available for studying the pathogenesis of human inflammatory bowel diseases (IBD), each with its own advantages and disadvantages. We describe here an experimental colitis model that is initiated by adoptive transfer of syngeneic splenic CD4(+)CD45RB(high) T cells into T and B cell deficient recipient mice. The CD4(+)CD45RB(high) T cell population that largely consists of naïve effector cells is capable of inducing chronic intestinal inflammation, closely resembling key aspects of human IBD. This method can be manipulated to study aspects of disease onset and progression. Additionally it can be used to study the function of innate, adaptive, and regulatory immune cell populations, and the role of environmental exposures, i.e., the microbiota, in intestinal inflammation. In this article we illustrate the methodology for inducing colitis with a step-by-step protocol. This includes a video demonstration of key technical aspects required to successfully develop this murine model of experimental colitis for research purposes.
Center for Gastrointestinal Biology and Disease, University of North Carolina at Chapel Hill; Department of Microbiology and Immunology, University of North Carolina at Chapel Hill; erin_klein@med.unc.edu.
Erin C
EC
Steinbach
Center for Gastrointestinal Biology and Disease, University of North Carolina at Chapel Hill.
Gregory R
GR
Gipson
Center for Gastrointestinal Biology and Disease, University of North Carolina at Chapel Hill; Department of Genetics, University of North Carolina at Chapel Hill; Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill.
Shehzad Z
SZ
Sheikh
D019264:Adoptive Transfer / Q000379:methods*; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000473:pathology / Q000637:transplantation*; D003092:Colitis / Q000276:immunology* / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007249:Inflammation / Q000473:pathology; D017493:Leukocyte Common Antigens / Q000276:immunology; D008297:Male; D051379:Mice
D017493:Leukocyte Common Antigens