Protein attachment to silane-functionalized porous silicon: A comparison of electrostatic and covalent attachment.
Porous silicon (pSi) is a prosperous biomaterial, biocompatible, and biodegradable. Obtaining regularly functionalized pSi surfaces is required in many biotechnology applications. Silane-PEG-NHS (triethoxysilane-polyethylene-glycol-N-hydroxysuccinimide) is useful for single-molecule studies due to its ability to attach to only one biomolecule. We investigate the functionalization of pSi with silane-PEG-NHS and compare it with two common grafting agents: APTMS (3-aminopropylotrimethoxysilane) as electrostatic linker, and APTMS modified with glutaraldehyde as covalent spacer. We show the arrangement of two proteins (collagen and bovine serum albumin) as a function of the functionalization and of the pore size. FTIR is used to demonstrate correct functionalization while fluorescence confocal microscopy reveals that silane-PEG-NHS results in a more uniform protein distribution. Reflection interference spectroscopy (RIfS) is used to estimate the attachment of linker and proteins. The results open a way to obtain homogenous chemical modified silicon supports with a great value in biosensing, drug delivery and cell biology.
Departament d'Enginyeria Electrònica, Elèctrica i Automàtica, Universitat Rovira i Virgili, Avda. Països Catalans 26, Tarragona 43007, Spain.
Malgorzata
M
Baranowska
Departament d'Enginyeria Electrònica, Elèctrica i Automàtica, Universitat Rovira i Virgili, Avda. Països Catalans 26, Tarragona 43007, Spain.
Agata J
AJ
Slota
Departament d'Enginyeria Electrònica, Elèctrica i Automàtica, Universitat Rovira i Virgili, Avda. Països Catalans 26, Tarragona 43007, Spain.
Pinkie J
PJ
Eravuchira
Departament d'Enginyeria Electrònica, Elèctrica i Automàtica, Universitat Rovira i Virgili, Avda. Països Catalans 26, Tarragona 43007, Spain.
Maria
M
Alba
Departament d'Enginyeria Electrònica, Elèctrica i Automàtica, Universitat Rovira i Virgili, Avda. Països Catalans 26, Tarragona 43007, Spain.
Pilar
P
Formentin
Departament d'Enginyeria Electrònica, Elèctrica i Automàtica, Universitat Rovira i Virgili, Avda. Països Catalans 26, Tarragona 43007, Spain.
Josep
J
Pallarès
Departament d'Enginyeria Electrònica, Elèctrica i Automàtica, Universitat Rovira i Virgili, Avda. Països Catalans 26, Tarragona 43007, Spain. Electronic address: josep.ferre@urv.cat.
Josep
J
Ferré-Borrull
Departament d'Enginyeria Electrònica, Elèctrica i Automàtica, Universitat Rovira i Virgili, Avda. Països Catalans 26, Tarragona 43007, Spain. Electronic address: lluis.marsal@urv.cat.
Lluís F
LF
Marsal
D000818:Animals; D001665:Binding Sites; D001672:Biocompatible Materials; D002417:Cattle; D003094:Collagen / Q000737:chemistry*; D005976:Glutaral / Q000737:chemistry; D017953:Isocyanates / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry*; D016062:Porosity; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012821:Silanes / Q000737:chemistry*; D012825:Silicon / Q000737:chemistry*; D055672:Static Electricity; D013388:Succinimides / Q000737:chemistry*
C514436:3-isocyanatopropyl trimethoxysilane; D001672:Biocompatible Materials; D017953:Isocyanates; D012821:Silanes; D013388:Succinimides; C490327:poly(ethylene glycol)silane; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D003094:Collagen; C001426:N-hydroxysuccinimide; D005976:Glutaral; D012825:Silicon
APTMS; Bovine serum albumin; Collagen; GTA; Porous silicon; Reflection interference spectroscopy (RIfS); Silane–PEG–NHS