Kinamycin biosynthesis employs a conserved pair of oxidases for B-ring contraction.
The biosynthesis of diazobenzofluorene kinamycins requires a hitherto uncharacterized B-ring contraction. Via detailed genetic and enzymatic analyses we unambiguously characterized the conserved pairs of oxidases, AlpJ and AlpK homologs, as nature's machinery for benzofluorenone formation, which paves the way for the investigation of the following diazo assembly.
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, 100101 Beijing, China. fankq@im.ac.cn yangkq@im.ac.cn.
Bin
B
Wang
Jinwei
J
Ren
Liyuan
L
Li
Fang
F
Guo
Guohui
G
Pan
Guomin
G
Ai
Bertrand
B
Aigle
Keqiang
K
Fan
Keqian
K
Yang
D002227:Carbazoles / Q000737:chemistry / Q000378:metabolism; D015394:Molecular Structure; D010088:Oxidoreductases / Q000737:chemistry / Q000378:metabolism*; D013237:Stereoisomerism; D000095602:Angucyclines and Angucyclinones
D002227:Carbazoles; D010088:Oxidoreductases; C088442:kinamycin D; D000095602:Angucyclines and Angucyclinones