Book lung development in the embryo, postembryo and first instar of the cobweb spider, Parasteatoda tepidariorum C. L Koch, 1841 (Araneomorphae, Theridiidae).
Light and electron microscopy were used to compare spider book lung development with earlier studies of the development of horseshoe crab book gills and scorpion book lungs. Histological studies at the beginning of the 20th century provided evidence that spider and scorpion book lungs begin with outgrowth of a few primary lamellae (respiratory furrows, saccules) from the posterior surface of opisthosomal limb buds, reminiscent of the formation of book gills in the horseshoe crab. In spider embryos, light micrographs herein also show small primary lamellae formed at the posterior surface of opisthosomal limb buds. Later, more prominent primary lamellae extend into each book lung sinus from the inner wall of the book lung operculum formed from the limb bud. It appears most primary lamellae continue developing and become part of later book lungs, but there is variation in the rate and sequence of development. Electron micrographs show the process of air channel formation from parallel rows of precursor cells: mode I (cord hollowing), release of secretory vesicles into the extracellular space and mode II (cell hollowing), alignment and fusion of intracellular vesicles. Cell death (cavitation) is much less common but occurs in some places. Results herein support the early 20th century hypotheses that 1) book lungs are derived from book gills and 2) book lungs are an early step in the evolution of spider tracheae.
Department of Biology, University of California, Riverside, CA 92521, USA. Electronic address: roger.farley@ucr.edu.
Roger D
RD
Farley
D000818:Animals; D005075:Biological Evolution; D004625:Embryo, Nonmammalian / Q000033:anatomy & histology / Q000196:embryology / Q000648:ultrastructure; D008168:Lung / Q000033:anatomy & histology / Q000196:embryology / Q000254:growth & development / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D013112:Spiders / Q000033:anatomy & histology / Q000196:embryology / Q000254:growth & development* / Q000648:ultrastructure
Book lungs; Epithelial morphogenesis; Horseshoe crab; Scorpion; Secretion; Spider