Epidermis-dermis junction as a novel location for bone marrow-derived cells to reside in response to ionizing radiation.
Bone marrow-derived cells (BMDCs) can migrate into the various organs in the mice irradiated by ionizing radiation (IR). However, it may not be the case in the skin. While IR is used for bone marrow (BM) transplantation, studying with the epidermal sheets demonstrated that the BMDC recruitment is extraordinarily rare in epidermis in the mouse. Herein, using the chimera mice with BM from green fluorescent protein (GFP) transgenic mice, we simply examined if BMDCs migrate into any layers in the total skin, as opposed to the epidermal sheets, in response to IR. Interestingly, we identified the presence of GFP-positive (GFP(+)) cells in the epidermis-dermis junction in the total skin sections although the epidermal cell sheets failed to have any GFP cells. To examine a possibility that the cells in the junction could be mechanically dissociated during separating epidermal sheets, we then salvaged such dissociated cells and examined its characteristics. Surprisingly, some GFP(+) cells were found in the salvaged cells, indicating that these cells could be derived from BM. In addition, such BMDCs were also associated with inflammation in the junction. In conclusion, BMDCs can migrate to and reside in the epidermis-dermis junction after IR.
Division of Anatomy and Cell Biology, Shiga University of Medical Science, Shiga, Japan. Electronic address: jokano@belle.shiga-med.ac.jp.
Junko
J
Okano
Department of Stem Cell Biology and Regenerative Medicine, Shiga University of Medical Science, Shiga, Japan.
Hideto
H
Kojima
Department of Stem Cell Biology and Regenerative Medicine, Shiga University of Medical Science, Shiga, Japan.
Miwako
M
Katagi
Department of Internal Medicine, Shiga University of Medical Science, Shiga, Japan.
Yuki
Y
Nakae
Department of Stem Cell Biology and Regenerative Medicine, Shiga University of Medical Science, Shiga, Japan.
Tomoya
T
Terashima
TMK Project, Medical Innovation Center, Kyoto University Graduate School of Medicine, Kyoto, Japan.
Takahiko
T
Nakagawa
Division of Anatomy and Cell Biology, Shiga University of Medical Science, Shiga, Japan.
Takeshi
T
Kurakane
Division of Anatomy and Cell Biology, Shiga University of Medical Science, Shiga, Japan.
Naoki
N
Okamoto
Division of Anatomy and Cell Biology, Shiga University of Medical Science, Shiga, Japan.
Keita
K
Morohashi
Department of Internal Medicine, Shiga University of Medical Science, Shiga, Japan.
Hiroshi
H
Maegawa
Division of Anatomy and Cell Biology, Shiga University of Medical Science, Shiga, Japan.
Jun
J
Udagawa
D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology* / Q000528:radiation effects*; D002465:Cell Movement / Q000502:physiology* / Q000528:radiation effects*; D002478:Cells, Cultured; D020405:Dermis / Q000502:physiology* / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D004817:Epidermis / Q000502:physiology* / Q000528:radiation effects; D051379:Mice; D008810:Mice, Inbred C57BL; D011829:Radiation Dosage; D012879:Skin Physiological Phenomena / Q000528:radiation effects*
Bone marrow transplantation; Bone marrow-derived cells; Epidermis–dermis junction; Inflammation; Ionizing radiation; Skin