Three-dimensional zinc incorporated borosilicate bioactive glass scaffolds for rodent critical-sized calvarial defects repair and regeneration.
The biomaterials with high osteogenic ability are being intensively investigated. In this study, we evaluated the bioactivity and osteogenesis of BG-Zn scaffolds in vitro and in vivo with a rodent calvarial defects model. Zinc containing borosilicate bioactive glass was prepared by doping glass with 1.5, 5 and 10 wt.% ZnO (denoted as BG-1.5Zn, BG-5Zn and BG-10Zn, respectively). When immersed in simulated body fluid, dopant ZnO retarded the degradation process, but did not affect the formation of hydroxyapatite (HA) after long-period soaking. BG-Zn scaffolds showed controlled release of Zn ions into the medium for over 8 weeks. Human bone marrow derived stem cells (hBMSCs) attached well on the BG-1.5Zn and BG-5Zn scaffolds, which exhibited no cytotoxicity to hBMSCs. In addition, the alkaline phosphatase activity of the hBMSCs increased with increasing dopant amount in the glass, while the BG-10Zn group showed over-dose of Zn. Furthermore, when implanted in rat calvarial defects for 8 weeks, the BG-5Zn scaffolds showed a significantly better capacity to regenerate bone tissue compared to the non-doping scaffolds. Generally, these results showed the BG-Zn scaffolds with high osteogenic capacity will be promising candidates using in bone tissue repair and regeneration.
School of Materials Science and Engineering, Tongji University, Shanghai 2001804, China.
Hui
H
Wang
Department of Orthopedic Surgery, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, China.
Shichang
S
Zhao
Department of Materials Science and Engineering, Missouri University of Science and Technology, Rolla, MO 65409, USA.
Wei
W
Xiao
School of Materials Science and Engineering, Tongji University, Shanghai 2001804, China.
Xu
X
Cui
School of Materials Science and Engineering, Tongji University, Shanghai 2001804, China.
Wenhai
W
Huang
Department of Materials Science and Engineering, Missouri University of Science and Technology, Rolla, MO 65409, USA.
Mohamed N
MN
Rahaman
Department of Orthopedic Surgery, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, China. Electronic address: shzhangchangqing@163.com.
Changqing
C
Zhang
School of Materials Science and Engineering, Tongji University, Shanghai 2001804, China. Electronic address: wdpshk@tongji.edu.cn.
Deping
D
Wang
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001861:Bone Regeneration; D001842:Bone and Bones / Q000473:pathology / Q000503:physiopathology* / Q000648:ultrastructure; D001896:Boron Compounds / Q000737:chemistry; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D005898:Glass / Q000737:chemistry*; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning / Q000379:methods; D017207:Rats, Sprague-Dawley; D017640:Silicates / Q000737:chemistry*; D054457:Tissue Scaffolds / Q000737:chemistry*; D055114:X-Ray Microtomography; D015032:Zinc / Q000737:chemistry*
D001896:Boron Compounds; D017640:Silicates; D000469:Alkaline Phosphatase; D015032:Zinc
Bioactivity; Borosilicate bioactive glass; Osteogenesis; Scaffolds; Zinc-doped