A fast and selective two-photon phosphorescent probe for the imaging of nitric oxide in mitochondria.
Nitric oxide (NO) is a vitally important cellular messenger molecule related to numerous physiological events and diseases. It is more than 10 years now that mitochondria are suspected to be sources of nitric oxide. Hence, mitochondrial NO tracking probes play an indispensable role in NO behavior analysis. However, a majority of previously reported NO probes can only be employed under one-photon microscopy, often with several drawbacks during application. In the present study, an iridium(III) complex containing 1,10-phenanthroline-5,6-diamine (Ir-Mito-NO) was synthesized and determined to possess high specificity to NO in mitochondria, low cytotoxicity, and rapid and specific "off-on" two-photon phosphorescence. Thus, this complex was developed to image mitochondrial NO levels in living cells, 3D multicellular spheroids and zebrafish.
MOE Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, PR China.
Xiang
X
Chen
MOE Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, PR China.
Lingli
L
Sun
MOE Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, PR China.
Yu
Y
Chen
MOE Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, PR China.
Xiaolin
X
Cheng
MOE Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, PR China.
Weijun
W
Wu
MOE Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, PR China.
Liangnian
L
Ji
MOE Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, PR China. Electronic address: ceschh@mail.sysu.edu.cn.
Hui
H
Chao
D000818:Animals; D002448:Cell Adhesion; D003959:Diamines / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D007495:Iridium / Q000737:chemistry; D049449:Luminescence; D013058:Mass Spectrometry; D018613:Microscopy, Confocal / Q000295:instrumentation*; D008928:Mitochondria / Q000378:metabolism*; D015335:Molecular Probes / Q000737:chemistry*; D009569:Nitric Oxide / Q000737:chemistry*; D010618:Phenanthrolines / Q000737:chemistry; D017785:Photons; D018874:Spheroids, Cellular; D014407:Tumor Cells, Cultured; D015027:Zebrafish
C000590274:1,10-phenanthroline-5,6-diamine; D003959:Diamines; D015335:Molecular Probes; D010618:Phenanthrolines; D009569:Nitric Oxide; D007495:Iridium
Iridium complex; Mitochondria; Nitric oxide; Two-photon probe; Zebrafish