Atrial natriuretic peptide and aldosterone synthase gene in essential hypertension: a case-control study.
The renin-angiotensin-aldosterone system (RAAS) and their candidate genes are principally involved in regulation of blood pressure through salt-water homeostasis. Atrial natriuretic peptide (ANP) and Aldosterone synthase (CYP11B2) are the important RAAS mediators, play a major role in hypertension through regulation of cardiorenal homeostasis and water-electrolytes balance, respectively. Present study reports the expression of ANP and CYP11B2 gene at mRNA and proteins levels in patients with essential hypertension in North Indian subjects. Gene expression at mRNA and protein levels was carried out by Real time PCR and Western blot, respectively. We found a significant down regulation in the ANP gene expression at mRNA (85%) and protein (72.6%) levels and significant increase in the CYP11B2 protein expression in patients as compared to controls. A significant increase in Serum creatinine (14.6%), Sodium (1.15%) and decrease in the Blood urea (8.18%) and Potassium (2.32%) levels were also observed among the patients group having higher expression (based on median delta-CT value) in comparison to the lower expression of CYP11B2 gene. Our results suggest that the down-regulation of ANP gene expression at mRNA and protein levels and up-regulated CYP11B2 protein expression levels may be correlated with the essential hypertension and could serve as circulating prognostic biomarkers for essential hypertension.
Dr. B. R. Ambedkar Centre for Biomedical Research, University of Delhi, Delhi 110007, India.
Sudhir
S
Chandra
Dr. B. R. Ambedkar Centre for Biomedical Research, University of Delhi, Delhi 110007, India.
Daman
D
Saluja
Department of Cardiology, All India Institute of Medical Sciences, New Delhi 110029, India.
Rajiv
R
Narang
Department of Pharmacology, All India Institute of Medical Sciences, New Delhi 110029, India.
Jagriti
J
Bhatia
Dr. B. R. Ambedkar Centre for Biomedical Research, University of Delhi, Delhi 110007, India. Electronic address: kamna605@gmail.com.
Kamna
K
Srivastava
D000328:Adult; D000368:Aged; D009320:Atrial Natriuretic Factor / Q000235:genetics*; D001483:Base Sequence; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D016022:Case-Control Studies; D019405:Cytochrome P-450 CYP11B2 / Q000235:genetics*; D017931:DNA Primers; D005260:Female; D006801:Humans; D006973:Hypertension / Q000235:genetics*; D008297:Male; D008875:Middle Aged
D015415:Biomarkers; D017931:DNA Primers; D009320:Atrial Natriuretic Factor; D019405:Cytochrome P-450 CYP11B2
Blood pressure; Gene expression; Renin-angiotensin-aldosterone system