Sleep restores behavioral plasticity to Drosophila mutants.
Given the role that sleep plays in modulating plasticity, we hypothesized that increasing sleep would restore memory to canonical memory mutants without specifically rescuing the causal molecular lesion. Sleep was increased using three independent strategies: activating the dorsal fan-shaped body, increasing the expression of Fatty acid binding protein (dFabp), or by administering the GABA-A agonist 4,5,6,7-tetrahydroisoxazolo-[5,4-c]pyridine-3-ol (THIP). Short-term memory (STM) or long-term memory (LTM) was evaluated in rutabaga (rut) and dunce (dnc) mutants using aversive phototaxic suppression and courtship conditioning. Each of the three independent strategies increased sleep and restored memory to rut and dnc mutants. Importantly, inducing sleep also reverses memory defects in a Drosophila model of Alzheimer's disease. Together, these data demonstrate that sleep plays a more fundamental role in modulating behavioral plasticity than previously appreciated and suggest that increasing sleep may benefit patients with certain neurological disorders.
Department of Anatomy and Neurobiology, Washington University in St. Louis, 660 South Euclid Avenue, St. Louis, MO 63110, USA.
Stephane
S
Dissel
Department of Anatomy and Neurobiology, Washington University in St. Louis, 660 South Euclid Avenue, St. Louis, MO 63110, USA.
Veena
V
Angadi
Queensland Brain Institute, The University of Queensland, Brisbane, QLD 4072, Australia.
Leonie
L
Kirszenblat
Department of Anatomy and Neurobiology, Washington University in St. Louis, 660 South Euclid Avenue, St. Louis, MO 63110, USA.
Yasuko
Y
Suzuki
Centre for Neural Circuits and Behaviour, University of Oxford, Oxford 1 3SR, UK.
Jeff
J
Donlea
Department of Anatomy and Neurobiology, Washington University in St. Louis, 660 South Euclid Avenue, St. Louis, MO 63110, USA.
Markus
M
Klose
Department of Anatomy and Neurobiology, Washington University in St. Louis, 660 South Euclid Avenue, St. Louis, MO 63110, USA.
Zachary
Z
Koch
Department of Anatomy and Neurobiology, Washington University in St. Louis, 660 South Euclid Avenue, St. Louis, MO 63110, USA.
Denis
D
English
Surrey Sleep Research Centre, Faculty of Health and Medical Sciences, University of Surrey, Guildford, Surrey 2 7XH, UK.
Raphaelle
R
Winsky-Sommerer
Queensland Brain Institute, The University of Queensland, Brisbane, QLD 4072, Australia.
Bruno
B
van Swinderen
Department of Anatomy and Neurobiology, Washington University in St. Louis, 660 South Euclid Avenue, St. Louis, MO 63110, USA. Electronic address: shawp@pcg.wustl.edu.
Paul J
PJ
Shaw
D000262:Adenylyl Cyclases / Q000235:genetics*; D000544:Alzheimer Disease / Q000503:physiopathology; D000818:Animals; D030801:Animals, Genetically Modified; D001522:Behavior, Animal / Q000502:physiology*; D004195:Disease Models, Animal; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics / Q000502:physiology*; D050556:Fatty Acid-Binding Proteins / Q000235:genetics; D005260:Female; D007555:Isoxazoles / Q000494:pharmacology; D008297:Male; D057567:Memory, Long-Term / Q000502:physiology; D008570:Memory, Short-Term / Q000187:drug effects / Q000502:physiology; D009154:Mutation; D009943:Organophosphorus Compounds / Q000494:pharmacology; D018079:Receptors, GABA / Q000235:genetics; D012110:Reserpine / Q000494:pharmacology; D012890:Sleep / Q000187:drug effects / Q000502:physiology*
D029721:Drosophila Proteins; D050556:Fatty Acid-Binding Proteins; D007555:Isoxazoles; D009943:Organophosphorus Compounds; D018079:Receptors, GABA; C523245:dnc protein, Drosophila; C068457:3-aminopropyl(methyl)phosphinic acid; D012110:Reserpine; D000262:Adenylyl Cyclases; C479926:Rut protein, Drosophila; C015542:gaboxadol