[Estrogen induced rapid increase of intracellular calcium plays important role in the implantation of mouse blastocysts].
OBJECTIVE
To study the roles of the increased intracellular calcium induced rapidly by estrogen in the implantation of mouse blastocysts.


METHODS
The mouse blastocysts were collected from the female mice on the pregnant day 4, divided into 3 groups: control, E2-BSA and BAPTA +E2-BSA. Immunofluorescence staining, confocal microscopy, embryo and endometrial epithenial cells co-culture and embryo transfer were used to investigate the effect of increased intracellular calcium induced by E2-BSA on the expression and localization of integrins in blastocysts and their adhesion to endometrial epithenial calls (EECs) and implantation into the endometrium.


RESULTS
The increase of intracellular calcium induced rapidly by estrogen could cause the cluster and relocation of integrin av and beta3, and BAPTA might block this effect, the adhesion rate of blastocysts in contol group was 35.5%, BAPTA +E2-BSA group was 26.7% and significantly lower than 65.6% of E2-BSA group (P<0.05), and the implantation rate in BAPTA+E2-BSA group was 11.8%, which was significantly lower than 52.9% of E2-BSA group (P<0.05).


CONCLUSIONS
The rapid increase of intracellular calcium induced by estrogen may cause the relocalization of integrin in blastocysts and their adhesion to ECCs, which is important in the process of implantation.
Shi-mao
SM
Zhang
Lin-lin
LL
Yu
Ting
T
Qu
Dong-zhi
DZ
Yuan
Jin-hu
JH
Zhang
Ya-ping
YP
He
Li-min
LM
Yue
D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D002118:Calcium / Q000378:metabolism*; D018920:Coculture Techniques; D003593:Cytoplasm; D010064:Embryo Implantation; D004624:Embryo Transfer; D004717:Endometrium; D004958:Estradiol; D004967:Estrogens / Q000502:physiology*; D005260:Female; D051379:Mice; D011247:Pregnancy; D012710:Serum Albumin, Bovine
D004967:Estrogens; C043090:estradiol-bovine serum albumin; D012710:Serum Albumin, Bovine; D004958:Estradiol; D002118:Calcium