Kinetics of lymphocyte subpopulations in allogeneic grafted scales of ginbuna crucian carp.
In mammals the rejection of allografts is primarily accomplished by cell-mediated immunity including T cells. Recently, considerable studies reveal the existence of helper and cytotoxic T cell subsets in fish. Here we investigate the kinetics of CD4(+) and CD8α(+) T cells along with sIgM(+) cells and phagocytic cells in an allogeneic scale graft model using ginbuna crucian carp for understanding the mechanisms of cell-mediated immune response. The results showed that CD4(+) T cells first infiltrated into allogeneic scales followed by CD8α(+) and sIgM(+) cells, and finally phagocytic cells appeared in the graft. Furthermore, most of the CD8α(+) T cells appeared on the border of the allografted scales at the time of rejection. These results suggest that T cells play crucial roles and work together with other cell types for completion of allograft rejection.
Department of Veterinary Medicine, Nihon University, Fujisawa, Kanagawa 252-8510, Japan.
Yasuhiro
Y
Shibasaki
Department of Veterinary Medicine, Nihon University, Fujisawa, Kanagawa 252-8510, Japan.
Yuta
Y
Matsuura
Department of Veterinary Medicine, Nihon University, Fujisawa, Kanagawa 252-8510, Japan.
Hideaki
H
Toda
Department of Veterinary Medicine, Nihon University, Fujisawa, Kanagawa 252-8510, Japan.
Nozomi
N
Imabayashi
Department of Veterinary Medicine, Nihon University, Fujisawa, Kanagawa 252-8510, Japan.
Tatsuyuki
T
Nishino
Department of Veterinary Medicine, Nihon University, Fujisawa, Kanagawa 252-8510, Japan.
Kosuke
K
Uzumaki
Department of Veterinary Medicine, Nihon University, Fujisawa, Kanagawa 252-8510, Japan.
Chihiro
C
Hatanaka
Department of Veterinary Medicine, Nihon University, Fujisawa, Kanagawa 252-8510, Japan.
Takeshi
T
Yabu
Department of Veterinary Medicine, Nihon University, Fujisawa, Kanagawa 252-8510, Japan.
Tadaaki
T
Moritomo
Department of Veterinary Medicine, Nihon University, Fujisawa, Kanagawa 252-8510, Japan. Electronic address: nakanishi.teruyuki@nihon-u.ac.jp.
Teruyuki
T
Nakanishi
D064591:Allografts / Q000276:immunology; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002347:Carps / Q000276:immunology*; D002465:Cell Movement; D005393:Fish Diseases / Q000276:immunology*; D006084:Graft Rejection / Q000276:immunology*; D007075:Immunoglobulin M / Q000378:metabolism; D016131:Lymphocyte Subsets / Q000276:immunology*; D010586:Phagocytes / Q000276:immunology*; D016038:Skin Transplantation
D007075:Immunoglobulin M
Allograft rejection; CD4; CD8; Ginbuna crucian carp; Phagocytic cells; Surface IgM positive cell