Significantly improving enzymatic saccharification of high crystallinity index's corn stover by combining ionic liquid [Bmim]Cl-HCl-water media with dilute NaOH pretreatment.
In this study, a pretreatment by combining acidified aqueous ionic liquid 1-butyl-3-methylimidazolium chloride (IL [Bmim]Cl) solution with dilute NaOH extraction was employed to pretreat high crystallinity index (CrI) of corn stover before its enzymatic saccharification. After NaOH extraction, [Bmim]Cl-HCl-water (78.8:1.2:20, w/w/w) media was used for further pretreatment at 130 °C for 30 min. After being enzymatically hydrolyzed for 48 h, corn stover pretreated could be biotransformed into reducing sugars in the yield of 95.1%. Furthermore, SEM, XRD and FTIR analyses of untreated and pretreated corn stovers were examined. It was found that the intact structure was disrupted by combination pretreatment and resulted in a porous and amorphous regenerated cellulosic material that greatly improved enzymatic hydrolysis. Finally, the recovered hydrolyzates obtained from the enzymatic hydrolysis of pretreated corn stovers could be fermented into ethanol efficiently. In conclusion, the combination pretreatment shows high potential application in future.
Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China. Electronic address: heyucai2001@163.com.
Yu-Cai
YC
He
Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
Feng
F
Liu
Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
Lei
L
Gong
Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
Zheng-Zhong
ZZ
Zhu
Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
Yun
Y
Ding
Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
Cheng
C
Wang
Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
Yu-Feng
YF
Xue
Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
Huan
H
Rui
Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
Zhi-Cheng
ZC
Tao
Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
Dan-Ping
DP
Zhang
Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
Cui-Luan
CL
Ma
D050260:Carbohydrate Metabolism / Q000187:drug effects*; D002480:Cellulase / Q000494:pharmacology*; D003460:Crystallization; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation / Q000187:drug effects; D006868:Hydrolysis; D007093:Imidazoles / Q000494:pharmacology*; D012972:Sodium Hydroxide / Q000494:pharmacology*; D014866:Waste Products / Q000032:analysis*; D014867:Water / Q000494:pharmacology*; D003313:Zea mays / Q000737:chemistry*
D007093:Imidazoles; D014866:Waste Products; D014867:Water; D000431:Ethanol; C502841:1-butyl-3-methylimidazolium chloride; D012972:Sodium Hydroxide; D002480:Cellulase
1-Butyl-3-methylimidazolium chloride; Combination pretreatment; Corn stover; Ionic liquid; NaOH