Germline TP53 mutational spectrum in French Canadians with breast cancer.
BACKGROUND
Specific germline mutations in the hereditary breast-ovarian cancer susceptibility (HBC/HBOC) genes, BRCA1, BRCA2 and PALB2, have been shown to recur in French Canadians of Quebec, Canada, and this has been attributed to common ancestors. Germline TP53 mutation carriers are known to segregate in Li-Fraumeni syndrome families, which feature young age of onset breast cancer. We have reported rare TP53 mutation carriers in French Canadian HBC families, though none recurred possibly due to the limited number of cancer families investigated. Here we describe TP53 germline mutations found in French Canadian cancer families provided from hereditary cancer clinics; investigate 37 new BRCA1 and BRCA2 mutation-negative HBC/HBOC families for the TP53 mutations; and assess the frequency of TP53 mutations in a 1235 French Canadian breast cancer cases not selected for family history of cancer.


METHODS
TP53 mutation-positive pedigrees from French Canadian cancer families were provided from local hereditary cancer clinics. Bidirectional Sanger sequencing of all protein encoding exons of TP53 was performed using peripheral blood lymphocyte DNA from breast/ovarian cancer probands from 37 HBC/HBOC families of French Canadian descent. Targeted bidirectional Sanger sequencing assay of regions containing the identified TP53 mutations was performed on 1235 French Canadian breast cancer cases not selected for family history cancer.


RESULTS
Five new TP53 mutations were identified in six pedigrees from hereditary cancer clinics. No deleterious mutations were identified in cancer probands from 37 HBC/HBOC families. A targeted mutation screen of the 1235 breast cancer cases identified a c.844C>T [p.Arg282Trp] mutation carrier. This mutation was also found among the six mutation-positive cancer families provided by the local hereditary cancer clinics. The targeted screen also uncovered a new TP53 mutation, c.685T>C [p.Cys229Arg] that was found in two breast cancer cases. All TP53 mutation carriers were among the 656 women with breast cancer diagnosed less than 50 years of age.


CONCLUSIONS
In all six new TP53 mutations were identified in French Canadians, where two each occurred in independently ascertained cases/families. Although all newly identified breast cancer mutation carriers reported a family history of cancer, none were consistent with features of Li-Fraumeni syndrome families.
Cancer Research Program, The Research Institute of the McGill University Health Centre, 1001 Decarie Boulevard, E02.6217, Montreal, Quebec, H4A 3J1, Canada. suzanna.arcand@mail.mcgill.ca.
Suzanna L
SL
Arcand
Women's College Research Institute, Women's College Hospital, and University of Toronto, Toronto, Ontario, Canada. mohammad.akbari@utoronto.ca.
Mohammed R
MR
Akbari
Département de médecine, Université de Montréal, Montreal, Quebec, Canada. anne-marie.mes-masson@umontreal.ca.|Centre de recherche du Centre hospitalier de l'Université de Montréal et Institut du cancer de Montréal, Montreal, Quebec, Canada. anne-marie.mes-masson@umontreal.ca.
Anne-Marie
AM
Mes-Masson
Centre de recherche du Centre hospitalier de l'Université de Montréal et Institut du cancer de Montréal, Montreal, Quebec, Canada. diane.provencher.chum@ssss.gouv.qc.ca.|Division de gynécologie oncologique Université de Montréal, Montreal, Quebec, Canada. diane.provencher.chum@ssss.gouv.qc.ca.
Diane
D
Provencher
Cancer Research Program, The Research Institute of the McGill University Health Centre, 1001 Decarie Boulevard, E02.6217, Montreal, Quebec, H4A 3J1, Canada. william.foulkes@mcgill.ca.|Lady Davis Institute of the Jewish General Hospital, Montreal, Quebec, Canada. william.foulkes@mcgill.ca.|Program in Cancer Genetics, Departments of Oncology, Human Genetics and Medicine, McGill University, Quebec, Canada. william.foulkes@mcgill.ca.
William D
WD
Foulkes
Women's College Research Institute, Women's College Hospital, and University of Toronto, Toronto, Ontario, Canada. steven.narod@wchospital.ca.
Steven A
SA
Narod
Cancer Research Program, The Research Institute of the McGill University Health Centre, 1001 Decarie Boulevard, E02.6217, Montreal, Quebec, H4A 3J1, Canada. patricia.tonin@mcgill.ca.|Program in Cancer Genetics, Departments of Oncology, Human Genetics and Medicine, McGill University, Quebec, Canada. patricia.tonin@mcgill.ca.
Patricia N
PN
Tonin
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001943:Breast Neoplasms / Q000208:ethnology* / Q000235:genetics*; D002170:Canada; D002648:Child; D002675:Child, Preschool; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics; D018095:Germ-Line Mutation; D006801:Humans; D008297:Male; D008875:Middle Aged; D010375:Pedigree; D011792:Quebec / Q000208:ethnology; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*; D044465:White People / Q000235:genetics*; D055815:Young Adult
D016159:Tumor Suppressor Protein p53