PRGD/PDLLA conduit potentiates rat sciatic nerve regeneration and the underlying molecular mechanism.
Peripheral nerve injury requires optimal conditions in both macro-environment and micro-environment for reestablishment. Though various strategies have been carried out to improve the macro-environment, the underlying molecular mechanism of axon regeneration in the micro-environment provided by nerve conduit remains unclear. In this study, the rat sciatic nerve of 10 mm defect was made and bridged by PRGD/PDLLA nerve conduit. We investigated the process of nerve regeneration using histological, functional and real time PCR analyses after implantation from 7 to 35 days. Our data demonstrated that the ciliary neurotrophic factor highly expressed and up-regulated the downstream signaling pathways, in the case of activated signals, the expressions of axon sprout relative proteins, such as tubulin and growth-associated protein-43, were strongly augmented. Taken together, these data suggest a possible mechanism of axon regeneration promoted by PRGD/PDLLA conduit, which created a micro-environment for enhancement of diffusion of neurotrophic factors secreted by the injured nerve stumps, and activation of molecular signal transduction involved in growth cone, to potentiate the nerve recovery.
State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, PR China; Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan 430070, PR China.
Binbin
B
Li
State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, PR China; Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan 430070, PR China. Electronic address: tongttqq@163.com.
Tong
T
Qiu
School of Chemistry and Biochemistry, The University of Western Australia, Crawley WA 6009, Australia.
K Swaminathan
KS
Iyer
State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, PR China; Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan 430070, PR China.
Qiongjiao
Q
Yan
State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, PR China; Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan 430070, PR China.
Yixia
Y
Yin
State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, PR China; Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan 430070, PR China.
Lijuan
L
Xie
State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, PR China; Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan 430070, PR China.
Xinyu
X
Wang
State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, PR China; Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan 430070, PR China. Electronic address: lishipu46@126.com.
Shipu
S
Li
D000818:Animals; D001369:Axons / Q000473:pathology; D001672:Biocompatible Materials / Q000737:chemistry; D032701:Biomimetics; D020934:Ciliary Neurotrophic Factor / Q000378:metabolism; D003553:Cystine / Q000737:chemistry; D019922:GAP-43 Protein / Q000378:metabolism; D005786:Gene Expression Regulation; D048091:Guided Tissue Regeneration / Q000295:instrumentation* / Q000379:methods; D019344:Lactic Acid / Q000737:chemistry*; D008024:Ligands; D018482:Muscle, Skeletal / Q000294:innervation; D009416:Nerve Regeneration; D009842:Oligopeptides / Q000737:chemistry; D059348:Peripheral Nerve Injuries / Q000628:therapy; D011091:Polyesters / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D012584:Sciatic Nerve / Q000187:drug effects / Q000473:pathology*; D015398:Signal Transduction; D014404:Tubulin / Q000378:metabolism
D001672:Biocompatible Materials; D020934:Ciliary Neurotrophic Factor; D019922:GAP-43 Protein; D008024:Ligands; D009842:Oligopeptides; D011091:Polyesters; D011108:Polymers; D014404:Tubulin; D019344:Lactic Acid; C033616:poly(lactide); D003553:Cystine; C047981:arginyl-glycyl-aspartic acid
Micro-environment; Molecular mechanism; Nerve conduit; Nerve regeneration; Singling pathway