Unexpectedly fast transfer of positron-emittable artificial substrate into N-terminus of peptide/protein mediated by wild-type L/F-tRNA-protein transferase.
This article demonstrates the fastest enzymatic introduction of a positron emission tomography (PET) probe into acceptor peptides/proteins. It is site-specifically introduced at the basic N-terminus of the acceptors by using L/F-transferase in combination with aminoacyl-tRNA synthetase, namely the NEXT-A/PET reaction. Estimated from kinetic analysis, the transfer efficiency of O-(2-fluoromethyl)-L-tyrosine as an artificial amino acid PET probe mediated by the wild-type transferase is almost as good as that of the natural substrate, phenylalanine.
Division of Chemistry and Biochemistry, Department of Medical and Bioengineering Science, Chemical Biology Area, Okayama University, 1-1-1, Tsushimanaka, Okayama, 700-0082, Japan, taki@pc.uec.ac.jp.
Masumi
M
Taki
Hiroyuki
H
Kuroiwa
D000604:Amino Acyl-tRNA Synthetases / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D049268:Positron-Emission Tomography; D014443:Tyrosine / Q000031:analogs & derivatives / Q000737:chemistry
D005456:Fluorescent Dyes; D010455:Peptides; D014443:Tyrosine; D000604:Amino Acyl-tRNA Synthetases