Plant protein kinase substrates identification using protein microarrays.
Protein kinases regulate signaling pathways by phosphorylating their targets. They play critical roles in plant signaling networks. Although many important protein kinases have been identified in plants, their substrates are largely unknown. We have developed and produced plant protein microarrays with more than 15,000 purified plant proteins. Here, we describe a detailed protocol to use these microarrays to identify plant protein kinase substrates via in vitro phosphorylation assays on these arrays.
Department of Plant Biology and the Genome Center, College of Biological Sciences, University of California Davis, LSA1009, One Shields Avenue, Davis, CA, 95616, USA, sma@ucdavis.edu.
Shisong
S
Ma
Savithramma P
SP
Dinesh-Kumar
D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism; D066298:In Vitro Techniques; D010750:Phosphoproteins / Q000302:isolation & purification* / Q000378:metabolism; D010766:Phosphorylation; D040081:Protein Array Analysis / Q000379:methods*; D011494:Protein Kinases / Q000378:metabolism*; D015398:Signal Transduction; D013379:Substrate Specificity
D029681:Arabidopsis Proteins; D010750:Phosphoproteins; D011494:Protein Kinases