Versatile properties of an exopolysaccharide R-PS18 produced by Rhizobium sp. PRIM-18.
Exopolysaccharides (EPS) produced by bacteria have attracted scientific and industrial attention due to their multifunctional properties and relatively easier production. In this study, an EPS viz., R-PS18 produced by Rhizobium sp. PRIM-18 was characterized and its functional properties were assessed. Cell proliferative and in vitro wound healing activities of the EPS were established using human dermal fibroblast (HDF) cells. The isolate produced 2.1 g L(-1) purified EPS (molecular weight 9.33×10(6) Da) comprising of glucose, galactose, and mannose (6.1:1.8:1). Viscosity of 0.25% solution was 23.4 mPa s (shear rate 75 s(-1)) and it showed pseudoplastic and thixotropic behavior. High emulsification, iron chelation, and superoxide scavenging abilities were also observed. Significant increase in HDF cell proliferation and wound healing in vitro was achieved by R-PS18 treatment. Sulfation of R-PS18 significantly enhanced the cell proliferative and wound healing activities. In conclusion, these findings indicate potential applications of R-PS18.
Yenepoya Research Centre, Yenepoya University, Mangalore, India.
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Priyanka
Yenepoya Research Centre, Yenepoya University, Mangalore, India.
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Arun
Yenepoya Research Centre, Yenepoya University, Mangalore, India.
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Ashwini
Yenepoya Research Centre, Yenepoya University, Mangalore, India. Electronic address: rekhapd@hotmail.com.
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Rekha
D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D045703:Emulsifying Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D012231:Rhizobium / Q000737:chemistry*; D014783:Viscosity; D014945:Wound Healing / Q000187:drug effects
D000975:Antioxidants; D045703:Emulsifying Agents; D011135:Polysaccharides, Bacterial
Cell proliferation; Emulsification; Exopolysaccharide; Rhizobium sp.; Wound healing