Transport characteristics and morphology of the colon and coprodeum in two wild birds of different habitats, the rock ptarmigan (Lagopus mutus) and the common murre (Uria aalge).
Dietary salt intake in domestic fowl affects epithelial transport and morphology of the lower intestine (colon and coprodeum). This study investigated lower intestinal morphology and transport activity in two wild bird species with natural diets containing either low or high salt. Tissues from rock ptarmigan (Lagopus mutus) and common murres (Uria aalge) were sampled for histology and electrophysiological analyses. The ptarmigan exists on a low salt diet, while the murre lives on a high protein and high salt diet. The ptarmigan colon and coprodeum had villi/folds and crypts and the epithelium contained absorptive epithelial cells, mitochondria-rich cells and goblet cells. The colon had significant amiloride-inhibitable Isc, 5-15 μA/cm(2), with no glucose-stimulated Isc, and no significant phloridzin inhibition. The coprodeum also had high amiloride-inhibitable Isc. This transport pattern corresponded to that of chickens on low-salt diets. However, the ptarmigan colon also had a significant lysine/leucine-stimulated Isc of 3±1.0 μA/cm(2). The short U. aalge colon was similar to that of ptarmigans, but with no villi. It demonstrated a significant lysine/leucine-stimulated Isc (11±3.5 μA/cm(2)) with no amiloride-inhibitable Isc, similar to the high-salt chicken colon, but with no Na(+)-glucose cotransport. The murre coprodeum was inert to all substances and showed high resistance (1000 Ω·cm(2)), with a multilayered squamous epithelium. Despite some variations possibly associated with dietary protein intake, we conclude that natural high and low salt diets in different avian species are associated with different lower intestinal transport patterns, providing for post-renal adjustments in ion and water excretion.
Department of Physiology, University of Iceland, Reykjavík, Iceland.
Sighvatur S
SS
Árnason
Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, Copenhagen, Denmark.
Vibeke S
VS
Elbrønd
Department of Biological Sciences, University of DE, Newark, USA. Electronic address: Laverty@udel.edu.
Gary
G
Laverty
D000818:Animals; D001717:Birds / Q000033:anatomy & histology / Q000502:physiology*; D003106:Colon / Q000033:anatomy & histology / Q000502:physiology*; D017753:Ecosystem; D013045:Species Specificity
Amiloride; Colon; Coprodeum; Epithelial morphology; High/low salt diets; Lagopus mutus; Na(+)/amino acid cotransport; Na(+)/glucose cotransport; Uria aalge