The LPS-Pretreated MSCs Supply a Positive Microenvironment for Tumor Cell Proliferation and Clone Formation.
In this study, the culture supernatant of LPS-treated MSCs was collected and served as a conditioned medium (CM). It was found that the LPS-CM promoted the proliferation of tumor cells (SKBR3, MDA-MB-231, A549, 95D and HepG2). In addition, the colony formation ability was also enhanced by LPS-CM incubation. The inflammatory cytokines, IL-6 and IL-8, were demonstrated to be up-regulated in the LPS-CM, which we supposed to function in the tumor-growth promotion in vitro.
Pengfei
P
Li
Jin
J
Liu
Department of Hematology and Oncology, The First Affiliated Hospital of General Hospital of Chinese People's Liberation Army (PLA), No. 52，Fucheng Road, Haidian District, Beijing 100039, China. sjunzh07@163.com.
Junzhong
J
Sun
Haifeng
H
Duan
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D059016:Tumor Microenvironment / Q000187:drug effects*; D015854:Up-Regulation / Q000187:drug effects
D015850:Interleukin-6; D016209:Interleukin-8; D008070:Lipopolysaccharides; D016328:NF-kappa B