Chlorogenic Acid Biosynthesis Appears Linked with Suberin Production in Potato Tuber (Solanum tuberosum).
Potato (Solanum tuberosum L.) is a good source of dietary antioxidants. Chlorogenic acid (CGA) and caffeic acid (CA) are the most abundant phenolic acid antioxidants in potato and are formed by the phenylpropanoid pathway. A number of CGA biosynthetic routes that involve hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase (HQT) and/or hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase (HCT) have been proposed, but little is known about their path in potato. CA production requires a caffeoyl shikimate esterase (CSE), and CA serves as a substrate of lignin precursor ferulic acid via the action of caffeic/5-hydroxyferulic acid O-methyltransferase (COMT I). CGA is precursor of caffeoyl-CoA and, via caffeoyl-CoA O-methyltransferase (CCoAOMT), of feruloyl-CoA. Feruloyl-CoA is required for lignin and suberin biosynthesis, crucial for tuber development. Here, metabolite and transcript levels of the mentioned and related enzymes, such as cinnamate 4-hydroxylase (C4H), were determined in the flesh and skin of fresh and stored tubers. Metabolite and transcript levels were higher in skin than in flesh, irrespective of storage. CGA and CA production appear to occur via p-coumaroyl-CoA, using HQT and CSE, respectively. HCT is likely involved in CGA remobilization toward suberin. The strong correlation between CGA and CA, the correspondence with C4H, HQT, CCoAOMT2, and CSE, and the negative correlation of HCT and COMT I in potato tubers suggest a major flux toward suberin.
Instituto de Investigaciones Biológicas-CONICET, Universidad Nacional de Mar del Plata, CC 1245, 7600 Mar del Plata, Argentina.
Matías Ariel
MA
Valiñas
Instituto de Investigaciones Biológicas-CONICET, Universidad Nacional de Mar del Plata, CC 1245, 7600 Mar del Plata, Argentina.
María Luciana
ML
Lanteri
Instituto de Investigaciones Biológicas-CONICET, Universidad Nacional de Mar del Plata, CC 1245, 7600 Mar del Plata, Argentina.
Arjen
A
ten Have
Instituto de Investigaciones Biológicas-CONICET, Universidad Nacional de Mar del Plata, CC 1245, 7600 Mar del Plata, Argentina.
Adriana Balbina
AB
Andreu
D000214:Acyl Coenzyme A / Q000235:genetics / Q000378:metabolism; D053898:Biosynthetic Pathways; D002726:Chlorogenic Acid / Q000378:metabolism*; D008055:Lipids / Q000096:biosynthesis*; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D035281:Plant Tubers / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D011198:Solanum tuberosum / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D050564:Trans-Cinnamate 4-Monooxygenase / Q000235:genetics / Q000378:metabolism
D000214:Acyl Coenzyme A; D008055:Lipids; D010940:Plant Proteins; D002726:Chlorogenic Acid; C058645:caffeoyl-coenzyme A; C065875:suberin; D050564:Trans-Cinnamate 4-Monooxygenase; D008780:Methyltransferases; C059825:caffeoyl-CoA O-methyltransferase; C019608:caffeate O-methyltransferase
Solanum tuberosum; antioxidant; caffeic acid; flavonoid metabolism; phenolic acids metabolism; storage