Emergence of novel clade 2.3.4 influenza A (H5N1) virus subgroups in Yunnan Province, China.
From December 2013 to March 2014, a major wave of highly pathogenic avian influenza outbreak occurred in poultry in Yunnan Province, China. We isolated and characterized eight highly pathogenic avian influenza A (H5N1) viruses from poultry. Full genome influenza sequences and analyses have been performed. Sequence analyses revealed that they belonged to clade 2.3.4 but did not fit within the three defined subclades. The isolated viruses were provisional subclade 2.3.4.4e. The provisional subclade 2.3.4.4e viruses with six internal genes from avian influenza A (H5N2) viruses in 2013 were the novel reassortant influenza A (H5N1) viruses which were associated with the outbreak of H5N1 occurred in egg chicken farms in Yunnan Province. The HA genes were similar to subtype H5 viruses isolated from January to March of 2014 in Asia including H5N6 and H5N8. The NA genes were most closely related to A/chicken/Vietnam/NCVD-KA423/2013 (H5N1) from the subclade 2.3.2. The HI assay demonstrated a lack of antigenic relatedness between clades 2.3.4.4e and 2.3.4.1 (RE-5 vaccine strain) or 2.3.2.2 (RE-6 vaccine strain).
Centre for Disease Control and Prevention, Chengdu Military Region, Kunming 650118, China.
Tingsong
T
Hu
Yunnan Key Laboratory of Tropical and Subtropical Animal Virus Diseases, Kunming 650224, China.
Jianling
J
Song
Centre for Animal Disease Control and Prevention, Kunming 650051, Yunnan Province, China.
Wendong
W
Zhang
Centre for Animal Disease Control and Prevention, Kunming 650051, Yunnan Province, China.
Huanyun
H
Zhao
Centre for Animal Disease Control and Prevention, Kunming 650051, Yunnan Province, China.
Bofang
B
Duan
Centre for Animal Disease Control and Prevention, Kunming 650051, Yunnan Province, China; Yunnan Agriculture University, Kunming 650223, China.
Qingliang
Q
Liu
Centre for Animal Disease Control and Prevention, Kunming 650051, Yunnan Province, China; Yunnan Agriculture University, Kunming 650223, China.
Wei
W
Zeng
Centre for Disease Control and Prevention, Chengdu Military Region, Kunming 650118, China.
Wei
W
Qiu
Centre for Disease Control and Prevention, Chengdu Military Region, Kunming 650118, China.
Gang
G
Chen
Centre for Animal Disease Control and Prevention, Kunming 650051, Yunnan Province, China.
Yingguo
Y
Zhang
Centre for Disease Control and Prevention, Chengdu Military Region, Kunming 650118, China. Electronic address: fqs168@126.com.
Quanshui
Q
Fan
Centre for Disease Control and Prevention, Chengdu Military Region, Kunming 650118, China. Electronic address: zfq1968@aliyun.com.
Fuqiang
F
Zhang
D002681:China / Q000453:epidemiology; D021821:Communicable Diseases, Emerging; D005838:Genotype; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000145:classification* / Q000235:genetics*; D007251:Influenza, Human / Q000453:epidemiology* / Q000821:virology*; D009154:Mutation; D009439:Neuraminidase / Q000235:genetics; D010802:Phylogeny; D014764:Viral Proteins / Q000235:genetics
D019267:Hemagglutinin Glycoproteins, Influenza Virus; D014764:Viral Proteins; D009439:Neuraminidase
Clade 2.3.4; H5N1; Highly pathogenic avian influenza