Sodium houttuyfonate inhibits inflammation by blocking the MAPKs/NF-κB signaling pathways in bovine endometrial epithelial cells.
Sodium houttuyfonate (SH) has traditionally been used for the therapy of inflammatory diseases. In this research, we tried to assess the anti-inflammatory effects of SH on LPS-induced bovine endometrial epithelial cell (bEEC) inflammation. SH cell toxicity was measured using the MTT and LDH assays, and inflammatory cytokine expression was assessed by ELISA, qRT-PCR and Western blotting. We demonstrated that SH was not cytotoxic to bEECs, and that it significantly decreased the LPS-induced mRNA and protein expression of tumor necrosis factor (TNF) α, interleukin (IL)-1β, IL-6 and IL-8. Furthermore, in LPS-induced bEECs, SH inhibited IκBα degradation and NF-κB p65 phosphorylation, and suppressed the phosphorylation of the mitogen-activated protein kinases (MAPKs), p38, c-Jun N-terminal kinase (JNK), and extracellular signal-regulated kinase (ERK). In conclusion, we found that SH could effectively block the NF-κB-mediated signaling pathway and reduce the inflammatory process, thereby exerting a protective effect on bEECs.
College of Veterinary Medicine, China Agricultural University (CAU), Beijing 100193, China.
Qi
Q
Zhu
College of Veterinary Medicine, China Agricultural University (CAU), Beijing 100193, China.
Xiaolong
X
Xu
College of Veterinary Medicine, China Agricultural University (CAU), Beijing 100193, China.
Xiaoxi
X
Liu
College of Veterinary Medicine, China Agricultural University (CAU), Beijing 100193, China.
Jiabao
J
Lin
College of Veterinary Medicine, China Agricultural University (CAU), Beijing 100193, China.
Yao
Y
Kan
College of Veterinary Medicine, China Agricultural University (CAU), Beijing 100193, China.
Yougang
Y
Zhong
Beijing Key Laboratory for Dairy Cow Nutrition, College of Animal Science and Technology, Beijing University of Agriculture (BUA), Beijing 102206, China. Electronic address: liufenghua1209@126.com.
Fenghua
F
Liu
College of Veterinary Medicine, China Agricultural University (CAU), Beijing 100193, China. Electronic address: xujianqincau@126.com.
Jianqin
J
Xu
D000473:Alkanes / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002417:Cattle; D002418:Cattle Diseases / Q000188:drug therapy / Q000276:immunology; D018925:Chemokines / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism*; D004716:Endometritis / Q000188:drug therapy / Q000276:immunology / Q000662:veterinary*; D004717:Endometrium / Q000187:drug effects / Q000276:immunology; D004847:Epithelial Cells / Q000187:drug effects / Q000276:immunology; D005260:Female; D007249:Inflammation / Q000188:drug therapy / Q000276:immunology / Q000662:veterinary*; D020935:MAP Kinase Signaling System / Q000187:drug effects; D016328:NF-kappa B / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D013447:Sulfites / Q000494:pharmacology*
D000473:Alkanes; D000893:Anti-Inflammatory Agents; D018925:Chemokines; D016207:Cytokines; D016328:NF-kappa B; D013447:Sulfites; C473296:sodium houttuyfonate
Bovine endometrial epithelial cells; Inflammation; Lipopolysaccharide; MAPKs; NF-κB; Sodium houttuyfonate