Cross-reactivity of Toxocariasis with Crude Antigen of Toxascaris leonina Larvae by ELISA.
Roundworms of Toxocara canis and Toxascaris leonina are common gastrointestinal helminths of canids over the world. Humans are infected with T. canis larvae through ingestion of infective eggs in contaminated environments or larvae by consumption of raw or uncooked meat or livers. Recently, patients of clinically diagnosed toxocariasis are increasing and require correct diagnosis in Korea. The present study investigated serological cross-reactivity between crude antigens of T. canis (TCLA) and T. leonina (TLLA) larvae. We collected serum specimens from 177 toxocariasis patients who were clinically suspected in the Seoul National University Hospital and 115 healthy controls. An ELISA method for toxocariasis was used to evaluate diagnostic efficacy of TLLA for serodiagnosis of human toxocariasis. The IgG ELISA using TLLA gave 14 (14.3%) positives of 98 TCLA positive specimens among 177 suspected toxocariasis patients. Most of them showed high absorbances with TCLA. In conclusion, there is a partial cross reaction between serum specimens of toxocariasis and TLLA.
Department of Parasitology and Tropical Medicine, Institute of Endemic Diseases, Seoul National University College of Medicine, Seoul, Korea.
Yan
0000-0002-6118-2069
Y
Jin
Department of Microbiology, Qingdao University College of Medicine, Qingdao, China.
Chenghua
0000-0001-9906-3738
C
Shen
Department of Parasitology, Hallym University, College of Medicine, Chuncheon, Korea.
Sun
0000-0002-8559-8640
S
Huh
Department of Parasitology and Tropical Medicine, Institute of Endemic Diseases, Seoul National University College of Medicine, Seoul, Korea.
Min-Ho
0000-0002-0130-2815
MH
Choi
Department of Parasitology and Tropical Medicine, Institute of Endemic Diseases, Seoul National University College of Medicine, Seoul, Korea.
Sung-Tae
0000-0002-0300-1944
ST
Hong
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000276:immunology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007814:Larva / Q000276:immunology / Q000378:metabolism; D014114:Toxascaris / Q000254:growth & development / Q000276:immunology* / Q000302:isolation & purification; D017166:Toxocara canis / Q000254:growth & development / Q000276:immunology* / Q000302:isolation & purification; D014120:Toxocariasis / Q000175:diagnosis* / Q000469:parasitology
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G
Cross-reaction; Crude Antigen; Larvae; Serodiagnosis; Serum IgG; Toxascaris leonina; Toxocara canis; Toxocariasis