A hot water extract of Curcuma longa inhibits adhesion molecule protein expression and monocyte adhesion to TNF-α-stimulated human endothelial cells.
The recruitment of arterial leukocytes to endothelial cells is an important step in the progression of various inflammatory diseases. Therefore, its modulation is thought to be a prospective target for the prevention or treatment of such diseases. Adhesion molecules on endothelial cells are induced by proinflammatory cytokines, including tumor necrosis factor-α (TNF-α), and contribute to the recruitment of leukocytes. In the present study, we investigated the effect of hot water extract of Curcuma longa (WEC) on the protein expression of adhesion molecules, monocyte adhesion induced by TNF-α in human umbilical vascular endothelial cells (HUVECs). Treatment of HUVECs with WEC significantly suppressed both TNF-α-induced protein expression of adhesion molecules and monocyte adhesion. WEC also suppressed phosphorylation and degradation of nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IκBα) induced by TNF-α in HUVECs, suggesting that WEC inhibits the NF-κB signaling pathway.
a Research & Development Institute , House Wellness Foods Corporation , Itami , Japan.
Kengo
K
Kawasaki
Koutarou
K
Muroyama
Norio
N
Yamamoto
Shinji
S
Murosaki
D002448:Cell Adhesion / Q000187:drug effects; D030024:Curcuma / Q000737:chemistry*; D019040:E-Selectin / Q000235:genetics / Q000276:immunology; D005786:Gene Expression Regulation; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / Q000235:genetics / Q000276:immunology; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D020672:I-kappa B Proteins / Q000235:genetics / Q000276:immunology; D007155:Immunologic Factors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000276:immunology; D000072000:NF-KappaB Inhibitor alpha; D010936:Plant Extracts / Q000737:chemistry; D015398:Signal Transduction; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics / Q000276:immunology; D014867:Water
D019040:E-Selectin; D020672:I-kappa B Proteins; D007155:Immunologic Factors; C000606204:NFKBIA protein, human; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1; D014867:Water; D018799:Intercellular Adhesion Molecule-1; D000072000:NF-KappaB Inhibitor alpha; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)
Curcuma longa; NF-κB; human umbilical vascular endothelial cells (HUVECs); tumor necrosis factor-α (TNF-α); vascular cell adhesion molecule-1