Oxidative stress, genotoxicity and histopathology biomarker responses in Mugil cephalus and Dicentrarchus labrax gill exposed to persistent pollutants. A field study in the Bizerte Lagoon: Tunisia.
The use of biomarkers has become an important tool for modern environmental assessment as they can help to predict pollutants involved in the monitoring program. Despite the importance of fish gill in several functions (gaseous exchange, osmotic and ionic regulation, acid-base balance and nitrogenous waste) its use in coastal water biomonitoring focusing on protection and damage is scarce. This field study investigates biochemical (catalase, superoxide dismutase, lipid peroxidation), molecular (DNA integrity) and morphological (histology) parameters in gill of mullet (Mugil cephalus) and sea bass (Dicentrarchus labrax) and originating from Bizerte lagoon (a coastal lagoon impacted by different anthropogenic activities) and from the Mediterranean Sea (a reference site). Remarkable alterations in the activities of oxidative stress enzymes and DNA integrity in the tissue of the two studied fish species were detected in Bizerte Lagoon. The study of histopathological alterations of gills in both two fish species from Bizerte Lagoon suggest thickening of primary lamellae, cellular hyperplasia, aneurism, curving, shortening and fusion of secondary lamellae. The adopted approach, considering simultaneously protection responses and damaging effects, revealed its usefulness on the pollution assessment.
Laboratory of Heteroatom Organic Chemistry, Department of Chemistry, Faculty of Sciences of Bizerte, University of Carthage, 7021 Zarzouna, Tunisia.
Walid Ben
WB
Ameur
Laboratory of Heteroatom Organic Chemistry, Department of Chemistry, Faculty of Sciences of Bizerte, University of Carthage, 7021 Zarzouna, Tunisia.
Yassine
Y
El Megdiche
UTOX-PCB, Unit of Experimental Toxicology and Ecotoxicology, Parc Científic Barcelona, Barcelona, Spain. Electronic address: jlapuente@pcb.ub.cat.
Joaquin
J
de Lapuente
Laboratory of Heteroatom Organic Chemistry, Department of Chemistry, Faculty of Sciences of Bizerte, University of Carthage, 7021 Zarzouna, Tunisia.
Badreddine
B
Barhoumi
Laboratory of Heteroatom Organic Chemistry, Department of Chemistry, Faculty of Sciences of Bizerte, University of Carthage, 7021 Zarzouna, Tunisia.
Souad
S
Trabelsi
Laboratory of Heteroatom Organic Chemistry, Department of Chemistry, Faculty of Sciences of Bizerte, University of Carthage, 7021 Zarzouna, Tunisia.
Soukaina
S
Ennaceur
UTOX-PCB, Unit of Experimental Toxicology and Ecotoxicology, Parc Científic Barcelona, Barcelona, Spain.
Lydia
L
Camps
UTOX-PCB, Unit of Experimental Toxicology and Ecotoxicology, Parc Científic Barcelona, Barcelona, Spain.
Joan
J
Serret
UTOX-PCB, Unit of Experimental Toxicology and Ecotoxicology, Parc Científic Barcelona, Barcelona, Spain.
David
D
Ramos-López
UTOX-PCB, Unit of Experimental Toxicology and Ecotoxicology, Parc Científic Barcelona, Barcelona, Spain.
Javier
J
Gonzalez-Linares
Laboratory of Heteroatom Organic Chemistry, Department of Chemistry, Faculty of Sciences of Bizerte, University of Carthage, 7021 Zarzouna, Tunisia.
Soufiane
S
Touil
Laboratory of Heteroatom Organic Chemistry, Department of Chemistry, Faculty of Sciences of Bizerte, University of Carthage, 7021 Zarzouna, Tunisia.
Mohamed Ridha
MR
Driss
UTOX-PCB, Unit of Experimental Toxicology and Ecotoxicology, Parc Científic Barcelona, Barcelona, Spain.
Miquel
M
Borràs
D000818:Animals; D001492:Bass / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D004249:DNA Damage; D004784:Environmental Monitoring; D005399:Fishes / Q000502:physiology*; D005880:Gills / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008522:Mediterranean Sea; D018384:Oxidative Stress; D023701:Smegmamorpha / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D014416:Tunisia; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
D015415:Biomarkers; D014874:Water Pollutants, Chemical; D002374:Catalase; D013482:Superoxide Dismutase
Bizerte Lagoon; Fish gill; Genotoxicity; Histopathology; Oxidative stress; Pollution