One-step purification of R-phycoerythrin from the red edible seaweed Grateloupia turuturu.
A one-step chromatographic method for the purification of R-phycoerythrin (R-PE) of Grateloupia turuturu Yamada is described. Native R-PE was obtained with a purity index of 2.89 and a recovery yield of 27% using DEAE-Sepharose Fast Flow chromatography with a three-step increase in ionic strength. The analysis by SDS electrophoresis showed a broad band between 18 and 21kDa in size corresponding to subunits α and β and a low intensity band of 29kDa corresponding to the γ subunit. Two forms of R-PE were identified by gel filtration chromatography: a native form with a molecular weight of 260±5kDa and a dissociated form with a molecular weight of 60±2kDa. The native form presented the characteristic absorption spectrum of R-PE with three absorbance maxima at 498, 540 and 565nm, whereas the dissociated form presented only the 498 and 540nm peaks. Moreover, the two forms displayed two different fluorescence maxima.
FR CNRS 3473 IUML, Mer Molécule Santé (MMS), EA 2160, Université de Nantes, 2 rue de la Houssinière, BP 92208, 44322 Nantes Cedex 3, France.
Mathilde
M
Munier
FR CNRS 3473 IUML, Mer Molécule Santé (MMS), EA 2160, Université de Nantes, 2 rue de la Houssinière, BP 92208, 44322 Nantes Cedex 3, France.
Michèle
M
Morançais
FR CNRS 3473 IUML, Mer Molécule Santé (MMS), EA 2160, Université de Nantes, 2 rue de la Houssinière, BP 92208, 44322 Nantes Cedex 3, France.
Justine
J
Dumay
FR CNRS 3473 IUML, UMR-CNRS 6144 (GEPEA), Université de Nantes, CRTT 37 boulevard de l'Université, BP 406, 44602 Saint-Nazaire Cedex, France.
Pascal
P
Jaouen
FR CNRS 3473 IUML, Mer Molécule Santé (MMS), EA 2160, Université de Nantes, 2 rue de la Houssinière, BP 92208, 44322 Nantes Cedex 3, France. Electronic address: joel.fleurence@univ-nantes.fr.
Joël
J
Fleurence
D002852:Chromatography, Ion Exchange / Q000379:methods; D010799:Phycoerythrin / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry*; D000461:Rhodophyta / Q000737:chemistry*
D010936:Plant Extracts; D010799:Phycoerythrin
Grateloupia turuturu; Ion-exchange chromatography; Purification; R-phycoerythrin