Insight into the genomic diversity and relationship of Astragalus glycyphyllos symbionts by RAPD, ERIC-PCR, and AFLP fingerprinting.
We assessed the genomic diversity and genomic relationship of 28 Astragalus glycyphyllos symbionts by three methodologies based on PCR reaction, i.e., RAPD, ERIC-PCR, and AFLP. The AFLP method with one PstI restriction enzyme and selective PstI-GC primer pair had a comparable discriminatory power as ERIC-PCR one and these fingerprinting techniques distinguished among the studied 28 A. glycyphyllos symbionts 18 and 17 genomotypes, respectively. RAPD method was less discriminatory in the genomotyping of rhizobia analyzed and it efficiently resolved nine genomotypes. The cluster analysis of RAPD, ERIC-PCR, and AFLP profiles resulted in a generally similar grouping of the test strains on generated dendrograms supporting a great potential of these DNA fingerprinting techniques for study of genomic polymorphism and evolutionary relationship of A. glycyphyllos nodulators. The RAPD, ERIC-PCR, and AFLP pattern similarity coefficients between A. glycyphyllos symbionts studied was in the ranges 8-100, 18-100, and 23-100%, respectively.
Department of Veterinary Microbiology, University of Life Sciences, 12 Akademicka st., 20-950, Lublin, Poland.
Sebastian
S
Gnat
Department of Genetics and Microbiology, University of Maria Curie-Skłodowska, 19 Akademicka st., 20-033, Lublin, Poland. wanda.malek@poczta.umcs.lublin.pl.
Wanda
W
Małek
Department of Genetics and Evolution, Institute of Biology, University of Białystok, 20B Świerkowa st., 15-950, Białystok, Poland.
Ewa
E
Oleńska
Department of Veterinary Microbiology, University of Life Sciences, 12 Akademicka st., 20-950, Lublin, Poland.
Aleksandra
A
Trościańczyk
Department of Genetics and Microbiology, University of Maria Curie-Skłodowska, 19 Akademicka st., 20-033, Lublin, Poland.
Sylwia
S
Wdowiak-Wróbel
Department of Genetics and Microbiology, University of Maria Curie-Skłodowska, 19 Akademicka st., 20-033, Lublin, Poland.
Michał
M
Kalita
Department of Genetics and Microbiology, University of Maria Curie-Skłodowska, 19 Akademicka st., 20-033, Lublin, Poland.
Magdalena
M
Wójcik
D054458:Amplified Fragment Length Polymorphism Analysis; D027883:Astragalus Plant / Q000382:microbiology*; D001419:Bacteria / Q000145:classification*; D016172:DNA Fingerprinting / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D053204:Root Nodules, Plant / Q000382:microbiology; D013559:Symbiosis
D004269:DNA, Bacterial
Astragalus glycyphyllos symbionts; DNA fingerprinting; Genome polymorphism; Genomic relationship; Rhizobia