Enhancing repair of full-thickness excisional wounds in a murine model: Impact of tissue-engineered biological dressings featuring human differentiated adipocytes.
Promotion of skin repair for acute or chronic wounds through the use of tissue-engineered products is an active field of research. This study evaluates the effects mediated by tissue-engineered biological dressings containing human in vitro-differentiated adipocytes and adipose-derived stromal cells (ASCs). Re-epithelialization, granulation tissue formation and neovascularization of full-thickness cutaneous wounds were specifically assessed using a murine model featuring a fluorescent epidermis. In comparison with wounds that did not receive an adipocyte-containing biological dressing, treated wounds displayed a slight but significantly faster wound closure based on macroscopic observations over 18 days. Non-invasive imaging of GFP-expressing keratinocytes determined that the kinetics of re-epithelialization were similar for both groups. Treated wounds featured thicker granulation tissues (1.7-fold, P < 0.0001) enriched in collagens (1.3-fold, P < 0.0104). In addition, wound cryosections labeled for detection of CD31-expressing cells indicated a 2.2-fold (P < 0.0002) increased neovascularization for the treated wounds at the time of terminal biopsy. This is in accordance with the secretion of pro-angiogenic factors detected in media conditioned by the dressings. Taken together, these results establish that a new type of engineered substitutes featuring a mixture of adipocytes and ASCs can promote cutaneous healing when applied as temporary dressings, suggesting their potential relevance for chronic wound management studies.
Laval University Tissue Engineering Laboratory/LOEX, Québec, QC, Canada; Division of regenerative medicine, CHU de Quebec Research Centre, Québec, QC, Canada. Electronic address: pascal.morissette-martin.1@ulaval.ca.
Pascal
P
Morissette Martin
Laval University Tissue Engineering Laboratory/LOEX, Québec, QC, Canada; Division of regenerative medicine, CHU de Quebec Research Centre, Québec, QC, Canada. Electronic address: am.maux@gmail.com.
Amandine
A
Maux
Laval University Tissue Engineering Laboratory/LOEX, Québec, QC, Canada; Division of regenerative medicine, CHU de Quebec Research Centre, Québec, QC, Canada; Department of Mechanical Engineering, Faculty of Sciences and Engineering, Laval University, Québec, QC, Canada. Electronic address: veronique.laterreur.1@ulaval.ca.
Véronique
V
Laterreur
Laval University Tissue Engineering Laboratory/LOEX, Québec, QC, Canada; Division of regenerative medicine, CHU de Quebec Research Centre, Québec, QC, Canada. Electronic address: dominique.mayrand.1@ulaval.ca.
Dominique
D
Mayrand
Laval University Tissue Engineering Laboratory/LOEX, Québec, QC, Canada; Division of regenerative medicine, CHU de Quebec Research Centre, Québec, QC, Canada. Electronic address: valerie.l-gagne.1@ulaval.ca.
Valérie
V
L Gagné
Laval University Tissue Engineering Laboratory/LOEX, Québec, QC, Canada; Division of regenerative medicine, CHU de Quebec Research Centre, Québec, QC, Canada; Department of Surgery, Faculty of Medicine, Laval University, Québec, QC, Canada. Electronic address: veronique.moulin@chg.ulaval.ca.
Véronique J
VJ
Moulin
Laval University Tissue Engineering Laboratory/LOEX, Québec, QC, Canada; Division of regenerative medicine, CHU de Quebec Research Centre, Québec, QC, Canada; Department of Surgery, Faculty of Medicine, Laval University, Québec, QC, Canada. Electronic address: julie.fradette@chg.ulaval.ca.
Julie
J
Fradette
D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects; D000328:Adult; D000818:Animals; D001684:Biological Dressings; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004195:Disease Models, Animal; D004848:Epithelium / Q000187:drug effects; D005260:Female; D006097:Granulation Tissue / Q000187:drug effects / Q000473:pathology; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology; D007700:Kinetics; D051379:Mice; D018919:Neovascularization, Physiologic / Q000187:drug effects; D023822:Tissue Engineering / Q000379:methods*; D014945:Wound Healing / Q000187:drug effects*
D036341:Intercellular Signaling Peptides and Proteins
Adipocytes; Adipose-derived stem cells; Skin; Tissue engineering; Wound healing