TLR9-deficiency reduces TLR1, TLR2 and TLR3 expressions in Leishmania major-infected macrophages.
The parasite Leishmania major counteractively modulates TLR2 and TLR9 expression and their functions. Although TLR1, TLR3, TLR4, and TLR7 are also implicated in Leishmania infection, whether their expression was altered in TLR2 or TLR9 deficiency remained unknown. Therefore, we examined TLR1, TLR3, TLR4 and TLR7 expression in L. major infection in TLR2-deficient or TLR9-deficient macrophages. We observed that TLR9-deficiency reduced TLR1, TLR2 and TLR3 but not TLR7 expression in the macrophages treated with live or killed L. major promastigotes. TLR2-deficiency had little effects by comparison. TLR9-deficient macrophages had reduced CD40 expression and less IL-12 and TNF-α expression. Thus, we report that TLR9 modulates TLR1, TLR2 and TLR3, but not TLR7, expression in L. major-infected macrophages.
National Centre for Cell Science, Ganeshkhind, Pune 411007, India. Electronic address: surya.bt@gmail.com.
Surya Prakash
SP
Pandey
Pasteur Institute, 25-28 Rue de Docteur, Paris, France.
Noelle
N
Doyen
National Centre for Cell Science, Ganeshkhind, Pune 411007, India.
Gyan C
GC
Mishra
National Centre for Cell Science, Ganeshkhind, Pune 411007, India.
Bhaskar
B
Saha
National Centre for Cell Science, Ganeshkhind, Pune 411007, India.
Himanshu Singh
HS
Chandel
D000818:Animals; D019013:CD40 Antigens / Q000235:genetics / Q000378:metabolism; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D016054:DNA, Protozoan / Q000378:metabolism; D018320:Leishmania major / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D016773:Leishmaniasis, Cutaneous / Q000276:immunology*; D017737:Macrophages, Peritoneal / Q000276:immunology / Q000378:metabolism / Q000469:parasitology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013047:Specific Pathogen-Free Organisms; D051194:Toll-Like Receptor 1 / Q000235:genetics / Q000378:metabolism; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000378:metabolism; D051196:Toll-Like Receptor 3 / Q000235:genetics / Q000378:metabolism; D051217:Toll-Like Receptor 9 / Q000172:deficiency*; D051193:Toll-Like Receptors / Q000235:genetics / Q000378:metabolism*; D059467:Transcriptome
D019013:CD40 Antigens; D016207:Cytokines; D016054:DNA, Protozoan; C519703:TLR3 protein, mouse; C493486:Tlr2 protein, mouse; C495370:Tlr9 protein, mouse; D051194:Toll-Like Receptor 1; D051195:Toll-Like Receptor 2; D051196:Toll-Like Receptor 3; D051217:Toll-Like Receptor 9; D051193:Toll-Like Receptors
CD40; Cytokines; Leishmania; TLR expression; TLR2; TLR9