Metabolic Response of Escherichia coli upon Treatment with Hypochlorite at Sub-Lethal Concentrations.
Hypochlorite is a reactive oxygen species that is worldwide as an antibacterial disinfectant. Hypochlorite exposure is known to cause oxidative damage to DNA and proteins. As a response to these effects, the metabolite profiles of organisms treated with sub-lethal doses of hypochlorite are assumed to be severely modified; however, the nature of these changes is hardly understood. Therefore, using nuclear magnetic resonance spectroscopy and gas chromatography-coupled mass spectrometry, we analyzed the time-dependent impact of hypochlorite exposure with a sub-lethal concentration (50 µM) on the metabolite profile of the Escherichia coli strain MG1655. Principle component analysis clearly distinguished between the metabolite profiles of bacteria treated for 0, 5, 10, 20, 40, or 60 min. Major changes in the relative amounts of fatty acids, acetic acid, and formic acid occurred within the first 5 min. Comparative gas chromatography-coupled mass spectrometry analyses revealed that the amounts of free methionine and alanine were significantly decreased in the treated cells, demonstrating their susceptibility to hypochlorite exposure. The concentrations of succinate, urea, orotic acid, 2-aminobutyric acid, and 2-hydroxybutyric acid were also severely affected, indicating general changes in the metabolic network by hypochlorite. However, most metabolite levels relaxed to the reference values of untreated cells after 40-60 min, reflecting the capability of E. coli to rapidly adapt to environmental stress factors such as the presence of sub-lethal oxidant levels.
Center for Integrated Protein Science Munich (CiPSM), Lehrstuhl für Biotechnologie, Technische Universität München, Lichtenbergstr. 4, Garching, Germany.
Adrian
A
Drazic
Lehrstuhl für Biochemie, Technische Universität München, Lichtenbergstr. 4, Garching, Germany.
Erika
E
Kutzner
Center for Integrated Protein Science Munich (CiPSM), Lehrstuhl für Biotechnologie, Technische Universität München, Lichtenbergstr. 4, Garching, Germany.
Jeannette
J
Winter
Lehrstuhl für Biochemie, Technische Universität München, Lichtenbergstr. 4, Garching, Germany.
Wolfgang
W
Eisenreich
D000596:Amino Acids / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006997:Hypochlorous Acid / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D055442:Metabolome / Q000187:drug effects; D055432:Metabolomics; D025341:Principal Component Analysis; D066244:Proton Magnetic Resonance Spectroscopy; D012997:Solvents; D013312:Stress, Physiological / Q000187:drug effects; D013997:Time Factors
D000596:Amino Acids; D005227:Fatty Acids; D012997:Solvents; D006997:Hypochlorous Acid