In Situ Electrochemical ELISA for Specific Identification of Captured Cancer Cells.
Circulating tumor cells (CTCs) are cancer cells disseminated from a tumor into the bloodstream. Their presence in patient blood samples has been associated with metastatic disease. Here, we report a simple system that enables the isolation and detection of these rare cancer cells. By developing a sensitive electrochemical ELISA method integrated within a microfluidic cell capture system, were we able to reliably detect very low levels of cancer cells in whole blood. Our results indicate that the new system provides the clinically relevant specificity and sensitivity needed for a convenient, point-of-need assay for cancer cell counting.
†Department of Electrical and Computer Engineering, Faculty of Applied Science and Engineering, University of Toronto, 10 King's College Road, Toronto, Ontario M5S 3G4, Canada.
Tina Saberi
TS
Safaei
‡Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto, 144 College Street, Toronto, Ontario M5S 3M2, Canada.
Reza M
RM
Mohamadi
†Department of Electrical and Computer Engineering, Faculty of Applied Science and Engineering, University of Toronto, 10 King's College Road, Toronto, Ontario M5S 3G4, Canada.
Edward H
EH
Sargent
‡Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto, 144 College Street, Toronto, Ontario M5S 3M2, Canada.|§Institute for Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street Toronto, Ontario M5S 3G9, Canada.|⊥Department of Biochemistry, Faculty of Medicine, University of Toronto, 1 King's College Circle, Toronto, Ontario M5S 1A8, Canada.
Shana O
SO
Kelley
D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D009360:Neoplastic Cells, Circulating
cancer cell detection; cancer cell isolation; electrochemistry; integrated chip; microfluidics