Expression profiling of microarray gene signatures in acute and chronic myeloid leukaemia in human bone marrow.
BACKGROUND
Classification of cancer subtypes by means of microarray signatures is becoming increasingly difficult to ignore as a potential to transform pathological diagnosis; nonetheless, measurement of Indicator genes in routine practice appears to be arduous. In a preceding published study, we utilized real-time PCR measurement of Indicator genes in acute lymphoid leukaemia (ALL) and acute myeloid leukaemia (AML) as a way of application of microarray gene signatures. More to the point, the specificity of such genes for this distinction was investigated by their measurement in cases afflicted with chronic myeloid leukaemia (CML) and with normal bone marrow (BM).


METHODS
Mononuclear cells were sorted into unselected (total), CD34+ve, and CD34-ve fractions, mRNA globally amplified by using PolyA PCR. Moreover, the level of expression of 17 Indicator genes was identified by using real-time PCR.


RESULTS
No statistically significant difference was observed in expression for any gene among CML cases. Cyclin D3 (p≤0.04) was exclusively upregulated in CML in the CD34+ fraction, notwithstanding upregulation of HkrT-1 (p≤0.02) and fumarylacetoacetate (p≤0.03) in AML. HOXA9 experienced a non-significant upregulation in AML; however, in combination with proteoglycan 1 distinguished between AML and normal samples in the CD34- fraction in unsupervised clustering. Unsupervised clustering distinguished among AML and the other diagnostic groups.


CONCLUSIONS
The evidence from the present study suggests that the genes discriminatory between ALL and AML are uninformative in the context of CML and normal BM, excepting for distinction with AML.
Connective Tissue Disease Research Center, Department of Medical Genetics, Tabriz University of Medical Sciences, Tabriz, Iran.
E
E
Sakhinia
Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
M A
MA
Estiar
Neurosciences Research Center, Imam Reza Hospital, Tabriz University of Medical Sciences, Tabriz, Iran.
S
S
Andalib
Department of Pediatrics, Children Hospital, Tabriz University of Medical Sciences, Tabriz, Iran.
A
A
Rezamand
Gene Signature; Microarray; Myeloid Leukaemia; PolyA PCR; RT-PCR