Priming with DNA Enhances Considerably the Immunogenicity of hCG β-LTB Vaccine.
OBJECTIVE
Necessity to elicit antibody response above the protective threshold titres by sexually active women immunized to prevent pregnancy.


METHODS
Recombinant hCGβ-LTB vaccine expressed as both DNA and protein. Balb C mice employed for testing immunogenicity.


RESULTS
Necessity to give three primary injections of the vaccine to elicit proper antibody response. Immunization twice with DNA form of the vaccine at fortnightly interval followed by the protein elicits a distinctly higher antibody response than proteinic vaccine alone. Antibodies generated are bio-effective against hCG.


CONCLUSIONS
Immunization with the DNA form of the recombinant hCGβ-LTB vaccine twice at fortnightly interval followed by the proteinic form of the vaccine induces distinctly higher antibody response.
The Talwar Research Foundation, New Delhi, India.|Jamia Hamdard, New Delhi, India.
Kripa N
KN
Nand
The Talwar Research Foundation, New Delhi, India.
Jagdish C
JC
Gupta
National Institute of Immunology, New Delhi, India.
Amulya K
AK
Panda
Jamia Hamdard, New Delhi, India.
Swatantra K
SK
Jain
The Talwar Research Foundation, New Delhi, India.
Gursaran P
GP
Talwar
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000917:Antibody Formation / Q000276:immunology; D001427:Bacterial Toxins / Q000235:genetics / Q000276:immunology*; D018997:Chorionic Gonadotropin, beta Subunit, Human / Q000235:genetics / Q000276:immunology*; D003269:Contraception, Immunologic / Q000379:methods*; D004247:DNA / Q000008:administration & dosage / Q000276:immunology*; D004768:Enterotoxins / Q000235:genetics / Q000276:immunology*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000276:immunology*; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D011247:Pregnancy; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology*; D014611:Vaccination; D022642:Vaccines, Contraceptive / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology*
D000906:Antibodies; D001427:Bacterial Toxins; D018997:Chorionic Gonadotropin, beta Subunit, Human; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D011993:Recombinant Fusion Proteins; D022642:Vaccines, Contraceptive; D014614:Vaccines, Synthetic; D004247:DNA; C027538:heat-labile enterotoxin, E coli
DNA Vaccine; Mycobacterium indicus pranii (MiP); human chorionic gonadotropin (hCG)