Anti-genotoxic effect of naringin against bleomycin-induced genomic damage in human lymphocytes in vitro.
Naringin is a flavonoid found in grapefruit and other citrus fruits that shows antioxidant activity. The aim of the present study was to determine the anti-genotoxic and protective effects of naringin on the chemotherapeutic/radiomimetic agent bleomycin (BLM) in human blood lymphocyte cultures in vitro using micronucleus test and chromosomal aberrations (CA) assay. We tested the three doses of naringin (1, 2, 3 µg/mL) and a single dose of BLM (20 µg/mL). BLM significantly increased the total CAs and micronucleus frequency at a concentration of 20 µg/mL. Naringin did not show any toxicity in doses of 1, 2, and 3 µg/mL. Combined treatments of BLM and naringin (2 and 3 µg/mL) significantly reduced micronucleus formation. Naringin dose-dependently decreased the total chromosome aberrations frequency induced by BLM. These results indicate that naringin could prevent BLM (20 µg/mL)-induced genotoxicity.
a Department of Biology , Faculty of Science and Arts, Cell Culture and Genetic Toxicology Laboratory, Uludag University Görükle , Bursa , Turkey.
Dilek
D
Yilmaz
a Department of Biology , Faculty of Science and Arts, Cell Culture and Genetic Toxicology Laboratory, Uludag University Görükle , Bursa , Turkey.
Ozgun
O
Teksoy
a Department of Biology , Faculty of Science and Arts, Cell Culture and Genetic Toxicology Laboratory, Uludag University Görükle , Bursa , Turkey.
Rahmi
R
Bilaloglu
a Department of Biology , Faculty of Science and Arts, Cell Culture and Genetic Toxicology Laboratory, Uludag University Görükle , Bursa , Turkey.
Nilufer
N
Çinkilic
D000293:Adolescent; D000328:Adult; D016587:Antimutagenic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D001761:Bleomycin / Q000633:toxicity*; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D004249:DNA Damage / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005260:Female; D044950:Flavanones / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000648:ultrastructure; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced / Q000187:drug effects*; D055815:Young Adult
D016587:Antimutagenic Agents; D044950:Flavanones; D001761:Bleomycin; C005274:naringin
Bleomycin; chemotherapeutic/radiomimetic agent; chromosome aberration assay; flavonoid; micronucleus; naringin