Immediate and Ongoing Detection of Prions in the Blood of Hamsters  and Deer following Oral, Nasal, or Blood Inoculations.
Infectious prions traverse epithelial barriers to gain access to the circulatory system, yet the temporal parameters of transepithelial transport and persistence in the blood over time remain unknown. We used whole-blood real-time quaking-induced conversion (wbRT-QuIC) to analyze whole blood collected from transmissible spongiform encephalopathy (TSE)-inoculated deer and hamsters throughout the incubation period for the presence of common prion protein-conversion competent amyloid (PrPCCCA). We observed PrPC-CCA in the blood of TSE-inoculated hosts throughout the disease course from minutes postexposure to terminal disease.
Alan M
AM
Elder
Davin M
DM
Henderson
Amy V
AV
Nalls
Edward A
EA
Hoover
Anthony E
AE
Kincaid
Jason C
JC
Bartz
Candace K
CK
Mathiason
D000281:Administration, Intranasal; D000284:Administration, Oral; D000818:Animals; D001774:Blood Chemical Analysis; D003670:Deer; D007275:Injections, Intravenous; D008297:Male; D008647:Mesocricetus; D011328:Prions / Q000008:administration & dosage* / Q000097:blood*; D013997:Time Factors
D011328:Prions