A novel glucose oxidase biosensor based on poly([2,2';5',2″]-terthiophene-3'-carbaldehyde) modified electrode.
In the study, the electrochemical behavior of glucose oxidase (GOx) immobilized on poly([2,2';5',2″]-terthiophene-3'-carbaldehyde) (poly(TTP)) modified glassy carbon electrode (GCE) was investigated. The biosensor (poly(TTP)/GOx/GCE) showed a pair of redox peaks in 0.1 M phosphate buffer (pH 7.4) solution in the absence of oxygen the co-substrate of GOx. In here, Poly(TTP)/GOx/GCE biosensor acts as the co-substrate instead of oxygen. Upon the addition of glucose, the reduction and oxidation peak currents increased until the active site of GOx was fully saturated with glucose. The apparent m was estimated 26.13 mM from Lineweaver-Burk graph. The biosensor displayed a good stability and bioactivity. The biosensor showed a high sensitivity (56.1 nA/mM), a linear range (from 0.5 to 20.15 mM), and a good reproducibility with 3.6% of relative standard deviation. In addition, the interference currents of glycin, ascorbic acid, histidine, uric acid, dopamine, arginine, and fructose on GOx biosensor were investigated. All that substances exhibited an interference current under 10%. It was not shown a marked difference between GOx biosensor and spectrophotometric measurement of glucose in serum examples. UV-visible spectroscopy and scanning electron microscopy (SEM) experiments of the biosensor were also performed.
Yuzuncu Yil University, Faculty of Science, Department of Chemistry, 65080, Van, Turke. Electronic address: mguler@yyu.edu.tr.
Muhammet
M
Guler
Yuzuncu Yil University, Faculty of Science, Department of Chemistry, 65080, Van, Turke.
Vedat
V
Turkoglu
Yuzuncu Yil University, Faculty of Science, Department of Chemistry, 65080, Van, Turke.
Arif
A
Kivrak
D000447:Aldehydes / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D001786:Blood Glucose / Q000032:analysis*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013876:Thiophenes / Q000737:chemistry
D000447:Aldehydes; D001786:Blood Glucose; D004800:Enzymes, Immobilized; D013876:Thiophenes; D005949:Glucose Oxidase
Biosensor; Conducting polymer; Glucose oxidase