Osteoblastogenesis of Mesenchymal Stem Cells in 3-D Culture Enhanced by Low-Intensity Pulsed Ultrasound through Soluble Receptor Activator of Nuclear Factor Kappa B Ligand.
This study was performed to investigate osteoblastogenesis of human mesenchymal stem cells (hMSCs) cultured in 3-D scaffolds stimulated with low-intensity pulsed ultrasound and to identify the underlying mechanism mediated by soluble receptor activator of nuclear factor kappa B ligand (sRANKL) secreted by hMSCs. The results indicate that the mRNA levels of core-binding factor subunit alpha subunit 1 (CBFA1), osterix (OSX), alkaline phosphatase (ALP), osteocalcin and osteoprotegerin (OPG) and sRANKL production of hMSCs stimulated by ultrasound were significantly increased compared with the levels without ultrasound stimulation. Attenuating the sRANKL activity of ultrasound-treated hMSCs significantly reduced the mRNA expression of CBFA1, OSX, ALP and OPG. Adding sRANKL in hMSC culture significantly increased the mRNA expression of CBFA1, OSX and OPG. Together, the results suggest that osteoblastogenesis of hMSCs enhanced by ultrasound stimulation is mediated by endogenous sRANKL.
Department of Orthopedics and Rehabilitation, University of Wisconsin-Madison, Madison, Wisconsin, USA; Department of Biomedical Engineering, Chung Yuan Christian University, Chung-Li City, Tao-Yuan County, Taiwan.
Chun-Yi
CY
Chiu
Department of Orthopedics and Rehabilitation, University of Wisconsin-Madison, Madison, Wisconsin, USA; Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Tsung-Lin
TL
Tsai
Department of Orthopedics and Rehabilitation, University of Wisconsin-Madison, Madison, Wisconsin, USA; Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Ray
R
Vanderby
Orthopedic Development, Kensey Nash Corporation, Exton, Pennsylvania, USA.
Gino
G
Bradica
Department of Biomedical Engineering, Chung Yuan Christian University, Chung-Li City, Tao-Yuan County, Taiwan.
Shyh-Liang
SL
Lou
Department of Orthopedics and Rehabilitation, University of Wisconsin-Madison, Madison, Wisconsin, USA; Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, Wisconsin, USA. Electronic address: li@ortho.wisc.edu.
Wan-Ju
WJ
Li
D002454:Cell Differentiation / Q000502:physiology / Q000528:radiation effects; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism* / Q000528:radiation effects; D008875:Middle Aged; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism* / Q000528:radiation effects; D010012:Osteogenesis / Q000502:physiology / Q000528:radiation effects; D053245:RANK Ligand / Q000378:metabolism*; D011829:Radiation Dosage; D000069453:Ultrasonic Waves
D053245:RANK Ligand
Low-intensity pulsed ultrasound; Mesenchymal stem cell; Osteoblastogenesis; Receptor activator of nuclear factor kappa B ligand