Low-Cost Disposable Cartridge for Performing a White Blood Cell Count and Partial Differential at the Point-of-Care.
Being able to perform a white blood cell (WBC) count and differential is a crucial laboratory test for basic diagnostic practices. In this paper, we demonstrate proof of concept results for a disposable cartridge that could be used to perform a WBC count and 3-part differential at the point-of-care. The cartridge is composed of a glass slide, a layer of transfer tape, and a glass cover slip and incorporates acridine orange for cell staining and sub-type differentiation; the stained blood is then imaged, and image analysis techniques return a WBC count and 3-part differential. The cartridge was tested on a laboratory microscope with 3 normal samples, and had promising results with 85.7% of images resulting in a WBC count with ±15% of the true value. Further, the 3-part differential concentrations determined using the disposable cartridge had strong correlations with the true concentrations (R2values of 0.9986, 0.9421, and 0.6942 for granulocytes, lymphocytes, and monocytes, respectively). Preliminary designs for a low-cost, portable microscope have been created and are currently being prototyped.
Bioengineering Department, Rice University, Houston, TX 77005 USA (phone: 713-348-3022; catherine.majors@rice.edu ).
Catherine E
CE
Majors
Bioengineering Department, Rice University, Houston, TX 77005 USA. ( michal.e.pawlowski@rice.edu ).
Michal E
ME
Pawlowski
Bioengineering Department, Rice University, Houston, TX 77005 USA. ( ttkaczyk@rice.edu ).
Tomasz
T
Tkaczyk
Bioengineering Department, Rice University, Houston, TX 77005 USA, ( rkortum@rice.edu ).
Rebecca R
RR
Richards-Kortum