Salinity induced oxidative stress enhanced biofuel production potential of microalgae Scenedesmus sp. CCNM 1077.
Microalgal biomass is considered as potential feedstock for biofuel production. Enhancement of biomass, lipid and carbohydrate contents in microalgae is important for the commercialization of microalgal biofuels. In the present study, salinity stress induced physiological and biochemical changes in microalgae Scenedesmus sp. CCNM 1077 were studied. During single stage cultivation, 33.13% lipid and 35.91% carbohydrate content was found in 400 mM NaCl grown culture. During two stage cultivation, salinity stress of 400 mM for 3 days resulted in 24.77% lipid (containing 74.87% neutral lipid) along with higher biomass compared to single stage, making it an efficient strategy to enhance biofuel production potential of Scenedesmus sp. CCNM 1077. Apart from biochemical content, stress biomarkers like hydrogen peroxide, lipid peroxidation, ascorbate peroxidase, proline and mineral contents were also studied to understand the role of reactive oxygen species (ROS) mediated lipid accumulation in microalgae Scenedesmus sp. CCNM 1077.
Discipline of Salt & Marine Chemicals, CSIR - Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Academy of Scientific & Innovative Research (AcSIR), CSIR - Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India.
Imran
I
Pancha
Discipline of Salt & Marine Chemicals, CSIR - Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Academy of Scientific & Innovative Research (AcSIR), CSIR - Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India.
Kaumeel
K
Chokshi
Discipline of Salt & Marine Chemicals, CSIR - Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Academy of Scientific & Innovative Research (AcSIR), CSIR - Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India.
Rahulkumar
R
Maurya
Academy of Scientific & Innovative Research (AcSIR), CSIR - Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Discipline of Wasteland Research, CSIR- Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India.
Khanjan
K
Trivedi
Discipline of Salt & Marine Chemicals, CSIR - Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India.
Shailesh Kumar
SK
Patidar
Academy of Scientific & Innovative Research (AcSIR), CSIR - Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Discipline of Wasteland Research, CSIR- Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India.
Arup
A
Ghosh
Discipline of Salt & Marine Chemicals, CSIR - Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Academy of Scientific & Innovative Research (AcSIR), CSIR - Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India. Electronic address: smishra@csmcri.org.
Sandhya
S
Mishra
D060387:Ascorbate Peroxidases / Q000378:metabolism; D056804:Biofuels / Q000382:microbiology*; D015415:Biomarkers / Q000378:metabolism; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D006861:Hydrogen Peroxide / Q000378:metabolism; D008055:Lipids / Q000032:analysis; D008315:Malondialdehyde / Q000378:metabolism; D058086:Microalgae / Q000187:drug effects / Q000378:metabolism*; D008903:Minerals / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010860:Pigments, Biological / Q000378:metabolism; D011392:Proline / Q000378:metabolism; D054712:Salinity; D044444:Scenedesmus / Q000187:drug effects / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology
D056804:Biofuels; D015415:Biomarkers; D008055:Lipids; D008903:Minerals; D010860:Pigments, Biological; D012965:Sodium Chloride; D008315:Malondialdehyde; D011392:Proline; D006861:Hydrogen Peroxide; D060387:Ascorbate Peroxidases
Carbohydrate; Lipid; Microalgae; Oxidative stress; Salinity