[Role of PI3K/AKT pathways in mitomycin-mediated apoptosis of WB-F344 cells].
OBJECTIVE
To investigate the role of p38MAPK and PI3K/AKT pathways in mitomycin (MMC)-induced apoptosis in the liver stem-like cell line WB-F344.


METHODS
WB-F344 cells were exposed to MMC and apoptosis was evaluated by flow cytometry and DNA fragmentation. Phospho-MAPK and phospho-PI3K/AKT were detected by western blotting.


RESULTS
MMC induced apoptosis in WB-F344 cells at 6h after addition of MMC; the maximum level of apoptosis was reached at 24h after MMC exposure. The apoptosis effects of MMC were concentration dependent and inhibited when the PI3K pathway was abolished by the specific inhibitor LY294002, but not inhibited when the p38MAPK pathway was abolished by inhibitor SB203508.


CONCLUSIONS
Apoptosis of WB-F344 cells can be induced by MMC.Although MMC can activate both the PI3K/AKT and p38MAPK pathways, the apoptosis effect of MMC occurs via a PI3K pathway and is not dependent on the p38MAPK pathway.
Department of Liver Disease and Infectious Disease, Beijing Chaoyang Hospital Jingxi Campus, Beijing 100043, China.
Peng
P
Yao
Dawei
D
Yang
Darong
D
Hu
D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D002460:Cell Line; D002867:Chromones; D005434:Flow Cytometry; D016685:Mitomycin; D009025:Morpholines; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D051381:Rats; D015398:Signal Transduction; D048051:p38 Mitogen-Activated Protein Kinases
D002867:Chromones; D009025:Morpholines; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one; D016685:Mitomycin; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D048051:p38 Mitogen-Activated Protein Kinases