Investigation of epothilone B-induced cell death mechanisms in human epithelial cancer cells -in consideration of combined treatment with ionizing radiation.
Epothilone B was shown to have promising chemo- and radiosensitizing effects on cells, but the mechanisms underlying cell death remain ambiguous. The aim of the study was to examine selected cell death pathways on the basis of FaDu and A549 cells. Western blot analyses were used for investigation of specific apoptotic markers. Immunofluorescence imaging and flow cytometry were utilized for examination of cell death mechanisms. DNA-staining was used for studying influence of epothilone B on micronucleus rate. We showed that epothilone B can initiate cell death via apoptosis and mitotic catastrophe, but induction of cell death was cell type specific.
1Department of Radiotherapy and Radiation Oncology, University Medical Centre Rostock , Rostock , Germany.
Tonja
T
Baumgart
Stephan
S
Kriesen
Oliver
O
Neels
Guido
G
Hildebrandt
Katrin
K
Manda
D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D034261:Epothilones / Q000494:pharmacology*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D050257:Tubulin Modulators / Q000494:pharmacology
D034261:Epothilones; D011838:Radiation-Sensitizing Agents; D050257:Tubulin Modulators; C093788:epothilone B
Apoptosis; Epothilone B; Irradiation; Micronucleus; Mitotic catastrophe