[RNA-Seq and genuine traditional Chinese medicine].
RNA-Sequencing (RNA-Seq) is a newly-developed method in transcriptome research, it can afford more accurate transcription information and be more quickly by using Next-generation Sequencing (NGS) technology. RNA-Seq has been widely used in various biological fields. Genuine traditional Chinese medicines (TCM), with good quality and therapeutic effect, were always praised highly and used by famous physicians. The geo-herbalism formation of TCM is based on the product of the gene expression at specific space and time. So it has been a research hotspot to analyze the mechanism of biosynthesis through RNA-Seq in the study on the secondary metabolism of medicinal plant. This article mainly illustrates the RNA-Seq and its advantages, it also discusses the potential application in genuine TCM, and it can provide useful information for other researchers.
Xiao-Yue
XY
Wang
Jing-Yuan
JY
Song
Cai-Xiang
CX
Xie
Jian-Ping
JP
Han
Shi-Lin
SL
Chen
D004365:Drugs, Chinese Herbal; D020869:Gene Expression Profiling; D059014:High-Throughput Nucleotide Sequencing; D008516:Medicine, Chinese Traditional; D010946:Plants, Medicinal / Q000235:genetics*; D012313:RNA; D017423:Sequence Analysis, RNA; D059467:Transcriptome
D004365:Drugs, Chinese Herbal; D012313:RNA