Proteomic identification of mitochondrial carbonylated proteins in two maturation stages of pepper fruits.
Pepper fruits in green and red maturation stages were selected to study the protein pattern modified by oxidation measuring carbonylated proteins in isolated mitochondria, together with the accumulation of superoxide radical and hydrogen peroxide in the fruits. MALDI-TOF/TOF analysis identified as carbonylated proteins in both green and red fruits, formate dehydrogenase, NAD-dependent isocitrate dehydrogenase, porin, and defensin, pointing to a common regulation by carbonylation of these proteins independently of the maturation stage. However, other proteins such as glycine dehydrogenase P subunit and phosphate transporter were identified as targets of carbonylation only in green fruits, whereas aconitase, ATPase β subunit, prohibitin, orfB protein, and cytochrome C oxidase, were identified only in red fruits. In general, the results suggest that carbonylation of mitochondrial proteins is a PTM that drives the complex ripening process, probably establishing the accumulation and functionality of some mitochondrial proteins in the nonclimacteric pepper fruit.
Department of Stress Biology and Plant Pathology, CEBAS-CSIC, Murcia, Spain.
Daymi
D
Camejo
Department of Stress Biology and Plant Pathology, CEBAS-CSIC, Murcia, Spain.
Ana
A
Jiménez
Department of Biochemistry, Cellular and Molecular Biology of Plants, EEZ-CSIC, Granada, Spain.
José M
JM
Palma
Department of Stress Biology and Plant Pathology, CEBAS-CSIC, Murcia, Spain.
Francisca
F
Sevilla
D015153:Blotting, Western; D002212:Capsicum / Q000254:growth & development / Q000378:metabolism*; D003116:Color; D005638:Fruit / Q000254:growth & development / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000032:analysis* / Q000378:metabolism; D010084:Oxidation-Reduction; D010858:Pigmentation; D010940:Plant Proteins / Q000032:analysis* / Q000378:metabolism; D050050:Protein Carbonylation; D011499:Protein Processing, Post-Translational; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013481:Superoxides / Q000378:metabolism; D053719:Tandem Mass Spectrometry
D024101:Mitochondrial Proteins; D010940:Plant Proteins; D020543:Proteome; D013481:Superoxides; D006861:Hydrogen Peroxide
Carbonylation; Maturation; Mitochondria; Oxidation; Pepper fruit