Dietary intake of ain-93 standard diet induces Fatty liver with altered hepatic fatty acid profile in Wistar rats.
There are several standard diets for animals used in scientific research, usually conceived by scientific institutions. The AIN-93 diet is widely used, but there are some reports of fatty liver in Wistar rats fed this diet.



We aimed to evaluate the hepatic repercussions of the AIN-93 diet intake in Wistar rats.



Forty newly-weaned 21-day-old male Wistar rats were fed either the AIN-93 diet or a commercial diet for either 1 month or 4 months. Weight gain, serum biochemistry, hepatic histology, and hepatic fatty acid profile were analyzed.



Hepatic steatosis was observed, especially in the group fed the AIN-93 diet. Serum blood glucose, absolute and relative liver weight and hepatic levels of oleic, palmitoleic, stearic, and palmitic fatty acids were related to the observed steatosis, while lipidogram and serum markers of liver function and injury were not.



AIN-93 diet induced acute hepatic steatosis in Wistar rats, which may compromise its use as a standard diet for experimental studies with rodents. The hepatic fatty acid profile was associated with steatosis, with possible implications for disease prognosis.
Faculdade de Nutrição/ Universidade Federal de Alagoas, Av. Lourival de Melo Mota, s/n, Tabuleiro do Martins, Maceió-AL, Brasil, CEP 57.072-970.. jcfsnut@hotmail.com.
Juliana
J
Farias Santos
Faculdade de Nutrição/ Universidade Federal de Alagoas, Av. Lourival de Melo Mota, s/n, Tabuleiro do Martins, Maceió-AL, Brasil, CEP 57.072-970.. jcfsnut@hotmail.com.
Monique
M
Suruagy Amaral
Faculdade de Nutrição/ Universidade Federal de Alagoas, Av. Lourival de Melo Mota, s/n, Tabuleiro do Martins, Maceió-AL, Brasil, CEP 57.072-970.. jcfsnut@hotmail.com.
Suzana
S
Lima Oliveira
Faculdade de Nutrição/ Universidade Federal de Alagoas, Av. Lourival de Melo Mota, s/n, Tabuleiro do Martins, Maceió-AL, Brasil, CEP 57.072-970.. jcfsnut@hotmail.com.
Júnia
J
Porto Barbosa
Faculdade de Nutrição/ Universidade Federal de Alagoas, Av. Lourival de Melo Mota, s/n, Tabuleiro do Martins, Maceió-AL, Brasil, CEP 57.072-970.. jcfsnut@hotmail.com.
Cyro
C
Rego Cabral
Faculdade de Nutrição/ Universidade Federal de Alagoas, Av. Lourival de Melo Mota, s/n, Tabuleiro do Martins, Maceió-AL, Brasil, CEP 57.072-970.. jcfsnut@hotmail.com.
Ingrid
I
Sofia Melo
Faculdade de Nutrição/ Universidade Federal de Alagoas, Av. Lourival de Melo Mota, s/n, Tabuleiro do Martins, Maceió-AL, Brasil, CEP 57.072-970.. jcfsnut@hotmail.com.
Nassib
N
Bezerra Bueno
Coordenadoria de Química/ Instituto Federal de Alagoas, Rua Mizael Domingues n° 75, Centro, Maceió-AL, Brasil, CEP 57.020-600.. jcfsnut@hotmail.com.
Johnatan
J
Duarte Freitas
Instituto de Química e Biotecnologia/ Universidade Federal de Alagoas. Av. Lourival de Melo Mota, s/n, Tabuleiro do Martins, Maceió-AL, Brasil, CEP 57.072-970.. jcfsnut@hotmail.com.
Antônio
A
Goulart Sant'ana
Faculdade de Nutrição/ Universidade Federal de Alagoas, Av. Lourival de Melo Mota, s/n, Tabuleiro do Martins, Maceió-AL, Brasil, CEP 57.072-970.. jcfsnut@hotmail.com.
Terezinha
T
Rocha Ataíde
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D004032:Diet; D005227:Fatty Acids / Q000378:metabolism*; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008297:Male; D065626:Non-alcoholic Fatty Liver Disease / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D015430:Weight Gain
D001786:Blood Glucose; D005227:Fatty Acids