'Flatten plus': a recent implementation in WSxM for biological research.
Scanning probe microscopy (SPM) is already a relevant tool in biological research at the nanoscale. We present 'Flatten plus', a recent and helpful implementation in the well-known WSxM free software package. 'Flatten plus' allows reducing low-frequency noise in SPM images in a semi-automated way preventing the appearance of typical artifacts associated with such filters.


METHODS
WSxM is a free software implemented in C++ supported on MS Windows, but it can also be run under Mac or Linux using emulators such as Wine or Parallels. WSxM can be downloaded from http://www.wsxmsolutions.com/.


BACKGROUND
ignacio.horcas@wsxmsolutions.com.
Laboratorio de Nuevas Microscopías, Departamento de Física de la Materia Condensada, Universidad Autónoma de Madrid, E-28049 Madrid, WSxM solutions, C Júpiter, 7, E-28042 Madrid.
Alejandro
A
Gimeno
Laboratorio de Nuevas Microscopías, Departamento de Física de la Materia Condensada, Universidad Autónoma de Madrid, E-28049 Madrid.
Pablo
P
Ares
WSxM solutions, C Júpiter, 7, E-28042 Madrid.
Ignacio
I
Horcas
Nanotec Electrónica S.L., Ronda de Poniente 12, E-28760 Tres Cantos, Madrid and.
Adriana
A
Gil
Laboratorio de Nuevas Microscopías, Departamento de Física de la Materia Condensada, Universidad Autónoma de Madrid, E-28049 Madrid.
José M
JM
Gómez-Rodríguez
Departamento de Física, Facultad de Química. Universidad de Murcia, E-30100 Murcia, Spain.
Jaime
J
Colchero
Laboratorio de Nuevas Microscopías, Departamento de Física de la Materia Condensada, Universidad Autónoma de Madrid, E-28049 Madrid.
Julio
J
Gómez-Herrero
D000465:Algorithms; D035843:Biomedical Research; D018797:Cell Cycle Proteins / Q000737:chemistry* / Q000648:ultrastructure; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry* / Q000648:ultrastructure; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure; D006801:Humans; D007089:Image Enhancement; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D020527:Microscopy, Scanning Probe / Q000379:methods*; D012984:Software; D000098550:Structural Maintenance of Chromosome Protein 1
D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D000098550:Structural Maintenance of Chromosome Protein 1; D004247:DNA