First report of the characterization of the pathophysiological mechanisms caused by the freshwater catfish Pimelodus maculatus (order: Siluriformes).
Injuries caused by aquatic animals in Brazil in most cases are provoked by marine and freshwater catfish. Pimelodus maculatus is a freshwater catfish very common in Brazilian basins that causes frequent accidents mainly amongst fishermen, and whose venom characteristics and pathological mechanisms of the venom are poorly known. In the present study for the first time, we have characterized the main pathophysiological mechanisms associated with the clinical manifestation (pain, local inflammation and edema) of the envenomations caused by P. maculatus crude venom. It was estimated that the crude venom of one P. maculatus stinger contains approximately 100 μg of protein, likely the quantity involved in the envenomation. P. maculatus crude venom induced marked nociceptive and edematogenic effects and caused vascular permeability alterations at doses from 30 to 100 μg/animal. Additionally, P. maculatus crude venom caused a decrease in the contraction force in in situ frog heart, did not cause hemorrhage or alterations in clotting times (prothrombin time and activated partial thromboplastin time), but induced significant changes in the levels of CK and its isoenzyme CK-MB in mice. In the present work, we present a correlation between the effects obtained experimentally and the main symptoms observed in the human accidents provoked by P. maculatus.
Departamento de Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, 70910-900, DF, Brazil.
Beatriz Elena
BE
Sarmiento
Departamento de Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, 70910-900, DF, Brazil; Instituto Butantan, São Paulo, SP, Brazil.
Marisa
M
Rangel
Departamento de Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, 70910-900, DF, Brazil.
Jacqueline Coimbra
JC
Gonçalves
Departamento de Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, 70910-900, DF, Brazil.
Lilibete
L
Pereira
Departamento de Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, 70910-900, DF, Brazil.
Solange
S
Rego
Departamento de Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, 70910-900, DF, Brazil.
Leandro Ambrósio
LA
Campos
Faculdade de Medicina de Botucatu, Universidade Estadual Paulista, São Paulo, SP, Brazil.
Vidal
V
Haddad
Departamento de Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, 70910-900, DF, Brazil.
Márcia Renata
MR
Mortari
Departamento de Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, 70910-900, DF, Brazil. Electronic address: efschwa@unb.br.
Elisabeth F
EF
Schwartz
D000818:Animals; D001733:Bites and Stings / Q000503:physiopathology*; D001938:Brazil; D002199:Capillary Permeability / Q000187:drug effects; D002397:Catfishes / Q000378:metabolism*; D003402:Creatine Kinase / Q000378:metabolism; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000209:etiology / Q000503:physiopathology*; D005260:Female; D005397:Fish Venoms / Q000737:chemistry / Q000633:toxicity*; D005618:Fresh Water; D006470:Hemorrhage / Q000209:etiology / Q000503:physiopathology; D007249:Inflammation / Q000209:etiology / Q000503:physiopathology*; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D051379:Mice; D009619:Nociceptors / Q000187:drug effects / Q000473:pathology; D010146:Pain / Q000209:etiology / Q000503:physiopathology*; D010314:Partial Thromboplastin Time; D011517:Prothrombin Time; D051381:Rats; D017208:Rats, Wistar
D005397:Fish Venoms; D007527:Isoenzymes; D003402:Creatine Kinase
Cardiotoxic activity; Catfish; Edematogenic activity; Inflammatory activity; Pimelodus maculatus; Venom