[The application and epidemiological research of xTAG GPP multiplex PCR in the diagnosis of infectious diarrhea].
OBJECTIVE
To investigate the application value of xTAG (®) gastrointestinal pathogen panel (xTAG9(®) GPP) multiplex PCR in the early diagnosis of infectious diarrhea, and understand the epidemiology of intestinal diarrhea pathogens.


METHODS
Five hundred and ninety two specimens were collected in outpatient of Tongren Hospital, Capital Medical University, from 1st Oct 2013 to 30th Sep 2014, comparing the xTAG(®) GPP multiplex PCR assay with the traditional methods (culture, rapid enzyme immunoassay chromatography, microscopic examination, Real-time PCR) and mading the statistical analysis.


RESULTS
The positive rate of 592 patients with diarrhea specimens was 47.8% (283/592), the proportion of male and female was 1: 1.02, the average age was 31years. The virus detection rate was 18.1%, Rotavirus A was the most common organism detected (8.8%), concentrated in winter, popular in children.Secondly,Norovirus GI/GII (8.4%),Adenovirus 40/41 was five cases. The positive rate of bacteria was 35.5%, Enterotoxigenic E.coli (8.4%, 50/592) was most frequently detected in summer, common in young adults. The other pathogens were Campylobacter 7.7%, Salmonella 7.0%, Clostridium difficile toxinA/B 3.5%, Shigella 3.3%,E.coli O157 3.3% and Shiga toxin-producing E.coli LT/ST 1.7%.None of Yersinia enterocolitica and Vibrio cholerae was detected. There were ten samples with parasitic (1.7%), five samples were positive for Cryptosporidium, three for Entamoeba histolytica and two for Giardia. All of them did not have obvious distribution followed by season and population. Totally 242(40.8%) infected specimens with single pathogen were detected. There were 41 (6.9%) co-infections samples, including two pathogens 36 cases (6.1%), three pathogens in 5 cases (0.8%).


CONCLUSIONS
xTAG(®) GPP multiplex PCR is simple, sensitive, specific and can be used as a quick way to diagnose the infectious diarrhea. Diarrhea pathogen has significant characteristics with the season and crowd.
Department of Laboratory Medicine, Capital Medical University, Beijing Tongren Hospital, Beijing 100710, China.
Weiming
W
Feng
Department of Laboratory Medicine, Capital Medical University, Beijing Tongren Hospital, Beijing 100710, China.
Xiuli
X
Gu
Department of Laboratory Medicine, Capital Medical University, Beijing Tongren Hospital, Beijing 100710, China.
Wenjun
W
Sui
Department of Laboratory Medicine, Capital Medical University, Beijing Tongren Hospital, Beijing 100710, China.
Mingxin
M
Zhang
Department of Laboratory Medicine, Capital Medical University, Beijing Tongren Hospital, Beijing 100710, China.
Binghuai
B
Lu
Department of Laboratory Medicine, Capital Medical University, Beijing Tongren Hospital, Beijing 100710, China.
Mei
M
Wang
Department of Laboratory Medicine, Capital Medical University, Beijing Tongren Hospital, Beijing 100710, China.
Yanfei
Y
Huang
Department of Laboratory Medicine, Capital Medical University, Beijing Tongren Hospital, Beijing 100710, China. Email: luxinxin@trhos.com.
Xinxin
X
Lu
D000328:Adult; D003967:Diarrhea; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D060885:Multiplex Polymerase Chain Reaction; D060888:Real-Time Polymerase Chain Reaction