A multilocus phylogenetic analysis reveals the monophyly of a recircumscribed papilionoid legume tribe Diocleae with well-supported generic relationships.
Deciphering the phylogenetic relationships within the species-rich Millettioid clade has persisted as one of the major challenges in the systematics and evolutionary history of papilionoid legumes (Leguminosae, Papilionoideae). Historically, the predominantly neotropical lianas of subtribe Diocleinae in the Millettioid legumes have been taxonomically tangled together with the largely heterogeneous tribe Phaseoleae. This work presents a comprehensive molecular phylogenetic analysis based on nuclear and chloroplast markers and includes all genera ever referred to Diocleae except for the monospecific Philippine Luzonia, resolving several key generic relationships within the Millettioid legumes. The first of two separate analyses includes 310 matK accessions and strongly supports the reestablishment of tribe Diocleae as a branch of the Millettioid clade. This work sheds greater light on the higher-level phylogeny of Diocleae and allows the recognition of three major lineages: the Canavalia, Dioclea, and Galactia clades. The second set of phylogenetic analyses utilized nuclear (ITS/5.8S and ETS) and plastid (matK and trnT-Y) DNA sequences to reveal (i) the monophyly of Canavalia and Cleobulia; (ii) the monophyly of Bionia with the exclusion of Bionia bella; (iii) the paraphyly of Dioclea with respect to Cleobulia, Cymbosema, and Macropsychanthus; (iv) the paraphyly of Cratylia with respect to the broadly polyphyletic Camptosema; and (v) the polyphyly of Galactia with species scattered widely across the tree.
Universidade Estadual de Feira de Santana, Departamento de Ciências Biológicas, Av. Transnordestina, s/n, Novo Horizonte, 44036-900 Feira de Santana, Bahia, Brazil. Electronic address: luciano.paganucci@gmail.com.
Luciano Paganucci
LP
de Queiroz
Universidade Estadual de Feira de Santana, Departamento de Ciências Biológicas, Av. Transnordestina, s/n, Novo Horizonte, 44036-900 Feira de Santana, Bahia, Brazil.
José Floriano B
JF
Pastore
Universidade Estadual de Feira de Santana, Departamento de Ciências Biológicas, Av. Transnordestina, s/n, Novo Horizonte, 44036-900 Feira de Santana, Bahia, Brazil; Universidade Federal da Bahia, Instituto de Biologia, Departamento de Botânica, Rua Barão de Jeremoabo, s/n, Ondina, 40170-115 Salvador, Bahia, Brazil.
Domingos
D
Cardoso
Universidade Estadual de Feira de Santana, Departamento de Ciências Biológicas, Av. Transnordestina, s/n, Novo Horizonte, 44036-900 Feira de Santana, Bahia, Brazil.
Cristiane
C
Snak
Universidade Estadual de Feira de Santana, Departamento de Ciências Biológicas, Av. Transnordestina, s/n, Novo Horizonte, 44036-900 Feira de Santana, Bahia, Brazil.
Ana Luísa
AL
de C Lima
Institut de Recherche en Biologie Végétale, Département de Sciences Biologiques, Université de Montréal, 4101 rue Sherbrooke est, Montréal, Québec H1X 2B2, Canada.
Edeline
E
Gagnon
Smithsonian Institution, 10th and Constitution Ave, Washington, DC 20013-7012, USA; Department of Biology, Graduate School of Science, Chiba University, 1-33 Yayoi, Inage, Chiba, Japan.
Mohammad
M
Vatanparast
Queensland Herbarium, Brisbane Botanic Gardens, Mt Coot-tha Road, Toowong, Qld 4066, Australia.
Ailsa E
AE
Holland
Smithsonian Institution, 10th and Constitution Ave, Washington, DC 20013-7012, USA.
Ashley N
AN
Egan
D001499:Bayes Theorem; D005075:Biological Evolution; D002467:Cell Nucleus / Q000235:genetics; D002736:Chloroplasts / Q000235:genetics; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics; D052598:Dioclea / Q000145:classification*; D035264:Flowers / Q000235:genetics; D006801:Humans; D010802:Phylogeny; D018087:Plastids / Q000235:genetics; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D018744:DNA, Plant
Diocleinae; Leguminosae; Papilionoideae; Phaseoleae; Phylogeny