[Expression of fatty acid synthase and adipocyte fatty acid-binding protein and the relationship with the clinicopathological characteristics in human infiltrating ductal breast cancer].
OBJECTIVE
To study the expression of fatty acid synthase (FASN) and adipocyte fatty acid-binding protein (A-FABP) in human infiltrating ductal breast cancer (IDC) tissues and hunman breast cancer cells and the relationship with the clinicopathogical characteristics. To further explore the relationship between FASN and A-FABP, and the relevance of the invasion in cancer cell.


METHODS
The expression of FASN and A-FABP was detected in 58 cases of human infiltrating ductal breast cancer and 12 cases of human normal breast tissues by immunohistochemistry technique, calculated positive expression percentage according to the number of positive cells percentage and the staining degree of positive sediment. The cell wound-healing assay was applied to detect the invasion of SKBR3 and MCF-7 cells. Western blot was used to detect the expression of FASN and A-FABP in MCF-7 and SKBR3 cells.


RESULTS
The positive rates of FASN and A-FABP were 8.3% (1/12) and 16.7% (1/6) respectively in 12 cases of normal breast tissues by immunohistochemistry. In 58 cases of IDC tissues, the positive rates of FASN and A-FABP were 72.4% (42/58) and 79.3% (46/58) respectively. The differences of the positive rates of FASN and A-FABP in normal breast and IDC tissues were statistically significant (P<0.01, P<O=0.05). The expression of FASN and A-FABP was up-regulated in the lymph node metastasis positive group and the larger size group (tumor diameter>2 cm) when compared with lymph node metastasis negative group or the diameter < or =2 cm group, the differences were statistically significant (P<0.01). In IDC group, the expression of FASN correlated with A-FABP (r=0.797, P<0.001), The migration rate of SKBR3 was significantly higher than MCF-7 cell at 12, 24 h (P<0.05), FASN expression in SKBR3 was higher than that in MCF-7.


CONCLUSIONS
FASN and A-FABP might associated with the lymph node metastasis and tumor size, and there was correlation between FASN and A-FABP in human IDC tissues. FASN may associated with the invasion and metastasis in breast cancer cells.
Lan
L
Zhou
Yu-hua
YH
Zhao
Xiao-dong
XD
Wang
Su-fang
SF
Jiang
Hua
H
Li
D017667:Adipocytes; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000378:metabolism*; D018270:Carcinoma, Ductal, Breast / Q000378:metabolism*; D045744:Cell Line, Tumor; D054890:Fatty Acid Synthase, Type I / Q000378:metabolism*; D050556:Fatty Acid-Binding Proteins / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis
C493410:FABP4 protein, human; D050556:Fatty Acid-Binding Proteins; C518729:FASN protein, human; D054890:Fatty Acid Synthase, Type I