Immunomodulatory activity of methanolic extract of Amorphophallus commutatus var. wayanadensis under normal and cyclophosphamide induced immunosuppressive conditions in mice models.
The present study investigates the immunomodulatory activity of methanolic extract of Amorphophallus commutatus var. wayanadensis (MEAC) under normal and cyclophosphamide induced immunosuppressive conditions in Swiss albino mice models. The splenocyte proliferation assay was performed to study in-vitro immunomodulatory activity of MEAC, where sheep RBC (SRBC) was used to induce immune responses in the experimental animals. The in-vivo immunomodulatory activity was evaluated by humoral antibody titer, quantification of plaque forming cells, qualitative hemolysis, delayed type hypersensitivity assay, phagocytic index and neutrophil adhesion assays. The chemoprotective effect of MEAC was determined against cyclophosphamide induced immunosuppression in mice models. MEAC exhibited significant mitogenic and co-mitogenic activity on Con-A, PHA and LPS stimulated splenocytes isolated from mouse spleen in a dose dependent manner. Furthermore, MEAC also elicited significant immunomodulatory activity with enhanced activation of humoral immune response along with a suppressive effect on cell mediated immune response. Hematological and histopathological analysis revealed the protective effect of MEAC against CP induced immunosuppression. The significant immunomodulatory activity of MEAC observed in the current study could be due to the fatty acids and phytosterols present in the extract.
School of Bio Sciences and Technology, VIT University, Vellore 632014, India.
Sreena
S
Raj
School of Bio Sciences and Technology, VIT University, Vellore 632014, India. Electronic address: gothandam@yahoo.com.
K M
KM
Gothandam
D031056:Amorphophallus / Q000737:chemistry*; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003520:Cyclophosphamide / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005227:Fatty Acids / Q000032:analysis / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry; D006968:Hypersensitivity, Delayed / Q000139:chemically induced / Q000188:drug therapy; D056724:Immunity, Humoral / Q000187:drug effects; D056747:Immunomodulation; D007166:Immunosuppressive Agents / Q000633:toxicity*; D000432:Methanol; D051379:Mice; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects; D064209:Phytochemicals / Q000494:pharmacology; D010840:Phytosterols / Q000032:analysis / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology / Q000187:drug effects
D005227:Fatty Acids; D007166:Immunosuppressive Agents; D064209:Phytochemicals; D010840:Phytosterols; D010936:Plant Extracts; D003520:Cyclophosphamide; D000432:Methanol
Amorphophallus commutatus var. wayanadensis; Fatty acids; GC-MS; Immunomodulatory activity; Immunosuppression; Phytosterols