Topical application of Scutellaria baicalensis suppresses 2,4-dinitrochlorobenzene-induced contact dermatitis.
Allergic contact dermatitis (ACD) is a prototypic T-cell-mediated cutaneous inflammatory response. In the present study we describe the anti-allergic effect of topically applied Scutellaria bacalensis aqueous extract (WSBE) in suppressing 2,4-dinitrochlorobenzene (DNCB)-induced ACD in BALB/c mice. Topically applied WSBE attenuated the epidermal thickness and mast cell infiltration into the skin in DNCB-induced contact dermatitis. Furthermore, WSBE suppressed DNCB-induced production of serum IgE as well as IL-4, IFN-γ, and TNF-α in the skin. Topical application of WSBE also ameliorated the significant decrease in dermal glutathione and superoxide dismutase levels. Moreover, present results demonstrated that the baicalin, bioactive compound of WSBE, was able to penetrate into the skin following topical application, which was confirmed by the HPLC analysis using rat model. Taken together, topical application of WSBE exerts beneficial effects in contact dermatitis model, suggesting that WSBE might be a candidate for the treatment of contact dermatitis.
a College of Veterinary Medicine, Chungnam National University , Daejeon 305-764 , Republic of Korea.
Tae-Won
TW
Kim
b Graduated School of the New Drug Development, Chungnam National University , Daejeon 305-764 , Republic of Korea.
Jae-Mook
JM
Choi
c Central Research Institutes, Hawon Pharmaceutical , Jangheung-gun 529-851 , Republic of Korea.
Myoung-Seok
MS
Kim
a College of Veterinary Medicine, Chungnam National University , Daejeon 305-764 , Republic of Korea.
Hwa-Young
HY
Son
d Huons Research Institutes, College of Pharmacy, Hanyang University , Ansan 426-791 , Republic of Korea.
Jong-Hwan
JH
Lim
D000279:Administration, Cutaneous; D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D003877:Dermatitis, Contact / Q000188:drug therapy*; D004137:Dinitrochlorobenzene; D005260:Female; D005419:Flavonoids / Q000494:pharmacology; D007073:Immunoglobulin E / Q000097:blood; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D031364:Scutellaria baicalensis / Q000737:chemistry*; D012867:Skin / Q000187:drug effects*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D018926:Anti-Allergic Agents; D004137:Dinitrochlorobenzene; D005419:Flavonoids; D010936:Plant Extracts; C499814:Scutellaria baicalensis extract; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4; C038044:baicalin; D007073:Immunoglobulin E; D007371:Interferon-gamma
2,4-dinitrochlorobenzene; Scutellaria baicalesis; contact dermatitis; mouse