Temporal changes of microarchitectural and mechanical parameters of cancellous bone in the osteoporotic rabbit.
This study was aimed at elucidating the temporal changes of microarchitectural and mechanical parameters of cancellous bone in the osteoporotic rabbit model induced by ovariectomy (OVX) combined with glucocorticoid (GC) administration. Osteoporotic (OP) group received bilateral OVX combined with injections of GC, while sham group only received sham operation. Cancellous bone quality in vertebrae and femoral condyles in each group was assessed by DXA, μCT, nanoindentation, and biomechanical tests at pre-OVX and 4, 6, and 8 weeks after injection. With regard to femoral condyles, nanoindentation test could detect significant decline in tissue modulus and hardness at 4 weeks. However, BMD and microarchitecture of femoral condylar cancellous bone changed significantly at 6 weeks. In vertebrae, BMD, microarchitecture, nanoindentation, and biomechanical tests changed significantly at 4 weeks. Our data demonstrated that temporal changes of microarchitectural and mechanical parameters of cancellous bone in the osteoporotic rabbit were significant. The temporal changes of cancellous bone in different anatomical sites might be different. The nanoindentation method could detect the changes of bone quality at an earlier stage at both femoral condyle and vertebra in the osteoporotic rabbit model than other methods (μCT, BMD).
Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, Changle West Road No. 15, Xi'an, Shaanxi 710032, China.
Xin-Xin
XX
Wen
Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, Changle West Road No. 15, Xi'an, Shaanxi 710032, China.
Chao
C
Xu
Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, Changle West Road No. 15, Xi'an, Shaanxi 710032, China.
Fa-Qi
FQ
Wang
Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, Changle West Road No. 15, Xi'an, Shaanxi 710032, China.
Ya-Fei
YF
Feng
Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, Changle West Road No. 15, Xi'an, Shaanxi 710032, China.
Xiong
X
Zhao
Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, Changle West Road No. 15, Xi'an, Shaanxi 710032, China.
Ya-Bo
YB
Yan
Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, Changle West Road No. 15, Xi'an, Shaanxi 710032, China.
Wei
W
Lei
D015502:Absorptiometry, Photon; D000818:Animals; D001696:Biomechanical Phenomena; D015519:Bone Density; D001842:Bone and Bones / Q000000981:diagnostic imaging / Q000473:pathology* / Q000503:physiopathology*; D021621:Imaging, Three-Dimensional; D008159:Lumbar Vertebrae / Q000000981:diagnostic imaging / Q000473:pathology / Q000503:physiopathology; D008297:Male; D010024:Osteoporosis / Q000000981:diagnostic imaging / Q000473:pathology* / Q000503:physiopathology*; D011817:Rabbits; D013997:Time Factors; D055114:X-Ray Microtomography