Molecular Cloning and Characterisation of Farnesyl Pyrophosphate Synthase from Tripterygium wilfordii.
Farnesylpyrophosphate synthase (FPS) catalyzes the biosynthesis of farnesyl pyrophosphate (FPP), which is an important precursor of sesquiterpenoids such as artemisinin and wilfordine. In the present study, we report the molecular cloning and characterization of two full-length cDNAs encoding FPSs from Tripterygium wilfordii (TwFPSs). TwFPSs maintained their capability to synthesise FPP in vitro when purified as recombinant proteins from E. coli. Consistent with the endogenous role of FPS in FPP biosynthesis, TwFPSs were highly expressed in T. wilfordii roots, and were up-regulated upon methyl jasmonate (MeJA) treatment. The global gene expression profiles suggested that the TwFPSs might play an important regulatory role interpenoid biosynthesis in T. wilfordii, laying the groundwork for the future study of the synthetic biology of natural terpene products.
Capital Medical University School of Traditional Chinese Medicine, Capital Medical University, Beijing, People's Republic of China.
Yu-Jun
YJ
Zhao
Capital Medical University School of Traditional Chinese Medicine, Capital Medical University, Beijing, People's Republic of China.
Xin
X
Chen
Capital Medical University School of Traditional Chinese Medicine, Capital Medical University, Beijing, People's Republic of China.
Meng
M
Zhang
Capital Medical University School of Traditional Chinese Medicine, Capital Medical University, Beijing, People's Republic of China.
Ping
P
Su
Capital Medical University School of Traditional Chinese Medicine, Capital Medical University, Beijing, People's Republic of China.
Yu-Jia
YJ
Liu
Capital Medical University School of Traditional Chinese Medicine, Capital Medical University, Beijing, People's Republic of China.
Yu-Ru
YR
Tong
Capital Medical University School of Traditional Chinese Medicine, Capital Medical University, Beijing, People's Republic of China.
Xiu-Juan
XJ
Wang
National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, People's Republic of China.
Lu-Qi
LQ
Huang
Capital Medical University School of Traditional Chinese Medicine, Capital Medical University, Beijing, People's Republic of China.
Wei
W
Gao
D000085:Acetates / Q000494:pharmacology; D000595:Amino Acid Sequence; D055162:Biocatalysis / Q000187:drug effects; D003001:Cloning, Molecular; D003517:Cyclopentanes / Q000494:pharmacology; D018076:DNA, Complementary / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D008401:Gas Chromatography-Mass Spectrometry; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D051228:Geranyltranstransferase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000187:drug effects / Q000235:genetics; D054883:Oxylipins / Q000494:pharmacology; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D029757:Tripterygium / Q000201:enzymology* / Q000235:genetics
D000085:Acetates; D003517:Cyclopentanes; D018076:DNA, Complementary; D054883:Oxylipins; D010940:Plant Proteins; D011994:Recombinant Proteins; C072239:methyl jasmonate; D051228:Geranyltranstransferase