The end of the message: multiple protein-RNA interactions define the mRNA polyadenylation site.
The key RNA sequence elements and protein factors necessary for 3' processing of polyadenylated mRNA precursors are well known. Recent studies, however, have significantly reshaped current models for the protein-RNA interactions involved in poly(A) site recognition, painting a picture more complex than previously envisioned and also providing new insights into regulation of this important step in gene expression. Here we review the recent advances in this area and provide a perspective for future studies.
Department of Microbiology and Molecular Genetics, School of Medicine, University of California at Irvine, Irvine, California 92697, USA; jlm2@columbia.edu yongshes@uci.edu.
Yongsheng
Y
Shi
Department of Biological Sciences, Columbia University, New York, New York 10027, USA jlm2@columbia.edu yongshes@uci.edu.
James L
JL
Manley
D020816:Amino Acid Motifs; D039223:Cleavage And Polyadenylation Specificity Factor / Q000378:metabolism; D017124:Conserved Sequence; D005786:Gene Expression Regulation; D026723:Polyadenylation; D011485:Protein Binding; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*
D039223:Cleavage And Polyadenylation Specificity Factor; D012322:RNA Precursors; D012333:RNA, Messenger
gene expression; mRNA processing; poly(A) site recognition