[Effects of polygala on the neurogenesis of manganese poisoned mice].
OBJECTIVE
To investigate the effects of polygala on leaning and memory and the expression of Microtubule associated protein on manganese poisoned mice.


METHODS
60 female Kunming mice were randomly and equally divided into 5 group. They are normal control group (CG), manganese poisoned group (MG), manganese poisoned with polygala high dose group (MHG), manganese poisoned with polygala middle dose group (MMG), manganese poisoned with polygala low dose group (MLG). The model of manganese poisoned mice was prepared of the way of intraperitoneal injection of manganese chloride (MnCl2 15 mg/kg), the spatial learning and memory ability was tested by Morris water maze, the Doublecortin (DCX) was tested by the way of immunofluorescent staining in the SVZ and SGZ.


RESULTS
In the navigation test, compared with MG, the escape latency of MHG, MMG and MLG were significantly decreased (P < 0.05), in space exploration experiments, MHG, MMG, MLG compared with MG, the number increased significantly across platforms (P < 0.05). compared with MG, the DCX expression of MHG, MMG and MLG were significantly increased (P < 0.05).


CONCLUSIONS
The leaning and memory ability of manganese poisoned mice can be improved by the polygala, and the mechanism may be related to promote the expression of DCX and neurogenesis in the brain.
Department of Human Anatomy, Guangxi Medical University, Nanning 530021, China.
Xuechao
X
Jing
Department of Human Anatomy, Guangxi Medical University, Nanning 530021, China.
Li
L
Qin
Department of Human Anatomy, Guangxi Medical University, Nanning 530021, China. E-mail: yangbngx@sina.com.
Boning
B
Yang
D000818:Animals; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D005260:Female; D020149:Manganese Poisoning / Q000188:drug therapy*; D018782:Maze Learning / Q000187:drug effects; D008568:Memory / Q000187:drug effects; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000187:drug effects*; D055495:Neurogenesis / Q000187:drug effects*; D009479:Neuropeptides / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D031801:Polygala / Q000737:chemistry*
C000714328:DCX protein, human; C000714847:Dcx protein, mouse; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D008869:Microtubule-Associated Proteins; D009479:Neuropeptides; D010936:Plant Extracts