Bioluminescent whole-cell reporter gene assays as screening tools in the identification of antimicrobial natural product extracts.
We describe novel tools, bioluminescent whole-cell reporter gene assays, for facilitating the use of natural products in antimicrobial drug discovery. As proof-of-concept, a plant extract library was screened and follow-up experiments were carried out. Primary results can be obtained in 2-4h with high sensitivity, leading to significant improvements of the process.
Centre for Drug Research, Division of Pharmaceutical Biosciences, University of Helsinki, Helsinki, Finland.
Susanna
S
Nybond
Department of Chemistry and Bioengineering, Tampere University of Technology, Tampere, Finland.
Matti
M
Karp
Centre for Drug Research, Division of Pharmaceutical Biosciences, University of Helsinki, Helsinki, Finland.
Teijo
T
Yrjönen
Centre for Drug Research, Division of Pharmaceutical Biosciences, University of Helsinki, Helsinki, Finland. Electronic address: paivi.tammela@helsinki.fi.
Päivi
P
Tammela
D000890:Anti-Infective Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D001688:Biological Products / Q000302:isolation & purification* / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D017930:Genes, Reporter; D008163:Luminescent Measurements; D010936:Plant Extracts / Q000737:chemistry; D012680:Sensitivity and Specificity; D013997:Time Factors
D000890:Anti-Infective Agents; D001688:Biological Products; D010936:Plant Extracts
Antibacterial; Bioluminescence; Cell-based assay; Escherichia coli; Natural products; Screening