Use of alcohol vinegar in the inhibition of Candida spp. and its effect on the physical properties of acrylic resins.
BACKGROUND
Given the high prevalence of oral candidiasis and the restricted number of antifungal agents available to control infection, this study investigated the in vitro antifungal activity of alcohol vinegar on Candida spp. and its effect on the physical properties of acrylic resins.


METHODS
Tests to determine the Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC) of vinegar alcohol (0.04 g/ml of acetic acid) and nystatin (control) were performed. The antifungal activity of alcohol vinegar was assessed through microbial growth kinetic assays and inhibition of Candida albicans adhesion to acrylic resin at different intervals of time. Surface roughness and color of the acrylic resin were analyzed using a roughness meter and color analyzer device.


RESULTS
Alcohol vinegar showed MIC75% and MFC62.5% of 2.5 mg/ml, with fungicidal effect from 120 min, differing from nystatin (p < 0.0001), which showed fungistatic effect. Alcohol vinegar caused greater inhibition of C. albicans adhesion to the acrylic resin (p ≤ 0.001) compared to nystatin and did not change the roughness and color parameters of the material.


CONCLUSIONS
Alcohol vinegar showed antifungal properties against Candida strains and caused no physical changes to the acrylic resin.
Graduate Program in Dentistry, Universidade Federal da Paraíba, Campus I, João Pessoa, Paraíba, 58.051-900, Brazil. ricardodiasdecastro@yahoo.com.br.
Ricardo Dias
RD
de Castro
João Pessoa University Center, João Pessoa, Paraíba, Brazil. anacarolinaloureiro@hotmail.com.
Ana Carolina Loureiro Gama
AC
Mota
Graduate Program in Dentistry, Universidade Federal da Paraíba, Campus I, João Pessoa, Paraíba, 58.051-900, Brazil. edelolima@yahoo.com.br.
Edeltrudes
E
de Oliveira Lima
Graduate Program in Dentistry, Universidade Federal da Paraíba, Campus I, João Pessoa, Paraíba, 58.051-900, Brazil. andreulisses@yahoo.com.br.
André Ulisses Dantas
AU
Batista
Graduate Program in Dentistry, Universidade Federal da Paraíba, Campus I, João Pessoa, Paraíba, 58.051-900, Brazil. julyana86@hotmail.com.
Julyana
J
de Araújo Oliveira
Graduate Program in Dentistry, State University of Paraiba, Campina Grande, Paraíba, Brazil. dralessandro@ibest.com.br.
Alessandro Leite
AL
Cavalcanti
D019342:Acetic Acid / Q000737:chemistry / Q000494:pharmacology*; D000180:Acrylic Resins / Q000737:chemistry*; D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects; D002175:Candida / Q000187:drug effects*; D002176:Candida albicans / Q000187:drug effects; D041022:Candida tropicalis / Q000187:drug effects; D003116:Color; D003764:Dental Materials / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D009761:Nystatin / Q000494:pharmacology; D013053:Spectrophotometry / Q000379:methods; D013499:Surface Properties; D013997:Time Factors
D000180:Acrylic Resins; D000935:Antifungal Agents; D003764:Dental Materials; D009761:Nystatin; D019342:Acetic Acid