Tenuigenin ameliorates acute lung injury by inhibiting NF-κB and MAPK signalling pathways.
We aimed to explore the protective effect of tenuigenin (TNG) on lipopolysaccharide (LPS)-stimulated inflammatory responses in acute lung injury (ALI). Thus, we assessed the effects of TNG on the LPS-induced production of tumour necrosis factor (TNF)-α, interleukin (IL)-6, and IL-1β in the culture supernatants of RAW 264.7 cells. Male BALB/c mice were pretreated with commercial TNG (2, 4 and 8 mg/kg) and dexamethasone (Dex, 5mg/kg) for 1h prior to LPS (0.5 mg/kg) challenge. After 12h, airway inflammation was assessed. Our results showed that TNG dramatically decreased the production of TNF-α, IL-1β, and IL-6 in vitro and in vivo as well as the expression of COX-2 protein in vivo. Treatment with TNG not only significantly ameliorated LPS-stimulated histopathological changes but also reduced the myeloperoxidase (MPO) activity and the wet-to-dry weight ratio of the lungs. Furthermore, TNG blocked IκBα phosphorylation and degradation and inhibited p38/ERK phosphorylation in LPS-induced ALI. These findings suggest that TNG may have a protective effect on LPS-induced ALI and may be useful for the prevention and treatment of ALI in the clinical setting.
College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
Hongming
H
Lv
Department of Ophthalmology, The Second Hospital of Jilin University, 218 Ziqiang Street, Changchun 130041, Jilin Province, PR China.
Chao
C
Zhu
College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
Yuanjun
Y
Liao
College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
Yawen
Y
Gao
College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
Gejin
G
Lu
College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
Weiting
W
Zhong
College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
Yuwei
Y
Zheng
College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
Wei
W
Chen
College of Veterinary Medicine, Jilin University, Changchun 130062, PR China; Institutes of Translational Medicine, The First Hospital, Jilin University, Changchun 130001, PR China. Electronic address: cixinxin@gmail.com.
Xinxin
X
Ci
D055371:Acute Lung Injury / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002461:Cell Line, Transformed; D002470:Cell Survival / Q000187:drug effects; D051546:Cyclooxygenase 2 / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology / Q000627:therapeutic use; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D008070:Lipopolysaccharides / Q000633:toxicity; D008168:Lung / Q000187:drug effects / Q000473:pathology; D008264:Macrophages / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*; D013997:Time Factors
D000893:Anti-Inflammatory Agents; D016207:Cytokines; D004365:Drugs, Chinese Herbal; D008070:Lipopolysaccharides; D016328:NF-kappa B; C493220:tenuigenin; D003907:Dexamethasone; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; D020929:Mitogen-Activated Protein Kinase Kinases
Acute lung injury; Lipopolysaccharide; MAPKs; NF-κB; Tenuigenin