Chemistry-enabled methods for the visualization of cell-surface glycoproteins in Metazoans.
The majority of cell-surface and secreted proteins are glycosylated, which can directly affect their macromolecular interactions, stability, and localization. Investigating these effects is critical to developing a complete understanding of the role of glycoproteins in fundamental biology and human disease. The development of selective and unique chemical reactions have revolutionized the visualization, identification, and characterization of glycoproteins. Here, we review the chemical methods that have been created to enable the visualization of the major types of cell-surface glycoproteins in living systems, from mammalian cells to whole animals.
Department of Chemistry, University of Southern California, Los Angeles, CA, 90089-0744, USA.
Kelly N
KN
Chuh
Department of Chemistry, University of Southern California, Los Angeles, CA, 90089-0744, USA. matthew.pratt@usc.edu.|Department of Molecular and Computational Biology, University of Southern California, Los Angeles, CA, 90089-0744, USA. matthew.pratt@usc.edu.
Matthew R
MR
Pratt
D000818:Animals; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D005453:Fluorescence; D005643:Fucose / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D009077:Mucins / Q000737:chemistry / Q000378:metabolism*; D019158:N-Acetylneuraminic Acid / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics
D008562:Membrane Glycoproteins; D009077:Mucins; D005643:Fucose; D019158:N-Acetylneuraminic Acid
Cell-surface glycosylation; Chemical reporters; Fluorescent visualization