Effect of 5-aminolevulinic acid-encapsulate liposomes on photodynamic therapy in human cholangiocarcinoma cells.
Intracellular protoporphyrin IX (PpIX) generation following administration of 5-aminolevulinic acid (ALA) has been used in photodynamic therapy (PDT). Subsequent irradiation can lead to selective damage to photosensitizer-treated cells or tissues. In the present work, we describe the enhancement of ALA-induced PpIX accumulation using a liposome carrier. ALA-containing liposomes (Lipo-ALA) were prepared using dipalmitoyl-phosphatidyl choline and in vitro PDT effect was investigated against human cholangiocarcinoma HuCC-T1 cells. Lipo-ALA increased the uptake efficiency into tumor cells compared to ALA itself, which increased the phototoxic effect. A positive relationship was evident between small particle size, PpIX accumulation and cell death after Lipo-ALA based PDT.
Kyung Ha
KH
Choi
Chung-Wook
CW
Chung
Cy Hyun
CH
Kim
Do Hyung
DH
Kim
Young-Il
YI
Jeong
Dae Hwan
DH
Kang
D000622:Aminolevulinic Acid / Q000008:administration & dosage / Q000627:therapeutic use*; D017209:Apoptosis; D045744:Cell Line, Tumor; D002469:Cell Separation; D018281:Cholangiocarcinoma / Q000188:drug therapy* / Q000473:pathology; D005434:Flow Cytometry; D006801:Humans; D008081:Liposomes; D010778:Photochemotherapy
D008081:Liposomes; D000622:Aminolevulinic Acid