The influence of topical mitomycin-C on total fibroblasts, epithelialization, and collagenization in anoplasty wound healing in Wistar rats.
OBJECTIVE
The purpose of this study is to analyze the influence of topical mitomycin-C on total fibroblasts, epithelialization, and collagenization in anoplasty wound healing in Wistar rats.


METHODS
In this experimental study, anoplasty procedures were performed on twelve Wistar rats that were subsequently randomly placed into two groups; i.e., control and treated groups. In the treated group, topical mitomycin-C was applied to the wound following the anoplasty procedure. All rats were sacrificed on the seventh day, and tissue samples of the wounds were obtained for histopathological evaluations. The total fibroblasts, epithelialization, and collagenization were evaluated by scoring methods. The Mann-Whitney test was used to analyze the statistical significance of differences, and p values below 0.05 were considered to be significant.


RESULTS
The mean fibroblast scores for the treated and control groups were 1.5 and 3.0. The mean epithelialization scores for the treated and control groups were 5 and 3.33. The mean collagenization scores for the treated and control groups were 5 and 3.5. Statistical evaluations revealed significant differences between the groups in total fibroblasts (p=0.006), epithelialization (p=0.022), and collagenization (p=0.022).


CONCLUSIONS
Topical mitomycin-C reduced the fibroblasts; however, the treatment did not suppress epithelialization or collagenization during anoplasty wound healing in Wistar rats.
Division of Pediatric Surgery, Department of Surgery, Faculty of Medicine, Universitas Sam Ratulangi-Prof. R. D. Kandou General Hospital Manado, Jalan Raya Tanawangko No. 56, Manado 95115, Sulawesi Utara, Indonesia. Electronic address: drharsali@gmail.com.
Harsali Fransiscus
HF
Lampus
Department of Pediatric Surgery, Faculty of Medicine, Universitas Padjadjaran-Dr. Hasan Sadikin General Hospital Bandung, Jalan Pasteur no 38, Bandung 4016, Jawa Barat, Indonesia. Electronic address: dikkispb@yahoo.com.
Dikki Drajat
DD
Kusmayadi
Department of Pediatric Surgery, Faculty of Medicine, Universitas Padjadjaran-Dr. Hasan Sadikin General Hospital Bandung, Jalan Pasteur no 38, Bandung 4016, Jawa Barat, Indonesia. Electronic address: busarnawas@yahoo.com.
Bustanul Arifin
BA
Nawas
D000287:Administration, Topical; D001003:Anal Canal / Q000502:physiology / Q000601:surgery*; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage / Q000494:pharmacology*; D015415:Biomarkers / Q000378:metabolism; D003094:Collagen / Q000378:metabolism*; D005347:Fibroblasts / Q000187:drug effects* / Q000502:physiology; D016685:Mitomycin / Q000008:administration & dosage / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D062326:Re-Epithelialization / Q000187:drug effects / Q000502:physiology; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
D000903:Antibiotics, Antineoplastic; D015415:Biomarkers; D016685:Mitomycin; D003094:Collagen
Anoplasty; Collagenization; Epithelialization; Fibroblast; Topical mitomycin-C