Discovery and genetic analysis of non-bitter Tartary buckwheat (Fagopyrum tataricum Gaertn.) with trace-rutinosidase activity.
In a screening of about 500 lines of Tartary buckwheat, we identified lines that contained no detectable rutinosidase isozymes using an in-gel detection assay. We confirmed that seeds of these individuals had only a trace level of in-vitro rutinosidase activity. To investigate the heritability of the trace-rutinosidase characteristic, we analyzed the progeny of crosses between rutinosidase trace-lines, 'f3g-162', and the 'Hokkai T8'. The F2 progeny clearly divided into two groups: those with rutinosidase activity under 1.5 nkat/g seed (trace-rutinosidase) and those with activity over 400 nkat/g seed (normal rutinosidase). The segregation pattern of this trait in F2 progeny exhibited 1 : 3 ratio (trace-rutinosidase : normal rutinosidase), suggesting that the trace-rutinosidase trait is conferred by a single recessive gene; rutinosidase-trace A (rutA). In addition, sensory panelists evaluated the bitterness of flour from trace-rutinosidase individuals and did not detect bitterness, whereas flour from normal rutinosidase individuals was found to have strong bitterness. Although at least three bitter compounds have been reported in Tartary buckwheat seeds, our present findings indicate that rutin hydrolysis is the major contributing factor to bitterness. In addition, the trace-rutinosidase line identified here, 'f3g-162', is a promising material for generating a non-bitter Tartary buckwheat variety.
NARO Hokkaido Agricultural Research Center , Hitsujigaoka, Toyohira, Sapporo, Hokkaido 062-8555 , Japan.
Tatsuro
T
Suzuki
NARO Hokkaido Agricultural Research Center, Memuro Upland Farming Research Station , Shinsei, Memuro, Kasai, Hokkaido 082-0081 , Japan.
Toshikazu
T
Morishita
NARO Hokkaido Agricultural Research Center, Memuro Upland Farming Research Station , Shinsei, Memuro, Kasai, Hokkaido 082-0081 , Japan.
Yuji
Y
Mukasa
NARO Hokkaido Agricultural Research Center, Memuro Upland Farming Research Station , Shinsei, Memuro, Kasai, Hokkaido 082-0081 , Japan.
Shigenobu
S
Takigawa
NARO Hokkaido Agricultural Research Center, Memuro Upland Farming Research Station , Shinsei, Memuro, Kasai, Hokkaido 082-0081 , Japan.
Satoshi
S
Yokota
NARO Hokkaido Agricultural Research Center, Memuro Upland Farming Research Station , Shinsei, Memuro, Kasai, Hokkaido 082-0081 , Japan.
Koji
K
Ishiguro
NARO Hokkaido Agricultural Research Center, Memuro Upland Farming Research Station , Shinsei, Memuro, Kasai, Hokkaido 082-0081 , Japan.
Takahiro
T
Noda
Tartary buckwheat; bitterness; genetic resources; quality; rutinosidase