Probing cell-surface carbohydrate binding proteins with dual-modal glycan-conjugated nanoparticles.
Dual-modal fluorescent magnetic glyconanoparticles have been prepared and shown to be powerful in probing lectins displayed on pathogenic and mammalian cell surfaces. Blood group H1- and Le(b)-conjugated nanoparticles were found to bind to BabA displaying Helicobacter pylori, and Le(a)- and Le(b)-modified nanoparticles are both recognized by and internalized into DC-SIGN and SIGN-R1 expressing mammalian cells via lectin-mediated endocytosis. In addition, glyconanoparticles block adhesion of H. pylori to mammalian cells, suggesting that they can serve as inhibitors of infection of host cells by this pathogen. It has been also shown that owing to their magnetic properties, glyconanoparticles are useful tools to enrich lectin expressing cells. The combined results indicate that dual-modal glyconanoparticles are biocompatible and that they can be employed in lectin-associated biological studies and biomedical applications.
†Department of Chemistry, Yonsei University, Seoul 120-749 Korea.
Sungjin
S
Park
†Department of Chemistry, Yonsei University, Seoul 120-749 Korea.
Gun-Hee
GH
Kim
†Department of Chemistry, Yonsei University, Seoul 120-749 Korea.
Seong-Hyun
SH
Park
†Department of Chemistry, Yonsei University, Seoul 120-749 Korea.
Jaeyoung
J
Pai
†Department of Chemistry, Yonsei University, Seoul 120-749 Korea.
Dominea
D
Rathwell
‡Department of Veterinary Medicine, Department of Biomedical Science and Technology, Konkuk University, Seoul 143-701 Korea.
Jin-Yeon
JY
Park
‡Department of Veterinary Medicine, Department of Biomedical Science and Technology, Konkuk University, Seoul 143-701 Korea.
Young-Sun
YS
Kang
†Department of Chemistry, Yonsei University, Seoul 120-749 Korea.
Injae
I
Shin
D002236:Carbohydrate Conformation; D002241:Carbohydrates / Q000737:chemistry*; D005453:Fluorescence; D016480:Helicobacter pylori / Q000737:chemistry / Q000166:cytology; D006801:Humans; D037102:Lectins / Q000737:chemistry*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D014407:Tumor Cells, Cultured
D002241:Carbohydrates; D037102:Lectins; D058185:Magnetite Nanoparticles