Micropropagation of Dalbergia sissoo Roxb. through tissue culture technique.
Multiple shoots of Dalbergia sissoo Roxb. (Sissoo) were incited from seeds through indirect somatic embryogenesis method. Seeds were inoculated in Murashige and Skoog's medium without any growth hormone. Than cotyledonary leaves were struck and used for callus induction on MS medium amplified with 2, 4-dichlorophenoxyacetic acid (0.5 to 4 mg mL(-1)). After 3 to 4 weeks the embryogenic callus clumps was transferred to medium supplemented with cytokinin (BAP 1 to 5 mg L(-1), kinetin 1-5.0 mg L(-1)) for embryo maturation and germination. The high-frequency shoot proliferation (82%) and maximum number of shoots per explants were recorded in MS medium containing NAA (0.5)+BAP (0.5). The findings of recent investigations have shown that, it is possible to induce indirect somatic embryogenesis in Dalbergia sissoo and plant regeneration from callus cultures derived from cotyledonary leaves as explants.
Jyoti
J
Sahu
Shagufta
S
Khan
Ram Kumar
RK
Sahu
Amit
A
Roy
D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D049109:Cell Proliferation; D018548:Cotyledon / Q000254:growth & development; D003583:Cytokinins / Q000494:pharmacology; D035782:Dalbergia / Q000187:drug effects / Q000196:embryology / Q000254:growth & development*; D018525:Germination; D010937:Plant Growth Regulators / Q000494:pharmacology; D018520:Plant Shoots / Q000187:drug effects / Q000196:embryology / Q000254:growth & development*; D012038:Regeneration / Q000187:drug effects; D012639:Seeds / Q000187:drug effects / Q000196:embryology / Q000254:growth & development*; D046509:Tissue Culture Techniques
D003583:Cytokinins; D010937:Plant Growth Regulators; D015084:2,4-Dichlorophenoxyacetic Acid