Fluorescence reporting of G-quadruplex structures and modulating their DNAzyme activity using polyethylenimine-pyrene conjugate.
Four-stranded G-quadruplex structure is one of the most important non-canonical secondary structures of DNA formed by guanine (G)-rich sequences. G-rich DNA sequences are known to occur in the human genome, especially in the telomere 3' end and in oncogene promoters such as c-MYC and c-KIT. In this context, we designed pyrene-conjugated polyethylenimine (PEI-Py) as a fluorescence reporter for the recognition and detection of G-quadruplex structures of G-rich deoxyoligonucleotides and human telomere and gene promoter sequences, under ambient conditions. PEI-Py exhibited prominent pyrene excimer emission in the presence of G-quadruplex structures of G-rich deoxyoligonucleotides and biologically relevant DNA sequences. PEI-Py further displayed the modulation of DNAzyme activity of various G-quadruplex structures in the presence of hemin and hydrogen peroxide.
Bioorganic Chemistry Laboratory, New Chemistry Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur PO, Bengaluru 560064, India.
Nagarjun
N
Narayanaswamy
Bioorganic Chemistry Laboratory, New Chemistry Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur PO, Bengaluru 560064, India.
Manju
M
Unnikrishnan
Bioorganic Chemistry Laboratory, New Chemistry Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur PO, Bengaluru 560064, India.
Mona
M
Gupta
Bioorganic Chemistry Laboratory, New Chemistry Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur PO, Bengaluru 560064, India. Electronic address: tgraju@jncasr.ac.in.
T
T
Govindaraju
D021881:DNA, Catalytic / Q000037:antagonists & inhibitors*; D054856:G-Quadruplexes; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D061848:Optical Imaging; D011094:Polyethyleneimine / Q000737:chemistry / Q000494:pharmacology*; D011721:Pyrenes / Q000737:chemistry / Q000494:pharmacology*
D021881:DNA, Catalytic; D011721:Pyrenes; D011094:Polyethyleneimine
DNAzyme activity; Excimer emission; Fluorescence reporter; G-quadruplex; Polyethylenimine–pyrene conjugate