Human cytomegalovirus UL55, UL144, and US28 genotype distribution in infants infected congenitally or postnatally.
Cytomegalovirus (CMV) is the most common cause of congenital infection. This pathogen exhibits extensive genetic variability in the genes that encode structural envelope glycoproteins, regulatory proteins, and proteins that contribute to immune evasion. However, the role of specific viral strains in the outcome of congenital CMV infection is unclear. Variation in the UL55 gene encoding glycoprotein B (gB), the UL144 gene encoding TNF α-like receptor, and the US28 gene encoding β-chemokine receptor was determined in 60 newborn infants with congenital CMV infection and 90 infants with postnatal or undefined CMV infection. CMV polymorphisms were studied in relation to disease outcome and viral load. Genotyping was performed by a sequencing analysis of PCR-amplified fragments, and the viral load was measured by quantitative real-time PCR. The results demonstrated that (1) the UL55 and US28 genotype distributions were similar among the group of congenital and postnatal CMV infection; (2) the UL144 B1 genotype was more prevalent in congenital than in postnatal infection and was detected in 70% of newborns with asymptomatic congenital infection; and (3) none of the examined genotype was significantly linked with symptomatic CMV infection. No relationship was observed between genotype and viral load. The results revealed that UL55, UL144, and US28 polymorphisms are not associated with the outcome of CMV infection in infants, but the presence of UL144 B1 genotype might be virological marker of asymptomatic infection at birth.
Laboratory of Molecular Virology and Biological Chemistry, Institute of Medical Biology, Polish Academy of Sciences, Lodz, Poland.
Edyta
E
Paradowska
Laboratory of Molecular Virology and Biological Chemistry, Institute of Medical Biology, Polish Academy of Sciences, Lodz, Poland.
Mirosława
M
Studzińska
Laboratory of Molecular Virology and Biological Chemistry, Institute of Medical Biology, Polish Academy of Sciences, Lodz, Poland.
Patrycja
P
Suski
Department of Clinical Microbiology and Immunology, The Children's Memorial Health Institute, Warsaw, Poland.
Beata
B
Kasztelewicz
3rd Department of Pediatrics, Polish Mother's Memorial Hospital Research Institute, Lodz, Poland.
Małgorzata
M
Wiśniewska-Ligier
Department of Virology, Jagiellonian University Medical College, Cracow, Poland.
Barbara
B
Zawilińska
Department of Fetal-Maternal Medicine and Gynaecology, Polish Mother's Memorial Hospital Research Institute, Lodz, Poland.
Zuzanna
Z
Gaj
Department of Fetal-Maternal Medicine and Gynaecology, Polish Mother's Memorial Hospital Research Institute, Lodz, Poland.|Department of Fetal-Maternal Medicine and Gynaecology, 3rd Chair of Gynaecology and Obstetrics, Medical University, Lodz, Poland.
Dorota
D
Nowakowska
D000595:Amino Acid Sequence; D058345:Asymptomatic Infections / Q000453:epidemiology; D015415:Biomarkers / Q000097:blood / Q000134:cerebrospinal fluid / Q000737:chemistry / Q000652:urine; D003587:Cytomegalovirus / Q000235:genetics*; D003586:Cytomegalovirus Infections / Q000151:congenital* / Q000453:epidemiology / Q000821:virology*; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008562:Membrane Glycoproteins / Q000097:blood / Q000134:cerebrospinal fluid / Q000235:genetics* / Q000652:urine; D010802:Phylogeny; D011110:Polymorphism, Genetic; D060888:Real-Time Polymerase Chain Reaction; D019707:Receptors, Chemokine / Q000097:blood / Q000235:genetics*; D016415:Sequence Alignment; D017421:Sequence Analysis; D013997:Time Factors; D014759:Viral Envelope Proteins; D019562:Viral Load; D014764:Viral Proteins / Q000097:blood / Q000134:cerebrospinal fluid / Q000235:genetics* / Q000652:urine
D015415:Biomarkers; D008562:Membrane Glycoproteins; D019707:Receptors, Chemokine; C119624:UL144 ORF protein, Human herpesvirus 5; C546870:UL55 protein, human cytomegalovirus; C411191:US28 receptor, Cytomegalovirus; D014759:Viral Envelope Proteins; D014764:Viral Proteins
UL144; US28; congenital and postnatal infection; cytomegalovirus (CMV); gene polymorphism; glycoprotein B (gB)