Protein transduction domain of transactivating transcriptional activator fused to outer membrane protein K of Vibrio parahaemolyticus to vaccinate marbled eels (Anguilla marmorata) confers protection against mortality caused by V. parahaemolyticus.
Although immersion and oral vaccination are the most practical methods for fish farmers, their applications are very limited due to low immune stimulation effect. We used the protein transduction domain (PTD) of transactivating transcriptional factor (TAT) derived from HIV TAT protein to increase the delivery efficiency of aquatic protein vaccines. Vibrio parahaemolyticus outer membrane protein K (ompK), a reported vaccine candidate for the prevention of V. parahaemolyticus infection, was fused with TAT-PTD expressed in Escherichia coli. We found that PTD-ompK fusion protein effectively penetrated into marbled eel bodies. Analysis of ompK antibody titres demonstrated that immersion vaccination with PTD-ompK was superior to ompK alone and induced robust immune stimulation in marbled eels. Both active and passive protection analyses against immersive challenge with V. parahaemolyticus strains demonstrated that marbled eels immunized with PTD-ompK survived significantly longer than those immunized with ompK alone. Our results indicated that TAT-PTD could be served as is an efficient delivery system for aquatic immersion vaccinations against various infectious diseases commonly seen in aquatic farm industry.
Institute of Pharmaceutical Biotechnology and Engineering, College of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian, 350002, China.
Hang
H
Wang
Institute of Pharmaceutical Biotechnology and Engineering, College of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian, 350002, China.
Wei
W
Yang
Institute of Pharmaceutical Biotechnology and Engineering, College of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian, 350002, China.
Guoying
G
Shen
Institute of Pharmaceutical Biotechnology and Engineering, College of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian, 350002, China.
Jianting
J
Zhang
Institute of Pharmaceutical Biotechnology and Engineering, College of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian, 350002, China.
Wei
W
Lv
Institute of Pharmaceutical Biotechnology and Engineering, College of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian, 350002, China.
Binfeng
B
Ji
Institute of Pharmaceutical Biotechnology and Engineering, College of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian, 350002, China.
Chun
C
Meng
D000811:Anguilla / Q000276:immunology*; D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology*; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D016019:Survival Analysis; D015534:Trans-Activators / Q000235:genetics / Q000276:immunology*; D016896:Treatment Outcome; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014735:Vibrio Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D014736:Vibrio parahaemolyticus / Q000235:genetics / Q000276:immunology*; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000235:genetics / Q000276:immunology
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; D014614:Vaccines, Synthetic; D054322:tat Gene Products, Human Immunodeficiency Virus