Human epidermal growth factor receptor 2 expression in mixed gastric carcinoma.
OBJECTIVE
To investigate human epidermal growth factor receptor 2 (HER2) amplification and protein expression in mixed gastric carcinoma.


METHODS
Fluorescence in situ hybridization and immunohistochemistry were used to detect HER2 amplification and protein expression in 277 cases of mixed gastric carcinoma. Protein staining intensity was rate as 1+, 2+, or 3+.


RESULTS
Of the 277 cases, 114 (41.2%) expressed HER2 protein. HER2 3+ staining was observed in 28/277 (10.1%) cases, 2+ in 37/277 (13.4%) cases, and 1+ in 49/277 (17.7%) cases. A HER2 amplification rate of 17% was detected, of which 25/28 (89.3%) were observed in the HER2 3+ staining group, 17/37 (45.9%) in 2+, and 5/49 (10.2%) in 1+. Of the 47 patients with HER2 amplification who received chemotherapy plus trastuzumab, 22 demonstrated median progression-free and overall survivals of 9.1 mo and 16.7 mo, respectively, which were significantly better than those achieved with chemotherapy alone (5.6 mo and 12.1 mo, respectively) in 19 previously treated patients (Ps < 0.05).


CONCLUSIONS
HER2 detection in mixed gastric carcinoma displays high heterogeneity. Relatively quantitative parameters are needed for assessing the level of HER2 amplification and protein expression.
Yang-Kun Wang, Tian Yun, Xue-Xia Lv, Department of Pathology, 150 Luoyang Center Hospital, Luoyang 471031, Henan Province, China.
Yang-Kun
YK
Wang
Yang-Kun Wang, Tian Yun, Xue-Xia Lv, Department of Pathology, 150 Luoyang Center Hospital, Luoyang 471031, Henan Province, China.
Zhong
Z
Chen
Yang-Kun Wang, Tian Yun, Xue-Xia Lv, Department of Pathology, 150 Luoyang Center Hospital, Luoyang 471031, Henan Province, China.
Tian
T
Yun
Yang-Kun Wang, Tian Yun, Xue-Xia Lv, Department of Pathology, 150 Luoyang Center Hospital, Luoyang 471031, Henan Province, China.
Cong-Yang
CY
Li
Yang-Kun Wang, Tian Yun, Xue-Xia Lv, Department of Pathology, 150 Luoyang Center Hospital, Luoyang 471031, Henan Province, China.
Bo
B
Jiang
Yang-Kun Wang, Tian Yun, Xue-Xia Lv, Department of Pathology, 150 Luoyang Center Hospital, Luoyang 471031, Henan Province, China.
Xue-Xia
XX
Lv
Yang-Kun Wang, Tian Yun, Xue-Xia Lv, Department of Pathology, 150 Luoyang Center Hospital, Luoyang 471031, Henan Province, China.
Guang-Hui
GH
Chu
Yang-Kun Wang, Tian Yun, Xue-Xia Lv, Department of Pathology, 150 Luoyang Center Hospital, Luoyang 471031, Henan Province, China.
Su-Nan
SN
Wang
Yang-Kun Wang, Tian Yun, Xue-Xia Lv, Department of Pathology, 150 Luoyang Center Hospital, Luoyang 471031, Henan Province, China.
Hui
H
Yan
Yang-Kun Wang, Tian Yun, Xue-Xia Lv, Department of Pathology, 150 Luoyang Center Hospital, Luoyang 471031, Henan Province, China.
Lei-Feng
LF
Shi
D000328:Adult; D000368:Aged; D000970:Antineoplastic Agents / Q000627:therapeutic use; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics; D002277:Carcinoma / Q000737:chemistry* / Q000188:drug therapy / Q000235:genetics / Q000401:mortality / Q000473:pathology; D018450:Disease Progression; D018572:Disease-Free Survival; D005260:Female; D005784:Gene Amplification; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D018193:Neoplasms, Complex and Mixed / Q000737:chemistry* / Q000188:drug therapy / Q000235:genetics / Q000401:mortality / Q000473:pathology; D018579:Patient Selection; D011237:Predictive Value of Tests; D012333:RNA, Messenger / Q000032:analysis; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000235:genetics; D013274:Stomach Neoplasms / Q000737:chemistry* / Q000188:drug therapy / Q000235:genetics / Q000401:mortality / Q000473:pathology; D013997:Time Factors; D000068878:Trastuzumab / Q000627:therapeutic use; D016896:Treatment Outcome
D000970:Antineoplastic Agents; D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D018719:Erb-b2 Receptor Tyrosine Kinases; D000068878:Trastuzumab; C508053:ERBB2 protein, human
Fluorescence in situ hybridization; Gastric pathology; Human epidermal growth factor receptor 2; Immunohistochemistry; Stomach