Loss of Faap20 Causes Hematopoietic Stem and Progenitor Cell Depletion in Mice Under Genotoxic Stress.
20-kDa FANCA-associated protein (FAAP20) is a recently identified protein that associates with the Fanconi anemia (FA) core complex component, FANCA. FAAP20 contains a conserved ubiquitin-binding zinc-finger domain and plays critical roles in the FA-BRCA pathway of DNA repair and genome maintenance. The function of FAAP20 in animals has not been explored. Here, we report that deletion of Faap20 in mice led to a mild FA-like phenotype with defects in the reproductive and hematopoietic systems. Specifically, hematopoietic stem and progenitor cells (HSPCs) from Faap20(-) (/) (-) mice showed defects in long-term multilineage reconstitution in lethally irradiated recipient mice, with milder phenotype as compared to HSPCs from Fanca(-) (/) (-) or Fancc(-) (/) (-) mice. Faap20(-) (/) (-) mice are susceptible to mitomycin C (MMC)-induced pancytopenia. That is, acute MMC stress induced a significant progenitor loss especially the erythroid progenitors and megakaryocyte-erythrocyte progenitors in Faap20(-) (/) (-) mice. Furthermore, Faap20(-) (/) (-) HSPCs displayed aberrant cell cycle pattern during chronic MMC treatment. Finally, using Faap20(-) (/) (-) Fanca(-) (/) (-) double-knockout mice, we demonstrated a possible dominant effect of FANCA in the interaction between FAAP20 and FANCA. This novel Faap20 mouse model may be valuable in studying the regulation of the FA pathway during bone marrow failure progress in FA patients.
Division of Experimental Hematology and Cancer Biology.
Tingting
T
Zhang
Division of Experimental Hematology and Cancer Biology.
Andrew F
AF
Wilson
Division of Experimental Hematology and Cancer Biology.
Abdullah
A
Mahmood Ali
Division of Reproductive Sciences, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA.
Satoshi H
SH
Namekawa
Division of Experimental Hematology and Cancer Biology.
Paul R
PR
Andreassen
Division of Experimental Hematology and Cancer Biology.
Amom
A
Ruhikanta Meetei
Division of Experimental Hematology and Cancer Biology.
Qishen
Q
Pang
D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation; D004249:DNA Damage; D005199:Fanconi Anemia / Q000235:genetics* / Q000378:metabolism* / Q000401:mortality; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D013234:Stem Cells; D016019:Survival Analysis
D000604:Amino Acyl-tRNA Synthetases; C556158:FAAP protein, mouse
Cell cycle; Erythroid differentiation; Fanconi anemia; Hematopoietic progenitors