Comparison of bacterial community structures of terrestrial cyanobacterium Nostoc flagelliforme in three different regions of China using PCR-DGGE analysis.
Filamentous Nostoc flagelliforme form colloidal complex, with beaded cells interacting with other bacteria embedded in the complex multilayer sheath. However, the species of bacteria in the sheath and the interaction between N. flagelliforme and associated bacteria remain unclear. In this study, PCR-denaturing gradient gel electrophoresis (DGGE) was used to investigate the bacterial communities of N. flagelliforme from three regions of China. DGGE patterns showed variations in all samples, exhibiting 25 discrete bands with various intensities. The diversity index analysis of bands profiles suggested the high similarity of bacterial communities to each other but also the dependence of microbial composition on each location. Phylogenetic affiliation indicated that the majority of the sequences obtained were affiliated with Actinobacteria, Cyanobacteria, Proteobacteria, Acidobacteria, Bacteroidetes, of which Cyanobacteria was dominant, followed the Proteobacteria. Members of the genus Nostoc were the most abundant in all samples. Rhizobiales and Actinobacteria were identified, whereas, Craurococcus, Caulobacter, Pseudomonas, Terriglobus and Mucilaginibacter were also identified at low levels. Through comparing the bacterial composition of N. flagelliforme from different regions, it was revealed that N. flagelliforme could facilitate the growth of other microorganisms including both autotrophic bacteria and heterotrophic ones and positively contributed to their harsh ecosystems. The results indicated N. flagelliforme played an important role in diversifying the microbial community composition and had potential application in soil desertification.
Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, School of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, People's Republic of China.
Pei-pei
PP
Han
Shi-gang
SG
Shen
Shi-ru
SR
Jia
Hui-yan
HY
Wang
Cheng
C
Zhong
Zhi-lei
ZL
Tan
He-xin
HX
Lv
D001419:Bacteria / Q000145:classification* / Q000235:genetics; D002681:China; D004269:DNA, Bacterial / Q000032:analysis*; D058645:Denaturing Gradient Gel Electrophoresis; D017753:Ecosystem; D046937:Nostoc / Q000502:physiology*; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D004269:DNA, Bacterial