Gastroprotective potential of Buddleja scordioides Kunth Scrophulariaceae infusions; effects into the modulation of antioxidant enzymes and inflammation markers in an in vivo model.
BACKGROUND
A common plant used to treat several gastric disorders is Buddleja scordioides Kunth, commonly known as salvilla.


OBJECTIVE
To detect inflammatory markers, in order to evaluate the gastroprotective potential of salvilla infusions, as this could have beneficial impact on the population exposed to gastric ulcers and colitis.


METHODS
The present work attempted infusions were prepared with B. scordioides (1% w/w) lyophilized and stored. Total phenolic content and GC-MS analysis were performed. Wistar rats were divided into five groups (n=8), a negative vehicle control, an indomethacin group, and three experimental groups, named preventive, curative, and suppressive. All rats were sacrificed under deep ether anesthesia (6h) after the last oral administration of indomethacin/infusion. The rat stomachs were promptly excised, weighed, and chilled in ice-cold and 0.9% NaCl. Histological analysis, nitrites quantification and immunodetection assays were done.


RESULTS
B. scordioides infusions markedly reduced the visible hemorrhagic lesions induced by indomethacin in rat stomachs, also showed down-regulation of COX2, IL-8 and TNFα and up-regulation of COX-1 with a moderate down-regulation of NFkB and lower amount of nitrites. However, this behavior was dependent on the treatment, showing most down-regulation of COX-2, TNFα and IL-8 in the curative treatment; more down-regulation of NF-kB in the preventive treatment; and more up-regulation of COX-1 for the suppressor and preventive treatments.


CONCLUSIONS
The anti-inflammatory potential of B. scordioides infusions could be related with the presence of polyphenols as quercetin in the infusion and how this one is consumed.
Instituto Tecnológico de Durango, Blvd. Felipe Pescador 1830 Ote., Col. Nueva Vizcaya, C.P. 34080 Durango, Durango, México.
J O
JO
Díaz-Rivas
Instituto Tecnológico de Durango, Blvd. Felipe Pescador 1830 Ote., Col. Nueva Vizcaya, C.P. 34080 Durango, Durango, México.
E
E
Herrera-Carrera
Instituto Tecnológico de Durango, Blvd. Felipe Pescador 1830 Ote., Col. Nueva Vizcaya, C.P. 34080 Durango, Durango, México. Electronic address: agallegos@itdurango.edu.mx.
J A
JA
Gallegos-Infante
Instituto Tecnológico de Durango, Blvd. Felipe Pescador 1830 Ote., Col. Nueva Vizcaya, C.P. 34080 Durango, Durango, México. Electronic address: nrocha@itdurango.edu.mx.
N E
NE
Rocha-Guzmán
Instituto Tecnológico de Durango, Blvd. Felipe Pescador 1830 Ote., Col. Nueva Vizcaya, C.P. 34080 Durango, Durango, México.
R F
RF
González-Laredo
Instituto Tecnológico de Durango, Blvd. Felipe Pescador 1830 Ote., Col. Nueva Vizcaya, C.P. 34080 Durango, Durango, México.
M R
MR
Moreno-Jiménez
Universidad Autónoma de Querétaro, Querétaro, Facultad de Química., C.U., Cerro de las Campanas, C.P. 76010 Querétaro, Querétaro, México.
M
M
Ramos-Gómez
Universidad Autónoma de Querétaro, Querétaro, Facultad de Química., C.U., Cerro de las Campanas, C.P. 76010 Querétaro, Querétaro, México.
R
R
Reynoso-Camacho
Universidad Europea de Madrid, Calle Tajo, s/n, Villaviciosa de Odón, 28670 Madrid, España.
M
M
Larrosa-Pérez
Laboratorio de Histopatología, Facultad de Medicina, Universidad Autónoma de Querétaro, Clavel 200, Col. Prados de la Capilla, Querétaro 76017, México.
M A
MA
Gallegos-Corona
D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology; D000975:Antioxidants / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D032861:Buddleja / Q000737:chemistry*; D015536:Down-Regulation / Q000187:drug effects; D005753:Gastric Mucosa / Q000378:metabolism; D007213:Indomethacin / Q000009:adverse effects*; D018836:Inflammation Mediators / Q000378:metabolism*; D008297:Male; D009573:Nitrites / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D013270:Stomach / Q000187:drug effects / Q000473:pathology; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000517:prevention & control*; D015854:Up-Regulation / Q000187:drug effects
D000897:Anti-Ulcer Agents; D000975:Antioxidants; D015415:Biomarkers; D018836:Inflammation Mediators; D009573:Nitrites; D010936:Plant Extracts; D007213:Indomethacin
Buddleja scordioides; Gastric damage; Indomethacin; Inflammation; NSAIDs