Tissue-engineered acellular small diameter long-bypass grafts with neointima-inducing activity.
Researchers have attempted to develop efficient antithrombogenic surfaces, and yet small-caliber artificial vascular grafts are still unavailable. Here, we demonstrate the excellent patency of tissue-engineered small-caliber long-bypass grafts measuring 20-30 cm in length and having a 2-mm inner diameter. The inner surface of an acellular ostrich carotid artery was modified with a novel heterobifunctional peptide composed of a collagen-binding region and the integrin α4β1 ligand, REDV. Six grafts were transplanted in the femoral-femoral artery crossover bypass method. Animals were observed for 20 days and received no anticoagulant medication. No thrombogenesis was observed on the luminal surface and five cases were patent. In contrast, all unmodified grafts became occluded, and severe thrombosis was observed. The vascular grafts reported here are the first successful demonstrations of short-term patency at clinically applicable sizes.
Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, Fujishiro-dai, Suita, Osaka 565-8565, Japan.
Atsushi
A
Mahara
Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, Fujishiro-dai, Suita, Osaka 565-8565, Japan; Department of Biobased Materials Science, Kyoto Institute of Technology, Sakyo-ku, Kyoto 606-8585, Japan.
Shota
S
Somekawa
Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, Fujishiro-dai, Suita, Osaka 565-8565, Japan; Faculty of Chemistry, Materials and Bioengineering, Kansai University, 3-3-35 Yamatecho, Suita, Osaka 565-8680, Japan.
Naoki
N
Kobayashi
Faculty of Chemistry, Materials and Bioengineering, Kansai University, 3-3-35 Yamatecho, Suita, Osaka 565-8680, Japan.
Yoshiaki
Y
Hirano
Department of Biobased Materials Science, Kyoto Institute of Technology, Sakyo-ku, Kyoto 606-8585, Japan.
Yoshiharu
Y
Kimura
Department of Biomedical Engineering, Osaka Institute of Technology, 5-16-1, Omiya, Asahi-ku, Osaka 535-8585, Japan.
Toshiya
T
Fujisato
Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, Fujishiro-dai, Suita, Osaka 565-8565, Japan. Electronic address: yamtet@ncvc.go.jp.
Tetsuji
T
Yamaoka
D000818:Animals; D000925:Anticoagulants / Q000737:chemistry; D001705:Bioprosthesis; D001807:Blood Vessel Prosthesis; D001808:Blood Vessels / Q000473:pathology; D002339:Carotid Arteries / Q000378:metabolism*; D005263:Femoral Artery / Q000601:surgery; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D039041:Integrin alpha4beta1 / Q000737:chemistry; D008024:Ligands; D058426:Neointima / Q000473:pathology*; D010455:Peptides / Q000737:chemistry; D020310:Struthioniformes; D013927:Thrombosis / Q000517:prevention & control*; D023822:Tissue Engineering / Q000379:methods*; D014654:Vascular Patency
D000925:Anticoagulants; D039041:Integrin alpha4beta1; D008024:Ligands; D010455:Peptides
Acellular; Long bypass; Neointima; Small-diameter vascular graft; Tissue engineering