SIRT7 Exhibits Oncogenic Potential in Human Ovarian Cancer Cells.
BACKGROUND
Sirtuin7 (SIRT7) is a type of nicotinamide adenine dinucleotide oxidized form (NAD+)-dependent deacetylase and the least understood member of the sirtuins family; it is implicated in various processes, such as aging, DNA damage repair and cell signaling transduction. There is some evidence that SIRT7 may function as a tumor trigger for human malignancy. Here, we aimed to explore the biological function of SIRT7 in ovarian carcinoma cells and its potential mechanism.


METHODS
Expression of SIRT7 in ovarian cancer cell lines was detected by western blotting. Transduced cell lines with SIRT7 knockdown or overexpression were constructed. Cell viability, cologenic, apoptosis-associated and motility assays were performed to elucidate the biological function of SIRT7 in ovarian cancer cells.


RESULTS
SIRT7 demonstrated a higher level in ovarian cancer cell lines compared with normal cells. On the one hand, down-regulation of SIRT7 significantly reduced ovarian cancer cell growth, repressed colony formation and increased cancer cell apoptosis; on the other hand, up-regulation promoted the migration of cancer cells. Additionally, repression of SIRT7 also induced change in apoptosis-related molecules and subunits of the NF-κB family.


CONCLUSIONS
In the present study, our data indicated that SIRT7 might play a role of oncogene in ovarian malignancy and be a potential therapeutic target.
Department of Clinical Laboratory, Fudan University Shanghai Cancer Center, China E-mail : guolin500@hotmail.com.
Hong-Ling
HL
Wang
Ren-Quan
RQ
Lu
Su-Hong
SH
Xie
Hui
H
Zheng
Xue-Mei
XM
Wen
Xiang
X
Gao
Lin
L
Guo
D017209:Apoptosis / Q000235:genetics*; D002277:Carcinoma / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics; D002470:Cell Survival / Q000235:genetics; D015536:Down-Regulation; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism; D009857:Oncogenes; D010051:Ovarian Neoplasms / Q000235:genetics*; D010053:Ovary / Q000378:metabolism*; D037761:Sirtuins / Q000235:genetics*; D015854:Up-Regulation
D016328:NF-kappa B; C510661:SIRT7 protein, human; D037761:Sirtuins