A comparative calorimetric and spectroscopic study of the effects of cholesterol and of the plant sterols β-sitosterol and stigmasterol on the thermotropic phase behavior and organization of dipalmitoylphosphatidylcholine bilayer membranes.
We performed comparative DSC and FTIR spectroscopic measurements of the effects of β-sitosterol (Sito) and stigmasterol (Stig) on the thermotropic phase behavior and organization of DPPC bilayers. Sito and Stig are the major sterols in the biological membranes of higher plants, whereas cholesterol (Chol) is the major sterol in mammalian membranes. Sito differs in structure from Chol in having an ethyl group at C24 of the alkyl side-chain, and Stig in having both the C24 ethyl group and trans-double bond at C22. Our DSC studies indicate that the progressive incorporation of Sito and Stig decrease the temperature and cooperativity of the pretransition of DPPC to a slightly lesser and greater extent than Chol, respectively, but the pretransition persists to 10 mol % sterol concentration in all cases. All three sterols produce essentially identical effects on the thermodynamic parameters of the sharp component of the DPPC main phase transition. However, the ability to increase the temperature and decrease the cooperativity and enthalpy of the broad component decreases in the order Chol>Sito>Stig. Nevertheless, at higher Sito/Stig concentrations, there is no evidence of sterol crystallites. Our FTIR spectroscopic studies demonstrate that Sito and especially Stig incorporation produces a smaller ordering of the hydrocarbon chains of fluid DPPC bilayers than does Chol. In general, the presence of a C24 ethyl group in the alkyl side-chain reduces the characteristic effects of Chol on the thermotropic phase behavior and organization of DPPC bilayer membranes, and a trans-double bond at C22 magnifies this effect.
Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7.
David A
DA
Mannock
Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7.
Matthew G K
MG
Benesch
Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7.
Ruthven N A H
RN
Lewis
Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7. Electronic address: rmcelhan@ualberta.ca.
Ronald N
RN
McElhaney
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry* / Q000378:metabolism; D002152:Calorimetry, Differential Scanning; D002775:Cholestadienols / Q000737:chemistry; D002784:Cholesterol / Q000031:analogs & derivatives / Q000737:chemistry* / Q000378:metabolism; D008051:Lipid Bilayers; D015394:Molecular Structure; D044367:Phase Transition; D010840:Phytosterols / Q000737:chemistry; D012855:Sitosterols / Q000737:chemistry* / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D013265:Stigmasterol / Q000737:chemistry* / Q000378:metabolism; D013696:Temperature
D002775:Cholestadienols; D008051:Lipid Bilayers; D010840:Phytosterols; D012855:Sitosterols; D015060:1,2-Dipalmitoylphosphatidylcholine; C003532:brassicasterol; C021273:campesterol; C025473:gamma-sitosterol; D002784:Cholesterol; D013265:Stigmasterol
Brassicasterol; Campesterol; Cholesterol; Sterol-phospholipid interactions; Stigmasterol; β-sitosterol