Purification and characterization of four antibacterial peptides from protamex hydrolysate of Atlantic mackerel (Scomber scombrus) by-products.
Proteins from fish by-product sources are valuable source of bioactive peptides and show promise as functional foods ingredients. The objective of the present study was to isolate and characterize antibacterial peptides from protamex hydrolysates of Atlantic mackerel (Scomber scombrus) by-products. Four sequences SIFIQRFTT (P4), RKSGDPLGR (P8.1), AKPGDGAGSGPR (P8.2) and GLPGPLGPAGPK (P11) were identified in peptide fractions separated using RP-HPLC. At 200 μg mL(-1), while peptides P8.1, P8.2 and P11 exhibited partial inhibition, P4 totally inhibited tested Gram-positive (Listeria innocua) and Gram-negative (Escherichia coli) bacterial strains. These results suggest that the protein hydrolysate derived from mackerel by-products could be used as an antimicrobial ingredient in both functional food and nutraceutical applications.
STELA Dairy Research Centre, Institute of Nutrition and Functional Foods, Université Laval, Québec, QC G1V 0A6, Canada.
Nadia
N
Ennaas
STELA Dairy Research Centre, Institute of Nutrition and Functional Foods, Université Laval, Québec, QC G1V 0A6, Canada. Electronic address: riadh.hammami@fsaa.ulaval.ca.
Riadh
R
Hammami
STELA Dairy Research Centre, Institute of Nutrition and Functional Foods, Université Laval, Québec, QC G1V 0A6, Canada; Department of Biology, Chemistry and Geography, Université du Québec à Rimouski (UQAR), 300 Allée des Ursulines, Rimouski, QC G5L 3A1, Canada.
Lucie
L
Beaulieu
STELA Dairy Research Centre, Institute of Nutrition and Functional Foods, Université Laval, Québec, QC G1V 0A6, Canada. Electronic address: ismail.fliss@fsaa.ulaval.ca.
Ismail
I
Fliss
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects; D029941:Fish Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D006868:Hydrolysis; D008087:Listeria / Q000187:drug effects; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D010473:Perciformes / Q000235:genetics / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D029941:Fish Proteins; D010446:Peptide Fragments
Amino acids; Antimicrobial peptide; Escherichia coli; Listeria; Mackerel by-products; Purification