Ancient mitochondrial DNA analyses of ascaris eggs discovered in coprolites from joseon tomb.
Analysis of ancient DNA (aDNA) extracted from Ascaris is very important for understanding the phylogenetic lineage of the parasite species. When aDNAs obtained from a Joseon tomb (SN2-19-1) coprolite in which Ascaris eggs were identified were amplified with primers for cytochrome b (cyt b) and 18S small subunit ribosomal RNA (18S rRNA) gene, the outcome exhibited Ascaris specific amplicon bands. By cloning, sequencing, and analysis of the amplified DNA, we obtained information valuable for comprehending genetic lineage of Ascaris prevalent among pre-modern Joseon peoples.
Bioanthropology and Paleopathology Lab, Institute of Forensic Science, Seoul National University College of Medicine, Seoul 110-799, Korea.
Chang Seok
CS
Oh
Department of Parasitology and Research Center for Mummy, Dankook University, Cheonan 330-715, Korea.
Min
M
Seo
Bioanthropology and Paleopathology Lab, Institute of Forensic Science, Seoul National University College of Medicine, Seoul 110-799, Korea.
Jong Ha
JH
Hong
Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 110-799, Korea.
Jong-Yil
JY
Chai
Hangang Institute of Cultural Heritage, Seoul 143-904, Korea.
Seung Whan
SW
Oh
Sangmyung University, Seoul 110-743, Korea.
Jun Bum
JB
Park
Bioanthropology and Paleopathology Lab, Institute of Forensic Science, Seoul National University College of Medicine, Seoul 110-799, Korea.
Dong Hoon
DH
Shin
D000328:Adult; D000818:Animals; D001196:Ascariasis / Q000175:diagnosis / Q000266:history / Q000469:parasitology*; D001200:Ascaris / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D045303:Cytochromes b / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D018509:DNA, Helminth / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics* / Q000266:history; D005260:Female; D005580:Fossils / Q000266:history / Q000469:parasitology; D049690:History, Ancient; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009106:Mummies / Q000266:history / Q000469:parasitology*; D010063:Ovum / Q000737:chemistry / Q000145:classification; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics
D017931:DNA Primers; D018509:DNA, Helminth; D004272:DNA, Mitochondrial; D012337:RNA, Ribosomal, 18S; D045303:Cytochromes b
18S rRNA; Ascaris; Korean mummy; ancient DNA; cytochrome b