[Expression of inulinase genes in the yeasts Saccharomyces cerevisiae and Kluyveromyces marxianus].
Expression of the genes encoding the enzymes involved in inulin, sucrose, and glucose metabolism in the yeasts Saccharomyces cerevisiae and Kluyveromyces marxianus was studied. The exon-intron structure of the relevant genes was identified and the primers for quantitative PCR were optimized. Expression of the genes was found to depend on the carbon source. Glucose was shown to exhibit a repressive effect on inulinase synthesis by K. marxianus, while in S. cerevisiae glucose and sucrose were inulinase inducer and repressor, respectively.
G G
GG
Sokolenko
N A
NA
Karpechenko
D017931:DNA Primers / Q000138:chemical synthesis; D005091:Exons; D005285:Fermentation; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D005947:Glucose / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000096:biosynthesis / Q000235:genetics*; D007438:Introns; D007444:Inulin / Q000378:metabolism; D007700:Kinetics; D007716:Kluyveromyces / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism*
D017931:DNA Primers; D005656:Fungal Proteins; D013395:Sucrose; D007444:Inulin; D006026:Glycoside Hydrolases; C023086:inulinase; D005947:Glucose