Enantioselective separation and determination of the dinotefuran enantiomers in rice, tomato and apple by HPLC.
An effective chiral analytical method was developed for the resolution and determination of dinotefuran enantiomers in rice, tomato and apple samples. Dinotefuran enantiomers were baseline-separated and determined on a novel chiral column, ChromegaChiral CCA, with n-hexane-ethanol-methanol (85:5:10, v/v/v) as the mobile phase at a flow rate of 1.0 mL/min with UV detection at 270 nm. The resolution of dinotefuran enantiomers was about 1.8. The first eluted enantiomer was (+)-dinotefuran and the second eluted one was (-)-dinotefuran. The effects of mobile-phase composition and column temperature on the enantioseparation were evaluated. The method was validated for linearity, repeatability, accuracy, LOD and LOQ. LOD was 0.15 mg/kg in rice and tomato, 0.05 mg/kg in apple, with an LOQ of 0.5 mg/kg in rice and tomato, 0.2 mg/kg in apple. The average recoveries of the pesticide from all matrices ranged from 75.8 to 92.9% for all fortification levels The precision values associated with the analytical method, expressed as RSD values, were <16.5% for the pesticide in all matrices. The methodology was successfully applied for the enantioselective analysis of dinotefuran enantiomers in real samples, indicating its efficiency in investigating the environmental stereochemistry of dinotefuran in food matrix.
Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Key Laboratory of Pesticide Chemistry and Application, Ministry of Agriculture, Beijing, P. R. China. chenxiu_88@126.com
Xiu
X
Chen
Fengshou
F
Dong
Xingang
X
Liu
Jun
J
Xu
Jing
J
Li
Yuanbo
Y
Li
Yunhao
Y
Wang
Yongquan
Y
Zheng
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D006146:Guanidines / Q000032:analysis* / Q000737:chemistry*; D006801:Humans; D007306:Insecticides / Q000032:analysis* / Q000737:chemistry*; D057230:Limit of Detection; D018551:Solanum lycopersicum / Q000737:chemistry; D027845:Malus / Q000737:chemistry; D000073943:Neonicotinoids; D009574:Nitro Compounds / Q000032:analysis* / Q000737:chemistry*; D012275:Oryza / Q000737:chemistry; D010573:Pesticide Residues / Q000032:analysis / Q000737:chemistry; D013237:Stereoisomerism
D006146:Guanidines; D007306:Insecticides; D000073943:Neonicotinoids; D009574:Nitro Compounds; D010573:Pesticide Residues; C465368:dinotefuran
Chiral pesticides; Dinotefuran; Enantioseparation; Food; Residue