Functionalized nano-graphene oxide particles for targeted fluorescence imaging and photothermy of glioma U251 cells.
OBJECTIVE
This study was to prepare the functionalized nano-graphene oxide (nano-GO) particles, and observe targeted fluorescence imaging and photothermy of U251 glioma cells under near infrared (NIR) exposure.


METHODS
The functionalized nano-GO-Tf-FITC particles were prepared and then were incubated with U251 glioma cells. Estimation of CCK8 cell activity was adopted for measurement of cytotoxicity. The effect of fluorescein imaging was detected by fluorescence microscope with anti-CD71-FITC as a control. Finally, we detected the killing efficacy with flow cytometry after an 808 nm NIR exposure.


RESULTS
Both nano-GO-Tf-FITC group and CD71-FITC group exhibited green-yellow fluorescence, while the control group without the target molecule nano-GO-FITC was negative. The nano-GO-Tf-FITC was incubated with U251 cells at 0.1 mg/ml, 1.0 mg/ml, 3.0 mg/ml and 5.0 mg/ml. After 48 h of incubation, the absorbance was 0.747 ± 0.031, 0.732 ± 0.043, 0.698 ± 0.051 and 0.682 ± 0.039, while the absorbance of control group is 0.759 ± 0.052. There is no significant difference between the nano-GO-FITC groups and control group. In addition, the apoptosis and death index of nano-GO-Tf-FITC group was significantly higher than that of nano-GO-FITC and blank control group (P < 0.05).


CONCLUSIONS
The nano-GO-Tf-FITC particles with good biological compatibility and low cytotoxicity are successfully made, which have an observed effect of target imaging and photothermal therapy on glioma U251 cells.
Department of Neurosurgery, The Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University Guangzhou 510120, China.
Zhong-Jun
ZJ
Li
Department of Neurosurgery, The Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University Guangzhou 510120, China.
Chao
C
Li
Department of Neurosurgery, The Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University Guangzhou 510120, China.
Mei-Guang
MG
Zheng
Polymer Chemistry Institute of Sun Yat-Sen University Guangzhou 510275, China.
Jia-Dong
JD
Pan
Polymer Chemistry Institute of Sun Yat-Sen University Guangzhou 510275, China.
Li-Ming
LM
Zhang
Department of Neurosurgery, The Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University Guangzhou 510120, China.
Yue-Fei
YF
Deng
Glioma; fluorescence imaging; graphene oxide; photothermy; transferring