Combination of selective enrichment and MALDI-TOF MS for rapid detection of Streptococcus agalactiae colonisation of pregnant women.
Sample preparation was optimized for MALDI-TOF MS directly from selective enrichment broth to detect Streptococcus agalactiae. The method was tested on 100 vaginal samples of pregnant women; positive and negative predictive values were 100 and 91%, respectively. If it indicates positivity, colonisation can be reported 18-24h after sample collection.
Institute of Clinical Microbiology, University of Szeged, Szeged, Hungary. Electronic address: mpabrok@gmail.com.
Marianna
M
Ábrók
Institute of Clinical Microbiology, University of Szeged, Szeged, Hungary.
Ágnes
Á
Arcson
Institute of Clinical Microbiology, University of Szeged, Szeged, Hungary.
Andrea
A
Lázár
Institute of Clinical Microbiology, University of Szeged, Szeged, Hungary.
Edit
E
Urbán
Institute of Clinical Microbiology, University of Szeged, Szeged, Hungary.
Judit
J
Deák
D001431:Bacteriological Techniques / Q000379:methods*; D005260:Female; D006801:Humans; D011237:Predictive Value of Tests; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis* / Q000382:microbiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013290:Streptococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013292:Streptococcus agalactiae / Q000737:chemistry / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification*; D013997:Time Factors; D014621:Vagina / Q000382:microbiology
Group B Streptococcus; MALDI-TOF MS; Pregnancy; Sample preparation; Screening; Todd-Hewitt broth