A fluorimetric study on the interaction between a Trp-containing beta-strand peptide and amphiphilic polymer-coated gold nanoparticles.
Owing to the inevitability of nanoparticles encountering proteins/peptides in current bio-nano-medicine development, it is important to know how they interact with each other in vitro before developing in vivo applications. To this end, a model de novo β-sheet-forming peptide and typical biocompatible nanoparticles were selected to study thermodynamic aspects of their interactions via a fluorescence quenching method. The results showed that Pep11 and AuNPs spontaneously formed conjugates, mainly driven by a coulombic interaction with a binding affinity of ~ 0.1 µM(-1); the physical adsorption process was cooperative. These results deepen our quantitative understanding of nanoparticle-peptide interactions. The results may also be helpful in further nanoparticle-peptide hybrid nanofabrication and also useful for the application of nanoparticles in the treatment of amyloid diseases.
School of Life Science, Inner Mongolia Agricultural University, Hohhot, China.
Ming
M
Yuan
School of Life Science, Inner Mongolia Agricultural University, Hohhot, China.
Ruibo
R
Zhong
School of Life Science, Inner Mongolia Agricultural University, Hohhot, China.
Xiaoling
X
Yun
School of Life Science, Inner Mongolia Agricultural University, Hohhot, China.
Jiahua
J
Hou
School of Life Science, Inner Mongolia Agricultural University, Hohhot, China.
Qiqige
Q
Du
School of Life Science, Inner Mongolia Agricultural University, Hohhot, China.
Guofen
G
Zhao
School of Life Science, Inner Mongolia Agricultural University, Hohhot, China.
Feng
F
Zhang
D005470:Fluorometry; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D010455:Peptides / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry*; D013816:Thermodynamics; D014364:Tryptophan / Q000737:chemistry*
D010455:Peptides; D011108:Polymers; D013501:Surface-Active Agents; D006046:Gold; D014364:Tryptophan
fluorescence; gold nanoparticle; interaction; peptide; quenching