Assessment of Reproducibility of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Bacterial and Yeast Identification.
Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has revolutionized the identification of clinical bacterial and yeast isolates. However, data describing the reproducibility of MALDI-TOF MS for microbial identification are scarce. In this study, we show that MALDI-TOF MS-based microbial identification is highly reproducible and can tolerate numerous variables, including differences in testing environments, instruments, operators, reagent lots, and sample positioning patterns. Finally, we reveal that samples of bacterial and yeast isolates prepared for MALDI-TOF MS identification can be repeatedly analyzed without compromising organism identification.
Department of Pathology, Children's Healthcare of Atlanta, Atlanta, Georgia, USA Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia, USA Department of Pathology and Laboratory Medicine, Washington University in St. Louis School of Medicine, St. Louis, Missouri, USA.
Lars F
LF
Westblade
Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, California, USA.
Omai B
OB
Garner
bioMérieux, Inc., Durham, North Carolina, USA.
Karen
K
MacDonald
bioMérieux, Inc., Hazelwood, Missouri, USA.
Constance
C
Bradford
bioMérieux, Inc., Hazelwood, Missouri, USA.
David H
DH
Pincus
Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, California, USA Division of Infectious Diseases, Laboratory Sciences of Arizona/Sonora Quest Laboratories, Phoenix, Arizona, USA Department of Pathology, University of Arizona College of Medicine, Phoenix, Arizona, USA.
A Brian
AB
Mochon
Barnes-Jewish Hospital, St. Louis, Missouri, USA.
Rebecca
R
Jennemann
Department of Pathology and Laboratory Medicine, North Shore-LIJ Laboratories, Lake Success, New York, USA.
Ryhana
R
Manji
Department of Pathology and Laboratory Medicine, North Shore-LIJ Laboratories, Lake Success, New York, USA.
Maureen
M
Bythrow
Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, California, USA Roche Molecular Systems, Inc., Pleasanton, California, USA.
Michael A
MA
Lewinski
Department of Pathology and Laboratory Medicine, Washington University in St. Louis School of Medicine, St. Louis, Missouri, USA.
Carey-Ann D
http://orcid.org/0000-0002-1137-840X
CA
Burnham
bioMérieux, Inc., Durham, North Carolina, USA Hofstra North Shore-LIJ School of Medicine, Hempstead, NY, USA christine.ginocchio@biomerieux.com.
Christine C
CC
Ginocchio
D001419:Bacteria / Q000737:chemistry* / Q000145:classification*; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods*; D015203:Reproducibility of Results; D013048:Specimen Handling / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D015003:Yeasts / Q000737:chemistry* / Q000145:classification*