[Effect of IL-1β on inflammatory factors synthesis of human gingival fibroblasts attached to different titaniums].
OBJECTIVE
To evaluate the effects of IL-1β on inflammatory factors synthesis of human gingival fibroblasts on different surfaces of titaniums in vitro.


METHODS
Human gingival fibroblasts were cultured on 3 different kinds of titaniums. The cells were stimulated with IL-1β, and the IL-6 and IL-8 production was detected by polymerase chain reaction (PCR) after 3, 6 and 24 h. The date was statistically analyzed with SPSS13.0 software package.


RESULTS
A significant increase of the expression of gingival fibroblasts to inflammatory factors (IL-6, IL-8) after IL-1β was noted; PCR results of the production of inflammatory factors in gingival fibroblasts with the intervention of IL-1β showed that the production of inflammatory factors (IL-6, IL-8) on titanium and titanium alloy were less than that on the titanium nitride.


CONCLUSIONS
IL-1β can stimulate high expression of inflammatory factors; The production of IL-6 and IL-8 on titanium and titanium alloy are less, suggesting that implant made of titanium and titanium alloy can reduce the incidence of peri-implant inflammation, and improve the successful rate of implantation.
Department of Prosthodontic, Affiliated Hospital to Binzhou Medical College. Binzhou 256603, Shandong Province, China.E-mail:544242395@qq.com.
Zhi-xin
ZX
Sun
Yun-tao
YT
Zhang
D002478:Cells, Cultured; D005347:Fibroblasts; D005881:Gingiva; D006801:Humans; D053583:Interleukin-1beta; D015850:Interleukin-6; D016209:Interleukin-8; D014025:Titanium
D053583:Interleukin-1beta; D015850:Interleukin-6; D016209:Interleukin-8; C041500:titanium nitride; D014025:Titanium