Probing actin filament and binding protein interaction using an atomic force microscopy.
Actin filaments play essential roles in many kinds of cellular functions by interacting with hundreds of actin binding proteins. Here we probe the interaction between actin filament and a binding protein, α-actinin, using an atomic force microscopy (AFM) and dynamic force spectroscopy (DFS). The distribution of rupturing events including specific and non-specific interactions of actin filament/α- actinin and BSA/α-actinin were analyzed. The rupture force of the actin filament/α-actinin binding was significantly larger than that of the BSA/α-actinin non-specific interaction, and the peaks represent typical multiple parallel bonds. In addition, based on the rupture forces in different loading rate DFS experiments, the dissociation constant of actin filament/α-actinin binding was estimated. The value is in good agreement with a previously reported value obtained by optical tweezer measurement. We expect that the present method will be useful for interaction measurement of actin filaments and many kinds of binding protein.
Sung-Woong
SW
Han
Kyohei
K
Morita
Patriche
P
Simona
Takanori
T
Kihara
Jun
J
Miyake
Mihaela
M
Banu
Taiji
T
Adachi
D008841:Actin Cytoskeleton / Q000737:chemistry* / Q000378:metabolism; D018625:Microscopy, Atomic Force / Q000379:methods*; D015335:Molecular Probes; D011485:Protein Binding
D015335:Molecular Probes