[The effects of endothelin 1 and an inhibitor of phosphorylation of IκB on the content of free sulfhydryl groups (-SH) and protein in rat kidney].
Endothelin (ET)-1 is a potent vasoconstrictor. ET-1 an increase in the synthesis of transcription factor NF-κB, which is responsible for the production of reactive oxygen species (ROS), and cytokines.


OBJECTIVE
The aim of the study was to determine whether blocking NF-κB pathway affects the content of free -SH groups and total protein in rat kidney homogenates.


METHODS
Experiments were carried out on four groups of male rats of Wistar-Kyoto strain. Group I - control was administered into the femoral vein solution of 0.9 % NaCl; in group II and III - was given endothelin-1 at doses of 1.5 and 12.5 μg/kg b.w., group IV - was given BAY 11-7082 (inhibitor of phosporylation of IκBα) at a dose of 10 mg/kg b.w. 30 min prior to administration of ET-1 at a dose of 12.5 μg/kg b.w.


RESULTS
Administration of ET-1 at doses of 1.5 and 12.5 μg/kg b.w. resulted in a statistically significant reduction of free -SH groups and protein in kidney homogenates compared to the control group. In contrast, administration of an inhibitor of nuclear factor NF-κB (BAY 11-7082 (10 mg/kg b.w.) during the oxidative stress induced by ET-1 (12.5 μg/kg b.w.) significantly increased the concentration of free -SH groups (p <0.001) and protein (p <0.01) in the kidney.


CONCLUSIONS
ET-1 caused dose-dependent significant reduction of concentration free -SH groups and total protein. A inhibitor of phosphorylation of IκBα (BAY 11-7082) given during the oxidative stress induced by ET-1 (12.5 μg/kg b.w.) significantly increased the concentration of free -SH groups and protein in the kidney tissue.
Medical University of Lodz, Poland: Chair of Experimental and Clinical Physiology, Department of Cardiovascular Physiology.
Anna
A
Gorąca
Medical University of Lodz, Poland: Chair of Applied Pharmacy, Department of Pharmacy.
Beata
B
Skibska
D000818:Animals; D004305:Dose-Response Relationship, Drug; D019332:Endothelin-1 / Q000494:pharmacology*; D020672:I-kappa B Proteins / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D009570:Nitriles / Q000494:pharmacology*; D018384:Oxidative Stress; D010766:Phosphorylation / Q000187:drug effects; D051381:Rats; D011921:Rats, Inbred WKY; D017382:Reactive Oxygen Species / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D013450:Sulfones / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
C434003:3-(4-methylphenylsulfonyl)-2-propenenitrile; D019332:Endothelin-1; D020672:I-kappa B Proteins; D016328:NF-kappa B; D009570:Nitriles; D017382:Reactive Oxygen Species; D013438:Sulfhydryl Compounds; D013450:Sulfones; D014409:Tumor Necrosis Factor-alpha
NF-κB blocking; SH-groups; endothelin 1; oxidative stress