A simple assay for glutathione in whole blood.
A method for detecting glutathione selectively in whole blood deposited on filter paper is described. GSH is fractionated from proteins, hemoglobin and other potentially interfering components and determined using a resorufin-acrylate fluorescent probe. The relative standard deviation is lower than 5% (n = 5). Recoveries of GSH from whole blood are between 94% and 108.6%.
Department of Chemistry, Portland State University, Portland, OR 97201, USA. strongin@pdx.edu.
Lovemore
L
Hakuna
Brandon
B
Doughan
Jorge O
JO
Escobedo
Robert M
RM
Strongin
D001774:Blood Chemical Analysis / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D005978:Glutathione / Q000097:blood* / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010078:Oxazines / Q000737:chemistry
D005456:Fluorescent Dyes; D010078:Oxazines; C014180:resorufin; D005978:Glutathione