Hemodynamic Characterization of a Mouse Model for Investigating the Cellular and Molecular Mechanisms of Neotissue Formation in Tissue-Engineered Heart Valves.
Decellularized allograft heart valves have been used as tissue-engineered heart valve (TEHV) scaffolds with promising results; however, little is known about the cellular mechanisms underlying TEHV neotissue formation. To better understand this phenomenon, we developed a murine model of decellularized pulmonary heart valve transplantation using a hemodynamically unloaded heart transplant model. Furthermore, because the hemodynamics of blood flow through a heart valve may influence morphology and subsequent function, we describe a modified loaded heterotopic heart transplant model that led to an increase in blood flow through the pulmonary valve. We report host cell infiltration and endothelialization of implanted decellularized pulmonary valves (dPV) and provide an experimental approach for the study of TEHVs using mouse models.
1 Tissue Engineering and Surgical Research, The Research Institute at Nationwide Children's Hospital , Columbus, Ohio.
Iyore A
IA
James
1 Tissue Engineering and Surgical Research, The Research Institute at Nationwide Children's Hospital , Columbus, Ohio.
Tai
T
Yi
1 Tissue Engineering and Surgical Research, The Research Institute at Nationwide Children's Hospital , Columbus, Ohio.
Shuhei
S
Tara
1 Tissue Engineering and Surgical Research, The Research Institute at Nationwide Children's Hospital , Columbus, Ohio.
Cameron A
CA
Best
2 The Ohio State University College of Medicine , Columbus, Ohio.
Alexander J
AJ
Stuber
2 The Ohio State University College of Medicine , Columbus, Ohio.
Kejal V
KV
Shah
3 Center for Cardiovascular and Pulmonary Research, The Research Institute at Nationwide Children's Hospital , Columbus, Ohio.
Blair F
BF
Austin
1 Tissue Engineering and Surgical Research, The Research Institute at Nationwide Children's Hospital , Columbus, Ohio.
Tadahisa
T
Sugiura
1 Tissue Engineering and Surgical Research, The Research Institute at Nationwide Children's Hospital , Columbus, Ohio.
Yong-Ung
YU
Lee
3 Center for Cardiovascular and Pulmonary Research, The Research Institute at Nationwide Children's Hospital , Columbus, Ohio.
Joy
J
Lincoln
3 Center for Cardiovascular and Pulmonary Research, The Research Institute at Nationwide Children's Hospital , Columbus, Ohio.
Aaron J
AJ
Trask
1 Tissue Engineering and Surgical Research, The Research Institute at Nationwide Children's Hospital , Columbus, Ohio.
Toshiharu
T
Shinoka
1 Tissue Engineering and Surgical Research, The Research Institute at Nationwide Children's Hospital , Columbus, Ohio.
Christopher K
CK
Breuer
D000818:Animals; D016027:Heart Transplantation; D006350:Heart Valve Prosthesis; D006351:Heart Valves / Q000000981:diagnostic imaging / Q000502:physiology*; D006352:Heart Ventricles; D006439:Hemodynamics; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D011312:Pressure; D011664:Pulmonary Valve / Q000166:cytology / Q000502:physiology; D023822:Tissue Engineering / Q000379:methods*; D014463:Ultrasonography