Co-delivery of doxorubicin and tumor-suppressing p53 gene using a POSS-based star-shaped polymer for cancer therapy.
In this work, a star-shaped polymer consisting of a cationic poly[2-(dimethylamino) ethyl methacrylate] (PDMAEMA) shell and a zwitterionic poly[N-(3-(methacryloylamino) propyl)-N,N-dimethyl-N-(3-sulfopropyl) ammonium hydroxide] (PMPD) corona was grafted from a polyhedral oligomeric silsesquioxanes (POSS)-based initiator via atomic transfer radical polymerization (ATRP). The reported star-shaped polymer could form stable micelles in aqueous solutions even in the presence of serum. In addition, anti-cancer drug doxorubicin and tumor-suppressing p53 gene were loaded in the process of micelle formation. The formed polyplex was biocompatible and highly efficient for both drug and gene delivery. Furthermore, the polyplex was able to cause a high apoptotic rate of tumor cells both in vitro and in vivo. This combination delivery strategy offers a promising method for cancer therapy and can be used for further clinical applications.
School of Materials Science and Engineering, Tianjin Key Laboratory of Composite and Functional Materials, Tianjin University, Tianjin 300072, PR China.
Yongmao
Y
Li
School of Materials Science and Engineering, Tianjin Key Laboratory of Composite and Functional Materials, Tianjin University, Tianjin 300072, PR China.
Bing
B
Xu
School of Materials Science and Engineering, Tianjin Key Laboratory of Composite and Functional Materials, Tianjin University, Tianjin 300072, PR China.
Tao
T
Bai
School of Materials Science and Engineering, Tianjin Key Laboratory of Composite and Functional Materials, Tianjin University, Tianjin 300072, PR China. Electronic address: wgliu@tju.edu.cn.
Wenguang
W
Liu
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage; D017209:Apoptosis; D016466:CHO Cells; D019556:COS Cells; D002412:Cations; D002453:Cell Cycle / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003412:Cricetulus; D004317:Doxorubicin / Q000008:administration & dosage*; D004337:Drug Carriers / Q000737:chemistry*; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D008689:Methacrylates / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008823:Micelles; D008856:Microscopy, Fluorescence; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000188:drug therapy*; D009757:Nylons / Q000737:chemistry*; D017646:Organosilicon Compounds / Q000737:chemistry*; D011109:Polymethacrylic Acids / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
D000903:Antibiotics, Antineoplastic; D002412:Cations; D004337:Drug Carriers; D008689:Methacrylates; D008823:Micelles; D009757:Nylons; D017646:Organosilicon Compounds; D011109:Polymethacrylic Acids; D000644:Quaternary Ammonium Compounds; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; C407037:poly(2-(dimethylamino)ethyl methacrylate); C521417:polyhedraloligosilsesquioxane; D004317:Doxorubicin
Amphiphilic micelles; Cancer therapy; Co-delivery; Doxorubicin; P53; POSS