Analysis of expression and function of the co-stimulatory receptor SLAMF1 in immune cells from patients with systemic lupus erythematosus (SLE).
The signaling lymphocytic activation molecule SLAMF1 (CD150) is a co-stimulatory molecule that is expressed by most immune cells, including T regulatory (Treg) lymphocytes. Since different abnormalities have been reported regarding the number and function of Foxp3+ Treg cells in patients with systemic lupus erythematosus (SLE), we decided to analyze the expression and function of CD150 in these regulatory lymphocytes in this condition. We isolated peripheral blood mononuclear cells from 20 patients with SLE, and 20 healthy controls. The expression of SLAMF1 was determined by multi-parametric flow cytometry and the suppressive function of CD4+CD25+ lymphocytes, upon engagement or not of CD150 with an agonistic monoclonal antibody, was analyzed by an assay of inhibition of cell proliferation. We observed a significantly increased expression of SLAMF1 by CD3+CD4+ helper T cells and CD19+ B cells in patients with SLE and active disease. However, similar levels of SLAMF1 expression were detected in Foxp3+ Treg cells from patients and controls. In contrast, a higher proportion of SLE patients increased their suppressive function of Treg cells upon CD150 engagement compared to healthy controls. Our data suggest that SLAMF1 is another significant piece in the intricate defective immune-regulatory function of patients with SLE.
Department of Immunology, UASLP, San Luis Potosí, Mexico.
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Liñán-Rico
Department of Immunology, UASLP, San Luis Potosí, Mexico.
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Hernández-Castro
Unidad Académica Multidisciplinaria Zona Huasteca, UASLP, San Luis Potosí, Mexico.
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Doniz-Padilla
Unidad Académica Multidisciplinaria Zona Huasteca, UASLP, San Luis Potosí, Mexico.
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Portillo-Salazar
Department of Immunology, UASLP, San Luis Potosí, Mexico Regional Unit of Rheumatology and Osteoporosis, Hospital Central Dr. Ignacio Morones Prieto, San Luis Potosí, Mexico.
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Baranda
School of Medicine, UAEM, Cuernavaca, Mexico.
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Cruz-Muñoz
Department of Immunology, UASLP, San Luis Potosí, Mexico Unidad Académica Multidisciplinaria Zona Huasteca, UASLP, San Luis Potosí, Mexico rgonzale@uaslp.mx.
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González-Amaro
D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000096:biosynthesis / Q000276:immunology*; D015551:Autoimmunity / Q000276:immunology; D048708:Cell Growth Processes / Q000276:immunology; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D051858:Forkhead Transcription Factors / Q000276:immunology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008180:Lupus Erythematosus, Systemic / Q000188:drug therapy / Q000276:immunology*; D008875:Middle Aged; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000276:immunology*; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D055815:Young Adult
D015703:Antigens, CD; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D011956:Receptors, Cell Surface; C000604059:SLAMF1 protein, human; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1
CD150; autoimmunity; immune receptors; regulatory T cells