A simple and rapid label-free fluorimetric biosensor for protamine detection based on glutathione-capped CdTe quantum dots aggregation.
A novel fluorescent biosensor is developed, based on glutathione-capped CdTe quantum dots aggregation, for the determination of trace amount of an important drug, protamine. In this method with increasing the protamine concentration, the fluorescence of the quantum dots was quenched due to their aggregation. Different parameters affect the sensitivity, such as pH and the amount of the quantum dots, were optimized. Using the new optical biosensor, under the optimized conditions, protamine could be measured in the range of 2.0-200 ng mL(-1) with a detection limit of 1.0 ng mL(-)(1). The relative standard deviation for five replicates determination of 30.0 ng mL(-)(1) protamine was 1.26%. The influence of common interfering species on the protamine detection was studied. The results showed that the biosensor is highly selective and sensitive for the detection of protamine. The optical biosensor was successfully used for the determination of protamine in real samples.
Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran. Electronic address: Ensafi@cc.iut.ac.ir.
Ali A
AA
Ensafi
Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran.
N
N
Kazemifard
Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran.
B
B
Rezaei
D015374:Biosensing Techniques / Q000379:methods*; D019187:Cadmium Compounds / Q000737:chemistry*; D005978:Glutathione / Q000737:chemistry*; D006494:Heparin Antagonists / Q000032:analysis / Q000097:blood*; D006801:Humans; D057230:Limit of Detection; D011479:Protamines / Q000032:analysis / Q000097:blood*; D045663:Quantum Dots / Q000737:chemistry* / Q000648:ultrastructure; D013050:Spectrometry, Fluorescence / Q000379:methods; D013691:Tellurium / Q000737:chemistry*
D019187:Cadmium Compounds; D006494:Heparin Antagonists; D011479:Protamines; D005978:Glutathione; D013691:Tellurium; C028337:cadmium telluride
Aggregation assay; Fluorescence biosensor; Glutathione-capped CdTe quantum dot; Protamine