Antiproliferative and Apoptosis-Inducing Activities of 4-Isopropyl-2,6-bis(1-phenylethyl)phenol Isolated from Butanol Fraction of Cordyceps bassiana.
The Cordyceps species have been widely used for treating various cancer diseases. Although the Cordyceps species have been widely known as an alternative anticancer remedy, which compounds are responsible for their anticancer activity is not fully understood. In this study, therefore, we examined the anticancer activity of 5 isolated compounds derived from the butanol fraction (Cb-BF) of Cordyceps bassiana. For this purpose, several cancer cell lines such as C6 glioma, MDA-MB-231, and A549 cells were employed and details of anticancer mechanism were further investigated. Of 5 compounds isolated by activity-guided fractionation from BF of Cb-EE, KTH-13, and 4-isopropyl-2,6-bis(1-phenylethyl)phenol, Cb-BF was found to be the most potent antiproliferative inhibitor of C6 glioma and MDA-MB-231 cell growth. KTH-13 treatment increased DNA laddering, upregulated the level of Annexin V positive cells, and altered morphological changes of C6 glioma and MDA-MB-231 cells. In addition, KTH-13 increased the levels of caspase 3, caspase 7, and caspase 9 cleaved forms as well as the protein level of Bax but not Bcl-2. It was also found that the phosphorylation of AKT and p85/PI3K was also clearly reduced by KTH-13 exposure. Therefore, our results suggest KTH-13 can act as a potent antiproliferative and apoptosis-inducing component from Cordyceps bassiana, contributing to the anticancer activity of this mushroom.
Department of Genetic Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
Ji Hye
JH
Kim
Department of Chemistry, Kwangwoon University, Seoul 139-701, Republic of Korea.
Yunmi
0000-0003-1315-4001
Y
Lee
Institute for Bio-Medical Convergence, International St. Mary's Hospital and College of Medicine, Catholic Kwandong University, Incheon 404-834, Republic of Korea.
Gi-Ho
GH
Sung
Department of Genetic Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
Han Gyung
HG
Kim
Department of Genetic Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
Deok
0000-0001-9092-9662
D
Jeong
Department of Genetic Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
Jae Gwang
JG
Park
Department of Genetic Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
Kwang-Soo
KS
Baek
Department of Genetic Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
Nak Yoon
0000-0001-8814-8701
NY
Sung
Department of Genetic Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
Sungjae
0000-0002-5513-5135
S
Yang
Department of Biochemistry, Kangwon National University, Chuncheon 220-700, Republic of Korea.
Deok Hyo
DH
Yoon
Department of Life Science, Gachon University, Seongnam, Kyeonggi-do 461-701, Republic of Korea.
Sang Yeol
SY
Lee
College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 702-701, Republic of Korea.
Hyojeung
H
Kang
Department of Chemistry, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
Changsik
C
Song
Mushroom Research Division, Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA, Suwon 441-707, Republic of Korea.
Jae Han
JH
Cho
Mushroom Research Division, Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA, Suwon 441-707, Republic of Korea.
Kang-Hyo
KH
Lee
Department of Biochemistry, Kangwon National University, Chuncheon 200-701, Republic of Korea.
Tae Woong
TW
Kim
Department of Genetic Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
Jae Youl
0000-0001-8141-9927
JY
Cho