A dynein independent role of Tctex-1 at the kinetochore.
Dynein light chains are accessory subunits of the cytoplasmic dynein complex, a minus-end directed microtubule motor. Here, we demonstrate that the dynein light chain Tctex-1 associates with unattached kinetochores and is essential for accurate chromosome segregation. Tctex-1 knockdown in cells does not affect the localization and function of dynein at the kinetochore, but produces a prolonged mitotic arrest with a few misaligned chromosomes, which are subsequently missegregated during anaphase. This function is independent of Tctex-1's association with dynein. The kinetochore localization of Tctex-1 is independent of the ZW10-dynein pathway, but requires the Ndc80 complex. Thus, our findings reveal a dynein independent role of Tctex-1 at the kinetochore to enhance the stability of kinetochore-microtubule attachment.
a Department of Pathology and Cell Biology ; Columbia University College of Physicians and Surgeons ; New York , NY USA.
Chenshu
C
Liu
Jen-Zen
JZ
Chuang
Ching-Hwa
CH
Sung
Yinghui
Y
Mao
D002460:Cell Line; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D020090:Chromosome Segregation; D003598:Cytoskeletal Proteins; D004398:Dyneins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D018386:Kinetochores / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism*; D008938:Mitosis; D009363:Neoplasm Proteins / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D034622:RNA Interference; D015398:Signal Transduction; D013997:Time Factors; D014162:Transfection
D002868:Chromosomal Proteins, Non-Histone; D003598:Cytoskeletal Proteins; C533376:DYNLT1 protein, human; D008869:Microtubule-Associated Proteins; C120529:NDC80 protein, human; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; C488261:ZW10 protein, human; C076744:cytoplasmic linker protein 170; C557011:DYNLT3 protein, human; D004398:Dyneins
Tctex-1; dynein; kinetochore; mitosis