Expression and functional activity of neurotransmitter system components in sea urchins' early development.
Reverse-transcription polymerase chain reaction (RT-PCR) investigation of the expression of the components supposedly taking part in serotonin regulation of the early development of Paracentrotus lividus has shown the presence of transcripts of five receptors, one of which has conservative amino acid residues characteristic of monoaminergic receptors. At the early stages of embryogenesis the expressions of serotonin transporter (SERT) and noradrenaline transporter (NET) were also recognized. The activities of the enzymes of serotonin synthesis and serotonin transporter were shown using immunohistochemistry and incubation with para-chlorophenylalanine (PСРА) and 5-hydroxytryptophan (HTP). Pharmacological experiments have shown a preferential cytostatic activity of ligands characterized as mammalian 5-hydroxytryptamine (5-HT)1-antagonists. On the basis of the sum of the data from molecular biology and embryo physiological experiments, it is suggested that metabotropic serotonin receptors and membrane transporters take part in the regulatory processes of early sea urchin embryogenesis.
N.K. Koltzov Institute of Developmental Biology,Russian Academy of Sciences,Moscow,Russia.
Denis A
DA
Nikishin
Institute of Marine Biology,Kotor,Montenegro.
Ivan
I
Milošević
Institute of Marine Biology,Kotor,Montenegro.
Milorad
M
Gojković
Serbian Academy of Science and Art,Belgrade,Serbia.
Ljubisav
L
Rakić
M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bio-Organic Chemistry,Russian Academy of Sciences,Moscow,Russia.
Vladimir V
VV
Bezuglov
26,Vavilov St,Moscow 119334,Russia.
Yuri B
YB
Shmukler
D000595:Amino Acid Sequence; D000818:Animals; D047308:Arbacia / Q000196:embryology / Q000235:genetics* / Q000378:metabolism; D050483:Dopamine Plasma Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000196:embryology / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D050479:Neurotransmitter Transport Proteins / Q000235:genetics* / Q000378:metabolism; D050484:Norepinephrine Plasma Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D047328:Paracentrotus / Q000196:embryology / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D050486:Serotonin Plasma Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D050493:Vesicular Monoamine Transport Proteins / Q000235:genetics / Q000378:metabolism
D050483:Dopamine Plasma Membrane Transport Proteins; D050479:Neurotransmitter Transport Proteins; D050484:Norepinephrine Plasma Membrane Transport Proteins; D050486:Serotonin Plasma Membrane Transport Proteins; D050493:Vesicular Monoamine Transport Proteins
Embryo; Receptor; Sea urchin; Serotonin; Transporter