Analysis of the effects of dietary fat on body and skin lipids of hamsters by Raman spectroscopy.
Raman spectroscopy has previously been applied for studying lipid metabolism. In this study, a ball lens-installed hollow optical fiber Raman probe (BHRP) was used for the noninvasive measurement of skin lipids in hamsters. Our analysis suggested that multi-unsaturated lipids, once converted into a structure containing conjugated double bonds, were oxidized to form peroxides. These results were applied for analyzing lipid metabolism in adipose and skin tissues in hamsters fed tricaprin, saturated medium-chain triglyceride and trilinolein, unsaturated long-chain triglyceride fat diets. Unsaturated lipids formed conjugated structures in skin tissue but not in adipose tissue. Principal component analysis (PCA) revealed that the dietary fat intake correlated strongly with lipid composition in body and skin tissues. Hence, the present results successfully demonstrate that Raman spectroscopy with a BHRP can be a powerful tool for analyzing lipid metabolism.
Department of Biomedical Chemistry, School of Science and Technology, Kwansei Gakuin University, Gakuen, Sanda, Hyogo 669-1337, Japan. hidesato@kwansei.ac.jp.
Phiranuphon
P
Meksiarun
Yui
Y
Maeda
Tatsuya
T
Hiroi
Bibin B
BB
Andriana
Hidetoshi
H
Sato
D000818:Animals; D006224:Cricetinae; D004041:Dietary Fats / Q000494:pharmacology*; D005240:Feasibility Studies; D050356:Lipid Metabolism / Q000187:drug effects*; D015227:Lipid Peroxidation / Q000187:drug effects; D012867:Skin / Q000187:drug effects* / Q000378:metabolism*; D013059:Spectrum Analysis, Raman
D004041:Dietary Fats