Carbosulfan-induced oxidative damage following subchronic exposure and the protective effects of N-acetylcysteine in rats.
Carbosulfan (CB)-induced oxidative stress leads to the inevitable accumulation of free radicals and eventual alteration of antioxidant enzymes in various biological systems. The present study is designed to investigate the preventive effect of N-acetylcysteine (NAC) on carbosulfan-induced hepatic and renal dysfunction in rats. Rats exposed to CB and NAC were examined for toxicity by assessing various biochemical alteration and stress markers including in liver and kidney. Significant increases of blood alanine aminotransferase (ALT), alkaline phosphatase (ALP), gamma glutamyltransferase (GGT), creatinine and urea were detected in CB-treated rats. In addition, the levels of antioxidative enzymes such as catalase (CAT), superoxide dismutase (SOD) and reduced glutathione (GSH) also were assessed. According to the results, rats exposed to carbosulfan showed a significant increase in the accumulation of stress markers and an alteration in the antioxidative enzymes activity, when compared to their respective controls. Interestingly, administration of NAC to CB-treated rats attenuates the toxicity of this compound, objectified by biochemical and oxidative improvement of liver and kidney. Thus, the present study reports for the first time that NAC could be a promising therapeutic agent against CB induced oxidative stress.
Laboratory of Aggression Physiology and Endocrine Metabolic Studies, Department of Biology, Faculty of Sciences, Tunis, Tunisia. inesbini@yahoo.fr.
Ines
I
El-Bini Dhouib
Alya
A
Annabi
Aicha
A
Jrad
Narges
N
El-Golli
Najoua
N
Gharbi
Mohamed M
MM
Lasram
Saloua
S
El-Fazaa
D000111:Acetylcysteine / Q000008:administration & dosage*; D000818:Animals; D002219:Carbamates / Q000506:poisoning*; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology / Q000378:metabolism* / Q000517:prevention & control*; D004347:Drug Interactions; D004357:Drug Synergism; D007674:Kidney Diseases / Q000139:chemically induced* / Q000378:metabolism* / Q000517:prevention & control; D018384:Oxidative Stress / Q000187:drug effects; D010575:Pesticides / Q000506:poisoning; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D016896:Treatment Outcome
D002219:Carbamates; D010575:Pesticides; D017382:Reactive Oxygen Species; C035038:carbosulfan; D000111:Acetylcysteine