Action of hyperbaric oxygenation in the rat skin flap.
OBJECTIVE
To evaluate the morphology, necrotic area and collagen content in skin flaps of rats subjected to hyperbaric oxygenation (HBO).


METHODS
Forty adult rats were divided into four groups: GEC - epilated; GE/HBO - epilated subjected to HBO; GER - epilated submitted to skin flap (2 cm in width /8 cm length in the dorsal area) and GER/HBO - epilated, subjected to skin flap and HBO. HBO (2.4 ATA) was performed for two hours during seven consecutive days. In the eighth day, the rats were anesthetized and the skin flaps were removed and separated into three portions, relative to pedicle fixation. The material fixed in 10% formalin was processed for paraffin embedding; sections were stained by H.E and subjected to picrosirius-red method. The slides examined under light microscopy for evaluation of the collagen content in polarized light microscope and ImageLab(r) software (Bio-Rad).


RESULTS
The data showed larger area of necrosis and lower levels of collagen in the three regions of the GER group, whereas in the GER/HBO group the collagen content was similar to the GEC and GE/HBO groups.


CONCLUSIONS
Hyperbaric oxygenation reduced the area of necrosis and preserved the morphology and collagen content in skin flaps of rats.
EPM, UNIFESP, Brazil.
Fabricio Valandro
FV
Rech
UNIFESP, Sao Paulo, SP, Brazil.
Anna Luiza Negrini
AL
Fagundes
UNIFESP, Sao Paulo, SP, Brazil.
Ricardo Santos
RS
Simões
UNIFESP, Sao Paulo, SP, Brazil.
Rinaldo
R
Florencio-Silva
UNIFESP, Sao Paulo, SP, Brazil.
Gisela Rodrigues da Silva
GR
Sasso
UNIFESP, Sao Paulo, SP, Brazil.
Murched Omar
MO
Taha
UNIFESP, Sao Paulo, SP, Brazil.
Djalma José
DJ
Fagundes
D000818:Animals; D001706:Biopsy; D003094:Collagen / Q000032:analysis*; D006931:Hyperbaric Oxygenation / Q000379:methods*; D009336:Necrosis / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D015203:Reproducibility of Results; D012867:Skin / Q000473:pathology*; D016038:Skin Transplantation / Q000379:methods*; D013524:Surgical Flaps / Q000473:pathology* / Q000502:physiology*; D013997:Time Factors; D016896:Treatment Outcome; D014945:Wound Healing
D003094:Collagen