Graphene oxide-mediated electrochemistry of glucose oxidase on glassy carbon electrodes.
Glucose oxidase (GOD) was immobilized on glassy carbon electrodes in the presence of graphene oxide (GO) as a model system for the interaction between GO and biological molecules. Lyotropic properties of didodecyldimethylammonium bromide (DDAB) were used to stabilize the enzymatic layer on the electrode surface resulting in a markedly improved electrochemical response of the immobilized GOD. Transmission electron microscopy images of the GO with DDAB confirmed the distribution of the GO in a two-dimensional manner as a foil-like material. Although it is known that glassy carbon surfaces are not ideal for hydrogen peroxide detection, successful chronoamperometric titrations of the GOD in the presence of GO with β-d-glucose were performed on glassy carbon electrodes, whereas no current response was detected upon β-d-glucose addition in the absence of GO. The GOD-DDAB-GO system displayed a high turnover efficiency and substrate affinity as a glucose biosensor. The simplicity and ease of the electrode preparation procedure of this GO/DDAB system make it a good candidate for immobilizing other biomolecules for fabrication of amperometric biosensors.
Department of Life Sciences and Systems Biology, University of Torino, Torino, Italy.
Silvia
S
Castrignanò
Department of Life Sciences and Systems Biology, University of Torino, Torino, Italy.
Francesca
F
Valetti
Department of Life Sciences and Systems Biology, University of Torino, Torino, Italy.|Centre for Nanostructured Interfaces and Surfaces, University of Torino, Torino, Italy.
Gianfranco
G
Gilardi
Department of Life Sciences and Systems Biology, University of Torino, Torino, Italy.|Centre for Nanostructured Interfaces and Surfaces, University of Torino, Torino, Italy.
Sheila J
SJ
Sadeghi
D002244:Carbon / Q000737:chemistry*; D055664:Electrochemical Techniques; D004566:Electrodes; D005947:Glucose / Q000032:analysis; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism; D006108:Graphite / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D010087:Oxides / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry / Q000378:metabolism; D013499:Surface Properties
D010087:Oxides; D000644:Quaternary Ammonium Compounds; C046112:didodecyldimethylammonium; D002244:Carbon; D006108:Graphite; D005949:Glucose Oxidase; D005947:Glucose
glassy carbon; glucose biosensor; glucose oxidase; graphene oxide