The yeast two-hybrid system: a tool for mapping protein-protein interactions.
Virtually all processes in living cells are dependent on protein-protein interactions (PPIs). Understanding PPI networks is thus essential for molecular biology and disease research. One powerful genetic system for mapping PPIs both at a small scale and in a high-throughput manner is the yeast two-hybrid (Y2H) screen. In Y2H screening, PPIs are detected through the activation of reporter genes responding to a reconstituted transcription factor. In this introduction, we describe library- and array-based Y2H methods and explain their basic theory. We also include the rationale behind different Y2H approaches and strategies for optimizing results.
Center for the Study of Biological Complexity, Virginia Commonwealth University, Richmond, Virginia 23284.
Jitender
J
Mehla
Center for the Study of Biological Complexity, Virginia Commonwealth University, Richmond, Virginia 23284.
J Harry
JH
Caufield
Center for the Study of Biological Complexity, Virginia Commonwealth University, Richmond, Virginia 23284.
Peter
P
Uetz
D060066:Protein Interaction Maps; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D020798:Two-Hybrid System Techniques
D011994:Recombinant Proteins