miR-330-3p controls cell proliferation by targeting early growth response 2 in non-small-cell lung cancer.
Non-small-cell lung cancer (NSCLC) is one of the most common lung cancers, and microRNAs (miRNAs) have been reported to play essential roles in NSCLC. Recent studies have indicated that miR-330-3p expression is up-regulated in NSCLC samples and in tissues of NSCLC brain metastasis. In this study, up-regulation of miR-330-3p expression was confirmed in NSCLC and 20 NSCLC patient samples. Furthermore, miR-330-3p was over-expressed in NSCLC cell lines A549 and H23, and the promotive function of miR-330-3p was investigated in regulating NSCLC cell proliferation and cell cycle distribution. To identify potential target genes of miR-330-3p in NSCLC, the miRNA target prediction databases were used. Luciferase activity assay and real-time RT-PCR analysis confirmed that miR-330-3p is negatively correlated with the expression of early growth response 2 (EGR2). Moreover, it was also found that EGR2 mRNA contains two potential binding sites for miR-330-3p. Knock-down of EGR2 with siRNA was demonstrated to have a similar effect as the over-expression of miR-330-3p in NSCLC cell lines. Taken together, our results show that EGR2 is a target of miR-330-3p.
Department of Respiratory Medicine, the Third Affiliated Hospital of Qiqihar Medical University, Qiqihar 161000, China.
Xuzhi
X
Liu
Department of Respiratory Medicine, the Third Affiliated Hospital of Qiqihar Medical University, Qiqihar 161000, China.
Hanbing
H
Shi
Department of Respiratory Medicine, the Third Affiliated Hospital of Qiqihar Medical University, Qiqihar 161000, China.
Bo
B
Liu
Department of Respiratory Medicine, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China.
Jianing
J
Li
Department of Respiratory Medicine, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China.
Yaxin
Y
Liu
Department of Respiratory Medicine, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China yubaiquan76@163.com.
Baiquan
B
Yu
D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000502:physiology*; D051767:Early Growth Response Protein 2 / Q000378:metabolism*; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D035683:MicroRNAs / Q000378:metabolism / Q000502:physiology*; D015854:Up-Regulation
C497326:EGR2 protein, human; D051767:Early Growth Response Protein 2; C570805:MIRN330 microRNA, human; D035683:MicroRNAs
EGR2; miR-330-3p; non-small-cell lung cancer; proliferation