Catheter-based intramural delivery of red blood cells in an animal model of atherosclerosis.
This report demonstrates intramural red blood cell (RBC) delivery in an atherosclerotic rabbit aorta model and validates the ability of fluoroscopy and computed tomography to verify RBC deposition. A microinfusion catheter with a 35-gauge needle delivered RBCs mixed with iodinated contrast agent to the aorta wall. Six rabbits were sacrificed after injection to confirm RBC delivery. Iron deposition was examined in four additional rabbits 3-7 weeks after injection. Imaging demonstrated 86% sensitivity and 100% specificity for the detection of RBC deposition (n = 25 injection attempts). Iron deposits were found in all intraplaque injection sites 3-7 weeks after injection.
Sunnybrook Research Institute, Department of Medical Imaging, University of Toronto, 2075 Bayview Avenue, Toronto, Ontario, Canada M4N 3M5. Electronic address: stephanieelaine.chiu@utoronto.ca.
Stephanie E G
SE
Chiu
Sunnybrook Health Sciences Centre, and Department of Medical Imaging, 2075 Bayview Avenue, Toronto, Ontario, Canada M4N 3M5.
James Q
JQ
Zhan
Sunnybrook Research Institute, Department of Medical Imaging, University of Toronto, 2075 Bayview Avenue, Toronto, Ontario, Canada M4N 3M5; Sunnybrook Health Sciences Centre, and Department of Medical Imaging, 2075 Bayview Avenue, Toronto, Ontario, Canada M4N 3M5; Department of Medical Biophysics, University of Toronto, 2075 Bayview Avenue, Toronto, Ontario, Canada M4N 3M5.
Alan R
AR
Moody
D000818:Animals; D001011:Aorta / Q000473:pathology; D050197:Atherosclerosis / Q000503:physiopathology*; D002404:Catheterization; D004195:Disease Models, Animal; D004912:Erythrocytes; D007501:Iron / Q000378:metabolism; D008297:Male; D011817:Rabbits
D007501:Iron