Measuring ribonucleotide incorporation into DNA in vitro and in vivo.
Ribonucleotides are incorporated into genomes by DNA polymerases, they can be removed, and if not removed, they can have deleterious and beneficial consequences. Here, we describe an assay to quantify stable ribonucleotide incorporation by DNA polymerases in vitro, and an assay to probe for ribonucleotides in each of the two DNA strands of the yeast nuclear genome.
Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC, 27709, USA.
Anders R
AR
Clausen
Jessica S
JS
Williams
Thomas A
TA
Kunkel
D000468:Alkalies / Q000494:pharmacology; D001483:Base Sequence; D001681:Biological Assay / Q000379:methods*; D004271:DNA, Fungal / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D016681:Genome, Fungal; D008969:Molecular Sequence Data; D012265:Ribonucleotides / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013194:Staining and Labeling
D000468:Alkalies; D004271:DNA, Fungal; D012265:Ribonucleotides