Swimming training prevents alterations in ecto-NTPDase and adenosine deaminase activities in lymphocytes from Nω-nitro-L-arginine methyl ester hydrochloride induced hypertension rats.
METHODS
Hypertension is accompanied by inflammatory process and purinergic system has been recognized as having an important role in modulating immune functions. Physical training is being considered one of the major lifestyle changes that contributes to the cardiovascular health as well as has an important role in regulating purinergic system. Thus, the aim of this study was to investigate the effect of chronic swimming training on lymphocytic purinergic system enzymes activities related to inflammatory process, as well as in lipid profile and classic inflammatory markers in rats that developed hypertension in response to the oral administration of N-nitro-L-arginine methyl ester hydrochloride (L-NAME).


RESULTS
After 6 weeks of training, lymphocytes and serum were separated to be analysed. L-NAME-treated group displayed an increase in SBP as well as in ecto-NTPDase and adenosine deaminase (ADA) activities (P < 0.05). Six weeks of swimming training were able to prevent these alterations and keep the blood pressure and enzymes activities in the same levels of control group. Exercise per se was associated with a decrease in the expression of ecto-NTPDase1 in lymphocytes (-23.4%). Exercise was also efficient in preventing the rise in classic inflammatory markers observed in L-NAME group.


CONCLUSIONS
These findings highlight the link between purinergic signalling and inflammatory process and suggest a novel mechanism in which moderate aerobic exercise possesses the potential to attenuate inflammation caused by hypertension.
aPost-Graduation Program in Toxicological Biochemistry, Center of Natural and Exact Sciences of the Federal University of Santa Maria, Santa Maria bCollegiate of Nursing, University of Southern Frontier, Chapecó Campus cInstituto Federal de Educação, Ciência e Tecnologia do Triângulo Mineiro, CEP: 38.600-000, Câmpus- Paracatu-MG dPost-Graduation Program in Pharmacy, Center of Biological and Health Sciences of the Federal University of Mato Grosso do Sul, Campo Grande, Brazil.
Andréia Machado
AM
Cardoso
Fátima Husein
FH
Abdalla
Margarete Dulce
MD
Bagatini
Caroline Curry
CC
Martins
Daniela
D
Zanini
Roberta
R
Schmatz
Jeandre Augusto
JA
Jaques
Daniela Bitencourt Rosa
DB
Leal
Vera Maria
VM
Morsch
Maria Rosa Chitolina
MR
Schetinger
D000243:Adenosine Deaminase / Q000378:metabolism*; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D001081:Apyrase / Q000378:metabolism*; D001794:Blood Pressure; D006973:Hypertension / Q000139:chemically induced / Q000276:immunology / Q000628:therapy*; D008214:Lymphocytes / Q000201:enzymology*; D008297:Male; D019331:NG-Nitroarginine Methyl Ester; D010805:Physical Conditioning, Animal; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013550:Swimming / Q000502:physiology
D015703:Antigens, CD; D000243:Adenosine Deaminase; D001081:Apyrase; C067869:CD39 antigen; D019331:NG-Nitroarginine Methyl Ester