Innate and adaptive immune functions of peyer's patch monocyte-derived cells.
Peyer's patches (PPs) are primary inductive sites of mucosal immunity. Defining PP mononuclear phagocyte system (MPS) is thus crucial to understand the initiation of mucosal immune response. We provide a comprehensive analysis of the phenotype, distribution, ontogeny, lifespan, function, and transcriptional profile of PP MPS. We show that monocytes give rise to macrophages and to lysozyme-expressing dendritic cells (LysoDCs), which are both involved in particulate antigen uptake, display strong innate antiviral and antibacterial gene signatures, and, upon TLR7 stimulation, secrete IL-6 and TNF, but neither IL-10 nor IFNγ. However, unlike macrophages, LysoDCs display a rapid renewal rate, strongly express genes of the MHCII presentation pathway, and prime naive helper T cells for IFNγ production. Our results show that monocytes differentiate locally into LysoDCs and macrophages, which display distinct features from their adjacent villus counterparts.
Centre d'Immunologie de Marseille-Luminy (CIML), Aix-Marseille University, UM2, 13288 Marseille, France; Institut National de la Santé et de la Recherche Médicale (INSERM), U1104, 13288 Marseille, France; Centre National de la Recherche Scientifique (CNRS), UMR7280, 13288 Marseille, France.
Johnny
J
Bonnardel
Centre d'Immunologie de Marseille-Luminy (CIML), Aix-Marseille University, UM2, 13288 Marseille, France; Institut National de la Santé et de la Recherche Médicale (INSERM), U1104, 13288 Marseille, France; Centre National de la Recherche Scientifique (CNRS), UMR7280, 13288 Marseille, France.
Clément
C
Da Silva
Centre d'Immunologie de Marseille-Luminy (CIML), Aix-Marseille University, UM2, 13288 Marseille, France; Institut National de la Santé et de la Recherche Médicale (INSERM), U1104, 13288 Marseille, France; Centre National de la Recherche Scientifique (CNRS), UMR7280, 13288 Marseille, France.
Sandrine
S
Henri
Centre d'Immunologie de Marseille-Luminy (CIML), Aix-Marseille University, UM2, 13288 Marseille, France; Institut National de la Santé et de la Recherche Médicale (INSERM), U1104, 13288 Marseille, France; Centre National de la Recherche Scientifique (CNRS), UMR7280, 13288 Marseille, France.
Samira
S
Tamoutounour
Centre d'Immunologie de Marseille-Luminy (CIML), Aix-Marseille University, UM2, 13288 Marseille, France; Institut National de la Santé et de la Recherche Médicale (INSERM), U1104, 13288 Marseille, France; Centre National de la Recherche Scientifique (CNRS), UMR7280, 13288 Marseille, France.
Lionel
L
Chasson
Centre d'Immunologie de Marseille-Luminy (CIML), Aix-Marseille University, UM2, 13288 Marseille, France; Institut National de la Santé et de la Recherche Médicale (INSERM), U1104, 13288 Marseille, France; Centre National de la Recherche Scientifique (CNRS), UMR7280, 13288 Marseille, France.
Frederic
F
Montañana-Sanchis
Centre d'Immunologie de Marseille-Luminy (CIML), Aix-Marseille University, UM2, 13288 Marseille, France; Institut National de la Santé et de la Recherche Médicale (INSERM), U1104, 13288 Marseille, France; Centre National de la Recherche Scientifique (CNRS), UMR7280, 13288 Marseille, France.
Jean-Pierre
JP
Gorvel
Centre d'Immunologie de Marseille-Luminy (CIML), Aix-Marseille University, UM2, 13288 Marseille, France; Institut National de la Santé et de la Recherche Médicale (INSERM), U1104, 13288 Marseille, France; Centre National de la Recherche Scientifique (CNRS), UMR7280, 13288 Marseille, France. Electronic address: lelouard@ciml.univ-mrs.fr.
Hugues
H
Lelouard
D056704:Adaptive Immunity; D000818:Animals; D002454:Cell Differentiation; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D007113:Immunity, Innate; D007371:Interferon-gamma / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008264:Macrophages / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal; D009000:Monocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D010581:Peyer's Patches / Q000166:cytology*; D010641:Phenotype; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology / Q000378:metabolism; D051199:Toll-Like Receptor 7 / Q000378:metabolism; D059467:Transcriptome; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D015850:Interleukin-6; D008562:Membrane Glycoproteins; C477038:Tlr7 protein, mouse; D051199:Toll-Like Receptor 7; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma