Improving biodiversity assessment of anuran amphibians using DNA barcoding of tadpoles. Case studies from Southeast Asia.
Amphibian populations are dramatically declining, while their inventory is far from being achieved. Tadpoles are usually overlooked from biodiversity survey, whereas their consideration will optimize species counts and knowledge of their ecological and developmental requirements is essential in conservation planning. Two mitochondrial markers, 16S (397 new sequences obtained) and COI (343 new sequences obtained), are used to test DNA barcoding on a set of larval and adult Asian amphibians represented by 83 recognized species from 65 sites. The advantages and drawbacks of each marker are assessed, COI barcoding being advocated for global DNA barcoding, whereas 16S suits for taxonomically or geographically restricted DNA barcoding. About half of the collected tadpoles were badly identified or incompletely named in the field. All tadpole sequences (except one case of probable introgressive hybridization) were correctly assigned to their respective species. Finally six clusters of tadpole sequences without conspecific adults were revealed, stressing the importance of collecting and taking into account tadpoles in biodiversity survey and conservation planning.
Institut de systématique, évolution, biodiversité, ISYEB-UMR 7205-CNRS, MNHN, UPMC, EPHE, Muséum national d'histoire naturelle, Sorbonne universités, 57, rue Cuvier, CP 30, 75005 Paris, France. Electronic address: stephane.grosjean@mnhn.fr.
Stéphane
S
Grosjean
Institut de systématique, évolution, biodiversité, ISYEB-UMR 7205-CNRS, MNHN, UPMC, EPHE, Muséum national d'histoire naturelle, Sorbonne universités, 57, rue Cuvier, CP 30, 75005 Paris, France.
Annemarie
A
Ohler
Department of Biology, Faculty of Science, Khon Kaen University, Nai Muaeng, Mueang, 40002 Khon Kaen, Thailand.
Yodchaiy
Y
Chuaynkern
Genoscope, Centre national de séquençage, 2, rue Gaston-Crémieux, CP 5706, 91057 Évry cedex, France.
Corinne
C
Cruaud
Institut de systématique, évolution, biodiversité, ISYEB-UMR 7205-CNRS, MNHN, UPMC, EPHE, Muséum national d'histoire naturelle, Sorbonne universités, 57, rue Cuvier, CP 51, 75005 Paris, France; Sorbonne universités, université Paris-6 (UPMC), UFR927, 4, place Jussieu, 75005 Paris, France.
Alexandre
A
Hassanin
D000818:Animals; D001001:Anura / Q000145:classification / Q000502:physiology*; D001210:Asia, Southeastern; D044822:Biodiversity; D002965:Classification; D003247:Conservation of Natural Resources; D004247:DNA / Q000235:genetics*; D058893:DNA Barcoding, Taxonomic; D004784:Environmental Monitoring / Q000379:methods*; D005819:Genetic Markers; D007814:Larva / Q000502:physiology*; D008928:Mitochondria / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D013045:Species Specificity
D005819:Genetic Markers; D004247:DNA
16S; 16S gene; Amphibian conservation; Barcoding ADN larvaire; Biodiversity survey; COI; COI gene; Conservation des amphibiens; Herpétofaune de l’Asie du Sud-Est; Larval DNA barcoding; Southeast Asian herpetofauna; Étude de biodiversité