[Characterization and comparison of interferon reference standards using UPLC-MS].
The study aims to characterize and compare interferon reference standards from 5 manufacturers. By testing molecular mass and trypsin-digested peptide mass mapping, the amino acid sequence was verified and post-translational modifications such as disulfide bond were identified. Results show that the molecular mass and amino acid sequence were consistent with theory; the disulfide bonds of 4 lots of interferon were Cys1-Cys98/Cys29-Cys138, 1 lot was Cys29-Cys139/Cys86-Cys99; N-terminal "+Met", acetyl N-terminal and Met oxidation were identified in part of the sample. UPLC-MS can be used to characterize and compare interferon reference standards from different manufacturers.
Lei
L
Tao
De-ning
DN
Pei
Chun-mei
CM
Han
Wei
W
Chen
Chun-ming
CM
Rao
Jun-zhi
JZ
Wang
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007372:Interferons / Q000592:standards*; D013058:Mass Spectrometry / Q000379:methods*; D008970:Molecular Weight; D010084:Oxidation-Reduction; D010449:Peptide Mapping; D011499:Protein Processing, Post-Translational; D012015:Reference Standards
D007372:Interferons