A novel ultrasensitive phosphate amperometric nanobiosensor based on the integration of pyruvate oxidase with highly ordered gold nanowires array.
A novel phosphate amperometric nanobiosensor, based on an intimate integration of pyruvate oxidase (PyOx) and its cofactors, thiamine pyrophosphate (TPP) and flavin adenine dinucleotide (FAD), with a highly ordered gold nanowires array (AuNWA) has been developed. The successful integration of PyOx and the co-factors, via crosslinking with bovine serum albumin (BSA) and glutaraldehyde (GLA), onto the AuNWA was confirmed by cyclic voltammetry and amperometry. The resulting nanobiosensor achieved a detection limit of 0.1 µM, a linear concentration range of 12.5-1000 µM, and a sensitivity of 140.3 µA mM(-1)cm(-2). Notably, the incorporation of the AuNWA reduced the required PyOx concentration by 70-120 fold and the presence of common interferants, such as chloride, sulfate, fluoride, nitrite and nitrate ions did not interfere with phosphate detection. Furthermore, the nanobiosensor demonstrated a very high stability with repeated use over two weeks and was successfully used for the determination of phosphate in water samples with an average recovery of 96.6 ± 4.9%.
NanoScience and Sensor Technology Research Group, School of Chemistry, Monash University, Clayton, Victoria 3800, Australia.
Edward
E
Ogabiela
NanoScience and Sensor Technology Research Group, School of Chemistry, Monash University, Clayton, Victoria 3800, Australia. Electronic address: Sam.Adeloju@monash.edu.
Samuel B
SB
Adeloju
Laboratory for Functional Nanomaterials and Devices, School of Materials Science and Engineering, Hefei University of Technology, Hefei 230009, Anhui, China.
Jiewu
J
Cui
Laboratory for Functional Nanomaterials and Devices, School of Materials Science and Engineering, Hefei University of Technology, Hefei 230009, Anhui, China.
Yucheng
Y
Wu
School of Biotechnology & Food Engineering, Hefei University of Technology, Hefei 230009, Anhui, China.
Wei
W
Chen
D015374:Biosensing Techniques / Q000295:instrumentation; D003217:Conductometry / Q000295:instrumentation*; D004566:Electrodes; D004784:Environmental Monitoring / Q000295:instrumentation; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D008968:Molecular Conformation; D036103:Nanotechnology / Q000295:instrumentation; D053770:Nanowires / Q000737:chemistry* / Q000648:ultrastructure; D010710:Phosphates / Q000032:analysis* / Q000737:chemistry; D011771:Pyruvate Oxidase / Q000737:chemistry* / Q000648:ultrastructure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D018511:Systems Integration; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000737:chemistry
D004800:Enzymes, Immobilized; D010710:Phosphates; D014874:Water Pollutants, Chemical; D006046:Gold; D011771:Pyruvate Oxidase
Amperometry; Crosslinking; Gold nanowires array; Nanobiosensor; Phosphate; Pyruvate oxidase