Characterising Non-Structural Protein NS4 of African Horse Sickness Virus.
African horse sickness is a serious equid disease caused by the orbivirus African horse sickness virus (AHSV). The virus has ten double-stranded RNA genome segments encoding seven structural and three non-structural proteins. Recently, an additional protein was predicted to be encoded by genome segment 9 (Seg-9), which also encodes VP6, of most orbiviruses. This has since been confirmed in bluetongue virus and Great Island virus, and the non-structural protein was named NS4. In this study, in silico analysis of AHSV Seg-9 sequences revealed the existence of two main types of AHSV NS4, designated NS4-I and NS4-II, with different lengths and amino acid sequences. The AHSV NS4 coding sequences were in the +1 reading frame relative to that of VP6. Both types of AHSV NS4 were expressed in cultured mammalian cells, with sizes close to the predicted 17-20 kDa. Fluorescence microscopy of these cells revealed a dual cytoplasmic and nuclear, but not nucleolar, distribution that was very similar for NS4-I and NS4-II. Immunohistochemistry on heart, spleen, and lung tissues from AHSV-infected horses showed that NS4 occurs in microvascular endothelial cells and mononuclear phagocytes in all of these tissues, localising to the both the cytoplasm and the nucleus. Interestingly, NS4 was also detected in stellate-shaped dendritic macrophage-like cells with long cytoplasmic processes in the red pulp of the spleen. Finally, nucleic acid protection assays using bacterially expressed recombinant AHSV NS4 showed that both types of AHSV NS4 bind dsDNA, but not dsRNA. Further studies will be required to determine the exact function of AHSV NS4 during viral replication.
Department of Genetics, University of Pretoria, Pretoria, South Africa.
Lizahn
L
Zwart
Deltamune (Pty) Ltd, Lyttelton, Centurion, South Africa; Department of Biochemistry, Centre for Human Metabonomics, North-West University, Potchefstroom, South Africa.
Christiaan A
CA
Potgieter
Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.
Sarah J
SJ
Clift
Department of Genetics, University of Pretoria, Pretoria, South Africa.
Vida
V
van Staden
D000355:African Horse Sickness / Q000473:pathology / Q000821:virology; D000356:African Horse Sickness Virus / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D016679:Genome, Viral; D005838:Genotype; D006736:Horses; D042541:Intracellular Space / Q000378:metabolism; D016366:Open Reading Frames; D010802:Phylogeny; D021381:Protein Transport; D017422:Sequence Analysis, DNA; D065288:Serogroup; D017361:Viral Nonstructural Proteins / Q000235:genetics* / Q000378:metabolism*
D004268:DNA-Binding Proteins; D017361:Viral Nonstructural Proteins