Bactericidal Mechanism of Bio-oil Obtained from Fast Pyrolysis of Pinus densiflora Against Two Foodborne Pathogens, Bacillus cereus and Listeria monocytogenes.
Foodborne bacteria are the leading cause of food spoilage and other related diseases. In the present study, the antibacterial activity of bio-oil (BO) manufactured by fast pyrolysis of pinewood sawdust (Pinus densiflora Siebold and Zucc.) against two disease-causing foodborne pathogens (Bacillus cereus and Listeria monocytogenes) was evaluated. BO at a concentration of 1000 μg/disc was highly active against both B. cereus (10.0-10.6 mm-inhibition zone) and L. monocytogenes (10.6-12.0-mm inhibition zone). The minimum inhibitory concentration (MIC) and minimum bactericidal concentration values of BO were 500 and 1000 μg/mL, respectively, for both pathogens. At the MIC concentration, BO exhibited an inhibitory effect on the viability of the bacterial pathogens. The mechanism of action of BO revealed its strong impairing effect on the membrane integrity of bacterial cells, which was confirmed by a marked release of 260-nm absorbing material, leakage of electrolytes and K(+) ions, and reduced capacity for osmoregulation under high salt concentration. Scanning electron microscopy clearly showed morphological alteration of the cell membrane due to the effect of BO. Overall, the results of this study suggest that BO exerts effective antibacterial potential against foodborne pathogens and can therefore potentially be used in food processing and preservation.
1 School of Biotechnology, Yeungnam University , Gyeongsan, Gyeongbuk, Korea.
Jayanta Kumar
JK
Patra
2 Department of Forest Sciences, Seoul National University , Seoul, Korea.
Hyewon
H
Hwang
3 Graduate School of International Agricultural Technology and Institute of Green-Bio Science and Technology, Seoul National University , Pyeongchang, Korea.
Joon Weon
JW
Choi
1 School of Biotechnology, Yeungnam University , Gyeongsan, Gyeongbuk, Korea.
Kwang-Hyun
KH
Baek
D000900:Anti-Bacterial Agents / Q000191:economics / Q000302:isolation & purification / Q000494:pharmacology*; D001409:Bacillus cereus / Q000187:drug effects* / Q000254:growth & development / Q000472:pathogenicity / Q000648:ultrastructure; D056804:Biofuels / Q000191:economics; D002463:Cell Membrane Permeability / Q000187:drug effects; D060389:Construction Industry / Q000191:economics; D005517:Foodborne Diseases / Q000382:microbiology / Q000517:prevention & control; D006358:Hot Temperature; D006801:Humans; D007220:Industrial Waste / Q000032:analysis / Q000191:economics; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000254:growth & development / Q000472:pathogenicity / Q000648:ultrastructure; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability; D008855:Microscopy, Electron, Scanning; D064587:Osmoregulation / Q000187:drug effects; D028223:Pinus / Q000737:chemistry*; D010938:Plant Oils / Q000191:economics / Q000302:isolation & purification / Q000494:pharmacology*; D056910:Republic of Korea; D014934:Wood / Q000737:chemistry* / Q000191:economics
D000900:Anti-Bacterial Agents; D056804:Biofuels; D007220:Industrial Waste; D010938:Plant Oils