Fabrication of inorganic hydroxyapatite nanoparticles and organic biomolecules-dual encapsulated alginate microspheres.
Inorganic hydroxyapatite nanoparticles (HANPs) and two kinds of organic biomolecules (i.e., fluorescent dye rhodamine 6G and protein lysozyme) were coencapsulated into alginate microspheres through an air dynamical atomization with optimized operation conditions. The synthesized microspheres have several advantages: HANP provides osteoconductivity and mechanical strength, rhodamine 6G (R6G) and lysozyme act as model drugs, and alginate provides excellent biocompatibility and carboxylate functionality. The results of fluorescent microscopic images indicated the successful dual encapsulation of HANPs and lysozyme inside the alginate microspheres. Furthermore, the results of 3- (4,5-cimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay showed that the fabricated alginate microspheres could be uptaken by HepG2 without apparent cytotoxicity. The dual encapsulated alginate microspheres fabricated in this study show great potential in many biomedical applications.
Department of Chemical Engineering, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, Taiwan.
Yu-Pu
YP
Wang
Department of Chemical Engineering, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, Taiwan.
Yu-Te
YT
Liao
Department of Urology, Taipei Medical University-Shuang Ho Hospital, No. 291, Jhongjheng Rd., Jhonghe Dist., New Taipei 23561, Taiwan.
Chia-Hung
CH
Liu
Department of Chemical Engineering, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, Taiwan.
Jiashing
J
Yu
Division of Medical Engineering Research, National Health Research Institutes, 35 Keyan Road, Zhunan, Miaoli County 350, Taiwan.
Jung-Chih
JC
Chen
Department of Chemical Engineering, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, Taiwan and Division of Medical Engineering Research, National Health Research Institutes, 35 Keyan Road, Zhunan, Miaoli County 350, Taiwan.
Kevin C-W
KC
Wu
D000464:Alginates; D002470:Cell Survival / Q000187:drug effects; D017886:Durapatite; D004705:Endocytosis; D020723:Glucuronic Acid; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000378:metabolism*; D006603:Hexuronic Acids; D006801:Humans; D008863:Microspheres; D053758:Nanoparticles / Q000737:chemistry* / Q000378:metabolism* / Q000633:toxicity; D036103:Nanotechnology / Q000379:methods*
D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D017886:Durapatite