Effect of cardiac arrest on cognitive impairment and hippocampal plasticity in middle-aged rats.
Cardiopulmonary arrest is a leading cause of death and disability in the United States that usually occurs in the aged population. Cardiac arrest (CA) induces global ischemia, disrupting global cerebral circulation that results in ischemic brain injury and leads to cognitive impairments in survivors. Ischemia-induced neuronal damage in the hippocampus following CA can result in the impairment of cognitive function including spatial memory. In the present study, we used a model of asphyxial CA (ACA) in nine month old male Fischer 344 rats to investigate cognitive and synaptic deficits following mild global cerebral ischemia. These experiments were performed with the goals of 1) establishing a model of CA in nine month old middle-aged rats; and 2) to test the hypothesis that learning and memory deficits develop following mild global cerebral ischemia in middle-aged rats. To test this hypothesis, spatial memory assays (Barnes circular platform maze and contextual fear conditioning) and field recordings (long-term potentiation and paired-pulse facilitation) were performed. We show that following ACA in nine month old middle-aged rats, there is significant impairment in spatial memory formation, paired-pulse facilitation n dysfunction, and a reduction in the number of non-compromised hippocampal Cornu Ammonis 1 and subiculum neurons. In conclusion, nine month old animals undergoing cardiac arrest have impaired survival, deficits in spatial memory formation, and synaptic dysfunction.
Cerebral Vascular Disease Research Laboratories, University of Miami Leonard M. Miller School of Medicine, Miami, Florida, United States of America; Evelyn F. McKnight Brain Institute, University of Miami Leonard M. Miller School of Medicine, Miami, Florida, United States of America; Department of Neurology, University of Miami Leonard M. Miller School of Medicine, Miami, Florida, United States of America; Neuroscience Program, University of Miami Leonard M. Miller School of Medicine, Miami, Florida, United States of America.
Charles H
CH
Cohan
Cerebral Vascular Disease Research Laboratories, University of Miami Leonard M. Miller School of Medicine, Miami, Florida, United States of America; Evelyn F. McKnight Brain Institute, University of Miami Leonard M. Miller School of Medicine, Miami, Florida, United States of America; Department of Neurology, University of Miami Leonard M. Miller School of Medicine, Miami, Florida, United States of America.
Jake T
JT
Neumann
Cerebral Vascular Disease Research Laboratories, University of Miami Leonard M. Miller School of Medicine, Miami, Florida, United States of America; Evelyn F. McKnight Brain Institute, University of Miami Leonard M. Miller School of Medicine, Miami, Florida, United States of America; Department of Neurology, University of Miami Leonard M. Miller School of Medicine, Miami, Florida, United States of America; Neuroscience Program, University of Miami Leonard M. Miller School of Medicine, Miami, Florida, United States of America.
Kunjan R
KR
Dave
Cerebral Vascular Disease Research Laboratories, University of Miami Leonard M. Miller School of Medicine, Miami, Florida, United States of America; Evelyn F. McKnight Brain Institute, University of Miami Leonard M. Miller School of Medicine, Miami, Florida, United States of America; Department of Neurology, University of Miami Leonard M. Miller School of Medicine, Miami, Florida, United States of America.
Aleksey
A
Alekseyenko
Cerebral Vascular Disease Research Laboratories, University of Miami Leonard M. Miller School of Medicine, Miami, Florida, United States of America; Department of Neurology, University of Miami Leonard M. Miller School of Medicine, Miami, Florida, United States of America.
Marc
M
Binkert
Cerebral Vascular Disease Research Laboratories, University of Miami Leonard M. Miller School of Medicine, Miami, Florida, United States of America; Department of Neurology, University of Miami Leonard M. Miller School of Medicine, Miami, Florida, United States of America.
Kenneth
K
Stransky
Cerebral Vascular Disease Research Laboratories, University of Miami Leonard M. Miller School of Medicine, Miami, Florida, United States of America; Evelyn F. McKnight Brain Institute, University of Miami Leonard M. Miller School of Medicine, Miami, Florida, United States of America; Department of Neurology, University of Miami Leonard M. Miller School of Medicine, Miami, Florida, United States of America.
Hung Wen
HW
Lin
Evelyn F. McKnight Brain Institute; ARL Division of Neural Systems, Memory & Aging; Departments of Psychology, Neurology and Neuroscience, University of Arizona, Tucson, United States of America.
Carol A
CA
Barnes
Evelyn F. McKnight Brain Institute, University of Miami Leonard M. Miller School of Medicine, Miami, Florida, United States of America; Department of Neurology, University of Miami Leonard M. Miller School of Medicine, Miami, Florida, United States of America; Neuroscience Program, University of Miami Leonard M. Miller School of Medicine, Miami, Florida, United States of America.
Clinton B
CB
Wright
Cerebral Vascular Disease Research Laboratories, University of Miami Leonard M. Miller School of Medicine, Miami, Florida, United States of America; Evelyn F. McKnight Brain Institute, University of Miami Leonard M. Miller School of Medicine, Miami, Florida, United States of America; Department of Neurology, University of Miami Leonard M. Miller School of Medicine, Miami, Florida, United States of America; Neuroscience Program, University of Miami Leonard M. Miller School of Medicine, Miami, Florida, United States of America.
Miguel A
MA
Perez-Pinzon
D000375:Aging; D000818:Animals; D001237:Asphyxia / Q000150:complications / Q000503:physiopathology; D056547:CA1 Region, Hippocampal / Q000473:pathology / Q000503:physiopathology; D003072:Cognition Disorders / Q000150:complications / Q000473:pathology / Q000503:physiopathology*; D003213:Conditioning, Psychological; D019706:Excitatory Postsynaptic Potentials; D005239:Fear; D046352:Freezing Reaction, Cataleptic; D006323:Heart Arrest / Q000150:complications / Q000503:physiopathology*; D006624:Hippocampus / Q000473:pathology / Q000503:physiopathology*; D008297:Male; D018782:Maze Learning; D008569:Memory Disorders / Q000473:pathology / Q000503:physiopathology; D009473:Neuronal Plasticity; D011916:Rats, Inbred F344; D016019:Survival Analysis; D009435:Synaptic Transmission