Quercetin induces the apoptosis of human ovarian carcinoma cells by upregulating the expression of microRNA-145.
Ovarian cancer is one of the most malignant types of cancer of the female human reproductive track, posing a severe threat to the health of the female population. Numerous previous studies have demonstrated that microRNA (miR)-145 is downregulated in ovarian cancer, and that quercetin can inhibit the growth of cancer cells via regulating the expression of miRs. Therefore, the present study investigated the effect of quercetin on the expression of miR-145 in SKOV-3 and A2780 human ovarian cancer cell lines. The results revealed that the expression levels of cleaved caspase-3 in the SKOV-3 and A2780 cells were significantly increased following treatment to induce overexpression of miR-145 compared with treatment with quercetin alone (P<0.01). However, the expression of cleaved caspase-3 in the anti-miR-145 (miR-145 inhibitor) group of cells was markedly decreased compared with that in the miR-145 overexpression group (P<0.01). Taken together, the results suggested that treatment with quercetin induced the apoptosis of human ovarian carcinoma cells through activation of the extrinsic death receptor mediated and intrinsic mitochondrial apoptotic pathways.
College of Life Sciences, Huzhou Teachers College, Huzhou, Zhejiang 313000, P.R. China.
Junbo
J
Zhou
College of Life Sciences, Huzhou Teachers College, Huzhou, Zhejiang 313000, P.R. China.
Jian
J
Gong
College of Fisheries, Henan Normal University, Xinxiang, Henan 453007, P.R. China.
Chun
C
Ding
Department of Gynaecology, The Medical Group of Zhengzhou First People's Hospital, Zhengzhou, Henan 450004, P.R. China.
Guiqin
G
Chen
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D009841:Oligonucleotides / Q000235:genetics / Q000378:metabolism; D010053:Ovary / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D011794:Quercetin / Q000494:pharmacology; D053218:Receptors, Death Domain / Q000819:agonists* / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction
D000972:Antineoplastic Agents, Phytogenic; C541010:MIRN145 microRNA, human; D035683:MicroRNAs; D009841:Oligonucleotides; D053218:Receptors, Death Domain; D011794:Quercetin; D053148:Caspase 3