Sex-Biased Temporal Gene Expression in Male and Female Floral Buds of Seabuckthorn (Hippophae rhamnoides).
Seabuckthorn is an economically important dioecious plant in which mechanism of sex determination is unknown. The study was conducted to identify seabuckthorn homologous genes involved in floral development which may have role in sex determination. Forty four putative Genes involved in sex determination (GISD) reported in model plants were shortlisted from literature survey, and twenty nine seabuckthorn homologous sequences were identified from available seabuckthorn genomic resources. Of these, 21 genes were found to differentially express in either male or female flower bud stages. HrCRY2 was significantly expressed in female flower buds only while HrCO had significant expression in male flowers only. Among the three male and female floral development stages (FDS), male stage II had significant expression of most of the GISD. Information on these sex-specific expressed genes will help in elucidating sex determination mechanism in seabuckthorn.
Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat, Solan, India.
Aseem
A
Chawla
Defence Institute of High Altitude Research, Defence R & D Organisation, Leh, Jammu, and Kashmir, India.
Tsering
T
Stobdan
Defence Institute of High Altitude Research, Defence R & D Organisation, Leh, Jammu, and Kashmir, India.
Ravi B
RB
Srivastava
Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat, Solan, India.
Varun
V
Jaiswal
Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat, Solan, India.
Rajinder S
RS
Chauhan
Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat, Solan, India.
Anil
A
Kant
D056931:Cryptochromes / Q000235:genetics* / Q000378:metabolism; D035264:Flowers / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D031281:Hippophae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism
D056931:Cryptochromes; D010940:Plant Proteins