microRNA expression in autonomous thyroid adenomas: Correlation with mRNA regulation.
The objective of the study was to identify the deregulated miRNA in autonomous adenoma and to correlate the data with mRNA regulation. Seven autonomous adenoma with adjacent healthy thyroid tissues were investigated. Twelve miRNAs were downregulated and one was upregulated in the tumors. Combining bioinformatic mRNA target prediction and microarray data on mRNA regulations allowed to identify mRNA targets of our deregulated miRNAs. A large enrichment in mRNA encoding proteins involved in extracellular matrix organization and different phosphodiesterases were identified among these putative targets. The direct interaction between miR-101-3p and miR-144-3p and PDE4D mRNA was experimentally validated. The global miRNA profiles were not greatly modified, confirming the definition of these tumors as minimal deviation tumors. These results support a role for miRNA in the regulation of extracellular matrix proteins and tissue remodeling occurring during tumor development, and in the important negative feedback of the cAMP pathway, which limits the consequences of its constitutive activation in these tumors.
Institute of Interdisciplinary Research (IRIBHM), Université Libre de Bruxelles, Route de Lennik 808, 1070 Brussels, Belgium.
Sébastien L
SL
Floor
Pitié-Salpêtrière Hospital, Université Pierre et Marie Curie, Paris, France.
Christophe
C
Trésallet
Institute of Interdisciplinary Research (IRIBHM), Université Libre de Bruxelles, Route de Lennik 808, 1070 Brussels, Belgium.
Aline
A
Hébrant
Institute of Interdisciplinary Research (IRIBHM), Université Libre de Bruxelles, Route de Lennik 808, 1070 Brussels, Belgium.
Alice
A
Desbuleux
Institute of Interdisciplinary Research (IRIBHM), Université Libre de Bruxelles, Route de Lennik 808, 1070 Brussels, Belgium.
Frédérick
F
Libert
Pitié-Salpêtrière Hospital, Université Pierre et Marie Curie, Paris, France.
Catherine
C
Hoang
Hopital Erasme, Université Libre de Bruxelles, Brussels, Belgium.
Matteo
M
Capello
Institut J. Bordet, 121 Bld de Waterloo, 1000 Brussels, Belgium.
Guy
G
Andry
Institute of Interdisciplinary Research (IRIBHM), Université Libre de Bruxelles, Route de Lennik 808, 1070 Brussels, Belgium.
Wilma C G
WC
van Staveren
Institute of Interdisciplinary Research (IRIBHM), Université Libre de Bruxelles, Route de Lennik 808, 1070 Brussels, Belgium; Welbio, Université Libre de Bruxelles, Route de Lennik 808, 1070 Brussels, Belgique. Electronic address: cmaenhau@ulb.ac.be.
Carine
C
Maenhaut
D000236:Adenoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D054703:Cyclic Nucleotide Phosphodiesterases, Type 4 / Q000235:genetics* / Q000378:metabolism; D015536:Down-Regulation; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D046888:Tissue Array Analysis
C534072:MIRN101 microRNA, human; C554293:MIRN144 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D054703:Cyclic Nucleotide Phosphodiesterases, Type 4; C517376:PDE4D protein, human
Autonomous adenoma; ECM; cAMP; miRNA