Adipose-derived stromal cell cluster with light therapy enhance angiogenesis and skin wound healing in mice.
Human adipose-derived mesenchymal stem cells (hASCs) are attractive cell source for skin tissue engineering. The aim of this study was to investigate the effects of low-level light therapy (LLLT) on transplanted cluster hASC in a skin wound animal model. The hASCs were cultured in monolayer or clusters. The LLLT, hASCs, hASC clusters, and hASC clusters transplantation with LLLT (cluster + LLLT) were applied to the wound bed in athymic mice. Wound healing was assessed by gross evaluation and by hematoxylin and eosin staining, and elastin van gieson histochemistry. The survival, differentiation, and secretion of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (FGF), and hepatocyte growth factor (HGF) of the cluster ASC were evaluated by immunohistochemistry and Western blotting. The cluster + LLLT group enhanced the wound healing, including neovascularization and regeneration of skin appendages, compared with the cluster group. The secretion of growth factors was stimulated in the cluster + LLLT group compared with the ASCs and cluster group. These data suggest that LLLT is an effective biostimulator of cluster hASCs in wound healing that enhances the survival of hASCs and stimulates the secretion of growth factors in the wound bed.
Beckman Laser Institute Korea, Dankook University, 119 Dandae-ro, Cheonan, Chungnam, 330-714, Republic of Korea.
In-Su
IS
Park
Beckman Laser Institute Korea, Dankook University, 119 Dandae-ro, Cheonan, Chungnam, 330-714, Republic of Korea; Department of Otolaryngology-Head and Neck Surgery, College of Medicine, Dankook University, 119 Dandae-ro, Cheonan, Chungnam, 330-714, Republic of Korea.
Phil-Sang
PS
Chung
Beckman Laser Institute Korea, Dankook University, 119 Dandae-ro, Cheonan, Chungnam, 330-714, Republic of Korea; Department of Biomedical Science, Dankook University, Cheonan, Chungnam, 330-714, Republic of Korea; Biomedical Translational Research Institute, Dankook University, Cheonan, Chungnam, 330-714, Republic of Korea. Electronic address: jcahn@dankook.ac.kr.
Jin Chul
JC
Ahn
D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D042501:Angiogenic Proteins / Q000096:biosynthesis; D000818:Animals; D002454:Cell Differentiation; D002470:Cell Survival; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism; D006801:Humans; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D018919:Neovascularization, Physiologic; D010789:Phototherapy; D012867:Skin / Q000098:blood supply / Q000293:injuries*; D023822:Tissue Engineering / Q000379:methods; D014945:Wound Healing
D042501:Angiogenic Proteins
Adipose-derived mesenchymal stem cells; Cluster culture; Low-level light therapy; Skin regeneration; Wound healing