Genetic Changes at the Glycoprotein Editing Site Associated With Serial Passage of Sudan Virus.
Sudan virus (SUDV), like the closely related Ebola virus (EBOV), is a filovirus that causes severe hemorrhagic disease. They both contain an RNA editing site in the glycoprotein gene that controls expression of soluble and full-length protein. We tested the consequences of cell culture passage on the genome sequence at the SUDV editing site locus and determined whether this affected virulence. Passage resulted in expansion of the SUDV editing site, similar to that observed with EBOV. We compared viruses possessing either the wild-type or expanded editing site, using a nonhuman primate model of disease. Despite differences in virus serum titer at one time point, there were no significant differences in time to death or any other measured parameter. These data imply that changes at this locus were not important for SUDV lethality.
Department of Virology and Immunology, Texas Biomedical Research Institute Department of Microbiology and Immunology, University of Texas Health Science Center, San Antonio.
Kendra J
KJ
Alfson
Department of Virology and Immunology, Texas Biomedical Research Institute.
Laura E
LE
Avena
Department of Virology and Immunology, Texas Biomedical Research Institute.
Michael W
MW
Beadles
Department of Virology and Immunology, Texas Biomedical Research Institute Department of Microbiology and Immunology, University of Texas Health Science Center, San Antonio.
Heather
H
Menzie
Department of Virology and Immunology, Texas Biomedical Research Institute.
Jean L
JL
Patterson
Department of Virology and Immunology, Texas Biomedical Research Institute.
Ricardo
R
Carrion
Department of Virology and Immunology, Texas Biomedical Research Institute Department of Microbiology and Immunology, University of Texas Health Science Center, San Antonio.
Anthony
A
Griffiths
D000818:Animals; D002522:Chlorocebus aethiops; D029043:Ebolavirus / Q000235:genetics* / Q000472:pathogenicity*; D016679:Genome, Viral / Q000235:genetics; D006023:Glycoproteins / Q000235:genetics*; D000882:Haplorhini; D019142:Hemorrhagic Fever, Ebola / Q000821:virology*; D017393:RNA Editing / Q000235:genetics*; D012692:Serial Passage / Q000379:methods; D013397:Sudan; D014709:Vero Cells / Q000821:virology; D019562:Viral Load / Q000379:methods; D014774:Virulence / Q000235:genetics
D006023:Glycoproteins
RNA editing; Sudan virus; cell culture adaptation; editing site; glycoprotein