Immunoblotting conditions for small peptides from streptococci.
Streptococci secrete small peptides with important biological functions. These peptides are not amenable to standard immunoblotting, and are often detected indirectly using activity assays, or by alternative approaches that may be expensive and laborious. Here we describe an immunoblotting method that enables reproducible detection of these small streptococcal peptides.
Department of Microbiology and Immunology, Dalhousie University, Halifax, NS B3H 1X5, Canada; Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, Halifax, NS B3K 6R8, Canada.
Lauren
L
Davey
Department of Microbiology and Immunology, Dalhousie University, Halifax, NS B3H 1X5, Canada; Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, Halifax, NS B3K 6R8, Canada; Department of Pediatrics, Faculty of Medicine, Dalhousie University, IWK Health Centre, Halifax, NS B3K 6R8, Canada.
Scott A
SA
Halperin
Department of Microbiology and Immunology, Dalhousie University, Halifax, NS B3H 1X5, Canada; Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, Halifax, NS B3K 6R8, Canada; Department of Pediatrics, Faculty of Medicine, Dalhousie University, IWK Health Centre, Halifax, NS B3K 6R8, Canada; Department of Applied Oral Sciences, Faculty of Dentistry, Dalhousie University, Halifax, NS B3H 4R2, Canada. Electronic address: Song.Lee@Dal.Ca.
Song F
SF
Lee
D015151:Immunoblotting / Q000379:methods*; D010455:Peptides / Q000032:analysis*; D015203:Reproducibility of Results; D013291:Streptococcus / Q000737:chemistry*
D010455:Peptides
Bacteriocins; Competence stimulating peptides; Immunoblotting; Streptococcal small peptides