Highly sensitive fluorescent stain for detecting lipopolysaccharides in sodium dodecyl sulfate polyacrylamide gel electrophoresis.
A sensitive and simple technique was developed for the visualization of gel-separated lipopolysaccharides by using a hydrazide derivative, UGF202. As low as 0.5-1 ng total LPS could be detected by UGF202 stain, which is 2- and 16-fold more sensitive than that of the commonly used Pro-Q Emerald 300 and Keenan et al. developed silver stain, respectively. The results indicated that UGF202 stain could be a good choice for LPS determination in polyacrylamide gels.
School of Pharmaceutical Sciences, Key Laboratory of Biotechnology Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, P. R. China.|Wenzhou Undersun Biotechnology Co., Ltd, Wenzhou, Zhejiang, P. R. China.
Xu
X
Wang
School of Pharmaceutical Sciences, Key Laboratory of Biotechnology Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, P. R. China.
Ayi
A
Zhou
School of Pharmaceutical Sciences, Key Laboratory of Biotechnology Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, P. R. China.
Wanhui
W
Cai
School of Pharmaceutical Sciences, Key Laboratory of Biotechnology Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, P. R. China.
Dongdong
D
Yu
School of Pharmaceutical Sciences, Key Laboratory of Biotechnology Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, P. R. China.|Wenzhou Undersun Biotechnology Co., Ltd, Wenzhou, Zhejiang, P. R. China.
Zhongxin
Z
Zhu
School of Pharmaceutical Sciences, Key Laboratory of Biotechnology Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, P. R. China.
Chengxi
C
Jiang
School of Pharmaceutical Sciences, Key Laboratory of Biotechnology Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, P. R. China.|Wenzhou Undersun Biotechnology Co., Ltd, Wenzhou, Zhejiang, P. R. China.
Litai
L
Jin
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D007091:Image Processing, Computer-Assisted; D016014:Linear Models; D008070:Lipopolysaccharides / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D005456:Fluorescent Dyes; D008070:Lipopolysaccharides
Fluorescent stain; Lipopolysaccharides; Pro-Q Emerald 300; UGF202