Identification and molecular epidemiology of nosocomial outbreaks due to Burkholderia cepacia in cystic fibrosis patients of Masih Daneshvary Hospital, Iran.
BACKGROUND
B. cepacia complex have emerged as an important opportunistic pathogen in hospitalized and immunocompromised patients. Small hospital outbreaks are frequent and are usually due to a single contaminated environmental source. In this study we were going to investigate the role of B.cepacia complex in those patients suspected to involve with cystic fibrosis and evaluate responsible types in Masih Daneshvary Hospital.


METHODS
One hundred specimens were collected from all admitted patients who were suspected to cystic fibrosis to Masih Daneshvary hospital during one year April 2011 till end of March 2012. All were culture and identified standard procedure. All samples were checked by API system (API20NE) and by specific PCR method for genus Bulkhorderia and Bcc as well. Identified strains were finally tested by PFGE system to identifying specific involving pulse-types.


RESULTS
. Isolation and identification methods revealed 5 specimens were B.cepasia, The frequency of the cystic fibrosis detected at this study was lower than other similar study previously reported. All these isolates showed similar pattern by PFGE standard protocol that may have spread from a single source and could not be attributed to cross infections from patient to patients.


CONCLUSIONS
Application of PFGE and identification of pulse-type is a potential tool to enhance the investigation of apparent nosocomial outbreaks of B.cepacia. However it needs to be adjusted with environmental findings. Implementation of educational programs and adherence to infection control policies are obviously the main element for complete elimination of an outbreak.
M M Soltan
MM
Dallal
C F
CF
Telefian
M
M
Hajia
E
E
Kalantar
A R Dolatyar
AR
Dehkharghani
A Rahimi
AR
Forushani
Q
Q
Khanbabaei
M
M
Mobarhan
M R
MR
Farzami
D019121:Burkholderia Infections / Q000150:complications / Q000453:epidemiology* / Q000382:microbiology; D057508:Burkholderia cenocepacia / Q000235:genetics / Q000302:isolation & purification; D016956:Burkholderia cepacia / Q000235:genetics / Q000302:isolation & purification; D042602:Burkholderia cepacia complex / Q000235:genetics / Q000302:isolation & purification*; D015331:Cohort Studies; D003428:Cross Infection / Q000150:complications / Q000453:epidemiology* / Q000382:microbiology; D003550:Cystic Fibrosis / Q000150:complications*; D004269:DNA, Bacterial / Q000235:genetics*; D004196:Disease Outbreaks / Q000706:statistics & numerical data*; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006801:Humans; D007492:Iran / Q000453:epidemiology; D017720:Molecular Epidemiology; D058889:Molecular Typing; D016133:Polymerase Chain Reaction
D004269:DNA, Bacterial