Pancreatic lipase inhibitory constituents from Morus alba leaves and optimization for extraction conditions.
The leaves of Morus alba (Moraceae) have been traditionally used for the treatment of metabolic diseases including diabetes and hyperlipidemia. Thus, inhibitory effect of M. alba leaves on pancreatic lipase and their active constituents were investigated in this study. Twenty phenolic compounds including ten flavonoids, eight benzofurans, one stilbene and one chalcones were isolated from the leaves of M. alba. Among the isolated compounds, morachalcone A (20) exerted strong pancreatic lipase inhibition with IC50 value of 6.2 μM. Other phenolic compounds containing a prenyl group showed moderate pancreatic lipase inhibition with IC50 value of <50 μM. Next, extraction conditions with maximum pancreatic lipase inhibition and phenolic content were optimized using response surface methodology with three-level-three-factor Box-Behnken design. Our results suggested the optimized extraction condition for maximum pancreatic lipase inhibition and phenolic content as ethanol concentration of 74.9%; temperature 57.4 °C and sample/solvent ratio, 1/10. The pancreatic lipase inhibition and total phenolic content under optimized condition were found to be 58.5% and 26.2 μg GAE (gallic acid equivalent)/mg extract, respectively, which were well matched with the predicted value.
College of Pharmacy, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea.
Ji Yeon
JY
Jeong
College of Pharmacy, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea.
Yang Hee
YH
Jo
College of Pharmacy, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea.
Seon Beom
SB
Kim
College of Pharmacy, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea.
Qing
Q
Liu
College of Pharmacy, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea.
Jin Woo
JW
Lee
College of Pharmacy, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea.
Eun Jin
EJ
Mo
College of Pharmacy, Korea University, Sejong City 339-700, Republic of Korea.
Ki Yong
KY
Lee
College of Pharmacy, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea.
Bang Yeon
BY
Hwang
College of Pharmacy, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea. Electronic address: mklee@chungbuk.ac.kr.
Mi Kyeong
MK
Lee
D005591:Chemical Fractionation / Q000379:methods; D008049:Lipase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D015394:Molecular Structure; D031625:Morus / Q000737:chemistry*; D010179:Pancreas / Q000201:enzymology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*
D010936:Plant Extracts; D008049:Lipase
Morus alba; Optimization; Pancreatic lipase; Phenolic compounds; Response surface methodology