IL17 Mediates Pelvic Pain in Experimental Autoimmune Prostatitis (EAP).
Chronic pelvic pain syndrome (CPPS) is the most common form of prostatitis, accounting for 90-95% of all diagnoses. It is a complex multi-symptom syndrome with unknown etiology and limited effective treatments. Previous investigations highlight roles for inflammatory mediators in disease progression by correlating levels of cytokines and chemokines with patient reported symptom scores. It is hypothesized that alteration of adaptive immune mechanisms results in autoimmunity and subsequent development of pain. Mouse models of CPPS have been developed to delineate these immune mechanisms driving pain in humans. Using the experimental autoimmune prostatitis (EAP) in C57BL/6 mice model of CPPS we examined the role of CD4+T-cell subsets in the development and maintenance of prostate pain, by tactile allodynia behavioral testing and flow cytometry. In tandem with increased CD4+IL17A+ T-cells upon EAP induction, prophylactic treatment with an anti-IL17 antibody one-day prior to EAP induction prevented the onset of pelvic pain. Therapeutic blockade of IL17 did not reverse pain symptoms indicating that IL17 is essential for development but not maintenance of chronic pain in EAP. Furthermore we identified a cytokine, IL7, to be associated with increased symptom severity in CPPS patients and is increased in patient prostatic secretions and the prostates of EAP mice. IL7 is fundamental to development of IL17 producing cells and plays a role in maturation of auto-reactive T-cells, it is also associated with autoimmune disorders including multiple sclerosis and type-1 diabetes. More recently a growing body of research has pointed to IL17's role in development of neuropathic and chronic pain. This report presents novel data on the role of CD4+IL17+ T-cells in development and maintenance of pain in EAP and CPPS.
Department of Urology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, United States of America.
Stephen F
SF
Murphy
Department of Urology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, United States of America.
Anthony J
AJ
Schaeffer
Department of Urology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, United States of America.
Joseph
J
Done
Department of Urology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, United States of America.
Larry
L
Wong
Department of Urology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, United States of America.
Ashlee
A
Bell-Cohn
Department of Urology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, United States of America.
Kenny
K
Roman
Department of Urology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, United States of America.
John
J
Cashy
Department of Urology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, United States of America.
Michelle
M
Ohlhausen
Department of Urology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, United States of America.
Praveen
P
Thumbikat
D000328:Adult; D000818:Animals; D057134:Antibodies, Neutralizing / Q000494:pharmacology; D001327:Autoimmune Diseases / Q000188:drug therapy / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D015496:CD4-Positive T-Lymphocytes; D059350:Chronic Pain / Q000188:drug therapy / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D004195:Disease Models, Animal; D015870:Gene Expression; D006801:Humans; D006930:Hyperalgesia / Q000188:drug therapy / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D020381:Interleukin-17 / Q000235:genetics / Q000276:immunology*; D015851:Interleukin-7 / Q000235:genetics / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D011467:Prostate / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D011472:Prostatitis / Q000188:drug therapy / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D015398:Signal Transduction
D057134:Antibodies, Neutralizing; C508605:IL7 protein, human; D020381:Interleukin-17; D015851:Interleukin-7