[Expression of perforin and granzyme-B in peripheral blood lymphocyte in patients with prostate cancer and the clinical significance].
OBJECTIVE
To explore the expression of perforin and granzyme-B in peripheral blood lymphocyte (PBL) in patients with prostate cancer (PCa) and the clinical significance.


METHODS
The expressions of perforin and granzyme-B in PBL were detected by fluorescence quantitative reverse transcription polymerase chain reaction. The results of perforin and granzyme-B expression were compared among patients with PCa (n=60), patients with BPH (benign prostatic hyperplasia, n=40) and healthy controls (n=20).


RESULTS
Th e expressions of perforin and granzyme-B in patients with PCa were significantly lower than that in patients with BPH or that in the healthy controls (P<0.05), respectively. Furthermore, in PCa patients with low pathological grade, the expressions of perforin and granzyme-B in PBL was statistically higher than that in patients with high pathological grade (P<0.05). The expressions of perforin and granzyme-B in PCa patients at high clinical stage was statistically lower than that in PCa patients at low clinical stage (P<0.05).


CONCLUSIONS
The results of this study suggest that development and progression of PCa might be associated with poor immune status of patients.
Department of Urology, Th ird Xiangya Hospital, Central South University, Changsha 410013, China.
Jingliang
J
He
Liuxun
L
Li
Wensu
W
Wei
Jingchao
J
Wei
Zhi
Z
Long
Yichuan
Y
Zhang
Leye
L
He
D016022:Case-Control Studies; D053804:Granzymes / Q000378:metabolism*; D006801:Humans; D008214:Lymphocytes / Q000201:enzymology*; D008297:Male; D054353:Perforin / Q000378:metabolism*; D011470:Prostatic Hyperplasia; D011471:Prostatic Neoplasms / Q000276:immunology*
D054353:Perforin; D053804:Granzymes