Relationship between Proinflammatory and Antioxidant Proteins with the Severity of Cardiovascular Disease in Type 2 Diabetes Mellitus.
Type 2 diabetes mellitus patients are at significant risk of cardiovascular disease, however, the pathophysiology of these complications is complex and incompletely known in this population. The aim of this study was to compare the serum proteome of patients with type 2 diabetes mellitus presenting or not presenting cardiovascular disease with non-diabetic subjects to find essential proteins related to these cardiovascular complications. This cross-sectional study compares the serum proteome by a combination of protein depletion with 2D-DIGE (2-dimension Difference Gel Electrophoresis) methodology. The proteins differentially expressed were identified by MALDI TOF/TOF (Matrix-assisted laser desorption/ionization and Time-Of-Flight ion detector) or LC-MS/MS (Liquid Chromatography coupled to Mass-Mass Spectrometry). Type 2 diabetes mellitus patients with cardiovascular disease showed higher expression of plasma retinol binding protein and glutathione peroxidase-3 compared to those without cardiovascular disease and non-diabetic controls. These results show that proteins related to the inflammatory and redox state appear to play an important role in the pathogenesis of the cardiovascular disease in the type 2 diabetes mellitus patients.
Bone Metabolic Unit, Endocrinology Division (RETICEF), Instituto de Investigación Biosanitaria (Ibs) Granada, University Hospital San Cecilio, Granada 18012, Spain. bgfontana@fibao.es.
Beatriz
B
García-Fontana
Bone Metabolic Unit, Endocrinology Division (RETICEF), Instituto de Investigación Biosanitaria (Ibs) Granada, University Hospital San Cecilio, Granada 18012, Spain. sonia.morales.exts@juntadeandalucia.es.|Proteomic Research Service, Instituto de Investigación Biosanitaria (Ibs) Granada, University Hospital San Cecilio, Granada 18012, Spain. sonia.morales.exts@juntadeandalucia.es.
Sonia
S
Morales-Santana
Proteomic Research Service, Institute of Parasitology and Biomedicine "López Neyra" (C.S.I.C.), Granada 18016, Spain. vlongo@ipb.csic.es.
Victoria
V
Longobardo
Bone Metabolic Unit, Endocrinology Division (RETICEF), Instituto de Investigación Biosanitaria (Ibs) Granada, University Hospital San Cecilio, Granada 18012, Spain. rebeca.reyes.garcia@gmail.com.
Rebeca
R
Reyes-García
Endocrinology Division, Ciudad Real General Hospital, Ciudad Real 13005, Spain. pedrorozasm@yahoo.es.
Pedro
P
Rozas-Moreno
Infectious Diseases Unit, Instituto de Investigación Biosanitaria (Ibs) Granada, University Hospital San Cecilio, Av. Dr. Olóriz 16, Granada 18012, Spain. joseantonio.garcia@genyo.es.
José Antonio
JA
García-Salcedo
Bone Metabolic Unit, Endocrinology Division (RETICEF), Instituto de Investigación Biosanitaria (Ibs) Granada, University Hospital San Cecilio, Granada 18012, Spain. mmt@mamuto.es.|Endocrinology Unit, University Hospital San Cecilio, Av. Dr. Olóriz 16, Granada 18012, Spain. mmt@mamuto.es.
Manuel
M
Muñoz-Torres
D000328:Adult; D000886:Anthropometry; D000975:Antioxidants / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood; D002318:Cardiovascular Diseases / Q000097:blood* / Q000150:complications; D002853:Chromatography, Liquid; D003430:Cross-Sectional Studies; D048909:Diabetes Complications / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000150:complications; D015180:Electrophoresis, Gel, Two-Dimensional; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D007249:Inflammation / Q000097:blood*; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011379:Prognosis; D040901:Proteomics; D054840:Retinol-Binding Proteins, Cellular / Q000097:blood; D012720:Severity of Illness Index; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry
D000975:Antioxidants; D015415:Biomarkers; C518456:RBP1 protein, human; D054840:Retinol-Binding Proteins, Cellular; C486484:GPX3 protein, human; D005979:Glutathione Peroxidase