Structure of the K6 capsular polysaccharide from Acinetobacter baumannii isolate RBH4.
The structure of the capsular polysaccharide (CPS) from an Acinetobacter baumannii global clone 2 (GC2) clinical isolate RBH4 that carries the KL6 gene cluster was elucidated by means of chemical and spectroscopical methods. The repeating unit of K6 CPS is linear and contains N-acetyl-D-galactosamine (D-GalpNAc), two D-galactose (D-Galp) residues and 5,7-di-N-acetylpseudaminic acid (Pse5Ac7Ac). The synthesis of these sugars could be attributed to genes in the KL6 capsule biosynthesis gene cluster, and the formation of the linkages between the sugars were assigned to glycosyltransferases or the Wzy polymerase encoded in KL6.
School of Molecular Bioscience, The University of Sydney, Sydney, NSW 2006, Australia.
Johanna J
JJ
Kenyon
Department of Chemical Sciences, Complesso Universitario Monte Sant'Angelo, Napoli, Italy.
Alberto M
AM
Marzaioli
School of Molecular Bioscience, The University of Sydney, Sydney, NSW 2006, Australia.
Ruth M
RM
Hall
Department of Chemical Sciences, Complesso Universitario Monte Sant'Angelo, Napoli, Italy. Electronic address: decastro@unina.it.
Cristina
C
De Castro
D040981:Acinetobacter baumannii / Q000737:chemistry* / Q000235:genetics; D009682:Magnetic Resonance Spectroscopy; D005810:Multigene Family / Q000235:genetics; D013400:Sugar Acids / Q000737:chemistry
C513444:5,7-diacetamido-3,5,7,9-tetradeoxynonulosonic acid; D013400:Sugar Acids
Capsular polysaccharide; K locus; KL6 gene cluster; NMR spectroscopy; Pseudaminic acid