Integrated systems for exosome investigation.
Extracellular vesicles, including exosomes, are currently being investigated to better understand their biogenesis and biological functions. There is also a rapidly growing interest in utilizing exosomes present in patient biofluids for molecular diagnostics in the clinic. Exosomes are natural shuttles of RNA and protein cargo, making them attractive as potential therapeutic delivery vehicles. Here, we describe the methods for using the latest tools and technologies to study exosomes to better understand their roles in cell-to-cell communication, for discovery of clinical biomarkers and to engineer exosomes for therapeutic applications.
System Biosciences (SBI), 265 N. Whisman Rd., Mountain View, CA 94043, USA.
Maureen F
MF
Peterson
System Biosciences (SBI), 265 N. Whisman Rd., Mountain View, CA 94043, USA.
Nicole
N
Otoc
System Biosciences (SBI), 265 N. Whisman Rd., Mountain View, CA 94043, USA.
Jasmine K
JK
Sethi
System Biosciences (SBI), 265 N. Whisman Rd., Mountain View, CA 94043, USA.
Archana
A
Gupta
System Biosciences (SBI), 265 N. Whisman Rd., Mountain View, CA 94043, USA. Electronic address: tantes@systembio.com.
Travis J
TJ
Antes
D014408:Biomarkers, Tumor / Q000235:genetics / Q000302:isolation & purification*; D002458:Cell Fractionation / Q000295:instrumentation / Q000379:methods; D002850:Chromatography, Gel; D055354:Exosomes / Q000737:chemistry*; D005427:Flocculation; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000302:isolation & purification*; D008854:Microscopy, Electron; D009363:Neoplasm Proteins / Q000235:genetics / Q000302:isolation & purification*; D009369:Neoplasms / Q000737:chemistry* / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification*; D011933:Reagent Kits, Diagnostic; D014461:Ultracentrifugation; D014462:Ultrafiltration
D014408:Biomarkers, Tumor; D035683:MicroRNAs; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm; D011933:Reagent Kits, Diagnostic
Exosomes; Extracellular vesicles; Liquid biopsy; Nanobiomedicine; Oncosomes; miRNA biomarkers