Short Communication: Expression of APOBEC3G and Interferon Gamma in Pleural Fluid Mononuclear Cells from HIV/TB Dual Infected Subjects.
Sites of HIV/TB coinfection are characterized by increased HIV-1 replication and a TH1 profile. However, expression of HIV-1 restriction factors, such as APOBEC3G (A3G) in situ, is unknown. Using an RT-profiler focused on genes related to HIV-1 expansion, we examined pleural fluid mononuclear cells (PFMCs) from patients with HIV/TB coinfection in comparison to HIV-uninfected patients with TB disease. Significant expression of interferon (IFN)-γ and restriction factors A3G and A3F and TRIM5α in PFMCs was found. Genes correlating significantly with the expression of IFN-γ included A3G and A3F. However, pleural fluid HIV-1 viral load and HIV-1 gag/pol mRNA in PFMCs did not correlate with A3G activity.
1 Case Western Reserve University , Cleveland, Ohio.|2 Veterans Affairs Medical Center , Cleveland, Ohio.
Zahra
Z
Toossi
1 Case Western Reserve University , Cleveland, Ohio.
Qinglai
Q
Meng
1 Case Western Reserve University , Cleveland, Ohio.
Htin
H
Aung
1 Case Western Reserve University , Cleveland, Ohio.
Shigou
S
Liu
1 Case Western Reserve University , Cleveland, Ohio.|3 Makerere University , Kampala, Uganda .|4 Joint Clinical Research Center , Kampala, Uganda .
Harriet
H
Mayanja-Kizza
1 Case Western Reserve University , Cleveland, Ohio.
Christina S
CS
Hirsch
D000071480:APOBEC-3G Deaminase; D000328:Adult; D001826:Body Fluids / Q000166:cytology* / Q000821:virology; D003564:Cytidine Deaminase / Q000096:biosynthesis*; D020869:Gene Expression Profiling; D015658:HIV Infections / Q000150:complications / Q000276:immunology* / Q000821:virology; D015497:HIV-1 / Q000302:isolation & purification; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008297:Male; D010996:Pleural Effusion; D014397:Tuberculosis, Pulmonary / Q000150:complications / Q000276:immunology*; D019562:Viral Load
D007371:Interferon-gamma; D000071480:APOBEC-3G Deaminase; C464924:APOBEC3G protein, human; D003564:Cytidine Deaminase