Determination of biomarkers for polycyclic aromatic hydrocarbons (PAHs) toxicity to earthworm (Eisenia fetida).
Polycyclic aromatic hydrocarbon (PAH) compounds are persistent, carcinogenic, and mutagenic. When PAHs enter agricultural soils through sewage sludge, they pose an environmental risk to soil organisms, including earthworms. Therefore, we aimed to determine the toxic effects of PAHs on earthworms. Five PAHs were used: fluorene, anthracene, phenanthrene, fluoranthene, and pyrene. Only fluorene and phenanthrene exhibited toxicity (LC50 values 394.09 and 114.02 g L(-1), respectively) against the earthworm Eisenia fetida. None of the other PAHs tested in this study enhanced the mortality of adult earthworm until the concentrations reached to 1000 g L(-1). After exposure to PAHs, acetylcholinesterase (AChE) activity in E. fetida decreased in a concentration-dependent manner, and phenanthrene exhibited the strongest inhibitory effect on AChE, followed by fluorene. Activity of a representative detoxifying enzyme, carboxylesterase, was dramatically reduced in E. fetida exposed to all tested PAHs in comparison with that observed in the control test. The remaining glutathione S-transferase activity significantly decreased in E. fetida after exposure to PAHs. To profile small proteins <20 kDa, SELDI-TOF MS with Q10 ProteinChips was used, and 54 proteins were identified as being significantly different from the control (p = 0.05). Among them, the expressions of three proteins at 4501.8, 4712.4, and 4747.9 m/z were only enhanced in E. fetida exposed to anthracene and pyrene. One protein with 16,174 m/z was selectively expressed in E. fetida exposed to fluorene, phenanthrene, and fluoranthene. These proteins may be potential biomarkers for the five PAHs tested in E. fetida.
School of Applied Biosciences, Kyungpook National University, Daegu, 702-701, Korea.
Tae-Hoon
TH
Nam
School of Applied Biosciences, Kyungpook National University, Daegu, 702-701, Korea.
Hwang-Ju
HJ
Jeon
Division of Environmental Science and Ecological Engineering, Korea University, Seoul, 136-701, Korea.
Hyung-ho
HH
Mo
Division of Environmental Science and Ecological Engineering, Korea University, Seoul, 136-701, Korea.
Kijong
K
Cho
Department of Biological Environment, Korea Biochar Research Center, Kangwon National University, Chuncheon, 200-701, Korea.
Yong-Sik
YS
Ok
School of Applied Biosciences, Kyungpook National University, Daegu, 702-701, Korea. selpest@knu.ac.kr.
Sung-Eun
SE
Lee
D000818:Animals; D000873:Anthracenes / Q000633:toxicity; D015415:Biomarkers / Q000032:analysis; D005449:Fluorenes / Q000633:toxicity; D009835:Oligochaeta / Q000187:drug effects* / Q000378:metabolism; D010616:Phenanthrenes / Q000633:toxicity; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity*; D020543:Proteome / Q000032:analysis; D011721:Pyrenes / Q000633:toxicity; D012989:Soil Pollutants / Q000633:toxicity*
D000873:Anthracenes; D015415:Biomarkers; D005449:Fluorenes; D010616:Phenanthrenes; D011084:Polycyclic Aromatic Hydrocarbons; D020543:Proteome; D011721:Pyrenes; D012989:Soil Pollutants; C007738:fluoranthene; C041509:fluorene; C031181:phenanthrene; C030984:pyrene; C034020:anthracene
Biomarker; Polycyclic aromatic hydrocarbons; SELDI-TOF MS