AtRBP1, which encodes an RNA-binding protein containing RNA-recognition motifs, regulates root growth in Arabidopsis thaliana.
AtRBP1 is an RNA-binding protein containing RNA-recognition motifs in Arabidopsis thaliana, homologues of which are not observed in metazoa. Transgenic plants expressing artificial microRNAs for repressing AtRBP1 expression displayed a stunted primary root phenotype during germination. Transgenic plants overexpressing AtRBP1 also displayed the same phenotype. Tight regulation of the AtRBP1 transcript may be required for normal root growth. An in vitro binding assay showed that AtRBP1 preferentially binds to sequences containing UUAGG, GUAGG and/or UUAGU. In vivo selection of RNAs bound to AtRBP1 suggests that transcripts of At3g06780, At4g15910, At5g11760 and At5g07350 are target RNAs of AtRBP1.
Biosystems Science Course, Graduate School of Life Science, Hokkaido University, Sapporo 060-0810, Japan.
Takuhiro
T
Shida
Department of Biological Sciences, Faculty of Science, Hokkaido University, Sapporo 060-0814, Japan.
Ai
A
Fukuda
Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, Chiyoda-ku, Tokyo 120-8554, Japan.
Tamao
T
Saito
Department of Biological Sciences, Faculty of Science, Hokkaido University, Sapporo 060-0814, Japan; Japan Science and Technology Agency, PRESTO, Kawaguchi, Saitama 332-0012, Japan.
Hidetaka
H
Ito
Department of Biological Sciences, Faculty of Science, Hokkaido University, Sapporo 060-0814, Japan. Electronic address: atsushi@sci.hokudai.ac.jp.
Atsushi
A
Kato
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018525:Germination; D035683:MicroRNAs / Q000378:metabolism; D008969:Molecular Sequence Data; D010641:Phenotype; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism; D030821:Plants, Genetically Modified; D018749:RNA, Plant / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism
D029681:Arabidopsis Proteins; D035683:MicroRNAs; D018749:RNA, Plant; D016601:RNA-Binding Proteins
Arabidopsis thaliana; RNA ligand; RNA-binding protein; RNA-recognition motif; Root growth