Cryopreservation for preservation of potato genetic resources.
Cryopreservation is becoming a very important tool for the long-term storage of plant genetic resources and efficient cryopreservation protocols have been developed for a large number of plant species. Practical procedures, developed using in vitro tissue culture, can be a simple and reliable preservation option of potato genetic resources rather than maintaining by vegetative propagation in genebanks due their allogamous nature. Cryopreserved materials insure a long-term backup of field collections against loss of plant germplasm. Occurrence of genetic variation, in tissue culture cells during prolonged subcultures, can be avoided with suitable cryopreservation protocols that provide high regrowth, leading and facilitating a systematic and strategic cryo-banking of plant genetic resources. Cryopreservation protocols for potato reviewed here, can efficiently complement field and in vitro conservation, providing for preservation of genotypes difficult to preserve by other methods, wild types and other species decided as priority collections.
Gene Research Center, University of Tsukuba , Tsukuba, Ibaraki 305-8572 , Japan.
Takao
T
Niino
Gene Research Center, University of Tsukuba , Tsukuba, Ibaraki 305-8572 , Japan ; Centro Nacional de Recursos Genéticos, INIFAP , Tepatitlán de Morelos 47600 , México.
Miriam Valle
MV
Arizaga
D cryo-plate; DMSO droplet; V cryo-plate; cryopreservation; droplet vitrification; encapsulation vitrification; potato genetic resource