[An ELISA system based on the specific class Y (IgY) antibodies from egg yolks for the quantitative determination of D-antigen in inactivated poliovirus vaccines].
The results of the construction of the first Russian ELISA system for the quantitative determination of D-antigen of 1-3 poliovirus types in the preparations of inactivated poliovirus vaccines are presented. For the first time, this kind of system is based on the use of specific antibodies of class Y (IgY) from egg yolks of immunized hens. It was shown that this ELISA system is specific, sufficiently sensitive, and can be used for quantitative determination of D-antigen of 1-3 poliovirus types in inactivated poliovirus vaccines.
A P
AP
Ivanov
V G
VG
Kozlov
T D
TD
Klebleeva
O E
OE
Ivanova
A V
AV
Kiktenko
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis* / Q000096:biosynthesis / Q000302:isolation & purification; D000956:Antigens, Viral / Q000276:immunology*; D002645:Chickens / Q000276:immunology; D004530:Egg Yolk / Q000737:chemistry / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards; D005260:Female; D006801:Humans; D007114:Immunization; D007136:Immunoglobulins / Q000032:analysis* / Q000096:biosynthesis / Q000302:isolation & purification; D011051:Poliomyelitis / Q000175:diagnosis* / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D017955:Poliovirus / Q000276:immunology; D011054:Poliovirus Vaccine, Inactivated / Q000008:administration & dosage; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral; D000956:Antigens, Viral; C016512:IgY; D007136:Immunoglobulins; D011054:Poliovirus Vaccine, Inactivated