Yulangsan polysaccharide improves redox homeostasis and immune impairment in D-galactose-induced mimetic aging.
Yulangsan polysaccharide (YLSP) is a traditional Chinese medicine used in long-term treatment as a modulator of brain dysfunction and immunity. In this study, we evaluated the protective effect of YLSP against D-galactose-induced impairment of oxidative stress and the immune system and evaluated its possible mechanism of action. D-galactose was subcutaneously injected into the dorsal neck of mice daily for 8 weeks to establish the aging model. YLSP was simultaneously administered once daily. The results indicate that YLSP significantly improves the general appearance of the aging mice. YLSP significantly increased the levels of antioxidant enzymes, such as super oxide dismutase, glutathione peroxidase, catalase and total anti-oxidation capability, while decreasing the content of malondialdehyde in different tissues, including the liver, brain, and serum. YLSP also increased the interleukin-2 level while decreasing the interleukin-6 level. Moreover, YLSP significantly inhibited advanced glycation end product formation. Furthermore, YLSP decreased p21 and p53 gene expressions in the liver and brain of D-galactose-treated mice. These results suggest that YLSP may have a protective effect suppressing the aging process by enhancing antioxidant activity and immunity, as well as modulating aging-related gene expression.
Department of Pharmacology, Guangxi Medical University, Nanning 530021, PR China. huangrenbin518@163.com.
Van Minh
VM
Doan
Chunxia
C
Chen
Xing
X
Lin
Van Phuc
VP
Nguyen
Zhihuan
Z
Nong
Weisi
W
Li
Qingquan
Q
Chen
Jianjun
J
Ming
Qiuqiao
Q
Xie
Renbin
R
Huang
D000375:Aging / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D000818:Animals; D002374:Catalase / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005690:Galactose / Q000009:adverse effects*; D005979:Glutathione Peroxidase / Q000378:metabolism; D017127:Glycation End Products, Advanced / Q000378:metabolism; D006706:Homeostasis / Q000187:drug effects; D006801:Humans; D007107:Immune System / Q000187:drug effects* / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D033641:Millettia / Q000737:chemistry*; D010084:Oxidation-Reduction / Q000187:drug effects; D011134:Polysaccharides / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D017127:Glycation End Products, Advanced; D007376:Interleukin-2; D015850:Interleukin-6; D011134:Polysaccharides; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005690:Galactose