Comparative proteomic analysis of serum diagnosis patterns of sputum smear-positive pulmonary tuberculosis based on magnetic bead separation and mass spectrometry analysis.
A major challenge in pulmonary tuberculosis (TB) control is early and accurate diagnosis of sputum smear negative pulmonary TB (SSN-PTB). The patients with SSN-PTB have to wait for a longer period of time before receiving proper treatment than sputum smear positive pulmonary TB (SSP-PTB) patients due to delay in diagnosis. The purpose of this study is to discover potential serum protein biomarkers for SSN-PTB. Surface-enhanced laser desorption/ionization-time of flight mass spectrometry (SELDI-TOF MS) combined with weak cation exchange (WCX) magnetic beads was used to screen serum samples from SSN-PTB patients (N = 66), SSP-PTB patients (N = 49), and healthy volunteers (N = 80). The serum protein profiles were analyzed with Biomarker Wizard system. A classification model was established using Biomarker Pattern Software (BPS). Fifty-eight protein peaks were identified to exhibit significant differences between SSN-PTB, SSP-PTB and healthy control groups (P < 0.05), among which 6 peaks were found to be down-regulated, while 10 peaks were up-regulated gradually in the healthy control, SSN-PTB, and SSP-PTB groups. Twenty-three discriminating m/z peaks were detected between SSN-PTB patients and healthy controls (P < 0.01, Fold ≥ 1.5). The classification tree combined with three protein peaks (2747.0, 4480.0, and 9410.1 Da) could distinguish SSN-PTB patients from healthy controls with a sensitivity of 83.33% and a specificity of 82.50%. Early diagnosis of SSN-PTB disease is critical in order to reduce morbidity and mortality associated with TB. The study will help to clarify the role of differential proteins in the pathogenesis of TB.
College of Life and Environmental Sciences, Hangzhou Normal University Hangzhou, P. R. China.
Jiyan
J
Liu
Institute of Cell Biology, Zhejiang University Hangzhou, P. R. China.
Tingting
T
Jiang
Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine Beijing, P. R. China.
Feng
F
Jiang
Institute of Cell Biology, Zhejiang University Hangzhou, P. R. China.
Dandan
D
Xu
Department of Respiratory Medicine, The Sixth Hospital of Shaoxing Shaoxing, P. R. China.
Liliang
L
Wei
Institute of Cell Biology, Zhejiang University Hangzhou, P. R. China.
Chong
C
Wang
Institute of Cell Biology, Zhejiang University Hangzhou, P. R. China.
Zhongliang
Z
Chen
Institute of Cell Biology, Zhejiang University Hangzhou, P. R. China.
Xing
X
Zhang
Institute of Cell Biology, Zhejiang University Hangzhou, P. R. China.
Jicheng
J
Li
Biomarker; Proteomic; sputum smear negative pulmonary tuberculosis; surface-enhanced laser desorption/ionization