Multivalent aptamer-RNA based fluorescent probes for carrier-free detection of cellular microRNA-34a in mucin1-expressing cancer cells.
In this study, multivalent carrier-free aptamer-RNA based fluorescent probes (CF-probes) were designed as a simpler, more reliable, timesaving strategy for cellular miRNA detection. CF-probes spontaneously delivered into cells without the need for additional carriers and visualized target microRNA-34a specifically.
Department of Bioscience and Biotechnology, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 143-701, Republic of Korea. hjmok@konkuk.ac.kr.
Jihyun
J
Kim
Eunjoo
E
Lee
Yoon Young
YY
Kang
Hyejung
H
Mok
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D045744:Cell Line, Tumor; D004591:Electrophoresis, Polyacrylamide Gel; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D061986:MCF-7 Cells; D035683:MicroRNAs / Q000032:analysis* / Q000097:blood / Q000652:urine; D018613:Microscopy, Confocal; D018396:Mucin-1 / Q000378:metabolism*; D009841:Oligonucleotides / Q000737:chemistry; D013050:Spectrometry, Fluorescence
D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D035683:MicroRNAs; D018396:Mucin-1; D009841:Oligonucleotides; C477371:locked nucleic acid