[The establishment of Raji drug-resistant cell line and analyses of prohibitin and miR-27a expression].
OBJECTIVE
To establish Raji adriamycin (ADR)-resistant cell lines and analysis the expression of mitochondria Prohibitin (PHB) and microRNA-27a (miR-27a), as well as discuss its clinical significance.


METHODS
Built ADR-resistant Raji cells, detected their resistant index and drug-resistant spectrum and stability, observed their morphology and growth characteristics in general; evaluated the expression of phb mRNA and miR-27a in ADR-resistant cells (Raji/A) and sensitive cells (Raji/S) via real-time quantitative polymerase chain reaction (RT-PCR).


RESULTS
The ADR-resistant Raji cell lines were built; expression of PHB1 mRNA in Raji/A was evidently higher than in Raji/S (P<0.05), the expressing difference of PHB2 mRNA in Raji/A and Raji/S was statistically meaningless (P>0.05), the expression of miR-27a in Raji/A was much higher than in Raji/S (P<0.05).


CONCLUSIONS
By building the experimental model of Raji ADR-resistant cell lines, high expression level of PHB1 and miR-27a were detected in the cell lines, indicating that PHB1 and miR-27a may be associated with ADR-resistance of Raji cells.
Department of Hematology, Provincial Hospital Affiliated to Shandong University, Ji'nan 250021, China.
Rongli
R
Zheng
Department of Hematology, Provincial Hospital Affiliated to Shandong University, Ji'nan 250021, China.
Yujie
Y
Jiang
Department of Hematology, Provincial Hospital Affiliated to Shandong University, Ji'nan 250021, China.
Xin
X
Wang
D045744:Cell Line, Tumor; D004317:Doxorubicin; D019008:Drug Resistance, Neoplasm; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs; D000091402:Prohibitins; D012333:RNA, Messenger; D012097:Repressor Proteins
D035683:MicroRNAs; C000717328:PHB protein, human; C000717331:PHB2 protein, human; D000091402:Prohibitins; D012333:RNA, Messenger; D012097:Repressor Proteins; D004317:Doxorubicin