Biological activities of Peristrophe bivalvis extracts: promising potential for anti-snake venoms against Naja kaouthia and Trimeresurus albolabris venoms.
This study evaluates the in vitro anti-snake venom potential of Peristrophe bivalvis (PB) extracts against Naja kaouthia (NK) and Trimeresurus albolabris (TA) venoms, including inhibition of cytotoxic effects and enzymatic activities, and the binding-precipitation of extracts and venom proteins analysis. In addition, the antioxidant, cytotoxic and in vivo acute oral toxic activities of PB extracts are also reported. The in vitro cytotoxic and enzymatic analysis reveals that the ethanol extracts of stems and leaves of PB showed good anti-snake venom activity against NK and TA venoms. In addition, the antioxidant result indicated that only the ethanol extract of leaves exhibited weak DPPH radical-scavenging activity. The ethanol whole-plant extract of PB also showed no cytotoxicity against four cell lines. Moreover, the in vivo acute oral toxicity result of the ethanol whole-plant extract showed that all treated rats did not exhibit abnormal toxic signs or deaths.
a Faculty of Science and Technology, Rajamungala University of Technology Thanyaburi , Thanyaburi , Pathum Thani , 12110 , Thailand.
Jatuporn
J
Phaopongthai
b Research and Development Department , Queen Saovabha Memorial Institute , Pathumwan, Bangkok 10330 , Thailand.
Jureeporn
J
Noiphrom
a Faculty of Science and Technology, Rajamungala University of Technology Thanyaburi , Thanyaburi , Pathum Thani , 12110 , Thailand.
Supat
S
Phaopongthai
b Research and Development Department , Queen Saovabha Memorial Institute , Pathumwan, Bangkok 10330 , Thailand.
Narumol
N
Pakmanee
c Natural Products Research Unit, Department of Chemistry, Faculty of Science, Chulalongkorn University , Bangkok 10330 , Thailand.
Jirapast
J
Sichaem
D027901:Acanthaceae / Q000737:chemistry*; D000818:Animals; D000997:Antivenins / Q000494:pharmacology*; D018938:Caco-2 Cells; D002522:Chlorocebus aethiops; D017815:Elapidae; D005347:Fibroblasts / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012910:Snake Venoms / Q000037:antagonists & inhibitors* / Q000201:enzymology; D018675:Toxicity Tests; D017841:Trimeresurus; D014709:Vero Cells
D000997:Antivenins; D010936:Plant Extracts; D012910:Snake Venoms
Peristrophe bivalvis; acute oral toxicity; anti-snake venom activity; antioxidant activity; cytotoxicity