Big endothelin-1 as a tumour marker for canine haemangiosarcoma.
Haemangiosarcoma (HSA) is an important malignant neoplasm of dogs that originates from vascular endothelial cells. This study explored the suitability of using serum big endothelin-1 (ET-1) as a tumour marker for canine spontaneous HSA. Serum big ET-1 was measured in dogs with splenic HSA (n = 14), splenic malignant tumours other than HSA (n = 10), benign splenic lesions (n = 11) and normal healthy dogs (n = 17) by ELISA. Serum big ET-1 levels in dogs with HSA were significantly (P < 0.01) higher than in other dogs. High sensitivity (100%, 95% confidence interval 86-100%) and specificity (95%, 95% confidence interval 86-95%) for HSA diagnosis were obtained using a cut-off of 17 pg/mL according to receiver operating characteristic (ROC) curves (area under ROC curve 0.93). PPET1, ETA, VEGF and Hif1-α mRNA expression, measured by real-time PCR, were elevated in HSA compared with normal tissues. These findings suggest that elevated serum big ET-1 could be used as a diagnostic marker for canine HSA.
Veterinary Internal Medicine, Department of Small Animal Clinical Sciences, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan.
Shinya
S
Fukumoto
Companion Animal Nutrition, Department of Veterinary Sciences, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan.
Taku
T
Miyasho
Veterinary Internal Medicine, Department of Small Animal Clinical Sciences, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan.
Kiwamu
K
Hanazono
Institute for Biomedical Research and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Ibaraki 305-8566, Japan.
Kaname
K
Saida
Veterinary Oncology, Department of Small Animal Clinical Sciences, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan.
Tsuyoshi
T
Kadosawa
Veterinary Biochemistry, Department of Basic Veterinary Medicine, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan.
Hidetomo
H
Iwano
Veterinary Internal Medicine, Department of Small Animal Clinical Sciences, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan. Electronic address: uchide@rakuno.ac.jp.
Tsuyoshi
T
Uchide
D000818:Animals; D014408:Biomarkers, Tumor / Q000097:blood; D016022:Case-Control Studies; D004283:Dog Diseases / Q000097:blood* / Q000175:diagnosis / Q000378:metabolism; D004285:Dogs; D019332:Endothelin-1 / Q000097:blood* / Q000235:genetics / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006394:Hemangiosarcoma / Q000097:blood / Q000378:metabolism / Q000662:veterinary*; D013160:Splenic Neoplasms / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*
D014408:Biomarkers, Tumor; D019332:Endothelin-1
Big endothelin-1; Canine; Haemangiosarcoma; Oncology; Tumour marker