MiR-31 modulates coelomocytes ROS production via targeting p105 in Vibrio splendidus challenged sea cucumber Apostichopus japonicus in vitro and in vivo.
MiR-31 is a critical regulator of gene expression in many pathogenic processes in vertebrates. In this study, we identified p105 as a novel target of miR-31 in Apostichopus japonicus and investigated their regulatory roles in vitro and in vivo. The negative expression profiles between miR-31 and Ajp105 were detected in both LPS-exposed primary coelomocytes and Vibrio splendidus-challenged sea cucumber. Co-infection miR-31 mimics significantly depressed the expression of Ajp105 and increased ROS production in vitro. In contrast, miR-31 inhibitor significantly elevated the expression of Ajp105 and decreased ROS level. Consistently, miR-31 over-expression or Ajp105 silencing in vivo both greatly promoted ROS accumulation. Taken together, our findings confirmed that miR-31 could modulate respiratory burst via targeting Ajp105 during sea cucumber pathological development.
School of Marine Sciences, Ningbo University, Ningbo, Zhejiang Province 315211, PR China.
Meng
M
Lu
School of Marine Sciences, Ningbo University, Ningbo, Zhejiang Province 315211, PR China.
Pengjuan
P
Zhang
School of Marine Sciences, Ningbo University, Ningbo, Zhejiang Province 315211, PR China. Electronic address: lichenghua@nbu.edu.cn.
Chenghua
C
Li
School of Marine Sciences, Ningbo University, Ningbo, Zhejiang Province 315211, PR China.
Weiwei
W
Zhang
School of Marine Sciences, Ningbo University, Ningbo, Zhejiang Province 315211, PR China.
Chunhua
C
Jin
School of Marine Sciences, Ningbo University, Ningbo, Zhejiang Province 315211, PR China.
Qingxi
Q
Han
D000818:Animals; D019295:Computational Biology; D005786:Gene Expression Regulation; D007113:Immunity, Innate; D008070:Lipopolysaccharides / Q000494:pharmacology; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D047528:Stichopus / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D051193:Toll-Like Receptors / Q000235:genetics / Q000378:metabolism; D014733:Vibrio / Q000502:physiology
D008070:Lipopolysaccharides; D035683:MicroRNAs; D017382:Reactive Oxygen Species; D051193:Toll-Like Receptors
Apostichopus japonicus; MiR-31; Respiratory burst; p105