HCC-DETECT: a combination of nuclear, cytoplasmic, and oncofetal proteins as biomarkers for hepatocellular carcinoma.
Currently, the search for suitable hepatocellular carcinoma (HCC) biomarkers is very intensive. Besides, efficacy and cost/effectiveness of screening and surveillance of cirrhotics for the diagnosis of HCC is still debated. So, the present study is concerned with the evaluation of cytokeratin-1 (CK-1) and nuclear matrix protein-52 (NMP-52) for identifying HCC. Two-hundred and eighty individuals categorized into three groups [liver fibrosis (F1-F3), cirrhosis (F4), and HCC] constituted this study. Western blot was used for identifying CK-1 and NMP-52 in serum samples. As a result, a single immunoreactive band was shown at 67 and 52 kDa corresponding to CK-1 and NMP-52, respectively. Both CK-1 and NMP-52 bands were cut and electroeluted separately. These markers were quantified in sera using ELISA. Patients with HCC were associated with higher concentrations of CK-1 and NMP-52 than those without HCC with a significant difference (P < 0.0001). CK-1 showed an area under receiver-operating characteristic curve (AUC) of 0.83 with 75 % sensitivity and 82 % specificity while NMP-52 yielded 0.72 AUC with 62 % sensitivity and 70 % specificity for identifying HCC. HCC-DETECT comprising CK-1 and NMP-52 together with AFP was then constructed yielding 0.90 AUC for identifying HCC with 80 % sensitivity and 92 % specificity. HCC-DETECT was then tested for separating HCC from F1-F3 showing 0.94 AUC with 80 % sensitivity and 93 % specificity. In conclusion, CK-1 in conjunction with NMP-52 and AFP could have a potential role for improving the detection of HCC with a high degree of accuracy.
Biotechnology Research Center, P.O. Box (14), 23 July St., Industrial Zone, New Damietta, 34517, Egypt. amattallah@hotmail.com.
Abdelfattah M
AM
Attallah
Faculty of Science, Mansoura University, Mansoura, Egypt.
Mohamed
M
El-Far
Faculty of Science, Damietta University, Mansoura, Egypt.
Camelia A Abdel
CA
Malak
Faculty of Science, Helwan University, Cairo, Egypt.
Mohamed M
MM
Omran
Faculty of Medicine, Mansoura University, Mansoura, Egypt.
Gamal E
GE
Shiha
Faculty of Medicine, Mansoura University, Mansoura, Egypt.
Khaled
K
Farid
Faculty of Science, Port-Said University, Port-Said, Egypt.
Lamiaa A
LA
Barakat
Biotechnology Research Center, P.O. Box (14), 23 July St., Industrial Zone, New Damietta, 34517, Egypt.
Mohamed S
MS
Albannan
Biotechnology Research Center, P.O. Box (14), 23 July St., Industrial Zone, New Damietta, 34517, Egypt.
Ahmed A
AA
Attallah
Biotechnology Research Center, P.O. Box (14), 23 July St., Industrial Zone, New Damietta, 34517, Egypt.
Mohamed A
MA
Abdelrazek
Biotechnology Research Center, P.O. Box (14), 23 July St., Industrial Zone, New Damietta, 34517, Egypt.
Mohamed S
MS
Elbendary
Faculty of Science, Damietta University, Mansoura, Egypt.
Refaat
R
Sabry
Biotechnology Research Center, P.O. Box (14), 23 July St., Industrial Zone, New Damietta, 34517, Egypt.
Gehan A
GA
Hamoda
Biotechnology Research Center, P.O. Box (14), 23 July St., Industrial Zone, New Damietta, 34517, Egypt.
Mohamed M
MM
Elshemy
Biotechnology Research Center, P.O. Box (14), 23 July St., Industrial Zone, New Damietta, 34517, Egypt.
Abdallah A
AA
Ragab
Biotechnology Research Center, P.O. Box (14), 23 July St., Industrial Zone, New Damietta, 34517, Egypt.
Basma M
BM
Foda
Faculty of Science, Cairo University, Giza, Egypt.
Sanaa O
SO
Abdallah
D000328:Adult; D000951:Antigens, Neoplasm / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism*; D005260:Female; D006801:Humans; D007633:Keratins / Q000378:metabolism; D008103:Liver Cirrhosis / Q000378:metabolism; D008113:Liver Neoplasms / Q000175:diagnosis / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D034521:Nuclear Matrix-Associated Proteins / Q000378:metabolism; D012372:ROC Curve; D012680:Sensitivity and Specificity
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D034521:Nuclear Matrix-Associated Proteins; C031192:oncofetal antigens; D007633:Keratins
HCC-DETECT; Liver diseases; Serum biomarkers; Western blot