The effect of amorphous calcium phosphate on protein protection against thermal denaturation.
The hybrid nanoparticles of amorphous calcium phosphate (ACP)-catalase (CAT) developed by in situ biomineralization can create a stable semi-aqueous nanoscale environment for entrapped proteins against thermal denaturation. This finding indicates the importance of an amorphous mineral phase in the preservation of organic macromolecules.
Centre for Biomaterials and Biopathways, Department of Chemistry, Zhejiang University, Hangzhou, Zhejiang 310027, China. rtang@zju.edu.cn.
Yuling
Y
Yang
Guangchuan
G
Wang
Genxing
G
Zhu
Xurong
X
Xu
Haihua
H
Pan
Ruikang
R
Tang
D002130:Calcium Phosphates / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D002374:Catalase / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D008958:Models, Molecular; D010316:Particle Size; D011489:Protein Denaturation / Q000187:drug effects; D055550:Protein Stability / Q000187:drug effects; D013499:Surface Properties; D013696:Temperature
D002130:Calcium Phosphates; C020243:calcium phosphate; D002374:Catalase