Effects of DHA-rich fish oil supplementation on the lipid profile, markers of muscle damage, and neutrophil function in wheelchair basketball athletes before and after acute exercise.
We investigated the effects of docosahexaenoic acid (DHA)-rich fish oil (FO) supplementation on the lipid profile, levels of plasma inflammatory mediators, markers of muscle damage, and neutrophil function in wheelchair basketball players before and after acute exercise. We evaluated 8 male basketball wheelchair athletes before and after acute exercise both prior to (S0) and following (S1) FO supplementation. The subjects were supplemented with 3 g of FO daily for 30 days. The following components were measured: the plasma lipid profile (total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, and triglycerides), plasma inflammatory mediators (C-reactive protein, interleukin (IL)-1β, IL-1ra, IL-4, IL-6, IL-8, and tumor necrosis factor-α), markers of muscle damage (creatine kinase and lactate dehydrogenase (LDH)), and neutrophil function (cytokine production, phagocytic capacity, loss of membrane integrity, mitochondrial membrane potential, neutral lipid accumulation, phosphatidylserine externalization, DNA fragmentation, and production of reactive oxygen species (ROS)). Acute exercise increased the plasma levels of total cholesterol, LDH, IL1ra, and IL-6, led to the loss of membrane integrity, ROS production, and a high mitochondrial membrane potential in neutrophils, and reduced the phagocytic capacity and IL-6 production by the neutrophils (S0). However, supplementation prevented the increases in the plasma levels of LDH and IL-6, the loss of membrane integrity, and the alterations in ROS production and mitochondrial membrane potential in the neutrophils that were induced by exercise (S1). In conclusion, DHA-rich FO supplementation reduces the markers of muscle damage, inflammatory disturbances, and neutrophil death induced by acute exercise in wheelchair athletes.
Institute of Physical Activity and Sports Sciences, Post-Graduate Program in Human Movement Science, Cruzeiro do Sul University, Rua Galvão Bueno, 868, CEP 01506-000, São Paulo, SP, Brazil.
Camila Garcia
CG
Marques
Vinicius Coneglian
VC
Santos
Adriana Cristina
AC
Levada-Pires
Thiago Manzoni
TM
Jacintho
Renata
R
Gorjão
Tânia Cristina
TC
Pithon-Curi
Maria Fernanda
MF
Cury-Boaventura
D000273:Adipose Tissue / Q000378:metabolism; D000328:Adult; D056352:Athletes; D001490:Basketball; D015415:Biomarkers / Q000097:blood*; D015992:Body Mass Index; D002097:C-Reactive Protein / Q000378:metabolism; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D003402:Creatine Kinase / Q000378:metabolism; D053938:DNA Fragmentation; D019587:Dietary Supplements; D015444:Exercise; D005395:Fish Oils / Q000008:administration & dosage*; D006801:Humans; D053583:Interleukin-1beta / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D016209:Interleukin-8 / Q000097:blood; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D064133:Sports Nutritional Physiological Phenomena; D014280:Triglycerides / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D014910:Wheelchairs
D015415:Biomarkers; C508606:CXCL8 protein, human; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D005395:Fish Oils; C508585:IL1B protein, human; C508598:IL4 protein, human; C508600:IL6 protein, human; D053583:Interleukin-1beta; D015850:Interleukin-6; D016209:Interleukin-8; D017382:Reactive Oxygen Species; D014280:Triglycerides; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4; D002097:C-Reactive Protein; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase
acide docosahexanoïque; acide eicosapentaénoïque; acides gras; docosahexaenoic acid; eicosapentaenoic acid; fatty acids; immune system; lésion médullaire; neutrophil; neutrophiles; spinal cord injury; système immunitaire