Dynamic contrast enhanced magnetic resonance imaging of an orthotopic pancreatic cancer mouse model.
Dynamic contrast enhanced magnetic resonance imaging (DCE-MRI) has been limitedly used for orthotopic pancreatic tumor xenografts due to severe respiratory motion artifact in the abdominal area. Orthotopic tumor models offer advantages over subcutaneous ones, because those can reflect the primary tumor microenvironment affecting blood supply, neovascularization, and tumor cell invasion. We have recently established a protocol of DCE-MRI of orthotopic pancreatic tumor xenografts in mouse models by securing tumors with an orthogonally bent plastic board to prevent motion transfer from the chest region during imaging. The pressure by this board was localized on the abdominal area, and has not resulted in respiratory difficulty of the animals. This article demonstrates the detailed procedure of orthotopic pancreatic tumor modeling using small animals and DCE-MRI of the tumor xenografts. Quantification method of pharmacokinetic parameters in DCE-MRI is also introduced. The procedure described in this article will assist investigators to apply DCE-MRI for orthotopic gastrointestinal cancer mouse models.
Radiology, University of Alabama at Birmingham; hyunki@uab.edu.
Hyunki
H
Kim
Radiology, University of Alabama at Birmingham.
Sharon
S
Samuel
Radiology, University of Alabama at Birmingham.
John W
JW
Totenhagen
Radiology, University of Alabama at Birmingham.
Marie
M
Warren
Comprehensive Cancer Center, University of Alabama at Birmingham.
Jeffrey C
JC
Sellers
Radiation Oncology, University of Alabama at Birmingham.
Donald J
DJ
Buchsbaum
D000818:Animals; D003287:Contrast Media / Q000493:pharmacokinetics; D004195:Disease Models, Animal; D005260:Female; D064593:Heterografts; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D016513:Mice, SCID; D009389:Neovascularization, Pathologic / Q000473:pathology; D010190:Pancreatic Neoplasms / Q000098:blood supply / Q000378:metabolism / Q000473:pathology*
D003287:Contrast Media