Gene polymorphisms of tumor necrosis factor alpha and antioxidant enzymes in bronchial asthma.
BACKGROUND
Bronchial asthma is an inflammatory disease resulting from a combination of genetic and environmental factors. Single nucleotide polymorphisms in the regulatory regions of cytokine and antioxidant enzyme genes may affect cytokine production and enzyme activity, and thus play a contributory role in asthma pathogenesis.


OBJECTIVE
The aim of this study was to examine the association of manganese superoxide dismutase (MnSOD) Ala16Val, catalase (CAT) A-21T and tumor necrosis factor alpha (TNF-α) G-308A polymorphisms with bronchial asthma.


METHODS
A total of 79 patients with asthma and 95 healthy controls were screened for MnSOD Ala16Val, CAT A-21T and TNF-α G-308A polymorphisms using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.


RESULTS
The results obtained showed significantly higher prevalence of the MnSOD ValVal genotype (χ2=14.463, df=2, p=0.001) and MnSOD 16Val allele (χ2=12.862, p=0.026, OR=0.451, 95% CI=0.291-0.699) in patients with asthma compared to controls. The genotype and allele frequencies distribution of CAT A-21T and TNF-α G-308A gene polymorphisms did not show differences between patients and controls.


CONCLUSIONS
Our results show an association of MnSOD Ala16Val genetic polymorphism with asthma in a Serbian population and suggest a protective role of the MnSOD 16Ala allele.
Department of Biochemistry, Faculty of Medicine, University of Nis, Serbia.
Milena
M
Despotovic
Department of Biochemistry, Faculty of Medicine, University of Nis, Serbia.
Tatjana J
TJ
Stoimenov
Clinic for Pulmonary Diseases and Tuberculosis, Nis Clinical Center, University of Nis, Knez Selo, Serbia.
Ivana
I
Stankovic
Department of Biochemistry, Faculty of Medicine, University of Nis, Serbia.
Dusica
D
Pavlovic
Department of Biochemistry, Faculty of Medicine, University of Nis, Serbia.
Dusan
D
Sokolovic
Department of Biochemistry, Faculty of Medicine, University of Nis, Serbia.
Tatjana
T
Cvetkovic
Department of Biochemistry, Faculty of Medicine, University of Nis, Serbia.
Gordana
G
Kocic
Department of Biochemistry, Faculty of Medicine, University of Nis, Serbia.
Jelena
J
Basic
Department of Biochemistry, Faculty of Medicine, University of Nis, Serbia.
Andrej
A
Veljkovic
Department of Biochemistry, Faculty of Medicine, University of Nis, Serbia.
Branka
B
Djordjevic
D000328:Adult; D001249:Asthma / Q000175:diagnosis / Q000201:enzymology / Q000235:genetics* / Q000276:immunology; D016022:Case-Control Studies; D002374:Catalase / Q000235:genetics; D016009:Chi-Square Distribution; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D010641:Phenotype; D011110:Polymorphism, Genetic; D065840:Protective Factors; D012307:Risk Factors; D055771:Serbia; D013482:Superoxide Dismutase / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D000099156:Superoxide Dismutase 2
D002374:Catalase; D013482:Superoxide Dismutase; D014409:Tumor Necrosis Factor-alpha; D000099156:Superoxide Dismutase 2