Gastroprotective effect of andrographolide sodium bisulfite against indomethacin-induced gastric ulceration in rats.
Andrographolide sodium bisulfite (ASB), a water-soluble sulfonate of andrographolide has been shown to possess anti-inflammatory, antipyretic and analgesic activities. However, there is no report on the gastroprotective effect of ASB against indomethacin-induced gastric ulcer. Here we investigated the possible anti-ulcerogenic potential of ASB and the underlying mechanism against indomethacin-induced gastric ulcer in rats. The ulcer area, histopathological assessment, contents of gastric mucosal glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), malonaldehyde (MDA) and prostaglandin E2 (PGE2) were examined. In addition, cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2) mRNA expression and immunohistochemical evaluation of HSP70, Bcl-2 and Bax proteins were also investigated. Results indicated that ASB pre-treatment significantly reduced the ulcer areas induced by indomethacin compared with the vehicle group. The gastric levels of GSH, CAT and SOD were markedly increased by ASB while the level of MDA was decreased. In addition, ASB pretreatment significantly promoted the gastric PGE2 levels and up-regulated the COX-1 and COX-2 mRNA expression in comparison with the vehicle group. Immunohistochemistry analysis showed obvious up-regulation of HSP70 and Bcl-2 protein expression while suppression of Bax protein in the gastric tissue of ASB-pretreated group. Taken together, these findings indicated that the gastroprotective effect of ASB might be associated with the improvement of antioxidative status, activation of COX-mediated PGE2 synthesis, down-regulation of Bax proteins and up-regulation of Bcl-2 and HSP70 proteins. ASB might have the potential for further development as a promising alternative for antiulcer treatment.
School of Chinese Medicines, Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China.
Yu-Hong
YH
Liu
School of Chinese Medicines, Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China.
Zhen-Biao
ZB
Zhang
School of Chinese Medicines, Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China.
Yi-Feng
YF
Zheng
School of Chinese Medicines, Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China.
Hai-Ming
HM
Chen
The First Affiliated Hospital of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510405, PR China.
Xiu-Ting
XT
Yu
School of Chinese Medicines, Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China.
Xiao-Ying
XY
Chen
School of Chinese Medicines, Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China.
Xie
X
Zhang
The Second Affiliated Hospital of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510120, PR China.
Jian-Hui
JH
Xie
School of Chinese Medicines, Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China.
Zu-Qing
ZQ
Su
School of Chinese Medicines, Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China.
Xue-Xuan
XX
Feng
The First Affiliated Hospital of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510405, PR China. Electronic address: gancaozhf@126.com.
Hui-Fang
HF
Zeng
School of Chinese Medicines, Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China; Dongguan Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Dongguan 523808, PR China. Electronic address: suziren@gzucm.edu.cn.
Zi-Ren
ZR
Su
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000009:adverse effects; D002374:Catalase / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D004224:Diterpenes / Q000008:administration & dosage* / Q000009:adverse effects; D005753:Gastric Mucosa / Q000187:drug effects* / Q000473:pathology; D005978:Glutathione / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007213:Indomethacin / Q000008:administration & dosage; D008315:Malondialdehyde / Q000378:metabolism; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013276:Stomach Ulcer / Q000139:chemically induced / Q000517:prevention & control*; D013447:Sulfites / Q000008:administration & dosage* / Q000009:adverse effects; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D004224:Diterpenes; D018840:HSP70 Heat-Shock Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D013447:Sulfites; C030419:andrographolide; D008315:Malondialdehyde; D002374:Catalase; D011451:Prostaglandin-Endoperoxide Synthases; D013482:Superoxide Dismutase; D005978:Glutathione; D015232:Dinoprostone; C009279:sodium bisulfite; D007213:Indomethacin
Andrographolide sodium bisulfite; Antioxidant; Apoptosis; Gastric ulcer; Indomethacin; Rats