Blood Parameters and Toxicity of Chromium Picolinate Oral Supplementation in Lambs.
The effects of oral supplementation of chromium picolinate (CrPic) on various blood parameters and their possible toxicity on the liver, kidneys, lungs, heart, and testis were investigated. Twenty-four Santa Inês (SI) lambs were treated with four different concentrations of CrPic (six animals/treatment): placebo, 0.250, 0.375, and 0.500 mg CrPic/animal/day for 84 days. The basal diet consisted of hay Panicum maximum cv Massai and concentrate. Blood and serum were collected fortnightly for analysis. On day 84, the animals were euthanized, and histopathological analysis in the liver, kidney, heart, lung, and testis was made. The liver and kidney were also submitted to electronic microscopy analysis. Differences between treatments (P < 0.05) were observed for packed cell volume (day 84), hemoglobin (day 84), total plasm protein (day 56 and day 84), and triglycerides (day 70). There was no statistically significant relationship between Cr supplementation and histopathology findings, although some animals treated with supplementary Cr showed morphological changes in the liver, kidney, and testis. Thus, the effectiveness of supplementation with Cr remains in doubt as to its physiological action and toxicity in sheep.
Faculdade de Agronomia e Medicina Veterinária/UnB, Campus Darcy Ribeiro, ICC-Sul, Brasília, DF, Brazil, 70910-900. dallago@unb.br.|Instituto Nacional de Ciência e Tecnologia-Informação Genético-Sanitária da Pecuária Brasileira (CNPq/INCT/IGSPB), Belo Horizonte, MG, Brazil. dallago@unb.br.
Bruno Stéfano Lima
BS
Dallago
Laboratório de Microscopia Eletrônica, Insitituto de Ciências Biológicas/UnB, Campus Darcy Ribeiro, Brasília, DF, Brazil, 70910-900. shelidabraz@gmail.com.
ShélidaVasconcelos
S
Braz
Laboratório de Patologia Clínica Veterinária/UnB, Campus Darcy Ribeiro, ICC-Sul, Brasília, DF, Brazil, 70910-900. tatigmarcola@hotmail.com.
Tatiana Guerrero
TG
Marçola
Faculdade de Agronomia e Medicina Veterinária/UnB, Campus Darcy Ribeiro, ICC-Sul, Brasília, DF, Brazil, 70910-900. concepta@unb.br.|Instituto Nacional de Ciência e Tecnologia-Informação Genético-Sanitária da Pecuária Brasileira (CNPq/INCT/IGSPB), Belo Horizonte, MG, Brazil. concepta@unb.br.
Concepta
C
McManus
Diretoria de Defesa e Vigilância Agropecuária, Secretaria de Agricultura e Desenvolvimento Rural-SEAGRI/DF, Parque Estação Biológica-Asa Norte, Brasília, DF, Brazil, 70770-914. denise_caldeira@hotmail.com.
Denise Ferreira
DF
Caldeira
Centro de Energia Nuclear na Agricultura/USP, Av. Centenário, no 303, Caixa Postal 96, Piracicaba, SP, Brazil, 13400-970. aline.campeche@gmail.com.
Aline
A
Campeche
Centro de Energia Nuclear na Agricultura/USP, Av. Centenário, no 303, Caixa Postal 96, Piracicaba, SP, Brazil, 13400-970. icedtoad@gmail.com.
Edgard Franco
EF
Gomes
Instituto Federal de Educação, Ciência e Tecnologia Goiano, Campus Iporá, Av. Oeste s/n, Iporá, GO, Brazil, 76200-000. pradopaim@hotmail.com.
Tiago Prado
TP
Paim
Faculdade de Agronomia e Medicina Veterinária/UnB, Campus Darcy Ribeiro, ICC-Sul, Brasília, DF, Brazil, 70910-900. barbaraoliveirab@hotmail.com.
Bárbara Oliveira
BO
Borges
Centro de Energia Nuclear na Agricultura/USP, Av. Centenário, no 303, Caixa Postal 96, Piracicaba, SP, Brazil, 13400-970. louvandini@cena.usp.br.|Instituto Nacional de Ciência e Tecnologia-Informação Genético-Sanitária da Pecuária Brasileira (CNPq/INCT/IGSPB), Belo Horizonte, MG, Brazil. louvandini@cena.usp.br.
Helder
H
Louvandini
D000284:Administration, Oral; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D001772:Blood Cell Count; D001786:Blood Glucose / Q000032:analysis; D004032:Diet; D019587:Dietary Supplements; D008055:Lipids / Q000097:blood; D008297:Male; D008897:Panicum; D010848:Picolinic Acids / Q000097:blood* / Q000633:toxicity*; D034561:Sheep, Domestic; D014018:Tissue Distribution
D001786:Blood Glucose; D008055:Lipids; D010848:Picolinic Acids; C030614:picolinic acid
Hemogram; Leukogram; Mineral supplement; Serum biochemistry