CpsA, a LytR-CpsA-Psr Family Protein in Mycobacterium marinum, Is Required for Cell Wall Integrity and Virulence.
LytR-CpsA-Psr family proteins play an important role in bacterial cell wall integrity. Although the pathogenic relevance of LytR-CpsA-Psr family proteins has been studied in a few bacterial pathogens, their function in mycobacteria remains uncharacterized. In this work, a transposon insertion mutant (cpsA::Tn) of Mycobacterium marinum was studied. We found that inactivation of CpsA altered bacterial colony morphology, sliding motility, cell surface hydrophobicity, and cell wall permeability. Besides, the cpsA mutant exhibited a decreased arabinogalactan content, indicating that CpsA plays a role in cell wall assembly. Moreover, the mutant shows impaired growth within macrophage cell lines and is severely attenuated in zebrafish larvae and adult zebrafish. Taken together, our results indicated that CpsA, a previously uncharacterized protein, is important for mycobacterial cell wall integrity and is required for mycobacterial virulence.
Key Laboratory of Medical Molecular Virology of MOE and MOH, Institutes of Biomedical Sciences and Institute of Medical Microbiology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, China.
Qinglan
Q
Wang
Key Laboratory of Medical Molecular Virology of MOE and MOH, Institutes of Biomedical Sciences and Institute of Medical Microbiology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, China.
Lin
L
Zhu
Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA.
Victoria
V
Jones
Key Laboratory of Medical Molecular Virology of MOE and MOH, Institutes of Biomedical Sciences and Institute of Medical Microbiology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, China.
Chuan
C
Wang
Key Laboratory of Medical Molecular Virology of MOE and MOH, Institutes of Biomedical Sciences and Institute of Medical Microbiology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, China.
Yifei
Y
Hua
Key Laboratory of Medical Molecular Virology of MOE and MOH, Institutes of Biomedical Sciences and Institute of Medical Microbiology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, China.
Xujun
X
Shi
Key Laboratory of Medical Molecular Virology of MOE and MOH, Institutes of Biomedical Sciences and Institute of Medical Microbiology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, China.
Xia
X
Feng
Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA.
Mary
M
Jackson
Key Laboratory of Medical Molecular Virology of MOE and MOH, Institutes of Biomedical Sciences and Institute of Medical Microbiology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, China.
Chen
C
Niu
Key Laboratory of Medical Molecular Virology of MOE and MOH, Institutes of Biomedical Sciences and Institute of Medical Microbiology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, China qgao99@yahoo.com.
Qian
Q
Gao
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D004251:DNA Transposable Elements; D057927:Hydrophobic and Hydrophilic Interactions; D007814:Larva / Q000382:microbiology; D008124:Locomotion; D008264:Macrophages / Q000276:immunology / Q000382:microbiology; D051379:Mice; D016254:Mutagenesis, Insertional; D019910:Mycobacterium marinum / Q000254:growth & development / Q000378:metabolism / Q000472:pathogenicity / Q000502:physiology*; D010539:Permeability; D014774:Virulence; D015027:Zebrafish / Q000382:microbiology
D001426:Bacterial Proteins; D004251:DNA Transposable Elements