Molecular detection of Capillaria philippinensis: An emerging zoonosis in Egypt.
Human infection with Capillaria philippinensis is accidental; however, it may end fatally if not diagnosed and treated in the proper time. The first case was detected in the Philippines in 1963, but later reported in other countries around the world, including Egypt. In this report, molecular diagnosis using a specific nested PCR for detection of C. philippinensis in faeces is described based on the amplification of small ribosomal subunit. The test showed sensitivity and specificity, as it detected all the positive cases and gave no cross-reaction with human DNA and DNA of other tested parasites. This method can be very useful not only for improvement of diagnosis, but also to understand the different environmental routes of transmission by detection of C. philippinensis DNA-stages in the possible fish intermediate hosts and reservoir animal host, helping to improve strategies for surveillance and prevention of human disease.
Department of Medical Parasitology, Kasr Al-Ainy Faculty of Medicine, Cairo University, Egypt.
Nadia A
NA
El-Dib
Department of Medical Parasitology, Kasr Al-Ainy Faculty of Medicine, Cairo University, Egypt. Electronic address: aelbadry@kasralainy.edu.eg.
Ayman A
AA
El-Badry
Malaria & Emerging Parasitic Diseases Laboratory, Parasitology Department, National Microbiology Center, Carlos III Health Institute, Cra. Majadahonda Pozuelo Km. 2,5 Majadahonda, 28220 Madrid, Spain.
Thuy-Huong
TH
Ta-Tang
Malaria & Emerging Parasitic Diseases Laboratory, Parasitology Department, National Microbiology Center, Carlos III Health Institute, Cra. Majadahonda Pozuelo Km. 2,5 Majadahonda, 28220 Madrid, Spain.
Jose M
JM
Rubio
D000818:Animals; D001483:Base Sequence; D002195:Capillaria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D021821:Communicable Diseases, Emerging / Q000175:diagnosis* / Q000453:epidemiology / Q000635:transmission; D018509:DNA, Helminth / Q000032:analysis / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000032:analysis / Q000302:isolation & purification; D004197:Disease Reservoirs / Q000469:parasitology; D004534:Egypt / Q000453:epidemiology; D017189:Enoplida Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000635:transmission; D005243:Feces / Q000469:parasitology; D005260:Female; D006801:Humans; D007814:Larva / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D010063:Ovum; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012335:RNA, Ribosomal / Q000737:chemistry; D012680:Sensitivity and Specificity; D016415:Sequence Alignment; D015047:Zoonoses / Q000175:diagnosis* / Q000453:epidemiology / Q000469:parasitology / Q000635:transmission
D018509:DNA, Helminth; D004275:DNA, Ribosomal; D012335:RNA, Ribosomal
Capillaria philippinensis; Capillariasis; Copro-DNA; Mal-absorption; Nested PCR