Characterization of the release profile of doxycycline by PLGA microspheres adjunct to non-surgical periodontal therapy.
The aim of this pilot study was to assess the release of locally delivered doxycycline by poly (l-lactide-co-glycolide) (PLGA) microspheres in the periodontal pocket of patients with chronic periodontitis, treated by non-surgical periodontal therapy. Nineteen sites of non-adjacent teeth of four different patients were evaluated. Five milligram of PLGA microspheres loaded with 16 doxycycline hyclate (DOX) was administered per periodontal site. To quantify DOX released into the periodontal pocket, gingival crevicular fluid (GCF) was collected from the sites on days 2, 5, 7, 10, 15, and 20 after DOX application, and high-performance liquid chromatography was performed. Data were statistically assessed by ANOVA/Tukey test. At days 2, 5, and 7, the DOX concentration was stably sustained (23.33 ± 1.38, 23.4 ± 1.82, and 22.75 ± 1.33 μg/mL, respectively), with no significant differences over these assessment times (p > 0.05). At days 10 and 15, a tendency was observed toward a decrease in DOX concentration (21.74 ± 0.91 and 20.53 ± 4.88 μg/mL, respectively), but a significant decrease in GCF drug concentration (19.69 ± 4.70 μg/mL) was observed only on day 20. The DOX delivery system developed demonstrated a successful sustained release after local administration, as an adjunct to non-surgical periodontal therapy.
a Division of Periodontics, Department of Prosthodontics and Periodontics, Piracicaba Dental School , State University of Campinas (UNICAMP) , Av. Limeira 901, Caixa Postal: 052, CEP: 13414-903 Piracicaba , São Paulo , Brazil.
Lucas Alves
LA
Moura
Fernanda Vieira
FV
Ribeiro
Talita Bianchi
TB
Aiello
Eliana Ap De Rezende
EA
Duek
Enilson Antonio
EA
Sallum
Francisco Humberto
FH
Nociti Junior
Márcio Zaffalon
MZ
Casati
Antonio Wilson
AW
Sallum
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D004318:Doxycycline / Q000737:chemistry* / Q000494:pharmacology* / Q000627:therapeutic use; D004337:Drug Carriers / Q000737:chemistry*; D065546:Drug Liberation; D005883:Gingival Crevicular Fluid / Q000187:drug effects; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D008863:Microspheres; D010514:Periodontal Pocket / Q000188:drug therapy*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer
D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D004318:Doxycycline
FTIR; HPLC; PLGA; delivery system; doxycycline; microspheres; non-surgical periodontal therapy