Effects of local nasal immunotherapy in allergic airway inflammation: Using urea denatured Dermatophagoides pteronyssinus.
Despite improvements in anti-allergy medication, the prevalence of allergic airway inflammation remains high, affecting up to 40% of the population worldwide. Allergen immunotherapy is effective for inducing tolerance but has the adverse effect of severe allergic reaction. This can be avoided by denaturing with urea. In this study, we demonstrated that the serum level of allergen-specific IgE in mice sensitized with native Dermatophagoides pteronyssinus (Der p) crude extract after receiving local nasal immunotherapy (LNIT) with urea-denatured Der p crude extract (DN-Dp) significantly decreased compared to that in the normal saline (NS) treatment group. Expressions of IL-4 were significantly reduced in lung tissues after treatment. Inflammation around the bronchial epithelium improved and airway hypersensitivity was down-regulated. LNIT with DN-Dp can down-regulate IL-1b, IL-6 and TNF-a expression and then decrease Der p-induced allergic airway inflammation. This therapeutic modality may be used as an alternative treatment for airway allergic diseases.
a Institute of Biomedical Sciences; National Chung Hsing University ; Taichung , Taiwan.
Sheng-Jie
SJ
Yu
En-Chih
EC
Liao
Jaw-Ji
JJ
Tsai
D000281:Administration, Intranasal; D000485:Allergens / Q000737:chemistry* / Q000276:immunology*; D000818:Animals; D015153:Blotting, Western; D040002:Dermatophagoides pteronyssinus / Q000276:immunology*; D003888:Desensitization, Immunologic / Q000379:methods*; D005260:Female; D006967:Hypersensitivity / Q000097:blood / Q000276:immunology / Q000628:therapy*; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007150:Immunohistochemistry; D053583:Interleukin-1beta / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D014508:Urea / Q000737:chemistry*
D000485:Allergens; D007074:Immunoglobulin G; D053583:Interleukin-1beta; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D007073:Immunoglobulin E; D014508:Urea
Allergic airway inflammation; DEX, dexamethasone; DN-Dp, Urea denatured Dermatophagoides pteronyssinus crude extract; Der p, Dermatophagoides pteronyssinus; Dermatophagoides pteronyssinus; LNIT, Local nasal immunotherapy; allergy; local nasal immunotherapy; urea denatured allergen