HISTONE DEACETYLASE6 Controls Gene Expression Patterning and DNA Methylation-Independent Euchromatic Silencing.
To investigate the role of chromatin regulators in patterning gene expression, we employed a unique epigenetically controlled and highly tissue-specific green fluorescent protein reporter line in Arabidopsis (Arabidopsis thaliana). Using a combination of forward and reverse genetic approaches on this line, we show here that distinct epigenetic regulators are involved in silencing the transgene in different tissues. The forward genetic screen led to the identification of a novel HISTONE DEACETYLASE6 (HDA6) mutant allele (epigenetic control1, hda6-8). This allele differs from the previously reported alleles, as it did not affect DNA methylation and only had a very modest effect on the release of transposable elements and other heterochromatic transcripts. Overall, our data shows that HDA6 has at least two clearly separable activities in different genomic regions. In addition, we present an unexpected role for HDA6 in the control of DNA methylation at CG dinucleotides.
Botanical Institute, Zürich-Basel Plant Science Center, University of Basel, CH-4056 Basel, Switzerland (E.H., K.F., L.K., E.B.); andUniversité d'Angers, UMR1345 Institut de Recherche en Horticulture et Semences, 49071 Beaucouzé, France (D.W., E.B.).
Emilija
http://orcid.org/0000-0003-3323-114X
E
Hristova
Botanical Institute, Zürich-Basel Plant Science Center, University of Basel, CH-4056 Basel, Switzerland (E.H., K.F., L.K., E.B.); andUniversité d'Angers, UMR1345 Institut de Recherche en Horticulture et Semences, 49071 Beaucouzé, France (D.W., E.B.).
Kateryna
K
Fal
Botanical Institute, Zürich-Basel Plant Science Center, University of Basel, CH-4056 Basel, Switzerland (E.H., K.F., L.K., E.B.); andUniversité d'Angers, UMR1345 Institut de Recherche en Horticulture et Semences, 49071 Beaucouzé, France (D.W., E.B.).
Laurin
http://orcid.org/0000-0002-1062-7363
L
Klemme
Botanical Institute, Zürich-Basel Plant Science Center, University of Basel, CH-4056 Basel, Switzerland (E.H., K.F., L.K., E.B.); andUniversité d'Angers, UMR1345 Institut de Recherche en Horticulture et Semences, 49071 Beaucouzé, France (D.W., E.B.).
David
D
Windels
Botanical Institute, Zürich-Basel Plant Science Center, University of Basel, CH-4056 Basel, Switzerland (E.H., K.F., L.K., E.B.); andUniversité d'Angers, UMR1345 Institut de Recherche en Horticulture et Semences, 49071 Beaucouzé, France (D.W., E.B.) etienne.bucher@angers.inra.fr.
Etienne
http://orcid.org/0000-0002-3114-3763
E
Bucher
D000107:Acetylation; D000483:Alleles; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D002843:Chromatin / Q000235:genetics*; D019175:DNA Methylation; D004251:DNA Transposable Elements / Q000235:genetics; D044127:Epigenesis, Genetic; D022041:Euchromatin / Q000235:genetics; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D006655:Histone Deacetylases / Q000235:genetics / Q000378:metabolism; D006657:Histones / Q000235:genetics / Q000378:metabolism; D009928:Organ Specificity; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics; D016415:Sequence Alignment; D019076:Transgenes
D029681:Arabidopsis Proteins; D002843:Chromatin; D004251:DNA Transposable Elements; D022041:Euchromatin; D006657:Histones; C432452:AT5G63110 protein, Arabidopsis; D006655:Histone Deacetylases