First record of Limnatispaluda (Hirudinida, Arhynchobdellida, Praobdellidae) from Kazakhstan, with comments on genetic diversity of Limnatis leeches.
BACKGROUND
Sawyer (1986) included three species in the nasal leech genus LimnatisMoquin-Tandon 1827: Limnatisnilotica (Savigny 1822), LimnatisbacescuiManoleli 1972 and Limnatispaluda (Tennent 1859). The first and last species have mainly been identified in Middle Eastern countries (e.g. Kinzelbach and Rückert 1985). The second species has been identified only in Romania Dobruja (Manoleli 1972). Although Limnatis leeches are well known species of endoparasitic leeches, Limnatisnilotica was recorded only once in Kazakhstan (Lukin 1976).


CONCLUSIONS
Specimens of the genus Limnatis from Almaty Province, Kazakhstan are identified as Limnatispaluda. This is the first record of Limnatispaluda from Kazakhstan. Mitochondrial COI and 12S data demonstrated that the present specimens are genetically close to an Israeli specimen identified as Limnatisnilotica. In addition, molecular data suggest that some Limnatis specimens whose DNA sequences have been reported were misidentified. According to the observed phylogenetic relationships, the taxonomic status of the known Limnatis species should be revisited.
Graduate School of Education, Hiroshima University, Higashihiroshima, Japan ; Graduate School of Science, Kyoto University, Kyoto, Japan.
Takafumi
T
Nakano
Institute of Zoology MES RK, Almaty, Kazakhstan ; Association for the Conservation of Biodiversity of Kazakhstan (ACBK), Astana, Kazakhstan.
Tatjana
T
Dujsebayeva
Graduate School of Human and Environmental Studies, Kyoto University, Kyoto, Japan.
Kanto
K
Nishikawa
12S; COI; Hirudinea; Hirudinida; Kazakhstan; Limnatispaluda; genetic diversity; geographical record