Effect of sulfur dioxide inhalation on the expression of KATP and L-Ca(2+) channels in rat hearts.
Epidemiological studies have revealed an association between sulfur dioxide (SO2) exposure and cardiovascular diseases. This study is designed to investigate the SO2 effect on the expression of ATP-sensitive K(+) (KATP) channel and L-type calcium (L-Ca(2+)) channel in rat hearts. The results show that the mRNA and protein levels of the KATP channel subunits Kir6.2 and SUR2A of rat hearts in SO2 groups were higher than those in control group. SO2 at 14mg/m(3) significantly decreased the expression of the L-Ca(2+) channel subunits Cav1.2 and Cav1.3. This suggests that SO2 can activate the KATP channels by up-regulating the expression of Kir6.2 and SUR2A, while it inhibits the L-Ca(2+) channels by down-regulating the expression of Cav1.2 and Cav1.3 in rat hearts. The molecular mechanism of SO2-induced negative inotropic effect might be linked to the expression changes of these subunits, which may contribute to the pathogenesis of SO2-associated cardiovascular diseases.
Institute of Environmental Medicine and Toxicology, Institute of Environmental Science, Shanxi University, Taiyuan 030006, China. Electronic address: qxzhang@sxu.edu.cn.
Quanxi
Q
Zhang
Institute of Environmental Medicine and Toxicology, Institute of Environmental Science, Shanxi University, Taiyuan 030006, China.
Yunlong
Y
Bai
Institute of Environmental Medicine and Toxicology, Institute of Environmental Science, Shanxi University, Taiyuan 030006, China.
Zhenhua
Z
Yang
Institute of Environmental Medicine and Toxicology, Institute of Environmental Science, Shanxi University, Taiyuan 030006, China.
Jingjing
J
Tian
Institute of Environmental Medicine and Toxicology, Institute of Environmental Science, Shanxi University, Taiyuan 030006, China.
Ziqiang
Z
Meng
D000818:Animals; D020746:Calcium Channels, L-Type / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D006321:Heart / Q000187:drug effects*; D001239:Inhalation; D054086:KATP Channels / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D008297:Male; D024661:Potassium Channels, Inwardly Rectifying / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D064233:Sulfonylurea Receptors / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D013458:Sulfur Dioxide / Q000633:toxicity*; D015854:Up-Regulation; D000099161:Kcnj11 Channel
D020746:Calcium Channels, L-Type; D054086:KATP Channels; D024661:Potassium Channels, Inwardly Rectifying; D064233:Sulfonylurea Receptors; D013458:Sulfur Dioxide; D000099161:Kcnj11 Channel
Cardiovascular diseases; Gene expression; K(ATP) channel; L-Ca(2+) channel; Sulfur dioxide