[Co-inhibition effect of 17-DMAG and oxaliplatin on proliferation and invasion of colorectal cancer cells].
OBJECTIVE
To explore the effect of heat shock protein 90 (HSP90) inhibitor (17-DMAG) and oxaliplatin on the proliferation and invasion of colorectal cancer.


METHODS
After 17-DMAG, oxaliplatin and half-dose combination of 2 drugs processing colorectal cancer SW480 and HCT116 cell lines, CCK8 assay was applied to detect cell viability. RT-PCR and Western blot were used to detect the expression level of the apoptosis-related molecules. Transwell chemokine axis experiment and Western blot were employed to detect cell invasion ability and the expression level of tumor metastasis-associated protein.


RESULTS
The growth of SW480 and HCT116 cells was inhibited after the administration of 17-DMAG and oxaliplatin(P<0.05) in dose- and time-dependent manner. Processed by 17-DMAG 100 nmol/L, oxaliplatin 50 mg/L and half-dose combination of 2 drugs, transcription level of the apoptosis inhibitory gene (Bcl-2) in SW480 and HCT116 cells was decreased, the level of apoptosis promoting gene (Bax) transcription and protein PARP-1 spliceosome expression was increased, and the above trend was more obvious when using half-dose combination of 2 drugs. Transwell chemokine axis experiments showed the penetrating relative percentage and expression level of MMP9 and integrin β3 decreased, especially for half-dose combination of 2 drugs.


CONCLUSIONS
17-DMAG and oxaliplatin can co-inhibit the proliferation and invasion of colorectal cancer.
Department of General Surgery, the First Affiliated Hospital with Nanjing Medical University, Nanjing 210029, China. xuzekuan@njmu.edu.cn.
Jianping
J
Zhou
Weimin
W
Wang
Jianliang
J
Deng
Yan
Y
Zhou
Lulu
L
Wu
Zhiyuan
Z
Guo
Jianneng
J
Shi
Jun
J
Shi
Sujun
S
Zhou
Zekuan
Z
Xu
D000970:Antineoplastic Agents; D017209:Apoptosis; D016227:Benzoquinones; D049109:Cell Proliferation; D002470:Cell Survival; D015179:Colorectal Neoplasms; D045325:HCT116 Cells; D006801:Humans; D047029:Lactams, Macrocyclic; D009361:Neoplasm Invasiveness; D009944:Organoplatinum Compounds; D000077150:Oxaliplatin
D000970:Antineoplastic Agents; D016227:Benzoquinones; D047029:Lactams, Macrocyclic; D009944:Organoplatinum Compounds; C448659:17-(dimethylaminoethylamino)-17-demethoxygeldanamycin; D000077150:Oxaliplatin