The effects of some tumor markers on human erythrocyte (HCA-I and HCA-II), bovine erythrocyte (BCA) and bovine lung (CA-IV) carbonic anhydrase enzyme activities in vitro.
OBJECTIVE
The influence of prostatic acid phosphatase (PAP) and human chorionic gonadotropin (HCG), tumor markers have been investigated on human erythrocyte carbonic anhydrase (HCA-I and HCA-II) and bovine erythrocyte (BCA) and bovine lung carbonic anhydrase (CA-IV) in vitro.


BACKGROUND
Tumor markers are substances that can often be detected in higher-than-normal amounts in the blood, urine, or body tissues of some patients with certain types of cancer. Tumor markers are produced either by the tumor itself or by the body in response to the presence of cancer or certain benign (noncancerous) conditions. In addition to their role in cancer diagnosis, some tumor marker levels are measured before treatment to help doctors plan appropriate therapy.


CONCLUSIONS
All of the tumor markers were determined to have inhibition effect, on human CA-I, CA-II, bovine erythrocyte CA (BCA) and bovine lung CA-IV isoenzymes. The effect of each tumor marker on CA was investigated by Wilbur-Andersen method modified by Rickly et al Inhibition effects of two different tumor markers on human CA-I, CA-II, bovine erythrocyte CA (BCA) and bovine lung CA-IV isoenzymes were determined by using the CO2-Hydratase method by plotting activity % vs (tumor markers). I50 values of tumor markers exhibiting inhibition effects were found by means of these graphs (Tab.1, Fig. 2, Ref. 20).
N
N
Demir
H
H
Nadaroglu
A A
AA
Gungor
Y
Y
Demir
D000135:Acid Phosphatase / Q000494:pharmacology*; D000818:Animals; D014408:Biomarkers, Tumor / Q000494:pharmacology*; D024401:Carbonic Anhydrase I / Q000037:antagonists & inhibitors / Q000187:drug effects*; D024402:Carbonic Anhydrase II / Q000037:antagonists & inhibitors / Q000187:drug effects*; D030741:Carbonic Anhydrase IV / Q000037:antagonists & inhibitors / Q000187:drug effects*; D002257:Carbonic Anhydrase Inhibitors / Q000494:pharmacology*; D002256:Carbonic Anhydrases / Q000187:drug effects; D002417:Cattle; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D057075:Enzyme Assays; D004912:Erythrocytes / Q000201:enzymology; D006801:Humans; D066298:In Vitro Techniques; D008168:Lung / Q000201:enzymology
D014408:Biomarkers, Tumor; D002257:Carbonic Anhydrase Inhibitors; D006063:Chorionic Gonadotropin; D000135:Acid Phosphatase; C428439:prostatic acid phosphatase; D024401:Carbonic Anhydrase I; D024402:Carbonic Anhydrase II; D030741:Carbonic Anhydrase IV; D002256:Carbonic Anhydrases
BCA; CA-IV; HCA-I; HCA-II; Zn2+.; carbonic anhydrase; tumor markers