Achieving room temperature DNA amplification by dialling in destabilization.
The ability to amplify nucleic acid biomarkers at room temperature has remained elusive despite the great need of diagnostics suitable for the point of care. To exponentially amplify DNA within a wide range of ambient temperatures (18-26 °C), we explore combining two destabilizing elements in our isothermal lesion-induced DNA amplification system. We demonstrate rapid DNA amplification at the bench without a heat source.
Department of Chemistry, University of Alberta, Edmonton, Alberta T6E 2G2, Canada. gibbsdavis@ualberta.ca.
B Safeenaz
BS
Alladin-Mustan
Catherine J
CJ
Mitran
Julianne M
JM
Gibbs-Davis
D020137:Base Pair Mismatch; D004247:DNA / Q000235:genetics*; D021141:Nucleic Acid Amplification Techniques; D019095:Point-of-Care Systems; D013696:Temperature
D004247:DNA