Optimization of fluoride-containing bioactive glasses as a novel scolicidal agent adjunct to hydatid surgery.
We have presented a novel scolicidal agent made from fluoride-containing bioactive glass (FBG). The samples were characterized by XRD and the ion release behavior of the samples was evaluated at 37°C. The scolicidal activity, cellular response and antibacterial activity and biocompatibility of the different concentrations of the FBG (ranging from 0.5 to 2 mg ml(-1)) were evaluated in vitro and in vivo. The results showed an easy diffusion of the fluoride through the glass matrix in an F concentration-dependent manner. The FBG showed an intensive scolicidal property, so that toxicity effect begun from 5 min and the samples with 20, 10, 5 and 0% fluoride showed 98±2, 93±5.8, 76.2±6 and 5.8±1.7% scolicidal activity, respectively, after 8h exposing time. Our data revealed that scolicidal activity of the FBG increased with the increase of F ratio and concentrations of the bioactive glass. On the other hand, all the synthesized FBG samples found to be cyto-biocompatible when tested in vitro (MTT and LDH assays) and in vivo (no significant infiltration of inflammatory cells compared with control, p>0.05), and antibacterial against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. The results demonstrated that the synthesized FBG might have a potential application in prevention of post-surgery infections, especially hydatidosis.
Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Ali
A
Rostami
Bioengineering Research Group, Nanotechnology and Advanced Materials Department, Materials and Energy Research Center (MERC), P.O. Box 14155-4777, Tehran, Iran.
Masoud
M
Mozafari
Biotechnology Department, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address: mazaher.gholipour@gmail.com.
Mazaher
M
Gholipourmalekabadi
Biologics Research, Biotechnology Center of Excellence, Janssen R&D, LLC, Pharmaceutical Companies of Johnson & Johnson, Spring House, PA 19477, USA; National Biotechnology & Pharmaceutical Association, Chicago, IL 60606, USA.
Hector Hugo
HH
Caicedo
Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Zohreh
Z
Lasjerdi
Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Marzieh
M
Sameni
Department of Medical Biotechnology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran; Department of Tissue Engineering & Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran.
Ali
A
Samadikuchaksaraei
D016475:3T3 Cells; D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology* / Q000627:therapeutic use; D000900:Anti-Bacterial Agents / Q000494:pharmacology / Q000627:therapeutic use; D004443:Echinococcosis / Q000188:drug therapy* / Q000601:surgery; D004446:Echinococcus / Q000187:drug effects*; D004926:Escherichia coli / Q000187:drug effects; D005459:Fluorides / Q000494:pharmacology* / Q000627:therapeutic use; D005898:Glass; D006801:Humans; D051379:Mice; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012008:Recurrence; D012756:Sheep; D013211:Staphylococcus aureus / Q000187:drug effects
D000871:Anthelmintics; D000900:Anti-Bacterial Agents; D005459:Fluorides
Bioactive glass; Fluoride; Hydatidosis; Scolicidal agent; Tissue engineering