Pterostilbene attenuates inflammation in rat heart subjected to ischemia-reperfusion: role of TLR4/NF-κB signaling pathway.
OBJECTIVE
The aim of the present study was to investigate whether pterostilbene could modulate the TLR4/NF-κB signaling, reduce neutrophil accumulation and TNF-α induction in an ischemia/reperfusion injured rat heart model.


METHODS
Rats were randomly exposed to sham operation, myocardial ischemia and reperfusion (MI/R), MI/R + pterostilbene, MI/R + pterostilbene + L-NAME. And myocardial infarct size, apoptosis, TLR4 expression, NF-κB expression, MPO level and TNF-α level were detected.


RESULTS
The results demonstrated that after MI/R, the expressions of myocardial TLR4, NF-κB and caspase-3 increased significantly in ischemia area. Compared with MI/R, pterostilbene significantly attenuated the expressions of TLR4, NF-κB and caspase-3. In addition, it also reduced myeloperoxidase (MPO) levels, both serum and myocardial TNF-α production, myocardial infarct sizes (INF/AAR%) and myocardial apoptosis induced by MI/R. All the effects of pterostilbene were abolished by L-NAME, a nitric oxide synthase inhibitor.


CONCLUSIONS
These data provide evidence that pterostilbene inhibits TLR4/NF-κB signaling and apoptosis in the rat heart subjected to MI/R, which is associated with NO production.
School of Clinical Medicine, Fourth Military Medical University Xi'an 710032, China.
Chen
C
Wang
Department of Cardiovascular Surgery, Qilu Hospital of Shandong University Jinan 250012, China.
Hourong
H
Sun
Department of Cardiovascular Surgery, Qilu Hospital of Shandong University Jinan 250012, China.
Yi
Y
Song
Department of Cardiovascular Surgery, Qilu Hospital of Shandong University Jinan 250012, China.
Zengshan
Z
Ma
Department of Cardiovascular Surgery, Qilu Hospital of Shandong University Jinan 250012, China.
Gong
G
Zhang
Department of Cardiovascular Surgery, Qilu Hospital of Shandong University Jinan 250012, China.
Xinghua
X
Gu
School of Clinical Medicine, Fourth Military Medical University Xi'an 710032, China.
Lei
L
Zhao
Pterostilbene; TLR4/NF-κB signaling; TNF-α; apoptosis; ischemia/reperfusion injury; neutrophil