Immune-enhancing activity of polysaccharides isolated from Atractylodis macrocephalae Koidz.
This study evaluated the immune-enhancing activity of polysaccharides from the rhizoma of Atractylodis macrocephalae Koidz (RAMPS) in vitro. Lymphocyte proliferation, cell cycle distribution, and percentages of CD4(+) and CD8(+) cells were determined. Different concentrations of RAMPS were added to peripheral blood T lymphocytes. Results showed that RAMPStp and RAMPS60c could significantly enhance T lymphocyte proliferation individually or synergistically with phytohemagglutinin at most concentrations. The active sites of RAMPStp and RAMPS60c were then selected. Lymphocyte cell cycle distribution and percentages of CD4(+) and CD8(+) cells were determined by flow cytometry. At most time points, RAMPS60c and RAMPStp could promote lymphocytes enter into S and G2/M phases. RAMPStp and RAMPS60c effectively improved the percentages of CD4(+) and CD8(+) T cells. RAMPStp produced optimal effects. Therefore, RAMPStp could be used as a component of novel immunopotentiators.
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, PR China.
Wenjing
W
Sun
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, PR China.
Kai
K
Meng
Central Hospital of Tai'an City, Tai'an, Shandong 271018, PR China.
Chunhua
C
Qi
Central Hospital of Tai'an City, Tai'an, Shandong 271018, PR China.
Xianyong
X
Yang
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, PR China.
Yuge
Y
Wang
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, PR China.
Wentao
W
Fan
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, PR China.
Zhengui
Z
Yan
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, PR China. Electronic address: zhaoxn@sdau.edu.cn.
Xiaona
X
Zhao
Research Center for Animal Disease Control Engineering Shandong Province, Tai'an 271018, PR China. Electronic address: liujz@sdau.edu.cn.
Jianzhu
J
Liu
D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D031174:Atractylodes / Q000737:chemistry*; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D027343:Rhizome / Q000737:chemistry*
D000276:Adjuvants, Immunologic; D004365:Drugs, Chinese Herbal; D011134:Polysaccharides
Atractylodis macrocephalae Koidz; Cell cycle; Immune-enhancing; Lymphocyte proliferation; Polysaccharides