Sanitizing Effect of Ethanol Against Biofilms Formed by Three Gram-Negative Pathogenic Bacteria.
Sanitizing effect of ethanol on a Yersinia enterocolitica biofilm was evaluated in terms of biomass removal and bactericidal activity. We found that 40 % ethanol was most effective for biofilm biomass removal; however, no significant difference was observed in bactericidal activity between treatment with 40 and 70 % ethanol. This unexpected low ethanol concentration requirement for biomass removal was confirmed using biofilms of two additional pathogenic bacteria, Aeromonas hydrophila and Xanthomonas oryzae. Although only three pathogenic Gram-negative bacteria were tested and the biofilm in nature was different from the biofilm in this study, the results in this study suggested the possible re-evaluation of the effective sanitizing ethanol concentration 70 %, which is the concentration commonly employed for sanitization, on bacteria in a biofilm.
Department of Forest Products and Biotechnology, College of Forest Science, Kookmin University, 77 Jeongneung-ro, Seongbuk-gu, Seoul, 136-702, Korea.
Han-Saem
HS
Park
Youngseok
Y
Ham
Keum
K
Shin
Yeong-Suk
YS
Kim
Tae-Jong
TJ
Kim
D016980:Aeromonas hydrophila / Q000187:drug effects / Q000502:physiology*; D018441:Biofilms / Q000187:drug effects*; D004202:Disinfectants / Q000494:pharmacology*; D000431:Ethanol / Q000494:pharmacology*; D050296:Microbial Viability / Q000187:drug effects*; D014974:Xanthomonas / Q000187:drug effects / Q000502:physiology*; D015008:Yersinia enterocolitica / Q000187:drug effects / Q000502:physiology*
D004202:Disinfectants; D000431:Ethanol