The relative values of CD8+CD25+Foxp3brigh Treg cells correlate with selected lung function parameters in asthma.
The study aimed to detect CD8(+)CD25(+)FoxP3(brigh) Tregs and investigate their possible association with selected lung function values. CD8(+)CD25(+)FoxP3(brigh) Tregs were detected by flow cytometry in the peripheral blood of 25 patients with severe asthma (SA), 25 patients with mild-to-moderate asthma (MA), and 25 age-matched healthy donors (NC). The percentages of CD8(+)CD25(+)FoxP3(brigh) Tregs of the patients with severe (3.4 ± 4.55), and mild-to-moderate asthma (7.5 ± 8.15), were markedly lower than those of controls (12.1 ± 13.2). The mean forced expiratory volume in 1 s (FEV1) % predicted value in severe asthma subpopulation was significantly lower (67.05 ± 15.98%) when compared with that of mild-to-moderate asthma subgroup (87.71 ± 16.12%). Interestingly, the percentages of CD8(+)CD25(+)FoxP3(brigh) Tregs correlate with mean peak expiratory flow (PEF)% predicted values in severe (r = 0.7, P <0.01) and mild-to-moderate (r = 0.73, P <0.01) asthma. In contrast, this parameter was positively correlated with FEV1% predicted values in the severe asthmatics only (r = 0.71, P <0.01). In summary, this study establishes a link between the percentage of CD8(+)CD25(+)FoxP3(brigh) Tregs and selected lung function parameters, suggesting that this parameter has potential as a marker for inflammation and airflow obstruction.
Department of Laboratory Diagnostics, II Chair of Internal Medicine, Medical University of Lodz, Poland eusemak@yahoo.com.
M
M
Eusebio
Department of Internal Medicine, II Chair of Internal Medicine, Medical University of Lodz, Poland.
P
P
Kuna
Department of Laboratory Diagnostics, II Chair of Internal Medicine, Medical University of Lodz, Poland.
L
L
Kraszula
Department of Internal Medicine, II Chair of Internal Medicine, Medical University of Lodz, Poland.
M
M
Kupczyk
Department of Laboratory Diagnostics, II Chair of Internal Medicine, Medical University of Lodz, Poland.
M
M
Pietruczuk
D000328:Adult; D001249:Asthma / Q000503:physiopathology*; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism / Q000473:pathology*; D016022:Case-Control Studies; D005260:Female; D005541:Forced Expiratory Volume / Q000502:physiology; D051858:Forkhead Transcription Factors / Q000378:metabolism*; D006801:Humans; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism*; D008168:Lung / Q000503:physiopathology*; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D050378:T-Lymphocytes, Regulatory / Q000378:metabolism / Q000473:pathology*
C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; C508017:IL2RA protein, human; D053645:Interleukin-2 Receptor alpha Subunit
FEV1; PEF; Treg cells; asthma; marker