Associations between CLCNKA_B tag SNPs with essential hypertension and interactions between genetic and environmental factors in an island population in China.
OBJECTIVE
Essential hypertension (EH) is known as the result from the interaction of environmental and genetic factors. We selected tag SNPs of CLCNKA_B genes as gene markers, to elucidate the relationship between CLCNKA_B and EH, and to determine the possible interaction among tag SNPs and dietary factors in island of China.


METHODS
A case-control study was conducted in Changshan islands of China, blood samples of 806 participants were genotyped, and the general characteristics and dietary habits of them were collected. Unconditional logistic regression (ULR) was used to assess the effects of CLCNKA_B tag SNPs and dietary factors on EH. Generalized multifactor dimensionality reduction (GMDR) was used to test gene-environment interaction for EH risk.


RESULTS
Four SNPs were identified as the tag SNPs of CLCNKA_B. Recessive model for rs5253 and rs2275166 were marginal associated with the decrease risk of EH (OR = 0.36, 95% CI = 0.12-1.07 for rs5253; OR = 0.40, 95% CI = 0.16-1.05 for rs2275166). In GMDR, the five-factor interaction model of rs1010069, salt, marine products, meat and edible oil consumption was the best model, with a maximum CVC of 10/10 and a TBA of 0.638 (p = 0.001). In ULR, compared with subjects carried wild genotypes and null dietary risk factor, those with three or more dietary risk factors and mutation genotypes had 5.90-fold EH risk (95% CI:2.24-15.53).


CONCLUSIONS
Though the single loci of tag SNPs of CLCNKA_B are not enough to significantly increase the EH susceptibility, the combination of CLCNKA tag SNP, salt, marine products, meat and edible oil consumption is associated with elevated risk.
a Department of Clinical Epidemiology , Institute of Cardiovascular Diseases, and Center of Evidence Based Medicine, the First Affiliated Hospital, China Medical University , Shenyang , China .
Xin
X
Chen
a Department of Clinical Epidemiology , Institute of Cardiovascular Diseases, and Center of Evidence Based Medicine, the First Affiliated Hospital, China Medical University , Shenyang , China .
Bo
B
Zhou
a Department of Clinical Epidemiology , Institute of Cardiovascular Diseases, and Center of Evidence Based Medicine, the First Affiliated Hospital, China Medical University , Shenyang , China .
Xiaowen
X
Hou
b School of Forensic Medicine, China Medical University , Shenyang , China , and.
Jiaxin
J
Xing
c Department of Rehabilitation , Central Hospital of Dalian Municipal , Dalian , China.
Safeng
S
Zou
a Department of Clinical Epidemiology , Institute of Cardiovascular Diseases, and Center of Evidence Based Medicine, the First Affiliated Hospital, China Medical University , Shenyang , China .
Xiaomei
X
Wu
a Department of Clinical Epidemiology , Institute of Cardiovascular Diseases, and Center of Evidence Based Medicine, the First Affiliated Hospital, China Medical University , Shenyang , China .
Bing
B
Ma
a Department of Clinical Epidemiology , Institute of Cardiovascular Diseases, and Center of Evidence Based Medicine, the First Affiliated Hospital, China Medical University , Shenyang , China .
Jingpu
J
Shi
D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D018118:Chloride Channels / Q000235:genetics*; D000075222:Essential Hypertension; D005247:Feeding Behavior; D005260:Female; D059647:Gene-Environment Interaction; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D006801:Humans; D006973:Hypertension / Q000208:ethnology / Q000235:genetics / Q000503:physiopathology; D016015:Logistic Models; D008297:Male; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors
C489932:CLCNKA protein, human; C412733:CLCNKB protein, human; D018118:Chloride Channels; D005819:Genetic Markers
CLCNKA; CLCNKB; Essential hypertension; gene–environmental interaction; tag SNP