Chrysin-organogermanium (IV) complex induced Colo205 cell apoptosis-associated mitochondrial function and anti-angiogenesis.
Colorectal cancer, a kind of malignant cancer, has more than 1 million new patients and results in 0.5 million deaths every year globally based on the estimation of Globocan in 2008. One of the most important issues against colon cancer is tumor metastasis. Anti-angiogenesis, a form of targeted therapy uses drugs or other substances to prevent the new blood vessel formation, which is critical for tumor metastasis. In our previous studies, we have demonstrated a simple method to synthesize Chry-Ge complex through the reaction between chrysin and triphenylgermanium bromide. In this work, we investigated the mechanism of Chry-Ge induced Colo205 cell apoptosis. We found that Chry-Ge could induce apoptosis in Colo205 cells in mitochondrial-dependent pathway, cause the reorganization of cytoskeleton and induce the damage of nucleus in Colo205 cells. Besides, Chry-Ge was also found to induce membrane ultrastructural changes in Colo205 cells by AFM. Further, we found that Chry-Ge can inhibit tube formation of human umbilical vascular endothelial cell in vitro. Chry-Ge was also tested in vivo in the chicken chorioallantoic membrane (CAM) assay and found to inhibit bFGF-treated CAMs development. These results suggested that Chry-Ge could induce Colo205 cell apoptosis by mitochondrial pathway and anti-angiogenesis, highlighting the use of organic germanium agents for the treatment of colorectal cancer.
Department of Chemistry of Jinan University, Guangzhou, China.|State Key Laboratory of Quality Research in Chinese Medicines, Macau University of Science and Technology, Macau, China.
Fen
F
Yang
Department of Bioengineering, Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan University, Guangzhou, China.
Longcai
L
Gong
Department of Chemistry of Jinan University, Guangzhou, China.|State Key Laboratory of Quality Research in Chinese Medicines, Macau University of Science and Technology, Macau, China.
Hua
H
Jin
Department of Chemistry of Jinan University, Guangzhou, China.|State Key Laboratory of Quality Research in Chinese Medicines, Macau University of Science and Technology, Macau, China.
Jiang
J
Pi
Department of Chemistry of Jinan University, Guangzhou, China.
Haihua
H
Bai
Department of Bioengineering, Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan University, Guangzhou, China.
Hong
H
Wang
Department of Chemistry of Jinan University, Guangzhou, China.
Huaihong
H
Cai
Department of Chemistry of Jinan University, Guangzhou, China.
Peihui
P
Yang
Department of Chemistry of Jinan University, Guangzhou, China.|State Key Laboratory of Quality Research in Chinese Medicines, Macau University of Science and Technology, Macau, China.
Jiye
J
Cai
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002645:Chickens; D049033:Chorioallantoic Membrane / Q000187:drug effects; D003106:Colon; D042783:Endothelial Cells / Q000187:drug effects / Q000502:physiology; D005419:Flavonoids / Q000494:pharmacology*; D005857:Germanium / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008928:Mitochondria / Q000187:drug effects*; D018919:Neovascularization, Physiologic / Q000187:drug effects; D009942:Organometallic Compounds / Q000494:pharmacology*
D005419:Flavonoids; D009942:Organometallic Compounds; D005857:Germanium; C043561:chrysin
Chry-Ge; Chrysin; Colo205; atomic force microscopy (AFM); laser scanning confocal microscope (LSCM)