Effect of chemical stabilizers on the thermostability and infectivity of a representative panel of freeze dried viruses.
As a partner of the European Virus Archive (EVA) FP7 project, our laboratory maintains a large collection of freeze-dried viruses. The distribution of these viruses to academic researchers, public health organizations and industry is one major aim of the EVA consortium. It is known that lyophilization requires appropriate stabilizers to prevent inactivation of the virus. However, few studies have investigated the influence of different stabilizers and lyophilization protocols on the thermostability of different viruses. In order to identify optimal lyophilization conditions that will deliver maximum retention of viral infectivity titre, different stabilizer formulations containing trehalose, sorbitol, sucrose or foetal bovine serum were evaluated for their efficacy in stabilizing a representative panel of freeze dried viruses at different storage temperatures (-20°C, +4°C and +20°C) for one week, the two latter mimicking suboptimal shipping conditions. The Tissue Culture Infectious Dose 50% (TCID50) assay was used to compare the titres of infectious virus. The results obtained using four relevant and model viruses (enveloped/non enveloped RNA/DNA viruses) still serve to improve the freeze drying conditions needed for the development and the distribution of a large virus collection.
Aix Marseille Université, IRD French Institute of Research for Development, EHESP French School of Public Health, EPV UMR_D 190 "Emergence des Pathologies Virales", & IHU Institute hospitalo-universitaire Méditerranée Infection, APHM Public Hospitals of Marseille 13385, Marseille, France.
Boris
B
Pastorino
Aix Marseille Université, IRD French Institute of Research for Development, EHESP French School of Public Health, EPV UMR_D 190 "Emergence des Pathologies Virales", & IHU Institute hospitalo-universitaire Méditerranée Infection, APHM Public Hospitals of Marseille 13385, Marseille, France.
Cecile
C
Baronti
Aix Marseille Université, IRD French Institute of Research for Development, EHESP French School of Public Health, EPV UMR_D 190 "Emergence des Pathologies Virales", & IHU Institute hospitalo-universitaire Méditerranée Infection, APHM Public Hospitals of Marseille 13385, Marseille, France.
Ernest A
EA
Gould
Aix Marseille Université, IRD French Institute of Research for Development, EHESP French School of Public Health, EPV UMR_D 190 "Emergence des Pathologies Virales", & IHU Institute hospitalo-universitaire Méditerranée Infection, APHM Public Hospitals of Marseille 13385, Marseille, France.
Remi N
RN
Charrel
Aix Marseille Université, IRD French Institute of Research for Development, EHESP French School of Public Health, EPV UMR_D 190 "Emergence des Pathologies Virales", & IHU Institute hospitalo-universitaire Méditerranée Infection, APHM Public Hospitals of Marseille 13385, Marseille, France.
Xavier
X
de Lamballerie
D002460:Cell Line; D005079:Excipients / Q000494:pharmacology*; D005612:Freeze Drying / Q000379:methods*; D006801:Humans; D012328:RNA Viruses / Q000187:drug effects / Q000472:pathogenicity*; D013696:Temperature
D005079:Excipients