A triple-color fluorescent probe for multiple nuclease assays.
We develop a triple-color fluorescent probe which may function as a lab-on-a-DNA-molecule for simultaneous detection of multiple exonucleases/restriction endonucleases. This triple-color fluorescent probe can be further applied for the discrimination of seven exonucleases and four cell lines as well as the screening of various nuclease inhibitors.
Single-Molecule Detection and Imaging Laboratory, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China. zhangcy@siat.ac.cn.
Qinfeng
Q
Xu
Yihong
Y
Zhang
Chun-yang
CY
Zhang
D015075:2-Aminopurine / Q000737:chemistry; D045744:Cell Line, Tumor; D015342:DNA Probes / Q000737:chemistry* / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000737:chemistry* / Q000378:metabolism; D005092:Exonucleases / Q000737:chemistry* / Q000378:metabolism; D019793:Fluorescein / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D012235:Rhodamines / Q000737:chemistry
C114557:5-carboxytetramethylrhodamine succinimidyl ester; D015342:DNA Probes; D005456:Fluorescent Dyes; D012235:Rhodamines; D015075:2-Aminopurine; D005092:Exonucleases; D004262:DNA Restriction Enzymes; D019793:Fluorescein