Imaging the lipid bilayer of giant unilamellar vesicles using red-to-blue light upconversion.
Red-to-blue triplet-triplet annihilation upconversion was obtained in giant unilamellar vesicles. The upconverted light was homogeneously distributed across the membrane and could be utilized for the imaging of individual giant vesicles in three dimensions. These results show the great potential of TTA-UC for imaging applications under anoxic conditions.
Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2333 CC Leiden, The Netherlands. bonnet@chem.leidenuniv.nl.
Sven H C
SH
Askes
Néstor
N
López Mora
Rolf
R
Harkes
Roman I
RI
Koning
Bram
B
Koster
Thomas
T
Schmidt
Alexander
A
Kros
Sylvestre
S
Bonnet
D008051:Lipid Bilayers / Q000737:chemistry*; D008853:Microscopy; D010165:Palladium / Q000737:chemistry; D010569:Perylene / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry; D010714:Phosphatidylethanolamines / Q000737:chemistry; D017319:Photosensitizing Agents / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011166:Porphyrins / Q000737:chemistry; D053835:Unilamellar Liposomes / Q000737:chemistry*
D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D017319:Photosensitizing Agents; D011166:Porphyrins; D053835:Unilamellar Liposomes; C038089:1,2-distearoylphosphatidylethanolamine; D011092:Polyethylene Glycols; D010569:Perylene; D010165:Palladium