Dynamics of statistically confident particle sizes and concentrations in blood plasma obtained by the dynamic light scattering method.
The work is devoted to the study of sizes and concentrations of proteins, and their aggregates in blood plasma samples, using static and dynamic light scattering methods. A new approach is proposed based on multiple repetition of measurements of intensity size distribution and on counting the number of registrations of different sizes, which made it possible to obtain statistically confident particle sizes and concentrations in the blood plasma. It was revealed that statistically confident particle sizes in the blood plasma were stable during 30 h of observations, whereas the concentrations of particles of different sizes varied as a result of redistribution of material between them owing to the protein degradation processes.
Lebedev Physical Institute of the Russian Academy of Sciences, G.S. Landsberg Optical Department, Laboratory for Nonlinear Optics and Light Scattering, 53 Leninskij Prospekt, Moscow 119991, RussiabNational Research Nuclear University "MEPhI", 31 Kashirsko.
Leonid L
LL
Chaikov
Lebedev Physical Institute of the Russian Academy of Sciences, G.S. Landsberg Optical Department, Laboratory for Nonlinear Optics and Light Scattering, 53 Leninskij Prospekt, Moscow 119991, Russia.
Marina N
MN
Kirichenko
Lebedev Physical Institute of the Russian Academy of Sciences, G.S. Landsberg Optical Department, Laboratory for Nonlinear Optics and Light Scattering, 53 Leninskij Prospekt, Moscow 119991, Russia.
Svetlana V
SV
Krivokhizha
Lebedev Physical Institute of the Russian Academy of Sciences, G.S. Landsberg Optical Department, Laboratory for Nonlinear Optics and Light Scattering, 53 Leninskij Prospekt, Moscow 119991, Russia.
Alexander R
AR
Zaritskiy
D000465:Algorithms; D001798:Blood Proteins / Q000032:analysis / Q000737:chemistry* / Q000648:ultrastructure*; D055252:Cell-Derived Microparticles / Q000737:chemistry* / Q000648:ultrastructure*; D003198:Computer Simulation; D000067493:Dynamic Light Scattering / Q000379:methods*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D008955:Models, Cardiovascular; D015233:Models, Statistical; D010316:Particle Size; D010949:Plasma / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D001798:Blood Proteins