Expression optimization and biochemical properties of two glycosyl hydrolase family 3 beta-glucosidases.
The β-glucosidases from Saccharomycopsis fibuligera (SfBGL1) and Trichoderma reesei (TrBGL1) were cloned and expressed in Pichia pastoris. Methanol concentration and pH significantly affected the production. The combined effects of the two factors were optimized by using the response surface method, resulting in a 137% and 84% increase in rTrBGL1 and rSfBGL1 yield compared to single-factor experiment. Structure and biochemical properties of the two enzyme were investigated and compared. They belong to glycosyl hydrolase family 3 and exhibit significant hydrolysis activity and low-level transglycosylation activity. The two enzymes show similar substrate affinity and ion-tolerance, and both of them can be activated by Cr(6+), Mn(2+) and Fe(2+). The rSfBGL1 has greater catalytic speed, higher specific activity and acid-tolerance than rTrBGL1, but rTrBGL1 is more thermostable and has higher optimal temperature than rSfBGL1. This study provides a useful and quick optimal method for recombinant enzyme production and makes a valuable comparison of biochemical properties, which opens important avenues of exploration for relationship between structure and function and further practical applications.
R&D Center for Petrochemical Technology, Tianjin University, Tianjin 300072, China; Collaborative Innovation Center of Chemical Science and Engineering, Tianjin 300072, China; Key Laboratory for Green Chemical Technology of Ministry of Education, R&D Center for Petrochemical Technology, Tianjin University, Tianjin 300072, China. Electronic address: myy@tju.edu.cn.
Yuanyuan
Y
Ma
R&D Center for Petrochemical Technology, Tianjin University, Tianjin 300072, China; Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China. Electronic address: lxw_2010@126.com.
Xuewei
X
Liu
R&D Center for Petrochemical Technology, Tianjin University, Tianjin 300072, China; Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China. Electronic address: tjuirisyin@163.com.
Yanchen
Y
Yin
R&D Center for Petrochemical Technology, Tianjin University, Tianjin 300072, China; Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China. Electronic address: zouchaotju@163.com.
Chao
C
Zou
R&D Center for Petrochemical Technology, Tianjin University, Tianjin 300072, China; Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China. Electronic address: wangwanchao31@126.com.
Wanchao
W
Wang
R&D Center for Petrochemical Technology, Tianjin University, Tianjin 300072, China; Collaborative Innovation Center of Chemical Science and Engineering, Tianjin 300072, China; Key Laboratory for Green Chemical Technology of Ministry of Education, R&D Center for Petrochemical Technology, Tianjin University, Tianjin 300072, China. Electronic address: slzhou@tju.edu.cn.
Shaolan
S
Zou
R&D Center for Petrochemical Technology, Tianjin University, Tianjin 300072, China. Electronic address: hjf@tju.edu.cn.
Jiefang
J
Hong
R&D Center for Petrochemical Technology, Tianjin University, Tianjin 300072, China; Collaborative Innovation Center of Chemical Science and Engineering, Tianjin 300072, China; Key Laboratory for Green Chemical Technology of Ministry of Education, R&D Center for Petrochemical Technology, Tianjin University, Tianjin 300072, China. Electronic address: mhzhangtju@gmail.com.
Minhua
M
Zhang
D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D006867:Hydrolases; D008969:Molecular Sequence Data; D010843:Pichia / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012442:Saccharomycopsis / Q000201:enzymology / Q000235:genetics; D014242:Trichoderma / Q000201:enzymology / Q000235:genetics; D001617:beta-Glucosidase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
D005656:Fungal Proteins; D011994:Recombinant Proteins; D006867:Hydrolases; D001617:beta-Glucosidase
Biochemical property; Pichia pastoris; Response surface method; Saccharomycopsis fibuligera; Trichoderma reesei; β-Glucosidase