Antibacterial and Anti-inflammatory Activities of Ppc-1, Active Principle of the Cellular Slime Mold Polysphondylium pseudo-candidum.
The diisopentenyloxy quinolobactin derivative 3-methylbut-2-enyl-4-methoxy-8-[(3-methylbut-2-enyl)oxy] quinoline-2-carboxylate, also named as Ppc-1, has been initially isolated from the fruiting bodies of the cellular slime mold Polysphondylium pseudo-candidum. Given that few data are available in the literature concerning the biological properties of this compound, this study was undertaken to evaluate its antibacterial and anti-inflammatory properties. Ppc-1 exerted antibacterial activity on the Gram negative periodontopathogen Porphyromonas gingivalis, while it had no such effect on the other bacterial species tested. The antibacterial activity of Ppc-1 appeared to result from its ability to permeate the cell membrane. Using the U937-3xκB-LUC human monocytic cell line, Ppc-1 was found to dose-dependently inhibit the lipopolysaccharide-induced NF-κB activation, a signaling pathway that has been associated with inflammatory mediator secretion. In conclusion, Ppc-1, by exhibiting a dual mode of action including antibacterial and anti-inflammatory activities, may represent a promising targeted therapeutic agent for periodontal diseases.
Jabrane
J
Azelmat
Serena
S
Fiorito
Salvatore
S
Genovese
Francesco
F
Epifano
Groupe de Recherche en Ecologie Buccale (GREB), Faculte de Medecine Dentaire, Universite Laval, 2420 Rue de la Terrasse, Quebec City, Quebec, G1V 0A6, Canada. Daniel.Grenier@greb.ulaval.ca.
Daniel
D
Grenier
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D016837:Dictyosteliida / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D045782:Hemiterpenes / Q000494:pharmacology*; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D016328:NF-kappa B / Q000378:metabolism; D011804:Quinolines / Q000494:pharmacology*
C000609069:3-methylbut-2-enyl-4-methoxy-8-((3- methylbut-2-enyl)oxy)quinoline-2-carboxylate; D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; D045782:Hemiterpenes; D008070:Lipopolysaccharides; D016328:NF-kappa B; D011804:Quinolines