A specific process to purify 2-methyl-D-erythritol-4-phosphate enzymatically converted from D-glyceraldehyde-3-phosphate and pyruvate.
A one-pot enzymatic cascade was established to synthesize MEP, one of the key intermediates in the MEP terpenoid biosynthetic pathway. D-GAP and sodium pyruvate were converted to MEP in a reaction catalyzed by DXP synthase and DXP reductoisomerase (DXR) in the presence of the coenzymes ThPP, NADPH, and Mg2+. The product was then isolated by using a specific two-step purification process and MEP was obtained in a yield of nearly 60% and high purity. Importantly, MEP prepared by this way was totally free from contamination by minor amounts of DXP that was not completely convertible by DXR.
Shao-Qing
SQ
Yang
Jian
J
Deng
Qian-Qian
QQ
Wu
Heng
H
Li
Wen-Yun
WY
Gao
D004896:Erythritol / Q000031:analogs & derivatives* / Q000737:chemistry*; D005986:Glyceraldehyde 3-Phosphate / Q000737:chemistry*; D015394:Molecular Structure; D019289:Pyruvic Acid / Q000737:chemistry*; D013729:Terpenes / Q000737:chemistry / Q000378:metabolism
C000598497:2-methyl-D-erythritol-4-phosphate; D013729:Terpenes; D005986:Glyceraldehyde 3-Phosphate; D019289:Pyruvic Acid; D004896:Erythritol