Single-Nucleotide Polymorphisms in Pig EPHX1 Gene are Associated with Pork Quality Traits.
Epoxide hydrolase 1 (EPHX1) plays an important role in both the activation and detoxification of exogenous chemicals. Reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that the highest level of EPHX1 expression occurred in Berkshire liver, which is an organ that plays a key role in detoxification. We examined EPHX1 SNPs to analyze effect on increased expression of EPHX1 gene in Berkshire liver by total of 192 pigs of a pure Berkshire line (males = 97; females = 95). As a result, two nonsynonymous SNPs (nsSNPs) of EPHX1 were found from c.685T>G and c.776C > T, and located in 5th and 6th exons, respectively, which constitute the A/b hydrolase 1 domain of epoxide hydrolase. The nsSNP c.685T > G was significant differences in meat color, protein content, collagen content, and pH24 hr. Especially, T and G alleles of the nsSNP c.685T > G were significantly associated with CIE a*/CIE b* and protein content/pH24 hr, respectively. The nsSNP c.776C > T was significant differences in drip loss and protein content. Among meat quality traits to associate with SNPs, the protein content was only significantly associated with sex. Therefore, it is suggested that nsSNP c.685T > G in EPHX1 gene is a potential to apply as appropriate DNA markers for improvement of porcine economic traits.
a Swine Science and Technology Center , Gyeongnam National University of Science & Technology , Jinju , South Korea.
Hwak Rae
HR
Cho
Jeongim
J
Ha
Seul Gi
SG
Kwon
Jung Hye
JH
Hwang
Da Hye
DH
Park
Tae Wan
TW
Kim
Hak-Kyo
HK
Lee
Ki-Duk
KD
Song
Sam Woong
SW
Kim
Chul Wook
CW
Kim
D000818:Animals; D004851:Epoxide Hydrolases / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D005260:Female; D008099:Liver / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism; D008297:Male; D008460:Meat / Q000592:standards*; D009928:Organ Specificity; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D056910:Republic of Korea; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine / Q000235:genetics*
D004851:Epoxide Hydrolases
A/b hydrolase 1 domain; Epoxide hydrolase 1; Exon; RT-PCR; Sex; nsSNPS