Preparation and characterization of cross-linked carboxymethyl chitin porous membrane scaffold for biomedical applications.
Porous dermal scaffold membrane (PDSM) was successfully prepared by using a so-called sol-gel freeze-drying method. In this method, the carboxymethyl chitin (CMC) hydrosol was first cross-linked by 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS), and then lyophilized to form the PDSM. For the first time, this research focused on the cross-linked CMC as the only component for three-dimensional PDSM. The effects of cross-linking conditions on the performance of the PDSM were investigated. And PDSM with optimal performance was obtained through 4-h cross-linking at 4 wt% of CMC concentration in the hydrosol, where the mass ratio of EDC to NHS to CMC was 5:3:10. The porosity of the optimized PDSM was more than 90% and the water swelling rate was above 4000%. The pore size was well distributed and was between 100 μm and 200 μm. And the tensile strength was above 0.09 MPa. The as-made PDSM could be degraded above 80% in 12 days in the presence of a 0.2mg/mL lysozyme solution. Very importantly, the PDSM had no cytotoxicity and good biocompatibility from MTT assays. Our results showed the application possibility of the as-prepared PDSM as dermal scaffold for skin tissue engineering.
College of Chemistry and Chemical Engineering, Shenzhen University, Shenzhen, Guangdong 518060, China. Electronic address: snowyzlj@163.com.
Liqing
L
Zhao
College of Chemistry and Chemical Engineering, Shenzhen University, Shenzhen, Guangdong 518060, China. Electronic address: yebsd@sina.com.
Yiguang
Y
Wu
College of Chemistry and Chemical Engineering, Shenzhen University, Shenzhen, Guangdong 518060, China.
Shu
S
Chen
College of Chemistry and Chemical Engineering, Shenzhen University, Shenzhen, Guangdong 518060, China.
Tao
T
Xing
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism; D002234:Carbodiimides / Q000737:chemistry / Q000378:metabolism; D002460:Cell Line; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D003432:Cross-Linking Reagents / Q000737:chemistry* / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology; D005612:Freeze Drying; D008422:Materials Testing; D051379:Mice; D016062:Porosity; D013388:Succinimides / Q000737:chemistry / Q000378:metabolism; D013718:Tensile Strength; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
D001672:Biocompatible Materials; D002234:Carbodiimides; D003432:Cross-Linking Reagents; D013388:Succinimides; D002686:Chitin; C044872:O-(carboxymethyl)chitin; C001426:N-hydroxysuccinimide
Biodegradability; Cross-linked carboxymethyl chitin; Sol–gel freeze-drying method; Tissue engineering dermal scaffold