Efficacy of a combined inactivated porcine reproductive and respiratory syndrome virus vaccine using North American and European strains in specific pathogen free pigs.
In Korea, porcine reproductive and respiratory syndrome (PRRS) is caused by European (type 1) and North American (type 2) strains of PRRS virus (PRRSV). In the present study, the efficacy of a multi-strain PRRSV vaccine inactivated with binary ethylenimine (BEI) was evaluated in pigs. The vaccine contained one type 1 strain (GCEU0907) and two type 2 strains (GC4019 and GC6262). Three vaccinated groups (four pigs per group) and three mock vaccinated groups (four pigs per group) were challenged with infectious PRRSV (strains GC4019, GC6262 or GCEU0907), then euthanased at 28 days post-infection. Mean anti-PRRSV neutralising antibody titres were significantly higher in the vaccinated groups than in the mock vaccinated groups. Mean blood virus titres in the mock vaccinated groups were significantly higher than those in the vaccinated groups from 5 to 28 days post-infection. On pathological examination, there were less severe macroscopic and microscopic lesions in vaccinated pigs compared with mock vaccinated pigs.
Viral Infectious Disease Research Centre, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Republic of Korea; Department of Pharmacy, College of Pharmacy, Korea University, Sejong, South Korea.
Minjoo
M
Yeom
Korea Zoonosis Research Institute, Chonbuk National University, Iksan, Jeonbuk 561-756, Republic of Korea.
Kwang-Soo
KS
Lyoo
Research Unit, Green Cross Veterinary Products, Yong-in 449-903, Republic of Korea.
Bo-Kyu
BK
Kang
Viral Infectious Disease Research Centre, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Republic of Korea; Department of Pharmacy, College of Pharmacy, Korea University, Sejong, South Korea. Electronic address: sds1@kribb.re.kr.
Daesub
D
Song
Department of Veterinary Medicine Virology Laboratory, College of Veterinary Medicine, Seoul National University, Kwanak-gu, Seoul 151-742, Republic of Korea.
Bongkyun
B
Park
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D009500:Neutralization Tests / Q000662:veterinary; D019318:Porcine Reproductive and Respiratory Syndrome / Q000517:prevention & control*; D019316:Porcine respiratory and reproductive syndrome virus / Q000145:classification / Q000235:genetics*; D013047:Specific Pathogen-Free Organisms; D013552:Swine; D014765:Viral Vaccines / Q000009:adverse effects / Q000276:immunology*; D014766:Viremia
D014765:Viral Vaccines
Combined inactivated vaccine; Porcine reproductive and respiratory syndrome virus