Effect of Selenium Supplementation on Apoptosis and Cell Cycle Blockage of Renal Cells in Broilers Fed a Diet Containing Aflatoxin B1.
The aim of the study was to investigate the competency of selenium (Se) in counteracting the adverse effects of aflatoxin B1 (AFB1) on apoptosis, cell cycle, and proliferation of nephritic cells. Two hundred forty 1-day-old healthy male avian broilers were randomly divided into four groups and fed basal diet (control group), 0.3 mg/kg AFB1 diet (AFB1 group), 0.4 mg/kg Se diet (+Se group), and 0.3 mg/kg AFB1 + 0.4 mg/kg Se diet (AFB1 + Se group), respectively. Compared to the control group, the number of apoptotic renal cells and expressions of Bax and caspase-3 messenger RNA (mRNA) were significantly increased, while the expression of Bcl-2 was significantly decreased in the AFB1 and the +Se groups (p < 0.01). A significantly decreased proliferating cell nuclear antigen (PCNA) expression and arrested G0/G1 phases of the cell cycle were also seen in the AFB1 and the +Se groups when compared with those of the control group. Moreover, these parameters were restored to the control group levels in the AFB1 + Se group. These results suggested that sodium selenite supplied in the diet could effectively inhibit AFB1-induced apoptosis and cell cycle blockage in renal cells of broiler.
Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya' an, 625014, Sichuan, People's Republic of China.
Zhengqiang
Z
Yu
Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya' an, 625014, Sichuan, People's Republic of China.
Fengyuan
F
Wang
Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya' an, 625014, Sichuan, People's Republic of China.
Na
N
Liang
Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya' an, 625014, Sichuan, People's Republic of China.
Chuhan
C
Wang
Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya' an, 625014, Sichuan, People's Republic of China. pengxi197313@163.com.
Xi
X
Peng
Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya' an, 625014, Sichuan, People's Republic of China.
Jing
J
Fang
Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya' an, 625014, Sichuan, People's Republic of China.
Hengmin
H
Cui
Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya' an, 625014, Sichuan, People's Republic of China.
Muhammad
M
Jameel Mughal
Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya' an, 625014, Sichuan, People's Republic of China.
Weimin
W
Lai
D016604:Aflatoxin B1 / Q000037:antagonists & inhibitors / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002273:Carcinogens / Q000633:toxicity*; D053148:Caspase 3 / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects*; D002645:Chickens; D019938:Cyclin D1 / Q000378:metabolism; D019587:Dietary Supplements; D007668:Kidney / Q000166:cytology* / Q000187:drug effects; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis; D018036:Selenium Compounds / Q000494:pharmacology*; D051028:bcl-2-Associated X Protein / Q000378:metabolism
D002273:Carcinogens; D018809:Proliferating Cell Nuclear Antigen; D018036:Selenium Compounds; D051028:bcl-2-Associated X Protein; D019938:Cyclin D1; D016604:Aflatoxin B1; D053148:Caspase 3
Aflatoxin B1; Apoptosis; Broiler; Cell cycle; Renal cell; Selenium