Au@pNIPAM SERRS Tags for Multiplex Immunophenotyping Cellular Receptors and Imaging Tumor Cells.
Detection technologies employing optically encoded particles have gained much interest toward clinical diagnostics and drug discovery, but the portfolio of available systems is still limited. The fabrication and characterization of highly stable surface-enhanced resonance Raman scattering (SERRS)-encoded colloids for the identification and imaging of proteins expressed in cells are reported. These plasmonic nanostructures are made of gold octahedra coated with poly(N-isopropylacrylamide) microgels and can be readily encoded with Raman active dyes while retaining high colloidal stability in biofluids. A layer-by-layer polyelectrolyte coating is used to seal the outer surface of the encoded particles and to provide a reactive surface for covalent conjugation with antibodies. The targeted multiplexing capabilities of the SERRS tags are demonstrated by the simultaneous detection and imaging of three tumor-associated surface biomarkers: epidermal growth factor receptor (EGFR), epithelial cell adhesion molecule (EpCAM), and homing cell adhesion molecule (CD44) by SERRS spectroscopy. The plasmonic microgels are able to discriminate tumor A431 (EGFR+/EpCAM+/CD44+) and nontumor 3T3 2.2 (EGFR-/EpCAM-/CD44+) cells while cocultured in vitro.
Departmento de Química Física, Universidade de Vigo, 36310, Vigo, Spain.
Gustavo
G
Bodelón
Departmento de Química Física, Universidade de Vigo, 36310, Vigo, Spain.
Verónica
V
Montes-García
Departmento de Química Física, Universidade de Vigo, 36310, Vigo, Spain.
Cristina
C
Fernández-López
Departmento de Química Física, Universidade de Vigo, 36310, Vigo, Spain.
Isabel
I
Pastoriza-Santos
Departmento de Química Física, Universidade de Vigo, 36310, Vigo, Spain.
Jorge
J
Pérez-Juste
Departmento de Química Física, Universidade de Vigo, 36310, Vigo, Spain.|Bionanoplasmonics Laboratory, CIC biomaGUNE, Paseo de Miramón 182, 20009, Donostia-, San Sebastián, Spain.|Ikerbasque, Basque Foundation for Science, 48011, Bilbao, Spain.
Luis M
LM
Liz-Marzán
D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D002478:Cells, Cultured; D003952:Diagnostic Imaging / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D016130:Immunophenotyping / Q000379:methods*; D051379:Mice; D041681:NIH 3T3 Cells; D049329:Nanostructures / Q000737:chemistry; D009369:Neoplasms / Q000175:diagnosis* / Q000276:immunology / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000276:immunology / Q000378:metabolism*; D020349:Surface Plasmon Resonance / Q000379:methods
D000180:Acrylic Resins; D011956:Receptors, Cell Surface; C052970:poly-N-isopropylacrylamide; D006046:Gold
SERRS; bioimaging; gold nanoparticles; microgels; multiplex