Nanoparticulate STING agonists are potent lymph node-targeted vaccine adjuvants.
Cyclic dinucleotides (CDNs) are agonists of stimulator of IFN genes (STING) and have potential as vaccine adjuvants. However, cyclic di-GMP (cdGMP) injected s.c. shows minimal uptake into lymphatics/draining lymph nodes (dLNs) and instead is rapidly distributed to the bloodstream, leading to systemic inflammation. Here, we encapsulated cdGMP within PEGylated lipid nanoparticles (NP-cdGMP) to redirect this adjuvant to dLNs. Compared with unformulated CDNs, encapsulation blocked systemic dissemination and markedly enhanced dLN accumulation in murine models. Delivery of NP-cdGMP increased CD8+ T cell responses primed by peptide vaccines and enhanced therapeutic antitumor immunity. A combination of a poorly immunogenic liposomal HIV gp41 peptide antigen and NP-cdGMP robustly induced type I IFN in dLNs, induced a greater expansion of vaccine-specific CD4+ T cells, and greatly increased germinal center B cell differentiation in dLNs compared with a combination of liposomal HIV gp41 and soluble CDN. Further, NP-cdGMP promoted durable antibody titers that were substantially higher than those promoted by the well-studied TLR agonist monophosphoryl lipid A and comparable to a much larger dose of unformulated cdGMP, without the systemic toxicity of the latter. These results demonstrate that nanoparticulate delivery safely targets CDNs to the dLNs and enhances the efficacy of this adjuvant. Moreover, this approach can be broadly applied to other small-molecule immunomodulators of interest for vaccines and immunotherapy.
Melissa C
MC
Hanson
Monica P
MP
Crespo
Wuhbet
W
Abraham
Kelly D
KD
Moynihan
Gregory L
GL
Szeto
Stephanie H
SH
Chen
Mariane B
MB
Melo
Stefanie
S
Mueller
Darrell J
DJ
Irvine
D016915:AIDS Vaccines / Q000276:immunology / Q000494:pharmacology; D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D006152:Cyclic GMP / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D016503:Drug Delivery Systems; D015700:HIV Envelope Protein gp41 / Q000276:immunology / Q000494:pharmacology; D015497:HIV-1 / Q000276:immunology; D008050:Lipid A / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D008198:Lymph Nodes / Q000276:immunology*; D008565:Membrane Proteins / Q000819:agonists* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology; D000099197:STING Protein
D016915:AIDS Vaccines; D000276:Adjuvants, Immunologic; D006152:Cyclic GMP; D015700:HIV Envelope Protein gp41; D008050:Lipid A; D008565:Membrane Proteins; D011092:Polyethylene Glycols; C530855:Sting1 protein, mouse; D000099197:STING Protein; C514787:gp41 protein, Human immunodeficiency virus 1; C062025:bis(3',5')-cyclic diguanylic acid; C048436:monophosphoryl lipid A