PP2A (PR65) in Silver Carp: cDNA Cloning and Expression Analysis.
The full-length sequence of the protein phosphatase 2A subunit A (PP2A-A) cDNA from silver carp was cloned and sequenced. Additionally, the transcription of PP2A-A in the liver, kidney, and spleen of fish after microcystin (MC)-exposure were also determined in this study. The results reveal that PP2A-A cDNA is 2833 base pair (bp) long and contains an open reading frame of 1767 bp encoding a protein of 589 amino acids. Sequence analysis demonstrates that PP2A is highly conserved in fish. Furthermore, the results of quantitative real-time PCR indicate that PP2A-A is constitutively expressed in all examined silver carp tissues and primarily in the brain and liver. Additionally, we found that 50 or 200 μg/kg of crude MC exposure significantly downregulated PP2A-A transcription in fish liver, kidney, and spleen, suggesting that PP2A-A may be involved in MC toxicity in silver carp.
College of Life Science, Henan Normal University, Xinxiang, Henan, 453007, China.
Junguo
J
Ma
College of Life Science, Henan Normal University, Xinxiang, Henan, 453007, China.
Yiyi
Y
Feng
College of Life Science, Henan Normal University, Xinxiang, Henan, 453007, China.
Wenjie
W
Xie
College of Life Science, Henan Normal University, Xinxiang, Henan, 453007, China.
Xiaoyu
X
Li
Expression; Microcystins; PP2A-A: Silver Carp; cDNA Clone