Long-Term Fluoride Release from Dental Resins Affects STRO-1+ Cell Behavior.
Fluoride-releasing restorative dental materials can be beneficial to remineralize dentin and help prevent secondary caries. However, the effects of fluoride release from dental materials on the activity of dental pulp stem cells are not known. Here we investigate whether different fluoride release kinetics from dental resins supplemented with modified hydrotalcite (RK-F10) or fluoride-glass filler (RK-FG10) could influence the behavior of a human dental pulp stem cell subpopulation (STRO-1(+) cells) known for its ability to differentiate toward an odontoblast-like phenotype. The 2 resins, characterized by similar physicochemical properties and fluoride content, exhibited different long-term fluoride release kinetics. Our data demonstrate that long-term exposure of STRO-1(+) cells to a continuous release of a low amount of fluoride by RK-F10 increases their migratory response to transforming growth factor β1 (TGF-β1) and stromal cell-derived factor 1 (SDF-1), both important promoters of pulp stem cell recruitment. Moreover, the expression patterns of dentin sialoprotein (dspp), dentin matrix protein 1 (dmp1), osteocalcin (ocn), and matrix extracellular phosphoglycoprotein (mepe) indicate a complete odontoblast-like cell differentiation only when STRO-1(+) cells were cultured on RK-F10. On the contrary, RK-FG10, characterized by an initial fluoride release burst and reduced lifetime of the delivery, did not elicit any significant effect on both STRO-1(+) cell migration and differentiation. Taken together, our results highlight the importance of taking into account fluoride release kinetics in addition to fluoride concentration when designing new fluoride-restorative materials.
Institute of Bioscience and BioResources, CNR, Naples, Italy.
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Calarco
Institute of Bioscience and BioResources, CNR, Naples, Italy.
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Di Salle
Department of Industrial Engineering, University of Salerno, Fisciano, Salerno, Italy.
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Tammaro
Institute of Bioscience and BioResources, CNR, Naples, Italy.
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De Luca
Institute of Bioscience and BioResources, CNR, Naples, Italy.
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Mucerino
Institute of Bioscience and BioResources, CNR, Naples, Italy.
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Petillo
Conservative Odontostomatology and Maxillofacial Surgery, University of Naples, Naples, Italy.
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Riccitiello
Department of Industrial Engineering, University of Salerno, Fisciano, Salerno, Italy.
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Vittoria
Institute of Bioscience and BioResources, CNR, Naples, Italy gianfranco.peluso@ibbr.cnr.it g.peluso@cnr.it.
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Peluso
D000536:Aluminum Hydroxide / Q000737:chemistry; D002327:Cariostatic Agents / Q000493:pharmacokinetics*; D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000494:pharmacology; D003764:Dental Materials / Q000737:chemistry*; D003782:Dental Pulp / Q000166:cytology*; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005459:Fluorides / Q000493:pharmacokinetics*; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008276:Magnesium Hydroxide / Q000737:chemistry; D009805:Odontogenesis / Q000187:drug effects*; D015675:Osteocalcin / Q000378:metabolism; D010641:Phenotype; D010750:Phosphoproteins / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012464:Saliva, Artificial / Q000737:chemistry; D012795:Sialoglycoproteins / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects*; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology; D000099311:Dentin Sialophosphoprotein
D000536:Aluminum Hydroxide; D002327:Cariostatic Agents; D054377:Chemokine CXCL12; D003764:Dental Materials; D016326:Extracellular Matrix Proteins; D005459:Fluorides; D006023:Glycoproteins; D008276:Magnesium Hydroxide; D015675:Osteocalcin; D010750:Phosphoproteins; D012464:Saliva, Artificial; D012795:Sialoglycoproteins; D053773:Transforming Growth Factor beta1; D000099311:Dentin Sialophosphoprotein; C515255:CXCL12 protein, human; C513309:DMP1 protein, human; C486579:MEPE protein, human; C010467:hydrotalcite
cell migration; differentiation; fluoride-releasing materials; odontogenesis; pulp stem cells; restorative materials