SRC2-1 is required in PcINF1-induced pepper immunity by acting as an interacting partner of PcINF1.
Elicitins are elicitors that can trigger hypersensitive cell death in most Nicotiana spp., but their underlying molecular mechanism is not well understood. The gene Phytophthora capsici INF1 (PcINF1) coding for an elicitin from P. capsici was characterized in this study. Transient overexpression of PcINF1 triggered cell death in pepper (Capsicum annuum L.) and was accompanied by upregulation of the hypersensitive response marker, Hypersensitive Induced Reaction gene 1 (HIR1), and the pathogenesis-related genes SAR82, DEF1, BPR1, and PO2. A putative PcINF1-interacting protein, SRC2-1, was isolated from a pepper cDNA library by yeast two-hybrid screening and was observed to target the plasma membrane. The interaction between PcINF1 and SRC2-1 was confirmed by bimolecular fluorescence complementation and co-immunoprecipitation. Simultaneous transient overexpression of SRC2-1 and PcINF1 in pepper plants triggered intensive cell death, whereas silencing of SRC2-1 by virus-induced gene silencing blocked the cell death induction of PcINF1 and increased the susceptibility of pepper plants to P. capsici infection. Additionally, membrane targeting of the PcINF1-SRC2-1 complex was required for cell death induction. The C2 domain of SRC2-1 was crucial for SRC2-1 plasma membrane targeting and the PcINF1-SRC2-1 interaction. These results suggest that SRC2-1 interacts with PcINF1 and is required in PcINF1-induced pepper immunity.
National Education Minster Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China.
Zhi-qin
ZQ
Liu
National Education Minster Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China.
Ai-lian
AL
Qiu
National Education Minster Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China.
Lan-ping
LP
Shi
National Education Minster Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China.
Jin-sen
JS
Cai
National Education Minster Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China.
Xue-ying
XY
Huang
National Education Minster Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China.
Sheng
S
Yang
National Education Minster Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China.
Bo
B
Wang
National Education Minster Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China.
Lei
L
Shen
National Education Minster Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China.
Mu-kun
MK
Huang
National Education Minster Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China.
Shao-liang
SL
Mou
National Education Minster Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China.
Xiao-Ling
XL
Ma
National Education Minster Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China.
Yan-yan
YY
Liu
National Education Minster Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China.
Lin
L
Lin
National Education Minster Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China.
Jia-yu
JY
Wen
National Education Minster Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China.
Qian
Q
Tang
National Education Minster Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China.
Wei
W
Shi
National Education Minster Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China.
De-yi
DY
Guan
National Education Minster Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China.
Yan
Y
Lai
National Education Minster Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China shlhe201304@aliyun.com.
Shui-lin
SL
He
D002212:Capsicum / Q000276:immunology* / Q000382:microbiology*; D016923:Cell Death; D002462:Cell Membrane / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004198:Disease Susceptibility; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D047468:Immunoprecipitation; D010838:Phytophthora / Q000378:metabolism*; D010935:Plant Diseases / Q000276:immunology / Q000382:microbiology; D057865:Plant Immunity; D018515:Plant Leaves / Q000166:cytology; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D017422:Sequence Analysis, DNA
D010940:Plant Proteins; D011506:Proteins; C085101:elicitin, Phytophthora
Elicitin; Phytophthora capsici; hypersensitive response; interacting protein; membrane targeting; plant immunity.