Epsilon-caprolactone modified polyethylenimine for highly efficient antigen delivery and chemical exchange saturation transfer functional MR imaging.
Serious challenges remain in immunotherapy, such as to effectively deliver antigens and non-invasively monitor biological processes. Therefore, we aim to design a useful nanocarrier for enhancing antigen-specific immune response and performing functional molecular imaging guided immunotherapy. To achieve this goal, ε-caprolactone modified polyethylenimine (PEI-CL) was prepared based on the ring-opening reaction. This novel macromolecule served as an optimal vehicle that efficiently bound ovalbumin (OVA) antigen forming a controlled nanostructure through electronic self-assembly and resulted in a substantially increased cellular uptake. In vivo, PEI-CL/OVA nanovaccine stimulated inflammatory cytokine production and promoted proliferation of OVA-specific T cells. Moreover, due to the unique chemical and physical properties, PEI-CL was demonstrated as a chemical exchange saturation transfer contrast agent for magnetic resonance imaging. In conclusion, the PEI-CL has synergistic advantages in the effective delivery of antigen and non-invasive imaging for tracking immunotherapeutic cells.
Paul C. Lauterbur Research Center for Biomedical Imaging, Shenzhen Key Laboratory for MRI, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, PR China.
Yan
Y
Tan
Paul C. Lauterbur Research Center for Biomedical Imaging, Shenzhen Key Laboratory for MRI, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, PR China; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen 361005, PR China.
Lisi
L
Xie
Paul C. Lauterbur Research Center for Biomedical Imaging, Shenzhen Key Laboratory for MRI, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, PR China. Electronic address: zy.wang@siat.ac.cn.
Zhiyong
Z
Wang
Paul C. Lauterbur Research Center for Biomedical Imaging, Shenzhen Key Laboratory for MRI, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, PR China.
Na
N
Zhang
Paul C. Lauterbur Research Center for Biomedical Imaging, Shenzhen Key Laboratory for MRI, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, PR China.
Chao
C
Zou
Center for Gene and Cell Engineering, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, PR China.
Zhi-ying
ZY
Chen
Paul C. Lauterbur Research Center for Biomedical Imaging, Shenzhen Key Laboratory for MRI, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, PR China.
Xin
X
Liu
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, PR China.
Jian
J
Lu
State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen 361005, PR China.
Gang
G
Liu
Paul C. Lauterbur Research Center for Biomedical Imaging, Shenzhen Key Laboratory for MRI, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, PR China. Electronic address: hr.zheng@siat.ac.cn.
Hairong
H
Zheng
D000818:Animals; D000906:Antibodies / Q000737:chemistry; D000941:Antigens / Q000737:chemistry*; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology; D019496:Cancer Vaccines / Q000737:chemistry; D002208:Caproates / Q000737:chemistry*; D049109:Cell Proliferation; D002478:Cells, Cultured; D003287:Contrast Media / Q000737:chemistry; D016207:Cytokines / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry; D007167:Immunotherapy / Q000295:instrumentation* / Q000379:methods; D007783:Lactones / Q000737:chemistry*; D046911:Macromolecular Substances; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D053758:Nanoparticles / Q000737:chemistry; D010047:Ovalbumin / Q000737:chemistry; D011094:Polyethyleneimine / Q000737:chemistry*; D000067996:RAW 264.7 Cells
D000906:Antibodies; D000941:Antigens; D019496:Cancer Vaccines; D002208:Caproates; D003287:Contrast Media; D016207:Cytokines; D005456:Fluorescent Dyes; D007783:Lactones; D046911:Macromolecular Substances; C121056:caprolactone; D011094:Polyethyleneimine; D010047:Ovalbumin
Adjuvant; Chemical exchange saturation transfer effect; Immunization; Magnetic resonance imaging; Nanovaccine