Micropatterned bioimplant with guided neuronal cells to promote tissue reconstruction and improve functional recovery after primary motor cortex insult.
With the ever increasing incidence of brain injury, developing new tissue engineering strategies to promote neural tissue regeneration is an enormous challenge. The goal of this study was to design and evaluate an implantable scaffold capable of directing neurite and axonal growth for neuronal brain tissue regeneration. We have previously shown in cell culture conditions that engineered micropatterned PDMS surface with straight microchannels allow directed neurite growth without perturbing cell differentiation and neurite outgrowth. In this study, the micropatterned PDMS device pre-seeded with hNT2 neuronal cells were implanted in rat model of primary motor cortex lesion which induced a strong motor deficit. Functional recovery was assessed by the forelimb grip strength test during 3 months post implantation. Results show a more rapid and efficient motor recovery with the hNT2 neuroimplants associated with an increase of neuronal tissue reconstruction and cell survival. This improvement is also hastened when compared to a direct cell graft of ten times more cells. Histological analyses showed that the implant remained structurally intact and we did not see any evidence of inflammatory reaction. In conclusion, PDMS bioimplants with guided neuronal cells seem to be a promising approach for supporting neural tissue reconstruction after central brain injury.
INSERM, UMR 825 Imagerie Cérébrale et Handicaps Neurologiques, F-31024 Toulouse, France; Université Toulouse 3 Paul Sabatier, UMR 825 Imagerie Cérébrale et Handicaps, Neurologiques, F-31024 Toulouse, France. Electronic address: laurence.vaysse@inserm.fr.
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Vaysse
CNRS-LAAS, 7 avenue du colonel Roche, F-31400 Toulouse, France.
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Beduer
INSERM, UMR 825 Imagerie Cérébrale et Handicaps Neurologiques, F-31024 Toulouse, France; Université Toulouse 3 Paul Sabatier, UMR 825 Imagerie Cérébrale et Handicaps, Neurologiques, F-31024 Toulouse, France; Pôle Neurosciences, Centre Hospitalier Universitaire de Toulouse, Toulouse, France.
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Sol
CNRS-LAAS, 7 avenue du colonel Roche, F-31400 Toulouse, France; Univ de Toulouse, INSA, LAAS, F-31400 Toulouse, France.
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Vieu
INSERM, UMR 825 Imagerie Cérébrale et Handicaps Neurologiques, F-31024 Toulouse, France; Université Toulouse 3 Paul Sabatier, UMR 825 Imagerie Cérébrale et Handicaps, Neurologiques, F-31024 Toulouse, France.
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Loubinoux
D000818:Animals; D001369:Axons / Q000378:metabolism; D001522:Behavior, Animal; D001705:Bioprosthesis; D001930:Brain Injuries / Q000628:therapy; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004867:Equipment Design; D005904:Glial Fibrillary Acidic Protein / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009044:Motor Cortex / Q000293:injuries* / Q000473:pathology*; D016501:Neurites / Q000378:metabolism; D055495:Neurogenesis; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D019736:Prostheses and Implants; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds
D004129:Dimethylpolysiloxanes; D005904:Glial Fibrillary Acidic Protein; C013830:baysilon
Bioprosthesis; Brain injury; Graft micropatterned surface; Neural stem cell; Tissue bioengineering