Contribution of Musa paradisiaca in the inhibition of α-amylase, α-glucosidase and Angiotensin-I converting enzyme in streptozotocin induced rats.
OBJECTIVE
Unripe plantain based-diets are part of folklore remedy for the management of diabetes in tropical Africa; however, with the dearth of information on the rationale behind this practice; this study therefore, sought to investigate the antihyperglycemic effect of traditional unripe plantain products (Amala and Booli) in high fat fed/low dose streptozotocin-induced diabetic rats and to provide a possible rationale for their antidiabetic properties.


METHODS
Diabetes was induced experimentally by high fat fed/low dose streptozotocin-diabetic rats (25mg/kg body wt.) and the diabetic rats were fed diets supplemented with 20-40% Amala and Booli for 14 days. The effect of the diets on the blood glucose level, pancreatic α-amylase, intestinal α-glucosidase and Angiotensin-I converting enzyme (ACE) activities and plasma antioxidant status as well as amylose/amylopectin content of the unripe plantain products were determined.


RESULTS
A marked increase in the blood glucose, α-amylase, α-glucosidase and ACE activities with a corresponding decrease in plasma antioxidant status was recorded in diabetic rats. However, these indices were significantly (P < 0.05) reversed after unripe plantain product supplemented diet treatments for 14 days. Also, the amylose/amylopectin ratio of the products is 1:3.


CONCLUSIONS
This study revealed that unripe plantain products exert antihyperglycemic effects which could be attributed to the inhibition of α-amylase and α-glucosidase activities by their constituent phytochemicals as well as their amylose/amylopectin contents in the diabetic rats, hence, providing the possible rationale behind their antidiabetic properties.
Department of Biochemistry, Adekunle Ajasin University, P.M.B. 01, Akungba Akoko, Ondo State, Nigeria; Functional Foods and Nutraceuticals Unit, Department of Biochemistry, Federal University of Technology, P.M.B. 704, Akure 340001, Nigeria. Electronic address: adamsonabike@yahoo.com.
Sidiqat A
SA
Shodehinde
Functional Foods and Nutraceuticals Unit, Department of Biochemistry, Federal University of Technology, P.M.B. 704, Akure 340001, Nigeria.
Adedayo O
AO
Ademiluyi
Functional Foods and Nutraceuticals Unit, Department of Biochemistry, Federal University of Technology, P.M.B. 704, Akure 340001, Nigeria.
Ganiyu
G
Oboh
Functional Foods and Nutraceuticals Unit, Department of Biochemistry, Federal University of Technology, P.M.B. 704, Akure 340001, Nigeria.
Afolabi A
AA
Akindahunsi
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000178:diet therapy* / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000139:chemically induced / Q000178:diet therapy / Q000378:metabolism; D019587:Dietary Supplements / Q000032:analysis; D005638:Fruit / Q000378:metabolism; D008297:Male; D028521:Musa / Q000378:metabolism; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin; D000516:alpha-Amylases / Q000378:metabolism*; D000520:alpha-Glucosidases / Q000378:metabolism*
D000975:Antioxidants; D001786:Blood Glucose; D013311:Streptozocin; D000516:alpha-Amylases; D000520:alpha-Glucosidases; D007703:Peptidyl-Dipeptidase A
Antioxidants; Streptozotocin; Type 2 diabetes; Unripe plantain; α-Amylase; α-Glucosidase