[Research advances of the influence factors of high level expression of recombinant protein in Pichia pastoris].
Pichia pastoris is one of the most important systems used in the field of molecular biology for the expression of recombinant proteins. The system has advantages of high expression, high stability, high secretion, easy high-density fermentation and low cost. Many factors affect the expression of recombinant protein, such as gene copy number, codon usage preference, type of promoter, molecular chaperones, glycosylation, signal peptide and fermentation process. In this review, research advances of the above aspects are summarized, which lay a foundation for improving the expression of recombinant proteins in P. pastoris.
Qing-Hua
QH
Wang
Li-Li
LL
Gao
Hui-Chao
HC
Liang
Ting
T
Gong
Jin-Ling
JL
Yang
Ping
P
Zhu
D005285:Fermentation; D018628:Gene Dosage; D006031:Glycosylation; D018832:Molecular Chaperones; D010843:Pichia / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D021382:Protein Sorting Signals; D011994:Recombinant Proteins / Q000096:biosynthesis*
D018832:Molecular Chaperones; D021382:Protein Sorting Signals; D011994:Recombinant Proteins