Multilayer hydrogel coatings to combine hemocompatibility and antimicrobial activity.
While silver-loaded catheters are widely used to prevent early-onset catheter-related infections [1], long term antimicrobial protection of indwelling catheters remains to be achieved [2] and antiseptic functionalization of coatings often impairs their hemocompatibility characteristics. Therefore, this work aimed to capitalize on the antimicrobial properties of silver nanoparticles, incorporated in anticoagulant poly(ethylene glycol) (PEG)-heparin hydrogel coatings [3] on thermoplastic polyurethane materials. For prolonged antimicrobial activity, the silver-containing starPEG-heparin hydrogel layers were shielded with silver-free hydrogel layers of otherwise similar composition. The resulting multi-layered gel coatings showed long term antiseptic efficacy against Escherichia coli and Staphylococcus epidermidis strains in vitro, and similarly performed well when incubated with freshly drawn human whole blood with respect to hemolysis, platelet activation and plasmatic coagulation. The introduced hydrogel multilayer system thus offers a promising combination of hemocompatibility and long-term antiseptic capacity to meet an important clinical need.
Max Bergmann Center of Biomaterials Dresden, Leibniz Institute of Polymer Research Dresden, Hohe Str. 6, 01069 Dresden, Germany.
Marion
M
Fischer
Max Bergmann Center of Biomaterials Dresden, Leibniz Institute of Polymer Research Dresden, Hohe Str. 6, 01069 Dresden, Germany.
Maryam
M
Vahdatzadeh
BASF SE, Advanced Materials and Systems Research, D-67056 Ludwigshafen, Germany.
Rupert
R
Konradi
Max Bergmann Center of Biomaterials Dresden, Leibniz Institute of Polymer Research Dresden, Hohe Str. 6, 01069 Dresden, Germany.
Jens
J
Friedrichs
Max Bergmann Center of Biomaterials Dresden, Leibniz Institute of Polymer Research Dresden, Hohe Str. 6, 01069 Dresden, Germany.
Manfred F
MF
Maitz
Max Bergmann Center of Biomaterials Dresden, Leibniz Institute of Polymer Research Dresden, Hohe Str. 6, 01069 Dresden, Germany.
Uwe
U
Freudenberg
Max Bergmann Center of Biomaterials Dresden, Leibniz Institute of Polymer Research Dresden, Hohe Str. 6, 01069 Dresden, Germany. Electronic address: werner@ipfdd.de.
Carsten
C
Werner
D000890:Anti-Infective Agents / Q000737:chemistry*; D000891:Anti-Infective Agents, Local / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry*; D001777:Blood Coagulation; D002408:Catheters, Indwelling / Q000382:microbiology; D016503:Drug Delivery Systems; D004926:Escherichia coli / Q000187:drug effects; D006461:Hemolysis; D006493:Heparin / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D015539:Platelet Activation; D011092:Polyethylene Glycols / Q000737:chemistry*; D011140:Polyurethanes / Q000737:chemistry; D012212:Rheology; D012834:Silver / Q000737:chemistry*; D012835:Silver Nitrate / Q000737:chemistry; D013212:Staphylococcus epidermidis / Q000187:drug effects
D000890:Anti-Infective Agents; D000891:Anti-Infective Agents, Local; D001672:Biocompatible Materials; D020100:Hydrogels; D011140:Polyurethanes; D012834:Silver; D011092:Polyethylene Glycols; D006493:Heparin; D012835:Silver Nitrate
Antibacterial; Drug release; Hemocompatibility; Hydrogel; Silver