Proteomic Analysis of the Endosperm Ontogeny of Jatropha curcas L. Seeds.
Seeds of Jatropha curcas L. represent a potential source of raw material for the production of biodiesel. However, this use is hampered by the lack of basic information on the biosynthetic pathways associated with synthesis of toxic diterpenes, fatty acids, and triacylglycerols, as well as the pattern of deposition of storage proteins during seed development. In this study, we performed an in-depth proteome analysis of the endosperm isolated from five developmental stages which resulted in the identification of 1517, 1256, 1033, 752, and 307 proteins, respectively, summing up 1760 different proteins. Proteins with similar label free quantitation expression pattern were grouped into five clusters. The biological significance of these identifications is discussed with special focus on the analysis of seed storage proteins, proteins involved in the metabolism of fatty acids, carbohydrates, toxic components and proteolytic processing. Although several enzymes belonging to the biosynthesis of diterpenoid precursors were identified, we were unable to find any terpene synthase/cyclase, indicating that the synthesis of phorbol esters, the main toxic diterpenes, does not occur in seeds. The strategy used enabled us to provide a first in depth proteome analysis of the developing endosperm of this biodiesel plant, providing an important glimpse into the enzymatic machinery devoted to the production of C and N sources to sustain seed development.
†Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza 60455-900, Ceara, Brazil.
Mohibullah
M
Shah
†Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza 60455-900, Ceara, Brazil.
Emanoella L
EL
Soares
‡Laboratory for Proteomics and Protein Engineering, Carlos Chagas Institute, Fiocruz, Paraná 81350-010, Brazil.
Paulo C
PC
Carvalho
∥Department of Biology, Federal University of Ceara, Fortaleza 60455-900, Ceara, Brazil.
Arlete A
AA
Soares
§Proteomic Unit, Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro 21941-909, Rio de Janeiro, Brazil.
Gilberto B
GB
Domont
§Proteomic Unit, Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro 21941-909, Rio de Janeiro, Brazil.
Fábio C S
FC
Nogueira
†Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza 60455-900, Ceara, Brazil.
Francisco A P
FA
Campos
D031286:Jatropha / Q000196:embryology*; D040901:Proteomics; D012639:Seeds / Q000254:growth & development* / Q000378:metabolism
Jatropha curcas; casbene synthase; oilseeds; peptidases; seed development; storage proteins; terpenoids