Regulation of flowering time by the histone deacetylase HDA5 in Arabidopsis.
The acetylation level of histones on lysine residues regulated by histone acetyltransferases and histone deacetylases plays an important but under-studied role in the control of gene expression in plants. With the aim of characterizing the Arabidopsis RPD3/HDA1 family histone deacetylase HDA5, we present evidence showing that HDA5 displays deacetylase activity. Mutants defective in the expression of HDA5 displayed a late-flowering phenotype. Expression of the flowering repressor genes FLC and MAF1 was up-regulated in hda5 mutants. Furthermore, the gene activation markers, histone H3 acetylation and H3K4 trimethylation on FLC and MAF1 chromatin were increased in hda5-1 mutants. Chromatin immunoprecipitation analysis showed that HDA5 binds to the chromatin of FLC and MAF1. Bimolecular fluorescence complementation assays and co-immunoprecipitation assays showed that HDA5 interacts with FVE, FLD and HDA6, indicating that these proteins are present in a protein complex involved in the regulation of flowering time. Comparing gene expression profiles of hda5 and hda6 mutants by RNA-seq revealed that HDA5 and HDA6 co-regulate gene expression in multiple development processes and pathways.
Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, No. 723, Xingke Road, 510650, Guangzhou, China.|Institute of Plant Biology, College of Life Science, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, 10617, Taipei, Taiwan.
Ming
M
Luo
Institute of Plant Biology, College of Life Science, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, 10617, Taipei, Taiwan.
Ready
R
Tai
Institute of Plant Biology, College of Life Science, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, 10617, Taipei, Taiwan.
Chun-Wei
CW
Yu
Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, No. 723, Xingke Road, 510650, Guangzhou, China.
Songguang
S
Yang
Institute of Plant Biology, College of Life Science, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, 10617, Taipei, Taiwan.
Chia-Yang
CY
Chen
Institute of Plant and Microbial Biology, Academia Sinica, No. 128, Sec. 2, Academia Road, 11529, Taipei, Taiwan.
Wen-Dar
WD
Lin
Institute of Plant and Microbial Biology, Academia Sinica, No. 128, Sec. 2, Academia Road, 11529, Taipei, Taiwan.
Wolfgang
W
Schmidt
Institute of Plant Biology, College of Life Science, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, 10617, Taipei, Taiwan.
Keqiang
K
Wu
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D047369:Chromatin Immunoprecipitation; D035264:Flowers / Q000235:genetics / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D006655:Histone Deacetylases / Q000235:genetics / Q000378:metabolism*; D026161:MADS Domain Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D014157:Transcription Factors
D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D002843:Chromatin; C418902:At5g10140 protein, Arabidopsis; C522476:FLM protein, Arabidopsis; D026161:MADS Domain Proteins; C116635:MSI4 protein, Arabidopsis; D014157:Transcription Factors; C432452:AT5G63110 protein, Arabidopsis; C479923:flowering locus D protein, Arabidopsis; C000610747:HDA5 protein, Arabidopsis; D006655:Histone Deacetylases
Arabidopsis; HDA5; HDA6; flowering; histone deacetylase