Preventative effects of prostaglandin E1 in combination with iodized olive oil on liver fibrosis after transcatheter arterial chemoembolization in a rabbit model of CCl4-induced liver fibrosis.
To explore the preventative effects of prostaglandin E1 (PGE1) on a rabbit model of CCl4-induced liver fibrosis after transcatheter arterial chemoembolization (TACE), we generated a rabbit model of CCl4-induced liver fibrosis by treatment with 40% CCl4 in iodized olive oil for 16 weeks. Body mass and serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total protein (TP), albumin (ALB), albumin:globulin ratio (A:G), total bilirubin (TBIL), and direct bilirubin (DBIL) were measured. After TACE, the levels of hyaluronic acid (HA), procollagen III (PC III), laminin (LN), and collagen IV (IV-C) were measured, and the severity of liver fibrosis as well as the morphology of liver tissues were determined. Body mass in the model group was significantly decreased from 10 to 16 weeks, and the serum levels of ALT, AST, TP, TBIL, and DBIL levels were significantly increased while the model was being generated; the levels of ALB and A:G were significantly decreased. After TACE, serum levels of HA, PC III, and LN in the group injected with 1.0 mL iodized olive oil (Group B) were higher than in the group that were injected with 1.0 mL iodized olive oil + 0.2 mL PGE1 (Group C), whereas the serum levels of IV-C were lower. The severity of liver fibrosis was ameliorated in Group C. The combination of PGE1 and iodized olive oil prevented the development of liver fibrosis following TACE.
Department of Interventional Radiology, the Second Affiliated Hospital of Harbin Medical University, No. 246 Xuefu Road, Harbin 150086, Heilongjiang Province, China.
Shuqiang
S
Jin
Haili
H
Cao
Kaibing
K
Wang
Ying
Y
Li
Bin
B
Bai
D000410:Alanine Transaminase / Q000378:metabolism; D000527:Alprostadil / Q000494:pharmacology*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D001663:Bilirubin / Q000378:metabolism; D002251:Carbon Tetrachloride / Q000494:pharmacology*; D003094:Collagen / Q000378:metabolism; D006820:Hyaluronic Acid / Q000378:metabolism; D007459:Iodized Oil / Q000494:pharmacology*; D007797:Laminin / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008106:Liver Cirrhosis, Experimental / Q000139:chemically induced* / Q000378:metabolism / Q000517:prevention & control*; D008111:Liver Function Tests / Q000379:methods; D000069463:Olive Oil / Q000494:pharmacology*; D011817:Rabbits
D007797:Laminin; D000069463:Olive Oil; D007459:Iodized Oil; D006820:Hyaluronic Acid; D003094:Collagen; D002251:Carbon Tetrachloride; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000527:Alprostadil; D001663:Bilirubin
acide hyaluronique; collagen IV; collagène IV; femoral artery puncture; huile iodée; hyaluronic acid; iodized olive oil; laminin; laminine; perforation de l’artère fémorale; procollagen III; procollagène III