Polymorphisms and expression analysis of SOX-6 in relation to porcine growth, carcass, and meat quality traits.
The aim of the study was to investigate single nucleotide polymorphisms (SNPs) and expression of SOX-6 to support its candidacy for growth, carcass, and meat quality traits in pigs. The first SNP, rs81358375, was associated with pH 45 min post mortem in loin (pH1L), the thickness of backfat and side fat, and carcass length in Pietrain (Pi) population, and related with backfat thickness and daily gain in Duroc × Pietrain F2 (DuPi) population. The other SNP, rs321666676, was associated with meat colour in Pi population. In DuPi population, the protein, not mRNA, level of SOX-6 in high pH1L pigs was significantly less abundant compared with low pH1L pigs, where microRNAs targeting SOX-6 were also differently regulated. This paper shows that SOX-6 could be a potential candidate gene for porcine growth, carcass, and meat quality traits based on genetic association and gene expression.
Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, 53115 Bonn, Germany. Electronic address: rzha@itw.uni-bonn.de.
Rui
R
Zhang
Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, 53115 Bonn, Germany. Electronic address: cgro@itw.uni-bonn.de.
Christine
C
Große-Brinkhaus
Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, 53115 Bonn, Germany. Electronic address: hhei@itw.uni-bonn.de.
Hanna
H
Heidt
Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, 53115 Bonn, Germany; Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh. Electronic address: judd@itw.uni-bonn.de.
Muhammad Jasim
MJ
Uddin
Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, 53115 Bonn, Germany; Faculty of Agriculture, Department of Animal Science, Erciyes University, 38039 Kayseri, Turkey. Electronic address: ucin@itw.uni-bonn.de.
Mehmet Ulas
MU
Cinar
Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, 53115 Bonn, Germany. Electronic address: dtes@itw.uni-bonn.de.
Dawit
D
Tesfaye
Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, 53115 Bonn, Germany. Electronic address: etho@itw.uni-bonn.de.
Ernst
E
Tholen
Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, 53115 Bonn, Germany. Electronic address: cloo@itw.uni-bonn.de.
Christian
C
Looft
Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, 53115 Bonn, Germany. Electronic address: ksch@itw.uni-bonn.de.
Karl
K
Schellander
Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, 53115 Bonn, Germany. Electronic address: cneu@itw.uni-bonn.de.
Christiane
C
Neuhoff
D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D001823:Body Composition; D001947:Breeding; D003116:Color; D015870:Gene Expression; D056726:Genetic Association Studies; D005838:Genotype; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D012333:RNA, Messenger / Q000378:metabolism; D000069466:Red Meat / Q000032:analysis* / Q000592:standards; D055755:SOXD Transcription Factors / Q000235:genetics* / Q000378:metabolism; D013552:Swine / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
D035683:MicroRNAs; D012333:RNA, Messenger; D055755:SOXD Transcription Factors
Carcass; Growth; Meat quality; MicroRNA; SOX-6; Single nucleotide polymorphism