Development of a colloidal gold immunochromatographic strip for the rapid detection of soft-shelled turtle systemic septicemia spherical virus.
A colloidal gold immunochromatographic strip (ICS) test based on a competitive format was developed for the rapid detection of soft-shelled turtle systemic septicemia spherical virus (STSSSV) in turtle and fecal samples. Specific egg yolk antibodies (IgY) against STSSSV were labeled with colloidal gold and used as probes in the one-step test strip. Antigen (STSSSV) and goat anti-chicken IgY were drawn on the nitrocellulose membrane as the test line and control line, respectively. When STSSSV standard samples (0-100μg/mL) were detected by the strips, the visual limit of detection (LOD) was found to be 50.0μg/mL. The ICS test showed high stability; the strips were stable for at least 3 months at 4°C without significant loss of activity. There was no obvious cross-reactivity with other aquatic pathogens. The assay can be performed within 5-10min. Analysis of STSSSV in turtle samples revealed that data obtained from the ICS test were in a good agreement with those obtained by ELISA. The positive results of fecal samples suggested that this method could be used to detect STSSSV while protecting the animals' welfare. The ICS assay does not need specialized equipment or a technician and can be used as a reliable, rapid, cost-effective and convenient qualitative tool for on-site diagnosis.
School of Marine Sciences, Ningbo University, Fenghua Road, Ningbo, Zhejiang 315211, PR China. Electronic address: zhangliping821@126.com.
Liping
L
Zhang
School of Marine Sciences, Ningbo University, Fenghua Road, Ningbo, Zhejiang 315211, PR China. Electronic address: lidengfeng@nbu.edu.cn.
Dengfeng
D
Li
School of Marine Sciences, Ningbo University, Fenghua Road, Ningbo, Zhejiang 315211, PR China.
Lianguo
L
Liu
School of Marine Sciences, Ningbo University, Fenghua Road, Ningbo, Zhejiang 315211, PR China.
Jing
J
Fang
School of Marine Sciences, Ningbo University, Fenghua Road, Ningbo, Zhejiang 315211, PR China.
Ran
R
Xu
College of Biological Science and Technology, Hunan Agriculture University, Nongda Road, Changsha, Hunan 410128, PR China.
Ge
G
Zhang
D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods*; D005243:Feces / Q000821:virology; D017979:Gold Colloid / Q000378:metabolism*; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods*; D013997:Time Factors; D014426:Turtles / Q000821:virology*
D017979:Gold Colloid
Egg yolk antibody (IgY); Gold nanoparticle; Immunochromatographic strip; Soft-shelled turtle systemic septicemia spherical virus (STSSSV)