Light-Activated Proteolysis for the Spatiotemporal Control of Proteins.
The regulation of proteolysis is an efficient way to control protein function in cells. Here, we present a general strategy enabling to increase the spatiotemporal resolution of conditional proteolysis by using light activation as trigger. Our approach relies on the auxin-inducible degradation system obtained by transposing components of the plant auxin-dependent degradation pathway in mammalian cells. We developed a photoactivatable auxin that acts as a photoactivatable inducer of degradation. Upon local and short light illumination, auxin is released in cells and triggers the degradation of a protein of interest with spatiotemporal control.
†Department of Chemistry, École Normale Supérieure-PSL Research University, 24 rue Lhomond, F-75005 Paris, France.|‡Sorbonne Universités, UPMC Univ Paris 06, UMR 8640 PASTEUR, F-75005 Paris, France.|§CNRS, UMR 8640 PASTEUR, F-75005 Paris, France.
Quentin
Q
Delacour
†Department of Chemistry, École Normale Supérieure-PSL Research University, 24 rue Lhomond, F-75005 Paris, France.|‡Sorbonne Universités, UPMC Univ Paris 06, UMR 8640 PASTEUR, F-75005 Paris, France.|§CNRS, UMR 8640 PASTEUR, F-75005 Paris, France.
Chenge
C
Li
†Department of Chemistry, École Normale Supérieure-PSL Research University, 24 rue Lhomond, F-75005 Paris, France.|‡Sorbonne Universités, UPMC Univ Paris 06, UMR 8640 PASTEUR, F-75005 Paris, France.|§CNRS, UMR 8640 PASTEUR, F-75005 Paris, France.
Marie-Aude
MA
Plamont
†Department of Chemistry, École Normale Supérieure-PSL Research University, 24 rue Lhomond, F-75005 Paris, France.|‡Sorbonne Universités, UPMC Univ Paris 06, UMR 8640 PASTEUR, F-75005 Paris, France.|§CNRS, UMR 8640 PASTEUR, F-75005 Paris, France.
Emmanuelle
E
Billon-Denis
†Department of Chemistry, École Normale Supérieure-PSL Research University, 24 rue Lhomond, F-75005 Paris, France.|‡Sorbonne Universités, UPMC Univ Paris 06, UMR 8640 PASTEUR, F-75005 Paris, France.|§CNRS, UMR 8640 PASTEUR, F-75005 Paris, France.
Isabelle
I
Aujard
†Department of Chemistry, École Normale Supérieure-PSL Research University, 24 rue Lhomond, F-75005 Paris, France.|‡Sorbonne Universités, UPMC Univ Paris 06, UMR 8640 PASTEUR, F-75005 Paris, France.|§CNRS, UMR 8640 PASTEUR, F-75005 Paris, France.
Thomas
T
Le Saux
†Department of Chemistry, École Normale Supérieure-PSL Research University, 24 rue Lhomond, F-75005 Paris, France.|‡Sorbonne Universités, UPMC Univ Paris 06, UMR 8640 PASTEUR, F-75005 Paris, France.|§CNRS, UMR 8640 PASTEUR, F-75005 Paris, France.
Ludovic
L
Jullien
†Department of Chemistry, École Normale Supérieure-PSL Research University, 24 rue Lhomond, F-75005 Paris, France.|‡Sorbonne Universités, UPMC Univ Paris 06, UMR 8640 PASTEUR, F-75005 Paris, France.|§CNRS, UMR 8640 PASTEUR, F-75005 Paris, France.
Arnaud
A
Gautier
D000818:Animals; D057809:HEK293 Cells; D006801:Humans; D007210:Indoleacetic Acids / Q000378:metabolism*; D008027:Light; D010782:Photolysis; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D059748:Proteolysis; D025801:Ubiquitin / Q000378:metabolism
D007210:Indoleacetic Acids; D025801:Ubiquitin; D046988:Proteasome Endopeptidase Complex