Evaluation of recombinant Neospora caninum antigens purified from silkworm larvae for the protection of N. caninum infection in mice.
Three antigens (NcSAG1, NcSRS2 and NcMIC3) from Neospora caninum were expressed using the BmNPV bacmid system in silkworm larvae and purified from the hemolymph. From 20 silkworm larvae, 1.5, 1.2 and 1.4 mg of purified recombinant NcSAG1, NcSRS2 and NcMIC3 were obtained, respectively. When each purified recombinant antigen was immunized with Freund's incomplete adjuvant (FIA) to mice, recombinant NcSAG1 induced a Th2 immune response in immunized mice and produced a SAG1-specific antibody. In the experiment where NcSAG1-immunized mice were challenged with N. caninum, the cerebral N. caninum burden was significantly reduced compared with that of either the FIA- or PBS-immunized mice. Recombinant NcSRS2 or NcMIC3 induced both Th1 and Th2 immune responses, but NcMIC3-immunization did not induce significant production of NcMIC3-specific antibodies. These results suggest that the silkworm can produce recombinant antigens of N. caninum, which can be used as a recombinant vaccine against N. caninum.
Laboratory of Biotechnology, Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.
Mai
M
Yoshimoto
Laboratory of Biotechnology, Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.
Takahiro
T
Otsuki
Laboratory of Biotechnology, Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.
Kohei
K
Itagaki
Laboratory of Biotechnology, Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan; Laboratory of Biotechnology, Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.
Tatsuya
T
Kato
Laboratory of Animal Reproduction & Physiology, Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Suruga-ku, Shizuoka 422-8529, Japan.
Tetsuya
T
Kohsaka
Laboratory of Veterinary Parasitology, Nippon Veterinary and Life University, Musashino, Tokyo 180-8602, Japan.
Yumino
Y
Matsumoto
Laboratory of Veterinary Parasitology, Nippon Veterinary and Life University, Musashino, Tokyo 180-8602, Japan.
Kazunori
K
Ike
Laboratory of Biotechnology, Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan; Laboratory of Biotechnology, Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan. Electronic address: park.enoch@shizuoka.ac.jp.
Enoch Y
EY
Park
D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification*; D012831:Bombyx; D003048:Coccidiosis / Q000276:immunology* / Q000517:prevention & control*; D005260:Female; D006458:Hemolymph / Q000737:chemistry; D007114:Immunization; D007814:Larva; D051379:Mice; D008807:Mice, Inbred BALB C; D018688:Neospora / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D016052:Protozoan Vaccines / Q000737:chemistry / Q000276:immunology; D011994:Recombinant Proteins / Q000302:isolation & purification; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D022223:Vaccines, Subunit / Q000737:chemistry / Q000276:immunology
D000953:Antigens, Protozoan; D016052:Protozoan Vaccines; D011994:Recombinant Proteins; D022223:Vaccines, Subunit
Antigen; BmNPV bacmid; Neospora caninum; Silkworm; Subunit vaccine