An electrical conductivity based method of determining the particle deposition rate in air-liquid interface devices.
A new in-situ method of determining the particle deposition rate onto cell cultures inside air-liquid interface devices is described. It is based on depositing a surrogate aerosol of salt particles onto the water filled wells of a culture plate while measuring the resulting change in electrical conductivity of the solution in situ, in order to derive the accumulated particle mass. For evaluation purposes, the wells of a six-well cell culture plate were equipped with custom designed electrodes and calibrated with a series of commercially available standard solutions. After the necessary corrections prescribed by theory, the calibration resulted in an accuracy and comparability between cells of ±3% in terms of measured conductivity. The method was then applied to a specific ALI device consisting essentially of the calibrated six-well culture plate inside an electrostatic cross-flow precipitator, and tested with submicron NaCl aerosol of defined size distribution produced by nebulization of a salt solution. 2h of particle accumulation were sufficient to accumulate between 30 and 10 μg of salt per well, depending on the location in the precipitator. Resulting deposition rates varied narrowly between the wells by about 2 ng min(-1) cm(-2). Factors affecting the overall accuracy and reproducibility are discussed.
Institute for Mechanical Process Engineering and Mechanics, Karlsruhe Institute of Technology (KIT), Straße am Forum 8, D-76131 Karlsruhe, Germany. Electronic address: harald.wiegand@kit.edu.
Harald
H
Wiegand
Institute for Mechanical Process Engineering and Mechanics, Karlsruhe Institute of Technology (KIT), Straße am Forum 8, D-76131 Karlsruhe, Germany.
Jörg
J
Meyer
Institute for Mechanical Process Engineering and Mechanics, Karlsruhe Institute of Technology (KIT), Straße am Forum 8, D-76131 Karlsruhe, Germany.
Gerhard
G
Kasper
D000336:Aerosols; D000388:Air; D018929:Cell Culture Techniques; D004553:Electric Conductivity; D004566:Electrodes; D012965:Sodium Chloride / Q000737:chemistry
D000336:Aerosols; D012965:Sodium Chloride
Air–liquid interface; Deposition efficiency; Deposition rate; Electrical conductivity; Nanoparticles