Evaluation of Surface Microtopography Engineered by Direct Laser Interference for Bacterial Anti-Biofouling.
Modification of the biomaterial surface topography is a promising strategy to prevent bacterial adhesion and biofilm formation. In this study, we use direct laser interference patterning (DLIP) to modify polystyrene surface topography at sub-micrometer scale. The results revealed that three-dimensional micrometer structures have a profound impact on bacterial adhesion. Thus, line- and pillar-like patterns enhanced S. aureus adhesion, whereas complex lamella microtopography reduced S. aureus adhesion in static and continuous flow culture conditions. Interestingly, lamella-like textured surfaces retained the capacity to inhibit S. aureus adhesion both when the surface is coated with human serum proteins and when the material is implanted subcutaneously in a foreign-body associated infection model.
Laboratory of Microbial Biofilms, Instituto de Agrobiotecnología, Universidad Pública de Navarra-CSIC-Gobierno de Navarra, Campus de Arrosadía, Pamplona, Spain. jaione.valle@unavarra.es.
Jaione
J
Valle
Laboratory of Microbial Biofilms, Instituto de Agrobiotecnología, Universidad Pública de Navarra-CSIC-Gobierno de Navarra, Campus de Arrosadía, Pamplona, Spain.
Saioa
S
Burgui
Fraunhofer Institute for Material and Beam Technology (IWS) Dresden, Winterbergstraße 28, 01277 Dresden, Germany.|Institute for Manufacturing Technology, TU Dresden, George-Bähr-Straße 3c, 01069 Dresden, Germany.
Denise
D
Langheinrich
Laboratory of Microbial Biofilms, Instituto de Agrobiotecnología, Universidad Pública de Navarra-CSIC-Gobierno de Navarra, Campus de Arrosadía, Pamplona, Spain.
Carmen
C
Gil
Laboratory of Microbial Biofilms, Instituto de Agrobiotecnología, Universidad Pública de Navarra-CSIC-Gobierno de Navarra, Campus de Arrosadía, Pamplona, Spain.
Cristina
C
Solano
Laboratory of Microbial Biofilms, Instituto de Agrobiotecnología, Universidad Pública de Navarra-CSIC-Gobierno de Navarra, Campus de Arrosadía, Pamplona, Spain.
Alejandro
A
Toledo-Arana
Leibniz Institute of Polymer Research (IPF) Dresden, HoheStraße 6, 01069 Dresden, Germany.
Ralf
R
Helbig
Fraunhofer Institute for Material and Beam Technology (IWS) Dresden, Winterbergstraße 28, 01277 Dresden, Germany.|Institute for Manufacturing Technology, TU Dresden, George-Bähr-Straße 3c, 01069 Dresden, Germany.
Andrés
A
Lasagni
Laboratory of Microbial Biofilms, Instituto de Agrobiotecnología, Universidad Pública de Navarra-CSIC-Gobierno de Navarra, Campus de Arrosadía, Pamplona, Spain. ilasa@unavarra.es.
Iñigo
I
Lasa
D001422:Bacterial Adhesion / Q000187:drug effects*; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects*; D057219:Biofouling; D001798:Blood Proteins / Q000737:chemistry; D006801:Humans; D007834:Lasers; D011137:Polystyrenes / Q000737:chemistry* / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000737:chemistry / Q000187:drug effects* / Q000472:pathogenicity; D013499:Surface Properties
D001672:Biocompatible Materials; D001798:Blood Proteins; D011137:Polystyrenes
Staphylococcus aureus; adhesion; biofilm; biomaterial-associated infection; direct laser interference patterning