Evaluation of the In Vitro Efficacy of Artemisia annua, Rumex abyssinicus, and Catha edulis Forsk Extracts in Cancer and Trypanosoma brucei Cells.
The current drugs against sleeping sickness are derived from cancer chemotherapeutic approaches. Herein, we aimed at evaluating the in vitro effect of alcoholic extracts of Artemisia annua (AMR), Rumex abyssinicus (RMA), and Catha edulis Forsk (CEF) on proliferation/viability of 1321N1 astrocytoma, MCF-7 breast cancer, THP-1 leukemia, and LNCaP, Du-145, and PC-3 prostate cancer cells and on Trypanosoma brucei cells. Proliferation of tumor cells was evaluated by WST-1 assay and viability/behaviour of T. brucei by cell counting and light microscopy. CEF was the most efficient growth inhibitor in comparison to AMR and RMA. Nevertheless, in LNCaP and THP-1 cells, all extracts significantly inhibited tumor growth at 3 μg/mL. All extracts inhibited proliferation of T. brucei cells in a concentration-dependent manner. Microscopic analysis revealed that 95% of the T. brucei cells died when exposed to 33 μg/mL CEF for 3 hrs. Similar results were obtained using 33 μg/mL AMR for 6 hrs. In case of RMA, however, higher concentrations were necessary to obtain similar effects on T. brucei. This demonstrates the antitumor efficacy of these extracts as well as their ability to dampen viability and proliferation of T. brucei, suggesting a common mechanism of action on highly proliferative cells, most probably by targeting cell metabolism.
Institute of Biochemistry, Faculty of Medicine, University of Leipzig, Johannisallee 30, 04103 Leipzig, Germany ; Institute of Public Health, College of Medicine and Health Sciences, University of Gondar, P.O. Box 198, Gondar, Ethiopia.
Netsanet
0000-0003-2417-5879
N
Worku
Physiology Department, Medical Faculty, Jimma University, P.O. Box 378, Jimma, Ethiopia.
Andualem
A
Mossie
Department of Tropical Medicine, Medical Mission Institute, Salvatorstrasse 7, 97067 Würzburg, Germany.
August
A
Stich
Department of Veterinary Parasitology, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 35, 04103 Leipzig, Germany.
Arwid
A
Daugschies
Institute of Biochemistry, Faculty of Medicine, University of Leipzig, Johannisallee 30, 04103 Leipzig, Germany.
Susanne
0000-0003-4289-3727
S
Trettner
Institute of Biochemistry, Faculty of Medicine, University of Leipzig, Johannisallee 30, 04103 Leipzig, Germany ; Department of Zoology, Faculty of Science, University of Alexandria, Maharram Bey, Alexandria 21511, Egypt.
Nasr Y A
0000-0002-6748-0649
NY
Hemdan
Institute of Biochemistry, Faculty of Medicine, University of Leipzig, Johannisallee 30, 04103 Leipzig, Germany.
Gerd
0000-0001-7805-9924
G
Birkenmeier