Effect of cinnamon (Cinnamomum zeylanicum) bark oil on heat stress-induced changes in sperm production, testicular lipid peroxidation, testicular apoptosis, and androgenic receptor density in developing Japanese quails.
The aim of this study was to investigate the effect of cinnamon bark oil (CBO) on heat stress (HS)-induced changes in sperm production, testicular lipid peroxidation, testicular apoptosis, and androgenic receptor (AR) density in developing Japanese quails. Fifteen-day-old 90 male chicks were assigned to two main groups. The first group (45 chicks) was kept in a thermoneutral room at 22 °C for 24 h/day. The second group (45 chicks) was kept in a room with high ambient temperature at 34 °C for 8 h/day (from 9 AM-5 PM) and at 22 °C for 16 h/day. Each of these two main groups was then divided into three subgroups (CBO groups 0, 250, 500 ppm) consisting of 15 chicks (six treatment groups in 2 × 3 factorial order). Each of subgroups was replicated for three times and each replicate included five chicks. Heat stress caused significant decreases in body weight, spermatid and testicular sperm numbers, the density of testicular Bcl-2 (antiapoptotic marker) and AR immunopositivity, and significant increases in testicular lipid peroxidation level, the density of testicular Bax (apoptotic marker) immunopositivity, and a Bax/Bcl-2 ratio along with some histopathologic damages. However, 250 and 500 ppm CBO supplementation provided significant improvements in HS-induced increased level of testicular lipid peroxidation, decreased number of spermatid and testicular sperm, decreased densities of Bcl-2 and AR immunopositivity, and some deteriorated testicular histopathologic lesions. In addition, although HS did not significantly affect the testicular glutathione level, addition of both 250 and 500 ppm CBO to diet of quails reared in both HS and thermoneutral conditions caused a significant increase when compared with quails without any consumption of CBO. In conclusion, HS-induced lipid peroxidation causes testicular damage in developing male Japanese quails and, consumption of CBO, which has antiperoxidative effect, protects their testes against HS.
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Fırat University, Elazığ, Turkey. Electronic address: gturk@firat.edu.tr.
Gaffari
G
Türk
Department of Animal Science, Faculty of Veterinary Medicine, Fırat University, Elazığ, Turkey.
Ülkü G
ÜG
Şimşek
Department of Pathology, Faculty of Veterinary Medicine, Fırat University, Elazığ, Turkey.
Ali O
AO
Çeribaşı
Department of Pathology, Faculty of Veterinary Medicine, Fırat University, Elazığ, Turkey.
Songül
S
Çeribaşı
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Fırat University, Elazığ, Turkey.
Şeyma
Ş
Özer Kaya
Department of Physiology, Faculty of Veterinary Medicine, Fırat University, Elazığ, Turkey.
Mehmet
M
Güvenç
Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary Medicine, Fırat University, Elazığ, Turkey.
Mehmet
M
Çiftçi
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Fırat University, Elazığ, Turkey.
Mustafa
M
Sönmez
Department of Physiology, Faculty of Veterinary Medicine, Fırat University, Elazığ, Turkey.
Abdurrauf
A
Yüce
Department of Histology and Embryology, Faculty of Veterinary Medicine, Fırat University, Elazığ, Turkey.
Ali
A
Bayrakdar
Department of Histology and Embryology, Faculty of Veterinary Medicine, Fırat University, Elazığ, Turkey.
Mine
M
Yaman
Veterinary Control Institute, Elazığ, Turkey.
Fadime
F
Tonbak
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D003370:Coturnix / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D018869:Heat-Shock Response / Q000187:drug effects*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D009822:Oils, Volatile / Q000494:pharmacology*; D009929:Organ Size / Q000187:drug effects; D011944:Receptors, Androgen / Q000378:metabolism*; D013076:Sperm Count; D013091:Spermatogenesis / Q000187:drug effects*; D013737:Testis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology
D009822:Oils, Volatile; D011944:Receptors, Androgen; C510046:cinnamon oil, bark
Androgenic receptor; Apoptosis; Cinnamon; Heat stress; Lipid peroxidation; Quail