[Effects of morphology of the microgroove titanium surface on expression of vinculin in human gingival fibroblasts].
OBJECTIVE
To investigate the effects of morphology of the microgroove surface on the expression of vinculin in human gingival fibroblasts, with the hope of providing basis for size selection of microgroove for transmucosal part of dental implants.


METHODS
Microgroove titanium surfaces were fabricated by photolithography with parallel grooves: 15 μm, 30 μm or 60 μm in width and 5 μm or 10 μm in depth. The groups that used different microgroove surfaces were denoted as T15/5, T15/10, T30/5, T30/10, T60/5, and T60/10. Smooth titanium surfaces (T0) were used as controls. Surface topography was detected. HGFs were cultured on the microgroove surfaces. Morphology of vinculin was analyzed using CLSM. Expression level of vinculin was compared among different groups by real-time PCR and Western blotting. SPSS17.0 software package was used for statistical analysis.


RESULTS
Immunofluorescence staining revealed increase of green vinculin fluorescent spots as the increase of microgroove width, while T60 group had the highest mRNA and protein expression and T15 group had the lowest. Microgroove depth had no effect on the expression of vinculin under the same width.


CONCLUSIONS
Morphology of microgroove surface has effect on the expression of vinculin, and the morphology of microgroove surface in T60 group is the best size for the transmucosal part in terms of expression of vinculin protein expression.
Department of Oral Medicine, Xiamen Medical College. Xiamen 361000;Fujian Province,China.E-mail:dentistyz@126.com.
Ying-zhen
YZ
Lai
Jiang
J
Chen
Shan
S
Lin
D002448:Cell Adhesion; D015921:Dental Implants; D005347:Fibroblasts; D005881:Gingiva; D006801:Humans; D013499:Surface Properties; D014025:Titanium; D016596:Vinculin
D015921:Dental Implants; D016596:Vinculin; D014025:Titanium