Antiviral Effects of Novel Herbal Medicine KIOM-C, on Diverse Viruses.
In order to identify new potential antiviral agents, recent studies have advocated thorough testing of herbal medicines or natural substances that are traditionally used to prevent viral infections. Antiviral activities and the mechanism of action of the total aqueous extract preparation of KIOM-C, a novel herbal medicine, against diverse types of viruses were investigated. In vitro antiviral activity against A/Puerto Rico/8/34 (H1N1) (PR8), vesicular stomatitis virus (VSV), and Newcastle disease virus (NDV) through the induction of type-I interferon related protein phosphorylation and up-regulation of pro-inflammatory cytokines in murine macrophage cells (RAW264.7) were determined. In vivo, KIOM-C-treated BALB/c mice showed higher survivability and lower lung viral titers when challenged with A/Aquatic bird/Korea/W81/2005 (H5N2), A/PR/8/34(H1N1), A/Aquatic bird/Korea/W44/2005(H7N3) or A/Chicken/Korea/116 /2004(H9N2) influenza subtypes in contrast with the non-treated group. The present study revealed that total aqueous extract preparation of KIOM-C stimulates an antiviral state in murine macrophage cells and in mice leading to inhibition of viral infection and protection against lethal challenges.
College of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea; College of Veterinary Medicine and Biomedical Sciences, Cavite State University, Cavite, Philippines.
Melbourne R
MR
Talactac
College of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea; Faculty of Veterinary Medicine, Chittagong Veterinary and Animal Sciences University, Chittagong, Bangladesh.
Mohammed Y E
MY
Chowdhury
College of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea.
Min-Eun
ME
Park
College of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea.
Prasanna
P
Weeratunga
College of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea.
Tae-Hwan
TH
Kim
Korean Medicine (KM) Based Herbal Drug Development Group, Korea Institute of Oriental Medicine, Deajeon, and Republic of Korea.
Won-Kyung
WK
Cho
College of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea.
Chul-Joong
CJ
Kim
Korean Medicine (KM) Based Herbal Drug Development Group, Korea Institute of Oriental Medicine, Deajeon, and Republic of Korea.
Jin Yeul
JY
Ma
College of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea.
Jong-Soo
JS
Lee
D000818:Animals; D000998:Antiviral Agents / Q000008:administration & dosage / Q000494:pharmacology*; D002460:Cell Line; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D004195:Disease Models, Animal; D005260:Female; D029001:Herbal Medicine; D018836:Inflammation Mediators / Q000378:metabolism; D007370:Interferon Type I / Q000378:metabolism; D008168:Lung / Q000473:pathology / Q000821:virology; D051379:Mice; D009976:Orthomyxoviridae Infections / Q000188:drug therapy / Q000401:mortality / Q000821:virology; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects; D014780:Viruses / Q000187:drug effects*
D000998:Antiviral Agents; D016207:Cytokines; D018836:Inflammation Mediators; D007370:Interferon Type I; C000591314:KIOM-C preparation; D010936:Plant Extracts