A small molecule--DNA binding landscape.
This brief account traces the development of a "competition dialysis" method used to characterize the structural and sequence selectivity of DNA binding compounds. The method was inspired by a simple "differential dialysis" method pioneered by Don Crothers to explore base-selective intercalator binding. Results from compiled competition dialysis studies provide a small-molecule DNA binding landscape that shows a rich diversity of interactions and molecular recognition.
Department of Medicine, James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202.
Jonathan B
JB
Chaires
D001665:Binding Sites; D004247:DNA / Q000737:chemistry*; D009690:Nucleic Acid Conformation
D004247:DNA
DNA binding; Donald Crothers; dialysis; molecular recognition