Complement system and immunological mediators: Their involvements in the induced inflammatory process by Androctonus australis hector venom and its toxic components.
Androctonus australis hector scorpion venom is well known by its high toxicity, it induces massive release of neurotransmitters that lead to pathophysiological disorders in cardiovascular, neuro-hormonal and immune systems. Previous studies have shown the relationship between the severity of scorpion envenoming and immune system activation. This study was assessed to investigate the involvement of complement system and inflammatory mediators after sublethal injection of Aah venom, its toxic fraction (FtoxG50) and its main toxins (AahI and AahII) into NMRI mice. The Activation complement system by the venom is also compared to that induced of lipopolysaccharides (LPS). Obtained results showed that seric complement system (CS) is activated by the venom and by its toxic components; this activation is more pronounced into liver tissue when toxic components (FtoxG50, AahI or AahII) are used. Increase of cytokine levels (IL1β, TNFα and ICAM) into hepatic tissue induced by AahI or AahII neurotoxins is correlated with tissue alterations. Aprotinin, a non specific inhibitor of complement system seems to be able to reduce CS consumption and to restore partially the induced tissue damage by venom. The mechanisms by which toxic fraction or LPS induced the activation of complement system seem to be different. Sensitivity of hepatic tissue is more pronounced after FtoxG50 injection; however lung tissue is more sensible to LPS than FoxG50.
USTHB, Faculty of Biological Sciences, Laboratory of Cellular and Molecular Biology, BP 32 El Alia, Bab Ezzouar, Algiers, Algeria.
Nadjia
N
Bekkari
Aix-Marseille University, CNRS UMR 7286 CRN2M, IFR Jean-Roche, Faculté de Médecine Nord, Bd Pierre Dramard, 13916 Marseille, Cedex 20, France.
Marie-France
MF
Martin-Eauclaire
USTHB, Faculty of Biological Sciences, Laboratory of Cellular and Molecular Biology, BP 32 El Alia, Bab Ezzouar, Algiers, Algeria. Electronic address: flaraba@hotmail.com.
Fatima
F
Laraba-Djebari
D000818:Animals; D003167:Complement Activation / Q000502:physiology*; D007249:Inflammation / Q000276:immunology*; D051379:Mice; D012604:Scorpion Venoms / Q000009:adverse effects*; D012605:Scorpions
D012604:Scorpion Venoms
Aah venom; AahI; AahII; Complement system; Cytotoxicity; Ftox G50