Metronidazole- and carbapenem-resistant bacteroides thetaiotaomicron isolated in Rochester, Minnesota, in 2014.
Emerging antimicrobial resistance in members of the Bacteroides fragilis group is a concern in clinical medicine. Although metronidazole and carbapenem resistance have been reported in Bacteroides thetaiotaomicron, a member of the B. fragilis group, they have not, to the best of our knowledge, been reported together in the same B. thetaiotaomicron isolate. Herein, we report isolation of piperacillin-tazobactam-, metronidazole-, clindamycin-, ertapenem-, and meropenem-resistant B. thetaiotaomicron from a patient with postoperative intra-abdominal abscess and empyema. Whole-genome sequencing demonstrated the presence of nimD with at least a portion of IS1169 upstream, a second putative nim gene, two β-lactamase genes (one of which has not been previously reported), two tetX genes, tetQ, ermF, two cat genes, and a number of efflux pumps. This report highlights emerging antimicrobial resistance in B. thetaiotaomicron and the importance of identification and antimicrobial susceptibility testing of selected anaerobic bacteria.
Division of Infectious Diseases, Mayo Clinic, Rochester, Minnesota, USA.
Sapna P
SP
Sadarangani
Division of Clinical Microbiology, Mayo Clinic, Rochester, Minnesota, USA.
Scott A
SA
Cunningham
Department of Surgery, Mayo Clinic, Rochester, Minnesota, USA Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
Patricio R
http://orcid.org/0000-0003-0282-4217
PR
Jeraldo
Division of Infectious Diseases, Mayo Clinic, Rochester, Minnesota, USA.
John W
JW
Wilson
Division of Clinical Microbiology, Mayo Clinic, Rochester, Minnesota, USA.
Reeti
R
Khare
Division of Infectious Diseases, Mayo Clinic, Rochester, Minnesota, USA Division of Clinical Microbiology, Mayo Clinic, Rochester, Minnesota, USA patel.robin@mayo.edu.
Robin
R
Patel
D000038:Abscess / Q000382:microbiology; D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics; D001439:Bacteroides / Q000187:drug effects*; D001442:Bacteroides Infections / Q000382:microbiology*; D015780:Carbapenems / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000235:genetics; D004239:Diverticulitis, Colonic / Q000601:surgery; D024881:Drug Resistance, Bacterial; D004653:Empyema / Q000382:microbiology; D016680:Genome, Bacterial / Q000235:genetics; D006801:Humans; D008297:Male; D008795:Metronidazole / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D008910:Minnesota; D013530:Surgical Wound Infection / Q000382:microbiology; D001618:beta-Lactamases / Q000235:genetics
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D004269:DNA, Bacterial; C094010:NimD protein, Bacteroides fragilis; D008795:Metronidazole; D001618:beta-Lactamases