Determination of the potential illegal addition of β-blockers to function foods by QuEChERS sample preparation and UPLC-MS/MS analysis.
A novel method was established for the determination of 11 β-blockers in sedative functional foods using QuEchERS sample preparation coupled with UPLC-MS/MS. The analytes were extracted twice with acetic acid-acetonitrile-methanol (0.1:3:7, v/v/v), and the extracts were purified with mixed QuEchERS adsorbents. Separation was performed on a C18 column, and detection using electrospray ionisation in positive-ion mode. The linear range for the 11 analytes was from 1 to 500 μg l(-1), and the correlation coefficients were above 0.9990. The limits of detection and quantification were 0.02-0.16 and 0.07-0.54 μg kg(-1), respectively. The average recovery for 11 analytes at the three spiking levels varied from 71.8% to 121.9%, and the relative standard deviation was 2.4-12.6%. The method was applied to the analysis of β-blockers in 22 real samples, but none of the analytes was detected. The proposed method is sensitive, efficient, reliable and applicable to real samples.
a College of Pharmacy , Chongqing Medical University , Chongqing , China.
Jingchuan
J
Shang
Xiaoqin
X
He
Cunxian
C
Xi
Bobin
B
Tang
Guomin
G
Wang
Dongdong
D
Chen
Tao
T
Peng
Zhaode
Z
Mu
D000319:Adrenergic beta-Antagonists / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D055951:Functional Food / Q000032:analysis*; D015394:Molecular Structure; D013048:Specimen Handling; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000319:Adrenergic beta-Antagonists
QuEchERS; UPLC-MS/MS; sedative function foods; β-blockers