The potential of interleukin 12 receptor beta 2 (IL12RB2) and tumor necrosis factor receptor superfamily member 8 (TNFRSF8) gene as diagnostic biomarkers of oral lichen planus (OLP).
OBJECTIVE
This study evaluated the potential of interleukin 12 receptor beta 2 and tumor necrosis factor receptor superfamily member 8 as diagnostic biomarkers of oral lichen planus (OLP).


METHODS
The mRNA expression of IL12RB2 and TNFRSF8 in FFPE OLP samples (OLP group, n = 38) were investigated with quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) analysis and compared to those of chronic non-specific mucositis (Non-OLP group, n = 25) and normal mucosa (Normal group, n = 18). Predictive modeling of the expression of IL12RB2 and TNFRSF8 was constructed using support vector machine (SVM), random forest (RF), linear discriminant analysis (LDA), neural network (NN) and naive Bayes (NB) methods.


RESULTS
Normalized expression of IL12RB2 in the OLP group (3.78 ± 1.67) was significantly higher than the Normal group (1.97 ± 1.12), but lower than the Non-OLP group (6.86 ± 1.67). TNFRSF8 gene expression in the OLP group (7.46 ± 1.51) was significantly higher than the Normal group (2.90 ± 1.61), but no significant difference was found between the OLP and Non-OLP groups. The ratio of IL12RB2/TNFRSF8 in the OLP group (0.52 ± 0.23) was significantly lower than the Normal group (0.74 ± 0.39) and the Non-OLP group (1.07 ± 0.38). In the predictive modeling, the area under receiver operating characteristic (ROC) curves (AUC) ranged from 0.83-0.92 and their accuracy was higher than 0.75 in all methods.


CONCLUSIONS
The IL12RB2/TNFRSF8 ratio can be a useful diagnostic tool for OLP.
Department of Oral and Maxillofacial Surgery, School of Dentistry.
Seung-Ho
SH
Jeon
Eun-Hyoung
EH
Jeon
Jin-Yong
JY
Lee
Yeon-Sun
YS
Kim
Hye-Jung
HJ
Yoon
Sam-Pyo
SP
Hong
Jong-Ho
JH
Lee
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019540:Area Under Curve; D001499:Bayes Theorem; D015415:Biomarkers / Q000032:analysis; D016002:Discriminant Analysis; D005260:Female; D005544:Forecasting; D006801:Humans; D017730:Ki-1 Antigen / Q000032:analysis*; D017676:Lichen Planus, Oral / Q000378:metabolism* / Q000473:pathology; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000737:chemistry / Q000473:pathology; D016571:Neural Networks, Computer; D012372:ROC Curve; D053707:Receptors, Interleukin-12 / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013280:Stomatitis / Q000378:metabolism / Q000473:pathology; D055815:Young Adult
D015415:Biomarkers; C508439:IL12RB2 protein, human; D017730:Ki-1 Antigen; D053707:Receptors, Interleukin-12
IL12RB2; TNFRSF8; biomarker; formalin-fixed; oral lichen planus (OLP); paraffin-embedded (FFPE); predictive modeling