Backbone, side chain and heme resonance assignments of cytochrome OmcF from Geobacter sulfurreducens.
Gene knockout studies on Geobacter sulfurreducens (Gs) cells showed that the outer membrane cytochrome OmcF is involved in respiratory pathways leading to the extracellular reduction of Fe(III) citrate and U(VI) oxide. In addition, microarray analysis of OmcF-deficient mutant versus the wild-type strain revealed that many of the genes with decreased transcript level were those whose expression is upregulated in cells grown with a graphite electrode as electron acceptor. This suggests that OmcF also regulates the electron transfer to electrode surfaces and the concomitant electrical current production by Gs in microbial fuel cells. Extracellular electron transfer processes (EET) constitute nowadays the foundations to develop biotechnological applications in biofuel production, bioremediation and bioenergy. Therefore, the structural characterization of OmcF is a fundamental step to understand the mechanisms underlying EET. Here, we report the complete assignment of the heme proton signals together with (1)H, (13)C and (15)N backbone and side chain assignments of the OmcF, excluding the hydrophobic residues of the N-terminal predicted lipid anchor.
UCIBIO, REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Campus Caparica, 2829-516, Caparica, Portugal.
Joana M
JM
Dantas
UCIBIO, REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Campus Caparica, 2829-516, Caparica, Portugal.
Marta
M
Silva E Sousa
UCIBIO, REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Campus Caparica, 2829-516, Caparica, Portugal. csalgueiro@fct.unl.pt.
Carlos A
CA
Salgueiro
Departamento de Química Física Biológica, Instituto de Química-Física "Rocasolano", CSIC, Serrano 119, 28006, Madrid, Spain. mbruix@iqfr.csic.es.
Marta
M
Bruix
D001426:Bacterial Proteins / Q000737:chemistry*; D003580:Cytochromes / Q000737:chemistry*; D045302:Geobacter / Q000378:metabolism*; D006418:Heme / Q000737:chemistry*; D009587:Nitrogen Isotopes; D017433:Protein Structure, Secondary; D066244:Proton Magnetic Resonance Spectroscopy
D001426:Bacterial Proteins; D003580:Cytochromes; D009587:Nitrogen Isotopes; D006418:Heme
Geobacter sulfurreducens; NMR; OmcF; cytochrome