Detection of QTLs for salt tolerance in Asian barley (Hordeum vulgare L.) by association analysis with SNP markers.
Two hundred ninety-six Asian barley (Hordeum vulgare L.) accessions were assessed to detect QTLs underlying salt tolerance by association analysis using a 384 single nucleotide polymorphism (SNP) marker system. The experiment was laid out at the seedling stage in a hydroponic solution under control and 250 mM NaCl solution with three replications of four plants each. Salt tolerance was assessed by leaf injury score (LIS) and salt tolerance indices (STIs) of the number of leaves (NL), shoot length (SL), root length (RL), shoot dry weight (SDW) and root dry weight (RDW). LIS was scored from 1 to 5 according to the severity of necrosis and chlorosis observed on leaves. There was a wide variation in salt tolerance among Asian barley accessions. LIS and STI (SDW) were the most suitable traits for screening salt tolerance. Association was estimated between markers and traits to detect QTLs for LIS and STI (SDW). Seven significant QTLs were located on chromosomes 1H (2 QTLs), 2H (2 QTLs), 3H (1 QTL), 4H (1 QTL) and 5H (1 QTL). Five QTLs were associated with LIS and 2 QTLs with STI (SDW). Two QTLs associated with LIS were newly identified on chromosomes 3H and 4H.
Graduate School of Life and Environmental Sciences, University of Tsukuba , Tennodai 1-1-1, Tsukuba, Ibaraki 305-8572 , Japan.
Hanen
H
Sbei
Institute of Plant Science and Resources, Okayama University , Chuo 2-20-1, Kurashiki, Okayama 710-0046 , Japan.
Kazuhiro
K
Sato
Graduate School of Life and Environmental Sciences, University of Tsukuba , Tennodai 1-1-1, Tsukuba, Ibaraki 305-8572 , Japan ; The Alliance for Research on North Africa, University of Tsukuba , Tennodai 1-1-1, Tsukuba, Ibaraki 305-8572 , Japan.
Tariq
T
Shehzad
National Institute of Agriculture at Tunis , 43 Avenue Charles Nicolle, Mahrajene City, 1082 Tunisia.
Moncef
M
Harrabi
The Alliance for Research on North Africa, University of Tsukuba , Tennodai 1-1-1, Tsukuba, Ibaraki 305-8572 , Japan.
Kazutoshi
K
Okuno
Hordeum vulgare L.; SNP markers; association mapping; leaf injury score; quantitative trait loci; salt tolerance; salt tolerance index