[CecropinA-magainin, a new hybrid antibacterial peptide against meticillin-resistant Staphylococcus aureus].
OBJECTIVE
To study the mechanism of cecropinA-mangainin treatment on the meticillin-resistant Staphylococcus anreus biofilms.


METHODS
The activity of the hybrid antibacterial peptide against Staphylococcus anreus was evaluated by the minimum inhibitory concentration (MIC) test and its effect on the bacteria membrane changes were observed through transmission electron microscope. The concentration of K+ of the tested bacterial liquid after interact with antibacterial peptide was detected with atomic absorption spectrometer. The changes of the treated bacteria biofilm was also evaluated by using flow cytometry.


RESULTS
The results demonstrated that the MIC of the peptide against Staphylococcus aureus was 64 microg/mL. The ultrastructure changes of the meticillin-resistant Staphylococcus anreus membrane and the rising concentration of intracellular K+ were observed. And increased number of PI positive cells was also observed after hybrid antibacterial peptide treatmennt.


CONCLUSIONS
The hybrid antibacterial peptide could kill the meticillin-resistant Staphylococcus anreus by damage the treated bacteria membrane.
Long-mei
LM
Yu
Tian-tian
TT
Yang
Xiu-qing
XQ
Wang
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects; D054806:Magainins / Q000494:pharmacology*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects*; D008826:Microbial Sensitivity Tests
D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D054806:Magainins; C031161:cecropin A