Day-3 embryo metabolomics in the spent culture media is altered in obese women undergoing in vitro fertilization.
OBJECTIVE
To determine whether the global metabolomic profile of the spent culture media (SCM) of day-3 embryos is different in obese and normoweight women undergoing in vitro fertilization (IVF).


METHODS
Prospective cohort analysis.


METHODS
IVF clinic.


METHODS
Twenty-eight young, nonsmoking women with normoweight, nonsmoking male partners with mild/normal sperm factors undergoing a first IVF attempt for idiopathic infertility, tubal factor infertility, or failed ovulation induction: obese ovulatory women (n = 12); obese women with polycystic ovary syndrome (PCOS; n = 4); normoweight ovulatory women (n = 12).


METHODS
Fifty μl of SCM collected from two day-3 embryos of each cohort.


METHODS
Metabolomic profiling via ultrahigh performance liquid chromatography coupled to mass spectrometry of SCM from a total of 56 embryos.


RESULTS
The untargeted metabolomic profile was different in obese and normoweight women. Partial least squares discriminant analysis resulted in a clear separation of samples when a total of 551 differential metabolites were considered. A prediction model was generated using the most consistent metabolites. Most of the metabolites identified were saturated fatty acids, which were detected in lower concentrations in the SCM of embryos from obese women. The metabolomic profile was similar in obese women with or without PCOS.


CONCLUSIONS
The metabolomic profile in the SCM of day-3 embryos is different in normoweight and obese women. Saturated fatty acids seem to be reduced when embryos from obese patients are present.


BACKGROUND
NCT01448863.
Instituto Valenciano de Infertilidad, University of Valencia, Valencia, Spain; Department of Pediatrics, Obstetrics and Gynecology, Faculty of Medicine, University of Valencia, Valencia, Spain; IVI Foundation, Valencia, Spain. Electronic address: jose.bellver@ivi.es.
José
J
Bellver
Instituto Valenciano de Infertilidad, University of Valencia, Valencia, Spain.
María J
MJ
De Los Santos
Instituto Valenciano de Infertilidad, University of Valencia, Valencia, Spain.
Pilar
P
Alamá
Instituto Valenciano de Infertilidad, University of Valencia, Valencia, Spain.
Damià
D
Castelló
Instituto Valenciano de Infertilidad, University of Valencia, Valencia, Spain.
Laura
L
Privitera
Instituto Valenciano de Infertilidad, University of Valencia, Valencia, Spain.
Daniela
D
Galliano
Instituto Valenciano de Infertilidad, University of Valencia, Valencia, Spain.
Elena
E
Labarta
Instituto Valenciano de Infertilidad, University of Valencia, Valencia, Spain.
Carmen
C
Vidal
Instituto Valenciano de Infertilidad, University of Valencia, Valencia, Spain; Department of Pediatrics, Obstetrics and Gynecology, Faculty of Medicine, University of Valencia, Valencia, Spain; IVI Foundation, Valencia, Spain.
Antonio
A
Pellicer
IVI Foundation, Valencia, Spain; INCLIVA Biomedical Research Institute, Valencia, Spain.
Francisco
F
Domínguez
D000328:Adult; D003470:Culture Media / Q000378:metabolism; D046149:Embryo Culture Techniques / Q000379:methods; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000378:metabolism*; D005260:Female; D005307:Fertilization in Vitro; D005786:Gene Expression Regulation; D006801:Humans; D007247:Infertility, Female / Q000150:complications / Q000378:metabolism* / Q000628:therapy*; D008297:Male; D055442:Metabolome; D009765:Obesity / Q000150:complications / Q000378:metabolism*; D011085:Polycystic Ovary Syndrome / Q000378:metabolism / Q000628:therapy; D020543:Proteome / Q000378:metabolism*
D003470:Culture Media; D020543:Proteome
Day-3 embryos; female obesity; metabolomics; saturated fatty acids; spent culture media