Stem cell behavior on tailored porous oxide surface coatings.
Nanoscale surface topographies are known to have a profound influence on cell behavior, including cell guidance, migration, morphology, proliferation, and differentiation. In this study, we have observed the behavior of human mesenchymal stem cells cultured on a range of tailored porous SiO2 and TiO2 nanostructured surface coatings fabricated via glancing angle electron-beam deposition. By controlling the physical vapor deposition angle during fabrication, we could control systematically the deposited coating porosity, along with associated topographic features. Immunocytochemistry and image analysis quantitatively revealed the number of adherent cells, as well as their basic cellular morphology, on these surfaces. Signaling pathway studies showed that even with subtle changes in nanoscale surface structures, the behavior of mesenchymal stem cells was strongly influenced by the precise surface structures of these porous coatings.
Rensselaer Nanotechnology Center, Rensselear Polytechnic Institute, Troy, NY 12180, United States. Electronic address: samla234@yahoo.fr.
Sandrine
S
Lavenus
Rensselaer Nanotechnology Center, Rensselear Polytechnic Institute, Troy, NY 12180, United States.
David J
DJ
Poxson
Rensselaer Nanotechnology Center, Rensselear Polytechnic Institute, Troy, NY 12180, United States; Department of Materials Science and Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180, United States; Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, United States.
Nika
N
Ogievetsky
Rensselaer Nanotechnology Center, Rensselear Polytechnic Institute, Troy, NY 12180, United States; Department of Materials Science and Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180, United States; Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180, United States; Department of Biology, Rensselaer Polytechnic Institute, Troy, NY 12180, United States; Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, United States.
Jonathan S
JS
Dordick
Rensselaer Nanotechnology Center, Rensselear Polytechnic Institute, Troy, NY 12180, United States; Department of Materials Science and Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180, United States; Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, United States.
Richard W
RW
Siegel
D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000295:instrumentation*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002469:Cell Separation; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D049329:Nanostructures / Q000737:chemistry; D016062:Porosity; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
D001672:Biocompatible Materials; C009495:titanium dioxide; D012822:Silicon Dioxide; D014025:Titanium
GLAD; Mesenchymal stem cells; Nanotopography; Porosity; Silicon dioxide; Titanium dioxide