Isolation and characterization of 13 polymorphic microsatellite loci in Hypostomus ancistroides (Teleostei, Loricariidae) and cross-amplification in related species.
This study details 13 novel polymorphic microsatellite loci in the armoured catfish Hypostomus ancistroides, and assesses their utility for population genetic studies. The analysis of 30 individuals revealed a total of 99 different alleles (ranging from two to 15 alleles per locus), with an average of 7·62 alleles per locus, with observed and expected heterozygosities ranging from 0·103 to 0·931 and from 0·102 to 0·906, respectively. One of the 13 loci showed significant deviations from Hardy-Weinberg equilibrium, probably due to the presence of null alleles, inferred from the excess of homozygotes.
Laboratório de Genética e Conservação (GECON), Universidade Estadual do Norte Paraná, Rua Portugal, 340, Cornélio Procópio, PR 86300-000, Brazil.
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BA
Galindo
Departamento de Biologia Geral, Universidade Estadual de Londrina, Rod. Celso Garcia Cid, km 380, Londrina, PR 86051-980, Brazil.
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Ferreira
Departamento de Biologia Geral, Universidade Estadual de Londrina, Rod. Celso Garcia Cid, km 380, Londrina, PR 86051-980, Brazil.
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Almeida
School of Biology and Environmental Science, UCD Science Education and Research Centre - West, University College Dublin, Dublin 4, Ireland.
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J
Carlsson
Departamento de Biologia Geral, Universidade Estadual de Londrina, Rod. Celso Garcia Cid, km 380, Londrina, PR 86051-980, Brazil.
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SH
Sofia
D000483:Alleles; D000818:Animals; D001938:Brazil; D002397:Catfishes / Q000235:genetics*; D005819:Genetic Markers; D006579:Heterozygote; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
D005819:Genetic Markers
Hypostominae; armoured catfishes; genetic marker; neotropical fish; population genetics