[Identification of aminoalcohol-diterpenoid alkaloids in Aconiti Lateralis Radix Praeparata and study of their cardiac effects].
In order to affirm the cardioactive components in Fuzi, we identified a group of aminoalcohol- diterpenoid alkaloids in Fuzi using ultra high-performance liquid chromatography coupled with electrospray ionization mass spectrometer (UPLC-ESI-MS) method. Among a total of forty-one isolated ingredients, thirteen major aminoalcohol-diterpenoid alkaloids were identified by comparing their retention times and MS spectra with those of the reference substances. Moreover, Fuzi samples from different places of origin and with different processing methods were examined and their components displayed a pattern of high similarity, though the relative abundance varies probably due to their different processing methods. Furthermore, the cardiac effect of each identified alkaloid was individually evaluated using the isolated bullfrog heart perfusion experiment. Among the thirteen aminoalcohol diterpenoid alkaloids tested, six of them significantly enhanced the amplitude rates. Taken together, we affirm that the cardioactive components in Fuzi are aminoalcohol-diterpenoid alkaloids, shedding light on future studies of the mechanisms and development of these cardioactive compounds.
Lu
L
Wang
Jia-Yu
JY
Ding
Xiu-Xiu
XX
Liu
Ming-Hai
MH
Tang
Ruo-Bing
RB
Chao
Feng-Peng
FP
Wang
D031841:Aconitum / Q000737:chemistry*; D000470:Alkaloids / Q000737:chemistry*; D000605:Amino Alcohols / Q000737:chemistry; D000818:Animals; D002316:Cardiotonic Agents / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D006321:Heart / Q000187:drug effects*; D066298:In Vitro Techniques; D010936:Plant Extracts / Q000737:chemistry*; D011892:Rana catesbeiana; D021241:Spectrometry, Mass, Electrospray Ionization
D000470:Alkaloids; D000605:Amino Alcohols; D002316:Cardiotonic Agents; D004224:Diterpenes; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; C575009:fuzi drug herbal