Identification and Characterization of a PRDM14 Homolog in Japanese Flounder (Paralichthys olivaceus).
PRDM14 is a PR (PRDI-BF1-RIZ1 homologous) domain protein with six zinc fingers and essential roles in genome-wide epigenetic reprogramming. This protein is required for the establishment of germ cells and the maintenance of the embryonic stem cell ground state. In this study, we cloned the full-length cDNA and genomic DNA of the Paralichthys olivaceus prdm14 (Po-prdm14) gene and isolated the 5' regulatory region of Po-prdm14 by whole-genome sequencing. Peptide sequence alignment, gene structure analysis, and phylogenetic analysis revealed that Po-PRDM14 was homologous to mammalian PRDM14. Results of real-time quantitative polymerase chain reaction amplification (RT-qPCR) and in situ hybridization (ISH) in embryos demonstrated that Po-prdm14 was highly expressed between the morula and late gastrula stages, with its expression peaking in the early gastrula stage. Relatively low expression of Po-prdm14 was observed in the other developmental stages. ISH of gonadal tissues revealed that the transcripts were located in the nucleus of the oocytes in the ovaries but only in the spermatogonia and not the spermatocytes in the testes. We also presume that the Po-prdm14 transcription factor binding sites and their conserved binding region among vertebrates. The combined results suggest that Po-PRDM14 has a conserved function in teleosts and mammals.
Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, Qingdao 266003, China. fanlin_lynne@sina.cn.
Lin
L
Fan
Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, Qingdao 266003, China. j15964239284@163.com.
Jiajun
J
Jiang
Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, Qingdao 266003, China. gjn.1127@163.com.
Jinning
J
Gao
Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, Qingdao 266003, China. song.huayu@163.com.
Huayu
H
Song
Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, Qingdao 266003, China. ljkmn911@outlook.com.
Jinxiang
J
Liu
Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, Qingdao 266003, China. yanglikun0320@126.com.
Likun
L
Yang
Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, Qingdao 266003, China. xiumei0210@163.com.
Zan
Z
Li
Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, Qingdao 266003, China. gtpeng@163.com.
Yan
Y
Chen
Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, Qingdao 266003, China. qzhang@ouc.edu.cn.
Quanqi
Q
Zhang
Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, Qingdao 266003, China. wangxubo@ouc.edu.cn.
Xubo
X
Wang
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005432:Flounder / Q000145:classification / Q000235:genetics*; D005786:Gene Expression Regulation; D023061:Gene Order; D056426:Genetic Loci; D006066:Gonads / Q000378:metabolism; D008969:Molecular Sequence Data; D059372:Nucleotide Motifs; D010802:Phylogeny; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D017385:Sequence Homology; D024363:Transcription Initiation Site
D018076:DNA, Complementary; D012097:Repressor Proteins