Sub-Ice Microalgal and Bacterial Communities in Freshwater Lake Baikal, Russia.
The sub-ice environment of Lake Baikal represents a special ecotope where strongly increasing microbial biomass causes an "ice-bloom" contributing therefore to the ecosystem functioning and global element turnover under low temperature in the world's largest freshwater lake. In this work, we analyzed bacterial and microalgal communities and their succession in the sub-ice environment in March-April 2010-2012. It was found out that two dinoflagellate species (Gymnodinium baicalense var. minor and Peridinium baicalense Kisselew et Zwetkow) and four diatom species (Aulacoseira islandica, A. baicalensis, Synedra acus subsp. radians, and Synedra ulna) predominated in the microalgal communities. Interestingly, among all microalgae, the diatom A. islandica showed the highest number of physically attached bacterial cells (up to 67 ± 16 bacteria per alga). Bacterial communities analyzed with pyrosequencing of 16S rRNA gene fragments were diverse and represented by 161 genera. Phyla Proteobacteria, Verrucomicrobia, Actinobacteria, Acidobacteria, Bacteroidetes, and Cyanobacteria represented a core community independently on microalgal composition, although the relative abundance of these bacterial phyla strongly varied across sampling sites and time points; unique OTUs from other groups were rare.
Limnological Institute, Siberian Branch of the Russian Academy of Sciences, 3, Ulan-Batorskaya St., Irkutsk, 664033, Russia. maria.bashenkhaeva@gmail.com.
Maria V
http://orcid.org/0000-0002-2551-5153
MV
Bashenkhaeva
Limnological Institute, Siberian Branch of the Russian Academy of Sciences, 3, Ulan-Batorskaya St., Irkutsk, 664033, Russia.
Yulia R
YR
Zakharova
Limnological Institute, Siberian Branch of the Russian Academy of Sciences, 3, Ulan-Batorskaya St., Irkutsk, 664033, Russia.
Darya P
DP
Petrova
Limnological Institute, Siberian Branch of the Russian Academy of Sciences, 3, Ulan-Batorskaya St., Irkutsk, 664033, Russia.
Igor V
IV
Khanaev
Limnological Institute, Siberian Branch of the Russian Academy of Sciences, 3, Ulan-Batorskaya St., Irkutsk, 664033, Russia.
Yuri P
YP
Galachyants
Limnological Institute, Siberian Branch of the Russian Academy of Sciences, 3, Ulan-Batorskaya St., Irkutsk, 664033, Russia.
Yelena V
YV
Likhoshway
D001419:Bacteria / Q000235:genetics; D018407:Bacterial Physiological Phenomena; D044822:Biodiversity; D017753:Ecosystem; D046448:Ice Cover / Q000382:microbiology; D060106:Lakes / Q000382:microbiology; D058086:Microalgae / Q000235:genetics / Q000502:physiology*; D064307:Microbiota; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012426:Russia; D012621:Seasons
D012336:RNA, Ribosomal, 16S
Bacteria; Lake Baikal; Microalgae; Pyrosequencing; Sub-ice community