Mutagenicity monitoring following battlefield exposures: Molecular analysis of HPRT mutations in Gulf War I veterans exposed to depleted uranium.
Molecular studies that involved cDNA and genomic DNA sequencing as well as multiplex PCR of the HPRT gene were performed to determine the molecular mutational spectrum for 1,377 HPRT mutant isolates obtained from 61 Veterans of the 1991 Gulf War, most of whom were exposed to depleted uranium (DU). Mutant colonies were isolated from one to four times from each Veteran (in 2003, 2005, 2007, and/or 2009). The relative frequencies of the various types of mutations (point mutations, deletions, insertions, etc.) were compared between high versus low DU exposed groups, (based on their urine U concentration levels), with HPRT mutant frequency (as determined in the companion paper) and with a database of historic controls. The mutational spectrum includes all classes of gene mutations with no significant differences observed in Veterans related to their DU exposures.
Department of Pediatrics, University of Vermont College of Medicine, Burlington, Vermont.
Janice A
JA
Nicklas
Department of Pathology, University of Vermont College of Medicine, Burlington, Vermont.|Biostatistics Unit, University of Vermont College of Medicine, Burlington, Vermont.
Richard J
RJ
Albertini
Center for Clinical and Translational Science - Biomedical Informatics Unit, University of Vermont, Burlington, Vermont.
Pamela M
PM
Vacek
Division of Newborn Medicine, Department of Pediatrics, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania.
Stephanie K
SK
Ardell
Center for Clinical and Translational Science - Biomedical Informatics Unit, University of Vermont, Burlington, Vermont.
Elizabeth W
EW
Carter
Occupational Health Program, Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland.
Melissa A
MA
McDiarmid
Department of Veterans Affairs Medical Center, Baltimore, Maryland.
Susan M
SM
Engelhardt
Occupational Health Program, Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland.
Patricia W
PW
Gucer
Occupational Health Program, Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland.
Katherine S
KS
Squibb
D000595:Amino Acid Sequence; D001483:Base Sequence; D002478:Cells, Cultured; D004252:DNA Mutational Analysis; D005787:Gene Frequency; D047830:Gulf War; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000737:chemistry / Q000235:genetics*; D008137:Longitudinal Studies; D008297:Male; D008889:Military Personnel; D008969:Molecular Sequence Data; D009153:Mutagens / Q000633:toxicity*; D009154:Mutation; D016273:Occupational Exposure; D014501:Uranium / Q000633:toxicity*
D009153:Mutagens; D014501:Uranium; D007041:Hypoxanthine Phosphoribosyltransferase
DNA sequencing; HPRT; HPRT mutations; depleted uranium