Bullera vrieseae sp. nov., a tremellaceous yeast species isolated from bromeliads.
Two independent surveys of yeasts associated with different bromeliads in different Brazilian regions led to the proposal of a novel yeast species, Bullera vrieseae sp. nov., belonging to the Tremellales clade (Agaricomycotina, Basidiomycota). Analysis of the sequences in the internal transcribed spacer (ITS) region and D1/D2 domain of the LSU rRNA gene suggested affinity to a phylogenetic lineage that includes Bullera miyagiana and Bullera sakaeratica. Six isolates of the novel species were obtained from different bromeliads and regions in Brazil. Sequence analysis of the D1/D2 domains of the large subunit of the rRNA gene showed that the novel species differs from B. miyagiana and B. sakaeratica by 85 and 64 nt substitutions, respectively and by more than 75 nt substitutions in the ITS region. Phenotypically, Bullera vrieseae sp. nov. can be distinguished from both species based on the assimilation of meso-erythritol, which was negative for B. vrieseae sp. nov. but positive for the others, assimilation of d-glucosamine, which was positive for B. vrieseae sp. nov. but negative for B. miyagiana and of l-sorbose, which was negative for B. vrieseae sp. nov. but positive for B. sakaeratica. The novel species Bullera vrieseae sp. nov. is proposed to accommodate these isolates. The type strain of Bullera vrieseae sp. nov. is UFMG-CM-Y379T (BRO443T; ex-type CBS 13870T).
Programa de Pós-Graduação em Diversidade Biológica e Conservação nos Trópicos, Universidade Federal de Alagoas, Maceió - AL, Brazil.|Setor de Genética/ICBS, Universidade Federal de Alagoas, Maceió - AL, Brazil.
Melissa Fontes
MF
Landell
Departamento de Microbiologia, ICB, C.P. 486, Universidade Federal de Minas Gerais, Belo Horizonte - MG 31270-901, Brazil.
Luciana R
LR
Brandão
Departamento de Microbiologia, ICB, C.P. 486, Universidade Federal de Minas Gerais, Belo Horizonte - MG 31270-901, Brazil.
Silvana V B
SVB
Safar
Departamento de Química, Centro Federal de Educação Tecnológica de Minas Gerais, Belo Horizonte - MG 30421-169, Brazil.
Fatima C O
FCO
Gomes
Setor de Genética/ICBS, Universidade Federal de Alagoas, Maceió - AL, Brazil.
Ciro R
CR
Félix
Departamento de Microbiologia, ICB, C.P. 486, Universidade Federal de Minas Gerais, Belo Horizonte - MG 31270-901, Brazil.
Ana Raquel O
ARO
Santos
Setor de Genética/ICBS, Universidade Federal de Alagoas, Maceió - AL, Brazil.|Programa de Pós-Graduação em Diversidade Biológica e Conservação nos Trópicos, Universidade Federal de Alagoas, Maceió - AL, Brazil.
Danielle M
DM
Pagani
National Reference Laboratory for Tuberculosis, Centro de Referência Professor Hélio Fraga, Escola Nacional de Saúde Pública, Fiocruz - RJ, Brazil.
Jesus P
JP
Ramos
Setor de Genética/ICBS, Universidade Federal de Alagoas, Maceió - AL, Brazil.
Leonardo
L
Broetto
Programa de Pós-Graduação em Diversidade Biológica e Conservação nos Trópicos, Universidade Federal de Alagoas, Maceió - AL, Brazil.
Tamí
T
Mott
Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre - RS, Brazil.
Marilene H
MH
Vainstein
Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal do Rio Grande do Sul, Porto Alegre - RS, Brazil.
Patricia
P
Valente
Departamento de Microbiologia, ICB, C.P. 486, Universidade Federal de Minas Gerais, Belo Horizonte - MG 31270-901, Brazil.
Carlos A
CA
Rosa
D001487:Basidiomycota / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001938:Brazil; D031229:Bromeliaceae / Q000382:microbiology*; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010802:Phylogeny; D017422:Sequence Analysis, DNA
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer