[Relationship between HSP70 gene polymorphisms and susceptibility to lung function injury of cock-oven workers].
OBJECTIVE
In this study, we investigate the relationship between HSP70 and lung function injury. To study on the feasibility of HSP70 genes polymorphisms as biological marker of the damage of pulmonary dysfunction susceptibility.


METHODS
183 cock-oven workers were selected as exposure groups and 143 workers unexposed workers were selected as control groups. We investigated their general information with uniform questionnaire. Pulmonary dysfunction indicators were determined using portable spirometer. HSP70-1 G190C, HSP70-2 A1267G, HSP70- hom T2437C genotypes were analyzed by using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. The haplotypes were calculated using PHASE 2.0 software.


RESULTS
VC%, FVC%, MVV%, FEV(1.0%) in exposed group were lower than in non-exposure group, the difference were significantly (P < 0.05). VC%, FVC%, MVV%, FEV1.0% in exposed group with HSP70-1, HSP70-2, HSP70-hom genotypes were lower than in non-exposure group (P < 0.05); FVC% in exposed group with HSP70-hom T/C genotypes were lower than that with HSP70-hom T/T genotypes, MVV% were lower than that with HSP70-hom T/T, C/C genotypes. There's no difference in pulmonary dysfunction index of HSP70-1, HSP70-2 genotypes (P>0.05), but significant difference between the exposed group with HSP70-1, HSP70-hom genotypes; The adjust OR (95%CI) of exposed group with HSP70-1 G/C genotypes and HSP70-homT/C genotypes were 2.516 (1.012 ∼6.252) and 2.284 (1.033∼5.053). Exposed group with CGT haplotype pulmonary dysfunction were significantly higher than in non-exposure group (P < 0.05).


CONCLUSIONS
Coke oven exposure may increase pulmonary dysfunction injury, Coke oven workers who have the HSP70-1 G/C genotypes, HSP70-hom T/C genotypes and CGT haplotypes may increase the susceptibility of pulmonary dysfunction. There must be some relationship between HSP70-1, HSP70-hom gene polymorphisms and lung function injury of Cock-oven Workers.
Department of Toxicology, Shanxi Medical University Taiyuan, Shanxi 030001, China.
Zhipeng
Z
Hu
Hongming
H
Zhang
Weixing
W
Li
Huifang
H
Zhang
Shanshan
S
Chang
Baoping
B
Wang
Zhiyu
Z
Li
E-mail: sytoxi6322@126.com.
Jinping
J
Zheng
D003077:Coke; D004198:Disease Susceptibility; D005838:Genotype; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D008168:Lung / Q000503:physiopathology; D016273:Occupational Exposure / Q000009:adverse effects*; D011110:Polymorphism, Genetic; D011795:Surveys and Questionnaires
D003077:Coke; D018840:HSP70 Heat-Shock Proteins; C081884:heat-shock protein 70.1