EGFR negates the proliferative effect of oncogenic HER2 in MDA-MB-231 cells.
Members of the EGFR family are potent mediators of normal cell growth and development. HER2 possesses an active tyrosine kinase domain, but no direct ligand has been identified. To investigate the differential effect of HER2 in breast cell lines, HER2 was overexpressed in MCF-10A, MCF7 and MDA-MB-231 cells. HER2 overexpression promoted proliferation, survival and migration in MCF-10A and MCF-7 cells. No significant differences were seen in proliferation, survival or migration between MDA-MB-231 vec and HER2 cells. The activity of downstream HER2 proteins increased in MCF-10A HER2 and MCF-7 HER2 cells but not in MDA-MB-231 HER2 cells. Exogenously expressed HER2 failed to associate with EGFR or HER3 in MDA-MB-231 cells, while overexpression of HER2 enhanced HER family dimerization in MCF-10A and MCF-7 cells.
Department of Life Science, Hanyang University, Seoul 133-791, Republic of Korea.
Sunhwa
S
Oh
Department of Life Science, Hanyang University, Seoul 133-791, Republic of Korea.
Ji-hyun
JH
Ju
Department of Life Science, Hanyang University, Seoul 133-791, Republic of Korea.
Wonseok
W
Yang
Department of Life Science, Hanyang University, Seoul 133-791, Republic of Korea.
Kyung-min
KM
Lee
Department of Life Science, Hanyang University, Seoul 133-791, Republic of Korea.
KeeSoo
K
Nam
Department of Life Science, Hanyang University, Seoul 133-791, Republic of Korea; Natural Science Institute, Hanyang University, Seoul 133-791, Republic of Korea. Electronic address: incheol@hanyang.ac.kr.
Incheol
I
Shin
D001483:Base Sequence; D001943:Breast Neoplasms / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D017931:DNA Primers; D066246:ErbB Receptors / Q000502:physiology*; D005260:Female; D018734:Genes, erbB-2; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D066246:ErbB Receptors
Breast cancer cell; EGFR; HER2; Triple negative breast cancer