Renal biomarkers of male and female Wistar rats (Rattus norvegicus) undergoing renal ischemia and reperfusion.
OBJECTIVE
To investigate biomarkers of acute renal injury in Wistar rats, subjected to left renal ischemia for 10 minutes, and then compare reperfusion at 24 hours, and at 5, 7, 14 and 21 days after the procedure.


METHODS
Eight female and male rats between 60 and 81 days old were used in the Central Animal Facility of the UFMS. Assessed biomarkers included urine protein, urea, creatinine, glucose, sodium, potassium, urine alkaline phosphatase and gamma-glutamyl transferase activities, and protein-to-creatinine ratio; and in serum: urea, creatinine, sodium and potassium, fractional excretion of sodium, potassium, urine flow and creatinine clearance.


RESULTS
Greater variance was observed in the parameters at 24 hours and at five days (p<0.05) after reperfusion. On the 21st day, these parameters approximated those obtained for the control group.


CONCLUSIONS
Renal ischemia for 10 minutes was sufficient to raise urine levels of protein, glucose, fractional excretion of potassium, urea, creatinine clearance, urine activity of gamma-glutamyltransferase and alkaline phosphatase enzymes in the first 24 hours, up to five days after reperfusion, which may indicate risk of acute kidney injury, according to the RIFLE classification.
UFMS, Campo Grande, MS, Brazil.
Telma
T
Bazzano
Tamy Ingrid
TI
Restel
UFES, Vitoria, ES, Brazil.
Lenir Cardoso
LC
Porfirio
UFMS, Campo Grande, MS, Brazil.
Albert Schiaveto de
AS
Souza
UFMS, Campo Grande, MS, Brazil.
Iandara Schettert
IS
Silva
D058186:Acute Kidney Injury / Q000097:blood / Q000652:urine*; D000469:Alkaline Phosphatase / Q000652:urine; D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000652:urine*; D003404:Creatinine / Q000097:blood / Q000652:urine; D005260:Female; D006029:Glycosuria; D007511:Ischemia / Q000097:blood / Q000652:urine*; D007668:Kidney / Q000098:blood supply*; D008297:Male; D011188:Potassium / Q000097:blood / Q000652:urine; D017208:Rats, Wistar; D012016:Reference Values; D015427:Reperfusion Injury / Q000097:blood / Q000652:urine*; D012307:Risk Factors; D012737:Sex Factors; D012964:Sodium / Q000097:blood / Q000652:urine; D013997:Time Factors; D014508:Urea / Q000097:blood / Q000652:urine; D005723:gamma-Glutamyltransferase / Q000652:urine
D015415:Biomarkers; D014508:Urea; D012964:Sodium; D003404:Creatinine; D005723:gamma-Glutamyltransferase; D000469:Alkaline Phosphatase; D011188:Potassium