Evaluation of Gastrothylax crumenifer antigenic preparation in serodiagnosis of paramphistomiasis in sheep.
An evaluation of Gastrothylax crumenifer crude antigen preparation viz., Somatic Antigen (SAg), Excretory Secretory Antigen (ESAg) and Egg Antigen (EAg) in serodiagnosis of disease was undertaken. Test sera samples were obtained from 30 Paramphistomiasis Positive and 30 Gastrothylax free sheep slaughtered at Hazratbal Kashmir. The referral antigenic preparation were evaluated against Paramphistomiasis positive sera, via., control negative sera, using double immunodiffusion test (DID), (IEP) Immunoelectrophoretic assay and ELISA. The performance of referral antigens, as assessed from percent sensitivity and specificity, revealed an increasing trend from DID (Double immunodiffusion-An immunological technique used in the detection, identification and quantification of antibodies and antigens) to IEP (immunoelectrophoresis-A general name for a number of biochemical methods for separation and characterization of proteins based on electrophoresis and reaction with antibodies), followed by ELISA, detecting higher number of sheep positive for paramphistomiasis. In ELISA the ESAg and SAg were evaluated as most reactive antigens with no significant difference and EAg was the least antigenic. In IEP, EAg had the higher sensitivity (60%) and analogous specificity of SAg and ESAg. The formation of the preceptin lines in the proximity to EAg containing wells (cathode end) in IEP was suggestive of higher molecular weight of G. crumenifer specific protein molecules with slower rate of migration. Purification and characterization of G. crumenifer and identification of specific antigenic molecules, particularly in EAg has been suggested for qualitative improvement of diagnostic value of the antigens in the tests used here in.
Tariq
T
Ahmad
Mohammad Latif
ML
Reshi
M Z
MZ
Cheshti
Syed
S
Tanveer
Zaffar Amin
ZA
Shah
Bashir Ahmad
BA
Fomada
O K
OK
Raina
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005779:Immunodiffusion / Q000662:veterinary; D007122:Immunoelectrophoresis / Q000662:veterinary; D010250:Paramphistomatidae / Q000276:immunology*; D011237:Predictive Value of Tests; D012698:Serologic Tests / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000097:blood / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology; D034561:Sheep, Domestic; D014201:Trematode Infections / Q000097:blood / Q000175:diagnosis / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*
D000947:Antigens, Helminth