Morphometric and stereological assessment of the effects of zinc oxide nanoparticles on the mouse testicular tissue.
OBJECTIVE
To evaluate the effects of zinc oxide nanoparticles (ZNPs) on morphometric and stereological parameters of mouse testis.


BACKGROUND
ZNPs are increasingly used in sunscreens, biosensors, food additives, pigments, rubber manufacture, and electronic materials. However, the potential toxicity of these nanoparticles is not well understood.


METHODS
Experimental Groups (ZNP-1, ZNP-2 and ZNP-3) received one of the following treatments daily for 35 days: 5, 50 and 300 mg/kg ZNPs respectively. Right testis from each animal was fixed in bouin's solution for measurement of total volume of testis, total volume of seminiferous tubules, total volume of interstitial tissue and total number of Leydig cells by stereological methods. Seminiferous tubule diameter and seminiferous epithelium height were assessed by morphometrical method. The left testicles were homogenized for measurement of testosterone concentration.


RESULTS
There was a significant decrease in the weight of testicles in ZNP-3 groups. The stereological and morphometrical parameters were significantly changed in ZNP-2 and ZNP-3 groups. ZNP-2 and ZNP-3 groups also showed a significant decrease in testosterone concentrations (p < 0.05).


CONCLUSIONS
This study demonstrated that ZNPs can change stereological and morphometrical parameters of the seminiferous tubules and reduce the number of Leydig cells (Tab. 3, Fig. 3, Ref. 36).
M
M
Moridian
L
L
Khorsandi
A R
AR
Talebi
D000818:Animals; D001835:Body Weight; D007985:Leydig Cells / Q000187:drug effects*; D008297:Male; D051379:Mice; D053758:Nanoparticles; D009929:Organ Size; D012671:Seminiferous Tubules / Q000187:drug effects*; D013473:Sunscreening Agents / Q000494:pharmacology*; D013737:Testis / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D013739:Testosterone / Q000378:metabolism*; D015034:Zinc Oxide / Q000494:pharmacology*
D013473:Sunscreening Agents; D013739:Testosterone; D015034:Zinc Oxide
Leydig cells; seminiferous tubules; stereology.; zinc oxide nanoparticles