Immunomodulation of intestinal macrophages by mercury involves oxidative damage and rise of pro-inflammatory cytokine release in the fresh water fish Channa punctatus Bloch.
Mercury and its compounds have been parts of widespread pollutants of the aquatic environment. The present study was designed to assess the effect of mercury on fish immune responses. Since the metal is absorbed by fish and passed up the food chain to other fish-eating species, it not only affects aquatic ecosystems but also humans through bioaccumulation. In the present study, it was found that innate immunity of the fresh water fish Channa punctatus Bloch. was significantly debilitated after a periods of exposure to a sub-lethal concentration of mercury (0.3 mg/L). After 7 days of exposure, phagocytosis, cell adhesion and intracellular killing activity were found to decrease significantly along with significant decreases in nitric oxide (NO) and myeloperoxidase (MPO) production from macrophages as compared to the control group indicating intracellular damages. Levels of pro-inflammatory cytokines like TNF-α and IL-6 were found to be significantly more in mercury treated groups than that of control group indicating inflammatory damage. This included significant ultrastructural changes like fragmented epithelium, lesions in mucosal foldings, degenerated mitochondria, reduction in the number of goblet cells and disoriented microvilli as evident from transmission electron micrographs.
Immunobiology Laboratory, Department of Biotechnology, Assam University, Silchar, Assam 788011, India.
Moriom
M
Begam
Immunobiology Laboratory, Department of Biotechnology, Assam University, Silchar, Assam 788011, India. Electronic address: mahuya.sengupta74@gmail.com.
Mahuya
M
Sengupta
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D016207:Cytokines / Q000276:immunology; D005399:Fishes; D056747:Immunomodulation; D015850:Interleukin-6 / Q000276:immunology; D007422:Intestines / Q000187:drug effects* / Q000276:immunology / Q000473:pathology / Q000648:ultrastructure; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D008628:Mercury / Q000633:toxicity*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D009569:Nitric Oxide / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D016207:Cytokines; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D014874:Water Pollutants, Chemical; D009569:Nitric Oxide; D009195:Peroxidase; D008628:Mercury
Channa punctatus; Intracellular killing; Mercury exposure; Myeloperoxidase; Nitric oxide; Phagocytic index