Sensitive, fast, and specific immunoassays for methyltestosterone detection.
An indirect competitive enzyme-linked immunosorbent assay (icELISA) and an immunochromatographic strip assay using a highly specific monoclonal antibody, were developed to detect methyltestosterone (MT) residues in animal feed. The optimized icELISA had a half-inhibition concentration value of 0.26 ng/mL and a limit of detection value of 0.045 ng/mL. There was no cross-reactivity with eight analogues, revealing high specificity for MT. Based on icELISA results, the recovery rate of MT in animal feed was 82.4%-100.6%. The results were in accordance with those obtained by gas chromatography-mass spectrometry. The developed immunochromatographic strip assay, as the first report for MT detection, had a visual cut-off value of 1 ng/mL in PBS, 2.5 ng/g in fish feed, and 2.5 ng/g in pig feed. Therefore, these immunoassays are useful and fast tools for MT residue detection in animal feed.
State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. kongxiyangsucc@126.com.
Na
N
Kong
State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. songshanshan0626@126.com.
Shanshan
S
Song
State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. pengjuan2016@163.com.
Juan
J
Peng
State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. raxray@gmail.com.
Liqiang
L
Liu
State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. kuangh@jiangnan.edu.cn.
Hua
H
Kuang
State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. xcl@jiangnan.edu.cn.
Chuanlai
C
Xu
D000821:Animal Feed / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry; D007118:Immunoassay / Q000379:methods*; D008777:Methyltestosterone / Q000032:analysis*; D015203:Reproducibility of Results
D008777:Methyltestosterone