Expression and characterization of a lytic polysaccharide monooxygenase from Bacillus thuringiensis.
Lytic polysaccharide monooxygenases (LPMOs) are recently discovered oxidative enzymes that are capable of oxidative cleavage of recalcitrant polysaccharides such as chitin or cellulose. Despite the importance of LPMOs in biomass conversion and the large number of lpmo genes in microorganisms, only a few LPMOs have been well studied, and further characterization of these proteins is thus of interest. In this study, a chitin-active AA10 family LPMO from Bacillus thuringiensis subsp. kurstaki, BtLPMO10A, is described. This enzyme generates even-numbered oxidized oligosaccharides as the dominated products from crystalline chitin, however, interestingly, when colloidal chitin is used as the substrate, a ladder of oxidized oligosaccharides is observed. These results provide new insights into the action mode of LPMOs that may be affected by the substrates.
School of Food Science and Technology, Dalian Polytechnic University, Dalian, 116034, China; Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.
Huiyan
H
Zhang
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.
Yong
Y
Zhao
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.
Hailong
H
Cao
School of Food Science and Technology, Dalian Polytechnic University, Dalian, 116034, China. Electronic address: gq6552002@aliyun.com.
Guangqing
G
Mou
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China. Electronic address: yinheng@dicp.ac.cn.
Heng
H
Yin
D001413:Bacillus thuringiensis / Q000737:chemistry* / Q000201:enzymology; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D020134:Catalytic Domain; D002686:Chitin / Q000737:chemistry*; D003001:Cloning, Molecular; D003102:Colloids; D003460:Crystallization; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006868:Hydrolysis; D006899:Mixed Function Oxygenases / Q000737:chemistry* / Q000235:genetics; D009844:Oligosaccharides / Q000737:chemistry*; D010084:Oxidation-Reduction; D011485:Protein Binding; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D013379:Substrate Specificity
D001426:Bacterial Proteins; D003102:Colloids; D009844:Oligosaccharides; D011994:Recombinant Proteins; D002686:Chitin; D006899:Mixed Function Oxygenases
B. thuringiensis; Lytic polysaccharide monooxygenases; Polysaccharides