Breeding of 'Manten-Kirari', a non-bitter and trace-rutinosidase variety of Tartary buckwheat (Fagopyrum tataricum Gaertn.).
Here, we developed a new Tartary buckwheat cultivar 'Manten-Kirari', whose flour contains only trace amounts of rutinosidase and lacked bitterness. The trace-rutinosidase breeding line 'f3g-162' (seed parent), which was obtained from a Nepalese genetic resource, was crossed with 'Hokkai T8' (pollen parent), the leading variety in Japan, to improve its agronomic characteristics. The obtained progeny were subjected to performance test. 'Manten-Kirari' had no detectable rutinosidase isozymes in an in-gel detection assay and only 1/266 of the rutinosidase activity of 'Hokkai T8'. Dough prepared from 'Manten-Kirari' flour contained almost no hydrolyzed rutin, even 6 h after the addition of water, whereas the rutin in 'Hokkai T8' dough was completely hydrolyzed within 10 min. In a sensory evaluation of the flour from the two varieties, nearly all panelists detected strong bitterness in 'Hokkai T8', whereas no panelists reported bitterness in 'Manten-Kirari'. This is the first report to describe the breeding of a Tartary buckwheat cultivar with reduced rutin hydrolysis and no bitterness in the prepared flour. Notably, the agronomic characteristics of 'Manten-Kirari' were similar to those of 'Hokkai T8', which is the leading variety in Japan. Based on these characteristics, 'Manten-Kirari' is a promising for preparing non-bitter, rutin-rich foods.
NARO Hokkaido Agricultural Research Center , Hitsujigaoka, Toyohira, Sapporo, Hokkaido 062-8555 , Japan.
Tatsuro
T
Suzuki
NARO Hokkaido Agricultural Research Center, Memuro Upland Farming Research Station , Shinsei, Memuro, Kasai, Hokkaido 082-0081 , Japan.
Toshikazu
T
Morishita
NARO Hokkaido Agricultural Research Center, Memuro Upland Farming Research Station , Shinsei, Memuro, Kasai, Hokkaido 082-0081 , Japan.
Yuji
Y
Mukasa
NARO Hokkaido Agricultural Research Center, Memuro Upland Farming Research Station , Shinsei, Memuro, Kasai, Hokkaido 082-0081 , Japan.
Shigenobu
S
Takigawa
NARO Hokkaido Agricultural Research Center, Memuro Upland Farming Research Station , Shinsei, Memuro, Kasai, Hokkaido 082-0081 , Japan.
Satoshi
S
Yokota
NARO Hokkaido Agricultural Research Center, Memuro Upland Farming Research Station , Shinsei, Memuro, Kasai, Hokkaido 082-0081 , Japan.
Koji
K
Ishiguro
NARO Hokkaido Agricultural Research Center, Memuro Upland Farming Research Station , Shinsei, Memuro, Kasai, Hokkaido 082-0081 , Japan.
Takahiro
T
Noda
Tartary buckwheat; bitterness; breeding; cross breeding; genetic resources; quality; rutinosidase