Role of Hsp-70 responses in cold acclimation of HUVEC-12 cells.
BACKGROUND
Endothelial recovery is a central feature of tissues after frostbite injuries. Thermo tolerance plays an important role in protecting cells against injury after frozen and thawing. The present study aimed to quantitatively assess the injury of human umbilical vein endothelial cells HUVEC-12 after repeated low temperature.


METHODS
Pretreatments (HUVEC-12) cells were repeatedly exposed to cold (1°C/min decrement to -20°C). Their proliferation, death, apoptosis, and protein and mRNA expressions of HSP70 were determined.


RESULTS
Endothelial cells after repeated cold exposures were more resistant to apoptosis and necrosis than normal cells. The expressions of HSP70 in cells after repeated cold exposures were significantly higher than in normal HUVEC-12 cells (P < 0.05).


CONCLUSIONS
Cold acclimation may induce the expression of HSP-70 which plays a protective role in the temperature tolerance.
Department of Burn, Xijing Hospital, The Fourth Military Medical University Xi'an 710032, Shanxi, China.
Hao
H
Guan
Department of Burn, Xijing Hospital, The Fourth Military Medical University Xi'an 710032, Shanxi, China.
Dahai
D
Hu
Department of Cardiology, Xijing Hospital, The Fourth Military Medical University Xi'an 710032, Shanxi, China.
Zhijing
Z
Zhao
Department of Burn, Xijing Hospital, The Fourth Military Medical University Xi'an 710032, Shanxi, China.
Weixia
W
Cai
Department of Burn, Xijing Hospital, The Fourth Military Medical University Xi'an 710032, Shanxi, China.
Qin
Q
Zhou
Department of Burn, Yan'an University Affiliated Hospital, Yanan University Yan'an 710032, Shanxi, China.
Ximing
X
Yang
Department of Burn, 413 Hospital of People's Liberation Army Zhoushan 316000, Zhejiang, China.
Ying
Y
Yan
Department of Burn, Xijing Hospital, The Fourth Military Medical University Xi'an 710032, Shanxi, China.
Xiongxiang
X
Zhu
Temperature; heat shock protein 70; human umbilical vein endothelial cells-12; thawing