Escherichia coli BL21(DE3) chromosome-based controlled intracellular processing system for fusion protein separation.
A chromosome-based controlled intracellular processing system was constructed by integrating the TEV protease gene into the chromosome of E. coli BL21(DE3) with λ-Red recombineering. The system is highly efficient in processing maltose binding protein fusion protein; the separated protein could be purified to near homogeneity with Ni-NTA affinity chromatography.
Jiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Sciences, Nanjing Normal University, Jiangsu Province, PR China.
Xi
X
Luo
Jiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Sciences, Nanjing Normal University, Jiangsu Province, PR China.
Ling
L
Li
Jiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Sciences, Nanjing Normal University, Jiangsu Province, PR China.
Meixia
M
Chai
Jiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Sciences, Nanjing Normal University, Jiangsu Province, PR China.
Qing
Q
Zhang
Jiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Sciences, Nanjing Normal University, Jiangsu Province, PR China. Electronic address: shanggd@hotmail.com.
Guangdong
G
Shang
D002876:Chromosomes, Bacterial; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D060847:Metabolic Engineering / Q000379:methods*; D011499:Protein Processing, Post-Translational; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D011995:Recombination, Genetic
D011993:Recombinant Fusion Proteins; D010450:Endopeptidases; C099488:TEV protease
Controlled intracellular processing; Escherichia coli BL21(DE3); In vivo digestion; Recombineering; TEV protease