Acute phase proteins altered in the plasma of patients with congenital ventricular septal defect.
OBJECTIVE
Ventricular septal defect (VSD) has intracardiac left-to-right shunt and increased pulmonary flow that may affect the acute phase response (APR). We examined the hypothesis that plasma proteins of VSD patients may be altered.


METHODS
2DE and MS were used to detect differential plasma proteins in VSD patients (n = 55) and controls (n = 70). Candidate APR proteins were confirmed by ELISA in new samples.


RESULTS
Among three differentially expressed APR proteins from 322 protein spots detected, haptoglobin (0.4 ± 0.04 versus 0.6 ± 0.07 mg/mL; p = 0.016) and serum amyloid P-component (SAP) (3.8 ± 0.2 versus 6.3 ± 0.8 ng/mL; p = 0.003) were significantly lower and orosomucoid 2 (3.1 ± 0.1 mg/mL versus 2.3 ± 0.1 mg/mL; p < 0.001) was significantly higher in VSD patients than in normal controls.


CONCLUSIONS
The plasma concentration of three acute phase proteins, haptoglobin, SAP, and orosomucoid 2 are altered that may reflect inflammation, be associated with decreased innate immune system function, and predispose the VSD patients to vulnerability to infections and pulmonary disease. These three proteins in plasma may also be developed as biomarkers for the function of innate immune system in patients with congenital heart disease.
TEDA International Cardiovascular Hospital, Tianjin and The Affiliated Hospital of Hangzhou Normal University and Zhejiang University, Hangzhou, China.
Xi
X
Zhang
Department of Cardiovascular Surgery, Tianjin First Center Hospital, Tianjin, China.
Kai
K
Wang
TEDA International Cardiovascular Hospital, Tianjin and The Affiliated Hospital of Hangzhou Normal University and Zhejiang University, Hangzhou, China.|Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Hong Kong, China.
Qin
Q
Yang
TEDA International Cardiovascular Hospital, Tianjin and The Affiliated Hospital of Hangzhou Normal University and Zhejiang University, Hangzhou, China.
Jun
J
Wang
TEDA International Cardiovascular Hospital, Tianjin and The Affiliated Hospital of Hangzhou Normal University and Zhejiang University, Hangzhou, China.
Chao
C
Xuan
TEDA International Cardiovascular Hospital, Tianjin and The Affiliated Hospital of Hangzhou Normal University and Zhejiang University, Hangzhou, China.
Xiao-Cheng
XC
Liu
TEDA International Cardiovascular Hospital, Tianjin and The Affiliated Hospital of Hangzhou Normal University and Zhejiang University, Hangzhou, China.
Zhi-Gang
ZG
Liu
TEDA International Cardiovascular Hospital, Tianjin and The Affiliated Hospital of Hangzhou Normal University and Zhejiang University, Hangzhou, China.|Department of Surgery, Oregon Health and Science University, Portland, Oregon, USA.
Guo-Wei
GW
He
D000209:Acute-Phase Proteins / Q000378:metabolism*; D002675:Child, Preschool; D005260:Female; D006345:Heart Septal Defects, Ventricular / Q000097:blood*; D006801:Humans; D008297:Male; D040901:Proteomics; D015203:Reproducibility of Results
D000209:Acute-Phase Proteins
Cardiac surgery; Congenital heart disease; Ventricular septal defect