An amplified electrochemical strategy using DNA-QDs dendrimer superstructure for the detection of thymine DNA glycosylase activity.
A triple-signal amplification strategy was proposed for highly sensitive and selective detection of thymine DNA glycosylase (TDG) by coupling a dendrimer-like DNA label with the electrochemical method and quantum dots (QDs) tagging. The DNA-QDs dendrimer-like superstructure was designed by DNA hybridization and covalent assembling. Benefiting from outstanding performance of the amplification strategy, this assay showed high sensitivity, extraordinary stability, and easy operation. The limit of detection could reach 0.00003 U µL(-1) with a splendid specificity. The TDG content in different concentration of HeLa cell was also determined. This assay opens a new horizon for both qualitative and quantitative detection of TDG, holding great promise for potential application in cancer cell research and clinical diagnostics.
State Key Lab of Analytical Chemistry for Life Science, School of Chemistry & Chemical Engineering, Nanjing University, Nanjing 210093, China; College of Life Information Science & Instrument Engineering, Hangzhou Dianzi University, Hangzhou 310018, China.
Hongying
H
Liu
School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, PR China.
Youbing
Y
Lou
State Key Lab of Analytical Chemistry for Life Science, School of Chemistry & Chemical Engineering, Nanjing University, Nanjing 210093, China.
Fei
F
Zhou
State Key Lab of Analytical Chemistry for Life Science, School of Chemistry & Chemical Engineering, Nanjing University, Nanjing 210093, China.
Hao
H
Zhu
Petrochemical Research Chair, Chemistry Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
E S
ES
Abdel-Halim
State Key Lab of Analytical Chemistry for Life Science, School of Chemistry & Chemical Engineering, Nanjing University, Nanjing 210093, China. Electronic address: jjzhu@nju.edu.cn.
Jun-Jie
JJ
Zhu
D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D050091:Dendrimers / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods; D057075:Enzyme Assays / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D057230:Limit of Detection; D008958:Models, Molecular; D045663:Quantum Dots / Q000737:chemistry*; D015203:Reproducibility of Results; D044502:Thymine DNA Glycosylase / Q000032:analysis / Q000378:metabolism*
D050091:Dendrimers; D004247:DNA; D044502:Thymine DNA Glycosylase
Dendrimer superstructure; Electrochemical biosensor; Quantum dots; Signal amplification; Thymine DNA glycosylase