[Molecular identification in genus of Lilium based on DNA barcoding].
To establish a new method for identifying genus of Lilium by DNA barcoding technology, ITS, ITS2, psbA-trnH, matK and rbcL sequences were analyzed in term of variation of inter- and intra-species, barcoding gap, neighbor-joining tree to distinguish genus of Lilium based on 978 sequences from experimental and GenBank database, and identification efficiency was evaluated by Nearest distance and BLAST1 methods. The results showed that DNA barcoding could identify different species in genus of Lilium. ITS sequence performed higher identification efficiency, and had significant difference between intra- and inter-species. And NJ tree could also divide species into different clades. Results indicate that DNA barcoding can identify genus of Lilium accurately. ITS sequence can be the optimal barcode to identify species of Lilium.
Si-Hao
SH
Zheng
Ya-Kang
YK
Li
Wei-Guang
WG
Ren
Lin-Fang
LF
Huang
D058893:DNA Barcoding, Taxonomic; D018744:DNA, Plant / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D027762:Lilium / Q000145:classification*
D018744:DNA, Plant; D021903:DNA, Ribosomal Spacer