A thermostable archaeal S-adenosylmethionine synthetase: a promising tool to improve the synthesis of adenosylmethionine analogs of biotechnological interest.
The naturally and widely occurring sulfonium compound, S-adenosylmethionine (AdoMet), one of nature's most versatile molecules, is biosynthesized from methionine and ATP by AdoMet synthetase or methionine adenosyltransferase (MAT) in a 2-step reaction in which the energy-rich sulfonium compound is formed by dephosphorylation of ATP. All living cells, with the only exception of some parasites and infectious agents, express MAT.
a Dipartimento di Biochimica; Biofisica e Patologia Generale ; Seconda Università di Napoli ; Napoli , Italy.
Marina
M
Porcelli
Concetta Paola
CP
Ilisso
Laura
L
Mosca
Giovanna
G
Cacciapuoti
D013766:5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase / Q000378:metabolism; D025181:ATP Synthetase Complexes / Q000378:metabolism; D001105:Archaea / Q000201:enzymology*; D055598:Chemical Phenomena; D007218:Industrial Microbiology; D008716:Methionine Adenosyltransferase / Q000378:metabolism*; D012436:S-Adenosylmethionine / Q000031:analogs & derivatives* / Q000096:biosynthesis*
D012436:S-Adenosylmethionine; D013766:5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase; D008716:Methionine Adenosyltransferase; D025181:ATP Synthetase Complexes
AdoMet, S-Adenosylmethionine; Archaea; BsMAT, MAT from Bacillus subtilis; DMSO, dimethylsulfoxide; MAT, adenosylmethionine synthetase or methionine adenosyltransferase; EcMAT, MAT from E. coli.; MjMAT, MAT from Methanococcus jannaschii; PfMAT, MAT from Pyrococcus furiosus; Pyrococcus furiosus; S-Adenosylmethionine; SsMAT, MAT from Sulfolobus solfataricus; TkMAT, MAT from Thermococcus kodakarensis; adenosylmethionine synthetase; hyperthermophilic enzymes; methionine adenosyltransferase; organic solvent activity and thermostability