[Chemical constituents of Taxus chinensis var. mairei cell cultures].
The chemical constituents of Taxus chinensis var. mairei cell cultures were investigated by chromatographic methods, including silica gel column chromatography, Sephadex LH-20 and preparative HPLC. Thirteen compounds were isolated from the 80% ethanol extract of cultured cells and their structures were elucidated by spectral data and physicochemical properties, which were identified as 2α,4α,7β,9α,10β-pentaacetoxy-14β-hydroxytax-11-ene (1), 2α,4α,7β,9α,10β-pentaacetoxytax-11-ene (2), 1β-deoxybaccatin VI (3), 2α-acetoxytaxusin (4), taxuyunnanine C (5), yunnanxane (6), 2α,5α,10β-triacetoxy-14β-propionyloxy-4 (20), 11-taxadiene (7), 2α,5α,10β-triacetoxy-14β-isobutyryloxy-4 (20), 11-taxadiene (8), 2α,5α,10β-triacetoxy-14β-(2'-methyl)butyryloxy-4 (20), 11-taxadiene (9), 13-dehydroxylbaccatin III (10), 13-dehydroxy-10-deacetylbaccatin III (11), paclitaxel (12) and (13) β-sitosterol. Among them, compound 1 is a new compound, and compounds 2, 4, 10 and 11 are isolated from the cell culture of Taxus chinensis var. mairei for the first time.
Xiang-yang
XY
Bai
Jian-ming
JM
Lü
Yan-ying
YY
Zhou
Zi-rong
ZR
Zhu
Ren-wang
RW
Jiang
Wei
W
Zhang
D000475:Alkenes / Q000032:analysis; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D004224:Diterpenes / Q000032:analysis; D015394:Molecular Structure; D017239:Paclitaxel / Q000032:analysis; D012855:Sitosterols / Q000032:analysis; D043823:Taxoids / Q000032:analysis; D020946:Taxus / Q000737:chemistry*
D000475:Alkenes; D004224:Diterpenes; D012855:Sitosterols; D043823:Taxoids; C093125:taxa-4(5),11(12)diene; C494600:taxuyunnanine C; C076298:yunnanxane; C042213:10-deacetylbaccatine III; C025473:gamma-sitosterol; D017239:Paclitaxel