Downregulation of plasma miR-451 and miR-16 in Plasmodium vivax infection.
Malaria is a common parasitic disease in tropical countries, causing one to two million deaths every year. To establish the new biomarker, we analyzed plasma miRNAs obtained from 19 malaria patients and 19 normal subjects, using reverse transcription-based quantitative polymerase chain reaction (RT-qPCR). The average levels of plasma miR-451 and miR-16 were significantly lower in malaria patients, (8.9-fold; p <0.001 and 10.4-fold; p = 0.01, respectively). The levels of other abundant miRNAs in plasma (miR-223, miR-226-3p) did not change significantly in malaria patients. Our data suggest that plasma miR-451 and miR-16 are relevant biomarkers for malaria infection.
Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand.
Supat
S
Chamnanchanunt
Department of Medical Technology, Graduate School of Health Sciences, Kyushu University, Fukuoka, Japan.
Chieri
C
Kuroki
Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand.
Varunee
V
Desakorn
Department of Medical Technology, Graduate School of Health Sciences, Kyushu University, Fukuoka, Japan.
Mari
M
Enomoto
Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand.
Vipa
V
Thanachartwet
Information Technology Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand.
Duangjai
D
Sahassananda
Mahidol Vivax Research Unit, Faculty of Tropical Medicine, Mahidol University, Rachathevi, Bangkok, 10400, Thailand.
Jetsumon
J
Sattabongkot
Mahidol Vivax Research Unit, Faculty of Tropical Medicine, Mahidol University, Rachathevi, Bangkok, 10400, Thailand.
Rachaneeporn
R
Jenwithisuk
Thalassemia Research Centre, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom 73170, Thailand.
Suthat
S
Fucharoen
Thalassemia Research Centre, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom 73170, Thailand.
Saovaros
S
Svasti
Department of Medical Technology, Graduate School of Health Sciences, Kyushu University, Fukuoka, Japan; Department of Medical Technology and Sciences, International University of Health and Welfare, Fukuoka, Japan. Electronic address: umemura@med.kyushu-u.ac.jp.
Tsukuru
T
Umemura
D000328:Adult; D017677:Age Distribution; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D015536:Down-Regulation; D004912:Erythrocytes / Q000469:parasitology; D006801:Humans; D016778:Malaria, Falciparum / Q000097:blood / Q000175:diagnosis / Q000235:genetics; D016780:Malaria, Vivax / Q000097:blood* / Q000175:diagnosis / Q000235:genetics; D035683:MicroRNAs / Q000097:blood*; D018512:Parasitemia / Q000097:blood; D012372:ROC Curve; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index; D055815:Young Adult
D015415:Biomarkers; C505362:MIRN16 microRNA, human; C531201:MIRN451 microRNA, human; D035683:MicroRNAs
Biomarker; Malaria; Plasma miRNAs; Plasmodium vivax; miR-16; miR-451