Time impact on non-activated and kaolin-activated blood samples in thromboelastography.
BACKGROUND
The correct methodology of thrombelastography might be influenced by elapsing time. In our study we investigated kaolin activated citrated samples together with non-activated citrated samples in relation to the elapsed times of 0, 15 and 30 minutes to compare both methods and to find out if there is an impact of time on results of thrombelastography.


METHODS
Blood samples obtained from 10 healthy volunteers were analyzed after 0, 15 and 30 minutes from sampling with kaolin activation and without activation. Then the results were analysed and compared between the non-activated and the kaolin-activated method.


RESULTS
All blood samples became more hypercoagulable with the time elapsing, both in non-activated and kaolin-activated samples and differences between both groups were found statistically and clinically significant after only 0 minutes.


CONCLUSIONS
The non-activated citrated method seems to be reliable and suitable for thrombelastography in non-emergency cases (planned surgical procedures) when we have time to wait 15-30 minutes to get results. In urgent situations a rapid thrombelastography test should be preferred. Although the kaolin-activated method can also be used, results must be interpreted with caution.
Department of Anaesthesiology and Intensive Care Medicine, Second Faculty of Medicine, Charles University in Prague, University Hospital Motol, V Úvalu 84, 150 06, Prague, 5, Czech Republic. durila4@gmail.com.
Miroslav
M
Durila
Department of Anaesthesiology and Intensive Care Medicine, Second Faculty of Medicine, Charles University in Prague, University Hospital Motol, V Úvalu 84, 150 06, Prague, 5, Czech Republic. pavellukas78@gmail.com.
Pavel
P
Lukáš
Department of Paediatrics, Charles University, Second Faculty of Medicine, Prague, Czech Republic. jiribronsky@gmail.com.
Jiří
J
Bronský
Department of Anaesthesiology and Intensive Care Medicine, Second Faculty of Medicine, Charles University in Prague, University Hospital Motol, V Úvalu 84, 150 06, Prague, 5, Czech Republic. karel.cvachovec@fnmotol.cz.
Karel
K
Cvachovec
D000704:Analysis of Variance; D001800:Blood Specimen Collection / Q000379:methods*; D002951:Citrates / Q000494:pharmacology; D003029:Coagulants / Q000494:pharmacology*; D064368:Healthy Volunteers; D006801:Humans; D007616:Kaolin / Q000494:pharmacology*; D013916:Thrombelastography / Q000379:methods*; D013997:Time Factors
D002951:Citrates; D003029:Coagulants; D007616:Kaolin