M-CSF in a new biomarker panel with HE4 and CA 125 in the diagnostics of epithelial ovarian cancer patients.
BACKGROUND
We investigated plasma levels of M-CSF and conventional tumor markers (HE4 and CA 125) in epithelial ovarian cancer patients as compared to control groups: benign ovarian tumor patients (cysts) and healthy subjects.


METHODS
M-CSF levels were determined by ELISA, HE4 and CA 125 levels - by CMIA method.


RESULTS
Our results have demonstrated significant differences in the concentration levels of M-CSF, CA 125 and HE4 between the groups of ovarian cancer patients, cysts patients and the healthy controls. In the groups tested M-CSF demonstrated equal to or higher values than both CA 125 and HE4 in diagnostic sensitivity (SE), positive and negative predictive values (PPV, NPV), and in the area under the ROC curve (AUC), particularly in the group with the serous epithelial sub-type of OC. Moreover, CA 125 showed better results of the aforementioned diagnostic criteria than HE4. The combined use of the parameters studied resulted in a further, significant increase in the value of the diagnostic indicators and in the value of the diagnostic power (AUC), especially in the early stages of ovarian cancer.


CONCLUSIONS
These findings suggest a high usefulness of M-CSF in diagnosing the serous sub-type of epithelial ovarian cancer and in discriminating between cancer and non-carcinoma lesions, particularly in new diagnostic panels in combination with CA 125 and HE4 for the detection of EOC in the early stages.
Department of Haematological Diagnostics, Medical University, Białystok, Poland. grazyna.bedkowska@umb.edu.pl.
Grażyna Ewa
GE
Będkowska
Department of Biochemical Diagnostics, Medical University Białystok, Waszyngtona 15A, Białystok, 15-269, Poland. slawicki@umb.edu.pl.
Sławomir
S
Ławicki
Department of Perinatology, Medical University, Białystok, Poland. sunnyeve@wp.pl.
Ewa
E
Gacuta
Department of Pediatric Ophthalmology with Squint Treatment Unit, Medical University, Białystok, Poland. przem38@wp.pl.
Przemysław
P
Pawłowski
Department of Biochemical Diagnostics, Medical University Białystok, Waszyngtona 15A, Białystok, 15-269, Poland. zdb@umwb.edu.pl.
Maciej
M
Szmitkowski
D000368:Aged; D000369:Aged, 80 and over; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000097:blood*; D018394:CA-125 Antigen / Q000097:blood; D000077216:Carcinoma, Ovarian Epithelial; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008163:Luminescent Measurements; D016173:Macrophage Colony-Stimulating Factor / Q000097:blood*; D008875:Middle Aged; D009375:Neoplasms, Glandular and Epithelial / Q000097:blood* / Q000175:diagnosis*; D010051:Ovarian Neoplasms / Q000097:blood* / Q000175:diagnosis*; D011506:Proteins / Q000032:analysis; D012372:ROC Curve; D012680:Sensitivity and Specificity; D000081102:WAP Four-Disulfide Core Domain Protein 2
D014408:Biomarkers, Tumor; D018394:CA-125 Antigen; D011506:Proteins; D000081102:WAP Four-Disulfide Core Domain Protein 2; C569698:WFDC2 protein, human; D016173:Macrophage Colony-Stimulating Factor