Enhanced aphid detoxification when confronted by a host with elevated ROS production.
Reactive oxygen species (ROS) plays an important role in plant defense responses against bacteria, fungi and insect pests. Most recently, we have demonstrated that loss of Arabidopsis thaliana BOTRYTIS-INDUCED KINASE1 (BIK1) function releases its suppression of aphid-induced H2O2 production and cell death, rendering the bik1 mutant more resistant to green peach aphid (Myzus persicae) than wild-type plants. However, little is known regarding how ROS-related gene expression is correlated with bik1-mediated resistance to aphids, or whether these aphids biochemically respond to the oxidative stress. Here, we show that the bik1 mutant exhibited elevated basal expression of ROS-generating and -responsive genes, but not ROS-metabolizing genes. Conversely, we detected enhanced detoxification enzymatic activities in aphids reared on bik1 plants compared to those on wild-type plants, suggesting that aphids counter the oxidative stress associated with bik1 through elevated metabolic resistance.
a Department of Entomology ; Institute for Plant Genomics & Biotechnology ; Texas A&M University; College Station , TX USA.
Jiaxin
J
Lei
Keyan
K
Zhu-Salzman
D000818:Animals; D001042:Aphids / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics / Q000469:parasitology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D060434:Herbivory; D006790:Host-Parasite Interactions; D008658:Inactivation, Metabolic; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*
D029681:Arabidopsis Proteins; D017382:Reactive Oxygen Species; C509522:BIK1 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases
Arabidopsis; detoxification enzyme; green peach aphid; hypersensitive response; plant-insect interaction