Water stress alters lignin content and related gene expression in two sugarcane genotypes.
The lignin deposition in the stem of two sugarcane genotypes was assessed on exposure to water stress. The lignin content and the morphoanatomical characterization of the stem indicated that IACSP94-2094 plants are more lignified than those of IACSP95-5000 genotype, under normal water supply conditions, which was especially associated with higher lignin contents in the rind of mature internodes. Water deficit had negative impact on the biomass production, mostly with IACSP94-2094 plants, possibly due to stress severity or higher susceptibility of that genotype during the stem-lengthening phase. Water deficit led to significant alterations in the expression levels of lignin biosynthesis genes and led to an approximate 60% increase of lignin content in the rind of young internodes in both genotypes. It is concluded that the young rind region was more directly affected by water stress and, depending on the genotype, a higher lignin accumulation may occur in the stem, thus implying lower quality biomass for bioethanol production.
†Departamento de Biologia Vegetal, Instituto de Biologia, CP 6109, Universidade Estadual de Campinas, CEP 13083-970 Campinas, SP, Brazil.|‡Embrapa Soja, Rodovia Carlos João Strass, s/n°, Acesso Orlando Amaral, Distrito de Warta, CP 231, CEP 86001-970 Londrina, PR, Brazil.
Adriana Brombini
AB
dos Santos
†Departamento de Biologia Vegetal, Instituto de Biologia, CP 6109, Universidade Estadual de Campinas, CEP 13083-970 Campinas, SP, Brazil.|§Centro de Tecnologia Canavieira (CTC), Rua Santo Antônio, Centro, CEP 13400-160 Piracicaba, SP, Brazil.
Alexandra
A
Bottcher
†Departamento de Biologia Vegetal, Instituto de Biologia, CP 6109, Universidade Estadual de Campinas, CEP 13083-970 Campinas, SP, Brazil.
Eduardo
E
Kiyota
†Departamento de Biologia Vegetal, Instituto de Biologia, CP 6109, Universidade Estadual de Campinas, CEP 13083-970 Campinas, SP, Brazil.
Juliana Lischka Sampaio
JL
Mayer
∥Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, CEP 13083-875 Campinas, SP, Brazil.
Renato
R
Vicentini
†Departamento de Biologia Vegetal, Instituto de Biologia, CP 6109, Universidade Estadual de Campinas, CEP 13083-970 Campinas, SP, Brazil.|⊥Centro de Cana, Instituto Agronômico de Campinas, CEP 14001-970 Ribeirão Preto, SP, Brazil.
Michael dos Santos
Mdos S
Brito
⊥Centro de Cana, Instituto Agronômico de Campinas, CEP 14001-970 Ribeirão Preto, SP, Brazil.
Silvana
S
Creste
⊥Centro de Cana, Instituto Agronômico de Campinas, CEP 14001-970 Ribeirão Preto, SP, Brazil.
Marcos G A
MG
Landell
†Departamento de Biologia Vegetal, Instituto de Biologia, CP 6109, Universidade Estadual de Campinas, CEP 13083-970 Campinas, SP, Brazil.
Paulo
P
Mazzafera
D018506:Gene Expression Regulation, Plant; D005838:Genotype; D008031:Lignin / Q000737:chemistry* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018547:Plant Stems / Q000737:chemistry / Q000378:metabolism; D031786:Saccharum / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014867:Water / Q000378:metabolism*
D010940:Plant Proteins; D014867:Water; D008031:Lignin
Saccharum; gene expression; genetic variability; lignocellulosic biomass; water stress