Intrahypothalamic Administration of Modafinil Increases Expression of MAP-Kinase in Hypothalamus and Pons in Rats.
Modafinil (MOD) it has to be considered as a wake-inducing drug to treat sleep disorders such as excessive sleepiness in narcolepsy, shift-work disorder, and obstructive/sleep apnea syndrome. Current evidence suggests that MOD induces waking involving the dopamine D1 receptor. However, little is known regarding the molecular elements linked in the wake-promoting actions of MOD. Since the D1 receptor activates the mitogen-activated protein kinase (MAP-K) cascade, it raises the interesting possibility that effects of MOD would depend upon the activation of MAP-K. Here we tested the expression of MAP-K in hypothalamus as well as pons after the microinjection of MOD (10 or 20 μg/1 μL) in rats into anterior hypothalamus, a wake-inducing brain area. Intrahypothalamic injections of MOD promoted MAP-K phosphorylation in hypothalamus and pons. Taken together, these results suggest that the wake-inducing compound MOD promotes the MAP-K phosphorylation.
Alwin
A
Poot-Ake
Stephanie
S
Mijangos-Moreno
Danielle
D
Manjarrez-Martin
Ramses
R
Jimenez-Moreno
Pedro R
PR
Aquino-Hernandez
Elda
E
Pacheco-Pantoja
Oscar
O
Arias-Carrion
Andrea
A
Sarro-Ramirez
Gloria
G
Arankowsky-Sandoval
Laboratorio de Neurociencias Moleculares e Integrativas. Escuela de Medicina, Division Ciencias de la Salud, Universidad Anahuac Mayab. Merida, Yucatan. Mexico. eric.murillo@anahuac.mx.
Eric
E
Murillo-Rodriguez
D000818:Animals; D001559:Benzhydryl Compounds / Q000008:administration & dosage*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D007031:Hypothalamus / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008845:Microinjections; D000077408:Modafinil; D010766:Phosphorylation / Q000187:drug effects; D011149:Pons / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D064690:Wakefulness-Promoting Agents / Q000008:administration & dosage*
D001559:Benzhydryl Compounds; D064690:Wakefulness-Promoting Agents; D048049:Extracellular Signal-Regulated MAP Kinases; D000077408:Modafinil