Carbohydrate-Based Nanocarriers Exhibiting Specific Cell Targeting with Minimum Influence from the Protein Corona.
Whenever nanoparticles encounter biological fluids like blood, proteins adsorb on their surface and form a so-called protein corona. Although its importance is widely accepted, information on the influence of surface functionalization of nanocarriers on the protein corona is still sparse, especially concerning how the functionalization of PEGylated nanocarriers with targeting agents will affect protein corona formation and how the protein corona may in turn influence the targeting effect. Herein, hydroxyethyl starch nanocarriers (HES-NCs) were prepared, PEGylated, and modified on the outer PEG layer with mannose to target dendritic cells (DCs). Their interaction with human plasma was then studied. Low overall protein adsorption with a distinct protein pattern and high specific affinity for DC binding were observed, thus indicating an efficient combination of "stealth" and targeting behavior.
Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz (Germany).
Biao
B
Kang
Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz (Germany).|Department of Hematology, Medical Oncology, and Pneumology, University Medical Center Mainz, Langenbeckstr. 1, 55131 Mainz (Germany).
Patricia
P
Okwieka
Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz (Germany).
Susanne
S
Schöttler
Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz (Germany).
Svenja
S
Winzen
Institute of Organic Chemistry, University of Mainz, Duesbergweg 10-14, 55128 Mainz (Germany).
Jens
J
Langhanki
Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz (Germany).
Kristin
K
Mohr
Institute of Organic Chemistry, University of Mainz, Duesbergweg 10-14, 55128 Mainz (Germany).
Till
T
Opatz
Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz (Germany).|Department of Hematology, Medical Oncology, and Pneumology, University Medical Center Mainz, Langenbeckstr. 1, 55131 Mainz (Germany).
Volker
V
Mailänder
Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz (Germany). landfester@mpip-mainz.mpg.de.
Katharina
K
Landfester
Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz (Germany). wurm@mpip-mainz.mpg.de.
Frederik R
FR
Wurm
D003713:Dendritic Cells / Q000378:metabolism*; D004337:Drug Carriers / Q000737:chemistry / Q000378:metabolism*; D016503:Drug Delivery Systems; D006801:Humans; D006895:Hydroxyethyl Starch Derivatives / Q000737:chemistry / Q000378:metabolism; D008358:Mannose / Q000737:chemistry / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000737:chemistry / Q000378:metabolism; D000066970:Protein Corona / Q000378:metabolism*
D004337:Drug Carriers; D006895:Hydroxyethyl Starch Derivatives; D000066970:Protein Corona; D011092:Polyethylene Glycols; D008358:Mannose
calorimetry; carbohydrates; drug delivery; nanoparticles; proteins