Characterization and antioxidant activity in vitro and in vivo of polysaccharide purified from Rana chensinensis skin.
Preliminary characterization and antioxidant activity in vitro and in vivo investigation of the polysaccharide fraction named as RCSP II, which was extracted from Rana chensinensis skin, were performed. Results indicated that RCSP II comprised glucose, galactose, and mannose in a molar ratio of 87.82:2.77:1.54 with a molecular weight of 12.8 kDa. Antioxidant activity assay in vitro showed that RCSP II exhibited 75.2% scavenging activity against 2,2'-azino-bis(3-ethylbenz-thiazoline-6-sulfonic acid) radicals at the concentration of 2500 mg/L and 85.1% against chelated ferrous ion at 4000 mg/L. Antioxidant activity assay in vivo further showed that RCSP II increased the activities of antioxidant enzymes, decreased the levels of malondialodehyde, and enhanced total antioxidant capabilities in livers and sera of d-galactose induced mice. These results suggested that RCSP II could have potential antioxidant applications as medicine or functional food.
College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning Shihua University, Fushun 113001, China. Electronic address: wangzy125@gmail.com.
Zhanyong
Z
Wang
College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning Shihua University, Fushun 113001, China.
Yuanyuan
Y
Zhao
College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning Shihua University, Fushun 113001, China.
Tingting
T
Su
College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning Shihua University, Fushun 113001, China.
Jing
J
Zhang
College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning Shihua University, Fushun 113001, China.
Fei
F
Wang
D000375:Aging / Q000187:drug effects; D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005690:Galactose / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000494:pharmacology; D005947:Glucose / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000494:pharmacology; D008358:Mannose / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000494:pharmacology; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D011898:Ranidae / Q000378:metabolism; D012867:Skin / Q000737:chemistry*
D000975:Antioxidants; D016166:Free Radical Scavengers; D011134:Polysaccharides; D005947:Glucose; D008358:Mannose; D005690:Galactose
Antioxidant activity; Polysaccharide; Rana chensinensis; Skin