Multifunctional biocompatible graphene oxide quantum dots decorated magnetic nanoplatform for efficient capture and two-photon imaging of rare tumor cells.
Circulating tumor cells (CTCs) are extremely rare cells in blood containing billions of other cells. The selective capture and identification of rare cells with sufficient sensitivity is a real challenge. Driven by this need, this manuscript reports the development of a multifunctional biocompatible graphene oxide quantum dots (GOQDs) coated, high-luminescence magnetic nanoplatform for the selective separation and diagnosis of Glypican-3 (GPC3)-expressed Hep G2 liver cancer tumor CTCs from infected blood. Experimental data show that an anti-GPC3-antibody-attached multifunctional nanoplatform can be used for selective Hep G2 hepatocellular carcinoma tumor cell separation from infected blood containing 10 tumor cells/mL of blood in a 15 mL sample. Reported data indicate that, because of an extremely high two-photon absorption cross section (40530 GM), an anti-GPC3-antibody-attached GOQDs-coated magnetic nanoplatform can be used as a two-photon luminescence platform for selective and very bright imaging of a Hep G2 tumor cell in a biological transparency window using 960 nm light. Experimental results with nontargeted GPC3(-) and SK-BR-3 breast cancer cells show that multifunctional-nanoplatform-based cell separation, followed by two-photon imaging, is highly selective for Hep G2 hepatocellular carcinoma tumor cells.
†Department of Chemistry and Biochemistry, Jackson State University, Jackson, Mississippi 39217, United States.
Yongliang
Y
Shi
†Department of Chemistry and Biochemistry, Jackson State University, Jackson, Mississippi 39217, United States.
Avijit
A
Pramanik
†Department of Chemistry and Biochemistry, Jackson State University, Jackson, Mississippi 39217, United States.
Christine
C
Tchounwou
‡Department of Physics and Astronomy, University of Texas at San Antonio, San Antonio, Texas 78249, United States.
Francisco
F
Pedraza
†Department of Chemistry and Biochemistry, Jackson State University, Jackson, Mississippi 39217, United States.
Rebecca A
RA
Crouch
†Department of Chemistry and Biochemistry, Jackson State University, Jackson, Mississippi 39217, United States.
Suhash Reddy
SR
Chavva
†Department of Chemistry and Biochemistry, Jackson State University, Jackson, Mississippi 39217, United States.
Aruna
A
Vangara
†Department of Chemistry and Biochemistry, Jackson State University, Jackson, Mississippi 39217, United States.
Sudarson Sekhar
SS
Sinha
†Department of Chemistry and Biochemistry, Jackson State University, Jackson, Mississippi 39217, United States.
Stacy
S
Jones
‡Department of Physics and Astronomy, University of Texas at San Antonio, San Antonio, Texas 78249, United States.
Dhiraj
D
Sardar
§Department of Chemistry and Biochemistry and Materials Research Laboratory, Materials Department, University of California at Santa Barbara, Santa Barbara, California 93106, United States.
Craig
C
Hawker
†Department of Chemistry and Biochemistry, Jackson State University, Jackson, Mississippi 39217, United States.
Paresh Chandra
PC
Ray
D001672:Biocompatible Materials / Q000138:chemical synthesis*; D006108:Graphite / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008422:Materials Testing; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D058726:Nanoconjugates / Q000737:chemistry / Q000648:ultrastructure; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D010087:Oxides / Q000737:chemistry; D010316:Particle Size; D045663:Quantum Dots; D035583:Rare Diseases / Q000473:pathology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D001672:Biocompatible Materials; D058185:Magnetite Nanoparticles; D058726:Nanoconjugates; D010087:Oxides; D006108:Graphite
graphene oxide quantum dots; highly efficient two-photon-absorbing material; luminescent magnetic nanoplatform; rare liver cancer cell separation from blood; selective two-photon imaging