Assessing and broadening genetic diversity of a rapeseed germplasm collection.
Assessing the level of genetic diversity within a germplasm collection contributes to evaluating the potential for its utilization as a gene pool to improve the performance of cultivars. In this study, 45 high-quality simple sequence repeat (SSR) markers were screened and used to estimate the genetic base of a world-wide collection of 248 rapeseed (Brassica napus) inbred lines. For the whole collection, the genetic diversity of A genome was higher than that of C genome. The genetic diversity of C genome for the semi-winter type was the lowest among the different germplasm types. Because B. oleracea is usually used to broaden the genetic diversity of C genome in rapeseed, we evaluated the potential of 25 wild B. oleracea lines. More allelic variations and a higher genetic diversity were observed in B. oleracea than in rapeseed. One B. oleracea line and one oilseed B. rapa line were used to generate a resynthesized Brassica napus line, which was then crossed with six semi-winter rapeseed cultivars to produce 7 F1 hybrids. Not only the allele introgression but also mutations were observed in the hybrids, resulting in significant improvement of the genetic base.
Key Laboratory of Oil Crops Biology and Genetic Improvement, Ministry of Agriculture, Oil Crops Research Institute, CAAS , Wuhan 430062 , China.
Jinfeng
J
Wu
Key Laboratory of Oil Crops Biology and Genetic Improvement, Ministry of Agriculture, Oil Crops Research Institute, CAAS , Wuhan 430062 , China.
Feng
F
Li
Key Laboratory of Oil Crops Biology and Genetic Improvement, Ministry of Agriculture, Oil Crops Research Institute, CAAS , Wuhan 430062 , China.
Kun
K
Xu
Key Laboratory of Oil Crops Biology and Genetic Improvement, Ministry of Agriculture, Oil Crops Research Institute, CAAS , Wuhan 430062 , China.
Guizhen
G
Gao
Key Laboratory of Oil Crops Biology and Genetic Improvement, Ministry of Agriculture, Oil Crops Research Institute, CAAS , Wuhan 430062 , China.
Biyun
B
Chen
Key Laboratory of Oil Crops Biology and Genetic Improvement, Ministry of Agriculture, Oil Crops Research Institute, CAAS , Wuhan 430062 , China.
Guixin
G
Yan
Key Laboratory of Oil Crops Biology and Genetic Improvement, Ministry of Agriculture, Oil Crops Research Institute, CAAS , Wuhan 430062 , China.
Nian
N
Wang
Key Laboratory of Oil Crops Biology and Genetic Improvement, Ministry of Agriculture, Oil Crops Research Institute, CAAS , Wuhan 430062 , China.
Jiangwei
J
Qiao
Key Laboratory of Oil Crops Biology and Genetic Improvement, Ministry of Agriculture, Oil Crops Research Institute, CAAS , Wuhan 430062 , China.
Jun
J
Li
Key Laboratory of Oil Crops Biology and Genetic Improvement, Ministry of Agriculture, Oil Crops Research Institute, CAAS , Wuhan 430062 , China.
Hao
H
Li
Key Laboratory of Oil Crops Biology and Genetic Improvement, Ministry of Agriculture, Oil Crops Research Institute, CAAS , Wuhan 430062 , China.
Tianyao
T
Zhang
Key Laboratory of Oil Crops Biology and Genetic Improvement, Ministry of Agriculture, Oil Crops Research Institute, CAAS , Wuhan 430062 , China.
Weiling
W
Song
Key Laboratory of Oil Crops Biology and Genetic Improvement, Ministry of Agriculture, Oil Crops Research Institute, CAAS , Wuhan 430062 , China.
Xiaoming
X
Wu
Brassica oleracea; SSR mutation; allelic variation; genetic diversity; resynthesized Brassica napus