MALDI-TOF mass spectrometry for early identification of bacteria grown in blood culture bottles.
This note reports an interesting way to rapidly identify bacteria grown from blood culture bottles. Chocolate agar plates were inoculated with 1 drop of the positive blood bottle medium. After a 3-hour incubation, the growth veil was submitted to MALDI-TOF mass spectrometry: 77% of the bacteria present have been correctly identified.
Laboratory of Bacteriology, University Hospital of Bordeaux, France.
Jean-Benoît
JB
Zabbe
Laboratory of Bacteriology, University Hospital of Bordeaux, France.
Laura
L
Zanardo
Laboratory of Bacteriology, University Hospital of Bordeaux, France.
Francis
F
Mégraud
Laboratory of Bacteriology, University Hospital of Bordeaux, France.
Emilie
E
Bessède
D001419:Bacteria / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques / Q000379:methods*; D001769:Blood / Q000382:microbiology; D003470:Culture Media / Q000378:metabolism; D006801:Humans; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
D003470:Culture Media
Bacteremia diagnosis; Rapid direct identification; Sepsityper alternative; Septic patients