Cytotoxicity of the diterpene 14-O-methyl-ryanodanol from Erythroxylum passerinum in an astrocytic cells model.
Plant secondary metabolites, such as, specifically, alkaloids and terpenes, may present psychoactive properties that modify the function of the central nervous system (CNS) and induce neurotoxicity. Neurotoxicity involves the response of glial cells, mainly astrocytes, which play a fundamental role in the control of homeostasis of the CNS. Some Erythroxylum species are indigenous to the state of Bahia in Brazil. This study investigated the cytotoxic activity of the diterpene AEP-1, extracted from the fruit of E. passerinum in a GL-15 cell line, astrocytic, glial cells model. The effects on cell viability, analyzed by the MTT assay, demonstrated a dose-dependent cytotoxic effect, with maximum effect at 500 μg/mL of AEP-1, and with a reduction of about 40 and 47% on cellular viability after 24 h and 72 h treatment, respectively. Evidence for induction of apoptosis by AEP-1 was first obtained when GL-15 glial cells were incubated with 250 μg/mL AEP-1 causing reniform and/or pyknotic nuclei and apoptotic bodies revealed by chromatin staining with Hoechst 33258. Increase in DNA fragmentation was also observed by comet assays in cells incubated with 500 μg/mL of AEP-1. Moreover, cells exposed to a sub toxic dose of AEP-1 (250 μg/mL) showed significant changes in morphology--contraction of the cytoplasm and expansion of cellular projections--signifying the presence of astrocytic cytoskeletal protein and glial fibrillary acidic protein (GFAP). These findings indicated astrocytic cells as the target for terpene AEP-1 and suggest the involvement of glial cells with psychoactive symptoms observed in humans and animals after consumption of fruits of plants of the genus Erythroxylum.
Noélio de Jesus
Nde J
Menezes-Filho
Cleide dos Santos
Cdos S
Souza
Tereza Cristina Silva
TC
Costa
Victor Diógenes Amaral
VD
Da Silva
Cátia Suse de Oliveira
CS
Ribeiro
Marizeth Liborio
ML
Barreiros
Jose Fernando Oliveira
JF
Costa
Jorge Mauricio
JM
David
Juceni P L
JP
David
Silvia Lima
SL
Costa
D017209:Apoptosis / Q000187:drug effects; D001253:Astrocytes / Q000166:cytology* / Q000187:drug effects; D001254:Astrocytoma / Q000503:physiopathology*; D045744:Cell Line, Tumor; D002470:Cell Survival; D053938:DNA Fragmentation / Q000187:drug effects; D004224:Diterpenes / Q000494:pharmacology*; D030921:Erythroxylaceae / Q000737:chemistry*; D006801:Humans; D008954:Models, Biological; D010936:Plant Extracts / Q000494:pharmacology*
C523742:14-O-methyl-ryanodanol; D004224:Diterpenes; D010936:Plant Extracts