Large-scale identification of secretome glycoproteins recognized by Wisteria floribunda agglutinin: A glycoproteomic approach to biomarker discovery.
One of the promising approaches to the development of cancer diagnostic systems is quantification of a specific protein carrying cancerous glycans. Potential utility of Wisteria floribunda agglutinin (WFA) for such assays has been suggested for several cancer types. To develop such diagnostic systems, identification of WFA-recognized glycoproteins is essential. Here, we successfully identified 504 WFA-recognized glycoproteins from the secretome of HEK293T cells. Most of the identified proteins were likely soluble or single-pass transmembrane proteins, which may serve as specific proteins for the diagnosis using biological fluids. Our method may help to discover marker glycoproteins for various cancers generating WFA-recognized glycans.
Research Center for Medical Glycoscience (RCMG), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.
Daisuke
D
Sugahara
Research Center for Medical Glycoscience (RCMG), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.
Azusa
A
Tomioka
Research Center for Medical Glycoscience (RCMG), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.
Takashi
T
Sato
Research Center for Medical Glycoscience (RCMG), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.
Hisashi
H
Narimatsu
Research Center for Medical Glycoscience (RCMG), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.
Hiroyuki
H
Kaji
D014408:Biomarkers, Tumor / Q000032:analysis / Q000378:metabolism; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid / Q000379:methods; D003470:Culture Media / Q000032:analysis; D054794:Glycomics / Q000379:methods; D006023:Glycoproteins / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D007785:Lactose / Q000031:analogs & derivatives / Q000737:chemistry; D008969:Molecular Sequence Data; D037121:Plant Lectins / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D034781:Receptors, N-Acetylglucosamine / Q000378:metabolism*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D014408:Biomarkers, Tumor; D003470:Culture Media; D006023:Glycoproteins; D037121:Plant Lectins; D034781:Receptors, N-Acetylglucosamine; C040129:wisteria lectin; C093701:N-acetylgalactosaminyl-1-4-N-acetylglucosamine; D007785:Lactose
Glycobiomarker; Glycoproteomics; IGOT; LC-MS; Protein glycosylation; Wisteria floribunda agglutinin