Chuanminshen violaceum polysaccharides improve the immune responses of foot-and-mouth disease vaccine in mice.
Water-soluble polysaccharides from Chuanminshen violaceum (CVPS) were evaluated for their physicochemical properties, monosaccharide composition, and adjuvant potential to specific cellular and humoral immune responses in a mouse model of foot-and-mouth disease virus (FMDV) vaccination. The average molecular weight (Mw) of the CVPS was 968.31 kDa. The monosaccharide components of the CVPS was rhamnose, arabinose, fucose, mannose, glucose, and galactose with a relative mass of 6.29%, 21.87%, 16.59%, 12.54%, 13.07%, and 28.05%, respectively. Administering CVPS as an adjuvant significantly enhanced the phagocytic capacity of peritoneal macrophages, splenocyte proliferation, and the activity of NK cells and CTL as well as increased FMDV-specific IgG and IgG subclass antibody titers. Moreover, CVPS increased the expression of IL-2, IFN-γ, and IL-4 in CD4(+) T cells and IFN-γ expression in CD8(+) T cells. Additionally, CVPS enhanced CD40(+), CD80(+), and CD86(+) expression on DCs. Moreover, CVPS upregulated MHC-I/II, TLR-2/4 mRNA levels. In contrast, CVPS downregulated TGF-β mRNA expression and the frequency of CD4(+)CD25(+)Foxp3(+) Treg cells. Taken together, these results indicate that administering CVPS as an adjuvant enhances both cellular and humoral immune responses via the TLR-2 and TLR-4 signalling pathways, thereby promoting DC maturation and suppressing TGF-β expression and Treg frequency.
Department of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, PR China. Electronic address: fenghaiborc@126.com.
Haibo
H
Feng
Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu, Sichuan 610051, PR China.
Jing
J
Fan
Department of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, PR China.
Hong
H
Qiu
Department of Animal and Veterinary Science, Chengdu Vocational College of Agricultural Science and Technology, WenJiang, Sichuan 611130, PR China.
Zhenhua
Z
Wang
Department of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, PR China.
Zhiqiang
Z
Yan
Department of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, PR China.
Lihua
L
Yuan
Department of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, PR China.
Lu
L
Guan
Applied Biophysics and Immune Engineering Laboratory, College of Life and Science, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR China.
Xiaogang
X
Du
Department of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, PR China.
Zhenhui
Z
Song
Department of Animal and Veterinary Science, Chengdu Vocational College of Agricultural Science and Technology, WenJiang, Sichuan 611130, PR China.
Xingfa
X
Han
Department of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, PR China. Electronic address: liujuanb@163.com.
Juan
J
Liu
D000276:Adjuvants, Immunologic; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D019661:Apiaceae / Q000737:chemistry*; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology; D004195:Disease Models, Animal; D000939:Epitopes / Q000276:immunology; D005260:Female; D005536:Foot-and-Mouth Disease / Q000235:genetics / Q000276:immunology* / Q000378:metabolism / Q000517:prevention & control; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D056724:Immunity, Humoral; D007074:Immunoglobulin G / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000276:immunology; D051379:Mice; D010587:Phagocytosis / Q000276:immunology; D011134:Polysaccharides / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D017550:Spectroscopy, Fourier Transform Infrared; D013154:Spleen / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D014765:Viral Vaccines / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D016207:Cytokines; D000939:Epitopes; D007074:Immunoglobulin G; D011134:Polysaccharides; D014765:Viral Vaccines
Adjuvant; Chuanminshen violaceum polysaccharide; FMDV