Sample preparation techniques coupled to advanced chromatographic methods for marine organisms investigation.
of this work was to develop suitable extraction methodologies for the isolation of lipids from fish, mussels and clams from the Mediterranean sea, and their successive analysis by means of advanced chromatographic instrumentation. More specifically, three different sample preparation methodologies were adopted: Folch's, Bligh & Dyer's and maceration. The lipidic extracts, after application of two different methylation procedures, were subjected to monodimensional and comprehensive two-dimensional GC analyses, in order to compare the fingerprints of samples derived from different extraction and transesterification methodologies. Triacylglycerols (TAGs) were analyzed by an off-line combination of silver-ion liquid chromatography with non-aqueous reversed phase liquid chromatography. In both LC and GC analyses, mass spectrometric detectors were used, which greatly supported the identification procedure. In particular, with respect to HPLC, mass spectrometry with atmospheric pressure chemical ionization in positive mode was applied.
"Scienze del Farmaco e Prodotti per la Salute" Department, University of Messina, Viale Annunziata, 98168 Messina, Italy.
Rosaria
R
Costa
"Scienze del Farmaco e Prodotti per la Salute" Department, University of Messina, Viale Annunziata, 98168 Messina, Italy.
Marco
M
Beccaria
"Scienze del Farmaco e Prodotti per la Salute" Department, University of Messina, Viale Annunziata, 98168 Messina, Italy.
Elisa
E
Grasso
Dipartimento di Scienze Biologiche e Ambientali (DiSBA), University of Messina, Viale Stagno d'Alcontres, 98166 Messina, Italy.
Ambrogina
A
Albergamo
"Scienze del Farmaco e Prodotti per la Salute" Department, University of Messina, Viale Annunziata, 98168 Messina, Italy.
Marianna
M
Oteri
"Scienze del Farmaco e Prodotti per la Salute" Department, University of Messina, Viale Annunziata, 98168 Messina, Italy; University Campus Bio-Medico of Rome, Via Álvaro del Portillo 21, 00128 Rome, Italy; Chromaleont s.r.l., c/o "Scienze del Farmaco e Prodotti per la Salute" Department, University of Messina, Viale Annunziata, 98168 Messina, Italy.
Paola
P
Dugo
Dipartimento di Scienze Biologiche e Ambientali (DiSBA), University of Messina, Viale Stagno d'Alcontres, 98166 Messina, Italy.
Salvatore
S
Fasulo
"Scienze del Farmaco e Prodotti per la Salute" Department, University of Messina, Viale Annunziata, 98168 Messina, Italy; University Campus Bio-Medico of Rome, Via Álvaro del Portillo 21, 00128 Rome, Italy; Chromaleont s.r.l., c/o "Scienze del Farmaco e Prodotti per la Salute" Department, University of Messina, Viale Annunziata, 98168 Messina, Italy. Electronic address: lmondello@unime.it.
Luigi
L
Mondello
D000818:Animals; D059001:Aquatic Organisms / Q000737:chemistry*; D002414:Cations, Monovalent / Q000737:chemistry; D002849:Chromatography, Gas / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D005399:Fishes / Q000378:metabolism; D008055:Lipids / Q000032:analysis / Q000302:isolation & purification*; D008522:Mediterranean Sea; D008974:Mollusca / Q000378:metabolism; D010938:Plant Oils; D012834:Silver / Q000737:chemistry; D014280:Triglycerides / Q000032:analysis / Q000302:isolation & purification
D002414:Cations, Monovalent; D008055:Lipids; D010938:Plant Oils; D014280:Triglycerides; D012834:Silver
Comprehensive gas chromatography; Fish; Molluscs; Non aqueous liquid chromatography; Silver ion liquid chromatography; Triacylglycerols