Casein kinase 1 (α, δ and ε) localize at the spindle poles, but may not be essential for mammalian oocyte meiotic progression.
CK1 (casein kinase 1) is a family of serine/threonine protein kinase that is ubiquitously expressed in eukaryotic organism. CK1 members are involved in the regulation of many cellular processes. Particularly, CK1 was reported to phosphorylate Rec8 subunits of cohesin complex and regulate chromosome segregation in meiosis in budding yeast and fission yeast. (1-3) Here we investigated the expression, subcellular localization and potential functions of CK1α, CK1δ and CK1ε during mouse oocyte meiotic maturation. We found that CK1α, CK1δ and CK1ε all concentrated at the spindle poles and co-localized with γ-tubulin in oocytes at both metaphase I (MI) and metaphase II (MII) stages. However, depletion of CK1 by RNAi or overexpression of wild type or kinase-dead CK1 showed no effects on either spindle organization or chromosome segregation during oocyte meiotic maturation. Thus, CK1 is not the kinase that phosphorylates Rec8 cohesin in mammalian oocytes, and CK1 may not be essential for spindle organization and meiotic progression although they localize at spindle poles.
a Key Laboratory of Major Obstetrics Diseases of Guangdong Province; The Third Affiliated hospital of Guangzhou Medical University ; Guangdong , China.
Shu-Tao
ST
Qi
Zhen-Bo
ZB
Wang
Lin
L
Huang
Li-Feng
LF
Liang
Ye-Xing
YX
Xian
Ying-Chun
YC
Ouyang
Yi
Y
Hou
Qing-Yuan
QY
Sun
Wei-Hua
WH
Wang
D000818:Animals; D015153:Blotting, Western; D047389:Casein Kinase I / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins; D005786:Gene Expression Regulation / Q000502:physiology*; D055785:Gene Knockdown Techniques; D008540:Meiosis / Q000502:physiology*; D051379:Mice; D018613:Microscopy, Confocal; D009687:Nuclear Proteins / Q000378:metabolism; D009865:Oocytes / Q000502:physiology*; D010750:Phosphoproteins / Q000378:metabolism; D021122:Protein Subunits / Q000378:metabolism; D064047:Spindle Poles / Q000378:metabolism*; D014404:Tubulin / Q000378:metabolism
D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; D010750:Phosphoproteins; D021122:Protein Subunits; C465863:Rec8 protein, mouse; D014404:Tubulin; D047389:Casein Kinase I
CK1; casein kinase 1; chromosome segregation; cohesion; meiosis; mouse; oocyte; rec8; spindle assembly