The transcriptional cofactor TRIM33 prevents apoptosis in B lymphoblastic leukemia by deactivating a single enhancer.
Most mammalian transcription factors (TFs) and cofactors occupy thousands of genomic sites and modulate the expression of large gene networks to implement their biological functions. In this study, we describe an exception to this paradigm. TRIM33 is identified here as a lineage dependency in B cell neoplasms and is shown to perform this essential function by associating with a single cis element. ChIP-seq analysis of TRIM33 in murine B cell leukemia revealed a preferential association with two lineage-specific enhancers that harbor an exceptional density of motifs recognized by the PU.1 TF. TRIM33 is recruited to these elements by PU.1, yet acts to antagonize PU.1 function. One of the PU.1/TRIM33 co-occupied enhancers is upstream of the pro-apoptotic gene Bim, and deleting this enhancer renders TRIM33 dispensable for leukemia cell survival. These findings reveal an essential role for TRIM33 in preventing apoptosis in B lymphoblastic leukemia by interfering with enhancer-mediated Bim activation.
Cold Spring Harbor Laboratory, New York, United States.
Eric
E
Wang
Cold Spring Harbor Laboratory, New York, United States.
Shinpei
S
Kawaoka
Cold Spring Harbor Laboratory, New York, United States.
Jae-Seok
JS
Roe
Cold Spring Harbor Laboratory, New York, United States.
Junwei
J
Shi
Cold Spring Harbor Laboratory, New York, United States.
Anja F
AF
Hohmann
Cold Spring Harbor Laboratory, New York, United States.
Yali
Y
Xu
Cold Spring Harbor Laboratory, New York, United States.
Anand S
AS
Bhagwat
Department of Medical Genome Sciences, University of Tokyo, Kashiwa, Japan.
Yutaka
Y
Suzuki
Cold Spring Harbor Laboratory, New York, United States.
Justin B
JB
Kinney
Cold Spring Harbor Laboratory, New York, United States.
Christopher R
CR
Vakoc
D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D001402:B-Lymphocytes / Q000378:metabolism* / Q000473:pathology; D000072224:Bcl-2-Like Protein 11; D001665:Binding Sites; D019070:Cell Lineage / Q000235:genetics; D002470:Cell Survival; D004742:Enhancer Elements, Genetic; D015973:Gene Expression Regulation, Leukemic; D053263:Gene Regulatory Networks; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D015452:Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D011485:Protein Binding; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D000099232:Proto-Oncogene Protein Spi-1
D051017:Apoptosis Regulatory Proteins; D000072224:Bcl-2-Like Protein 11; D008565:Membrane Proteins; D011518:Proto-Oncogene Proteins; D034741:RNA, Small Interfering; D015534:Trans-Activators; D014157:Transcription Factors; D000099232:Proto-Oncogene Protein Spi-1; C000606775:BCL2L11 protein, human; C000606776:Bcl2l11 protein, mouse; C491420:Trim33 protein, mouse
B cell; Bim; PU.1; Trim33; chromosomes; enhancer; genes; human; leukemia; mouse