Effects of recombinant bovine somatotropin during the periparturient period on innate and adaptive immune responses, systemic inflammation, and metabolism of dairy cows.
The aim of this experiment was to determine effects of treating peripartum dairy cows with body condition score ≥3.75 with recombinant bovine somatotropin (rbST) on immune, inflammatory, and metabolic responses. Holstein cows (253±1d of gestation) were assigned randomly to 1 of 3 treatments: untreated control (n=53), rbST87.5 (n=56; 87.5mg of rbST), and rbST125 (n=57; 125mg of rbST). Cows in the rbST87.5 and rbST125 treatments received rbST weekly from -21 to 28d relative to calving. Growth hormone, insulin-like growth factor 1, haptoglobin, tumor necrosis factor α, nonesterified fatty acids, β-hydroxybutyrate, glucose, and cortisol concentrations were determined weekly from -21 to 21d relative to calving. Blood sampled weekly from -14 to 21d relative to calving was used for hemogram and polymorphonuclear leukocyte (PMNL) expression of adhesion molecules, phagocytosis, and oxidative burst. Cows were vaccinated with ovalbumin at -21, -7, and 7d relative to calving, and blood was collected weekly from -21 to 21d relative to calving to determine IgG anti-ovalbumin concentrations. A subsample of cows had liver biopsied -21, -7, and 7d relative to calving to determine total lipids, triglycerides, and glycogen content. Growth hormone concentrations prepartum (control=11.0±1.2, rbST87.5=14.1±1.2, rbST125=15.1±1.3ng/mL) and postpartum (control=14.4±1.1, rbST87.5=17.8±1.2, rbST125=21.8±1.1ng/mL) were highest for rbST125 cows. Cows treated with rbST had higher insulin-like growth factor 1 concentrations than control cows (control=110.5±4.5, rbST87.5=126.2±4.5, rbST125=127.2±4.5ng/mL) only prepartum. Intensity of L-selectin expression was higher for rbST125 than for control and rbST87.5 cows [control=3,590±270, rbST87.5=3,279±271, rbST125=4,371±279 geometric mean fluorescence intensity (GMFI)] in the prepartum period. The PMNL intensities of phagocytosis (control=3,131±130, rbST87.5=3,391±133, rbST125=3,673±137 GMFI) and oxidative burst (control=9,588±746, rbST87.5=11,238±761, rbST125=12,724±781 GMFI) were higher for rbST125 cows than for control cows during the prepartum period. Concentrations of serum IgG anti-ovalbumin tended to be higher for rbST125 cows than for control cows (control=0.75±0.11, rbST87.5=0.94±0.10, rbST125=1.11±0.11 optical density) in the prepartum period. Haptoglobin concentration was significantly reduced 7d postpartum for rbST125 treatment compared with control and rbST87.5 treatments (control=2.74±0.28, rbST87.5=2.81±0.28, rbST125=1.87±0.28 optical density). Although treatment tended to affect postpartum β-hydroxybutyrate (control=747.5±40.2, rbST87.5=753.2±40.1, rbST125=648.8±39.7 µmol/L), it did not affect liver contents of total lipids, triglycerides, or glycogen. Incidence of metritis among rbST125 cows was reduced compared with that in control cows (control=23.1, rbST87.5=18.0, rbST125=7.8%). Treatment of dairy cows with 125mg of rbST improved innate immune responses and IgG concentration, with limited effects on metabolism.
Department of Veterinary Population Medicine, University of Minnesota, Saint Paul 55108; Department of Animal Science, University of Minnesota, Saint Paul 55108.
P R B
PR
Silva
Department of Veterinary Population Medicine, University of Minnesota, Saint Paul 55108.
K S
KS
Machado
Department of Animal Science, University of Minnesota, Saint Paul 55108.
D N Lobão
DN
Da Silva
Department of Veterinary Population Medicine, University of Minnesota, Saint Paul 55108; Department of Animal Science, University of Minnesota, Saint Paul 55108.
J G N
JG
Moraes
Division of Animal Science, University of Missouri, Columbia 65211.
D H
DH
Keisler
Department of Veterinary Population Medicine, University of Minnesota, Saint Paul 55108; Department of Animal Science, University of Minnesota, Saint Paul 55108. Electronic address: rcchebel@ufl.edu.
R C
RC
Chebel
D020155:3-Hydroxybutyric Acid / Q000097:blood; D056704:Adaptive Immunity / Q000276:immunology; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000276:immunology / Q000378:metabolism / Q000503:physiopathology*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D013006:Growth Hormone / Q000008:administration & dosage* / Q000097:blood; D006242:Haptoglobins / Q000032:analysis; D007249:Inflammation / Q000276:immunology / Q000378:metabolism / Q000662:veterinary*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D019041:L-Selectin / Q000378:metabolism; D009504:Neutrophils / Q000276:immunology; D010047:Ovalbumin / Q000276:immunology; D058725:Peripartum Period / Q000502:physiology; D010587:Phagocytosis / Q000276:immunology; D049590:Postpartum Period / Q000276:immunology; D016897:Respiratory Burst / Q000276:immunology
D005230:Fatty Acids, Nonesterified; D006242:Haptoglobins; D019041:L-Selectin; C073294:growth hormone, bovine; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone; D010047:Ovalbumin; D020155:3-Hydroxybutyric Acid
dairy cow; immune response; somatotropin