Potential cytotoxicity of water-soluble fraction of dust and particulate matters and relation to metal(loid)s based on three human cell lines.
Hepatocellular liver carcinoma (HepG2), human skin derived keratinocyte (KERTr,) and lung epithelial carcinoma (A549) were employed in MTT assay to evaluate the cytotoxicity of water-soluble fraction of road dust, air-conditioning (AC) filter dust and PM2.5 via ingestion, dermal contact and inhalation. Their effects on cell growth were dependent on exposure time and concentration. The LC20s of PM2.5 for A549 cell were approximately one order of magnitude lower than those of road dust and AC filter dust for KERTr cell and HepG2 cell. The LC20s of aqueous extracts were negatively correlated to the water-soluble metal(loid)s contained in dust coarse particles (KERTr: p=0.004; HepG2: p<0.001). However, no significant correlation between soluble metal(loid)s and LC20s of PM2.5 was observed for A549 cell (p>0.05). Other water-soluble components in dust and PM might cause the cell hazards synergistically or additively with metal(loid)s.
School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, PR China; Croucher Institute for Environmental Sciences, Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China; Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Sun Yat-sen University, Guangzhou 510275, PR China. Electronic address: mjuan_huang@hotmail.com.
Minjuan
M
Huang
Croucher Institute for Environmental Sciences, Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China; School of Chemistry & Environment, South China Normal University, Key Laboratory of Theoretical Chemistry of Environment, Ministry of Education, Higher Education Mega Center, Guangzhou 510006, PR China.
Yuan
Y
Kang
Croucher Institute for Environmental Sciences, Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China.
Wei
W
Wang
School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, PR China; Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Sun Yat-sen University, Guangzhou 510275, PR China.
Chuen Yu
CY
Chan
School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, PR China; Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Sun Yat-sen University, Guangzhou 510275, PR China.
Xuemei
X
Wang
Croucher Institute for Environmental Sciences, Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China. Electronic address: mhwong@hkbu.edu.hk.
Ming Hung
MH
Wong
D002460:Cell Line; D004391:Dust; D006801:Humans; D058955:Metalloids / Q000633:toxicity*; D052638:Particulate Matter / Q000633:toxicity*; D018675:Toxicity Tests
D004391:Dust; D058955:Metalloids; D052638:Particulate Matter
A549; Cytotoxicity; Dust and PM; HepG2; KERTr; Water-soluble metal(loid)s