Antibacterial activity of Lactobacillus spp. isolated from the feces of healthy infants against enteropathogenic bacteria.
Lactobacilli are normal microflora of the gastrointestinal (GI) tract and are a heterogeneous group of lactic acid bacteria (LAB). Lactobacillus strains with Probiotic activity may have health Benefits for human. This study investigates the probiotic potential of Lactobacillus strains obtained from the feces of healthy infants and also explores antibacterial activity of Lactobacillus strains with probiotic potential against enteropathogenic bacteria. Fecal samples were collected from 95 healthy infants younger than 18 months. Two hundred and ninety Lactobacillus strains were isolated and assessed for probiotic potential properties including ability to survive in gastrointestinal conditions (pH 2.0, 0.3% oxgall), adherence to HT-29 cells and antibiotic resistance. Six strains including Lactobacillus fermentum (4 strains), Lactobacillus paracasei and Lactobacillus plantarum showed good probiotic potential and inhibited the growth of enteropathogenic bacteria including ETEC H10407, Shigella flexneri ATCC 12022, Shigella sonnei ATCC 9290, Salmonella enteritidis H7 and Yersinia enterocolitica ATCC 23715. These Lactobacillus strains with probiotic potential may be useful for prevention or treatment of diarrhea, but further in vitro and in vivo studies on these strains are still required.
Div. of Bacteriology, Dept. of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Abolfazl
A
Davoodabadi
Div. of Bacteriology, Dept. of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: msoltandallal@gmail.com.
Mohammad Mehdi
MM
Soltan Dallal
Dept. of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Abbas
A
Rahimi Foroushani
Div. of Bacteriology, Dept. of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Masoumeh
M
Douraghi
Zoonosis Research Centre, Tehran University of Medical Sciences, Tehran, Iran; Dept. of Medical Laboratory Sciences, School of Para Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Mohammad Kazem
MK
Sharifi Yazdi
Div. of Bacteriology, Dept. of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Farzaneh
F
Amin Harati
D000898:Antibiosis; D001422:Bacterial Adhesion; D024881:Drug Resistance, Bacterial; D004847:Epithelial Cells / Q000382:microbiology; D005243:Feces / Q000382:microbiology*; D064368:Healthy Volunteers; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007223:Infant; D007778:Lactobacillus / Q000302:isolation & purification* / Q000502:physiology*; D050296:Microbial Viability / Q000187:drug effects; D012477:Salmonella enteritidis / Q000254:growth & development*; D012760:Shigella / Q000254:growth & development*; D015008:Yersinia enterocolitica / Q000254:growth & development*
Antibacterial; Enteropathogens; Infant; Lactobacillus; Probiotic