Preparation of fucoidan-shelled and genipin-crosslinked chitosan beads for antibacterial application.
In this study, a fucoidan-shelled chitosan bead was developed with the purpose of oral delivery of berberine to inhibit the growth of bacteria. The cross-linking level and swelling property of the beads were affected by the pH value and the composition of the genipin/fucoidan combined gelling agent. The drug release of the berberine-loaded beads was faster in simulated gastric fluid (pH 1.2) than those in simulated intestinal fluid (pH 7.4). Furthermore, a nanoparticles/beads complex system was developed by incorporation of berberine-loaded chitosan/fucoidan nanoparticles in the fucoidan-shelled chitosan beads. The nanoparticles/beads complex served as a drug carrier to delay the berberine release in simulated gastric fluid, with an estimated lag time of 2 h. Our results showed that the berberine-loaded beads and nanoparticles/beads complex could effectively inhibit the growth inhibition of common clinical pathogens, such as Staphylococcus aureus and Escherichia coli, and have the advantage of continually releasing berberine to inhibit the growth of the bacteria over 24 h.
Department of Materials Science and Engineering, Vanung University, Chung-Li, Taiwan.
Shu-Huei
SH
Yu
Department of Chemical Engineering, Ming Chi University of Technology, New Taipei City 243, Taiwan.
Shao-Jung
SJ
Wu
Department of Biochemistry and Molecular Cell Biology, School of Medicine, College of Medicine,Taipei Medical University, Taipei City 110, Taiwan; Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei City 110, Taiwan.
Jui-Yu
JY
Wu
Department of Biotechnology, Vanung University, Chung-Li, Taiwan.
De-Yu
DY
Wen
Department of Biochemistry and Molecular Cell Biology, School of Medicine, College of Medicine,Taipei Medical University, Taipei City 110, Taiwan; Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei City 110, Taiwan. Electronic address: flmi530326@tmu.edu.tw.
Fwu-Long
FL
Mi
D000284:Administration, Oral; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000494:pharmacology; D001599:Berberine / Q000008:administration & dosage* / Q000494:pharmacology; D048271:Chitosan / Q000031:analogs & derivatives*; D003432:Cross-Linking Reagents / Q000737:chemistry; D003692:Delayed-Action Preparations / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects; D004927:Escherichia coli Infections / Q000188:drug therapy; D006801:Humans; D039823:Iridoids / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D013203:Staphylococcal Infections / Q000188:drug therapy; D013211:Staphylococcus aureus / Q000187:drug effects
D000900:Anti-Bacterial Agents; D003432:Cross-Linking Reagents; D003692:Delayed-Action Preparations; D039823:Iridoids; D011134:Polysaccharides; D001599:Berberine; D048271:Chitosan; C007789:fucoidan; C007834:genipin
Antibacterial; Chitosan; Drug delivery; Fucoidan; Genipin