Catalpol protects mice against renal ischemia/reperfusion injury via suppressing PI3K/Akt-eNOS signaling and inflammation.
Renal ischemia/reperfusion-injury (IRI) is a common disease in clinic, which is also the most common cause of acute kidney failure. Previous investigations has illustrated that catalpol has neuroprotective, anti-inflammatory, and anti-hepatitis virus effects. This study was designed to investigate the protective effect of catalpol on renal IRI mice through suppressing phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt)-endothelial nitric oxide synthase (eNOS) and against inflammation, and the possible underlying mechanism. Firstly, we used renal IRI model to analyze blood urea nitrogen and serum creatinine levels in renal IRI mice. Next, real-time PCR and western blotting were used to detect the expression of KIM-1 and the expression of PI3K, Akt and eNOS levels in renal IRI, respectively. In addition, activities of tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6) and interleukin-10 (IL-10) in renal IRI mice were measured with respective TNF-α, IL-1β, IL-6 and IL-10 ELISA kits. Our results showed that catalpol clearly reduced blood urea nitrogen, serum creatinine levels and the expression of KIM-1 in renal IRI mice. Meanwhile, we found that catalpol markedly reduced the expression of PI3K, Akt and eNOS levels in renal IRI group. Suppressing of the PI3K/Akt-eNOS and the TNF-α, IL-1β, IL-6 and IL-10 activities was involved in the protective effect of catalpol on renal IRI. Collectively, catalpol protected renal IRI via inhibiting PI3K/Akt-eNOS signaling and inflammatory responses.
Department of Nephrology, Renmin Hospital of Wuhan University Wuhan 430060, Hubei, P. R. China.
Jili
J
Zhu
Department of Nephrology, Renmin Hospital of Wuhan University Wuhan 430060, Hubei, P. R. China.
Xinghua
X
Chen
Department of Nephrology, Renmin Hospital of Wuhan University Wuhan 430060, Hubei, P. R. China.
Huiming
H
Wang
Department of Nephrology, Renmin Hospital of Wuhan University Wuhan 430060, Hubei, P. R. China.
Qi
Q
Yan
Catalpol; PI3K/Akt; eNOS; inflammation; renal ischemia/reperfusion-injury