Fully resolved NMR correlation spectroscopy.
A new correlation experiment cited as "push-G-SERF" is reported. In the resulting phased 2D spectrum, the chemical shift information is selected along the direct dimension, whereas scalar couplings involving a selected proton nucleus are edited in the indirect domain. The robustness of this pulse sequence is demonstrated on compounds with increasing structural and spectral complexity, using state-of-the-art spectrometers. It allows for full resolution of both dimensions of the spectrum, yielding a straightforward assignment and measurement of the coupling network around a given proton in the molecule. This experiment is intended for chemists who want to address efficiently the structural analysis of molecules with an overcrowded spectrum.
Equipe de RMN en milieu orienté Université Paris-Sud, ICMMO, UMR 8182 (CNRS-UPS), 91405 Orsay cedex (France) http://www.icmmo.u-psud.fr.
Daisy
D
Pitoux
Equipe de RMN en milieu orienté Université Paris-Sud, ICMMO, UMR 8182 (CNRS-UPS), 91405 Orsay cedex (France) http://www.icmmo.u-psud.fr.
Bertrand
B
Plainchont
Equipe de RMN en milieu orienté Université Paris-Sud, ICMMO, UMR 8182 (CNRS-UPS), 91405 Orsay cedex (France) http://www.icmmo.u-psud.fr.
Denis
D
Merlet
Equipe Méthodologies, Synthèses et Molécules Thérapeutiques, Université Paris-Sud, ICMMO, UMR 8182 (CNRS-UPS), LabEx LERMIT, 91405 Orsay Cedex (France).|Present address: Unité de Chimie des Biomolécules, Institut Pasteur, UMR CNRS 3523, 75724 Paris cedex (France).
Zhaoyu
Z
Hu
Equipe Méthodologies, Synthèses et Molécules Thérapeutiques, Université Paris-Sud, ICMMO, UMR 8182 (CNRS-UPS), LabEx LERMIT, 91405 Orsay Cedex (France).
David
D
Bonnaffé
Equipe de RMN en milieu orienté Université Paris-Sud, ICMMO, UMR 8182 (CNRS-UPS), 91405 Orsay cedex (France) http://www.icmmo.u-psud.fr.
Jonathan
J
Farjon
Equipe de RMN en milieu orienté Université Paris-Sud, ICMMO, UMR 8182 (CNRS-UPS), 91405 Orsay cedex (France) http://www.icmmo.u-psud.fr. nicolas.giraud@u-psud.fr.
Nicolas
N
Giraud
NMR spectroscopy; oligosaccharides; pure shift; selective refocusing; structure elucidation