Electron Transfer Flavoprotein Subunit Beta Is a Candidate Endothelial Cell Autoantigen in Behçet's Disease.
Behçet's disease (BD) is a chronic inflammatory disease with multisystem involvement, and it is listed as a rare disease in the United States but is common in the Middle East, China, and Japan. The aim of this study was to identify novel autoantigens in Chinese patients with BD. First, the candidate autoantigens were screened by Western blotting, and the sequences of putative antigens were identified by LC-MALDI-TOF/TOF mass spectrometry. Next, the screened protein was cloned, expressed and purified. Then, an optimized ELISA was developed, and the serological criteria were evaluated using a large number of confirmed patients. One antigen with a molecular weight of approximately 28 kDa was identified as electron transfer flavoprotein subunit beta (ETFB). Positive reactivity was detected in recombinant human ETFB sera from 38 of 92 BD patients (41 %) and 1 of 90 healthy controls (1 %).
School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
Peng
P
Chen
School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
Weikang
W
Yang
Department of Clinical Biochemistry, Chinese PLA General Hospital, Beijing, 100853, China.
Yaping
Y
Tian
Core Facility, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
Shutao
S
Sun
ImmunoHunt Corporation, 139 Fengtai Rd, Beijing, 100071, China.
Guangyu
G
Chen
Department of Clinical Biochemistry, Chinese PLA General Hospital, Beijing, 100853, China.
ChunYan
C
Zhang
School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
Fuxin
F
Ma
School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
Yiping
Y
Xun
School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
Lili
L
Shi
School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
Chunhe
C
Yang
School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
Lanqing
L
Zhao
School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
Yabin
Y
Zhou
School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
Hongwu
H
Du
D000293:Adolescent; D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D001324:Autoantigens / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D001528:Behcet Syndrome / Q000175:diagnosis / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D044927:Electron-Transferring Flavoproteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D042783:Endothelial Cells / Q000276:immunology* / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D055815:Young Adult
D001324:Autoantigens; C500319:ETFB protein, human; D044927:Electron-Transferring Flavoproteins; D010446:Peptide Fragments