Screening and analysis of Hc-ubq and Hc-gst related to desiccation survival of infective Haemonchus contortus larvae.
Infective Haemonchus contortus larvae (L3s) are able to protect themselves from desiccation. To explore the molecular mechanisms of desiccation survival, mRNA differential display RT-PCR was used to screen differentially expressed genes in L3s upon desiccation, followed by RNAi experiments to define gene functions. In this, 58 differentially expressed transcripts were obtained. Among these, the BF-U01A and CH-U02A fragments represent genes with the highest identity percentage in bioinformatic analysis. They were named Hc-ubq and Hc-gst based on their respective homologous ubiquitin in Caenorhabditis elegans and glutathione S-transferase in H. contortus. Quantitive RT-PCR results indicated that they were both up-regulated in desiccated L3s. Hc-ubq and Hc-gst RNAi in H. contortus showed reduced survival rate of L3s, with unchanged locomotion behavior. Homologous Ce-ubq-2 and Ce-gst-7 RNAi in C. elegans also displayed higher larval death rate. These results suggest that ubq and gst may play important roles in nematode desiccation tolerance. Our study analyzed desiccation resistance related genes in H. contortus L3s, and revealed significant research implications on the mechanisms behind nematode desiccation survival.
Institute of Preventive Veterinary Medicine & Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310058, China.
Yi
Y
Yang
Institute of Preventive Veterinary Medicine & Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310058, China.
Yujie
Y
Ma
Institute of Preventive Veterinary Medicine & Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310058, China.
Xueqiu
X
Chen
Institute of Preventive Veterinary Medicine & Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310058, China.
Xiaolu
X
Guo
Wenzhou Medical University, Wenzhou, 325035, China.
Baolong
B
Yan
Institute of Preventive Veterinary Medicine & Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310058, China. Electronic address: afdu@zju.edu.cn.
Aifang
A
Du
D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000502:physiology*; D019295:Computational Biology; D018509:DNA, Helminth / Q000235:genetics; D003890:Desiccation; D005260:Female; D020869:Gene Expression Profiling; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D006189:Haemonchus / Q000235:genetics / Q000502:physiology*; D015801:Helminth Proteins / Q000235:genetics* / Q000378:metabolism; D007814:Larva; D034622:RNA Interference; D025801:Ubiquitin / Q000235:genetics / Q000378:metabolism
D018509:DNA, Helminth; D015801:Helminth Proteins; D025801:Ubiquitin; D005982:Glutathione Transferase
Caenorhabditis elegans; Desiccation survival; Infective Haemonchus contortus larvae; RNAi; mRNA differential display; ubq and gst