[Studies on effects of calycosin-7-O-β-D-glucoside on prim-O-glucosylcimifugin and cimifugin in vivo pharmacokinetics].
Study on the effects of Astragali Radix main active flavone calycosin-7-O-β-D-glucoside on Saposhnikoviae Radix main active ingredients prim-O-glucosylcimifugin and cimifugin, a UPLC-MS/MS method for simultaneous determination of prim-O-glucosylcimifugin and cimifugin in rat plasma was established, and the comparative pharmacokinetics of prim-O-glucosylcimifugin and cimifugin after oral administration of prim-O-glucosylcimifugin and calycosin-7-O-β-D-glucoside-prim-O-glucosylcimifugin to rats were carried out, which might be conductive in exploring the rationality of Astragali Radix - Saposhnikoviae Radix herb couple. Twelve male SD rats were divided into two groups. Prim-O-glucosylcimifugin and cimifugin in rat plasma of different time points after oral administration of prim-O-glucosylcimifugin and calycosin-7-O-β-D-glucoside - prim-O-glucosylcimifugin to rats were determinated. And the main pharmacokinetic parameters were investigated using DAS 3. 2. 4. The established method was rapid, accurate and sensitive for simultaneous determination of prim-O-glucosylcimifugin and cimifugin in rat plasma. The analysis was performed on a Waters Acquity BEH C18 column (2.1 mm x 100 mm, 1.7 μm) with the mixture of acetonitrile and 0.1% formic acid/water as mobile phase, and the gradient elution at a flow rate of 0.3 mL x min(-1). The analytes were detected by tandem mass spectrometry with the electrospray ionization (ESI) source and in the multiple reaction monitoring (MRM) mode. Compared with prim-O-glucosylcimifugin group, the AUC(0-t)., and AUC(0-∞) of p-O-glucosylcimifugin as well as the C(max) of cimifugin significantly increased (P < 0.05) in calycosin-7-O-β-D-glucoside-prim-O-glucosylcimifugin group. Calycosin-7-O-β-D-glucoside could enhance the absorption of prim-O-glucosylcimifugin and cimifugin and improve the bioavailability, explaining preliminarily the rationality of Astragali Radix-Saposhnikoviae Radix herb couple.
Xiao-Li
XL
Zhao
Ling
L
Liu
Liu-Qing
LQ
Di
Jun-Song
JS
Li
An
A
Kang
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002867:Chromones / Q000097:blood / Q000493:pharmacokinetics*; D004347:Drug Interactions; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics*; D005960:Glucosides / Q000097:blood / Q000494:pharmacology*; D007529:Isoflavones / Q000097:blood / Q000494:pharmacology*; D008297:Male; D009005:Monosaccharides / Q000097:blood / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014966:Xanthenes / Q000097:blood / Q000493:pharmacokinetics*
D002867:Chromones; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D007529:Isoflavones; D009005:Monosaccharides; D014966:Xanthenes; C526426:calycosin-7-O-beta-D-glucoside; C533198:cimifugin; C498945:prim-O-glucosylcimifugin