A naphthalene diimide dyad for fluorescence switch-on detection of G-quadruplexes.
A non-fluorescent naphthalene diimide (NDI) dimer, conjugating red and blue NDI dyes, becomes red/NIR emitting upon G-quadruplex binding. The fluorescence lifetime which is significantly different for the complexes, the G-quadruplex/dimer and the weakly emitting ds-DNA/dimer is the key feature for the development of new rationally engineered G-quadruplex sensors.
Dipartimento di Chimica, Università di Pavia, V. le Taramelli 10, 27100 Pavia, Italy. mauro.freccero@unipv.it.
F
F
Doria
A
A
Oppi
F
F
Manoli
S
S
Botti
N
N
Kandoth
V
V
Grande
I
I
Manet
M
M
Freccero
D004396:Coloring Agents / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D019281:Dimerization; D005453:Fluorescence; D054856:G-Quadruplexes; D007094:Imides / Q000737:chemistry*; D009281:Naphthalenes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D004396:Coloring Agents; D007094:Imides; D009281:Naphthalenes; C542131:naphthalenediimide; D004247:DNA