[Epitope analysis of the hemagglutinin molecule of the Victoria lineage influenza B viruses].
A panel of five monoclonal antibodies (MAbs) to the HA1 molecule of the influenza B virus of the Victorian lineage with high virus-neutralizing activity was developed. For identification of the virus neutralizing epitopes in HA1 escape mutants (EM) of the influenza BIShandong/07/97 and B/Malaysia/2506/04 virus were selected using virus- neutralizing antibodies (MAbs). Three EMs had single, two--double and one--triple amino acid substitutions (AAS) in HA1 (H122N, A202E, K203T, K2031, K203N or A317V). In addition, AAS N197S was detected in three EMs. A correlation of AAS identified with peculiarities of interaction of EMs with Mabs was discussed.
E V
EV
Sorokin
T R
TR
Tsareva
A A
AA
Sominina
M M
MM
Pisareva
A V
AV
Komissarov
A A
AA
Kosheleva
M P
MP
Grudinin
D019943:Amino Acid Substitution; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification; D057134:Antibodies, Neutralizing / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification; D000936:Antigen-Antibody Complex / Q000032:analysis / Q000737:chemistry / Q000276:immunology; D000939:Epitopes / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D057131:Immune Evasion; D009981:Influenza B virus / Q000235:genetics / Q000276:immunology*; D007251:Influenza, Human / Q000276:immunology / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D008958:Models, Molecular
D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D000936:Antigen-Antibody Complex; D000939:Epitopes; D019267:Hemagglutinin Glycoproteins, Influenza Virus