[Immortalization of human dental pulp stem cells caused by transferring hTERT gene].
OBJECTIVE
To establish an immortalized human dental pulp stem cell line used for basic and clinical research of oral science.


METHODS
Human telomerase reverse transcriptase (hTERT) cDNA was transferred into human dental pulp stem cells (hDPSCs) by lentivirus. The resultant stable clones reproduced successively and the expression of hTERT was identified.


RESULTS
The hTERT gene was transferred into human dental pulp stem cells successfully. The transformed cells expressed telomerase activity and divided vigorously. p35 had been obtained so far.


CONCLUSIONS
The hDPSCs can be immortalized by transferring exogenous hTERT gene to constitute telomerase activity.
Department of Endodontics, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine. Shanghai 200011;China.E-mail:1127239423@sjtu.edu.cn.
Li
L
Gao
Yu-xia
YX
Wang
Wen-xin
WX
Jiang
Zhi-yan
ZY
He
Cai-lian
CL
Zhu
Rui
R
Ma
D053687:Adult Stem Cells; D002460:Cell Line; D002461:Cell Line, Transformed; D003782:Dental Pulp; D006801:Humans; D013234:Stem Cells; D019098:Telomerase
C509186:TERT protein, human; D019098:Telomerase