Identifying a potential receptor for the antibacterial peptide of sponge Axinella donnani endosymbiont.
Marine sponges and their associated bacteria are rich sources of novel secondary metabolites with therapeutic usefulness. In our earlier work, we have identified a novel antibacterial peptide from the marine sponge Axinella donnani endosymbiotic bacteria. In this work, we have carried out a comparative genomic analysis and identified a set of 60 proteins as probable receptor which is common in all the strains. The analysis on binding substrate showed that β barrel assembly machinery (BamA) of the outer membrane protein 85 (omp85) superfamily is a potential receptor protein for the antibacterial peptide. It plays a central role in OMP biogenesis, especially in cell viability. Further, the triplet and quartet motifs RGF and YGDG, respectively in L6 loop are conserved over all the strains and these conserved residues interact with antibacterial peptide to inhibit the BamA function, which is essential for OMP biogenesis.
Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600036 Tamilnadu, India. Electronic address: vimalabioinformatics@gmail.com.
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Vimala
Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600036 Tamilnadu, India.
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Ramakrishnan
Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600036 Tamilnadu, India. Electronic address: gromiha@iitm.ac.in.
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Gromiha
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism*; D010802:Phylogeny; D011161:Porifera / Q000737:chemistry* / Q000382:microbiology; D011956:Receptors, Cell Surface / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013559:Symbiosis
D000900:Anti-Bacterial Agents; D010455:Peptides; D011956:Receptors, Cell Surface
Antibacterial peptide; BamA; Endosymbiotic bacteria; OMP biogenesis; Omp85 super family