[Primary cutaneous diffuse large B-cell lymphoma, leg type: a study of clinicopathology, immunophenotype and gene rearrangement].
OBJECTIVE
To study the clinicopathologic features, immunophenotype and gene rearrangement of primary cutaneous diffuse large B-cell lymphoma, leg type (PCLBCL).


METHODS
Seven cases of PCLBCL were enrolled into the study. Clinicopathologic analysis, immunohistochemical staining and gene rearrangement for IgH and Igκ were undertaken in the study.


RESULTS
All the seven cases were male, and the median age was 72 years. Patients usually presented with multiple purple tumors, nodules, papules and infiltrative plaques. Two patients had a history of leg injury before onset, and one had mosquito bites. Histologically, the tumor involved the dermis and subcutis with dense and diffuse infiltrative pattern composing of centroblasts and/or immunoblasts. Immunohistochemical staining showed that seven cases (7/7) expressed CD20, six (6/6) expressed bcl-2, four (4/4) expressed MUM-1, four (4/5) expressed CD79a, four (4/5) expressed PAX-5 and four (4/6) expressed bcl-6, respectively. All cases did not express CD3ε, CD45RO, CD10 and CD30. IgH gene rearranged bands were detected in three (3/6) cases and Igκ was detected in one (1/5) case. Six of the seven cases died and the remaining patient, who was 44-year-old, was alive after 22 months of follow-up.


CONCLUSIONS
PCLBCL is rare, predominantly affects elderly male patients. PCLBCL has poor prognosis and high mortality, but younger patients seem to have better prognosis. Some cases had a history of trauma or mosquito bites. The relationship between the history and the onset of PCLBCL needs further evaluation.
Department of Dermatovenereology, West China Hospital, Sichuan University, Chengdu 610041, China.
Tingting
T
Wang
Ling
L
Jia
Wenjun
W
Liao
Liuqing
L
Chen
Xixue
X
Chen
Ya
Y
Xiong
Fei
F
Hao
Xuejun
X
Zhu
Xichuan
X
Yang
E-mail: lkzwl @126.com.
Lin
L
Wang
D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D009033:Culicidae; D015321:Gene Rearrangement; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007145:Immunoglobulin kappa-Chains / Q000235:genetics; D016130:Immunophenotyping; D007299:Insect Bites and Stings / Q000150:complications; D007866:Leg; D007869:Leg Injuries / Q000150:complications; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D051560:Proto-Oncogene Proteins c-bcl-6 / Q000378:metabolism; D012878:Skin Neoplasms / Q000235:genetics* / Q000473:pathology*
D015703:Antigens, CD; D007143:Immunoglobulin Heavy Chains; D007145:Immunoglobulin kappa-Chains; D051560:Proto-Oncogene Proteins c-bcl-6