Complete genome sequence of a porcine astrovirus from South Korea.
Porcine astrovirus (PAstV) is broadly distributed in pigs in several countries worldwide. PAstVs belong to the genus Mamastrovirus and are divided into five genetically divergent types. This study presents a molecular characterization of PAstV identified in diarrheic piglets in South Korea. The complete genome of the Korean PAstV strain KOR/KNU14-07/2014 was sequenced and analyzed to characterize PAstV circulating in South Korea. The full-length genomic sequence of KNU14-07 was determined to be 6,337 nucleotides in length and consisted of three major open reading frames (5'-ORF1a-ORF1b-ORF2-3'). The overall degree of nucleotide sequence identity was 40.8 to 72.5% between KUN14-07 and other reported PAstVs, indicating high heterogeneity among PAstVs. Genetic and phylogenetic analyses showed that the KNU14-07 strain was most closely related to the PAstV2 lineage, which is the second most common type in South Korea.
Animal Virology Laboratory, School of Life Sciences, College of Natural Sciences, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Daegu, 702-701, Republic of Korea.
Sunhee
S
Lee
Guehwan
G
Jang
Changhee
C
Lee
D000818:Animals; D019350:Astroviridae Infections / Q000662:veterinary* / Q000821:virology; D001483:Base Sequence; D016679:Genome, Viral; D001257:Mamastrovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D056910:Republic of Korea; D034421:Sus scrofa; D013552:Swine; D013553:Swine Diseases / Q000821:virology*