Protection conferred by virus-like particle vaccines against respiratory syncytial virus infection in mice by intranasal vaccination.
Respiratory syncytial virus (RSV) is a major pathogen in infants and the elderly, causing pneumonia and bronchiolitis. Despite decades of research, to date there is still no approved RSV vaccine available. In this study, we developed RSV virus-like particle (VLP) vaccines containing an RSV fusion (F) and/or attachment (G) protein with Newcastle disease virus (NDV) as the platform. The VLPs were expressed in a baculovirus system and purified by sucrose gradient centrifugation. BALB/c mice immunized intranasally (i.n.) with rNDV/RSV/F plus rNDV/RSV/G developed robust humoral, mucosal RSV-specific antibodies and cellular immune responses. Furthermore, rNDV/RSV/F plus rNDV/RSV/G provided better protection than did rNDV/RSV/F or rNDV/RSV/G alone, as shown by an obvious decrease in viral replication together with alleviation of histopathological changes in the lungs of the challenged mice. Our data demonstrate that the intranasal vaccination of combined RSV virus-like particle vaccine candidates has great potential for protection against RSV infection.
a Beijing Institute of Microbiology and Epidemiology; State Key Laboratory of Pathogen and Biosecurity Beijing , China.
Hongjing
H
Gu
Tieling
T
Li
Lina
L
Han
Ping
P
Zhu
Peirui
P
Zhang
Shaogeng
S
Zhang
Sujing
S
Sun
Yueqiang
Y
Duan
Li
L
Xing
Zhongpeng
Z
Zhao
Chengcai
C
Lai
Bohai
B
Wen
Xiliang
X
Wang
PengHui
P
Yang
D000281:Administration, Intranasal; D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology; D002460:Cell Line; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology / Q000517:prevention & control*; D022261:Respiratory Syncytial Virus Vaccines / Q000008:administration & dosage* / Q000276:immunology* / Q000627:therapeutic use; D058425:Vaccines, Virus-Like Particle / Q000276:immunology*
D057134:Antibodies, Neutralizing; D022261:Respiratory Syncytial Virus Vaccines; D058425:Vaccines, Virus-Like Particle
RSV; RSV virus-like particle vaccine; intranasal vaccination