Impact of GLP-1 analogue on oxidative damage and hepatic regeneration in experimental 70% hepatectomy model.
OBJECTIVE
The purpose of our study is researching into impact of glucagon like peptide 1 (GLP 1) analogue on liver regeneration after major hepatectomy.


METHODS
24 wistar albino rats were consecutively divided into 3 groups. Group 1: Control (sham) group day 14 (n = 8), Group 2: Liver resection group day 14 (n = 8); 70% Liver resection was performed, Group 3: Study group day 14 (n = 8); Subsequent to performing 70% liver resection, GLP-1 analogue was administered 2 times a day. (10 μgr/70 kg x 2 times). After 14 day, rats were sacrificed. Oxi- dative stress and antioxidant enzymes and mitochon- drial permeability transition, cytochrome-c, Bax, Bcl- 2, caspase-3, caspase-8 and caspase-3 activity were examined.


RESULTS
70% Liver resection induced oxi- dative stress of liver tissue was ameliorated by GLP-1 induction. Administration of GLP increased Bcl-2 ex- pression. Decreased expression of cytochrome-c was accompanied by a decrease caspase-3, caspase-8, and Bax expression and caspase-3 activity.


CONCLUSIONS
Glp 1 induction plays a regenerative role in the major hepatectomy. This effect is dependent on modulation of the antiapoptotic and antioxidative pathways by GLP 1 expression.
Muharrem
M
Battal
Bulent
B
Çitgez
Abdulcabbar
A
Kartal
Ahu
A
Kemik
Pinar
P
Yildirim
Yasar
Y
Ozdenkaya
Ahmet
A
Yilmaz
Oguzhan
O
Karatepe
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism; D052216:Glucagon-Like Peptide 1 / Q000031:analogs & derivatives / Q000494:pharmacology*; D006498:Hepatectomy / Q000379:methods*; D054795:Incretins / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D008115:Liver Regeneration / Q000187:drug effects*; D008297:Male; D023421:Models, Animal; D018384:Oxidative Stress / Q000187:drug effects*; D017208:Rats, Wistar; D013997:Time Factors
D000975:Antioxidants; D015415:Biomarkers; D054795:Incretins; D052216:Glucagon-Like Peptide 1; C505410:Casp3 protein, rat; D053148:Caspase 3