High-throughput genotyping of high-risk Human Papillomavirus by MALDI-TOF Mass Spectrometry-based method.
A high-throughput matrix-assisted laser desorption ionisation-time of flight (MALDI-TOF) mass spectrometry (MS)-based method was here developed to genotype 16 high-risk human papillomavirus (HPV) types in cervical cytology specimens. This method was compared to a commercial kit, the Inno-LiPA HPV genotyping assay, which detects a broad spectrum of HPV types. HPV DNA was assessed by the two methods in a total of 325 cervical cytology specimens collected in PreservCyt® solution. The overall agreement was almost perfect (Cohen's k=0.86) in term of positive and negative cases. Indeed, HPV types 16, 35, 56 and 66 showed the highest agreement values (>0.80). The highest agreement values (K >0.80) were found for all 16 HPV types in single infections, but only for HPV 16, 35, 45 and 56 in multiple infections. In conclusion, the high-throughput MS-based method developed here is well-suited for broad spectrum HPV genotyping in large-scale epidemiological studies.
Department of Experimental, Diagnostic and Specialty Medicine, University Hospital S. Orsola Malpighi, Via Massarenti 9, 40138, Bologna, Italy.
Monica
M
Cricca
Center for Applied Biomedical Research - CRBA, University Hospital S.Orsola-Malpighi, Via Massarenti 9, 40138, Bologna, Italy.
Elena
E
Marasco
Department of Experimental, Diagnostic and Specialty Medicine, University Hospital S. Orsola Malpighi, Via Massarenti 9, 40138, Bologna, Italy.
Federica
F
Alessandrini
Center for Applied Biomedical Research - CRBA, University Hospital S.Orsola-Malpighi, Via Massarenti 9, 40138, Bologna, Italy.
Chiara
C
Fazio
Center for Applied Biomedical Research - CRBA, University Hospital S.Orsola-Malpighi, Via Massarenti 9, 40138, Bologna, Italy.
Anna
A
Prossomariti
Department of Experimental, Diagnostic and Specialty Medicine, University Hospital S. Orsola Malpighi, Via Massarenti 9, 40138, Bologna, Italy.
Claudia
C
Savini
Department of Hematology, Oncology and Laboratory Medicine, Unit of Microbiology, University Hospital S.Orsola-Malpighi, Via Massarenti 9, 40138, Bologna, Italy.
Simona
S
Venturoli
Center for Applied Biomedical Research - CRBA, University Hospital S.Orsola-Malpighi, Via Massarenti 9, 40138, Bologna, Italy.
Pasquale
P
Chieco
Department of Experimental, Diagnostic and Specialty Medicine, University Hospital S. Orsola Malpighi, Via Massarenti 9, 40138, Bologna, Italy.
Sabrina
S
De Carolis
Department of Experimental, Diagnostic and Specialty Medicine - Center for Applied Biomedical Research - CRBA, University Hospital S.Orsola-Malpighi, Via Massarenti 9, 40138, Bologna, Italy.
Massimiliano
M
Bonafè
Department of Experimental, Diagnostic and Specialty Medicine, University Hospital S. Orsola Malpighi, Via Massarenti 9, 40138, Bologna, Italy.
Maria Carla
MC
Re
Center for Applied Biomedical Research - CRBA, University Hospital S.Orsola-Malpighi, Via Massarenti 9, 40138, Bologna, Italy.
Paolo
P
Garagnani
Center for Applied Biomedical Research - CRBA, University Hospital S.Orsola-Malpighi, Via Massarenti 9, 40138, Bologna, Italy.
Vilma
V
Mantovani
D005260:Female; D005838:Genotype; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D027383:Papillomaviridae / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000821:virology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
Cervical cancer; Genotyping; HPV; High-throughput; Inno-LiPA; MALDI-TOF MS