Comparative Immunohistochemistry of Placental Corticotropin-Releasing Hormone and the Transcription Factor RelB-NFκB2 Between Humans and Nonhuman Primates.
The transcription factor RelB-NFκB2, activated by the noncanonical NFκB pathway, positively regulates corticotropin-releasing hormone (CRH) and prostaglandin production in the term human placenta and may play an important role in the timing of human parturition. Here we explored whether RelB-NFκB2 signaling plays a role in parturition in nonhuman anthropoid primates. We performed immunohistochemical staining to assess the correlation between CRH and nuclear activity of RelB-NFκB2 heterodimers in term placentas from humans, 3 catarrhine primate species, and a single platyrrhine primate species. Consistent with our previous studies, the human placenta showed cytoplasmic staining for CRH and nuclear staining for RelB-NFκB2. Similar staining patterns were noted in the 3 catarrhine primates (chimpanzee, baboon, and rhesus macaque). The platyrrhine (marmoset) placentas stained positively for CRH and RelB but not for NFκB2. Catarrhine (but not platyrrhine) nonhuman primate term placentas demonstrate the same CRH staining and nuclear localization patterns of RelB and NFκB2 as does human placenta. These results suggest that catarrhine primates, particularly rhesus macaques, may serve as useful animal models to study the biologic significance of the noncanonical NFκB pathway in human pregnancy.
Department of Obstetrics, Gynecology, and Reproductive Sciences, Rutgers Robert Wood Johnson Medical School, New Brunswick, New Jersey, USA. rosentj@rwjms.rutgers.edu.
Todd
T
Rosen
Research Department, American College of Obstetricians and Gynecologists, Washington, District of Columbia, USA; Department of Neuroscience, Georgetown University, Washington, District of Columbia, USA.
Jay
J
Schulkin
Nutrition Laboratory, Conservation Ecology Center, Smithsonian Conservation Biology Institute, National Zoological Park, Washington, District of Columbia, USA.
Michael
M
Power
Tufts University School of Medicine, Department of Obstetrics and Gynecology, Boston, Massachusetts, USA.
Serkalem
S
Tadesse
Tufts University School of Medicine, Department of Obstetrics and Gynecology, Boston, Massachusetts, USA.
Errol R
ER
Norwitz
Department of Pathology, Saint Peter's University Hospital, New Brunswick, New Jersey, USA.
Zhaoqin
Z
Wen
Department of Obstetrics, Gynecology, and Reproductive Sciences, Rutgers Robert Wood Johnson Medical School, New Brunswick, New Jersey, USA. wangbi@rwjms.rutgers.edu.
Bingbing
B
Wang
D000818:Animals; D002144:Callithrix / Q000378:metabolism; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008253:Macaca mulatta / Q000378:metabolism; D023421:Models, Animal; D052003:NF-kappa B p52 Subunit / Q000378:metabolism*; D002679:Pan troglodytes / Q000378:metabolism; D048529:Papio hamadryas / Q000378:metabolism; D010920:Placenta / Q000378:metabolism*; D010926:Placental Hormones / Q000378:metabolism; D011247:Pregnancy; D011323:Primates / Q000378:metabolism*; D015398:Signal Transduction; D013045:Species Specificity; D051841:Transcription Factor RelB / Q000378:metabolism*
D052003:NF-kappa B p52 Subunit; D010926:Placental Hormones; D051841:Transcription Factor RelB; D003346:Corticotropin-Releasing Hormone