Self-assembly of SiO2 nanoparticles for the potentiometric detection of neurotransmitter acetylcholine and its inhibitor.
The detection and quantification of neurotransmitter acetylcholine (ACh) are relevant because modifications in the ACh levels constitute a threat to human health. The biological regulator of this neurotransmitter is acetylcholinesterase (AChE), an enzyme that catalyzes the hydrolysis of ACh to choline and acetic acid. However, its activity is inhibited in the presence of organophosphate and carbamate pesticides, compromising the degradation of the neurotransmitter. There has been a growing interest in faster and more sensitive detection systems that include new methods and materials for the determination of the ACh concentration. This paper proposes a potentiometric biosensor for the detection of neurotransmitter ACh and its inhibitors, specifically organophosphate pesticide methamidophos. The biosensor is based on a self-assembled platform formed by poly(allylamine) hydrochloride (PAH) and silicon dioxide nanoparticles (SiO2-Np) that contains the immobilized enzyme AChE. First, the responses of the biosensor were investigated for different concentrations of ACh in buffer solutions. After quantifying ACh, the inhibition of AChE in the presence of methamidophos was determined, enabling the quantification of methamidophos expressed as the percentage of enzyme inhibition. The potential advantages of this biosensor include simplicity in building the electrode, possible production on an industrial scale, limited need for qualified personnel to operate the device and low processing cost.
Izabela G
IG
Arruda
Francisco E G
FE
Guimarães
Romildo J
RJ
Ramos
Nirton C S
NC
Vieira
D000109:Acetylcholine / Q000032:analysis* / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000110:Acetylcholinesterase / Q000737:chemistry / Q000378:metabolism; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D007306:Insecticides / Q000032:analysis; D053758:Nanoparticles / Q000737:chemistry*; D009946:Organothiophosphorus Compounds / Q000032:analysis; D012822:Silicon Dioxide / Q000737:chemistry*
D004800:Enzymes, Immobilized; D007306:Insecticides; D009946:Organothiophosphorus Compounds; D012822:Silicon Dioxide; C014655:methamidophos; D000110:Acetylcholinesterase; D000109:Acetylcholine