Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins.
Hydrogen-rich water (HRW) has anti-oxidant activities, and it exerts neuroprotective effects during ischemia-reperfusion brain injury. Parvalbumin and hippocalcin are two calcium buffering proteins, which are involved in neuronal differentiation, maturation and apoptosis. The aim of this study was to investigate whether HRW could moderate parvalbumin and hippocalcin expression during ischemic brain injury and glutamate toxicity-induced neuronal cell death. Focal brain ischemia was induced in male Sprague-Dawley rats by middle cerebral artery occlusion (MCAO). Rats were treated with H2O or HRW (6 ml/kg per rat) before and after MCAO, and cerebral cortical tissues were collected 1, 7 and 14 days after MCAO. Based on our results, HRW treatment was able to reduce brain infarct volume and improve neurological function following ischemic brain injury. In addition, HRW prevented the ischemia-induced reduction of parvalbumin and hippocalcin levels in vivo and also reduced the glutamate toxicity-induced death of neurons, including the dose-dependent reduction of glutamate toxicity-associated proteins in vitro. Moreover, HRW attenuated the glutamate toxicity-induced elevate in intracellular Ca(2+) levels. All these results suggest that HRW could protect against ischemic brain injury and that the maintenance of parvalbumin and hippocalcin levels by HRW during ischemic brain injury might contribute to the neuroprotective effects against neuron damage.
Department of Neurology, Liyuan Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430000, PR China.
Li
L
Han
Department of Neurosurgery, Beijing Tian Tan Hospital, Capital Medical University, Tiantan Xili 6, Dongcheng District, Beijing 100050, PR China.
Runfa
R
Tian
Key Laboratory of Neurological Diseases of Ministry of Education, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430000, PR China.
Huanhuan
H
Yan
Department of Physiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430000, PR China; Department of Pathology and Pathophysiology, School of Basic Medicine, Institute for Brain Research, Huazhong University of Science and Technology, Wuhan 430000, PR China.
Lei
L
Pei
Department of Neurosurgery, Beijing Tian Tan Hospital, Capital Medical University, Tiantan Xili 6, Dongcheng District, Beijing 100050, PR China.
Zonggang
Z
Hou
Department of Neurosurgery, Beijing Tian Tan Hospital, Capital Medical University, Tiantan Xili 6, Dongcheng District, Beijing 100050, PR China.
Shuyu
S
Hao
Department of Biomedical Sciences, Ohio University Heritage College of Osteopathic Medicine, Athens, OH 45701, USA.
Yang V
YV
Li
Department of Pathology and Pathophysiology, School of Basic Medicine, Institute for Brain Research, Huazhong University of Science and Technology, Wuhan 430000, PR China; Department of Pathophysiology, Key Laboratory of Ministry of Education of Neurological Diseases, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430000, PR China.
Qing
Q
Tian
Department of Neurosurgery, Beijing Tian Tan Hospital, Capital Medical University, Tiantan Xili 6, Dongcheng District, Beijing 100050, PR China; Neurotrauma Laboratory, Beijing Neurosurgical Institute, Capital Medical University, Beijing 100050, PR China. Electronic address: liubaiyun1212@163.com.
Baiyun
B
Liu
Department of Neurology, Liyuan Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430000, PR China; Department of Pathology and Pathophysiology, School of Basic Medicine, Institute for Brain Research, Huazhong University of Science and Technology, Wuhan 430000, PR China. Electronic address: zhangqi@mails.tjmu.edu.cn.
Qi
Q
Zhang
D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism* / Q000517:prevention & control / Q000523:psychology; D002118:Calcium / Q000378:metabolism*; D018698:Glutamic Acid / Q000633:toxicity; D051597:Hippocalcin / Q000378:metabolism*; D006859:Hydrogen / Q000008:administration & dosage*; D020244:Infarction, Middle Cerebral Artery / Q000150:complications; D008297:Male; D018696:Neuroprotective Agents / Q000008:administration & dosage*; D010320:Parvalbumins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014867:Water / Q000008:administration & dosage* / Q000737:chemistry
D018696:Neuroprotective Agents; D010320:Parvalbumins; D014867:Water; D051597:Hippocalcin; D018698:Glutamic Acid; D006859:Hydrogen; D002118:Calcium
Hippocalcin; Hydrogen-rich water; Ischemic brain injury; Neuroprotection; Parvalbumin