MicroRNA-19a regulates proliferation and apoptosis of castration-resistant prostate cancer cells by targeting BTG1.
MicroRNAs (miRNAs) play a significant role in tumor development. Recent studies indicate that miRNAs are implicated in prostate cancer (PCa). In this study, we found that miR-19a expression was significantly increased in castration-resistant prostate cancer (CRPC) tissues compared with androgen-dependent prostate cancer (ADPC) tissues. We found that inhibiting the overexpression of miR-19a in CRPC cells suppressed proliferation and increased apoptosis. Additionally, we found that miR-19a repressed BTG1 expression by binding to its 3'-untranslated region. The overexpression of BTG1 in CRPC cells significantly suppressed proliferation and increased apoptosis. We conclude that miR-19a regulates proliferation and apoptosis of CRPC cells by directly targeting the tumor suppressor gene BTG1.
Department of Urology, Affiliated Zhongda Hospital of Southeast University, Nanjing, China. Electronic address: kai.5391@hotmail.com.
Kai
K
Lu
Department of Medicine, Southeast University, Nanjing, China.
Chunhui
C
Liu
Department of Medicine, Southeast University, Nanjing, China.
Tao
T
Tao
Department of Medicine, Southeast University, Nanjing, China.
Xiaowen
X
Zhang
Department of Urology, Affiliated Zhongda Hospital of Southeast University, Nanjing, China.
Lei
L
Zhang
Department of Urology, Affiliated Zhongda Hospital of Southeast University, Nanjing, China; Department of Medicine, Southeast University, Nanjing, China.
Chao
C
Sun
Department of Urology, Affiliated Zhongda Hospital of Southeast University, Nanjing, China.
Yiduo
Y
Wang
Department of Urology, Affiliated Zhongda Hospital of Southeast University, Nanjing, China.
Shuqiu
S
Chen
Department of Urology, Affiliated Zhongda Hospital of Southeast University, Nanjing, China; Department of Medicine, Southeast University, Nanjing, China.
Bin
B
Xu
Department of Urology, Affiliated Zhongda Hospital of Southeast University, Nanjing, China; Department of Medicine, Southeast University, Nanjing, China.
Ming
M
Chen
D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D020320:Oligoribonucleotides, Antisense / Q000235:genetics; D064129:Prostatic Neoplasms, Castration-Resistant / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D047368:Tumor Burden / Q000235:genetics; D023041:Xenograft Model Antitumor Assays
D020413:3' Untranslated Regions; C549214:MIRN19A microRNA, human; D035683:MicroRNAs; D009363:Neoplasm Proteins; D020320:Oligoribonucleotides, Antisense; C074086:BTG1 protein, human
Apoptosis; BTG1; MiR-19a; Proliferation; Prostate cancer