Interaction of novel fluorescent nanoscale ionic silicate platelets with biomaterials for biosensors.
The nano silicate platelets (NSPs) of 100 × 100 × 1 nm(3) in dimension were previously derived from the exfoliation of naturally occurring sodium montmorillonite clay, and their affinity to the surface of bacteria was revealed. The unique characteristics of ionic charges (≡Si-O-Na(+)) and the presence of siloxanol functionalities (≡Si-OH) allowed the organic modification of NSP to form NSP-tethering poly(hydroxyethyl methacrylate) (PHEMA) pendants through a sol-gel and living polymerization. By attaching nathphalimide-type fluorescence onto NSP-PHEMA, a new class of fluorescent organic-inorganic hybrid (NSP-PHEMA-HA), was prepared and its photoluminescence (PL) and bacterial trapping properties were characterized. The investigation of PL emission revealed that the fluorescent NSP hybrids could be used to detect bacteria and possess the potential for the biosensor applications.
†Institute of Polymer Science and Engineering, National Taiwan University, Taipei, 10617, Taiwan.
Hsiao-Chu
HC
Lin
†Institute of Polymer Science and Engineering, National Taiwan University, Taipei, 10617, Taiwan.
Jiang-Jen
JJ
Lin
†Institute of Polymer Science and Engineering, National Taiwan University, Taipei, 10617, Taiwan.|‡Department of Chemical Engineering, National Taiwan University, Taipei, 10617, Taiwan.
Yu-Jane
YJ
Sheng
D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D004926:Escherichia coli / Q000302:isolation & purification*; D005456:Fluorescent Dyes / Q000737:chemistry; D007477:Ions; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry*; D017640:Silicates / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D005456:Fluorescent Dyes; D007477:Ions; D017640:Silicates; D011102:Polyhydroxyethyl Methacrylate
PHEMA; clay; nano silicate platelet; photoluminescence; physical trapping