Overexpression of feline tripartite motif-containing 25 interferes with the late stage of feline leukemia virus replication.
Tripartite motif-containing 25 (TRIM25) regulates various cellular processes through E3 ubiquitin ligase activity. Previous studies have revealed that the expression of TRIM25 is induced by type I interferon and that TRIM25 is involved in the host cellular innate immune response against retroviral infection. Although retroviral infection is prevalent in domestic cats, the roles of feline TRIM25 in the immune response against these viral infections are poorly understood. Because feline TRIM25 is expected to modulate the infection of feline leukemia virus (FeLV), we investigated its effects on early- and late-stage FeLV replication. This study revealed that ectopic expression of feline TRIM25 in HEK293T cells reduced viral protein levels leading to the inhibition of FeLV release. Our findings show that feline TRIM25 has a potent antiviral activity and implicate an antiviral mechanism whereby feline TRIM25 interferes with late-stage FeLV replication.
Laboratory of Veterinary Epizootiology, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, Kameino 1866, Fujisawa, Kanagawa 252-0880, Japan. Electronic address: koba.ryouta@nihon-u.ac.jp.
Ryota
R
Koba
Laboratory of Veterinary Epizootiology, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, Kameino 1866, Fujisawa, Kanagawa 252-0880, Japan.
Keisuke
K
Oguma
Laboratory of Veterinary Epizootiology, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, Kameino 1866, Fujisawa, Kanagawa 252-0880, Japan. Electronic address: sentsui.hiroshi@nihon-u.ac.jp.
Hiroshi
H
Sentsui
D000818:Animals; D000998:Antiviral Agents / Q000276:immunology*; D002371:Cat Diseases / Q000276:immunology*; D002415:Cats; D015870:Gene Expression; D057809:HEK293 Cells; D006801:Humans; D007113:Immunity, Innate; D007370:Interferon Type I / Q000276:immunology*; D005256:Leukemia Virus, Feline / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D012192:Retroviridae Infections / Q000276:immunology / Q000662:veterinary*; D015398:Signal Transduction; D014412:Tumor Virus Infections / Q000276:immunology / Q000662:veterinary*; D044767:Ubiquitin-Protein Ligases / Q000235:genetics* / Q000378:metabolism; D054875:Ubiquitination; D053586:Virus Internalization; D014779:Virus Replication
D000998:Antiviral Agents; D007370:Interferon Type I; D044767:Ubiquitin-Protein Ligases
Antiviral activity; Cat; Feline leukemia virus; Tripartite motif-containing 25 (TRIM25)