The effect of Nesfatin-1 on heart L-type Ca²⁺ channel α1c subunit in rats subjected to chronic restraint stress.
BACKGROUND
Elevated L-type Ca²⁺channel expression level increases Ca²⁺ influx. This can cause hypertrophy and pathological remodeling of the heart especially under stress conditions. Nesfatin-1 can activate hypothalamic L, P and Q type Ca²⁺ channels and increase insulin secretion in pancreatic islet beta cells via activation of L-type Ca²⁺ channels. On the other hand, the effect of nesfatin-1 on cardiac L-type Ca²⁺ channels has not been studied yet.


OBJECTIVE
We aimed to identify the effect of peripheral chronic Nesfatin-1 application on cardiac L-type Ca²⁺ channel α1c subunit expression level in normal rats and those subjected to chronic restraint stress.


METHODS
Three-month aged Wistar albino rats were randomly divided into 4 groups (n = 7) as Control, Stress, Control+Nesfatin-1, and Nesfatin-1+Stress. Rats in groups subjected to restraint stress were placed in a specially built size-manipulable cabin for 2 h/day (between 10:00 and 12:00 a.m.) for 10 consecutive days without allowing water and food intake. Nesfatin-1 segment (0.25 nmol/g bw intraperitoneally) was applied during the 10 consecutive days. Western blot analyses were performed to determine the expression level of L-type Ca²⁺ channel α1c subunit protein in rat cardiac extracts.


RESULTS
Cardiac L-type Ca²⁺ channel α1c subunit protein expression levels were increased significantly after chronic peripheral Nesfatin-1 application in rats subjected to restraint stress (p = 0.032).


CONCLUSIONS
We can conclude that Nesfatin-1 can cause cardiac failures during clinical treatments by elevating cardiac L-type Ca²⁺ channel α1c subunit protein expression level (Fig. 2, Ref. 26).
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Turgut
D000818:Animals; D020746:Calcium Channels, L-Type / Q000187:drug effects* / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000494:pharmacology*; D004268:DNA-Binding Proteins / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects*; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000494:pharmacology*; D000080866:Nucleobindins; D051381:Rats; D017208:Rats, Wistar; D012149:Restraint, Physical; D013315:Stress, Psychological / Q000378:metabolism*
C504728:Cacna1c protein, rat; D020746:Calcium Channels, L-Type; D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; D009419:Nerve Tissue Proteins; C000630652:Nucb1 protein, rat; D000080866:Nucleobindins
L-type Ca2+ channel α1c subunit; Western blot.; cardiac failure; chronic restraint stress; nesfatin-1