Measurement of photosynthesis using PAM technology in a purple sulfur bacterium Thermochromatium tepidum (Chromatiaceae).
We demonstrate that Blue-diode-based pulse amplitude modulation (PAM) technology can be used to measure the photosynthetic electron transport rate (ETR) of purple sulfur bacteria (Thermochromatium tepidum, Chromatiaceae). Previous studies showed that PAM technology could be used to estimate photosynthesis in purple nonsulfur bacteria and so PAM technology can be used to estimate photosynthesis of both kinds of purple photosynthetic bacteria. The absorptance of Thermochromatium films on glass fiber disks was measured and used to calculate actual ETR. ETR vs Irradiance (P vs E) curves fitted the waiting-in-line model (ETR = (ETRmax × E/Eopt) × exp (1−E/Eopt)). Yield (Y) was only ≈ 0.3–0.4. Thermochromatium saturates at 325 ± 13.8 μmol photons m(−2) s(−1) or ≈15% sunlight and shows photoinhibition at high irradiances. A pond of Thermochromatium would exhibit classic surface inhibition. Photosynthesis is extremely low in the absence of an electron source: ETR increases in the presence of acetate (5 mol m(−3)) provided as an organic carbon source and also increases in the presence of sulfite (3 mol m(−3)) but not sulfide and is only marginally increased by the presence of Fe(2+). Nonphotochemical quenching does occur in Thermochromatium but at very low levels compared to oxygenic photo-organisms or Rhodopseudomonads.
Raymond J
RJ
Ritchie
Nutsara
N
Mekjinda
D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000378:metabolism*; D002841:Chromatiaceae / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects*; D004579:Electron Transport / Q000528:radiation effects; D005470:Fluorometry / Q000295:instrumentation / Q000379:methods*; D005898:Glass; D010788:Photosynthesis / Q000502:physiology / Q000528:radiation effects*; D013472:Sunlight
D001426:Bacterial Proteins; C026083:bacteriochlorophyll A proteins, Bacteria; C009753:fiberglass