Isolation and maintenance of Balantidium coli (Malmsteim, 1857) cultured from fecal samples of pigs and non-human primates.
Balantidium coli is a protozoa that can determine dysentery in humans, pigs and non-human primates having zoonotic potential. The lack of standardization in isolation and maintenance hinders the development of research on its biology and epidemiology. This study is aimed to standardize the isolation and maintenance of this parasite from animal feces, in culture medium, Pavlova modified. From 2012 to 2014, 1905 fecal samples were collected from captive animals of Rio de Janeiro. Were selected for isolation samples with a minimum of 10 trophozoites and/or 30 cysts of B. coli, totaling 88 pigs, 26 Cynomolgus and 90 rhesus macaques. In the presence of cysts, the sample was homogenized in saline solution, 500 μL was removed and inoculated into culture medium. The material that contained trophozoites the inoculum was made from 240 μL of fecal solution. All inoculate tubes with the subcultures were kept at 36°C, and sterile rice starch was always added to the medium. The parasites isolate from pigs, 34%, and from Cynomolgus 38.4% were maintained in vitro for a period of more than 24 months. These procedures proved to be adequate for isolation and maintenance of B. coli from different animals, they were found to be inexpensive and easy to perform.
Oswaldo Cruz Foundation (Fiocruz, Rio de Janeiro), Oswaldo Cruz Institute, Toxoplasmosis Laboratory, Avenue Brazil, 4365, Manguinhos, Rio de Janeiro 21045900, Brazil. Electronic address: alynnedsb@gmail.com.
Alynne da Silva
Ada S
Barbosa
Federal Fluminense University, Biomedical Institute, Department of Microbiology and Parasitology, Professor Hernani Mello Street, São Domingos, Niterói, Rio de Janeiro 24210-130, Brazil. Electronic address: otiliobastos@gmail.com.
Otilio Machado Pereira
OM
Bastos
Federal Fluminense University, Biomedical Institute, Department of Microbiology and Parasitology, Professor Hernani Mello Street, São Domingos, Niterói, Rio de Janeiro 24210-130, Brazil. Electronic address: claudiauchoa@vm.uff.br.
Claudia M Antunes
CM
Uchôa
Primate Center of Rio de Janeiro - CPRJ/INEA, RJ, Paradise Road, Guapimirim, Rio de Janeiro 25940-000, Brazil. Electronic address: pissinatticprj@globo.com.
Alcides
A
Pissinatti
Oswaldo Cruz Foundation (Fiocruz, Rio de Janeiro), Building Service Nonhuman primates (CECAL), Oswaldo Cruz Foundation, Building 108, Brazil Avenue, 4365, Manguinhos, Rio de Janeiro 21045900, Brazil. Electronic address: prhricardo@gmail.com.
Paulo Ricardo
PR
Ferreira Filho
Federal Fluminense University, Biomedical Institute, Department of Microbiology and Parasitology, Professor Hernani Mello Street, São Domingos, Niterói, Rio de Janeiro 24210-130, Brazil. Electronic address: lais.dib@hotmail.com.
Lais Verdan
LV
Dib
Federal Fluminense University, Biomedical Institute, Department of Microbiology and Parasitology, Professor Hernani Mello Street, São Domingos, Niterói, Rio de Janeiro 24210-130, Brazil. Electronic address: dudadauna@gmail.com.
Eduarda Peixoto
EP
Azevedo
Federal Fluminense University, Biomedical Institute, Department of Microbiology and Parasitology, Professor Hernani Mello Street, São Domingos, Niterói, Rio de Janeiro 24210-130, Brazil. Electronic address: mayaraperlingeiro@yahoo.com.br.
Mayara Perlingeiro
MP
de Siqueira
Federal Fluminense University, Biomedical Institute, Department of Microbiology and Parasitology, Professor Hernani Mello Street, São Domingos, Niterói, Rio de Janeiro 24210-130, Brazil. Electronic address: matheus_lcardozo@hotmail.com.
Matheus Lessa
ML
Cardozo
Oswaldo Cruz Foundation (Fiocruz, Rio de Janeiro), Oswaldo Cruz Institute, Toxoplasmosis Laboratory, Avenue Brazil, 4365, Manguinhos, Rio de Janeiro 21045900, Brazil. Electronic address: mramendoeira@globo.com.
Maria Regina Reis
MR
Amendoeira
D000820:Animal Diseases / Q000469:parasitology*; D000818:Animals; D001447:Balantidiasis / Q000469:parasitology / Q000662:veterinary*; D001448:Balantidium / Q000166:cytology / Q000254:growth & development / Q000302:isolation & purification*; D001938:Brazil; D003470:Culture Media; D005243:Feces / Q000469:parasitology; D011323:Primates / Q000469:parasitology*; D013552:Swine; D013553:Swine Diseases / Q000469:parasitology*
D003470:Culture Media
Balantidium coli; Brazil; Isolation; Non-human primates; Pig; Xenic cultivation