Polyethylene Oxide (PEO) and Polyethylene Glycol (PEG) Polymer Sieving Matrix for RNA Capillary Electrophoresis.
The selection of sieving polymer for RNA fragments separation by capillary electrophoresis is imperative. We investigated the separation of RNA fragments ranged from 100 to 10,000 nt in polyethylene glycol (PEG) and polyethylene oxide (PEO) solutions with different molecular weight and different concentration. We found that the separation performance of the small RNA fragments (<1000 nt) was improved with the increase of polymer concentration, whereas the separation performance for the large ones (>4000 nt) deteriorated in PEG/PEO solutions when the concentration was above 1.0%/0.6%, respectively. By double logarithmic plot of mobility and RNA fragment size, we revealed three migration regimes for RNA in PEG (300-500k) and PEO (4,000k). Moreover, we calculated the smallest resolvable nucleotide length (Nmin) from the resolution length analysis.
Institute of Photonics and Bio-medicine (IPBM), Graduate School of Science, East China University of Science and Technology (ECUST), 130 Meilong Road, Shanghai 200237, China. Engineering Research Center of Optical Instrument and System, Ministry of Education, Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology, No. 516 Jungong Road, Shanghai 200093, China; Department of Applied Physics, Graduate School of Engineering, Osaka University, Yamadaoka, Suita-city, Osaka, 565-0871, Japan.
Yoshinori
Y
Yamaguchi
Institute of Photonics and Bio-medicine (IPBM), Graduate School of Science, East China University of Science and Technology (ECUST), 130 Meilong Road, Shanghai 200237, China.
Zhenqing
Z
Li
College of Photoelectric Engineering, Changzhou Institute of Technology, No.299, Tongjiangnan Road, Changzhou, 213002, China.
Xifang
X
Zhu
Institute of Photonics and Bio-medicine (IPBM), Graduate School of Science, East China University of Science and Technology (ECUST), 130 Meilong Road, Shanghai 200237, China.
Chenchen
C
Liu
Institute of Photonics and Bio-medicine (IPBM), Graduate School of Science, East China University of Science and Technology (ECUST), 130 Meilong Road, Shanghai 200237, China.
Dawei
D
Zhang
Engineering Research Center of Optical Instrument and System, Ministry of Education, Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology, No. 516 Jungong Road, Shanghai 200093, China.
Xiaoming
X
Dou
D019075:Electrophoresis, Capillary / Q000379:methods*; D011092:Polyethylene Glycols / Q000737:chemistry*; D012313:RNA / Q000302:isolation & purification*; D012996:Solutions
D012996:Solutions; D011092:Polyethylene Glycols; D012313:RNA