Highly sensitive detection for proteins using graphene oxide-aptamer based sensors.
In recent years, the detection of proteins by using bare graphene oxide (GO) to quench the fluorescence of fluorescein-labeled aptamers has been reported. However, the proteins can be adsorbed on the surface of bare GO to prevent the sensitivity from further being improved. In order to solve this problem, polyethylene glycol (PEG)-protected GO was used to prevent the proteins using thrombin as an example from nonspecific binding. The detection limit was improved compared to bare GO under the optimized ratio of GO to PEG concentration. The results show that our method is a promising technique for the detection of proteins.
Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, P. R. China. gaoli@ujs.edu.cn.
Li
L
Gao
Qin
Q
Li
Raoqi
R
Li
Lirong
L
Yan
Yang
Y
Zhou
Keping
K
Chen
Haixia
H
Shi
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D010087:Oxides / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D011506:Proteins / Q000032:analysis*; D015203:Reproducibility of Results; D013917:Thrombin / Q000032:analysis
D052157:Aptamers, Nucleotide; D010087:Oxides; D011506:Proteins; D011092:Polyethylene Glycols; D006108:Graphite; D013917:Thrombin