Identification of subgingival periodontal pathogens and association with the severity of periodontitis in patients with chronic kidney diseases: a cross-sectional study.
BACKGROUND
The aim of our study was to assess the subgingival profile of 9 periodontal pathogens, by means of real-time PCR, in a group of predialysis chronic kidney disease patients with and without periodontal disease and to identify the risk factors associated with periodontal disease in these patients.


METHODS
This is a single centre cross-sectional cohort study performed on 70 CKD patients. Patients received a full-mouth periodontal examination and the following parameters were assessed: periodontal pocket depth (PPD), clinical attachment level, bleeding on probing, and plaque index; subgingival biofilm samples were collected from the deepest periodontal pocket of each quadrant and were pooled in one transporting unit. Clinical data were drawn from the medical file of the patients.


RESULTS
T. denticola (P = 0.001), T. forsythia (P < 0.001), and P. micros (P = 0.003) are significantly associated with periodontal disease in CKD subjects but in a multivariate model only age and T. forsythia remain independent risk factors for periodontal disease in patients with CKD.


CONCLUSIONS
In our cohort, age and T. forsythia are independently associated with periodontitis in CKD patients. Within the limits of this study, CKD was not significantly associated with a particular subgingival periodontal pathogens profile in periodontitis patients.
Department of Periodontology, "Carol Davila" University of Medicine and Pharmacy, Dionisie Lupu Street No. 37, District 1, 020021 Bucharest, Romania.
Fidan Bahtiar
0000-0001-5402-9253
FB
Ismail
Center of Internal Medicine-Nephrology, Fundeni Clinical Institute, 258 Fundeni Street, District 2, 022328 Bucharest, Romania.
Gener
0000-0003-3306-189X
G
Ismail
Department of Periodontology, "Carol Davila" University of Medicine and Pharmacy, Dionisie Lupu Street No. 37, District 1, 020021 Bucharest, Romania.
Anca Silvia
AS
Dumitriu
Center for Uronephrology and Renal Transplantation, Fundeni Clinical Institute, 258 Fundeni Street, District 2, 022328 Bucharest, Romania.
Catalin
C
Baston
Center of Internal Medicine-Nephrology, Fundeni Clinical Institute, 258 Fundeni Street, District 2, 022328 Bucharest, Romania.
Vlad
V
Berbecar
Center of Internal Medicine-Nephrology, Fundeni Clinical Institute, 258 Fundeni Street, District 2, 022328 Bucharest, Romania.
Roxana
R
Jurubita
Center of Internal Medicine-Nephrology, Fundeni Clinical Institute, 258 Fundeni Street, District 2, 022328 Bucharest, Romania.
Andreea
A
Andronesi
Department of Periodontology, "Carol Davila" University of Medicine and Pharmacy, Dionisie Lupu Street No. 37, District 1, 020021 Bucharest, Romania.
Horia Traian
0000-0002-1799-668X
HT
Dumitriu
Center for Uronephrology and Renal Transplantation, Fundeni Clinical Institute, 258 Fundeni Street, District 2, 022328 Bucharest, Romania.
Ioanel
0000-0002-3162-1366
I
Sinescu
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D003430:Cross-Sectional Studies; D003774:Dental Plaque Index; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010514:Periodontal Pocket / Q000382:microbiology* / Q000473:pathology; D010518:Periodontitis / Q000150:complications / Q000382:microbiology* / Q000473:pathology; D016966:Porphyromonas gingivalis / Q000302:isolation & purification / Q000472:pathogenicity; D051436:Renal Insufficiency, Chronic / Q000150:complications* / Q000382:microbiology / Q000473:pathology; D048373:Treponema denticola / Q000302:isolation & purification / Q000472:pathogenicity