Precise Three-Dimensional Scan-Free Multiple-Particle Tracking over Large Axial Ranges with Tetrapod Point Spread Functions.
We employ a novel framework for information-optimal microscopy to design a family of point spread functions (PSFs), the Tetrapod PSFs, which enable high-precision localization of nanoscale emitters in three dimensions over customizable axial (z) ranges of up to 20 μm with a high numerical aperture objective lens. To illustrate, we perform flow profiling in a microfluidic channel and show scan-free tracking of single quantum-dot-labeled phospholipid molecules on the surface of living, thick mammalian cells.
†Department of Chemistry, Stanford University, 375 North-South Mall, Stanford, California 94305, United States.
Yoav
Y
Shechtman
†Department of Chemistry, Stanford University, 375 North-South Mall, Stanford, California 94305, United States.
Lucien E
LE
Weiss
‡Institute for Computational and Mathematical Engineering, 475 Via Ortega, Stanford, California 94305, United States.
Adam S
AS
Backer
†Department of Chemistry, Stanford University, 375 North-South Mall, Stanford, California 94305, United States.
Steffen J
SJ
Sahl
†Department of Chemistry, Stanford University, 375 North-South Mall, Stanford, California 94305, United States.
W E
WE
Moerner
D002462:Cell Membrane / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D056656:Lab-On-A-Chip Devices; D008563:Membrane Lipids / Q000737:chemistry*; D008853:Microscopy / Q000379:methods*; D045663:Quantum Dots / Q000737:chemistry*
D008563:Membrane Lipids
3D imaging; PSF engineering; nanoscopy; single particle tracking; single-molecule imaging; super-resolution microscopy