Biomarker Characterization by MALDI-TOF/MS.
Mass spectrometric techniques frequently used in clinical diagnosis, such as gas chromatography-mass spectrometry, liquid chromatography-mass spectrometry, ambient ionization mass spectrometry, and matrix-assisted laser desorption ionization/time-of-flight mass spectrometry (MALDI-TOF/MS), are discussed. Due to its ability to rapidly detect large biomolecules in trace amounts, MALDI-TOF/MS is an ideal tool for characterizing disease biomarkers in biologic samples. Clinical applications of MS for the identification and characterization of microorganisms, DNA fragments, tissues, and biofluids are introduced. Approaches for using MALDI-TOF/MS to detect various disease biomarkers including peptides, proteins, and lipids in biological fluids are further discussed. Finally, various sample pretreatment methods which improve the detection efficiency of disease biomarkers are introduced.
Department of Cosmetic Applications and Management, Yuh-Ing Junior College of Health Care & Management, Kaohsiung, Taiwan.
Yi-Tzu
YT
Cho
Department of Chemistry, National Sun Yat-sen University, Kaohsiung, Taiwan.
Hung
H
Su
Department of Urology, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.
Wen-Jeng
WJ
Wu
Division of Gastroenterology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan; Center for Stem Cell Research, Kaohsiung Medical University, Kaohsiung, Taiwan.
Deng-Chyang
DC
Wu
Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan; Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.
Ming-Feng
MF
Hou
Division of Gastroenterology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan; Center for Stem Cell Research, Kaohsiung Medical University, Kaohsiung, Taiwan.
Chao-Hung
CH
Kuo
Department of Chemistry, National Sun Yat-sen University, Kaohsiung, Taiwan; Center for Stem Cell Research, Kaohsiung Medical University, Kaohsiung, Taiwan; Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan; Department of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung, Taiwan. Electronic address: jetea@mail.nsysu.edu.tw.
Jentaie
J
Shiea
D015415:Biomarkers / Q000032:analysis*; D002624:Chemistry, Clinical / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D059625:Liquid-Liquid Extraction; D049329:Nanostructures; D020641:Polymorphism, Single Nucleotide; D052616:Solid Phase Extraction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D015415:Biomarkers
Biofluids; Biomarkers; Clinical diagnosis; Immunoaffinity; Mass spectrometry; Matrix-assisted laser desorption ionization/time-of-flight mass spectrometry; Multivariate statistics; Nanomaterial