[Gender-dependent expression of ERalpha in arsenic exposed mice offspring's lung tissue].
OBJECTIVE
To observe theexpression variations of estrogen receptor alpha (ERalpha) in different periods of mice offspring and the gender-dependent differences in the lung tissue with parental arsenic exposure.


METHODS
Parental female mice were exposed to arsenic by gavage from gestational day 8th to offspring infancy, and offspring were exposed to arsenic by drinking water after infancy. The expression level of ERalpha mRNA and protein in lung tissue of the male and female offspring in different developmental periods and different doses (low, middle, high) of sodium arsenite exposure were detected by real-time PCR and Western blot.


RESULTS
ERalpha mRNA expression in female lung tissue was lower than male in embryonic period (P<0.05); ERalpha mRNA expression in female lung tissue was higher than that of male in infant and adult periods (middle dose of infancy P<0.05, middle and high doses of adulthood P<0.05); No statistical significances were observed in embryo, infancy and adulthood control groups. ERalpha mRNA expression in female lung tissue of infancy and adulthood was higher than that in embryonic period (low, middle and high dose groups P<0.05). ERa protein expression in arsenic exposed female lung tissue was higher than that of male in infant and adult periods, it was also increased by compared with corresponding control groups (P<0.05). The expression level of ERalpha protein in exposed adult female and male offspring were higher than that of infancy.


CONCLUSIONS
Arsenic infected during pregnancy can increase the lung tissue's ERalpha expression level of female offspring in infancy and adulthood. This result is significant to elucidate the role of environment pollutants in gender difference of lung cancer's occurrence.
Meng-meng
MM
Shi
Guo-jian
GJ
Chen
Yang
Y
Zhang
Wang-jun
WJ
Che
Hao
H
Zhang
D000818:Animals; D001151:Arsenic / Q000633:toxicity*; D018053:Arsenites / Q000633:toxicity; D047628:Estrogen Receptor alpha / Q000378:metabolism*; D005260:Female; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D018811:Maternal Exposure; D051379:Mice; D011247:Pregnancy; D012333:RNA, Messenger; D012737:Sex Factors; D017670:Sodium Compounds / Q000633:toxicity
D018053:Arsenites; D047628:Estrogen Receptor alpha; D012333:RNA, Messenger; D017670:Sodium Compounds; C017947:sodium arsenite; D001151:Arsenic