Miniaturization of a micro-optics array for highly sensitive and parallel detection on an injection moulded lab-on-a-chip.
A miniaturised array of supercritical angle fluorescence (SAF) micro-optics embedded in a microfluidic chamber was fabricated by injection moulding. The fabricated chip could enhance the fluorescence signal around 46 times compared to a conventional microscope. Collection of the fluorescence signal from the SAF array is almost independent of the numerical aperture, and the limit of detection was improved 36-fold using a simple and inexpensive optical detection system.
DTU Nanotech, Department of Micro- and Nanotechnology, Technical University of Denmark, Ørsteds Plads, DK-2800 Kgs. Lyngby, Denmark. anders.wolff@nanotech.dtu.dk.
Tran Quang
TQ
Hung
Yi
Y
Sun
Carl Esben
CE
Poulsen
Than
T
Linh-Quyen
Wai Hoe
WH
Chin
Dang Duong
DD
Bang
Anders
A
Wolff
D004247:DNA; D015342:DNA Probes; D004867:Equipment Design; D057230:Limit of Detection; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D008904:Miniaturization / Q000295:instrumentation*; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods
D015342:DNA Probes; D004247:DNA