The effects of red ginseng saponin fraction-A (RGSF-A) on phagocytosis and intracellular signaling in Brucella abortus infected RAW 264.7 cells.
This study indicated that RGSF-A caused a marked reduction in the adherence, internalization and intracellular growth of Brucella abortus in RGSF-A-treated cells. Furthermore, a decline in the intensity of F-actin fluorescence was observed in RGSF-A-treated cells compared with untreated B. abortus-infected cells. In addition, an evaluation of phagocytic signaling proteins by Western blot analysis revealed an apparent reduction of ERK and p38α phosphorylation levels in B. abortus-infected RGSF-A-treated cells compared with the control. Upon intracellular trafficking of the pathogen, a higher number of B. abortus-containing phagosomes colocalized with LAMP-1 in RGSF-A-treated cells compared with control cells. These results strongly suggest that inhibition of B. abortus uptake could be mediated by suppression in the activation of MAPKs signaling proteins phospho-ERK 1/2, and p38 levels. On the other hand, inhibition of intracellular replication results from the enhancement of phagolysosome fusion in host macrophages. This study highlights the phagocytic and intracellular modulating effect of RGSF-A and its potential as an alternative remedy to control B. abortus infection.
Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Lauren Togonon
LT
Arayan
Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Hannah Leah
HL
Simborio
Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Alisha Wehdnesday Bernardo
AW
Reyes
Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Huynh Tan
HT
Hop
Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea.
WonGi
W
Min
Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Hu Jang
HJ
Lee
College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Republic of Korea.
Man Hee
MH
Rhee
Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Hong Hee
HH
Chang
Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea kimsuk@gnu.ac.kr.
Suk
S
Kim
D000199:Actins / Q000502:physiology; D000818:Animals; D015153:Blotting, Western; D002003:Brucella abortus / Q000187:drug effects* / Q000502:physiology* / Q000648:ultrastructure; D051907:Lysosomal Membrane Proteins / Q000378:metabolism; D008264:Macrophages / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D051379:Mice; D005894:Panax / Q000737:chemistry*; D010587:Phagocytosis / Q000187:drug effects* / Q000502:physiology; D010588:Phagosomes / Q000187:drug effects; D000067996:RAW 264.7 Cells; D012503:Saponins / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects*; D052118:Lysosomal-Associated Membrane Protein 1
D000199:Actins; D051907:Lysosomal Membrane Proteins; D012503:Saponins; C052255:Lamp1 protein, mouse; D052118:Lysosomal-Associated Membrane Protein 1
Brucella abortus; macrophage; phagocytosis; red ginseng saponin fraction-A