Simultaneous determination of plant hormones in peach based on dispersive liquid-liquid microextraction coupled with liquid chromatography-ion trap mass spectrometry.
Fruit development is influenced greatly by endogenous hormones including salicylic acid (SA) and abscisic acid (ABA). Mass spectrometry with high sensitivity has become a routine technology to analyze hormones. However, pretreatment of plant samples remains a difficult problem. Thus, dispersive liquid-liquid microextraction (DLLME) was used to concentrate trace plant hormones before liquid chromatography-ion trap mass spectrometry (LC-ITMS) analysis. Standard curves were linear within the ranges of 0.5-50, 0.2-20ng/mL for SA and ABA, respectively. The correlation coefficients were greater than 0.9995 with recoveries above 87.5%. The limits of detection were 0.2ng/mL for SA and 0.1ng/mL for ABA in spiked water solution, respectively (injection 20μL). The successful analysis of SA and ABA in fruit samples indicated our DLLME-LC-ITMS approach was efficient, allowing reliable quantification of both two compounds from very small amounts of plant material. Moreover, this research revealed the relationship between SA and ABA content and development of peach fruit at different growth stages.
Analytical and Testing Center, Fuzhou University, Fuzhou, Fujian, 350002, China; Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, 350002, Fujian, China.
Qiaomei
Q
Lu
Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, 350002, Fujian, China.
Wenmin
W
Zhang
Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, 350002, Fujian, China.
Jia
J
Gao
Analytical and Testing Center, Fuzhou University, Fuzhou, Fujian, 350002, China; Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, 350002, Fujian, China.
Minghua
M
Lu
Analytical and Testing Center, Fuzhou University, Fuzhou, Fujian, 350002, China; Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, 350002, Fujian, China. Electronic address: zlan@fzu.edu.cn.
Lan
L
Zhang
Food Safety Key Laboratory of Zhejiang Province, College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, Zhejiang, 310035, China; Food Safety Key Laboratory of Liaoning Province, Bohai University, Jinzhou, Liaoning, 121013, China. Electronic address: lijr6491@yahoo.com.cn.
Jianrong
J
Li
D000040:Abscisic Acid / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057230:Limit of Detection; D016014:Linear Models; D059627:Liquid Phase Microextraction / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D010937:Plant Growth Regulators / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D000068239:Prunus persica / Q000737:chemistry*; D015203:Reproducibility of Results; D020156:Salicylic Acid / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification
D010937:Plant Growth Regulators; D000040:Abscisic Acid; D020156:Salicylic Acid
Abscisic acid; Dispersive liquid–liquid microextraction; High performance liquid chromatography; Mass spectrometry; Plant hormone; Salicylic acid