Preliminary evaluation of β3-adrenoceptor agonist-induced 18F-FDG metabolic activity of brown adipose tissue in obese Zucker rat.
BACKGROUND
We have investigated β3-adrenoceptor agonist mediated brown adipose tissue (BAT) activation using (18)F-FDG PET/CT in Zucker lean (ZL) and obese (ZF) rats.


METHODS
(18)F-FDG was injected into ZL and ZF rats pretreated with saline or agonist CL316,243 for scans. (18)F-FDG metabolic activity was computed as standard uptake values.


RESULTS
CL316,243 in ZL activated BAT up to 4-fold compared to saline, while ZF BAT was only up by 2 fold. The decreased activation was consistent with lower β3-adrenoceptor levels in ZF rats.


CONCLUSIONS
The genetically modified ZL and ZF rats may provide a useful rat model to evaluate the significance of β3-adrenoceptor agonist-induced BAT activation in obesity.
Preclinical Imaging, Department of Radiological Sciences, University of California-Irvine, Irvine, CA 92697.
Kimberly N
KN
Schade
Preclinical Imaging, Department of Radiological Sciences, University of California-Irvine, Irvine, CA 92697.
Aparna
A
Baranwal
Preclinical Imaging, Department of Radiological Sciences, University of California-Irvine, Irvine, CA 92697.
Christopher
C
Liang
Preclinical Imaging, Department of Radiological Sciences, University of California-Irvine, Irvine, CA 92697.
M Reza
MR
Mirbolooki
Preclinical Imaging, Department of Radiological Sciences, University of California-Irvine, Irvine, CA 92697. Electronic address: j.mukherjee@uci.edu.
Jogeshwar
J
Mukherjee
D002001:Adipose Tissue, Brown / Q000000981:diagnostic imaging* / Q000378:metabolism*; D058667:Adrenergic beta-3 Receptor Agonists / Q000494:pharmacology*; D000818:Animals; D004149:Dioxoles / Q000494:pharmacology*; D005462:Fluorine Radioisotopes / Q000493:pharmacokinetics; D019788:Fluorodeoxyglucose F18 / Q000493:pharmacokinetics*; D007553:Isotope Labeling; D008297:Male; D008657:Metabolic Clearance Rate; D009765:Obesity / Q000235:genetics; D049268:Positron-Emission Tomography / Q000379:methods*; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics*; D051381:Rats; D011924:Rats, Zucker; D022702:Receptors, Adrenergic, beta-3 / Q000378:metabolism; D013851:Thinness / Q000235:genetics; D014018:Tissue Distribution
D058667:Adrenergic beta-3 Receptor Agonists; D004149:Dioxoles; D005462:Fluorine Radioisotopes; D019275:Radiopharmaceuticals; D022702:Receptors, Adrenergic, beta-3; D019788:Fluorodeoxyglucose F18; C076126:disodium (R,R)-5-(2-((2-(3-chlorophenyl)-2-hydroxyethyl)-amino)propyl)-1,3-benzodioxole-2,3-dicarboxylate
(18)F-FDG; CL316,243; Obesity; PET/CT; β3-Adrenoceptor agonist