Pharmacological postconditioning with lactic acid and hydrogen rich saline alleviates myocardial reperfusion injury in rats.
This study investigated whether pharmacological postconditioning with lactic acid and hydrogen rich saline can provide benefits similar to that of mechanical postconditioning. To our knowledge, this is the first therapeutic study to investigate the co-administration of lactic acid and hydrogen. SD rats were randomly divided into 6 groups: Sham, R/I, M-Post, Lac, Hyd, and Lac + Hyd. The left coronary artery was occluded for 45 min. Blood was withdrawn from the right atrium to measure pH. The rats were sacrificed at different time points to measure mitochondrial absorbance, infarct size, serum markers and apoptotic index. Rats in Lac + Hyd group had similar blood pH and ROS levels when compared to the M-Post group. Additionally, the infarct area was reduced to the same extent in Lac + Hyd and M-Post groups with a similar trends observed for serum markers of myocardial injury and apoptotic index. Although the level of P-ERK in Lac + Hyd group was lower, P-p38/JNK, TNFα, Caspase-8, mitochondrial absorbance and Cyt-c were all similar in Lac + Hyd and M-Post groups. The Lac and Hyd groups were able to partially mimic this protective role. These data suggested that pharmacological postconditioning with lactic acid and hydrogen rich saline nearly replicates the benefits of mechanical postconditioning.
Department of Cardiology, the General Hospital of Jinan Military Command, Jinan 250031, China.
Guoming
G
Zhang
The Center of Cardiovascular Biology and Atherosclerosis Research, University of Texas Medical School at Houston, Houston, TX 77030, USA.
Song
S
Gao
Department of Cardiology, the General Hospital of Jinan Military Command, Jinan 250031, China.
Xiaoyan
X
Li
The Center of Cardiovascular Biology and Atherosclerosis Research, University of Texas Medical School at Houston, Houston, TX 77030, USA.
Lulu
L
Zhang
Department of Cardiology, the General Hospital of Jinan Military Command, Jinan 250031, China.
Hong
H
Tan
Department of Cardiology, the General Hospital of Jinan Military Command, Jinan 250031, China.
Lin
L
Xu
Department of Hematology, School of Pharmacology, Nanjing Medical University, Nanjing, 210029, China.
Yaoyu
Y
Chen
The Center of Cardiovascular Biology and Atherosclerosis Research, University of Texas Medical School at Houston, Houston, TX 77030, USA.
Yongjian
Y
Geng
Department of Center Laboratory, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
Yanliang
Y
Lin
The Center of Cardiovascular Biology and Atherosclerosis Research, University of Texas Medical School at Houston, Houston, TX 77030, USA.
Benjamin
B
Aertker
Department of Ultrasound, the General Hospital of Jinan Military Command, Jinan 250031, China.
Yuanyuan
Y
Sun
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D053181:Caspase 8 / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D006859:Hydrogen / Q000494:pharmacology*; D057775:Ischemic Postconditioning / Q000379:methods; D019344:Lactic Acid / Q000494:pharmacology*; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
D017382:Reactive Oxygen Species; C439524:TNF protein, human; D014409:Tumor Necrosis Factor-alpha; D019344:Lactic Acid; D012965:Sodium Chloride; D006859:Hydrogen; D048051:p38 Mitogen-Activated Protein Kinases; D053181:Caspase 8