Cell cycle and apoptosis regulatory proteins, proliferative markers, cell signaling molecules, CD209, and decorin immunoreactivity in low-grade myxofibrosarcoma and myxoma.
The histologic differential diagnosis between intramuscular myxoma and low-grade myxofibrosarcoma can be quite difficult in some cases. To identify a diagnostic immunohistochemical marker, we compared the staining profiles of 19 different antigens, including cell cycle proteins, apoptosis proteins, and proliferative markers, and selected other signaling and structural proteins in these two tumors. Ten cases each of intramuscular myxoma and low-grade myxofibrosarcoma were stained with antibodies directed against apoptosis regulatory proteins (Bcl2, activated caspase-3, phospho-H2A.X, and cleaved PARP), cell cycle regulatory proteins (Rb1, Cyclin-A, CDKN1B, and Cdt1), proliferative markers (KI67, MCM2, phospho-histone H3, and geminin), cell signalling molecules (c-Myc, EGF, EGFR, PLA2G4A, and HSP90), a dendritic cell marker (CD209), and the extracellular matrix proteoglycan decorin. Staining patterns of myxoma and myxofibrosarcoma were compared using Fisher's exact test and the Mann-Whitney test. For each potential diagnostic marker studied, the proportions of cases scored as positive on both dichotomous or ordinal scales were not significantly different between myxoma and myxofibrosarcoma. Myxoma and myxofibrosarcoma share a common immunophenotype for each of the markers studied. Distinction between these tumors is still predominantly based on morphologic criteria.
Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Medical Center North, CC-3322 1161 21st Ave. South, Nashville, TN, 37232, USA, justin.m.cates@vanderbilt.edu.
Justin M M
JM
Cates
Vincent A
VA
Memoli
Raul S
RS
Gonzalez
D000328:Adult; D000368:Aged; D051017:Apoptosis Regulatory Proteins / Q000032:analysis / Q000096:biosynthesis; D014408:Biomarkers, Tumor / Q000032:analysis*; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000096:biosynthesis; D018797:Cell Cycle Proteins / Q000032:analysis / Q000096:biosynthesis; D058575:Decorin / Q000032:analysis / Q000096:biosynthesis; D003937:Diagnosis, Differential; D005260:Female; D005354:Fibrosarcoma / Q000175:diagnosis*; D006801:Humans; D007150:Immunohistochemistry; D037181:Lectins, C-Type / Q000032:analysis / Q000096:biosynthesis; D008297:Male; D008875:Middle Aged; D009232:Myxoma / Q000175:diagnosis*; D060787:Neoplasm Grading; D011956:Receptors, Cell Surface / Q000032:analysis / Q000096:biosynthesis; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
D051017:Apoptosis Regulatory Proteins; D014408:Biomarkers, Tumor; D015815:Cell Adhesion Molecules; D018797:Cell Cycle Proteins; D058575:Decorin; D037181:Lectins, C-Type; D011956:Receptors, Cell Surface; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin; C546878:DCN protein, human