Heterogeneous Nuclear Ribonucleoprotein A2/B1 as a Target Antigen in Han Chinese for BD Patients.
Behcet's disease (BD) is a recurrent pathema with a typical symptom of inflammation involved in many organs. Previous report indicated that the serum of Korean patients with BD stimulates membrane expression of hnRNP A2/B1 in endothelial cells. In this study, the target 35 kDa recombinant human hnRNP A2/B1 were over-expressed and purified, then sequenced with MALDI-TOF- TOF mass spectrometry. Western blotting and ELISA were applied to detect serum reactivity against hnRNP A2/B1 respectively. The results demonstrate that hnRNP A2/B1 is an autoantigen of BD in Han Chinese population.
Jinghui
J
Liang
Weikang
W
Yang
Xiangyu
X
Meng
Peng
P
Chen
School of Chemistry and Biological Engineering, University of Science and Technology Beijing, 112 Lab, 30 Xueyuan Rd, Beijing 100083, China. hongwudu@ustb.edu.cn.
Hongwu
H
Du
D044466:Asian People; D001323:Autoantibodies / Q000097:blood*; D001324:Autoantigens / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D001528:Behcet Syndrome / Q000276:immunology*; D002681:China; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism
D001323:Autoantibodies; D001324:Autoantigens; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B; D011994:Recombinant Proteins