A study on the effect of cimetidine and L-carnitine on myoglobinuric acute kidney injury in male rats.
Myoglobinuric acute renal failure is the most important life threatening complication of rhabdomyolysis. Iron, free radicals, nitric oxide and cytochrome p450 are involved in the pathogenesis of mARF. The aim of this study is to compare the effect of cimetidine, l-carnitine and both agents together on mARF in rats. Forty rats were divided into 5 groups; group I: control rats, group II: myoglobinuric ARF rats, group III: mARF rats received l-carnitine (200mg/kg, i.p.), group IV: mARF rats received cimetidine (150mg/kg i.p.) and group V: mARF rats received both agents together. 48h after glycerol injection, systolic blood pressure was measured. Urine and blood samples were collected to evaluate urine volume, GFR, BUN, creatinine, K, Na, serum creatine kinase, NO and glutathione levels. Kidney specimens were taken to investigate renal cytochrome p450 and for histological examinations. Cimetidine treatment significantly decreased creatinine, BUN, K, Na, SBP and creatine kinase and increased GFR and urine volume compared to group II. l-carnitine exerted similar changes except for the effect on K and GFR. NO was significantly decreased, while renal glutathione and cytochrome p450 were significantly increased in groups treated with l-carnitine or cimetidine as compared to group II. Combined treatment further improved renal functions, creatine kinase, oxidative stress parameters and SBP as compared to each therapy alone. The histological changes confirmed the biochemical findings. Cimetidine and l-carnitine have protective effects - almost equally - against mARF. Using both agents together, minimises the renal injury.
Physiology Department, Faculty of Medicine, Cairo University, Giza, Egypt. Electronic address: sestaphan@kasralainy.edu.eg.
Suzanne
S
Estaphan
Physiology Department, Faculty of Medicine, Cairo University, Giza, Egypt. Electronic address: profhassan50@yahoo.com.
Hassan
H
Eissa
Physiology Department, Faculty of Medicine, Cairo University, Giza, Egypt. Electronic address: omarattar1993@yahoo.com.
Samah
S
Elattar
Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Giza, Egypt. Electronic address: lailaahmedrashed@gmail.com.
Laila
L
Rashed
Histology Department, Faculty of Medicine, Cairo University, Giza, Egypt. Electronic address: mira_farouk2@yahoo.com.
Mira
M
Farouk
D058186:Acute Kidney Injury / Q000473:pathology*; D000818:Animals; D002331:Carnitine / Q000494:pharmacology*; D002927:Cimetidine / Q000494:pharmacology*; D003402:Creatine Kinase / Q000378:metabolism; D003404:Creatinine / Q000378:metabolism; D004195:Disease Models, Animal; D007668:Kidney / Q000473:pathology*; D008297:Male; D009212:Myoglobinuria / Q000473:pathology*; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D012206:Rhabdomyolysis / Q000473:pathology*
D002927:Cimetidine; D003404:Creatinine; D003402:Creatine Kinase; D002331:Carnitine
Acute kidney injury; Catalytic iron; Cimetidine; Cytochrome P450; Oxidative stress; Rhabdomyolysis; l-Carnitine