Susceptibility of domestic animals to a pseudotype virus bearing RD-114 virus envelope protein.
Retroviral vectors are used for gene transduction into cells and have been applied to gene therapy. Retroviral vectors using envelope protein (Env) of RD-114 virus, a feline endogenous retrovirus, have been used for gene transduction. In this study, we investigated the susceptibility to RD-114 Env-pseudotyped virus in twelve domestic animals including cattle, sheep, horse, pig, dog, cat, ferret, mink, rabbit, rat, mouse, and quail. Comparison of nucleotide sequences of ASCT2 (SLC1A5), a receptor of RD-114 virus, in 10 mammalian and 2 avian species revealed that insertion and deletion events at the region C of ASCT2 where RD-114 viral Env interacts occurred independently in the mouse and rat lineage and in the chicken and quail lineage. By the pseudotype virus infection assay, we found that RD-114 Env-pseudotyped virus could efficiently infect all cell lines except those from mouse and rat. Furthermore, we confirmed that bovine ASCT2 (bASCT2) functions as a receptor for RD-114 virus infection. We also investigated bASCT2 mRNA expression in cattle tissues and found that it is expressed in various tissues including lung, spleen and kidney. These results indicate that retrovirus vectors with RD-114 virus Env can be used for gene therapy in large domestic animals in addition to companion animals such as cat and dog.
Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto 606-8507, Japan; Laboratory of Virolution, Experimental Research Center for Infectious Diseases, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto 606-8507, Japan.
Rie Nakaoka
RN
Miyaho
Department of Molecular Life Science, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa 259-1193, Japan; Micro/Nano Technology Center, Tokai University, 411 Kitakaname, Hiratsuka, Kanagawa 259-1292, Japan. Electronic address: so@tokai.ac.jp.
So
S
Nakagawa
Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto 606-8507, Japan; Laboratory of Virolution, Experimental Research Center for Infectious Diseases, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto 606-8507, Japan.
Akira
A
Hashimoto-Gotoh
Department of Infectious Diseases, Kyoto Prefectural University of Medicine, Kawaramachi-hirokoji, Kamigyo-ku, Kyoto 606-8566, Japan.
Yuki
Y
Nakaya
Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto 606-8507, Japan; Laboratory of Virolution, Experimental Research Center for Infectious Diseases, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto 606-8507, Japan.
Sayumi
S
Shimode
Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto 606-8507, Japan; Laboratory of Virolution, Experimental Research Center for Infectious Diseases, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto 606-8507, Japan.
Shoichi
S
Sakaguchi
Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto 606-8507, Japan; Laboratory of Virolution, Experimental Research Center for Infectious Diseases, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto 606-8507, Japan.
Rokusuke
R
Yoshikawa
Micro/Nano Technology Center, Tokai University, 411 Kitakaname, Hiratsuka, Kanagawa 259-1292, Japan.
Mahoko Ueda
MU
Takahashi
Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto 606-8507, Japan; Laboratory of Virolution, Experimental Research Center for Infectious Diseases, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto 606-8507, Japan. Electronic address: takavet@gmail.com.
Takayuki
T
Miyazawa
D000595:Amino Acid Sequence; D026962:Amino Acid Transport System ASC / Q000235:genetics; D000818:Animals; D000829:Animals, Domestic; D001483:Base Sequence; D002415:Cats; D002417:Cattle; D045744:Cell Line, Tumor; D004285:Dogs; D020077:Endogenous Retroviruses / Q000502:physiology*; D005289:Ferrets / Q000235:genetics; D015316:Genetic Therapy; D005822:Genetic Vectors; D006736:Horses / Q000235:genetics; D054643:INDEL Mutation; D051379:Mice; D008907:Mink / Q000235:genetics; D015778:Minor Histocompatibility Antigens; D008969:Molecular Sequence Data; D041681:NIH 3T3 Cells; D010802:Phylogeny; D011784:Quail / Q000235:genetics; D051381:Rats; D011991:Receptors, Virus / Q000235:genetics; D034561:Sheep, Domestic / Q000235:genetics; D034421:Sus scrofa / Q000235:genetics; D014161:Transduction, Genetic; D014759:Viral Envelope Proteins / Q000235:genetics*
D026962:Amino Acid Transport System ASC; D015778:Minor Histocompatibility Antigens; D011991:Receptors, Virus; C420506:SLC1A5 protein, human; D014759:Viral Envelope Proteins
ASCT2; Domestic mammals; Gene therapy; RD-114 virus; Receptor; Retrovirus vector