[Genetic diversity analysis of Andrographis paniculata in China based on SRAP and SNP].
In order to reveal genetic diversity of domestic Andrographis paniculata and its impact on quality, genetic backgrounds of 103 samples from 7 provinces in China were analyzed using SRAP marker and SNP marker. Genetic structures of the A. paniculata populations were estimated with Powermarker V 3.25 and Mega 6.0 software, and polymorphic SNPs were identified with CodonCode Aligner software. The results showed that the genetic distances of domestic A. paniculata germplasm ranged from 0. 01 to 0.09, and no polymorphic SNPs were discovered in coding sequence fragments of ent-copalyl diphosphate synthase. A. paniculata germplasm from various regions in China had poor genetic diversity. This phenomenon was closely related to strict self-fertilization and earlier introduction from the same origin. Therefore, genetic background had little impact on variable qualities of A. paniculata in domestic market. Mutation breeding, polyploid breeding and molecular breeding were proposed as promising strategies in germplasm innovation.
Rong
R
Chen
Xiao-Yun
XY
Wang
Yu-Ning
YN
Song
Yun-feng
YF
Zhu
Peng-liang
PL
Wang
Min
M
Li
Guo-Yue
GY
Zhong
D035685:Andrographis / Q000145:classification / Q000235:genetics*; D002681:China; D014644:Genetic Variation; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide