Comparative immunoprophylactic efficacy of Haemonchus contortus recombinant enolase (rHcENO) and Con A purified native glycoproteins in sheep.
Haemonchus contortus is the most economically important blood feeding nematode parasite of sheep and goats all over the world. Enolase in helminth parasites is a multi-functional enzyme which involves in glycolysis and host tissue invasion. In this study, the recombinant H. contortus enolase (rHcENO) was evaluated for its immunoprophylactic efficacy in sheep along with Con A purified native glycoproteins in a vaccine challenge trial. Group I and Group II experimental sheep were immunized thrice with rHcENO and Con A purified native glycoproteins along with Montanide ISA 61 VG adjuvant. The animals were challenged with 5000 L3 stage active H. contortus larvae after 21 days of third immunization. A significant increase in the IgG titre was observed in rHcENO and Con A purified native glycoproteins immunized animals as compared to the control animals. Immunoprotective efficacy of Con A purified native glycoproteins was comparatively higher than rHcENO antigen.
Department of Veterinary Parasitology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai 600007, India.
Aravindan
A
Kalyanasundaram
Department of Veterinary Parasitology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai 600007, India.
Shabnam
S
Jawahar
Department of Veterinary Parasitology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai 600007, India.
Manikkavasagan
M
Ilangopathy
Department of Veterinary Parasitology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai 600007, India.
Azahahianambi
A
Palavesam
Department of Veterinary Parasitology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai 600007, India; Translational Research Platform for Veterinary Biologicals (TRPVB), TANUVAS, II Floor, Central University Laboratory Building, Madhavaram Milk Colony, Chennai 600051, India. Electronic address: raman@tanuvas.org.in.
Muthusamy
M
Raman
D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis* / Q000097:blood; D001483:Base Sequence; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology; D003208:Concanavalin A; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology; D006023:Glycoproteins / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D006188:Haemonchiasis / Q000517:prevention & control*; D006189:Haemonchus / Q000201:enzymology* / Q000254:growth & development; D007111:Immunity, Cellular; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010270:Parasite Egg Count; D010751:Phosphopyruvate Hydratase / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D016415:Sequence Alignment; D012756:Sheep; D014612:Vaccines / Q000592:standards
D000909:Antibodies, Helminth; D006023:Glycoproteins; D011994:Recombinant Proteins; D014612:Vaccines; D003208:Concanavalin A; D010751:Phosphopyruvate Hydratase
Con A purified native glycoproteins; Enolase; Haemonchus contortus; Sheep; Vaccine; rHcENO