The Diaporthe sojae species complex: Phylogenetic re-assessment of pathogens associated with soybean, cucurbits and other field crops.
Phytopathogenic species of Diaporthe are associated with a number of soybean diseases including seed decay, pod and stem blight and stem canker and lead to considerable crop production losses worldwide. Accurate morphological identification of the species that cause these diseases has been difficult. In this study, we determined the phylogenetic relationships and species boundaries of Diaporthe longicolla, Diaporthe phaseolorum, Diaporthe sojae and closely related taxa. Species boundaries for this complex were determined based on combined phylogenetic analysis of five gene regions: partial sequences of calmodulin (CAL), beta-tubulin (TUB), histone-3 (HIS), translation elongation factor 1-α (EF1-α), and the nuclear ribosomal internal transcribed spacers (ITS). Phylogenetic analyses revealed that this large complex of taxa is comprised of soybean pathogens as well as species associated with herbaceous field crops and weeds. Diaporthe arctii, Diaporthe batatas, D. phaseolorum and D. sojae are epitypified. The seed decay pathogen D. longicolla was determined to be distinct from D. sojae. D. phaseolorum, originally associated with stem and leaf blight of Lima bean, was not found to be associated with soybean. A new species, Diaporthe ueckerae on Cucumis melo, is introduced with description and illustrations.
Systematic Mycology and Microbiology Laboratory, United States Department of Agriculture - Agricultural Research Service, Beltsville, MD 20705, USA; Institute of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, 57100, Thailand; School of Science, Mae Fah Luang University, Chiang Rai, 57100, Thailand.
Dhanushka
D
Udayanga
Systematic Mycology and Microbiology Laboratory, United States Department of Agriculture - Agricultural Research Service, Beltsville, MD 20705, USA. Electronic address: Lisa.Castlebury@ars.usda.gov.
Lisa A
LA
Castlebury
Systematic Mycology and Microbiology Laboratory, United States Department of Agriculture - Agricultural Research Service, Beltsville, MD 20705, USA.
Amy Y
AY
Rossman
Institute of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, 57100, Thailand; School of Science, Mae Fah Luang University, Chiang Rai, 57100, Thailand.
Ekachai
E
Chukeatirote
Institute of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, 57100, Thailand; School of Science, Mae Fah Luang University, Chiang Rai, 57100, Thailand; Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, People's Republic of China; World Agroforestry Centre, East and Central Asia, Kunming 650201, People's Republic of China.
Kevin D
KD
Hyde
D001203:Ascomycota / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D005656:Fungal Proteins / Q000235:genetics; D019684:Magnoliopsida / Q000382:microbiology*; D008969:Molecular Sequence Data; D020648:Peptide Elongation Factor 1 / Q000235:genetics; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D013025:Glycine max / Q000382:microbiology*; D014404:Tubulin / Q000235:genetics; D014774:Virulence
D005656:Fungal Proteins; D020648:Peptide Elongation Factor 1; D014404:Tubulin
Genealogical concordance; Glycine max; Phomopsis seed decay; Pod and stem blight; Soft rot of cantaloupe