Detection of Interferon gamma using graphene and aptamer based FET-like electrochemical biosensor.
One of the primary goals in the scientific community is the specific detection of proteins for the medical diagnostics and biomedical applications. Interferon-gamma (IFN-γ) is associated with the tuberculosis susceptibility, which is one of the major health problems globally. We have therefore developed a DNA aptamer-based electrochemical biosensor that is used for the detection of IFN-γ with high selectivity and sensitivity. A graphene monolayer-based FET-like structure is incorporated on a PDMS substrate with the IFN-γ aptamer attached to graphene. Addition of target molecule induces a change in the charge distribution in the electrolyte, resulting in increase in electron transfer efficiency that was actively sensed by monitoring the change in current from the device. Change in current appears to be highly sensitive to the IFN-γ concentrations ranging from nanomolar (nM) to micromolar (μM) range. The detection limit of our IFN-γ electrochemical biosensor is found to be 83 pM. Immobilization of aptamer on graphene surface is verified using unique structural approach by Atomic Force Microscopy. Such simple and sensitive electrochemical biosensor has potential applications in infectious disease monitoring, immunology and cancer research in the future.
Department of Electrical and Computer Engineering, University of Illinois, Chicago, IL 60607, USA. Electronic address: sfarid3@uic.edu.
Sidra
S
Farid
Department of Bioengineering, University of Illinois, Chicago, IL 60607, USA.
Xenia
X
Meshik
Department of Electrical and Computer Engineering, University of Illinois, Chicago, IL 60607, USA.
Min
M
Choi
Department of Electrical and Computer Engineering, University of Illinois, Chicago, IL 60607, USA.
Souvik
S
Mukherjee
Department of Electrical and Computer Engineering, University of Illinois, Chicago, IL 60607, USA.
Yi
Y
Lan
Department of Bioengineering, University of Illinois, Chicago, IL 60607, USA.
Devanshi
D
Parikh
Department of Electrical and Computer Engineering, University of Illinois, Chicago, IL 60607, USA.
Shripriya
S
Poduri
Department of Bioengineering, University of Illinois, Chicago, IL 60607, USA.
Undarmaa
U
Baterdene
Department of Electrical and Computer Engineering, University of Illinois, Chicago, IL 60607, USA.
Ching-En
CE
Huang
Department of Chemical Engineering and Materials Science, University of California, Irvine, CA 92697, USA.
Yung Yu
YY
Wang
Department of Electrical Engineering and Computer Science, University of California, Irvine, CA 92697, USA.
Peter
P
Burke
Department of Electrical and Computer Engineering, University of Illinois, Chicago, IL 60607, USA; Department of Physics, University of Illinois, Chicago, IL 60607, USA.
Mitra
M
Dutta
Department of Electrical and Computer Engineering, University of Illinois, Chicago, IL 60607, USA; Department of Bioengineering, University of Illinois, Chicago, IL 60607, USA; Department of Physics, University of Illinois, Chicago, IL 60607, USA.
Michael A
MA
Stroscio
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007371:Interferon-gamma / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014173:Transistors, Electronic
D052157:Aptamers, Nucleotide; D007371:Interferon-gamma
Aptamer; Atomic Force Microscopy; Graphene; Interferon-gamma