Effect of toothpaste with nano-sized trimetaphosphate on dental caries: In situ study.
OBJECTIVE
This in situ study was to evaluate the remineralizing effect of a fluoride toothpaste supplemented with nano-sized sodium trimetaphosphate (TMP).


METHODS
This blind and cross-over study was performed in 4 phases of 3 days each. Twelve subjects used palatal appliances containing four bovine enamel blocks with artificial caries lesions. Volunteers were randomly assigned into the following treatment groups: Placebo (without F and TMP); 1100 ppm F (1100), 1100 supplemented with 3% micrometric TMP (1100 TMP) and with nano-sized TMP (1100 TMPnano). Volunteers were instructed to brush their natural teeth with the palatal appliances in the mouth during 1min (3 times/day), so that blocks were treated with natural slurries of toothpastes. After each phase, the percentage of surface hardness recovery (%SHR), integrated mineral recovery (IMR) and integrated differential mineral area profile (ΔIMR) in enamel lesions were calculated. F in enamel was also determined. Data were analyzed by ANOVA and Student-Newman-Keuls test.


RESULTS
Enamel surface became 20% harder when treated with 1100 TMPnano in comparison with 1100 (p<0.001). 1100 TMPnano showed remineralizing capacity (IMR; ΔIMR) 66% higher when compared with 1100 TMP (p<0.001). Enamel F uptake in the 1100 TMPnano group was 2-fold higher when compared to its counterpart without TMP (p<0.001).


CONCLUSIONS
The addition of 3% TMPnano to a conventional toothpaste was able to promote an additional remineralizing effect of artificial caries lesions.


CONCLUSIONS
Toothpaste containing 1100 ppm F associated with TMPnano showed a potential of higher remineralization to 1100 ppm F and 1100 ppm F micrometric TMP.
Araçatuba Dental School, Univ. Estadual Paulista (UNESP), Department of Pediatric Dentistry and Public Health, Rua José Bonifácio 1193, Araçatuba, SP CEP 16015-050, Brazil.
Marcelle
M
Danelon
Araçatuba Dental School, Univ. Estadual Paulista (UNESP), Department of Pediatric Dentistry and Public Health, Rua José Bonifácio 1193, Araçatuba, SP CEP 16015-050, Brazil.
Juliano Pelim
JP
Pessan
LIEC-Department of Chemistry, Federal University of São Carlos (UFSCar), 13565-905 São Carlos/São Paulo, Brazil.
Francisco Nunes Souza
FN
Neto
LIEC-Department of Chemistry, Federal University of São Carlos (UFSCar), 13565-905 São Carlos/São Paulo, Brazil.
Emerson Rodrigues
ER
de Camargo
Araçatuba Dental School, Univ. Estadual Paulista (UNESP), Department of Pediatric Dentistry and Public Health, Rua José Bonifácio 1193, Araçatuba, SP CEP 16015-050, Brazil. Electronic address: adelbem@foa.unesp.br.
Alberto Carlos Botazzo
AC
Delbem
D000328:Adult; D000818:Animals; D002417:Cattle; D018592:Cross-Over Studies; D003731:Dental Caries / Q000188:drug therapy*; D005260:Female; D005460:Fluorides, Topical / Q000008:administration & dosage; D006244:Hardness / Q000187:drug effects; D006801:Humans; D008297:Male; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D011122:Polyphosphates / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D014089:Tooth Remineralization; D014100:Toothpastes / Q000737:chemistry / Q000627:therapeutic use*; D055815:Young Adult
D005460:Fluorides, Topical; D011122:Polyphosphates; D014100:Toothpastes; C009293:trimetaphosphoric acid
Nano-sized; Phosphates; Tooth remineralization; Toothpastes