An optimized enrichment technique for the isolation of Arthrobacter bacteriophage species from soil sample isolates.
Bacteriophage isolation from environmental samples has been performed for decades using principles set forth by pioneers in microbiology. The isolation of phages infecting Arthrobacter hosts has been limited, perhaps due to the low success rate of many previous isolation techniques, resulting in an underrepresented group of Arthrobacter phages available for study. The enrichment technique described here, unlike many others, uses a filtered extract free of contaminating bacteria as the base for indicator bacteria growth, Arthrobacter sp. KY3901, specifically. By first removing soil bacteria the target phages are not hindered by competition with native soil bacteria present in initial soil samples. This enrichment method has resulted in dozens of unique phages from several different soil types and even produced different types of phages from the same enriched soil sample isolate. The use of this procedure can be expanded to most nutrient rich aerobic media for the isolation of phages in a vast diversity of interesting host bacteria.
Biology Department, University of the Sciences.
Trevor
T
Cross
Biology Department, Arcadia University.
Courtney
C
Schoff
Biology Department, Immaculata University.
Dylan
D
Chudoff
Biology Department, Immaculata University.
Libby
L
Graves
Biology/Clinical Laboratory Science, Neumann University.
Haley
H
Broomell
Biology/Clinical Laboratory Science, Neumann University.
Katrina
K
Terry
Science Department, Cabrini College.
Jennifer
J
Farina
Science Department, Cabrini College.
Alexandra
A
Correa
Science Department, Cabrini College.
David
D
Shade
Science Department, Cabrini College; dunbardavid75@gmail.com.
David
D
Dunbar
D001173:Arthrobacter / Q000821:virology*; D001435:Bacteriophages / Q000302:isolation & purification*; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D014773:Virology / Q000379:methods
D012987:Soil