Relationship Between Ebola Virus Real-Time Quantitative Polymerase Chain Reaction-Based Threshold Cycle Value and Virus Isolation From Human Plasma.
We performed a longitudinal analysis of plasma samples obtained from 4 patients with Ebola virus (EBOV) disease (EVD) to determine the relationship between the real-time quantitative reverse transcriptase polymerase chain reaction (qRT-PCR)-based threshold cycle (Ct) value and the presence of infectious EBOV. EBOV was not isolated from plasma samples with a Ct value of >35.5 or >12 days after onset of symptoms. EBOV was not isolated from plasma samples in which anti-EBOV nucleoprotein immunoglobulin G was detected. These data demonstrate the utility of interpreting qRT-PCR results in the context of the course of EBOV infection and associated serological responses for patient-management decisions.
Viral Special Pathogens Branch, Division of High-Consequence Pathogens and Pathology, Centers for Disease Control and Prevention.
Jessica R
JR
Spengler
Viral Special Pathogens Branch, Division of High-Consequence Pathogens and Pathology, Centers for Disease Control and Prevention Division of Pediatric Infectious Diseases, Emory University, Atlanta, Georgia.
Anita K
AK
McElroy
Viral Special Pathogens Branch, Division of High-Consequence Pathogens and Pathology, Centers for Disease Control and Prevention Division of Pediatric Infectious Diseases, Emory University, Atlanta, Georgia.
Jessica R
JR
Harmon
Viral Special Pathogens Branch, Division of High-Consequence Pathogens and Pathology, Centers for Disease Control and Prevention.
Ute
U
Ströher
Viral Special Pathogens Branch, Division of High-Consequence Pathogens and Pathology, Centers for Disease Control and Prevention.
Stuart T
ST
Nichol
Viral Special Pathogens Branch, Division of High-Consequence Pathogens and Pathology, Centers for Disease Control and Prevention.
Christina F
CF
Spiropoulou
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D029043:Ebolavirus / Q000235:genetics* / Q000276:immunology; D019142:Hemorrhagic Fever, Ebola / Q000276:immunology / Q000821:virology*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D008137:Longitudinal Studies; D009698:Nucleoproteins / Q000276:immunology; D010949:Plasma / Q000276:immunology / Q000821:virology*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D014709:Vero Cells
D007074:Immunoglobulin G; D009698:Nucleoproteins
Ebola; qRT-PCR; virus isolation