Entry of Ebola Virus is an Asynchronous Process.
Ebola virus (EBOV) is responsible for a severe fever with a high mortality rate. The diverse nature of the attachment of the virus to the cell surface, the initial step of virus entry, raises questions concerning the kinetics of the virus internalization process. We investigated EBOV entry kinetics using the activity of a particular monoclonal antibody that neutralizes virus infectivity. We demonstrate that inoculation of cells with EBOV results in an asynchronous entry process, as revealed by the ability of the virus to remain in a cell-bound state for an extended period of time before it is internalized.
Laboratory Molecular Basis of Viral Pathogenicity, International Centre for Research in Infectiology (CIRI), INSERM U1111, Université de Lyon, Université Claude Bernard Lyon 1, Ecole Normale Supérieure de Lyon, France.
Olivier
O
Reynard
Laboratory Molecular Basis of Viral Pathogenicity, International Centre for Research in Infectiology (CIRI), INSERM U1111, Université de Lyon, Université Claude Bernard Lyon 1, Ecole Normale Supérieure de Lyon, France.
Viktor E
VE
Volchkov
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D057134:Antibodies, Neutralizing / Q000276:immunology; D002460:Cell Line; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D029043:Ebolavirus / Q000276:immunology / Q000472:pathogenicity* / Q000502:physiology*; D019142:Hemorrhagic Fever, Ebola / Q000276:immunology / Q000821:virology*; D006801:Humans; D007700:Kinetics; D014709:Vero Cells; D053586:Virus Internalization
D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing
Ebola virus; family Filoviridae; glycoprotein GP; virus attachment; virus entry kinetics; virus neutralizing antibody