Feed supplemented with byproducts from olive oil mill wastewater processing increases antioxidant capacity in broiler chickens.
In the present study, a ceramic membrane microfiltration method was used for the separation of two liquid products, the downstream permeate and the upstream retentate, from olive mill wastewater (OMWW). These liquid products were examined for their antioxidant activity by incorporating them into broilers' feed. Twenty four broilers 13 d old were divided into two feeding groups receiving supplementation with OMWW retentate or permeate for 37 d. Blood was drawn at 17, 27 and 37 d, while tissues (muscle, heart, liver) were collected at 37 d. The antioxidant effects were assessed by measuring oxidative stress biomarkers in blood and tissues. The results showed that broilers given feed supplemented with OMWW retentate or permeate had significantly lower protein oxidation and lipid peroxidation levels and higher total antioxidant capacity in plasma and tissues compared to control group. In both OMWW groups, catalase activity in erythrocytes and tissues was significantly increased compared to control group. OMWW retentate administration increased significantly GSH in erythrocytes in broilers with low GSH, although both OMWW products significantly reduced GSH in broilers with high GSH. Thus, it has been demonstrated for the first time that supplementation with OMWW processing residues could be used for enhancing broilers' redox status.
Department of Biochemistry and Biotechnology, University of Thessaly, Ploutonos 26 and Aiolou st., 41221 Larissa, Greece; Department of Biosystem Engineering, Technical Education Institute of Thessaly, 41110 Larissa, Greece.
Konstantinos
K
Gerasopoulos
Department of Biochemistry and Biotechnology, University of Thessaly, Ploutonos 26 and Aiolou st., 41221 Larissa, Greece.
Dimitrios
D
Stagos
Department of Biosystem Engineering, Technical Education Institute of Thessaly, 41110 Larissa, Greece.
Stylianos
S
Kokkas
Department of Biosystem Engineering, Technical Education Institute of Thessaly, 41110 Larissa, Greece. Electronic address: petrotos@teilar.gr.
Konstantinos
K
Petrotos
Department of Biosystem Engineering, Technical Education Institute of Thessaly, 41110 Larissa, Greece.
Dimitrios
D
Kantas
Department of Biosystem Engineering, Technical Education Institute of Thessaly, 41110 Larissa, Greece.
Panagiotis
P
Goulas
Department of Biochemistry and Biotechnology, University of Thessaly, Ploutonos 26 and Aiolou st., 41221 Larissa, Greece. Electronic address: dkouret@uth.gr.
Dimitrios
D
Kouretas
D000821:Animal Feed; D000818:Animals; D000975:Antioxidants / Q000378:metabolism* / Q000494:pharmacology; D015415:Biomarkers / Q000097:blood; D002374:Catalase / Q000097:blood; D002645:Chickens / Q000378:metabolism*; D005374:Filtration / Q000295:instrumentation / Q000379:methods; D005978:Glutathione / Q000097:blood; D015227:Lipid Peroxidation / Q000187:drug effects; D000069463:Olive Oil; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D014866:Waste Products; D062065:Wastewater
D000975:Antioxidants; D015415:Biomarkers; D002374:Catalase; D005978:Glutathione; D000069463:Olive Oil; D014866:Waste Products; D062065:Wastewater
Antioxidant feed; Ceramic membrane microfiltration; Chickens; Natural products; Olive mill wastewater; Oxidative stress