In Vitro Behavior of Primary Human Osteoblasts Onto Microrough Titanium Surface.
OBJECTIVE
The aim of this study was to evaluate in vitro the behavior and the biocompatibility of primary human osteoblasts (HOs) grown onto different implant surface.


METHODS
HOs were cultured onto sandblasted/acid-etched (control group) and sandblasted/acid-etched followed by coating with inorganic ions (test group) experimental titanium discs. At established times, SEM analysis, LDH assay, MTT assay, and enzyme-linked immunosorbent assay for type 1 collagen, interleukin (IL)-6, and PGE2 secretion were performed.


RESULTS
Both surfaces promote HOs adhesion and proliferation. After 21 days, cells on test surfaces are well spread, flattened, and attached by cellular extensions, whereas cells on control discs appear mainly elongated. Lower LDH levels and higher values of MTT assay are recorded for cells on test respect to control surfaces at each experimental time. Type 1 collagen release increases until 14 days, significantly decreasing at day 21 in cells grown on both surfaces. IL-6 and PGE2 secretion shows a peak in control group samples at day 7, whereas their levels do not significantly modify in both groups at days 14 and 21.


CONCLUSIONS
Results indicate that the test group surface is more biocompatible, well tolerated, and suitable for supporting osteoblasts growth and proliferation.
*Research Fellow, Department of Pharmacy, University of "G. d'Annunzio," Chieti, Italy. †PhD Student, Department of Pharmacy, University of "G. d'Annunzio," Chieti, Italy. ‡Research Fellow, Department of Pharmacy, University of "G. d'Annunzio," Chieti, Italy. §Researcher, Department of Pharmacy, University of "G. d'Annunzio," Chieti, Italy. ||Researcher, Department of Biomedical Sciences, University of Padova, Padova, Italy. ¶Professor, Department of Medicine and Aging Sciences, University of "G. d'Annunzio," Chieti, Italy. #Professor, Department of Pharmacy, University of "G. d'Annunzio," Chieti, Italy. **Professor, Department of Medical, Oral and Biotechnological Sciences, University "G. d'Annunzio," Chieti, Italy.
Vincenzo Luca
VL
Zizzari
Guya Diletta
GD
Marconi
Marianna
M
De Colli
Susi
S
Zara
Barbara
B
Zavan
Vincenzo
V
Salini
Antonella
A
Fontana
Amelia
A
Cataldi
Adriano
A
Piattelli
D000469:Alkaline Phosphatase / Q000096:biosynthesis; D001672:Biocompatible Materials; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis; D003758:Dental Implantation, Endosseous / Q000379:methods*; D015921:Dental Implants; D015232:Dinoprostone / Q000378:metabolism; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis; D016348:Osseointegration / Q000502:physiology*; D010006:Osteoblasts / Q000166:cytology*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry
D001672:Biocompatible Materials; D024042:Collagen Type I; D015921:Dental Implants; C508600:IL6 protein, human; D015850:Interleukin-6; D014025:Titanium; D000469:Alkaline Phosphatase; D015232:Dinoprostone