Serum reactivity against herpes simplex virus type 1 UL48 protein in Behçet's disease patients and a Behçet's disease-like mouse model.
Herpes simplex virus (HSV) infection is a possible pathogenic factor in Behçet's disease (BD). Using proteomics analysis, this study detected a target HSV protein. Serum IgA and IgG reactivities against the identified protein were evaluated in patients with BD and in BD-like mice. A total of 4 protein bands generated by immunoprecipitation were analysed by proteomics, and HSV UL48 was commonly found in both IgA- and IgG-reactive protein bands. Compared with controls, patients with BD and BD-like mice exhibited higher titres of IgA reacting with recombinant HSV UL48 protein. Further proteomics analysis revealed that human heat shock cognate 71 kDa protein (Hsc71) is a cross-reacting target antigen against anti-HSV UL48 antibody. In addition, our data demonstrated a very strong association between serum IgG reactivity against recombinant human Hsc71 and recombinant HSV UL48 in patients with BD. We suggest that HSV infection and impaired human Hsc71 activity may be associated with the activation of autoreactive lymphocytes.
Department of Dermatology and Cutaneous Biology Research Institute, Yonsei University College of Medicine, Seoul, Korea.
Zhenlong
Z
Zheng
Seonghyang
S
Sohn
Keun Jae
KJ
Ahn
Dongsik
D
Bang
Sung Bin
SB
Cho
D000328:Adult; D000818:Animals; D001528:Behcet Syndrome / Q000097:blood*; D016022:Case-Control Studies; D003429:Cross Reactions; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000276:immunology; D005260:Female; D050883:HSC70 Heat-Shock Proteins / Q000276:immunology*; D018259:Herpesvirus 1, Human / Q000276:immunology*; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood*; D007074:Immunoglobulin G / Q000097:blood*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D055806:Microvessels / Q000276:immunology; D008875:Middle Aged; D011994:Recombinant Proteins / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology*; D055815:Young Adult
D050883:HSC70 Heat-Shock Proteins; C494371:HSPA8 protein, human; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D011994:Recombinant Proteins; C000591971:UL48 protein, human cytomegalovirus; D014764:Viral Proteins