Novel insights into structure-function mechanism and tissue-specific expression profiling of full-length dxr gene from Cymbopogon winterianus.
The 1-deoxy-d-xylulose-5-phosphate reductoisomerase (DXR; EC1.1.1.267), an NADPH-dependent reductase, plays a pivotal role in the methylerythritol 4-phosphate pathway (MEP), in the conversion of 1-deoxy-d-xylulose-5-phosphate (DXP) into MEP. The sheath and leaf of citronella (Cymbopogon winterianus) accumulates large amount of terpenes and sesquiterpenes with proven medicinal value and economic uses. Thus, sequencing of full length dxr gene and its characterization seems to be a valuable resource in metabolic engineering to alter the flux of isoprenoid active ingredients in plants. In this study, full length DXR from citronella was characterized through in silico and tissue-specific expression studies to explain its structure-function mechanism, mode of cofactor recognition and differential expression. The modelled DXR has a three-domain architecture and its active site comprised of a cofactor (NADPH) binding pocket and the substrate-binding pocket. Molecular dynamics simulation studies indicated that DXR model retained most of its secondary structure during 10 ns simulation in aqueous solution. The modelled DXR superimposes well with its closest structural homolog but subtle variations in the charge distribution over the cofactor recognition site were noticed. Molecular docking study revealed critical residues aiding tight anchoring NADPH within the active pocket of DXR. Tissue-specific differential expression analysis using semi-quantitative RT-PCR and qRT-PCR in various tissues of citronella plant revealed distinct differential expression of DXR. To our knowledge, this is the first ever report on DXR from the important medicinal plant citronella and further characterization of this gene will open up better avenues for metabolic engineering of secondary metabolite pathway genes from medicinal plants in the near future.
Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat 785013, Assam, India.
Kamalakshi
K
Devi
Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat 785013, Assam, India ; Regional Medical Research Centre (ICMR), Chandrasekharpur, Nandankanan Road, Bhubaneswar 751023, Odisha, India.
Budheswar
B
Dehury
Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat 785013, Assam, India ; School of Biological Sciences, College of Life Science & Medicine, University of Aberdeen, Aberdeen AB24 3UU, Scotland, UK.
Munmi
M
Phukon
Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat 785013, Assam, India.
Mahendra Kumar
MK
Modi
Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat 785013, Assam, India.
Priyabrata
P
Sen
Citronella; DXR; Docking; Molecular dynamics simulation; Molecular modelling