Somatic cell count and milk neutrophil viability of dairy heifers with specific CXCR1 genotypes following experimental intramammary infection with Staphylococcus chromogenes originating from milk.
Previous observational studies suggest an association between polymorphism c.980A>G in the CXCR1 gene, encoding the chemokine (C-X-C motif) receptor 1, and the innate immunity and infection status of the mammary gland. Mammary glands of eight Holstein heifers were experimentally infected with a Staphylococcus chromogenes isolate originating from a chronic intramammary infection (IMI) to study differences between CXCR1 genotypes c.980AG and c.980GG. Quarters from heifers with genotypes c.980AG and c.980GG developed subclinical mastitis but showed differences in the early response at 6-18 h post challenge. Bacterial count at 18 h post challenge tended to be higher in quarters from c.980AG heifers compared to c.980GG heifers. Somatic cell count (SCC) was higher at 6 h post challenge and tended to be higher at 9 h post challenge in c.980AG heifers compared to c.980GG heifers. Milk production decreased similarly. Milk neutrophils of c.980AG heifers showed more apoptosis at 9 h post challenge and tended to show more necrosis at 6, 9 and 12 h post challenge than c.980GG heifers. Differences were less pronounced in the later stage (>18 h) of infection. The results demonstrate that CXCR1 polymorphism can influence SCC and milk neutrophil viability following experimental IMI.
M-team and Mastitis and Milk Quality Research Unit, Department of Reproduction, Obstetrics, and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, Merelbeke 9820, Belgium. Electronic address: Joren.Verbeke@UGent.be.
Joren
J
Verbeke
M-team and Mastitis and Milk Quality Research Unit, Department of Reproduction, Obstetrics, and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, Merelbeke 9820, Belgium.
Kristine
K
Piccart
M-team and Mastitis and Milk Quality Research Unit, Department of Reproduction, Obstetrics, and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, Merelbeke 9820, Belgium.
Sofie
S
Piepers
Animal Genetics Laboratory, Department of Nutrition, Genetics, and Ethology, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, Merelbeke 9820, Belgium.
Mario
M
Van Poucke
Animal Genetics Laboratory, Department of Nutrition, Genetics, and Ethology, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, Merelbeke 9820, Belgium.
Luc
L
Peelman
M-team and Mastitis and Milk Quality Research Unit, Department of Reproduction, Obstetrics, and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, Merelbeke 9820, Belgium.
Anneleen
A
De Visscher
M-team and Mastitis and Milk Quality Research Unit, Department of Reproduction, Obstetrics, and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, Merelbeke 9820, Belgium.
Sarne
S
De Vliegher
D000818:Animals; D002417:Cattle; D002470:Cell Survival; D005260:Female; D008321:Mammary Glands, Animal / Q000276:immunology; D008414:Mastitis, Bovine / Q000378:metabolism*; D008892:Milk / Q000166:cytology*; D009504:Neutrophils / Q000502:physiology*; D023062:Receptors, Interleukin-8A / Q000235:genetics / Q000378:metabolism*; D013203:Staphylococcal Infections / Q000662:veterinary*; D013210:Staphylococcus / Q000145:classification*
D023062:Receptors, Interleukin-8A
CXCR1 polymorphism; Experimental intramammary infection; Staphylococcus chromogenes