Administration of TLR7 agonist, resiquimod, in different types of chicken induces a mixed Th1 and Th2 response in the peripheral blood mononuclear cells.
This study evaluated the variation in immune response in peripheral blood mononuclear cells (PBMCs) of broiler, White Leghorn (WL) and Kadaknath breeds of chicken following administration of TLR7 agonist, resiquimod (R-848). Expression of different immune related genes viz., interferon-β (IFN-β), IFN-γ, IL-1β, IL-4, TLR7 and iNOS was assessed by quantitative real time PCR over a period of 24 h. The results indicated that there was a significant up-regulation in the relative expression of immune response genes post R-848 administration (P < 0.01). In conclusion, the transcriptional expression of IFN-β, IFN-γ, IL-1β, IL-4, iNOS and TLR7 genes in the PBMCs was significantly up-regulated over 24 h in broiler, WL and Kadaknath breeds of birds after the administration of R-848. Overall, R-848 induced a mixed Th1 and Th2 response in PBMCs of chicken origin ex vivo.
Immunology Section, Indian Veterinary Research Institute (IVRI), Izatnagar, Bareilly, Uttar Pradesh 243 122, India.
Arunsaravanakumar
A
Annamalai
Immunology Section, Indian Veterinary Research Institute (IVRI), Izatnagar, Bareilly, Uttar Pradesh 243 122, India. Electronic address: dearsaromib@yahoo.com.
Saravanan
S
Ramakrishnan
Immunology Section, Indian Veterinary Research Institute (IVRI), Izatnagar, Bareilly, Uttar Pradesh 243 122, India.
Swati
S
Sachan
Immunology Section, Indian Veterinary Research Institute (IVRI), Izatnagar, Bareilly, Uttar Pradesh 243 122, India.
Bal Krishan
BK
Sharma
Immunology Section, Indian Veterinary Research Institute (IVRI), Izatnagar, Bareilly, Uttar Pradesh 243 122, India.
B S
BS
Anand Kumar
Immunology Section, Indian Veterinary Research Institute (IVRI), Izatnagar, Bareilly, Uttar Pradesh 243 122, India.
Vimal
V
Kumar
Immunology Section, Indian Veterinary Research Institute (IVRI), Izatnagar, Bareilly, Uttar Pradesh 243 122, India.
Surendra Kumar
SK
Badasara
Division of Animal Biochemistry, IVRI, Izatnagar, Bareilly, Uttar Pradesh 243 122, India.
Ajay
A
Kumar
Division of Parasitology, IVRI, Izatnagar, Bareilly, Uttar Pradesh 243 122, India.
B C
BC
Saravanan
Division of Animal Reproduction, IVRI, Izatnagar, Bareilly, Uttar Pradesh 243 122, India.
Narayanan
N
Krishnaswamy
D000818:Animals; D002645:Chickens / Q000235:genetics / Q000276:immunology*; D007093:Imidazoles / Q000494:pharmacology*; D007113:Immunity, Innate; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology*; D018417:Th1 Cells / Q000187:drug effects / Q000276:immunology*; D018418:Th2 Cells / Q000187:drug effects / Q000276:immunology*; D051199:Toll-Like Receptor 7 / Q000819:agonists*
D007093:Imidazoles; D051199:Toll-Like Receptor 7; C402365:resiquimod
Chicken; Immune response genes; PBMCs; Resiquimod; Toll-like receptors