[Effect of Inhibiting NGAL Gene Expression on A549 Lung Cancer Cell Migration and Invasion].
BACKGROUND
To detect the expression of neutrophil gelatinase-assoeiated lipocalin (NGAL) in the different differentiations of lung cancer tissues and to study the mechanism of invasion of A549 cells affected by NGAL.


METHODS
The expression of NGAL was detected by immunochemistry in lung cancer tissue and the tissue around edge of the cancer. The effect of NGAL expression on A549 cells was observed by using qRT-PCR and Western blot. The abilities of invasion and metastasis were evaluated by transwell invasion and migration assay, and cell scratch assay in vitro. The protein expression of E-cadherin, Vimentin was measured by immunofluoresence and Western blot.


RESULTS
The positive expression rate of NGAL was 76.32% (58/76) in the lung cancer, 13.3% (4/30) in adjacent tissue by immunochemistry. NGAL expression levels in the lung cancer tissues were significantly higher than that in adjacent tissues. The rate of migration and invasion in NGAL-siRNA group was 60.4% ± 6.4% compared to 50.5% ± 4.4% in the control group, there was a significant difference (P < 0.05). Vimentin was suppressed, and E-cadherin was upregulated when NGAL was inhibited. MMP-2 and MMP-9 decreased when NGAL was knocked down.


CONCLUSIONS
The expression level of NGAL is highly expressed in lung cancer. NGAL may be one of important indicators involved in lung cancer infiltrated and transferred. NGAL might be one of potential targets for lung cancer treatment.
Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China;Department of Cardiothoracic Surgery, the First Affiliated Hospital of General Hospital of Chinese People's Liberation Army, Beijing 100048, China.
Jian
J
Tang
Department of Chest Surgery, General Hospital of Chinese People's Liberation Army, Beijing 100853, China.
Jie
J
Li
Department of Cardiothoracic Surgery, the First Affiliated Hospital of General Hospital of Chinese People's Liberation Army, Beijing 100048, China.
Shaojun
S
Li
Department of Cardiothoracic Surgery, the First Affiliated Hospital of General Hospital of Chinese People's Liberation Army, Beijing 100048, China.
Jingbo
J
Li
Department of Cardiothoracic Surgery, the First Affiliated Hospital of General Hospital of Chinese People's Liberation Army, Beijing 100048, China.
Changhai
C
Yu
Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China.
Chengze
C
Wei
D000209:Acute-Phase Proteins / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D000071068:Lipocalin-2; D054834:Lipocalins / Q000235:genetics* / Q000378:metabolism; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D009361:Neoplasm Invasiveness; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D014746:Vimentin / Q000235:genetics / Q000378:metabolism
D000209:Acute-Phase Proteins; C489506:LCN2 protein, human; D000071068:Lipocalin-2; D054834:Lipocalins; D011518:Proto-Oncogene Proteins; D034741:RNA, Small Interfering; D014746:Vimentin