ADSCs on PLLA/PCL Hybrid Nanoscaffold and Gelatin Modification: Cytocompatibility and Mechanical Properties.
BACKGROUND
Development of tissue engineering and regenerative medicine has led to designing scaffolds and their modification to provide a better microenvironment which mimics the natural niche of the cells. Gelatin surface modification was applied to improve scaffold flexibility and cytocompatibility.


METHODS
PLLA/PCL aligned fibrous scaffold was fabricated using electrospinning method. ADSCs were seeded after O2 plasma treatment and gelatin coating of the scaffolds. The morphological and mechanical properties of blends were assessed by Scanning Electron Microscopy (SEM), tensile test and ATR-FTIR. The cells proliferation was evaluated by MTT assay.


RESULTS
Based on the results, it is supposed that gelatin coating is a brilliant method of surface modification which significantly increases the mechanical properties of scaffold without any changes on the construction or on the direction of nanofibers which conducts cell's elongation. MTT analysis exhibited that ADSCs attachment, viability and proliferation significantly (p < 0.05) increased after gelatin treatment.


CONCLUSIONS
Gelatin surface modification is a highly beneficial method to improve cytocompatibility, flexibility and mechanical features of the scaffolds which doesn't affect the nanofibers construction. Proliferation of Adipose Derived Stem Cells (ADSCs) as a remarkable source of stem cells was investigated for the first time on PLLA/PCL hybrid scaffold.
Department of Biology, Faculty of Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Maedeh
M
Mashhadikhan
Department of Hematology and Blood Banking, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Masoud
M
Soleimani
Department of Biology, Faculty of Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Kazem
K
Parivar
Department of Biology, Faculty of Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Parichehr
P
Yaghmaei
Gelatin; Tissue engineering; Tissue scaffold