Interethnic relationships of CYP2D6 variants in native and Mestizo populations sharing the same ecosystem.
OBJECTIVE
To analyze the distribution of CYP2D6 variants in two ethnically-related Mexican Native and Mestizo populations cohabitating the same econiche and their relationships with a distant Mestizo community.


METHODS
314 volunteers were genotyped for CYP2D6 gene variants (*2, *3, *4, *6, *10, *13, *17, *35 and *41) using predesigned TaqMan probes. CYP2D6*5 and CYP2D6 wtxN were assessed by XL-PCR.


RESULTS
CYP2D6*1, *2, *4 and *10 variants represented above 80.9% of total alleles. Chiapanecan communities showed low allele diversity compared with the northeastern population. Principal component analyses demonstrated clustering of both Mestizo populations. Variants associated to ultrarapid and poor metabolism were rare in Natives.


CONCLUSIONS
Sharing of CYP2D6 alleles in both Chiapanecan populations suggests an ongoing gene-flow. Original submitted 8 December 2014; Revision submitted 13 February 2015.
Yadira Xitlalli
YX
Perez-Paramo
2Centro de Investigación en Matemáticas, Monterrey, Mexico.
Francisco
F
Hernandez-Cabrera
4University of Extremadura, Plasencia, Spain.
Pedro
P
Dorado
Adrian
A
Llerena
5Clínica Esquipulas, San Cristóbal de las Casas, Mexico.
Sergio
S
Muñoz-Jimenez
Rocio
R
Ortiz-Lopez
Augusto
A
Rojas-Martinez
D019389:Cytochrome P-450 CYP2D6 / Q000235:genetics*; D017753:Ecosystem; D005006:Ethnicity / Q000208:ethnology* / Q000235:genetics*; D005787:Gene Frequency / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics*; D006801:Humans; D007198:Indians, North American / Q000208:ethnology* / Q000235:genetics*; D008800:Mexico / Q000208:ethnology; D011159:Population Surveillance / Q000379:methods
D019389:Cytochrome P-450 CYP2D6
CYP2D6; Mestizo; Native American; PCA analysis; population pharmacogenetics