[Recellularization of tissue engineered vascular grafts in perfusion bioreactor].
Small diameter tissue engineered vascular grafts could be a potential solution to the shortage of vascular substitutes in reconstructive cardiovascular surgery. Previously, we have developed a decellularization method for human umbilical arteries, which could be used as a scaffold in vascular tissue engineering. Objective of the study was to optimize the recellelularization of decellularized scaffolds with mesenchymal stem cells. In the study, the possibility of cell growth on decellularized vessel has been shown. We also has proved that the use of perfusion-bioreactor improves the results of recellularization.
A S
AS
Nasredinov
S V
SV
Anisimov
V N
VN
Vavilov
M V
MV
Puzanov
D I
DI
Kurapeev
D000818:Animals; D019149:Bioreactors; D001807:Blood Vessel Prosthesis; D002448:Cell Adhesion; D049109:Cell Proliferation; D006801:Humans; D007260:Infusion Pumps; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D010477:Perfusion; D061251:Primary Cell Culture; D051381:Rats; D017208:Rats, Wistar; D046509:Tissue Culture Techniques; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014469:Umbilical Arteries / Q000737:chemistry* / Q000648:ultrastructure