High Molecular Weight Typing with MALDI-TOF MS - A Novel Method for Rapid Typing of Clostridium difficile.
Clostridium difficile strains were typed by a newly developed MALDI-TOF method, high molecular weight typing, and compared to PCR ribotyping. Among 500 isolates representing 59 PCR ribotypes a total of 35 high molecular weight types could be resolved. Although less discriminatory than PCR ribotyping, the method is extremely fast and simple, and supports for cost-effective screening of isolates during outbreak situations.
Department of Microbiology, Public Health Agency of Sweden, Solna, Sweden.
Kristina
K
Rizzardi
Department of Microbiology, Public Health Agency of Sweden, Solna, Sweden.
Thomas
T
Åkerlund
D015373:Bacterial Typing Techniques / Q000191:economics / Q000379:methods*; D016360:Clostridioides difficile / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D003015:Clostridium Infections / Q000175:diagnosis / Q000382:microbiology*; D006801:Humans; D008970:Molecular Weight; D016133:Polymerase Chain Reaction / Q000379:methods; D021521:Ribotyping / Q000191:economics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000191:economics / Q000379:methods*