The effects of salinity changes on the Polychaete Diopatra neapolitana: Impacts on regenerative capacity and biochemical markers.
Polychaetes have been identified by several authors as a group of marine invertebrates that respond rapidly to anthropogenic stressors. However, studies investigating alterations in Polychaetes affected by climate changes are scarce. Thus, the present study aimed to assess the impact of salinity changes (14, 21, 28, 35, 42g/L) on the physiological and biochemical performance of the Polychaete Diopatra neapolitana, evaluating the species regenerative ability and biochemical alterations. The results obtained demonstrated that organisms exposed to extreme salinity conditions (14, 21 and 42g/L) presented higher mortality rates, needed more days to completely regenerate the missing body region and also regenerated less chaetigers, when compared to organisms exposed to salinities 28 and 35g/L. The present study further demonstrated that D. neapolitana presented significantly lower glycogen and protein content at salinities 21 and 42g/L, which can be explained by higher energy expenditure in the physiological and biochemical processes. A marked impairment of the glutathione redox status was also recorded at salinities 21 and 42g/L. Increased antioxidant enzyme activities were observed at salinity 21g/L while LPO levels were increased at salinity 42g/L. Overall the present study demonstrated that the regenerative capacity of D. neapolitana can be used as a tool to assess environmental changes, namely salinity shifts. Moreover, stress related biomarkers revealed to be useful to evaluate the alterations in Polychaetes due to salinity changes. D. neapolitana revealed to be a good bioindicator to salinity alterations.
Departamento de Biologia & CESAM, Universidade de Aveiro, 3810-193 Aveiro, Portugal. Electronic address: rosafreitas@ua.pt.
Rosa
R
Freitas
Departamento de Biologia & CESAM, Universidade de Aveiro, 3810-193 Aveiro, Portugal.
Adília
A
Pires
Departamento de Biologia & CESAM, Universidade de Aveiro, 3810-193 Aveiro, Portugal.
Cátia
C
Velez
Departamento de Biologia & CESAM, Universidade de Aveiro, 3810-193 Aveiro, Portugal.
Ângela
Â
Almeida
Departamento de Biologia & CESAM, Universidade de Aveiro, 3810-193 Aveiro, Portugal; Department of Geography, University of Victoria, 8500 Finnerty Road, David Turpin Building, Victoria, BC V8P 5C2, Canada.
Frederick J
FJ
Wrona
Departamento de Biologia & CESAM, Universidade de Aveiro, 3810-193 Aveiro, Portugal.
Amadeu M V M
AM
Soares
Departamento de Biologia & CESAM, Universidade de Aveiro, 3810-193 Aveiro, Portugal.
Etelvina
E
Figueira
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D004734:Energy Metabolism; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D011077:Polychaeta / Q000187:drug effects* / Q000378:metabolism; D012038:Regeneration / Q000187:drug effects*; D054712:Salinity; D013482:Superoxide Dismutase / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D000975:Antioxidants; D015415:Biomarkers; D014874:Water Pollutants, Chemical; D002374:Catalase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D005978:Glutathione
Biomarkers; Oxidative stress; Polychaetes; Rainy and drought periods; Redox status