Molecular characterization of outer membrane vesicles released from Acinetobacter radioresistens and their potential roles in pathogenesis.
Acinetobacter radioresistens is an important member of genus Acinetobacter from a clinical point of view. In the present study, we report that a clinical isolate of A. radioresistens releases outer membrane vesicles (OMVs) under in vitro growth conditions. OMVs were released in distinctive size ranges with diameters from 10 to 150 nm as measured by the dynamic light scattering (DLS) technique. Additionally, proteins associated with or present into OMVs were identified using LC-ESI-MS/MS. A total of 71 proteins derived from cytosolic, cell membrane, periplasmic space, outer membrane (OM), extracellular and undetermined locations were found in OMVs. The initial characterization of the OMV proteome revealed a correlation of some proteins to biofilm, quorum sensing, oxidative stress tolerance, and cytotoxicity functions. Thus, the OMVs of A. radioresistens are suggested to play a role in biofilm augmentation and virulence possibly by inducing apoptosis.
Institute of Bioinformatics and Biotechnology, Savitribai Phule Pune University, Pune 411007, India.
Shweta
S
Fulsundar
Centre for Cellular and Molecular Biology, Hyderabad 500007, India.
Heramb M
HM
Kulkarni
Centre for Cellular and Molecular Biology, Hyderabad 500007, India.
Medicharla V
MV
Jagannadham
Institute of Bioinformatics and Biotechnology, Savitribai Phule Pune University, Pune 411007, India.
Rashmi
R
Nair
Centre for Cellular and Molecular Biology, Hyderabad 500007, India.
Sravani
S
Keerthi
Institute of Bioinformatics and Biotechnology, Savitribai Phule Pune University, Pune 411007, India.
Pooja
P
Sant
Department of Microbiology, Savitribai Phule Pune University, Pune 411007, India.
Karishma
K
Pardesi
Department of Chemical Engineering, Indian Institute of Technology Bombay, Mumbai 400076, India.
Jayesh
J
Bellare
Department of Microbiology, Savitribai Phule Pune University, Pune 411007, India; Dr. Babasaheb Ambedkar Marathwada University, Aurangabad 431 004, India. Electronic address: vc@bamu.ac.in.
Balu Ananda
BA
Chopade
D000150:Acinetobacter / Q000472:pathogenicity*; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism; D002853:Chromatography, Liquid; D020543:Proteome / Q000032:analysis*; D022142:Secretory Vesicles / Q000737:chemistry* / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D037521:Virulence Factors / Q000032:analysis*
D001425:Bacterial Outer Membrane Proteins; D020543:Proteome; D037521:Virulence Factors
Acinetobacter radioresistens; Apoptosis; Biofilm augmentation; Cytotoxicity; OMV