Retrospective biodosimetry using translocation frequency in a stable cell of occupationally exposed to ionizing radiation.
Two cases of hematological malignancies were reported in an industrial radiography company over a year, which were reasonably suspected of being consequences of prolonged exposure to ionizing radiation because of the higher incidence than expected in the general population. We analyzed chromosomal aberrations in the peripheral blood lymphocytes from the other workers who had been working under similar circumstances as the patients in the company. Among the subjects tested, 10 workers who belonged to the highest band were followed up periodically for 1.5 years since the first analysis. The aim of this study was to clarify pertinence of translocation analysis to an industrial set-up where chronic exposure was commonly expected. To be a useful tool for a retrospective biodosimetry, the aberrations need to be persistent for a decade or longer. Therefore we calculated the decline rates and half-lives of frequency for both a reciprocal translocation and a dicentric chromosome and compared them. In this study, while the frequency of reciprocal translocations was maintained at the initial level, dicentric chromosomes were decreased to 46.9% (31.0-76.5) of the initial frequency over the follow-up period. Our results support the long-term stability of reciprocal translocation through the cell cycle and validate the usefulness of translocation analysis as a retrospective biodosimetry for cases of occupational exposure.
Department of Emergency Medical Preparedness, National Radiation Emergency Medical Center, Korea Institute of Radiological and Medical Sciences, 75 Nowon-gil, Nowon-gu, Seoul, South Korea Department of Surgery, Yonsei University Wonju College of Medicine, 162 Ilsan-dong, Wonju 220-701, Gangwon-do, South Korea.
Min Su
MS
Cho
Department of Emergency Medical Preparedness, National Radiation Emergency Medical Center, Korea Institute of Radiological and Medical Sciences, 75 Nowon-gil, Nowon-gu, Seoul, South Korea Department of Biological Dosimetry, National Radiation Emergency Medical Center, Korea Institute of Radiological and Medical Sciences, 75 Nowon-gil, Nowon-gu, Seoul, South Korea jklee@kirams.re.kr bksgs@yonsei.ac.kr.
Jin Kyung
JK
Lee
Department of Surgery, Yonsei University Wonju College of Medicine, 162 Ilsan-dong, Wonju 220-701, Gangwon-do, South Korea jklee@kirams.re.kr bksgs@yonsei.ac.kr.
Keum Seok
KS
Bae
Department of Biological Dosimetry, National Radiation Emergency Medical Center, Korea Institute of Radiological and Medical Sciences, 75 Nowon-gil, Nowon-gu, Seoul, South Korea.
Eun-Ae
EA
Han
Department of Biological Dosimetry, National Radiation Emergency Medical Center, Korea Institute of Radiological and Medical Sciences, 75 Nowon-gil, Nowon-gu, Seoul, South Korea.
Seong Jae
SJ
Jang
Department of Health Physics, National Radiation Emergency Medical Center, Korea Institute of Radiological and Medical Sciences, 75 Nowon-gil, Nowon-gu, Seoul, South Korea.
Wi-Ho
WH
Ha
Department of Emergency Medical Preparedness, National Radiation Emergency Medical Center, Korea Institute of Radiological and Medical Sciences, 75 Nowon-gil, Nowon-gu, Seoul, South Korea.
Seung-Sook
SS
Lee
Department of Animal Biology, Plant Biology and Ecology, Autonomous University of Barcelona, 08193 Bellaterra, Catalonia, Spain.
Joan Francesc
JF
Barquinero
Division of Radiation Regulation, Korea Institute of Nuclear Safety, 62 Gwahak-ro, Yuseong-gu, Daejeon 305-338, South Korea.
Wan Tae
WT
Kim
D000328:Adult; D001681:Biological Assay / Q000379:methods*; D015374:Biosensing Techniques / Q000379:methods; D002869:Chromosome Aberrations / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000502:physiology / Q000528:radiation effects; D008297:Male; D016273:Occupational Exposure / Q000032:analysis*; D011829:Radiation Dosage; D000069079:Radiation Exposure / Q000032:analysis*; D011874:Radiometry / Q000379:methods*; D015203:Reproducibility of Results; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D014178:Translocation, Genetic / Q000235:genetics / Q000528:radiation effects*
occupational exposure; retrospective biodosimetry; stable cells; translocation