Registered report: Discovery and preclinical validation of drug indications using compendia of public gene expression data.
The Science Translational Medicine in 2011 (Sirota et al., 2011). The key experiments being replicated include Figure 4C and D and Supplemental Figure 1. In these figures, Sirota and colleagues. tested a proof of concept experiment validating their prediction that cimetidine, a histamine-2 (H2) receptor agonist commonly used to treat peptic ulcers (Kubecova et al., 2011), would be effective against lung adenocarcinoma (Figure 4C and D). As a control they also tested the effects of cimetidine against renal carcinoma, for which it was not predicted to be efficacious (Supplemental Figure 1). The Reproducibility Project: Cancer Biology is a collaboration between the eLife.
Developmental Therapeutics Core, Northwestern University, Evanston, Illinois, United States.
Irawati
I
Kandela
Harvard Medical School, Boston, Massachusetts, United States.
Ioannis
I
Zervantonakis
D000230:Adenocarcinoma / Q000188:drug therapy* / Q000473:pathology; D000077192:Adenocarcinoma of Lung; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D002292:Carcinoma, Renal Cell / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor; D002927:Cimetidine / Q000494:pharmacology*; D004317:Doxorubicin / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical; D058492:Drug Repositioning; D005260:Female; D006635:Histamine H2 Antagonists / Q000494:pharmacology*; D006801:Humans; D007680:Kidney Neoplasms / Q000188:drug therapy* / Q000473:pathology; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000473:pathology; D051379:Mice; D016513:Mice, SCID; D015203:Reproducibility of Results; D016896:Treatment Outcome; D047368:Tumor Burden / Q000187:drug effects; D023041:Xenograft Model Antitumor Assays
D000903:Antibiotics, Antineoplastic; D006635:Histamine H2 Antagonists; D002927:Cimetidine; D004317:Doxorubicin
Reproducibility Project: Cancer Biology; drug retargeting; human; human biology; medicine; methodology