RSRC1 SUMOylation enhances SUMOylation and inhibits transcriptional activity of estrogen receptor β.
The transcription factor estrogen receptor β (ERβ) plays roles in the central nervous, endocrine, cardiovascular, and immune systems. ERβ can be SUMOylated. However, the underlying mechanism remains unclear. Here, we show that RSRC1/SRrp53 interacts with ERβ and SUMOylation of RSRC1 is required for regulation of PIAS1-mediated ERβ SUMOylation. RSRC1 promotes ERβ SUMOylation through enhanced interaction between ERβ and PIAS1. RSRC1 represses ERβ transcriptional activity through regulation of ERβ SUMOylation. By establishing RSRC1 as a novel cofactor for SUMOylation, our data provide insight into regulation of ERβ SUMOylation and indicate that SUMOylation of one protein can regulate another protein SUMOylation.
Department of Medical Molecular Biology, Beijing Institute of Biotechnology, Beijing 100850, PR China.
Lihan
L
Chen
Department of Medical Molecular Biology, Beijing Institute of Biotechnology, Beijing 100850, PR China.
Weini
W
Li
Department of Medical Molecular Biology, Beijing Institute of Biotechnology, Beijing 100850, PR China.
Weiyi
W
Qiu
Department of Medical Molecular Biology, Beijing Institute of Biotechnology, Beijing 100850, PR China.
Wen
W
Ren
Department of Medical Molecular Biology, Beijing Institute of Biotechnology, Beijing 100850, PR China.
Qincao
Q
Li
Department of Medical Molecular Biology, Beijing Institute of Biotechnology, Beijing 100850, PR China.
Baiyu
B
Han
Department of Medical Molecular Biology, Beijing Institute of Biotechnology, Beijing 100850, PR China.
Lei
L
Zhou
Department of Medical Molecular Biology, Beijing Institute of Biotechnology, Beijing 100850, PR China. Electronic address: flonic@163.com.
Long
L
Cheng
Department of Medical Molecular Biology, Beijing Institute of Biotechnology, Beijing 100850, PR China; Cardiovascular Drug Research Center, Tianjin Institute of Health and Environmental Medicine, Tianjin 300050, PR China. Electronic address: zhanghal197@163.com.
Hao
H
Zhang
Department of Medical Molecular Biology, Beijing Institute of Biotechnology, Beijing 100850, PR China. Electronic address: yeqn66@yahoo.com.
Qinong
Q
Ye
D045744:Cell Line, Tumor; D047629:Estrogen Receptor beta / Q000235:genetics / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D015151:Immunoblotting; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D050828:Protein Inhibitors of Activated STAT / Q000235:genetics / Q000378:metabolism; D025941:Protein Interaction Mapping; D058207:Sumoylation; D014158:Transcription, Genetic; D020798:Two-Hybrid System Techniques
D047629:Estrogen Receptor beta; D009687:Nuclear Proteins; D050828:Protein Inhibitors of Activated STAT; C542463:RSRC1 protein, human
ERβ; PIAS1; RSRC1/SRrp53; SUMOylation; Transcriptional activity