Quantification of iodiconazole by LC-MS/MS, a new topical transdermal cream, in dermatophytosis patients' plasma.
BACKGROUND
A rapid and sensitive LC-MS/MS method was established to measure iodiconazole (ADKZ) in dermatophytosis patients treated topically with 2% ADKZ cream.


METHODS
ADKZ was extracted by liquid-liquid extraction (LLE) and separated by an Agilent Zorbax SB-C18 column (2.1 × 150 mm, 3.5 μm) using methanol - 0.01% formic acid (70:30, v/v) as the mobile phase. All the validation assays met the acceptable criteria and the linearity ranged from 10 to 1000 pg/ml.


CONCLUSIONS
The method has been validated to be simple, sensitive and successfully applied to the study. The average amount of ADKZ absorbed into blood was approximately 0.51 μg daily, and ADKZ blood concentrations were consistent during the four-week treatment course. The cumulation of ADKZ in vivo was almost negligible.
MOE Key Laboratory of Drug Quality Control & Pharmacoigilance, China Pharmaceutical University, Nanjing 210009, China.
Hui
H
Zhao
Xiao Dong
XD
She
Xia Xia
XX
Yu
Jin Feng
JF
Fu
Yun
Y
Chen
Yuan
Y
Tian
Zun Jian
ZJ
Zhang
D000287:Administration, Topical; D000328:Adult; D000935:Antifungal Agents / Q000008:administration & dosage* / Q000097:blood* / Q000627:therapeutic use; D001596:Benzylamines / Q000008:administration & dosage* / Q000097:blood* / Q000627:therapeutic use; D001774:Blood Chemical Analysis / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D063465:Skin Cream / Q000737:chemistry*; D053719:Tandem Mass Spectrometry; D014005:Tinea / Q000097:blood* / Q000188:drug therapy; D014230:Triazoles / Q000008:administration & dosage* / Q000097:blood* / Q000627:therapeutic use
C539632:1-(1H-1,2,4-triazole)-2-(2,4-difluorophenyl)-3-(N-methyl-N-(4-iodobenzyl)amino)-2-propanol; D000935:Antifungal Agents; D001596:Benzylamines; D014230:Triazoles