The Cytoprotective Effect of Petalonia binghamiae Methanol Extract against Oxidative Stress in C2C12 Myoblasts: Mediation by Upregulation of Heme Oxygenase-1 and Nuclear Factor-Erythroid 2 Related Factor 2.
This study was designed to examine the protective effects of the marine brown algae Petalonia binghamiae against oxidative stress-induced cellular damage and to elucidate the underlying mechanisms. P. binghamiae methanol extract (PBME) prevented hydrogen peroxide (H2O2)-induced growth inhibition and exhibited scavenging activity against intracellular reactive oxygen species (ROS) induced by H2O2 in mouse-derived C2C12 myoblasts. PBME also significantly attenuated H2O2-induced comet tail formation in a comet assay, histone γH2A.X phosphorylation, and annexin V-positive cells, suggesting that PBME prevented H2O2-induced cellular DNA damage and apoptotic cell death. Furthermore, PBME increased the levels of heme oxygenase-1 (HO-1), a potent antioxidant enzyme, associated with the induction of nuclear factor-erythroid 2 related factor 2 (Nrf2). However, zinc protoporphyrin IX, a HO-1 competitive inhibitor, significantly abolished the protective effects of PBME on H2O2-induced ROS generation, growth inhibition, and apoptosis. Collectively, these results demonstrate that PBME augments the antioxidant defense capacity through activation of the Nrf2/HO-1 pathway.
Blue-Bio Industry RIC and Anti-Aging Research Center, Dongeui University, Busan 614-714, Korea. 13839@deu.ac.kr.
Ji Sook
JS
Kang
Department of Microbiology, College of Medicine, Inje University, Busan 608-756, Korea. cihima@inje.ac.kr.
Il-Whan
IW
Choi
Marine Biodiversity Institute of Korea, Seocheon 325-902, Korea. alsgh0615@lycos.co.kr.
Min Ho
MH
Han
Marine Biodiversity Institute of Korea, Seocheon 325-902, Korea. lds8270@hanmail.net.
Dae-Sung
DS
Lee
Laboratory of Immunobiology, Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Korea. immunkim@cheju.ac.kr.
Gi-Young
GY
Kim
Blue-Bio Industry RIC and Anti-Aging Research Center, Dongeui University, Busan 614-714, Korea. lab301@nate.com.|Department of Food and Nutrition, College of Natural Sciences & Human Ecology, Dongeui University, Busan 614-714, Korea. lab301@nate.com.
Hye Jin
HJ
Hwang
Blue-Bio Industry RIC and Anti-Aging Research Center, Dongeui University, Busan 614-714, Korea. bwkim@deu.ac.kr.|Department of Life Science and Biotechnology, College of Natural Sciences & Human Ecology, Dongeui University, Busan 614-714, Korea. bwkim@deu.ac.kr.
Byung Woo
BW
Kim
Department of Biochemistry, Busan National University College of Medicine, Yangsan 626-870, Korea. kimcm@pusan.ac.kr.
Cheol Min
CM
Kim
Department of Anatomy and Cell Biology, Dong-A University College of Medicine & Mitochondria Hub Regulation Center, Busan 602-714, Korea. yhyoo@dau.ac.kr.
Young Hyun
YH
Yoo
Blue-Bio Industry RIC and Anti-Aging Research Center, Dongeui University, Busan 614-714, Korea. choiyh@deu.ac.kr.|Department of Biochemistry, Dongeui University College of Korean Medicine, Busan 614-052, Korea. choiyh@deu.ac.kr.
Yung Hyun
YH
Choi
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D002478:Cells, Cultured; D004249:DNA Damage / Q000187:drug effects; D051547:Heme Oxygenase-1 / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D000432:Methanol / Q000737:chemistry; D051379:Mice; D032446:Myoblasts / Q000187:drug effects* / Q000378:metabolism; D051267:NF-E2-Related Factor 2 / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D000459:Phaeophyceae / Q000737:chemistry*; D010766:Phosphorylation / Q000187:drug effects; D020011:Protective Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects*
D000975:Antioxidants; D051267:NF-E2-Related Factor 2; D020011:Protective Agents; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D051547:Heme Oxygenase-1; D000432:Methanol