Differential expressions of cancer-associated genes and their regulatory miRNAs in colorectal carcinoma.
Colorectal cancer is one of the frequently seen malignancies in the world. To date, several oncogenes and tumor suppressor genes have been identified and linked to colorectal cancer pathogenesis. Although recent advances in the diagnosis and therapy of colorectal cancer are promising, identifying novel genetic contributors is still high priority. In the present study, expression profile of some cancer-related genes and their regulatory miRNA molecules were evaluated by using a high-throughput real-time PCR method. For the study, a total of 54 patients diagnosed with CRC and normal colon tissue samples of 42 healthy controls were included. For the expression analysis, total RNA was extracted from FFPE tissue samples and converted to cDNA. All expression analyses were assessed by using Fluidigm Microfluidic Dynamic Array chips for 96 samples and the reactions were held in Fluidigm BioMark™ HD System Real-Time PCR. As a result of the study, expression of the ADAMTS1, FHIT, RUNX1, RUNX3 and WWOX genes was shown to be significantly altered in CRC tissues in contrast to normal tissue samples. Moreover, miR-378a-3p, miR-155-5p, miR-193b-3p, miR-96-5p, miR-17-5p, miR-27a-3p, miR-133b, miR-203a, miR-205-5p, miR-34c-5p, miR-130a-3p, miR-301a-3p, miR-132-3p, miR-222-3p, miR-34a-5p, miR-21-5p, miR-29a-3p and miR-29b-3p were found to be significantly deregulated in CRC. Consequently, results of the current study strongly suggest the involvement of novel cancer-related genes and their regulatory miRNAs in CRC physiopathology.
Department of Medical Genetics, Faculty of Medicine, Mugla Sıtkı Kocman University, Mugla, Turkey.
Murat
M
Kara
Department of Medical Biology, Faculty of Medicine, Adiyaman University, Adiyaman, Turkey.
Onder
O
Yumrutas
Department of General Surgery, Faculty of Medicine, Mugla Sıtkı Kocman University, Mugla, Turkey.
Onder
O
Ozcan
Department of Medical Pathology, Faculty of Medicine, Mugla Sıtkı Kocman University, Mugla, Turkey.
Ozgur Ilhan
OI
Celik
Department of Medical Biology and Genetics, Faculty of Medicine, University of Gaziantep, Gaziantep, Turkey.
Esra
E
Bozgeyik
Department of Medical Biology, Faculty of Medicine, Adiyaman University, Adiyaman, Turkey. Electronic address: i.bozgeyik@gmail.com.
Ibrahim
I
Bozgeyik
Department of Medical Genetics, Faculty of Medicine, Ataturk University, Erzurum, Turkey.
Sener
S
Tasdemir
D015179:Colorectal Neoplasms / Q000235:genetics*; D052138:Genes, Neoplasm; D020022:Genetic Predisposition to Disease; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D044085:Microfluidics; D060888:Real-Time Polymerase Chain Reaction
D035683:MicroRNAs
ADAMTS1; Colorectal cancer; FHIT; RUNXs; SIRT1; WWOX; miRNA