Independent and combined influence of the FTO rs9939609 and MC4Rrs17782313 polymorphisms on hypocaloric diet induced changes in body mass and composition and energy metabolism in non-morbid obese premenopausal women.
OBJECTIVE
To examine the independent and combined influence of the FTOrs9939609 and the MC4Rrs17782313 polymorphisms on changes in fat mass (FM), resting energy expenditure (REE), leptin, and thyrotropin (TSH) levels, after a 12-week energy-restricted diet intervention in non-morbid premenopausal obese women.


METHODS
Fat mass (dual X-ray absorptiometry), REE (indirect calorimetry) and plasma leptin and thyrotropin levels were measured (before and after the intervention) in 77 obese (BMI: 33.9 ± 2.8 kg/m(2)) women (age: 36.8 ± 7.0y).


RESULTS
There were no significant differences across FTOrs9939609 genotype groups (TT vs. A allele carriers, Ps>0.1) on changes in body mass (-8.6 ± 3.2% vs. -8.7 ± 3.3 %), FM (12.8 ± 4.7% vs. -12.9 ± 6.3%), REE (-11.3 ± 4.7 vs. -9.4 ± 8.1%), leptin (-34.1 ± 25.1% vs. -43.5 ± 24.1%) or TSH (5.2 ± 34.5% vs. -1.7 ± 27.1%) levels. Moreover, it was not observed any significant difference on changes in body mass (-8.6 ± 3.6% vs. -8.9 ± 2.6%), FM (-12.7 ± 6.1% vs. -13.4 ± 5.3%), REE (-9.8 ± 7.4% -9.4 ± 9.4%), leptin (-39.0 ± 26.9% vs. -44.8 ± 18.4%) or TSH (-1.0 ± 30.0% vs. 1.5 ± 26.5%) levels between non-C allele carriers and C allele carriers of the MC4Rrs17782313 (Ps>0.3). Finally, there were no significant difference on changes in body mass and composition, REE, leptin or TSH levels among non-risk allele carriers, carriers of the C allele risk of the MC4Rrs17782313, carriers of the A allele of the FTOrs9939609 and carriers of both risk alleles after the 12-week energy-restricted diet intervention (Ps>0.1).


CONCLUSIONS
Carrying the A risk allele of the FTOrs9939609 and/or the C risk allele of the MC4Rrs17782313 did not influence body mass and FM loss, or REE decrease in obese women after a 12-week energy-restricted diet intervention.
Department of Nutrition and Food Science, University of the Basque Country, UPV/EHU, Vitoria, Spain. Elikadura, Ariketa fisikoa eta Osasuna, ELIKOS, Nutrition, Exercise and Health, Research group, University of the Basque Country, UPV/EHU, Vitoria, Spain.. idoia.labayen@ehu.es.
Idoia
I
Labayen
Elikadura, Ariketa fisikoa eta Osasuna, ELIKOS, Nutrition, Exercise and Health, Research group, University of the Basque Country, UPV/EHU, Vitoria, Spain. Biomedical Research Area, TECNALIA, Miñano, Spain.. idoia.labayen@ehu.es.
Javier
J
Margareto
Elikadura, Ariketa fisikoa eta Osasuna, ELIKOS, Nutrition, Exercise and Health, Research group, University of the Basque Country, UPV/EHU, Vitoria, Spain. Department of Physical Education and Sport, University of the Basque Country, UPV/EHU, Vitoria, Spain.. idoia.labayen@ehu.es.
Sara
S
Maldonado-Martin
Elikadura, Ariketa fisikoa eta Osasuna, ELIKOS, Nutrition, Exercise and Health, Research group, University of the Basque Country, UPV/EHU, Vitoria, Spain. Department of Physical Education and Sport, University of the Basque Country, UPV/EHU, Vitoria, Spain.. idoia.labayen@ehu.es.
Ilargi
I
Gorostegi
Department of Nutrition and Food Science, University of the Basque Country, UPV/EHU, Vitoria, Spain. Elikadura, Ariketa fisikoa eta Osasuna, ELIKOS, Nutrition, Exercise and Health, Research group, University of the Basque Country, UPV/EHU, Vitoria, Spain.. idoia.labayen@ehu.es.
Maitane
M
Illera
Department of Nutrition and Food Science, University of the Basque Country, UPV/EHU, Vitoria, Spain. Elikadura, Ariketa fisikoa eta Osasuna, ELIKOS, Nutrition, Exercise and Health, Research group, University of the Basque Country, UPV/EHU, Vitoria, Spain.. idoia.labayen@ehu.es.
María
M
Medrano
Elikadura, Ariketa fisikoa eta Osasuna, ELIKOS, Nutrition, Exercise and Health, Research group, University of the Basque Country, UPV/EHU, Vitoria, Spain. Departament of Medicine, University of the Basque Country, UPV/EHU, Vitoria, Spain.. idoia.labayen@ehu.es.
Lurdes
L
Barrenechea
Elikadura, Ariketa fisikoa eta Osasuna, ELIKOS, Nutrition, Exercise and Health, Research group, University of the Basque Country, UPV/EHU, Vitoria, Spain. Biomedical Research Area, TECNALIA, Miñano, Spain.. idoia.labayen@ehu.es.
Eider
E
Larrarte
D000328:Adult; D000071516:Alpha-Ketoglutarate-Dependent Dioxygenase FTO / Q000235:genetics*; D001823:Body Composition; D001835:Body Weight; D004038:Diet, Reducing; D004734:Energy Metabolism / Q000235:genetics; D005260:Female; D006801:Humans; D008875:Middle Aged; D009765:Obesity / Q000178:diet therapy* / Q000235:genetics*; D011110:Polymorphism, Genetic; D017698:Postmenopause; D044105:Receptor, Melanocortin, Type 4 / Q000235:genetics*; D055815:Young Adult
C470072:MC4R protein, human; D044105:Receptor, Melanocortin, Type 4; D000071516:Alpha-Ketoglutarate-Dependent Dioxygenase FTO; C526492:FTO protein, human