Drosophila melanogaster Hedgehog cooperates with Frazzled to guide axons through a non-canonical signalling pathway.
We report that the morphogen Hedgehog (Hh) is an axonal chemoattractant in the midline of Drosophila melanogaster embryos. Hh is present in the ventral nerve cord during axonal guidance and overexpression of hh in the midline causes ectopic midline crossing of FasII-positive axonal tracts. In addition, we show that Hh influences axonal guidance via a non-canonical signalling pathway dependent on Ptc. Our results reveal that the Hh pathway cooperates with the Netrin/Frazzled pathway to guide axons through the midline in invertebrates.
Institut de Biologia Molecular de Barcelona (IBMB-CSIC), Parc Cientific de Barcelona, C. Baldiri Reixac 10, Barcelona 08028, Spain; Institut de Recerca Biomedica de Barcelona (IRB), Parc Cientific de Barcelona, C. Baldiri Reixac 10, Barcelona 08028, Spain.
Delia
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Ricolo
Institut de Biologia Molecular de Barcelona (IBMB-CSIC), Parc Cientific de Barcelona, C. Baldiri Reixac 10, Barcelona 08028, Spain; Institut de Recerca Biomedica de Barcelona (IRB), Parc Cientific de Barcelona, C. Baldiri Reixac 10, Barcelona 08028, Spain.
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Butí
Institut de Biologia Molecular de Barcelona (IBMB-CSIC), Parc Cientific de Barcelona, C. Baldiri Reixac 10, Barcelona 08028, Spain; Institut de Recerca Biomedica de Barcelona (IRB), Parc Cientific de Barcelona, C. Baldiri Reixac 10, Barcelona 08028, Spain. Electronic address: sarbmc@ibmb.csic.es.
Sofia J
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Araújo
D000818:Animals; D001369:Axons / Q000378:metabolism* / Q000502:physiology; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000378:metabolism* / Q000502:physiology; D006364:Hedgehogs / Q000378:metabolism*; D009414:Nerve Growth Factors / Q000378:metabolism; D000075386:Netrin Receptors; D055495:Neurogenesis / Q000502:physiology; D011956:Receptors, Cell Surface / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*
D029721:Drosophila Proteins; D009414:Nerve Growth Factors; D000075386:Netrin Receptors; D011956:Receptors, Cell Surface; C000617310:fra protein, Drosophila
Axon; DCC; Drosophila; Frazzled; Hedgehog; Patched