Development and validation of an ESI-LC-MS/MS method for simultaneous identification and quantification of 24 analytes of forensic relevance in vitreous humour, whole blood and plasma.
Detection and quantification of drugs from various biological matrices are of immense importance in forensic toxicological analysis. Despite the various reported methods, development of a new method for the detection and quantification of drugs is still an active area of research. However, every method and biological matrix has its own limitation, which further encourage forensic toxicologists to develop new methods and to explore new matrices for the analysis of drugs. In this study, an electrospray ionization-liquid chromatograph-tandem mass spectrometry (ESI-LC-MS/MS) method is developed and validated for simultaneous identification and quantification of 24 drugs of forensic relevance in various body fluids, namely, whole blood, plasma and vitreous humour. The newly developed method has been validated for intra-day and inter-day accuracy, precision, selectivity and sensitivity. Absolute recovery shows a mean of 84.5, 86.2, and 103% in the vitreous humour, whole blood and plasma respectively, which is suitable for the screening procedure. Further, the absolute matrix effect (AME) shows a mean of 105, 96.5, and 109% in the vitreous humour, whole blood and plasma, respectively. In addition, to examine the practical utility of this method, it has been applied for screening of drugs in post-mortem samples of the vitreous humour, whole blood and plasma collected at autopsy from ten cadavers. Experimental results show that the newly developed method is well applicable for screening of analytes in all the three matrices.
Department of Ophthalmology, Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, 110029, India.|Department of Forensic Science, Institute of Forensic Science, Mumbai, Maharashtra, 400032, India.
Beauty
B
Arora
Department of Ocular Pharmacology and Pharmacy, Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, 110029, India.
Thirumurthy
T
Velpandian
Department of Ophthalmology, Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, 110029, India.
Rohit
R
Saxena
Department of Forensic Medicine and Toxicology, All India Institute of Medical Sciences, New Delhi, 110029, India.
Sanjeev
S
Lalwani
Department of Forensic Medicine and Toxicology, All India Institute of Medical Sciences, New Delhi, 110029, India.
T D
TD
Dogra
Department of Ophthalmology, Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, 110029, India.
Supriyo
S
Ghose
D001344:Autopsy; D002853:Chromatography, Liquid / Q000379:methods; D053593:Forensic Toxicology / Q000379:methods; D006801:Humans; D057230:Limit of Detection; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000097:blood*; D010949:Plasma / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014822:Vitreous Body / Q000737:chemistry*
D004364:Pharmaceutical Preparations
ESI-LC-MS/MS; drugs, forensic; post-mortem; vitreous humour