Sensitivity improvement of kukoamine determination by complexation with dihydrogen phosphate anions in capillary zone electrophoresis.
A novel complexation between kukoamines and dihydrogen phosphate ions (DPI) during CZE was discovered to improve the UV signal of kukoamine by around 30-fold. This complexation formed by electric current was attributed to the hydrogen bonding of hydroxyl and amino (or amide) groups between the analyte and electrolyte anions. The established CZE method is low-cost, easy to operate, and eco-friendly, and it was shown to be superior to HPLC in terms of separation capability, efficiency, specificity, and sensitivity. We believe that our CZE method can be applied as an alternative to HPLC for kukoamine assay. The approach described here can be also extended for analyzing other compounds with similar functional groups.
Department of Biomedical Sciences, Research Group for Bioactive Products, City University of Hong Kong, Hong Kong SAR, P. R., China.
Yuan-Yuan
YY
Li
Department of Biomedical Sciences, Research Group for Bioactive Products, City University of Hong Kong, Hong Kong SAR, P. R., China.
Rui
R
Di
Department of Biomedical Sciences, Research Group for Bioactive Products, City University of Hong Kong, Hong Kong SAR, P. R., China.
Wing-Leung
WL
Hsu
Department of Biomedical Sciences, Research Group for Bioactive Products, City University of Hong Kong, Hong Kong SAR, P. R., China.
Ye-Qing
YQ
Huang
Key laboratory of Biochip Technology, Shenzhen Biotech and Health Centre, City University of Hong Kong, Shenzhen, P. R., China.|Department of Biology and Chemistry, City University of Hong Kong, Hong Kong, P. R., China.
Hongyan
H
Sun
Department of Biomedical Sciences, Research Group for Bioactive Products, City University of Hong Kong, Hong Kong SAR, P. R., China.|Key laboratory of Biochip Technology, Shenzhen Biotech and Health Centre, City University of Hong Kong, Shenzhen, P. R., China.
Hon-Yeung
HY
Cheung
D000838:Anions / Q000737:chemistry*; D002109:Caffeic Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary / Q000379:methods*; D006860:Hydrogen Bonding; D016014:Linear Models; D010710:Phosphates / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019657:Solanaceae / Q000737:chemistry; D013096:Spermine / Q000031:analogs & derivatives* / Q000032:analysis
D000838:Anions; D002109:Caffeic Acids; D010710:Phosphates; C096274:kukoamine A; C557784:kukoamine B; D013096:Spermine
Capillary zone electrophoresis; Complexation; Dihydrogen phosphate ion; Kukoamines; Sensitivity