ToF-SIMS imaging of lipids and lipid related compounds in Drosophila brain.
Drosophila melanogaster(fruit fly) has a relatively simple nervous system but possesses high order brain functions similar to humans. Therefore, it has been used as a common model system in biological studies, particularly drug addiction. Here, the spatial distribution of biomolecules in the brain of the fly was studied using time-of flight secondary ion mass spectrometry (ToF-SIMS). Fly brains were analyzed frozen to prevent molecular redistribution prior to analysis. Different molecules were found to distribute differently in the tissue, particularly the eye pigments, diacylglycerides, and phospholipids, and this is expected to be driven by their biological functions in the brain. Correlations in the localization of these molecules were also observed using principal components analysis of image data, and this was used to identify peaks for further analysis. Furthermore, consecutive analyses following 10 keV Ar2500+sputtering showed that different biomolecules respond differently to Ar2500+sputtering. Significant changes in signal intensities between consecutive analyses were observed for high mass molecules including lipids.
Department of Chemistry and Molecular Biology, University of Gothenburg, Kemivägen 10, SE-412 96 Gothenburg, Sweden ; National Center Imaging Mass Spectrometry, Kemivägen 10, SE-412 96 Gothenburg, Sweden.
Nhu T N
NT
Phan
Department of Chemistry and Molecular Biology, University of Gothenburg, Kemivägen 10, SE-412 96 Gothenburg, Sweden ; National Center Imaging Mass Spectrometry, Kemivägen 10, SE-412 96 Gothenburg, Sweden.
John S
JS
Fletcher
Department of Chemistry and Molecular Biology, University of Gothenburg, Kemivägen 10, SE-412 96 Gothenburg, Sweden ; Chemistry, Materials and Surfaces, SP Technical Research Institute of Sweden, SE-50115 Borås, Sweden.
Peter
P
Sjövall
Department of Chemistry and Molecular Biology, University of Gothenburg, Kemivägen 10, SE-412 96 Gothenburg, Sweden ; National Center Imaging Mass Spectrometry, Kemivägen 10, SE-412 96 Gothenburg, Sweden ; Department of Chemical and Biological Engineering, Chalmers University of Technology, Kemivägen 10, SE-412 96 Gothenburg, Sweden.
Andrew G
AG
Ewing
Drosophila; SIMS; imaging mass spectrometry; lipids