The proliferative and migratory effects of physical injury and stromal cell-derived factor-1α on rat cardiomyocytes and fibroblasts.
OBJECTIVE
This study aims to explore the effects of physical injury and stromal cell-derived factor-1α (SDF-1α) on the proliferation of cardiomyocytes and chemotactic effects of cardiomyocytes on the migration of cardiac fibroblasts.


METHODS
Isolation and primary culture of rat cardiomyocytes and cardiac fibroblasts were performed; scratching was employed to induce physical injury on cells which were cultured with SDF-1α at different concentrations; proliferation ability of cardiomyocytes was checked with CCK-8 assay and migratory ability of cardiac fibroblasts under the chemotaxis of cardiomyocytes was detected with Transwell assay.


RESULTS
SDF-1α enhanced the proliferation ability of cardiomyocytes with physical injury, especially at the concentration of 80 µg/L when the proliferation rate of cardiomyocytes increased most markedly. Moreover, physically injured cardiomyocyte that was cultured with SDF-1α significantly elevated migratory ability of cardiac fibroblasts, which tended to be more obvious along with the chemotactic culture time.


CONCLUSIONS
SDF-1α enhanced the proliferation ability of cardiomyocytes with physical injury, and physically injured cardiomyocyte that was cultured with SDF-1α promoted the migration of cardiac fibroblasts.
Department of Congenital Heart Disease, Cardiovascular Research Institute of PLA, General Hospital of Shenyang Military Command, Shenyang, Liaoning Province, China. chuanjuhou@126.com.
C-J
CJ
Hou
Y-M
YM
Qi
D-Z
DZ
Zhang
Q-G
QG
Wang
C-S
CS
Cui
L
L
Kuang
B
B
Chen
D000818:Animals; D000831:Animals, Newborn; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology*; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D054377:Chemokine CXCL12