A comprehensive study on algal-bacterial communities shift during thiocyanate degradation in a microalga-mediated process.
Changes in algal and bacterial communities during thiocyanate (SCN(-)) decomposition in a microalga-mediated process were studied. Pyrosequencing indicated that Thiobacillus bacteria and Micractinium algae predominated during SCN(-) hydrolysis, even after its complete degradation. Principal components analysis and evenness profiles (based on the Pareto-Lorenz curve) suggested that the changes in the bacterial communities were driven by nitrogen and sulfur oxidation, pH changes, and photoautotrophic conditions. The populations of predominant microalgae remained relatively stable during SCN(-) hydrolysis, but the proportion of bacteria - especially nitrifying bacteria - fluctuated. Thus, the initial microalgal population may be crucial in determining which microorganisms dominate when the preferred nitrogen source becomes limited. The results also demonstrated that microalgae and SCN(-)-hydrolyzing bacteria can coexist, that microalgae can be effectively used with these bacteria to completely treat SCN(-), and that the structure of the algal-bacterial community is more stable than the community of nitrifying bacteria alone during SCN(-) degradation.
Environmental and Energy Program, KAIST, 291 Daehak-ro, Yuseong, Daejeon, Republic of Korea; Decontamination and Decommissioning Research Division, Korea Atomic Energy Research Institute (KAERI), 989-111 Daedukdaero, Yuseong-gu, Daejeon, Republic of Korea.
Byung-Gon
BG
Ryu
Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon, Republic of Korea.
Woong
W
Kim
Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon, Republic of Korea.
Kibok
K
Nam
Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon, Republic of Korea.
Sungwhan
S
Kim
Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon, Republic of Korea.
Bongsoo
B
Lee
Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon, Republic of Korea.
Min S
MS
Park
Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon, Republic of Korea; Advanced Biomass R&D Center, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon, Republic of Korea. Electronic address: jwyang@kaist.ac.kr.
Ji-Won
JW
Yang
D018407:Bacterial Physiological Phenomena; D016000:Cluster Analysis; D058086:Microalgae / Q000378:metabolism* / Q000502:physiology; D009584:Nitrogen / Q000378:metabolism; D013861:Thiocyanates / Q000378:metabolism*
D013861:Thiocyanates; D009584:Nitrogen
Algal community; Bacterial community; Microbial dynamics; Pyrosequencing; Thiocyanate (SCN(−))