Evaluation of rapid methods for diagnosis of tuberculosis in slaughtered free-range pigs.
Free-range pigs can be infected by Mycobacterium tuberculosis complex (MTC) and may contribute to the spread of bovine tuberculosis (bTB). In the present study, the diagnostic values of bacteriological culture, a duplex real-time quantitative PCR and an antibody ELISA were evaluated in an abattoir study of submandibular lymph nodes and serum samples from 73 pigs with and without lesions consistent with bTB. The duplex qPCR was an accurate method for diagnosis of TB in pigs (specificity 100%; sensitivity 80%). Combining qPCR with histopathology improved sensitivity and had very good concordance (κ = 0.94) with the reference method. Serological results suggest that the antibody ELISA can be used for monitoring herds but not individuals.
Department of Animal Health, University of Córdoba, International Excellence Agrifood Campus 'CeiA3', University Campus of Rabanales, Córdoba 14071, Spain; CICAP - Food Research Centre, Pozoblanco 14400, Córdoba, Spain. Electronic address: fcardoso@cicap.es.
Fernando
F
Cardoso-Toset
Department of Animal Health, University of Córdoba, International Excellence Agrifood Campus 'CeiA3', University Campus of Rabanales, Córdoba 14071, Spain.
Inmaculada
I
Luque
Department of Anatomy and Comparative Pathology, University of Córdoba, International Excellence Agrifood Campus 'CeiA3', University Campus of Rabanales, Córdoba 14071, Spain.
Shyrley Paola
SP
Amarilla
Department of Animal Health, University of Córdoba, International Excellence Agrifood Campus 'CeiA3', University Campus of Rabanales, Córdoba 14071, Spain.
Lidia
L
Gómez-Gascón
CICAP - Food Research Centre, Pozoblanco 14400, Córdoba, Spain.
Layla
L
Fernández
Department of Animal Health, University of Córdoba, International Excellence Agrifood Campus 'CeiA3', University Campus of Rabanales, Córdoba 14071, Spain.
Belén
B
Huerta
Department of Anatomy and Comparative Pathology, University of Córdoba, International Excellence Agrifood Campus 'CeiA3', University Campus of Rabanales, Córdoba 14071, Spain.
Librado
L
Carrasco
Microbiology Service, Reina Sofia University Hospital, Mycobacteria Reference Center, Avd. Méndez Pidal s/n, Córdoba 14004, Spain.
Pilar
P
Ruiz
CICAP - Food Research Centre, Pozoblanco 14400, Córdoba, Spain.
Jaime
J
Gómez-Laguna
D000818:Animals; D001431:Bacteriological Techniques / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000473:pathology; D013997:Time Factors; D014376:Tuberculosis / Q000175:diagnosis / Q000662:veterinary*
Diagnosis; ELISA; Pigs; Real-time quantitative PCR; Tuberculosis