Micronucleus test in peripheral blood of rats treated with hyperbaric oxygen after subtotal splenectomy preserving the lower pole.
OBJECTIVE
To assess the mutagenic potential of the oxygen inhalation therapy (HBO), by means of the micronucleus test, performed in peripheral blood of rats that underwent subtotal splenectomy with lower pole preservation (ESTPI), after HBO sessions or simulations.


METHODS
Eighteen male Wistar rats, were distributed into three groups of six animals: group 1 - submitted to ESTPI and HBO sessions; group 2 - submitted to ESTPI and HBO simulations; group 3 - underwent cyclophosphamide administration. In groups 1 and 2, blood samples from the animals' tails were collected before surgery (T0) and immediately after the 13th HBO session or simulation (T1). In group 3, tail blood samples were collected from animals before (T0) and 24 hours after (T1) cyclophosphamide (CP) delivery. The number of micronucleated normochromatic erythrocytes (MNNCE) was determined by blind counting 2000 normochromatic erythrocytes (NCE) per animal.


RESULTS
Micronuclei average after CP delivery in group 3 was higher than before its use, thus confirming the mutagenic activity of this drug (p=0.01). In groups 1 and 2, no significant difference in the average of Micronuclei was observed when comparing it to blood samples before and after the 13th HBO session or simulation.


CONCLUSIONS
The treatment protocol used in this study did not induce Micronucleus formation in animals submitted to ESTPI and HBO treatment or simulation.
UFMG, Belo Horizonte, MG, Brazil.
Marcela Souza Lima
MS
Paulo
EMESCAM, Vitoria, ES, Brazil.
Ingryd Fortes
IF
Souza
EMESCAM, Vitoria, ES, Brazil.
Kethleen Gomes
KG
Wandekoken
EMESCAM, Vitoria, ES, Brazil.
Karina Balestreiro
KB
Silva
UFES, Vitoria, ES, Brazil.
Jean Carlos Vencioneck
JC
Dutra
UFES, Vitoria, ES, Brazil.
Josivany Valério de
JV
Freitas
UFES, Vitoria, ES, Brazil.
Nataly Pescinalli
NP
Stegmiller
EMESCAM, Vitoria, ES, Brazil.
Lúcia Helena Sagrillo
LH
Pimassoni
UFES, Vitoria, ES, Brazil.
Maria do Carmo Pimentel
Mdo C
Batitucci
EMESCAM, Vitoria, ES, Brazil.
Danilo Nagib Salomão
DN
Paulo
EMESCAM, Vitoria, ES, Brazil.
Flávia Imbroisi Valle
FI
Errera
D000818:Animals; D003520:Cyclophosphamide / Q000494:pharmacology; D006931:Hyperbaric Oxygenation / Q000379:methods*; D008297:Male; D015162:Micronucleus Tests; D009152:Mutagenicity Tests; D009153:Mutagens / Q000494:pharmacology; D011184:Postoperative Period; D017208:Rats, Wistar; D013154:Spleen / Q000601:surgery*; D013156:Splenectomy / Q000379:methods*; D013997:Time Factors; D016896:Treatment Outcome
D009153:Mutagens; D003520:Cyclophosphamide