Plasmon waveguide resonance for sensing glycan-lectin interactions.
Carbohydrate-modified interfaces have been shown to be valuable tools for the study of protein-glycan recognition events. Label-free approache such as plasmonic based techniques are particularly attractive. This paper describes a new analytical platform for the sensitive and selective screening of carbohydrate-lectin interactions using plasmon waveguide resonance. Planar optical waveguides (POW), consisting of glass prisms coated with silver (50 nm) and silica (460 nm) layers were derivatized with mannose or lactose moieties. The specific association of the resulting interface with selected lectins was assessed by following the changes in its plasmonic response. The immobilization strategy investigated in this work is based on the formation of a covalent bond between propargyl-functionalized glycans and surface-linked azide groups via a Cu(I) "click" chemistry. Optimization of the surface architecture through the introduction of an oligo(ethylene glycol) spacer between the plasmonic surface and the glycan ligands provided an interface which allowed screening of glycan-lectin interactions in a highly selective manner. The limit of detection (LOD) of this method for this particular application was found to be in the subnanomolar range (0.5 nM), showing it to constitute a promising analytical platform for future development and use in a pharmaceutical or biomedical setting.
CBMN, UMR 5248 CNRS, Université de Bordeaux, Bat B14, allée Geoffroy St. Hilaire, Pessac, France.
Isabel
I
Alves
Institute of Electronics, Microelectronics and Nanotechnology (IEMN),UMR-CNRS 8520, Université Lille 1, Cité Scientifique, 59655 Villeneuve d'Ascq, France; Taras Shevchenko University, 60 Vladimirskaya Str., Kiev, Ukraine.
Ievgen
I
Kurylo
Institute of Electronics, Microelectronics and Nanotechnology (IEMN),UMR-CNRS 8520, Université Lille 1, Cité Scientifique, 59655 Villeneuve d'Ascq, France.
Yannick
Y
Coffinier
Laboratoire des Glycochimie, des Antimicrobiens et des Agroressources (FRE 3517 CNRS), Université de Picardie Jules Vernes, 33 Rue Saint Leu, 80039 Amiens, France.
Aloysius
A
Siriwardena
Taras Shevchenko University, 60 Vladimirskaya Str., Kiev, Ukraine; Chemistry Department, Pontifical Catholic University of Rio de Janeiro, Rua Marques de Sao Vicente, 225-Gavea, Rio de Janeiro 22451-900, Brazil.
Vladimir
V
Zaitsev
CBMN, UMR 5248 CNRS, Université de Bordeaux, Bat B14, allée Geoffroy St. Hilaire, Pessac, France.
Etienne
E
Harté
Institute of Electronics, Microelectronics and Nanotechnology (IEMN),UMR-CNRS 8520, Université Lille 1, Cité Scientifique, 59655 Villeneuve d'Ascq, France.
Rabah
R
Boukherroub
Institute of Electronics, Microelectronics and Nanotechnology (IEMN),UMR-CNRS 8520, Université Lille 1, Cité Scientifique, 59655 Villeneuve d'Ascq, France. Electronic address: sabine.szunerits@iri.univ-lille1.fr.
Sabine
S
Szunerits
D010367:Arachis / Q000737:chemistry / Q000378:metabolism*; D001386:Azides / Q000737:chemistry; D057930:Click Chemistry; D037102:Lectins / Q000032:analysis / Q000378:metabolism*; D027806:Lens Plant / Q000737:chemistry / Q000378:metabolism*; D008358:Mannose / Q000737:chemistry / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D012822:Silicon Dioxide / Q000737:chemistry; D020349:Surface Plasmon Resonance / Q000379:methods*; D013499:Surface Properties
D001386:Azides; D037102:Lectins; D011134:Polysaccharides; D012822:Silicon Dioxide; D008358:Mannose
Glycan chip; Planar optical waveguide; Plasmon waveguide resonance; “Click” chemistry