First chitin extraction from Plumatella repens (Bryozoa) with comparison to chitins of insect and fungal origin.
Chitin immediately suggests the representatives of the kingdom Fungi, as well as such phyla as Annelida, Mollusca, Porifera, Cnidaria and, mostly, Arthropoda. Although Bryozoa also represents a chitin-containing phylum, no study has been developed yet on the isolation or characterization of the chitin from it. In this study, physiochemical properties of the chitin isolated from Plumatella repens belonging to the phylum Bryozoa was determined for the first time. The chitin structure was also studied comparatively by isolating chitin from an insect species (Palomena prasina) of the phylum Arthropoda, and Fomes fomentarius belonging to the kingdom Fungi. It was observed that the bryozoan chitin was in the α form, as in the arthropod and fungal chitins. The chitin contents in the dry weight of the bryozoan, fungal and insect species were observed to be 13.3%, 2.4%, and 10.8%, respectively. The insect chitin exhibited the highest thermal stability followed by that of the bryozoan and then the fungal chitins. Surface morphologies reveal that the insect and bryozoan chitins were composed of nano fibre and pore structures, whereas the fungal chitin had no pores or fibres. The crystallinity of the insect chitin (CrI=84.9%) was higher than the bryozoan (CrI=60.1%) and fungal chitins (CrI=58.5%).
Department of Biotechnology and Molecular Biology, Aksaray University, 68100 Aksaray, Turkey. Electronic address: muratkaya3806@yahoo.com.
Murat
M
Kaya
Department of Biology, Vytautas Magnus University, LT-44404 Kaunas, Lithuania; Institute of Biology and Plant Biotechnology, Faculty of Agronomy, Aleksandras Stulginskis University, LT-53361 Akademija, Kaunas Region, Lithuania.
Vykintas
V
Baublys
Department of Biology, Vytautas Magnus University, LT-44404 Kaunas, Lithuania.
Ingrida
I
Šatkauskienė
Department of Biotechnology and Molecular Biology, Aksaray University, 68100 Aksaray, Turkey.
Bahar
B
Akyuz
Department of Biotechnology and Molecular Biology, Aksaray University, 68100 Aksaray, Turkey.
Esra
E
Bulut
Department of Biology, Vytautas Magnus University, LT-44404 Kaunas, Lithuania.
Vaida
V
Tubelytė
D000818:Animals; D001181:Arthropods / Q000737:chemistry* / Q000378:metabolism; D002013:Bryozoa / Q000737:chemistry* / Q000378:metabolism; D002686:Chitin / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D055453:Coriolaceae / Q000737:chemistry* / Q000378:metabolism; D003460:Crystallization; D057139:Nanofibers / Q000737:chemistry; D013045:Species Specificity; D017550:Spectroscopy, Fourier Transform Infrared
D002686:Chitin
Bryozoa; Chitin; Extraction; Fungi; Insect; Plumatella