Optimization of carvacrol, rosmarinic, oleanolic and ursolic acid extraction from oregano herbs (Origanum onites L., Origanum vulgare spp. hirtum and Origanum vulgare L.).
The aim of our study was to increase the extraction efficiency of carvacrol, rosmarinic, oleanolic and ursolic acid from the different species of oregano herbs (Origanum onites L., Origanum vulgare spp. hirtum and Origanum vulgare L.). Various extraction methods (ultrasound-assisted, heat-reflux, continuous stirring, maceration, percolation) and extraction conditions (different solvent, material:solvent ratio, extraction temperature, extraction time) were used, and the active substances were determined by HPLC. The lowest content of carvacrol, rosmarinic, oleanolic and ursolic acid was obtained by percolation. During heat-reflux extraction, the content of active substances depended on the solvent used: ethanol/non-aqueous solvent (glycerol or propylene glycol) mixture was more effective compared with ethanol alone. The results showed that for each species of oregano the most optimal extraction method should be selected to maximize the content of biologically active substances in the extracts.
a Department of Drug Technology and Social Pharmacy , Lithuanian University of Health Sciences , Eivenių str. 4, LT-50161, Kaunas , Lithuania.
Justė
J
Baranauskaitė
b Department of Pharmacognosy , Lithuanian University of Health Sciences , Kaunas , Lithuania.
Valdas
V
Jakštas
c Department of Drug Chemistry , Lithuanian University of Health Sciences , Kaunas , Lithuania.
Liudas
L
Ivanauskas
a Department of Drug Technology and Social Pharmacy , Lithuanian University of Health Sciences , Eivenių str. 4, LT-50161, Kaunas , Lithuania.
Dalia M
DM
Kopustinskienė
a Department of Drug Technology and Social Pharmacy , Lithuanian University of Health Sciences , Eivenių str. 4, LT-50161, Kaunas , Lithuania.
Gailutė
G
Drakšienė
d Department of Pharmaceutics, Faculty of Pharmacy , University of Veterinary and Pharmaceutical Sciences , Brno , Czech Republic.
Ruta
R
Masteikova
a Department of Drug Technology and Social Pharmacy , Lithuanian University of Health Sciences , Eivenių str. 4, LT-50161, Kaunas , Lithuania.
Jurga
J
Bernatonienė
D002851:Chromatography, High Pressure Liquid; D002934:Cinnamates / Q000302:isolation & purification*; D000081007:Cymenes; D053630:Depsides / Q000302:isolation & purification*; D015394:Molecular Structure; D039821:Monoterpenes / Q000302:isolation & purification*; D009828:Oleanolic Acid / Q000302:isolation & purification*; D031341:Origanum / Q000737:chemistry* / Q000145:classification; D010936:Plant Extracts / Q000737:chemistry; D012997:Solvents; D014315:Triterpenes / Q000302:isolation & purification*; D000097123:Rosmarinic Acid; D000097245:Ursolic Acid
D002934:Cinnamates; D000081007:Cymenes; D053630:Depsides; D039821:Monoterpenes; D009828:Oleanolic Acid; D010936:Plant Extracts; D012997:Solvents; D014315:Triterpenes; D000097123:Rosmarinic Acid; D000097245:Ursolic Acid; C073316:carvacrol
HPLC; Origanum; carvacrol; extraction; oleanolic acid; rosmarinic acid; ursolic acid