Enzyme-resistant isomalto-oligosaccharides produced from Leuconostoc mesenteroides NRRL B-1426 dextran hydrolysis for functional food application.
The extracellular dextransucrase from Leuconostoc mesenteroides NRRL B-1426 was produced and purified using polyethylene glycol fractionation. In our earlier study, it was reported that L. mesenteroides dextransucrase synthesizes a high-molecular mass dextran (>2 × 10(6)  Da) with ∼85.5% α-(1→6) linear and ∼14.5% α-(1→3) branched linkages. Isomalto-oligosaccharides (IMOs) were synthesized through depolymerization of dextran by the action of dextranase. The degree of polymerization of IMOs was 2-10 as confirmed by mass spectrometry. The nuclear magnetic resonance spectroscopic analysis revealed the presence of α-(1→3) linkages in the synthesized IMOs. The IMOs were resistant to dextranase, α-glucosidase, and α-amylase, and therefore can have potential application as food additives in the functional foods.
Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, India.
Damini
D
Kothari
Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, India.
Arun
A
Goyal
D001709:Biotechnology / Q000379:methods*; D003911:Dextrans / Q000378:metabolism*; D005110:Extracellular Space / Q000201:enzymology; D055951:Functional Food / Q000382:microbiology*; D005964:Glucosyltransferases / Q000378:metabolism*; D006868:Hydrolysis; D000070007:Leuconostoc mesenteroides / Q000166:cytology / Q000378:metabolism*; D009844:Oligosaccharides / Q000096:biosynthesis*; D013696:Temperature
D003911:Dextrans; D009844:Oligosaccharides; D005964:Glucosyltransferases; C020025:dextransucrase
Leuconostoc mesenteroides NRRL B-1426; dextran; dextransucrase; isomalto-oligosaccharides