Glucosamine loaded injectable silk-in-silk integrated system modulate mechanical properties in bovine ex-vivo degenerated intervertebral disc model.
Injectable hydrogels offer a tremendous potential for treatment of degenerated intervertebral disc due to their ability to withstand complex loading, conforming precisely to the defect spaces and eliminating the need for invasive surgical procedures. We have developed an injectable hydrogel platform of N-acetyl-glucosamine (GlcNAc) loaded silk hollow spheres embedded in silk hydrogel for in situ therapeutic release and enhanced mechanical strength. The assembled silk hydrogel provided adequate structural support to the ex vivo degenerated disc model in a cyclic compression test at par with the native tissue. Spatiotemporal release of GlcNAc in a controlled manner from the silk hollow microspheres trigger enhanced proteoglycan production from ADSCs embedded in the composite system. Role of MAPK and SMAD pathways in increasing proteoglycan production have been explored by immunohistological analysis as a result of the action of GlcNAc on the cells, elucidating the potential of injectable silk microsphere-in-silk hydrogel for the regeneration of degenerated disc tissue.
Department of Textile Technology, IIT Delhi, New Delhi, India; Centre for Biomedical Engineering, IIT Delhi, New Delhi, India; Network of Excellence for Functional Biomaterials, National University of Ireland Galway, Ireland.
Sumit
S
Murab
Network of Excellence for Functional Biomaterials, National University of Ireland Galway, Ireland.
Juhi
J
Samal
Network of Excellence for Functional Biomaterials, National University of Ireland Galway, Ireland.
Akshay
A
Shrivastava
Centre for Biomedical Engineering, IIT Delhi, New Delhi, India.
Alok Ranjan
AR
Ray
Network of Excellence for Functional Biomaterials, National University of Ireland Galway, Ireland. Electronic address: abhay.pandit@nuigalway.ie.
Abhay
A
Pandit
Department of Textile Technology, IIT Delhi, New Delhi, India. Electronic address: sghosh08@textile.iitd.ac.in.
Sourabh
S
Ghosh
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D012831:Bombyx; D002417:Cattle; D002470:Cell Survival; D055119:Elastic Modulus; D020869:Gene Expression Profiling; D005944:Glucosamine / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D066298:In Vitro Techniques; D007403:Intervertebral Disc / Q000473:pathology*; D055959:Intervertebral Disc Degeneration / Q000378:metabolism*; D008422:Materials Testing; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D008863:Microspheres; D010316:Particle Size; D011137:Polystyrenes / Q000737:chemistry; D011509:Proteoglycans / Q000737:chemistry; D012038:Regeneration; D012212:Rheology; D047011:Silk / Q000737:chemistry*; D013314:Stress, Mechanical
D001672:Biocompatible Materials; D020100:Hydrogels; D011137:Polystyrenes; D011509:Proteoglycans; D047011:Silk; D005944:Glucosamine
Glucosamine; Injectable hydrogel; Intervertebral disc; Silk hollow microspheres