Cryopreservation of Sambar deer semen in Thailand.
Little is known of the different freezing and thawing techniques for post-thaw survival of spermatozoa in Sambar deer. So, this study determined the effect of seminal plasma, egg yolk and glycerol extenders and their concentrations, plus cooling, freezing, and thawing protocols on the post-thaw quality of their semen. Semen samples were collected by electro-ejaculation from four Thai Sambar deer stags (Cervus unicolor equinus). As evaluated by post-thaw progressive motility and acrosome integrity removal of seminal plasma was beneficial; Tris-egg yolk was the most efficient extender; a 20% egg yolk concentration was better than the 0%, 10%, or 30%; and a 3% glycerol concentration was better than 5%, 7%, or 9%. Using the optimum dilution techniques, semen was loaded in 0.5 ml plastic straws. Cooling times from ambient temperature to 5°C in 3 hr resulted in higher post-thaw progressive motility and acrosome integrity than 1, 2, or 4 hr. Suspending the straws 4 cm above the surface for 15 min before plunging into liquid nitrogen was better than suspending at 2 or 6 cm. For thawing frozen semen, an intermediate thawing (50°C, 8 sec) protocol was more effective than the slower (37°C, 10 sec) or faster (70°C, 5 sec) thawing rates. Timed insemination following estrus synchronization of 10 hinds resulted in six confirmed pregnancies at 60 days. Five hinds delivered live fawns. This study provides an effective approach for semen cryopreservation and artificial insemination (AI), which should be valuable to scientists for genetics and reproductive management of Sambar deer in developing countries.
Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand.
Thevin
T
Vongpralub
Wildlife Conservation Bureau, National Park, Wildlife and Plant Conservation Department, Bangkok, Thailand.
Wittaya
W
Chinchiyanond
Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand.
Pornchai
P
Hongkuntod
Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand.
Pitcharat
P
Sanchaisuriya
Wildlife Conservation Bureau, National Park, Wildlife and Plant Conservation Department, Bangkok, Thailand.
Sanan
S
Liangpaiboon
Wildlife Conservation Bureau, National Park, Wildlife and Plant Conservation Department, Bangkok, Thailand.
Areeya
A
Thongprayoon
Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand.
Noppadon
N
Somphol
D000177:Acrosome / Q000187:drug effects; D000822:Animal Husbandry / Q000379:methods*; D000818:Animals; D015925:Cryopreservation / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000494:pharmacology / Q000592:standards*; D003670:Deer; D007315:Insemination, Artificial / Q000662:veterinary*; D008297:Male; D012662:Semen Preservation / Q000662:veterinary*; D013081:Sperm Motility / Q000187:drug effects; D013785:Thailand
D003451:Cryoprotective Agents
artificial insemination; field condition; frozen semen protocols; tropical deer