Comparative analysis of sphingomyelin synthase 1 gene expression at the transcriptional and translational levels in human tissues.
Sphingomyelin synthase 1 (SMS1) catalyses the biosynthesis of sphingomyelin in eukaryotic cells. We have previously determined the structure of the SGMS1 gene encoding this enzyme and a number of its alternative transcripts. Here, we describe a study of the expression of the full-length SMS1 protein and the sum of the alternative transcripts encoding this protein in human tissues. The SMS1 protein and mRNA levels in tissues differed significantly and were not correlated, implying the active post-transcriptional regulation of SMS1 protein expression. The putative truncated isoforms of the SMS1 protein, which are encoded by a number of alternative transcripts, were not detected by immunoblotting and thus may be absent or present in only small amounts.
Human Molecular Genetics Department, Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 123182, Russia, sudarolg@img.ras.ru.
Olga Yu
OY
Sudarkina
Ivan B
IB
Filippenkov
Ilya B
IB
Brodsky
Svetlana A
SA
Limborska
Lyudmila V
LV
Dergunova
D001665:Binding Sites; D004790:Enzyme Induction; D004794:Enzyme Repression; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D007668:Kidney / Q000201:enzymology; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D009928:Organ Specificity; D014176:Protein Biosynthesis; D034622:RNA Interference; D014158:Transcription, Genetic; D017855:Transferases (Other Substituted Phosphate Groups) / Q000235:genetics* / Q000378:metabolism
D007527:Isoenzymes; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C455440:SGMS1 protein, human; D017855:Transferases (Other Substituted Phosphate Groups)