Evaluating the resolution power of new microsatellites for species identification and stock delimitation in the Cape hakes Merluccius paradoxus and Merluccius capensis (Teleostei: Merlucciidae).
The utility of 15 new and 17 previously published microsatellite markers was evaluated for species identification and stock delimitation in the deep-water hake Merluccius paradoxus and the shallow-water hake Merluccius capensis. A total of 14 microsatellites were polymorphic in M. paradoxus and 10 in M. capensis. Two markers could individually discriminate the species using Bayesian clustering methods and a statistical power analysis showed that the set of markers for each species is likely to detect subtle genetic differentiation (FST < 0·006) that will be valuable to delimit and characterize genetic stocks.
Molecular Ecology and Evolution Programme, Department of Genetics, University of Pretoria, Private bag X20, Hatfield, 0028, South Africa.
T B
TB
Hoareau
A W
AW
Klopper
S M R
SM
Dos Santos
C J
CJ
Oosthuizen
P
P
Bloomer
D000818:Animals; D001499:Bayes Theorem; D016000:Cluster Analysis; D003247:Conservation of Natural Resources; D048388:Gadiformes / Q000145:classification* / Q000235:genetics; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D013045:Species Specificity
Bayesian methods; cross-species amplification; genetic markers; genomic library; power analysis