Trypanosoma cruzi burden, genotypes, and clinical evaluation of Chilean patients with chronic Chagas cardiopathy.
There are currently no biomarkers to assess which patients with chronic indeterminate Chagas disease will develop heart disease and which will spend their entire life in this state. We hypothetize that the parasite burden and Trypanosoma cruzi genotypes are related to the presence of heart disease in patients with Chagas disease. This study is aimed to investigate the parasite burden and T. cruzi genotypes in chagasic cardiopaths versus chagasic individuals without cardiac involvement according to the New York Heart Association. Patients with chronic Chagas disease, 50 with and 50 without cardiopathy (controls), groups A and B, respectively, were submitted to anamnesis, physical examination, and electrocardiogram. Echo-Doppler was performed for group A; all important known causes of cardiopathy were discarded. Xenodiagnosis, conventional PCR, and quantitative PCR were performed on patients of both groups. T. cruzi genotyping was done for 25 patients of group A and 20 of group B. The 50 cardiopaths had 80 electrocardiographic alterations, most of them in grade II of the New York Heart Association classification; 49 were classified in grade I by Echo-Doppler, and only one patient was in grade III. The difference in average parasitemia in patients of groups A and B was not significant. The most frequent T. cruzi DTU found was TcV. The parasite burden and genotype of the groups with and without cardiopathy were similar. Graphical abstract Imagen 1 Chronic chagas cardiopathy chest X-ray heart enlargement Figure 2 Chronic Chagas cardiopathy microaneurism of left ventricle. Cineangiography.
Laboratorio de Parasitología Básico-Clínico, Programa de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Independencia 1027, PO 9183, Santiago 1, Chile, wapt@med.uchile.cl.
Werner
W
Apt
Arturo
A
Arribada
Inés
I
Zulantay
Miguel
M
Saavedra
Eduardo
E
Araya
Aldo
A
Solari
Sylvia
S
Ortiz
Katherine
K
Arriagada
Jorge
J
Rodríguez
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002598:Chagas Cardiomyopathy / Q000453:epidemiology / Q000469:parasitology* / Q000473:pathology; D002677:Chile / Q000453:epidemiology; D002908:Chronic Disease; D004562:Electrocardiography; D005260:Female; D005838:Genotype; D006321:Heart / Q000469:parasitology; D006801:Humans; D008297:Male; D008875:Middle Aged; D018512:Parasitemia; D060888:Real-Time Polymerase Chain Reaction; D014349:Trypanosoma cruzi / Q000235:genetics*