Successful serial imaging of the mouse cerebral arteries using conventional 3-T magnetic resonance imaging.
Serial imaging studies can be useful in characterizing the pathologic and physiologic remodeling of cerebral arteries in various mouse models. We tested the feasibility of using a readily available, conventional 3-T magnetic resonance imaging (MRI) to serially image cerebrovascular remodeling in mice. We utilized a mouse model of intracranial aneurysm as a mouse model of the dynamic, pathologic remodeling of cerebral arteries. Aneurysms were induced by hypertension and a single elastase injection into the cerebrospinal fluid. For the mouse cerebrovascular imaging, we used a conventional 3-T MRI system and a 40-mm saddle coil. We used non-enhanced magnetic resonance angiography (MRA) to detect intracranial aneurysm formation and T2-weighted imaging to detect aneurysmal subarachnoid hemorrhage. A serial MRI was conducted every 2 to 3 days. MRI detection of aneurysm formation and subarachnoid hemorrhage was compared against the postmortem inspection of the brain that was perfused with dye. The imaging times for the MRA and T2-weighted imaging were 3.7±0.5 minutes and 4.8±0.0 minutes, respectively. All aneurysms and subarachnoid hemorrhages were correctly identified by two masked observers on MRI. This MRI-based serial imaging technique was useful in detecting intracranial aneurysm formation and subarachnoid hemorrhage in mice.
Department of Anesthesiology and Intensive Care, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka, Japan.
Hiroshi
H
Makino
Department of Pharmacology, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka, Japan.
Kazuya
K
Hokamura
Faculty of Radiological Technology, Fujita Health University School of Health Sciences, Toyoake, Aichi, Japan.
Takahiro
T
Natsume
Department of Anesthesiology and Intensive Care, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka, Japan.
Tetsuro
T
Kimura
Department of Neurosurgery, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka, Japan.
Yoshinobu
Y
Kamio
Medical Photonics Research Center, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka, Japan.
Yasuhiro
Y
Magata
Department of Neurosurgery, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka, Japan.
Hiroki
H
Namba
Department of Anesthesiology and Intensive Care, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka, Japan.
Takasumi
T
Katoh
Department of Anesthesiology and Intensive Care, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka, Japan.
Shigehito
S
Sato
Department of Anesthesia and Perioperative Care, University of California, San Francisco, San Francisco, California, USA.
Tomoki
T
Hashimoto
Department of Pharmacology, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka, Japan.
Kazuo
K
Umemura
D000818:Animals; D002533:Cerebral Angiography; D002536:Cerebral Arteries / Q000000981:diagnostic imaging* / Q000503:physiopathology; D004195:Disease Models, Animal; D002532:Intracranial Aneurysm / Q000139:chemically induced / Q000000981:diagnostic imaging* / Q000503:physiopathology; D018810:Magnetic Resonance Angiography; D008297:Male; D051379:Mice