Inhibition of glucose-transporter 1 (GLUT-1) expression reversed Warburg effect in gastric cancer cell MKN45.
Glucose transporter-1 (GLUT-1) plays critical roles in cancer development and progression. Warburg effect (aerobic glycolysis) contributes greatly to tumorigenesis and could be targeted for tumor therapy. However, published data on the relationship between GLUT-1 and Warburg effect are scarce. In this study, gastric cancer cell, MKN45, was transfected with GLUT-1 shRNA using Lipofectamine 2000. Oxygen consumption, LDH activity, lactate production and cytoplasmic pyruvate were detected after MKN45 cells with GLUT-1 knockdown. In the last, hexokinase 1 (HK1), HK2, and pyruvate kinase M2 (PKM2) expression were detected by using western blot. In this study, we showed that inhibition of GLUT-1 expression reversed Warburg effect in MKN45 cells, and induced apoptosis.
Department of Biochemistry and Molecular Biology, Preclinical Medicine College, China Medical University Shenyang 110001, Liaoning, P. R. China.
Tian-Biao
TB
Zhang
Department of General Surgery, Shengjing Hospital of China Medical University Shenyang 110004, Liaoning, P. R. China.
Ying
Y
Zhao
Department of Biochemistry and Molecular Biology, Preclinical Medicine College, China Medical University Shenyang 110001, Liaoning, P. R. China.
Zhao-Xue
ZX
Tong
Department of Biochemistry and Molecular Biology, Preclinical Medicine College, China Medical University Shenyang 110001, Liaoning, P. R. China.
Yi-Fu
YF
Guan
GLUT-1; LDH; PKM2; Warburg effect; gastric cancer