Fat and carbohydrate in western diet contribute differently to hepatic lipid accumulation.
We investigated the contributions of dietary fat and dietary carbohydrate to the development of fatty liver induced by western diet (WD). Compared with WD-fed wild type (WT) mice, livers of WD-fed ChREBP(-/-) mice showed lipid droplets of varying sizes around the hepatic lobules, while hepatic triglyceride and cholesterol contents were only modestly decreased. Inflammation and fibrosis were suppressed in ChREBP(-/-) mice. In addition, compared with WD-fed WT mice, ChREBP(-/-) mice showed decreased β-oxidation, ketogenesis and FGF21 production, increased intestinal lipid absorption, and decreased VLDL secretion. These findings suggest that dietary fat and carbohydrate contribute differently to the development of fatty liver.
Department of Diabetes and Endocrinology, Graduate School of Medicine, Gifu University, Gifu 501-1194, Japan.
Wudelehu
W
Wu
Department of Diabetes and Endocrinology, Graduate School of Medicine, Gifu University, Gifu 501-1194, Japan.
Hiromi
H
Tsuchida
Department of Diabetes and Endocrinology, Graduate School of Medicine, Gifu University, Gifu 501-1194, Japan; Matsunami General Hospital, Gifu 501-6062, Japan.
Takehiro
T
Kato
Department of Diabetes and Endocrinology, Graduate School of Medicine, Gifu University, Gifu 501-1194, Japan.
Horoyuki
H
Niwa
Department of Diabetes and Endocrinology, Graduate School of Medicine, Gifu University, Gifu 501-1194, Japan.
Yukio
Y
Horikawa
Department of Diabetes and Endocrinology, Graduate School of Medicine, Gifu University, Gifu 501-1194, Japan.
Jun
J
Takeda
Department of Diabetes and Endocrinology, Graduate School of Medicine, Gifu University, Gifu 501-1194, Japan; Gifu University Hospital Center for Nutritional Support and Infection Control, Gifu 501-1194, Japan. Electronic address: kiizuka@gifu-u.ac.jp.
Katsumi
K
Iizuka
D000818:Animals; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D066273:Diet, Western; D004040:Dietary Carbohydrates / Q000378:metabolism*; D004041:Dietary Fats / Q000378:metabolism*; D005234:Fatty Liver / Q000378:metabolism*; D050356:Lipid Metabolism / Q000502:physiology*; D008099:Liver / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D004040:Dietary Carbohydrates; D004041:Dietary Fats; C506001:Mlxipl protein, mouse; D009687:Nuclear Proteins; D014157:Transcription Factors
ChREBP; FGF21; Hepatic steatosis; Ketogenesis; VLDL; β-oxidation