Promoter DNA methylation is associated with KLF11 expression in epithelial ovarian cancer.
As a transforming growth factor-β (TGF-β)-inducible gene, the expression of Krüppel-like transcription factor 11 (KLF11) is altered in several types of cancer. In the current study, through using human 9K CpG island array, KLF11 was identified as one of hypermethylated genes in RAS-transformed ovarian T29H cells. Methylation of the KLF11 promoter was also observed in ovarian cancer tissue samples accompanied by significantly reduced KLF11 gene expression. Interestingly, the expression of SMAD2, SMAD3, and SMAD7 genes was reduced in the tumour, whilst no change was found in TGF-β expression. Our data suggest a relationship between promoter DNA methylation and KLF11 gene expression in ovarian cancer tumorigenesis.
Department of Obstetrics and Gynecology, Tianjin Central Hospital of Obstetrics and Gynecology, Tianjin, 300100, China.
Guan
G
Wang
State Key Laboratory of Medical Genetics, Central South University, Changsha, Hunan, 410078, China.
Xianfeng
X
Li
Tianjin Research Center of Basic Medical Science, Tianjin Medical University, Tianjin, 300070, China.
Weiping
W
Tian
Institute of Psychology, Chinese Academy of Sciences, Beijing, 100101, China.
Yan
Y
Wang
Department of Obstetrics and Gynecology, Tianjin Central Hospital of Obstetrics and Gynecology, Tianjin, 300100, China.
Dandan
D
Wu
Beijing Institute of Life Science, Chinese Academy of Science, Beijing, 100101, China.
Zhongsheng
Z
Sun
Department of Obstetrics and Gynecology, Chinese PLA General Hospital, Beijing, 100853, China.
Enfeng
E
Zhao
D000368:Aged; D051017:Apoptosis Regulatory Proteins; D000077216:Carcinoma, Ovarian Epithelial; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002681:China; D018899:CpG Islands; D019175:DNA Methylation; D005260:Female; D056726:Genetic Association Studies; D006801:Humans; D008875:Middle Aged; D009375:Neoplasms, Glandular and Epithelial / Q000235:genetics / Q000378:metabolism*; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D051785:Smad Proteins / Q000378:metabolism; D055411:TGF-beta Superfamily Proteins / Q000378:metabolism
D051017:Apoptosis Regulatory Proteins; D018797:Cell Cycle Proteins; C115117:KLF11 protein, human; D012097:Repressor Proteins; D051785:Smad Proteins; D055411:TGF-beta Superfamily Proteins