Characterization of a novel polysaccharide with anti-colon cancer activity from Lactobacillus helveticus MB2-1.
The present study aimed at investigating the potential anti-colon cancer activity of three purified exopolysaccharides fractions (LHEPS-1, LHEPS-2 and LHEPS-3) from the Lactobacillus helveticus MB2-1. The experimental evidence showed that LHEPS-1 significantly inhibited cell proliferation of human colon cancer Caco-2 cells in both time- and concentration-dependent manners. In contrast, no significant improvements of the inhibitory effects of LHEPS-2 and LHEPS-3 on Caco-2 cells were observed with increasing sample concentrations or prolonged incubation time. Furthermore, the structure of LHEPS-1 was elucidated using methylated analysis, gas chromatography-mass spectroscopy (GC-MS) and nuclear magnetic resonance spectroscopy (NMR), including one- and two-dimensional nuclear magnetic resonance (1D and 2D NMR). Results indicated that the LHEPS-1 consisted of a decasaccharide repeating unit with the following structure (n ≈ 122)： Our results suggested that the LHEPS-1 produced by L. helveticus MB2-1 might be suitable for using as natural anti-colon cancer drugs and functional foods ingredients.
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
Wei
W
Li
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
Weizhi
W
Tang
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
Juan
J
Ji
Institute of Agro-Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210095, PR China.
Xiudong
X
Xia
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
Xin
X
Rui
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
Xiaohong
X
Chen
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
Mei
M
Jiang
Institute of Agro-Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210095, PR China.
Jianzhong
J
Zhou
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China. Electronic address: dongms@njau.edu.cn.
Mingsheng
M
Dong
D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology; D018938:Caco-2 Cells; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D003110:Colonic Neoplasms / Q000188:drug therapy; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D052198:Lactobacillus helveticus / Q000737:chemistry*; D008745:Methylation; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000494:pharmacology; D013329:Structure-Activity Relationship
D000970:Antineoplastic Agents; D011135:Polysaccharides, Bacterial
Anti-colon cancer activity; Exopolysaccharides; Lactobacillus helveticus MB2-1; Methylated analysis; Nuclear magnetic resonance spectroscopy; Structural characterization