Physicochemical study of the formation of complexes between pancreatic proteases and polyanions.
The formation of insoluble complexes between proteins and oppositely charged polyelectrolytes was assessed. Two pancreatic enzymes: trypsin and chymotrypsin, and two anionic synthetic polyelectrolytes: polyacrylate and polyvinylsulfonate, were used for the study at the pH range between 3.00 and 5.00. Two different titration curve shapes, representing two insoluble complexes formation mechanisms, were found. The turbidity of enzyme-polyelectrolyte mixtures is related to the increase either in the size or in the quantity of the insoluble complexes. Ionic strength destabilized insoluble complex formation. Finally, the kinetics of the process of insoluble complex formation at different conditions was studied.
Laboratorio de Fisicoquímica Aplicada a Bioseparación, Facultad de Ciencias Bioquímicas y Farmacéuticas, CONICET, Universidad Nacional de Rosario, Suipacha 570, (S2002RLK) Rosario, Argentina.
Julia
J
Lombardi
Laboratorio de Fisicoquímica Aplicada a Bioseparación, Facultad de Ciencias Bioquímicas y Farmacéuticas, CONICET, Universidad Nacional de Rosario, Suipacha 570, (S2002RLK) Rosario, Argentina.
Guillermo
G
Picó
Laboratorio de Fisicoquímica Aplicada a Bioseparación, Facultad de Ciencias Bioquímicas y Farmacéuticas, CONICET, Universidad Nacional de Rosario, Suipacha 570, (S2002RLK) Rosario, Argentina. Electronic address: valeriaboeris@conicet.gov.ar.
Valeria
V
Boeris
D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D011232:Chemical Precipitation; D002918:Chymotrypsin / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009391:Nephelometry and Turbidimetry; D009994:Osmolar Concentration; D010179:Pancreas / Q000737:chemistry / Q000201:enzymology; D011145:Polyvinyls / Q000737:chemistry*; D013451:Sulfonic Acids / Q000737:chemistry*; D014357:Trypsin / Q000737:chemistry*
D000180:Acrylic Resins; D011145:Polyvinyls; D013451:Sulfonic Acids; C008959:lyapolate; C006903:carbopol 940; D002918:Chymotrypsin; D014357:Trypsin
Bovine pancreas serine proteases; Protein–polyelectrolyte interaction