In vitro acaricidal activity of 1,8-cineole against Sarcoptes scabiei var. cuniculi and regulating effects on enzyme activity.
1,8-Cineole found in many essential oils is a monoterpene and acts as a repellent against Sarcoptes scabiei var. cuniculi. In the present study, the acaricidal activity of 1,8-cineole against S. scabiei var. cuniculi was evaluated and the acaricidal mechanism was also investigated by assaying enzyme activities. The results showed that the lethal concentration of 50% (LC50) value (95% confidence limit (CL)) and the lethal time of 50% (LT50) value (95% CL) of 1,8-cineole were 2.77 mg/mL and 3.606 h, respectively. The pathological changes under transmission electron microscopy showed that the morphology of the mitochondria was abnormal, the cell nuclear membrane was damaged, and the nuclear chromatin was dissoluted. The activities of superoxide dismutase (SOD), glutathione-s-transferases (GSTs), monoamine oxidase (MAO), nitric oxide synthase (NOS), and acetylcholinesterase (AChE) were significantly changed after treatment with 1,8-cineole for 4, 8, 12, and 24 h. SOD and GSTs are associated with the protection mechanism of scabies mites. And, the activities of SOD and GSTs were increased as compared with the control group. MAO, AChE, and NOS are associated with the nervous system of scabies mites. The activity of MAO was increased whereas the AChE was suppressed. The activity of NOS was suppressed in the high-dose group whereas increased in the middle-dose group and low-dose group. These results indicated that the mechanism of 1,8-cineole mainly attributed to the changes of these enzyme activities related to the nervous system of scabies mites.
Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, People's Republic of China.
Zhiqiang
Z
Hu
Zhenzhen
Z
Chen
Zhongqiong
Z
Yin
Renyong
R
Jia
Xu
X
Song
Li
L
Li
Yuanfeng
Y
Zou
Xiaoxia
X
Liang
Lixia
L
Li
Changliang
C
He
Lizi
L
Yin
Cheng
C
Lv
Ling
L
Zhao
Gang
G
Su
Gang
G
Ye
Fei
F
Shi
D056810:Acaricides / Q000494:pharmacology*; D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D003511:Cyclohexanols / Q000494:pharmacology*; D000077591:Eucalyptol; D005982:Glutathione Transferase / Q000378:metabolism; D008995:Monoamine Oxidase / Q000378:metabolism; D039821:Monoterpenes / Q000494:pharmacology*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D009822:Oils, Volatile / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology; D012520:Sarcoptes scabiei / Q000187:drug effects* / Q000648:ultrastructure; D013482:Superoxide Dismutase / Q000378:metabolism
D056810:Acaricides; D003511:Cyclohexanols; D039821:Monoterpenes; D009822:Oils, Volatile; D010936:Plant Extracts; D019001:Nitric Oxide Synthase; D013482:Superoxide Dismutase; D008995:Monoamine Oxidase; D005982:Glutathione Transferase; D000110:Acetylcholinesterase; D000077591:Eucalyptol