Comparison of the Efficiencies of Buffers Containing Ankaferd and Chitosan on Hemostasis in an Experimental Rat Model with Femoral Artery Bleeding.
OBJECTIVE
In the first assessment of trauma patients with major vascular injuries, we need effective and rapid-acting homeostatic materials. In this study we compare the efficiencies of Ankaferd Blood Stopper® and a chitosan linear polymer (Celox®) in an experimental rat model with femoral artery bleeding.


METHODS
Thirty male Wistar albino rats weighing 200-250 g were divided into 3 groups: control, Ankaferd, and chitosan. The femoral artery and vein were visualized and bleeding was started by an incision. The bleeding time was recorded and categorized as 'bleeding stopped at the second minute', 'bleeding stopped at the fourth minute', and 'unsuccessful' if bleeding continued after the fourth minute.


RESULTS
In the control group, 60% of the bleeding did not stop. In the first 4 min in the Ankaferd group, the bleeding stopped in all rats; only in 1 of the rats in the chitosan group did the bleeding not stop. In stopping the bleeding in the first 4 min, Ankaferd was similar to chitosan but better than the control group; the chitosan group was similar to the control, but the p-value was close to significance.


CONCLUSIONS
For major arterial bleeding, the main treatment is surgical bleeding control, but outside of the hospital we can use buffers containing Ankaferd and chitosan on the bleeding region. The results of this study should be supported with larger studies. Furthermore, in our study, healthy rats were used. New studies are needed to evaluate the results of hypovolemic and hypotensive cases with major artery bleeding.
Osmaniye State Hospital, Clinic of Emergency, Osmaniye, Turkey. E-mail: serkanabacioglu@gmail.com.
Serkan
S
Abacıoğlu
Kemal
K
Aydın
Fatih
F
Büyükcam
Ural
U
Kaya
Bahattin
B
Işık
Muhammed Evvah
ME
Karakılıç
D000818:Animals; D001704:Biopolymers / Q000627:therapeutic use*; D001780:Blood Coagulation Tests; D002021:Buffers; D004353:Drug Evaluation, Preclinical; D005263:Femoral Artery / Q000293:injuries*; D006470:Hemorrhage / Q000097:blood / Q000188:drug therapy*; D006490:Hemostatics / Q000627:therapeutic use*; D008297:Male; D010936:Plant Extracts / Q000627:therapeutic use*; D011312:Pressure; D051381:Rats; D017208:Rats, Wistar
D001704:Biopolymers; D002021:Buffers; C540163:Celox; D006490:Hemostatics; D010936:Plant Extracts; C539751:ankaferd blood stopper