Generation of large pig and bovine blastocysts by culturing in human induced pluripotent stem cell medium.
We investigated the effect of human induced pluripotent stem cell (hiPS) medium on porcine somatic cell nuclear transfer and bovine in vitro fertilized early blastocysts, in comparison with North Carolina State University (NCSU)-37 medium and in vitro culture (IVC)-II medium. After 2 days of culture, the diameter of the portion of the blastocyst that was extruded from the zona pellucid dramatically differed between porcine blastocysts cultured in hiPS medium and those cultured in NCSU-37 medium (221.47 ± 38.94 μm versus 481.87 ± 40.61 μm, P < 0.01). Moreover, the diameter of the portion of the blastocyst significantly differed between bovine blastocysts cultured in hiPS medium and those cultured in IVC-II medium (150.30 ± 29.49 μm versus 195.58 ± 41.59 μm, P < 0.01). Furthermore, the total number of cells per porcine and bovine blastocyst was more than two-fold higher in blastocysts cultured in hiPS medium than in those cultured in NCSU-37 medium (44.33 ± 5.28 and 143.33 ± 16.05, P < 0.01) or IVC-II medium (172.12 ± 45.08 and 604.83 ± 242.64, P < 0.01), respectively. These results indicate that hiPS medium markedly improves the quality of porcine and bovine blastocysts.
Department of Animal Science,College of Agriculture,Yanbian University,Yanji,133000,China.
Qing-Shan
QS
Gao
Department of Animal Science,College of Agriculture,Yanbian University,Yanji,133000,China.
Long
L
Jin
Department of Animal Science,College of Agriculture,Yanbian University,Yanji,133000,China.
Suo
S
Li
Department of Animal Science,College of Agriculture,Yanbian University,Yanji,133000,China.
Hai-Ying
HY
Zhu
Department of Animal Science,College of Agriculture,Yanbian University,Yanji,133000,China.
Qing
Q
Guo
Department of Animal Science,College of Agriculture,Yanbian University,Yanji,133000,China.
Xiao-Chen
XC
Li
Department of Animal Science,College of Agriculture,Yanbian University,Yanji,133000,China.
Qing-Guo
QG
Jin
Department of Animal Science,College of Agriculture,Yanbian University,Yanji,133000,China.
Jin-Dan
JD
Kang
Department of Animal Science,College of Agriculture,Yanbian University,Yanji,133000,China.
Chang-Guo
CG
Yan
Department of Animal Science,College of Agriculture,Yanbian University,Yanji,133000,China.
Xi-Jun
XJ
Yin
D000818:Animals; D001755:Blastocyst / Q000166:cytology*; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology*; D046149:Embryo Culture Techniques / Q000379:methods / Q000662:veterinary; D047108:Embryonic Development / Q000187:drug effects*; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D008297:Male; D008856:Microscopy, Fluorescence; D009865:Oocytes / Q000166:cytology; D013045:Species Specificity; D013552:Swine; D013997:Time Factors
D003470:Culture Media
Blastocyst cell number; Blastocyst morphological quality; Bovine blastocysts; Porcine blastocysts; hiPS medium