Cytotoxic Lignan from the Non-Transformed Root Culture of Phyllanthus amarus.
A new lignan from the non-transformed root in vitro cultures of Phyllanthus amarus was isolated. The structure of the compound was established on the basis of one- and two-dimensional NMR, as well as mass spectrometry data, as 7'-oxocubebin dimethylether (1,4-bis(benzo[d][1,3]dioxol-5-yl)-2,3-bis(methoxymethyl)butan-1-on). The non-transformed root cultures of P. amarus showed to be a selective source of this compound. The lignan revealed strong cytotoxic activity against HeLa cell line with an IC50 value of 3.8 µg/mL.
Department of Pharmacognosy with Medicinal Plants Garden, Medical University of Gdańsk, Gdańsk 80-210, Poland. bsparzak@gumed.edu.pl.
Barbara
B
Sparzak
Department of Pharmacognosy with Medicinal Plants Garden, Medical University of Gdańsk, Gdańsk 80-210, Poland. krauze@gumed.edu.pl.
Mirosława
M
Krauze-Baranowska
Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Gdańsk 80-822, Poland. anna.kawiak@biotech.ug.edu.pl.
Anna
A
Kawiak
NMR Laboratory, Faculty of Chemistry, Technical University of Gdańsk, Gdańsk 80-233, Poland. pawel.sowinski@pg.gda.pl.
Paweł
P
Sowiński
D045744:Cell Line, Tumor; D006367:HeLa Cells; D006801:Humans; D017705:Lignans / Q000737:chemistry* / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D029803:Phyllanthus / Q000737:chemistry*; D008517:Phytotherapy / Q000379:methods; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry*
D017705:Lignans; D010936:Plant Extracts