Fluorescent peptide-based sensors for the ratiometric detection of nanomolar concentration of heparin in aqueous solutions and in serum.
New fluorescent peptide-based sensors (1-3) for monitoring heparin in serum sample were synthesized using short peptides (1∼3mer) as a receptor. The peptide-based sensors (2 and 3) showed a sensitive ratiometric response to heparin both in aqueous buffered solution (10 mM HEPES, pH 7.4) and in 2% human serum sample by increase of excimer emission of pyrene at 480 nm and concomitant decrease of monomer emission of pyrene at 376 nm, whereas the peptide-based sensor 1 showed a turn off response only by decrease of monomer emission at 376 nm. 2 and 3 exhibited excellent selectivity toward heparin among various anions and competitors of heparin including chondroitin 4-sulfate (ChS) and hyaluronic acid (HA). Peptide-based sensor 3 showed a more sensitive response to heparin than 2. The detection limit of 3 was determined as 36 pM (R(2) = 0.998) for heparin in aqueous solution and 204 pM (R(2) = 0.999) for heparin in aqueous solutions containing 2% human serum. The peptide-based sensors, 2 and 3 provided a practical and potential tool for the detection and quantification of heparin in real biological samples.
Bioorganic Chemistry Laboratory, Center for Design and Applications of Molecular Catalysts, Department of Chemistry and Chemical Engineering, Inha University, 253 Yonghyun-Dong, Nam-Gu, Incheon, 402-751, Korea.
Ponnaboina
P
Thirupathi
Bioorganic Chemistry Laboratory, Center for Design and Applications of Molecular Catalysts, Department of Chemistry and Chemical Engineering, Inha University, 253 Yonghyun-Dong, Nam-Gu, Incheon, 402-751, Korea.
Lok Nath
LN
Neupane
Bioorganic Chemistry Laboratory, Center for Design and Applications of Molecular Catalysts, Department of Chemistry and Chemical Engineering, Inha University, 253 Yonghyun-Dong, Nam-Gu, Incheon, 402-751, Korea. Electronic address: leekh@inha.ac.kr.
Keun-Hyeung
KH
Lee
D000925:Anticoagulants / Q000032:analysis / Q000097:blood*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006493:Heparin / Q000032:analysis / Q000097:blood*; D006801:Humans; D057230:Limit of Detection; D010455:Peptides / Q000737:chemistry*; D011721:Pyrenes / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods
D000925:Anticoagulants; D005456:Fluorescent Dyes; D010455:Peptides; D011721:Pyrenes; D006493:Heparin; C030984:pyrene
Biosensor; Chemosensor; Fluorescence; Heparin; Peptide; Ratiometric