High-throughput screening assays for antibacterial and antifungal activities of Lactobacillus species.
We describe high-throughput screening techniques to rapidly detect either antimicrobial activity, using an agar-well diffusion assay in microtiter plates, or antifungal activity using an agar-spot assay in 24-well plates. 504 Lactobacillus isolates were screened with minimal laboratory equipment and screening rates of 2000-5000 individual antimicrobial interactions.
Laboratory of Food Biotechnology, Institute of Food, Nutrition and Health, ETH Zurich, Schmelzbergstrasse 7, 8092 Zurich, Switzerland.
Raffael C
RC
Inglin
Laboratory of Food Biotechnology, Institute of Food, Nutrition and Health, ETH Zurich, Schmelzbergstrasse 7, 8092 Zurich, Switzerland.
Marc J A
MJ
Stevens
Laboratory of Food Biotechnology, Institute of Food, Nutrition and Health, ETH Zurich, Schmelzbergstrasse 7, 8092 Zurich, Switzerland.
Lukas
L
Meile
Laboratory of Food Biotechnology, Institute of Food, Nutrition and Health, ETH Zurich, Schmelzbergstrasse 7, 8092 Zurich, Switzerland.
Christophe
C
Lacroix
Laboratory of Food Biotechnology, Institute of Food, Nutrition and Health, ETH Zurich, Schmelzbergstrasse 7, 8092 Zurich, Switzerland. Electronic address: leo.meile@hest.ethz.ch.
Leo
L
Meile
D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology; D000898:Antibiosis; D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology; D057166:High-Throughput Screening Assays / Q000379:methods*; D007778:Lactobacillus / Q000378:metabolism / Q000502:physiology*
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents
Antibacterial; Antifungal; Bacteriocin; High-throughput screening; Lactobacillus