Proteomics of human aqueous humor.
The aqueous humor is a colorless, transparent fluid that fills the anterior chamber of the eye. It plays an important role in maintaining the intraocular pressure and providing nourishment to the lens and cornea. The constitution of the aqueous humor is controlled by the blood-aqueous barrier. Though this ocular fluid has been extensively studied, its role in ocular physiology is still not completely understood. In this study, aqueous humor samples were collected from 250 patients undergoing cataract surgery, subjected to multiple fractionation strategies and analyzed on a Fourier transform LTQ-Orbitrap Velos mass spectrometer. In all, we identified 763 proteins, of which 386 have been identified for the first time in this study. Sorbitol dehydrogenase (SORD), filensin (BFSP1), and phakinin (BFSP2) are some of the proteins that have not been previously reported in the aqueous humor. Gene Ontology analysis revealed 35% of the identified proteins to be extracellular, with a majority of them involved in cell communication and signal transduction. This study comprehensively reports 386 novel proteins that have important potential as biomarker candidates for future research into personalized medicine and diagnostics aimed towards improving visual health.
1 Institute of Bioinformatics , International Tech Park, Bangalore, India .
Krishna R
KR
Murthy
Pavithra
P
Rajagopalan
Sneha M
SM
Pinto
Jayshree
J
Advani
Praveen R
PR
Murthy
Renu
R
Goel
Yashwanth
Y
Subbannayya
Lavanya
L
Balakrishnan
Mahashweta
M
Dash
Abhijith K
AK
Anil
Srikanth S
SS
Manda
Raja Sekhar
RS
Nirujogi
Dhanashree S
DS
Kelkar
Gajanan J
GJ
Sathe
Gourav
G
Dey
Aditi
A
Chatterjee
Harsha
H
Gowda
Shukti
S
Chakravarti
Subramanian
S
Shankar
Nandini A
NA
Sahasrabuddhe
Bipin
B
Nair
Babu Lal
BL
Somani
T S Keshava
TS
Prasad
Akhilesh
A
Pandey
D001082:Aqueous Humor / Q000737:chemistry*; D002853:Chromatography, Liquid; D005136:Eye Proteins / Q000032:analysis; D006801:Humans; D007381:Intermediate Filament Proteins / Q000032:analysis; D007064:L-Iditol 2-Dehydrogenase / Q000032:analysis; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry
D005136:Eye Proteins; D007381:Intermediate Filament Proteins; C079098:filensin; C084808:phakinin; D007064:L-Iditol 2-Dehydrogenase