An in situ Raman spectroscopy-based microfluidic "lab-on-a-chip" platform for non-destructive and continuous characterization of Pseudomonas aeruginosa biofilms.
Pseudomonas aeruginosa biofilm was cultivated and characterized in a microfluidic "lab-on-a-chip" platform coupled with confocal Raman microscopy in a non-destructive manner. Biofilm formation could be quantified by this label-free platform and correlated well with confocal laser scanning microscopy. This Raman-microfluidic platform could also discriminate biofilms at different developmental stages.
Food, Nutrition, and Health Program, Faculty of Land and Food Systems, The University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada. xiaonan.lu@ubc.ca.
Jinsong
J
Feng
César
C
de la Fuente-Núñez
Michael J
MJ
Trimble
Jie
J
Xu
Robert E W
RE
Hancock
Xiaonan
X
Lu
D018441:Biofilms; D056656:Lab-On-A-Chip Devices; D046210:Microfluidic Analytical Techniques; D018613:Microscopy, Confocal; D025341:Principal Component Analysis; D011550:Pseudomonas aeruginosa / Q000737:chemistry* / Q000502:physiology; D013059:Spectrum Analysis, Raman