Real-time monitoring of the mitophagy process by a photostable fluorescent mitochondrion-specific bioprobe with AIE characteristics.
An isothiocyanate-functionalized tetraphenylethene is synthesized and used as a fluorescent bioprobe for mitochondrion imaging with high specificity and photostability. The covalent conjugation of the bioprobe to mitochondrial proteins endows it with high resistance to microenvironmental changes, enabling it for real-time monitoring of mitophagy.
Department of Hepatobiliary Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. qc_zheng@mail.hust.edu.cn.
Weijie
W
Zhang
Ryan T K
RT
Kwok
Yilong
Y
Chen
Sijie
S
Chen
Engui
E
Zhao
Chris Y Y
CY
Yu
Jacky W Y
JW
Lam
Qichang
Q
Zheng
Ben Zhong
BZ
Tang
D000114:Acetylene / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D015374:Biosensing Techniques; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D017879:Isothiocyanates / Q000737:chemistry; D018613:Microscopy, Confocal; D008928:Mitochondria / Q000378:metabolism*; D063306:Mitophagy; D014466:Ultraviolet Rays
D005456:Fluorescent Dyes; D017879:Isothiocyanates; C044736:phenylacetylene; C037152:isothiocyanic acid; D000114:Acetylene