Biocompatible patterning of proteins on wettability gradient surface by thermo-transfer printing.
We develop a simple and biocompatible method of patterning proteins on a wettability gradient surface by thermo-transfer printing. The wettability gradient is produced on a poly(dimethylsiloxane) (PDMS)-modified glass substrate through the temperature gradient during thermo-transfer printing. The water contact angle on the PDMS-modified surface is found to gradually increase along the direction of the temperature gradient from a low to a high temperature region. Based on the wettability gradient, the gradual change in the adsorption and immobilization of proteins (cholera toxin B subunit) is achieved in a microfluidic cell with the PDMS-modified surface.
Sungho
S
Kim
Yong-Sang
YS
Ryu
Jeng-Hun
JH
Suh
Chang-Min
CM
Keum
Youngjoo
Y
Sohn
Sin-Doo
SD
Lee
D001672:Biocompatible Materials; D002772:Cholera Toxin / Q000737:chemistry*; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D044085:Microfluidics; D013499:Surface Properties; D017155:Wettability
D001672:Biocompatible Materials; D004129:Dimethylpolysiloxanes; C013830:baysilon; D002772:Cholera Toxin