An automated imaging system for radiation biodosimetry.
We describe here an automated imaging system developed at the Center for High Throughput Minimally Invasive Radiation Biodosimetry. The imaging system is built around a fast, sensitive sCMOS camera and rapid switchable LED light source. It features complete automation of all the steps of the imaging process and contains built-in feedback loops to ensure proper operation. The imaging system is intended as a back end to the RABiT-a robotic platform for radiation biodosimetry. It is intended to automate image acquisition and analysis for four biodosimetry assays for which we have developed automated protocols: The Cytokinesis Blocked Micronucleus assay, the γ-H2AX assay, the Dicentric assay (using PNA or FISH probes) and the RABiT-BAND assay.
Department of Radiation Oncology, Radiological Research Accelerator Facility, Columbia University, Irvington, New York, 10533.
Guy
G
Garty
Department of Radiation Oncology, Radiological Research Accelerator Facility, Columbia University, Irvington, New York, 10533.
Alan W
AW
Bigelow
Department of Radiation Oncology, Center for Radiological Research, Columbia University, New York, New York, 10032.
Mikhail
M
Repin
Department of Radiation Oncology, Center for Radiological Research, Columbia University, New York, New York, 10032.
Helen C
HC
Turner
Department of Mechanical Engineering, Columbia University, New York, New York, 10027.
Dakai
D
Bian
Department of Radiation Oncology, Center for Radiological Research, Columbia University, New York, New York, 10032.
Adayabalam S
AS
Balajee
Department of Radiation Oncology, Radiological Research Accelerator Facility, Columbia University, Irvington, New York, 10533.
Oleksandra V
OV
Lyulko
Department of Radiation Oncology, Center for Radiological Research, Columbia University, New York, New York, 10032.
Maria
M
Taveras
Department of Mechanical Engineering, Columbia University, New York, New York, 10027.
Y Lawrence
YL
Yao
Department of Radiation Oncology, Center for Radiological Research, Columbia University, New York, New York, 10032.
David J
DJ
Brenner
D001331:Automation / Q000379:methods*; D048749:Cytokinesis; D006657:Histones / Q000378:metabolism; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation / Q000379:methods*; D015162:Micronucleus Tests; D048631:Micronucleus, Germline / Q000737:chemistry* / Q000528:radiation effects; D011874:Radiometry
D006657:Histones
dicentrics; fluorescence microscopy; mBAND; micronuclei; sCMOS; γ-H2AX