Effect of Culture Conditions on Metabolite Production of Xylaria sp.
Seeking a strategy for triggering the cryptic natural product biosynthesis to yield novel compounds in the plant-associated fungus Xylaria sp., the effect of culture conditions on metabolite production was investigated. A shift in the production of five known cytochalasin-type analogues 1-5 to six new α-pyrone derivatives, xylapyrones A-F (compounds 6-11), from a solid to a liquid medium was observed. These compounds were identified by analysis of 1D and 2D NMR and HRMS data. Compounds 1-3 showed moderate cytotoxicity against HepG2 and Caski cancer cell lines with IC50 values ranging from 25 to 63 μM and compounds 4-11 were found to be inactive, with IC50 values>100 μM.
Hubei Key Laboratory of Natural Products Research and Development, College of Biological and Pharmaceutical Sciences, China Three Gorges University, Yichang 443002, China. zhq6396549@163.com.
Hongqi
H
Zhang
Hubei Key Laboratory of Natural Products Research and Development, College of Biological and Pharmaceutical Sciences, China Three Gorges University, Yichang 443002, China. d.zhangshuang@gmail.com.
Zhangshuang
Z
Deng
Hubei Key Laboratory of Natural Products Research and Development, College of Biological and Pharmaceutical Sciences, China Three Gorges University, Yichang 443002, China. zhiyongguoctgu@hotmail.com.
Zhiyong
Z
Guo
The First College of Clinical Medical Science, China Three Gorges University, Yichang 443002, China. ycpy0303@126.com.
Yan
Y
Peng
Hubei Key Laboratory of Natural Products Research and Development, College of Biological and Pharmaceutical Sciences, China Three Gorges University, Yichang 443002, China. hny115@126.com.
Nianyu
N
Huang
Hubei Key Laboratory of Natural Products Research and Development, College of Biological and Pharmaceutical Sciences, China Three Gorges University, Yichang 443002, China. haibohe2001@gmail.com.
Haibo
H
He
Hubei Key Laboratory of Natural Products Research and Development, College of Biological and Pharmaceutical Sciences, China Three Gorges University, Yichang 443002, China. tuxuan7@126.com.
Xuan
X
Tu
Hubei Key Laboratory of Natural Products Research and Development, College of Biological and Pharmaceutical Sciences, China Three Gorges University, Yichang 443002, China. kzou@ctgu.edu.cn.
Kun
K
Zou
D001688:Biological Products / Q000737:chemistry* / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods; D045744:Cell Line, Tumor; D003572:Cytochalasins / Q000737:chemistry* / Q000378:metabolism*; D056945:Hep G2 Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013098:Xylariales / Q000737:chemistry* / Q000378:metabolism*
D001688:Biological Products; D003572:Cytochalasins