Choline Essentiality and Its Requirement in Diets for Juvenile Parrot Fish (Oplegnathus fasciatus).
A 12-wk feeding trial was conducted to evaluate the essentiality of choline supplementation in diets for parrot fish. Five isonitrogenous and isocaloric diets were supplemented with 0 (as control), 500, 1,000, and 2,000 mg choline per kg diet, and a positive control diet without choline contained 0.3% of 2-amino-2-methyl-1-propanol as choline biosynthesis inhibitor (designated as Con, C500, C1000, C2000 and Con(+), respectively). Triplicate groups of fish (body weight, 8.8±0.01 g) were fed one of the experimental diets at a rate of 4% body weight twice daily. The fish fed Con(+) diet revealed significantly lower growth performance and feed utilization efficiency than other fish groups. Supplementation of choline to the basal diet did not significantly influence fish growth. The highest liver lipid content was observed in fish fed the Con(+) diet and inversely correlated with liver choline concentration although the differences were not significant. Also, significantly higher liver linoleic, eicosapentaenoic and docosahexaenoic acid contents were found in fish fed the Con(+) diet. Innate immune parameters including respiratory burst and myeloperoxidase activities were not significantly affected by dietary choline levels. The findings in this study conclude that choline concentration of approximately 230 mg kg(-1) diet meets the requirement of parrot fish.
Department of Marine Life Science, Jeju National University, Jeju 690-756, Korea .
Sanaz
S
Khosravi
Department of Marine Life Science, Jeju National University, Jeju 690-756, Korea .
Ji-Woong
JW
Jang
Department of Marine Life Science, Jeju National University, Jeju 690-756, Korea .
Samad
S
Rahimnejad
Department of Marine Life Science, Jeju National University, Jeju 690-756, Korea .
Jin-Woo
JW
Song
Department of Marine Life Science, Jeju National University, Jeju 690-756, Korea .
Kyeong-Jun
KJ
Lee
Choline; Growth Performance; Oplegnathus fasciatus; Parrot Fish; Requirement