Use of Pollen Solid-Phase Extraction for the Determination of trans-Resveratrol in Peanut Oils.
In this study, a simple and convenient method for the determination of trans-resveratrol (TRA) in peanut oils based on pollen grain solid-phase extraction (SPE) was developed. Pollen grains were used as normal-phase SPE sorbent to separate TRA from peanut oils for the first time. As a naturally occurring material, pollen grains exhibited an excellent adsorption capacity for polyphenolic compounds due to their particular functional structures such as hydroxyl groups, saturated and unsaturated aliphatic chains with aromatics. Their stable compositions as well as adequate particle size (30-40 μm) also make them suitable for SPE. Several parameters influencing extraction performance were investigated. Coupled with high-performance liquid chromatography-ultraviolet detection (HPLC-UV), a green purification method for fast determination of TRA in peanut oils using pollen grain cartridges as sorbents was established. The linearity range of the proposed method was 10-2500 ng · g(-1) with a satisfactory correlation coefficient (r(2)) of 0.9999. The limit of detection (LOD) for TRA in peanut oils was 2.7 ng · g(-1), and the recoveries in spiked oil samples were from 70.2% to 98.4% with the relative standard deviations (RSDs) less than 4.9% (intraday) and 5.2% (interday). This method was successfully applied to the analysis of TRA in several peanut oils with different brands from local market as well as other kinds of vegetable oils.
†Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, China.
Qian
Q
Lu
‡Department of Preventive Medicine, School of Medicine, Wuhan University of Science and Technology, Wuhan 430074, China.
Qin
Q
Zhao
†Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, China.
Qiong-Wei
QW
Yu
†Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, China.
Yu-Qi
YQ
Feng
D000327:Adsorption; D007536:Isomerism; D057230:Limit of Detection; D000074241:Peanut Oil; D028223:Pinus; D010938:Plant Oils / Q000737:chemistry*; D011058:Pollen / Q000737:chemistry*; D000077185:Resveratrol; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*; D013267:Stilbenes / Q000737:chemistry / Q000302:isolation & purification*
D000074241:Peanut Oil; D010938:Plant Oils; D013267:Stilbenes; D000077185:Resveratrol
HPLC-UV; normal-phase solid-phase extraction; peanut oils; pollen grains; trans-resveratrol