WEHI-3 cells inhibit adipocyte differentiation in 3T3-L1 cells.
By investigating the anti-adipogenic effects of WEHI-3 cells - a murine acute myelomonocytic leukemia cell line - we sought to improve the efficiency of hematopoietic stem cell transplantation (HSCT). Analysis of Oil Red O staining and the expression of adipogenic genes, including PPARγ, C/EBPα, FAS and LPL, indicated that WEHI-3 cells significantly inhibited 3T3-L1 mouse preadipocyte cells from differentiating into adipocytes. In vivo, fat vacuoles in mice injected with WEHI-3 cells were also remarkably reduced in the murine bone marrow pimelosis model. Moreover, the key gene in the Rho signaling pathway, ROCKII, and the key gene in the Wnt signaling pathway, β-catenin, were both upregulated compared with the control group. siRNA-mediated knockdown of ROCKII and β-catenin reversed these WEHI-3-mediated anti-adipogenic effects. Taken together, these data suggest that WEHI-3 cells exert anti-adipogenic effects and that both ROCKII and β-catenin are involved in this process.
The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, China.
Jing
J
Lai
Institute of Hematology, School of Medicine, Jinan University, Guangzhou, Guangdong, China.
Gexiu
G
Liu
The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, China.
Guoyao
G
Yan
Institute of Hematology, School of Medicine, Jinan University, Guangzhou, Guangdong, China.
Dongmei
D
He
The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, China.
Ying
Y
Zhou
The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, China. Electronic address: shengtingchen@sina.cn.
Shengting
S
Chen
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism*; D050156:Adipogenesis / Q000235:genetics / Q000502:physiology; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D002460:Cell Line; D018920:Coculture Techniques; D005260:Female; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D018380:Hematopoietic Stem Cell Transplantation; D051379:Mice; D008807:Mice, Inbred BALB C; D008954:Models, Biological; D047495:PPAR gamma / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D015398:Signal Transduction; D060449:Wnt Signaling Pathway; D051176:beta Catenin / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D054460:rho-Associated Kinases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism
D022763:CCAAT-Enhancer-Binding Protein-alpha; C495267:CTNNB1 protein, mouse; D047495:PPAR gamma; D034741:RNA, Small Interfering; D051176:beta Catenin; C515855:Rock2 protein, mouse; D054460:rho-Associated Kinases
3T3-L1; Adipogenesis; Inhibit; WEHI-3