Rapid genotyping with DNA micro-arrays for high-density linkage mapping and QTL mapping in common buckwheat (Fagopyrum esculentum Moench).
For genetic studies and genomics-assisted breeding, particularly of minor crops, a genotyping system that does not require a priori genomic information is preferable. Here, we demonstrated the potential of a novel array-based genotyping system for the rapid construction of high-density linkage map and quantitative trait loci (QTL) mapping. By using the system, we successfully constructed an accurate, high-density linkage map for common buckwheat (Fagopyrum esculentum Moench); the map was composed of 756 loci and included 8,884 markers. The number of linkage groups converged to eight, which is the basic number of chromosomes in common buckwheat. The sizes of the linkage groups of the P1 and P2 maps were 773.8 and 800.4 cM, respectively. The average interval between adjacent loci was 2.13 cM. The linkage map constructed here will be useful for the analysis of other common buckwheat populations. We also performed QTL mapping for main stem length and detected four QTL. It took 37 days to process 178 samples from DNA extraction to genotyping, indicating the system enables genotyping of genome-wide markers for a few hundred buckwheat plants before the plants mature. The novel system will be useful for genomics-assisted breeding in minor crops without a priori genomic information.
Graduate School of Agricultural and Life Sciences, The University of Tokyo , 1-1-1 Yayoi, Bunkyo, Tokyo 113-8657 , Japan.
Shiori
S
Yabe
Graduate School of Life and Environmental Sciences, University of Tsukuba , 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572 , Japan.
Takashi
T
Hara
Graduate School of Agriculture, Kyoto University , Kitashirakawa, Kyoto 606-8502 , Japan.
Mariko
M
Ueno
Future Project Division, TOYOTA MOTOR CORPORATION , 1 Toyota-cho, Toyota, Aichi 471-8572 , Japan.
Hiroyuki
H
Enoki
Future Project Division, TOYOTA MOTOR CORPORATION , 1 Toyota-cho, Toyota, Aichi 471-8572 , Japan.
Tatsuro
T
Kimura
Future Project Division, TOYOTA MOTOR CORPORATION , 1 Toyota-cho, Toyota, Aichi 471-8572 , Japan.
Satoru
S
Nishimura
Graduate School of Agriculture, Kyoto University , Kitashirakawa, Kyoto 606-8502 , Japan.
Yasuo
Y
Yasui
Graduate School of Life and Environmental Sciences, University of Tsukuba , 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572 , Japan.
Ryo
R
Ohsawa
Graduate School of Agricultural and Life Sciences, The University of Tokyo , 1-1-1 Yayoi, Bunkyo, Tokyo 113-8657 , Japan.
Hiroyoshi
H
Iwata
Fagopyrum esculentum Moench; QTL analysis; array-based genotyping system; common buckwheat; genome-wide linkage analysis