[Lipid and fatty acid profiles of Pseudomonas aurantiaca DNA-bound lipids determined by mass spectrometry].
An approach used on investigation of the lipid composition of loosely (fraction 1) and tightly (fraction 2) DNA-bound lipids of Pseudomonas aurantiaca cells by electrospray ionization using mass spectrometry (ESI-LC-MS) was used for determination of the lipidom of a prokaryotic cell. Free fatty acids C16:0, C18:1 (fraction 1), C14:0, C16:0, and C18:2 (fraction 2) were detected. Both fractions of DNA-bound lipids were characterized by the presence of phosphatidylglycerol, phosphatidylserine, and lyso-phosphatidylinositol. The alcohol-soluble fraction 1 could also contain phosphatidylcholine and phosphatidylinositol, while fraction 2 probably contained triacylglycerides. Compared to gas chromatography, ESI-LC-MS provides new possibilities for investigation of the nucleoid lipidome, providing for more detailed investigation of DNA-bound lipids in bacterial cells.
R I
RI
Zhdanov
D
D
Kern
V
V
Lorents
M Ia
MIa
Ibragimova
D002458:Cell Fractionation; D004269:DNA, Bacterial / Q000737:chemistry*; D005230:Fatty Acids, Nonesterified / Q000737:chemistry*; D008246:Lysophospholipids / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry; D010715:Phosphatidylglycerols / Q000737:chemistry; D010716:Phosphatidylinositols / Q000737:chemistry; D010718:Phosphatidylserines / Q000737:chemistry; D011549:Pseudomonas / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
D004269:DNA, Bacterial; D005230:Fatty Acids, Nonesterified; D008246:Lysophospholipids; D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; D010716:Phosphatidylinositols; D010718:Phosphatidylserines; C025449:lysophosphatidylinositol