Deletion of Osr2 Partially Rescues Tooth Development in Runx2 Mutant Mice.
Tooth organogenesis depends on genetically programmed sequential and reciprocal inductive interactions between the dental epithelium and neural crest-derived mesenchyme. Previous studies showed that the Msx1 and Runx2 transcription factors are required for activation of odontogenic signals, including Bmp4 and Fgf3, in the early tooth mesenchyme to drive tooth morphogenesis through the bud-to-cap transition and that Runx2 acts downstream of Msx1 to activate Fgf3 expression. Recent studies identified Osr2 as a repressor of tooth development and showed that inactivation of Osr2 rescued molar tooth morphogenesis in the Msx1(-/-) mutant mice as well as in mice with neural crest-specific inactivation of Bmp4. Here we show that Runx2 expression is expanded in the tooth bud mesenchyme in Osr2(-/-) mutant mouse embryos and is partially restored in the tooth mesenchyme in Msx1(-/-)Osr2(-/-) mutants in comparison with Msx1(-/-) and wild-type embryos. Whereas mandibular molar development arrested at the bud stage and maxillary molar development arrested at the bud-to-cap transition in Runx2(-/-) mutant mice, both mandibular and maxillary molar tooth germs progressed to the early bell stage, with rescued expression of Msx1 and Bmp4 in the dental papilla as well as expression of Bmp4, p21, and Shh in the primary enamel knot in the Osr2(-/-)Runx2(-/-) compound mutants. In contrast to the Msx1(-/-)Osr2(-/-) compound mutants, which exhibit nearly normal first molar morphogenesis, the Osr2(-/-)Runx2(-/-) compound mutant embryos failed to activate the expression of Fgf3 and Fgf10 in the dental papilla and exhibited significant deficit in cell proliferation in both the dental epithelium and mesenchyme in comparison with the control embryos. These data indicate that Runx2 synergizes with Msx1 to drive tooth morphogenesis through the bud-to-cap transition and that Runx2 controls continued tooth growth and morphogenesis beyond the cap stage through activation of Fgf3 and Fgf10 expression in the dental papilla.
Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.
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Kwon
Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA Department of Oral Pathology, School of Dentistry, Kyungpook National University, Daegu, Republic of Korea.
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Park
Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA School of Dentistry, University of Utah, Salt Lake City, UT, USA.
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Jia
Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.
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Liu
Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA Division of Plastic Surgery, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.
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Lan
Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA Division of Plastic Surgery, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA Rulang.Jiang@CCHMC.org.
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Jiang
D000818:Animals; D055415:Bone Morphogenetic Protein 4 / Q000378:metabolism; D016923:Cell Death; D049109:Cell Proliferation; D050659:Core Binding Factor Alpha 1 Subunit / Q000172:deficiency / Q000378:metabolism*; D005260:Female; D051526:Fibroblast Growth Factor 10 / Q000378:metabolism; D051518:Fibroblast Growth Factor 3 / Q000378:metabolism; D020287:In Situ Nick-End Labeling; D051957:MSX1 Transcription Factor / Q000378:metabolism; D051379:Mice; D009805:Odontogenesis / Q000502:physiology*; D011247:Pregnancy; D014070:Tooth / Q000196:embryology* / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*
C525810:Bmp4 protein, mouse; D055415:Bone Morphogenetic Protein 4; D050659:Core Binding Factor Alpha 1 Subunit; C496464:Fgf10 protein, mouse; C489197:Fgf3 protein, mouse; D051526:Fibroblast Growth Factor 10; D051518:Fibroblast Growth Factor 3; D051957:MSX1 Transcription Factor; C484084:Msx1 protein, mouse; C438618:Osr2 protein, mouse; C493716:Runx2 protein, mouse; D014157:Transcription Factors
Bmp4; Fgf10; Fgf3; Msx1; genetic interaction; odontogenesis