The plant cell inhibitor KRP6 is involved in multinucleation and cytokinesis disruption in giant-feeding cells induced by root-knot nematodes.
The plant cell cycle inhibitor gene KRP6 has been investigated in roots infected by plant-parasitic root-knot nematodes (Meloidogyne spp.). Unexpectedly, KRP6 overexpressing lines revealed a distinct role for this specific KRP as an activator of the mitotic cell cycle. This function was confirmed in Arabidopsis thaliana suspension cultures ectopically expressing KRP6. A blockage in the mitotic exit was observed in cell suspensions and in giant cells resulted in the appearance of multi-nucleated cells. KRP6 expression during nematode infection and the similarity in phenotypes among KRP6 overexpressing cell cultures and giant-cell morphology strongly suggest that KRP6 is involved in multinucleation and acytokinesis occurring in giant-cells. Once again nematodes have been shown to manipulate the plant cell cycle machinery in order to promote gall establishment.
a Lab. Nematologia/ICAAM-Instituto de Ciências Agrárias e Ambientais Mediterrânicas ; Universidade de Évora ; Núcleo da Mitra ; Évora , Portugal.
Paulo
P
Vieira
Janice
J
de Almeida Engler
D000818:Animals; D017360:Arabidopsis / Q000166:cytology* / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D048429:Cell Size; D048749:Cytokinesis; D062951:Endoreduplication; D017343:Genes, Plant; D060434:Herbivory; D008938:Mitosis; D059828:Plant Cells / Q000378:metabolism*; D018517:Plant Roots / Q000469:parasitology*; D010941:Plant Tumors; D011003:Ploidies; D012098:Reproduction; D014431:Tylenchoidea / Q000502:physiology*
D029681:Arabidopsis Proteins; D002352:Carrier Proteins; C587773:KRP6 protein, Arabidopsis
arabidopsis thaliana; cyclin dependent kinase; giant cells; kip-related proteins; meloidogyne incognita; mitosis