Inhibition of the aggregation of lactoferrin and (-)-epigallocatechin gallate in the presence of polyphenols, oligosaccharides, and collagen peptide.
The aggregation of lactoferrin and (-)-epigallocatechin gallate (EGCG) was inhibited by polyphenols, oligosaccharides, and collagen peptide in this study. Polyphenols, oligosaccharides, or collagen peptide can effectively prevent the formation of lactoferrin-EGCG aggregates, respectively. The addition sequence of lactoferrin, polyphenols (oligosaccharides or collagen peptide) and EGCG can affect the turbidity and particle size of the ternary complexes in the buffer solution; however, it hardly affected the ζ-potential and fluorescence characteristics. With either positive or negative charge, polyphenols and collagen peptide disrupted the formation of lactoferrin-EGCG aggregate mainly through the mechanism of its competition with EGCG molecules which surrounded the lactoferrin molecule surface with weaker binding affinities, forming polyphenols or a collagen peptide-lactoferrin-EGCG ternary complex; for neutral oligosaccharides, the ternary complex was generated mainly through steric effects, accompanied by a change in the lactoferrin secondary structure induced by gallic acid, chlorogenic acid, and xylo-oligosaccharide. Polyphenols, oligosaccharides, or collagen peptide restraining the formation of lactoferrin-EGCG aggregate could be applied in the design of clear products in the food, pharmaceutical, and cosmetic industries.
Wei
W
Yang
Fuguo
F
Liu
Chenqi
C
Xu
Cuixia
C
Sun
Fang
F
Yuan
Yanxiang
Y
Gao
D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry; D003094:Collagen / Q000737:chemistry*; D005503:Food Additives / Q000737:chemistry*; D006801:Humans; D007781:Lactoferrin / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry*; D059808:Polyphenols / Q000737:chemistry*; D066329:Protein Aggregates; D017433:Protein Structure, Secondary
D005503:Food Additives; D009844:Oligosaccharides; D059808:Polyphenols; D066329:Protein Aggregates; D002392:Catechin; D003094:Collagen; C045651:epigallocatechin gallate; D007781:Lactoferrin
(−)-epigallocatechin gallate; aggregation inhibitors; collagen peptide; lactoferrin; oligosaccharides; polyphenols