Non-continuous measurement of intraocular pressure in laboratory animals.
Glaucoma is a leading cause of blindness, which is treatable but currently incurable. Numerous animal models therefore have both been and continue to be utilized in the study of numerous aspects of this condition. One important facet associated with the use of such models is the ability to accurately and reproducibly measure (by cannulation) or estimate (by tonometry) intraocular pressure (IOP). At this juncture there are several different approaches to IOP measurement in different experimental animal species, and the list continues to grow. We feel therefore that a review of this subject matter is timely and should prove useful to others who wish to perform similar measurements. The general principles underlying various types of tonometric and non-tonometric techniques for non-continuous determination of IOP are considered. There follows discussion of specific details as to how these techniques are applied to experimental animal species involved in the research of this disease. Specific comments regarding anesthesia, circadian rhythm, and animal handling are also included, especially in the case of rodents. Brief consideration is also given to possible future developments.
North Texas Eye Research Institute (NTERI), University of North Texas Health Science Center (UNTHSC), 3500 Camp Bowie Boulevard, Fort Worth, TX 76107, USA; Department of Cell Biology and Immunology, University of North Texas Health Science Center (UNTHSC), 3500 Camp Bowie Boulevard, Fort Worth, TX 76107, USA. Electronic address: Cameron.Millar@unthsc.edu.
J Cameron
JC
Millar
North Texas Eye Research Institute (NTERI), University of North Texas Health Science Center (UNTHSC), 3500 Camp Bowie Boulevard, Fort Worth, TX 76107, USA; Department of Cell Biology and Immunology, University of North Texas Health Science Center (UNTHSC), 3500 Camp Bowie Boulevard, Fort Worth, TX 76107, USA; Department of Pharmaceutical Sciences, University of North Texas Health Science Center (UNTHSC), 3500 Camp Bowie Boulevard, Fort Worth, TX 76107, USA.
Iok-Hou
IH
Pang
D000818:Animals; D000830:Animals, Laboratory; D004195:Disease Models, Animal; D005901:Glaucoma / Q000175:diagnosis* / Q000503:physiopathology; D007429:Intraocular Pressure / Q000502:physiology*; D014065:Tonometry, Ocular / Q000379:methods*
Anterior chamber cannulation; Intraocular pressure; Laboratory animals; Posterior chamber cannulation; Servo-null micropipette; Tonometry; Vitreous cannulation