Uric Acid Promotes Osteogenic Differentiation and Inhibits Adipogenic Differentiation of Human Bone Mesenchymal Stem Cells.
To investigate the effect of uric acid on the osteogenic and adipogenic differentiation of human bone mesenchymal stem cells (hBMSCs). The hBMSCs were isolated from bone marrow of six healthy donors. Cell morphology was observed by microscopy and cell surface markers (CD44 and CD34) of hBMSCs were analyzed by immunofluorescence. Cell morphology and immunofluorescence analysis showed that hBMSCs were successfully isolated from bone marrow. The number of hBMSCs in uric acid groups was higher than that in the control group on day 3, 4, and 5. Alizarin red staining showed that number of calcium nodules in uric acid groups was more than that of the control group. Oil red-O staining showed that the number of red fat vacuoles decreased with the increased concentration of uric acid. In summary, uric acid could promote the proliferation and osteogenic differentiation of hBMSCs while inhibit adipogenic differentiation of hBMSCs.
Department of Orthopedics, Shanghai Jiading Central Hospital, Shanghai, 201800, China.
Hui-Zhang
HZ
Li
Department of Orthopedics, Changhai Hospital Affiliated to the Second Military Medical University, Shanghai, 200433, China.
Zhi
Z
Chen
Department of Orthopedics, Changhai Hospital Affiliated to the Second Military Medical University, Shanghai, 200433, China.
Cang-Long
CL
Hou
Department of Orthopedics, Changhai Hospital Affiliated to the Second Military Medical University, Shanghai, 200433, China.
Yi-Xing
YX
Tang
Department of Orthopedics, Changhai Hospital Affiliated to the Second Military Medical University, Shanghai, 200433, China.
Fei
F
Wang
Department of Emergency & Trauma, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 200120, China.
Qing-Ge
QG
Fu
D050156:Adipogenesis / Q000187:drug effects*; D000328:Adult; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D005455:Fluorescent Antibody Technique; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D010012:Osteogenesis / Q000187:drug effects*; D014527:Uric Acid / Q000494:pharmacology*; D055815:Young Adult
D014527:Uric Acid
Adipogenesis; Human Bone Mesenchymal Stem Cells; Osteogenesis; Proliferation; Uric Acid