Generation of a pair of independently binding DNA aptamers in a single round of selection using proximity ligation.
The ability to rapidly generate a pair of aptamers that bind independently to a protein target would greatly extend their use as reagents for two site ('sandwich') assays. We describe here a method to achieve this through proximity ligation. Using lysozyme as a target we demonstrate that under optimal conditions such a pair of aptamers, with nanomolar affinities, can be generated in a single round.
Department of Chemistry, Imperial College London, UK. t.cass@imperial.ac.uk.
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Chumphukam
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Piletsky
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D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000378:metabolism; D015723:Gene Library; D009113:Muramidase / Q000737:chemistry / Q000378:metabolism; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D052156:SELEX Aptamer Technique
D052157:Aptamers, Nucleotide; D009113:Muramidase