A High-Throughput UHPLC-QqQ-MS Method for Polyphenol Profiling in Rosé Wines.
A rapid, sensitive and selective analysis method using Ultra High Performance Liquid Chromatography coupled to triple-quadrupole Mass Spectrometry (UHPLC-QqQ-MS) has been developed for the quantification of polyphenols in rosé wines. The compound detection being based on specific MS transitions in Multiple Reaction Monitoring (MRM) mode, the present method allows the selective quantification of up to 152 phenolic and two additional non-phenolic wine compounds in 30 min without sample purification or pre-concentration, even at low concentration levels. This method was repeatably applied to a set of 12 rosé wines and thus proved to be suitable for high-throughput and large-scale metabolomics studies.
INRA, UMR 1083 Sciences Pour l'Oenologie, Polyphenol Analysis Facility, 2 place Viala, F-34060 Montpellier, France. eniram.lambert@laposte.net.
Marine
M
Lambert
INRA, UMR 1083 Sciences Pour l'Oenologie, Polyphenol Analysis Facility, 2 place Viala, F-34060 Montpellier, France. meudec@supagro.inra.fr.
Emmanuelle
E
Meudec
INRA, UMR 1083 Sciences Pour l'Oenologie, Polyphenol Analysis Facility, 2 place Viala, F-34060 Montpellier, France. verbaere@supagro.inra.fr.
Arnaud
A
Verbaere
INRA, UMR 1083 Sciences Pour l'Oenologie, Polyphenol Analysis Facility, 2 place Viala, F-34060 Montpellier, France. pfp@supagro.inra.fr.
Gérard
G
Mazerolles
INRA, UMR 1083 Sciences Pour l'Oenologie, Polyphenol Analysis Facility, 2 place Viala, F-34060 Montpellier, France. wirth.clap@wanadoo.fr.
Jérémie
J
Wirth
Centre de Recherche et d'Expérimentation sur le Vin Rosé, 70 avenue Wilson, F-83550 Vidauban, France. gilles.masson@vignevin.com.
Gilles
G
Masson
INRA, UMR 1083 Sciences Pour l'Oenologie, Polyphenol Analysis Facility, 2 place Viala, F-34060 Montpellier, France. cheynier@supagro.inra.fr.
Véronique
V
Cheynier
INRA, UMR 1083 Sciences Pour l'Oenologie, Polyphenol Analysis Facility, 2 place Viala, F-34060 Montpellier, France. sommerer@supagro.inra.fr.
Nicolas
N
Sommerer
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D055432:Metabolomics / Q000379:methods; D059808:Polyphenols / Q000737:chemistry*; D027826:Rosa / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods; D014920:Wine / Q000032:analysis*
D059808:Polyphenols