Genetic diversity in oxytocin ligands and receptors in New World monkeys.
Oxytocin (OXT) is an important neurohypophyseal hormone that influences wide spectrum of reproductive and social processes. Eutherian mammals possess a highly conserved sequence of OXT (Cys-Tyr-Ile-Gln-Asn-Cys-Pro-Leu-Gly). However, in this study, we sequenced the coding region for OXT in 22 species covering all New World monkeys (NWM) genera and clades, and characterize five OXT variants, including consensus mammalian Leu(8)-OXT, major variant Pro(8)-OXT, and three previously unreported variants: Ala(8)-OXT, Thr(8)-OXT, and Phe(2)-OXT. Pro(8)-OXT shows clear structural and physicochemical differences from Leu(8)-OXT. We report multiple predicted amino acid substitutions in the G protein-coupled OXT receptor (OXTR), especially in the critical N-terminus, which is crucial for OXT recognition and binding. Genera with same Pro(8)-OXT tend to cluster together on a phylogenetic tree based on OXTR sequence, and we demonstrate significant coevolution between OXT and OXTR. NWM species are characterized by high incidence of social monogamy, and we document an association between OXTR phylogeny and social monogamy. Our results demonstrate remarkable genetic diversity in the NWM OXT/OXTR system, which can provide a foundation for molecular, pharmacological, and behavioral studies of the role of OXT signaling in regulating complex social phenotypes.
Callitrichid Research Centre, Department of Psychology, University of Nebraska at Omaha, Omaha, NE 68182, United States of America; Key Laboratory for Animal Biotechnology of Jiangxi Province and Ministry of Agriculture of China, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China; Department of Biology, University of Nebraska at Omaha, Omaha, NE 68182, United States of America.
Dongren
D
Ren
Department of Biology, University of Nebraska at Omaha, Omaha, NE 68182, United States of America.
Guoqing
G
Lu
School of Biological Sciences, University of Nebraska Lincoln, Lincoln, NE 68588, United States of America.
Hideaki
H
Moriyama
Callitrichid Research Centre, Department of Psychology, University of Nebraska at Omaha, Omaha, NE 68182, United States of America.
Aaryn C
AC
Mustoe
Callitrichid Research Centre, Department of Psychology, University of Nebraska at Omaha, Omaha, NE 68182, United States of America; Department of Biology, University of Nebraska at Omaha, Omaha, NE 68182, United States of America.
Emily B
EB
Harrison
Callitrichid Research Centre, Department of Psychology, University of Nebraska at Omaha, Omaha, NE 68182, United States of America; Department of Biology, University of Nebraska at Omaha, Omaha, NE 68182, United States of America.
Jeffrey A
JA
French
D000595:Amino Acid Sequence; D000818:Animals; D001522:Behavior, Animal; D019143:Evolution, Molecular; D005260:Female; D014644:Genetic Variation; D008297:Male; D008969:Molecular Sequence Data; D010121:Oxytocin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D051078:Platyrrhini / Q000235:genetics*; D054730:Protein Interaction Domains and Motifs; D018045:Receptors, Oxytocin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment
D018045:Receptors, Oxytocin; D010121:Oxytocin