Live Cell MicroRNA Imaging Using Cascade Hybridization Reaction.
Recent advances in RNA research have posed new directives in biology and chemistry to uncover the complex roles of ribonucleic acids in cellular processes. Innovative techniques to visualize native RNAs, particularly, short, low-abundance RNAs in live cells, can dramatically impact current research on the roles of RNAs in biology. Herein, we report a novel method for real-time, microRNA imaging inside live cells based on programmable oligonucleotide probes, which self-assemble through the Cascade Hybridization Reaction (CHR).
‡Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, United States.
Zoya
Z
Cheglakov
†Department of Chemistry, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, United States.
Timothy M
TM
Cronin
‡Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, United States.
Chuan
C
He
†Department of Chemistry, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, United States.
Yossi
Y
Weizmann
D001483:Base Sequence; D002470:Cell Survival; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000032:analysis*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D015345:Oligonucleotide Probes / Q000737:chemistry; D061848:Optical Imaging
D035683:MicroRNAs; D015345:Oligonucleotide Probes