In Vivo Molecular MRI Imaging of Prostate Cancer by Targeting PSMA with Polypeptide-Labeled Superparamagnetic Iron Oxide Nanoparticles.
The prostate specific membrane antigen (PSMA) is broadly overexpressed on prostate cancer (PCa) cell surfaces. In this study, we report the synthesis, characterization, in vitro binding assay, and in vivo magnetic resonance imaging (MRI) evaluation of PSMA targeting superparamagnetic iron oxide nanoparticles (SPIONs). PSMA-targeting polypeptide CQKHHNYLC was conjugated to SPIONs to form PSMA-targeting molecular MRI contrast agents. In vitro studies demonstrated specific uptake of polypeptide-SPIONs by PSMA expressing cells. In vivo MRI studies found that MRI signals in PSMA-expressing tumors could be specifically enhanced with polypeptide-SPION, and further Prussian blue staining showed heterogeneous deposition of SPIONs in the tumor tissues. Taken altogether, we have developed PSMA-targeting polypeptide-SPIONs that could specifically enhance MRI signal in tumor-bearing mice, which might provide a new strategy for the molecular imaging of PCa.
Department of Ultrasound in Medicine, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200092, China. tttmax2004@126.com.
Yunkai
Y
Zhu
State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200032, China. ysun@shsci.org.
Ying
Y
Sun
Department of Ultrasound in Medicine, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200092, China. joychen1266@126.com.
Yaqing
Y
Chen
Department of Ultrasound in Medicine, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200092, China. lwy_ultras@126.com.
Weiyong
W
Liu
Department of Ultrasound in Medicine, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200092, China. tenine@163.com.
Jun
J
Jiang
Department of Pathology, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200092, China. celiceli02@126.com.
Wenbin
W
Guan
Department of Radiology, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200092, China. tttmax2004@hotmail.com.
Zhongyang
Z
Zhang
State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200032, China. yrduan@shsci.org.
Yourong
Y
Duan
D000818:Animals; D000954:Antigens, Surface / Q000737:chemistry*; D045744:Cell Line, Tumor; D003287:Contrast Media / Q000737:chemistry; D005290:Ferric Compounds / Q000737:chemistry*; D005295:Ferrocyanides / Q000737:chemistry; D043425:Glutamate Carboxypeptidase II / Q000737:chemistry*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D008279:Magnetic Resonance Imaging; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D036103:Nanotechnology; D009368:Neoplasm Transplantation; D010455:Peptides / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000473:pathology; D011485:Protein Binding; D015398:Signal Transduction
D000954:Antigens, Surface; D003287:Contrast Media; D005290:Ferric Compounds; D005295:Ferrocyanides; D010455:Peptides; C000499:ferric oxide; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C473848:FOLH1 protein, human; D043425:Glutamate Carboxypeptidase II; C000170:ferric ferrocyanide