Association between lymphocyte antigen receptor gene rearrangements and histopathological evaluation in canine chronic enteropathy.
Although definitive diagnosis of chronic enteropathy (CE) and gastrointestinal (GI) lymphoma requires histopathological evaluation of the GI tract, these conditions are often still difficult to differentiate from each other. Polymerase chain reaction (PCR) for antigen receptor gene rearrangements (PARR) has been applied recently as an adjunctive for diagnosis of lymphoid tumors; however, its clinical value in canine CE and GI lymphoma remains unclear. The purpose of this study was to investigate the relationship between PARR and histopathological diagnosis, degree of enteritis or lymphoma, and long-term prognosis in dogs, in order to evaluate the clinical significance of PARR. Endoscopic biopsy specimens obtained from 96 dogs with chronic enteritis (mild, n=14; moderate, n=20; marked, n=62) and 21 dogs with GI lymphoma were used. Clonality was observed in 51% of the animals with chronic enteritis; interestingly, it was found in 29% of those with only mild enteritis. In dogs with marked enteritis, the rate of PARR was higher in those with lymphocyte epitheliotropism than in those without epitheliotropism. The sensitivity of PARR in animals with GI lymphoma was 76%. There was no significant prognostic difference between chronic enteritis with or without clonal rearrangements. In contrast, dogs histopathologically diagnosed with marked enteritis had a significantly shorter survival time than did those with mild or moderate enteritis. While the significance of PARR in the diagnosis of GI lymphoma remains uncertain, the pathological roles of clonally expanding lymphocytes in canine CE should be investigated further.
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Saaya
S
Hiyoshi
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. Electronic address: aohno@mail.ecc.u-tokyo.ac.jp.
Koichi
K
Ohno
Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Kazuyuki
K
Uchida
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Yuko
Y
Goto-Koshino
Japan Small Animal Medical Center, 2-27-4 Nakatomi-minami, Tokorozawa, Saitama 359-0003, Japan.
Ko
K
Nakashima
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Kenjiro
K
Fukushima
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Hideyuki
H
Kanemoto
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Shingo
S
Maeda
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Hajime
H
Tsujimoto
D000818:Animals; D002908:Chronic Disease; D004283:Dog Diseases / Q000276:immunology / Q000473:pathology*; D004285:Dogs; D004386:Duodenum / Q000473:pathology; D004751:Enteritis / Q000175:diagnosis / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D005260:Female; D005770:Gastrointestinal Neoplasms / Q000175:diagnosis / Q000276:immunology / Q000473:pathology / Q000662:veterinary; D015329:Gene Rearrangement, T-Lymphocyte / Q000276:immunology*; D007413:Intestinal Mucosa / Q000473:pathology; D008223:Lymphoma / Q000175:diagnosis / Q000276:immunology / Q000473:pathology / Q000662:veterinary; D008297:Male; D011379:Prognosis; D012189:Retrospective Studies
Antigen receptor gene rearrangements; Chronic enteritis; Chronic enteropathy; Dog; Histopathology; Intestinal lymphoma