Collagen synthesis promoting pullulan-PEI-ascorbic acid conjugate as an efficient anti-cancer gene delivery vector.
Cationized pullulan (pullulan-PEI; PP) was synthesized and further modified with an anti-oxidant molecule, ascorbic acid (PPAA) at various ratios. The nanoplexes formed at an optimum ratio of 4:1 was within a size of 150nm and had a zeta potential of 9-14mV. The nanoplexes at this ratio was used for further investigations. The cell internalization and transfection efficiency of these nanoplexes were determined in presence of serum. The internalization and transfection efficiency were found to be unaffected by the presence of fetal bovine serum. Another interesting observation was that this polymer was found to have collagen synthesis promoting property. The collagen synthesis effect of these polymers was quantified and observed that PPAA3 promoted the highest. Transfection efficiency was evaluated by assessing the p53 gene expression in C6 rat glioma cells and cell death was quantified to be 96% by flow cytometry, thus establishing the high efficacy of this polymer.
Biosurface Technology Division, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala 695012, India.
Lizebona August
LA
Ambattu
Biosurface Technology Division, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala 695012, India. Electronic address: rekhamr@sctimst.ac.in.
M R
MR
Rekha
D000818:Animals; D001205:Ascorbic Acid / Q000737:chemistry*; D045744:Cell Line, Tumor; D003094:Collagen / Q000737:chemistry; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000008:administration & dosage* / Q000235:genetics; D005910:Glioma / Q000235:genetics / Q000628:therapy; D005936:Glucans / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry; D011094:Polyethyleneimine / Q000737:chemistry*; D051381:Rats; D014162:Transfection / Q000379:methods; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
D005936:Glucans; D016159:Tumor Suppressor Protein p53; C009109:pullulan; D011094:Polyethyleneimine; D003094:Collagen; D004247:DNA; D001205:Ascorbic Acid
Ascorbic acid; Gene delivery; Polyethylene imine; Pullulan; p53