GLUTATHIONE S-TRANSFERASE Genes IN THE RICE LEAFFOLDER, Cnaphalocrocis medinalis (LEPIDOPTERA: PYRALIDAE): IDENTIFICATION AND EXPRESSION PROFILES.
In insects, glutathione S-transferases (GSTs) play critical roles in the detoxification of various insecticides, resulting in insecticide resistance. The rice leaffolder, Cnaphalocrocis medinalis, is an economically important pest of rice in Asia. GST genes have not been largely identified in this insect species. In the present study, by searching the transcriptome dataset, 25 candidate GST genes were identified in C. medinalis for the first time. Of these, 23 predicted GST proteins fell into five cytosolic classes (delta, epsilon, omega, sigma, and zeta), and two were assigned to the "unclassified" subgroup. Real-time quantitative PCR analysis showed that these GST genes were differentially expressed in various tissues, including the midgut, Malpighian tubules, and fat body of larvae, and the antenna, abdomen, and leg of adults, indicating diversified functions for these genes. Transcription levels of CmGSTd2, CmGSTe6, and CmGSTe7 increased significantly in larvae following exposure to chlorpyrifos, suggesting that these GST genes could be involved in the detoxification of this insecticide. The results of our study pave the way to a better understanding of the detoxification system of C. medinalis.
College of Plant Protection, Anhui Agricultural University, Hefei, Anhui, P.R. China.
Su
S
Liu
College of Plant Protection, Anhui Agricultural University, Hefei, Anhui, P.R. China.
Xiang-Jun
XJ
Rao
College of Plant Protection, Anhui Agricultural University, Hefei, Anhui, P.R. China.
Mao-Ye
MY
Li
College of Plant Protection, Anhui Agricultural University, Hefei, Anhui, P.R. China.
Ming-Feng
MF
Feng
College of Plant Protection, Anhui Agricultural University, Hefei, Anhui, P.R. China.
Meng-Zhu
MZ
He
College of Plant Protection, Anhui Agricultural University, Hefei, Anhui, P.R. China.
Shi-Guang
SG
Li
D000818:Animals; D004390:Chlorpyrifos / Q000494:pharmacology; D020869:Gene Expression Profiling; D017344:Genes, Insect; D005982:Glutathione Transferase / Q000235:genetics*; D008658:Inactivation, Metabolic / Q000235:genetics; D007305:Insecticide Resistance / Q000235:genetics; D007306:Insecticides / Q000494:pharmacology; D007814:Larva / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D009036:Moths / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D010802:Phylogeny; D059467:Transcriptome
D007306:Insecticides; D005982:Glutathione Transferase; D004390:Chlorpyrifos
Cnaphalocrocis medinalis; differential gene expression; glutathione S-transferases (GST); insecticide detoxification