Mapping the CXCR4 receptor on breast cancer cells.
The CXCR4 receptor triggers cell migration and, in breast cancer, promotes metastasis. To date, the dynamic assembly of CXCR4 on the cell surface as a mediator of receptor binding is not well characterized. The objective of this work is to quantify the density, spatial organization, and magnitude of binding of the CXCR4 receptor on live metastatic breast cancer (MBC) cells. We measured the Young's modulus, the CXCR4 surface density, and CXCR4 unbinding force on MBC cells by atomic force microscopy. We conclude that the CXCR4 density, spatial organization, and matrix stiffness are paramount to achieve strong binding.
Department of Biomedical Engineering, The City College of New York, 160 Convent Avenue, New York, NY 10031, United States.
Biran
B
Wang
Department of Biomedical Engineering, The City College of New York, 160 Convent Avenue, New York, NY 10031, United States; Vascular Biology Program, Boston Children's Hospital, 300 Longwood Avenue, Boston, MA 02115, United States; Department of Surgery, Harvard Medical School and Boston Children's Hospital, 300 Longwood Avenue, Boston, MA 02115, United States.
Peng
P
Guo
Department of Biomedical Engineering, The City College of New York, 160 Convent Avenue, New York, NY 10031, United States. Electronic address: dauguste@ccny.cuny.edu.
Debra T
DT
Auguste
D001940:Breast / Q000378:metabolism / Q000473:pathology*; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D055119:Elastic Modulus; D005260:Female; D006801:Humans; D018625:Microscopy, Atomic Force; D009362:Neoplasm Metastasis / Q000473:pathology*; D019718:Receptors, CXCR4 / Q000032:analysis* / Q000378:metabolism
D019718:Receptors, CXCR4
Adhesion; Atomic force microscopy (AFM); Breast cancer; CXCR4; Drug delivery; Young's modulus