Anti-apoptotic effect of microRNA-30b in early phase of rat myocardial ischemia-reperfusion injury model.
This study aimed to investigate the effect of microRNA-30b (miR-30b) in rat myocardial ischemic-reperfusion (I/R) injury model. We randomly divided Sprague-Dawley (SD) rats (n = 80) into five groups: 1) control group; 2) miR-30b group; 3) sham-operated group; 4) I/R group, and 5) I/R+miR-30b group. Real-time quantitative polymerase chain reaction, immunohistochemical staining and Western blot analysis were conducted. TUNEL assay was employed for testing cardiomyocyte apoptosis. Our results showed that miR-30b levels were down-regulated in I/R group and I/R + miR-30b group compared with sham-operated group (both P < 0.05). However, miR-30b level in I/R + miR-30b group was higher than I/R group (P < 0.05). Markedly, the apoptotic rate in I/R group showed highest in I/R group (P < 0.05). Additionally, the results illustrated that protein levels of Bcl-2, Bax, and caspase-3 were at higher levels in ischemic regions in I/R group, comparing to sham-operated group (all P < 0.05), while Bcl-2/Bax was reduced (P < 0.05). Bcl-2 level and Bcl-2/Bax were obviously increased in I/R + miR-30b group by comparison with I/R group, and expression levels of Bax and caspase-3 were down-regulated (all P < 0.05). We also found that in I/R + miR-30b group, KRAS level was apparently lower and p-AKT level was higher by comparing with I/R group (both P < 0.05). Our study indicated that miR-30b overexpression had anti-apoptotic effect on early phase of rat myocardial ischemia injury model through targeting KRAS and activating the Ras/Akt pathway.
Department of Cardiology, the Second Hospital of Jilin University, Changchun, 130041, P. R. China.
Chun-Li
CL
Song
Department of Cardiology, the Second Hospital of Jilin University, Changchun, 130041, P. R. China.
Bin
B
Liu
Department of Cardiology, the Second Hospital of Jilin University, Changchun, 130041, P. R. China.
Jin-Peng
JP
Wang
Department of Physiology, the College of Basic Medical Sciences of Jilin University, Changchun, 130021, P. R. China.
Bei-Lin
BL
Zhang
Department of Cardiology, the Second Hospital of Jilin University, Changchun, 130041, P. R. China.
Ji-Chang
JC
Zhang
Department of Clinical Laboratory, the Second Hospital of Jilin University, Changchun, 130041, P. R. China.
Li-Yan
LY
Zhao
Department of Cardiology, the Second Hospital of Jilin University, Changchun, 130041, P. R. China.
Yong-Feng
YF
Shi
Department of Cardiology, the Second Hospital of Jilin University, Changchun, 130041, P. R. China.
Yang-Xue
YX
Li
Department of Cardiology, the Second Hospital of Jilin University, Changchun, 130041, P. R. China.
Guan
G
Wang
Department of Cardiology, the Second Hospital of Jilin University, Changchun, 130041, P. R. China.
Hong-Ying
HY
Diao
Department of Cardiology, the Second Hospital of Jilin University, Changchun, 130041, P. R. China.
Qian
Q
Li
Department of Cardiology, the Second Hospital of Jilin University, Changchun, 130041, P. R. China.
Xin
X
Xue
Department of Cardiology, the Second Hospital of Jilin University, Changchun, 130041, P. R. China.
Jun-Duo
JD
Wu
Department of Cardiology, the Second Hospital of Jilin University, Changchun, 130041, P. R. China.
Jia
J
Liu
Department of Cardiology, the Second Hospital of Jilin University, Changchun, 130041, P. R. China.
Yun-Peng
YP
Yu
Department of Cardiology, the Second Hospital of Jilin University, Changchun, 130041, P. R. China.
Dan
D
Cai
Department of Cardiology, the Second Hospital of Jilin University, Changchun, 130041, P. R. China.
Zhi-Xian
ZX
Liu
D000818:Animals; D017209:Apoptosis; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D017202:Myocardial Ischemia / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D015428:Myocardial Reperfusion Injury / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction
C556157:MIRN30 microRNA, rat; D035683:MicroRNAs; D019253:Proto-Oncogene Proteins c-bcl-2; C505409:Casp3 protein, mouse; D053148:Caspase 3; C495971:Hras protein, mouse; D016283:Proto-Oncogene Proteins p21(ras)
ANTI-APOPTOSIS; BAX; BCL-2; CASPASE-3; ISCHEMIC-REPERFUSION INJURY; KRAS; MICRORNA-30B; RAS/AKT PATHWAY