Nitric Oxide Plays a Central Role in Water Stress-Induced Tanshinone Production in Salvia miltiorrhiza Hairy Roots.
Nitric oxide (NO), a well-known signaling molecule plays an important role in abiotic and biotic stress-induced production of plant secondary metabolites. In this study, roles of NO in water stress-induced tanshinone production in Salvia miltiorrhiza hairy roots were investigated. The results showed that accumulations of four tanshinone compounds in S. miltiorrhiza hairy roots were significantly stimulated by sodium nitroprusside (SNP, a NO donor) at 100 μM. Effects of SNP were just partially arrested by the mevalonate (MVA) pathway inhibitor (mevinolin), but were completely inhibited by the 2-C-methyl-d-erythritol-4-phosphate pathway (MEP) inhibitor (fosmidomycin). The increase of tanshinone accumulation and the up-regulation of HMGR and DXR expression by PEG and ABA treatments were partially inhibited by an inhibitor of NO biosynthesis (Nω-nitro-L-arginine methyl ester (L-NAME)) and a NO scavenger (2-(4-Carboxyphenyl)- 4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO)). Simultaneously, NO generation in the hairy roots was triggered by PEG and ABA, and the effects were also arrested by c-PTIO and L-NAME. These results indicated that NO signaling probably plays a central role in water stress-induced tanshinone production in S. miltiorrhiza hairy roots. SNP mainly stimulated the MEP pathway to increase tanshinone accumulation.
College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China. ydf807@163.com.
Xuhong
X
Du
College of Biological Science & Engineering, Shaanxi University of Technology, Hanzhong 723000, China. chenluzhang@126.com.
Chenlu
C
Zhang
College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China. gwl1016@aliyun.com.
Wanli
W
Guo
College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China. jwb@zstu.edu.cn.
Weibo
W
Jin
College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China. liangzs@ms.iswc.ac.cn.
Zongsuo
Z
Liang
Tasly R&D Institute, Tasly Holding Group Co. Ltd, Tianjin 300410, China. yxj@tasly.com.
Xijun
X
Yan
Tasly R&D Institute, Tasly Holding Group Co. Ltd, Tianjin 300410, China. guozx@tasly.com.
Zhixin
Z
Guo
Tianjin Tasly Modern TCM Resources Co., Ltd., Tianjin 300402, China. lyzc596392@163.com.
Yan
Y
Liu
College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China. ydf807@sina.com.|Tasly R&D Institute, Tasly Holding Group Co. Ltd, Tianjin 300410, China. ydf807@sina.com.
Dongfeng
D
Yang
D045784:Abietanes / Q000096:biosynthesis*; D000040:Abscisic Acid / Q000737:chemistry / Q000378:metabolism; D003681:Dehydration / Q000378:metabolism*; D055864:Droughts; D004896:Erythritol / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors; D005578:Fosfomycin / Q000031:analogs & derivatives / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D008148:Lovastatin / Q000494:pharmacology; D008798:Mevalonic Acid / Q000378:metabolism; D019331:NG-Nitroarginine Methyl Ester / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D009599:Nitroprusside / Q000494:pharmacology; D018517:Plant Roots / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000378:metabolism; D027561:Salvia miltiorrhiza / Q000378:metabolism*; D015398:Signal Transduction; D013403:Sugar Phosphates / Q000037:antagonists & inhibitors
C114232:2-C-methylerythritol 4-phosphate; D045784:Abietanes; D013403:Sugar Phosphates; C021751:tanshinone; D009599:Nitroprusside; D005578:Fosfomycin; D009569:Nitric Oxide; D011092:Polyethylene Glycols; C024640:fosmidomycin; D000040:Abscisic Acid; D008148:Lovastatin; D004896:Erythritol; D008798:Mevalonic Acid; D019331:NG-Nitroarginine Methyl Ester