Relationship between CATSPERB, NR5A2 gene polymorphisms and Peak Bone Mineral Density in College Students in China.
BACKGROUND
Peak bone mineral density (PBMD) is influenced by both genetic and environmental factors, genes explains most of variation. As the novel candidate genes CATSPERB and NR5A2 may have been associated with spinal PBMD in adult. This study was to investigate the relationship among these two genes^ PBMD and the life style factors in young female.


METHODS
The rs1298989 single nucleotide polymorphism (SNP) of the CATSPERB gene and the rs3762397 SNP of the NR5A2 gene were genotyped using SNaPshot® in 359 students from Xinjiang. The prospective study included 203 Han and 156 Uyghur subjects. PBMD was measured using quantitative computed tomography (QCT). Calcium, phosphate and alkaline phosphatase were measured by ELISA method. Physical activity, dietary calcium and life styles were assessed by questionnaire.


RESULTS
Both SNPs showed differences in genotype and allele frequencies (P < 0.05) between the Han and Uyghur subjects. Total calcium intake, energy intake, tea and milk intake were also significantly different between two groups (P < 0.05). Multiple regression analysis showed an association between PBMD and vitamin D intake (P = 0.000), milk (P = 0.000), exercise (P = 0.029), rs1298989 (P = 0.028), energy intake (P = 0.043).


CONCLUSIONS
This study demonstrated the polymorphisms of the rs1298989 and rs3762397 are associated with PBMD both in Han and Uyghur subjects. PBMD, in Xinjiang, appears to be associated with several known factors that are well described in the literature. While the genotypes of rs1298989 and rs3762397 do not appear have a strong effect on the PBMD.
1. Imaging Center, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054 , Xinjiang, China.
Yuan
Y
Zhao
1. Imaging Center, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054 , Xinjiang, China.
Wenya
W
Liu
1. Imaging Center, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054 , Xinjiang, China.
Ma
M
Hua
2. Urumqui Center for Disease Control and Prevention, Urumqi 830011 , Xinjiang, China.
Raoni
R
Shi
1. Imaging Center, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054 , Xinjiang, China.
Haitao
H
Wang
1. Imaging Center, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054 , Xinjiang, China.
Wen
W
Yang
CATSPERB,; Life styles; Peak bone mineral density (PBMD); rs1298989 NR5A2,; rs3762397