Association of oxidative stress biomarkers with gestational diabetes mellitus in pregnant women: a case-control study.
OBJECTIVE
The relationship between gestational diabetes mellitus (GDM) and oxidative stress has not been fully elucidated. This study examined the association between biomarkers of oxidative stress and GDM.


METHODS
We conducted a case-control study which included 36 women presenting with GDM and 36 asymptomatic matched control subjects who visited Guangzhou Women and Children's Medical Centre, China, from June 2012 to December 2012. Pregnant women were prospectively recruited to the study, and blood samples were collected at the time of a routine oral glucose tolerance test. These samples were then analyzed for levels of endocrine and surrogate markers of oxidative stress.


RESULTS
Compared to control subjects, women with GDM exhibited elevated values for plasma glucose, insulin, and insulin resistance (IR), and showed reduced HOMA pancreatic β-cell function (HOMA-B), insulin sensitivity index (ISI), insulinogenic index, and corrected insulin response at 24-28 weeks gestation. A bivariate logistic regression analysis showed that levels of high-sensitivity C reactive protein (hs-CRP) and high fluorescence reticulocytes at fasting, and hs-CRP in a 1-h OGTT, were significantly associated with GDM. A linear regression analysis showed that levels of hs-CRP (P = 0.003) and reticulocytes (P = 0.029) at fasting were associated with IR, and levels of hs-CRP (P = 0.002) and monocytes (P = 0.006) in a 1-h OGTT were associated with ISI.


CONCLUSIONS
Pregnant women with GDM developed a pathological IR and exhibited β-cell dysfunction. Their decreased ability to compensate for oxidative stress was associated with increased IR and a reduced ISI, which might be important factors in GDM.
Department of Preventive Medicine, School of Public Health, Guangzhou Medical University, Guangzhou, 510182, China.
Chunyan
C
Zhu
Department of Laboratory, Guangzhou Women and Children's Medical Centre, Guangzhou Medical University, Guangzhou, 510623, China.
Hongling
H
Yang
Guangdong General Hospital, Guangdong Academy of Medical Science, Guangzhou, 510080, China.
Qingshan
Q
Geng
Department of Laboratory, Guangzhou Women and Children's Medical Centre, Guangzhou Medical University, Guangzhou, 510623, China.
Qingling
Q
Ma
Department of Laboratory, Guangzhou Women and Children's Medical Centre, Guangzhou Medical University, Guangzhou, 510623, China.
Yan
Y
Long
Department of Preventive Medicine, School of Public Health, Guangzhou Medical University, Guangzhou, 510182, China.
Cheng
C
Zhou
Department of Preventive Medicine, School of Public Health, Guangzhou Medical University, Guangzhou, 510182, China.
Ming
M
Chen
D000328:Adult; D015415:Biomarkers; D001786:Blood Glucose / Q000032:analysis; D002097:C-Reactive Protein / Q000032:analysis; D016022:Case-Control Studies; D016640:Diabetes, Gestational / Q000097:blood* / Q000378:metabolism; D005260:Female; D005951:Glucose Tolerance Test; D006801:Humans; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D018384:Oxidative Stress; D011247:Pregnancy; D055815:Young Adult
D015415:Biomarkers; D001786:Blood Glucose; D007328:Insulin; D002097:C-Reactive Protein