Polyphasic characterization of four new plant pathogenic Phyllosticta species from China, Japan, and the United States.
The black rot disease of Vitis species and other host genera of Vitacease is caused by Phyllosticta ampelicida and allied taxa which is considered to be a species complex. In this paper, we introduce four new species of Phyllosticta, including two from the P. ampelicida complex, based on a polyphasic characterization including disease symptoms and host association, morphology, and molecular phylogeny. The phylogenetic analysis was conducted based on the ribosomal internal transcribed spacer (ITS) region and a combined multi-locus alignment of the ITS, actin (ACT), partial translation elongation factor 1-alpha (TEF-1), and glyceraldehydes 3-phosphate dehydrogenase (GPDH) gene regions. Our study confirms the phylogenetic distinctions of the four new species, as well as their phenotypic differences with known species in the genus.
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China; University of Chinese Academy of Sciences, Shijingshan Rd, Shijingshan, Beijing 100049, PR China.
Nan
N
Zhou
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Qian
Q
Chen
Department of Biology, University of Oregon, Eugene, OR 97403-1210, USA.
George
G
Carroll
Department of Plant Biology and Pathology, Department of Biochemistry and Microbiology, Rutgers University, 59 Dudley Road, Foran Hall 201, New Brunswick, NJ 08901, USA.
Ning
N
Zhang
Department of Agriculture, Fisheries and Forestry, Plant Pathology Herbarium (BRIP), Plant Biosecurity Science, GPO Box 267, Brisbane, QLD 4001, Australia.
Roger G
RG
Shivas
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China. Electronic address: mrcailei@gmail.com.
Lei
L
Cai
D001203:Ascomycota / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D002681:China; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D007564:Japan; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D014481:United States; D027843:Vitis / Q000382:microbiology*
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer
Cryptic species; Guignardia bidwellii; Morphology; Phylogeny; Systematics