Increasing Vero viable cell densities for yellow fever virus production in stirred-tank bioreactors using serum-free medium.
In this work, changes in Vero cell cultivation methods have been employed in order to improve cell growth conditions to obtain higher viable cell densities and to increase viral titers. The propagation of the 17DD yellow fever virus (YFV) in Vero cells grown on Cytodex I microcarriers was evaluated in 3-L bioreactor vessels. Prior to the current changes, Vero cells were repeatedly displaying insufficient microcarrier colonization. A modified cultivation process with four changes has resulted in higher cell densities and higher virus titers than previously observed for 17DD YFV.
Bio-Manguinhos-FIOCRUZ, Technological Development Vice-directory, Rio de Janeiro, RJ, Brazil.
Diogo A
DA
Mattos
Bio-Manguinhos-FIOCRUZ, Technological Development Vice-directory, Rio de Janeiro, RJ, Brazil.
Marlon V
MV
Silva
Bio-Manguinhos-FIOCRUZ, Technological Development Vice-directory, Rio de Janeiro, RJ, Brazil. Electronic address: lgaspar@bio.fiocruz.br.
Luciane P
LP
Gaspar
Federal University of Rio de Janeiro (UFRJ), COPPE, Chemical Engineering Program, Rio de Janeiro, RJ, Brazil.
Leda R
LR
Castilho
D000818:Animals; D019149:Bioreactors; D002452:Cell Count; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D002470:Cell Survival; D002522:Chlorocebus aethiops; D016895:Culture Media, Serum-Free; D014709:Vero Cells; D019562:Viral Load; D014776:Virus Cultivation / Q000379:methods*; D015005:Yellow fever virus / Q000254:growth & development*
D016895:Culture Media, Serum-Free
Bioreactors; Serum-free medium; Vero cells; Yellow fever virus production