Structural characterization of a broccoli polysaccharide and evaluation of anti-cancer cell proliferation effects.
Broccoli is a widely consumed vegetable with abundant amount of nutrients, which bring numerous beneficial effects on human health. The structural information of water-soluble polysaccharides in broccoli was eludicated for the first time in this work. A purified polysaccharide fraction (BPCa) was obtained by column chromatography. It comprised of arabinose (Ara), galactose (Gal), rhamnose (Rha) with a molar ratio of 5.3:0.8:1.0. Nuclear magnetic resonnance spectra data revealed that α-L-1,5-Araf and α-L-1,3,5-Araf are present in the backbone, while α-L-Araf terminal was attended in side chain. α-L-1,2-Rhap was found to be linked to α-L-1,5-Araf in heteronuclear multiple bond correlation spectra. The presences of β-D-1,4-Galp and α-D-1,4-GalpA were also detected. Furthermore, BPCa showed significant anti-cancer cell proliferation activities against HepG2, Siha and MDA-MB-231 carcinoma cell lines. The results indicated that BPCa had a good potential to be applied as functional food additives.
College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 210043, China. Electronic address: xls@zjnu.edu.cn.
Lishan
L
Xu
College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 210043, China.
Jingjing
J
Cao
College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 210043, China.
Wenrong
W
Chen
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000494:pharmacology*; D001089:Arabinose / Q000737:chemistry / Q000494:pharmacology; D001937:Brassica / Q000737:chemistry*; D002240:Carbohydrate Sequence; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D005690:Galactose / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000188:drug therapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D012210:Rhamnose / Q000737:chemistry / Q000494:pharmacology
D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D011134:Polysaccharides; D001089:Arabinose; D012210:Rhamnose; D005690:Galactose
Anti-cancer cell proliferation activity; Broccoli; NMR; Polysaccharide; Structure