Continued progress in developing the Pig-a gene mutation assay.
The Pig-a assay has shown promise as a regulatory assay for evaluating in vivo gene mutation. A recent International Workshop on Genotoxicity Testing workgroup discussed the state of the assay and identified several knowledge gaps in assay development. This Mutagenesis Special Topic includes a collection of reports that addresses some of these knowledge gaps, including identifying the mutations responsible for the Pig-a mutant phenotype, the effect of sex on the response, probing the robustness of the assay and expanding the number of agents tested in the assay, especially agents expected to yield negative responses.
U.S. Food and Drug Administration/National Center for Toxicological Research, Division of Genetic and Molecular Toxicology, Jefferson, AR 72079, USA robert.heflich@fda.hhs.gov.
Robert H
RH
Heflich
U.S. Food and Drug Administration/National Center for Toxicological Research, Division of Genetic and Molecular Toxicology, Jefferson, AR 72079, USA.
Vasily N
VN
Dobrovolsky
Litron Laboratories, Rochester, NY 14623, USA.
Stephen D
SD
Dertinger
D000818:Animals; D058851:GPI-Linked Proteins / Q000096:biosynthesis; D017930:Genes, Reporter; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D016296:Mutagenesis; D009154:Mutation
D058851:GPI-Linked Proteins; D008565:Membrane Proteins; C080086:phosphatidylinositol glycan-class A protein