Arrhenius activation energy of damage to catalase during spray-drying.
The inactivation of catalase during spray-drying over a range of outlet gas temperatures could be closely represented by the Arrhenius equation. From this an activation energy for damage to the catalase could be calculated. The close fit to Arrhenius suggests that the thermally-induced part of inactivation of the catalase during the complex drying and particle-formation processes takes place at constant temperature. These processes are rapid compared with the residence time of the powder in the collecting vessel of the cyclone where dried catalase is exposed to a constant temperature equal to approximately the drying gas outlet temperature. A lower activation energy after spray drying with the ultrasonic nozzle was found than with the 2-fluid nozzle under otherwise identical spray drying conditions. It is feasible that the ultrasonic nozzle when mounted in the lid of the spray dryer heats up toward the drying gas inlet temperature much more that the air-cooled 2-fluid nozzle. Calculation of the Arrhenius activation energy also showed how the stabilizing efficacy of trehalose and mannitol on the catalase varies in strength across the range of drying gas inlet and outlet temperatures examined.
Division of Pharmaceutics, Friedrich-Alexander-University, Erlangen, Germany.
Joachim
J
Schaefer
Division of Pharmaceutics, Friedrich-Alexander-University, Erlangen, Germany. Electronic address: geoff.lee@fau.de.
Geoffrey
G
Lee
D002374:Catalase / Q000737:chemistry*; D003890:Desiccation; D008353:Mannitol / Q000737:chemistry; D013678:Technology, Pharmaceutical; D013696:Temperature; D014199:Trehalose / Q000737:chemistry
D008353:Mannitol; D014199:Trehalose; D002374:Catalase
Activation energy; Arrhenius; Protein; Spray dry; Stabilization