Identification of a Novel Rat NR2B Subunit Gene Promoter Region Variant and Its Association with Microwave-Induced Neuron Impairment.
Microwave radiation has been implicated in cognitive dysfunction and neuronal injury in animal models and in human investigations; however, the mechanism of these effects is unclear. In this study, single nucleotide polymorphism (SNP) sites in the rat GRIN2B promoter region were screened. The associations of these SNPs with microwave-induced rat brain dysfunction and with rat pheochromocytoma-12 (PC12) cell function were investigated. Wistar rats (n = 160) were exposed to microwave radiation (30 mW/cm(2) for 5 min/day, 5 days/week, over a period of 2 months). Screening of the GRIN2B promoter region revealed a stable C-to-T variant at nucleotide position -217 that was not induced by microwave exposure. The learning and memory ability, amino acid contents in the hippocampus and cerebrospinal fluid, and NR2B expression were then investigated in the different genotypes. Following microwave exposure, NR2B protein expression decreased, while the Glu contents in the hippocampus and CSF increased, and memory impairment was observed in the TT genotype but not the CC and CT genotypes. In PC12 cells, the effects of the T allele were more pronounced than those of the C allele on transcription factor binding ability, transcriptional activity, NR2B mRNA, and protein expression. These effects may be related to the detrimental role of the T allele and the protective role of the C allele in rat brain function and PC12 cells exposed to microwave radiation.
Laboratory of Experimental Pathology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing, 100850, China.
Li-Feng
LF
Wang
Vestibular Laboratory, Institute of Aviation Medicine, 28 Fucheng Road, Beijing, 100142, China.|Department of Aerospace Medicine Aerospace Biodynamics, The Fourth Military Medical University, 169 Changlexi Road, Xian, 100032, China.
Da-Wei
DW
Tian
Laboratory of Experimental Pathology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing, 100850, China.
Hai-Juan
HJ
Li
Laboratory of Experimental Pathology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing, 100850, China.
Ya-Bing
YB
Gao
Laboratory of Experimental Pathology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing, 100850, China.
Chang-Zhen
CZ
Wang
Laboratory of Experimental Pathology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing, 100850, China.
Li
L
Zhao
Laboratory of Experimental Pathology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing, 100850, China.
Hong-Yan
HY
Zuo
Laboratory of Experimental Pathology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing, 100850, China.
Ji
J
Dong
Laboratory of Experimental Pathology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing, 100850, China.
Si-Mo
SM
Qiao
Laboratory of Experimental Pathology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing, 100850, China.
Yong
Y
Zou
Laboratory of Experimental Pathology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing, 100850, China.
Lu
L
Xiong
Radiation Protection, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing, 100850, China.
Hong-Mei
HM
Zhou
Experimental Hematology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing, 100850, China.
Yue-Feng
YF
Yang
Laboratory of Experimental Pathology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing, 100850, China. pengry@nic.bmi.ac.cn.
Rui-Yun
RY
Peng
Laboratory of Experimental Pathology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing, 100850, China. xjhu2003@126.com.
Xiang-Jun
XJ
Hu
D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000473:pathology; D049109:Cell Proliferation; D005787:Gene Frequency / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D008297:Male; D008872:Microwaves; D009474:Neurons / Q000473:pathology*; D016716:PC12 Cells; D011401:Promoter Regions, Genetic; D021122:Protein Subunits / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D016194:Receptors, N-Methyl-D-Aspartate / Q000235:genetics*
C121001:NR2B NMDA receptor; D021122:Protein Subunits; D016194:Receptors, N-Methyl-D-Aspartate
Association; Microwave; NR2B; Neurons; Polymorphism; Promoter