Biomineralized biomimetic organic/inorganic hybrid hydrogels based on hyaluronic acid and poloxamer.
A biomineralized hydrogel system containing hyaluronic acid (HA) and poloxamer composed of a poly(ethylene oxide)/poly(propylene oxide)/poly(ethylene oxide) (PEO-PPO-PEO) block copolymer was developed as a biomimetic thermo-responsive injectable hydrogel system for bone regeneration. Using HA and poloxamer macromers with polymerizable residues, organic/inorganic HA/poloxamer hydrogels with various compositions were prepared and subjected to a biomineralization process to mimic the bone extracellular matrix. An increase in HA content within the hydrogels enhanced intermolecular chelation with calcium ions, leading to an increase in nucleation and growth of calcium phosphate in the hydrogels. After the biomineralization procedure, a crystalline formation was observed within and on the surface of the hydrogel. All of the HA/poloxamer hydrogel samples exhibited relatively high water content of greater than 90% at 25 °C, and the water content was influenced by the HA/poloxamer composition, biomineralization, and temperature. In particular, the HA/poloxamer hydrogel was injectable through a syringe without demonstrating appreciable macroscopic fracture at room temperature, whereas it was more opaque and adopted a more rigid structure as the temperature increased because of the increasing hydrophobicity of poloxamer. The enzymatic degradation behavior of the hydrogels depended on the concentration of hyaluronidase, HA/poloxamer composition, and biomineralization. The release kinetics of model drugs from HA/poloxamer hydrogels was primarily dependent on the drug loading content, water content, biomineralization of the hydrogels, and ionic properties of the drug. These results indicate that biomineralized HA/poloxamer hydrogel is a promising candidate material for a biomimetic hydrogel system that promotes bone tissue repair and regeneration via local delivery of drugs.
College of Pharmacy, Chungnam National University, Daejeon 305-764, South Korea.
Hyun Wook
HW
Huh
Graduate School of Energy Science and Technology, Chungnam National University, Daejeon 305-764, South Korea.
Linlin
L
Zhao
Graduate School of Energy Science and Technology, Chungnam National University, Daejeon 305-764, South Korea; Department of Chemical Engineering Education, College of Education, Chungnam National University, Daejeon 305-764, South Korea. Electronic address: kimsy@cnu.ac.kr.
So Yeon
SY
Kim
D032701:Biomimetics; D018786:Bone Substitutes / Q000008:administration & dosage / Q000737:chemistry* / Q000378:metabolism; D004337:Drug Carriers / Q000008:administration & dosage / Q000737:chemistry* / Q000378:metabolism; D065546:Drug Liberation; D006801:Humans; D006820:Hyaluronic Acid / Q000008:administration & dosage / Q000737:chemistry* / Q000378:metabolism; D006821:Hyaluronoglucosaminidase / Q000378:metabolism; D020100:Hydrogels / Q000008:administration & dosage / Q000737:chemistry* / Q000378:metabolism; D007267:Injections; D008958:Models, Molecular; D020442:Poloxamer / Q000008:administration & dosage / Q000737:chemistry* / Q000378:metabolism; D011092:Polyethylene Glycols / Q000008:administration & dosage / Q000737:chemistry* / Q000378:metabolism; D011409:Propylene Glycols / Q000008:administration & dosage / Q000737:chemistry* / Q000378:metabolism; D013696:Temperature
D018786:Bone Substitutes; D004337:Drug Carriers; D020100:Hydrogels; C116176:PEO-PPO-PEO; D011409:Propylene Glycols; D020442:Poloxamer; D011092:Polyethylene Glycols; D006820:Hyaluronic Acid; D006821:Hyaluronoglucosaminidase
Biomineralization; Hyaluronic acid; Hydrogel; Poloxamer