Callus Growth Kinetics of Physic Nut (Jatropha curcas L.) and Content of Fatty Acids from Crude Oil Obtained In Vitro.
The callus growth kinetics allows identifying the appropriate moment for callus pealing and monitoring the accumulation of primary and secondary metabolites. The physic nut (Jatropha curcas L.) is a plant species used for biofuel production due to its high oil content; however, this plant presents a great amount of bioactive compounds which can be useful for industry. The aim of this research was to establish a calli growth curve and to evaluate the fatty acid profile of crude oil extracted from callus. The callus growth kinetics presented a sigmoid standard curve with six distinct phases: lag, exponential, linear, deceleration, stationary, and decline. Total soluble sugars were higher at the inoculation day. Reducing sugars were higher at the inoculation day and at the 80th day. The highest percentage of ethereal extract (oil content) was obtained at the 120th day of culture, reaching 18 % of crude oil from the callus. The calli produced medium-chain and long-chain fatty acids (from 10 to 18 carbon atoms). The palmitic acid was the fatty acid with the highest proportion in oil (55.4 %). The lipid profile obtained in callus oil was different from the seed oil profile.
Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Curitiba, Brazil.
Jefferson
J
da Luz Costa
André Luís Lopes
AL
da Silva
Mário César Jucoski
MC
Bier
Gilvano Ebling
GE
Brondani
André Luiz
AL
Gollo
Luiz Alberto Junior
LA
Letti
Eduardo Andrea Lemus
EA
Erasmo
Carlos Ricardo
CR
Soccol
D000596:Amino Acids / Q000378:metabolism; D050260:Carbohydrate Metabolism; D005227:Fatty Acids / Q000737:chemistry* / Q000378:metabolism*; D031286:Jatropha / Q000166:cytology* / Q000378:metabolism*; D007700:Kinetics; D010578:Petroleum / Q000032:analysis*; D010940:Plant Proteins / Q000378:metabolism; D012995:Solubility
D000596:Amino Acids; D005227:Fatty Acids; D010578:Petroleum; D010940:Plant Proteins