Role of PTEN in neutrophil extracellular trap formation.
NETosis has been associated with a particular mode of cell death although it is still controversial as to what extent autophagy is involved in NETosis. Class I/AKT/mTOR pathway is a key regulator of autophagy. PTEN tumor suppressor gene encodes a dual specificity phosphatase that antagonizes the phosphatidylinositol 3-kinase in class the I/AKT/mTOR pathway. In this study, we investigated the effects of PTEN down-regulation as well as overexpression on NETosis. Our results show that 35% of HL-60 differentiated neutrophil-like cells generated NETs by PMA. The portion of the population that produced NETs in PTEN knockdown HL-60 differentiated neutrophils was 9% and in PTEN overexpressed HL-60 differentiated neutrophils, it was 56%. Our results show that increasing PTEN expression increases NETs formation in neutrophils, and its suppression reduces NETs.
Department of Medical Genetics, Iran University of Medical Sciences, Tehran, Iran; Department of Human Genetics, Tehran University of Medical Sciences, Tehran, Iran; Pediatrics Infectious Diseases Research Center, Department of Infectious Diseases, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: teimourian.sh@iums.ac.ir.
Shahram
S
Teimourian
Department of Human Genetics, Tehran University of Medical Sciences, Tehran, Iran; Pediatrics Infectious Diseases Research Center, Department of Infectious Diseases, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran; Department of Microbiology and Immunology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran.
Ehsan
E
Moghanloo
D016923:Cell Death / Q000276:immunology; D002454:Cell Differentiation; D058534:Class I Phosphatidylinositol 3-Kinases / Q000235:genetics / Q000276:immunology; D065206:Extracellular Traps / Q000235:genetics / Q000276:immunology*; D005786:Gene Expression Regulation; D018922:HL-60 Cells; D006801:Humans; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology* / Q000473:pathology; D051059:PTEN Phosphohydrolase / Q000819:agonists / Q000037:antagonists & inhibitors / Q000235:genetics / Q000276:immunology*; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000276:immunology; D034741:RNA, Small Interfering / Q000235:genetics / Q000276:immunology; D015398:Signal Transduction; D058570:TOR Serine-Threonine Kinases / Q000235:genetics / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D034741:RNA, Small Interfering; C546842:MTOR protein, human; D058534:Class I Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D058570:TOR Serine-Threonine Kinases; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human; D013755:Tetradecanoylphorbol Acetate
Autophagy; NETosis; Neutrophil extracellular traps; PTEN