Role of heteroplasmic mutations in the mitochondrial genome and the ID4 gene promoter methylation region in the pathogenesis of chronic aplastic anemia in patients suffering from Kidney yin deficiency.
OBJECTIVE
To analyze changes in gene amplification in the mitochondrial genome and in the ID4 gene promoter methylation region in patients with chronic aplastic anemia (CAA) suffering from Kidney (Shen) yin deficiency or Kidney yang deficiency.


METHODS
Bone marrow and oral epithelium samples were collected from CAA patients with Kidney yin deficiency or Kidney yang deficiency (20 cases). Bone marrow samples were collected from 20 healthy volunteers. The mitochondrial genome was amplified by polymerase chain reaction (PCR), and PCR products were used for sequencing and analysis.


RESULTS
Higher mutational rates were observed in the ND1-2, ND4-6, and CYTB genes in CAA patients suffering from Kidney yin deficiency. Moreover, the ID4 gene was unmethylated in bone marrow samples from healthy individuals, but was methylated in some CAA patients suffering from Kidney yin deficiency (positive rate, 60%) and Kidney yang deficiency (positive rate, 55%).


CONCLUSIONS
These data supported that gene mutations can alter the expression of respiratory chain enzyme complexes in CAA patients, resulting in energy metabolism impairment and promoting the physiological and pathological processes of hematopoietic failure. Functional impairment of the mitochondrial respiration chain induced by gene mutation may be an important reason for hematopoietic failure in patients with CAA. This change is closely related to maternal inheritance and Kidney yin deficiency. Finally, these data supported the assertion that it is easy to treat disease in patients suffering from yang deficiency and difficult to treat disease in patients suffering from yin deficiency.
Department of Hematology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, 250011, China. cdz45@163.com.|Postdoctoral Research Station, Shandong University, Jinan, 250012, China. cdz45@163.com.
Xing
X
Cui
Department of Hematology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, 250011, China.
Jing-Yi
JY
Wang
Department of Hematology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, 250011, China.
Kui
K
Liu
Department of Hematology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, 250011, China.
Si-Yuan
SY
Cui
Department of Hematology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, 250011, China.
Jie
J
Zhang
Department of Hematology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, 250011, China.
Ya-Qin
YQ
Luo
Department of Hematology, Shangdong Province Hospital of Shandong University, Jinan, 250021, China.
Xin
X
Wang
D000328:Adult; D000741:Anemia, Aplastic / Q000235:genetics*; D001483:Base Sequence; D001706:Biopsy; D001853:Bone Marrow / Q000473:pathology; D016022:Case-Control Studies; D002648:Child; D002908:Chronic Disease; D019175:DNA Methylation / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005260:Female; D054629:Genome, Mitochondrial / Q000235:genetics*; D006801:Humans; D051796:Inhibitor of Differentiation Proteins / Q000235:genetics*; D007668:Kidney / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009154:Mutation / Q000235:genetics*; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic / Q000235:genetics*; D016710:Yin Deficiency / Q000235:genetics*; D055815:Young Adult
D004272:DNA, Mitochondrial; C490128:ID4 protein, human; D051796:Inhibitor of Differentiation Proteins
ID4; Kidney; bone marrow; chronic aplastic anemia; maternal inheritance; methylation; mitochondrial DNA