Biophysical probing of the binding properties of a Cu(II) complex with G-quadruplex DNA: an experimental and computational study.
Telomerase inhibition through G-quadruplex stabilization by small molecules is of great interest as a novel anticancer therapeutic strategy. Here, we show that newly synthesized Cu-complex binds to G-quadruplex DNA and induces changes in its stability. This biophysical interaction was investigated in vitro using spectroscopic, voltammetric and computational techniques. The binding constant for this complex to G-quadruplex using spectroscopic and electrochemical methods is in the order of 10(5) . The binding stoichiometry was investigated using spectroscopic techniques and corresponded to a ratio of 1: 1. Fluorescence titration results reveal that Cu-complex is quenched in the presence of G-quadruplex DNA. Analysis of the fluorescence emission at different temperatures shows that ΔH° > 0, ΔS° > 0 and ΔG° < 0, and indicates that hydrophobic interactions played a major role in the binding processes. MD simulation results suggested that this ligand could stabilize the G-quadruplex structure. An optimized docked model of the G-quadruplex-ligand mixture confirmed the experimental results. Based on the results, we conclude that Cu-complex as an anticancer candidate can bind and stabilize the G-quadruplex DNA structure.
Biophysical Chemistry Laboratory, Department of Chemistry, Ferdowsi University of Mashhad, Mashhad, Iran.
Mohammad Reza
MR
Housaindokht
Biophysical Chemistry Laboratory, Department of Chemistry, Ferdowsi University of Mashhad, Mashhad, Iran.
Asma
A
Verdian-Doghaei
D001665:Binding Sites; D055592:Biophysical Phenomena; D003300:Copper / Q000737:chemistry*; D005453:Fluorescence; D054856:G-Quadruplexes; D008163:Luminescent Measurements; D056004:Molecular Dynamics Simulation; D009942:Organometallic Compounds / Q000737:chemistry*; D013816:Thermodynamics
D009942:Organometallic Compounds; D003300:Copper
G-quadruplex; UV-vis; fluorescence; molecular dynamics simulation; voltammetry