Euphorbiasteroid, a component of Euphorbia lathyris L., inhibits adipogenesis of 3T3-L1 cells via activation of AMP-activated protein kinase.
The purpose of this study is to investigate the effects of euphorbiasteroid, a component of Euphorbia lathyris L., on adipogenesis of 3T3-L1 pre-adipocytes and its underlying mechanisms. Euphorbiasteroid decreased differentiation of 3T3-L1 cells via reduction of intracellular triglyceride (TG) accumulation at concentrations of 25 and 50 μM. In addition, euphorbiasteroid altered the key regulator proteins of adipogenesis in the early stage of adipocyte differentiation by increasing the phosphorylation of AMP-activated protein kinase (AMPK) and acetyl-CoA carboxylase. Subsequently, levels of adipogenic proteins, including fatty acid synthase, peroxisome proliferator-activated receptor-γ and CCAAT/enhancer-binding protein α, were decreased by euphorbiasteroid treatment at the late stage of adipocyte differentiation. The anti-adipogenic effect of euphorbiasteroid may be derived from inhibition of early stage of adipocyte differentiation. Taken together, euphorbiasteroid inhibits adipogenesis of 3T3-L1 cells through activation of the AMPK pathway. Therefore, euphorbiasteroid and its source plant, E. lathyris L., could possibly be one of the fascinating anti-obesity agent.
Korea Food Research Institute, Division of Nutrition And Metabolism Research, Seongnam-si, Korea.
Su-Jin
SJ
Park
Korea Food Research Institute, Division of Nutrition And Metabolism Research, Seongnam-si, Korea.
Jae Ho
JH
Park
The University of Tennessee-Knoxville, Department of Nutrition, College of Education, Health & Human Sciences, Knoxville, TN, USA.
Anna
A
Han
Konkuk University, Department of Bioresources and Food Science, College of Life and Environmental Sciences, Seoul, Gwangjin-Gu, Korea.
Munkhtugs
M
Davaatseren
Gyeongsang National University, Department of Food Science & Technology, Jinju, Korea.
Hyun Jin
HJ
Kim
Korea Food Research Institute, Division of Nutrition And Metabolism Research, Seongnam-si, Korea.
Myung-Sunny
MS
Kim
Korea Food Research Institute, Division of Nutrition And Metabolism Research, Seongnam-si, Korea.
Haeng Jeon
HJ
Hur
Korea Food Research Institute, Division of Nutrition And Metabolism Research, Seongnam-si, Korea.
Mi-Jeong
MJ
Sung
Korea Food Research Institute, Division of Nutrition And Metabolism Research, Seongnam-si, Korea.
Jin-Taek
JT
Hwang
Korea Food Research Institute, Division of Nutrition And Metabolism Research, Seongnam-si, Korea.
Hye Jeong
HJ
Yang
Korea Food Research Institute, Division of Nutrition And Metabolism Research, Seongnam-si, Korea.
Dae Young
DY
Kwon
D041721:3T3-L1 Cells; D055372:AMP-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*; D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects; D050156:Adipogenesis / Q000187:drug effects* / Q000502:physiology; D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation / Q000187:drug effects; D004224:Diterpenes / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D028481:Euphorbia / Q000737:chemistry*; D050356:Lipid Metabolism / Q000187:drug effects; D051379:Mice; D010648:Phenylacetates / Q000494:pharmacology*; D010766:Phosphorylation / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects
D004224:Diterpenes; D010648:Phenylacetates; D010936:Plant Extracts; D012333:RNA, Messenger; C551893:euphorbiasteroid; D055372:AMP-Activated Protein Kinases
3T3-L1; AMPK; adipogenesis; euphorbiasteroid; obesity