Unique clonal relationship between T-cell acute lymphoblastic leukemia and subsequent Langerhans cell histiocytosis with TCR rearrangement and NOTCH1 mutation.
Acute lymphoblastic leukemia (ALL) occasionally develops before or after the onset of Langerhans cell histiocytosis (LCH). The mechanism of LCH developing after ALL remains unclear; thus the clonality of LCH developing during maintenance chemotherapy for T-cell ALL (T-ALL) was investigated. The T-ALL and LCH cells tested had the same T-cell receptor (TCR) gamma rearrangement. Mutation analysis of the NOTCH1 gene revealed 7213C>T (Q2405X) in exon 34 in T-ALL and LCH cells, but 5156T>C (I1719T) in exon 27 only in T-ALL. Polymerase chain reaction-restriction fragment length polymorphism analysis revealed three patterns of NOTCH1 mutations in T-ALL cells. The results suggest that the T-ALL and LCH cells were derived from a common precursor with TCR rearrangement and a single NOTCH1 mutation, rather than LCH cells developing from a minor clone of T-ALL with single NOTCH1 mutation.
Department of Molecular Diagnostics and Therapeutics, Kyoto Prefectural University of Medicine Graduate School of Medical Science, Kyoto, Japan.|Department of Pediatrics, The Jikei University School of Medicine, Tokyo, Japan.
Yuichi
Y
Yokokawa
Department of Molecular Diagnostics and Therapeutics, Kyoto Prefectural University of Medicine Graduate School of Medical Science, Kyoto, Japan.
Tomohiko
T
Taki
Department of Molecular Hematology and Oncology, Kyoto Prefectural University of Medicine Graduate School of Medical Science, Kyoto, Japan.
Yoshiaki
Y
Chinen
Department of Molecular Hematology and Oncology, Kyoto Prefectural University of Medicine Graduate School of Medical Science, Kyoto, Japan.
Satoru
S
Kobayashi
Department of Molecular Hematology and Oncology, Kyoto Prefectural University of Medicine Graduate School of Medical Science, Kyoto, Japan.
Hisao
H
Nagoshi
Department of Pediatrics, The Jikei University School of Medicine, Tokyo, Japan.|Division of Molecular Genetics, Institute of DNA Medicine, The Jikei University School of Medicine, Tokyo, Japan.
Masaharu
M
Akiyama
Department of Pediatrics, Jichi Medical University School of Medicine, Shimotsuke, Tochigi, Japan.
Akira
A
Morimoto
Department of Pediatrics, The Jikei University School of Medicine, Tokyo, Japan.
Hiroyuki
H
Ida
Department of Molecular Hematology and Oncology, Kyoto Prefectural University of Medicine Graduate School of Medical Science, Kyoto, Japan.
Masafumi
M
Taniwaki
D000970:Antineoplastic Agents / Q000009:adverse effects; D002648:Child; D002999:Clone Cells / Q000473:pathology; D006646:Histiocytosis, Langerhans-Cell / Q000188:drug therapy / Q000235:genetics*; D006801:Humans; D007801:Langerhans Cells / Q000473:pathology; D007964:Leukocytosis / Q000235:genetics; D008297:Male; D009154:Mutation; D054218:Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*; D051881:Receptor, Notch1 / Q000235:genetics*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics*; D013601:T-Lymphocytes / Q000473:pathology; D013921:Thrombocytopenia / Q000235:genetics
D000970:Antineoplastic Agents; C497761:NOTCH1 protein, human; D051881:Receptor, Notch1; D016692:Receptors, Antigen, T-Cell, gamma-delta