Chicken and rabbit antibodies against porcine pepsinogen A.
Isolated porcine pepsinogen A was used for the preparation of polyclonal rabbit and polyclonal chicken anti-pepsinogen A antibodies. Immunochemical properties of both immunoglobulin fractions were compared. The rabbit anti-serum was further purified using immobilized porcine pepsinogen A on magnetic cellulose beads and the resulting anti-pepsinogen A fraction proved to be applicable for the separation and the determination of porcine pepsinogen A. In contrary, antibodies prepared from chicken eggs by the same way have been found not suitable for the evaluation of the pepsinogen A level. Unexpectedly, the pre-immune fraction of chicken antibodies showed reactivity against porcine pepsinogen A and the affinity separation of specific polyclonal chicken anti-pepsinogen A antibodies on immobilized porcine pepsinogen A did not result in an enrichment of anti-pepsinogen A antibodies.
Department of Analytical Chemistry, Faculty of Science, Charles University in Prague, Prague, Czech Republic.
Lucie
L
Kulhavá
Institute of Pathological Physiology, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic.
Eva
E
Miarková
Institute of Pathological Physiology, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic.
Petr
P
Přikryl
Department of Biochemistry, Faculty of Science, Charles University in Prague, Prague, Czech Republic.
Petr
P
Hodek
Department of Biochemistry, Faculty of Science, Charles University in Prague, Prague, Czech Republic.
Marie
M
Tichá
Institute of Pathological Physiology, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic. zkuce@lf1.cuni.cz.
Zdenka
Z
Kučerová
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D002417:Cattle; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay; D005753:Gastric Mucosa / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D007150:Immunohistochemistry; D020116:Pepsinogen A / Q000276:immunology* / Q000378:metabolism; D011817:Rabbits; D013274:Stomach Neoplasms / Q000276:immunology / Q000378:metabolism; D013552:Swine
D000906:Antibodies; D020116:Pepsinogen A