Separation of anthraquinone compounds from the seed of Cassia obtusifolia L. using recycling counter-current chromatography.
Recycling counter-current chromatography (CCC) together with step-gradient CCC and medium-pressure liquid chromatography (MPLC) was employed to separate nine anthraquinone compounds from Cassia obtusifolia L. in this study. The results showed that recycling CCC is a powerful tool for compounds that are difficult to separate with common elution mode. CCC was the better option for crude material while MPLC had advantage for the final tuning. The combination of recycling CCC and MPLC could simplify the method exploring process in the separation process. The structures of these compounds were identified according to their mass spectra, by (1)H-NMR and compared with standard compounds.
State Key Laboratory of Biotherapy, West China Hospital and College of Pharmacy, Sichuan University, Chengdu, Sichuan, P. R. China; State Key Laboratory Breeding Base of Systematic Research Development of Utilization of Chinese Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, P. R. China.
Jianhong
J
Yang
Haoyu
H
Ye
Huijun
H
Lai
Shucai
S
Li
Shichao
S
He
Shijie
S
Zhong
Lijuan
L
Chen
Aihua
A
Peng
D000880:Anthraquinones / Q000737:chemistry / Q000302:isolation & purification*; D002366:Cassia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003377:Countercurrent Distribution / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D010946:Plants, Medicinal / Q000737:chemistry; D012639:Seeds / Q000737:chemistry; D012997:Solvents; D021241:Spectrometry, Mass, Electrospray Ionization
D000880:Anthraquinones; D004365:Drugs, Chinese Herbal; D012997:Solvents
Anthraquinone; Cassia obtusifolia L.; Recycling counter‐current chromatography