OlsG (Sinac_1600) Is an Ornithine Lipid N-Methyltransferase from the Planctomycete Singulisphaera acidiphila.
Ornithine lipids (OLs) are phosphorus-free membrane lipids widespread in bacteria but absent from archaea and eukaryotes. In addition to the unmodified OLs, a variety of OL derivatives hydroxylated in different structural positions has been reported. Recently, methylated derivatives of OLs were described in several planctomycetes isolated from a peat bog in Northern Russia, although the gene/enzyme responsible for the N-methylation of OL remained obscure. Here we identify and characterize the OL N-methyltransferase OlsG (Sinac_1600) from the planctomycete Singulisphaera acidiphila. When OlsG is co-expressed with the OL synthase OlsF in Escherichia coli, methylated OL derivatives are formed. An in vitro characterization shows that OlsG is responsible for the 3-fold methylation of the terminal δ-nitrogen of OL. Methylation is dependent on the presence of the detergent Triton X-100 and the methyldonor S-adenosylmethionine.
From the Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Cuernavaca, Morelos CP62210, Mexico and.
Wendy Itzel
WI
Escobedo-Hinojosa
From the Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Cuernavaca, Morelos CP62210, Mexico and.
Miguel Ángel
MÁ
Vences-Guzmán
the Department of Earth, Atmospheric and Planetary Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts 02144.
Florence
F
Schubotz
From the Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Cuernavaca, Morelos CP62210, Mexico and.
Mario
M
Sandoval-Calderón
the Department of Earth, Atmospheric and Planetary Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts 02144.
Roger E
RE
Summons
From the Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Cuernavaca, Morelos CP62210, Mexico and.
Isabel María
IM
López-Lara
From the Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Cuernavaca, Morelos CP62210, Mexico and.
Otto
O
Geiger
From the Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Cuernavaca, Morelos CP62210, Mexico and chsohlen@ccg.unam.mx.
Christian
http://orcid.org/0000-0002-9962-2859
C
Sohlenkamp
D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D017931:DNA Primers; D004926:Escherichia coli / Q000235:genetics; D008055:Lipids; D013058:Mass Spectrometry; D008563:Membrane Lipids / Q000378:metabolism; D008780:Methyltransferases / Q000378:metabolism*; D009952:Ornithine / Q000031:analogs & derivatives* / Q000378:metabolism; D010802:Phylogeny; D061328:Planctomycetales / Q000201:enzymology*
D017931:DNA Primers; D008055:Lipids; D008563:Membrane Lipids; C051524:ornithine containing aminolipid; D009952:Ornithine; D008780:Methyltransferases
lipid methylation; membrane lipid; membrane remodeling; phosphatidylcholine