Effect of l-lysine-assisted surface grafting for nano-hydroxyapatite on mechanical properties and in vitro bioactivity of poly(lactic acid-co-glycolic acid).
It is promising and challenging to study surface modification for nano-hydroxyapatite to improve the dispersion and enhance the mechanical properties and bioactivity of poly(lactic acid-co-glycolic acid). In this paper, we designed an effective new surface grafting with the assist of l-lysine for nano-hydroxyapatite, and the nano-hydroxyapatite surface grafted with the assist of l-lysine (g-nano-hydroxyapatite) was incorporated into poly(lactic acid-co-glycolic acid) to develop a series of g-nano-hydroxyapatite/poly(lactic acid-co-glycolic acid) nano-composites. The surface modification reaction for nano-hydroxyapatite, the mechanical properties, and in vitro human osteoblast-like cell (MG-63) response were characterized and investigated by Fourier transformation infrared, thermal gravimetric analysis, dispersion test, electromechanical universal tester, differential scanning calorimeter measurements, and in vitro cells culture experiment. The results showed that the grafting amount on the surface of nano-hydroxyapatite was enhanced with the increase of l-lysine, and the dispersion of nano-hydroxyapatite was improved more, so that it brought about better promotion crystallization and more excellent mechanical enhancement effect for poly(lactic acid-co-glycolic acid), comparing with the unmodified nano-hydroxyapatite. Moreover, the cells' attachment and proliferation results confirmed that the incorporation of the g-nano-hydroxyapatite into poly(lactic acid-co-glycolic acid) exhibited better biocompatibility than poly(lactic acid-co-glycolic acid). The above results indicated that the new surface grafting with the assist of l-lysine for nano-hydroxyapatite was an ideal novel surface modification method, which brought about better mechanical enhancement effect and in vitro bioactivity for poly(lactic acid-co-glycolic acid) with adding higher g-nano-hydroxyapatite content, suggesting it had a great potential to be used as bone fracture internal fixation materials in future.
Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu, PR China jlytxg@163.com.
Jiang
J
Liuyun
Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu, PR China Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, PR China.
Jiang
J
Lixin
Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu, PR China.
Xiong
X
Chengdong
Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu, PR China.
Xu
X
Lijuan
Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu, PR China.
Li
L
Ye
D018786:Bone Substitutes / Q000138:chemical synthesis*; D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line; D049109:Cell Proliferation / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis; D019245:Compressive Strength; D017886:Durapatite / Q000737:chemistry*; D055119:Elastic Modulus; D006244:Hardness; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D008239:Lysine / Q000737:chemistry*; D008422:Materials Testing; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology*; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013314:Stress, Mechanical; D013499:Surface Properties; D013718:Tensile Strength; D013814:Thermal Conductivity
D018786:Bone Substitutes; D020099:Coated Materials, Biocompatible; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D017886:Durapatite; D008239:Lysine
Nano-hydroxyapatite; bioactivity; mechanical properties; poly(lactic acid-co-glycolic acid); surface modification