Rapid determination of 4-aminobutyric acid and L-glutamic acid in biological decarboxylation process by capillary electrophoresis-mass spectrometry.
4-Aminobutylic acid (GABA) is a monomer of plastic polyamide 4. Bio-based polyamide 4 can be produced by using GABA obtained from biomass. The production of L-glutamic acid (Glu) from biomass has been established. GABA is produced by decarboxylation of Glu in biological process. High-performance liquid chromatography (HPLC) with derivatization is generally used to determine the concentration of GABA and Glu in reacted solution samples for the efficient production of GABA. In this study, we have investigated the rapid determination of GABA and Glu by capillary electrophoresis-mass spectrometry (CE-MS) without derivatization. The determination was achieved with the use of a shortened capillary, a new internal standard for GABA, and optimization of sheath liquid composition. Determined concentrations of GABA and Glu by CE-MS were compared with those by pre-column derivatization HPLC with phenylisothiocyanate. The determined values by CE-MS were close to those by HPLC with pre-column derivatization. These results suggest that the determination of GABA and Glu in reacted solution is rapid and simplified by the use of CE-MS.
National Institute of Advanced Industrial Science and Technology (AIST), Bio-based Polymers Research Group, Research Institute for Ubiquitous Energy Devices, Osaka, Japan. takeda-s@aist.go.jp
Sahori
S
Takeda
Naoko
N
Yamano
Norioki
N
Kawasaki
Hisanori
H
Ando
Atsuyoshi
A
Nakayama
D018533:Biomass; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003653:Decarboxylation; D019075:Electrophoresis, Capillary / Q000379:methods*; D018698:Glutamic Acid / Q000032:analysis*; D012015:Reference Standards; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D005680:gamma-Aminobutyric Acid / Q000032:analysis* / Q000592:standards
D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid
4‐Aminobutylic acid; Biological process; CE‐MS; L‐Glutamic acid; Polyamide 4