Population Growth and Allergen Content of Cultured Euroglyphus maynei House Dust Mites.
BACKGROUND
The house dust mite, Euroglyphus maynei, occurs in homes worldwide and is an important source of many allergens. Many patients sensitive to Dermatophagoides farinae and D. pteronyssinus are also sensitive to E. maynei. Extracts to detect sensitivity to E. maynei and reagents to detect E. maynei allergens in the environment or in cultures are not readily available. Information for the culture of E. maynei and for the determination of allergen and endotoxin levels in cultures is limited.


METHODS
We mass cultured E. maynei at 23 and 30°C and determined the population growth profiles from inoculation until cultures could be harvested for the production of extracts. We also developed an ELISA to measure Eur m 1 and Eur m 2 allergens using mouse monoclonal antibodies directed at cross-reacting epitopes of group 1 and group 2 allergens of D. farinae and D. pteronyssinus.


RESULTS
The E. maynei populations grew exponentially at both 23 and 30°C; however, the cultures matured more rapidly at 23°C. The Eur m 1 and Eur m 2 allergen concentrations in culture extracts changed independently as the cultures grew and matured. At both temperatures, the Eur m 1 concentrations increased as the cultures matured, while the Eur m 2 concentrations did not. The endotoxin levels in these cultures were low.


CONCLUSIONS
We report here that E. maynei can be cultured at 23 and 30°C. Monoclonal antibodies directed at cross-reacting epitopes on Dermatophagoides allergens can be used to measure the associated E. maynei allergen levels in these cultures.
Department of Biological Sciences, Wright State University, Dayton, Ohio, USA.
Marjorie S
MS
Morgan
DiAnn L
DL
Vyszenski-Moher
Larry G
LG
Arlian
D000818:Animals; D039741:Antigens, Dermatophagoides / Q000032:analysis*; D018929:Cell Culture Techniques / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D011158:Population Growth; D039981:Pyroglyphidae / Q000254:growth & development* / Q000276:immunology*
D039741:Antigens, Dermatophagoides