Why solid-state fermentation is more advantageous over submerged fermentation for converting high concentration of glycerol into Monacolin K by Monascus purpureus 9901: A mechanistic study.
The underlying mechanisms by which solid-state fermentation (SSF) was more advantageous over submerged fermentation (SmF) for converting high concentration of glycerol into Monacolin K by Monascus purpureus were investigated innovatively. First, the established kinetic models and kinetic parameters showed that the cell growth, Monacolin K formation and glycerol consumption in SSF were more rapid than those in SmF. Secondly, the comparison of fatty acid composition of mycelial cells indicated a better fluidity and permeability of the cell membrane in SSF than that of SmF, which was also consistent with the difference in the ratio of extracellular/intracellular Monacolin K between the two systems. Thirdly, the phenomenon of glycerol concentration gradient was verified in SSF, which could well explain the resistance effect to high concentration of glycerol in SSF. These new findings provide some important insights to the elucidation of the advantages of SSF for the synthesis of fungal secondary metabolites.
Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China. Electronic address: superzbobo@gmail.com.
Bo-Bo
BB
Zhang
Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
Li-Ping
LP
Lu
Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China. Electronic address: grxu527@126.com.
Gan-Rong
GR
Xu
D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002463:Cell Membrane Permeability; D005227:Fatty Acids / Q000378:metabolism; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism*; D007700:Kinetics; D008148:Lovastatin / Q000378:metabolism*; D032741:Monascus / Q000378:metabolism*
D005227:Fatty Acids; D008148:Lovastatin; D005990:Glycerol
Glycerol; Monacolin K; Monascus purpureus; Solid-state fermentation; Submerged fermentation