Correlation between B7-H3 expression and rheumatoid arthritis: A new polymorphism haplotype is associated with increased disease risk.
CD276 (B7-H3) is a costimulatory molecule that plays a potent role in T cell responses, however, the role of B7-H3 in autoimmune diseases has not been elucidated. We analyzed B7-H3 expression in rheumatoid arthritis (RA) for the first time and found B7-H3 was significantly up-regulated on monocytes in RA patients, while the levels of soluble B7-H3 in serum were lower than in controls (P < 0.0001). These differences correlated with clinical and laboratory disease parameters and informatory factor TNF-α. Through in vitro experiments, we demonstrated that B7-H3 promoted TNF-α secretion. In addition, a new polymorphism variant, B7-H3-T-A-C-T, was identified and shown to be associated with the incidence of RA and the decreased release of sB7-H3. These results suggest that B7-H3 may be a promising biomarker associated with the pathogenesis of RA. Notably, the new B7-H3-T-A-C-T polymorphism variant is associated with RA risk and might be associated with the release of soluble B7-H3.
Institute of Medical Biotechnology, Suzhou Health College, Suzhou, Jiangsu 215009, PR China. Electronic address: jsun@szhct.edu.cn.
Jing
J
Sun
Jiangsu Institute of Clinical Immunology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215007, PR China.
Cuiping
C
Liu
Wuxi Red Cross Blood Center, Wuxi, PR China.
Li
L
Gao
Institute of Medical Biotechnology, Suzhou Health College, Suzhou, Jiangsu 215009, PR China.
Yundi
Y
Guo
Institute of Medical Biotechnology, Suzhou Health College, Suzhou, Jiangsu 215009, PR China.
Yaqin
Y
Zhang
Institute of Medical Biotechnology, Suzhou Health College, Suzhou, Jiangsu 215009, PR China.
Pingping
P
Wu
Department of Special Requirements Ward, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, PR China.
Juean
J
Jiang
Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, PR China.
Ruhong
R
Yan
Jiangsu Institute of Clinical Immunology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215007, PR China. Electronic address: xueguangzh@126.com.
Xueguang
X
Zhang
D001172:Arthritis, Rheumatoid / Q000235:genetics* / Q000378:metabolism; D060887:B7 Antigens / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D016022:Case-Control Studies; D002460:Cell Line; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D009000:Monocytes / Q000378:metabolism*; D020641:Polymorphism, Single Nucleotide; D012333:RNA, Messenger / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D012720:Severity of Illness Index; D014409:Tumor Necrosis Factor-alpha / Q000187:drug effects / Q000378:metabolism*; D015854:Up-Regulation
D060887:B7 Antigens; C423237:CD276 protein, human; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha
B7-H3/CD276; Gene expression; Rheumatoid arthritis (RA); Single nucleotide polymorphisms (SNPs)