[Screening of specific proteins in ultraviolet-induced acute damage of human fibroblasts using proteinchip with surface enhanced laser desorption/ ionization time-of-flight mass spectrometry].
OBJECTIVE
To investigate specific proteins in ultraviolet (UV)-induced acute damage of human fibroblasts using proteinchip with surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS).


METHODS
CM10 Proteinchip and SELDI-TOF-MS were used for detecting protein samples of non-irradiated group, UVA (2.5 J/cm(2))-irradiated group and UVB (100 mJ/cm(2))-irradiated group of normal human fibroblasts.


RESULTS
Ten protein peaks were down-regulated and 8 protein peaks up-regulated in fibroblasts after UV irradiation. By searching in SWISS-PROT and TrEMBLE databases, the peaks of 11 320.1 and 8 574.3 accorded with those of caspase-7 precursor and cytochrome C oxidase polypeptide VIc precursor proteins.


CONCLUSIONS
As a quick and convenient high-throughout proteomic analytic method, SELDI-TOF-MS plus proteinchip offers a unique platform for detecting dermal photodamage.
Department of Dermatology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
Yan
Y
Yan
Haoxiang
H
Xu
Yanhua
Y
Zheng
Jie
J
Liu
Jianzhong
J
Zhang
Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College,Nanjing 210042, China. Email: wangbx@ncstdlc.org.
Baoxi
B
Wang
D005347:Fibroblasts; D006801:Humans; D040081:Protein Array Analysis; D011506:Proteins / Q000032:analysis*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014466:Ultraviolet Rays
D011506:Proteins