Comparative studies on the interactions of baicalein and Al(III)-baicalein complex with human serum albumin.
A new potential drug aluminum(III)-baicalein complex (ALBC) was synthesized and characterized. The binding mechanisms of baicalein (BC) and ALBC to human serum albumin (HSA) under simulative physiological conditions were investigated, in order to understand the pharmacokinetics of BC and ALBC. Fluorescence spectroscopy results suggested that the binding level of BC is higher than that of ALBC. Results of UV-vis, synchronous fluorescence, 3D fluorescence, circular dichroism and Fourier transform infrared spectroscopic analyses consistently demonstrated that the conformation of HSA was altered when bound to BC or ALBC. The distance between HSA as a donor and BC (or ALBC) as an acceptor was determined via fluorescence resonance energy transfer. The results of competitive experiments and molecular docking studies indicated that BC was located in site I (subdomain IIA) on HSA and that ALBC was bound to HSA mainly within site II (subdomain IIIA).
College of Chemical Engineering, Sichuan University, Chengdu, 610065, People's Republic of China.
Jing
J
Wang
College of Chemical Engineering, Sichuan University, Chengdu, 610065, People's Republic of China.
Qing
Q
Wang
College of Chemical Engineering, Sichuan University, Chengdu, 610065, People's Republic of China.
Di
D
Wu
College of Chemical Engineering, Sichuan University, Chengdu, 610065, People's Republic of China.
Jin
J
Yan
College of Chemical Engineering, Sichuan University, Chengdu, 610065, People's Republic of China.
You
Y
Wu
College of Chemical Engineering, Sichuan University, Chengdu, 610065, People's Republic of China.
Hui
H
Li
D000535:Aluminum / Q000737:chemistry*; D044950:Flavanones / Q000737:chemistry*; D006801:Humans; D062105:Molecular Docking Simulation; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics
D044950:Flavanones; D009942:Organometallic Compounds; D012709:Serum Albumin; C006680:baicalein; D000535:Aluminum
aluminum(III)-baicalein complex; baicalein; fluorescence quenching; human serum albumin; molecular docking