Whole slurry saccharification and fermentation of maleic acid-pretreated rice straw for ethanol production.
We evaluated the feasibility of whole slurry (pretreated lignocellulose) saccharification and fermentation for producing ethanol from maleic acid-pretreated rice straw. The optimized conditions for pretreatment were to treat rice straw at a high temperature (190 °C) with 1 % (w/v) maleic acid for a short duration (3 min ramping to 190 °C and 3 min holding at 190 °C). Enzymatic digestibility (based on theoretical glucose yield) of cellulose in the pretreated rice straw was 91.5 %. Whole slurry saccharification and fermentation of pretreated rice straw resulted in 83.2 % final yield of ethanol based on the initial quantity of glucan in untreated rice straw. These findings indicate that maleic acid pretreatment results in a high yield of ethanol from fermentation of whole slurry even without conditioning or detoxification of the slurry. Additionally, the separation of solids and liquid is not required; therefore, the economics of cellulosic ethanol fuel production are significantly improved. We also demonstrated whole slurry saccharification and fermentation of pretreated lignocellulose, which has rarely been reported.
Department of Biotechnology, Korea University Graduate School, Seoul, 136-713, Republic of Korea.
Young Hoon
YH
Jung
Hyun Min
HM
Park
Kyoung Heon
KH
Kim
D050260:Carbohydrate Metabolism / Q000502:physiology; D000431:Ethanol / Q000302:isolation & purification / Q000378:metabolism*; D005240:Feasibility Studies; D005285:Fermentation; D008031:Lignin / Q000737:chemistry* / Q000378:metabolism*; D008298:Maleates / Q000737:chemistry*; D012275:Oryza / Q000737:chemistry* / Q000382:microbiology*; D018547:Plant Stems / Q000737:chemistry / Q000382:microbiology
D008298:Maleates; C036909:lignocellulose; D000431:Ethanol; D008031:Lignin; C030272:maleic acid