ATR-FTIR measurements of albumin and fibrinogen adsorption: Inert versus calcium phosphate ceramics.
Arthritis, bone fracture, bone tumors and other musculoskeletal diseases affect millions of people across the world. Nowadays, inert and bioactive ceramics are used as bone substitutes or for bone regeneration. Their bioactivity is very much dictated by the way proteins adsorb on their surface. In this work, we compared the adsorption of albumin and fibrinogen on inert and calcium phosphates ceramics (CaPs) using attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) to follow in situ protein adsorption on these materials. To this effect, we developed a sol-gel technique to control the surface chemistry of an ATR-FTIR detector. Hydroxyapatite adsorbed more albumin and β-tricalcium phosphate adsorbed more fibrinogen. Biphasic calcium phosphate presented the lowest adsorption among CaP for both proteins, illustrating the effect of surface heterogeneities. Inert ceramics adsorbed a lower amount of both proteins compared with bioactive ceramics. A significant change was observed in the conformation of the adsorbed protein versus the surface chemistry. Hydroxyapatite produced a larger loss of α-helix structure on albumin and biphasic calcium phosphate reduced β-sheet percentage on fibrinogen. Inert ceramics produced large α-helix loss on albumin and presented weak interaction with fibrinogen. Zirconia did not adsorb albumin and titanium dioxide promoted huge denaturalization of fibrinogen.
Université De Mons-UMONS, Laboratoire De Physique Des Surfaces Et Des Interfaces, Avenue Maistriau 19, Mons, 7000, Belgium.
Marcel
M
Boix
Department of Chemical Engineering, University of Bath, United Kingdom.|Department of Materials, Imperial College London, Center for Advanced Structural Ceramics, United Kingdom.
Salvador
S
Eslava
Department of Materials, Imperial College London, Center for Advanced Structural Ceramics, United Kingdom.
Gil
G
Costa Machado
Université De Mons-UMONS, Laboratoire De Physique Des Surfaces Et Des Interfaces, Avenue Maistriau 19, Mons, 7000, Belgium.
Emmanuel
E
Gosselin
Department of Materials, Imperial College London, Center for Advanced Structural Ceramics, United Kingdom.
Na
N
Ni
Department of Materials, Imperial College London, Center for Advanced Structural Ceramics, United Kingdom.
Eduardo
E
Saiz
Université De Mons-UMONS, Laboratoire De Physique Des Surfaces Et Des Interfaces, Avenue Maistriau 19, Mons, 7000, Belgium.
Joël
J
De Coninck
D000327:Adsorption; D000418:Albumins / Q000378:metabolism*; D000577:Amides / Q000737:chemistry; D000818:Animals; D002130:Calcium Phosphates / Q000494:pharmacology*; D002417:Cattle; D002516:Ceramics / Q000494:pharmacology*; D017886:Durapatite / Q000494:pharmacology; D005340:Fibrinogen / Q000378:metabolism*; D006801:Humans; D017550:Spectroscopy, Fourier Transform Infrared; D014025:Titanium / Q000494:pharmacology; D014961:X-Ray Diffraction; D015040:Zirconium / Q000494:pharmacology
D000418:Albumins; D000577:Amides; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; C009495:titanium dioxide; D005340:Fibrinogen; D017886:Durapatite; C020243:calcium phosphate; D015040:Zirconium; D014025:Titanium; C028541:zirconium oxide
FTIR and ATR; calcium phosphates; protein adsorption; sol-gel