Celery Seed Extract Blocks Peroxide Injury in Macrophages via Notch1/NF-κB Pathway.
Oxidized low-density lipoprotein (ox-LDL)-induced macrophage foam cell formation and injury is one of the major atherogenic factors. This study is aimed to investigate the protective effect of celery seed extract (CSE) on ox-LDL-induced injury of macrophages and the underlying signaling pathway. RAW264.7 macrophages were pre-incubated with CSE for 24 h, followed by stimulation with ox-LDL. Oil red O staining and enzymatic colorimetry indicated CSE significantly lessened lipid droplets and total cholesterol (TC) content in ox-LDL-injured macrophages. ELISA revealed that CSE decreased the secretion of inflammatory cytokine TNF-α and IL-6 by 12-27% and 5-15% respectively. MTT assay showed CSE promoted cell viability by 16-40%. Cell apoptosis was also analyzed by flow cytometry and laser scanning confocal microscope and the data indicated CSE inhibited ox-LDL-induced apoptosis of macrophages. Meanwhile, western blot analysis showed CSE suppressed NF-κBp65 and notch1 protein expressions stimulated by ox-LDL in macrophages. These results suggest that CSE inhibits ox-LDL-induced macrophages injury via notch1/NF-κB pathway.
College of Basic Medical Sciences, Taishan Medical University, Shandong, China.
Yanhong
Y
Si
Shoudong
S
Guo
Yongqi
Y
Fang
Shucun
S
Qin
Furong
F
Li
Ying
Y
Zhang
Peng
P
Jiao
Chunduo
C
Zhang
Linlin
L
Gao
D000818:Animals; D000077102:Apium; D017209:Apoptosis / Q000187:drug effects*; D050197:Atherosclerosis / Q000209:etiology; D015536:Down-Regulation / Q000187:drug effects; D018836:Inflammation Mediators / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000009:adverse effects*; D008264:Macrophages / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology*; D000067996:RAW 264.7 Cells; D051881:Receptor, Notch1 / Q000378:metabolism*; D012639:Seeds; D015398:Signal Transduction / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D018836:Inflammation Mediators; D015850:Interleukin-6; D008077:Lipoproteins, LDL; D016328:NF-kappa B; D010936:Plant Extracts; D051881:Receptor, Notch1; D014409:Tumor Necrosis Factor-alpha; C099252:oxidized low density lipoprotein
Apium graveolens; Apoptosis; Celery Seed Extract; Inflammation; Macrophage; Oxidized Low-Density Lipoprotein