A comprehensive study of the harmful effects of ZnO nanoparticles using Drosophila melanogaster as an in vivo model.
This study planned to determine the range of biological effects associated with ZnO-NP exposure using Drosophila melanogaster as an in vivo model. In addition, ZnCl2 was used to determine the potential role of Zn ions alone. Toxicity, internalization through the intestinal barrier, gene expression changes, ROS production, and genotoxicity were the end-points evaluated. No toxicity or oxidative stress induction was observed in D. melanogaster larvae, whether using ZnO-NPs or ZnCl2. Internalization of ZnO-NPs through the intestinal barrier was observed. No significant changes in the frequency of mutant clones (wing-spot test) or percentage of DNA in tail (comet assay) were observed although significant changes in Hsp70 and p53 gene expression were detected. Our study shows that ZnO-NPs do not induce toxicity or genotoxicity in D. melanogaster, although uptake occurs and altered gene expression is observed.
Grup de Mutagènesi, Departament de Genètica i de Microbiologia, Facultat de Biociències, Universitat Autònoma de Barcelona, Campus de Bellaterra, 08193 Cerdanyola del Vallès, Barcelona, Spain; Sohag University, Faculty of Sciences, Zoology Department, 82524-Campus, Sohag, Egypt.
Mohamed
M
Alaraby
Grup de Mutagènesi, Departament de Genètica i de Microbiologia, Facultat de Biociències, Universitat Autònoma de Barcelona, Campus de Bellaterra, 08193 Cerdanyola del Vallès, Barcelona, Spain.
Balasubramanyam
B
Annangi
Grup de Mutagènesi, Departament de Genètica i de Microbiologia, Facultat de Biociències, Universitat Autònoma de Barcelona, Campus de Bellaterra, 08193 Cerdanyola del Vallès, Barcelona, Spain; CIBER Epidemiología y Salud Pública, ISCIII, Madrid, Spain.
Alba
A
Hernández
Grup de Mutagènesi, Departament de Genètica i de Microbiologia, Facultat de Biociències, Universitat Autònoma de Barcelona, Campus de Bellaterra, 08193 Cerdanyola del Vallès, Barcelona, Spain; CIBER Epidemiología y Salud Pública, ISCIII, Madrid, Spain.
Amadeu
A
Creus
Grup de Mutagènesi, Departament de Genètica i de Microbiologia, Facultat de Biociències, Universitat Autònoma de Barcelona, Campus de Bellaterra, 08193 Cerdanyola del Vallès, Barcelona, Spain; CIBER Epidemiología y Salud Pública, ISCIII, Madrid, Spain. Electronic address: ricard.marcos@uab.es.
Ricard
R
Marcos
D000818:Animals; D020552:Comet Assay; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D004784:Environmental Monitoring / Q000379:methods*; D015870:Gene Expression / Q000187:drug effects; D006434:Hemocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D007814:Larva / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D008954:Models, Biological; D053758:Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects; D010316:Particle Size; D017382:Reactive Oxygen Species / Q000378:metabolism; D013499:Surface Properties; D015034:Zinc Oxide / Q000737:chemistry / Q000633:toxicity*
D017382:Reactive Oxygen Species; D015034:Zinc Oxide
Comet assay; Drosophila melanogaster; Genotoxicity; Wing-spot assay; ZnO nanoparticles