Deregulated Levels of the NF-κB1, NF-κB2, and Rel Genes in Ukrainian Patients with Leukemia and Lymphoma in the Post-Chernobyl Period.
OBJECTIVE
Nuclear factor kappa B (NF-κB) is an important transcription factor in cancer and NF-κB activation has been seen in angiogenesis, tumor progression, and metastasis. Relationships between specific NF-κB gene networks, leukemogenesis, and radiation exposure are still unknown. Our aim was to study the expression levels of the NF-κB1, NF-κB2, and Rel genes in hematological malignancies in the post-Chernobyl period.


METHODS
We analyzed gene expression levels of NF-κB1, NF-κB2, and Rel in 49 B-cell chronic lymphocytic leukemia, 8 B-cell non-Hodgkin's lymphoma, 3 acute myeloid leukemia, 3 chronic myeloid leukemia, 2 hairy cell leukemia, 2 myelodysplastic syndrome, and 2 T-cell large granular lymphocytic leukemia patients using real-time polymerase chain reaction.


RESULTS
Expression levels of NF-κB1, NF-κB2, and Rel genes were found to be deregulated.


CONCLUSIONS
These results could be accepted as specific gene traces to radiation-induced leukemia or as potential candidates for new diagnostic biomarker studies. Larger experiments and non-exposed control malignant cell populations are needed to clarify these suggestions.
Hakan
H
Savlı
Ramis Ufuk
RU
Akkoyunlu
Naci
N
Çine
Daniil F
DF
Gluzman
Michael P
MP
Zavelevich
Lilia M
LM
Sklyarenko
Stella V
SV
Koval
Kocaeli University Faculty of Medicine, Department of Medical Genetics, Kocaeli, Turkey. E-mail : sun_deniz@hotmail.com.
Deniz
D
Sünnetçi
D000328:Adult; D000368:Aged; D047852:Chernobyl Nuclear Accident; D005260:Female; D020573:Genes, rel; D006801:Humans; D007953:Leukemia, Radiation-Induced / Q000453:epidemiology / Q000209:etiology / Q000235:genetics*; D008223:Lymphoma / Q000453:epidemiology / Q000209:etiology / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000453:epidemiology / Q000209:etiology / Q000235:genetics / Q000378:metabolism; D016328:NF-kappa B / Q000096:biosynthesis / Q000235:genetics*; D052002:NF-kappa B p50 Subunit / Q000096:biosynthesis / Q000235:genetics*; D052003:NF-kappa B p52 Subunit / Q000096:biosynthesis / Q000235:genetics*; D009381:Neoplasms, Radiation-Induced / Q000453:epidemiology / Q000209:etiology / Q000235:genetics* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D051996:Transcription Factor RelA / Q000096:biosynthesis / Q000235:genetics*; D014455:Ukraine / Q000453:epidemiology; D055815:Young Adult
D016328:NF-kappa B; D052002:NF-kappa B p50 Subunit; D052003:NF-kappa B p52 Subunit; D051996:Transcription Factor RelA