Injectable Hyaluronan Hydrogels with Peptide-Binding Dendrimers Modulate the Controlled Release of BMP-2 and TGF-β1.
BMP-2 and TGF-β1 released from injectable thermoresponsive hydrogels are studied in the presence and absence of branched macromolecules bearing BMP-2 or TGF-β1 affinity binding peptides. The synthesized branched macromolecules and the gelling compositions before and after loading with either BMP-2 or TGF-β1 are characterized physico-chemically and show a significantly lower amount of proteins released in the presence of the affinity binding peptide macromolecules. This study illustrates the potential of affinity binding peptide functionalized dendrimers to modulate the local delivery and availability of growth factors important for musculoskeletal regeneration therapies.
AO Research Institute Davos, Clavadelerstrasse 8, 7270, Davos, Platz, Switzerland.|Universitat de Barcelona, Martí i Franquès 1, 08028, Barcelona, Spain.
Ryan J
RJ
Seelbach
Institute of Research in Biomedicine, Baldiri Reixac 10-12, 08028, Barcelona, Spain.|Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine, Baldiri Reixac 10-12, 08028 , Barcelona, Spain.
Peter
P
Fransen
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine, Baldiri Reixac 10-12, 08028 , Barcelona, Spain.
Daniel
D
Pulido
AO Research Institute Davos, Clavadelerstrasse 8, 7270, Davos, Platz, Switzerland.
Matteo
M
D'Este
Universitätsklinik Freiburg, Hugstetter Str. 55, D-79106, Freiburg, Germany.
Fabian
F
Duttenhoefer
Universitätsklinik Freiburg, Hugstetter Str. 55, D-79106, Freiburg, Germany.
Sebastian
S
Sauerbier
Universitätsklinikum Goethe Universität, Schleusenweg 2-16, D-60538, Frankfurt am Main, Germany.
Thomas M
TM
Freiman
Universitätsklinik Freiburg, Hugstetter Str. 55, D-79106, Freiburg, Germany.
Philipp
P
Niemeyer
Institute of Research in Biomedicine, Baldiri Reixac 10-12, 08028, Barcelona, Spain.|Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine, Baldiri Reixac 10-12, 08028 , Barcelona, Spain.
Fernando
F
Albericio
AO Research Institute Davos, Clavadelerstrasse 8, 7270, Davos, Platz, Switzerland.
Mauro
M
Alini
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine, Baldiri Reixac 10-12, 08028 , Barcelona, Spain.|Combinatorial Chemistry Unit, Barcelona Science Park, Baldiri Reixac 10-12, 08028, Barcelona, Spain.
Miriam
M
Royo
Queen Mary, University of London, Mile End Road, E1 4NS , London, UK.
Alvaro
A
Mata
AO Research Institute Davos, Clavadelerstrasse 8, 7270, Davos, Platz, Switzerland. david.eglin@aofoundation.org.
David
D
Eglin
D000596:Amino Acids / Q000737:chemistry; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry* / Q000378:metabolism; D003692:Delayed-Action Preparations; D050091:Dendrimers / Q000737:chemistry / Q000494:pharmacology; D016503:Drug Delivery Systems; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000494:pharmacology; D020100:Hydrogels / Q000737:chemistry / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D036103:Nanotechnology; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology; D011485:Protein Binding; D012212:Rheology; D053773:Transforming Growth Factor beta1 / Q000737:chemistry* / Q000378:metabolism
D000596:Amino Acids; C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D003692:Delayed-Action Preparations; D050091:Dendrimers; D020100:Hydrogels; D010455:Peptides; D053773:Transforming Growth Factor beta1; D006820:Hyaluronic Acid
dendrimers; drug delivery; hyaluronan; injectable hydrogels; nanotechnology