Chemotypic and genetic diversity in Epimedium sagittatum from different geographical regions of China.
Different geographical plant populations within a single species can exhibit variation, in the production of secondary metabolites. Genetic and environmental variations both contribute to differences between populations; however, the relative importance of these factors is unclear. Here, the extent of variation in the production of four flavonoid glycosides (epimedin A, B, C and icariin) were investigated in eleven wild populations of Epimedium sagittatum used in traditional Chinese medicine. Secondary metabolite profiles were classified into five chemotypes. A common garden experiment indicated this chemotype variation has a significant genetic basis. Extensive genetic variation among intraspecific populations was shown using a retrotransposon-based molecular marker system. These results will assist in development of strategies for conservation, utilization and domestication of E. sagittatum.
Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei 430074, China.
Jianjun
J
Chen
College of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 330006, China.
Yanqin
Y
Xu
Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei 430074, China.
Guoyan
G
Wei
Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei 430074, China.
Sihong
S
Liao
Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei 430074, China.
Yanjun
Y
Zhang
Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei 430074, China.
Wenjun
W
Huang
Department of Plant and Soil Sciences, University of Kentucky, Lexington, KY 40546, USA.
Ling
L
Yuan
Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei 430074, China. Electronic address: yingwang@wbgcas.cn.
Ying
Y
Wang
D002681:China; D004365:Drugs, Chinese Herbal; D034181:Epimedium / Q000737:chemistry* / Q000235:genetics*; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry; D014644:Genetic Variation; D006027:Glycosides / Q000032:analysis* / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry* / Q000235:genetics*
D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D006027:Glycosides; C529106:epimedin A; C056599:icariin
Berberidaceae; Chemotypic diversity; Epimedium sagittatum; Flavonoid glycosides; Genetic diversity; Plant secondary metabolites; Population structure; iPBS markers