Enterobacter asburiae KE17 association regulates physiological changes and mitigates the toxic effects of heavy metals in soybean.
This study aimed to elucidate the role played by Enterobacter asburiae KE17 in the growth and metabolism of soybeans during copper (100 μm Cu) and zinc (100 μm Zn) toxicity. When compared to controls, plants grown under Cu and Zn stress exhibited significantly lower growth rates, but inoculation with E. asburiae KE17 increased growth rates of stressed plants. The concentrations of plant hormones (abscisic acid and salicylic acid) and rates of lipid peroxidation were higher in plants under heavy metal stress, while total chlorophyll, carotenoid content and total polyphenol concentration were lower. While the bacterial treatment reduced the abscisic acid and salicylic acid content and lipid peroxidation rate of Cu-stressed plants, it also increased the concentration of photosynthetic pigments and total polyphenol. Moreover, the heavy metals induced increased accumulation of free amino acids such as aspartic acid, threonine, serine, glycine, alanine, leucine, isoleucine, tyrosine, proline and gamma-aminobutyric acid, while E. asburiae KE17 significantly reduced concentrations of free amino acids in metal-affected plants. Co-treatment with E. asburiae KE17 regulated nutrient uptake by enhancing nitrogen content and inhibiting Cu and Zn accumulation in soybean plants. The results of this study suggest that E. asburiae KE17 mitigates the effects of Cu and Zn stress by reprogramming plant metabolic processes.
School of Applied Biosciences, Kyungpook National University, Daegu, Korea.
S-M
SM
Kang
School of Applied Biosciences, Kyungpook National University, Daegu, Korea.
R
R
Radhakrishnan
Korean Agricultural Culture Collection, Agricultural Microbiology Division, National Academy of Agricultural Science, RDA, Daegu, Korea.
Y-H
YH
You
Department of Biological Sciences and Chemistry, University of Nizwa, Nizwa, Oman.
A-L
AL
Khan
School of Ecology and Environmental Science, Kyungpook National University, Sangju, Korea.
K-E
KE
Lee
School of Applied Biosciences, Kyungpook National University, Daegu, Korea.
J-D
JD
Lee
School of Applied Biosciences, Kyungpook National University, Daegu, Korea.
I-J
IJ
Lee
D000040:Abscisic Acid / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D001483:Base Sequence; D002338:Carotenoids / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D003300:Copper / Q000633:toxicity*; D004754:Enterobacter / Q000235:genetics / Q000502:physiology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008969:Molecular Sequence Data; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000378:metabolism*; D059808:Polyphenols / Q000378:metabolism; D020156:Salicylic Acid / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013025:Glycine max / Q000187:drug effects / Q000382:microbiology* / Q000502:physiology; D013312:Stress, Physiological; D015032:Zinc / Q000633:toxicity*
D000596:Amino Acids; D010937:Plant Growth Regulators; D059808:Polyphenols; D002734:Chlorophyll; D002338:Carotenoids; D000040:Abscisic Acid; D003300:Copper; D015032:Zinc; D020156:Salicylic Acid
Amino acids; Enterobacter asburiae; metal stress; nutrients; plant hormones