Determination of deoxyribonucleoside triphosphate concentrations in yeast cells by strong anion-exchange high-performance liquid chromatography coupled with ultraviolet detection.
DNA polymerase assays are commonly used for the detection of deoxyribonucleoside triphosphates (dNTPs) in biological samples. For better specificity and accuracy, high-performance liquid chromatography (HPLC) methods have been developed for the analysis of the four dNTPs in complex samples. Here we describe a simple method using isocratic strong anion-exchange (SAX) chromatographic separation coupled with ultraviolet detection (UV) for the analysis of the four dNTPs in budding yeast Saccharomyces cerevisiae. This method can be applied to other species of yeast or bacteria.
Department of Medical Biochemistry and Biophysics, Umeå University, KBC-Building, 6th Floor, Umeå, 901 87, Sweden.
Shaodong
S
Jia
Lisette
L
Marjavaara
Robert
R
Buckland
Sushma
S
Sharma
Andrei
A
Chabes
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003854:Deoxyribonucleotides / Q000032:analysis*; D007474:Ion Exchange; D012015:Reference Standards; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D012996:Solutions; D014466:Ultraviolet Rays
D003854:Deoxyribonucleotides; D012996:Solutions