Isolation and bioactivity of diterpenoids from the roots of Salvia grandifolia.
A phytochemical investigation of root extracts of Salvia grandifolia led to isolation of six previously unreported diterpenoids, grandifolias A-F, along with eight known compounds. The structures of grandifolias A-F were primarily established by extensive 1D and 2D NMR spectroscopic analyses, as well as HRESIMS data. Their absolute configurations were assigned by their calculated and experimental electronic circular dichroism spectra or by X-ray diffraction analysis. All of the diterpenoids were evaluated for their vasorelaxant effects. Grandifolia B and isograndifoliol both exhibited dose-dependent vasorelaxant effects on rat aortic rings, preconstricted by KCl or norepinephrine, with EC50 values of 36.36-74.51μg/mL.
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 1 Xiannongtan Street, Beijing 100050, China. Electronic address: jiekang@imm.ac.cn.
Jie
J
Kang
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 1 Xiannongtan Street, Beijing 100050, China. Electronic address: annaleelin@imm.ac.cn.
Li
L
Li
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 1 Xiannongtan Street, Beijing 100050, China. Electronic address: wdd1064473619@163.com.
Dingding
D
Wang
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 1 Xiannongtan Street, Beijing 100050, China. Electronic address: wanghongqing@imm.ac.cn.
Hongqing
H
Wang
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 1 Xiannongtan Street, Beijing 100050, China. Electronic address: liuchao@imm.ac.cn.
Chao
C
Liu
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 1 Xiannongtan Street, Beijing 100050, China. Electronic address: libaoming@imm.ac.cn.
Baoming
B
Li
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 1 Xiannongtan Street, Beijing 100050, China. Electronic address: yanyu@imm.ac.cn.
Yu
Y
Yan
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 1 Xiannongtan Street, Beijing 100050, China. Electronic address: fanglh@imm.ac.cn.
Lianhua
L
Fang
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 1 Xiannongtan Street, Beijing 100050, China. Electronic address: dugh@imm.ac.cn.
Guanhua
G
Du
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 1 Xiannongtan Street, Beijing 100050, China. Electronic address: rych@imm.ac.cn.
Ruoyun
R
Chen
D045784:Abietanes / Q000737:chemistry; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry; D018360:Crystallography, X-Ray; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D008968:Molecular Conformation; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D018517:Plant Roots / Q000737:chemistry; D051381:Rats; D027544:Salvia / Q000737:chemistry*; D014665:Vasodilator Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
D045784:Abietanes; D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; D004365:Drugs, Chinese Herbal; D014665:Vasodilator Agents; C000605021:grandifolia B
Absolute configuration; Biological activity; Diterpenoid; Isolation; Labiatae; Salvia grandifolia; Structural elucidation; Vasorelaxant effect