Isolation and Analysis of Cell Wall Proteome in Elsholtzia splendens Roots Using ITRAQ with LC-ESI-MS/MS.
Cell wall proteins (CWPs) are a prime site for signal perception and defense responses to environmental stresses. To gain further insights into CWPs and their molecular function, traditional techniques (e.g., two-dimensional gel electrophoresis) may be ineffective for special proteins. Elsholtzia splendens is a copper-tolerant plant species that grow on copper deposits. In this study, a fourplex isobaric tag was used for relative and absolute quantitation with liquid chromatography-tandem mass spectrometry approach to analyze the root CWPs of E. splendens. A total of 479 unique proteins were identified, including 121 novel proteins. Approximately 80.79 % of the proteins were extracted in the CaCl2 fraction, 16.08 % were detected in the NaCl fraction, and 3.13 % were identified in both fractions. The identified proteins have been involved in various processes, including cell wall remodeling, signal transduction, defense, and carbohydrate metabolism, thereby indicating a complex regulatory network in the apoplast of E. splendens roots. This study presents the first large-scale analysis of CWPs in metal-tolerant plants, which may be of paramount importance to understand the molecular functions and metabolic pathways in the root cell wall of copper-tolerant plants.
Institute of Environmental Science and Technology, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, People's Republic of China.
Tingting
T
Liu
Canke
C
Huang
Chaofeng
C
Shen
Jiyan
J
Shi
D000222:Adaptation, Physiological; D050260:Carbohydrate Metabolism / Q000235:genetics; D002473:Cell Wall / Q000737:chemistry / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D005591:Chemical Fractionation; D002853:Chromatography, Liquid; D003300:Copper / Q000633:toxicity; D018506:Gene Expression Regulation, Plant; D019686:Lamiaceae / Q000737:chemistry / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D058977:Molecular Sequence Annotation; D057865:Plant Immunity / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification*; D018517:Plant Roots / Q000737:chemistry / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000235:genetics / Q000302:isolation & purification*; D015398:Signal Transduction; D012989:Soil Pollutants / Q000633:toxicity; D013312:Stress, Physiological; D053719:Tandem Mass Spectrometry
D010940:Plant Proteins; D020543:Proteome; D012989:Soil Pollutants; D003300:Copper