Characterisation of a multidrug-resistant Bacteroides fragilis isolate recovered from blood of a patient in Denmark using whole-genome sequencing.
Here we describe a patient undergoing extensive abdominal surgery and hyperthermic intraperitoneal chemotherapy due to primary adenocarcinoma in the sigmoid colon with peritoneal carcinomatosis. During hospitalisation the patient suffered from bacteraemia with a multidrug-resistant Bacteroides fragilis isolate. Whole-genome sequencing of the isolate resulted in identification of nimE, cfiA and ermF genes corresponding to metronidazole, carbapenem and clindamycin resistance.
Department of Clinical Microbiology, Aarhus University Hospital, Skejby, Brendstrupgårdsvej 100, DK-8200 Aarhus N, Denmark. Electronic address: ninaank3@rm.dk.
Nina
N
Ank
Department of Clinical Microbiology, Odense University Hospital, J.B. Winsløws vej 21.2, DK-5000 Odense C, Denmark.
Thomas V
TV
Sydenham
Department of Surgery P, Aarhus University Hospital, Tage-Hansens Gade 2, DK-8000 Aarhus C, Denmark.
Lene H
LH
Iversen
Department of Clinical Microbiology, Odense University Hospital, J.B. Winsløws vej 21.2, DK-5000 Odense C, Denmark.
Ulrik S
US
Justesen
Department of Clinical Microbiology, Aarhus University Hospital, Skejby, Brendstrupgårdsvej 100, DK-8200 Aarhus N, Denmark.
Mikala
M
Wang
D016470:Bacteremia / Q000382:microbiology*; D001442:Bacteroides Infections / Q000382:microbiology*; D001441:Bacteroides fragilis / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D001769:Blood / Q000382:microbiology*; D003110:Colonic Neoplasms / Q000150:complications; D003718:Denmark; D024901:Drug Resistance, Multiple, Bacterial; D005260:Female; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D006801:Humans; D008875:Middle Aged; D008969:Molecular Sequence Data; D010534:Peritoneal Neoplasms / Q000150:complications / Q000556:secondary; D017422:Sequence Analysis, DNA
Bacteroides fragilis; Multidrug resistance; cfiA; ermF; nimE; tetQ