The Theory and Practice of Genome Sequence Assembly.
The current genomic revolution was made possible by joint advances in genome sequencing technologies and computational approaches for analyzing sequence data. The close interaction between biologists and computational scientists is perhaps most apparent in the development of approaches for sequencing entire genomes, a feat that would not be possible without sophisticated computational tools called genome assemblers (short for genome sequence assemblers). Here, we survey the key developments in algorithms for assembling genome sequences since the development of the first DNA sequencing methods more than 35 years ago.
Ontario Institute for Cancer Research, Toronto, Ontario M5G 0A3, Canada; email: jared.simpson@oicr.on.ca.
Jared T
JT
Simpson
Mihai
M
Pop
D000465:Algorithms; D022202:Chromosomes, Artificial, Bacterial; D003001:Cloning, Molecular; D003196:Computer Graphics; D016678:Genome; D023281:Genomics / Q000379:methods*; D006801:Humans; D017422:Sequence Analysis, DNA / Q000379:methods*
algorithm; bioinformatics; genome sequencing; sequence assembly; shotgun sequencing