Metabolic glycoengineering bacteria for therapeutic, recombinant protein, and metabolite production applications.
Metabolic glycoengineering is a specialization of metabolic engineering that focuses on using small molecule metabolites to manipulate biosynthetic pathways responsible for oligosaccharide and glycoconjugate production. As outlined in this article, this technique has blossomed in mammalian systems over the past three decades but has made only modest progress in prokaryotes. Nevertheless, a sufficient foundation now exists to support several important applications of metabolic glycoengineering in bacteria based on methods to preferentially direct metabolic intermediates into pathways involved in lipopolysaccharide, peptidoglycan, teichoic acid, or capsule polysaccharide production. An overview of current applications and future prospects for this technology are provided in this report.
Department of Biomedical Engineering and the Translational Tissue Engineering Center, The Johns Hopkins University, Baltimore, MD, USA.
Christopher T
CT
Saeui
Department of Biomedical Engineering and the Translational Tissue Engineering Center, The Johns Hopkins University, Baltimore, MD, USA.
Esteban
E
Urias
Department of Biomedical Engineering and the Translational Tissue Engineering Center, The Johns Hopkins University, Baltimore, MD, USA.
Lingshu
L
Liu
Department of Biomedical Engineering and the Translational Tissue Engineering Center, The Johns Hopkins University, Baltimore, MD, USA.
Mohit P
MP
Mathew
Department of Biomedical Engineering and the Translational Tissue Engineering Center, The Johns Hopkins University, Baltimore, MD, USA. kyarema1@jhu.edu.|Translational Tissue Engineering Center, The Johns Hopkins University, 5029 Robert H. & Clarice Smith Building, 400 North Broadway, Baltimore, MD, 21231, USA. kyarema1@jhu.edu.
Kevin J
KJ
Yarema
D000818:Animals; D050260:Carbohydrate Metabolism / Q000235:genetics*; D006001:Glycoconjugates / Q000737:chemistry / Q000378:metabolism; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006031:Glycosylation; D008070:Lipopolysaccharides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D060847:Metabolic Engineering; D009844:Oligosaccharides / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D006001:Glycoconjugates; D006023:Glycoproteins; D008070:Lipopolysaccharides; D009844:Oligosaccharides; D011994:Recombinant Proteins
Carbohydrate engineering; Lipopolysaccharides; Metabolic oligosaccharide engineering; Nucleotide sugar production; Oligosaccharide synthesis; Petidoglycans; Recombinant glycoprotein production