A novel method for screening of potential probiotics for high adhesion capability.
To screen for potential probiotics with high adhesion capability, a chemostat model-based cultured human feces and denaturing gradient gel electrophoresis methods were applied, and the adhesion capability of the isolates was evaluated in vitro and in vivo. Lactobacillus plantarum (HM218749), Lactobacillus reuteri (EU547310), and Enterococcus faecalis (HM218543) were isolated from the slime on the chemostat wall, as these organisms could grow better at 37°C in an anaerobic environment and could resist harsh conditions (pH 1.5 and 0.30% bile salt). Lactobacillus plantarum, L. reuteri, and E. faecalis could adhere to HT-29 cells and reduce the adhesion of Shigella dysenteriae 2457, Staphylococcus aureus Cowan1, Enterobacter sakazakii 45401, and Escherichia coli 44102 to HT-29 cells. Moreover, the animal experiment showed that L. plantarum could adhere to mice intestine, increasing the number of lactobacilli and decreasing the number of enterococci.
Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi 330031, PR China; Institute of Life Science, Nanchang University, Nanchang, Jiangxi 330031, PR China.
Xin
X
Wang
Food and Nutritional Science, School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong.
Qinglong
Q
Wu
Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi 330031, PR China.
Kan
K
Deng
Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi 330031, PR China.
Qiang
Q
Wei
Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi 330031, PR China.
Ping
P
Hu
Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi 330031, PR China.
Jingjing
J
He
Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi 330031, PR China.
Huan
H
Liu
Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi 330031, PR China.
Yong
Y
Zheng
State Key Laboratory of Food Science and Technology, Nanchang University, Jiangxi 330047, China.
Hua
H
Wei
Food and Nutritional Science, School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong. Electronic address: npshah@hku.hk.
Nagendra P
NP
Shah
Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi 330031, PR China. Electronic address: chentingtao1984@163.com.
Tingtao
T
Chen
D001422:Bacterial Adhesion; D019149:Bioreactors; D003106:Colon / Q000382:microbiology; D013293:Enterococcus faecalis / Q000502:physiology*; D005243:Feces / Q000382:microbiology; D000069196:Gastrointestinal Microbiome; D019073:HT29 Cells; D006801:Humans; D048191:Lactiplantibacillus plantarum / Q000502:physiology*; D052200:Limosilactobacillus reuteri / Q000502:physiology*; D019936:Probiotics / Q000032:analysis*
Lactobacillus plantarum; chemostat; denaturing gradient gel electrophoresis; probiotics