Non conventional biological treatment based on Trametes versicolor for the elimination of recalcitrant anticancer drugs in hospital wastewater.
This work presents a study about the elimination of anticancer drugs, a group of pollutants considered recalcitrant during conventional activated sludge wastewater treatment, using a biological treatment based on the fungus Trametes versicolor. A 10-L fluidized bed bioreactor inoculated with this fungus was set up in order to evaluate the removal of 10 selected anticancer drugs in real hospital wastewater. Almost all the tested anticancer drugs were completely removed from the wastewater at the end of the batch experiment (8 days) with the exception of Ifosfamide and Tamoxifen. These two recalcitrant compounds, together with Cyclophosphamide, were selected for further studies to test their degradability by T. versicolor under optimal growth conditions. Cyclophosphamide and Ifosfamide were inalterable during batch experiments both at high and low concentration, whereas Tamoxifen exhibited a decrease in its concentration along the treatment. Two positional isomers of a hydroxylated form of Tamoxifen were identified during this experiment using a high resolution mass spectrometry based on ultra-high performance chromatography coupled to an Orbitrap detector (LTQ-Velos Orbitrap). Finally the identified transformation products of Tamoxifen were monitored in the bioreactor run with real hospital wastewater.
Catalan Institute for Water Research (ICRA), H2O Building, Scientific and Technological Park of the University of Girona, 101-E-17003 Girona, Spain.
Laura
L
Ferrando-Climent
Departament d'Enginyeria Química, Universitat Autònoma de Barcelona (UAB), 08193 Bellaterra, Spain.
Carles
C
Cruz-Morató
Departament d'Enginyeria Química, Universitat Autònoma de Barcelona (UAB), 08193 Bellaterra, Spain.
Ernest
E
Marco-Urrea
Departament d'Enginyeria Química, Universitat Autònoma de Barcelona (UAB), 08193 Bellaterra, Spain.
Teresa
T
Vicent
Departament d'Enginyeria Química, Universitat Autònoma de Barcelona (UAB), 08193 Bellaterra, Spain.
Montserrat
M
Sarrà
Catalan Institute for Water Research (ICRA), H2O Building, Scientific and Technological Park of the University of Girona, 101-E-17003 Girona, Spain.
Sara
S
Rodriguez-Mozaz
Catalan Institute for Water Research (ICRA), H2O Building, Scientific and Technological Park of the University of Girona, 101-E-17003 Girona, Spain; Water and Soil Quality Research Group, Department of Environmental Chemistry, IDAEA-CSIC, Jordi Girona 18-26, 08034 Barcelona, Spain.
Damià
D
Barceló
D000970:Antineoplastic Agents / Q000032:analysis / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology; D006761:Hospitals; D013058:Mass Spectrometry; D012722:Sewage / Q000737:chemistry; D055454:Trametes / Q000378:metabolism*; D062065:Wastewater / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism*
D000970:Antineoplastic Agents; D012722:Sewage; D062065:Wastewater; D014874:Water Pollutants, Chemical
Anticancer drugs; Cytotoxic; HRMS; Hospital effluent; Removal; Trametes versicolor