Novel cyclohexane monooxygenase from Acidovorax sp. CHX100.
Acidovorax sp. CHX100 has a remarkable ability for growth on short cycloalkanes (C5-C8) as a sole source of carbon and energy under aerobic conditions via an uncharacterized mechanism. Transposon mutagenesis of Acidovorax sp. CHX100 revealed a novel cytochrome P450 monooxygenase (CYP450chx) which catalyzed the transformation of cyclohexane to cyclohexanol. Primer walking methods categorized CYP450chx as cytochrome P450 class I taking into account its operon structure: monooxygenase, FAD oxidoreductase, and ferredoxin. CYP450chx was successfully cloned and expressed in Escherichia coli JM109. The activity of CYP450chx was demonstrated by means of the indole co-oxidation. Biotransformation capability of CYP450chx was confirmed through the catalysis of cycloalkanes (C5-C8) to their respective cyclic alcohols.
Department of Biological Waste Air Purification, Institute of Sanitary Engineering, Water Quality and Solid Waste Management - University of Stuttgart, Bandtäle 2, 70569, Stuttgart, Germany.
Diego
D
Salamanca
Rohan
R
Karande
Andreas
A
Schmid
Daniel
D
Dobslaw
D001711:Biotransformation; D003001:Cloning, Molecular; D016000:Cluster Analysis; D042621:Comamonadaceae / Q000201:enzymology* / Q000235:genetics; D003510:Cyclohexanes / Q000378:metabolism*; D003511:Cyclohexanols; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D006899:Mixed Function Oxygenases / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010084:Oxidation-Reduction; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
D003510:Cyclohexanes; D003511:Cyclohexanols; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; C506365:Cyclohexane; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases