Serum α-Tocopherol Has a Nonlinear Inverse Association with Periodontitis among US Adults.
BACKGROUND
Previous experimental models suggest that vitamin E may ameliorate periodontitis. However, epidemiologic studies show inconsistent evidence in supporting this plausible association.


OBJECTIVE
We investigated the association between serum α-tocopherol (αT) and γ-tocopherol (γT) and periodontitis in a large cross-sectional US population.


METHODS
This study included 4708 participants in the 1999-2001 NHANES. Serum tocopherols were measured by HPLC and values were adjusted by total cholesterol (TC). Periodontal status was assessed by mean clinical attachment loss (CAL) and probing pocket depth (PPD). Total periodontitis (TPD) was defined as the sum of mild, moderate, and severe periodontitis. All measurements were performed by NHANES.


RESULTS
Means ± SDs of serum αT:TC ratio from low to high quartiles were 4.0 ± 0.4, 4.8 ± 0.2, 5.7 ± 0.4, and 9.1 ± 2.7 μmol/mmol. In multivariate regression models, αT:TC quartiles were inversely associated with mean CAL (P-trend = 0.06), mean PPD (P-trend < 0.001), and TPD (P-trend < 0.001) overall. Adjusted mean differences (95% CIs) between the first and fourth quartile of αT:TC were 0.12 mm (0.03, 0.20; P-difference = 0.005) for mean CAL and 0.12 mm (0.06, 0.17; P-difference < 0.001) for mean PPD, whereas the corresponding OR for TPD was 1.65 (95% CI: 1.26, 2.16; P-difference = 0.001). In a dose-response analysis, a clear inverse association between αT:TC and mean CAL, mean PPD, and TPD was observed among participants with relatively low αT:TC. No differences were seen in participants with higher αT:TC ratios. Participants with γT:TC ratio in the interquartile range showed a significantly lower mean PPD than those in the highest quartile.


CONCLUSIONS
A nonlinear inverse association was observed between serum αT and severity of periodontitis, which was restricted to adults with normal but relatively low αT status. These findings warrant further confirmation in longitudinal or intervention studies.
Unilever Research and Development, Vlaardingen, The Netherlands;
Geng
G
Zong
Unilever Research and Development, Port Sunlight, Bebington, United Kingdom;
Ann E
AE
Scott
School of Life and Health Sciences, Aston University, Birmingham, United Kingdom; and.
Helen R
http://orcid.org/0000-0002-2666-2147
HR
Griffiths
Unilever Research and Development, Vlaardingen, The Netherlands;
Peter L
PL
Zock
Department of Oral Surgery, School of Dentistry, University of Birmingham, Birmingham, United Kingdom.
Thomas
http://orcid.org/0000-0002-2557-7645
T
Dietrich
Unilever Research and Development, Vlaardingen, The Netherlands; rachel.newson@unilever.com.
Rachel S
http://orcid.org/0000-0001-5603-1875
RS
Newson
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000465:Algorithms; D015415:Biomarkers / Q000097:blood; D002784:Cholesterol / Q000097:blood; D003430:Cross-Sectional Studies; D005260:Female; D006306:Health Surveys; D006801:Humans; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000097:blood / Q000453:epidemiology / Q000209:etiology* / Q000503:physiopathology; D015995:Prevalence; D012720:Severity of Illness Index; D014481:United States / Q000453:epidemiology; D014811:Vitamin E Deficiency / Q000503:physiopathology*; D055815:Young Adult; D024502:alpha-Tocopherol / Q000097:blood*; D024504:gamma-Tocopherol / Q000097:blood
D015415:Biomarkers; D024504:gamma-Tocopherol; D002784:Cholesterol; D024502:alpha-Tocopherol
clinical attachment loss; periodontitis; probing pocket depth; α-tocopherol; γ-tocopherol