Incubation of ovine scrapie with environmental matrix results in biological and biochemical changes of PrP(Sc) over time.
Ovine scrapie can be transmitted via environmental reservoirs. A pool of ovine scrapie isolates were incubated on soil for one day or thirteen months and eluted prion was used to challenge tg338 mice transgenic for ovine PrP. After one-day incubation on soil, two PrP(Sc) phenotypes were present: G338 or Apl338ii. Thirteen months later some divergent PrP(Sc) phenotypes were seen: a mixture of Apl338ii with either G338 or P338, and a completely novel PrP(Sc) deposition, designated Cag338. The data show that prolonged ageing of scrapie prions within an environmental matrix may result in changes in the dominant PrP(Sc) biological/biochemical properties.
ADAS UK, School of Veterinary Medicine and Science, The University of Nottingham, Sutton Bonington Campus, College Road, Sutton Bonington, Leicestershire, UK. Ben.Maddison@adas.co.uk.
Ben C
BC
Maddison
Animal and Plant Health Agency, Woodham Lane, New Haw, Addlestone, Surrey, UK. John.Spiropoulos@apha.gsi.gov.uk.
John
J
Spiropoulos
Animal and Plant Health Agency, Woodham Lane, New Haw, Addlestone, Surrey, UK. Chris.Vickery@apha.gsi.gov.uk.
Christopher M
CM
Vickery
Animal and Plant Health Agency, Woodham Lane, New Haw, Addlestone, Surrey, UK. R.W.Lockey@soton.ac.uk.|Current address: University of Southampton, Southampton, SO17 1BJ, UK. R.W.Lockey@soton.ac.uk.
Richard
R
Lockey
ADAS UK, School of Veterinary Medicine and Science, The University of Nottingham, Sutton Bonington Campus, College Road, Sutton Bonington, Leicestershire, UK. Jonathan.Owen@adas.co.uk.
Jonathan P
JP
Owen
ADAS UK, School of Veterinary Medicine and Science, The University of Nottingham, Sutton Bonington Campus, College Road, Sutton Bonington, Leicestershire, UK. Keith.Bishop@adas.co.uk.
Keith
K
Bishop
ADAS UK, School of Veterinary Medicine and Science, The University of Nottingham, Sutton Bonington Campus, College Road, Sutton Bonington, Leicestershire, UK. Claire.A.Baker@adas.co.uk.
Claire A
CA
Baker
School of Veterinary Medicine and Science, The University of Nottingham, Sutton Bonington Campus, College Road, Sutton Bonington, Leicestershire, UK. kevin.gough@nottingham.ac.uk.
Kevin C
KC
Gough
D000818:Animals; D030801:Animals, Genetically Modified; D004197:Disease Reservoirs / Q000662:veterinary*; D051379:Mice; D018620:PrPSc Proteins / Q000378:metabolism*; D012608:Scrapie / Q000378:metabolism*; D012756:Sheep; D012987:Soil
D018620:PrPSc Proteins; D012987:Soil