Production, optimization and characterization of fibrinolytic enzyme by Bacillus subtilis RJAS19.
The present study aimed at the production, purification and characterization of fibrinolytic nattokinase enzyme from the bacteria isolated from natto food. For the purpose, a fibrinolytic bacterium was isolated and identified as Bacillus subtilis based on 16S rDNA sequence analysis. The strain was employed for the production and optimization of fibrinolytic enzyme. The strain showed better enzyme production during 72nd h of incubation time with 50 degrees C at the pH 9. The lactose and peptone were found to be increasing the enzyme production rate. The enzyme produced was purified and also characterized with the help of SDS-PAGE analysis. The activity and stability profile of the purified enzyme was tested against different temperature and pH. The observations suggesting that the potential of fibrinolytic enzyme produced by Bacillus subtilis RJAS 19 for its applications in preventive medicines.
D J Mukesh
DJ
Kumar
R
R
Rakshitha
M Annu
MA
Vidhya
P Sharon
PS
Jennifer
Sandip
S
Prasad
M Ravi
MR
Kumar
P T
PT
Kalaichelvan
D001412:Bacillus subtilis / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D004269:DNA, Bacterial / Q000235:genetics; D004355:Drug Stability; D004795:Enzyme Stability; D005337:Fibrin / Q000378:metabolism; D005343:Fibrinolytic Agents / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007218:Industrial Microbiology / Q000379:methods*; D007785:Lactose / Q000378:metabolism; D010461:Peptones / Q000378:metabolism; D021521:Ribotyping; D045730:Soy Foods / Q000382:microbiology; D013381:Subtilisins / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D013997:Time Factors
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D005343:Fibrinolytic Agents; D010461:Peptones; D005337:Fibrin; D013381:Subtilisins; C053771:nattokinase; D007785:Lactose