Effect of exogenous xylanase on rumen in vitro gas production and degradability of wheat straw.
The objective of this study was to determine effects of xylanase on in vitro gas production (GP) and in sacco degradability of wheat straw. Rumen fluid was obtained from three Mongolian native goats fitted with permanent rumen cannulas. The trial consisted of five doses (0, 0.5, 1.0, 1.5, 2.0 μL/g of substrate) of a commercial xylanase (Dyadic® xylanase PLUS, Dyadic International, Inc., Jupiter, FL, USA). For the in sacco degradability, different levels of xylanase enzyme were added directly onto 2 g of wheat straw in nylon bags and incubated in the rumen for 3, 6, 12, 24 and 48 h to estimate degradability of wheat straw. Total GP increased (P < 0.001) at all times of incubation at intermediate levels of xylanase. Methane production had a similar pattern at 3 and 12 h of incubation; increased linearly at 24 h of incubation, and was unaffected at 6 and 48 h of incubation. Rumen NH3 -N concentration increased linearly at 3 h and the highest values were observed with intermediate enzyme levels. All ruminal volatile fatty acids increased linearly with intermediate levels of the fibrolytic enzyme. The in sacco rate of dry matter degradation decreased linearly (P = 0.020) with increasing enzymes. Intermediate levels of xylanase improved rumen kinetic fermentation and degradability. The outcome of this research indicated that the application of xylanase enzyme could improve in vitro GP fermentation of wheat straw.
Mongolian State University of Agriculture, Ulaanbaatar, Mongolia.
Norovsambuu
N
Togtokhbayar
Facultad de Medicina Veterinaria y Zootecnia, Universidad Juárez del Estado de Durango, Durango, México.
María A
MA
Cerrillo
CENIDFyMA, INIFAP, Ajuchitlán, México.
Germán Buendía
GB
Rodríguez
Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Estado de México, México.
Mona M M Y
MM
Elghandour
Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Estado de México, México.
Abdelfattah Z M
AZ
Salem
Mongolian State University of Agriculture, Ulaanbaatar, Mongolia.
Chuluunbaatar
C
Urankhaich
Research Institute of Animal Husbandry of Mongolia, Ulaanbaatar, Mongolia.
Sukhbaatar
S
Jigjidpurev
Department of Animal Sciences, Pwani University, Kilifi, Kenya.
Nicholas E
NE
Odongo
Dairy Science Department, National Research Centre, 33 Bohouth st. Dokki, Giza, Egypt.
Ahmed E
AE
Kholif
D000641:Ammonia / Q000378:metabolism; D000821:Animal Feed; D000818:Animals; D004063:Digestion / Q000187:drug effects*; D043364:Endo-1,4-beta Xylanases / Q000008:administration & dosage* / Q000494:pharmacology*; D005232:Fatty Acids, Volatile / Q000096:biosynthesis; D005285:Fermentation / Q000187:drug effects*; D005740:Gases; D006041:Goats; D066298:In Vitro Techniques; D008697:Methane / Q000096:biosynthesis*; D009584:Nitrogen / Q000378:metabolism; D012417:Rumen / Q000378:metabolism* / Q000502:physiology*; D013997:Time Factors; D014908:Triticum
D005232:Fatty Acids, Volatile; D005740:Gases; D000641:Ammonia; D043364:Endo-1,4-beta Xylanases; D009584:Nitrogen; D008697:Methane
degradability; gas production; wheat straw; xylanase