Peri-intraventricular hemorrhage and oxidative and inflammatory stress markers in very-low birth weight newborns.
OBJECTIVE
To evaluate the association between oxidative and inflammatory stress markers with peri-intraventricular hemorrhage (PIVH) in very-low birth weight newborns.


METHODS
This was a prospective study conducted in a level III neonatal unit. Basal and stimulated reactive oxygen intermediates (ROIs), reduced glutathione (GSH), and interleukin-6 (IL-6) levels were measured in umbilical cord blood. Newborns underwent serial ultrasound at the bedside, at 6, 12, 24, and 72hours of life and at seven days for the diagnosis of PIVH, classified as grades I to IV. Two groups were assessed, those with and without PIVH; maternal and neonatal control variables were used for comparison. Univariate and multiple regression analyses were applied.


RESULTS
A total of 125 newborns were assessed. PIVH incidence rate was 12.0%. In the univariate analysis, basal ROI, the use of two or more doses of corticosteroids, birth weight<1,000g, ventilatory support use, and SNAPPE II value ≥ 22 were significantly associated with PIVH. However, in the multivariate analysis, only antenatal steroid use was independently associated with the disease (OR 0,194; 95% CI: 0,048 to 0,773; p=0,02).


CONCLUSIONS
ROI, GSH, and IL-6 levels were not associated with the occurrence of PIVH in very-low birth weight infants.
Neonatology Division, Hospital da Mulher Prof. Dr. José Aristodemo Pinotti, Centro de Atenção Integral à Saúde da Mulher (CAISM), Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil. Electronic address: jamil_pedro@uol.com.br.
Jamil P S
JP
Caldas
Centro de Investigação em Pediatria (CIPED), Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.
Carolina A
CA
Braghini
Centro de Investigação em Pediatria (CIPED), Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.
Taís N
TN
Mazzola
Department of Pediatrics, Faculdade de Ciências Médicas, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.
Maria M S
MM
Vilela
Department of Pediatrics, Faculdade de Ciências Médicas, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.
Sérgio T M
ST
Marba
D000305:Adrenal Cortex Hormones / Q000494:pharmacology; D015415:Biomarkers / Q000097:blood; D002543:Cerebral Hemorrhage / Q000097:blood* / Q000000981:diagnostic imaging; D002552:Cerebral Ventricles / Q000000981:diagnostic imaging; D005260:Female; D005312:Fetal Blood; D005978:Glutathione / Q000097:blood*; D006801:Humans; D007231:Infant, Newborn; D019102:Infant, Very Low Birth Weight; D007249:Inflammation / Q000378:metabolism; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D011446:Prospective Studies; D020011:Protective Agents / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000032:analysis*; D014463:Ultrasonography
D000305:Adrenal Cortex Hormones; D015415:Biomarkers; D015850:Interleukin-6; D020011:Protective Agents; D017382:Reactive Oxygen Species; D005978:Glutathione
Espécies de oxigênio reativas; Estresse oxidativo; Glutathione; Glutationa; Hemorragia cerebral; Interleucina-6; Interleukin-6; Intraventricular hemorrhage; Newborn; Oxidative stress; Reactive oxygen species; Recém-nascido