Thermus amyloliquefaciens sp. nov., isolated from a hot spring sediment sample.
A Gram-stain-negative, aerobic bacterium, designated strain YIM 77409T, was isolated from the Niujie hot spring in the Eryuan county of Dali, Yunnan province, south-west China. Cells of the strain were rod-shaped and colonies were yellow and circular. The strain grew at pH 6.0-8.0 (optimum, pH 7.0) and 50-70°C (optimum, 60-65°C). The predominant menaquinone was MK-8 and the DNA G+C content was 66.4 mol%. Major fatty acids (>10 %) were iso-C15 : 0 and iso-C17 : 0.The polar lipids consisted of one aminophospholipid, one phospholipid and two glycolipids. 16S rRNA gene sequence analysis showed that strain YIM 77409T formed a cluster with Thermus scotoductus DSM 8553T, Thermus antranikianii DSM 12462T, Thermus caliditerrae YIM 77925T and Thermus tengchongensis YIM 77924T, with highest 16S rRNA gene sequence similarity to T. scotoductus DSM 8553T (97.57%). However, DNA-DNA hybridization indicated that strain YIM 77409T should be viewed as a representative of a novel species, as there was only 30.6 ± 1.6% reassociation with T. scotoductus DSM 8553T. On the basis of the morphological and chemotaxonomic characteristics, as well as the genotypic data, it is proposed that strain YIM 77409T represents a novel species of the genus Thermus, with the name Thermus amyloliquefaciens sp. nov. The type strain is YIM 77409T ( = DSM 25898T = KCTC 32024T).
Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, PR China.|State Key Laboratory of Microbial Metabolism and State Key Laboratory of Ocean Engineering, Shanghai Jiao Tong University, Shanghai, 201100, PR China.
Tian-Tian
TT
Yu
Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, PR China.|College of Life Sciences and Technology, Xinxiang Medical University, Xinxiang, 453003, PR China.
Hong
H
Ming
Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, PR China.
Ji-Cheng
JC
Yao
Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, PR China.|State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, PR China.
En-Min
EM
Zhou
Microbial Resource Center, KRIBB, Daejeon 305-806, Republic of Korea.
Dong-Jin
DJ
Park
Bioproducts Research Chair (BRC), College of Science, King Saud University, Riyadh 11451, Kingdom of Saudi Arabia.
Wael N
WN
Hozzein
Microbial Resource Center, KRIBB, Daejeon 305-806, Republic of Korea.
Chang-Jin
CJ
Kim
Bioproducts Research Chair (BRC), College of Science, King Saud University, Riyadh 11451, Kingdom of Saudi Arabia.
Mohammed A M
MAM
Wadaan
Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, PR China.|State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, PR China.
Wen-Jun
WJ
Li
D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology*; D006017:Glycolipids / Q000737:chemistry; D045482:Hot Springs / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013824:Thermus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D006017:Glycolipids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C024223:vitamin MK 8