[The role of Ku antigen in the repair of apurinic/apyrimidinic sites in DNA].
Apurinic/apyrimidinic (AP) sites are some of the most frequent lesions in genomic DNA. It is widely accepted that, irrespective of their origin, AP sites are further processed by the base excision repair (BER) machinery, being the central intermediate of this process. Under special conditions, proteins, which recognize AP sites, are able to form covalent adducts with DNA. By combination of the cross-linking technique with mass-spectrometry analysis, Ku antigen (Ku)--the central player in nonhomologous end joining (NHEJ), the pathway of double-strand break (DSB) repair--was identified as a protein reactive to AP sites. Moreover, Ku was shown to be a 5'-dRP/AP lyase that acts near DSBs in NHEJ. The recent studies have demonstrated involvement of Ku in the different stages of BER. Here, Ku roles in NHEJ and BER pathways of DNA repair are overviewed.
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Kosova
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Lavrik
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Hodyreva
D000818:Animals; D034961:Antigens, Nuclear / Q000737:chemistry / Q000235:genetics*; D001080:Apurinic Acid / Q000737:chemistry / Q000235:genetics; D020134:Catalytic Domain / Q000235:genetics; D018736:DNA Adducts / Q000235:genetics; D059766:DNA End-Joining Repair / Q000235:genetics*; D004260:DNA Repair / Q000235:genetics*; D051747:DNA-Activated Protein Kinase / Q000737:chemistry / Q000235:genetics; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D000072200:Ku Autoantigen; D051379:Mice; D046912:Multiprotein Complexes / Q000737:chemistry / Q000235:genetics; D011119:Polynucleotides / Q000737:chemistry / Q000235:genetics; D055503:Protein Multimerization / Q000235:genetics
D034961:Antigens, Nuclear; D018736:DNA Adducts; D004268:DNA-Binding Proteins; D046912:Multiprotein Complexes; D011119:Polynucleotides; C001790:apyrimidinic acid; D001080:Apurinic Acid; D051747:DNA-Activated Protein Kinase; C000606510:Xrcc6 protein, mouse; D000072200:Ku Autoantigen