Comprehensive Analyses of White-Handed Gibbon Chromosomes Enables Access to 92 Evolutionary Conserved Breakpoints Compared to the Human Genome.
Gibbon species (Hylobatidae) impress with an unusually high number of numerical and structural chromosomal changes within the family itself as well as compared to other Hominoidea including humans. In former studies applying molecular cytogenetic methods, 86 evolutionary conserved breakpoints (ECBs) were reported in the white-handed gibbon (Hylobates lar, HLA) with respect to the human genome. To analyze those ECBs in more detail and also to achieve a better understanding of the fast karyotype evolution in Hylobatidae, molecular data for these regions are indispensably necessary. In the present study, we obtained whole chromosome-specific probes by microdissection of all 21 HLA autosomes and prepared them for aCGH. Locus-specific DNA probes were also used for further molecular cytogenetic characterization of selected regions. Thus, we could map 6 yet unreported ECBs in HLA with respect to the human genome. Additionally, in 26 of the 86 previously reported ECBs, the present approach enabled a more precise breakpoint mapping. Interestingly, a preferred localization of ECBs within segmental duplications, copy number variant regions, and fragile sites was observed.
Institute of Human Genetics, Jena University Hospital, Friedrich Schiller University, Jena, Germany.
Anja
A
Weise
Nadezda
N
Kosyakova
Martin
M
Voigt
Nadine
N
Aust
Kristin
K
Mrasek
Sharon
S
Löhmer
Nikolai
N
Rubtsov
Tatyana V
TV
Karamysheva
Vladimir A
VA
Trifonov
David
D
Hardekopf
Tereza
T
Jančušková
Sona
S
Pekova
Kathleen
K
Wilhelm
Thomas
T
Liehr
Xiaobo
X
Fan
D000818:Animals; D002460:Cell Line; D056905:Chromosome Breakpoints; D002874:Chromosome Mapping; D033481:Chromosomes, Mammalian / Q000235:genetics*; D055028:Comparative Genomic Hybridization; D017124:Conserved Sequence; D019143:Evolution, Molecular; D005260:Female; D015894:Genome, Human / Q000235:genetics*; D006801:Humans; D006922:Hylobates; D059785:Karyotype; D013045:Species Specificity