Effect of microalgae supplementation on the silage quality and anaerobic digestion performance of Manyflower silvergrass.
The silage quality of Manyflower silvergrass with microalgae supplementation was investigated, and the variation in bacterial communities during ensilage period was analyzed by high-throughput sequencing technology. In addition, the specific methane yields of the silages were also evaluated. Results showed that the samples with microalgae supplementation have lower pH value and higher lactic acid concentration (8.0 mg/g FM). Meanwhile, higher NH3-N concentration was observed since microalgae were N-rich material. Microalgae additions also influenced the bacterial community structure. For the samples without microalgae, the major genus was Enterococcus from day 1 to day 3, and then Lactobacillus became the dominant genus. While the prevalent genus represented in the samples with microalgae supplementation was Lactobacillus. The specific methane yields of all samples were within the range of 153 ± 1 mL/g VS to 178 ± 11 mL/g VS and no significant difference was observed.
Guangzhou Institute of Energy Conversion, CAS Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou 510640, China.
Lianhua
L
Li
Guangzhou Institute of Energy Conversion, CAS Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou 510640, China.
Yongming
Y
Sun
Guangzhou Institute of Energy Conversion, CAS Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou 510640, China. Electronic address: yuanzh@ms.giec.ac.cn.
Zhenhong
Z
Yuan
Guangzhou Institute of Energy Conversion, CAS Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou 510640, China.
Xiaoying
X
Kong
Guangzhou Institute of Energy Conversion, CAS Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou 510640, China.
Yao
Y
Wao
Guangzhou Institute of Energy Conversion, CAS Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou 510640, China.
Ligui
L
Yang
Guangzhou Institute of Energy Conversion, CAS Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou 510640, China.
Yi
Y
Zhang
Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.
Dong
D
Li
D000641:Ammonia / Q000032:analysis; D000693:Anaerobiosis; D001419:Bacteria / Q000378:metabolism; D005232:Fatty Acids, Volatile / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008697:Methane / Q000096:biosynthesis; D058086:Microalgae / Q000378:metabolism*; D010802:Phylogeny; D006109:Poaceae / Q000378:metabolism*; D025341:Principal Component Analysis; D012037:Refuse Disposal / Q000379:methods*; D012820:Silage / Q000382:microbiology*
D005232:Fatty Acids, Volatile; D000641:Ammonia; D008697:Methane
Anaerobic digestion; Bacterial community; Manyflower silvergrass; Microalgae; Silage