Akermanite scaffolds reinforced with boron nitride nanosheets in bone tissue engineering.
Akermanite (AKM) is considered to be a promising bioactive material for bone tissue engineering due to the moderate biodegradability and excellent biocompatibility. However, the major disadvantage of AKM is the relatively inadequate fracture toughness, which hinders the further applications. In the study, boron nitride nanosheets (BNNSs) reinforced AKM scaffolds are fabricated by selective laser sintering. The effects of BNNSs on the mechanical properties and microstructure are investigated. The results show that the compressive strength and fracture toughness increase significantly with BNNSs increasing from 0.5 to 1.0 wt%. The remarkable improvement is ascribed to pull out and grain wrapping of BNNSs with AKM matrix. While, overlapping sheets is observed when more BNNSs are added, which results in the decline of mechanical properties. In addition, it is found that the composite scaffolds possess good apatite-formation ability when soaking in simulated body fluids, which have been confirmed by energy dispersed spectroscopy and flourier transform infrared spectroscopy. Moreover, MG63 osteoblast-like cells and human bone marrow stromal cells are seeded on the scaffolds. Scanning electron microscopy analysis confirms that both cells adhere and proliferate well, indicating favorable cytocompatibility. All the facts demonstrate the AKM scaffolds reinforced by BNNSs have potential applications for tissue engineering.
State Key Laboratory of High Performance Complex Manufacturing, Central South University, Changsha, 410083, China.
Cijun
C
Shuai
Zikai
Z
Han
Pei
P
Feng
Chengde
C
Gao
Tao
T
Xiao
Shuping
S
Peng
D018786:Bone Substitutes / Q000138:chemical synthesis*; D016025:Bone Transplantation / Q000295:instrumentation; D001896:Boron Compounds / Q000737:chemistry*; D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line; D049109:Cell Proliferation / Q000502:physiology; D002516:Ceramics / Q000737:chemistry*; D019245:Compressive Strength; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006244:Hardness; D006801:Humans; D008422:Materials Testing; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology*; D010316:Particle Size; D023822:Tissue Engineering / Q000295:instrumentation; D054457:Tissue Scaffolds
D018786:Bone Substitutes; D001896:Boron Compounds; C017282:boron nitride; C023506:Glass ceramics