Pharmacokinetics of cotinine in rats: a potential therapeutic agent for disorders of cognitive function.
BACKGROUND
Attention has been paid to cotinine (COT), one of the major metabolites of nicotine (NIC), for its pro-cognitive effects and potential therapeutic activities against Alzheimer's disease (AD) and other types of cognitive impairment. In order to facilitate pharmacological and toxicological studies on COT for its pro-cognitive activities, we conducted a pharmacokinetic (PK) study of COT in rats, providing important oral and intravenously (iv) PK information.


METHODS
In this study, plasma samples were obtained up to 48 h after COT was dosed to rats orally and iv at a dose of 3mg/kg. Plasma samples were prepared and analyzed using a sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) bioanalytical method, providing concentration profiles of COT and metabolites after oral and iv administrations.


RESULTS
The data were fitted into a one-compartment model and a two-compartment model for the oral and iv groups, respectively, providing important PK information for COT including PK profiles, half-life, clearance and bioavailability. The results suggested fast absorption, slow elimination and high bioavailability of COT in rats.


CONCLUSIONS
Several important facts about the PK properties in rats suggested COT could be a potential pro-cognitive agent. Information about the pharmacokinetics of COT in rats revealed in this study is of great importance for the future studies on COT or potential COT analogs as agents for improving cognition.
Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, The University of Georgia, Athens, USA.
Pei
P
Li
Department of Pharmacology and Toxicology, Georgia Regents University, Augusta, USA.
Wayne D
WD
Beck
Department of Pharmacology and Toxicology, Georgia Regents University, Augusta, USA; Small Animal Behavior Core, Georgia Regents University, Augusta, USA.
Patrick M
PM
Callahan
Department of Pharmacology and Toxicology, Georgia Regents University, Augusta, USA; Small Animal Behavior Core, Georgia Regents University, Augusta, USA.
Alvin V
AV
Terry
Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, The University of Georgia, Athens, USA. Electronic address: mgbart@uga.edu.
Michael G
MG
Bartlett
D061605:Administration, Intravenous; D000284:Administration, Oral; D000818:Animals; D003071:Cognition / Q000187:drug effects* / Q000502:physiology; D003072:Cognition Disorders / Q000097:blood / Q000188:drug therapy*; D003367:Cotinine / Q000008:administration & dosage* / Q000097:blood / Q000493:pharmacokinetics*; D008954:Models, Biological; D051381:Rats; D017208:Rats, Wistar
D003367:Cotinine
Cognition; Cotinine; Hydrophilic interaction chromatography; Pharmacokinetics; Tandem mass spectrometry