First molecular characterisation and expression analysis of a teleost thioredoxin-interacting protein (TXNIP) gene from rock bream (Oplegnathus fasciatus).
Thioredoxin-interacting protein (TXNIP) is an important regulator of glucose metabolism that functions by inhibiting cellular glucose uptake. The full-length rock bream (Oplegnathus fasciatus) TXNIP (RbTXNIP) cDNA (2499 bp) contains an open reading frame of 1188 bp encoding 396 amino acids. Furthermore, multiple alignments showed that the arrestin domain was well conserved among the other TXNIP sequences tested. RbTXNIP was predicted to contain a PxxP and PPxY motif. Phylogenetic analysis indicated that RbTXNIP is most closely related to Fugu rubripes TXNIP. RbTXNIP was expressed significantly in the RBC, intestine, and spleen. RbTXNIP mRNA expression was also examined in several tissues under conditions of bacterial and viral challenge. Generally, all tissues examined from fish infected with Streptococcus iniae, Edwardsiella tarda and red sea bream iridovirus (RSIV) showed significant downregulation in RbTXNIP expression compared to controls. However, RbTXNIP expression showed significant upregulation in the spleen and kidney after injection of recombinant rock bream TRx1 protein. These findings provide a molecular foundation for functional studies and applications in teleosts.
Department of Marine Biology & Aquaculture, College of Marine Science, Gyeongsang National University, 455, Tongyeong 650-160, Republic of Korea.
Ju-Won
JW
Kim
Inland Fisheries Research Institute, NFRDI, Jinhae 645-805, Republic of Korea.
Jung-Ho
JH
Lee
Department of Marine Biology & Aquaculture, College of Marine Science, Gyeongsang National University, 455, Tongyeong 650-160, Republic of Korea.
Jin-Sol
JS
Bae
Biotechnology Research Division, National Fisheries Research and Development Institute, 216 Gijanghaean-ro, Gijang-eup, Gijang-gun, Busan 619-705, Republic of Korea.
Cheul Min
CM
An
Biotechnology Research Division, National Fisheries Research and Development Institute, 216 Gijanghaean-ro, Gijang-eup, Gijang-gun, Busan 619-705, Republic of Korea.
Bo-Hye
BH
Nam
Department of Marine Biology & Aquaculture, College of Marine Science, Gyeongsang National University, 455, Tongyeong 650-160, Republic of Korea.
Ji-Min
JM
Jeong
Department of Marine Biology & Aquaculture, College of Marine Science, Gyeongsang National University, 455, Tongyeong 650-160, Republic of Korea. Electronic address: vinus96@hanmail.net.
Chan-Il
CI
Park
D000818:Animals; D019390:Arrestins / Q000235:genetics; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D003531:Cypriniformes / Q000502:physiology*; D004266:DNA Virus Infections / Q000276:immunology*; D020609:Edwardsiella tarda / Q000276:immunology*; D004756:Enterobacteriaceae Infections / Q000276:immunology*; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007497:Iridoviridae / Q000276:immunology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D013290:Streptococcal Infections / Q000276:immunology*; D013879:Thioredoxins / Q000378:metabolism; D059467:Transcriptome
D019390:Arrestins; D002352:Carrier Proteins; D029941:Fish Proteins; C486715:TXNIP protein, human; D013879:Thioredoxins
Edwardsiella tarda; Red sea bream iridovirus; Rock bream; Streptococcus iniae; Thioredoxin-interacting protein