Elevated Lipoprotein Lipase Activity Does Not Account for the Association Between Adiponectin and HDL in Type 1 Diabetes.
BACKGROUND
Increased high-density lipoprotein cholesterol (HDL-C) is common in type 1 diabetes (T1D) and is associated both with hyperadiponectinemia and with elevated lipoprotein lipase activity (LPL). Because adiponectin has been shown to increase LPL expression, elevated LPL may link the hyperadiponectinemia in T1D with increased HDL.


OBJECTIVE
The purpose of this study was to determine whether LPL activity accounts for the association between adiponectin and HDL in T1D.


METHODS
A cohort of 127 patients with T1D attending the Diabetes Clinic at the University of Miami and 103 healthy control subjects were recruited.


METHODS
HDL-C and adiponectin were measured in the full cohort and in a subgroup, HDL subfractions were obtained by ultracentrifugation, and LPL and hepatic lipase were measured in postheparin plasma.


RESULTS
Total HDL-C and the lowest density HDL subfraction, apolipoprotein A-I, LPL activity, and adiponectin levels were higher in subjects with T1D than in control subjects (P < .05). Both adiponectin and LPL activity were directly associated with total HDL-C and its lowest density subfraction, but adiponectin and LPL were not correlated (P = 0.13). Adiponectin alone explained 11.6% and adiponectin plus LPL explained 23.8% of the HDL-C variance. In a multivariate model, adiponectin remained an independent predictor of HDL-C along with LPL and serum creatinine, explaining together 27% of HDL-C variance.


CONCLUSIONS
Adiponectin was strongly associated with HDL-C in T1D, suggesting that hyperadiponectinemia is linked to the elevated HDL-C in this population. However, this relationship is independent of the association between LPL and HDL-C. Thus, elevated adiponectin and LPL activity are independently related to increased HDL-C in T1D.
Division of Endocrinology, Diabetes and Metabolism (R.M.C., S.D., J.A.L., A.J.M., R.B.G.) and Diabetes Research Institute (A.S., A.J.M., R.B.G.), University of Miami Miller School of Medicine, Miami, Florida 33136; and Health Science Center (T.A.H.), Department of Medicine, University of Tennessee, Memphis, Tennessee 37996.
Rossana M
RM
Calderon
Division of Endocrinology, Diabetes and Metabolism (R.M.C., S.D., J.A.L., A.J.M., R.B.G.) and Diabetes Research Institute (A.S., A.J.M., R.B.G.), University of Miami Miller School of Medicine, Miami, Florida 33136; and Health Science Center (T.A.H.), Department of Medicine, University of Tennessee, Memphis, Tennessee 37996.
Sylvia
S
Diaz
Division of Endocrinology, Diabetes and Metabolism (R.M.C., S.D., J.A.L., A.J.M., R.B.G.) and Diabetes Research Institute (A.S., A.J.M., R.B.G.), University of Miami Miller School of Medicine, Miami, Florida 33136; and Health Science Center (T.A.H.), Department of Medicine, University of Tennessee, Memphis, Tennessee 37996.
Angela
A
Szeto
Division of Endocrinology, Diabetes and Metabolism (R.M.C., S.D., J.A.L., A.J.M., R.B.G.) and Diabetes Research Institute (A.S., A.J.M., R.B.G.), University of Miami Miller School of Medicine, Miami, Florida 33136; and Health Science Center (T.A.H.), Department of Medicine, University of Tennessee, Memphis, Tennessee 37996.
Jose A
JA
Llinas
Division of Endocrinology, Diabetes and Metabolism (R.M.C., S.D., J.A.L., A.J.M., R.B.G.) and Diabetes Research Institute (A.S., A.J.M., R.B.G.), University of Miami Miller School of Medicine, Miami, Florida 33136; and Health Science Center (T.A.H.), Department of Medicine, University of Tennessee, Memphis, Tennessee 37996.
Thomas A
TA
Hughes
Division of Endocrinology, Diabetes and Metabolism (R.M.C., S.D., J.A.L., A.J.M., R.B.G.) and Diabetes Research Institute (A.S., A.J.M., R.B.G.), University of Miami Miller School of Medicine, Miami, Florida 33136; and Health Science Center (T.A.H.), Department of Medicine, University of Tennessee, Memphis, Tennessee 37996.
Armando J
AJ
Mendez
Division of Endocrinology, Diabetes and Metabolism (R.M.C., S.D., J.A.L., A.J.M., R.B.G.) and Diabetes Research Institute (A.S., A.J.M., R.B.G.), University of Miami Miller School of Medicine, Miami, Florida 33136; and Health Science Center (T.A.H.), Department of Medicine, University of Tennessee, Memphis, Tennessee 37996.
Ronald B
RB
Goldberg
D052242:Adiponectin / Q000097:blood*; D000328:Adult; D016022:Case-Control Studies; D008076:Cholesterol, HDL / Q000097:blood*; D015331:Cohort Studies; D003922:Diabetes Mellitus, Type 1 / Q000097:blood* / Q000453:epidemiology; D005260:Female; D006801:Humans; D008071:Lipoprotein Lipase / Q000097:blood* / Q000378:metabolism; D008075:Lipoproteins, HDL / Q000097:blood; D008297:Male; D008875:Middle Aged
D052242:Adiponectin; D008076:Cholesterol, HDL; D008075:Lipoproteins, HDL; D008071:Lipoprotein Lipase