The effect of hyaluronan on the motility of skin dermal fibroblasts in nanofibrous scaffolds.
Nanofibrous scaffolds that use the native extracellular matrix are promising developments in skin tissue regeneration because they provide the proper environment for the adhesion, migration and growth of skin dermal fibroblasts, important during wound healing. In this study, we focus on hyaluronan as a native ECM that regulates cellular motility in nanofibrous scaffolds. PCL/HA nanofibrous scaffolds were generated by electrospinning and assessed for various physicochemical properties. HA-based scaffolds significantly enhanced cell infiltration in vitro and in vivo. The observation of movements in living cells revealed that HA-based scaffolds regulated cell migration speed and direction. This phenomenon may influences by the variation in cell adhesion receptors-integrin β1, and vinculin formation and distribution. Furthermore, we confirmed that HA/CD44 interactions can activate the TGF-β/MMP-2 signaling pathway that promotes cell motility. These findings suggest HA functions in the cell motility of nanofibrous scaffolds and have potential implications for the use of HA-based scaffolds in skin tissue regeneration applications.
Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400030, China.
Yuna
Y
Qian
Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400030, China.
Linhao
L
Li
Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400030, China.
Chao
C
Jiang
Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400030, China.
Wei
W
Xu
Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400030, China.
Yonggang
Y
Lv
Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400030, China.
Li
L
Zhong
Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400030, China.
Kaiyong
K
Cai
Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400030, China. Electronic address: yangli_cqu@163.com.
Li
L
Yang
D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D055664:Electrochemical Techniques; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D018960:Hyaluronan Receptors / Q000235:genetics / Q000378:metabolism; D006820:Hyaluronic Acid / Q000737:chemistry / Q000494:pharmacology*; D019012:Integrin beta1 / Q000235:genetics / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D057139:Nanofibers; D011091:Polyesters / Q000737:chemistry; D061251:Primary Cell Culture; D015398:Signal Transduction; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism; D016596:Vinculin / Q000235:genetics / Q000378:metabolism
C497483:CD44 protein, human; D018960:Hyaluronan Receptors; D019012:Integrin beta1; D011091:Polyesters; D016212:Transforming Growth Factor beta; C488955:VCL protein, human; D016596:Vinculin; C016240:polycaprolactone; D006820:Hyaluronic Acid; D020778:Matrix Metalloproteinase 2
Cell migration; Electrospun; Hyaluronan