Regulation of NUCB2/nesfatin-1 production in rat's stomach and adipose tissue is dependent on age, testosterone levels and lactating status.
Nesfatin-1, which is derived from the NEFA/nucleobindin 2 (NUCB2) precursor, was recently identified as an anorexigenic peptide that is produced in several tissues including the hypothalamus. Currently, no data exist regarding the regulation of NUCB2/nesfatin-1 production in peripheral tissues, such as gastric mucosa and adipose tissue, through different periods of development. The aim of the present work was to study the variations on circulating levels, mRNA expression and tissue content in gastric mucosa and adipose tissue of NUCB2/nesfatin-1 with age and specially in two clue periods of maturation, weaning and puberty. The weaning period affected NUCB2/nesfatin-1 production in gastric tissue. The testosterone changes associated with the initiation of puberty regulated NUCB2/nesfatin-1 production via adipose tissue and gastric NUCB2/nesfatin-1 production. In conclusion, the production of NUCB2/nesfatin-1 by the stomach and adipose tissue fluctuates with age to regulate energy homeostasis during different states of development.
Grupo Fisiopatología Endocrina, Complexo Hospitalario Universitario de Santiago (CHUS/SERGAS).; CIBER Fisiopatología Obesidad y Nutrición, Instituto de Salud Carlos III, Spain; Instituto de Investigación Sanitaria de Santiago de Compostela (IDIS), Santiago de Compostela, Spain.
Lucia L
LL
Senin
CIBER Fisiopatología Obesidad y Nutrición, Instituto de Salud Carlos III, Spain; Department of Physiology, Research Centre of Molecular Medicine and Chronic Diseases (CIMUS), Santiago de Compostela, Spain; Instituto de Investigación Sanitaria de Santiago de Compostela (IDIS), Santiago de Compostela, Spain.
Omar
O
Al-Massadi
Grupo Fisiopatología Endocrina, Complexo Hospitalario Universitario de Santiago (CHUS/SERGAS).; CIBER Fisiopatología Obesidad y Nutrición, Instituto de Salud Carlos III, Spain; Pediatric Department, Universidad de Santiago de Compostela (USC), Santiago de Compostela, Spain; Instituto de Investigación Sanitaria de Santiago de Compostela (IDIS), Santiago de Compostela, Spain.
Silvia
S
Barja-Fernandez
Grupo Fisiopatología Endocrina, Complexo Hospitalario Universitario de Santiago (CHUS/SERGAS).; CIBER Fisiopatología Obesidad y Nutrición, Instituto de Salud Carlos III, Spain; Department of Physiology, Research Centre of Molecular Medicine and Chronic Diseases (CIMUS), Santiago de Compostela, Spain; Instituto de Investigación Sanitaria de Santiago de Compostela (IDIS), Santiago de Compostela, Spain.
Cintia
C
Folgueira
Grupo Fisiopatología Endocrina, Complexo Hospitalario Universitario de Santiago (CHUS/SERGAS).; CIBER Fisiopatología Obesidad y Nutrición, Instituto de Salud Carlos III, Spain; Instituto de Investigación Sanitaria de Santiago de Compostela (IDIS), Santiago de Compostela, Spain.
Cecilia
C
Castelao
CIBER Fisiopatología Obesidad y Nutrición, Instituto de Salud Carlos III, Spain; Department of Physiology, Research Centre of Molecular Medicine and Chronic Diseases (CIMUS), Santiago de Compostela, Spain; Instituto de Investigación Sanitaria de Santiago de Compostela (IDIS), Santiago de Compostela, Spain.
Sulay A
SA
Tovar
Pediatric Department, Universidad de Santiago de Compostela (USC), Santiago de Compostela, Spain; Instituto de Investigación Sanitaria de Santiago de Compostela (IDIS), Santiago de Compostela, Spain.
Rosaura
R
Leis
Cellular and Molecular Cardiology Research Unit and Department of Cardiology, Complexo Hospitalario Universitario de Santiago (CHUS/SERGAS), Santiago de Compostela, Spain; Instituto de Investigación Sanitaria de Santiago de Compostela (IDIS), Santiago de Compostela, Spain.
Francisca
F
Lago
Servicio de Cirugía General, Complexo Hospitalario Universitario de Santiago (CHUS/SERGAS), Santiago de Compostela, Spain.
Javier
J
Baltar
Servicio de Cirugía General, Complexo Hospitalario Universitario de Santiago (CHUS/SERGAS), Santiago de Compostela, Spain.
Ivan
I
Baamonde
CIBER Fisiopatología Obesidad y Nutrición, Instituto de Salud Carlos III, Spain; Department of Physiology, Research Centre of Molecular Medicine and Chronic Diseases (CIMUS), Santiago de Compostela, Spain; Instituto de Investigación Sanitaria de Santiago de Compostela (IDIS), Santiago de Compostela, Spain.
Carlos
C
Dieguez
CIBER Fisiopatología Obesidad y Nutrición, Instituto de Salud Carlos III, Spain; Laboratorio de Endocrinología Molecular y Celular, Universidad de Santiago de Compostela (USC), Santiago de Compostela, Spain; Instituto de Investigación Sanitaria de Santiago de Compostela (IDIS), Santiago de Compostela, Spain.
Felipe F
FF
Casanueva
Grupo Fisiopatología Endocrina, Complexo Hospitalario Universitario de Santiago (CHUS/SERGAS).; CIBER Fisiopatología Obesidad y Nutrición, Instituto de Salud Carlos III, Spain; Instituto de Investigación Sanitaria de Santiago de Compostela (IDIS), Santiago de Compostela, Spain. Electronic address: luisamaria.seoane@usc.es.
Luisa M
LM
Seoane
D000273:Adipose Tissue / Q000378:metabolism*; D000367:Age Factors; D000818:Animals; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004734:Energy Metabolism; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism*; D007774:Lactation / Q000378:metabolism*; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D000080866:Nucleobindins; D051381:Rats; D017207:Rats, Sprague-Dawley; D013739:Testosterone / Q000097:blood*
D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; D009419:Nerve Tissue Proteins; C000630652:Nucb1 protein, rat; D000080866:Nucleobindins; D013739:Testosterone
Adipose tissue; Breastfeeding and puberty; NUCB2/nesfatin-1; Sex; Stomach