Epidemiology of canine leishmaniasis in southern Bahia, Brazil.
Leishmaniosis is a zoonosis caused by protozoa of the genus Leishmania. American cutaneous leishmaniosis (ACL) is mainly caused by the species L. amazonensis and L. braziliensis, and American visceral leishmaniosis (AVL) is caused by L. infantum chagasi. In addition to their proven roles as reservoirs of AVL, dogs are also suspected by researchers to be reservoirs of ACL due to reports of this infection in domestic environments and of infected dogs in endemic areas. The aim of this study was to detect Leishmania sp. infection in dogs from Vila Operária, Buerarema, Bahia, using parasitological tests, indirect immunofluorescent assay (IFA) and polymerase chain reaction (PCR). Furthermore, this study also aimed to identify risk factors associated with illness in dogs in this locality by conducting an epidemiological survey. For this purpose, 292 dogs were clinically evaluated for the presence of skin lesions, and the dogs that showed these changes were submitted to scarification injury to enable preparation of slides for microscopic study of amastigotes. Subsequently, the dogs underwent blood sampling for serological (IFA) and molecular (PCR) tests. Additionally, the owners of the dogs answered an epidemiological questionnaire to facilitate the identification of risk factors for exposure of dogs to pathogens of ACL. Of the 292 dogs studied, 13 (4.5%) had lesions suggestive of ACL, but with a negative parasitological examination and 147 (50.3%) were seropositive according to the IFA. Of the 273 dogs studied using PCR test, 10 (3.66%) were positive for L. braziliensis, and all samples were negative for L. infantum chagasi. Wastelands in the peridomicile and the presence of light in the household were risk factors associated with ACL. The results show that Vila Operária has asymptomatic dogs with ACL and that the detection sensitivity of the IFA was higher than that of PCR for the infected dogs.
Mestre em Ciência Animal, Ilhéus, BA, Brazil.
Nilo Fernandes
NF
Leça Júnior
Doutoranda do Programa de Pós-Graduação em Ciência Animal da Universidade Estadual de Santa Cruz-UESC, Ilhéus, BA, Brazil.
Paula Elisa Brandão
PE
Guedes
Médica Veterinária, Ilhéus, BA, Brazil.
Lailla Nascimento
LN
Santana
Doutorando em Patologia Humana e Experimental no Centro de Pesquisas Gonçalo Moniz (FIOCRUZ-BA), Salvador, BA, Brazil.
Valter dos Anjos
Vdos A
Almeida
Doutorando do Programa de Pós-Graduação em Genética e Biologia Molecular da Universidade Estadual de Santa Cruz-UESC, Ilhéus, BA, Brazil.
Fábio Santos
FS
Carvalho
Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz-UESC-Campus Soane Nazaré de Andrade, Hospital Veterinário, km 16 Rodovia Jorge Amado, CEP 45662-900 Ilhéus, BA, Brazil.
George Rego
GR
Albuquerque
Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz-UESC-Campus Soane Nazaré de Andrade, Hospital Veterinário, km 16 Rodovia Jorge Amado, CEP 45662-900 Ilhéus, BA, Brazil.
Amauri Arias
AA
Wenceslau
Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz-UESC-Campus Soane Nazaré de Andrade, Hospital Veterinário, km 16 Rodovia Jorge Amado, CEP 45662-900 Ilhéus, BA, Brazil.
Alexandre Dias
AD
Munhoz
Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz-UESC-Campus Soane Nazaré de Andrade, Hospital Veterinário, km 16 Rodovia Jorge Amado, CEP 45662-900 Ilhéus, BA, Brazil. Electronic address: fabiana.lessa@gmail.com.
Fabiana Lessa
FL
Silva
D000818:Animals; D058345:Asymptomatic Infections; D001938:Brazil / Q000453:epidemiology; D004283:Dog Diseases / Q000453:epidemiology* / Q000469:parasitology; D004285:Dogs; D019084:Fluorescent Antibody Technique, Indirect; D007891:Leishmania / Q000235:genetics; D016773:Leishmaniasis, Cutaneous / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D007898:Leishmaniasis, Visceral / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D016133:Polymerase Chain Reaction; D015995:Prevalence
Dogs; Indirect immunofluorescence; Leishmania sp.; Polymerase chain reaction; Risk factors