Cellular immunotherapy with ex vivo expanded cord blood T cells in a humanized mouse model of EBV-associated lymphoproliferative disease.
BACKGROUND
Donor lymphocyte infusion is not feasible in recipients of cord blood transplantation.


OBJECTIVE
We investigated whether infusion of T cells expanded from cord blood is effective in the treatment of model mice of Epstein-Barr virus (EBV)-associated lymphoproliferative disease (LPD).


METHODS
Humanized mice with reconstituted human immune system were prepared and LPD was induced by inoculating EBV intravenously. T cells were expanded from the same sample of cord blood as used for generation of humanized mice and infused to EBV-infected humanized mice.


RESULTS
Mice treated with expanded cord blood T cells lived significantly longer than control mice (p = 0.036).


CONCLUSIONS
Infusion of T cells expanded from cord blood was effective in the treatment of model mice for EBV-associated LPD.
Department of Infectious Diseases, National Research Institute for Child Health & Development, 2-10-1 Okura, Setagaya-ku, Tokyo 157-8535, Japan.
Go
G
Matsuda
Ken-ichi
K
Imadome
Fuyuko
F
Kawano
Masashi
M
Mochizuki
Nakaba
N
Ochiai
Tomohiro
T
Morio
Norio
N
Shimizu
Shigeyoshi
S
Fujiwara
D000818:Animals; D049109:Cell Proliferation; D004195:Disease Models, Animal; D020031:Epstein-Barr Virus Infections / Q000276:immunology / Q000628:therapy*; D005260:Female; D005312:Fetal Blood / Q000276:immunology; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D016219:Immunotherapy, Adoptive / Q000379:methods*; D053631:Interleukin Receptor Common gamma Subunit / Q000235:genetics; D008232:Lymphoproliferative Disorders / Q000276:immunology / Q000628:therapy*; D051379:Mice; D018345:Mice, Knockout; D016513:Mice, SCID; D013601:T-Lymphocytes / Q000276:immunology* / Q000637:transplantation / Q000821:virology
C507942:Il2rg protein, mouse; D053631:Interleukin Receptor Common gamma Subunit
Epstein-Barr virus; cord blood transplantation; donor lymphocyte infusion; humanized mouse; lymphoproliferative disease