Genetic characterization of the tick-borne orbiviruses.
The International Committee for Taxonomy of Viruses (ICTV) recognizes four species of tick-borne orbiviruses (TBOs): Chenuda virus, Chobar Gorge virus, Wad Medani virus and Great Island virus (genus Orbivirus, family Reoviridae). Nucleotide (nt) and amino acid (aa) sequence comparisons provide a basis for orbivirus detection and classification, however full genome sequence data were only available for the Great Island virus species. We report representative genome-sequences for the three other TBO species (virus isolates: Chenuda virus (CNUV); Chobar Gorge virus (CGV) and Wad Medani virus (WMV)). Phylogenetic comparisons show that TBOs cluster separately from insect-borne orbiviruses (IBOs). CNUV, CGV, WMV and GIV share low level aa/nt identities with other orbiviruses, in 'conserved' Pol, T2 and T13 proteins/genes, identifying them as four distinct virus-species. The TBO genome segment encoding cell attachment, outer capsid protein 1 (OC1), is approximately half the size of the equivalent segment from insect-borne orbiviruses, helping to explain why tick-borne orbiviruses have a ~1 kb smaller genome.
Vector-borne Viral Diseases Programme, The Pirbright Institute, Ash Road, Pirbright, Woking, Surrey GU24 0NF, UK. manjunatha.belaganahalli@pirbright.ac.uk.
Manjunatha N
MN
Belaganahalli
Department of Animal Biotechnology, LLR University of Veterinary and Animal Sciences, Hisar 125 004, Haryana, India. sushila.maan@pirbright.ac.uk.
Sushila
S
Maan
Department of Animal Biotechnology, LLR University of Veterinary and Animal Sciences, Hisar 125 004, Haryana, India. narendermaan108@gmail.com.
Narender S
NS
Maan
Department of Pathology and Infectious Diseases, Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, Herts AL9 7TA, UK. jbrownlie@rvc.ac.uk.
Joe
J
Brownlie
Department of Pathology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555-0609, USA. rtesh@utmb.edu.
Robert
R
Tesh
Vector-borne Viral Diseases Programme, The Pirbright Institute, Ash Road, Pirbright, Woking, Surrey GU24 0NF, UK. houssam.attoui@pirbright.ac.uk.
Houssam
H
Attoui
Vector-borne Viral Diseases Programme, The Pirbright Institute, Ash Road, Pirbright, Woking, Surrey GU24 0NF, UK. peter.mertens@pirbright.ac.uk.
Peter P C
PP
Mertens
D000818:Animals; D016000:Cluster Analysis; D016679:Genome, Viral; D008969:Molecular Sequence Data; D018163:Orbivirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013987:Ticks / Q000821:virology*
D012367:RNA, Viral