Binding of arabinan or galactan during cellulose synthesis is extensive and reversible.
Arabinans and galactans are major components of the side-chains of pectin in plant cell walls. In order to understand how pectin side-chains interact with cellulose, in this work we studied the interaction of de-branched arabinan (from sugar beet) and linear galactan (from potato) during the synthesis of cellulose by Gluconacetobacter xylinus (ATCC 53524) to mimic in muro assembly. The binding studies reveal that arabinan and galactan are able to bind extensively (>200mg/g of cellulose) during cellulose deposition, and more than pectin (from apple) in the absence of calcium. (13)C NMR revealed that associated arabinan, galactan or apple pectin molecules were neither rigid nor affected cellulose crystallinity, and there was no apparent change in cellulose architecture as reflected in scanning electron micrographs. De-binding of arabinan, galactan or apple pectin occurred as a result of washing, indicating a reversible binding to cellulose, which was modelled in terms of a surface-controlled process. Implications for structural models of primary plant cell walls and possible roles for cellulose binding of arabinan- and galactan-rich pectins in biological processes are discussed.
College of Food Science and Engineering, Northwest A&F University, No. 28 Xinong Road, 712100 Yangling, Shaanxi, China; ARC Centre of Excellence in Plant Cell Walls, Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia 4072, Australia.
Dehui
D
Lin
ARC Centre of Excellence in Plant Cell Walls, Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia 4072, Australia.
Patricia
P
Lopez-Sanchez
ARC Centre of Excellence in Plant Cell Walls, Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia 4072, Australia. Electronic address: mike.gidley@uq.edu.au.
Michael J
MJ
Gidley
D002473:Cell Wall / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism* / Q000648:ultrastructure; D005685:Galactans / Q000378:metabolism*; D041822:Gluconacetobacter xylinus / Q000378:metabolism*; D010368:Pectins / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism*
D005685:Galactans; D011134:Polysaccharides; C030080:araban; D010368:Pectins; D002482:Cellulose
Arabinan; Binding; Cellulose; Galactan; Gluconacetobacter xylinus; Pectin; Plant cell walls