Growth arrest and rapid capture of select pathogens following magnetic nanoparticle treatment.
Thorough understanding of magnetic nanoparticle (MNP) properties is essential for developing new theranostics. In this study, we provide evidence that non-modified magnetic iron oxide nanoparticles and their functionalized derivatives may be used to restrict growth and capture different pathogens. Coprecipitation of Fe(2+) and Fe(3+) ions in an alkaline solution was used to synthesize MNPs that subsequently were functionalized by gold and aminosilane coating. Transmission electron microscopy (TEM), differential scanning calorimetry (DSC) and Fourier transform infrared spectroscopy (FT-IR) were used to assess their physicochemical properties. A significant decrease of Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans outgrown from medium after addition of MNPs or their derivatives was observed during 24h culture. Measurement of optical density revealed that using MNPs, these pathogens can be quickly captured and removed (with efficiency reaching almost 100%) from purposely infected saline buffer and body fluids such as human blood plasma, serum, abdominal fluids and cerebrospinal fluids. These effects depend on nanoparticle concentration, surface chemistry, the type of pathogen, as well as the surrounding environment.
Department of Microbiological and Nanobiomedical Engineering, Medical University of Białystok, Mickiewicza 2c, 15-222 Białystok, Poland; Department of Experimental Pharmacology, Medical University of Białystok, Szpitalna 37, 15-295 Białystok, Poland. Electronic address: mikro.nano@umb.edu.pl.
Katarzyna
K
Niemirowicz
Department of Microbiology, University of Białystok, J. K. Ciołkowski Street 1, 15-245 Białystok, Poland; Laboratory of Applied Microbiology, University of Białystok, J. K. Ciołkowski Street 1, 15-245 Białystok, Poland.
Izabela
I
Swiecicka
Institute of Chemistry, University of Białystok, Hurtowa 1, 15-399 Białystok, Poland.
Agnieszka Z
AZ
Wilczewska
Institute of Chemistry, University of Białystok, Hurtowa 1, 15-399 Białystok, Poland.
Karolina H
KH
Markiewicz
Department of Microbiological and Nanobiomedical Engineering, Medical University of Białystok, Mickiewicza 2c, 15-222 Białystok, Poland.
Urszula
U
Surel
Department of Neurology, Medical University of Białystok, M. Skłodowskiej - Curie 24A, 15-276 Białystok, Poland.
Alina
A
Kułakowska
Department of Physiology, Medical University of Białystok, Mickiewicza 2C, 15-222 Białystok, Poland.
Zbigniew
Z
Namiot
Department of Histology and Embryology, Medical University of Białystok, Waszyngtona 13, 15-269 Białystok, Poland.
Beata
B
Szynaka
Department of Microbiological and Nanobiomedical Engineering, Medical University of Białystok, Mickiewicza 2c, 15-222 Białystok, Poland; The Faculty of Health Sciences of the Jan Kochanowski University in Kielce, IX WiekówKielc 19, 25-317 Kielce, Poland.
Robert
R
Bucki
Department of Experimental Pharmacology, Medical University of Białystok, Szpitalna 37, 15-295 Białystok, Poland.
Halina
H
Car
D002152:Calorimetry, Differential Scanning; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D006046:Gold / Q000737:chemistry; D007501:Iron / Q000737:chemistry; D058185:Magnetite Nanoparticles / Q000737:chemistry / Q000633:toxicity* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development; D012821:Silanes / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development; D013997:Time Factors
D058185:Magnetite Nanoparticles; D012821:Silanes; D006046:Gold; D007501:Iron
Bacteria; Fungi; Magnetic nanoparticles; Separations; Theranostic