Efficient production of a correctly folded mouse α-defensin, cryptdin-4, by refolding during inclusion body solubilization.
Mammalian α-defensins contribute to innate immunity by exerting antimicrobial activity against various pathogens. To perform structural and functional analysis of α-defensins, large amounts of α-defensins are essential. Although many expression systems for the production of recombinant α-defensins have been developed, attempts to obtain large amounts of α-defensins have been only moderately successful. Therefore, in this study, we applied a previously developed aggregation-prone protein coexpression method for the production of mouse α-defensin cryptdin-4 (Crp4) in order to enhance the formation of inclusion bodies in Escherichia coli expression system. By using this method, we succeeded in obtaining a large amount of Crp4 in the form of inclusion bodies. Moreover, we attempted to refold Crp4 directly during the inclusion-body solubilization step under oxidative conditions. Surprisingly, even without any purification, Crp4 was efficiently refolded during the solubilization step of inclusion bodies, and the yield was better than that of the conventional refolding method. NMR spectra of purified Crp4 suggested that it was folded into its correct tertiary structure. Therefore, the method described in this study not only enhances the expression of α-defensin as inclusion bodies, but also eliminates the cumbersome and time-consuming refolding step.
Protein Science Laboratory, Graduate School of Life Science, Hokkaido University, Sapporo, Hokkaido 060-0810, Japan.
Satoshi
S
Tomisawa
Protein Science Laboratory, Graduate School of Life Science, Hokkaido University, Sapporo, Hokkaido 060-0810, Japan.
Yuji
Y
Sato
Protein Science Laboratory, Graduate School of Life Science, Hokkaido University, Sapporo, Hokkaido 060-0810, Japan.
Masakatsu
M
Kamiya
Protein Science Laboratory, Graduate School of Life Science, Hokkaido University, Sapporo, Hokkaido 060-0810, Japan.
Yasuhiro
Y
Kumaki
Protein Science Laboratory, Graduate School of Life Science, Hokkaido University, Sapporo, Hokkaido 060-0810, Japan.
Takashi
T
Kikukawa
Protein Science Laboratory, Graduate School of Life Science, Hokkaido University, Sapporo, Hokkaido 060-0810, Japan.
Keiichi
K
Kawano
Protein Science Laboratory, Graduate School of Life Science, Hokkaido University, Sapporo, Hokkaido 060-0810, Japan.
Makoto
M
Demura
Innate Immunity Laboratory, Graduate School of Life Science, Hokkaido University, Sapporo, Hokkaido 001-0021, Japan.
Kiminori
K
Nakamura
Innate Immunity Laboratory, Graduate School of Life Science, Hokkaido University, Sapporo, Hokkaido 001-0021, Japan.
Tokiyoshi
T
Ayabe
Protein Science Laboratory, Graduate School of Life Science, Hokkaido University, Sapporo, Hokkaido 060-0810, Japan. Electronic address: aizawa@mail.sci.hokudai.ac.jp.
Tomoyasu
T
Aizawa
D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D002852:Chromatography, Ion Exchange; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics*; D015870:Gene Expression; D002479:Inclusion Bodies / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D019906:Nuclear Magnetic Resonance, Biomolecular; D058849:Protein Refolding; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D012995:Solubility; D023084:alpha-Defensins / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D011994:Recombinant Proteins; D023084:alpha-Defensins; C401847:cryptdin 4, mouse
Coexpression; Inclusion bodies; NMR; Refolding; α-Defensin