Efficient one-pot synthesis of trans-Pt(II)(salicylaldimine)(4-picoline)Cl complexes: effective agents for enhanced expression of p53 tumor suppressor genes.
A series of trans-Pt(II)(salicylaldimine)(4-picoline)Cl complexes were synthesized in 78-87% yield using a one-pot procedure from commercially available precursors. The structures of these complexes were characterized by (1)H, (19)F and (13)C NMR spectroscopy, HRMS (ESI) as well as single crystal X-ray analysis. Bioactivity investigations including bio-assay, time- and dose-dependent, cell cycle progression study, caspase 3 and 9 apoptosis marker assay and DNA interaction using pBR322 plasmid DNA by gel electrophoresis were performed. The results indicated that the complexes showed promising in vitro cytotoxicity in MCF-7 and A549 cancer cell lines. Moreover these complexes enhanced the expression of p53 tumor suppressor gene family members such as p63 and p73.
Department of Chemistry, Fudan University, 220 Handan Road, Shanghai 200433, China. zhangdw@fudan.edu.cn wanghui@fudan.edu.cn.
Faiz-Ur
FU
Rahman
Amjad
A
Ali
Rong
R
Guo
Wei-Kun
WK
Wang
Hui
H
Wang
Zhan-Ting
ZT
Li
Yuejian
Y
Lin
Dan-Wei
DW
Zhang
D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D009944:Organoplatinum Compounds / Q000737:chemistry* / Q000494:pharmacology*; D012459:Salicylates / Q000737:chemistry; D016159:Tumor Suppressor Protein p53 / Q000235:genetics
D000970:Antineoplastic Agents; D009944:Organoplatinum Compounds; D012459:Salicylates; D016159:Tumor Suppressor Protein p53; D004247:DNA; D053148:Caspase 3; D053453:Caspase 9