Dissecting the fungal biology of Bipolaris papendorfii: from phylogenetic to comparative genomic analysis.
Bipolaris papendorfii has been reported as a fungal plant pathogen that rarely causes opportunistic infection in humans. Secondary metabolites isolated from this fungus possess medicinal and anticancer properties. However, its genetic fundamental and basic biology are largely unknown. In this study, we report the first draft genome sequence of B. papendorfii UM 226 isolated from the skin scraping of a patient. The assembled 33.4 Mb genome encodes 11,015 putative coding DNA sequences, of which, 2.49% are predicted transposable elements. Multilocus phylogenetic and phylogenomic analyses showed B. papendorfii UM 226 clustering with Curvularia species, apart from other plant pathogenic Bipolaris species. Its genomic features suggest that it is a heterothallic fungus with a putative unique gene encoding the LysM-containing protein which might be involved in fungal virulence on host plants, as well as a wide array of enzymes involved in carbohydrate metabolism, degradation of polysaccharides and lignin in the plant cell wall, secondary metabolite biosynthesis (including dimethylallyl tryptophan synthase, non-ribosomal peptide synthetase, polyketide synthase), the terpenoid pathway and the caffeine metabolism. This first genomic characterization of B. papendorfii provides the basis for further studies on its biology, pathogenicity and medicinal potential.
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia.
Chee Sian
CS
Kuan
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia.
Su Mei
SM
Yew
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia.
Yue Fen
YF
Toh
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia.
Chai Ling
CL
Chan
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia.
Yun Fong
YF
Ngeow
Codon Genomics SB, Jalan Bandar Lapan Belas, Selangor Darul Ehsan 47160, Malaysia.
Kok Wei
KW
Lee
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia.
Shiang Ling
SL
Na
Codon Genomics SB, Jalan Bandar Lapan Belas, Selangor Darul Ehsan 47160, Malaysia.
Wai-Yan
WY
Yee
Codon Genomics SB, Jalan Bandar Lapan Belas, Selangor Darul Ehsan 47160, Malaysia.
Chee-Choong
CC
Hoh
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia kpng@ummc.edu.my.
Kee Peng
KP
Ng
D001203:Ascomycota / Q000235:genetics / Q000502:physiology*; D004251:DNA Transposable Elements; D016681:Genome, Fungal; D023281:Genomics; D010802:Phylogeny; D064210:Secondary Metabolism / Q000235:genetics
D004251:DNA Transposable Elements
Bipolaris papendorfii; Cochliobolus; Curvularia; LysM domain; dematiaceous