[In vitro study of electrospun polycaprolactone/chitosan nano fiber membrane guided bone regeneration].
OBJECTIVE
To explore the effect of electrospining chitosan/polycaprolactone nano fiber membrane on regeneration of bone defects in vitro.


METHODS
Chitosan/polycaprolactone nanofiber membrane was prepared by electrospinning technique. According to alkaline phosphatase activity and Western blot results, the ability of osteogenic differentiation of cells by fiber membrane as carrier was evaluated. The data was analyzed statistically with SPSS17.0 software package.


RESULTS
The osteogenic differentiation capacity of electrospining chitosan/polycaprolactone nano fiber membrane was significantly higher than the control (without materials), and the difference was significant (P<0.05).


CONCLUSIONS
Electrospinning chitosan/polycaprolactone nano fiber membrane can promote osteogenic differentiation of cells in vitro, which provides a scientific basis for clinical regeneration of bone defects.
Stomatologic Hospital & College, Anhui Medical University; Key Lab of Oral Diseases Research of Anhui Province. Hefei 230032, Anhui Province.China.E-mail:wwhy2013@126.com.
Ying
Y
He
Xin
X
Liu
Xu-yan
XY
Tang
D001861:Bone Regeneration; D002454:Cell Differentiation; D048271:Chitosan; D006801:Humans; D066298:In Vitro Techniques; D057139:Nanofibers; D010012:Osteogenesis; D011091:Polyesters
D011091:Polyesters; C016240:polycaprolactone; D048271:Chitosan