First evidence of molecular characterization of rohu carp Sox2 gene being expressed in proliferating spermatogonial cells.
Because little is known about the function of Sox2 (Sry-related box-2) in teleosts, the objective of this study was to clone and characterize Sox2 complementary DNA (cDNA) from the testis of Indian major carp, Labeo rohita (rohu). The full-length cDNA contained an open reading frame of 936 nucleotides bearing the typical structural features. Phylogenetically, Sox2 of L rohita was most closely related to freshwater counterparts than marine water. The sequence information of cDNA and genomic DNA together revealed that the Sox2 gene is encoded by an uninterrupted exon. Furthermore, comparative mRNA expression profile in various organs including proliferating spermatogonial stem cells (SSCs) suggested about the participatory role of Sox2 during fish male germ cell development and maintenance of stem cells. In support, we have also provided evidence that Sox2 protein is indeed present in rohu SSCs by Western blot analysis. The evolutionarily conserved high-mobility group box domain indicated its possible involvement in common networking pathways for stem cell maintenance and pluripotency between mammals and nonmammals. Our findings could be the first step toward the use of Sox2 as a potential biomarker for proliferating SSCs and understanding the transcriptional regulatory network involved during male germ cell development and maintenance in fish species.
Fish Genetics and Biotechnology Division, ICAR-Central Institute of Freshwater Aquaculture, Indian Council of Agricultural Research (ICAR), Bhubaneswar, Odisha, India.
Swagat Kumar
SK
Patra
Fish Genetics and Biotechnology Division, ICAR-Central Institute of Freshwater Aquaculture, Indian Council of Agricultural Research (ICAR), Bhubaneswar, Odisha, India.
Vemulawada
V
Chakrapani
Fish Genetics and Biotechnology Division, ICAR-Central Institute of Freshwater Aquaculture, Indian Council of Agricultural Research (ICAR), Bhubaneswar, Odisha, India.
Rudra Prasanna
RP
Panda
Fish Genetics and Biotechnology Division, ICAR-Central Institute of Freshwater Aquaculture, Indian Council of Agricultural Research (ICAR), Bhubaneswar, Odisha, India.
Chinmayee
C
Mohapatra
Fish Genetics and Biotechnology Division, ICAR-Central Institute of Freshwater Aquaculture, Indian Council of Agricultural Research (ICAR), Bhubaneswar, Odisha, India.
Pallipuram
P
Jayasankar
Fish Genetics and Biotechnology Division, ICAR-Central Institute of Freshwater Aquaculture, Indian Council of Agricultural Research (ICAR), Bhubaneswar, Odisha, India. Electronic address: hkbarman68@hotmail.com.
Hirak Kumar
HK
Barman
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000378:metabolism*; D049109:Cell Proliferation; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D029941:Fish Proteins / Q000235:genetics*; D015870:Gene Expression; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000032:analysis; D055747:SOX Transcription Factors / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000662:veterinary; D013093:Spermatogonia / Q000737:chemistry / Q000378:metabolism*; D013737:Testis / Q000737:chemistry; D059467:Transcriptome
D018076:DNA, Complementary; D029941:Fish Proteins; D012333:RNA, Messenger; D055747:SOX Transcription Factors
High-mobility group box domain; Labeo rohita; Rohu; Sox2; Spermatogonial stem cell