Athrombogenic hydrogel coatings for medical devices--Examination of biological properties.
In the article the authors present hydrogel coatings prepared from polyvinylpyrrolidone (PVP) macromolecules, which are chemically bonded to polyurethane (PU) substrate. The coating is designed to improve the surface hemocompatibility of blood-contacting medical devices. The coating was characterized in terms of physical properties (swelling ratio, hydrogel density, surface morphology, coating thickness, coating durability). In order to examine surface hemocompatibility, the materials were contacted with whole human blood under arterial flow simulated conditions followed by calculation of platelet consumption and the number of platelet aggregates. Samples were also contacted with platelet-poor plasma; the number of surface-adsorbed fibrinogen molecules was measured using ELISA assay. Finally, the inflammatory reaction after implantation was assessed, using New Zealand rabbits. The designed coating is characterized by high water content and excellent durability in aqueous environment - over a 35-day period, no significant changes in coating thickness were observed. Experiments with blood proved twice the reduction in adsorption of serum-derived fibrinogen together with a moderate reduction in the number of platelet aggregates formed during the contact of the material with blood. The analysis of an inflammatory reaction after the implantation confirmed high biocompatibility of the fabricated materials - studies have shown no toxic effects of the implanted material on the surrounding animal tissues.
Laboratory of Biomedical Engineering, Faculty of Chemical and Process Engineering, Warsaw University of Technology, Waryńskiego 1, 00-645 Warsaw, Poland. Electronic address: b.butruk@ichip.pw.edu.pl.
Beata A
BA
Butruk-Raszeja
Laboratory of Biomedical Engineering, Faculty of Chemical and Process Engineering, Warsaw University of Technology, Waryńskiego 1, 00-645 Warsaw, Poland.
Ilona
I
Łojszczyk
Laboratory of Biomedical Engineering, Faculty of Chemical and Process Engineering, Warsaw University of Technology, Waryńskiego 1, 00-645 Warsaw, Poland.
Tomasz
T
Ciach
Artificial Heart Laboratory, Foundation for Cardiac Surgery Development, Wolności 345A, 41-800 Zabrze, Poland.
Magdalena
M
Kościelniak-Ziemniak
Artificial Heart Laboratory, Foundation for Cardiac Surgery Development, Wolności 345A, 41-800 Zabrze, Poland.
Karolina
K
Janiczak
Artificial Heart Laboratory, Foundation for Cardiac Surgery Development, Wolności 345A, 41-800 Zabrze, Poland.
Roman
R
Kustosz
Artificial Heart Laboratory, Foundation for Cardiac Surgery Development, Wolności 345A, 41-800 Zabrze, Poland.
Małgorzata
M
Gonsior
D000327:Adsorption; D000818:Animals; D001792:Blood Platelets / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000494:pharmacology; D005340:Fibrinogen / Q000737:chemistry; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D008422:Materials Testing; D023421:Models, Animal; D010973:Platelet Adhesiveness / Q000187:drug effects; D011140:Polyurethanes / Q000737:chemistry*; D011205:Povidone / Q000737:chemistry*; D019736:Prostheses and Implants; D011817:Rabbits; D013499:Surface Properties
D020099:Coated Materials, Biocompatible; D011140:Polyurethanes; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D005340:Fibrinogen; D011205:Povidone
Blood compatibility; Fibrinogen; Hydrogel; Platelet; Polyurethane; Surface modification