Bovine serum albumin-sodium alkyl sulfates bioconjugates as drug delivery systems.
Precipitation of bovine serum albumin (BSA) by anionic surfactants with alkyl chains of increasing lengths (octyl, decyl, dodecyl sulfates) was studied at room temperature, at pH 3.0, in isotonic sodium chloride solution. The particle size of albumin, the zeta potential, the surface charge and fluorescent properties of BSA-surfactant composites were investigated concerning addition of increasing amount of surfactant. The thermal stability of the systems was monitored by calorimetric analysis (DSC). The formation of the well-ordered structure in the self-assembly process in liquid phase was studied by XRD measurement. The structure of the precipitated BSA-surfactant nanocomposites was characterized by small-angle X-ray scattering (SAXS). Finally, ibuprofen (IBU) molecules were enclosed in BSA-surfactant bioconjugate systems and the release properties of the drug were investigated. It has been found out that, as a consequence to the increasing number of carbon atoms in the alkyl chains of the surfactant, the structure and the fluorescent properties of the aggregates formed can be controlled due to the increase in the hydrophobicity of BSA-surfactant composites. The bioconjugates are well applicable as carrier to realize controlled release of drug molecules.
MTA-SZTE Supramolecular and Nanostructured Materials Research Group, University of Szeged, H-6720 Dóm tér. 8, Szeged, Hungary.
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Benkő
MTA-SZTE Supramolecular and Nanostructured Materials Research Group, University of Szeged, H-6720 Dóm tér. 8, Szeged, Hungary.
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Varga
MTA-SZTE Supramolecular and Nanostructured Materials Research Group, University of Szeged, H-6720 Dóm tér. 8, Szeged, Hungary.
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Sebők
MTA-SZTE Supramolecular and Nanostructured Materials Research Group, University of Szeged, H-6720 Dóm tér. 8, Szeged, Hungary.
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Bohus
Department of Physical Chemistry and Materials Science, Faculty of Science and Informatics, University of Szeged, H-6720 Aradi vt. 1, Szeged, Hungary.
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Juhász
MTA-SZTE Supramolecular and Nanostructured Materials Research Group, University of Szeged, H-6720 Dóm tér. 8, Szeged, Hungary; Department of Medical Chemistry, Faculty of Medicine, University of Szeged, H-6720 Dóm tér. 8, Szeged, Hungary. Electronic address: i.dekany@chem.u-szeged.hu.
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Dékány
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D002151:Calorimetry / Q000379:methods; D002417:Cattle; D016503:Drug Delivery Systems / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007052:Ibuprofen / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D007700:Kinetics; D008956:Models, Chemical; D008958:Models, Molecular; D053838:Scattering, Small Angle; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry*; D014961:X-Ray Diffraction
D000894:Anti-Inflammatory Agents, Non-Steroidal; D013501:Surface-Active Agents; D012710:Serum Albumin, Bovine; D012967:Sodium Dodecyl Sulfate; D007052:Ibuprofen
Anionic surfactants; BSA bioconjugates; Ibuprofen release; Kinetic models; SAXS