G&T-seq: parallel sequencing of single-cell genomes and transcriptomes.
The simultaneous sequencing of a single cell's genome and transcriptome offers a powerful means to dissect genetic variation and its effect on gene expression. Here we describe G&T-seq, a method for separating and sequencing genomic DNA and full-length mRNA from single cells. By applying G&T-seq to over 220 single cells from mice and humans, we discovered cellular properties that could not be inferred from DNA or RNA sequencing alone.
Sanger Institute-EBI Single-Cell Genomics Centre, Wellcome Trust Sanger Institute, Hinxton, UK.
Iain C
IC
Macaulay
MRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK.
Wilfried
W
Haerty
Department of Human Genetics, University of Leuven, Leuven, Belgium.
Parveen
P
Kumar
MRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK.
Yang I
000000020736251X
YI
Li
MRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK.
Tim Xiaoming
TX
Hu
Cancer Genome Project, Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.
Mabel J
MJ
Teng
Department of Physiology, Development and Neuroscience, Downing Site, University of Cambridge, Cambridge, UK.
Mubeen
M
Goolam
Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, UK.
Nathalie
N
Saurat
Sequencing R&D, Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.
Paul
P
Coupland
Sequencing R&D, Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.
Lesley M
LM
Shirley
Sequencing R&D, Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.
Miriam
M
Smith
Department of Human Genetics, University of Leuven, Leuven, Belgium.
Niels
N
Van der Aa
Cytogenetics Core Facility, Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.
Ruby
R
Banerjee
Sequencing R&D, Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.
Peter D
PD
Ellis
Sequencing R&D, Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.
Michael A
MA
Quail
Sequencing R&D, Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.
Harold P
HP
Swerdlow
Department of Physiology, Development and Neuroscience, Downing Site, University of Cambridge, Cambridge, UK.
Magdalena
M
Zernicka-Goetz
Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, UK.
Frederick J
FJ
Livesey
1] Sanger Institute-EBI Single-Cell Genomics Centre, Wellcome Trust Sanger Institute, Hinxton, UK. [2] MRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK.
Chris P
CP
Ponting
1] Sanger Institute-EBI Single-Cell Genomics Centre, Wellcome Trust Sanger Institute, Hinxton, UK. [2] Department of Human Genetics, University of Leuven, Leuven, Belgium.
Thierry
T
Voet
D000818:Animals; D045744:Cell Line, Tumor; D004247:DNA / Q000235:genetics*; D023281:Genomics / Q000379:methods*; D006801:Humans; D051379:Mice; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics*
D012333:RNA, Messenger; D004247:DNA