Fluorescent oligo(p-phenyleneethynylene) contained amphiphiles-encapsulated magnetic nanoparticles for targeted magnetic resonance and two-photon optical imaging in vitro and in vivo.
Folate receptor-targeted multifunctional fluorescent magnetic nanoparticles (FMNPs) composed of cores containing iron oxide nanocrystals and amphiphilic oligo(p-phenyleneethynylene) shells with multimodal imaging capability were successfully prepared through a convenient hydrophobic encapsulation approach. The iron oxide nanoparticles in the core provided T2-weighted magnetic resonance imaging (MRI), whereas the amphiphilic oligomers on the surface of the nanoparticles introduced good water-solubility, biocompatibility, excellent fluorescent properties and cancer-targeting. These nanoparticles exhibited superparamagnetic properties with saturation magnetization (Ms) of 23 emu g(-1) and a transverse relaxivity rate of 140.89 mM(-1) s(-1). In vitro studies indicated that the dual-modal FMNPs can serve as an effective two-photon fluorescent and a magnetic probe to achieve the targeted imaging of Hela cells without obvious cytotoxicity. In vivo two-photon fluorescence and MRI results demonstrated that the FMNPs were able to preferentially accumulate in tumor tissues to allow dual-modal detection of tumors in a living body. These studies provided insight in developing novel multifunctional probes for multimodal imaging, which would play an important role for theranostics in biomedical science.
Key Laboratory for Organic Electronics and Information Displays & Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts & Telecommunications, 9 Wenyuan Road, Nanjing 210023, China. iamqlfan@njupt.edu.cn.
Chao
C
Yin
Binbin
B
Hong
Zhaocui
Z
Gong
Hui
H
Zhao
Wenbo
W
Hu
Xiaomei
X
Lu
Jie
J
Li
Xiang
X
Li
Zhen
Z
Yang
Quli
Q
Fan
Yuyu
Y
Yao
Wei
W
Huang
D000480:Alkynes / Q000737:chemistry*; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D004987:Ethers / Q000737:chemistry*; D052203:Ferrosoferric Oxide / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D018613:Microscopy, Confocal; D041681:NIH 3T3 Cells; D009369:Neoplasms / Q000000981:diagnostic imaging* / Q000473:pathology; D017785:Photons; D011092:Polyethylene Glycols / Q000737:chemistry; D011859:Radiography; D014183:Transplantation, Heterologous
D000480:Alkynes; D004987:Ethers; D005456:Fluorescent Dyes; D058185:Magnetite Nanoparticles; C436384:phenyleneethynylene; D011092:Polyethylene Glycols; D052203:Ferrosoferric Oxide