Regulation of neutrophil infiltration into peritoneal cavity by laparoscopic gastrectomy.
OBJECTIVE
Laparoscopic surgery is a minimally invasive operation developed for treating gastrointestinal malignancies. We aimed to characterize the differences in the intra-abdominal environment following open and laparoscopic surgeries.


METHODS
We investigated data of 48 patients who underwent gastrectomy between 2010 and 2012. We analyzed the mRNA expression of chemokines, indoleamine 2, 3-dioxygenase (IDO), and so on in peritoneal lavage fluid with real-time RT-PCR. We also determined the leukocyte population and calculated the granulocyte/lymphocyte (G/L) ratio in peritoneal lavage fluid using flow cytometry.


RESULTS
CCL3 mRNA was significantly upregulated, whereas IDO mRNA was significantly downregulated, in the open group compared to the laparoscopic surgery group. Flow cytometry revealed that the G/L ratio was significantly higher in the open group.


CONCLUSIONS
We suggest that the production of chemokines and neutrophil infiltration into the abdominal cavity may be suppressed in the laparoscopic surgery. Thus, laparoscopic surgery may be beneficial in preserving local immunity.
Mami
M
Yoshii
Hiroaki
H
Tanaka
Masaichi
M
Ohira
Kazuya
K
Muguruma
Tomohiro
T
Lee
Katsunobu
K
Sakurai
Naoshi
N
Kubo
Kiyoshi
K
Maeda
Kosei
K
Hirakawa
D000368:Aged; D054405:Chemokine CCL3 / Q000235:genetics; D005260:Female; D005434:Flow Cytometry; D005743:Gastrectomy / Q000009:adverse effects / Q000379:methods*; D005786:Gene Expression Regulation; D006801:Humans; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000235:genetics; D007249:Inflammation / Q000235:genetics / Q000276:immunology / Q000517:prevention & control*; D010535:Laparoscopy / Q000009:adverse effects; D008297:Male; D008875:Middle Aged; D020556:Neutrophil Infiltration; D010529:Peritoneal Cavity / Q000166:cytology*; D010533:Peritoneal Lavage; D012333:RNA, Messenger / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000473:pathology / Q000601:surgery*; D016896:Treatment Outcome
C515429:CCL3 protein, human; D054405:Chemokine CCL3; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D012333:RNA, Messenger