Effect of chrysin on nociception in formalin test and serum levels of noradrenalin and corticosterone in rats.
OBJECTIVE
The levels of corticosterone and noradrenalin as the two nociception modulators modify after stress condition. The propose of current study was to investigate the effect of chrysin on formalin-induced nociceptive behaviors and serum levels of corticosterone and noradrenalin in rats.


METHODS
Pain was induced by applying 20 μL of 5% formalin in distilled water in the subplantar of the right hind paw. Chrysin (50, 100 and 150 mg/kg, intraperitoneally (i.p.) was administered 60 min before formalin injection. Morphine (10 mg/kg, i.p.) was administered 30 min before formalin injection. The control group received the same volume of saline by i.p. injection 30 min before formalin injection.


RESULTS
Chrysin treatment can significantly decrease formalin-induced pain in rat in a dose-dependent manner. Chrysin (150 mg/kg) significantly inhibit the first phase (P < 0.01), whereas, the all concentration of chrysin were affected on the later phase of formalin-induced pain (P < 0.05). Chrysin could significantly attenuate the content of corticosterone and noradrenalin in the serum versus to the control rats (P < 0.01).


CONCLUSIONS
The current study confirms that the chrysin decreased the nociceptive behaviors in the formalin test and indicate a correlation with decrease in serum corticosterone and noradrenalin levels.
Department of Agriculture, Payam Noor University P. O. Box 19395-4697 Tt Tehran, Iran.
Tahereh
T
Farkhondeh
Department of Basic Medical Sciences, Neyshabur University of Medical Sciences Neyshabur, Iran.
Saeed
S
Samarghandian
Department of Genetic, Faculty of Medicine, Mashhad University of Medical Sciences Iran.
Mohsen
M
Azimin-Nezhad
Department of Neurosurgery, Faculty of Medicine, Mashhad University of Medical Sciences Iran.
Fariborz
F
Samini
Chrysin; corticosterone; nociception; noradrenalin