A novel albumin nanocomplex containing both small interfering RNA and gold nanorods for synergetic anticancer therapy.
Therapeutic nanocomplexes have been extensively developed for the effective treatment of aggressive cancers because of their outstanding versatility, easy manipulation, and low cytotoxicity. In this study, we describe the synthesis of a novel bovine serum albumin (BSA)-based nanocomplex harboring both Bcl-2-specific small interfering RNA (siRNA) and gold (Au) nanorods (siRNA and rods encapsulated in BSA; SREB) with the aim of developing a targeted breast cancer therapeutic. The SREB complexes contained 2 × 10(5) siRNA molecules and eight Au nanorods per BSA complex and were successively functionalized with polyethylene glycol (PEG) and anti-ErbB-2 antibodies to facilitate active targeting. The synergetic therapeutic activity originating from the two components effectively induced cell death (∼80% reduction in viability compared with control cells) in target breast cancer cells after a single dose of laser irradiation. Intracellular SREB nanocomplex decomposition by proteolytic enzymes resulted in simultaneous RNA interference and thermal ablation, thus leading to apoptosis in the targeted cancer cells. Moreover, these therapeutic effects were sustained for approximately 72 hours. The intrinsic biocompatibility, multifunctionality, and potent in vitro anticancer properties of these SREB nanocomplexes indicate that they have great therapeutic potential for in vivo targeted cancer therapy, in addition to other areas of nanomedicine.
Department of Chemical & Biomolecular Engineering, Sogang University, 35 Baekbeom-Ro, Mapo-gu, Seoul 121-742, Republic of Korea. bkoh@sogang.ac.kr.
Jin-Ha
JH
Choi
Hai-Jin
HJ
Hwang
Seung Won
SW
Shin
Jeong-Woo
JW
Choi
Soong Ho
SH
Um
Byung-Keun
BK
Oh
D000818:Animals; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D002417:Cattle; D005260:Female; D006046:Gold / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D061986:MCF-7 Cells; D043942:Nanotubes / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000737:chemistry / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000494:pharmacology
D034741:RNA, Small Interfering; D012710:Serum Albumin, Bovine; D006046:Gold