PCR-based assay for the mitochondrial cox1 specific amplification of Eucoleus böhmi.
Eucoleus böhmi (syn. Capillaria boehmi) is a trichuroid nematode affecting the epithelium of the nasal turbinates, frontal and paranasal sinuses of wild and domestic canids. Knowledge of the geographic distribution of nasal eucoleosis is fragmentary, despite the infection has been described from Europe and North America. Moreover, gaps exist in information available on the importance of the disease in canine clinical practice. The lack of knowledge on E. böhmi is likely due to limitations inherent to diagnostic methodologies. The aim of the present work was to assess a PCR-based assay instrumental to the amplification of a species-specific region of the mitochondrial DNA (mtDNA) gene encoding for the subunit 1 (cox1) gene of E. böhmi. Adult worms of E. böhmi from red foxes and dogs from Norway, Serbia and Italy and individual fecal samples from naturally infected dogs from Italy were included in the study. Stool samples from dogs negative for E. böhmi, but positive for other common parasites in both single and mixed infections, and adult stages of common dog parasites, were used to assess the specificity of this genetic assay. Using the panel of faecal samples, the assay showed a sensitivity of 85.14% and a specificity of 100%.
Faculty of Veterinary Medicine, University of Teramo, Piazza Aldo Moro 45, 64100 Teramo, Italy. Electronic address: angdicesare@gmail.com.
Angela
A
Di Cesare
Department of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06126 Perugia, Italy.
Fabrizia
F
Veronesi
Department of Animal Medicine, Production and Health, University of Padua, Viale dell'Università 16, 35020 Legnaro (PD), Italy.
Antonio
A
Frangipane di Regalbono
Istituto Zooprofilattico Sperimentale del Lazio e della Toscana "M. Aleandri", Via Appia Nuova 1411, 00178 Rome, Italy.
Claudio
C
De Liberato
Department of Veterinary Science, University of Pisa, Viale delle Piagge 2, 56124 Pisa,Italy.
Stefania
S
Perrucci
Faculty of Veterinary Medicine, University of Teramo, Piazza Aldo Moro 45, 64100 Teramo, Italy.
Raffaella
R
Iorio
Department of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06126 Perugia, Italy.
Giulia
G
Morganti
Department of Science of Agriculture, Food and Environment, University of Foggia, Via Napoli 25, 71121 Foggia, Italy.
Marianna
M
Marangi
Department of Animal Medicine, Production and Health, University of Padua, Viale dell'Università 16, 35020 Legnaro (PD), Italy.
Giulia
G
Simonato
Faculty of Veterinary Medicine, University of Teramo, Piazza Aldo Moro 45, 64100 Teramo, Italy.
Donato
D
Traversa
D000818:Animals; D001483:Base Sequence; D051545:Cyclooxygenase 1 / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics; D004283:Dog Diseases / Q000453:epidemiology* / Q000469:parasitology; D004285:Dogs; D017158:Enoplida / Q000235:genetics / Q000302:isolation & purification*; D017189:Enoplida Infections / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D005243:Feces / Q000469:parasitology; D005589:Foxes / Q000469:parasitology*; D007558:Italy / Q000453:epidemiology; D008928:Mitochondria / Q000201:enzymology; D008969:Molecular Sequence Data; D009664:Norway / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary; D055771:Serbia / Q000453:epidemiology
D004272:DNA, Mitochondrial; D051545:Cyclooxygenase 1
Diagnosis; Dog; Eucoleus böhmi; PCR; cox1