Combination of BMP-2-releasing gelatin/β-TCP sponges with autologous bone marrow for bone regeneration of X-ray-irradiated rabbit ulnar defects.
The objective of this study is to evaluate the feasibility of gelatin sponges incorporating β-tricalcium phosphate (β-TCP) granules (gelatin/β-TCP sponges) to enhance bone regeneration at a segmental ulnar defect of rabbits with X-ray irradiation. After X-ray irradiation of the ulnar bone, segmental critical-sized defects of 20-mm length were created, and bone morphogenetic protein-2 (BMP-2)-releasing gelatin/β-TCP sponges with or without autologous bone marrow were applied to the defects to evaluate bone regeneration. Both gelatin/β-TCP sponges containing autologous bone marrow and BMP-2-releasing sponges enhanced bone regeneration at the ulna defect to a significantly greater extent than the empty sponges (control). However, in the X-ray-irradiated bone, the bone regeneration either by autologous bone marrow or BMP-2 was inhibited. When combined with autologous bone marrow, the BMP-2 exhibited significantly high osteoinductivity, irrespective of the X-ray irradiation. The bone mineral content at the ulna defect was similar to that of the intact bone. It is concluded that the combination of bone marrow with the BMP-2-releasing gelatin/β-TCP sponge is a promising technique to induce bone regeneration at segmental bone defects after X-ray irradiation.
Department of Biomaterials, Field of Tissue Engineering, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan.
Masaya
M
Yamamoto
Department of Biomaterials, Field of Tissue Engineering, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan.
Akishige
A
Hokugo
Department of Biomaterials, Field of Tissue Engineering, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan.
Yoshitake
Y
Takahashi
Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University, Osaka, Japan.
Takayoshi
T
Nakano
Department of Therapeutic Radiology and Oncology, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Masahiro
M
Hiraoka
Department of Biomaterials, Field of Tissue Engineering, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan. Electronic address: yasuhiko@frontier.kyoto-u.ac.jp.
Yasuhiko
Y
Tabata
D000818:Animals; D015519:Bone Density; D001854:Bone Marrow Cells / Q000166:cytology*; D055396:Bone Morphogenetic Protein 2 / Q000008:administration & dosage*; D001861:Bone Regeneration / Q000187:drug effects*; D002130:Calcium Phosphates / Q000737:chemistry*; D004337:Drug Carriers; D016503:Drug Delivery Systems; D005780:Gelatin / Q000737:chemistry*; D008297:Male; D011817:Rabbits; D044968:Regenerative Medicine / Q000379:methods; D012867:Skin / Q000473:pathology; D013552:Swine; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds; D014457:Ulna / Q000473:pathology* / Q000528:radiation effects; D055114:X-Ray Microtomography; D014965:X-Rays
D055396:Bone Morphogenetic Protein 2; D002130:Calcium Phosphates; D004337:Drug Carriers; C485817:beta-tricalcium phosphate; D005780:Gelatin
Bone morphogenetic protein (BMP); Bone regeneration; Controlled release; Gelatin/β-tricalcium phosphate (TCP) sponges; Radiation; Segmental defect