Proteomics analysis of digestive juice from silkworm during Bombyx mori nucleopolyhedrovirus infection.
Previous studies have analyzed the midgut transcriptome and proteome after challenge with Bombyx mori nucleopolyhedrovirus (BmNPV), however little information is available on the digestive juice proteome after BmNPV challenge. This study investigated BmNPV infection-induced protein changes in the digestive juice of silkworms using shotgun proteomics and MS sequencing. From the digestive juice of normal third-day, fifth-instar silkworm larvae, 75 proteins were identified, 44 of which were unknown; from larvae 6 h after inoculation with BmNPV, 106 proteins were identified, of which 39 were unknown. After BmNPV challenge, more secreted proteins appeared that had antiviral and digestive features. GO annotation analysis clustered most proteins in the lumen into catalytic, binding, and metabolic processes. Numerous proteins were reported to have BmNPV interactions. Hsp70 protein cognate, lipase-1, and chlorophyllide A-binding protein precursor were upregulated significantly after BmNPV challenge. Levels of trypsin-like serine protease, beta-1,3-glucanase, catalase, and serine protease transcripts decreased or were not significantly change after BmNPV challenge. Taken together, these findings provided insights into the interaction between host and BmNPV and revealed potential functions of digestive juice after per os BmNPV infection.
School of Biology and Basic Medical Science, Soochow University, Suzhou, P. R. China.|National Engineering Laboratory for Modern Silk, Soochow University, Suzhou, P. R. China.
Xiaolong
X
Hu
School of Biology and Basic Medical Science, Soochow University, Suzhou, P. R. China.
Min
M
Zhu
School of Biology and Basic Medical Science, Soochow University, Suzhou, P. R. China.
Simei
S
Wang
School of Biology and Basic Medical Science, Soochow University, Suzhou, P. R. China.
Liyuan
L
Zhu
School of Biology and Basic Medical Science, Soochow University, Suzhou, P. R. China.|National Engineering Laboratory for Modern Silk, Soochow University, Suzhou, P. R. China.
Renyu
R
Xue
School of Biology and Basic Medical Science, Soochow University, Suzhou, P. R. China.|National Engineering Laboratory for Modern Silk, Soochow University, Suzhou, P. R. China.
Guangli
G
Cao
School of Biology and Basic Medical Science, Soochow University, Suzhou, P. R. China.|National Engineering Laboratory for Modern Silk, Soochow University, Suzhou, P. R. China.
Chengliang
C
Gong
D000818:Animals; D012831:Bombyx / Q000378:metabolism* / Q000821:virology; D002851:Chromatography, High Pressure Liquid; D004064:Digestive System / Q000378:metabolism* / Q000821:virology; D004591:Electrophoresis, Polyacrylamide Gel; D015971:Gene Expression Regulation, Enzymologic; D063990:Gene Ontology; D054884:Host-Pathogen Interactions; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D007814:Larva / Q000378:metabolism / Q000821:virology; D058977:Molecular Sequence Annotation; D017924:Nucleopolyhedroviruses / Q000254:growth & development* / Q000502:physiology; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D021241:Spectrometry, Mass, Electrospray Ionization
D019476:Insect Proteins; D020543:Proteome
Animal proteomics; Antivirus; Bombyx mori; Bombyx mori nucleopolyhedrovirus; Digestive juice; Midgut