Cell shape change and invagination of the cephalic furrow involves reorganization of F-actin.
Invagination of epithelial sheets to form furrows is a fundamental morphogenetic movement and is found in a variety of developmental events including gastrulation and vertebrate neural tube formation. The cephalic furrow is a deep epithelial invagination that forms during Drosophila gastrulation. In the first phase of cephalic furrow formation, the initiator cells that will lead invagination undergo apicobasal shortening and apical constriction in the absence of epithelial invagination. In the second phase of cephalic furrow formation, the epithelium starts to invaginate, accompanied by both basal expansion and continued apicobasal shortening of the initiator cells. The cells adjacent to the initiator cells also adopt wedge shapes, but only after invagination is well underway. Myosin II does not appear to drive apical constriction in cephalic furrow formation. However, cortical F-actin is increased in the apices of the initiator cells and in invaginating cells during both phases of cephalic furrow formation. These findings suggest that a novel mechanism for epithelial invagination is involved in cephalic furrow formation.
Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, 3601 4th Street, STOP 6540, Lubbock, TX 79430, United States.
Allison K
AK
Spencer
Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, 3601 4th Street, STOP 6540, Lubbock, TX 79430, United States.
Bilal A
BA
Siddiqui
Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, 3601 4th Street, STOP 6540, Lubbock, TX 79430, United States. Electronic address: jeffrey.thomas@ttuhsc.edu.
Jeffrey H
JH
Thomas
D000199:Actins / Q000378:metabolism*; D022005:Adherens Junctions / Q000502:physiology; D000818:Animals; D001921:Brain / Q000196:embryology* / Q000648:ultrastructure; D048430:Cell Shape / Q000502:physiology*; D004330:Drosophila / Q000196:embryology*; D004848:Epithelium / Q000196:embryology*; D054262:Gastrulation / Q000502:physiology*; D007091:Image Processing, Computer-Assisted; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D009024:Morphogenesis / Q000502:physiology*; D059008:Time-Lapse Imaging
D000199:Actins
Apical constriction; Cell shape change; Cephalic furrow; Gastrulation; Invagination; Morphogenesis