Lymphocyte subset reference intervals in blood donors from northeastern Brazil.
The reference intervals for leukocytes and lymphocytes currently used by most clinical laboratories present limitations as they are primarily derived from individuals of North American and European origin. The objective this study was to determine reference values for peripheral blood B lymphocytes, T lymphocyte subsets (CD4+, CD8+, naïve, memory, regulatory, TCRαβ and TCRγδ+) and NK cells from blood donors in Salvador-Bahia, Brazil.


RESULTS
The proportion of included male subjects was 73.7% and the median ages of males (34) and females (35) were found to be similar. Absolute counts total lymphocytes subsets to both gender was 1,956 (1,060-4,186) cells and relative values 34%. The T CD4+ and T CD8+ lymphocytes relative values was 51% (20-62) and 24% (9-28), respectively. The most statistically significant finding observed was a higher percentage of B lymphocytes (p=0.03) in females. Commonly cited subset reference intervals were found to be consistent with values in several populations from different geographic areas.
Hospital Universitário Professor Edgard Santos, Universidade Federal da Bahia, Salvador, BA, Brasil.
Alex J L
AJ
Torres
Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, BA, Brasil.
Patrícia
P
Cisneiros
Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, BA, Brasil.
Rosa
R
Guedes
Escola Bahiana de Medicina e Saúde Pública, Salvador, BA, Brasil.
Maria Fernanda R
MF
Grassi
Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, BA, Brasil.
Roberto
R
Meyer
Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, BA, Brasil.
Maria T
MT
Bendicho
Hospital Universitário Professor Edgard Santos, Universidade Federal da Bahia, Salvador, BA, Brasil.
Tais G S L
TG
Lopes
Hospital Universitário Professor Edgard Santos, Universidade Federal da Bahia, Salvador, BA, Brasil.
Gabriela
G
Félix
Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, BA, Brasil.
Eduardo M
EM
Netto
Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, BA, Brasil.
Carlos
C
Brites
Hospital Universitário Professor Edgard Santos, Universidade Federal da Bahia, Salvador, BA, Brasil.
Kiyoko
K
Abe-Sandes
Fundação de Hematologia e Hemoterapia da Bahia, Salvador, BA, Brasil.
Claudio
C
Brandão
Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, BA, Brasil.
Neuza
N
Alcantara-Neves
Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, BA, Brasil.
Songeli M
SM
Freire
D000293:Adolescent; D000328:Adult; D001402:B-Lymphocytes / Q000166:cytology*; D001782:Blood Donors; D001938:Brazil; D003430:Cross-Sectional Studies; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007694:Killer Cells, Natural / Q000166:cytology*; D018655:Lymphocyte Count; D016131:Lymphocyte Subsets / Q000166:cytology*; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D055815:Young Adult