Croton argenteus preparation inhibits initial growth, mitochondrial respiration and increase the oxidative stress from Senna occidentalis seedlings.
Senna ocidentalis is a weed, native to Brazil, considered to infest crops and plantations, and is responsible for yield losses of several crops, particularly soybean. The aim of this work was to evaluate if the Croton argenteus extract and fractions possess phytotoxic activity on S. ocidentalis. The crude ethanolic extract (CEE) and its hexanic (HF), chloroformic (CLF) and ethyl acetate (EAF) fractions were tested in germination, growth, oxidative stress increase, Adenosine triphosphate, L-malate and succinate synthesis. The crude extract and its fractions slowed down the germination of S. ocidentalis and decreased the final percentage of germination. Oxidative stress was also increased in the seedlings, by an increase of catalase, peroxidase, superoxide dismutase, glutathione reductase and lipid peroxidation; and it became clear that the ethyl acetate fraction was more phytotoxic. The results indicate that the crude extract and fractions of C. argenteus compromise the mitochondrial energy metabolism, by the inhibition of mitochondrial ATP production, with a decrease in the production of L-malate and succinate. The ethyl acetate fraction of C. argenteus showed high activity on germination and growth, and these effects take place by means of mitochondrial metabolism alterations and increase the oxidative stress, leading the seedling death.
Departamento de Farmácia, Universidade Federal do Paraná, Curitiba, PR, Brasil.
Katlin S
KS
Rech
Departamento de Farmácia, Universidade Federal do Paraná, Curitiba, PR, Brasil.
Cristiane B
CB
Silva
Departamento de Farmácia, Universidade Federal do Paraná, Curitiba, PR, Brasil.
Juliana D
JD
Kulik
Departamento de Farmácia, Universidade Federal do Paraná, Curitiba, PR, Brasil.
Josiane F G
JF
Dias
Departamento de Farmácia, Universidade Federal do Paraná, Curitiba, PR, Brasil.
Sandra M W
SM
Zanin
Departamento de Farmácia, Universidade Federal do Paraná, Curitiba, PR, Brasil.
Vitor A
VA
Kerber
Departamento de Farmácia, Universidade Federal do Paraná, Curitiba, PR, Brasil.
Fernanda M M
FM
Ocampos
Departamento de Farmácia, Universidade Federal do Paraná, Curitiba, PR, Brasil.
Luciane
L
Dalarmi
Departamento de Biologia, Universidade Federal do Paraná, Curitiba, PR, Brasil.
Gedir O
GO
Santos
Departamento de Química, Universidade Estadual de Mato Grosso do Sul, Naviraí, MS, Brasil.
Euclésio
E
Simionatto
Departamento de Farmácia, Universidade Federal do Paraná, Curitiba, PR, Brasil.
Cristina P
CP
Lima
Departamento de Farmácia, Universidade Federal do Paraná, Curitiba, PR, Brasil.
Obdúlio G
OG
Miguel
Departamento de Farmácia, Universidade Federal do Paraná, Curitiba, PR, Brasil.
Marilis D
MD
Miguel
D029802:Croton / Q000737:chemistry* / Q000145:classification; D018525:Germination / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects* / Q000502:physiology; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D036226:Seedlings / Q000187:drug effects / Q000254:growth & development; D029846:Senna Plant / Q000187:drug effects* / Q000254:growth & development
D010936:Plant Extracts