Differential expression of miRNAs and their relation to active tuberculosis.
The aim of this work was to screen miRNA signatures dysregulated in tuberculosis to improve our understanding of the biological role of miRNAs involved in the disease. Datasets deposited in publically available databases from microarray studies on infectious diseases and malignancies were retrieved, screened, and subjected to further analysis. Effect sizes were combined using the inverse-variance model and between-study heterogeneity was evaluated by the random effects model. 35 miRNAs were differentially expressed (12 up-regulated, 23 down-regulated; p < 0.05) by combining 15 datasets of tuberculosis and other infectious diseases. 15 miRNAs were found to be significantly differentially regulated (7 up-regulated, 8 down-regulated; p < 0.05) by combining 53 datasets of tuberculosis and malignancies. Most of the miRNA signatures identified in this study were found to be involved in immune responses and metabolism. Expression of these miRNA signatures in serum samples from TB subjects (n = 11) as well as healthy controls (n = 10) was examined by TaqMan miRNA array. Taken together, the results revealed differential expression of miRNAs in TB, but available datasets are limited and these miRNA signatures should be validated in future studies.
Laboratory of Bacterial Pathogenesis, Department of Microbiology and Immunology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
Zhihong
Z
Xu
Laboratory of Bacterial Pathogenesis, Department of Microbiology and Immunology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; Department of Laboratory Medicine, East Hospital Affiliated to Tongji University, Shanghai 200120, China.
Aiping
A
Zhou
Laboratory of Bacterial Pathogenesis, Department of Microbiology and Immunology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
Jinjing
J
Ni
Laboratory of Bacterial Pathogenesis, Department of Microbiology and Immunology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
Qiufen
Q
Zhang
Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
Ying
Y
Wang
Shanghai Ruijin Hospital, Shanghai 200025, China.
Jie
J
Lu
Department of Laboratory Medicine, East Hospital Affiliated to Tongji University, Shanghai 200120, China.
Wenjuan
W
Wu
Center for Tuberculosis Research, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
Petros C
PC
Karakousis
Shanghai Public Health Clinical Center, Shanghai 201508, China. Electronic address: tubercle@shaphc.org.
Shuihua
S
Lu
Laboratory of Bacterial Pathogenesis, Department of Microbiology and Immunology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; Department of Laboratory Medicine, East Hospital Affiliated to Tongji University, Shanghai 200120, China. Electronic address: yfyao@sjtu.edu.cn.
Yufeng
Y
Yao
D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D019295:Computational Biology; D030541:Databases, Genetic; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D005819:Genetic Markers; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D014376:Tuberculosis / Q000097:blood / Q000175:diagnosis / Q000235:genetics* / Q000382:microbiology; D055815:Young Adult
D005819:Genetic Markers; D035683:MicroRNAs
Microarray; Tuberculosis; microRNA