Comparison of the Diversity of Basidiomycetes from Dead Wood of the Manchurian fir (Abies holophylla) as Evaluated by Fruiting Body Collection, Mycelial Isolation, and 454 Sequencing.
In this study, three different methods (fruiting body collection, mycelial isolation, and 454 sequencing) were implemented to determine the diversity of wood-inhabiting basidiomycetes from dead Manchurian fir (Abies holophylla). The three methods recovered similar species richness (26 species from fruiting bodies, 32 species from mycelia, and 32 species from 454 sequencing), but Fisher's alpha, Shannon-Wiener, Simpson's diversity indices of fungal communities indicated fruiting body collection and mycelial isolation displayed higher diversity compared with 454 sequencing. In total, 75 wood-inhabiting basidiomycetes were detected. The most frequently observed species were Heterobasidion orientale (fruiting body collection), Bjerkandera adusta (mycelial isolation), and Trichaptum fusco-violaceum (454 sequencing). Only two species, Hymenochaete yasudae and Hypochnicium karstenii, were detected by all three methods. This result indicated that Manchurian fir harbors a diverse basidiomycetous fungal community and for complete estimation of fungal diversity, multiple methods should be used. Further studies are required to understand their ecology in the context of forest ecosystems.
School of Biological Sciences, Seoul National University, Seoul, 151-747, Republic of Korea.
Yeongseon
Y
Jang
Division of Environmental Science and Ecological Engineering, College of Life Sciences and Biotechnology, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, 136-701, Korea.
Seokyoon
S
Jang
Division of Environmental Science and Ecological Engineering, College of Life Sciences and Biotechnology, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, 136-701, Korea.
Mihee
M
Min
Division of Environmental Science and Ecological Engineering, College of Life Sciences and Biotechnology, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, 136-701, Korea.
Joo-Hyun
JH
Hong
Division of Environmental Science and Ecological Engineering, College of Life Sciences and Biotechnology, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, 136-701, Korea.
Hanbyul
H
Lee
Division of Environmental Science and Ecological Engineering, College of Life Sciences and Biotechnology, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, 136-701, Korea.
Hwanhwi
H
Lee
School of Biological Sciences, Seoul National University, Seoul, 151-747, Republic of Korea.
Young Woon
YW
Lim
Division of Environmental Science and Ecological Engineering, College of Life Sciences and Biotechnology, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, 136-701, Korea. jae-jinkim@korea.ac.kr.
Jae-Jin
JJ
Kim
D028202:Abies / Q000382:microbiology*; D001487:Basidiomycota / Q000502:physiology*; D044822:Biodiversity; D004271:DNA, Fungal / Q000235:genetics; D021901:DNA, Intergenic / Q000235:genetics; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D008969:Molecular Sequence Data; D025282:Mycelium / Q000737:chemistry; D009172:Mycology / Q000379:methods*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D014934:Wood / Q000382:microbiology*
D004271:DNA, Fungal; D021901:DNA, Intergenic
Dead wood; Mycoparasitic fungi; Mycorrhizal fungi; Wood-decay fungi