2015 Guidelines for Establishing Genetically Modified Rat Models for Cardiovascular Research.
The rat has long been a key physiological model for cardiovascular research, most of the inbred strains having been previously selected for susceptibility or resistance to various cardiovascular diseases (CVD). These CVD rat models offer a physiologically relevant background on which candidates of human CVD can be tested in a more clinically translatable experimental setting. However, a diverse toolbox for genetically modifying the rat genome to test molecular mechanisms has only recently become available. Here, we provide a high-level description of several strategies for developing genetically modified rat models of CVD.
Human and Molecular Genetics Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, 53226, WI, USA, mflister@mcw.edu.
Michael J
MJ
Flister
Jeremy W
JW
Prokop
Jozef
J
Lazar
Mary
M
Shimoyama
Melinda
M
Dwinell
Aron
A
Geurts
D000818:Animals; D035843:Biomedical Research / Q000592:standards*; D002318:Cardiovascular Diseases / Q000175:diagnosis / Q000235:genetics* / Q000503:physiopathology / Q000628:therapy; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D005821:Genetic Techniques / Q000592:standards*; D006801:Humans; D010641:Phenotype; D051381:Rats; D055647:Rats, Transgenic; D013045:Species Specificity
D005819:Genetic Markers