Raman spectroscopic study on the excystation process in a single unicellular organism amoeba (Acanthamoeba polyphaga).
An in vivo Raman spectroscopic study of amoeba (Acanthamoeba polyphaga) is presented. The changes of the spectra during the amoeba cyst activation and excystation are analyzed. The spectra show the changes of the relative intensities of bands corresponding to protein, lipid, and carotenoid components during cyst activation. The presence of carotenoids in the amoeba is observed via characteristic Raman bands. These signals in the Raman spectra are intense in cysts but decrease in intensity with cyst activation and exhibit a correlation with the life cycle of amoeba. This work demonstrates the feasibility of using Raman spectroscopy for the detection of single amoeba microorganisms in vivo and for the analysis of the amoeba life activity. The information obtained may have implications for the estimation of epidemiological situations and for the diagnostics and prognosis of the development of amoebic inflammations.
National Dong Hwa University, Department of Physics, 1, Sec. 2, Da-Hsueh Road, Shoufeng, Hualien 97401, Taiwan.
Yu-Chung
YC
Lin
National Dong Hwa University, Department of Physics, 1, Sec. 2, Da-Hsueh Road, Shoufeng, Hualien 97401, TaiwanbP.N. Lebedev Physics Institute, Russian Academy of Science, Moscow 119991, Russia.
Elena
E
Perevedentseva
National Dong Hwa University, Department of Physics, 1, Sec. 2, Da-Hsueh Road, Shoufeng, Hualien 97401, Taiwan.
Chia-Liang
CL
Cheng
D000048:Acanthamoeba / Q000166:cytology* / Q000502:physiology*; D002338:Carotenoids / Q000378:metabolism*; D008018:Life Cycle Stages / Q000502:physiology*; D050356:Lipid Metabolism / Q000502:physiology*; D057054:Molecular Imaging / Q000379:methods; D015800:Protozoan Proteins / Q000378:metabolism*; D013059:Spectrum Analysis, Raman / Q000379:methods
D015800:Protozoan Proteins; D002338:Carotenoids