[Lentivirus-mediated shRNA silencing of LAMP2A inhibits the proliferation of multiple myeloma cells].
OBJECTIVE
To study the effects of lentivirus-mediated short hairpin RNA (shRNA) silencing of lysosome-associated membrane protein type 2A (LAMP2A) expression on the proliferation of multiple myeloma cells.


METHODS
The constructed shRNA lentiviral vector was applied to infect human multiple myeloma cell line MM.1S, and stable expression cell line was obtained by puromycin screening. Western blotting was used to verify the inhibitory effect on LAMP2A protein expression. MTT assay was conducted to detect the effect of knocked-down LAMP2A on MM.1S cell proliferation, and the anti-tumor potency of suberoylanilide hydroxamic acid (SAHA) against the obtained MM.1S LAMP2A(shRNA) stable cell line. Lactate assay was performed to observe the impact of low LAMP2A expression on cell glycolysis.


RESULTS
The stable cell line with low LAMP2A expression were obtained with the constructed human LAMP2A-shRNA lentiviral vector. Down-regulation of LAMP2A expression significantly inhibited MM.1S cell proliferation and enhanced the anti-tumor activity of SAHA. Interestingly, decreased LAMP2A expression also inhibited MM.1S cell lactic acid secretion.


CONCLUSIONS
Down-regulation of LAMP2A expression could inhibit cell proliferation in multiple myeloma cells.
Interdisciplinary Higher Institute of Science and Technology, East China Normal University, Shanghai 200062; State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, SIBS, Chinese Academy of Sciences, Shanghai 201203, China.
Lixuan
L
Li
Interdisciplinary Higher Institute of Science and Technology, East China Normal University, Shanghai 200062; State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, SIBS, Chinese Academy of Sciences, Shanghai 201203, China.
Jia
J
Li
D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D015536:Down-Regulation / Q000235:genetics; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism; D016086:Lentivirus / Q000235:genetics*; D052119:Lysosomal-Associated Membrane Protein 2 / Q000235:genetics*; D009101:Multiple Myeloma / Q000473:pathology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*
D052119:Lysosomal-Associated Membrane Protein 2; D034741:RNA, Small Interfering; D019344:Lactic Acid