Neuronal tumour necrosis factor-α and interleukin-1β expression in a porcine model of intracerebral haemorrhage: Modulation by U-74389G.
Tumour necrosis factor α (TNF-α) and interleukin 1β (IL-1β) are important mediators of intracerebral haemorrhage (ICH) inflammatory response. Lazaroids, established antioxidants and neuroprotectants, have been studied in several brain pathologies. The present study was designed to investigate: a) TNF-α and IL-1β changes, in neurons and b) U-74389G effects, 4 and 24h after haematoma induction in a porcine model of intracerebral haemorrhage. In twenty male landrace pigs (swines) aged 135-150 days old, autologous whole blood was injected around the right basal ganglia territory; in ten of the pigs the lazaroid compound U-74389G was administered. Brain TNF-α and IL-1β immunopositive neurons were determined by immunoarray techniques at 4 and 24h timepoints. After the haematoma induction the number of TNF-α immunopositive neurons ipsilateral to the haematoma was significantly higher compared to the contralateral site at 4h (p<0.0005), while U-74389G significantly reduced the number of TNF-α immunopositive neurons, ipsilateral to the haematoma, at 4h (p=0.002); at 24h, TNF-α immunopositive neurons were found significantly lower in the control group ipsilateral to the haematoma in comparison to 4h timepoint(p<0.0005). The number of IL-1β immunopositive neurons at 4h after the hematoma induction was significantly higher ipsilateral to the haematoma site (p<0.0005). U-74389G had no statistical significant effect. TNF-α and IL-1β, increase in neurons, 4h after the haematoma induction, ipsilateral to the haematoma site. The administration of the antioxidant compound U-74389G, results in early (at 4h) decrease of TNF-α immunopositive neurons but shows no statistical significant effect to IL-1β immunopossitive neurons.
Laboratory of Pharmacology, National and Kapodistrian University of Athens, 75 Mikras Asias str, 11527 Athens, Greece.
Alexios
A
Bimpis
Experimental-Research Center ELPEN, Athens, Greece.
Apostolos
A
Papalois
2nd Department of Neurology, Medical School, National and Kapodistrian University of Athens, Greece.
Konstantinos
K
Voumvourakis
Department of Physiology, Faculty of Medicine, University of Szeged, Hungary.
Orsolya
O
Oláh
Department of Pathology, Medical Faculty of University of Szeged, Hungary.
Lazlo
L
Tiszlavicz
Laboratory of Pharmacology, National and Kapodistrian University of Athens, 75 Mikras Asias str, 11527 Athens, Greece. Electronic address: cliapi@med.uoa.gr.
Charis
C
Liapi
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D001479:Basal Ganglia / Q000187:drug effects / Q000378:metabolism; D002543:Cerebral Hemorrhage / Q000378:metabolism* / Q000517:prevention & control; D053583:Interleukin-1beta / Q000378:metabolism*; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D011281:Pregnatrienes / Q000008:administration & dosage*; D013552:Swine; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D000975:Antioxidants; D053583:Interleukin-1beta; D011281:Pregnatrienes; D014409:Tumor Necrosis Factor-alpha; C074965:U 74389F
IL-1β; Intracerebral haemorrhage; Porcine model; TNF-α; U-74389G