Effects of Combined Soy Isoflavone Extract and Docetaxel Treatment on Murine 4T1 Breast Tumor Model.
BACKGROUND
Emergence of drug resistance has brought major problems in chemotherapy. Using nutrients in combination with chemotherapy could be beneficial for improvement of sensitivity of tumors to drug resistance. Soybean-derived isoflavones have been suggested as chemopreventive agents for certain types of cancer, particularly breast cancer. In this study, the synergistic effects of soy isoflavone extract in combination with docetaxel in murine 4T1 breast tumor model were investigated.


METHODS
In this study, mice were divided into 4 groups (15 mice per group) of control, the dietary Soy Isoflavone Extract (SIE, 100 mg/kg diet), the Docetaxel (DOCE, 10 mg/kg) injection and the combination of dietary soy isoflavone extract and intravenous docetaxel injection (DOCE+SIE). After 3 injections of docetaxel (once a week), 7 mice were sacrificed to analyze MKI67 gene and protein expressions and the rest were monitored for diet consumption, tumor growth and survival rates.


RESULTS
In DOCE+SIE group, diet consumption was significantly higher than DOCE group. While lifespan showed a trend towards improvement in DOCE+SIE group, no significant difference was observed among the 4 studied groups. Tumor volume was not significantly affected in treated groups. A lower but not significant MKI67 protein expression was detected in western blot in DOCE+SIE group. The mRNA expression was not significantly different among groups.


CONCLUSIONS
The results suggest that the combination of soy isoflavone as an adjunct to docetaxel chemotherapy can be effective in improving diet consumption in breast cancer.
Department of Clinical Nutrition and Dietetics, School of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Ehsan
E
Hejazi
Department of Clinical Nutrition and Dietetics, School of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Javad
J
Nasrollahzadeh
Reproductive Immunology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
Ramina
R
Fatemi
Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
Leila
L
Barzegar-Yar Mohamadi
Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
Kioomars
K
Saliminejad
Department of Basic Sciences and Cellular and Molecular Nutrition, School of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Zohre
Z
Amiri
Department of Clinical Nutrition and Dietetics, School of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Masoud
M
Kimiagar
Department of Radiation Oncology, Shohada Tajrish Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Mohammad
M
Houshyari
Reproductive Immunology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
Maryam
M
Tavakoli
Reproductive Immunology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
Farah
F
Idali
Breast cancer; Docetaxel; Soy isoflavone extract