Challenges of biomolecular detection at the nanoscale: nanopores and microelectrodes.
The interest in analytical devices, which typically rely on the reactivity of a biological component for specificity, is growing rapidly. In this Perspective, we highlight current challenges in all-electrical biosensing as these systems shrink toward the nanoscale and enable the detection of analytes at the single-molecule level. We focus on two sensing principles: nanopores and amperometric microelectrode devices.
†Pharmaceutical Analysis, Groningen Research Institute of Pharmacy, University of Groningen, P.O. Box 196, 9700 AD Groningen, The Netherlands.
Klaus
K
Mathwig
‡Department of Chemistry, Imperial College London, Exhibition Road, South Kensington Campus, London, SW7 2AZ, U.K.
Tim
T
Albrecht
§Department of Chemical Engineering, Northeastern University, 360 Huntington Avenue, 313SN, Boston, Massachusetts 02115, United States.
Edgar D
ED
Goluch
¶Department of Chemical Engineering, Delft University of Technology, Julianalaan 136, 2628 BL Delft, The Netherlands.
Liza
L
Rassaei
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004247:DNA / Q000032:analysis*; D008839:Microelectrodes; D058608:Nanopores; D011506:Proteins / Q000032:analysis*
D011506:Proteins; D004247:DNA