Proteomic analysis of the European flounder Platichthys flesus response to experimental PAH-PCB contamination.
Platichthys flesus is often used as a sentinel species to monitor the estuarine water quality. In this study, we carried out an experimental contamination of fish using a PAHs/PCBs mixture, which was designed to mimic the concentrations found in the Seine estuary (C1) and 10 times these concentrations (C2). We used a proteomic approach to understand the molecular mechanisms implied in the response of P. flesus to these xenobiotics. We showed that 54 proteins were differentially accumulated in one or several conditions, which 34 displayed accumulation factors higher than two. 18 of these proteins were identified by MALDI TOF-TOF mass spectrometry. The results indicated the deregulation of oxidative stress- and glutathione metabolism-(GST, GPx) proteins as well as of several proteins belonging to the betaine demethylation pathway and the methionine cycle (BHMT, SHMT, SAHH), suggesting a role for these different pathways in the P. flesus response to chemical contamination.
Université de Brest, Laboratoire des Sciences de l'Environnement Marin, LEMAR, UMR 6539 UBO/CNRS/IRD/Ifremer, Institut Universitaire Européen de la Mer (IUEM), 29280 Plouzané, France.
Claire
C
Galland
Université de Brest, Laboratoire des Sciences de l'Environnement Marin, LEMAR, UMR 6539 UBO/CNRS/IRD/Ifremer, Institut Universitaire Européen de la Mer (IUEM), 29280 Plouzané, France.
Célie
C
Dupuy
Unité de Biogéochimie et Ecotoxicologie, IFREMER, Centre de Brest, BP70, 29280 Plouzané, France.
Véronique
V
Loizeau
ANSES, Agence nationale de sécurité sanitaire de l'alimentation et de l'environnement et du travail, site de Ploufragan-Plouzané-Technopole Brest Iroise, 29280 Plouzané, France.
Morgane
M
Danion
Université de Brest, Laboratoire des Sciences de l'Environnement Marin, LEMAR, UMR 6539 UBO/CNRS/IRD/Ifremer, Institut Universitaire Européen de la Mer (IUEM), 29280 Plouzané, France.
Michel
M
Auffret
Université de Brest, Laboratoire des Sciences de l'Environnement Marin, LEMAR, UMR 6539 UBO/CNRS/IRD/Ifremer, Institut Universitaire Européen de la Mer (IUEM), 29280 Plouzané, France.
Louis
L
Quiniou
Université de Brest, Laboratoire des Sciences de l'Environnement Marin, LEMAR, UMR 6539 UBO/CNRS/IRD/Ifremer, Institut Universitaire Européen de la Mer (IUEM), 29280 Plouzané, France.
Jean
J
Laroche
Université de Brest, Laboratoire des Sciences de l'Environnement Marin, LEMAR, UMR 6539 UBO/CNRS/IRD/Ifremer, Institut Universitaire Européen de la Mer (IUEM), 29280 Plouzané, France. Electronic address: vianney.pichereau@univ-brest.fr.
Vianney
V
Pichereau
D000818:Animals; D004784:Environmental Monitoring; D063366:Estuaries; D005432:Flounder / Q000378:metabolism / Q000502:physiology*; D008099:Liver / Q000378:metabolism; D011078:Polychlorinated Biphenyls / Q000032:analysis / Q000633:toxicity*; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000633:toxicity*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*; D015262:Xenobiotics
D011084:Polycyclic Aromatic Hydrocarbons; D020543:Proteome; D014874:Water Pollutants, Chemical; D015262:Xenobiotics; D011078:Polychlorinated Biphenyls
Betaine and methionine metabolism; Biomarker; Persistent organic pollutants; Platichthys flesus; Pollution; Proteomics