Tong Xie Yao Fang relieves irritable bowel syndrome in rats via mechanisms involving regulation of 5-hydroxytryptamine and substance P.
OBJECTIVE
To investigate whether the Chinese medicine Tong Xie Yao Fang (TXYF) improves dysfunction in an irritable bowel syndrome (IBS) rat model.


METHODS
Thirty baby rats for IBS modeling were separated from mother rats (1 h per day) from days 8 to 21, and the rectum was expanded by angioplasty from days 8 to 12. Ten normal rats were used as normal controls. We examined the effects of TXYF on defection frequency, colonic transit function and smooth muscle contraction, and the expression of 5-hydroxytryptamine (5-HT) and substance P (SP) in colonic and hypothalamus tissues by Western blot and RT-PCT techniques in both normal rats and IBS model rats with characterized visceral hypersensitivity.


RESULTS
Defecation frequency was 1.8 ± 1.03 in normal rats and 4.5 ± 1.58 in IBS model rats (P < 0.001). However, the defecation frequency was significantly decreased (3.0 ± 1.25 vs 4.5 ± 1.58, P < 0.05), while the time (in seconds) of colon transit function was significantly increased (256.88 ± 20.32 vs 93.36 ± 17.28, P < 0.001) in IBS + TXYF group rats than in IBS group rats. Increased colonic smooth muscle tension and contract frequency in IBS model rats were significantly decreased by administration of TXYF. Exogenous agonist stimulants increased spontaneous activity and elicited contractions of colon smooth muscle in IBS model rats, and all of these actions were significantly reduced by TXYF involving 5-HT and SP down-regulation.


CONCLUSIONS
TXYF can modulate the activity of the enteric nervous system and alter 5-HT and SP activities, which may contribute to the symptoms of IBS.
Yue Yin, Jian-Wei Wang, Xue-Ying Zhao, Wen-Jing Zhao, Chinese Medicine Teaching and Research Office, Basic Medical College of Heilongjiang University of Chinese Medicine, Harbin 150040, Heilongjiang Province, China.
Yue
Y
Yin
Yue Yin, Jian-Wei Wang, Xue-Ying Zhao, Wen-Jing Zhao, Chinese Medicine Teaching and Research Office, Basic Medical College of Heilongjiang University of Chinese Medicine, Harbin 150040, Heilongjiang Province, China.
Lei
L
Zhong
Yue Yin, Jian-Wei Wang, Xue-Ying Zhao, Wen-Jing Zhao, Chinese Medicine Teaching and Research Office, Basic Medical College of Heilongjiang University of Chinese Medicine, Harbin 150040, Heilongjiang Province, China.
Jian-Wei
JW
Wang
Yue Yin, Jian-Wei Wang, Xue-Ying Zhao, Wen-Jing Zhao, Chinese Medicine Teaching and Research Office, Basic Medical College of Heilongjiang University of Chinese Medicine, Harbin 150040, Heilongjiang Province, China.
Xue-Ying
XY
Zhao
Yue Yin, Jian-Wei Wang, Xue-Ying Zhao, Wen-Jing Zhao, Chinese Medicine Teaching and Research Office, Basic Medical College of Heilongjiang University of Chinese Medicine, Harbin 150040, Heilongjiang Province, China.
Wen-Jing
WJ
Zhao
Yue Yin, Jian-Wei Wang, Xue-Ying Zhao, Wen-Jing Zhao, Chinese Medicine Teaching and Research Office, Basic Medical College of Heilongjiang University of Chinese Medicine, Harbin 150040, Heilongjiang Province, China.
Hai-Xue
HX
Kuang
D000818:Animals; D000831:Animals, Newborn; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D003106:Colon / Q000187:drug effects* / Q000294:innervation / Q000378:metabolism / Q000503:physiopathology; D003672:Defecation / Q000187:drug effects; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D017615:Enteric Nervous System / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D005765:Gastrointestinal Agents / Q000494:pharmacology*; D005769:Gastrointestinal Motility / Q000187:drug effects; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D043183:Irritable Bowel Syndrome / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D017208:Rats, Wistar; D012701:Serotonin / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D013373:Substance P / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors
D000893:Anti-Inflammatory Agents; D004365:Drugs, Chinese Herbal; D005765:Gastrointestinal Agents; C000601593:tongxie-yaofang; D012701:Serotonin; D013373:Substance P
5-hydroxytryptamine; Irritable bowel syndrome; Substance P; Tong Xie Yao Fang; Traditional Chinese medicine