Accumulation of GD1α Ganglioside in MDA-MB-231 Breast Cancer Cells Expressing ST6GalNAc V.
α-Series gangliosides define a particular sub-class of glycosphingolipids containing sialic acid α2,6-linked to GalNAc residue that was isolated as a minor compound from the brain. The sialyltransferase ST6GalNAc V was cloned from mouse brain and showed α2,6-sialyltransferase activity almost exclusively for GM1b, to form GD1α and is considered as the main enzyme involved in the biosynthesis of α-series gangliosides. Recently, ST6GALNAC5 was identified as one of the genes over-expressed in breast cancer cell populations selected for their ability to produce brain metastasis. However, the capacity of human breast cancer cells to produce α-series gangliosides has never been clearly demonstrated. Here, we show by stable transfection and MS-MS analysis of total glycosphingolipids that ST6GALNAC5 expressing MDA-MB-231 breast cancer cells accumulate GD1α ganglioside (IV3Neu5Ac1, III6Neu5Ac1Gg4-Cer).
Structural and Functional Glycobiology Unit, UMR CNRS 8576, University of Lille, 59655 Villeneuve d'Ascq, France. sandy.vandermeersch@gmail.com.
Sandy
S
Vandermeersch
Structural and Functional Glycobiology Unit, UMR CNRS 8576, University of Lille, 59655 Villeneuve d'Ascq, France. jorick.vanbeselaere@gmail.com.
Jorick
J
Vanbeselaere
Structural and Functional Glycobiology Unit, UMR CNRS 8576, University of Lille, 59655 Villeneuve d'Ascq, France. cl-ment.delannoy@etudiant.univ-lille1.fr.
Clément P
CP
Delannoy
Blood Brain Barrier Laboratory (LBHE), EA 2465, University of Artois, 62300 Lens, France. aurore.drolez@univ-artois.fr.
Aurore
A
Drolez
Blood Brain Barrier Laboratory (LBHE), EA 2465, University of Artois, 62300 Lens, France. cj.mysiorek@gmail.com.
Caroline
C
Mysiorek
Structural and Functional Glycobiology Unit, UMR CNRS 8576, University of Lille, 59655 Villeneuve d'Ascq, France. yann.guerardel@univ-lille1.fr.
Yann
Y
Guérardel
Structural and Functional Glycobiology Unit, UMR CNRS 8576, University of Lille, 59655 Villeneuve d'Ascq, France. philippe.delannoy@univ-lille1.fr.
Philippe
P
Delannoy
Structural and Functional Glycobiology Unit, UMR CNRS 8576, University of Lille, 59655 Villeneuve d'Ascq, France. sylvain.julien@univ-lille1.fr.|Cell Plasticity and Cancer, INSERM U908, University of Lille, 59655 Villeneuve d'Ascq, France. sylvain.julien@univ-lille1.fr.
Sylvain
S
Julien
D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D005260:Female; D005677:G(M1) Ganglioside / Q000031:analogs & derivatives* / Q000378:metabolism; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D012799:Sialyltransferases / Q000235:genetics* / Q000378:metabolism
C064020:ganglioside GD1alpha; D005677:G(M1) Ganglioside; D012799:Sialyltransferases; C400189:brain-specific GD1alpha synthase, ST6GalNAc V