An R2R3-MYB Transcription Factor Regulates Eugenol Production in Ripe Strawberry Fruit Receptacles.
Eugenol is a volatile phenylpropanoid that contributes to flower and ripe fruit scent. In ripe strawberry (Fragaria × ananassa) fruit receptacles, eugenol is biosynthesized by eugenol synthase (FaEGS2). However, the transcriptional regulation of this process is still unknown. We have identified and functionally characterized an R2R3 MYB transcription factor (emission of benzenoid II [FaEOBII]) that seems to be the orthologous gene of PhEOBII from Petunia hybrida, which contributes to the regulation of eugenol biosynthesis in petals. The expression of FaEOBII was ripening related and fruit receptacle specific, although high expression values were also found in petals. This expression pattern of FaEOBII correlated with eugenol content in both fruit receptacle and petals. The expression of FaEOBII was repressed by auxins and activated by abscisic acid, in parallel to the ripening process. In ripe strawberry receptacles, where the expression of FaEOBII was silenced, the expression of cinnamyl alcohol dehydrogenase1 and FaEGS2, two structural genes involved in eugenol production, was down-regulated. A subsequent decrease in eugenol content in ripe receptacles was also observed, confirming the involvement of FaEOBII in eugenol metabolism. Additionally, the expression of FaEOBII was under the control of FaMYB10, another R2R3 MYB transcription factor that regulates the early and late biosynthetic genes from the flavonoid/phenylpropanoid pathway. In parallel, the amount of eugenol in FaMYB10-silenced receptacles was also diminished. Taken together, these data indicate that FaEOBII plays a regulating role in the volatile phenylpropanoid pathway gene expression that gives rise to eugenol production in ripe strawberry receptacles.
Departamento de Bioquímica y Biología Molecular, Campus Universitario de Rabanales y Campus de Excelencia Internacional Agroalimentario 3, Universidad de Córdoba, 14071 Cordoba, Spain (L.M.-P., F.J.M.-H., J.L.C., R.B.-P., J.M.-B.);Department of Plant Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, 1098 XH Amsterdam, The Netherlands (M.B., R.C.S.); andGenomics Unit (I.L.-V., J.-M.F.-Z.) and Department of Plant Molecular Genetics (R.S.), Centro Nacional de Biotecnología-Consejo Superior de Investigaciones Científicas, 28049 Madrid, Spain.
Laura
L
Medina-Puche
Departamento de Bioquímica y Biología Molecular, Campus Universitario de Rabanales y Campus de Excelencia Internacional Agroalimentario 3, Universidad de Córdoba, 14071 Cordoba, Spain (L.M.-P., F.J.M.-H., J.L.C., R.B.-P., J.M.-B.);Department of Plant Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, 1098 XH Amsterdam, The Netherlands (M.B., R.C.S.); andGenomics Unit (I.L.-V., J.-M.F.-Z.) and Department of Plant Molecular Genetics (R.S.), Centro Nacional de Biotecnología-Consejo Superior de Investigaciones Científicas, 28049 Madrid, Spain.
Francisco Javier
FJ
Molina-Hidalgo
Departamento de Bioquímica y Biología Molecular, Campus Universitario de Rabanales y Campus de Excelencia Internacional Agroalimentario 3, Universidad de Córdoba, 14071 Cordoba, Spain (L.M.-P., F.J.M.-H., J.L.C., R.B.-P., J.M.-B.);Department of Plant Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, 1098 XH Amsterdam, The Netherlands (M.B., R.C.S.); andGenomics Unit (I.L.-V., J.-M.F.-Z.) and Department of Plant Molecular Genetics (R.S.), Centro Nacional de Biotecnología-Consejo Superior de Investigaciones Científicas, 28049 Madrid, Spain.
Maaike
M
Boersma
Departamento de Bioquímica y Biología Molecular, Campus Universitario de Rabanales y Campus de Excelencia Internacional Agroalimentario 3, Universidad de Córdoba, 14071 Cordoba, Spain (L.M.-P., F.J.M.-H., J.L.C., R.B.-P., J.M.-B.);Department of Plant Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, 1098 XH Amsterdam, The Netherlands (M.B., R.C.S.); andGenomics Unit (I.L.-V., J.-M.F.-Z.) and Department of Plant Molecular Genetics (R.S.), Centro Nacional de Biotecnología-Consejo Superior de Investigaciones Científicas, 28049 Madrid, Spain.
Robert C
RC
Schuurink
Departamento de Bioquímica y Biología Molecular, Campus Universitario de Rabanales y Campus de Excelencia Internacional Agroalimentario 3, Universidad de Córdoba, 14071 Cordoba, Spain (L.M.-P., F.J.M.-H., J.L.C., R.B.-P., J.M.-B.);Department of Plant Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, 1098 XH Amsterdam, The Netherlands (M.B., R.C.S.); andGenomics Unit (I.L.-V., J.-M.F.-Z.) and Department of Plant Molecular Genetics (R.S.), Centro Nacional de Biotecnología-Consejo Superior de Investigaciones Científicas, 28049 Madrid, Spain.
Irene
I
López-Vidriero
Departamento de Bioquímica y Biología Molecular, Campus Universitario de Rabanales y Campus de Excelencia Internacional Agroalimentario 3, Universidad de Córdoba, 14071 Cordoba, Spain (L.M.-P., F.J.M.-H., J.L.C., R.B.-P., J.M.-B.);Department of Plant Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, 1098 XH Amsterdam, The Netherlands (M.B., R.C.S.); andGenomics Unit (I.L.-V., J.-M.F.-Z.) and Department of Plant Molecular Genetics (R.S.), Centro Nacional de Biotecnología-Consejo Superior de Investigaciones Científicas, 28049 Madrid, Spain.
Roberto
R
Solano
Departamento de Bioquímica y Biología Molecular, Campus Universitario de Rabanales y Campus de Excelencia Internacional Agroalimentario 3, Universidad de Córdoba, 14071 Cordoba, Spain (L.M.-P., F.J.M.-H., J.L.C., R.B.-P., J.M.-B.);Department of Plant Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, 1098 XH Amsterdam, The Netherlands (M.B., R.C.S.); andGenomics Unit (I.L.-V., J.-M.F.-Z.) and Department of Plant Molecular Genetics (R.S.), Centro Nacional de Biotecnología-Consejo Superior de Investigaciones Científicas, 28049 Madrid, Spain.
José-Manuel
JM
Franco-Zorrilla
Departamento de Bioquímica y Biología Molecular, Campus Universitario de Rabanales y Campus de Excelencia Internacional Agroalimentario 3, Universidad de Córdoba, 14071 Cordoba, Spain (L.M.-P., F.J.M.-H., J.L.C., R.B.-P., J.M.-B.);Department of Plant Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, 1098 XH Amsterdam, The Netherlands (M.B., R.C.S.); andGenomics Unit (I.L.-V., J.-M.F.-Z.) and Department of Plant Molecular Genetics (R.S.), Centro Nacional de Biotecnología-Consejo Superior de Investigaciones Científicas, 28049 Madrid, Spain.
José Luis
JL
Caballero
Departamento de Bioquímica y Biología Molecular, Campus Universitario de Rabanales y Campus de Excelencia Internacional Agroalimentario 3, Universidad de Córdoba, 14071 Cordoba, Spain (L.M.-P., F.J.M.-H., J.L.C., R.B.-P., J.M.-B.);Department of Plant Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, 1098 XH Amsterdam, The Netherlands (M.B., R.C.S.); andGenomics Unit (I.L.-V., J.-M.F.-Z.) and Department of Plant Molecular Genetics (R.S.), Centro Nacional de Biotecnología-Consejo Superior de Investigaciones Científicas, 28049 Madrid, Spain.
Rosario
R
Blanco-Portales
Departamento de Bioquímica y Biología Molecular, Campus Universitario de Rabanales y Campus de Excelencia Internacional Agroalimentario 3, Universidad de Córdoba, 14071 Cordoba, Spain (L.M.-P., F.J.M.-H., J.L.C., R.B.-P., J.M.-B.);Department of Plant Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, 1098 XH Amsterdam, The Netherlands (M.B., R.C.S.); andGenomics Unit (I.L.-V., J.-M.F.-Z.) and Department of Plant Molecular Genetics (R.S.), Centro Nacional de Biotecnología-Consejo Superior de Investigaciones Científicas, 28049 Madrid, Spain bb1mublj@uco.es.
Juan
J
Muñoz-Blanco
D001483:Base Sequence; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D005054:Eugenol / Q000378:metabolism*; D035264:Flowers / Q000235:genetics; D031985:Fragaria / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D005638:Fruit / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D020868:Gene Silencing / Q000187:drug effects; D017343:Genes, Plant; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D059828:Plant Cells / Q000187:drug effects / Q000378:metabolism; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding / Q000187:drug effects; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D013343:Styrenes / Q000378:metabolism; D013997:Time Factors; D014157:Transcription Factors / Q000378:metabolism*; D015533:Transcriptional Activation / Q000187:drug effects / Q000235:genetics
D010937:Plant Growth Regulators; D010940:Plant Proteins; D013343:Styrenes; D014157:Transcription Factors; D005054:Eugenol