Oxidant and antioxidant status in experimental rat testis after testicular torsion/detorsion.
BACKGROUND
The aim of this study was to determine oxidative stress (OS) parameters after testicular torsion/detorsion in adult rats.


METHODS
In this experimental study, male adult Wistar rats were divided into four groups, each consisting of seven animals: group I-one hour right testicular torsion with subsequent orchiectomy, group II-one hour right testicular torsion followed by detorsion, group III-unilateral right-sided orchiectomy without previous torsion and group IV-control. After 30 days, bilateral orchiectomies were performed in rats with both testes and unilateral orchiectomies in rats with single testicles. Parameters of OS were determined in testicular tissue and in plasma.


RESULTS
Plasma concentrations of advanced oxidation protein products (AOPP) and thiobarbituric acid reactive substances (TBARS) were higher (p<0.05 and p<0.01, respectively), whilst the plasma concentration of the total sulfhydryl (T-SH)-groups was lower (p<0.05) in group I compared to the control group. Group II had higher plasma concentrations of AOPP compared to group IV (p<0.05), as well as significantly increased TBARS and decreased T-SH-group levels compared to groups III (p<0.05 and p<0.01, respectively) and IV (p<0.01, for both parameters). There were significant differences in OS markers between the ipsilateral and contralateral testis, as well as significant correlations among levels of both plasma and tissue markers of OS.


CONCLUSIONS
The increase in TBARS levels seen throughout the experimental period indicated that OS development was caused by ischemia/reperfusion in the testicular tissue. The oxidant-antioxidant system of the testicular tissue was altered during torsion as well as detorsion.
Department of Biochemistry, School of Medicine, University of Nis, Nis, Serbia ; Clinic of Nephrology, Clinical Centre Nis, Nis, Serbia.
Tatjana
T
Cvetkovic
Clinic of Urology, Clinical Centre Nis, Nis, Serbia.
Jablan
J
Stankovic
Department of Biology, School of Medicine, University of Nis, Nis, Serbia.
Stevo
S
Najman
Department of Biochemistry, School of Medicine, University of Nis, Nis, Serbia.
Dusica
D
Pavlovic
Department of Pharmacology and Toxicology, School of Medicine, University of Nis, Nis, Serbia.
Dragana
D
Stokanovic
Department of Anatomy, School of Medicine, University of Nis, Nis, Serbia.
Slobodan
S
Vlajkovic
Department of Anatomy, School of Medicine, University of Nis, Nis, Serbia.
Marija
M
Dakovic-Bjelakovic
Department of Anatomy, School of Medicine, University of Nis, Nis, Serbia.
Jovana
J
Cukuranovic
Department of Anatomy, School of Medicine, University of Nis, Nis, Serbia.
Vladimir
V
Zivkovic
School of Medicine, University of Nis, Nis, Serbia.
Vladisav
V
Stefanovic
Advanced Oxidation Protein Products; Oxidative Stress; Reperfusion Injury; Spermatic Cord Torsion; Testis