Increased expression of IL-28RA mRNA in peripheral blood mononuclear cells from patients with systemic lupus erythematosus.
Systemic lupus erythematosus (SLE) is a systemic autoimmune and inflammatory disease with a strong genetic contribution and characterized by kinds of immune reactions. Our previous genome-wide association studies have identified IL-28RA as a susceptibility gene for SLE. In this study, we performed a quantitative reverse transcription polymerase chain reaction (RT-PCR) in 62 patients with SLE and 69 controls to investigate the different expression levels of IL-28RA in peripheral blood mononuclear cells (PBMCs) from SLE patients and healthy controls and the association between IL-28RA expression and systemic lupus erythematosus disease activity index (SLEDAI) or the variant of the single-nucleotide polymorphism (SNP) rs4649203. The expression levels of IL-28RA messenger RNA (mRNA) in SLE patients were significantly increased compared with those of healthy controls. In addition, there were also significant differences in the expression levels of IL-28RA between active (SLEDAI ≥ 6) or inactive (SLEDAI < 6) SLE groups and healthy controls. However, no correlation was observed between IL-28RA mRNA expression level and SLEDAI. There was no association between the variant of the SNP rs4649203 and IL-28RA mRNA expression levels neither. These results indicated that expression of IL-28RA mRNA may be correlated with the pathogenesis of SLE.
Institute of Dermatology and Department of Dermatology, The First Affiliated Hospital, Anhui Medical University, Meishan Road, Hefei, 230032, Anhui, China.
Yu-Yan
YY
Cheng
Institute of Dermatology and Department of Dermatology, The First Affiliated Hospital, Anhui Medical University, Meishan Road, Hefei, 230032, Anhui, China.
Yu-Jun
YJ
Sheng
Institute of Dermatology and Department of Dermatology, The First Affiliated Hospital, Anhui Medical University, Meishan Road, Hefei, 230032, Anhui, China.
Yan
Y
Chang
Institute of Dermatology and Department of Dermatology, The First Affiliated Hospital, Anhui Medical University, Meishan Road, Hefei, 230032, Anhui, China.
Yan
Y
Lin
Institute of Dermatology and Department of Dermatology, The First Affiliated Hospital, Anhui Medical University, Meishan Road, Hefei, 230032, Anhui, China.
Zheng-Wei
ZW
Zhu
Institute of Dermatology and Department of Dermatology, The First Affiliated Hospital, Anhui Medical University, Meishan Road, Hefei, 230032, Anhui, China.
Lei-Lei
LL
Wen
Institute of Dermatology and Department of Dermatology, The First Affiliated Hospital, Anhui Medical University, Meishan Road, Hefei, 230032, Anhui, China.
Chao
C
Yang
Institute of Dermatology and Department of Dermatology, The First Affiliated Hospital, Anhui Medical University, Meishan Road, Hefei, 230032, Anhui, China.
Lu
L
Liu
Institute of Dermatology and Department of Dermatology, The First Affiliated Hospital, Anhui Medical University, Meishan Road, Hefei, 230032, Anhui, China.
Lu-Lu
LL
Yang
Institute of Dermatology and Department of Dermatology, The First Affiliated Hospital, Anhui Medical University, Meishan Road, Hefei, 230032, Anhui, China.
Fu-Sheng
FS
Zhou
Institute of Dermatology and Department of Dermatology, The First Affiliated Hospital, Anhui Medical University, Meishan Road, Hefei, 230032, Anhui, China.
Xiao-Dong
XD
Zheng
Institute of Dermatology and Department of Dermatology, The First Affiliated Hospital, Anhui Medical University, Meishan Road, Hefei, 230032, Anhui, China.
Xian-Yong
XY
Yin
Institute of Dermatology and Department of Dermatology, The First Affiliated Hospital, Anhui Medical University, Meishan Road, Hefei, 230032, Anhui, China.
Sheng-Quan
SQ
Zhang
Department of Dermatology, China-Japan Friendship Hospital, Beijing, 100029, China. wuhucuiyong@vip.163.com.
Yong
Y
Cui
Institute of Dermatology and Department of Dermatology, The First Affiliated Hospital, Anhui Medical University, Meishan Road, Hefei, 230032, Anhui, China. yangsen@medmail.com.cn.
Sen
S
Yang
Institute of Dermatology and Department of Dermatology, The First Affiliated Hospital, Anhui Medical University, Meishan Road, Hefei, 230032, Anhui, China.
Xue-Jun
XJ
Zhang
D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D008180:Lupus Erythematosus, Systemic / Q000097:blood*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012333:RNA, Messenger / Q000378:metabolism; D018121:Receptors, Cytokine / Q000097:blood*; D017471:Receptors, Interferon; D020133:Reverse Transcriptase Polymerase Chain Reaction
C579640:IFNLR1 protein, human; D012333:RNA, Messenger; D018121:Receptors, Cytokine; D017471:Receptors, Interferon
IL-28RA; Quantitative reverse transcription polymerase chain reaction; Single-nucleotide polymorphism; Systemic lupus erythematosus