Improvement of crocin stability by biodegradeble nanoparticles of chitosan-alginate.
This study aimed to improve the stability of crocin, a saffron carotenoid, encapsulating into chitosan (Cs)-sodium alginate (Alg) nanoparticles prepared by a modified ionic gelation method were investigated as a new carrier to improve the stability of crocin. Response surface methodology was used to optimize the important variables, namely the concentrations of Cs and Alg, and pH influencing the particle size, zeta-potential, and encapsulation efficiency to find the optimum formulation for production of crocin nanoparticles (CNPs). Microscopic analysis and dynamic light scattering examination indicated non-smooth and spherical nanoparticles with the size range of 165-230 nm in weight ratio of Cs:Alg (1:1.25) and pH 4.7. Fourier transform-infrared spectroscopy displayed an extensive hydrogen bonding interaction between the crocin and biopolymers. Encapsulation efficiency, loading capacity and yield of CNPs were 38.16, 30.96 and 48.33%, respectively. The zeta-potential of NPs was about -33.52 mV which resulted in the better stability of NPs during manipulation and storage. Stability studies showed that nanoencapsulation provided enhanced crocin stability with biopolymers compared to the standard crocin under unfavorable environmental conditions.
Department of Food Science, Engineering and Technology, Faculty of Agricultural Engineering and Technology, University of Tehran, P.O. Box 4111, Karaj 31587-77871, Iran.
Somayeh
S
Rahaiee
Biotechnology Group, Department of Chemical Engineering, Tarbiat Modares University, Tehran, Iran. Electronic address: shoja_sa@modares.ac.ir.
Seyed Abbas
SA
Shojaosadati
Department of Microbial Biotechnology and Biosafety, Agricultural Biotechnology Research Institute of Iran (ABRII), AREEO, 3135933151 Karaj, Iran. Electronic address: hashemim@abrii.ac.ir.
Maryam
M
Hashemi
Department of Food Science, Engineering and Technology, Faculty of Agricultural Engineering and Technology, University of Tehran, P.O. Box 4111, Karaj 31587-77871, Iran.
Sohrab
S
Moini
Department of Food Science, Engineering and Technology, Faculty of Agricultural Engineering and Technology, University of Tehran, P.O. Box 4111, Karaj 31587-77871, Iran.
Seyed Hadi
SH
Razavi
D000464:Alginates / Q000737:chemistry*; D002338:Carotenoids / Q000737:chemistry* / Q000302:isolation & purification; D048271:Chitosan / Q000737:chemistry*; D027622:Crocus / Q000737:chemistry*; D004337:Drug Carriers; D004339:Drug Compounding; D004355:Drug Stability; D005163:Factor Analysis, Statistical; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000302:isolation & purification; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size
D000464:Alginates; D004337:Drug Carriers; D016166:Free Radical Scavengers; D006603:Hexuronic Acids; D002338:Carotenoids; C029036:crocin; D020723:Glucuronic Acid; D048271:Chitosan
Biopolymers nanoparticles; Crocin; Stability