Characterizing the in vivo role of trehalose in Saccharomyces cerevisiae using the AGT1 transporter.
Trehalose is a highly stable, nonreducing disaccharide of glucose. A large body of research exists implicating trehalose in a variety of cellular phenomena, notably response to stresses of various kinds. However, in very few cases has the role of trehalose been examined directly in vivo. Here, we describe the development and characterization of a system in Saccharomyces cerevisiae that allows us to manipulate intracellular trehalose concentrations independently of the biosynthetic enzymes and independently of any applied stress. We found that many physiological roles heretofore ascribed to intracellular trehalose, including heat resistance, are not due to the presence of trehalose per se. We also found that many of the metabolic and growth defects associated with mutations in the trehalose biosynthesis pathway are not abolished by providing abundant intracellular trehalose. Instead, we made the observation that intracellular accumulation of trehalose or maltose (another disaccharide of glucose) is growth-inhibitory in a carbon source-specific manner. We conclude that the physiological role of the trehalose pathway is fundamentally metabolic: i.e., more complex than simply the consequence of increased concentrations of the sugar and its attendant physical properties (with the exception of the companion paper where Tapia et al. [Tapia H, et al. (2015) Proc Natl Acad Sci USA, 10.1073/pnas.1506415112] demonstrate a direct role for trehalose in protecting cells against desiccation).
Lewis-Sigler Institute for Integrative Genomics and Departments of Molecular Biology and.
Patrick A
PA
Gibney
Lewis-Sigler Institute for Integrative Genomics and.
Ariel
A
Schieler
Lewis-Sigler Institute for Integrative Genomics and Chemistry, Princeton University, Princeton, NJ 08544.
Jonathan C
JC
Chen
Lewis-Sigler Institute for Integrative Genomics and Chemistry, Princeton University, Princeton, NJ 08544.
Joshua D
JD
Rabinowitz
Lewis-Sigler Institute for Integrative Genomics and Departments of Molecular Biology and botstein@princeton.edu.
David
D
Botstein
D001692:Biological Transport; D002244:Carbon / Q000378:metabolism; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D005947:Glucose / Q000378:metabolism; D006358:Hot Temperature; D008320:Maltose / Q000378:metabolism; D055432:Metabolomics; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D027981:Symporters / Q000378:metabolism*; D014199:Trehalose / Q000378:metabolism*
D009004:Monosaccharide Transport Proteins; D029701:Saccharomyces cerevisiae Proteins; D027981:Symporters; C067575:maltose transport system, S cerevisiae; D008320:Maltose; D002244:Carbon; D014199:Trehalose; D005947:Glucose
AGT1; carbon source; heat shock; trehalose; yeast