Microbial communities, extracellular proteomics and polysaccharides: A comparative investigation on biofilm and suspended sludge.
Biofilm and suspended sludge (S-sludge) floc exhibit distinct physicochemical properties and process performances in an integrated fixed-film and suspended growth sequencing batch reactor. However, the mechanisms of governing these differences between the two aggregates were unknown. Current work evaluated the diversity of morphologies, microbial communities, extracellular proteins and polysaccharides between the biofilm and S-sludge. Contrast to biofilm, the denitrification was much more extensive performed in S-sludge. Furthermore, many microbial cells in the biofilm acted as the backbone of aggregates and maintained the structure stability. An extracellular protein observed only in the biofilm can promote the cell adhesion. In contrast, more extracellular proteins related to catalytic activity in the S-sludge could decrease the compactness of floc. In addition, the monosaccharide compositions from the two aggregates were various. These results could elucidate how the diversities of architecture and biochemical process between the two aggregates occurred.
Key Laboratory of the Three Gorges Reservoir Region's Eco-Environments of MOE, Chongqing University, Chongqing 400045, China.
Peng
P
Zhang
Key Laboratory of the Three Gorges Reservoir Region's Eco-Environments of MOE, Chongqing University, Chongqing 400045, China; Key Laboratory of Reservoir Aquatic Environment of CAS, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China.
Jin-Song
JS
Guo
Key Laboratory of Reservoir Aquatic Environment of CAS, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China.
Yu
Y
Shen
Key Laboratory of Reservoir Aquatic Environment of CAS, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China.
Peng
P
Yan
Key Laboratory of the Three Gorges Reservoir Region's Eco-Environments of MOE, Chongqing University, Chongqing 400045, China; Key Laboratory of Reservoir Aquatic Environment of CAS, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China. Electronic address: ypchen@cigit.ac.cn.
You-Peng
YP
Chen
Key Laboratory of the Three Gorges Reservoir Region's Eco-Environments of MOE, Chongqing University, Chongqing 400045, China.
Han
H
Wang
Key Laboratory of Reservoir Aquatic Environment of CAS, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China.
Ji-Xiang
JX
Yang
Key Laboratory of the Three Gorges Reservoir Region's Eco-Environments of MOE, Chongqing University, Chongqing 400045, China.
Fang
F
Fang
Key Laboratory of the Three Gorges Reservoir Region's Eco-Environments of MOE, Chongqing University, Chongqing 400045, China.
Chun
C
Li
D001419:Bacteria / Q000145:classification / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D018441:Biofilms / Q000254:growth & development*; D019149:Bioreactors / Q000382:microbiology; D059013:Microbial Consortia / Q000502:physiology*; D011135:Polysaccharides, Bacterial / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism; D012722:Sewage / Q000382:microbiology*
D001426:Bacterial Proteins; D011135:Polysaccharides, Bacterial; D020543:Proteome; D012722:Sewage
Biofilm; Exopolysaccharide; Extracellular protein; Microbial community; Suspended sludge