title: Proteomics analysis in frozen horse mackerel previously high-pressure processed.
abstract: The effect of high-pressure processing (HPP) (150, 300 and 450 MPa for 0, 2.5 and 5 min) on total sodium dodecyl sulphate (SDS)-soluble and sarcoplasmic proteins in frozen (-10 °C for 3 months) horse mackerel (Trachurus trachurus) was evaluated. Proteomics tools based on image analysis of SDS-PAGE protein gels and protein identification by tandem mass spectrometry (MS/MS) were applied. Although total SDS-soluble fraction indicated no important changes induced by HPP, this processing modified the 1-D SDS-PAGE sarcoplasmic patterns in a direct-dependent manner and exerted a selective effect on particular proteins depending on processing conditions. Thus, application of the highest pressure (450 MPa) provoked a significant degradation of phosphoglycerate mutase 2, glycogen phosphorylase muscle form, pyruvate kinase muscle isozyme, beta-enolase and triosephosphate isomerase and phosphoglucomutase-1. Conversely, protein bands assigned to tropomyosin alpha-1 chain, fast myotomal muscle troponin T and parvalbumin beta 2 increased their intensity after applying a 450-MPa processing.
authors.affiliation: Department of Food Technology, Instituto de Investigaciones Marinas (IIM-CSIC), Calle Eduardo Cabello, 6, 36208 Vigo, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Pazos
authors.affiliation: Department of Food Technology, Instituto de Investigaciones Marinas (IIM-CSIC), Calle Eduardo Cabello, 6, 36208 Vigo, Spain.
authors.forename: Lucía
authors.initials: L
authors.lastname: Méndez
authors.affiliation: Department of Analytical Chemistry, Faculty of Veterinary Science, University of Santiago de Compostela, Avenida Carballo Calero, s/n, 36208 Lugo, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Vázquez
authors.affiliation: Department of Food Technology, Instituto de Investigaciones Marinas (IIM-CSIC), Calle Eduardo Cabello, 6, 36208 Vigo, Spain. Electronic address: saubourg@iim.csic.es.
authors.forename: Santiago P
authors.initials: SP
authors.lastname: Aubourg
mesh_terms: D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005504:Food Analysis; D005511:Food Handling; D061353:Food Storage; D005615:Freezing; D009124:Muscle Proteins / Q000737:chemistry; D010473:Perciformes; D011312:Pressure; D040901:Proteomics; D017747:Seafood / Q000032:analysis*; D053719:Tandem Mass Spectrometry
chemical_list: D009124:Muscle Proteins
keywords: Frozen storage; High pressure; Proteomics; SDS-soluble proteins; Sarcoplasmic proteins; Trachurus trachurus