title: Deoxycholate-Based Glycosides (DCGs) for Membrane Protein Stabilisation.
abstract: Detergents are an absolute requirement for studying the structure of membrane proteins. However, many conventional detergents fail to stabilise denaturation-sensitive membrane proteins, such as eukaryotic proteins and membrane protein complexes. New amphipathic agents with enhanced efficacy in stabilising membrane proteins will be helpful in overcoming the barriers to studying membrane protein structures. We have prepared a number of deoxycholate-based amphiphiles with carbohydrate head groups, designated deoxycholate-based glycosides (DCGs). These DCGs are the hydrophilic variants of previously reported deoxycholate-based N-oxides (DCAOs). Membrane proteins in these agents, particularly the branched diglucoside-bearing amphiphiles DCG-1 and DCG-2, displayed favourable behaviour compared to previously reported parent compounds (DCAOs) and conventional detergents (LDAO and DDM). Given their excellent properties, these agents should have significant potential for membrane protein studies.
authors.affiliation: Department of Bionanotechnology, Hanyang University, Hanyangdaehak-ro, Ansan, 426-791 (Korea).
authors.forename: Hyoung Eun
authors.initials: HE
authors.lastname: Bae
authors.affiliation: Department of Neuroscience and Pharmacology, University of Copenhagen, Nørre Alle 20, 2200 Copenhagen (Denmark).|Present address: Department of Biomedical Sciences, University of Copenhagen, Nørre Alle 20, 2200 Copenhagen (Denmark).
authors.forename: Kamil
authors.initials: K
authors.lastname: Gotfryd
authors.affiliation: Present address: MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Francis Crick Avenue, Cambridge, CB2 OQH (UK).|Department of Life Sciences, Imperial College London, Exhibition Road, London, SW7 2AZ (UK).
authors.forename: Jennifer
authors.initials: J
authors.lastname: Thomas
authors.affiliation: Department of Bionanotechnology, Hanyang University, Hanyangdaehak-ro, Ansan, 426-791 (Korea).
authors.forename: Hazrat
authors.initials: H
authors.lastname: Hussain
authors.affiliation: Department of Bionanotechnology, Hanyang University, Hanyangdaehak-ro, Ansan, 426-791 (Korea).
authors.forename: Muhammad
authors.initials: M
authors.lastname: Ehsan
authors.affiliation: Department of Bionanotechnology, Hanyang University, Hanyangdaehak-ro, Ansan, 426-791 (Korea).
authors.forename: Juyeon
authors.initials: J
authors.lastname: Go
authors.affiliation: Department of Neuroscience and Pharmacology, University of Copenhagen, Nørre Alle 20, 2200 Copenhagen (Denmark).
authors.forename: Claus J
authors.initials: CJ
authors.lastname: Loland
authors.affiliation: Department of Life Sciences, Imperial College London, Exhibition Road, London, SW7 2AZ (UK).
authors.forename: Bernadette
authors.initials: B
authors.lastname: Byrne
authors.affiliation: Department of Bionanotechnology, Hanyang University, Hanyangdaehak-ro, Ansan, 426-791 (Korea). pchae@hanyang.ac.kr.
authors.forename: Pil Seok
authors.initials: PS
authors.lastname: Chae
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D003840:Deoxycholic Acid / Q000737:chemistry*; D003902:Detergents / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D045342:Light-Harvesting Protein Complexes / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry*; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry*; D055550:Protein Stability; D016354:Rhodobacter capsulatus / Q000737:chemistry*; D012995:Solubility
chemical_list: D001426:Bacterial Proteins; D003902:Detergents; D006027:Glycosides; D045342:Light-Harvesting Protein Complexes; D008565:Membrane Proteins; D045322:Photosynthetic Reaction Center Complex Proteins; D003840:Deoxycholic Acid
keywords: amphiphiles; deoxycholate; membrane proteins; molecular design; stabilization