title: Extraction of superoxide dismutase, catalase, and carbonic anhydrase from stroma-free red blood cell hemolysate for the preparation of the nanobiotechnological complex of polyhemoglobin-superoxide dismutase-catalase-carbonic anhydrase.
abstract: We report a novel method to simultaneously extract superoxide dismutase (SOD), catalase (CAT), and carbonic anhydrase (CA) from the same sample of red blood cells (RBCs). This avoids the need to use expensive commercial enzymes, thus enabling a cost-effective process for large-scale production of a nanobiotechnological polyHb-SOD-CAT-CA complex, with enhancement of all three red blood cell functions. An optimal concentration of phosphate buffer for ethanol-chloroform treatment results in good recovery of CAT, SOD, and CA after extraction. Different concentrations of the enzymes can be used to enhance the activity of polyHb-SOD-CAT-CA to 2, 4, or 6 times that of RBC.
authors.affiliation: Artificial Cells and Organs Research Centre, Departments of Physiology, Medicine and Biomedical Engineering, Faculty of Medicine, McGill University , Montreal, Quebec , Canada.
authors.forename: C
authors.initials: C
authors.lastname: Guo
authors.forename: M
authors.initials: M
authors.lastname: Gynn
authors.forename: T M S
authors.initials: TM
authors.lastname: Chang
mesh_terms: D000818:Animals; D001709:Biotechnology; D002256:Carbonic Anhydrases / Q000737:chemistry / Q000302:isolation & purification; D002374:Catalase / Q000737:chemistry / Q000302:isolation & purification; D002417:Cattle; D004912:Erythrocytes / Q000201:enzymology*; D006454:Hemoglobins / Q000138:chemical synthesis / Q000737:chemistry; D006461:Hemolysis; D013482:Superoxide Dismutase / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D006454:Hemoglobins; C037158:polyhemoglobin; D002374:Catalase; D013482:Superoxide Dismutase; D002256:Carbonic Anhydrases
keywords: antioxidant; blood substitutes; carbonic anhydrase; catalase; enzyme extraction; erythrocytes; nanobiotechnology; nanomedicine; oxygen carrier; polyhemoglobin; stroma-free hemolysate; superoxide dismutase