title: Protein trans-splicing as a protein ligation tool to study protein structure and function.
abstract: Protein trans-splicing (PTS) exerted by split inteins is a protein ligation reaction which enables overcoming the barriers of conventional heterologous protein production. We provide an overview of the current state-of-the-art in split intein engineering, as well as the achievements of PTS technology in the realm of protein structure-function analyses, including incorporation of natural and artificial protein modifications, controllable protein reconstitution, segmental isotope labeling and protein cyclization. We further discuss factors crucial for the successful implementation of PTS in these protein engineering approaches, and speculate on necessary future endeavours to make PTS a universally applicable protein ligation tool.
authors.forename: A Sesilja
authors.initials: AS
authors.lastname: Aranko
authors.forename: Gerrit
authors.initials: G
authors.lastname: Volkmann