title: Effects of AFP gene silencing on Survivin mRNA expression inhibition in HepG2 cells.
abstract: We investigated the effects of alpha-fetoprotein (AFP) gene silencing on Survivin expression in HepG2 cells. Small interfering RNA technology was used to downregulate AFP expression in HepG2 cells. An enzyme-linked immunosorbent assay was used to measure AFP concentration in the supernatant before and after transfection. An MTT assay was used to detect cell proliferation activity before and after transfection. We performed flow cytometric analysis to detect the cell apoptosis rate, and reverse transcription-polymerase chain reaction to detect Survivin mRNA levels before and after transfection. Forty-eight hours after transfection, AFP concentration in the supernatant of the experimental group significantly decreased, hepatocellular carcinoma cell growth was inhibited by 43.1%, and the apoptosis rate increased by 24.3%. Survivin mRNA expression was reduced by 78.0% in HepG2 cells. These indicators in the control group and in the blank group did not change significantly. Silencing of AFP expression in HepG2 cells can effectively inhibit the growth of hepatoma cells and promote apoptosis, which may be useful for reducing intracellular Survivin mRNA levels.
authors.affiliation: Department of Medical Oncology, The First Affiliated Hospital of Nanchang University, Nanchang, China.
authors.forename: Z L
authors.initials: ZL
authors.lastname: Fang
authors.affiliation: Gastrointestinal Department of Internal Medicine, The Fourth Affiliated Hospital of Nanchang University, Nanchang, China fangnian_fn@yeah.net.
authors.forename: N
authors.initials: N
authors.lastname: Fang
authors.affiliation: Department of Gynecology and Obstetrics, The Fourth Affiliated Hospital of Nanchang University, Nanchang, China.
authors.forename: X N
authors.initials: XN
authors.lastname: Han
authors.affiliation: Gastrointestinal Department of Internal Medicine, The Fourth Affiliated Hospital of Nanchang University, Nanchang, China.
authors.forename: G
authors.initials: G
authors.lastname: Huang
authors.affiliation: Gastrointestinal Department of Internal Medicine, The Fourth Affiliated Hospital of Nanchang University, Nanchang, China.
authors.forename: X J
authors.initials: XJ
authors.lastname: Fu
authors.affiliation: Gastrointestinal Department of Internal Medicine, The Fourth Affiliated Hospital of Nanchang University, Nanchang, China.
authors.forename: G S
authors.initials: GS
authors.lastname: Xie
authors.affiliation: Gastrointestinal Department of Internal Medicine, The Fourth Affiliated Hospital of Nanchang University, Nanchang, China.
authors.forename: N R
authors.initials: NR
authors.lastname: Wang
authors.affiliation: Department of Medical Oncology, The First Affiliated Hospital of Nanchang University, Nanchang, China.
authors.forename: J P
authors.initials: JP
authors.lastname: Xiong
mesh_terms: D017209:Apoptosis / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D049109:Cell Proliferation / Q000235:genetics; D002470:Cell Survival / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D015972:Gene Expression Regulation, Neoplastic; D056945:Hep G2 Cells; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics* / Q000378:metabolism; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000077022:Survivin; D013997:Time Factors; D000509:alpha-Fetoproteins / Q000235:genetics* / Q000378:metabolism
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D012333:RNA, Messenger; D000077022:Survivin; D000509:alpha-Fetoproteins