title: IL-10 and ARG-1 concentrations in bone marrow and peripheral blood of metastatic neuroblastoma patients do not associate with clinical outcome.
abstract: The expression of the immunosuppressive molecules IL-10 and arginase 1 (ARG-1), and of FOXP3 and CD163, as markers of regulatory T cells (Treg) and macrophages, respectively, was evaluated in bone marrow (BM) and peripheral blood (PB) samples collected at diagnosis from patients with metastatic neuroblastoma (NB). IL-10 and ARG-1 plasma concentrations were measured and the association of each parameter with patients' outcome was tested. The percentages of immunosuppressive Treg and type-1 regulatory (Tr1) cells were also determined. In both BM and PB samples, IL-10 mRNA expression was higher in metastatic NB patients than in controls. IL-10 plasma concentration was higher in patients with NB regardless of stage. Neither IL-10 expression nor IL-10 plasma concentration significantly associated with patient survival. In PB samples from metastatic NB patients, ARG-1 and CD163 expression was higher than in controls but their expression did not associate with survival. Moreover, ARG-1 plasma concentration was lower than in controls, and no association with patient outcome was found. Finally, in metastatic NB patients, the percentage of circulating Treg was higher than in controls, whereas that of Tr1 cells was lower. In conclusion, although IL-10 concentration and Treg percentage were increased, their contribution to the natural history of metastatic NB appears uncertain.
authors.affiliation: Laboratorio di Oncologia IRCCS Istituto Giannina Gaslini, 16148 Genova, Italy.
authors.forename: Fabio
authors.identifier: 0000-0002-2849-7595
authors.initials: F
authors.lastname: Morandi
authors.affiliation: Laboratorio di Bioterapia, IRCCS AOU San Martino-Istituto Nazionale per la Ricerca sul Cancro, 16132 Genova, Italy.
authors.forename: Michela
authors.initials: M
authors.lastname: Croce
authors.affiliation: Laboratorio di Patologica Clinica IRCCS Istituto Giannina Gaslini, 16148 Genova, Italy.
authors.forename: Giuliana
authors.initials: G
authors.lastname: Cangemi
authors.affiliation: Laboratorio di Patologica Clinica IRCCS Istituto Giannina Gaslini, 16148 Genova, Italy.
authors.forename: Sebastiano
authors.identifier: 0000-0002-1148-427X
authors.initials: S
authors.lastname: Barco
authors.affiliation: Laboratorio di Bioterapia, IRCCS AOU San Martino-Istituto Nazionale per la Ricerca sul Cancro, 16132 Genova, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Rigo
authors.affiliation: Laboratorio di Oncologia IRCCS Istituto Giannina Gaslini, 16148 Genova, Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Carlini
authors.affiliation: U.O. Oncologia IRCCS Istituto Giannina Gaslini, 16148 Genova, Italy.
authors.forename: Loredana
authors.initials: L
authors.lastname: Amoroso
authors.affiliation: Laboratorio di Oncologia IRCCS Istituto Giannina Gaslini, 16148 Genova, Italy.
authors.forename: Vito
authors.identifier: 0000-0003-0528-9563
authors.initials: V
authors.lastname: Pistoia
authors.affiliation: Laboratorio di Bioterapia, IRCCS AOU San Martino-Istituto Nazionale per la Ricerca sul Cancro, 16132 Genova, Italy.
authors.forename: Silvano
authors.initials: S
authors.lastname: Ferrini
authors.affiliation: Laboratorio di Oncologia IRCCS Istituto Giannina Gaslini, 16148 Genova, Italy.
authors.forename: Maria Valeria
authors.identifier: 0000-0002-7316-0772
authors.initials: MV
authors.lastname: Corrias
mesh_terms: D015703:Antigens, CD / Q000276:immunology / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000276:immunology / Q000378:metabolism; D001119:Arginase / Q000097:blood* / Q000276:immunology / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000097:blood; D001853:Bone Marrow / Q000378:metabolism / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D051858:Forkhead Transcription Factors / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007223:Infant; D016753:Interleukin-10 / Q000097:blood* / Q000235:genetics / Q000378:metabolism; D018655:Lymphocyte Count; D008264:Macrophages / Q000276:immunology*; D009360:Neoplastic Cells, Circulating; D009447:Neuroblastoma / Q000097:blood* / Q000401:mortality / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis; D011956:Receptors, Cell Surface / Q000276:immunology / Q000378:metabolism; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D016896:Treatment Outcome; D000098902:CD163 Antigen
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D014408:Biomarkers, Tumor; D000098902:CD163 Antigen; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; C508609:IL10 protein, human; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D016753:Interleukin-10; C574619:ARG1 protein, human; D001119:Arginase