title: Combination of Loop-Mediated Isothermal Ampliﬁcation Assay and Nested PCR for Detection of Borrelia burgdorferi sensu lato in Human Serum Samples.
abstract: A set of universal loop-mediated isothermal ampliﬁcation (LAMP) primers targeting the fla gene was designed to detect Borrelia burgdorferi sensu lato (B. burgdorferi s.l.) in human samples. The sensitivity of LAMP was 20 copies/reaction, and the assay did not detect false positives among 11 other related bacteria. A positive LAMP result was obtained for 9 of the 24 confirmed cases and for 12 of 94 suspected cases. The positive rate of LAMP was the same as that of nested PCR. The LAMP is a useful diagnostic method that can be developed for rapid detection of B. burgdorferi s.l. in human sera. Combination of the LAMP and nested PCR was more sensitive for detecting B. burgdorferi s.l. in human serum samples.
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Department of Laboratory Medicine, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan, China; School of Laboratory Medicine, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China.
authors.forename: Liu Li
authors.initials: LL
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, Zhejiang, China.
authors.forename: Xue Xia
authors.initials: XX
authors.lastname: Hou
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, Zhejiang, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Geng
authors.affiliation: .
authors.forename: Yong Liang
authors.initials: YL
authors.lastname: Lou
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, Zhejiang, China.
authors.forename: Kang Lin
authors.initials: KL
authors.lastname: Wan
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, Zhejiang, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Hao
mesh_terms: D015748:Borrelia burgdorferi Group / Q000235:genetics / Q000302:isolation & purification*; D002681:China; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008193:Lyme Disease / Q000175:diagnosis; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D004269:DNA, Bacterial