title: Determination of kynurenic acid in rat cerebrospinal fluid by HPLC with fluorescence detection.
abstract: A sensitive HPLC method using fluorescence detection was developed to determine kynurenic acid (KYNA) level in rat cerebrospinal fluid (CSF). The method development was accomplished by screening different columns, optimizing zinc acetate concentration and determining the optimal HPLC flow rate. This method allowed direct injection of the CSF samples onto an Xselect C18 column and KYNA levels were measured fluorometrically by forming a fluorescent complex with zinc acetate that was delivered post-column. The limit of quantitation was 0.2 n m with 30 μL injection, corresponding to 6 fmol (signal-to-noise ratio = 10). The improved sensitivity enabled the measurement of KYNA in naive and drug-treated rat CSF.
authors.affiliation: Bioanalytical and Discovery Analytical Science, Bristol-Myers Squibb, 5 Research Parkway, Wallingford, CT, 06492, USA.
authors.forename: Ye
authors.initials: Y
authors.lastname: Bao
authors.affiliation: Bioanalytical and Discovery Analytical Science, Bristol-Myers Squibb, 5 Research Parkway, Wallingford, CT, 06492, USA.
authors.forename: David
authors.initials: D
authors.lastname: Luchetti
authors.affiliation: Bioanalytical and Discovery Analytical Science, Bristol-Myers Squibb, 5 Research Parkway, Wallingford, CT, 06492, USA.
authors.forename: Eric
authors.initials: E
authors.lastname: Schaeffer
authors.affiliation: Bioanalytical and Discovery Analytical Science, Bristol-Myers Squibb, 5 Research Parkway, Wallingford, CT, 06492, USA.
authors.forename: Jingfang
authors.initials: J
authors.lastname: Cutrone
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005470:Fluorometry / Q000379:methods*; D007736:Kynurenic Acid / Q000134:cerebrospinal fluid*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D007736:Kynurenic Acid
keywords: HPLC, rat; cerebrospinal fluid; fluorescence; kynurenic acid