title: Next-generation sequencing identification and characterization of microsatellite markers in Aconitum austrokoreense Koidz., an endemic and endangered medicinal plant of Korea.
abstract: We used next-generation sequencing to develop 9 novel microsatellite markers in Aconitum austrokoreense, an endemic and endangered medicinal plant in Korea. Owing to its very limited distribution, over-harvesting for traditional medicinal purposes, and habitat loss, the natural populations are dramatically declining in Korea. All novel microsatellite markers were successfully genotyped using 64 samples from two populations (Mt. Choejeong, Gyeongsangbuk-do and Ungseokbong, Gyeongsangnam-do) of Gyeongsang Province. The number of alleles ranged from 2 to 7 per locus in each population. Observed and expected heterozygosities ranged from 0.031 to 0.938 and from 0.031 to 0.697, respectively. The novel markers will be valuable tools for assessing the genetic diversity of A. austrokoreense and for germplasm conservation of this endangered species.
authors.affiliation: National Institute of Biological Resources, Environmental Research Complex, Incheon, Korea.
authors.forename: Y-E
authors.initials: YE
authors.lastname: Yun
authors.affiliation: National Institute of Biological Resources, Environmental Research Complex, Incheon, Korea.
authors.forename: J-N
authors.initials: JN
authors.lastname: Yu
authors.affiliation: National Institute of Biological Resources, Environmental Research Complex, Incheon, Korea.
authors.forename: G H
authors.initials: GH
authors.lastname: Nam
authors.affiliation: National Institute of Biological Resources, Environmental Research Complex, Incheon, Korea.
authors.forename: S-A
authors.initials: SA
authors.lastname: Ryu
authors.affiliation: National Institute of Biological Resources, Environmental Research Complex, Incheon, Korea.
authors.forename: S
authors.initials: S
authors.lastname: Kim
authors.affiliation: National Institute of Biological Resources, Environmental Research Complex, Incheon, Korea.
authors.forename: K
authors.initials: K
authors.lastname: Oh
authors.affiliation: National Institute of Biological Resources, Environmental Research Complex, Incheon, Korea Chaelim@korea.kr.
authors.forename: C E
authors.initials: CE
authors.lastname: Lim
mesh_terms: D031841:Aconitum / Q000235:genetics*; D000483:Alleles; D018744:DNA, Plant / Q000235:genetics; D056727:Endangered Species; D005838:Genotype; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D010946:Plants, Medicinal / Q000235:genetics*; D011110:Polymorphism, Genetic; D056910:Republic of Korea
chemical_list: D018744:DNA, Plant