title: Cationic nanoemulsions as potential carriers for intracellular delivery.
abstract: Successful cytosolic delivery enables opportunities for improved treatment of various genetic disorders, infectious diseases and cancer. Cationic nanoemulsions were designed using alternative excipients and evaluated for particle size, charge, effect of sterilization on its stability, DNA condensation potential and cellular uptake efficiency. Various concentrations of non-ionic and ionic stabilizers were evaluated to design formula for colloidally stable cationic nanoemulsion. The nanoemulsion comprised of 5% Capmul MCM, 0.5% didodecyldimethylammonium bromide (DDAB), 1% phospholipid, 1% Poloxamer 188 and 2.25% glycerol and possessed particle size of 81.6 ± 3.56 nm and 137.1 ± 1.57 nm before and after steam sterilization, respectively. DNA condensation studies were carried out at various nanoemulsion: DNA ratios ranging from 1:1 to 10:1. Cell uptake studies were conducted on human embryonic kidney (HEK) cell lines which are widely reported for transfection studies. The nanoemulsions showed excellent cellular uptake as evaluated by fluorescence microscopy and flow cytometry. Overall, a colloidally stable cationic nanoemulsion with good DNA condensation ability was successfully fabricated for efficient cytosolic delivery and potential for in vivo effectiveness.
authors.affiliation: Department of Pharmaceutics, Bombay College of Pharmacy, Kalina, Santacruz (East), Mumbai 400098, India.
authors.forename: P V
authors.initials: PV
authors.lastname: Khachane
authors.affiliation: Department of Pharmaceutics, Bombay College of Pharmacy, Kalina, Santacruz (East), Mumbai 400098, India.
authors.forename: A S
authors.initials: AS
authors.lastname: Jain
authors.affiliation: Department of Pharmaceutics, Bombay College of Pharmacy, Kalina, Santacruz (East), Mumbai 400098, India.
authors.forename: V V
authors.initials: VV
authors.lastname: Dhawan
authors.affiliation: Tata Memorial Center, Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Kharghar, Navi Mumbai 410210, Maharashtra, India.
authors.forename: G V
authors.initials: GV
authors.lastname: Joshi
authors.affiliation: Department of Pharmaceutics, Bombay College of Pharmacy, Kalina, Santacruz (East), Mumbai 400098, India ; School of Pharmacy and Health Professions, Creighton University, Omaha, NE 68178, USA.
authors.forename: A A
authors.initials: AA
authors.lastname: Date
authors.affiliation: Tata Memorial Center, Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Kharghar, Navi Mumbai 410210, Maharashtra, India.
authors.forename: R
authors.initials: R
authors.lastname: Mulherkar
authors.affiliation: Department of Pharmaceutics, Bombay College of Pharmacy, Kalina, Santacruz (East), Mumbai 400098, India.
authors.forename: M S
authors.initials: MS
authors.lastname: Nagarsenker
keywords: Cationic; Cytosolic delivery; Didodecyldimethylammonium bromide; Intracellular delivery; Nanoemulsion