title: [Effects of Down-regulating PPP2R5C Expression on Expression Profile of TAL1-related Regulating Genes in Jurkat Cells].
abstract: OBJECTIVE
  Based on our previous study showing the inhibition of lenkemia T cell proliferation by down-regulating PPP2R5C expression, this study was aimed to analyze the influence of down-regulating PPP2R5 expression via RNA interference on genes relatied with TAL1 signaling pathway by using gene chip technique.
  
  
  METHODS
  The PPP2R5C-siRNA799 was transduced into Jurkat cells by nucleofection, the total RNA was isolated from treated Jurkat cells after culture for 48 hours; the target sequences were prepared by revevse transcription after mRNA purification, and were hybridized with affymetrix gene expression profile chip 3' IVT. The original image data were collected using affymetrix gene chip scanner 3 000, and the gene expression profile was analyzed using gene spring GX 11.0 soflware.
  
  
  RESULTS
  The expression of all 26 genes related with TAL1 signaling pathway was changed, out of which the expression of 15 genes were up-regulated and the expression of 11 genes was down-regulated in PPP2R5C-siRNA 799-transfected Jurkat cells. The genes with significantly up-regulated expression were GATA1, TCF4, XRCC6 and TCF3, while the genes with significantly down-regulated expression were SIN3A and RUNX1.
  
  
  CONCLUSIONS
  The down-regulation of PPP2R5C gene expression in Jurkat cells via RNA interference to a certain degree can inhibit TAL1 signaling pathway genes, thereby suppresses the proliferation of Jurkat cells.
authors.affiliation: Medical Research Center, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, Guangdong Province, China.|Institute of Hematology, Medical collage, Jinan University, Guangzhou 510632, Guangdong Province, China.|Key Laboratory for Regenerative Medicine, Department of Education, Jinan University, Guangzhou 510632, Guangdong Province, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Institute of Hematology, Medical collage, Jinan University, Guangzhou 510632, Guangdong Province, China.
authors.forename: Si-Chu
authors.initials: SC
authors.lastname: Liu
authors.affiliation: Institute of Hematology, Medical collage, Jinan University, Guangzhou 510632, Guangdong Province, China.
authors.forename: Li-Jian
authors.initials: LJ
authors.lastname: Yang
authors.affiliation: Institute of Hematology, Medical collage, Jinan University, Guangzhou 510632, Guangdong Province, China.
authors.forename: Shao-Hua
authors.initials: SH
authors.lastname: Chen
authors.affiliation: Institute of Hematology, Medical collage, Jinan University, Guangzhou 510632, Guangdong Province, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Institute of Hematology, Medical collage, Jinan University, Guangzhou 510632, Guangdong Province, China.
authors.forename: Geng-Xin
authors.initials: GX
authors.lastname: Luo
authors.affiliation: Institute of Hematology, Medical collage, Jinan University, Guangzhou 510632, Guangdong Province, China. E-mail: jnyangqiuli@163.com.|Key Laboratory for Regenerative Medicine, Department of Education, Jinan University, Guangzhou 510632, Guangdong Province, China. E-mail: jnyangqiuli@163.com.
authors.forename: Yang-Qiu
authors.initials: YQ
authors.lastname: Li
mesh_terms: D049109:Cell Proliferation; D015536:Down-Regulation; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D019169:Jurkat Cells; D020411:Oligonucleotide Array Sequence Analysis; D054648:Protein Phosphatase 2; D034622:RNA Interference; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D015398:Signal Transduction; D059467:Transcriptome; D014162:Transfection
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering; C503981:PPP2R5C protein, human; D054648:Protein Phosphatase 2