title: Quantitative Analysis of Segmented Fluorescein Angiography Images for the Follow-up of Choroidal Neovascular Membrane.
abstract: OBJECTIVE
  The aim of this study was to evaluate choroidal neovascular (CNV) lesions with fluorescein angiography (FA) and to identify quantitative parameters and correlate these parameters to treatment outcomes.
  
  
  METHODS
  This institution based cross-sectional study evaluated 30 eyes with active sub-foveal predominantly classic CNV treated with bevacizumab. Pre- and post-injection segmented FA images were analyzed. Lesion area and CNV lesion were manually delineated. Outcome measure was the change 1-month after each injection in different intensity values (0-255 divided in eight regions A [lowest intensity] to H [highest intensity] on a linear scale) in lesion area, perimeter, greatest linear dimension (GLD), area, visual acuity (VA) and central macular thickness (CMT).
  
  
  RESULTS
  At month 3, statistically significant changes from baseline occurred in VA, CMT, lesion area, GLD and perimeter (P < 0.05 all comparisons). Change in CMT from baseline to 3 months postinjection was correlated with change in VA (P = 0.009, r = 0.469) and intensity regions B (P = 0.001, r = -0.565), D (P = 0.001, r = 0.560), E (P = 0.035, r = 0.386). At month 3, change in intensity values 0-63 (A + B) was negatively correlated with CMT (P = 0.001, r = -0.575) and lesion area (P = 0.019, r = -0.427); change in intensity values 64-223 (C-G) was positively correlated with CMT (P = 0.000, r = 0.636) and lesion area (P = 0.002, r = 0.551).
  
  
  CONCLUSIONS
  Decrease in area, GLD, perimeter and area with intensity ≥ 64 on segmented FA were associated with a favorable outcome of treatment. These parameters may be useful adjuncts to existing evaluation techniques during follow-up of CNV.
authors.affiliation: Department of Ophthalmology, North Bengal Medical College, West Bengal, India.
authors.forename: Sambuddha
authors.initials: S
authors.lastname: Ghosh
authors.affiliation: Department of Ophthalmology, RG Kar Medical College, Kolkata, West Bengal, India.
authors.forename: Pampa
authors.initials: P
authors.lastname: Haldar
authors.affiliation: Department of Electrical Engineering, IIT, New Delhi, India.
authors.forename: Prashanth
authors.initials: P
authors.lastname: Ravindran
authors.affiliation: Department of School of Medical Science and Technology, IIT, Kharagpore, West Bengal, India.
authors.forename: Jyotirmoy
authors.initials: J
authors.lastname: Chatterjee
authors.affiliation: Department of School of Medical Science and Technology, IIT, Kharagpore, West Bengal, India.
authors.forename: Sandeep V
authors.initials: SV
authors.lastname: Paranjape
authors.affiliation: Department of Ophthalmology, RIO, Kolkata, West Bengal, India.
authors.forename: Gautam
authors.initials: G
authors.lastname: Bhaduri
mesh_terms: D000368:Aged; D020533:Angiogenesis Inhibitors / Q000627:therapeutic use; D061067:Antibodies, Monoclonal, Humanized / Q000627:therapeutic use; D000068258:Bevacizumab; D020256:Choroidal Neovascularization / Q000175:diagnosis* / Q000188:drug therapy / Q000503:physiopathology; D003430:Cross-Sectional Studies; D005260:Female; D005451:Fluorescein Angiography; D005500:Follow-Up Studies; D006801:Humans; D058449:Intravitreal Injections; D008297:Male; D012189:Retrospective Studies; D016896:Treatment Outcome; D042461:Vascular Endothelial Growth Factor A / Q000037:antagonists & inhibitors; D014792:Visual Acuity / Q000502:physiology
chemical_list: D020533:Angiogenesis Inhibitors; D061067:Antibodies, Monoclonal, Humanized; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D000068258:Bevacizumab
keywords: Age-related Macular Degeneration; Bevacizumab; Choroidal Neovascular Membrane; Fundus Fluorescein Angiography