title: Changes occurring in compositions and antioxidant properties of healthy soybean seeds [Glycine max (L.) Merr.] and soybean seeds diseased by Phomopsis longicolla and Cercospora kikuchii fungal pathogens.
abstract: Changes in the compositions (isoflavone, protein, oil, and fatty acid) and antioxidant properties were evaluated in healthy soybeans and soybeans diseased by Phomopsis longicolla and Cercospora kikuchii. The total isoflavone content (1491.3 μg/g) of healthy seeds was observed to be considerably different than that of diseased seeds (P. longicolla: 292.6, C. kikuchii: 727.2 μg/g), with malonlygenistin exhibiting the greatest decrease (726.1 → 57.1, 351.9 μg/g). Significantly, three isoflavones exhibited a slight increase, and their structures were confirmed as daidzein, glycitein, and genistein, based on their molecular ions at m/z 253.1, 283.0, and 269.1 using the negative mode of HPLC-DAD-ESI/MS. The remaining compositions showed slight variations. The effects against 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) radicals in healthy seeds were stronger than the diseased soybeans, depending upon the isoflavone level. Our results may be useful in evaluating the relationship between composition and antioxidant activity as a result of changes caused by soybean fungal pathogens.
authors.affiliation: Division of Research Development and Education, National Institute of Chemical Safety (NICS), Ministry of Environment, Daejeon 305-343, Republic of Korea.
authors.forename: Jin Hwan
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Division of Research Development and Education, National Institute of Chemical Safety (NICS), Ministry of Environment, Daejeon 305-343, Republic of Korea.
authors.forename: Seung-Ryul
authors.initials: SR
authors.lastname: Hwang
authors.affiliation: Division of Research Development and Education, National Institute of Chemical Safety (NICS), Ministry of Environment, Daejeon 305-343, Republic of Korea.
authors.forename: Yeon-Hee
authors.initials: YH
authors.lastname: Lee
authors.affiliation: Division of Research Development and Education, National Institute of Chemical Safety (NICS), Ministry of Environment, Daejeon 305-343, Republic of Korea.
authors.forename: Kyun
authors.initials: K
authors.lastname: Kim
authors.affiliation: Department of Food Science, Gyeongnam National University of Science and Technology, Jinju 660-758, Republic of Korea. Electronic address: kmcho@gntech.ac.kr.
authors.forename: Kye Man
authors.initials: KM
authors.lastname: Cho
authors.affiliation: Institute of Agriculture & Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea. Electronic address: yblee@gnu.ac.kr.
authors.forename: Yong Bok
authors.initials: YB
authors.lastname: Lee
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D001203:Ascomycota / Q000302:isolation & purification*; D007529:Isoflavones / Q000032:analysis; D010935:Plant Diseases; D010936:Plant Extracts / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry*; D013025:Glycine max / Q000737:chemistry* / Q000382:microbiology
chemical_list: D000975:Antioxidants; D007529:Isoflavones; D010936:Plant Extracts
keywords: Acetyldaidzin (PubChem CID: 156155); Acetylgenistin (PubChem CID: 22288010); Acetylglycitin (PubChem CID: 53398650); Antioxidant activity; Cercospora kikuchii; Composition; Daidzein (PubChem CID: 5281708); Daidzin (PubChem CID: 107971); Genistein (PubChem CID: 5280961); Genistin (PubChem CID: 5281377); Glycitein (PubChem CID: 5317750); Glycitin (PubChem CID: 187808); Isoflavone; Malonyldaidzin (PubChem CID: 9913968); Malonylgenistin (PubChem CID: 90658001); Malonylglycitin (PubChem CID: 23724657); Phomopsis longicolla; Soybean seed