title: Bioabatement with hemicellulase supplementation to reduce enzymatic hydrolysis inhibitors.
abstract: A stepwise removal of inhibitory compounds by bioabatement combined with hemicellulase supplementation was conducted to enhance cellulose hydrolysis of liquid hot water-pretreated corn stover. Results showed that the fungus Coniochaeta ligniaria NRRL30616 eliminated most of the enzyme and fermentation inhibitors from liquid hot water-pretreated corn stover hydrolysates. Moreover, addition of hemicellulases after bioabatement and before enzymatic hydrolysis of cellulose achieved 20% higher glucose yields compared to non-treated samples. This work presents the mechanisms by which supplementation of the fungus with hemicellulase enzymes enables maximal conversion, and confirms the inhibitory effect of xylo-oligosaccharides in corn stover hydrolysates once the dominant inhibitory effect of phenolic compounds is removed.
authors.affiliation: Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, IN 47907-2032, United States; School of Life Science and Technology, Harbin Institute of Technology, Harbin 150001, China; State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150001, China.
authors.forename: Guangli
authors.initials: G
authors.lastname: Cao
authors.affiliation: Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, IN 47907-2032, United States.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Ximenes
authors.affiliation: Bioenergy Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, USDA, 1815 N. University Street, Peoria, IL 61604, United States.
authors.forename: Nancy N
authors.initials: NN
authors.lastname: Nichols
authors.affiliation: Bioenergy Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, USDA, 1815 N. University Street, Peoria, IL 61604, United States.
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Frazer
authors.affiliation: Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, IN 47907-2032, United States.
authors.forename: Daehwan
authors.initials: D
authors.lastname: Kim
authors.affiliation: Bioenergy Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, USDA, 1815 N. University Street, Peoria, IL 61604, United States.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Cotta
authors.affiliation: Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, IN 47907-2032, United States. Electronic address: ladisch@purdue.edu.
authors.forename: Michael
authors.initials: M
authors.lastname: Ladisch
mesh_terms: D001203:Ascomycota / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D006026:Glycoside Hydrolases / Q000737:chemistry*; D006868:Hydrolysis; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry* / Q000378:metabolism*; D003313:Zea mays / Q000382:microbiology*
chemical_list: D004791:Enzyme Inhibitors; D009844:Oligosaccharides; D010936:Plant Extracts; D006026:Glycoside Hydrolases; C023305:hemicellulase
keywords: Bioabatement; Enzymatic hydrolysis; Hemicellulase supplementation; Inhibitors; Liquid hot water biomass pretreatment