title: CRKL knockdown promotes in vitro proliferation, migration and invasion, in vivo tumor malignancy and lymph node metastasis of murine hepatocarcinoma Hca-P cells.
abstract: Our previous study (Biomed Pharmacother 2015;69:11) demonstrated that the over-expression of CRKL, a chicken tumor virus number 10 regulator of kinase-like protein, suppresses in vitro proliferation, invasion and migration of murine hepatocarcinoma Hca-P cell, a murine HCC cell with lymph node metastatic (LNM) rate of ∼25%. In current work, we investigated the effects of CRKL knockdown on the in vitro cell proliferation, migration and invasion, and on the in vivo tumor malignancy and LNM rate and level for Hca-P cells. Western blotting assay indicated that CRKL was down-regulated by ∼90% in a monoclonal CrkL-shRNA-transfected Hca-P cells. Compared with Hca-P and unrelated-shRNA-transfected Hca-P cell, the in vitro proliferation, migration and invasion potentials were significantly enhanced following CRKL stable deregulation. CRKL knock-down significantly promoted the tumorigenicity malignancy, LNM rates and level of Hca-P-transplanted mice. Consistent with our previous work, it can be concluded CRKL plays an important role in hepatocarcinoma cell proliferation, invasion and migration as well hepatocarcinoma malignancy and metastasis. It functions as a potential tumor suppressor in hepatocarcinoma.
authors.affiliation: Department of Biochemistry, Dalian Medical University, Dalian 116044, China; Department of Pathology, The First Affiliated Hospital of China Medical University, Anshan Hospital, Liaoning 114012, China.
authors.forename: Ji
authors.initials: J
authors.lastname: Shi
authors.affiliation: Department of Biochemistry, Dalian Medical University, Dalian 116044, China.
authors.forename: Longlong
authors.initials: L
authors.lastname: Meng
authors.affiliation: Department of Biotechnology, Dalian Medical University, Dalian 116044, China. Electronic address: mingzhongsun@dlmedu.edu.cn.
authors.forename: Ming-Zhong
authors.initials: MZ
authors.lastname: Sun
authors.affiliation: Department of Biotechnology, Dalian Medical University, Dalian 116044, China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Guo
authors.affiliation: Department of Biochemistry, Dalian Medical University, Dalian 116044, China; Department of Biotechnology, Dalian Medical University, Dalian 116044, China.
authors.forename: Xujuan
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Biochemistry, Dalian Medical University, Dalian 116044, China.
authors.forename: Qiuyue
authors.initials: Q
authors.lastname: Lin
authors.affiliation: Department of Biochemistry, Dalian Medical University, Dalian 116044, China; Provincial Key Laboratory of Cell and Molecular Biology, Dalian Medical University, Dalian 116044, China. Electronic address: lsqsmz@163.com.
authors.forename: Shuqing
authors.initials: S
authors.lastname: Liu
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000818:Animals; D063646:Carcinogenesis / Q000473:pathology; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D055785:Gene Knockdown Techniques; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology*; D008207:Lymphatic Metastasis / Q000473:pathology*; D051379:Mice; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D009687:Nuclear Proteins / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000378:metabolism; D014162:Transfection
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C082869:CRKL protein; D009687:Nuclear Proteins; D034741:RNA, Small Interfering
keywords: CRKL; Hepatocarcinoma; Invasion; Metastasis; Migration; Proliferation