title: Incorporation of an acyclic alkynyl nucleoside analog into siRNA improves silencing activity and nuclease resistance.
abstract: In order to improve the silencing activity and nuclease resistance of small interfering RNA (siRNA), we designed and synthesized an acyclic thymidine analog containing 4-pentyne-1,2-diol instead of d-ribofuranose. The incorporation of this analog into siRNAs at specific positions in the strands was found to enhance the silencing activity of siRNAs and to increase the resistance of the siRNA to hydrolytic degradation by a 3' exonuclease.
authors.affiliation: United Graduate School of Agricultural Science, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors.forename: Aya
authors.initials: A
authors.lastname: Ogata
authors.affiliation: United Graduate School of Agricultural Science, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan; Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan. Electronic address: uenoy@gifu-u.ac.jp.
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Ueno
mesh_terms: D001483:Base Sequence; D004720:Endonucleases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D006868:Hydrolysis; D009705:Nucleosides / Q000737:chemistry*; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D034741:RNA, Small Interfering / Q000737:chemistry* / Q000378:metabolism
chemical_list: D009705:Nucleosides; D009841:Oligonucleotides; D034741:RNA, Small Interfering; D004720:Endonucleases
keywords: Acyclic nucleoside; Alkyne; Nuclease resistance; RNAi; siRNA