title: Expression of Perforin Gene for Early Development of Nephrons in Olive Flounder (Paralichthys olivaceus).
abstract: The innate immune system is the only defense weapon that invertebrates have, and it is the fundamental defense mechanism for fish. The innate immune response is important in newly hatched flounders because it is closely involved in the initial feeding phase, which is why it is essential for survival during the juvenile period. The expression analysis of genes involved in the innate immune response in the olive flounder (Paralichthys olivaceus) in the days after hatching is incomplete. Therefore, we have begun to examine the expression patterns of genes specifically induced during the development of the innate immune system in newly hatched flounders. Microscopic observation showed that pronephron formation corresponded with the expression of perforin-encoding gene. These results suggest that perforin plays a vital role in the innate immunity of the kidney during developmental stages. Perforin expression was strong at the start of the development of the innate immune response, and continued throughout all the development stages. Our findings have important implications with respect to perforin's biological role and the evolution of the first defense mechanisms in olive flounder. Further studies are required to elucidate the perforin-mediated innate immunity response and to decipher the functional role of perforin in developmental stages.
authors.affiliation: Genetics and Breeding Research Center, NFRDI, Geoje 656-842, Republic of Korea.
authors.forename: Hyun
authors.initials: H
authors.lastname: Yang
authors.affiliation: Genetics and Breeding Research Center, NFRDI, Geoje 656-842, Republic of Korea.
authors.forename: Young Mee
authors.initials: YM
authors.lastname: Lee
authors.affiliation: Genetics and Breeding Research Center, NFRDI, Geoje 656-842, Republic of Korea.
authors.forename: Jeong-Ho
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Genetics and Breeding Research Center, NFRDI, Geoje 656-842, Republic of Korea.
authors.forename: Jae Koo
authors.initials: JK
authors.lastname: Noh
authors.affiliation: Genetics and Breeding Research Center, NFRDI, Geoje 656-842, Republic of Korea.
authors.forename: Hyun Chul
authors.initials: HC
authors.lastname: Kim
authors.affiliation: Genetics and Breeding Research Center, NFRDI, Geoje 656-842, Republic of Korea.
authors.forename: Choul-Ji
authors.initials: CJ
authors.lastname: Park
authors.affiliation: Genetics and Breeding Research Center, NFRDI, Geoje 656-842, Republic of Korea.
authors.forename: Jong-Won
authors.initials: JW
authors.lastname: Park
authors.affiliation: Genetics and Breeding Research Center, NFRDI, Geoje 656-842, Republic of Korea.
authors.forename: In Joon
authors.initials: IJ
authors.lastname: Hwang
authors.affiliation: Genetics and Breeding Research Center, NFRDI, Geoje 656-842, Republic of Korea.
authors.forename: Sung Yeon
authors.initials: SY
authors.lastname: Kim
keywords: Development; Gene expression; Innate immunity; Olive flounder.; Perforin; Pronephron