title: DNA topology influences molecular machine lifetime in human serum.
abstract: DNA nanotechnology holds the potential for enabling new tools for biomedical engineering, including diagnosis, prognosis, and therapeutics. However, applications for DNA devices are thought to be limited by rapid enzymatic degradation in serum and blood. Here, we demonstrate that a key aspect of DNA nanotechnology-programmable molecular shape-plays a substantial role in device lifetimes. These results establish the ability to operate synthetic DNA devices in the presence of endogenous enzymes and challenge the textbook view of near instantaneous degradation.
authors.affiliation: Department of Materials Science & Engineering, Boise State University, Boise, Idaho 83725, USA. EltonGraugnard@BoiseState.edu.
authors.forename: Sara
authors.initials: S
authors.lastname: Goltry
authors.forename: Natalya
authors.initials: N
authors.lastname: Hallstrom
authors.forename: Tyler
authors.initials: T
authors.lastname: Clark
authors.forename: Wan
authors.initials: W
authors.lastname: Kuang
authors.forename: Jeunghoon
authors.initials: J
authors.lastname: Lee
authors.forename: Cheryl
authors.initials: C
authors.lastname: Jorcyk
authors.forename: William B
authors.initials: WB
authors.lastname: Knowlton
authors.forename: Bernard
authors.initials: B
authors.lastname: Yurke
authors.forename: William L
authors.initials: WL
authors.lastname: Hughes
authors.forename: Elton
authors.initials: E
authors.lastname: Graugnard
mesh_terms: D039301:Computers, Molecular; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure*; D006801:Humans; D036103:Nanotechnology / Q000379:methods; D009690:Nucleic Acid Conformation; D044967:Serum / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D004247:DNA