title: Association of tumor necrosis factor-α promoter G-308A gene polymorphism with increased triglyceride level of subjects with metabolic syndrome.
abstract: BACKGROUND
  The G-308A single nucleotide polymorphism (SNP) in the promoter of tumor necrosis factor-alpha (TNF-α) gene has previously been reported to be associated with cardiovascular risk. However the potential association of this polymorphism with metabolic syndrome (MetS) is unclear. The aim of the current study was to explore the association of this TNF-α gene polymorphism with MetS in an Iranian population.
  
  
  METHODS
  Two hundred and twenty two subjects were recruited and anthropometric and biochemical parameters were determined. Genotyping was performed using the amplification refractory mutation system polymerase chain reaction. The association of the genetic-polymorphism with MetS was evaluated by univariate and multivariate analyses.
  
  
  RESULTS
  MetS subjects had a significantly (P<0.05) higher level of fasted serum triglycerides, body-mass-index, waist-circumference, blood pressure and fasting-blood-glucose, and lower level of high-density lipoprotein cholesterol. The possession of AA or GA genotype of the TNF-α gene was not associated with MetS in our population. However the AA genotype of TNF-α was related to an increased level of triglyceride in MetS patients, compared to the control group.
  
  
  CONCLUSIONS
  TNF-α G-308A polymorphism is unlikely to play an important role in the development of MetS in our population.
authors.affiliation: Department of Basic Medical Sciences, Neyshabur University of Medical Sciences, Neyshabur, Iran; Department of New Science and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Seyed Reza
authors.initials: SR
authors.lastname: Mirhafez
authors.affiliation: Department of New Science and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; Biochemistry of Nutrition Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Amir
authors.initials: A
authors.lastname: Avan
authors.affiliation: Department of New Science and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; Division of Applied Medicine, Medical School, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK.
authors.forename: Alireza
authors.initials: A
authors.lastname: Pasdar
authors.affiliation: Department of Biochemistry, International Campus of Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: Elaheh
authors.initials: E
authors.lastname: Kazemi
authors.affiliation: Department of New Science and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Faezeh
authors.initials: F
authors.lastname: Ghasemi
authors.affiliation: Department of New Science and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Amir
authors.initials: A
authors.lastname: Tajbakhsh
authors.affiliation: Department of Basic Medical Sciences, Neyshabur University of Medical Sciences, Neyshabur, Iran.
authors.forename: Samaneh
authors.initials: S
authors.lastname: Tabaee
authors.affiliation: Brighton & Sussex Medical School, Division of Medical Education, Falmer, Brighton, Sussex BN1 9PH, UK.
authors.forename: Gordon A
authors.initials: GA
authors.lastname: Ferns
authors.affiliation: Biochemistry of Nutrition Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: ghayourm@mums.ac.ir.
authors.forename: Majid
authors.initials: M
authors.lastname: Ghayour-Mobarhan
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D024821:Metabolic Syndrome / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D014280:Triglycerides / Q000097:blood*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: C439524:TNF protein, human; D014280:Triglycerides; D014409:Tumor Necrosis Factor-alpha
keywords: G-308A polymorphism; Metabolic syndrome; Tumor necrosis factor-alpha