title: Incorporation of Unnatural Amino Acids in Response to the AGG Codon.
abstract: The biological protein synthesis system has been engineered to incorporate unnatural amino acid into proteins, and this has opened up new routes for engineering proteins with novel compositions. While such systems have been successfully applied in research, there remains a need to develop new approaches with respect to the wider application of unnatural amino acids. In this study, we reported a strategy for incorporating unnatural amino acids into proteins by reassigning one of the Arg sense codons, the AGG codon. Using this method, several unnatural amino acids were quantitatively incorporated into the AGG site. Furthermore, we applied the method to multiple AGG sites, and even to tandem AGG sequences. The method developed and described here could be used for engineering proteins with diverse unnatural amino acids, particularly when employed in combination with other methods.
authors.forename: Byeong Sung
authors.initials: BS
authors.lastname: Lee
authors.forename: Seunggun
authors.initials: S
authors.lastname: Shin
authors.forename: Jong Yeob
authors.initials: JY
authors.lastname: Jeon
authors.affiliation: §Division of Mass Spectrometry Research, Korea Basic Science Institute, 162 Yeongudanji-ro, Ochang-eup, Cheongwon-gu, Cheongju 363-883, Korea.
authors.forename: Kyoung-Soon
authors.initials: KS
authors.lastname: Jang
authors.forename: Bun Yeol
authors.initials: BY
authors.lastname: Lee
authors.forename: Sangdun
authors.initials: S
authors.lastname: Choi
authors.forename: Tae Hyeon
authors.initials: TH
authors.lastname: Yoo
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D003062:Codon; D004926:Escherichia coli / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D014176:Protein Biosynthesis; D015202:Protein Engineering / Q000379:methods*
chemical_list: D000596:Amino Acids; D003062:Codon