title: Interaction of cellular proteins with BCL-xL targeted to cytoplasmic inclusion bodies in adenovirus infected cells.
abstract: Adenovirus-mediated apoptosis was suppressed when cellular anti-apoptosis proteins (BCL-2 and BCL-xL) were substituted for the viral E1B-19K. For unbiased proteomic analysis of proteins targeted by BCL-xL in adenovirus-infected cells and to visualize the interactions with target proteins, BCL-xL was targeted to cytosolic inclusion bodies utilizing the orthoreovirus µNS protein sequences. The chimeric protein was localized in non-canonical cytosolic factory-like sites and promoted survival of virus-infected cells. The BCL-xL-associated proteins were isolated from the cytosolic inclusion bodies in adenovirus-infected cells and analyzed by LC-MS. These proteins included BAX, BAK, BID, BIK and BIM as well as mitochondrial proteins such as prohibitin 2, ATP synthase and DNA-PKcs. Our studies suggested that in addition to the interaction with various pro-apoptotic proteins, the association with certain mitochondrial proteins such as DNA-PKcs and prohibitins might augment the survival function of BCL-xL in virus infected cells.
authors.affiliation: Institute for Molecular Virology, Saint Louis University Health Sciences Center, Doisy Research Center, 1100 South Grand Blvd, Saint Louis, MO 63104, USA.
authors.forename: T
authors.initials: T
authors.lastname: Subramanian
authors.affiliation: Institute for Molecular Virology, Saint Louis University Health Sciences Center, Doisy Research Center, 1100 South Grand Blvd, Saint Louis, MO 63104, USA.
authors.forename: S
authors.initials: S
authors.lastname: Vijayalingam
authors.affiliation: Institute for Molecular Virology, Saint Louis University Health Sciences Center, Doisy Research Center, 1100 South Grand Blvd, Saint Louis, MO 63104, USA.
authors.forename: M
authors.initials: M
authors.lastname: Kuppuswamy
authors.affiliation: Institute for Molecular Virology, Saint Louis University Health Sciences Center, Doisy Research Center, 1100 South Grand Blvd, Saint Louis, MO 63104, USA. Electronic address: chinnag@slu.edu.
authors.forename: G
authors.initials: G
authors.lastname: Chinnadurai
mesh_terms: D000256:Adenoviridae / Q000254:growth & development*; D017209:Apoptosis; D002460:Cell Line; D002470:Cell Survival; D002853:Chromatography, Liquid; D006801:Humans; D002479:Inclusion Bodies / Q000821:virology*; D013058:Mass Spectrometry; D024101:Mitochondrial Proteins / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D051020:bcl-X Protein / Q000378:metabolism*
chemical_list: D024101:Mitochondrial Proteins; D051020:bcl-X Protein
keywords: Adenovirus; Apoptosis; BCL-xL; E1B-19K; Interacting proteins; Orthoreovirus µNS