title: Quality evaluation and pattern recognition analyses of bioactive marker compounds from Farfarae Flos using HPLC/PDA.
abstract: The flower bud of Tussilago farfara L., called Farfarae Flos, has traditionally been used in Oriental medicine for the treatment of bronchitis and asthma. To establish a standard for quality control as well as the reliable identification of Farfarae Flos, the contents of five standards, rutin (1), isoquercetin (2), 3,5-dicaffeoylquinic acid (3), tussilagone (4), and tussilagonone (5), were determined by quantitative high-performance liquid chromatography (HPLC)/photodiode array (PDA) analysis. The five standards were separated on a YoungJinBioChrom Aegispak C18-L (250-mm×4.6-mm, 5-µm) column by gradient elution using 0.03% trifluoroacetic acid in water (A), with acetonitrile (B) as the mobile phase. The flow rate was 1.0 mL/min, and the UV detector wavelength was set at 220 nm. The method was successfully used in the analysis of Farfarae Flos from different geographic origins with relatively simple conditions and procedures, and the results demonstrated satisfactory linearity, recovery, precision, accuracy, stability, and robustness. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of 62 Farfarae Flos samples. This result indicated that the established HPLC/PDA method is suitable for quantitation and pattern recognition analyses for the quality evaluation of Farfarae Flos.
authors.affiliation: College of Pharmacy, Catholic University of Daegu.
authors.forename: U Min
authors.initials: UM
authors.lastname: Seo
authors.forename: Bing Tian
authors.initials: BT
authors.lastname: Zhao
authors.forename: Won Il
authors.initials: WI
authors.lastname: Kim
authors.forename: Eun Kyoung
authors.initials: EK
authors.lastname: Seo
authors.forename: Jae Hyun
authors.initials: JH
authors.lastname: Lee
authors.forename: Byung Sun
authors.initials: BS
authors.lastname: Min
authors.forename: Beom Soo
authors.initials: BS
authors.lastname: Shin
authors.forename: Jong Keun
authors.initials: JK
authors.lastname: Son
authors.forename: Mi Hee
authors.initials: MH
authors.lastname: Woo
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000592:standards; D016000:Cluster Analysis; D035264:Flowers / Q000737:chemistry / Q000378:metabolism; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D011786:Quality Control; D031207:Tussilago / Q000737:chemistry* / Q000378:metabolism
chemical_list: D010936:Plant Extracts