title: MicroRNA-21 promotes proliferation of rat hepatocyte BRL-3A by targeting FASLG.
abstract: Rat liver regeneration (RLR) induced by partial hepatectomy involves cell proliferation regulated by numerous factors, including microRNAs (miRNAs). miRNA high-throughput sequencing has been established and used to analyze miRNA expression profiles. This study showed that 39 miRNAs were related to RLR through the analysis of miRNA high-throughput sequencing. Their role toward rat normal hepatocyte line BRL-3A was studied by gain- and loss-of-function analyses, and one of them, microRNA-21 (miR-21), obviously upregulated and promoted BRL-3A cell proliferation. Using bioinformatics to search for miR-21 targets revealed that Fas ligand (FASLG) is one of miR-21's target genes. A dual-luciferase report assay and Western blot assay showed that miR-21 directly targeted the 3'-untranslated region of FASLG and inhibited the expression of FASLG, which suggests that miR-21 promoted BRL-3A cell proliferation by reducing FASLG expression.
authors.affiliation: State Key Laboratory Cultivation Base for Cell Differentiation Regulation, Henan Normal University, Xinxiang, China.
authors.forename: J J
authors.initials: JJ
authors.lastname: Li
authors.affiliation: College of Life Science and Technology, Jinan University, Guangzhou, Guangdong Province, China.
authors.forename: W H
authors.initials: WH
authors.lastname: Chan
authors.affiliation: College of Life Science and Technology, Jinan University, Guangzhou, Guangdong Province, China.
authors.forename: W Y
authors.initials: WY
authors.lastname: Leung
authors.affiliation: College of Life Science and Technology, Jinan University, Guangzhou, Guangdong Province, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory Cultivation Base for Cell Differentiation Regulation, Henan Normal University, Xinxiang, China cellkeylab@126.com.
authors.forename: C S
authors.initials: CS
authors.lastname: Xu
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics*; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D001483:Base Sequence; D002460:Cell Line; D049109:Cell Proliferation / Q000235:genetics*; D053222:Fas Ligand Protein / Q000235:genetics* / Q000378:metabolism*; D006498:Hepatectomy; D022781:Hepatocytes / Q000378:metabolism; D059014:High-Throughput Nucleotide Sequencing; D008115:Liver Regeneration / Q000502:physiology; D035683:MicroRNAs / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017422:Sequence Analysis, DNA
chemical_list: D020413:3' Untranslated Regions; D053222:Fas Ligand Protein; C505662:Faslg protein, rat; D035683:MicroRNAs; C546082:mirn21 microRNA, rat