title: Production, purification and crystallization of a trans-sialidase from Trypanosoma vivax.
abstract: Sialidases and trans-sialidases play important roles in the life cycles of various microorganisms. These enzymes can serve nutritional purposes, act as virulence factors or mediate cellular interactions (cell evasion and invasion). In the case of the protozoan parasite Trypanosoma vivax, trans-sialidase activity has been suggested to be involved in infection-associated anaemia, which is the major pathology in the disease nagana. The physiological role of trypanosomal trans-sialidases in host-parasite interaction as well as their structures remain obscure. Here, the production, purification and crystallization of a recombinant version of T. vivax trans-sialidase 1 (rTvTS1) are described. The obtained rTvTS1 crystals diffracted to a resolution of 2.5 Å and belonged to the orthorhombic space group P212121, with unit-cell parameters a = 57.3, b = 78.4, c = 209.0 Å.
authors.affiliation: Structural Biology Research Center (SBRC), VIB, Pleinlaan 2, B-1050 Brussels, Belgium.
authors.forename: Carole L F
authors.initials: CL
authors.lastname: Haynes
authors.affiliation: Department for Molecular Biomedical Research (DMBR), UGent, Ghent, Belgium.
authors.forename: Paul
authors.initials: P
authors.lastname: Ameloot
authors.affiliation: Structural Biology Research Center (SBRC), VIB, Pleinlaan 2, B-1050 Brussels, Belgium.
authors.forename: Han
authors.initials: H
authors.lastname: Remaut
authors.affiliation: Department for Molecular Biomedical Research (DMBR), UGent, Ghent, Belgium.
authors.forename: Nico
authors.initials: N
authors.lastname: Callewaert
authors.affiliation: Structural Biology Research Center (SBRC), VIB, Pleinlaan 2, B-1050 Brussels, Belgium.
authors.forename: Yann G J
authors.initials: YG
authors.lastname: Sterckx
authors.affiliation: Structural Biology Research Center (SBRC), VIB, Pleinlaan 2, B-1050 Brussels, Belgium.
authors.forename: Stefan
authors.initials: S
authors.lastname: Magez
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D006023:Glycoproteins / Q000737:chemistry* / Q000302:isolation & purification*; D009439:Neuraminidase / Q000737:chemistry* / Q000302:isolation & purification*; D016689:Trypanosoma vivax / Q000737:chemistry* / Q000201:enzymology*
chemical_list: D006023:Glycoproteins; C069715:trans-sialidase; D009439:Neuraminidase
keywords: Trypanosoma vivax; trans-sialidase