title: Direct adventitious shoot bud formation on hypocotyls explants in Millettia pinnata (L.) Panigrahi- a biodiesel producing medicinal tree species.
abstract: A reproducible protocol developed for in vitro regeneration of Milletia pinnata using hypocotyl segments. Multiple shoots were induced from hypocotyl explants through direct adventitious shoot bud regeneration. The proximal end of hypocotyls was responsive for shoot bud induction. Silver nitrate and adenine sulphate had a positive effect on shoot bud induction and elongation. The maximum response and number of shoot bud produced in media supplemented with 8.88 μM BAP with 108.6 μM adenine sulphate and 11.84 μM silver nitrate. Elongated shoots were harvested and successful rooting of microshoots achieved on MS media supplemented with 9.84 μM IBA, with 81.1 % rooting. Remaining shoot buds sub-cultured for further multiplication and elongation. Each subculture produced eight to nine elongated microshoots up to four subcultures. The rooted microshoots were successfully hardened and transferred to field.
authors.affiliation: Department of Forest Biology and Tree Improvement, College of Forestry, Navsari Agricultural University, Navsari, Gujarat 396 450 India.
authors.forename: Durga Singh
authors.initials: DS
authors.lastname: Nagar
authors.affiliation: Department of Forest Biology and Tree Improvement, College of Forestry, Navsari Agricultural University, Navsari, Gujarat 396 450 India.
authors.forename: Suman Kumar
authors.identifier: 0000-0001-6030-6771
authors.initials: SK
authors.lastname: Jha
authors.affiliation: Department of Forest Biology and Tree Improvement, College of Forestry, Navsari Agricultural University, Navsari, Gujarat 396 450 India.
authors.forename: Jigar
authors.initials: J
authors.lastname: Jani
keywords: Adenine sulphate; Direct adventitious bud; Hypocotyl; In vitro; Milletia pinnata; Silver nitrate