title: A Microfluidic Platform for Evaluating Neutrophil Chemotaxis Induced by Sputum from COPD Patients.
abstract: Chronic Obstructive Pulmonary Disease (COPD) is a common lung disease characterized by breathing difficulty as a consequence of narrowed airways. Previous studies have shown that COPD is correlated with neutrophil infiltration into the airways through chemotactic migration. However, whether neutrophil chemotaxis can be used to characterize and diagnose COPD is not well established. In the present study, we developed a microfluidic platform for evaluating neutrophil chemotaxis to sputum samples from COPD patients. Our results show increased neutrophil chemotaxis to COPD sputum compared to control sputum from healthy individuals. The level of COPD sputum induced neutrophil chemotaxis was correlated with the patient's spirometry data. The cell morphology of neutrophils in a COPD sputum gradient is similar to the morphology displayed by neutrophils exposed to an IL-8 gradient, but not a fMLP gradient. In competing gradients of COPD sputum and fMLP, neutrophils chemotaxis and cell morphology are dominated by fMLP.
authors.affiliation: Department of Physics and Astronomy, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada; Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada.
authors.forename: Jiandong
authors.initials: J
authors.lastname: Wu
authors.affiliation: Seven Oaks General Hospital, Winnipeg, MB, R2V 3M3, Canada.
authors.forename: Craig
authors.initials: C
authors.lastname: Hillier
authors.affiliation: Seven Oaks General Hospital, Winnipeg, MB, R2V 3M3, Canada.
authors.forename: Paul
authors.initials: P
authors.lastname: Komenda
authors.affiliation: Seven Oaks General Hospital, Winnipeg, MB, R2V 3M3, Canada.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Lobato de Faria
authors.affiliation: Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada.
authors.forename: David
authors.initials: D
authors.lastname: Levin
authors.affiliation: Seven Oaks General Hospital, Winnipeg, MB, R2V 3M3, Canada; Rizhao Hospital of Traditional Chinese Medicine, Rizhao, China.
authors.forename: Michael
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Physics and Astronomy, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada; Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada; Department of Biological Sciences, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada; Department of Immunology, University of Manitoba, Winnipeg, MB, R3E 0T5, Canada.
authors.forename: Francis
authors.initials: F
authors.lastname: Lin
mesh_terms: D000328:Adult; D000368:Aged; D002634:Chemotaxis, Leukocyte / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000166:cytology / Q000502:physiology; D029424:Pulmonary Disease, Chronic Obstructive / Q000378:metabolism / Q000503:physiopathology*; D013183:Sputum / Q000737:chemistry*; D055815:Young Adult
chemical_list: D016209:Interleukin-8