title: Exploiting SNPs for biallelic CRISPR mutations in the outcrossing woody perennial Populus reveals 4-coumarate:CoA ligase specificity and redundancy.
authors.affiliation: School of Forestry and Natural Resources, University of Georgia, Athens, GA, 30602, USA.|Department of Genetics, University of Georgia, Athens, GA, 30602, USA.|College of Forest Resources and Environment, Nanjing Forestry University, Nanjing, 210037, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Institute for Plant Breeding, Genetics and Genomics, University of Georgia, Athens, GA, 30602, USA.|Center for Applied Genetic Technologies, University of Georgia, Athens, GA, 30602, USA.
authors.forename: Thomas B
authors.initials: TB
authors.lastname: Jacobs
authors.affiliation: School of Forestry and Natural Resources, University of Georgia, Athens, GA, 30602, USA.|Department of Genetics, University of Georgia, Athens, GA, 30602, USA.
authors.forename: Liang-Jiao
authors.initials: LJ
authors.lastname: Xue
authors.affiliation: School of Forestry and Natural Resources, University of Georgia, Athens, GA, 30602, USA.|Department of Genetics, University of Georgia, Athens, GA, 30602, USA.
authors.forename: Scott A
authors.initials: SA
authors.lastname: Harding
authors.affiliation: School of Forestry and Natural Resources, University of Georgia, Athens, GA, 30602, USA.|Department of Genetics, University of Georgia, Athens, GA, 30602, USA.
authors.forename: Chung-Jui
authors.initials: CJ
authors.lastname: Tsai
mesh_terms: D000483:Alleles; D001483:Base Sequence; D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics*; D003066:Coenzyme A Ligases / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D032107:Populus / Q000201:enzymology / Q000235:genetics*; D013379:Substrate Specificity; D014934:Wood / Q000235:genetics*
chemical_list: D003066:Coenzyme A Ligases; C019943:4-coumarate-CoA ligase
keywords: 4-coumarate:CoA ligase (4CL); Populus; chlorogenic acid; flavonoid; genome editing; lignin; phenylpropanoid