title: Quantification of CD95-induced apoptosis and NF-κB activation at the single cell level.
abstract: CD95/Fas/APO-1 is a member of the death receptor (DR) family. Stimulation of CD95 leads to the induction of apoptosis as well as to NF-κB signaling. Crosstalk between these two pathways plays a central role in cell fate. Defects in the regulation of apoptosis and of NF-κB are connected to a number of chronic inflammatory diseases and cancer. For a better understanding of the life/death decisions in the cell and their contribution to disease progression, the development of new technologies is required. Using imaging flow cytometry we developed a method that enables a quantitative detection of different CD95 signaling pathways in the single cell. The important advantage of this method compared to other approaches is that it allows quantifying a large number of single cells undergoing apoptosis and NF-κB activation. This technology could provide new insights into the quantitative characterization of apoptosis and NF-κB at the single cell level and could be used for the quantitative network analysis in systems biology studies.
authors.affiliation: Department of Translational Inflammation Research, Institute of Experimental Internal Medicine, Otto von Guericke University, Pfälzer Platz, 39106 Magdeburg, Germany. Electronic address: joern.schmidt@med.ovgu.de.
authors.forename: Jörn H
authors.initials: JH
authors.lastname: Schmidt
authors.affiliation: Department of Translational Inflammation Research, Institute of Experimental Internal Medicine, Otto von Guericke University, Pfälzer Platz, 39106 Magdeburg, Germany. Electronic address: sabine.pietkiewicz@med.ovgu.de.
authors.forename: Sabine
authors.initials: S
authors.lastname: Pietkiewicz
authors.affiliation: Institute of Experimental Internal Medicine, Otto von Guericke University, Leipziger Straße 44, 39120 Magdeburg, Germany. Electronic address: naumann@med.ovgu.de.
authors.forename: Michael
authors.initials: M
authors.lastname: Naumann
authors.affiliation: Department of Translational Inflammation Research, Institute of Experimental Internal Medicine, Otto von Guericke University, Pfälzer Platz, 39106 Magdeburg, Germany. Electronic address: inna.lavrik@med.ovgu.de.
authors.forename: Inna N
authors.initials: IN
authors.lastname: Lavrik
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D045744:Cell Line, Tumor; D005434:Flow Cytometry / Q000379:methods; D006367:HeLa Cells; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism*; D059010:Single-Cell Analysis / Q000379:methods; D049490:Systems Biology / Q000379:methods; D019014:fas Receptor / Q000378:metabolism*
chemical_list: C491901:FAS protein, human; D016328:NF-kappa B; D019014:fas Receptor
keywords: Apoptosis; CD95/Fas; Imaging flow cytometry; NF-κB; Single cells analysis