title: Supramolecular Assembly and Coalescence of Ferritin Cages Driven by Designed Protein-Protein Interactions.
abstract: A genetically encoded system for expression of supramolecular protein assemblies (SMPAs) based on a fusion construct between ferritin and citrine (YFP) was transferred from a mammalian to a bacterial host. The assembly process is revealed to be independent of the expression host, while dimensions and level of order of the assembled structures were influenced by the host organism. An additional level of interactions, namely, coalescence between the preformed SMPAs, was observed during the purification process. SAXS investigation revealed that upon coalescence, the local order of the individual SMPAs was preserved. Finally, the chaotropic agent urea effectively disrupted both the macroscopic coalescence and the interactions at the nanoscale until the level of the single ferritin cage.
authors.affiliation: †Department of Materials and Interfaces, Weizmann Institute of Science, 76100 Rehovot, Israel.
authors.forename: Giuliano
authors.initials: G
authors.lastname: Bellapadrona
authors.affiliation: ‡Indian Institute of Technology, Bombay, Mumbai Area 400076, India.
authors.forename: Shwetali
authors.initials: S
authors.lastname: Sinkar
authors.affiliation: §Department of Chemical Research Support, Weizmann Institute of Science, 76100 Rehovot, Israel.
authors.forename: Helena
authors.initials: H
authors.lastname: Sabanay
authors.affiliation: ∥Biohybrid Materials Group, Department of Biotechnology and Chemical Technology, Aalto University, 00076 Aalto, Finland.
authors.forename: Ville
authors.initials: V
authors.lastname: Liljeström
authors.affiliation: ∥Biohybrid Materials Group, Department of Biotechnology and Chemical Technology, Aalto University, 00076 Aalto, Finland.
authors.forename: Mauri
authors.initials: M
authors.lastname: Kostiainen
authors.affiliation: †Department of Materials and Interfaces, Weizmann Institute of Science, 76100 Rehovot, Israel.
authors.forename: Michael
authors.initials: M
authors.lastname: Elbaum
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005293:Ferritins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D046911:Macromolecular Substances / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D060066:Protein Interaction Maps; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D053838:Scattering, Small Angle; D014961:X-Ray Diffraction
chemical_list: D001426:Bacterial Proteins; D008164:Luminescent Proteins; D046911:Macromolecular Substances; D011993:Recombinant Fusion Proteins; C434479:citrine protein, bacteria; D005293:Ferritins