title: Structures of a histidine triad family protein from Entamoeba histolytica bound to sulfate, AMP and GMP.
abstract: Three structures of the histidine triad family protein from Entamoeba histolytica, the causative agent of amoebic dysentery, were solved at high resolution within the Seattle Structural Genomics Center for Infectious Disease (SSGCID). The structures have sulfate (PDB entry 3oj7), AMP (PDB entry 3omf) or GMP (PDB entry 3oxk) bound in the active site, with sulfate occupying the same space as the α-phosphate of the two nucleotides. The C(α) backbones of the three structures are nearly superimposable, with pairwise r.m.s.d.s ranging from 0.06 to 0.13 Å.
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease, USA.
authors.forename: Donald D
authors.initials: DD
authors.lastname: Lorimer
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease, USA.
authors.forename: Ryan
authors.initials: R
authors.lastname: Choi
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease, USA.
authors.forename: Ariel
authors.initials: A
authors.lastname: Abramov
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease, USA.
authors.forename: Stephen
authors.initials: S
authors.lastname: Nakazawa Hewitt
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease, USA.
authors.forename: Anna S
authors.initials: AS
authors.lastname: Gardberg
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease, USA.
authors.forename: Wesley C
authors.initials: WC
authors.lastname: Van Voorhis
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease, USA.
authors.forename: Bart L
authors.initials: BL
authors.lastname: Staker
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease, USA.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Myler
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease, USA.
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Edwards
mesh_terms: D000249:Adenosine Monophosphate / Q000378:metabolism*; D000595:Amino Acid Sequence; D003460:Crystallization; D004748:Entamoeba histolytica / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006157:Guanosine Monophosphate / Q000378:metabolism*; D006867:Hydrolases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D011485:Protein Binding / Q000502:physiology; D017433:Protein Structure, Secondary; D013431:Sulfates / Q000378:metabolism*
chemical_list: D013431:Sulfates; C097552:histidine triad protein; D000249:Adenosine Monophosphate; D006157:Guanosine Monophosphate; D006867:Hydrolases
keywords: SSGCID; Seattle Structural Genomics Center for Infectious Disease; hydrolase; metal-binding protein; structural genomics