title: Polyurethane/polylactide-based biomaterials combined with rat olfactory bulb-derived glial cells and adipose-derived mesenchymal stromal cells for neural regenerative medicine applications.
abstract: Research concerning the elaboration and application of biomaterial which may support the nerve tissue regeneration is currently one of the most promising directions. Biocompatible polymer devices are noteworthy group among the numerous types of potentially attractive biomaterials for regenerative medicine application. Polylactides and polyurethanes may be utilized for developing devices for supporting the nerve regeneration, like nerve guide conduits or bridges connecting the endings of broken nerve tracts. Moreover, the combination of these biomaterial devices with regenerative cell populations, like stem or precursor cells should significantly improve the final therapeutic effect. Therefore, the composition and structure of final device should support the proper adhesion and growth of cells destined for clinical application. In current research, the three polymer mats elaborated for connecting the broken nerve tracts, made from polylactide, polyurethane and their blend were evaluated both for physical properties and in vitro, using the olfactory-bulb glial cells and mesenchymal stem cells. The evaluation of Young's modulus, wettability and roughness of obtained materials showed the differences between analyzed samples. The analysis of cell adhesion, proliferation and morphology showed that the polyurethane-polylactide blend was the most neutral for cells in culture, while in the pure polymer samples there were significant alterations observed. Our results indicated that polyurethane-polylactide blend is an optimal composition for culturing and delivery of glial and mesenchymal stem cells.
authors.affiliation: Electron Microscopy Laboratory, University of Environmental and Life Sciences, Kozuchowska 5b, 51-631 Wroclaw, Poland. Electronic address: grzesiak.kuba@gmail.com.
authors.forename: Jakub
authors.initials: J
authors.lastname: Grzesiak
authors.affiliation: Electron Microscopy Laboratory, University of Environmental and Life Sciences, Kozuchowska 5b, 51-631 Wroclaw, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Marycz
authors.affiliation: Department of Neurosurgery, Lower Silesia Specialist Hospital of T. Marciniak, Emergency Medicine Center, Traugutta 116, 50-420 Wroclaw, Poland.
authors.forename: Dariusz
authors.initials: D
authors.lastname: Szarek
authors.affiliation: State Higher Vocational School in Tarnów, Mickiewicza 8, 33-100 Tarnów, Poland.
authors.forename: Paulina
authors.initials: P
authors.lastname: Bednarz
authors.affiliation: AGH University of Science and Technology, Faculty of Materials Science and Ceramics, Mickiewicza 30, 30-059 Kraków, Poland.
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Laska
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002478:Cells, Cultured; D016130:Immunophenotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects*; D009830:Olfactory Bulb / Q000166:cytology*; D011091:Polyesters / Q000737:chemistry*; D011140:Polyurethanes / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D044968:Regenerative Medicine
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; D011140:Polyurethanes; C033616:poly(lactide)
keywords: Biomaterial; Glial cells; Mesenchymal stem cells; Polylactide; Polyurethane