title: In vivo biological response to highly cross-linked and vitamin e-doped polyethylene--a particle-Induced osteolysis animal study.
abstract: Polyethylene particle-induced osteolysis is the primary limitation in the long-term success of total joint replacement with conventional ultra high molecular weight polyethylene (UHMWPE). Highly cross-linked polyethylene (HXLPE) and vitamin E-doped cross-linked polyethylene (VE-HXLPE) have been developed to increase the wear resistance of joint surfaces. However, very few studies have reported on the incidence of particle-induced osteolysis for these novel materials. The aim of this study was to use a particle-induced osteolysis animal model to compare the in vivo biological response to different polymer particles. Three commercially available polymers (UHMWPE, HXLPE, and VE-HXLPE) were compared. Osseous properties including the bone volume relative to the tissue volume (BV/TV), trabecular thickness (Tb. Th), and bone mineral density (BMD) were examined using micro computed tomography. Histological analysis was used to observe tissue inflammation in each group. This study demonstrated that the osseous properties and noticeable inflammatory reactions were obviously decreased in the HXLPE group. When compared with the sham group, a decrease of 12.7% was found in BV/TV, 9.6% in BMD and 8.3% in Tb.Th for the HXLPE group. The heightened inflammatory response in the HXLPE group could be due to its smaller size and greater amount of implanted particles. Vitamin E diffused in vivo may not affect the inflammatory and osteolytic responses in this model. The morphological size and total cumulative amount of implanted particles could be critical factors in determining the biological response.
authors.affiliation: Biomechanics Research Laboratory, Department of Medical Research, MacKay Memorial Hospital (MMH), Taipei, Taiwan.|Institue of Biomedical Engineering, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Chang-Hung
authors.initials: CH
authors.lastname: Huang
authors.affiliation: Biomechanics Research Laboratory, Department of Medical Research, MacKay Memorial Hospital (MMH), Taipei, Taiwan.|Department of Orthopaedic Surgery, MMH, Taipei, Taiwan.|Department of Cosmetic Application and Management, MacKay Junior College of Medicine, Nursing and Management, Taipei, Taiwan.
authors.forename: Yung-Chang
authors.initials: YC
authors.lastname: Lu
authors.affiliation: Biomechanics Research Laboratory, Department of Medical Research, MacKay Memorial Hospital (MMH), Taipei, Taiwan.|Institue of Biomedical Engineering, National Yang-Ming University, Taipei, Taiwan.|Department of Orthopaedic Surgery, MMH, Taipei, Taiwan.
authors.forename: Ting-Kuo
authors.initials: TK
authors.lastname: Chang
authors.affiliation: Biomechanics Research Laboratory, Department of Medical Research, MacKay Memorial Hospital (MMH), Taipei, Taiwan.
authors.forename: I-Lin
authors.initials: IL
authors.lastname: Hsiao
authors.affiliation: Department of Chemical Engineering and Biotechnology and Institute of Biotechnology, National Taipei University of Technology, Taipei, Taiwan.
authors.forename: Yi-Ching
authors.initials: YC
authors.lastname: Su
authors.affiliation: Biomechanics Research Laboratory, Department of Medical Research, MacKay Memorial Hospital (MMH), Taipei, Taiwan.
authors.forename: Shu-Ting
authors.initials: ST
authors.lastname: Yeh
authors.affiliation: Department of Chemical Engineering and Biotechnology and Institute of Biotechnology, National Taipei University of Technology, Taipei, Taiwan.
authors.forename: Hsu-Wei
authors.initials: HW
authors.lastname: Fang
authors.affiliation: Institue of Biomedical Engineering, National Yang-Ming University, Taipei, Taiwan.|Department of Orthopaedic Surgery, MMH, Taipei, Taiwan.
authors.forename: Chun-Hsiung
authors.initials: CH
authors.lastname: Huang
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D051379:Mice; D010014:Osteolysis / Q000139:chemically induced* / Q000378:metabolism* / Q000473:pathology*; D020959:Polyethylene / Q000009:adverse effects* / Q000737:chemistry / Q000494:pharmacology; D014810:Vitamin E / Q000009:adverse effects* / Q000737:chemistry / Q000494:pharmacology
chemical_list: D014810:Vitamin E; D020959:Polyethylene
keywords: antioxidant; cross-linked; osteolysis; particulate debris; polyethylene