title: Effects of Ganoderma lucidum (Higher Basidiomycetes) Extracts on the miRNA Profile and Telomerase Activity of the MCF-7 Breast Cancer Cell Line.
abstract: Ganoderma lucidum is a medicinal higher Basidiomycetes mushroom that exerts anticancer effects through several different mechanisms. This study investigated the effects of G. lucidum on the telomerase activity and microRNA (miRNA) profiles of MCF-7 cells. According to the cytotoxicity results, the G. lucidum ether extract exhibits the highest cytotoxic potency; therefore it was chosen for the subsequent telomerase activity assay and miRNA profiling. The telomerase activity observed in the cells treated with a half-maximal inhibitory concentration of G. lucidum ether extract (100 µg/mL in dimethyl sulfoxide) was 32.2% lower than that of the control cells treated with 1% dimethyl sulfoxide. Among 1066 miRNAs, the most downregulated miRNA was hsa-miR-27a* (4.469-fold), and the most upregulated miRNA was hsa-miR-1285 (10.462-fold). A database search revealed the predicted miRNAs that target the catalytic subunit of the telomerase enzyme telomerase reverse transcriptase, and only miR-3687 (upregulated 2.153-fold) and miR-1207-5p (upregulated 2.895-fold) were changed by at least 2-fold. The miRNA profile changes demonstrated in this study provide a data set regarding their effects on the pathways that regulate telomerase activity in MCF-7 breast cancer cells treated with G. lucidum. These data should aid the development of novel cancer treatment strategies.
authors.affiliation: Department of Medical Biochemistry, Ege University, Bornova, Izmir, Turkey.
authors.forename: Oyku
authors.initials: O
authors.lastname: Gonul
authors.affiliation: Department of Medical Biochemistry, Ege University, Bornova, Izmir, Turkey.
authors.forename: Hikmet Hakan
authors.initials: HH
authors.lastname: Aydin
authors.affiliation: Ministry of Science, Industry and Technology, Manisa, Turkey.
authors.forename: Erbil
authors.initials: E
authors.lastname: Kalmis
authors.affiliation: Department of Pharmacognosy, Ege University, Bornova, Izmir, Turkey.
authors.forename: Husniye
authors.initials: H
authors.lastname: Kayalar
authors.affiliation: Department of Medical Biochemistry, Ege University, Bornova, Izmir, Turkey.
authors.forename: Ali Burak
authors.initials: AB
authors.lastname: Ozkaya
authors.affiliation: Department of Medical Biochemistry, Ege University, Bornova, Izmir, Turkey.
authors.forename: Sevcan
authors.initials: S
authors.lastname: Atay
authors.affiliation: Department of Medical Biochemistry, Ege University, Bornova, Izmir, Turkey.
authors.forename: Handan
authors.initials: H
authors.lastname: Ak
mesh_terms: D001943:Breast Neoplasms / Q000188:drug therapy / Q000201:enzymology* / Q000235:genetics* / Q000503:physiopathology; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D020940:Reishi / Q000737:chemistry*; D019098:Telomerase / Q000235:genetics / Q000378:metabolism*; D059467:Transcriptome / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D004365:Drugs, Chinese Herbal; D035683:MicroRNAs; D019098:Telomerase