title: MicroRNA-153 functions as a tumor suppressor by targeting SET7 and ZEB2 in ovarian cancer cells.
abstract: The overall goal of the present study was to find and validate unidentified miRNAs that regulate epithelial-mesenchymal transition (EMT) and proliferation in ovarian cancer. Furthermore, we demonstrate that the high expression of miR-153 in human epithelial ovarian cancer (EOC) is associated with better survival. The mean expression level of miR-153 in ovarian cancer was significantly lower than in the adjacent carcinoma tissue. In the present study, we report that miR-153 are negative regulators of SET7 and ZEB2, miR-153 regulates SET7/ZEB2 expression and promotes SET7/ZEB2 mRNA degradation. Further, confirmed by reporter assays, SET7/ZEB2 are downstream targets of miR-153 directly bound to the 3' untranslated region (3'-UTR). Clone formation and wound-healing assay as well as Transwell assay proved that silencing of SET7 or ZEB2 partially abolished the enhancement of cell proliferation and invasion induced by downregulated miR-153. SET7 and ZEB2 are negatively correlated with miR-153 expression in human ovarian cancer and indicated a worse survival. Considering the role of SET7 and ZEB2 in EOC, it is important to clarify how the expression of SET7 and ZEB2 are regulated. Based on our results miR-153 inhibits proliferation and suppresses EMT and the invasive potential of ovarian cancer cells through downregulation of SET7 and ZEB2, supporting the pursuit of miR-153 as a potential target for ovarian cancer intervention.
authors.affiliation: Department of Pathology, School of Basic Medical Science, Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Institute of Pathology Research, Department of Pathology, Xiangnan University, Chenzhou, Hunan 423000, P.R. China.
authors.forename: Ming
authors.initials: M
authors.lastname: Xie
authors.affiliation: Department of Pathology, School of Basic Medical Science, Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Department of Pathology, School of Basic Medical Science, Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Baihua
authors.initials: B
authors.lastname: Luo
authors.affiliation: Department of Pathology, School of Basic Medical Science, Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Guanghui
authors.initials: G
authors.lastname: Gong
authors.affiliation: Department of Pathology, School of Basic Medical Science, Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Juanni
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Pathology, School of Basic Medical Science, Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Junpu
authors.initials: J
authors.lastname: Wang
authors.affiliation: Institute of Pathology Research, Department of Pathology, Xiangnan University, Chenzhou, Hunan 423000, P.R. China.
authors.forename: Wenjian
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Institute of Pathology Research, Department of Pathology, Xiangnan University, Chenzhou, Hunan 423000, P.R. China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zi
authors.affiliation: Department of Pathology, School of Basic Medical Science, Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Pathology, School of Basic Medical Science, Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Jifang
authors.initials: J
authors.lastname: Wen
mesh_terms: D020413:3' Untranslated Regions; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D011495:Histone-Lysine N-Methyltransferase / Q000096:biosynthesis* / Q000235:genetics; D018398:Homeodomain Proteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology; D012097:Repressor Proteins / Q000096:biosynthesis* / Q000235:genetics; D000074165:Zinc Finger E-box Binding Homeobox 2
chemical_list: D020413:3' Untranslated Regions; D018398:Homeodomain Proteins; C556500:MIRN153 microRNA, human; D035683:MicroRNAs; D012097:Repressor Proteins; C492363:ZEB2 protein, human; D000074165:Zinc Finger E-box Binding Homeobox 2; D011495:Histone-Lysine N-Methyltransferase; C540312:SETD7 protein, human