title: Qualitative and Quantitative Analysis of Lignan Constituents in Caulis Trachelospermi by HPLC-QTOF-MS and HPLC-UV.
abstract: A high-performance liquid chromatography coupled with quadrupole tandem time-of-flight mass (HPLC-QTOF-MS) and ultraviolet spectrometry (HPLC-UV) was established for simultaneous qualitative and quantitative analysis of the major chemical constituents in Caulis Trachelospermi, respectively. The analysis was performed on an Agilent Zorbax Eclipse Plus C18 column (4.6 mm×150 mm, 5 μm) using a binary gradient system of water and methanol, with ultraviolet absorption at 230 nm. Based on high-resolution ESI-MS/MS fragmentation behaviors of the reference standards, the characteristic cleavage patterns of lignano-9, 9'-lactones and lignano-8'-hydroxy-9, 9'-lactones were obtained. The results demonstrated that the characteristic fragmentation patterns are valuable for identifying and differentiating lignano-9,9'-lactones and lignano-8'-hydroxy-9,9'-lactones. As such, a total of 25 compounds in Caulis Trachelospermi were unambiguously or tentatively identified via comparisons with reference standards or literature. In addition, 14 dibenzylbutyrolatone lignans were simultaneously quantified in Caulis Trachelospermi by HPLC-UV method. The method is suitable for the qualitative and quantitative analyses of dibenzylbutyrolatone lignans in Caulis Trachelospermi.
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China. liuxiaoting_2013@163.com.|School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China. liuxiaoting_2013@163.com.
authors.forename: Xiao-Ting
authors.initials: XT
authors.lastname: Liu
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China. xuguang_wang521@sina.com.
authors.forename: Xu-Guang
authors.initials: XG
authors.lastname: Wang
authors.forename: Yu
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China. beijingxurui@sina.com.
authors.forename: Rui
authors.initials: R
authors.lastname: Xu
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China. mengfh331@126.com.
authors.forename: Fan-Hua
authors.initials: FH
authors.lastname: Meng
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China. ynj08@hotmail.com.
authors.forename: Neng-Jiang
authors.initials: NJ
authors.lastname: Yu
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China. zhaoym@nic.bmi.ac.cn.
authors.forename: Yi-Min
authors.initials: YM
authors.lastname: Zhao
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D007783:Lactones / Q000737:chemistry; D017705:Lignans / Q000737:chemistry*; D008516:Medicine, Chinese Traditional / Q000379:methods; D010936:Plant Extracts / Q000737:chemistry*; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D004365:Drugs, Chinese Herbal; D007783:Lactones; D017705:Lignans; D010936:Plant Extracts