title: Using RecA protein to enhance kinetic rates of DNA circuits.
abstract: While DNA circuits are becoming increasingly useful as signal transducers, their utility is inhibited by their slow catalytic rate. Here, we demonstrate how RecA, a recombination enzyme that catalyzes sequence specific strand exchange, can be used to increase circuit rates up to 9-fold. We also show how the introduction of RNA into DNA circuits further controls the specificity of RecA strand exchange, improving signal-to-noise.
authors.affiliation: Institute for Cellular and Molecular Biology, Department of Chemistry and Biochemistry, University of Texas at Austin, 2500 Speedway, Austin, TX, USA. andy.ellington@mail.utexas.edu.
authors.forename: J N
authors.initials: JN
authors.lastname: Milligan
authors.forename: A D
authors.initials: AD
authors.lastname: Ellington
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D002384:Catalysis; D004247:DNA / Q000737:chemistry*; D004926:Escherichia coli; D007700:Kinetics; D012313:RNA / Q000737:chemistry; D011938:Rec A Recombinases / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D012313:RNA; D004247:DNA; D011938:Rec A Recombinases