title: Effect of sulfonylureas administered centrally on the blood glucose level in immobilization stress model.
abstract: Sulfonylureas are widely used as an antidiabetic drug. In the present study, the effects of sulfonylurea administered supraspinally on immobilization stress-induced blood glucose level were studied in ICR mice. Mice were once enforced into immobilization stress for 30 min and returned to the cage. The blood glucose level was measured 30, 60, and 120 min after immobilization stress initiation. We found that intracerebroventricular (i.c.v.) injection with 30 µg of glyburide, glipizide, glimepiride or tolazamide attenuated the increased blood glucose level induced by immobilization stress. Immobilization stress causes an elevation of the blood corticosterone and insulin levels. Sulfonylureas pretreated i.c.v. caused a further elevation of the blood corticosterone level when mice were forced into the stress. In addition, sulfonylureas pretreated i.c.v. alone caused an elevation of the plasma insulin level. Furthermore, immobilization stress-induced insulin level was reduced by i.c.v. pretreated sulfonylureas. Our results suggest that lowering effect of sulfonylureas administered supraspinally against immobilization stress-induced increase of the blood glucose level appears to be primarily mediated via elevation of the plasma insulin level.
authors.affiliation: Department of Pharmacology, Institute of Natural Medicine, Hallym University, Chuncheon 200-702, Korea.
authors.forename: Naveen
authors.initials: N
authors.lastname: Sharma
authors.affiliation: Department of Pharmacology, Institute of Natural Medicine, Hallym University, Chuncheon 200-702, Korea.
authors.forename: Yun-Beom
authors.initials: YB
authors.lastname: Sim
authors.affiliation: Department of Pharmacology, Institute of Natural Medicine, Hallym University, Chuncheon 200-702, Korea.
authors.forename: Soo-Hyun
authors.initials: SH
authors.lastname: Park
authors.affiliation: Department of Pharmacology, Institute of Natural Medicine, Hallym University, Chuncheon 200-702, Korea.
authors.forename: Su-Min
authors.initials: SM
authors.lastname: Lim
authors.affiliation: Department of Pharmacology, Institute of Natural Medicine, Hallym University, Chuncheon 200-702, Korea.
authors.forename: Sung-Su
authors.initials: SS
authors.lastname: Kim
authors.affiliation: Department of Pharmacology, Institute of Natural Medicine, Hallym University, Chuncheon 200-702, Korea.
authors.forename: Jun-Sub
authors.initials: JS
authors.lastname: Jung
authors.affiliation: Department of Physical Education, College of Natural Medicine, College of Medicine, Hallym University, Chuncheon 200-702, Korea.
authors.forename: Jae-Seung
authors.initials: JS
authors.lastname: Hong
authors.affiliation: Department of Pharmacology, Institute of Natural Medicine, Hallym University, Chuncheon 200-702, Korea.
authors.forename: Hong-Won
authors.initials: HW
authors.lastname: Suh
keywords: Blood glucose; Brain; Immobilization stress; Sulfonylurea