title: Heterogeneous cell-cycle behavior in response to UVB irradiation by a population of single cancer cells visualized by time-lapse FUCCI imaging.
abstract: The present study analyzed the heterogeneous cell-cycle dependence and fate of single cancer cells in a population treated with UVB using a fluorescence ubiquitination-based cell-cycle (FUCCI) imaging system. HeLa cells expressing FUCCI were irradiated by 100 or 200 J/m(2) UVB. Modulation of the cell-cycle and apoptosis were observed by time-lapse confocal microscopy imaging every 30 min for 72 h. Correlation between cell survival and factors including cell-cycle phase at the time of the irradiation of UVB, mitosis and the G1/S transition were analyzed using the Kaplan-Meier method along with the log rank test. Time-lapse FUCCI imaging of HeLa cells demonstrated that UVB irradiation induced cell-cycle arrest in S/G2/M phase in the majority of the cells. The cells irradiated by 100 or 200 J/m(2) UVB during G0/G1 phase had a higher survival rate than the cells irradiated during S/G2/M phase. A minority of cells could escape S/G2/M arrest and undergo mitosis which significantly correlated with decreased survival of the cells. In contrast, G1/S transition significantly correlated with increased survival of the cells after UVB irradiation. UVB at 200 J/m(2) resulted in a greater number of apoptotic cells.
authors.affiliation: a AntiCancer, Inc. ; San Diego , CA , USA.
authors.forename: Shinji
authors.initials: S
authors.lastname: Miwa
authors.forename: Shuya
authors.initials: S
authors.lastname: Yano
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kimura
authors.forename: Mako
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Makoto
authors.initials: M
authors.lastname: Toneri
authors.forename: Takashi
authors.initials: T
authors.lastname: Murakami
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Hayashi
authors.forename: Norio
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Toshiyoshi
authors.initials: T
authors.lastname: Fujiwara
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tsuchiya
authors.forename: Robert M
authors.initials: RM
authors.lastname: Hoffman
mesh_terms: D017209:Apoptosis; D002453:Cell Cycle / Q000528:radiation effects*; D002455:Cell Division; D002470:Cell Survival; D002945:Cisplatin / Q000008:administration & dosage; D004317:Doxorubicin / Q000008:administration & dosage; D005456:Fluorescent Dyes / Q000737:chemistry; D016193:G1 Phase; D016195:G2 Phase; D006367:HeLa Cells; D006801:Humans; D053208:Kaplan-Meier Estimate; D018613:Microscopy, Confocal; D008938:Mitosis; D016196:S Phase; D054875:Ubiquitination / Q000187:drug effects; D014466:Ultraviolet Rays
chemical_list: D005456:Fluorescent Dyes; D004317:Doxorubicin; D002945:Cisplatin
keywords: FUCCI; HeLa; UVB; apoptosis, cell-cycle; heterogeneity; mitosis, survival analysis; time-lapse imaging