title: Increasing the activity of immobilized enzymes with nanoparticle conjugation.
abstract: The efficiency and selectivity of enzymatic catalysis is useful to a plethora of industrial and manufacturing processes. Many of these processes require the immobilization of enzymes onto surfaces, which has traditionally reduced enzyme activity. However, recent research has shown that the integration of nanoparticles into enzyme carrier schemes has maintained or even enhanced immobilized enzyme performance. The nanoparticle size and surface chemistry as well as the orientation and density of immobilized enzymes all contribute to the enhanced performance of enzyme-nanoparticle conjugates. These improvements are noted in specific nanoparticles including those comprising carbon (e.g., graphene and carbon nanotubes), metal/metal oxides and polymeric nanomaterials, as well as semiconductor nanocrystals or quantum dots.
authors.affiliation: Department of Mechanical Engineering, 2104 Black Engineering, Ames, IA 50011, United States.
authors.forename: Shaowei
authors.initials: S
authors.lastname: Ding
authors.affiliation: Department of Mechanical Engineering, 2104 Black Engineering, Ames, IA 50011, United States.
authors.forename: Allison A
authors.initials: AA
authors.lastname: Cargill
authors.affiliation: Center for Bio/Molecular Science & Engineering, US Naval Research Laboratory, Washington, DC 20375, United States.
authors.forename: Igor L
authors.initials: IL
authors.lastname: Medintz
authors.affiliation: Department of Mechanical Engineering, 2104 Black Engineering, Ames, IA 50011, United States. Electronic address: jcclauss@iastate.edu.
authors.forename: Jonathan C
authors.initials: JC
authors.lastname: Claussen
mesh_terms: D055162:Biocatalysis; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D045663:Quantum Dots
chemical_list: D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D011108:Polymers