title: Comparative fine mapping of the Wax 1 (W1) locus in hexaploid wheat.
abstract: By applying comparative genomics analyses, a high-density genetic linkage map of the Wax 1 ( W1 ) locus was constructed as a framework for map-based cloning. Glaucousness is described as the scattering effect of visible light from wax deposited on the cuticle of plant aerial organs. In wheat, the wax on leaves and stems is mainly controlled by two sets of genes: glaucousness loci (W1 and W2) and non-glaucousness loci (Iw1 and Iw2). Bulked segregant analysis (BSA) and simple sequence repeat (SSR) mapping showed that Wax1 (W1) is located on chromosome arm 2BS between markers Xgwm210 and Xbarc35. By applying comparative genomics analyses, colinearity genomic regions of the W1 locus on wheat 2BS were identified in Brachypodium distachyon chromosome 5, rice chromosome 4 and sorghum chromosome 6, respectively. Four STS markers were developed using the Triticum aestivum cv. Chinese Spring 454 contig sequences and the International Wheat Genome Sequencing Consortium (IWGSC) survey sequences. W1 was mapped into a 0.93 cM genetic interval flanked by markers XWGGC3197 and XWGGC2484, which has synteny with genomic regions of 56.5 kb in Brachypodium, 390 kb in rice and 31.8 kb in sorghum. The fine genetic map can serve as a framework for chromosome landing, physical mapping and map-based cloning of the W1 in wheat.
authors.affiliation: State Key Laboratory for Agrobiotechnology/Department of Plant Genetics and Breeding, China Agricultural University, Beijing, 100193, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Lu
authors.forename: Jinxia
authors.initials: J
authors.lastname: Qin
authors.forename: Guoxin
authors.initials: G
authors.lastname: Wang
authors.forename: Lili
authors.initials: L
authors.lastname: Wang
authors.forename: Zhenzhong
authors.initials: Z
authors.lastname: Wang
authors.forename: Qiuhong
authors.initials: Q
authors.lastname: Wu
authors.forename: Jingzhong
authors.initials: J
authors.lastname: Xie
authors.forename: Yong
authors.initials: Y
authors.lastname: Liang
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: Deyun
authors.initials: D
authors.lastname: Zhang
authors.forename: Qixin
authors.initials: Q
authors.lastname: Sun
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Liu
mesh_terms: D058431:Brachypodium / Q000235:genetics; D001947:Breeding; D032461:Chromosomes, Plant; D055028:Comparative Genomic Hybridization; D018744:DNA, Plant / Q000235:genetics; D020224:Expressed Sequence Tags; D017343:Genes, Plant; D008040:Genetic Linkage; D005819:Genetic Markers; D018895:Microsatellite Repeats; D020161:Physical Chromosome Mapping; D011123:Polyploidy; D014908:Triticum / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers