title: Real-time quantitative reverse transcription-polymerase chain reaction to detect propionibacterial ribosomal RNA in the lymph nodes of Chinese patients with sarcoidosis.
abstract: The aim of this study was to investigate the diagnostic value of using the copy number of propionibacterial rRNA as a biomarker for sarcoidosis. Ribosomal RNA of Propionibacterium acnes and P. granulosum was measured by real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) using formalin-fixed and paraffin-embedded tissue of lymph node biopsy from 65 Chinese patients with sarcoidosis, 45 with tuberculosis and 50 controls with other diseases (23 with non-specific lymphadenitis and 27 with mediastinal lymph node metastasis from lung cancer). The receiver operating characteristic (ROC) curve was analysed to determine an optimal cut-off value for diagnosis, and the diagnostic accuracy of the cut-off value was evaluated in additional tissue samples [24 patients with sarcoidosis and 22 with tuberculosis (TB)]. P. acnes or P. granulosum rRNA was detected in 48 of the 65 sarcoidosis samples but only in four of the 45 TB samples and three of the 50 control samples. Analysis of the ROC curve revealed that an optimal cut-off value of the copy number of propionibacterial rRNA for diagnosis of sarcoidosis was 50·5 copies/ml with a sensitivity and specificity of 73·8 and 92·6%, respectively. Based on the cut-off value, 19 of the 24 additional sarcoidosis samples exhibited positive P. acnes or P. granulosum, whereas only one of the 22 additional TB samples was positive, resulting in a sensitivity and specificity of 79·2 and 95·5%, respectively. These findings suggest that propionibacteria might be associated with sarcoidosis granulomatous inflammation. Detection of propionibacterial rRNA by RT-PCR might possibly distinguish sarcoidosis from TB.
authors.affiliation: Department of Respiratory Medicine, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Respiratory Medicine, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: Y-R
authors.initials: YR
authors.lastname: Wei
authors.affiliation: Department of Respiratory Medicine, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Respiratory Medicine, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: S-S
authors.initials: SS
authors.lastname: Du
authors.affiliation: Department of Medicine, University of Cincinnati Medical Center, Cincinnati, OH, USA.
authors.forename: R P
authors.initials: RP
authors.lastname: Baughman
authors.affiliation: Department of Respiratory Medicine, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: H-P
authors.initials: HP
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D044466:Asian People; D002681:China; D005260:Female; D018628:Gene Dosage; D016908:Gram-Positive Bacterial Infections / Q000175:diagnosis* / Q000208:ethnology / Q000382:microbiology; D054884:Host-Pathogen Interactions; D006801:Humans; D008198:Lymph Nodes / Q000382:microbiology / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D011424:Propionibacterium / Q000145:classification / Q000235:genetics* / Q000502:physiology; D011425:Propionibacterium acnes / Q000235:genetics / Q000502:physiology; D012329:RNA, Bacterial / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics*; D012372:ROC Curve; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012507:Sarcoidosis / Q000175:diagnosis* / Q000208:ethnology / Q000382:microbiology; D014376:Tuberculosis / Q000175:diagnosis / Q000208:ethnology / Q000382:microbiology; D055815:Young Adult
chemical_list: D012329:RNA, Bacterial; D012335:RNA, Ribosomal
keywords: diagnosis; propionibacterium acnes; propionibacterium granulosum; real-time quantitative reverse transcription-polymerase chain reaction; sarcoidosis