title: Effects of bisphenol A on decreasing the percentage and promoting the growth of stem cell-like cells from SK-N-SH human neuroblastoma cells.
abstract: Bisphenol A (BPA) is an industrial contaminant and is reported to be a risk factor associated with the development of tumors. In our previous studies, we have shown that BPA promoted the growth of SK-N-SH human neuroblastoma cells and increased their invasion and metastasis. In this study, we further investigated the effects of BPA and 17β-estradiol (E2) on the stem cell-like cells from SK-N-SH cells. Detection of stem cell markers, proliferation assay, and clonogenic analysis showed that the side-population (SP) of SK-N-SH cells had properties similar to those of stem cells. BPA or E2 exposure decreased the percentage of SP cells and the expression of stem cell-marker proteins. BPA and E2 promoted the growth of non-SP cells to a greater extent than of SP cells; in addition, they significantly increased the growth of SP cells. Thus, BPA has effects on stem cell-like cells, which induce tumor formation, and thus, BPA is an environmental factor that plays an important role in the development of neuroblastoma.
authors.affiliation: Department of Pediatric Surgery, Children's Hospital, Fudan University, Shanghai, China.
authors.forename: J C
authors.initials: JC
authors.lastname: Zheng
authors.affiliation: Department of Pediatric Surgery, Anhui Provincial Children's Hospital, Hefei, China.
authors.forename: S Q
authors.initials: SQ
authors.lastname: Qi
authors.affiliation: Department of Pediatric Surgery, Children's Hospital, Fudan University, Shanghai, China.
authors.forename: L
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Pediatric Surgery, Children's Hospital, Fudan University, Shanghai, China.
authors.forename: Y Y
authors.initials: YY
authors.lastname: Ma
authors.affiliation: Department of Pediatric Surgery, Children's Hospital, Fudan University, Shanghai, China.
authors.forename: K R
authors.initials: KR
authors.lastname: Dong
authors.affiliation: Department of Pediatric Surgery, Children's Hospital, Fudan University, Shanghai, China.
authors.forename: H T
authors.initials: HT
authors.lastname: Zhu
authors.affiliation: Department of Pediatric Surgery, Children's Hospital, Fudan University, Shanghai, China.
authors.forename: S B
authors.initials: SB
authors.lastname: Yang
authors.affiliation: Department of Pediatric Surgery, Children's Hospital, Fudan University, Shanghai, China.
authors.forename: T
authors.initials: T
authors.lastname: Xu
authors.affiliation: Department of Pediatric Surgery, Children's Hospital, Fudan University, Shanghai, China.
authors.forename: S
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Department of Pediatric Surgery, Children's Hospital, Fudan University, Shanghai, China xxianmin@126.com.
authors.forename: X M
authors.initials: XM
authors.lastname: Xiao
mesh_terms: D001559:Benzhydryl Compounds / Q000633:toxicity*; D048708:Cell Growth Processes / Q000187:drug effects; D045744:Cell Line, Tumor; D004958:Estradiol / Q000633:toxicity; D006801:Humans; D014411:Neoplastic Stem Cells / Q000187:drug effects* / Q000473:pathology; D009447:Neuroblastoma / Q000473:pathology*; D010636:Phenols / Q000633:toxicity*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004958:Estradiol; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds