title: In Silico Functional and Structural Characterization of H1N1 Influenza A Viruses Hemagglutinin, 2010-2013, Shiraz, Iran.
abstract: Hemagglutinin (HA) is a major virulence factor of influenza viruses and plays an important role in viral pathogenesis. Analysis of amino acid changes, epitopes' regions, glycosylation and phosphorylation sites have greatly contributed to the development of new generations of vaccine. The hemagglutinins of 10 selected isolates, 8 of 2010 and 2 of 2013 samples were sequenced and analyzed by several bioinformatic softwares and the results were compared with those of 3 vaccine isolates. The study detected several amino acid changes related to altered epitopes' sites, modification sites and physico-chemical properties. The results showed some conserved modification sites in HA structure. This study is the first analytical research on isolates obtained from Shiraz, Iran, and our results can be used to better understand the genetic diversity and antigenic variations in Iranian and Asian H1N1 pathogenic strains.
authors.affiliation: Influenza Research Center, Department of Bacteriology and Virology, Shiraz University of Medical Sciences, 71348-45794, Shiraz, Iran, Moattaria@sums.ac.ir.
authors.forename: Afagh
authors.initials: A
authors.lastname: Moattari
authors.forename: Behzad
authors.initials: B
authors.lastname: Dehghani
authors.forename: Nastaran
authors.initials: N
authors.lastname: Khodadad
authors.forename: Forogh
authors.initials: F
authors.lastname: Tavakoli
mesh_terms: D000940:Antigenic Variation / Q000235:genetics / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology*; D019295:Computational Biology / Q000379:methods; D003198:Computer Simulation; D000939:Epitopes / Q000276:immunology*; D006388:Hemagglutinins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology*; D007251:Influenza, Human / Q000276:immunology* / Q000821:virology; D007492:Iran; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012984:Software
chemical_list: D000956:Antigens, Viral; D000939:Epitopes; D006388:Hemagglutinins; D012333:RNA, Messenger