title: A sensitive molecularly imprinted polymer based quartz crystal microbalance nanosensor for selective determination of lovastatin in red yeast rice.
abstract: Lovastatin (LOV) is a statin, used to lower cholesterol which has been found as a hypolipidemic agent in commercial red yeast rice. In present study, a sensitive molecular imprinted quartz crystal microbalance (QCM) sensor was prepared by fabricating a self-assembling monolayer formation of allylmercaptane on QCM chip surface for selective determination of lovastatin (LOV) in red yeast rice. To prepare molecular imprinted quartz crystal microbalance (QCM) nanosensor, LOV imprinted poly(2-hydroxyethyl methacrylate-methacryloylamidoaspartic acid) [p(HEMA-MAAsp)] nanofilm was attached on the modified gold surface of QCM chip. The non-modified and improved surfaces were characterized by using contact angle, atomic force microscopy (AFM) and Fourier transform infrared (FTIR) spectroscopy. The imprinted QCM sensor was validated according to the ICH guideline (International Conference on Harmonisation). The linearity range was obtained as 0.10-1.25 nM. The detection limit of the prepared material was calculated as 0.030 nM. The developed QCM nanosensor was successfully used to examine red yeast rice. Furthermore, the stability and repeatability of the prepared QCM nanosensor were studied. The spectacular long-term stability and repeatability of the prepared LOV-imprinted QCM nanosensor make them intriguing for use in QCM sensors.
authors.affiliation: Pamukkale University, Department of Chemical Engineering, Denizli, Turkey.
authors.forename: Tanju
authors.initials: T
authors.lastname: Eren
authors.affiliation: Pamukkale University, Department of Chemical Engineering, Denizli, Turkey. Electronic address: necipatar@gmail.com.
authors.forename: Necip
authors.initials: N
authors.lastname: Atar
authors.affiliation: Sinop University, Department of Metallurgical and Materials Engineering, Sinop, Turkey.
authors.forename: Mehmet Lütfi
authors.initials: ML
authors.lastname: Yola
authors.affiliation: Department of Chemistry, Graduate University of Advanced Technology, Kerman, Iran. Electronic address: h.karimi.maleh@gmail.com.
authors.forename: Hassan
authors.initials: H
authors.lastname: Karimi-Maleh
mesh_terms: D001688:Biological Products / Q000737:chemistry*; D005504:Food Analysis / Q000379:methods; D006046:Gold / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D008148:Lovastatin / Q000032:analysis*; D018625:Microscopy, Atomic Force; D054802:Molecular Imprinting; D036103:Nanotechnology; D011108:Polymers / Q000737:chemistry; D058525:Quartz Crystal Microbalance Techniques; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
chemical_list: D001688:Biological Products; D011108:Polymers; C117343:red yeast rice; D006046:Gold; D008148:Lovastatin
keywords: Lovastatin; Molecularly imprinting; Nanosensor; QCM; Validation