title: Compressive mechanical stress may activate IKK-NF-κB through proinflammatory cytokines in MC3T3-E1 cells.
abstract: OBJECTIVE
  To determine whether IKK-NF-κB is activated either directly by compressive mechanical stress or by proinflammatory cytokines produced by MC3T3-E1 cells under compressive stress loading.
  
  
  RESULTS
  MC3T3-E1 cells subjected to cyclic uniaxial compressive stress showed increased expression of proinflammatory cytokines and activation of the IKK-NF-κB signaling pathway with nuclear translocation of p65. Following treatment with antibodies to neutralize the action of the proinflammatory cytokines, IL-1β and IL-6, the activation of IKK-NF-κB signaling was notably inhibited in MC3T3-E1 cells subjected to force loading.
  
  
  CONCLUSIONS
  IKK-NF-κB signaling in MC3T3-E1 cells may be activated by proinflammatory cytokines that are produced as a consequence of mechanical stress loading and not by direct compressive mechanical stress.
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China, 532335421@qq.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Lu
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Zheng
authors.forename: Wanlu
authors.initials: W
authors.lastname: Lu
authors.forename: Junli
authors.initials: J
authors.lastname: Yue
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.forename: Xuedong
authors.initials: X
authors.lastname: Zhou
authors.forename: Lan
authors.initials: L
authors.lastname: Zhang
authors.forename: Dingming
authors.initials: D
authors.lastname: Huang
mesh_terms: D000818:Animals; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism*; D005786:Gene Expression Regulation; D020672:I-kappa B Proteins / Q000378:metabolism*; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D015398:Signal Transduction; D013314:Stress, Mechanical
chemical_list: D016207:Cytokines; C083487:I kappa B beta protein; D020672:I-kappa B Proteins; D016328:NF-kappa B