title: TRP Channels in Insect Stretch Receptors as Insecticide Targets.
abstract: Defining the molecular targets of insecticides is crucial for assessing their selectivity and potential impact on environment and health. Two commercial insecticides are now shown to target a transient receptor potential (TRP) ion channel complex that is unique to insect stretch receptor cells. Pymetrozine and pyrifluquinazon disturbed Drosophila coordination and hearing by acting on chordotonal stretch receptor neurons. This action required the two TRPs Nanchung (Nan) and Inactive (Iav), which co-occur exclusively within these cells. Nan and Iav together sufficed to confer cellular insecticide responses in vivo and in vitro, and the two insecticides were identified as specific agonists of Nan-Iav complexes that, by promoting cellular calcium influx, silence the stretch receptor cells. This establishes TRPs as insecticide targets and defines specific agonists of insect TRPs. It also shows that TRPs can render insecticides cell-type selective and puts forward TRP targets to reduce side effects on non-target species.
authors.affiliation: BASF Corporation, Research Triangle Park, NC 27709, USA.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Nesterov
authors.affiliation: Department of Cellular Neurobiology, University of Göttingen, 37077 Göttingen, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Spalthoff
authors.affiliation: BASF Corporation, Research Triangle Park, NC 27709, USA.
authors.forename: Ramani
authors.initials: R
authors.lastname: Kandasamy
authors.affiliation: Department of Cellular Neurobiology, University of Göttingen, 37077 Göttingen, Germany.
authors.forename: Radoslav
authors.initials: R
authors.lastname: Katana
authors.affiliation: BASF Corporation, Research Triangle Park, NC 27709, USA.
authors.forename: Nancy B
authors.initials: NB
authors.lastname: Rankl
authors.affiliation: Department of Cellular Neurobiology, University of Göttingen, 37077 Göttingen, Germany.
authors.forename: Marta
authors.initials: M
authors.lastname: Andrés
authors.affiliation: Department of Cellular Neurobiology, University of Göttingen, 37077 Göttingen, Germany.
authors.forename: Philipp
authors.initials: P
authors.lastname: Jähde
authors.affiliation: BASF Corporation, Research Triangle Park, NC 27709, USA.
authors.forename: John A
authors.initials: JA
authors.lastname: Dorsch
authors.affiliation: BASF Corporation, Research Triangle Park, NC 27709, USA.
authors.forename: Lynn F
authors.initials: LF
authors.lastname: Stam
authors.affiliation: BASF Corporation, Research Triangle Park, NC 27709, USA.
authors.forename: Franz-Josef
authors.initials: FJ
authors.lastname: Braun
authors.affiliation: Department of Cellular Neurobiology, University of Göttingen, 37077 Göttingen, Germany.
authors.forename: Ben
authors.initials: B
authors.lastname: Warren
authors.affiliation: BASF Corporation, Research Triangle Park, NC 27709, USA. Electronic address: vincent.salgado@basf.com.
authors.forename: Vincent L
authors.initials: VL
authors.lastname: Salgado
authors.affiliation: Department of Cellular Neurobiology, University of Göttingen, 37077 Göttingen, Germany. Electronic address: mgoepfe@gwdg.de.
authors.forename: Martin C
authors.initials: MC
authors.lastname: Göpfert
mesh_terms: D000161:Acoustic Stimulation; D000200:Action Potentials / Q000187:drug effects / Q000235:genetics; D000818:Animals; D030801:Animals, Genetically Modified; D057506:Arthropod Antennae / Q000166:cytology; D016466:CHO Cells; D003412:Cricetulus; D004330:Drosophila; D029721:Drosophila Proteins / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D018466:Gravity Sensing / Q000187:drug effects / Q000235:genetics; D007473:Ion Channels / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D007814:Larva; D008465:Mechanoreceptors / Q000187:drug effects* / Q000502:physiology; D009068:Movement / Q000187:drug effects; D009474:Neurons / Q000187:drug effects; D010575:Pesticides / Q000494:pharmacology*; D052999:Quinazolinones / Q000494:pharmacology*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D050051:Transient Receptor Potential Channels / Q000187:drug effects* / Q000235:genetics; D014227:Triazines / Q000494:pharmacology*; D005466:Fluorocarbons
chemical_list: D029721:Drosophila Proteins; D007473:Ion Channels; D010575:Pesticides; D052999:Quinazolinones; D014157:Transcription Factors; D050051:Transient Receptor Potential Channels; D014227:Triazines; C557617:GAL4 protein, Drosophila; C494780:IAV protein, Drosophila; C476010:Nan protein, Drosophila; C480311:pymetrozine; C581681:1,1,3,4-tetrahydro-6-(1,2,2,2-tetrafluoro-1-(trifluoromethyl)ethyl)quinazolin-2,4-dione; D005466:Fluorocarbons