title: Multibiomarker in fish to evaluate a river used to water public supply.
abstract: We aimed to evaluate the ecological integrity of a large river, which receives agricultural and urban effluents and is used to water public supply. The fish species Astyanax bifasciatus was used as bioindicator during winter and spring 2012, and summer 2013 at the Middle Iguaçu River basin in Paraná state, Brazil. Water chemical and physical measures and ecotoxicological tests were carried out as well biochemical and genetic biomarkers in sampled fish in each period. The studied area was divided in three sample points: SP1, located where the water is collected to public supply; SP2, located in an urbanized area, and SP3, located at an urbanized area with the discharge of the sewage treatment. Although water chemical and physical analyzes were range of the Brazilian law to hydric bodies, anticholinesterasic effects were found in winter, oxidative stress in summer and spring. The higher genotoxic effect was in winter to all sample points. The temporal variation in biomarkers and the detection of caffeine in the water call attention to the water quality in this river mainly to be used to public supply.
authors.affiliation: Ecology and Conservation Post-graduate Program, Federal University of Paraná, P.O. Box 19031, Curitiba-Paraná 81531-990, Brazil. Electronic address: bueno_acd@yahoo.com.br.
authors.forename: A C D
authors.initials: AC
authors.lastname: Bueno-Krawczyk
authors.affiliation: Department of Pharmacology, Federal University of Paraná, P.O. Box 19031, Curitiba-Paraná 81531-990, Brazil.
authors.forename: I C
authors.initials: IC
authors.lastname: Guiloski
authors.affiliation: Department of Genetics, Federal University of Paraná, P.O. Box 19031, Curitiba-Paraná 81531-990, Brazil.
authors.forename: L D S
authors.initials: LD
authors.lastname: Piancini
authors.affiliation: Environmental Science and Technology Post-graduate Program, Paraná Federal Technological University, Curitiba, PR, Brazil.
authors.forename: J C
authors.initials: JC
authors.lastname: Azevedo
authors.affiliation: Environmental Science and Technology Post-graduate Program, Paraná Federal Technological University, Curitiba, PR, Brazil.
authors.forename: W A
authors.initials: WA
authors.lastname: Ramsdorf
authors.affiliation: Environmental Science and Technology Post-graduate Program, Paraná Federal Technological University, Curitiba, PR, Brazil.
authors.forename: A H
authors.initials: AH
authors.lastname: Ide
authors.affiliation: Department of Biological Sciences and Health, West Paraná State University, Cascavel, PR, Brazil.
authors.forename: A T B
authors.initials: AT
authors.lastname: Guimarães
authors.affiliation: Department of Genetics, Federal University of Paraná, P.O. Box 19031, Curitiba-Paraná 81531-990, Brazil.
authors.forename: M M
authors.initials: MM
authors.lastname: Cestari
authors.affiliation: Department of Pharmacology, Federal University of Paraná, P.O. Box 19031, Curitiba-Paraná 81531-990, Brazil.
authors.forename: H C
authors.initials: HC
authors.lastname: Silva de Assis
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001938:Brazil; D004784:Environmental Monitoring / Q000379:methods*; D005399:Fishes / Q000378:metabolism*; D045483:Rivers; D012621:Seasons; D014867:Water; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*; D060753:Water Quality; D014881:Water Supply
chemical_list: D015415:Biomarkers; D014874:Water Pollutants, Chemical; D014867:Water
keywords: Acetylcholinesterase; Biomarker; Biomonitoring; Ecotoxicology; Oxidative stress