title: MiR-144 Inhibits Uveal Melanoma Cell Proliferation and Invasion by Regulating c-Met Expression.
abstract: MicroRNAs (miRNAs) are a group endogenous small non-coding RNAs that inhibit protein translation through binding to specific target mRNAs. Recent studies have demonstrated that miRNAs are implicated in the development of cancer. However, the role of miR-144 in uveal melanoma metastasis remains largely unknown. MiR-144 was downregulated in both uveal melanoma cells and tissues. Transfection of miR-144 mimic into uveal melanoma cells led to a decrease in cell growth and invasion. After identification of two putative miR-144 binding sites within the 3' UTR of the human c-Met mRNA, miR-144 was proved to inhibit the luciferase activity inMUM-2B cells with a luciferase reporter construct containing the binding sites. In addition, the expression of c-Met protein was inhibited by miR-144. Furthermore, c-Met-mediated cell proliferation and invasion were inhibited by restoration of miR-144 in uveal melanoma cells. In conclusion, miR-144 acts as a tumor suppressor in uveal melanoma, through inhibiting cell proliferation and migration. miR-144 might serve as a potential therapeutic target in uveal melanoma patients.
authors.affiliation: Department of Ophthalmology, The Fourth Hospital of Harbin Medical University, Harbin, 150001, Heilongjiang, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Sun
authors.affiliation: Department of Ophthalmology, Shandong Provincial Qianfoshan Hospital, Jinan 250000, Shandong, China.
authors.forename: Guangqing
authors.initials: G
authors.lastname: Bian
authors.affiliation: Department of Ophthalmology, The Fourth Hospital of Harbin Medical University, Harbin, 150001, Heilongjiang, China.
authors.forename: ZhaoJun
authors.initials: Z
authors.lastname: Meng
authors.affiliation: Department of Ophthalmology, Shandong Provincial Qianfoshan Hospital, Jinan 250000, Shandong, China.
authors.forename: Guangfu
authors.initials: G
authors.lastname: Dang
authors.affiliation: Department of Ophthalmology, The Fourth Hospital of Harbin Medical University, Harbin, 150001, Heilongjiang, China.
authors.forename: DeJing
authors.initials: D
authors.lastname: Shi
authors.affiliation: Department of Ophthalmology, The Fourth Hospital of Harbin Medical University, Harbin, 150001, Heilongjiang, China.
authors.forename: Shuyong
authors.initials: S
authors.lastname: Mi
mesh_terms: D020413:3' Untranslated Regions; D001483:Base Sequence; D001665:Binding Sites; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008545:Melanoma / Q000235:genetics* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics*; D019859:Proto-Oncogene Proteins c-met / Q000235:genetics*; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics*; D014604:Uveal Neoplasms / Q000235:genetics* / Q000473:pathology; D000098943:Uveal Melanoma
chemical_list: D020413:3' Untranslated Regions; C554293:MIRN144 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D019859:Proto-Oncogene Proteins c-met