title: Hydrothermal-acid treatment for effectual extraction of eicosapentaenoic acid (EPA)-abundant lipids from Nannochloropsis salina.
abstract: Hydrothermal acid treatment, was adopted to extract eicosapentaenoic acid (EPA) from wet biomass of Nannochloropsis salina. It was found that sulfuric acid-based treatment increased EPA yield from 11.8 to 58.1 mg/g cell in a way that was nearly proportional to its concentration. Nitric acid exhibited the same pattern at low concentrations, but unlike sulfuric acid its effectiveness unexpectedly dropped from 0.5% to 2.0%. The optimal and minimal conditions for hydrothermal acid pretreatment were determined using a statistical approach; its maximum EPA yield (predicted: 43.69 mg/g cell; experimental: 43.93 mg/g cell) was established at a condition of 1.27% of sulfuric acid, 113.34 °C of temperature, and 36.71 min of reaction time. Our work demonstrated that the acid-catalyzed cell disruption, accompanied by heat, can be one potentially promising option for ω-3 fatty acids extraction.
authors.affiliation: Department of Civil and Environmental Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 305-338, Republic of Korea.
authors.forename: Ilgyu
authors.initials: I
authors.lastname: Lee
authors.affiliation: Department of Civil and Environmental Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 305-338, Republic of Korea. Electronic address: jihan@kaist.ac.kr.
authors.forename: Jong-In
authors.initials: JI
authors.lastname: Han
mesh_terms: D018533:Biomass; D015118:Eicosapentaenoic Acid / Q000737:chemistry*; D015525:Fatty Acids, Omega-3 / Q000737:chemistry; D008055:Lipids / Q000737:chemistry*; D058086:Microalgae / Q000737:chemistry*; D017942:Nitric Acid / Q000737:chemistry; D058009:Stramenopiles / Q000737:chemistry*; D013464:Sulfuric Acids / Q000737:chemistry; D013696:Temperature
chemical_list: D015525:Fatty Acids, Omega-3; D008055:Lipids; D013464:Sulfuric Acids; D017942:Nitric Acid; D015118:Eicosapentaenoic Acid; C033158:sulfuric acid
keywords: Cell disruption; Eicosapentaenoic acid (EPA); Lipid extraction; Microalgae; ω-3 Polyunsaturated fatty acid (PUFA)