title: Differential gene expression in human abdominal aortic aneurysm and aortic occlusive disease.
abstract: Abdominal aortic aneurysm (AAA) and aortic occlusive disease (AOD) represent common causes of morbidity and mortality in elderly populations which were previously believed to have common aetiologies. The aim of this study was to assess the gene expression in human AAA and AOD. We performed microarrays using aortic specimen obtained from 20 patients with small AAAs (≤ 55mm), 29 patients with large AAAs (> 55mm), 9 AOD patients, and 10 control aortic specimens obtained from organ donors. Some differentially expressed genes were validated by quantitative-PCR (qRT-PCR)/immunohistochemistry. We identified 840 and 1,014 differentially expressed genes in small and large AAAs, respectively. Immune-related pathways including cytokine-cytokine receptor interaction and T-cell-receptor signalling were upregulated in both small and large AAAs. Examples of validated genes included CTLA4 (2.01-fold upregulated in small AAA, P = 0.002), NKTR (2.37-and 2.66-fold upregulated in small and large AAA with P = 0.041 and P = 0.015, respectively), and CD8A (2.57-fold upregulated in large AAA, P = 0.004). 1,765 differentially expressed genes were identified in AOD. Pathways upregulated in AOD included metabolic and oxidative phosphorylation categories. The UCP2 gene was downregulated in AOD (3.73-fold downregulated, validated P = 0.017). In conclusion, the AAA and AOD transcriptomes were very different suggesting that AAA and AOD have distinct pathogenic mechanisms.
authors.affiliation: The Queensland Research Centre for Peripheral Vascular Disease, College of Medicine and Dentistry, James Cook University, Townsville, Queensland, Australia.
authors.forename: Erik
authors.initials: E
authors.lastname: Biros
authors.affiliation: Department of Vascular and Endovascular Surgery, Ludwig-Maximillian University, Munich, Germany.
authors.forename: Gabor
authors.initials: G
authors.lastname: Gäbel
authors.affiliation: The Queensland Research Centre for Peripheral Vascular Disease, College of Medicine and Dentistry, James Cook University, Townsville, Queensland, Australia.
authors.forename: Corey S
authors.initials: CS
authors.lastname: Moran
authors.affiliation: Department of Vascular Surgery, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Schreurs
authors.affiliation: Department of Vascular Surgery, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Jan H N
authors.initials: JH
authors.lastname: Lindeman
authors.affiliation: Royal Brisbane Clinical School, The University of Queensland, Queensland, Australia.
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Walker
authors.affiliation: The Cardiovascular Research Group, Department of Medicine, The University of Queensland, Queensland, Australia.
authors.forename: Maria
authors.initials: M
authors.lastname: Nataatmadja
authors.affiliation: The Cardiovascular Research Group, Department of Medicine, The University of Queensland, Queensland, Australia.
authors.forename: Malcolm
authors.initials: M
authors.lastname: West
authors.affiliation: Institute of Laboratory Medicine, Ludwig Maximilians University Munich, Munich, Germany.
authors.forename: Lesca M
authors.initials: LM
authors.lastname: Holdt
authors.affiliation: Department of General, Visceral, Vascular and Thoracic Surgery, Charité Universitätsmedizin Berlin, Charité Campus Mitte, Berlin, Germany.
authors.forename: Irene
authors.initials: I
authors.lastname: Hinterseher
authors.affiliation: Department of Vascular, Thoracic and Visceral Surgery, TU-Dresden, Dresden, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Pilarsky
authors.affiliation: The Queensland Research Centre for Peripheral Vascular Disease, College of Medicine and Dentistry, James Cook University, Townsville, Queensland, Australia.|Department of Vascular and Endovascular Surgery, The Townsville Hospital, Townsville, Queensland, Australia.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Golledge
mesh_terms: D000368:Aged; D001012:Aorta, Abdominal / Q000473:pathology; D017544:Aortic Aneurysm, Abdominal / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D001157:Arterial Occlusive Diseases / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D016022:Case-Control Studies; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction
keywords: Pathology Section; abdominal; aortic aneurysm; arterial occlusive diseases; tissue array analysis