title: Effect of superabsorbent dressings in a 3D acellular tissue model of Pseudomonas aeruginosa biofilm.
abstract: OBJECTIVE
  To compare a superabsorbent polymer dressing (DryMax Extra; DME), an antibacterial absorbent polymer dressing (Sorbact absorption dressing; SB) and an antibacterial superabsorbent polymer dressing (Sorbion Sachet S; SSS) activity against Pseudomonas aeruginosa biofilms.
  
  
  METHODS
  A 3D acellular synthetic soft tissue (ASST) allowing biofilm formation, was prepared and inoculated with Pseudomonas aeruginosa, before the application of dressings. The dressings DME, with and without a silver net, and two benchmark dressings SB and SSS were tested. After 24 hours' incubation, qualitative assessment by visual screening of the soft tissue and bacterial burden assessment in the dressings and acellular soft tissue model were performed.
  
  
  RESULTS
  DME combined with a silver net gave a distinct and wide colourless zone of inhibition while partial zones of inhibition were seen for DME, SSS and SB. Compared with the tissues exposed to the other dressings, those exposed to SB and the bacterial control appeared green and opaque. In descending order, the most visual growth was seen in bacterial control, followed by SB, SSS, and DME. The bacterial load was equivalent for all dressings without an antimicrobial substance in both ASST (around log 10) and dressing (around log 11). The bacterial load for DME combined with a silver net, in comparison to DME alone was significantly reduced, with log 3.6 in dressings and log 4.2 in ASST.
  
  
  CONCLUSIONS
  The removal of bacteria by DME was equivalent to SB and SSS. Furthermore, DME limited the production of the green colour, indicative of Pyocyanin. If extrapolated to a wound, the ability of DME to absorb Pseudomonas aeruginosa and limit Pyocyanin levels in the wound might lead to reduced virulence.
authors.affiliation: Scientific Project Manager.
authors.forename: E
authors.initials: E
authors.lastname: Larkö
authors.forename: A
authors.initials: A
authors.lastname: Persson
authors.forename: K
authors.initials: K
authors.lastname: Blom
mesh_terms: D062088:Acellular Dermis / Q000382:microbiology*; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D058491:Bacterial Load; D001458:Bandages / Q000382:microbiology*; D018441:Biofilms / Q000187:drug effects*; D006801:Humans; D011108:Polymers; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000472:pathogenicity; D011710:Pyocyanine; D018030:Silver Compounds / Q000494:pharmacology; D014774:Virulence
chemical_list: D000900:Anti-Bacterial Agents; D011108:Polymers; D018030:Silver Compounds; D011710:Pyocyanine
keywords: 3D tissue; Pseudomonas aeruginosa; antibacterial; biofilm; pyocyanin; superabsorbent dressings