title: A Single-Vector, Single-Injection Trivalent Filovirus Vaccine: Proof of Concept Study in Outbred Guinea Pigs.
abstract: The filoviruses, Marburg marburgvirus (MARV), Zaire ebolavirus (ZEBOV), and Sudan ebolavirus (SEBOV), cause severe and often fatal hemorrhagic fever in humans and nonhuman primates (NHPs). Monovalent recombinant vesicular stomatitis virus (rVSV)-based vaccine vectors, which encode a filovirus glycoprotein (GP) in place of the VSV glycoprotein, have shown 100% efficacy against homologous filovirus challenge in rodent and NHP studies. Here, we examined the utility of a single-vector, single-injection trivalent rVSV vector expressing MARV, ZEBOV, and SEBOV GPs to protect against MARV-, ZEBOV-, and SEBOV-induced disease in outbred Hartley guinea pigs where we observed protection from effects of all 3 filoviruses.
authors.affiliation: Galveston National Laboratory Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas.
authors.forename: Chad E
authors.initials: CE
authors.lastname: Mire
authors.affiliation: Galveston National Laboratory Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas.
authors.forename: Joan B
authors.initials: JB
authors.lastname: Geisbert
authors.affiliation: Galveston National Laboratory Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas.
authors.forename: Krista M
authors.initials: KM
authors.lastname: Versteeg
authors.affiliation: Department of Microbiology, Boston University School of Medicine National Emerging Infectious Diseases Laboratory, Boston, Massachusetts.
authors.forename: Natalia
authors.initials: N
authors.lastname: Mamaeva
authors.affiliation: Galveston National Laboratory Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas.
authors.forename: Krystle N
authors.initials: KN
authors.lastname: Agans
authors.affiliation: Galveston National Laboratory Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas.
authors.forename: Thomas W
authors.initials: TW
authors.lastname: Geisbert
authors.affiliation: Department of Microbiology, Boston University School of Medicine National Emerging Infectious Diseases Laboratory, Boston, Massachusetts.
authors.forename: John H
authors.initials: JH
authors.lastname: Connor
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D005260:Female; D016563:Filoviridae / Q000276:immunology*; D018702:Filoviridae Infections / Q000276:immunology* / Q000821:virology; D005822:Genetic Vectors / Q000276:immunology*; D006023:Glycoproteins / Q000276:immunology; D006168:Guinea Pigs; D018116:Vesiculovirus / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D006023:Glycoproteins; D014765:Viral Vaccines
keywords: Ebola virus; Marburg virus; cross-protection; filovirus; guinea pig; trivalent; vaccine; vesicular stomatitis virus