title: Differential adaptive responses to 1- or 2-day fasting in various mouse tissues revealed by quantitative PCR analysis.
abstract: Dietary or caloric restriction confers various clinical benefits. Short-term fasting of mice is a common experimental procedure that may involve systemic metabolic remodeling, which may significantly affect experimental outputs. This study evaluated adaptive cellular responses after 1- or 2-day fasting in 13 mouse tissues by quantitative PCR using 15 marker primer sets for the activation of ubiquitin-proteasome (Atrogin-1 and MuRF1), autophagy-lysosome (LC3b, p62 and Lamp2), amino acid response (Asns, Trib3, Herpud1, xCT, and Chop), Nrf2-mediated antioxidant (HO-1 and Gsta1), and amino acid transport (Slc38a2, Slc7a5, and Slc7a1) systems. Differential activation profiles obtained in seven highly (thymus, liver, spleen, and small intestine) or mildly (stomach, kidney, and colon) atrophied tissues as well as in six non-atrophied tissues (brain, eye, lung, heart, skeletal muscle, and testis) suggested tissue-specific active metabolic remodeling.
authors.affiliation: Department of Biochemistry, Keio University Graduate School of Pharmaceutical Sciences, Japan.
authors.forename: Junya
authors.initials: J
authors.lastname: Yamamoto
authors.affiliation: Department of Biochemistry, Keio University Graduate School of Pharmaceutical Sciences, Japan.
authors.forename: Shotaro
authors.initials: S
authors.lastname: Kamata
authors.affiliation: Department of Biochemistry, Keio University Graduate School of Pharmaceutical Sciences, Japan.
authors.forename: Asumi
authors.initials: A
authors.lastname: Miura
authors.affiliation: Department of Biochemistry, Keio University Graduate School of Pharmaceutical Sciences, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Nagata
authors.affiliation: Department of Biochemistry, Keio University Graduate School of Pharmaceutical Sciences, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Kainuma
authors.affiliation: Department of Biochemistry, Keio University Graduate School of Pharmaceutical Sciences, Japan.
authors.forename: Isao
authors.initials: I
authors.lastname: Ishii
keywords: AL, ad libitum-fed; AST, aspartate aminotransferase; Amino acid response; Amino acid transport; Autophagy; BUN, blood urea nitrogen; CPK, creatine phosphokinase; CT, computed tomography; ER, endoplasmic reticulum; LDH, lactate dehydrogenase; Nrf2; Proteasome; TG, triglyceride; Ubiquitin; qPCR, quantitative PCR