title: Role of the CKIP1 gene in proliferation and apoptosis of the human lung cancer cell line H1299.
abstract: Casein kinase 2 interacting protein 1 (CKIP1) is a specific interacting protein of the casein kinase 2 (CK2) α subunit, and, by binding CK2 and other proteins, functions as an adaptor to regulate a series of cellular functions. Previous studies suggested that CKIP1 might play an important role in regulating oncogenic activities. However, few studies examining the function of CKIP1 in cancer cells have been performed. The present study aimed to investigate the role of CKIP1 in lung cancer. CKIP1 mRNA expression was detected in 5 human lung cancer cell lines (H-125, H1299, LTEP-A-2, SPC-A-1, and NCL-H446) by semi-quantitative RT-PCR, and in 10 noncancerous lung tissues and 30 non-small lung cancer tissues by real-time quantitative PCR. A lentivirus-mediated small interfering RNA (siRNA) was used to knock down CKIP1 expression in the H1299 cell line. To elucidate the impact of CKIP1 downregulation on H1299 cells, cell proliferation, DNA synthesis, and cell cycle distribution and apoptosis were measured by high content screening assay, BrdU incorporation, and flow cytometric analyses, respectively. CKIP1 mRNA was highly expressed both in H1299 cells and lung cancer tissues. We found that downregulation of CKIP1 resulted in suppression of proliferation and colony-forming ability of H1299 cells, and led to S phase cell cycle arrest and G2 phase promotion, as well as a significant enhancement of H1299 cell apoptosis. Our study indicated that high expression levels of CKIP1 were associated with the development of lung cancer, and that CKIP1 knockdown may block tumor cell growth mainly by promoting cell apoptosis.
authors.affiliation: Surgical Department, Dongguan People's Hospital, Dongguan City, China.
authors.forename: G M
authors.initials: GM
authors.lastname: Chen
authors.affiliation: Department of Medical Oncology, Dongguan People's Hospital, Dongguan City, China.
authors.forename: R F
authors.initials: RF
authors.lastname: Ding
authors.affiliation: Department of Medical Oncology, Dongguan People's Hospital, Dongguan City, China.
authors.forename: Y D
authors.initials: YD
authors.lastname: Tan
authors.affiliation: Department of Medical Oncology, Dongguan People's Hospital, Dongguan City, China.
authors.forename: X B
authors.initials: XB
authors.lastname: Pan
authors.affiliation: Department of Medical Oncology, Dongguan People's Hospital, Dongguan City, China.
authors.forename: G M
authors.initials: GM
authors.lastname: Jiang
authors.affiliation: Pathology Department, Dongguan People's Hospital, Dongguan City, China.
authors.forename: J F
authors.initials: JF
authors.lastname: He
authors.affiliation: Department of Medical Oncology, Dongguan People's Hospital, Dongguan City, China.
authors.forename: S H
authors.initials: SH
authors.lastname: Lin
authors.affiliation: Department of Medical Oncology, Dongguan People's Hospital, Dongguan City, China.
authors.forename: C
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Medical Oncology, Dongguan People's Hospital, Dongguan City, China dgryjy@hotmail.com.
authors.forename: Y
authors.initials: Y
authors.lastname: Jia
mesh_terms: D017209:Apoptosis; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D002453:Cell Cycle; D059447:Cell Cycle Checkpoints; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D008175:Lung Neoplasms / Q000235:genetics*; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; C408877:PLEKHO1 protein, human; D012333:RNA, Messenger; D034741:RNA, Small Interfering