title: Ivermectin binds to Haemonchus contortus tubulins and promotes stability of microtubules.
abstract: Haemonchus contortus is a nematode of livestock that can cause severe disease and mortality. Ivermectin, an anti-parasitic drug that targets glutamate-gated chloride channels, is widely used in humans, livestock, companion animals and agriculture. Although an association between genetic changes to β-tubulin and exposure to ivermectin has been previously reported, direct binding between ivermectin and tubulin has not been demonstrated to date. Tubulin/microtubules are key targets for many anti-mitotic drugs used in anti-parasite and cancer therapies. We now report that ivermectin exposure increased the rate and extent of polymerisation of H. contortus recombinant α- and β-tubulin, and protected the parasitic α- and β-tubulins from limited trypsin proteolysis. Direct binding between ivermectin and the tubulin monomers exhibited low micromolar affinities, as determined using surface plasmon resonance. Subsequent equilibrium dialysis indicated that ivermectin and Taxol compete for binding to tubulin, supporting our molecular modelling that predicts ivermectin interacts with the Taxol binding pocket of both parasitic and mammalian tubulins. Collectively, our data indicate that ivermectin can bind to and stabilise microtubules (i.e., alter the tubulin polymerisation equilibrium) and this can then lead to mitotic arrest. This work extends the range of known pharmacological effects of ivermectin, and reveals its potential as an anti-mitotic agent.
authors.affiliation: Institute of Parasitology, McGill University-Macdonald Campus, Sainte-Anne-de-Bellevue, Quebec, Canada.
authors.forename: Shoaib
authors.initials: S
authors.lastname: Ashraf
authors.affiliation: Institute of Parasitology, McGill University-Macdonald Campus, Sainte-Anne-de-Bellevue, Quebec, Canada.
authors.forename: Robin N
authors.initials: RN
authors.lastname: Beech
authors.affiliation: SPR-MS Facility, McGill University, Montreal, Quebec, Canada.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Hancock
authors.affiliation: Institute of Parasitology, McGill University-Macdonald Campus, Sainte-Anne-de-Bellevue, Quebec, Canada. Electronic address: roger.prichard@mcgill.ca.
authors.forename: Roger K
authors.initials: RK
authors.lastname: Prichard
mesh_terms: D000818:Animals; D000977:Antiparasitic Agents / Q000737:chemistry / Q000494:pharmacology*; D001665:Binding Sites; D003001:Cloning, Molecular; D006189:Haemonchus / Q000187:drug effects* / Q000378:metabolism; D007559:Ivermectin / Q000737:chemistry / Q000494:pharmacology*; D008870:Microtubules / Q000187:drug effects*; D008958:Models, Molecular; D017239:Paclitaxel / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D014404:Tubulin / Q000378:metabolism*
chemical_list: D000977:Antiparasitic Agents; D014404:Tubulin; D007559:Ivermectin; D017239:Paclitaxel
keywords: Anti-mitotic; Haemonchus contortus; Ivermectin; Microtubule; Nematode; Tubulin