title: Selective modulation of the functions of a conserved DNA motor by a histone fold complex.
abstract: Budding yeast Mph1 helicase and its orthologs drive multiple DNA transactions. Elucidating the mechanisms that regulate these motor proteins is central to understanding genome maintenance processes. Here, we show that the conserved histone fold MHF complex promotes Mph1-mediated repair of damaged replication forks but does not influence the outcome of DNA double-strand break repair. Mechanistically, scMHF relieves the inhibition imposed by the structural maintenance of chromosome protein Smc5 on Mph1 activities relevant to replication-associated repair through binding to Mph1 but not DNA. Thus, scMHF is a function-specific enhancer of Mph1 that enables flexible response to different genome repair situations.
authors.affiliation: Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA;
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Xue
authors.affiliation: Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA; Programs in Biochemistry, Cell, and Molecular Biology, Weill Graduate School of Medical Sciences of Cornell University, New York, New York 10065, USA;
authors.forename: Koyi
authors.initials: K
authors.lastname: Choi
authors.affiliation: Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA; Programs in Biochemistry, Cell, and Molecular Biology, Weill Graduate School of Medical Sciences of Cornell University, New York, New York 10065, USA;
authors.forename: Jacob N
authors.initials: JN
authors.lastname: Bonner
authors.affiliation: IFOM, Fondazione Istituto FIRC di Oncologia Molecolare, Milan 20139, Italy;
authors.forename: Barnabas
authors.initials: B
authors.lastname: Szakal
authors.affiliation: Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA; Programs in Biochemistry, Cell, and Molecular Biology, Weill Graduate School of Medical Sciences of Cornell University, New York, New York 10065, USA;
authors.forename: Yu-Hung
authors.initials: YH
authors.lastname: Chen
authors.affiliation: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA.
authors.forename: Alma
authors.initials: A
authors.lastname: Papusha
authors.affiliation: Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA;
authors.forename: Dorina
authors.initials: D
authors.lastname: Saro
authors.affiliation: Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA;
authors.forename: Hengyao
authors.initials: H
authors.lastname: Niu
authors.affiliation: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Ira
authors.affiliation: IFOM, Fondazione Istituto FIRC di Oncologia Molecolare, Milan 20139, Italy;
authors.forename: Dana
authors.initials: D
authors.lastname: Branzei
authors.affiliation: Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA;
authors.forename: Patrick
authors.initials: P
authors.lastname: Sung
authors.affiliation: Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA; Programs in Biochemistry, Cell, and Molecular Biology, Weill Graduate School of Medical Sciences of Cornell University, New York, New York 10065, USA; zhaox1@mskcc.org.
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Zhao
mesh_terms: D053487:DEAD-box RNA Helicases / Q000378:metabolism; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000378:metabolism*; D016681:Genome, Fungal / Q000235:genetics; D006657:Histones / Q000378:metabolism*; D009154:Mutation; D011485:Protein Binding; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D006657:Histones; D029701:Saccharomyces cerevisiae Proteins; D004247:DNA; C491240:MPH1 protein, S cerevisiae; D053487:DEAD-box RNA Helicases
keywords: DNA motor proteins; genome replication; histone fold proteins; homologous recombination; replication fork repair; structural maintenance of chromosome proteins