title: A novel unsaturated fatty acid hydratase toward C16 to C22 fatty acids from Lactobacillus acidophilus.
abstract: Hydroxy FAs, one of the gut microbial metabolites of PUFAs, have attracted much attention because of their various bioactivities. The purpose of this study was to identify lactic acid bacteria with the ability to convert linoleic acid (LA) to hydroxy FAs. A screening process revealed that a gut bacterium, Lactobacillus acidophilus NTV001, converts LA mainly into 13-hydroxy-cis-9-octadecenoic acid and resulted in the identification of the hydratase responsible, fatty acid hydratase 1 (FA-HY1). Recombinant FA-HY1 was purified, and its enzymatic characteristics were investigated. FA-HY1 could convert not only C18 PUFAs but also C20 and C22 PUFAs. C18 PUFAs with a cis carbon-carbon double bond at the Δ12 position were converted into the corresponding 13-hydroxy FAs. Arachidonic acid and DHA were converted into the corresponding 15-hydroxy FA and 14-hydroxy FA, respectively. To the best of our knowledge, this is the first report of a bacterial FA hydratase that can convert C20 and C22 PUFAs into the corresponding hydroxy FAs. These novel hydroxy FAs produced by using FA-HY1 should contribute to elucidating the bioactivities of hydroxy FAs.
authors.affiliation: Division of Applied Life Sciences Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Hirata
authors.affiliation: Division of Applied Life Sciences Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
authors.forename: Shigenobu
authors.initials: S
authors.lastname: Kishino
authors.affiliation: Laboratory of Industrial Microbiology, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
authors.forename: Si-Bum
authors.initials: SB
authors.lastname: Park
authors.affiliation: Division of Applied Life Sciences Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
authors.forename: Michiki
authors.initials: M
authors.lastname: Takeuchi
authors.affiliation: Division of Applied Life Sciences Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
authors.forename: Nahoko
authors.initials: N
authors.lastname: Kitamura
authors.affiliation: Division of Applied Life Sciences Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Ogawa
mesh_terms: D055162:Biocatalysis; D003067:Coenzymes / Q000378:metabolism; D006867:Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D007779:Lactobacillus acidophilus / Q000201:enzymology* / Q000378:metabolism; D019787:Linoleic Acid / Q000737:chemistry* / Q000378:metabolism*; D013237:Stereoisomerism; D013379:Substrate Specificity; D014867:Water / Q000378:metabolism
chemical_list: D003067:Coenzymes; D014867:Water; D019787:Linoleic Acid; D006867:Hydrolases
keywords: arachidonic acid; dehydration; docosahexaenoic acid; enzymology; fatty acid/metabolism; hydration; hydroxy fatty acid; lactic acid bacteria; lipids; lipids/chemistry