title: Molecular cloning and characterization of annexin genes in peanut (Arachis hypogaea L.).
abstract: Annexin, Ca(2+) or phospholipid binding proteins, with many family members are distributed throughout all tissues during plant growth and development. Annexins participate in a number of physiological processes, such as exocytosis, cell elongation, nodule formation in legumes, maturation and stress response. Six different full-length cDNAs and two partial-length cDNAs of peanut, (AnnAh1, AnnAh2, AnnAh3, AnnAh5, AnnAh6, AnnAh7, AnnAh4 and AnnAh8) encoding annexin proteins, were isolated and characterized using a RT-PCR/RACE-PCR based strategy. The predicted molecular masses of these annexins were 36.0kDa with acidic pIs of 5.97-8.81. ANNAh1, ANNAh2, ANNAh3, ANNAh5, ANNAh6 and ANNAh7 shared sequence similarity from 35.76 to 66.35% at amino acid level. Phylogenetic analysis revealed their evolutionary relationships with corresponding orthologous sequences in soybean and deduced proteins in various plant species. Real-time quantitative assays indicated that these genes were differentially expressed in various organs. Transcript level analysis for six annexin genes under stress conditions showed that these genes were regulated by drought, salinity, heavy metal stress, low temperature and hormone. Additionally, the prediction of cis-regulatory element suggested that different cis-responsive elements including stress- and hormone-responsive-related elements could respond to various stress conditions. These results indicated that members of AnnAhs family may play important roles in the adaptation of peanut to various environmental stresses.
authors.affiliation: North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Laboratory of Crop Germplasm Resources of Hebei, Agricultural University of Hebei, Baoding 071001, People's Republic of China.
authors.forename: MeiJing
authors.initials: M
authors.lastname: He
authors.affiliation: North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Laboratory of Crop Germplasm Resources of Hebei, Agricultural University of Hebei, Baoding 071001, People's Republic of China.
authors.forename: XinLei
authors.initials: X
authors.lastname: Yang
authors.affiliation: North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Laboratory of Crop Germplasm Resources of Hebei, Agricultural University of Hebei, Baoding 071001, People's Republic of China.
authors.forename: ShunLi
authors.initials: S
authors.lastname: Cui
authors.affiliation: North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Laboratory of Crop Germplasm Resources of Hebei, Agricultural University of Hebei, Baoding 071001, People's Republic of China.
authors.forename: GuoJun
authors.initials: G
authors.lastname: Mu
authors.affiliation: North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Laboratory of Crop Germplasm Resources of Hebei, Agricultural University of Hebei, Baoding 071001, People's Republic of China.
authors.forename: MingYu
authors.initials: M
authors.lastname: Hou
authors.affiliation: North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Laboratory of Crop Germplasm Resources of Hebei, Agricultural University of Hebei, Baoding 071001, People's Republic of China.
authors.forename: HuanYing
authors.initials: H
authors.lastname: Chen
authors.affiliation: North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Laboratory of Crop Germplasm Resources of Hebei, Agricultural University of Hebei, Baoding 071001, People's Republic of China. Electronic address: lifengliucau@126.com.
authors.forename: LiFeng
authors.initials: L
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D017302:Annexins / Q000235:genetics*; D010367:Arachis / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013312:Stress, Physiological
chemical_list: D017302:Annexins; D010940:Plant Proteins
keywords: Annexin genes; Cloning; Expression analysis; Peanut