title: Two new species of the genus Micromonospora: Micromonospora palomenae sp. nov. and Micromonospora harpali sp. nov. isolated from the insects.
abstract: Two novel actinobacteria, strains NEAU-CX1(T) and NEAU-JC6(T), were isolated from nymphs of stinkbug (Palomena viridissima Poda) and a beetle (Harpalus sinicus Hope), respectively, collected from Harbin, Heilongjiang Province, China. A polyphasic study was carried out to establish the taxonomic positions of these strains. The phylogenetic analysis based on 16S rRNA gene sequence of strain NEAU-CX1(T) showed it to be most closely related to Micromonospora coxensis JCM 13248(T) (99.3 % sequence similarity), Micromonospora purpureochromogenes DSM 43821(T) (99.1 %) and Micromonospora halophytica JCM 3125(T) (98.6 %), and that of strain NEAU-JC6(T) to Micromonospora haikouensis DSM 45626(T) (99.3 %), Micromonospora carbonacea JCM 3139(T) (99.1 %) and Micromonospora krabiensis JCM 12869(T) (99.1 %). The phylogenetic analysis based on gyrB gene sequence of strain NEAU-CX1(T) showed it to be most closely related to M. purpureochromogenes DSM 43821(T) (98.0 % sequence similarity), and that of strain NEAU-JC6(T) to M. haikouensis DSM 45626(T) (99.0 %) and M. carbonacea JCM 3139(T) (98.0 %). A combination of DNA-DNA hybridization results and cultural and physiological properties indicated that the two strains can be distinguished from their closest phylogenetic relatives. Thus, strains NEAU-CX1(T) and NEAU-JC6(T) represent two novel species of the genus Micromonospora, for which the names Micromonospora palomenae sp. nov. and Micromonospora harpali sp. nov. are proposed. The type strains are NEAU-CX1(T) (=CGMCC 4.7175(T) = JCM 30056(T)) and NEAU-JC6(T) (=CGMCC 4.7173(T) = JCM 30055(T)).
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People's Republic of China.
authors.forename: Baozhu
authors.initials: B
authors.lastname: Fang
authors.forename: Chongxi
authors.initials: C
authors.lastname: Liu
authors.forename: Xuejiao
authors.initials: X
authors.lastname: Guan
authors.forename: Jia
authors.initials: J
authors.lastname: Song
authors.forename: Junwei
authors.initials: J
authors.lastname: Zhao
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.forename: Chuang
authors.initials: C
authors.lastname: Li
authors.forename: Wenxi
authors.initials: W
authors.lastname: Ning
authors.forename: Xiangjing
authors.initials: X
authors.lastname: Wang
authors.forename: Wensheng
authors.initials: W
authors.lastname: Xiang
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D002681:China; D016000:Cluster Analysis; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D007313:Insecta / Q000382:microbiology*; D008847:Micromonospora / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D027081:DNA Gyrase