title: The Complete Sequence of the Acacia ligulata Chloroplast Genome Reveals a Highly Divergent clpP1 Gene.
abstract: Legumes are a highly diverse angiosperm family that include many agriculturally important species. To date, 21 complete chloroplast genomes have been sequenced from legume crops confined to the Papilionoideae subfamily. Here we report the first chloroplast genome from the Mimosoideae, Acacia ligulata, and compare it to the previously sequenced legume genomes. The A. ligulata chloroplast genome is 174,233 bp in size, comprising inverted repeats of 38,225 bp and single-copy regions of 92,798 bp and 4,985 bp [corrected]. Acacia ligulata lacks the inversion present in many of the Papilionoideae, but is not otherwise significantly different in terms of gene and repeat content. The key feature is its highly divergent clpP1 gene, normally considered essential in chloroplast genomes. In A. ligulata, although transcribed and spliced, it probably encodes a catalytically inactive protein. This study provides a significant resource for further genetic research into Acacia and the Mimosoideae. The divergent clpP1 gene suggests that Acacia will provide an interesting source of information on the evolution and functional diversity of the chloroplast Clp protease complex.
authors.affiliation: Australian Research Council Centre of Excellence in Plant Energy Biology, The University of Western Australia, Crawley, Western Australia, Australia; Botanic Gardens and Parks Authority, Kings Park and Botanic Garden, Fraser Avenue, Kings Park, Western Australia, Australia; School of Plant Biology, The University of Western Australia, Crawley, Western Australia, Australia.
authors.forename: Anna V
authors.initials: AV
authors.lastname: Williams
authors.affiliation: Australian Research Council Centre of Excellence in Plant Energy Biology, The University of Western Australia, Crawley, Western Australia, Australia; Centre of Excellence in Computational Systems Biology, The University of Western Australia, Crawley, Western Australia, Australia.
authors.forename: Laura M
authors.initials: LM
authors.lastname: Boykin
authors.affiliation: Australian Research Council Centre of Excellence in Plant Energy Biology, The University of Western Australia, Crawley, Western Australia, Australia.
authors.forename: Katharine A
authors.initials: KA
authors.lastname: Howell
authors.affiliation: Botanic Gardens and Parks Authority, Kings Park and Botanic Garden, Fraser Avenue, Kings Park, Western Australia, Australia; School of Plant Biology, The University of Western Australia, Crawley, Western Australia, Australia.
authors.forename: Paul G
authors.initials: PG
authors.lastname: Nevill
authors.affiliation: Australian Research Council Centre of Excellence in Plant Energy Biology, The University of Western Australia, Crawley, Western Australia, Australia; Centre of Excellence in Computational Systems Biology, The University of Western Australia, Crawley, Western Australia, Australia.
authors.forename: Ian
authors.initials: I
authors.lastname: Small
mesh_terms: D000045:Acacia / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D001499:Bayes Theorem; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D002736:Chloroplasts / Q000235:genetics; D014644:Genetic Variation; D054628:Genome, Chloroplast; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D012326:RNA Splicing; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D010940:Plant Proteins