title: [Effects of the Combination of Emodin and 3'-Azido-3'-Deoxythymidine on Proliferation and Apoptosis in leukemia KG-1a cells Transfected with Egr-1 siRNA].
abstract: OBJECTIVE
  This study was aimed to investigate the effects of emodin combined with 3'-azido-3'-deoxythymidine (AZT) on proliferation and apoptosis of leukemia cell line KG-1a cells and its mechanism.
  
  
  METHODS
  KG-1a cells were transfected with Egr-1 siRNA by electroporation and divided into blank control (KG-1a), nonspecific control (KG-1a/NC) and Egr-1 siRNA (KG-1a/siRNA) groups. Transfection efficiency was tested through fluorescence microscopy and flow cytometry and the transfection effect was detected by using qPCR. The cell proliferation rate was detected with MTT method. After the cells were treated with 10 µmol/L of emodin, 3200 or 1600 µmol/L of AZT and their combinations, the proliferation inhibition rates and the apoptosis rates of cells in 3 groups were detected with MTT method and FCM, respectively.
  
  
  RESULTS
  The transfection efficiency of Egr-1 siRNA was found to reach more than 59.21%; as compared with blank control(KG-1a) and nonspectic control(KG-1a/NC), the cell proliferation in Egr-1 siRNA group significantly reduced (P<0.01). The combination of emodin and AZT had considerable synergistic inhibitory effects on proliferation of normal KG-1a cells and nonspecific control(KG-1a NC) cells, but the synergistic effects disappeared after Egr-1 gene silencing.
  
  
  CONCLUSIONS
  The effects of the combination of emodin and AZT on proliferation and apoptosis may be related with Egr-1.
authors.affiliation: School of Medical Technology, Gansu College of Traditional Chinese Medicine, Lanzhou 730000, Gansu Province, China.
authors.forename: Ling-Yan
authors.initials: LY
authors.lastname: Yuan
authors.affiliation: School of Medical Technology, Gansu College of Traditional Chinese Medicine, Lanzhou 730000, Gansu Province, China. E-mail: chen72123@163.com.
authors.forename: Che
authors.initials: C
authors.lastname: Chen
authors.affiliation: School of Medical Technology, Gansu College of Traditional Chinese Medicine, Lanzhou 730000, Gansu Province, China.
authors.forename: Hui-Yuan
authors.initials: HY
authors.lastname: Chu
authors.affiliation: School of Medical Technology, Gansu College of Traditional Chinese Medicine, Lanzhou 730000, Gansu Province, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D051766:Early Growth Response Protein 1; D004642:Emodin; D005434:Flow Cytometry; D006801:Humans; D007938:Leukemia; D034741:RNA, Small Interfering; D014162:Transfection; D015215:Zidovudine
chemical_list: C497325:EGR1 protein, human; D051766:Early Growth Response Protein 1; D034741:RNA, Small Interfering; D015215:Zidovudine; D004642:Emodin