title: Lactobacillus acidophilus suppresses colitis-associated activation of the IL-23/Th17 axis.
abstract: The aim of this paper is to determine the modulatory effects of Lactobacillus acidophilus on the IL-23/Th17 immune axis in experimental colitis. DSS-induced mouse models of UC were to be saline, hormones, and different concentrations of Lactobacillus acidophilus intervention. The expression of interleukin- (IL-) 17, tumor necrosis factor α (TNFα), IL-23, transforming growth factor β1 (TGFβ1), signal transducer and activator of transcription 3 (STAT3), and phosphorylated (p)-STAT3 was examined by RT-PCR, Western blotting, and immunohistochemical analysis. And the results showed that administration of L. acidophilus suppressed Th17 cell-mediated secretion of proinflammatory cytokine IL-17 through downregulation of IL-23 and TGFβ1 expression and downstream phosphorylation of p-STAT3.
authors.affiliation: Department of Gastroenterology, Xiangya Hospital of Central South University, Changsha 410011, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Gastroenterology, Xiangya Hospital of Central South University, Changsha 410011, China.
authors.forename: Yiyou
authors.initials: Y
authors.lastname: Zou
authors.affiliation: Department of Gastroenterology, Xiangya Hospital of Central South University, Changsha 410011, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Peng
authors.affiliation: Department of Gastroenterology, Second Xiangya Hospital of Central South University, Changsha 410008, China.
authors.forename: Fanggen
authors.initials: F
authors.lastname: Lu
authors.affiliation: Department of Gastroenterology, Xiangya Hospital of Central South University, Changsha 410011, China.
authors.forename: Yani
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Department of Gastroenterology, Xiangya Hospital of Central South University, Changsha 410011, China.
authors.forename: Fujun
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Gastroenterology, Xiangya Hospital of Central South University, Changsha 410011, China.
authors.forename: Junwen
authors.initials: J
authors.lastname: Yang
mesh_terms: D000818:Animals; D003092:Colitis / Q000139:chemically induced / Q000276:immunology* / Q000628:therapy; D015536:Down-Regulation; D005260:Female; D020381:Interleukin-17 / Q000096:biosynthesis*; D053760:Interleukin-23 Subunit p19 / Q000096:biosynthesis / Q000276:immunology; D007779:Lactobacillus acidophilus / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D019936:Probiotics / Q000494:pharmacology*; D050796:STAT3 Transcription Factor / Q000096:biosynthesis; D058504:Th17 Cells / Q000276:immunology; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
chemical_list: C098680:Il17a protein, mouse; C508841:Il23a protein, mouse; D020381:Interleukin-17; D053760:Interleukin-23 Subunit p19; D050796:STAT3 Transcription Factor; C494087:Stat3 protein, mouse; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha