title: Mouse model of human RPE65 P25L hypomorph resembles wild type under normal light rearing but is fully resistant to acute light damage.
abstract: Human RPE65 mutations cause a spectrum of blinding retinal dystrophies from severe early-onset disease to milder manifestations. The RPE65 P25L missense mutation, though having <10% of wild-type (WT) activity, causes relatively mild retinal degeneration. To better understand these mild forms of RPE65-related retinal degeneration, and their effect on cone photoreceptor survival, we generated an Rpe65/P25L knock-in (KI/KI) mouse model. We found that, when subject to the low-light regime (∼100 lux) of regular mouse housing, homozygous Rpe65/P25L KI/KI mice are morphologically and functionally very similar to WT siblings. While mutant protein expression is decreased by over 80%, KI/KI mice retinae retain comparable 11-cis-retinal levels with WT. Consistently, the scotopic and photopic electroretinographic (ERG) responses to single-flash stimuli also show no difference between KI/KI and WT mice. However, the recovery of a-wave response following moderate visual pigment bleach is delayed in KI/KI mice. Importantly, KI/KI mice show significantly increased resistance to high-intensity (20 000 lux for 30 min) light-induced retinal damage (LIRD) as compared with WT, indicating impaired rhodopsin regeneration in KI/KI. Taken together, the Rpe65/P25L mutant produces sufficient chromophore under normal conditions to keep opsins replete and thus manifests a minimal phenotype. Only when exposed to intensive light is this hypomorphic mutation manifested physiologically, as its reduced expression and catalytic activity protects against the successive cycles of opsin regeneration underlying LIRD. These data also help define minimal requirements of chromophore for photoreceptor survival in vivo and may be useful in assessing a beneficial therapeutic dose for RPE65 gene therapy in humans.
authors.affiliation: Laboratory of Retinal Cell and Molecular Biology.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Laboratory of Retinal Cell and Molecular Biology.
authors.forename: Shirley
authors.initials: S
authors.lastname: Yu
authors.affiliation: Laboratory of Retinal Cell and Molecular Biology.
authors.forename: Todd
authors.initials: T
authors.lastname: Duncan
authors.affiliation: Visual Function Core and.
authors.forename: Yichao
authors.initials: Y
authors.lastname: Li
authors.affiliation: Genetic Engineering Core, National Eye Institute/NIH, Bethesda, MD, USA.
authors.forename: Pinghu
authors.initials: P
authors.lastname: Liu
authors.affiliation: Laboratory of Retinal Cell and Molecular Biology.
authors.forename: Erelda
authors.initials: E
authors.lastname: Gene
authors.affiliation: Laboratory of Retinal Cell and Molecular Biology.
authors.forename: Yoel
authors.initials: Y
authors.lastname: Cortes-Pena
authors.affiliation: Visual Function Core and.
authors.forename: Haohua
authors.initials: H
authors.lastname: Qian
authors.affiliation: Genetic Engineering Core, National Eye Institute/NIH, Bethesda, MD, USA.
authors.forename: Lijin
authors.initials: L
authors.lastname: Dong
authors.affiliation: Laboratory of Retinal Cell and Molecular Biology, redmondd@helix.nih.gov.
authors.forename: T Michael
authors.initials: TM
authors.lastname: Redmond
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D055879:Gene Knock-In Techniques; D006801:Humans; D008027:Light; D051379:Mice; D020125:Mutation, Missense; D055355:Opsins / Q000235:genetics / Q000378:metabolism; D012160:Retina / Q000378:metabolism* / Q000473:pathology; D017949:Retinal Cone Photoreceptor Cells / Q000473:pathology; D012162:Retinal Degeneration / Q000235:genetics* / Q000503:physiopathology; D012172:Retinaldehyde / Q000096:biosynthesis / Q000235:genetics*; D019745:cis-trans-Isomerases / Q000235:genetics* / Q000378:metabolism; D000099178:Retinoid Isomerohydrolase
chemical_list: D055355:Opsins; D012172:Retinaldehyde; D019745:cis-trans-Isomerases; D000099178:Retinoid Isomerohydrolase