title: [Alterations in populations of T-lymphocytes in the rats blood during experimental preeclampsia].
abstract: Immune tolerance to the fetus is predetermined mainly by HLA-G expression in trophoblasts, varying the ratio of Th1 / Th2, decrease in the content of cytotoxic T lymphocytes and natural killer cells (NK cells) and fusion "tread" of antibodies. The aim of the study was to establish the role of the placental hypoxia in regulation of expression of -lymphocytes populations in the blood of rats at different stages of pregnancy. For the purpose of modeling of PE in pregnant rats, at10-th day of gestation the lumen of the abdominal aorta below the renal artery was narrowed by the silk thread a third of its diameter (0.2 mm). In blood serum was defined the relative content of leukocyte subpopulations by indirect immunofluorescence in cytotoxic assay using monoclonal antibodies to CD4, CD8, CD14, CD16 leukocytes («ICN Pharmaceutical», USA). The study found that in the blood of animals of control group (phisiological pregnancy) within the 2nd 3rd trimester of gestation of CD4, CD8 subpopulations of lymphocytes did not change. The animals of the experimental group (pregnancy complicated by placental hypoxia) content of CD8 subpopulations and CD16 (NK cells) in the blood did not change significantly compared with those in the blood of animals in the control group, whereas CD4 T cell subpopulation in the second and in the third trimester statistical significantly decreased compared with those in control group (p<0.001). The ratio of immunoregulatory subpopulations lymphocytes CD4/CD8 decreased, number of CD14 (monocytes) phenotypes leukocytes increased. It is concluded that the placental hypoxia promotes disorder of the regulation of the immune balance of the mother's body during pregnancy, which is manifested in decrease ratio immunoregulatory subpopulations of lymphocyte, increasing the intensity of local expression of monocytes.
authors.affiliation: Tbilisi State Medical University, Georgia.
authors.forename: A
authors.initials: A
authors.lastname: Sharashenidze
authors.affiliation: Tbilisi State Medical University, Georgia.
authors.forename: L
authors.initials: L
authors.lastname: Kikalishvili
authors.affiliation: Tbilisi State Medical University, Georgia.
authors.forename: T
authors.initials: T
authors.lastname: Sanikidze
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000097:blood*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D005260:Female; D006801:Humans; D007108:Immune Tolerance; D016131:Lymphocyte Subsets / Q000276:immunology; D011225:Pre-Eclampsia / Q000097:blood* / Q000276:immunology / Q000473:pathology; D011247:Pregnancy; D051381:Rats
chemical_list: D015703:Antigens, CD