title: Effect of triptolide on expression of thrombospondin-1 and transforming growth factor-β1 in renal tubular cells.
abstract: The objective of our study is to investigate the effect of triptolide on expression of thrombospondin-1 and transforming growth factor β1 in renal tubular cells. Human renal tubular epithelial cells were stimulated by different concentrations of triptolide (0.1, 1, and 10 μg/L) in the presence of angiotensin-II (10(-7)mol/L). Real Time PCR was used to detect the mRNA expression of thrombospondin-1 and transforming growth factor β1. Western blot analysis was used to detect the protein expression. ELISA was used to detect the level of total and active transforming growth factor β1. The mRNA expression of thrombospondin-1 (3.66 ± 0.48 vs. 1.33 ± 0.26, p < 0.05) and transforming growth factor β1 (3.58 ± 0.59 vs. 1.26 ± 0.28, p < 0.05) were up-regulated obviously when stimulated by angiotensin-II. And the protein expression of thrombospondin-1 (0.5126 ± 0.0936 vs. 0.1025 ± 0.0761, p < 0.01) and transforming growth factor β1 (0.5948 ± 0.0736 vs. 0.1318 ± 0.0614, p < 0.01) were also up-regulated simultaneously when stimulated by angiotensin-II. The expression of thrombospondin-1 and transforming growth factor β1 induced by angiotensin-II were down-regulated markedly with 1 μg/L and 10 μg/L of triptolide in mRNA and protein levels (p < 0.05, p <  0.01). And triptolide (1 and 10 μg/L) could reduce the expression of total and active transforming growth factor β1 (p < 0.05, p < 0.01). In conclusion, triptolide can inhibit the expression of thrombospondin-1 and transforming growth factor β1 in mRNA and protein levels and down-regulate the levels of total and active transforming growth factor β1.
authors.affiliation: a Evidence-Based Medicine Center, School of Basic Medical Sciences, Lanzhou University , Lanzhou , Gansu , China .|b Key Laboratory of Clinical Translational Research and Evidence-Based Medicine Center of Gansu Province , Lanzhou , Gansu , China .|c The Second Clinical Medicine College of Lanzhou University , Lanzhou , Gansu , China , and.
authors.forename: Rong
authors.initials: R
authors.lastname: Zeng
authors.affiliation: a Evidence-Based Medicine Center, School of Basic Medical Sciences, Lanzhou University , Lanzhou , Gansu , China .|b Key Laboratory of Clinical Translational Research and Evidence-Based Medicine Center of Gansu Province , Lanzhou , Gansu , China .|c The Second Clinical Medicine College of Lanzhou University , Lanzhou , Gansu , China , and.
authors.forename: Lei
authors.initials: L
authors.lastname: Duan
authors.affiliation: d The First Clinical Medicine College of Lanzhou University , Lanzhou , Gansu , China.
authors.forename: Lina
authors.initials: L
authors.lastname: Sun
authors.affiliation: c The Second Clinical Medicine College of Lanzhou University , Lanzhou , Gansu , China , and.
authors.forename: Yuke
authors.initials: Y
authors.lastname: Kong
authors.affiliation: a Evidence-Based Medicine Center, School of Basic Medical Sciences, Lanzhou University , Lanzhou , Gansu , China .|b Key Laboratory of Clinical Translational Research and Evidence-Based Medicine Center of Gansu Province , Lanzhou , Gansu , China .
authors.forename: Kehu
authors.initials: K
authors.lastname: Yang
mesh_terms: D000704:Analysis of Variance; D015153:Blotting, Western; D049109:Cell Proliferation / Q000235:genetics; D002470:Cell Survival; D002478:Cells, Cultured / Q000378:metabolism; D004224:Diterpenes / Q000378:metabolism*; D015536:Down-Regulation; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells; D004852:Epoxy Compounds / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007687:Kidney Tubules, Proximal / Q000166:cytology / Q000378:metabolism*; D008297:Male; D010616:Phenanthrenes / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D012016:Reference Values; D019700:Thrombospondin 1 / Q000235:genetics*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
chemical_list: D004224:Diterpenes; D004852:Epoxy Compounds; D010616:Phenanthrenes; D012333:RNA, Messenger; D019700:Thrombospondin 1; D053773:Transforming Growth Factor beta1; C001899:triptolide
keywords: Angiotensin-II; renal tubular cells; renal tubular interstitium; thrombospondin 1; transforming growth factor β1; triptolide