title: Inhibition of Nonsmall Cell Lung Cancer Cell Migration by Protein Arginine Methyltransferase 1-small Hairpin RNA Through Inhibiting Epithelial-mesenchymal Transition, Extracellular Matrix Degradation, and Src Phosphorylation In Vitro.
abstract: BACKGROUND
  Protein arginine methyltransferases 1 (PRMT1) is over-expressed in a variety of cancers, including lung cancer, and is correlated with a poor prognosis of tumor development. This study aimed to investigate the role of PRMT1 in nonsmall cell lung cancer (NSCLC) migration in vitro.
  
  
  METHODS
  In this study, PRMT1 expression in the NSCLC cell line A549 was silenced using lentiviral vector-mediated short hairpin RNAs. Cell migration was measured using both scratch wound healing and transwell cell migration assays. The mRNA expression levels of matrix metalloproteinase 2 (MMP-2) and tissue inhibitor of metalloproteinase 1, 2 (TIMP1, 2) were measured using quantitative real-time reverse transcription-polymerase chain reaction. The expression levels of protein markers for epithelial-mesenchymal transition (EMT) (E-cadherin, N-cadherin), focal adhesion kinase (FAK), Src, AKT, and their corresponding phosphorylated states were detected by Western blot.
  
  
  RESULTS
  Cell migration was significantly inhibited in the PRMT1 silenced group compared to the control group. The mRNA expression of MMP-2 decreased while TIMP1 and TIMP2 increased significantly. E-cadherin mRNA expression also increased while N-cadherin decreased. Only phosphorylated Src levels decreased in the silenced group while FAK or AKT remained unchanged.
  
  
  CONCLUSIONS
  PRMT1-small hairpin RNA inhibits the migration abilities of NSCLC A549 cells by inhibiting EMT, extracellular matrix degradation, and Src phosphorylation in vitro.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Research Center, First Affiliated Hospital of Huzhou University, Huzhou, Zhejiang 313000, China.
authors.forename: Ge
authors.initials: G
authors.lastname: Cui
authors.forename: Yun-Liang
authors.initials: YL
authors.lastname: Yao
authors.forename: Yue
authors.initials: Y
authors.lastname: Guo
authors.forename: Qi-Chun
authors.initials: QC
authors.lastname: Wang
authors.forename: Xi-Ning
authors.initials: XN
authors.lastname: Li
authors.forename: Wen-Ming
authors.initials: WM
authors.lastname: Feng
mesh_terms: D015153:Blotting, Western; D002289:Carcinoma, Non-Small-Cell Lung / Q000201:enzymology* / Q000235:genetics; D002460:Cell Line; D002465:Cell Movement / Q000235:genetics / Q000502:physiology*; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics / Q000502:physiology*; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D006801:Humans; D011484:Protein-Arginine N-Methyltransferases / Q000235:genetics* / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics / Q000502:physiology
chemical_list: D016326:Extracellular Matrix Proteins; D034741:RNA, Small Interfering; D011484:Protein-Arginine N-Methyltransferases