title: Investigations into cytotoxic effects of the herbal preparation Abnormal Savda Munziq.
abstract: OBJECTIVE
  To evaluate the effects of Abnormal Savda Munziq (ASMq), a traditional herbal medicine, for the prevention and treatment of human diseases, e.g. bowel cancer.
  
  
  METHODS
  The parameters total polyphenol content, cell proliferation and DNA-damage as well as RNA and protein-oxidation were analysed in vitro. Besides, the expressions of miRNA and tumor suppressor genes as well as cellular senescence were evaluated.
  
  
  RESULTS
  ASMq had a high polyphenol content and induced damage to proteins, RNA as well as to DNA, which is correlated with its cytotoxicity. Furthermore ASMq up-regulated the tumor suppressor genes p21, p53 and p16 and down-regulated the micro-RNAs hsa-mir-17 and hsa-mir-106b. In addition cellular growth arrest and SA-β-gal-staining were induced.
  
  
  CONCLUSIONS
  ASMq has the ability to induce DNA damage and cellular senescence, which are double-edged mechanisms in fighting cancer, as they might also have harmful side effects.
authors.affiliation: Department of Biomedical Sciences, Institute of Physiology, Pathophysiology and Biophysics, University of Veterinary Medicine Vienna, Vienna, A-1210, Austria.
authors.forename: Harald
authors.initials: H
authors.lastname: Kühnel
authors.forename: Abulimiti
authors.initials: A
authors.lastname: Adilijiang
authors.forename: Agnes
authors.initials: A
authors.lastname: Dadak
authors.forename: Matthias
authors.initials: M
authors.lastname: Wieser
authors.forename: Halmurat
authors.initials: H
authors.lastname: Upur
authors.forename: Klaus
authors.initials: K
authors.lastname: Stolze
authors.forename: Johannes
authors.initials: J
authors.lastname: Grillari
authors.forename: Alois
authors.initials: A
authors.lastname: Strasser