title: Cariostatic effect of green tea in comparison with common anticariogenic agents: an in vitro study.
abstract: Background and aims. Anticariogenic effects of different mouthrinses have been shown previously. In this in vitro study the anticariogenic effects of polyphenol extract of green tea with 0.05% fluoride, 0.2% chlorhexidine and fluoride-chlorhexidine were compared. Materials and methods. This in vitro study was performed on 50 maxillary premolars in 5 groups: 1) normal saline; 2) a 10% solution of green tea polyphenol extract; 3) 0.05% fluoride; 4) 0.2% chlorhexidine; and 5) fluoride-chlorhexidine. Each tooth was placed in a tube which contained a cariogenic solution. Every day the teeth were washed (depending on the experimental groups) with 5 mL of mouthrinse solution. The depth of the caries was measured under a polarized light microscope. Data were analyzed using SPSS 13.0 with Kolmogorov-Smirnov, one-way ANOVA and Tukey tests. Results. The mean and standard deviation (in µm) of caries depth were 194±16.43, 175±17.94, 142±9.34, 155±13.27, and 144±8.57 in groups 1 to 5, respectively, with significant differences between the groups (P<0.001). Tukey test showed that although there was no significant difference in the depth of caries in groups 1 and 2 (P>0.001), they were significantlyless than those in groups 3 to 5 (P<0.001). There was no significant difference between decay depth of groups 3, 4 and 5 (P>0.001). Conclusion. The anticariogenic effect of fluoride-chlorhexidine was the highest among the groups. Although green tea showed higher cariostatic effects than normal saline, in comparison with other mouthrinses, it is less effective. More re-search is strongly recommended for clinical use of green tea as an anticariogenic agent.
authors.affiliation: Assistant Professor, Department of Oral Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Mina
authors.initials: M
authors.lastname: Jazaeri
authors.affiliation: Assistant Professor, Department of Oral Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Pakdek
authors.affiliation: Associate Professor, Department of Operative Dentistry, Member of Dental Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Loghman
authors.initials: L
authors.lastname: Rezaei-Soufi
authors.affiliation: Associate Professor, Department of Oral Medicine, Member of Dental Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Hamidreza
authors.initials: H
authors.lastname: Abdolsamadi
authors.affiliation: Assistant Professor, Department of Oral Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Nasrin
authors.initials: N
authors.lastname: Rafieian
keywords: Cariostatic agent; dental caries; green tea; mouthwash; polyphenols