title: Bax inhibitor-1 is overexpressed in non-small cell lung cancer and promotes its progression and metastasis.
abstract: Bax inhibitor-1 (BI-1) is a novel anti-apoptotic protein. While the effect of BI-1 on apoptosis has been extensively studied, less is known about how BI-1 is related to oncogenesis and the progression, particularly, the invasion and metastasis of cancer. In this study, we investigated the expression and function of BI-1 in human non-small cell lung cancer (NSCLC). RT-PCR and immunohistochemistry were performed on clinical NSCLC samples to detect BI-1 expression. Northern blot and Western blot analysis were performed to detect BI-1 expression of in NSCLC cell lines. Cell proliferation and apoptosis were analyzed by flow cytometry. Our results showed that the overexpression of BI-1 was significantly related to oncogenesis of NSCLC (P < 0.05). Meanwhile, we identified a novel 5'end from normal lung-derived BI-1 transcript, different from any transcript deposit in the Genbank, but we detected no mutations in the coding sequence and the promoter region of BI-1 and no abnormal splicing of the alternative first exon. Ectopic expression of BI-1 changed the proliferation and apoptosis of AGZY83-a and Anip973 cells. In conclusion, BI-1 is overexpressed in NSCLC and promotes the progression and metastasis of NSCLC.
authors.affiliation: Department of Ophthalmology, The First Clinical Hospital Affiliated to Harbin Medical University Harbin 150001, Heilongjiang, China.
authors.forename: Bingjie
authors.initials: B
authors.lastname: Lu
authors.affiliation: Department of Genetics and Cellular Biology, Harbin Medical University Harbin 150086, Heilongjiang, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Genetics and Cellular Biology, Harbin Medical University Harbin 150086, Heilongjiang, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Genetics and Cellular Biology, Harbin Medical University Harbin 150086, Heilongjiang, China.
authors.forename: Yingmei
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Genetics and Cellular Biology, Harbin Medical University Harbin 150086, Heilongjiang, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Genetics and Cellular Biology, Harbin Medical University Harbin 150086, Heilongjiang, China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Ren
authors.affiliation: Department of Genetics and Cellular Biology, Harbin Medical University Harbin 150086, Heilongjiang, China.
authors.forename: Songbin
authors.initials: S
authors.lastname: Fu
authors.affiliation: Department of Thyroid and Breast Diseases, The First Clinical Hospital Affiliated to Harbin Medical University Harbin 150001, Heilongjiang, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D000328:Adult; D017209:Apoptosis / Q000502:physiology; D051017:Apoptosis Regulatory Proteins / Q000032:analysis / Q000096:biosynthesis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D002289:Carcinoma, Non-Small-Cell Lung / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018450:Disease Progression; D005434:Flow Cytometry; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000473:pathology*; D008297:Male; D008565:Membrane Proteins / Q000032:analysis / Q000096:biosynthesis*; D009361:Neoplasm Invasiveness / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D051017:Apoptosis Regulatory Proteins; D014408:Biomarkers, Tumor; D008565:Membrane Proteins; C484209:TMBIM6 protein, human
keywords: Bax inhibitor-1; apoptosis; metastasis; non-small cell lung cancer