title: Molecular diagnosis of toxoplasmosis: value of the buffy coat for the detection of circulating Toxoplasma gondii.
abstract: Early detection of Toxoplasma tachyzoites circulating in blood using PCR is recommended for immunosuppressed patients at high risk for disseminated toxoplasmosis. Using a toxoplasmosis mouse model, we show that the sensitivity of detection is higher using buffy coat isolated from a large blood volume than using whole blood for this molecular monitoring.
authors.affiliation: Laboratoire de Parasitologie et Mycologie, CHU de Grenoble et Université Grenoble Alpes, Grenoble 1 BP 217, 38043 Grenoble, France; Pôle "Biologie Moléculaire" du Centre National de Référence de la Toxoplasmose, Montpellier, France. Electronic address: MPBrenierPinchart@chu-grenoble.fr.
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Brenier-Pinchart
authors.affiliation: Laboratoire de Parasitologie et Mycologie, CHU de Grenoble et Université Grenoble Alpes, Grenoble 1 BP 217, 38043 Grenoble, France.
authors.forename: Elodie
authors.initials: E
authors.lastname: Capderou
authors.affiliation: Laboratoire de Parasitologie et Mycologie, CHU de Grenoble et Université Grenoble Alpes, Grenoble 1 BP 217, 38043 Grenoble, France.
authors.forename: Rose-Laurence
authors.initials: RL
authors.lastname: Bertini
authors.affiliation: Laboratoire de Parasitologie et Mycologie, CHU de Grenoble et Université Grenoble Alpes, Grenoble 1 BP 217, 38043 Grenoble, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Bailly
authors.affiliation: Laboratoire de Parasitologie et Mycologie, CHU de Grenoble et Université Grenoble Alpes, Grenoble 1 BP 217, 38043 Grenoble, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Fricker-Hidalgo
authors.affiliation: Pôle "Biologie Moléculaire" du Centre National de Référence de la Toxoplasmose, Montpellier, France; Laboratoire de Parasitologie-Mycologie, CHU de Montpellier, CNRS UMR5290/IRD 224/UM1 ("MiVEGEC"), University of Montpellier 1, Montpellier, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Varlet-Marie
authors.affiliation: Laboratoire de Parasitologie et Mycologie, CHU de Grenoble et Université Grenoble Alpes, Grenoble 1 BP 217, 38043 Grenoble, France.
authors.forename: Jean-Benjamin
authors.initials: JB
authors.lastname: Murat
authors.affiliation: Pôle "Biologie Moléculaire" du Centre National de Référence de la Toxoplasmose, Montpellier, France; Laboratoire de Parasitologie-Mycologie, CHU de Montpellier, CNRS UMR5290/IRD 224/UM1 ("MiVEGEC"), University of Montpellier 1, Montpellier, France.
authors.forename: Yvon
authors.initials: Y
authors.lastname: Sterkers
authors.affiliation: Pôle "Biologie Moléculaire" du Centre National de Référence de la Toxoplasmose, Montpellier, France; Laboratoire de Parasitologie-Mycologie, Pôle de Biologie et Pathologie Médicale, CHU la Pité Salpêtrière, Charles Foix 47, bd de l'Hôpital, 75651 Paris Cedex 13, France.
authors.forename: Fériel
authors.initials: F
authors.lastname: Touafek
authors.affiliation: Pôle "Biologie Moléculaire" du Centre National de Référence de la Toxoplasmose, Montpellier, France; Laboratoire de Parasitologie-Mycologie, CHU de Montpellier, CNRS UMR5290/IRD 224/UM1 ("MiVEGEC"), University of Montpellier 1, Montpellier, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Bastien
authors.affiliation: Laboratoire de Parasitologie et Mycologie, CHU de Grenoble et Université Grenoble Alpes, Grenoble 1 BP 217, 38043 Grenoble, France; Pôle "Biologie Moléculaire" du Centre National de Référence de la Toxoplasmose, Montpellier, France.
authors.forename: Hervé
authors.initials: H
authors.lastname: Pelloux
mesh_terms: D000818:Animals; D058621:Blood Buffy Coat / Q000469:parasitology*; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D051379:Mice; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D010274:Parasitology / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014122:Toxoplasma / Q000235:genetics / Q000302:isolation & purification*; D014123:Toxoplasmosis / Q000175:diagnosis* / Q000469:parasitology
keywords: Buffy coat; Diagnosis; PCR; Toxoplasma gondii; Toxoplasmosis; Whole blood