title: Umbilical Cord Mesenchymal Stem Cells Combined With a Collagenfibrin Double-layered Membrane Accelerates Wound Healing.
abstract: The aim of this study was to examine the effects of human umbilical cord mesenchymal stem cells (hUCMSCs) in combination with a collagen-fibrin double-layered membrane on wound healing in mice. A collagen-fibrin double-layered membrane was prepared, and the surface properties of the support material were investigated using a scanning electron microscope. Twenty-four mice were prepared for use as full-thickness skin wound models and randomly divided into 3 groups: group A, a control group in which the wounds were bound using a conventional method; group B, a group treated with hUCMSCs combined with a collagen membrane; and group C, a group treated with hUCMSCs combined with a collagen-fibrin double-layered membrane. The postoperative concrescence of the wounds was observed daily to evaluate the effects of the different treatments. Scanning electron microscope observation showed the collagen-fibrin scaffolds exhibited a highly porous and interconnected structure, and wound healing in the double-layered membrane group was better than in groups A or B. Treatment with hUCMSCs combined with a collagen-fibrin double-layered membrane accelerated wound healing.
authors.affiliation: Department of Life Sciences and Technology, Xinxiang Medical University, Henan, China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Nan
authors.affiliation: Sanquan Medical College, Xinxiang Medical University, Henan, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.affiliation: Department of Life Sciences and Technology, Xinxiang Medical University, Henan, China.
authors.forename: Hongli
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Life Sciences and Technology, Xinxiang Medical University, Henan, China.
authors.forename: Zhihao
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of Life Sciences and Technology, Xinxiang Medical University, Henan, China.
authors.forename: Jiannan
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Life Sciences and Technology, Xinxiang Medical University, Henan, China.
authors.forename: Manman
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Life Sciences and Technology, Xinxiang Medical University, Henan, China; email: wenbinnancn@126.com.
authors.forename: Zhiqing
authors.initials: Z
authors.lastname: Yuan
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism*; D020405:Dermis / Q000098:blood supply / Q000293:injuries / Q000473:pathology*; D004195:Disease Models, Animal; D005337:Fibrin / Q000378:metabolism*; D048091:Guided Tissue Regeneration; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology; D051379:Mice; D018919:Neovascularization, Physiologic; D012867:Skin / Q000473:pathology*; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014945:Wound Healing; D014947:Wounds and Injuries / Q000473:pathology*
chemical_list: D005337:Fibrin; D003094:Collagen