title: Development of a conjugate vaccine against invasive pneumococcal disease based on capsular polysaccharides coupled with PspA/family 1 protein of Streptococcus pneumoniae.
abstract: The efforts were focused on exploring alternative pneumococcal vaccine strategies, aimed at addressing the shortcomings of existing formulations, without compromising efficacy. Our strategy involved the use of the carrier protein, pneumococcal surface protein A (PspA), conjugated with capsular polysaccharides (CPS), to provide effective and non-serotype-dependent protection. In this study, we generated a stable Escherichia coli construct expressing functional PspA from a capsular serotype 6B strain and confirmed it belonging to family 1, which was conjugated with CPS. The distribution of anti-CPS antibody response was almost completely of IgG2a subclass followed by IgG3 and low level of IgG1 subclass, but that of anti-PspA IgG subclass antibodies was almost equal IgG1 and IgG2a subclasses. Though PspA was less conspicuous on the surface of pneumococci than the capsule, the antibodies induced with CPS-rPspA conjugate possessed more accessibility to the surface of Streptococcus pneumoniae serotype 6B and 19F (the same family 1 PspA). By survival experiment, the result suggested that the level of cross-protection after immunized with the conjugate was more measurable within the same family 1. The CPS-rPspA conjugate not only induced CPS-specific protection but also provided PspA specific cross-protection.
authors.affiliation: College of Biological Science and Biotechnology, Fuzhou University, Fuzhou, Fujian 350002, China. Electronic address: haiylin@163.com.
authors.forename: Haiying
authors.initials: H
authors.lastname: Lin
authors.affiliation: College of Biological Science and Biotechnology, Fuzhou University, Fuzhou, Fujian 350002, China. Electronic address: 59835419@qq.com.
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Peng
authors.affiliation: College of Biological Science and Biotechnology, Fuzhou University, Fuzhou, Fujian 350002, China. Electronic address: haiyss@yahoo.com.cn.
authors.forename: ZiLin
authors.initials: Z
authors.lastname: Lin
authors.affiliation: College of Biological Science and Biotechnology, Fuzhou University, Fuzhou, Fujian 350002, China. Electronic address: 1737823746@qq.com.
authors.forename: Shuangling
authors.initials: S
authors.lastname: Zhang
authors.affiliation: College of Biological Science and Biotechnology, Fuzhou University, Fuzhou, Fujian 350002, China.
authors.forename: Yanghao
authors.initials: Y
authors.lastname: Guo
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D056738:Cross Protection; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D007074:Immunoglobulin G / Q000097:blood; D008807:Mice, Inbred BALB C; D011008:Pneumococcal Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D022242:Pneumococcal Vaccines / Q000008:administration & dosage / Q000276:immunology* / Q000302:isolation & purification; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D013296:Streptococcus pneumoniae / Q000276:immunology*; D016019:Survival Analysis; D018074:Vaccines, Conjugate / Q000008:administration & dosage / Q000276:immunology / Q000302:isolation & purification; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D007074:Immunoglobulin G; D022242:Pneumococcal Vaccines; D011135:Polysaccharides, Bacterial; D011994:Recombinant Proteins; D018074:Vaccines, Conjugate; D014614:Vaccines, Synthetic; C051592:pneumococcal surface protein A
keywords: Capsular polysaccharides; Conjugate; Family 1; PspA; Streptococcus pneumoniae