title: Lipid droplets formation in human endothelial cells in response to polyunsaturated fatty acids and 1-methyl-nicotinamide (MNA); confocal Raman imaging and fluorescence microscopy studies.
abstract: In this work the formation of lipid droplets (LDs) in human endothelial cells culture in response to the uptake of polyunsaturated fatty acids (PUFAs) was studied. Additionally, an effect of 1-methylnicotinamide (MNA) on the process of LDs formation was investigated. LDs have been previously described structurally and to some degree biochemically, however neither the precise function of LDs nor the factors responsible for LD induction have been clarified. Lipid droplets, sometimes referred in the literature as lipid bodies are organelles known to regulate neutrophil, eosinophil, or tumor cell functions but their presence and function in the endothelium is largely unexplored. 3D linear Raman spectroscopy was used to study LDs formation in vitro in a single endothelial cell. The method provides information about distribution and size of LDs as well as their composition. The incubation of endothelial cells with various PUFAs resulted in formation of LDs. As a complementary method for LDs identification a fluorescence microscopy was applied. Fluorescence measurements confirmed the Raman results suggesting endothelial cells uptake of PUFAs and subsequent LDs formation in the cytoplasm of the endothelium. Furthermore, MNA seem to potentiate intracellular uptake of PUFAs to the endothelium that may bear physiological and pharmacological significance. Confocal Raman imaging of HAoEC cell with LDs.
authors.affiliation: Jagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University, Bobrzynskiego 14, 30-348, Krakow, Poland.|Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060, Krakow, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Majzner
authors.affiliation: Jagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University, Bobrzynskiego 14, 30-348, Krakow, Poland. stefan.chlopicki@jcet.eu.|Department of Experimental Pharmacology, Chair of Pharmacology, Jagiellonian University, Grzegorzecka 16, 31-531, Krakow, Poland. stefan.chlopicki@jcet.eu.
authors.forename: Stefan
authors.initials: S
authors.lastname: Chlopicki
authors.affiliation: Jagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University, Bobrzynskiego 14, 30-348, Krakow, Poland. baranska@chemia.uj.edu.pl.|Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060, Krakow, Poland. baranska@chemia.uj.edu.pl.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Baranska
mesh_terms: D001011:Aorta / Q000166:cytology; D016718:Arachidonic Acid / Q000378:metabolism / Q000494:pharmacology; D001692:Biological Transport; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D015118:Eicosapentaenoic Acid / Q000378:metabolism / Q000494:pharmacology; D042783:Endothelial Cells / Q000166:cytology*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D066292:Lipid Droplets / Q000187:drug effects / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D009536:Niacinamide / Q000031:analogs & derivatives* / Q000494:pharmacology; D013059:Spectrum Analysis, Raman
chemical_list: D005231:Fatty Acids, Unsaturated; D009536:Niacinamide; D016718:Arachidonic Acid; D015118:Eicosapentaenoic Acid; C024058:N(1)-methylnicotinamide
keywords: Raman spectroscopy; arachidonic acid; cluster analysis; endothelium; fluorescence; lipid droplets; polyunsaturated fatty acids