title: Novel spectrophotometric method for detection and estimation of butanol in acetone-butanol-ethanol fermenter.
abstract: A new, simple, rapid and selective spectrophotometric method has been developed for detection and estimation of butanol in fermentation broth. The red colored compound, produced during reduction of diquat-dibromide-monohydrate with 2-mercaptoethanol in aqueous solution at high pH (>13), becomes purple on phase transfer to butanol and gives distinct absorption at λ520nm. Estimation of butanol in the fermentation broth has been performed by salting out extraction (SOE) using saturated K3PO4 solution at high pH (>13) followed by absorbance measurement using diquat reagent. Compatibility and optimization of diquat reagent concentration for detection and estimation of butanol concentration in the fermentation broth range was verified by central composite design. A standard curve was constructed to estimate butanol in acetone-ethanol-butanol (ABE) mixture under optimized conditions. The spectrophotometric results for butanol estimation, was found to have 87.5% concordance with the data from gas chromatographic analysis.
authors.affiliation: Institut National de la Recherche Scientifique, Centre-Eau Terre Environnement, 490, Rue de la Couronne, Québec (QC), Canada G1K 9A9.
authors.forename: Sampa
authors.initials: S
authors.lastname: Maiti
authors.affiliation: Institut National de la Recherche Scientifique, Centre-Eau Terre Environnement, 490, Rue de la Couronne, Québec (QC), Canada G1K 9A9.
authors.forename: Saurabh Jyoti
authors.initials: SJ
authors.lastname: Sarma
authors.affiliation: Institut National de la Recherche Scientifique, Centre-Eau Terre Environnement, 490, Rue de la Couronne, Québec (QC), Canada G1K 9A9. Electronic address: satinder.brar@ete.inrs.ca.
authors.forename: Satinder Kaur
authors.initials: SK
authors.lastname: Brar
authors.affiliation: Centre de Recherche Industrielle du Québec (CRIQ), Québec (QC), Canada.
authors.forename: Yann Le
authors.initials: YL
authors.lastname: Bihan
authors.affiliation: Institut National de la Recherche Scientifique, Centre-Eau Terre Environnement, 490, Rue de la Couronne, Québec (QC), Canada G1K 9A9.
authors.forename: Patrick
authors.initials: P
authors.lastname: Drogui
authors.affiliation: Centre de Recherche Industrielle du Québec (CRIQ), Québec (QC), Canada.
authors.forename: Gerardo
authors.initials: G
authors.lastname: Buelna
authors.affiliation: CO2 Solutions Inc., 2300, rue Jean-Perrin, Québec, Québec, Canada G2C 1T9.
authors.forename: Mausam
authors.initials: M
authors.lastname: Verma
authors.affiliation: Bioprocess Engineering and Biotechnology Department, Federal University of Paraná, Centro Politécnico, Usina Piloto B, CEP 81531-990 Curitiba, Paraná, Brazil.
authors.forename: Carlos Ricardo
authors.initials: CR
authors.lastname: Soccol
mesh_terms: D000096:Acetone / Q000378:metabolism*; D000440:Butanols / Q000032:analysis* / Q000378:metabolism; D046970:Clostridium beijerinckii / Q000254:growth & development / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D013053:Spectrophotometry / Q000379:methods*
chemical_list: D000440:Butanols; D000096:Acetone; D000431:Ethanol
keywords: Butanol estimation; Diquat reagent; Real fermenter; Salting out extraction (SOE); Spectrophotometric method