title: Simultaneous extraction and quantification of lamotrigine, phenobarbital, and phenytoin in human plasma and urine samples using solidified floating organic drop microextraction and high-performance liquid chromatography.
abstract: A novel and simple method based on solidified floating organic drop microextraction followed by high-performance liquid chromatography with ultraviolet detection has been developed for simultaneous preconcentration and determination of phenobarbital, lamotrigine, and phenytoin in human plasma and urine samples. Factors affecting microextraction efficiency such as the type and volume of the extraction solvent, sample pH, extraction time, stirring rate, extraction temperature, ionic strength, and sample volume were optimized. Under the optimum conditions (i.e. extraction solvent, 1-undecanol (40 μL); sample pH, 8.0; temperature, 25°C; stirring rate, 500 rpm; sample volume, 7 mL; potassium chloride concentration, 5% and extraction time, 50 min), the limits of detection for phenobarbital, lamotrigine, and phenytoin were 1.0, 0.1, and 0.3 μg/L, respectively. Also, the calibration curves for phenobarbital, lamotrigine, and phenytoin were linear in the concentration range of 2.0-300.0, 0.3-200.0, and 1.0-200.0 μg/L, respectively. The relative standard deviations for six replicate extractions and determinations of phenobarbital, lamotrigine, and phenytoin at 50 μg/L level were less than 4.6%. The method was successfully applied to determine phenobarbital, lamotrigine, and phenytoin in plasma and urine samples.
authors.affiliation: Department of Chemistry, Faculty of Science, Yazd University, Yazd, Iran.|Khorassan Research Institute for Food Science and Technology, Mashhad, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Asadi
authors.affiliation: Department of Chemistry, Faculty of Science, Yazd University, Yazd, Iran.
authors.forename: Shayessteh
authors.initials: S
authors.lastname: Dadfarnia
authors.affiliation: Department of Chemistry, Faculty of Science, Yazd University, Yazd, Iran.
authors.forename: Ali Mohammad
authors.initials: AM
authors.lastname: Haji Shabani
authors.affiliation: Khorassan Research Institute for Food Science and Technology, Mashhad, Iran.
authors.forename: Bijan
authors.initials: B
authors.lastname: Abbasi
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006863:Hydrogen-Ion Concentration; D000077213:Lamotrigine; D057230:Limit of Detection; D059627:Liquid Phase Microextraction; D009930:Organic Chemicals; D009994:Osmolar Concentration; D010634:Phenobarbital / Q000097:blood* / Q000652:urine*; D010672:Phenytoin / Q000097:blood* / Q000652:urine*; D015203:Reproducibility of Results; D052617:Solid Phase Microextraction; D012997:Solvents; D013696:Temperature; D014227:Triazines / Q000097:blood* / Q000652:urine*
chemical_list: D009930:Organic Chemicals; D012997:Solvents; D014227:Triazines; D010672:Phenytoin; D000077213:Lamotrigine; D010634:Phenobarbital
keywords: High-performance liquid chromatography; Lamotrigine; Phenobarbital; Phenytoin; Solidified floating organic drop microextraction