title: Ceftibuten-containing agar plate for detecting group B streptococci with reduced penicillin susceptibility (PRGBS).
abstract: Penicillins remain first-line agents for treatment of group B Streptococcus (Streptococcus agalactiae; GBS) infections; however, several reports have confirmed the existence of GBS with reduced penicillin susceptibility (PRGBS). Because no selective agar plates for detection of PRGBS are available to date, in this investigation, we developed the selective agar plate for detection of PRGBS. We used 19 genetically well-confirmed PRGBS isolates and 38 penicillin-susceptible GBS isolates identified in Japan. For preparation of trial PRGBS-selective agar plates, we added 1 of antimicrobial agents (among oxacillin, ceftizoxime, and ceftibuten) to a well-established GBS-selective agar plate. Among 12 trial PRGBS-selective agar plates, Muller-Hinton agar containing 128 μg/mL ceftibuten with 5% sheep blood, 8 μg/mL gentamicin, and 12 μg/mL nalidixic acid was the most appropriate selective agar for PRGBS, showing 100% sensitivity and 81.6% specificity. In cases of potential nosocomial spread of PRGBS, the selective agar plate could be useful and reliable.
authors.affiliation: Department of Bacteriology, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466, Japan.
authors.forename: Chitose
authors.initials: C
authors.lastname: Kamiya
authors.affiliation: Department of Bacteriology, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466, Japan. Electronic address: koujikim@med.nagoya-u.ac.jp.
authors.forename: Kouji
authors.initials: K
authors.lastname: Kimura
authors.affiliation: Department of Bacteriology, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466, Japan.
authors.forename: Yo
authors.initials: Y
authors.lastname: Doyama
authors.affiliation: Department of Bacteriology, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Miyazaki
authors.affiliation: Department of Bacteriology, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466, Japan.
authors.forename: Makiko
authors.initials: M
authors.lastname: Morimoto
authors.affiliation: Department of Bacteriology, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466, Japan.
authors.forename: Hirotsugu
authors.initials: H
authors.lastname: Banno
authors.affiliation: Department of Health and Medical Sciences, Shinshu University Graduate School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan.
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Nagano
authors.affiliation: Department of Bacteriology, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466, Japan.
authors.forename: Wanchun
authors.initials: W
authors.lastname: Jin
authors.affiliation: Department of Bacteriology, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466, Japan.
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Wachino
authors.affiliation: Department of Bacteriology, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Yamada
authors.affiliation: Department of Bacteriology, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466, Japan.
authors.forename: Yoshichika
authors.initials: Y
authors.lastname: Arakawa
mesh_terms: D000362:Agar; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001431:Bacteriological Techniques / Q000379:methods*; D000077722:Ceftibuten; D002511:Cephalosporins / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry*; D024881:Drug Resistance, Bacterial; D005839:Gentamicins / Q000378:metabolism; D006801:Humans; D007564:Japan; D009268:Nalidixic Acid / Q000378:metabolism; D012680:Sensitivity and Specificity; D013290:Streptococcal Infections / Q000175:diagnosis*; D013292:Streptococcus agalactiae / Q000187:drug effects / Q000302:isolation & purification*
chemical_list: D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D003470:Culture Media; D005839:Gentamicins; D009268:Nalidixic Acid; D000362:Agar; D000077722:Ceftibuten
keywords: Group B Streptococcus; Group B streptococci with reduced penicillin susceptibility; PRGBS; Selective agar plate; Streptococcus agalactiae