title: Nephroblastoma overexpressed gene (NOV) enhances RCC cell motility through upregulation of ICAM-1 and COX-2 expression via Akt pathway.
abstract: Renal cell carcinoma (RCC) carries a high risk of malignancy and metastasis. The inducible isoform of prostaglandin synthase, cyclooxygenase (COX)-2, and ICAM-1 may be involved in tumor metastasis. CCN3, also called nephroblastoma overexpressed gene (NOV), has been found to regulate the proliferation and differentiation of cancer cells. The effects of NOV on RCC cell migration and expression of COX-2 and ICAM-1 have not described yet in detail. But here, NOV was found to promote the migration and expression of COX-2 and ICAM-1 in human RCC cells. Akt inhibitor was found to interfere with this NOV-induced migration and up-regulation of COX-2 and ICAM-1 in RCC cells. NOV stimulation was here found to promote the phosphorylation of Akt. RCC tissue chips were subjected to IHC staining, which showed COX-2 expression in RCC tissues to be a significantly closely correlated with NOV expression, with significance determined using Pearson correlation testing (P < 0.05). The results of the current work indicate that NOV activates COX-2 and ICAM-1 through Akt, promoting the migration of RCC cells.
authors.affiliation: Department of Urology, Shandong Provincial Hospital Affiliated to Shandong University Jinan 250021, P. R. China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of neurology, Qianfoshan Hospital Affiliated to Shandong University Jinan, People's Republic of China.
authors.forename: Liping
authors.initials: L
authors.lastname: Han
authors.affiliation: Shandong University Jinan 250000, P. R. China.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Urology, Shandong Provincial Hospital Affiliated to Shandong University Jinan 250021, P. R. China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Urology, Shandong Provincial Hospital Affiliated to Shandong University Jinan 250021, P. R. China.
authors.forename: Sentai
authors.initials: S
authors.lastname: Ding
authors.affiliation: Department of Urology, Shandong Provincial Hospital Affiliated to Shandong University Jinan 250021, P. R. China.
authors.forename: Jiaju
authors.initials: J
authors.lastname: Lu
authors.affiliation: Department of Urology, Shandong Provincial Hospital Affiliated to Shandong University Jinan 250021, P. R. China.
authors.forename: Dongbin
authors.initials: D
authors.lastname: Bi
authors.affiliation: West China School of Public Health, Sichuan University Chengdu, Sichuan, P. R. China.
authors.forename: Yikun
authors.initials: Y
authors.lastname: Mei
authors.affiliation: Department of Urology, Shandong Provincial Hospital Affiliated to Shandong University Jinan 250021, P. R. China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Niu
mesh_terms: D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism*; D007680:Kidney Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D055514:Nephroblastoma Overexpressed Protein / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D015854:Up-Regulation
chemical_list: C526319:CCN3 protein, human; D055514:Nephroblastoma Overexpressed Protein; D018799:Intercellular Adhesion Molecule-1; D051546:Cyclooxygenase 2; D051057:Proto-Oncogene Proteins c-akt
keywords: COX-2; ICAM-1; NOV; akt pathway; cell motility; renal cell carcinoma