title: Ginsenoside Rg3 bile salt-phosphatidylcholine-based mixed micelles: design, characterization, and evaluation.
abstract: 20(R)-Ginsenoside Rg3 (G-Rg3) has good inhibition of tumor angiogenesis and anti-tumor effect. However, its poor aqueous solubility and liposolubility are not ideal for clinical applications. In this study, a G-Rg3 bile salt-phosphatidylcholine-based mixed micelle system (BS-PC-MMS) was prepared. The optimization of G-Rg3 BS-PC-MMS was carried out using response surface methodology based on a central composite design. The encapsulation efficiency (EE) and light transmission (LT) of the optimized formulation were 90.69±2.54% and 99.10±3.12%, respectively. The average particle size of micelles was 20 nm. To increase the stability of G-Rg3 BS-PC-MMS, the lyophilized formulation of micelles was prepared. The G-Rg3 BS-PC-MMS did not produce hemolysis of erythrocytes within a certain concentration range and exhibited a good inhibition of tumor cells. The chick embryo chorioallantoic membrane assay results showed that the G-Rg3 BS-PC-MMS significantly inhibited angiogenesis. The G-Rg3 BS-PC-MMS is thus shown to be a safe, stable, and promising drug delivery system.
authors.affiliation: College of Chemistry and Chemical Engineering, Liaoning Normal University.
authors.forename: Xiu
authors.initials: X
authors.lastname: Yu
authors.forename: Huan
authors.initials: H
authors.lastname: Xu
authors.forename: Meina
authors.initials: M
authors.lastname: Hu
authors.forename: Xiaojiao
authors.initials: X
authors.lastname: Luan
authors.forename: Kaiqian
authors.initials: K
authors.lastname: Wang
authors.forename: Yuanshan
authors.initials: Y
authors.lastname: Fu
authors.forename: Di
authors.initials: D
authors.lastname: Zhang
authors.forename: Jianying
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D001647:Bile Acids and Salts / Q000737:chemistry* / Q000494:pharmacology; D045744:Cell Line, Tumor; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000098:blood supply / Q000187:drug effects; D036145:Ginsenosides / Q000737:chemistry* / Q000494:pharmacology; D006461:Hemolysis; D006801:Humans; D008823:Micelles; D018919:Neovascularization, Physiologic / Q000187:drug effects; D010713:Phosphatidylcholines / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D001647:Bile Acids and Salts; D036145:Ginsenosides; D008823:Micelles; D010713:Phosphatidylcholines; C097367:ginsenoside Rg3