title: Muscle stem cells contribute to myofibres in sedentary adult mice.
abstract: Skeletal muscle is essential for mobility, stability and whole body metabolism, and muscle loss, for instance, during sarcopenia, has profound consequences. Satellite cells (muscle stem cells) have been hypothesized, but not yet demonstrated, to contribute to muscle homeostasis and a decline in their contribution to myofibre homeostasis to play a part in sarcopenia. To test their role in muscle maintenance, we genetically labelled and ablated satellite cells in adult sedentary mice. We demonstrate via genetic lineage experiments that, even in the absence of injury, satellite cells contribute to myofibres in all adult muscles, although the extent and timing differs. However, genetic ablation experiments showed that satellite cells are not globally required to maintain myofibre cross-sectional area of uninjured adult muscle.
authors.affiliation: Department of Human Genetics, University of Utah, Salt Lake City, Utah 84112 USA.
authors.forename: Alexandra C
authors.initials: AC
authors.lastname: Keefe
authors.affiliation: Department of Human Genetics, University of Utah, Salt Lake City, Utah 84112 USA.
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: Lawson
authors.affiliation: Department of Human Genetics, University of Utah, Salt Lake City, Utah 84112 USA.
authors.forename: Steven D
authors.initials: SD
authors.lastname: Flygare
authors.affiliation: Department of Human Genetics, University of Utah, Salt Lake City, Utah 84112 USA.
authors.forename: Zachary D
authors.initials: ZD
authors.lastname: Fox
authors.affiliation: Department of Human Genetics, University of Utah, Salt Lake City, Utah 84112 USA.
authors.forename: Mary P
authors.initials: MP
authors.lastname: Colasanto
authors.affiliation: Department of Human Genetics, University of Utah, Salt Lake City, Utah 84112 USA.
authors.forename: Sam J
authors.initials: SJ
authors.lastname: Mathew
authors.affiliation: 1] Department of Human Genetics, University of Utah, Salt Lake City, Utah 84112 USA [2] USTAR Center for Genetic Discovery, University of Utah, Salt Lake City, Utah 84112 USA.
authors.forename: Mark
authors.initials: M
authors.lastname: Yandell
authors.affiliation: Department of Human Genetics, University of Utah, Salt Lake City, Utah 84112 USA.
authors.forename: Gabrielle
authors.initials: G
authors.lastname: Kardon
mesh_terms: D000483:Alleles; D000818:Animals; D003433:Crosses, Genetic; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006706:Homeostasis; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018485:Muscle Fibers, Skeletal / Q000473:pathology*; D051763:PAX7 Transcription Factor / Q000378:metabolism; D012038:Regeneration; D055948:Sarcopenia / Q000235:genetics; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology; D013997:Time Factors
chemical_list: D051763:PAX7 Transcription Factor; C497322:Pax7 protein, mouse; D049452:Green Fluorescent Proteins