title: The microRNA-520a-3p inhibits proliferation, apoptosis and metastasis by targeting MAP3K2 in non-small cell lung cancer.
abstract: Growing evidence indicates that miR-520a was involved in the complement attack and migration of tumor cells, but nonetheless, the role of miR-520a-3p in non-small cell lung cancer (NSCLC) is not clear. Mitogen-activated protein kinase kinase kinase 2 (MAP3K2) is a kinase belonging to the serine/threonine protein kinase family. To develop potential therapy targeting MAP3K2, we studied the roles of miR-520a-3p in the proliferation, apoptosis and metastasis of NSCLC. The expression levels of miR-520a-3p were quantified in tumor tissues of NSCLC by qRT-PCR, and the mimics and inhibitors were used to verify the function of miR-520a-3p. The cell proliferation was evaluated by MTT assay, and the migration and invasion was evaluated by transwell assay. The athymic mice subcutaneous injection was used to research NSCLC cell tumor formation. The bioinformatics tools and luciferase assay was applied to detect the relationship between miR-520a-3p and its target. Protein levels of miR-520a-3p target was determined by western blot analysis. MiR-520a-3p expression was decreased in the NSCLC tissues compared with their normal counterparts and lower expression of miR-520a-3p in NSCLC tissues was associated with a higher clinical stage, NSCLC metastasis and poor prognosis. Inhibition of expression of miR-520a-3p can reduce in vitro NSCLC cell migration and invasion as well as in vivo metastasis. MAP3K2 mRNA contains a binding site for miR-520a-3p in the 3'UTR. MAP3K2 is one of target of miR-520a-3p. Together, our data demonstrated that miR-520a-3p inhibits proliferation, apoptosis and metastasis in NSCLC by targeting MAP3K2, and miR-520a-3p may be used as a prognosis marker for NSCLC in clinical research.
authors.affiliation: Department of Thoracic Surgery, Institute of Surgery Research, Daping Hospital, The Third Military Medical University Chongqing 400038, P. R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Thoracic Surgery, Institute of Surgery Research, Daping Hospital, The Third Military Medical University Chongqing 400038, P. R. China.
authors.forename: Qunyou
authors.initials: Q
authors.lastname: Tan
authors.affiliation: Department of Thoracic Surgery, Institute of Surgery Research, Daping Hospital, The Third Military Medical University Chongqing 400038, P. R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Deng
authors.affiliation: Department of Thoracic Surgery, Institute of Surgery Research, Daping Hospital, The Third Military Medical University Chongqing 400038, P. R. China.
authors.forename: Chunshu
authors.initials: C
authors.lastname: Fang
authors.affiliation: Department of Thoracic Surgery, Institute of Surgery Research, Daping Hospital, The Third Military Medical University Chongqing 400038, P. R. China.
authors.forename: Di
authors.initials: D
authors.lastname: Qi
authors.affiliation: Department of Thoracic Surgery, Institute of Surgery Research, Daping Hospital, The Third Military Medical University Chongqing 400038, P. R. China.
authors.forename: Ruwen
authors.initials: R
authors.lastname: Wang
keywords: MAP3K2; MiR-520a-3p; metastasis; non-small cell lung cancer; prognosis