title: Human Papillomavirus Vaccine-Induced Cytokine Messenger RNA Expression in Vaccinated Women.
abstract: The objective of this work was to evaluate the influence of human papillomavirus (HPV) vaccination on peripheral blood mononuclear cell (PBMC) proliferation and cytokine gene transcription. PBMCs isolated after HPV immunization were incubated with HPV vaccine, phytohemagglutinin, or buffer. Cell proliferation was assessed by MTT reduction assay. RNA was extracted from PBMCs, and the relative concentration of cytokine messenger RNA (mRNA) transcripts (IFN-β, IFN-γ, IL-12, TNF-α, IL-6, IL-17, or IL-10) relative to transcription of the β-actin gene was determined by real-time polymerase chain reaction. PBMC proliferation in response to HPV vaccine and PHA were greater than that observed in unstimulated cells (p<0.001). Cytokine mRNAs were upregulated in stimulated PBMC cultures. The median increase in vaccine-stimulated cultures was: IFN-β=334.4-fold; IL-12=46.33-fold; IFN-γ=12.64-fold; IL-6=9.07-fold; IL-17=7.33-fold; IL-10=6.47-fold; and TNF-α=2.36-fold. The IFN-β expression was significantly higher (p<0.05). Proliferative PBMC responses and multiple cytokine gene expression were detected in women who received the HPV vaccine.
authors.affiliation: 1 Department of Gynecology and Obstetrics, Federal University of Rio Grande do Norte , Natal, Brazil .
authors.forename: Ana Katherine
authors.initials: AK
authors.lastname: Gonçalves
authors.affiliation: 2 Department of Gynecology and Obstetrics, State University of Campinas , Campinas, Brazil .
authors.forename: Paulo César
authors.initials: PC
authors.lastname: Giraldo
authors.affiliation: 3 Department of Clinical Analysis and Toxicology, Federal University of Rio Grande do Norte , Natal, Brazil .
authors.forename: Paula Renata Lima
authors.initials: PR
authors.lastname: Machado
authors.affiliation: 4 Department of Microbiology and Parasitology, Federal University of Rio Grande do Norte , Natal, Brazil .
authors.forename: Kleber Juvenal Silva
authors.initials: KJ
authors.lastname: Farias
authors.affiliation: 3 Department of Clinical Analysis and Toxicology, Federal University of Rio Grande do Norte , Natal, Brazil .
authors.forename: Ana Paula Ferreira
authors.initials: AP
authors.lastname: Costa
authors.affiliation: 3 Department of Clinical Analysis and Toxicology, Federal University of Rio Grande do Norte , Natal, Brazil .
authors.forename: Janaina Cristiana de Oliveira Crispim
authors.initials: JC
authors.lastname: Freitas
authors.affiliation: 5 Department of Gynecology and Obstetrics, Federal University of Ceará , Fortaleza, Brazil .
authors.forename: José
authors.initials: J
authors.lastname: Eleutério
authors.affiliation: 6 Department of Obstetrics and Gynecology, Weill Cornell Medical College , New York.
authors.forename: Steven S
authors.initials: SS
authors.lastname: Witkin
mesh_terms: D049109:Cell Proliferation; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D010084:Oxidation-Reduction; D027383:Papillomaviridae; D053918:Papillomavirus Vaccines / Q000008:administration & dosage / Q000276:immunology*; D012333:RNA, Messenger / Q000032:analysis*; D060888:Real-Time Polymerase Chain Reaction; D013778:Tetrazolium Salts / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism
chemical_list: D016207:Cytokines; D053918:Papillomavirus Vaccines; D012333:RNA, Messenger; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue