title: Both FOXO3a and FOXO1 are involved in the HGF-protective pathway against apoptosis in endothelial cells.
abstract: Hepatocyte growth factor (HGF) was identified as an endogenous tissue protective agent against apoptosis in many cell types. The mechanism by which HGF protects primary endothelial cells (ECs) has not yet been completely elucidated. FOXO1 and FOXO3a, two members of the FOXO family, are the most abundant FOXO isoforms in mature endothelial cells. In this study, we aimed to explore whether FOXO1 and FOXO3a play similar roles in HGF-mediated protection against apoptosis in mature endothelial cells. Our result showed that HGF prevented ECs from oxidative-stress induced apoptosis in part by inducing the phosphorylation of FOXO proteins. FOXO1 and FOXO3a are equally important in this process by regulating the expression of Bim, PUMA, FasL, and TRAIL.
authors.affiliation: Biomedical Research Institute, Shenzhen Peking University-the Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Cardiovascularology, Peking University Shenzhen Hospital, Shenzhen, Guangdong, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Qu
authors.affiliation: Department of Emergency Surgery, Peking University Shenzhen Hospital, Shenzhen, Guangdong, China.
authors.forename: Heng-Chang
authors.initials: HC
authors.lastname: Cao
authors.affiliation: Department of Cardiovascularology, Peking University Shenzhen Hospital, Shenzhen, Guangdong, China.
authors.forename: Mei-Hong
authors.initials: MH
authors.lastname: Li
authors.affiliation: Department of Cardiovascularology, Peking University Shenzhen Hospital, Shenzhen, Guangdong, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.affiliation: Biomedical Research Institute, Shenzhen Peking University-the Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong, China.
authors.forename: Xiao-Fan
authors.initials: XF
authors.lastname: Chen
authors.affiliation: Department of Dermatology, Peking University Shenzhen Hospital, Shenzhen, Guangdong, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yu
authors.affiliation: Department of obstetrics and gynaecology, Peking University Shenzhen Hospital, Shenzhen, Guangdong, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Yu
authors.affiliation: Department of Cardiovascular Sciences, Peking University Health Science Center, Peking University, Peking, China.
authors.forename: Le-Min
authors.initials: LM
authors.lastname: Zheng
authors.affiliation: Biomedical Research Institute, Shenzhen Peking University-the Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong, China.|Department of Dermatology, Peking University Shenzhen Hospital, Shenzhen, Guangdong, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000502:physiology*; D053222:Fas Ligand Protein / Q000235:genetics; D000071161:Forkhead Box Protein O1; D000071316:Forkhead Box Protein O3; D051858:Forkhead Transcription Factors / Q000502:physiology*; D017228:Hepatocyte Growth Factor / Q000502:physiology*; D006801:Humans; D018384:Oxidative Stress / Q000502:physiology; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000235:genetics
chemical_list: C085126:FOXO1 protein, human; C499517:FOXO3 protein, human; D053222:Fas Ligand Protein; D000071161:Forkhead Box Protein O1; D000071316:Forkhead Box Protein O3; D051858:Forkhead Transcription Factors; D053221:TNF-Related Apoptosis-Inducing Ligand; D017228:Hepatocyte Growth Factor
keywords: Akt; Apoptosis; FLIP; FOXO1; FOXO3a; HUVEC