title: Dimensionally stable and bioactive membrane for guided bone regeneration: An in vitro study.
abstract: Composite fibrous electrospun membranes based on poly(dl-lactide) (PLA) and poly(ε-caprolactone) (PCL) were engineered to include borate bioactive glass (BBG) for the potential purposes of guided bone regeneration (GBR). The fibers were characterized using scanning and transmission electron microscopies, which respectively confirmed the submicron fibrous arrangement of the membranes and the successful incorporation of BBG particles. Selected mechanical properties of the membranes were evaluated using the suture pullout test. The addition of BBG at 10 wt % led to similar stiffness, but more importantly, it led to a significantly stronger (2.37 ± 0.51 N mm) membrane when compared with the commercially available Epiguide® (1.06 ± 0.24 N mm) under hydrated conditions. Stability (shrinkage) was determined after incubation in a phosphate buffer solution from 24 h up to 9 days. The dimensional stability of the PLA:PCL-based membranes with or without BBG incorporation (10.07-16.08%) was similar to that of Epiguide (14.28%). Cell proliferation assays demonstrated a higher rate of preosteoblasts proliferation on BBG-containing membranes (6.4-fold) over BBG-free membranes (4- to 5.8-fold) and EpiGuide (4.5-fold), following 7 days of in vitro culture. Collectively, our results demonstrated the ability to synthesize, via electrospinning, stable, polymer-based submicron fibrous BBG-containing membranes capable of sustaining osteoblastic attachment and proliferation-a promising attribute in GBR.
authors.affiliation: Department of Restorative Dentistry, Division of Dental Biomaterials, Indiana University School of Dentistry, Indianapolis, Indiana, 46202.|Department of Periodontics & Allied Programs, Indiana University School of Dentistry, Indianapolis, Indiana, 46202.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Rowe
authors.affiliation: Department of Restorative Dentistry, Division of Dental Biomaterials, Indiana University School of Dentistry, Indianapolis, Indiana, 46202.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Kamocki
authors.affiliation: Department of Restorative Dentistry, Division of Dental Biomaterials, Indiana University School of Dentistry, Indianapolis, Indiana, 46202.
authors.forename: Divya
authors.initials: D
authors.lastname: Pankajakshan
authors.affiliation: Department of Restorative Dentistry, Division of Dental Biomaterials, Indiana University School of Dentistry, Indianapolis, Indiana, 46202.
authors.forename: Ding
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Oral Biology, Indiana University School of Dentistry, Indianapolis, Indiana, 46202.
authors.forename: Angela
authors.initials: A
authors.lastname: Bruzzaniti
authors.affiliation: Department of Materials Science and Engineering, The University of Alabama at Birmingham, Birmingham, Alabama, 35294.
authors.forename: Vinoy
authors.initials: V
authors.lastname: Thomas
authors.affiliation: Department of Periodontics & Allied Programs, Indiana University School of Dentistry, Indianapolis, Indiana, 46202.
authors.forename: Steve B
authors.initials: SB
authors.lastname: Blanchard
authors.affiliation: Department of Restorative Dentistry, Division of Dental Biomaterials, Indiana University School of Dentistry, Indianapolis, Indiana, 46202.
authors.forename: Marco C
authors.initials: MC
authors.lastname: Bottino
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D049109:Cell Proliferation; D008567:Membranes, Artificial; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D011091:Polyesters / Q000737:chemistry*
chemical_list: D008567:Membranes, Artificial; D011091:Polyesters; C016240:polycaprolactone; C033616:poly(lactide)
keywords: bioglass; bone; electrospinning; membranes; regeneration