title: Bone regeneration and gene expression in bone defects under healthy and osteoporotic bone conditions using two commercially available bone graft substitutes.
abstract: Biosilicate(®) and Bio-Oss(®) are two commercially available bone substitutes, however, little is known regarding their efficacy in osteoporotic conditions. The purpose of this study was to evaluate the osteogenic properties of both materials, at tissue and molecular level. Thirty-six Wistar rats were submitted to ovariectomy (OVX) for inducing osteoporotic conditions and sham surgery (SHAM) as a control. Bone defects were created in both femurs, which were filled with Biosilicate(®) or Bio-Oss(®), and empty defects were used as control. For the healthy condition both Biosilicate(®) and Bio-Oss(®) did not improve bone formation after 4 weeks. Histomorphometric evaluation of osteoporotic bone defects with bone substitutes showed more bone formation, significant for Bio-Oss(®). Molecular biological evaluation was performed by gene-expression analysis (Runx-2, ALP, OC, OPG, RANKL). The relative gene expression was increased with Biosilicate(®) for all genes in OVX rats and for Runx-2, ALP, OC and RANKL in SHAM rats. In contrast, with Bio-Oss(®), the relative gene expression of OVX rats was similar for all three groups. For SHAM rats it was increased for Runx-2, ALP, OC and RANKL. Since both materials improved bone regeneration in osteoporotic conditions, our results suggest that bone defects in osteoporotic conditions can be efficiently treated with these two bone substitutes.
authors.affiliation: Department of Biomaterials, Radboudumc, 6500 HB Nijmegen, The Netherlands.
authors.forename: Claire I A
authors.initials: CI
authors.lastname: van Houdt
authors.forename: Carla R
authors.initials: CR
authors.lastname: Tim
authors.forename: Murilo C
authors.initials: MC
authors.lastname: Crovace
authors.forename: Edgar D
authors.initials: ED
authors.lastname: Zanotto
authors.forename: Oscar
authors.initials: O
authors.lastname: Peitl
authors.forename: Dietmar J O
authors.initials: DJ
authors.lastname: Ulrich
authors.forename: John A
authors.initials: JA
authors.lastname: Jansen
authors.forename: Nivaldo A
authors.initials: NA
authors.lastname: Parizotto
authors.forename: Ana C
authors.initials: AC
authors.lastname: Renno
authors.forename: Jeroen J J P
authors.initials: JJ
authors.lastname: van den Beucken
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics; D000818:Animals; D001861:Bone Regeneration / Q000235:genetics / Q000502:physiology; D018786:Bone Substitutes / Q000737:chemistry; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics; D004195:Disease Models, Animal; D005260:Female; D015870:Gene Expression; D005898:Glass / Q000737:chemistry; D008422:Materials Testing; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology; D008903:Minerals / Q000737:chemistry; D015675:Osteocalcin / Q000235:genetics; D010024:Osteoporosis / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D053244:Osteoprotegerin / Q000235:genetics; D053245:RANK Ligand / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: C077540:Bio-Oss; D018786:Bone Substitutes; D050659:Core Binding Factor Alpha 1 Subunit; D008903:Minerals; D053244:Osteoprotegerin; D053245:RANK Ligand; C493717:Runx2 protein, rat; C506032:Tnfrsf11b protein, rat; C120461:sodium oxide-calcium oxide-silicon dioxide-diphosphorus pentoxide; D015675:Osteocalcin; D000469:Alkaline Phosphatase