title: Differential reactivity of salivary igA and igG against streptococcus mutans proteins in humans with different caries experience.
abstract: Dental caries is an infectious disease which still constitutes a public health concern. It begins at an early age and is caused mainly Streptococcus mutans (S. mutans). The aim of this study was to characterize the salivary humor immune response to S. mutans proteins in patients with caries, with history of caries and without caries, in order to determine which S. mutans proteins participate in the immunological response in subjects with different caries experience. Saliva was collected by spontaneous salivation for 5 minutes from 60 subjects aged 18 to 30 years, classified according to their caries experience as: without caries (Group I), with active caries (Group II) and with history of caries (Group III). The antigens derived from S. mutans by sonication were recognized by salivary IgA and IgG by Western Blot. The results showed that all the individuals studied recognized S. mutans proteins with molecular weights in the range of 8 to 191 kDa, with similar recognition profiles for salivary IgA and IgG. Subjects without caries recognized the 29 kDa protein, also known as S. mutans Antigen A, via salivary IgA, differing from patients with caries and history of caries, who recognized it via IgG. The protective response against S. mutans is mediated by IgA. To conclude, a differential response to the 29 kDa protein between study individuals may be indicative of resistance to dental caries and may have a protective role in the induction of IgA antibodies against dental caries, as found in the group without caries, in contrast to subjects with active caries and history of caries.
authors.affiliation: Center for Dental Research, School of Dentistry, Pontificia Universidad Javeriana, Bogotá, Colombia.
authors.forename: Soledad I
authors.initials: SI
authors.lastname: Gómez
authors.affiliation: Center for Dental Research, School of Dentistry, Pontificia Universidad Javeriana, Bogotá, Colombia.
authors.forename: Lorenza M
authors.initials: LM
authors.lastname: Jaramillo
authors.affiliation: Center for Dental Research, School of Dentistry, Pontificia Universidad Javeriana, Bogotá, Colombia.
authors.forename: Gloria C
authors.initials: GC
authors.lastname: Moreno
authors.affiliation: Center for Dental Research, School of Dentistry, Pontificia Universidad Javeriana, Bogotá, Colombia.
authors.forename: Nelly S
authors.initials: NS
authors.lastname: Roa
authors.affiliation: Center for Dental Research, School of Dentistry, Pontificia Universidad Javeriana, Bogotá, Colombia.
authors.forename: Adriana
authors.initials: A
authors.lastname: Rodríguez
mesh_terms: D000907:Antibodies, Bacterial; D003731:Dental Caries; D006801:Humans; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D012463:Saliva; D013295:Streptococcus mutans
chemical_list: D000907:Antibodies, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G