title: Single Cell Wall Nonlinear Mechanics Revealed by a Multiscale Analysis of AFM Force-Indentation Curves.
abstract: Individual plant cells are rather complex mechanical objects. Despite the fact that their wall mechanical strength may be weakened by comparison with their original tissue template, they nevertheless retain some generic properties of the mother tissue, namely the viscoelasticity and the shape of their walls, which are driven by their internal hydrostatic turgor pressure. This viscoelastic behavior, which affects the power-law response of these cells when indented by an atomic force cantilever with a pyramidal tip, is also very sensitive to the culture media. To our knowledge, we develop here an original analyzing method, based on a multiscale decomposition of force-indentation curves, that reveals and quantifies for the first time the nonlinearity of the mechanical response of living single plant cells upon mechanical deformation. Further comparing the nonlinear strain responses of these isolated cells in three different media, we reveal an alteration of their linear bending elastic regime in both hyper- and hypotonic conditions.
authors.affiliation: Centre National de la Recherche Scientifique UMR5672, Laboratoire de Physique, Ecole Normale Supérieure de Lyon, Université de Lyon I, France.
authors.forename: Simona
authors.initials: S
authors.lastname: Digiuni
authors.affiliation: Centre National de la Recherche Scientifique UMR5667, Laboratoire de Reproduction et de Développement des Plantes, Ecole Normale Supérieure de Lyon, Université de Lyon I, France.
authors.forename: Annik
authors.initials: A
authors.lastname: Berne-Dedieu
authors.affiliation: Centre National de la Recherche Scientifique UMR5672, Laboratoire de Physique, Ecole Normale Supérieure de Lyon, Université de Lyon I, France.
authors.forename: Cristina
authors.initials: C
authors.lastname: Martinez-Torres
authors.affiliation: Centre National de la Recherche Scientifique UMR5667, Laboratoire de Reproduction et de Développement des Plantes, Ecole Normale Supérieure de Lyon, Université de Lyon I, France.
authors.forename: Judit
authors.initials: J
authors.lastname: Szecsi
authors.affiliation: Centre National de la Recherche Scientifique UMR5667, Laboratoire de Reproduction et de Développement des Plantes, Ecole Normale Supérieure de Lyon, Université de Lyon I, France.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Bendahmane
authors.affiliation: Centre National de la Recherche Scientifique UMR5672, Laboratoire de Physique, Ecole Normale Supérieure de Lyon, Université de Lyon I, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Arneodo
authors.affiliation: Centre National de la Recherche Scientifique UMR5672, Laboratoire de Physique, Ecole Normale Supérieure de Lyon, Université de Lyon I, France. Electronic address: fargoul@ens-lyon.fr.
authors.forename: Françoise
authors.initials: F
authors.lastname: Argoul
mesh_terms: D017360:Arabidopsis / Q000166:cytology; D001696:Biomechanical Phenomena; D002473:Cell Wall / Q000737:chemistry*; D004548:Elasticity; D018625:Microscopy, Atomic Force; D014783:Viscosity