title: GPRC6a is not required for the effects of a high-protein diet on body weight in mice.
abstract: OBJECTIVE
  The G-protein coupled receptor family C group 6 member A (GPRC6A) is activated by proteinogenic amino acids and may sense amino acids in the gastrointestinal tract and the brain. The study investigated whether GPRC6A was necessary for the effects of low- and high-protein diets on body weight and food intake in mice.
  
  
  METHODS
  The role of GPRC6A in mediating the effects of a low-protein diet on body weight was investigated in GPRC6a knockout (GPRC6a-KO) and wild-type (WT) mice fed a control diet (18% protein) or a low-protein diet (6% protein) for 9 days. The role of GPRC6A in mediating the effects of a high-protein diet on body weight was investigated in GPRC6a-KO and WT mice fed a control diet (18% protein) or a high-protein diet (50% protein) for 5 weeks.
  
  
  RESULTS
  A high-protein diet reduced body weight gain and food intake compared with a control diet in both WT and GPRC6a-KO mice. A low-protein diet decreased body weight gain in GPRC6a-KO mice.
  
  
  CONCLUSIONS
  GPRC6A was not necessary for the effects of a low- or high-protein diet on body weight and likely does not play a role in protein-induced satiety.
authors.affiliation: Section of Investigative Medicine, Department of Medicine, Imperial College London, Hammersmith Hospital, London, UK.
authors.forename: James S
authors.initials: JS
authors.lastname: Kinsey-Jones
authors.affiliation: Section of Investigative Medicine, Department of Medicine, Imperial College London, Hammersmith Hospital, London, UK.
authors.forename: Amin
authors.initials: A
authors.lastname: Alamshah
authors.affiliation: Section of Investigative Medicine, Department of Medicine, Imperial College London, Hammersmith Hospital, London, UK.
authors.forename: Anne K
authors.initials: AK
authors.lastname: McGavigan
authors.affiliation: Section of Investigative Medicine, Department of Medicine, Imperial College London, Hammersmith Hospital, London, UK.
authors.forename: Eleanor
authors.initials: E
authors.lastname: Spreckley
authors.affiliation: Section of Investigative Medicine, Department of Medicine, Imperial College London, Hammersmith Hospital, London, UK.
authors.forename: Katherine
authors.initials: K
authors.lastname: Banks
authors.affiliation: Section of Investigative Medicine, Department of Medicine, Imperial College London, Hammersmith Hospital, London, UK.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Cereceda Monteoliva
authors.affiliation: Section of Investigative Medicine, Department of Medicine, Imperial College London, Hammersmith Hospital, London, UK.
authors.forename: Mariana
authors.initials: M
authors.lastname: Norton
authors.affiliation: Section of Investigative Medicine, Department of Medicine, Imperial College London, Hammersmith Hospital, London, UK.|Division of Diabetes and Nutritional Sciences, King's College London, Guy's Campus, London, UK.
authors.forename: Gavin A
authors.initials: GA
authors.lastname: Bewick
authors.affiliation: Section of Investigative Medicine, Department of Medicine, Imperial College London, Hammersmith Hospital, London, UK.
authors.forename: Kevin G
authors.initials: KG
authors.lastname: Murphy
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects*; D004044:Dietary Proteins / Q000008:administration & dosage*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D015430:Weight Gain / Q000187:drug effects
chemical_list: D004044:Dietary Proteins; C488584:GPRC6A protein, human; D043562:Receptors, G-Protein-Coupled