title: Induction of Defense-Related Enzymes in Banana Plants: Effect of Live and Dead Pathogenic Strain of Fusarium oxysporum f. sp. cubense.
abstract: The aim of the present study was to scrutinize the response of banana (Grand Naine variety) plants when interacting with dead or live pathogen, Fusarium oxysporum f.sp. cubense, a causative agent of Panama disease. Response of plants was evaluated in terms of induction of defense-related marker enzyme activity, namely, peroxidase (POX), polyphenol oxidase (PPO), β-1,3 glucanase, chitinase, and phenolics. Plant's interaction with live pathogen resulted in early induction of defense to restrain penetration as well as antimicrobial productions. However, pathogen overcame the defense of plant and caused disease. Interaction with dead pathogen resulted in escalating defense response in plants. Later on plants inoculated with dead pathogen showed resistance to even forced inoculation of live pathogen. Results obtained in the present study suggest that dead pathogen was able to mount defense response in plants and provide resistance to Panama disease upon subsequent exposure. Therefore, preparation from dead pathogen could be a potential candidate as a biocontrol agent or plant vaccine to combat Panama disease.
authors.affiliation: Department of Biotechnology, PD Patel Institute of Applied Sciences, Charotar University of Science and Technology, CARUSAT Campus, Gujarat, Changa 388421, India.
authors.forename: Janki N
authors.initials: JN
authors.lastname: Thakker
authors.affiliation: Department of Biotechnology, PD Patel Institute of Applied Sciences, Charotar University of Science and Technology, CARUSAT Campus, Gujarat, Changa 388421, India.
authors.forename: Samiksha
authors.initials: S
authors.lastname: Patel
authors.affiliation: Department of Integrated Biotechnology, Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied Sciences, Gujarat, New Vallabh Vidyanagar 388 121, India.
authors.forename: Pinakin C
authors.initials: PC
authors.lastname: Dhandhukia