title: Antioxidant activity of polysaccharide purified from Acanthopanax koreanum Nakai stems in vitro and in vivo zebrafish model.
abstract: In this study, the antioxidative effects of a purified polysaccharide isolated from the stems of Acanthopanax koreanum Nakai (ASP) on hydrogen peroxide-induced oxidative stress was investigated both in vitro and in vivo using a zebrafish model. A. koreanum Nakai stem was hydrolyzed using five carbohydrases and five proteases for the enzyme-assistant extraction. Of the enzyme-assistant extracts, the Protamex extract exhibited the highest yield and a potent scavenging activity against free radicals. Ethanol-added separation and anion exchange chromatography were conducted to identify the active polysaccharide. The purified polysaccharide significantly scavenged hydrogen peroxide and reduced hydrogen peroxide-induced cell death in Vero cells and in zebrafish. The results reveal that ASP is a useful antioxidant polysaccharide and might be available for relevant industrial applications.
authors.affiliation: Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Min-Cheol
authors.initials: MC
authors.lastname: Kang
authors.affiliation: Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Seo-Young
authors.initials: SY
authors.lastname: Kim
authors.affiliation: Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Eun-A
authors.initials: EA
authors.lastname: Kim
authors.affiliation: Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Ji-Hyeok
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Jeju Aloe Agriculture Association Cooperation, Sehwa-ri, Pyosun-myeon, Seogwipo, Jeju-do 690-1, Republic of Korea.
authors.forename: Young-Sun
authors.initials: YS
authors.lastname: Kim
authors.affiliation: Jeju Aloe Agriculture Association Cooperation, Sehwa-ri, Pyosun-myeon, Seogwipo, Jeju-do 690-1, Republic of Korea.
authors.forename: Seok-Kyu
authors.initials: SK
authors.lastname: Yu
authors.affiliation: Cosmetic Research Center, Coway Co. Ltd., 459-11 Gasan-dong, Geumcheon-gu, Seoul, Republic of Korea.
authors.forename: Jun Byoung
authors.initials: JB
authors.lastname: Chae
authors.affiliation: University of Science and Technology, Daejeon 305-350, Republic of Korea.
authors.forename: In-Hu
authors.initials: IH
authors.lastname: Choe
authors.affiliation: Cosmetic Research Center, Coway Co. Ltd., 459-11 Gasan-dong, Geumcheon-gu, Seoul, Republic of Korea.
authors.forename: Jin Hun
authors.initials: JH
authors.lastname: Cho
authors.affiliation: Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea. Electronic address: youjinj@jejunu.ac.kr.
authors.forename: You-Jin
authors.initials: YJ
authors.lastname: Jeon
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D027481:Araliaceae / Q000737:chemistry*; D016923:Cell Death / Q000187:drug effects; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D005609:Free Radicals / Q000037:antagonists & inhibitors; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors / Q000494:pharmacology; D023421:Models, Animal; D018384:Oxidative Stress / Q000187:drug effects; D018547:Plant Stems / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D014709:Vero Cells; D015027:Zebrafish
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D011134:Polysaccharides; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide
keywords: Acanthopanax koreanum Nakai; Antioxidant activity; Enzyme-assistant extract; Oxidative stress; Polysaccharide; Zebrafish