title: The Functional Variant in the 3'UTR of PTPRT with the Risk of Esophageal Squamous Cell Carcinoma in a Chinese Population.
abstract: OBJECTIVE
  PTPRT is an essential tumor suppressor that plays crucial roles in regulating the mechanisms of tumorigenesis. Polymorphisms in PTPRT have been reported associated with human longevity, but their association with the risk of esophageal squamous cell carcinoma (ESCC) has not been found so far. In this study, we focused on the miRNAs associated SNPs in the 3'-UTR of PTPRT to investigate the further relationship of the SNPs with miRNAs among Chinese ESCC patients.
  
  
  METHODS
  We performed case-control study including 790 ESCC patients and 749 cancer-free controls. Genotyping, real time PCR assay, cell transfection and the dual luciferase reporter assay were used in our study.
  
  
  RESULTS
  We found that patients suffering from smoking exposure, drinking exposure and the history of cancer indicated to be the susceptible population by comparing with controls. Besides, SNP rs2866943 in PTPRT 3'-UTR was involved in the occurrence of ESCC by acting as a protective factor while rs6029959 acting a risk factor. SNP rs2866943 was also could be regulated by miR-218 which caused a down-regulation of PTPRT in patients with CT and TT genotype. Furthermore, the carriers of CT and TT genotype presented a small tumor size as well as the low probability of metastasis.
  
  
  CONCLUSIONS
  Our findings have shown that the SNP rs2866943 in PTPRT 3'-UTR, through disrupting the regulatory role of miR-218 in PTPRT expression, rs2866943 in PTPRT might act as a protective factor in the pathogenesis of ESCC.
authors.affiliation: Department of clinical laboratory, The First People's Hospital of Kunshan, Affiliated Hospital of Jiangsu University, Affiliated to Jiangsu University, Kunshan, Jiangsu, China.
authors.forename: Yongliang
authors.initials: Y
authors.lastname: Yao
authors.forename: Jie
authors.initials: J
authors.lastname: Shao
authors.forename: Jianhong
authors.initials: J
authors.lastname: Wu
authors.forename: Qinghui
authors.initials: Q
authors.lastname: Zhang
authors.forename: Jianjun
authors.initials: J
authors.lastname: Wang
authors.forename: Daping
authors.initials: D
authors.lastname: Xiao
authors.forename: Feng
authors.initials: F
authors.lastname: Huang
mesh_terms: D020413:3' Untranslated Regions; D000368:Aged; D044466:Asian People / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000235:genetics*; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D002681:China; D004938:Esophageal Neoplasms / Q000097:blood / Q000235:genetics*; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D054623:Receptor-Like Protein Tyrosine Phosphatases, Class 2 / Q000235:genetics*; D012307:Risk Factors
chemical_list: D020413:3' Untranslated Regions; C545189:MIRN128 microRNA, human; D035683:MicroRNAs; C516865:PTPRT protein, human; D054623:Receptor-Like Protein Tyrosine Phosphatases, Class 2