title: Fish Myogenic Regulatory Protein LUC7L: Characterization and Expression Analysis in Korean Rose Bitterling (Rhodeus uyekii).
abstract: Serine-arginine-rich nuclear protein LUC7L plays an important role in the regulation of myogenesis in mice. In the present study, we isolated and characterized the Korean rose bitterling Rhodeus uyekii Luc7l cDNA, designated RuLuc7l. The RuLuc7l cDNA is 1,688 bp long and encodes a 364-amino-acid polypeptide containing serine/arginine-rich region at the C-terminus. The deduced RuLuc7l protein has high amino acid identity (71-97%) with those of other species including human. Phylogenetic analysis revealed that RuLUC7L clustered with fish LUC7L proteins. The expression of RuLuc7l mRNA was high in the brain, kidney, and stomach of Korean rose bitterling. Expression of the RuLuc7l mRNA was detected from 1 day post-fertilization (dpf) and moderately increased until 21 dpf during the early development. Further investigations are required to elucidate the functional role of RuLUC7L in myogenesis in R. uyekii.
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute, Busan 619-705, Korea.
authors.forename: Ju Lan
authors.initials: JL
authors.lastname: Kim
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute, Busan 619-705, Korea.
authors.forename: Hee Jeong
authors.initials: HJ
authors.lastname: Kong
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute, Busan 619-705, Korea.
authors.forename: Hyung Soo
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute, Busan 619-705, Korea.
authors.forename: Woo-Jin
authors.initials: WJ
authors.lastname: Kim
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute, Busan 619-705, Korea.
authors.forename: Dong-Gyun
authors.initials: DG
authors.lastname: Kim
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute, Busan 619-705, Korea.
authors.forename: Bo-Hye
authors.initials: BH
authors.lastname: Nam
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute, Busan 619-705, Korea.
authors.forename: Young-Ok
authors.initials: YO
authors.lastname: Kim
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute, Busan 619-705, Korea.
authors.forename: Cheul Min
authors.initials: CM
authors.lastname: An
keywords: Development; Korean rose bitterling; Luc7l; Myogenesis; Rhodeus uyekii