title: An Efficient Protocol for Plantlet Regeneration via Direct Organogenesis by Using Nodal Segments from Embryo-Cultured Seedlings of Cinnamomum camphora L.
abstract: A simple and efficient plantlet regeneration protocol via direct organogenesis was established for camphor tree (Cinnamomum camphora L.). Stem segments with one node (SN explants) from embryo-cultured seedlings (EC seedlings) were used as explants. Murashige and Skoog (MS) medium supplemented with 0.5 mg/L 2, 4-dichlorophenoxyacetic acid and 2.0 mg/L 6-benzyladenine was used to induce cotyledonary embryo germination. This medium was also used for EC seedlings propagation and adventitious bud induction from SN explants. Regenerated plantlets were cultured on hormone-free MS medium for elongation and root induction. The regeneration capability of SN explants was compared by using EC seedling lines established in this research. EC seedling line EL6 exhibited the highest adventitious bud induction frequency (91.7%) and the highest number of buds per responding explant (5.2), which was considered as the most efficient EC seedling line for further gene transformation research.
authors.affiliation: School of Life Science and Technology, Nanyang Normal University, Nanyang City, Henan Province, P. R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Du
authors.affiliation: School of Life Science and Technology, Nanyang Normal University, Nanyang City, Henan Province, P. R. China.
authors.forename: Yongpeng
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Life Science and Technology, Nanyang Normal University, Nanyang City, Henan Province, P. R. China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Yao
authors.affiliation: School of Life Science and Technology, Nanyang Normal University, Nanyang City, Henan Province, P. R. China.
authors.forename: Liwei
authors.initials: L
authors.lastname: Zhang
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D001593:Benzyl Compounds; D027423:Cinnamomum camphora / Q000187:drug effects / Q000254:growth & development*; D018548:Cotyledon / Q000187:drug effects / Q000254:growth & development; D003470:Culture Media / Q000737:chemistry; D018525:Germination / Q000187:drug effects / Q000502:physiology; D007701:Kinetin / Q000494:pharmacology; D063246:Organogenesis, Plant / Q000187:drug effects / Q000502:physiology*; D010937:Plant Growth Regulators / Q000494:pharmacology; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development; D018547:Plant Stems / Q000187:drug effects / Q000254:growth & development; D010946:Plants, Medicinal; D011687:Purines; D012038:Regeneration / Q000187:drug effects / Q000502:physiology*; D036226:Seedlings / Q000187:drug effects / Q000254:growth & development*; D012639:Seeds / Q000187:drug effects / Q000254:growth & development*
chemical_list: D001593:Benzyl Compounds; D003470:Culture Media; D010937:Plant Growth Regulators; D011687:Purines; D015084:2,4-Dichlorophenoxyacetic Acid; C480551:benzylaminopurine; D007701:Kinetin