title: Detection of Group A Streptococcus in Pharyngeal Swab Specimens by Use of the AmpliVue GAS Isothermal Helicase-Dependent Amplification Assay.
abstract: We evaluated the clinical performance (sensitivity and specificity) of the AmpliVue group A Streptococcus (GAS) isothermal helicase-dependent amplification assay using 1,192 pharyngeal swab specimens. AmpliVue GAS assay results were compared to the results of routine throat cultures on selective streptococcal blood agar plates. The sensitivity and specificity of the AmpliVue GAS assay were 98.3% (95% confidence interval [CI], 95 to 100%) and 93.2% (95% CI, 91 to 95%), respectively.
authors.affiliation: Medical College of Wisconsin, Milwaukee, Wisconsin, USA.
authors.forename: Matthew L
authors.initials: ML
authors.lastname: Faron
authors.affiliation: Medical College of Wisconsin, Milwaukee, Wisconsin, USA.
authors.forename: Nathan A
authors.initials: NA
authors.lastname: Ledeboer
authors.affiliation: Laboratory Alliance of Central New York, Syracuse, New York, USA.
authors.forename: Paul
authors.initials: P
authors.lastname: Granato
authors.affiliation: University of Utah, Salt Lake City, Utah, USA Primary Children's Medical Center, Salt Lake City, Utah, USA.
authors.forename: Judy A
authors.initials: JA
authors.lastname: Daly
authors.affiliation: University of Utah, Salt Lake City, Utah, USA.
authors.forename: Kristina
authors.initials: K
authors.lastname: Pierce
authors.affiliation: The Ohio State University Medical Center, Columbus, Ohio, USA.
authors.forename: Preeti
authors.initials: P
authors.lastname: Pancholi
authors.affiliation: Marshfield Labs, Marshfield Clinic, Marshfield, Wisconsin, USA.
authors.forename: Timothy S
authors.initials: TS
authors.lastname: Uphoff
authors.affiliation: Medical College of Wisconsin, Milwaukee, Wisconsin, USA bbuchan@mcw.edu.
authors.forename: Blake W
authors.identifier: http://orcid.org/0000-0002-4111-048X
authors.initials: BW
authors.lastname: Buchan
mesh_terms: D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D010614:Pharynx / Q000382:microbiology*; D012680:Sensitivity and Specificity; D013290:Streptococcal Infections / Q000175:diagnosis*; D013297:Streptococcus pyogenes / Q000235:genetics / Q000302:isolation & purification*