title: ATG14 controls SNARE-mediated autophagosome fusion with a lysosome.
abstract: Autophagosome fusion with a lysosome constitutes the last barrier for autophagic degradation. It is speculated that this fusion process is precisely and tightly regulated. Recent genetic evidence suggests that a set of SNARE proteins, including STX17, SNAP29, and VAMP8, are essential for the fusion between autophagosomes and lysosomes. However, it remains unclear whether these SNAREs are fusion competent and how their fusogenic activity is specifically regulated during autophagy. Using a combination of biochemical, cell biology, and genetic approaches, we demonstrated that fusogenic activity of the autophagic SNARE complex is temporally and spatially controlled by ATG14/Barkor/Atg14L, an essential autophagy-specific regulator of the class III phosphatidylinositol 3-kinase complex (PtdIns3K). ATG14 directly binds to the STX17-SNAP29 binary complex on autophagosomes and promotes STX17-SNAP29-VAMP8-mediated autophagosome fusion with lysosomes. ATG14 homo-oligomerization is required for SNARE binding and fusion promotion, but is dispensable for PtdIns3K stimulation and autophagosome biogenesis. Consequently, ATG14 homo-oligomerization is required for autophagosome fusion with a lysosome, but is dispensable for autophagosome biogenesis. These data support a key role of ATG14 in controlling autophagosome fusion with a lysosome.
authors.affiliation: a Center for Autophagy Research; Department of Internal Medicine; University of Texas Southwestern Medical Center ; Dallas , TX , USA.
authors.forename: Rong
authors.initials: R
authors.lastname: Liu
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Zhi
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhong
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000378:metabolism*; D001343:Autophagy; D006801:Humans; D008247:Lysosomes / Q000378:metabolism*; D008561:Membrane Fusion; D008954:Models, Biological; D010588:Phagosomes / Q000378:metabolism*; D050600:SNARE Proteins / Q000378:metabolism*
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D050600:SNARE Proteins
keywords: ATG14; Barkor; SNARE; autophagosome; homo-oligomerization; lysosome; membrane fusion; membrane tethering; snap29; syntaxin 17; vamp8