title: Microbial community in high arsenic shallow groundwater aquifers in Hetao Basin of Inner Mongolia, China.
abstract: A survey was carried out on the microbial community of 20 groundwater samples (4 low and 16 high arsenic groundwater) and 19 sediments from three boreholes (two high arsenic and one low arsenic boreholes) in a high arsenic groundwater system located in Hetao Basin, Inner Mongolia, using the 454 pyrosequencing approach. A total of 233,704 sequence reads were obtained and classified into 12-267 operational taxonomic units (OTUs). Groundwater and sediment samples were divided into low and high arsenic groups based on measured geochemical parameters and microbial communities, by hierarchical clustering and principal coordinates analysis. Richness and diversity of the microbial communities in high arsenic sediments are higher than those in high arsenic groundwater. Microbial community structure was significantly different either between low and high arsenic samples or between groundwater and sediments. Acinetobacter, Pseudomonas, Psychrobacter and Alishewanella were the top four genera in high arsenic groundwater, while Thiobacillus, Pseudomonas, Hydrogenophaga, Enterobacteriaceae, Sulfuricurvum and Arthrobacter dominated high arsenic sediments. Archaeal sequences in high arsenic groundwater were mostly related to methanogens. Biota-environment matching and co-inertia analyses showed that arsenic, total organic carbon, SO4(2-), SO4(2-)/total sulfur ratio, and Fe(2+) were important environmental factors shaping the observed microbial communities. The results of this study expand our current understanding of microbial ecology in high arsenic groundwater aquifers and emphasize the potential importance of microbes in arsenic transformation in the Hetao Basin, Inner Mongolia.
authors.affiliation: State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, 430074, PRC.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.affiliation: State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, 430074, PRC; School of Environmental Studies, China University of Geosciences, Wuhan, 430074, PRC.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, 430074, PRC.
authors.forename: Xinyue
authors.initials: X
authors.lastname: Dai
authors.affiliation: State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, 430074, PRC.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, 430074, PRC; School of Environmental Studies, China University of Geosciences, Wuhan, 430074, PRC.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, 430074, PRC.
authors.forename: Dawei
authors.initials: D
authors.lastname: Jiang
authors.affiliation: Institute of Earth Sciences, China University of Geosciences, Beijing, 10083, China.
authors.forename: Shang
authors.initials: S
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, 430074, PRC.
authors.forename: Hongchen
authors.initials: H
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, 430074, PRC; School of Environmental Studies, China University of Geosciences, Wuhan, 430074, PRC.
authors.forename: Yanxin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, 430074, PRC; Department of Geology and Environmental Earth Science, Miami University, Oxford, OH, 45056, United States of America.
authors.forename: Hailiang
authors.initials: H
authors.lastname: Dong
mesh_terms: D000150:Acinetobacter / Q000235:genetics / Q000302:isolation & purification; D001151:Arsenic / Q000737:chemistry*; D001173:Arthrobacter / Q000235:genetics / Q000302:isolation & purification; D002681:China; D042621:Comamonadaceae / Q000235:genetics / Q000302:isolation & purification; D004755:Enterobacteriaceae / Q000235:genetics / Q000302:isolation & purification; D060587:Groundwater / Q000737:chemistry / Q000382:microbiology*; D064307:Microbiota; D058889:Molecular Typing; D011549:Pseudomonas; D044096:Psychrobacter / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D020592:Shewanella / Q000235:genetics / Q000302:isolation & purification; D012988:Soil Microbiology; D013855:Thiobacillus / Q000235:genetics / Q000302:isolation & purification; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000737:chemistry*
chemical_list: D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical; D001151:Arsenic