title: Isolation and phenotypic characteristics of microparticles in acute respiratory distress syndrome.
abstract: OBJECTIVE
  To investigate the alterations of microparticles in acute respiratory distress syndrome (ARDS) in rats.
  
  
  METHODS
  18 Wistar male rats were randomly divided into three groups: no intervention, sham (saline control) group and ARDS group (LPS induced). Blood was collected from abdominal aorta and microparticles were extracted through multiple rounds of centrifugation. Particles were analyzed by flow cytometry and transmission electron microscope.
  
  
  RESULTS
  The circulating concentration of total microparticles of rats with ARDS induced by lipopolysaccharide (LPS) did not change compared with other two groups. However, ARDS rats expressed higher concentration of leukocyte- and endothelium- derived microparticles in the three groups.
  
  
  CONCLUSIONS
  Our results indicate that leukocyte and endothelial cell-derived particles may play an important role in ARDS. Thus it is important not only to monitor total microparticle levels but also the phenotypes, which may contribute to the prevention and early treatment of ARDS.
authors.affiliation: Department of Respiratory Medicine, South Building P. R. China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Respiratory Medicine, Special Inpatient Unit, Chinese PLA General Hospital Beijing 100853, P. R. China.
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Meng
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Sciences Beijing 100039, P. R. China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Respiratory Medicine, Special Inpatient Unit, Chinese PLA General Hospital Beijing 100853, P. R. China.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Cai
mesh_terms: D000818:Animals; D055252:Cell-Derived Microparticles / Q000473:pathology*; D004195:Disease Models, Animal; D005434:Flow Cytometry; D008297:Male; D046529:Microscopy, Electron, Transmission; D010641:Phenotype; D051381:Rats; D017208:Rats, Wistar; D012128:Respiratory Distress Syndrome / Q000097:blood* / Q000473:pathology*
keywords: Acute respiratory distress syndrome; inflammatory mediator; microparticles