title: Assessment of four protocols for rapid bacterial identification from positive blood culture pellets by matrix-assisted laser desorption ionization-time of flight mass spectrometry (Vitek® MS).
abstract: In this study, we developed and compared four protocols to prepare a bacterial pellet from 944 positive blood cultures for direct MALDI-TOF mass spectrometry Vitek® MS analysis. Protocol 4, tested on 200 monomicrobial samples, allowed 83% of bacterial identification. This easy, fast, cheap and accurate method is promising in daily practice, especially to limit broad range antibiotic treatment.
authors.affiliation: Service de Bactériologie-Hygiène hospitalière, Nantes University Hospital, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Thomin
authors.affiliation: Service de Bactériologie-Hygiène hospitalière, Nantes University Hospital, France; EA3826, Thérapeutiques cliniques et expérimentales des infections - Nantes Medicine school, France.
authors.forename: Guillaume Ghislain
authors.initials: GG
authors.lastname: Aubin
authors.affiliation: Service de biostatistique, Le Mans Hospital, France.
authors.forename: Fabrice
authors.initials: F
authors.lastname: Foubert
authors.affiliation: Service de Bactériologie-Hygiène hospitalière, Nantes University Hospital, France; EA3826, Thérapeutiques cliniques et expérimentales des infections - Nantes Medicine school, France. Electronic address: stephane.corvec@chu-nantes.fr.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Corvec
mesh_terms: D001419:Bacteria / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques / Q000191:economics / Q000379:methods*; D001769:Blood / Q000382:microbiology*; D006801:Humans; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000191:economics / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000191:economics / Q000379:methods*
keywords: Blood bottle culture; MALDI-TOF; Rapid identification