title: Global Proteomics Analysis of the Response to Starvation in C. elegans.
abstract: Periodic starvation of animals induces large shifts in metabolism but may also influence many other cellular systems and can lead to adaption to prolonged starvation conditions. To date, there is limited understanding of how starvation affects gene expression, particularly at the protein level. Here, we have used mass-spectrometry-based quantitative proteomics to identify global changes in the Caenorhabditis elegans proteome due to acute starvation of young adult animals. Measuring changes in the abundance of over 5,000 proteins, we show that acute starvation rapidly alters the levels of hundreds of proteins, many involved in central metabolic pathways, highlighting key regulatory responses. Surprisingly, we also detect changes in the abundance of chromatin-associated proteins, including specific linker histones, histone variants, and histone posttranslational modifications associated with the epigenetic control of gene expression. To maximize community access to these data, they are presented in an online searchable database, the Encyclopedia of Proteome Dynamics (http://www.peptracker.com/epd/).
authors.affiliation: From the ‡Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow St, Dundee, United Kingdom, DD15EH.
authors.forename: Mark
authors.initials: M
authors.lastname: Larance
authors.affiliation: From the ‡Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow St, Dundee, United Kingdom, DD15EH.
authors.forename: Ehsan
authors.initials: E
authors.lastname: Pourkarimi
authors.affiliation: From the ‡Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow St, Dundee, United Kingdom, DD15EH.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: From the ‡Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow St, Dundee, United Kingdom, DD15EH.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Brenes Murillo
authors.affiliation: From the ‡Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow St, Dundee, United Kingdom, DD15EH.
authors.forename: Robert
authors.initials: R
authors.lastname: Kent
authors.affiliation: From the ‡Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow St, Dundee, United Kingdom, DD15EH a.i.lamond@dundee.ac.uk a.gartner@dundee.ac.uk.
authors.forename: Angus I
authors.initials: AI
authors.lastname: Lamond
authors.affiliation: From the ‡Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow St, Dundee, United Kingdom, DD15EH a.i.lamond@dundee.ac.uk a.gartner@dundee.ac.uk.
authors.forename: Anton
authors.initials: A
authors.lastname: Gartner
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D033181:Information Dissemination; D020407:Internet; D050356:Lipid Metabolism; D020543:Proteome / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D013312:Stress, Physiological
chemical_list: D029742:Caenorhabditis elegans Proteins; D002843:Chromatin; D006657:Histones; D020543:Proteome