title: Nectin-1 spots as a novel adhesion apparatus that tethers mitral cell lateral dendrites in a dendritic meshwork structure of the developing mouse olfactory bulb.
abstract: Mitral cells project lateral dendrites that contact the lateral and primary dendrites of other mitral cells and granule cell dendrites in the external plexiform layer (EPL) of the olfactory bulb. These dendritic structures are critical for odor information processing, but it remains unknown how they are formed. In immunofluorescence microscopy, the immunofluorescence signal for the cell adhesion molecule nectin-1 was concentrated on mitral cell lateral dendrites in the EPL of the developing mouse olfactory bulb. In electron microscopy, the immunogold particles for nectin-1 were symmetrically localized on the plasma membranes at the contacts between mitral cell lateral dendrites, which showed bilateral darkening without dense cytoskeletal undercoats characteristic of puncta adherentia junctions. We named the contacts where the immunogold particles for nectin-1 were symmetrically accumulated "nectin-1 spots." The nectin-1 spots were 0.21 μm in length on average and the distance between the plasma membranes was 20.8 nm on average. In 3D reconstruction of serial sections, clusters of the nectin-1 spots formed a disc-like structure. In the mitral cell lateral dendrites of nectin-1-knockout mice, the immunogold particles for nectin-1 were undetectable and the plasma membrane darkening was electron-microscopically normalized, but the plasma membranes were partly separated from each other. The nectin-1 spots were further identified between mitral cell lateral and primary dendrites and between mitral cell lateral dendrites and granule cell dendritic spine necks. These results indicate that the nectin-1 spots constitute a novel adhesion apparatus that tethers mitral cell dendrites in a dendritic meshwork structure of the developing mouse olfactory bulb.
authors.affiliation: Division of Molecular and Cellular Biology, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe, Hyogo, 650-0017, Japan.|Division of Pathogenetic Signaling, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe, Hyogo, 650-0047, Japan.|CREST, Japan Science and Technology Agency, Kobe, Japan.
authors.forename: Takahito
authors.initials: T
authors.lastname: Inoue
authors.affiliation: CREST, Japan Science and Technology Agency, Kobe, Japan.|Department of Neural Regeneration and Cell Communication, Mie University Graduate School of Medicine, Tsu, Mie, 514-8507, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Fujiwara
authors.affiliation: Division of Molecular and Cellular Biology, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe, Hyogo, 650-0017, Japan.|CREST, Japan Science and Technology Agency, Kobe, Japan.|Division of Signal Transduction, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe, Hyogo, 650-0017, Japan.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Rikitake
authors.affiliation: Division of Molecular and Cellular Biology, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe, Hyogo, 650-0017, Japan.|Division of Pathogenetic Signaling, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe, Hyogo, 650-0047, Japan.|CREST, Japan Science and Technology Agency, Kobe, Japan.
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Maruo
authors.affiliation: Division of Molecular and Cellular Biology, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe, Hyogo, 650-0017, Japan.|Division of Pathogenetic Signaling, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe, Hyogo, 650-0047, Japan.|CREST, Japan Science and Technology Agency, Kobe, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Mandai
authors.affiliation: Department of Physical Therapy, Faculty of Human Science, Hokkaido Bunkyo University, Eniwa, Hokkaido, 061-1449, Japan.
authors.forename: Kazushi
authors.initials: K
authors.lastname: Kimura
authors.affiliation: Department of Medical Rehabilitation, Faculty of Rehabilitation, Kobe Gakuin University, Kobe, Hyogo, 651-2180, Japan.
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Kayahara
authors.affiliation: CREST, Japan Science and Technology Agency, Kobe, Japan.|Department of Neural Regeneration and Cell Communication, Mie University Graduate School of Medicine, Tsu, Mie, 514-8507, Japan.
authors.forename: Shujie
authors.initials: S
authors.lastname: Wang
authors.affiliation: CREST, Japan Science and Technology Agency, Kobe, Japan.|Department of Neural Regeneration and Cell Communication, Mie University Graduate School of Medicine, Tsu, Mie, 514-8507, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Itoh
authors.affiliation: Department of Neural Regeneration and Cell Communication, Mie University Graduate School of Medicine, Tsu, Mie, 514-8507, Japan.
authors.forename: Kousyoku
authors.initials: K
authors.lastname: Sai
authors.affiliation: CREST, Japan Science and Technology Agency, Kobe, Japan.|Faculty of Health Sciences, Kobe University Graduate School of Health Sciences, Kobe, Hyogo, 654-0142, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Mori
authors.affiliation: Department of Physiology, Graduate School of Medicine, University of Tokyo, Tokyo, Japan.|CREST, Japan Science and Technology Agency, Tokyo, Japan.
authors.forename: Kensaku
authors.initials: K
authors.lastname: Mori
authors.affiliation: CREST, Japan Science and Technology Agency, Kobe, Japan.|Department of Neural Regeneration and Cell Communication, Mie University Graduate School of Medicine, Tsu, Mie, 514-8507, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Mizoguchi
authors.affiliation: Division of Molecular and Cellular Biology, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe, Hyogo, 650-0017, Japan.|Division of Pathogenetic Signaling, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe, Hyogo, 650-0047, Japan.|CREST, Japan Science and Technology Agency, Kobe, Japan.
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Takai
mesh_terms: D000818:Animals; D001710:Biotin / Q000031:analogs & derivatives; D002448:Cell Adhesion / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D003712:Dendrites / Q000378:metabolism* / Q000648:ultrastructure; D003911:Dextrans; D006624:Hippocampus / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D021621:Imaging, Three-Dimensional; D015151:Immunoblotting; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D016253:Microscopy, Immunoelectron; D000075983:Nectins; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009830:Olfactory Bulb / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D015815:Cell Adhesion Molecules; D003911:Dextrans; C000619015:Nectin1 protein, mouse; D000075983:Nectins; C076397:biotinylated dextran amine; D001710:Biotin
keywords: RRID: AB_2077527; RRID: AB_257881; RRID: AB_301787; RRID: AB_476737; RRID: AB_476831; RRID: AB_477617; RRID: nif-0000-00262; RRID: nif-0000-30467; dendritic contacts; dendrodendritic reciprocal synapses; external plexiform layer (EPL); granule cells; mitral cell lateral dendrites