title: Isolation, purification and structural characterization of polysaccharide from Acanthopanax brachypus.
abstract: A water-soluble polysaccharide, designated as ABPS-21, was isolated from the stem barks of Acanthopanax brachypus by hot-water extraction and purified by Cellulose DEAE-52 and Sephacryl S-300 HR gel-filtration chromatography, respectively. The homogeneity and molecular weight were determined using HPGPC. The structure was elucidated based on monosaccharide composition and methylation analysis, partial acid hydrolysis, periodate oxidation, Smith degradation, IR and NMR spectroscopy. The result showed that ABPS-21 was a homogeneous heteropolysaccharide including galactose, glucose, rhamnose and galacturonic acid in a molar ratio of 3.0:2.0:2.0:1.0, respectively, with an average molecular weight of 1.06 × 10(5)Da. The main chain of ABPS-21 was made up of → 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-(1 →, and two side chains α-l-Rhap-(1 → 4)-β-D-Glcp-(1 → and α-D-galpA-(1 → were attached to the backbone chain at O-6 position of 1,4,6-linked β-D-Glcp and 1,4,6-linked β-D-Galp, respectively. ABPS-21, an acidic branched polysaccharide from A. brachypus, was not described up to now.
authors.affiliation: College of Chemistry & Chemical Engineering, Longdong University, Qingyang 745000, PR China. Electronic address: hhb-88@126.com.
authors.forename: Haobin
authors.initials: H
authors.lastname: Hu
authors.affiliation: Department of Oncology, Qingyang First People's Hospital, Qingyang 745000, PR China.
authors.forename: Haipeng
authors.initials: H
authors.lastname: Liang
authors.affiliation: College of Chemistry & Chemical Engineering, Longdong University, Qingyang 745000, PR China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wu
mesh_terms: D002240:Carbohydrate Sequence; D028001:Eleutherococcus / Q000737:chemistry*; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006603:Hexuronic Acids / Q000032:analysis; D008969:Molecular Sequence Data; D018547:Plant Stems / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry*; D012210:Rhamnose / Q000032:analysis
chemical_list: D006603:Hexuronic Acids; D011134:Polysaccharides; C007819:galacturonic acid; D005947:Glucose; D012210:Rhamnose; D005690:Galactose
keywords: Acanthopanax brachypus; Isolation; Polysaccharide; Pruification; Structural characterization