title: PPARγ inhibits ovarian cancer cells proliferation through upregulation of miR-125b.
abstract: miR-125b has essential roles in coordinating tumor proliferation, angiogenesis, invasiveness, metastasis and chemotherapy recurrence. In ovarian cancer miR-125b has been shown to be downregulated and acts as a tumor suppressor by targeting proto-oncogene BCL3. PPARγ, a multiple functional transcription factor, has been reported to have anti-tumor effects through inhibition of proliferation and induction of differentiation and apoptosis by targeting the tumor related genes. However, it is unclear whether miR-125b is regulated by PPARγ in ovarian cancer. In this study, we demonstrated that the miR-125b downregulated in ovarian cancer tissues and cell lines. Ligands-activated PPARγ suppressed proliferation of ovarian cancer cells and this PPARγ-induced growth inhibition is mediated by the upregulation of miR-125b. PPARγ promoted the expression of miR-125b by directly binding to the responsive element in miR-125b gene promoter region. Thus, our results suggest that PPARγ can induce growth suppression of ovarian cancer by upregulating miR-125b which inhibition of proto-oncogene BCL3. These findings will extend our understanding of the function of PPARγ in tumorigenesis and miR-125b may be a therapeutic intervention of ovarian cancer.
authors.affiliation: Department of Obstetrics and Gynecology, Suining Central Hospital, Suining, China. Electronic address: luoshuangsch@163.com.
authors.forename: Shuang
authors.initials: S
authors.lastname: Luo
authors.affiliation: Department of Gynecology and Obsterics, Jinan Central Hospital, Jinan, China.
authors.forename: Jidong
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Otorhinolaryngolgy, Suining Central Hospital, Suining, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Gynecology and Obstetrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
authors.forename: Zhenwei
authors.initials: Z
authors.lastname: Yao
authors.affiliation: Department of Gynecology and Obsterics, Zhongshan Hospital, Wuhan, China.
authors.forename: Hongjuan
authors.initials: H
authors.lastname: Pan
mesh_terms: D000081242:B-Cell Lymphoma 3 Protein; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D047495:PPAR gamma / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000037:antagonists & inhibitors / Q000235:genetics; D011519:Proto-Oncogenes; D050436:Regulatory Elements, Transcriptional; D014157:Transcription Factors / Q000037:antagonists & inhibitors / Q000235:genetics; D015854:Up-Regulation
chemical_list: D000081242:B-Cell Lymphoma 3 Protein; C000631344:BCL3 protein, human; C000715470:MAS1 protein, human; C531888:MIRN125 microRNA, human; D035683:MicroRNAs; D047495:PPAR gamma; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D014157:Transcription Factors
keywords: Ovarian cancer; PPARγ; Proliferation; miR-125b