title: Anorexigenic effect of serotonin is associated with changes in hypothalamic nuclei activity in an avian model.
abstract: The anorexigenic effect of serotonin (5HT) has been documented for decades; however, its central mechanism has not been fully elucidated, especially so in non-mammalian vertebrates. Therefore, we centrally injected 5HT to chicks and measured several appetite-associated parameters. Chicks that received central 5HT dose- and time-dependently decreased food intake while water intake was not affected. To determine which hypothalamic nuclei were associated with this effect c-Fos immunoreactivity was measured in appetite-associated nuclei. Only the ventromedial hypothalamus and arcuate nucleus were activated. Whole blood glucose was measured after 5HT injection but was not affected. From the hypothalamus, several appetite-associated mRNAs were measured by real-time PCR after 5HT injection but not one of these showed any difference in expression. Lastly, a comprehensive behavior analysis demonstrated that 5HT caused reducing pecking and increased deep rest. Together we interpret these results as exogenous 5HT injection causes short term satiety that is likely a secondary effect to an increase in the amount of time spent in deep rest.
authors.affiliation: Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg, VA 24061, United States.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg, VA 24061, United States.
authors.forename: Dillon
authors.initials: D
authors.lastname: Didehvar
authors.affiliation: Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg, VA 24061, United States.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg, VA 24061, United States.
authors.forename: Jiaqing
authors.initials: J
authors.lastname: Yi
authors.affiliation: Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg, VA 24061, United States.
authors.forename: Elizabeth R
authors.initials: ER
authors.lastname: Gilbert
authors.affiliation: Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg, VA 24061, United States. Electronic address: macline2@vt.edu.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Cline
mesh_terms: D000818:Animals; D001066:Appetite / Q000187:drug effects; D001067:Appetite Depressants / Q000494:pharmacology*; D001069:Appetite Regulation / Q000187:drug effects; D001522:Behavior, Animal / Q000187:drug effects; D002645:Chickens / Q000378:metabolism*; D004326:Drinking / Q000187:drug effects*; D004435:Eating / Q000187:drug effects*; D007031:Hypothalamus / Q000187:drug effects* / Q000378:metabolism; D007276:Injections, Intraventricular; D023421:Models, Animal; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012701:Serotonin / Q000494:pharmacology*; D017366:Serotonin Receptor Agonists / Q000494:pharmacology*
chemical_list: D001067:Appetite Depressants; D016760:Proto-Oncogene Proteins c-fos; D017366:Serotonin Receptor Agonists; D012701:Serotonin
keywords: 5HT; Chick; Hypothalamus