title: Features for instantaneous emissions of low-level infrared signals of glucokinase enzyme from Pyrococcus furiosus.
abstract: A noncontact infrared (IR) imaging-based methodology and signal recovery tools are applied on an enzyme reaction as a test target. The method is implemented by a long-wave (8-12 μm) IR microbolometer imaging array and a germanium-based IR optical vision. The reaction is carried out by the glucokinase, which produces a rapid exothermal release of energy that is weak, and, even worse, the IR video captured by the uncooled microbolometer detector is affected by spatial and temporal noise with specific complexities. Hitherto, IR-based signal recovery tools have worked with a standard acquisition frequency, which is clearly beyond the time scale of a real scenario. The implications of this (and similar) rapid reactions motivate the designs of a signal recovery method using prior information of the processes to extract and quantify the spontaneity of the enzymatic reaction in a three-dimensional (space and time) single and noncontact online measurement.
authors.forename: Sergio
authors.initials: S
authors.lastname: Torres
authors.forename: Héctor
authors.initials: H
authors.lastname: Mella
authors.forename: Claudio
authors.initials: C
authors.lastname: Reyes
authors.forename: Pablo
authors.initials: P
authors.lastname: Meza
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Gallardo
authors.forename: Juan P
authors.initials: JP
authors.lastname: Staforelli
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry; D002138:Calibration; D004581:Electronics; D004867:Equipment Design; D005941:Glucokinase / Q000737:chemistry*; D006358:Hot Temperature; D007259:Infrared Rays; D016011:Normal Distribution; D055096:Optical Devices; D055620:Optical Phenomena; D020027:Pyrococcus furiosus / Q000201:enzymology*; D013055:Spectrophotometry, Infrared / Q000295:instrumentation* / Q000379:methods
chemical_list: D000255:Adenosine Triphosphate; D005941:Glucokinase