title: Iron and FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR-dependent regulation of proteins and genes in Arabidopsis thaliana roots.
abstract: Iron is an essential micronutrient for plants, and iron deficiency requires a variety of physiological adaptations. FIT (FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR) is essential for the regulation of iron uptake in Arabidopsis thaliana roots. FIT is transcriptionally as well as posttranscriptionally regulated in response to iron supply. To investigate to which extent posttranscriptional regulation upon iron deficiency applies to proteins and to determine the dependency on FIT, we performed a parallel proteomic and transcriptomic study with wild-type, a fit knock-out mutant, and a FIT overexpressing Arabidopsis line. Among 92 proteins differentially regulated by iron and/or FIT, we identified 30 proteins, which displayed differential regulation at the transcriptional level. Eleven protein spots were regulated in at least one of the data points even contrary to the respective genes dependent on FIT. We found ten proteins in at least two forms. The analysis of functional classification showed enriched GO terms among the posttranscriptionally regulated genes and of proteins, that were downregulated or absent in the fit knock-out mutant. Taken together, we provide evidence for iron and FIT-dependent posttranscriptional regulation in iron homeostasis in A. thaliana.
authors.affiliation: Institute of Botany, Heinrich Heine University Düsseldorf, Düsseldorf, Germany.
authors.forename: Hans-Jörg
authors.initials: HJ
authors.lastname: Mai
authors.affiliation: Helmholtz Zentrum München (GmbH), German Research Center for Environmental Health, Institute of Biochemical Plant Pathology (BIOP), Neuherberg, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Lindermayr
authors.affiliation: Research Unit Protein Science, Helmholtz Zentrum München (GmbH), German Research Center for Environmental Health, Neuherberg, Germany.
authors.forename: Christine
authors.initials: C
authors.lastname: von Toerne
authors.affiliation: Leibniz-Institute for New Materials, Saarbrücken, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Fink-Straube
authors.affiliation: Helmholtz Zentrum München (GmbH), German Research Center for Environmental Health, Institute of Biochemical Plant Pathology (BIOP), Neuherberg, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Durner
authors.affiliation: Institute of Botany, Heinrich Heine University Düsseldorf, Düsseldorf, Germany.|CEPLAS Cluster of Excellence on Plant Sciences, Heinrich Heine Universität Düsseldorf, Düsseldorf, Germany.
authors.forename: Petra
authors.initials: P
authors.lastname: Bauer
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D018506:Gene Expression Regulation, Plant; D055786:Gene Knockout Techniques; D007501:Iron / Q000378:metabolism*; D009154:Mutation; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D029681:Arabidopsis Proteins; D051792:Basic Helix-Loop-Helix Proteins; D007501:Iron; C501411:FIT1 protein, Arabidopsis
keywords: Arabidopsis; FIT; Iron; Plant proteomics; Posttranscriptional regulation; Transcription