title: Sample holder for axial rotation of specimens in 3D microscopy.
abstract: In common light microscopy, observation of samples is only possible from one perspective. However, especially for larger three-dimensional specimens observation from different views is desirable. Therefore, we are presenting a sample holder permitting rotation of the specimen around an axis perpendicular to the light path of the microscope. Thus, images can be put into a defined multidimensional context, enabling reliable three-dimensional reconstructions. The device can be easily adapted to a great variety of common light microscopes and is suitable for various applications in science, education and industry, where the observation of three-dimensional specimens is essential. Fluorescence z-projection images of copepods and ixodidae ticks at different rotation angles obtained by confocal laser scanning microscopy and light sheet fluorescence microscopy are reported as representative results.
authors.affiliation: Aalen University, Institute of Applied Research, Beethovenstr. 1, 73430, Aalen, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Bruns
authors.affiliation: Aalen University, Institute of Applied Research, Beethovenstr. 1, 73430, Aalen, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Schickinger
authors.affiliation: Aalen University, Institute of Applied Research, Beethovenstr. 1, 73430, Aalen, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Schneckenburger
mesh_terms: D000818:Animals; D033342:Copepoda / Q000648:ultrastructure; D005453:Fluorescence; D021621:Imaging, Three-Dimensional / Q000295:instrumentation* / Q000379:methods; D026863:Ixodidae / Q000648:ultrastructure; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D012399:Rotation
keywords: 3D imaging; SPIM; axial tomography; confocal laser scanning microscopy; light sheet fluorescence microscopy; microcapillary