title: Bio301: A Web-Based EST Annotation Pipeline That Facilitates Functional Comparison Studies.
abstract: In this postgenomic era, a huge volume of information derived from expressed sequence tags (ESTs) has been constructed for functional description of gene expression profiles. Comparative studies have become more and more important to researchers of biology. In order to facilitate these comparative studies, we have constructed a user-friendly EST annotation pipeline with comparison tools on an integrated EST service website, Bio301. Bio301 includes regular EST preprocessing, BLAST similarity search, gene ontology (GO) annotation, statistics reporting, a graphical GO browsing interface, and microarray probe selection tools. In addition, Bio301 is equipped with statistical library comparison functions using multiple EST libraries based on GO annotations for mining meaningful biological information.
authors.affiliation: Institute of Information Science, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei 115, Taiwan.
authors.forename: Yen-Chen
authors.initials: YC
authors.lastname: Chen
authors.affiliation: Department of Biomedical Engineering, The Whitaker Biomedical Engineering Institute at Johns Hopkins University School of Medicine, 720 Rutland Avenue, Baltimore, MD 21205, USA.
authors.forename: Yun-Ching
authors.initials: YC
authors.lastname: Chen
authors.affiliation: Institute of Plant and Microbial Biology, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei 115, Taiwan.
authors.forename: Wen-Dar
authors.initials: WD
authors.lastname: Lin
authors.affiliation: Department of Bioscience Technology, Chung Yuan Christian University, 200 Chung Pei Road, Chung Li City 32073, Taiwan.
authors.forename: Chung-Der
authors.initials: CD
authors.lastname: Hsiao
authors.affiliation: Graduate Institute of Biomedical Informatics, Taipei Medical University, 250 Wu-Hsing Street, Taipei City 110, Taiwan.
authors.forename: Hung-Wen
authors.initials: HW
authors.lastname: Chiu
authors.affiliation: Institute of Information Science, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei 115, Taiwan.
authors.forename: Jan-Ming
authors.initials: JM
authors.lastname: Ho