title: Spatial Learning Requires mGlu5 Signalling in the Dorsal Hippocampus.
abstract: We examined the role of hippocampal metabotropic glutamate receptor 5 (mGlu5) in spatial learning and memory. Although it has been shown that mGlu5 signalling is required for certain forms of learning and memory, its role in spatial learning is unclear since studies using pharmacological or knockout mice models provide inconsistent findings. Additionally, the location in the brain where mGlu5 signalling may modulate such learning is yet to be precisely delineated. We stereotaxically injected rAAV-Cre into the dorsal hippocampus of mGlu5(loxP/loxP) mice to knockdown mGlu5 in that region. We show for the first time that knockdown of mGlu5 in the dorsal hippocampus is sufficient to impair spatial learning in Morris Water Maze. Locomotor activity and memory retrieval were unaffected by the mGlu5 knockdown. Taken together, these findings support a key role for dorsal hippocampal mGlu5 signalling in spatial learning.
authors.affiliation: Behavioural Neuroscience Division, Florey Institute of Neuroscience and Mental Health, Parkville, VIC, Australia.
authors.forename: Shawn Zheng Kai
authors.initials: SZ
authors.lastname: Tan
authors.forename: Despina E
authors.initials: DE
authors.lastname: Ganella
authors.forename: Alec Lindsay Ward
authors.initials: AL
authors.lastname: Dick
authors.forename: Jhodie R
authors.initials: JR
authors.lastname: Duncan
authors.forename: Emma
authors.initials: E
authors.lastname: Ong-Palsson
authors.forename: Ross A D
authors.initials: RA
authors.lastname: Bathgate
authors.forename: Jee Hyun
authors.initials: JH
authors.lastname: Kim
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Lawrence
mesh_terms: D000256:Adenoviridae; D000818:Animals; D015870:Gene Expression; D005822:Genetic Vectors; D006624:Hippocampus / Q000502:physiology*; D019426:Integrases / Q000235:genetics; D018782:Maze Learning / Q000502:physiology*; D008568:Memory / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008845:Microinjections; D009043:Motor Activity / Q000502:physiology; D064529:Receptor, Metabotropic Glutamate 5 / Q000235:genetics / Q000502:physiology*
chemical_list: C578936:Grm5 protein, mouse; D064529:Receptor, Metabotropic Glutamate 5; C045073:Cre recombinase; D019426:Integrases