title: Fasting for 3 days during the suckling-weaning transient period in male rats induces metabolic abnormalities in the liver and is associated with impaired glucose tolerance in adulthood.
abstract: OBJECTIVE
  Recent studies suggest that nutritional status during developmental periods is associated with subsequent development of metabolic abnormalities. In this study, we examined whether malnutrition by fasting for 3 days during the suckling-weaning transient period induces subsequent development of metabolic abnormalities in rats.
  
  
  METHODS
  Male Sprague-Dawley rats were fasted for 3 days during the suckling-weaning transient period. They are subsequently fed a high-fat, high-sucrose (HF) or low-fat, high-starch (LF) diet for 14 weeks from 17 weeks of age, and the liver and blood samples were collected for measuring mRNA and protein levels of metabolic genes and blood concentrations of glucose and insulin, respectively.
  
  
  RESULTS
  Fasting for 3 days during the suckling-weaning transient period induced impaired glucose tolerance in rats fed the LF diet in adulthood. Liver triglycerides in rats fed the HF diet in adulthood increased to 140 % in rats fasted for 3 days during the suckling-weaning transient period compared with those non-fasted. Furthermore, liver expression of FBP1 and ACCα genes in adult rats fed the LF diet increased to 125 and 145 %, respectively, in rats fasted for 3 days during the suckling-weaning transient period compared to non-fasted rats. PEPCK1 protein expression levels in rats fed the LF diet were higher in rats fasted for 3 days during the suckling-weaning transient period than in non-fasted rats.
  
  
  CONCLUSIONS
  Fasting for 3 days in rats during the suckling-weaning transient period enhances metabolic abnormalities in animals fed a HF or LF diet in adulthood by confounding metabolism of lipid and sugar in the liver.
authors.affiliation: Laboratory of Nutritional Physiology, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, Shizuoka, 422-8526, Japan.
authors.forename: Misa
authors.initials: M
authors.lastname: Ikeda
authors.affiliation: Laboratory of Nutritional Physiology, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, Shizuoka, 422-8526, Japan.
authors.forename: Kazue
authors.initials: K
authors.lastname: Honma
authors.affiliation: Laboratory of Nutritional Physiology, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, Shizuoka, 422-8526, Japan.|Laboratory of Food and Nutritional Sciences, Department of Local Produce and Food Sciences, Faculty of Life and Environmental Sciences, University of Yamanashi, Yamanashi, 400-8510, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Mochizuki
authors.affiliation: Laboratory of Nutritional Physiology, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, Shizuoka, 422-8526, Japan. gouda@u-shizuoka-ken.ac.jp.
authors.forename: Toshinao
authors.initials: T
authors.lastname: Goda
mesh_terms: D052242:Adiponectin / Q000097:blood; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D000833:Animals, Suckling; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D059305:Diet, High-Fat; D004041:Dietary Fats; D004195:Disease Models, Animal; D005215:Fasting; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005943:Gluconeogenesis / Q000235:genetics; D018149:Glucose Intolerance; D007328:Insulin / Q000097:blood; D020738:Leptin / Q000097:blood; D050155:Lipogenesis / Q000235:genetics; D008099:Liver / Q000378:metabolism / Q000503:physiopathology*; D008297:Male; D044342:Malnutrition / Q000150:complications / Q000503:physiopathology; D008659:Metabolic Diseases / Q000209:etiology / Q000503:physiopathology*; D009929:Organ Size; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014280:Triglycerides / Q000097:blood; D014886:Weaning
chemical_list: D052242:Adiponectin; D001786:Blood Glucose; D004268:DNA-Binding Proteins; D004041:Dietary Fats; C000588995:FBP1 protein, rat; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D020738:Leptin; D012333:RNA, Messenger; D014280:Triglycerides
keywords: Impaired glucose tolerance; Malnutrition; Metabolic abnormalities; Suckling–weaning transient period