title: Acceleration of callus formation during fracture healing using basic fibroblast growth factor-kidney disease domain-collagen-binding domain fusion protein combined with allogenic demineralized bone powder.
abstract: BACKGROUND
  To repair fractures with large bone defects or gaps, demineralized allogenic bone matrix (DBM) is often applied to the fracture site. However, studies have shown that the use of DBM alone has limited efficacy for repairing fractures. In the present study, we developed an allogenic demineralized bone powder (DBP) with basic fibroblast-derived growth factor containing a polycystic kidney disease (PKD) domain and collagen-binding domain (CBD) from Clostridium histolyticum collagenase (ColH) and investigated the stimulatory effects of bFGF-PKD-CBD combined with allogenic DBP on bone growth in a mouse femur fracture model.
  
  
  METHODS
  DBP mixed with either phosphate-buffered saline (PBS) (DBP/PBS), 0.58 nmol basic fibroblast growth factor (bFGF) (0.58 nmol DBP/bFGF), 0.058 nmol bFGF-PKD-CBD (0.058 nmol DBP/bFGF-PKD-CBD), or 0.58 nmol bFGF-PKD-CBD (0.58 nmol DBP/bFGF-PKD-CBD) was grafted into fracture sites.
  
  
  RESULTS
  bFGF-PKD-CBD/DBP composite accelerates callus formation in a bone fracture model in mice and clearly showed that the composite also increases bone mineral density at fracture sites compared to bFGF/DBP. In addition, bFGF-PKD-CBD/DBP increased callus volume and bone mineral content to similar levels in fractures treated with a tenfold higher amount of bFGF at 4 weeks.
  
  
  CONCLUSIONS
  Our results suggest that bFGF-PKD-CBD/DBP may be useful for promoting fracture healing in the clinical setting.
authors.affiliation: Department of Orthopaedic Surgery, Kitasato University School of Medicine, 1-15-1 Minami-ku Kitasato, Sagamihara, Kanagawa, Japan. boatwataru@hotmail.co.jp.
authors.forename: Wataru
authors.initials: W
authors.lastname: Saito
authors.affiliation: Department of Orthopaedic Surgery, Kitasato University School of Medicine, 1-15-1 Minami-ku Kitasato, Sagamihara, Kanagawa, Japan. kuchida@med.kitasato-u.ac.jp.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Uchida
authors.affiliation: Department of Bacteriology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Kita-ku Shikata-cho, Okayama, Japan. osamu@okayama-u.ac.jp.
authors.forename: Osamu
authors.initials: O
authors.lastname: Matsushita
authors.affiliation: Department of Orthopaedic Surgery, Kitasato University School of Medicine, 1-15-1 Minami-ku Kitasato, Sagamihara, Kanagawa, Japan. ginoue-ktz@umin.org.
authors.forename: Gen
authors.initials: G
authors.lastname: Inoue
authors.affiliation: Department of Orthopaedic Surgery, Kitasato University School of Medicine, 1-15-1 Minami-ku Kitasato, Sagamihara, Kanagawa, Japan. guccyon_s@yahoo.co.jp.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sekiguchi
authors.affiliation: Department of Orthopaedic Surgery, Kitasato University School of Medicine, 1-15-1 Minami-ku Kitasato, Sagamihara, Kanagawa, Japan. jun43814@gmail.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Aikawa
authors.affiliation: Department of Orthopaedic Surgery, Kitasato University School of Medicine, 1-15-1 Minami-ku Kitasato, Sagamihara, Kanagawa, Japan. hisako19830608@yahoo.co.jp.
authors.forename: Hisako
authors.initials: H
authors.lastname: Fujimaki
authors.affiliation: Department of Orthopaedic Surgery, Kitasato University School of Medicine, 1-15-1 Minami-ku Kitasato, Sagamihara, Kanagawa, Japan. mtakaso@kitasato-u.ac.jp.
authors.forename: Masashi
authors.initials: M
authors.lastname: Takaso
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D016025:Bone Transplantation / Q000379:methods*; D002146:Bony Callus / Q000187:drug effects*; D002352:Carrier Proteins / Q000494:pharmacology / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D005264:Femoral Fractures / Q000628:therapy*; D016222:Fibroblast Growth Factor 2; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D011993:Recombinant Fusion Proteins; D014184:Transplantation, Homologous
chemical_list: D002352:Carrier Proteins; D011993:Recombinant Fusion Proteins; C000605742:basic fibroblast growth factor-kidney disease domain-collagen-binding domain fusion protein, mouse; D016222:Fibroblast Growth Factor 2