title: Asymmetric Biocatalytic Amination of Ketones at the Expense of NH3 and Molecular Hydrogen.
abstract: A biocatalytic system is presented for the stereoselective amination of ketones at the expense of NH3 and molecular hydrogen. By using a NAD(+)-reducing hydrogenase, an alanine dehydrogenase, and a suitable ω-transaminase, the R- as well as the S-enantiomer of various amines could be prepared with up to >99% ee and 98% conversion.
authors.affiliation: †Department of Chemistry, University of Graz, NAWI Graz, Heinrichstraße 28, 8010 Graz, Austria.
authors.forename: Anja K
authors.initials: AK
authors.lastname: Holzer
authors.affiliation: †Department of Chemistry, University of Graz, NAWI Graz, Heinrichstraße 28, 8010 Graz, Austria.
authors.forename: Katharina
authors.initials: K
authors.lastname: Hiebler
authors.affiliation: †Department of Chemistry, University of Graz, NAWI Graz, Heinrichstraße 28, 8010 Graz, Austria.
authors.forename: Francesco G
authors.initials: FG
authors.lastname: Mutti
authors.affiliation: †Department of Chemistry, University of Graz, NAWI Graz, Heinrichstraße 28, 8010 Graz, Austria.
authors.forename: Robert C
authors.initials: RC
authors.lastname: Simon
authors.affiliation: ‡Department of Chemistry, Technische Universität Berlin, Straße des 17. Juni 135, 10623 Berlin, Germany.
authors.forename: Lars
authors.initials: L
authors.lastname: Lauterbach
authors.affiliation: ‡Department of Chemistry, Technische Universität Berlin, Straße des 17. Juni 135, 10623 Berlin, Germany.
authors.forename: Oliver
authors.initials: O
authors.lastname: Lenz
authors.affiliation: †Department of Chemistry, University of Graz, NAWI Graz, Heinrichstraße 28, 8010 Graz, Austria.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Kroutil
mesh_terms: D000586:Amination; D000588:Amines / Q000737:chemistry / Q000378:metabolism*; D000641:Ammonia / Q000737:chemistry / Q000378:metabolism*; D055162:Biocatalysis; D006859:Hydrogen / Q000737:chemistry / Q000378:metabolism*; D006864:Hydrogenase / Q000378:metabolism*; D007659:Ketones / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure
chemical_list: D000588:Amines; D007659:Ketones; D000641:Ammonia; D006859:Hydrogen; D006864:Hydrogenase