title: Development of Gd(III) porphyrin-conjugated chitosan nanoparticles as contrast agents for magnetic resonance imaging.
abstract: A novel magnetic resonance imaging (MRI) contrast agent based on gadolinium meso-tetrakis(4-pyridyl)porphyrin [Gd(TPyP)] conjugated with chitosan nanoparticles has been developed. The chitosan nanoparticles were synthesized following an ionic gelation method and the conditions optimized to generate small nanoparticles (CNs) with a narrow size distribution of 35-65 nm. The gadolinium meso-tetrakis(4-pyridyl)porphyrin [Gd(TPyP)] was loaded into chitosan nanoparticles by passive adsorption. The interaction of chitosan with Gd(TPyP) has been examined by UV-visible, Fourier transform infrared spectroscopies (FT-IR) and inductively coupled plasma mass spectrometry (ICP-MS), which indicate the successful association of Gd(TPyP) without any structural distortion throughout the chitosan nanoparticles. The potential of Gd(TPyP)-CNs as MRI contrast agent has been investigated by magnetic resonance imaging (MRI) in-vitro. Relaxivities of Gd(TPyP)-CNs obtained from T1-weighted images, increased with Gd concentration and attained an optimum r1 of 38.35 mM(-1) s(-1), which is 12-fold higher compared to commercial Gd-DOTA (~4 mM(-1) s(-1) at 3T). The combination of such strong MRI contrast with the known properties of porphyrins in photodynamic therapy and biocompatibility of chitosan, presents a new perspective in using these compounds in cancer theranostics.
authors.affiliation: Université Paul Sabatier, Toulouse III, INSERM U825, CHU Purpan, 31059 Toulouse Cedex 9, France.
authors.forename: Tania
authors.initials: T
authors.lastname: Jahanbin
authors.affiliation: Institut de Chimie Moléculaire et des Matériaux d'Orsay, UMR CNRS 8182, ECBB, Université Paris-Sud, 91405 Orsay, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Sauriat-Dorizon
authors.affiliation: Faculty of Science, Engineering and Computing, University of Kingston, Penrhyn Road Kingston upon Thames Surrey KT1 2EE, London, UK.
authors.forename: Peter
authors.initials: P
authors.lastname: Spearman
authors.affiliation: Université Paul Sabatier, Toulouse III, INSERM U825, CHU Purpan, 31059 Toulouse Cedex 9, France. Electronic address: soraya.benderbous@univ-tlse3.fr.
authors.forename: Soraya
authors.initials: S
authors.lastname: Benderbous
authors.affiliation: Institut de Chimie Moléculaire et des Matériaux d'Orsay, UMR CNRS 8182, ECBB, Université Paris-Sud, 91405 Orsay, France. Electronic address: hafsa.korri-youssoufi@u-psud.fr.
authors.forename: Hafsa
authors.initials: H
authors.lastname: Korri-Youssoufi
mesh_terms: D003287:Contrast Media / Q000737:chemistry*; D005682:Gadolinium / Q000737:chemistry*; D008279:Magnetic Resonance Imaging; D053758:Nanoparticles / Q000737:chemistry*
chemical_list: D003287:Contrast Media; D005682:Gadolinium
keywords: Chitosan nanoparticles; Dynamic light scattering; Gadolinium porphyrin; Passive loading; Relaxivity