title: Expression of AQP3 protein in hAECs is regulated by Camp-PKA-CREB signalling pathway.
abstract: Previous studies by others and our group have demonstrated the expression of AQP3 protein in human chorioamniotic membranes. Here, we investigated whether cyclic adenosine monophosphate (cAMP) up-regulation of aquaporin 3 (AQP3) protein expression in human amniotic epithelial cells (hAECs) is mediated by protein kinase A (PKA) dependent or independent pathway. Cells were treated with various concentrations of Forskolin, SP-cAMP, H-89 at various time intervals or optimal concentration of Forskolin in combination with H-89 in the blocking experiments. Forskolin significantly increased cAMP levels and the expression of PKA, p-CREB and AQP3. SP-cAMP had similar effects. H-89 inhibited PKA, p-CREB and AQP3 protein expression, and attenuated the stimulatory effect of Forskolin. These results show that the AQP3 protein expression in hAECs was regulated by cAMP-PKA-CREB signal pathway. A relatively short biological half life of AQP3 renders its rapid responsiveness to regulation.
authors.forename: Ying
authors.initials: Y
authors.lastname: Hua
authors.affiliation: fbs.
authors.forename: Shengdi
authors.initials: S
authors.lastname: Ding
authors.forename: Wenwen
authors.initials: W
authors.lastname: Zhang
authors.forename: Qingfeng
authors.initials: Q
authors.lastname: Zhou
authors.forename: Weijing
authors.initials: W
authors.lastname: Ye
authors.forename: Miaomiao
authors.initials: M
authors.lastname: Chen
authors.affiliation: Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, China, zjwzzxq@yeah.net.
authors.forename: Xueqiong
authors.initials: X
authors.lastname: Zhu
mesh_terms: D051400:Aquaporin 3 / Q000235:genetics / Q000378:metabolism*; D049109:Cell Proliferation; D002478:Cells, Cultured; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism / Q000502:physiology*; D005786:Gene Expression Regulation; D006801:Humans; D007546:Isoquinolines / Q000494:pharmacology; D015398:Signal Transduction; D013449:Sulfonamides / Q000494:pharmacology; D015854:Up-Regulation
chemical_list: C496027:AQP3 protein, human; C115163:CREB1 protein, human; D017362:Cyclic AMP Response Element-Binding Protein; D007546:Isoquinolines; D013449:Sulfonamides; D051400:Aquaporin 3; D005576:Colforsin; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases; C063509:N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide