title: Isolation, structural characterization and neurotrophic activity of a polysaccharide from Phellinus ribis.
abstract: A new polysaccharide named PRG was isolated from the fruiting bodies of Phellinus ribis by hot water extraction, ethanol precipitation, anion-exchange and gel-filtration chromatography. PRG was homogeneous, with a molecular weight of 5.16 × 10(3)Da, as determined by high-performance size-exclusion chromatography-multiangle laser light scattering analysis. Its structural characteristics were investigated and elucidated by methylation analysis, partial acid hydrolysis, gas-liquid chromatography mass spectrometry, Fourier transform infrared and nuclear magnetic resonance spectroscopy. Based on obtained data, PRG was found to be a β-d-glucan containing a (1 → 3)-linked backbone, with a branch of two (1 → 6)-linked and one terminal glucoses substituting at the C-6 position every three residues, along the main chain. PRG exhibited neurotrophic activity, which significantly promoted the neurite outgrowth of the nerve growth factor-stimulated PC12 cells, suggesting that it might be a potential candidate for the treatment of neurodegenerative diseases.
authors.affiliation: School of Pharmaceutical Sciences, Shandong University of Traditional Chinese Medicine, Jinan 250355, China; Institute of Biochemical and Biotechnological Drug, Key Laboratory of Chemical Biology of Natural Products (Ministry of Education), School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Biochemical and Biotechnological Drug, Key Laboratory of Chemical Biology of Natural Products (Ministry of Education), School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Liu
authors.affiliation: School of Pharmaceutical Sciences, Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
authors.forename: Haiqiang
authors.initials: H
authors.lastname: Jiang
authors.affiliation: School of Pharmaceutical Sciences, Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
authors.forename: Honglei
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Institute of Clinical Pharmacology, Qilu Hospital of Shandong University, Jinan 250012, China.
authors.forename: Pingli
authors.initials: P
authors.lastname: Li
authors.affiliation: Institute of Biochemical and Biotechnological Drug, Key Laboratory of Chemical Biology of Natural Products (Ministry of Education), School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China. Electronic address: fswang@sdu.edu.cn.
authors.forename: Fengshan
authors.initials: F
authors.lastname: Wang
mesh_terms: D000818:Animals; D001487:Basidiomycota / Q000737:chemistry*; D002240:Carbohydrate Sequence; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000494:pharmacology; D005947:Glucose / Q000032:analysis; D008969:Molecular Sequence Data; D016501:Neurites / Q000187:drug effects*; D016716:PC12 Cells; D051381:Rats
chemical_list: D062610:Fungal Polysaccharides; D005947:Glucose
keywords: Neurotrophic activity; Phellinus ribis; Structure; d-Glucan