title: Nitric oxide-releasing chitosan film for enhanced antibacterial and in vivo wound-healing efficacy.
abstract: Nitric oxide (NO) is a promising therapeutic agent with antibacterial and wound-healing properties. However, the gaseous state and short half-life of NO necessitate a formulation that can control its storage and release. In this study, we developed NO-releasing films (CS/NO film) composed of chitosan (CS) and S-nitrosoglutathione (GSNO) as a NO donor. Thermal analysis demonstrated molecular dispersion of GSNO in the films. In vitro release study revealed that NO release from CS/NO films followed Korsmeyer-Peppas model with Fickian diffusion kinetics. Moreover, the CS/NO film showed a stronger antibacterial activity against Pseudomonas aeruginosa (Gram-negative) and Staphylococcus aureus (Gram-positive) than the CS film. Further, the CS/NO film accelerated wound healing and epithelialization in a rat model of full-thickness wounds as compared to the CS film. Histopathological studies revealed that CS/NO films favorably enhanced the re-epithelialization and reconstruction of wounded skin. Therefore, our results suggest that CS/NO films could be a suitable formulation for treating full-thickness wounds.
authors.affiliation: College of Pharmacy, Yeungnam University, Gyeongsan 712-749, South Korea.
authors.forename: Jong Oh
authors.initials: JO
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Pusan National University, Busan 609-735, South Korea.
authors.forename: Jin-Ki
authors.initials: JK
authors.lastname: Noh
authors.affiliation: College of Pharmacy, Yeungnam University, Gyeongsan 712-749, South Korea.
authors.forename: Raj Kumar
authors.initials: RK
authors.lastname: Thapa
authors.affiliation: College of Pharmacy, Pusan National University, Busan 609-735, South Korea.
authors.forename: Nurhasni
authors.initials: N
authors.lastname: Hasan
authors.affiliation: College of Pharmacy, Pusan National University, Busan 609-735, South Korea.
authors.forename: Moonjeong
authors.initials: M
authors.lastname: Choi
authors.affiliation: College of Pharmacy, Yeungnam University, Gyeongsan 712-749, South Korea.
authors.forename: Jeong Hwan
authors.initials: JH
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Pusan National University, Busan 609-735, South Korea.
authors.forename: Joon-Hee
authors.initials: JH
authors.lastname: Lee
authors.affiliation: College of Korean Medicine, Daegu Haany University, Gyeongsan 712-715, South Korea.
authors.forename: Sae Kwang
authors.initials: SK
authors.lastname: Ku
authors.affiliation: College of Pharmacy, Pusan National University, Busan 609-735, South Korea. Electronic address: jinwook@pusan.ac.kr.
authors.forename: Jin-Wook
authors.initials: JW
authors.lastname: Yoo
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D001458:Bandages; D048271:Chitosan / Q000737:chemistry*; D004058:Diffusion; D052978:Disk Diffusion Antimicrobial Tests; D065546:Drug Liberation; D007700:Kinetics; D008297:Male; D008567:Membranes, Artificial; D009569:Nitric Oxide / Q000737:chemistry* / Q000494:pharmacology; D020030:Nitric Oxide Donors / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development; D051381:Rats; D017207:Rats, Sprague-Dawley; D026422:S-Nitrosoglutathione / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D008567:Membranes, Artificial; D020030:Nitric Oxide Donors; D009569:Nitric Oxide; D026422:S-Nitrosoglutathione; D048271:Chitosan
keywords: Antibacterial activity; Nitric oxide-releasing film; Wound healing