title: A simple and sensitive electrochemical immunosensor based on thiol aromatic aldehyde as a substrate for the antibody immobilization.
abstract: In this work, a novel sulfur-containing compound with aldehyde groups was synthesized, which was used to immobilize antibodies through the covalent bonding of the aldehyde groups with amino groups, in which no additional chemical cross-linker is required. Human IgG was used as a model analyte to fabricate the electrochemical immunosensor. Using the proposed immunosensor, IgG was detected within the range from 0.01 to 25ng mL(-1) with a detection limit of 0.003ng mL(-1) obtained by 3 S/N. The simple electrochemical immunosensor had a good specificity, stability and reproducibility.
authors.affiliation: Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China; College of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde 415000, PR China. Electronic address: ymshen79@163.com.
authors.forename: Youming
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China. Electronic address: zhangyy@hunnu.edu.cn.
authors.forename: Youyu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Liu
authors.affiliation: College of Science, Hunan Agricultural University, Changsha 410128, PR China.
authors.forename: Xiaoyin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
authors.forename: Huan
authors.initials: H
authors.lastname: Guo
authors.affiliation: College of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde 415000, PR China.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde 415000, PR China.
authors.forename: Chunxiang
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Li
authors.affiliation: Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
authors.forename: Shouzhuo
authors.initials: S
authors.lastname: Yao
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry*; D001547:Benzaldehydes / Q000737:chemistry; D002138:Calibration; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D004867:Equipment Design; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis / Q000097:blood*; D057230:Limit of Detection; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000032:analysis; D008063:Thioctic Acid / Q000737:chemistry
chemical_list: D055910:Antibodies, Immobilized; D001547:Benzaldehydes; D007074:Immunoglobulin G; D012710:Serum Albumin, Bovine; D008063:Thioctic Acid; C011483:4-hydroxybenzaldehyde
keywords: Antibody immobilization; Electrochemical immunosensor; Human IgG; Synthesis; Thiol aromatic aldehyde