title: MTB-DR-RIF 9G membrane: a platform for multiplex SNP detection of multidrug-resistant TB.
abstract: The MTB-DR-RIF 9G membrane can detect by detecting multiple mutations in multiple codons. The MTB-DR-RIF 9G membrane possesses clinical applicability in point-of-care settings for the following reasons: (i) 100% similar results with that of the sequencing analysis for clinical samples, (ii) discrimination of the multiple mutations in multiple codons, (iii) a specific/non-specific hybridization ratio higher than 350:1, and (iv) the sensitivity was found to be 1-10 copies/test for detection and discrimination of the wild and mutant TB strains. Graphical abstract Schematic illustration of the effect of controller DNA on the hybridization of the immobilized probes (corresponding to the wild TB strain) with the PCR product of (a) wild TB strain and (b) mutant TB strain.
authors.affiliation: Institute for Applied Chemistry and Department of Chemistry, Hallym University, 1 Okcheon-dong, Chuncheon, 200-702, South Korea.
authors.forename: Danishmalik Rafiq
authors.initials: DR
authors.lastname: Sayyed
authors.forename: Satish Balasaheb
authors.initials: SB
authors.lastname: Nimse
authors.forename: Keum-Soo
authors.initials: KS
authors.lastname: Song
authors.forename: Nackmoon
authors.initials: N
authors.lastname: Sung
authors.forename: Taisun
authors.initials: T
authors.lastname: Kim
mesh_terms: D006801:Humans; D008567:Membranes, Artificial; D019095:Point-of-Care Systems; D020641:Polymorphism, Single Nucleotide; D018088:Tuberculosis, Multidrug-Resistant / Q000175:diagnosis* / Q000235:genetics
chemical_list: D008567:Membranes, Artificial