title: Carbon nanotube/polymer composite electrodes for flexible, attachable electrochemical DNA sensors.
abstract: All-solution-processed, easily-made, flexible multi-walled carbon nanotube (MWCNT)/polydimethylsiloxane (PDMS)-based electrodes were fabricated and used for electrochemical DNA sensors. These electrodes could serve as a recognition layer for DNA, without any surface modification, through π-π interactions between the MWCNTs and DNA, greatly simplifying the fabrication process for DNA sensors. The electrodes were directly connected to an electrochemical analyzer in the differential pulse voltammetry (DPV) and cyclic voltammetry (CV) measurements, where methylene blue was used as a redox indicator. Since neither functional groups nor probe DNA were immobilized on the surfaces of the electrodes, the sensor can be easily regenerated by washing these electrodes with water. The limit of detection was found to be 1.3 × 10(2)pM (S/N=3), with good DNA sequence differentiation ability. Fast fabrication of a DNA sensor was also achieved by cutting and attaching the MWCNT-PDMS composite electrodes at an analyte solution-containable region. Our results pave the way for developing user-fabricated easily attached DNA sensors at low costs.
authors.affiliation: Department of Bio-Nano Technology, Gachon University, Gyeonggi 461-701, Republic of Korea; Gachon Bio-Nano Research Institute, Gyeonggi 461-701, Republic of Korea.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Nano-Physics, Gachon University, Gyeonggi 461-701, Republic of Korea; Gachon Bio-Nano Research Institute, Gyeonggi 461-701, Republic of Korea. Electronic address: eclee@gachon.ac.kr.
authors.forename: Eun-Cheol
authors.initials: EC
authors.lastname: Lee
mesh_terms: D003217:Conductometry / Q000295:instrumentation*; D004247:DNA / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D055119:Elastic Modulus; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D053761:Nanocomposites / Q000737:chemistry / Q000648:ultrastructure; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000295:instrumentation*
chemical_list: D037742:Nanotubes, Carbon; D011108:Polymers; D004247:DNA
keywords: DNA sensor; Electrode; Flexible; Multi-walled carbon nanotube; Polymer