title: Oral administration of Lactobacillus casei variety rhamnosus partially alleviates TMA-induced atopic dermatitis in mice through improving intestinal microbiota.
abstract: OBJECTIVE
  The purpose of this study was to investigate the effect of Lactobacillus casei variety rhamnosus (LCR35) on Atopic dermatitis (AD)-like symptoms in mice.
  
  
  RESULTS
  AD-like skin lesions in BALB/C mice were induced by sensitization and subsequent repeated challenges with trimellitic anhydride (TMA) for 10 days. LCR35 was orally administered to the mice once daily throughout the study. In the TMA-induced AD model, orally administered LCR35 suppressed significantly irritant-related scratching behaviour and skin dehydration as well as apparent severity of AD. LCR35 also significantly decreased serum levels of IgE and IL-4, but not IFN-γ, implying the restoration of TMA-induced disruption of Th1/Th2 balance. Quantitative real-time PCR targeting hypervariable regions of 16S rDNA gene of faecal microbiota indicated that the LCR35 treatment increased the population of Bifidobacterium, Lactobacilli, Enterococcus and Bacteroides fragilis group, but decreased those of Clostridium coccoides group.
  
  
  CONCLUSIONS
  LCR35 has the ability to suppress the development of AD in mice, possibly through the modulation of Th1/Th2 balance and gut microbiota.
  
  
  CONCLUSIONS
  LCR35 has a strong potential as a probiotic for preventing AD.
authors.affiliation: Acupuncture and Meridian Science Research Center, College of Korean Medicine, Kyung Hee University, Seoul, Korea.
authors.forename: M
authors.initials: M
authors.lastname: Yeom
authors.affiliation: Acupuncture and Meridian Science Research Center, College of Korean Medicine, Kyung Hee University, Seoul, Korea.
authors.forename: B J
authors.initials: BJ
authors.lastname: Sur
authors.affiliation: Acupuncture and Meridian Science Research Center, College of Korean Medicine, Kyung Hee University, Seoul, Korea.
authors.forename: J
authors.initials: J
authors.lastname: Park
authors.affiliation: Department of Science in Korean Medicine, College of Korean Medicine, Kyung Hee University, Seoul, Korea.
authors.forename: S G
authors.initials: SG
authors.lastname: Cho
authors.affiliation: Acupuncture and Meridian Science Research Center, College of Korean Medicine, Kyung Hee University, Seoul, Korea.
authors.forename: B
authors.initials: B
authors.lastname: Lee
authors.affiliation: Division of Meridian and Structural Medicine, School of Korean Medicine, Pusan National University, Yangsan, Korea.
authors.forename: S T
authors.initials: ST
authors.lastname: Kim
authors.affiliation: Department of Family Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea.
authors.forename: K S
authors.initials: KS
authors.lastname: Kim
authors.affiliation: Acupuncture and Meridian Science Research Center, College of Korean Medicine, Kyung Hee University, Seoul, Korea.|Department of Science in Korean Medicine, College of Korean Medicine, Kyung Hee University, Seoul, Korea.
authors.forename: H
authors.initials: H
authors.lastname: Lee
authors.affiliation: Acupuncture and Meridian Science Research Center, College of Korean Medicine, Kyung Hee University, Seoul, Korea.|Department of Science in Korean Medicine, College of Korean Medicine, Kyung Hee University, Seoul, Korea.
authors.forename: D H
authors.initials: DH
authors.lastname: Hahm
mesh_terms: D000284:Administration, Oral; D000818:Animals; D003876:Dermatitis, Atopic / Q000139:chemically induced / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology; D000069196:Gastrointestinal Microbiome; D006801:Humans; D015847:Interleukin-4 / Q000235:genetics / Q000276:immunology; D052201:Lacticaseibacillus rhamnosus / Q000502:physiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010796:Phthalic Anhydrides / Q000009:adverse effects; D019936:Probiotics / Q000008:administration & dosage*; D060888:Real-Time Polymerase Chain Reaction; D012867:Skin / Q000276:immunology / Q000473:pathology
chemical_list: D010796:Phthalic Anhydrides; D015847:Interleukin-4; C015559:trimellitic anhydride
keywords: Lactobacillus casei variety rhamnosus; Th1/Th2 balance; atopic dermatitis; immunomodulation; microbiota; probiotics