title: Identification of T cell clones without the need for sequencing.
abstract: The brainbow recombination fluorescent protein system has been used for a multitude of applications in fate and lineage tracking. Here, we use a mouse with a ubiquitously expressed brainbow construct, termed the Confetti mouse, to perform T lymphocyte cell lineage tracking. We demonstrate that antigen-specific T lymphocyte clonotypes can be identified and phenotyped using flow cytometry instead of performing expensive and time-consuming methods of single cell sequencing.
authors.affiliation: Department of Microbiology and Immunology, 1510 Clifton Rd NE, Emory University, Atlanta, GA 30322, USA.
authors.forename: Ryan J
authors.initials: RJ
authors.lastname: Martinez
authors.affiliation: Department of Microbiology and Immunology, 1510 Clifton Rd NE, Emory University, Atlanta, GA 30322, USA.
authors.forename: Dennis K
authors.initials: DK
authors.lastname: Neeld
authors.affiliation: Department of Microbiology and Immunology, 1510 Clifton Rd NE, Emory University, Atlanta, GA 30322, USA. Electronic address: bevavol@emory.edu.
authors.forename: Brian D
authors.initials: BD
authors.lastname: Evavold
mesh_terms: D000818:Animals; D002999:Clone Cells; D005434:Flow Cytometry / Q000379:methods; D015870:Gene Expression; D017930:Genes, Reporter; D051379:Mice; D008822:Mice, Transgenic; D011948:Receptors, Antigen, T-Cell / Q000235:genetics; D017422:Sequence Analysis, DNA; D059010:Single-Cell Analysis / Q000379:methods*; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D013629:Tamoxifen / Q000008:administration & dosage / Q000494:pharmacology
chemical_list: D011948:Receptors, Antigen, T-Cell; D013629:Tamoxifen
keywords: Clonotype; Lineage tracking; T cell receptor