title: Antibacterial releasing titanium surface using albumin nanoparticle carriers.
abstract: We developed a simple and highly efficient method for delivery from titanium (Ti) surfaces using albumin nanoparticle carriers. A Ti disc with a resorbable blasting media surface was used as a metal implant with a localized drug delivery structure. Human serum albumin (HSA) nanoparticles loaded with chlorhexidine (CHX) diacetate salt hydrate as the model drug were fabricated using a desolvation technique. The CHX-loaded HSA nanoparticles produced were cross linked with glutaraldehyde (GA). The nanoparticles were pre-coated with positively-charged polyethylenimine (PEI) molecules and then immobilized via electrical interactions on the negatively charged Ti disc surface. Our results suggested that the PEI-coated HSA nanoparticles loaded with CHX (PEI-CHX-HSA) were incorporated successfully and well-dispersed on the Ti disc surfaces. The agar diffusion test on the Ti surface treated with PEI-CHX-HSA nanoparticles showed a larger growth inhibition zone of Streptococcus mutans versus the control Ti surface, suggesting that this innovative delivery platform imparts potent antibacterial activity to the Ti surface. Thus, CHX, which inhibits the growth of oral bacteria, can be efficiently incorporated onto Ti surfaces by using HSA nanoparticles.
authors.forename: Da Hye
authors.initials: DH
authors.lastname: Kim
authors.forename: Kyo-Han
authors.initials: KH
authors.lastname: Kim
authors.forename: Tae-Yub
authors.initials: TY
authors.lastname: Kwon
authors.forename: Seok Hwa
authors.initials: SH
authors.lastname: Choi
authors.forename: Seong Soo
authors.initials: SS
authors.lastname: Kang
authors.forename: Soon-Taek
authors.initials: ST
authors.lastname: Kwon
authors.forename: Dae-Hyun
authors.initials: DH
authors.lastname: Cho
authors.forename: Hee Dong
authors.initials: HD
authors.lastname: Kim
authors.forename: Jun Sik
authors.initials: JS
authors.lastname: Son
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry* / Q000493:pharmacokinetics; D002710:Chlorhexidine / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D016503:Drug Delivery Systems / Q000379:methods; D006801:Humans; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D012709:Serum Albumin / Q000008:administration & dosage / Q000737:chemistry*; D013295:Streptococcus mutans / Q000187:drug effects; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D012709:Serum Albumin; D014025:Titanium; D002710:Chlorhexidine