title: Multicolor FISHs for simultaneous detection of genes and DNA segments on human chromosomes.
abstract: We have developed a convenient multicolor fluorescent in situ hybridization (FISH) (five-, four-, three-, and two-color FISHs) for detecting specific genes/DNA segments on the human chromosomes. As a foundation of multicolor FISH, we first isolated 80 bacterial artificial chromosome (BAC) probes that specifically detect the peri-centromeres (peri-CEN) and subtelomeres (subTEL) of 24 different human chromosomes (nos. 1~22, X, and Y) by screening our homemade BAC library (Keio BAC library) consisting of 200,000 clones. Five-color FISH was performed using human DNA segments specific for peri-CEN or subTEL, which were labeled with five different fluorescent dyes [7-diethylaminocoumarin (DEAC): blue, fluorescein isothiocyanate (FITC): green, rhodamine 6G (R6G): yellow, TexRed: red, and cyanine5 (Cy5): purple]. To observe FISH signals under a fluorescence microscope, five optic filters were carefully chosen to avoid overlapping fluorescence emission. Five-color FISH and four-color FISH enabled us to accurately examine the numerical anomaly of human chromosomes. Three-color FISH using two specific BAC clones, that distinguish 5' half of oncogene epidermal growth factor receptor (EGFR) from its 3' half, revealed the amplification and truncation of EGFR in EGFR-overproducing cancer cells. Moreover, two-color FISH readily detected a fusion gene in leukemia cells such as breakpoint cluster region (BCR)/Abelson murine leukemia viral oncogene homologue (ABL) on the Philadelphia (Ph') chromosome with interchromosomal translocation. Some other successful cases such as trisomy 21 of Down syndrome are presented. Potential applications of multicolor FISH will be discussed.
authors.affiliation: Keio University, Tsukuba, Ibaraki, Japan. smzgsp@dmb.med.keio.ac.jp.
authors.forename: Nobuyoshi
authors.initials: N
authors.lastname: Shimizu
authors.affiliation: Keio University, Tsukuba, Ibaraki, Japan.
authors.forename: Masahiko
authors.initials: M
authors.lastname: Maekawa
authors.affiliation: Keio University, Tsukuba, Ibaraki, Japan.
authors.forename: Satoko
authors.initials: S
authors.lastname: Asai
authors.affiliation: Keio University, Tsukuba, Ibaraki, Japan.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Shimizu
mesh_terms: D002869:Chromosome Aberrations; D025063:Chromosome Disorders / Q000175:diagnosis / Q000235:genetics; D022202:Chromosomes, Artificial, Bacterial; D002877:Chromosomes, Human; D015342:DNA Probes; D015698:Genomic Library; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D036641:Microscopy, Fluorescence, Multiphoton; D013194:Staining and Labeling
chemical_list: D015342:DNA Probes
keywords: BAC clones; fusion genes; multicolor FISH; numerical anomaly; trisomy 21