title: Bacillus phytases: Current status and future prospects.
abstract: Phytases catalyze the hydrolysis of phytic acid in a stepwise manner to lower inositol phosphates, myo-inositol (having important role in metabolism and signal transduction pathways), and inorganic phosphate. These enzymes have been widely used in animal feed in order to improve phosphorus nutrition and to decrease pollution in animal waste. Compared to previously described phytases, the phytase (PhyL) from Bacillus licheniformis ATCC 14580 has attractive biochemical properties which can increase the profitability of several biotechnological procedures (animal nutrition, humain health…etc). Due to its amino acid sequence with critical substitutions, the PhyL could be a model to enhance other phytases features, in terms of thermal stability and high activity. Otherwise, an engineered PhyL, with low pH optimum, will represent a challenge within the class of β- propeller phytases.
authors.affiliation: a Faculty of Sciences of Gafsa - Unit of Macromolecular Biochemistry and Genetic ; Department of Life Sciences ; Zarroug, Gafsa, Tunisia.
authors.forename: Mohamed Ali
authors.initials: MA
authors.lastname: Borgi
authors.forename: Samira
authors.initials: S
authors.lastname: Boudebbouze
authors.forename: Héla
authors.initials: H
authors.lastname: Mkaouar
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Maguin
authors.forename: Moez
authors.initials: M
authors.lastname: Rhimi
mesh_terms: D010832:6-Phytase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001407:Bacillus / Q000737:chemistry / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001709:Biotechnology / Q000379:methods; D004795:Enzyme Stability; D015870:Gene Expression; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D015202:Protein Engineering; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D010832:6-Phytase
keywords: Bacillus licheniformis; biotechnological applications; enzyme engineering; phytase; protein biochemistry