title: Effects of Trehalose on Thermodynamic Properties of Alpha-synuclein Revealed through Synchrotron Radiation Circular Dichroism.
abstract: Many neurodegenerative diseases, including Huntington's, Alzheimer's and Parkinson's diseases, are characterized by protein misfolding and aggregation. The capability of trehalose to interfere with protein misfolding and aggregation has been recently evaluated by several research groups. In the present work, we studied, by means of synchrotron radiation circular dichroism (SRCD) spectroscopy, the dose-effect of trehalose on α-synuclein conformation and/or stability to probe the capability of this osmolyte to interfere with α-synuclein's aggregation. Our study indicated that a low trehalose concentration stabilized α-synuclein folding much better than at high concentration by blocking in vitro α-synuclein's polymerisation. These results suggested that trehalose could be associated with other drugs leading to a new approach for treating Parkinson's and other brain-related diseases.
authors.affiliation: Institute of Biomolecular Chemistry of CNR, Padua Unit, Padua 35131, Italy. paolo.ruzza@cnr.it.
authors.forename: Paolo
authors.initials: P
authors.lastname: Ruzza
authors.affiliation: Diamond Light Source Ltd., Harwell Innovation Campus, Chilton, Didcot, Oxfordshire OX11 0QX, UK. rohanah.hussain@diamond.ac.uk.
authors.forename: Rohanah
authors.initials: R
authors.lastname: Hussain
authors.affiliation: Institute of Biomolecular Chemistry of CNR, Padua Unit, Padua 35131, Italy. barbara.biondi@cnr.it.
authors.forename: Barbara
authors.initials: B
authors.lastname: Biondi
authors.affiliation: Institute of Biomolecular Chemistry of CNR, Padua Unit, Padua 35131, Italy. andrea.calderan@icb.cnr.it.
authors.forename: Andrea
authors.initials: A
authors.lastname: Calderan
authors.affiliation: Department of Biology, University of Padua, Padua 35122, Italy. isabella.tessari@unipd.it.
authors.forename: Isabella
authors.initials: I
authors.lastname: Tessari
authors.affiliation: Department of Biology, University of Padua, Padua 35122, Italy. luigi.bubacco@unipd.it.
authors.forename: Luigi
authors.initials: L
authors.lastname: Bubacco
authors.affiliation: Diamond Light Source Ltd., Harwell Innovation Campus, Chilton, Didcot, Oxfordshire OX11 0QX, UK. giuliano.siligardi@diamond.ac.uk.
authors.forename: Giuliano
authors.initials: G
authors.lastname: Siligardi
mesh_terms: D002942:Circular Dichroism; D009994:Osmolar Concentration; D011487:Protein Conformation; D013816:Thermodynamics; D014199:Trehalose / Q000737:chemistry*; D051844:alpha-Synuclein / Q000737:chemistry*
chemical_list: D051844:alpha-Synuclein; D014199:Trehalose