title: Higher SOD1 Gene Expression in Cumulus Cells From Infertile Women With Moderate and Severe Endometriosis.
abstract: It is questioned whether worsening of oocyte quality and oxidative stress (OS) are involved in the pathogenesis of infertility related to endometriosis and in compromised intracytoplasmic sperm injection (ICSI) outcomes. Cumulus cells (CCs) protect oocytes from entering apoptosis induced by OS. Thus, we carried out a case-control study comparing expression of superoxide dismutase 1 (SOD1), superoxide dismutase 2 (SOD2), and glutathione peroxidase 4 (GPX4; genes encoding for the main antioxidant enzymes) in CCs from mature oocytes of 26 infertile patients with minimal/mild endometriosis, 14 patients with moderate/severe endometriosis, and 41 controls undergoing controlled ovarian stimulation for ICSI, using real-time polymerase chain reaction. As a secondary objective, we investigated the interaction between the expression of these genes and clinical pregnancy (CP) by a statistical model. Only infertile women with moderate/severe endometriosis showed increased expression of the SOD1 in CCs compared to women with minimal/mild endometriosis and controls, with a positive interaction between increased expression and the occurrence of CP, suggesting that SOD1 might be a potential biomarker of CP following ICSI.
authors.affiliation: Laboratory of the Biology of Reproduction, Department of Gynecology and Obstetrics, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Flávia Cappello
authors.initials: FC
authors.lastname: Donabela
authors.affiliation: Laboratory of the Biology of Reproduction, Department of Gynecology and Obstetrics, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Juliana
authors.initials: J
authors.lastname: Meola
authors.affiliation: Laboratory of the Biology of Reproduction, Department of Gynecology and Obstetrics, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Cristiana Carolina
authors.initials: CC
authors.lastname: Padovan
authors.affiliation: Laboratory of the Genetics, Department of Genetics, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Cláudia Cristina Paro
authors.initials: CC
authors.lastname: de Paz
authors.affiliation: Laboratory of the Biology of Reproduction, Department of Gynecology and Obstetrics, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, São Paulo, Brazil drapaulanavarro@gmail.com.
authors.forename: Paula Andrea
authors.initials: PA
authors.lastname: Navarro
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D054885:Cumulus Cells / Q000201:enzymology*; D004715:Endometriosis / Q000150:complications* / Q000175:diagnosis; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000175:diagnosis / Q000201:enzymology / Q000209:etiology* / Q000235:genetics / Q000628:therapy; D011446:Prospective Studies; D060888:Real-Time Polymerase Chain Reaction; D027724:Reproductive Techniques, Assisted; D012720:Severity of Illness Index; D013482:Superoxide Dismutase / Q000235:genetics*; D000072105:Superoxide Dismutase-1; D015854:Up-Regulation
chemical_list: C000606290:SOD1 protein, human; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1
keywords: ICSI; SOD1; cumulus cells; endometriosis; infertility