title: Fluorescent Visualisation of Oxytocin in the Hypothalamo-neurohypophysial/-spinal Pathways After Chronic Inflammation in Oxytocin-Monomeric Red Fluorescent Protein 1 Transgenic Rats.
abstract: Oxytocin (OXT) is a well-known neurohypophysial hormone that is synthesised in the paraventricular (PVN) and supraoptic nuclei (SON) of the hypothalamus. The projection of magnocellular neurosecretory cells, which synthesise OXT and arginine vasopressin in the PVN and SON, to the posterior pituitary plays an essential role in mammalian labour and lactation through its peripheral action. However, previous studies have shown that parvocellular OXTergic cells in the PVN, which project to the medulla and spinal cord, are involved in various physiological functions (e.g. sensory modulation and autonomic). In the present study, we examined OXT expression in the PVN, SON and spinal cord after chronic inflammation from adjuvant arthritis (AA). We used transgenic rats that express OXT and the monomeric red fluorescent protein 1 (mRFP1) fusion gene to visualise both the magnocellular and parvocellular OXTergic pathways. OXT-mRFP1 fluorescence intensity was significantly increased in the PVN, SON, dorsal horn of the spinal cord and posterior pituitary in AA rats. The levels of OXT-mRFP1 mRNA were significantly increased in the PVN and SON of AA rats. These results suggested that OXT was up-regulated in both hypothalamic magnocellular neurosecretory cells and parvocellular cells by chronic inflammation, and also that OXT in the PVN-spinal pathway may be involved in sensory modulation. OXT-mRFP1 transgenic rats are a very useful model for visualising the OXTergic pathways from vesicles in a single cell to terminals in in vitro preparations.
authors.affiliation: Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.|Department of Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Matsuura
authors.affiliation: Department of Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kawasaki
authors.affiliation: Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Hashimoto
authors.affiliation: Department of Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ishikura
authors.affiliation: Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Yoshimura
authors.affiliation: Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
authors.forename: J-I
authors.initials: JI
authors.lastname: Ohkubo
authors.affiliation: Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Maruyama
authors.affiliation: Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.|Department of Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Motojima
authors.affiliation: Department of Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Sabanai
authors.affiliation: Department of Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Mori
authors.affiliation: Department of Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ohnishi
authors.affiliation: Department of Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Sakai
authors.affiliation: Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Ueta
mesh_terms: D000818:Animals; D001168:Arthritis / Q000378:metabolism*; D002908:Chronic Disease; D004195:Disease Models, Animal; D007249:Inflammation / Q000378:metabolism*; D049408:Luminescent Agents; D008164:Luminescent Proteins / Q000235:genetics; D008297:Male; D010121:Oxytocin / Q000235:genetics / Q000378:metabolism*; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism*; D051381:Rats; D055647:Rats, Transgenic; D017208:Rats, Wistar; D015398:Signal Transduction / Q000502:physiology*; D013116:Spinal Cord / Q000378:metabolism*; D013495:Supraoptic Nucleus / Q000378:metabolism*; D000097573:Red Fluorescent Protein
chemical_list: D049408:Luminescent Agents; D008164:Luminescent Proteins; D010121:Oxytocin; D000097573:Red Fluorescent Protein
keywords: arthritic rats; chronic inflammation; fluorescent protein; oxytocin