title: [Serological tests of the truncated TTSuV2 ORF1 recombinant proteins with the porcine sera].
abstract: OBJECTIVE
  We studied the serological response between the special regions on the Torque teno sus virus 2 (TTSuV2) ORF1 coded protein and the porcine sera from conventional pigs.
  
  
  METHODS
  Based on a Chinese TTSuV2 strain from Guangdong province, two overlapped virus proteins were expressed from Escherichia coli. Then, purified recombinant TTSuV2 ORF1a and TTSuV2 ORF1ab proteins were used as the antigens in the Western Blotting and ELISA assay.
  
  
  RESULTS
  The recombinant TTSuV2 ORF1a and TTSuV2 ORF1ab proteins were identified with the special tag monoclonal antibody. The results of the ELISA tests shown that there were significant relationships between two groups of dates from the recombinant TTSuV2 ORF1a and TTSuV2 ORF1ab proteins antigenic assay. The results of the following Western Blotting assay indicated that the TTSuV2-specific IgG antibodies were contained in pig sera.
  
  
  CONCLUSIONS
  The truncated TTSuV2 ORF1a protein (positions 168 to 346 corresponding to TTSuV2 GDIMA1) contains important B cell epitopes which can stimulate immune system antibody secretion. The truncated TTSuV2 ORF1a protein could be effective in TTSuV2 immunodiagnosis.
authors.forename: Zhongsheng
authors.initials: Z
authors.lastname: Li
authors.forename: Yiwen
authors.initials: Y
authors.lastname: Chen
authors.forename: Aiquan
authors.initials: A
authors.lastname: Bai
authors.forename: Shanzhen
authors.initials: S
authors.lastname: Chen
authors.forename: Lulu
authors.initials: L
authors.lastname: Wang
authors.forename: Huochun
authors.initials: H
authors.lastname: Yao
authors.forename: Guiping
authors.initials: G
authors.lastname: Wang
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D004266:DNA Virus Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D016366:Open Reading Frames; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000821:virology; D022783:Torque teno virus / Q000235:genetics / Q000276:immunology*; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D011994:Recombinant Proteins; D014764:Viral Proteins