title: Chemical Constituents and Evaluation of Antimicrobial and Cytotoxic Activities of Kielmeyera coriacea Mart. & Zucc. Essential Oils.
abstract: Many essential oils (EOs) of different plant species possess interesting antimicrobial effects on buccal microorganisms and cytotoxic properties. EOs of Kielmeyera coriacea Mart. & Zucc. were analyzed by gas chromatography coupled to mass spectrometry (GC-MS). The EO from leaves is rich in sesquiterpenes hydrocarbons and oxygenated sesquiterpenes. The three major compounds identified were germacrene-D (24.2%), (E)-caryophyllene (15.5%), and bicyclogermacrene (11.6%). The inner bark EO is composed mainly of sesquiterpenes hydrocarbons and the major components are alpha-copaene (14.9%) and alpha-(E)-bergamotene (13.0%). The outer bark EO is composed mainly of oxygenated sesquiterpenes and long-chain alkanes, and the major components are alpha-eudesmol (4.2%) and nonacosane (5.8%). The wood EO is mainly composed of long-chain alkanes and fatty acids, and the major components are nonacosane (9.7%) and palmitic acid (16.2%). The inner bark EO showed the strongest antimicrobial activity against the anaerobic bacteria Prevotella nigrescens (minimum inhibitory concentration-MIC of 50 µg mL(-1)). The outer bark and wood EOs showed MICs of 100 µg mL(-1) for all aerobic microorganisms tested. The EOs presented low toxicity to Vero cells. These results suggest that K. coriacea, a Brazilian plant, provide initial evidence of a new and alternative source of substances with medicinal interest.
authors.affiliation: Nucleus of Research in Natural Products, Chemistry Institute, Federal University of Uberlândia, 38400-902 Uberlândia, MG, Brazil ; Goian Federal Institute, Campus Morrinhos, 75650-000 Morrinhos, GO, Brazil.
authors.forename: Carla de M
authors.initials: Cde M
authors.lastname: Martins
authors.affiliation: Nucleus of Research in Natural Products, Chemistry Institute, Federal University of Uberlândia, 38400-902 Uberlândia, MG, Brazil.
authors.forename: Evandro A
authors.initials: EA
authors.lastname: do Nascimento
authors.affiliation: Nucleus of Research in Natural Products, Chemistry Institute, Federal University of Uberlândia, 38400-902 Uberlândia, MG, Brazil.
authors.forename: Sérgio A L
authors.initials: SA
authors.lastname: de Morais
authors.affiliation: Nucleus of Research in Natural Products, Chemistry Institute, Federal University of Uberlândia, 38400-902 Uberlândia, MG, Brazil.
authors.forename: Alberto
authors.initials: A
authors.lastname: de Oliveira
authors.affiliation: Nucleus of Research in Natural Products, Chemistry Institute, Federal University of Uberlândia, 38400-902 Uberlândia, MG, Brazil.
authors.forename: Roberto
authors.initials: R
authors.lastname: Chang
authors.affiliation: Nucleus of Research in Natural Products, Chemistry Institute, Federal University of Uberlândia, 38400-902 Uberlândia, MG, Brazil.
authors.forename: Luís C S
authors.initials: LC
authors.lastname: Cunha
authors.affiliation: Nucleus of Research in Natural Products, Chemistry Institute, Federal University of Uberlândia, 38400-902 Uberlândia, MG, Brazil.
authors.forename: Mário M
authors.initials: MM
authors.lastname: Martins
authors.affiliation: Nucleus of Research in Sciences and Technology, Laboratory of Research in Applied Microbiology, University of Franca, 14404-600 Franca, SP, Brazil.
authors.forename: Carlos Henrique G
authors.initials: CH
authors.lastname: Martins
authors.affiliation: Nucleus of Research in Sciences and Technology, Laboratory of Research in Applied Microbiology, University of Franca, 14404-600 Franca, SP, Brazil.
authors.forename: Thaís da S
authors.initials: Tda S
authors.lastname: Moraes
authors.affiliation: Institute of Biomedical Sciences, Laboratory of Trypanosomatids, Federal University of Uberlândia, 38400-902 Uberlândia, MG, Brazil.
authors.forename: Paulla V
authors.initials: PV
authors.lastname: Rodrigues
authors.affiliation: Institute of Biomedical Sciences, Laboratory of Trypanosomatids, Federal University of Uberlândia, 38400-902 Uberlândia, MG, Brazil.
authors.forename: Cláudio V
authors.initials: CV
authors.lastname: da Silva
authors.affiliation: Nucleus of Research in Natural Products, Chemistry Institute, Federal University of Uberlândia, 38400-902 Uberlândia, MG, Brazil.
authors.forename: Francisco J T
authors.initials: FJ
authors.lastname: de Aquino