title: Dominance of Giardia duodenalis assemblage A and Enterocytozoon bieneusi genotype BEB6 in sheep in Inner Mongolia, China.
abstract: To examine the occurrence and genotype distribution of Giardia duodenalis and Enterocytozoon bieneusi in sheep, fecal specimens were collected from 162 lambs and 213 ewes on seven farms in the Hulunbeier Prairie in Inner Mongolia, China. By PCR analysis of the triose-phosphate isomerase gene, 16 of the 375 (4.3%) specimens were positive for G. duodenalis, with 13 sequenced successfully belonging to assemblage A. Lambs had a significantly higher infection rate than ewes (8.6% versus 0.9%, respectively). The dominance of assemblage A in sheep was supported by PCR analysis of the β-giardin and glutamate dehydrogenase genes; 4 of 7 β-giardin-positive specimens and 3 of 5 glutamate dehydrogenase-positive specimens were identified as assemblage A. A much higher occurrence of E. bieneusi was detected by PCR analysis of the ribosomal internal transcribed spacer; 77.8% of lambs and 62.9% of ewes were positive for the pathogen. Two genotypes of E. bieneusi were found: BEB6 and CM7. Genotype BEB6 was seen in 237 animals and on all seven farms, whereas genotype CM7 was detected in 23 animals from six farms. These data indicate that sheep in Inner Mongolia are commonly infected with G. duodenalis assemblage A and E. bieneusi genotype BEB6, two zoonotic pathogens in China.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai, 200237, China.
authors.forename: Jianbin
authors.initials: J
authors.lastname: Ye
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia, 30329, USA.
authors.forename: Lihua
authors.initials: L
authors.lastname: Xiao
authors.affiliation: State Key Laboratory of Bioreactor Engineering, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai, 200237, China.
authors.forename: Yuanfei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Bioreactor Engineering, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai, 200237, China; Centers for Disease Control and Prevention, Atlanta, Georgia, 30329, USA.
authors.forename: Yaqiong
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia, 30329, USA.
authors.forename: Dawn M
authors.initials: DM
authors.lastname: Roellig
authors.affiliation: State Key Laboratory of Bioreactor Engineering, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai, 200237, China. Electronic address: yyfeng@ecust.edu.cn.
authors.forename: Yaoyu
authors.initials: Y
authors.lastname: Feng
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D002681:China / Q000453:epidemiology; D021862:Enterocytozoon / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D005838:Genotype; D016829:Giardia lamblia / Q000235:genetics / Q000302:isolation & purification*; D005873:Giardiasis / Q000453:epidemiology / Q000662:veterinary*; D006801:Humans; D016881:Microsporidiosis / Q000453:epidemiology / Q000662:veterinary*; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000662:veterinary; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology / Q000469:parasitology*; D014305:Triose-Phosphate Isomerase / Q000235:genetics; D015047:Zoonoses
chemical_list: D014305:Triose-Phosphate Isomerase
keywords: Enterocytozoon bieneusi; Genotype; Giardia duodenalis; Molecular epidemiology; Sheep; Zoonosis