title: Chemotherapeutic Drugs Interfere with Gene Delivery Mediated by Chitosan-Graft-Poly(ethylenimine).
abstract: Combined chemo-gene therapy is one of the treatment modalities that have attracted extensive research interests; however, there is little information regarding the influence of drug application on gene transfer. This study bridges this gap by examining how chemotherapeutic drugs (teniposide, cis-diamminedichloroplatinum(II) and temozolomide) interfere with polyplex formation and transfection of chitosan-graft-poly(ethylenimine). Our results indicate that the degree of drug interference varies with the mechanism of drug action, with the transgene expression being severely suppressed when the plasmid is co-delivered with cis-diamminedichloroplatinum(II) or teniposide but not temozolomide. In addition, the interference with transfection by drugs varies with different gene/drug co-formulations. This is the first study to evidence that, though combined chemo-gene therapy has therapeutic potential, some chemotherapeutic drugs may reduce the treatment efficiency of gene therapy.
authors.affiliation: School of Medicine, Shenzhen University, Shenzhen, China; Department of Mechanical Engineering, The University of Hong Kong, Hong Kong Special Administrative Region, China.
authors.forename: Wing-Fu
authors.initials: WF
authors.lastname: Lai
authors.affiliation: School of Medicine, Shenzhen University, Shenzhen, China.
authors.forename: Marie C
authors.initials: MC
authors.lastname: Lin
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000633:toxicity; D045744:Cell Line, Tumor; D002626:Chemistry, Pharmaceutical; D048271:Chitosan / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry; D004347:Drug Interactions; D015870:Gene Expression; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D015316:Genetic Therapy; D006801:Humans; D011094:Polyethyleneimine / Q000737:chemistry; D014162:Transfection
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D011094:Polyethyleneimine; D048271:Chitosan