title: Stimulation of cutaneous wound healing by an FPR2-specific peptide agonist WKYMVm.
abstract: Diabetes is one of the most common human diseases and 15% of the 200 million diabetics worldwide suffer from diabetic wounds. Development of new therapeutic agents is needed for treatment of diabetic wounds. Wound healing is mediated by multiple steps, including inflammation, epithelialization, neoangiogenesis, and granulation. Formyl peptide receptor 2 has been known to stimulate angiogenesis, which is essential for tissue repair and cutaneous wound healing. In this study, we explored the therapeutic effects of WKYMVm (Trp-Lys-Tyr-Met-Val-D-Met-NH2), a synthetic peptide agonist of formyl peptide receptor 2, on cutaneous wounds in streptozotocin-induced diabetic rats. Topical application of WKYMVm onto cutaneous wounds stimulated formation of von Willebrand factor-positive capillary and α-smooth muscle actin-positive arteriole with a maximal stimulation on day 6, suggesting WKYMVm-stimulated angiogenesis. Infiltration of immune cells could be detected on early phase during wound healing and WKYMVm treatment acutely augmented infiltration of CD68-positive macrophages. In addition, reepithelialization and granulation tissue formation were accelerated by treatment with WKYMVm. These results suggest that WKYMVm has therapeutic effects on diabetic wounds by stimulating angiogenesis and infiltration of immune cells.
authors.affiliation: Department of Physiology.
authors.forename: Yang Woo
authors.initials: YW
authors.lastname: Kwon
authors.affiliation: Department of Physiology.
authors.forename: Soon Chul
authors.initials: SC
authors.lastname: Heo
authors.affiliation: Department of Physiology.
authors.forename: Il Ho
authors.initials: IH
authors.lastname: Jang
authors.affiliation: Department of Physiology.
authors.forename: Geun Ok
authors.initials: GO
authors.lastname: Jeong
authors.affiliation: Department of Physiology.
authors.forename: Jung Won
authors.initials: JW
authors.lastname: Yoon
authors.affiliation: Department of Dermatology, School of Medicine, Pusan National University, Yangsan, Gyeongsangnam-do, Republic of Korea.
authors.forename: Je-Ho
authors.initials: JH
authors.lastname: Mun
authors.affiliation: Department of Physiology.|Research Institute of Convergence Biomedical Science and Technology, Pusan National University Yangsan Hospital, Yangsan, Gyeongsangnam-do, Republic of Korea.
authors.forename: Jae Ho
authors.initials: JH
authors.lastname: Kim
mesh_terms: D000287:Administration, Topical; D000818:Animals; D002630:Chemotactic Factors; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000188:drug therapy* / Q000378:metabolism; D008297:Male; D018919:Neovascularization, Physiologic / Q000187:drug effects; D009842:Oligopeptides / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D044042:Receptors, Formyl Peptide / Q000819:agonists*; D012867:Skin / Q000098:blood supply / Q000187:drug effects / Q000473:pathology; D012883:Skin Ulcer / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D002630:Chemotactic Factors; D009842:Oligopeptides; D044042:Receptors, Formyl Peptide; C113617:Trp-Lys-Tyr-Met-Val-Met