title: Nanocrystal-based electrochemiluminescence sensor for cell detection with Au nanoparticles and isothermal circular double-assisted signal amplification.
abstract: Here we have developed a sensitive cancer cell amplified detection method which combined Au NPs enhanced electrochemiluminescence (ECL) of CdS nanocrystals (NCs) film, with isothermal circular amplification reaction of polymerase. In DNA circular amplification detection system, hairpin DNA beacon/Au NPs composite modified CdS NCs film was used as an ECL emitter. Messenger DNA is hybridized with the aptamer modified on magnetic beads (MBs) to form MB-Au bioconjugates. In the presence of HL-60 cell, the aptamer would conjugate with the glycoprotein at cell surface and messenger DNA sequence would be released. The released messenger DNA sequence was then introduced into the cycle amplification system to trigger circular polymerizations. This assay allows us to determine the released messenger DNA equivalent to 10 cells and exhibits a significant specificity for HL-60 cells.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
authors.forename: Pan-Pan
authors.initials: PP
authors.lastname: Dai
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
authors.forename: Jin-Yi
authors.initials: JY
authors.lastname: Li
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China. Electronic address: xujj@nju.edu.cn.
authors.forename: Jing-Juan
authors.initials: JJ
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China. Electronic address: hychen@nju.edu.cn.
authors.forename: Hong-Yuan
authors.initials: HY
authors.lastname: Chen
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D019187:Cadmium Compounds / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D018922:HL-60 Cells; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods; D008280:Magnetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D021141:Nucleic Acid Amplification Techniques; D012333:RNA, Messenger / Q000032:analysis* / Q000737:chemistry; D013440:Sulfides / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D019187:Cadmium Compounds; D012333:RNA, Messenger; D013440:Sulfides; C034939:cadmium sulfide; D006046:Gold
keywords: Au nanoparticles; CdS nanocrystals; Electrochemiluminescence; Energy transfer; HL-60 cancer cells; Isothermal circular amplification