title: 2,3,5-Trimethoxy-4-cresol, an anti-metastatic constituent from the solid-state cultured mycelium of Antrodia cinnamomea and its mechanism.
abstract: Antrodia cinnamomea is a valuable and unique edible fungus originating from the forests of Taiwan. In this study, an anti-metastatic compound, 2,3,5-trimethoxy-4-cresol (TMC), was isolated from the solid-state cultured mycelium of A. cinnamomea. According to the results obtained from cell wound healing, cell migration and invasion assays, TMC effectively suppressed movement, migration and invasion of lung cancer cells at the dosage of 5-40 μM, which was non-toxic to A549 cells. In addition, TMC reduced protein expression of Akt, MMP-2 and MMP-9 and enhanced E-cadherin and TIMP-1 protein expression, which are known to regulate cell adhesion, migration and invasion. Taken together, TMC effectively suppresses movement, migration and invasion of lung cancer cells, and achieves an anti-cancer metastasis effect.
authors.affiliation: Taiwan Leader Biotech Company, Taipei, Taiwan.
authors.forename: Chin-Chung
authors.initials: CC
authors.lastname: Lin
authors.forename: Ching-Chun
authors.initials: CC
authors.lastname: Chen
authors.forename: Yueh-Hsiung
authors.initials: YH
authors.lastname: Kuo
authors.forename: Jong-Tar
authors.initials: JT
authors.lastname: Kuo
authors.forename: K J
authors.initials: KJ
authors.lastname: Senthil Kumar
authors.forename: Sheng-Yang
authors.initials: SY
authors.lastname: Wang
mesh_terms: D055491:Antrodia / Q000737:chemistry*; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy* / Q000401:mortality; D058750:Epithelial-Mesenchymal Transition; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000401:mortality; D025282:Mycelium / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*