title: pH-responsive scaffolds generate a pro-healing response.
abstract: A principal challenge in wound healing is a lack of cell recruitment, cell infiltration, and vascularization, which occurs in the absence of temporal and spatial cues. We hypothesized that a scaffold that expands due to local changes in pH may alter oxygen and nutrient transport and the local cell density, leading to enhanced cell deposition and survival. In this study, we present a pH-responsive scaffold that increases oxygen transport, as confirmed by our finite element model analysis, and cell proliferation relative to a non-responsive scaffold. In vivo, responsive scaffolds induce a pro-healing gene expression profile indicative of enhanced angiogenesis, granulation tissue formation, and tissue remodeling. Scaffolds that stretch in response to their environment may be a hallmark for tissue regeneration.
authors.affiliation: School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA.
authors.forename: Jin-Oh
authors.initials: JO
authors.lastname: You
authors.affiliation: School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA.
authors.forename: Marjan
authors.initials: M
authors.lastname: Rafat
authors.affiliation: School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA.
authors.forename: Dariela
authors.initials: D
authors.lastname: Almeda
authors.affiliation: Department of Biomedical Engineering, City College of New York, New York, NY 10031, USA.
authors.forename: Natalia
authors.initials: N
authors.lastname: Maldonado
authors.affiliation: School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA.
authors.forename: Peng
authors.initials: P
authors.lastname: Guo
authors.affiliation: Division of Vascular and Endovascular Surgery, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA.
authors.forename: Christoph S
authors.initials: CS
authors.lastname: Nabzdyk
authors.affiliation: Division of Vascular and Endovascular Surgery, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA.
authors.forename: Maggie
authors.initials: M
authors.lastname: Chun
authors.affiliation: Division of Vascular and Endovascular Surgery, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA.
authors.forename: Frank W
authors.initials: FW
authors.lastname: LoGerfo
authors.affiliation: School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA.
authors.forename: John W
authors.initials: JW
authors.lastname: Hutchinson
authors.affiliation: Division of Vascular and Endovascular Surgery, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA.
authors.forename: Leena K
authors.initials: LK
authors.lastname: Pradhan-Nabzdyk
authors.affiliation: School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA; Vascular Biology Program, Boston Children's Hospital, Boston, MA 02115, USA. Electronic address: dauguste@ccny.cuny.edu.
authors.forename: Debra T
authors.initials: DT
authors.lastname: Auguste
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002470:Cell Survival; D020342:Finite Element Analysis; D006863:Hydrogen-Ion Concentration; D008689:Methacrylates / Q000737:chemistry*; D051379:Mice; D041681:NIH 3T3 Cells; D010100:Oxygen / Q000378:metabolism*; D016062:Porosity; D017208:Rats, Wistar; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*; D014945:Wound Healing
chemical_list: D008689:Methacrylates; C005044:hydroxyethyl methacrylate; C049840:2-(dimethylamino)ethyl methacrylate; D010100:Oxygen
keywords: Cell infiltration; DMAEMA; Oxygen transport; Tissue engineering; pH-responsive