title: A novel analysis method for lactate dehydrogenase activity in serum samples based on fluorescence capillary analysis.
abstract: Based on fluorescence capillary analysis technology, a method for quantitating lactate dehydrogenase (LDH) activity in a micro-volume sample was developed. Sample and reagent consumptions were merely 2 and 16 μL per time, respectively. The optimized test conditions were as follows. The reaction reagent consisted of 0.10 M phosphate buffer (pH 6.5), 0.30 mM NADH and 1.20 mM pyruvate. NADH standard was prepared with a phosphate buffer of pH 8.0, and its linear response was controlled in 0.05 - 0.30 mM. LDH standards containing 2.0 mM PEG could exhibit long-term stability. Under the optimized conditions, a linear response for LDH from 50 to 1200 U L(-1) and a detection limit of 31 U L(-1) were obtained with good precision (RSD: 2.1 - 2.2%, n = 10) and better recovery of 96 - 105%. The method's characteristics was high sensitivity, low consumptions, simple operations, good precision and reliability, lending itself to the miniaturization of fluorophotometer which transformed into a bedside instrument in the hospital.
authors.affiliation: School of Chemical Engineering, Sichuan University.
authors.forename: Qiao-Jing
authors.initials: QJ
authors.lastname: Li
authors.forename: Yong-Sheng
authors.initials: YS
authors.lastname: Li
authors.forename: Xiu-Feng
authors.initials: XF
authors.lastname: Gao
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D004789:Enzyme Activation; D015590:Fluorophotometry / Q000379:methods*; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood* / Q000378:metabolism
chemical_list: D007770:L-Lactate Dehydrogenase