title: Effect of sodium alginate addition to resveratrol on acute gouty arthritis.
abstract: OBJECTIVE
  Resveratrol has been shown to exert anti-inflammatory and antioxidant effects, while sodium alginate is a common pharmaceutic adjuvant with antioxidative and immunomodulatory properties. We performed an animal study to investigate the effect of sodium alginate addition to resveratrol on acute gouty arthritis.
  
  
  METHODS
  Twenty-four SPF Wistar mice were randomized to four groups receiving the combination of sodium alginate and resveratrol, resveratrol alone, colchicine, and placebo, respectively. Acute gouty arthritis was induced by injection of 0.05 ml monosodium urate (MSU) solution (25g/mL) into ankle joint cavity. IL-1β, CCR5, and CXCL10 levels in both serum and synovial fluid were measured using ELISA. NLRP3 expression in the synovial tissues was measured using western plot.
  
  
  RESULTS
  The combination of sodium alginate and resveratrol significantly reduced synovial levels of IL-1β, CCR5, and CXCL10 when compared with colchicines, and all P values were less than 0.0001. The combination of sodium alginate and resveratrol was also superior to resveratrol in terms of both serum levels and synovial levels of IL-1β, CCR5, and CXCL10. In addition, resveratrol, with or without sodium alginate, could reduce NLRP3 expression obviously in the synovial tissues.
  
  
  CONCLUSIONS
  The combination of sodium alginate and resveratrol has better effect over colchicines in treating MSU-induced acute gouty arthritis.
authors.affiliation: Department of Endocrinology, Chengyang People's Hospital of Qingdao City, Qingdao, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.forename: Dunlin
authors.initials: D
authors.lastname: Ren
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Meiju
authors.initials: M
authors.lastname: Jiang
authors.forename: Robin
authors.initials: R
authors.lastname: Wang
authors.forename: Yan-Gang
authors.initials: YG
authors.lastname: Wang
mesh_terms: D000464:Alginates / Q000008:administration & dosage* / Q000494:pharmacology; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000494:pharmacology; D000975:Antioxidants / Q000008:administration & dosage* / Q000494:pharmacology; D015210:Arthritis, Gouty / Q000097:blood / Q000188:drug therapy* / Q000209:etiology; D054357:Chemokine CXCL10 / Q000378:metabolism; D003078:Colchicine / Q000008:administration & dosage* / Q000494:pharmacology; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination; D020723:Glucuronic Acid / Q000008:administration & dosage / Q000494:pharmacology; D006603:Hexuronic Acids / Q000008:administration & dosage / Q000494:pharmacology; D053583:Interleukin-1beta / Q000378:metabolism; D051379:Mice; D019713:Receptors, CCR5 / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000008:administration & dosage* / Q000494:pharmacology; D014527:Uric Acid
chemical_list: D000464:Alginates; D000893:Anti-Inflammatory Agents; D000975:Antioxidants; C000592874:CCR5 protein, mouse; D054357:Chemokine CXCL10; C485433:Cxcl10 protein, mouse; D006603:Hexuronic Acids; C587672:IL1B protein, mouse; D053583:Interleukin-1beta; D019713:Receptors, CCR5; D013267:Stilbenes; D014527:Uric Acid; D020723:Glucuronic Acid; D000077185:Resveratrol; D003078:Colchicine