title: Population structure and genetic diversity of the perennial medicinal shrub Plumbago.
abstract: Knowledge of the natural genetic variation and structure in a species is important for developing appropriate conservation strategies. As genetic diversity analysis among and within populations of Plumbago zeylanica remains unknown, we aimed (i) to examine the patterns and levels of morphological and genetic variability within/among populations and ascertain whether these variations are dependent on geographical conditions; and (ii) to evaluate genetic differentiation and population structure within the species. A total of 130 individuals from 13 populations of P. zeylanica were collected, covering the entire distribution area of species across India. The genetic structure and variation within and among populations were evaluated using inter-simple sequence repeat (ISSR) and randomly amplified DNA polymorphism (RAPD) markers. High levels of genetic diversity and significantly high genetic differentiation were revealed by both the markers among all studied populations. High values of among-population genetic diversity were found, which accounted for 60 % of the total genetic variance. The estimators of genetic diversity were higher in northern and eastern populations than in southern and western populations indicating the possible loss of genetic diversity during the spread of this species to Southern India. Bayesian analysis, unweighted pair group method with arithmetic average cluster analysis and principal coordinates analysis all showed similar results. A significant isolation-by-distance pattern was revealed in P. zeylanica by ISSR (r = 0.413, P = 0.05) and RAPD (r = 0.279, P = 0.05) analysis. The results obtained suggest an urgent need for conservation of existing natural populations along with extensive domestication of this species for commercial purpose.
authors.affiliation: Department of Botany, Savitribai Phule Pune University, Ganeshkhind, Pune 411007, India.
authors.forename: Sayantan
authors.initials: S
authors.lastname: Panda
authors.affiliation: Department of Environmental Sciences, Indian Institute of Advanced Research, Koba Institutional Area, Gandhinagar 382007, India.
authors.forename: Dhiraj
authors.initials: D
authors.lastname: Naik
authors.affiliation: Department of Botany, Savitribai Phule Pune University, Ganeshkhind, Pune 411007, India kambleavinash2000@gmail.com.
authors.forename: Avinash
authors.initials: A
authors.lastname: Kamble
keywords: Genetic diversity; Plumbago zeylanica; molecular markers; population structure