title: The relationship between selected VDR, HFE and ALAD gene polymorphisms and several basic toxicological parameters among persons occupationally exposed to lead.
abstract: The aim of this study was to find a relationship between polymorphisms of ALAD rs1805313, rs222808, rs1139488, VDR FokI and HFE C282Y and H63D and basic toxicological parameters (lead and ZnPP blood concentration) in people occupationally exposed to lead. We collected data of 101 workers (age 25-63 years) directly exposed to lead. The toxicological lab tests included blood lead, cadmium and ZnPP concentration measurement and arsenic urine concentration measurement. Workers were genotyped for ALAD (rs1805313, rs222808, rs1139488), HFE (C282Y, H63D) and VDR (FokI). Individuals with the lead exposure and coexisting F allel in the locus Fok-I of VDR gene are suspected of higher zinc protoporphyrins concentrations. Workers exposed to the lead with the Y allel in the locus C282Y of the HFE gene are predisposed to lower ZnPP levels and individuals with coexisting H allel in the locus H63D HFE gene are predisposed to lower Pb-B levels. The T allel in the locus rs1805313 of the ALAD gene determines lower Pb-B and ZnPP levels in lead-exposed individuals. The heterozigosity of the locus rs2228083 of the ALAD gene has a strong predilection to higher Pb-B levels. The carriage of the C allel in the locus rs1139488 of the ALAD gene might determine higher Pb-B levels and the heterozigosity of the locus rs1139488 of the ALAD gene might result in higher ZnPP levels.
  
  
  CONCLUSIONS
  The study revealed relationship between VDR, HFE and ALAD genes polymorphism and basic toxicological parameters in occupationally exposed workers.
authors.affiliation: Department of Internal Medicine, Occupational Diseases and Hypertension, Wroclaw Medical University, Borowska 213, PL 50-556 Wrocław, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Szymańska-Chabowska
authors.affiliation: Department of Endocrinology, Diabetology and Isotope Therapy, Wroclaw Medical University, Pasteura 4, PL 50-367 Wrocław, Poland.
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Łaczmański
authors.affiliation: Department of Internal Medicine, Occupational Diseases and Hypertension, Wroclaw Medical University, Borowska 213, PL 50-556 Wrocław, Poland.
authors.forename: Iwona
authors.initials: I
authors.lastname: Jędrychowska
authors.affiliation: Clinic of Surgery, 4th Military Hospital, Weigla 5, PL 50-981 Wrocław, Poland.
authors.forename: Mariusz
authors.initials: M
authors.lastname: Chabowski
authors.affiliation: Department of Internal Medicine, Occupational Diseases and Hypertension, Wroclaw Medical University, Borowska 213, PL 50-556 Wrocław, Poland; Department of Radiology and Diagnostic Imaging, 4th Military Hospital, Weigla 5, PL 50-981 Wrocław, Poland.
authors.forename: Paweł
authors.initials: P
authors.lastname: Gać
authors.affiliation: Department of Internal Medicine, Occupational Diseases and Hypertension, Wroclaw Medical University, Borowska 213, PL 50-556 Wrocław, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Janus
authors.affiliation: Department of Internal Medicine, Occupational Diseases and Hypertension, Wroclaw Medical University, Borowska 213, PL 50-556 Wrocław, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Gosławska
authors.affiliation: Department of Internal Medicine, Occupational Diseases and Hypertension, Wroclaw Medical University, Borowska 213, PL 50-556 Wrocław, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Smyk
authors.affiliation: Department of Internal Medicine, Occupational Diseases and Hypertension, Wroclaw Medical University, Borowska 213, PL 50-556 Wrocław, Poland.
authors.forename: Urszula
authors.initials: U
authors.lastname: Solska
authors.affiliation: Department of Internal Medicine, Occupational Diseases and Hypertension, Wroclaw Medical University, Borowska 213, PL 50-556 Wrocław, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Mazur
authors.affiliation: Department of Internal Medicine, Occupational Diseases and Hypertension, Wroclaw Medical University, Borowska 213, PL 50-556 Wrocław, Poland. Electronic address: sogood@poczta.onet.pl.
authors.forename: Rafał
authors.initials: R
authors.lastname: Poręba
mesh_terms: D000328:Adult; D001151:Arsenic / Q000652:urine; D015415:Biomarkers / Q000097:blood / Q000652:urine; D002104:Cadmium / Q000097:blood; D005260:Female; D020022:Genetic Predisposition to Disease; D000071020:Hemochromatosis Protein; D006579:Heterozygote; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006720:Homozygote; D006801:Humans; D007854:Lead / Q000009:adverse effects* / Q000097:blood; D007855:Lead Poisoning / Q000097:blood / Q000175:diagnosis / Q000235:genetics* / Q000652:urine; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D008875:Middle Aged; D009784:Occupational Diseases / Q000097:blood / Q000175:diagnosis / Q000235:genetics* / Q000652:urine; D016273:Occupational Exposure / Q000009:adverse effects*; D010641:Phenotype; D011110:Polymorphism, Genetic; D000623:Porphobilinogen Synthase / Q000235:genetics*; D011524:Protoporphyrins / Q000097:blood; D018167:Receptors, Calcitriol / Q000235:genetics*; D012307:Risk Factors
chemical_list: D015415:Biomarkers; C103673:HFE protein, human; D000071020:Hemochromatosis Protein; D015395:Histocompatibility Antigens Class I; D008565:Membrane Proteins; D011524:Protoporphyrins; D018167:Receptors, Calcitriol; C579457:VDR protein, human; D002104:Cadmium; C017803:zinc protoporphyrin; D007854:Lead; D000623:Porphobilinogen Synthase; D001151:Arsenic
keywords: Gene polymorphisms; Lead; Occupationally exposure