title: Barley Stripe Mosaic Virus (BSMV) Induced MicroRNA Silencing in Common Wheat (Triticum aestivum L.).
abstract: MicroRNAs (miRNAs) play important roles in growth, development, and response to environmental changes in plants. Based on the whole-genome shotgun sequencing strategy, more and more wheat miRNAs have been annotated. Now, there is a need for an effective technology to analyse endogenous miRNAs function in wheat. We report here that the modified barley stripe mosaic virus (BSMV)-induced miRNAs silencing system can be utilized to silence miRNAs in wheat. BSMV-based miRNA silencing system is performed through BSMV-based expression of miRNA target mimics to suppress miR159a and miR3134a. The relative expression levels of mature miR159a and miR3134a decrease with increasing transcript levels of their target genes in wheat plants. In summary, the developed approach is effective in silencing endogenous miRNAs, thereby providing a powerful tool for biological function analyses of miRNA molecules in common wheat.
authors.affiliation: Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, Beijing, P.R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Jiao
authors.affiliation: Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, Beijing, P.R. China.
authors.forename: Yichun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, Beijing, P.R. China.
authors.forename: Jonathan Nimal
authors.initials: JN
authors.lastname: Selvaraj
authors.affiliation: Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, Beijing, P.R. China.
authors.forename: Fuguo
authors.initials: F
authors.lastname: Xing
authors.affiliation: Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, Beijing, P.R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
mesh_terms: D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D017343:Genes, Plant; D005822:Genetic Vectors; D035683:MicroRNAs / Q000037:antagonists & inhibitors*; D009029:Mosaic Viruses / Q000235:genetics / Q000502:physiology*; D018749:RNA, Plant / Q000037:antagonists & inhibitors; D014908:Triticum / Q000235:genetics* / Q000821:virology
chemical_list: D035683:MicroRNAs; D018749:RNA, Plant