title: Comparative proteomics of root plasma membrane proteins reveals the involvement of calcium signalling in NaCl-facilitated nitrate uptake in Salicornia europaea.
abstract: Improving crop nitrogen (N) use efficiency under salinity is essential for the development of sustainable agriculture in marginal lands. Salicornia europaea is a succulent euhalophyte that can survive under high salinity and N-deficient habitat conditions, implying that a special N assimilation mechanism may exist in this plant. In this study, phenotypic and physiological changes of S. europaea were investigated under different nitrate and NaCl levels. The results showed that NaCl had a synergetic effect with nitrate on the growth of S. europaea. In addition, the shoot nitrate concentration and nitrate uptake rate of S. europaea were increased by NaCl treatment under both low N and high N conditions, suggesting that nitrate uptake in S. europaea was NaCl facilitated. Comparative proteomic analysis of root plasma membrane (PM) proteins revealed 81 proteins, whose abundance changed significantly in response to NaCl and nitrate. These proteins are involved in metabolism, cell signalling, transport, protein folding, membrane trafficking, and cell structure. Among them, eight proteins were calcium signalling components, and the accumulation of seven of the above-mentioned proteins was significantly elevated by NaCl treatment. Furthermore, cytosolic Ca(2+) concentration ([Ca(2+)]cyt) was significantly elevated in S. europaea under NaCl treatment. The application of the Ca(2+) channel blocker LaCl3 not only caused a decrease in nitrate uptake rate, but also attenuated the promoting effects of NaCl on nitrate uptake rates. Based on these results, a possible regulatory network of NaCl-facilitated nitrate uptake in S. europaea focusing on the involvement of Ca(2+) signalling was proposed.
authors.affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, PR China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Nie
authors.affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, PR China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Feng
authors.affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, PR China Department of Biochemistry and Molecular Biology, Michigan State University, 603 Wilson Road, East Lansing, MI 48824, USA.
authors.forename: Pengxiang
authors.initials: P
authors.lastname: Fan
authors.affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, PR China.
authors.forename: Xianyang
authors.initials: X
authors.lastname: Chen
authors.affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Guo
authors.affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, PR China.
authors.forename: Sulian
authors.initials: S
authors.lastname: Lv
authors.affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, PR China Shanghai Center for Plant Stress Biology (PSC), Chinese Academy of Sciences, No. 3888 Chenhua Road, Songjiang District, Shanghai 201602, PR China.
authors.forename: Hexigeduleng
authors.initials: H
authors.lastname: Bao
authors.affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, PR China.
authors.forename: Weitao
authors.initials: W
authors.lastname: Jia
authors.affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Tai
authors.affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, PR China.
authors.forename: Jinhui
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, PR China yxli@ibcas.ac.cn.
authors.forename: Yinxin
authors.initials: Y
authors.lastname: Li
mesh_terms: D020013:Calcium Signaling / Q000187:drug effects*; D002462:Cell Membrane / Q000378:metabolism; D019663:Chenopodiaceae / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D009566:Nitrates / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism; D040901:Proteomics; D055051:Salt-Tolerant Plants / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology*
chemical_list: D008565:Membrane Proteins; D009566:Nitrates; D010940:Plant Proteins; D012965:Sodium Chloride
keywords: 2D-DIGE; NaCl; Salicornia europaea.; [Ca2+]cyt; calcium signalling; nitrate uptake; plasma membrane