title: [Expression of Ki-67 in chronic lymphocytic leukemia and its clinical significance].
abstract: OBJECTIVE
  To investigate the expression of nuclear antigen Ki-67 in CLL patients and realationship of Ki-67 expression with other clinical parameters.
  
  
  METHODS
  Twenty-Six confirmed cases of CLL were analysised retrospectively. The immhnohistochemical method was carried out to examine the expression of Ki-67 in bone marrow cells, the flow cytometer was used to detect ZAP70 (Zeta chain-associated protein), CD38 and other markers, additionally, a panel of probes RB1 (13q14), ATM (11q22.3), P53 (17p13.1) and CSP12 (+12) FISH were perfomed to detect the cytogenetic abnormalities.
  
  
  RESULTS
  Out of 26 patients, 15 cases (57.7%) showed positive expression of Ki-67, 11 cases (42.3%) showed negative expression of Ki-67, the average rate of Ki-67 positive expression was (10.86±7.36)%. The level of Ki-67 did not relate with sex, age, Hb, platelet, ZAP70, ATM. β2-MG, IgHV and P53, but related to the Rai staging (P=0.01, r=0.517), CD38 (P=0.02, r=0.469), 13q14 (P=0.021, r=-0.48), and there was statistically significant difference (P<0.05).
  
  
  CONCLUSIONS
  The Ki-67 level is higher in progressive stage of CLL and the Ki-67 expression is related with Rai staging, CD38, 13q14. The expression level of Ki-67 may be used as indicator for evaluation of CLL prognosis and guiding treatment for this disease.
authors.affiliation: Center of Blood Diseases, The First Affitiated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Autonomous Rejion, China.
authors.forename: Ming-Fang
authors.initials: MF
authors.lastname: Tan
authors.affiliation: Center of Blood Diseases, The First Affitiated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Autonomous Rejion, China.
authors.forename: Zheng-Hao
authors.initials: ZH
authors.lastname: Zhang
authors.affiliation: Center of Blood Diseases, The First Affitiated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Autonomous Rejion, China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Yu
authors.affiliation: Center of Blood Diseases, The First Affitiated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Autonomous Rejion, China.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Qu
mesh_terms: D001854:Bone Marrow Cells; D002869:Chromosome Aberrations; D005434:Flow Cytometry; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D019394:Ki-67 Antigen; D015451:Leukemia, Lymphocytic, Chronic, B-Cell; D011379:Prognosis; D051746:ZAP-70 Protein-Tyrosine Kinase
chemical_list: D019394:Ki-67 Antigen; D051746:ZAP-70 Protein-Tyrosine Kinase; C497261:ZAP70 protein, human