title: Structures of prostaglandin F synthase from the protozoa Leishmania major and Trypanosoma cruzi with NADP.
abstract: The crystal structures of prostaglandin F synthase (PGF) from both Leishmania major and Trypanosoma cruzi with and without their cofactor NADP have been determined to resolutions of 2.6 Å for T. cruzi PGF, 1.25 Å for T. cruzi PGF with NADP, 1.6 Å for L. major PGF and 1.8 Å for L. major PGF with NADP. These structures were determined by molecular replacement to a final R factor of less than 18.6% (Rfree of less than 22.9%). PGF in the infectious protozoa L. major and T. cruzi is a potential therapeutic target.
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease, USA.
authors.forename: Spencer O
authors.initials: SO
authors.lastname: Moen
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease, USA.
authors.forename: James W
authors.initials: JW
authors.lastname: Fairman
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease, USA.
authors.forename: Steve R
authors.initials: SR
authors.lastname: Barnes
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease, USA.
authors.forename: Amy
authors.initials: A
authors.lastname: Sullivan
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease, USA.
authors.forename: Stephen
authors.initials: S
authors.lastname: Nakazawa-Hewitt
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease, USA.
authors.forename: Wesley C
authors.initials: WC
authors.lastname: Van Voorhis
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease, USA.
authors.forename: Bart L
authors.initials: BL
authors.lastname: Staker
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease, USA.
authors.forename: Donald D
authors.initials: DD
authors.lastname: Lorimer
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease, USA.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Myler
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease, USA.
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Edwards
mesh_terms: D000595:Amino Acid Sequence; D003460:Crystallization; D006801:Humans; D006911:Hydroxyprostaglandin Dehydrogenases / Q000737:chemistry* / Q000235:genetics; D018320:Leishmania major / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D009249:NADP / Q000737:chemistry* / Q000235:genetics; D017433:Protein Structure, Secondary; D014349:Trypanosoma cruzi / Q000737:chemistry* / Q000235:genetics
chemical_list: D009249:NADP; D006911:Hydroxyprostaglandin Dehydrogenases; C031051:prostaglandin-F synthase
keywords: Chagas disease; SSGCID; infectious diseases; leishmaniasis; prostaglandin