title: p16/CDKN2A FISH in Differentiation of Diffuse Malignant Peritoneal Mesothelioma From Mesothelial Hyperplasia and Epithelial Ovarian Cancer.
abstract: OBJECTIVE
  It can be difficult to differentiate diffuse malignant peritoneal mesothelioma (DMPM) from reactive mesothelial hyperplasia (RMH) or peritoneal dissemination of gynecologic malignancies, such as epithelial ovarian cancer (EOC), which cause a large amount of ascites. Detection of the homozygous deletion of p16/CDKN2A (p16) by fluorescence in situ hybridization (FISH) is an effective adjunct in the diagnosis of malignant pleural mesothelioma. The aim of this study was to investigate the ability of the p16 FISH assay to differentiate DMPM from RMH and EOC.
  
  
  METHODS
  p16 FISH was performed in 28 DMPMs (successful in 19), 30 RMHs, and 40 EOC cases. The cutoff values of p16 FISH were more than 10% for homozygous deletion and more than 40% for heterozygous deletion.
  
  
  RESULTS
  According to the above criteria, nine (47.4%) of 19 successful DMPM cases were homozygous deletion positive, and three (15.8%) of 19 were heterozygous deletion positive, whereas all RMH cases were negative for the p16 deletion. In all four major histologic subtypes of EOC, neither p16 homozygous nor heterozygous deletions were detected. To differentiate DMPM from RMH or EOC, the sensitivity of the p16 homozygous deletion was 32% (9/28), and the specificity was 100%.
  
  
  CONCLUSIONS
  Our study suggests that p16 FISH analysis is useful in differentiating DMPM from RMH and EOC when homozygous deletion is detected.
authors.affiliation: From the Department of Pathology and Department of Obstetrics and Gynecology, Fukuoka University School of Medicine and Hospital, Fukuoka, Japan;
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Ito
authors.affiliation: From the Department of Pathology and.
authors.forename: Makoto
authors.initials: M
authors.lastname: Hamasaki
authors.affiliation: From the Department of Pathology and.
authors.forename: Shinji
authors.initials: S
authors.lastname: Matsumoto
authors.affiliation: Department of Pathology, Tokyo Women's Medical University Yachiyo Medical Center, Yachiyo, Japan;
authors.forename: Kenzo
authors.initials: K
authors.lastname: Hiroshima
authors.affiliation: Department of Pathology, Hyogo College of Medicine, Hyogo, Japan;
authors.forename: Tohru
authors.initials: T
authors.lastname: Tsujimura
authors.affiliation: Department of Pathology and Laboratory Medicine, National Defense Medical College, Tokorozawa, Japan;
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Kawai
authors.affiliation: Department of Pathology, Miyazaki Prefectural Miyazaki Hospital, Miyazaki, Japan;
authors.forename: Yoshiya
authors.initials: Y
authors.lastname: Shimao
authors.affiliation: Department of Pathology, Miyazaki University School of Medicine, Miyazaki, Japan; and.
authors.forename: Kousuke
authors.initials: K
authors.lastname: Marutsuka
authors.affiliation: Department of Pathology, Miyazaki University School of Medicine, Miyazaki, Japan; and.
authors.forename: Sayaka
authors.initials: S
authors.lastname: Moriguchi
authors.affiliation: Laboratory of Surgical Pathology, Shimane University School of Medicine, Izumo, Japan.
authors.forename: Riruke
authors.initials: R
authors.lastname: Maruyama
authors.affiliation: Department of Obstetrics and Gynecology, Fukuoka University School of Medicine and Hospital, Fukuoka, Japan;
authors.forename: Shingo
authors.initials: S
authors.lastname: Miyamoto
authors.affiliation: From the Department of Pathology and kaznabes@fukuoka-u.ac.jp.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Nabeshima
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics; D000077216:Carcinoma, Ovarian Epithelial; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics; D003937:Diagnosis, Differential; D005260:Female; D019942:Genes, p16; D006801:Humans; D006965:Hyperplasia / Q000175:diagnosis / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008654:Mesothelioma / Q000175:diagnosis* / Q000235:genetics; D008875:Middle Aged; D009375:Neoplasms, Glandular and Epithelial / Q000175:diagnosis* / Q000235:genetics; D010051:Ovarian Neoplasms / Q000175:diagnosis* / Q000235:genetics; D010534:Peritoneal Neoplasms / Q000175:diagnosis* / Q000235:genetics; D011230:Precancerous Conditions / Q000175:diagnosis / Q000235:genetics; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; D019941:Cyclin-Dependent Kinase Inhibitor p16
keywords: 9p21; Diffuse malignant peritoneal mesothelioma; Epithelial ovarian cancer; Fluorescence in situ hybridization; Reactive mesothelial hyperplasia; p16