title: Vibronic Structures in Absorption and Fluorescence Spectra of Firefly Oxyluciferin in Aqueous Solutions.
abstract: To elucidate the factors determining the spectral shapes and widths of the absorption and fluorescence spectra for keto and enol oxyluciferin and their conjugate bases in aqueous solutions, the intensities of vibronic transitions between their ground and first electronic excited states were calculated for the first time via estimation of the vibrational Franck-Condon factors. The major normal modes, overtones and combination tones in absorption and fluorescence spectra are similar for all species. The theoretical full widths at half maximum of absorption spectra are 0.4-0.7 eV and those for the fluorescence spectra are 0.4-0.5 eV, except for phenolate-keto that exhibits exceptionally sharp peak widths due to the dominance of the 0-0' or 0'-0 band. These spectral shapes and widths explain many relevant features of the experimentally observed spectra.
authors.affiliation: Institute for Solid State Physics, The University of Tokyo, Kashiwa, Chiba, Japan.
authors.forename: Miyabi
authors.initials: M
authors.lastname: Hiyama
authors.affiliation: Institute for Solid State Physics, The University of Tokyo, Kashiwa, Chiba, Japan.
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Noguchi
authors.affiliation: Institute for Solid State Physics, The University of Tokyo, Kashiwa, Chiba, Japan.
authors.forename: Hidefumi
authors.initials: H
authors.lastname: Akiyama
authors.affiliation: Graduate School of Information Science, Nagoya University, Nagoya, Japan.
authors.forename: Kenta
authors.initials: K
authors.lastname: Yamada
authors.affiliation: Graduate School of Information Science, Nagoya University, Nagoya, Japan.
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Koga
mesh_terms: D000818:Animals; D001517:Coleoptera / Q000737:chemistry*; D007211:Indoles / Q000737:chemistry*; D015394:Molecular Structure; D011719:Pyrazines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D014867:Water
chemical_list: D007211:Indoles; D011719:Pyrazines; D014867:Water; C014730:oxyluciferin