title: Inhibition of ovarian cancer proliferation and invasion by pachymic acid.
abstract: To determine the effect of pachymic acid (PA) on proliferation, cell cycle, and invasion in human ovarian carcinoma cell lines HO-8910 and explore some possible mechanisms, HO-8910 cells was treated with different concentrations of PA (0.5, 1, 2 μM). CCK-8 assay, propidium iodide staining, was applied to measuring the growth inhibiting rates of HO-8910 cells. Cell cycle was measured by flow cytometry. In addition, the activity of PA against HO-8910 cells invasion was evaluated in transwell assay. Western blot detected the proteins expression of E-cadherin, β-catenin and COX-2 of different groups treated with PA in different concentrations (0.5, 1, 2 μM) for 48 h. Our results showed that PA could effectively inhibit the in vitro growth of HO-8910 cells in dose-dependent manners in 72 h, suppressed migration and invasion of HO-8910 cells in concentration-dependent manners at 24 h, caused the increased accumulation of G1 phase cells, and caused down-regulation of β-catenin and COX-2 and up-regulation of E-cadherin expression level. Taken together, it could conclude that PA might inhibit proliferation and invasion of ovarian carcinoma cell through decreasing β-catenin and COX-2 expression and increasing E-cadherin expression.
authors.affiliation: Department of Obstetrics and Gynecology, The 2nd Affiliated Hospital of Soochow University 1055 Sanxiang Road, Gusu District, Suzhou 215004, Jiangsu Province, China.
authors.forename: Ai-Hua
authors.initials: AH
authors.lastname: Gao
authors.affiliation: College of Pharmacy, Suzhou University 199 Renai Road, Industrial Park, Suzhou 215021, Jiangsu Province, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and Gynecology, The 2nd Affiliated Hospital of Soochow University 1055 Sanxiang Road, Gusu District, Suzhou 215004, Jiangsu Province, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Obstetrics and Gynecology, The 2nd Affiliated Hospital of Soochow University 1055 Sanxiang Road, Gusu District, Suzhou 215004, Jiangsu Province, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Obstetrics and Gynecology, The 2nd Affiliated Hospital of Soochow University 1055 Sanxiang Road, Gusu District, Suzhou 215004, Jiangsu Province, China.
authors.forename: Zhen-Zhen
authors.initials: ZZ
authors.lastname: Xu
authors.affiliation: Department of Obstetrics and Gynecology, The 2nd Affiliated Hospital of Soochow University 1055 Sanxiang Road, Gusu District, Suzhou 215004, Jiangsu Province, China.
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Liu
authors.affiliation: Department of Obstetrics and Gynecology, The 2nd Affiliated Hospital of Soochow University 1055 Sanxiang Road, Gusu District, Suzhou 215004, Jiangsu Province, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
mesh_terms: D015820:Cadherins / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D051546:Cyclooxygenase 2 / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D009361:Neoplasm Invasiveness / Q000473:pathology*; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology*; D014315:Triterpenes / Q000494:pharmacology*; D051176:beta Catenin / Q000378:metabolism
chemical_list: D015820:Cadherins; D014315:Triterpenes; D051176:beta Catenin; D051546:Cyclooxygenase 2; C102487:pachymic acid
keywords: HO-8910 cells; invasion; pachymic acid; proliferation