title: Evaluation of chromatographic columns packed with semi- and fully porous particles for benzimidazoles separation.
abstract: The behavior of 15 benzimidazoles, including their main metabolites, using several C18 columns with standard or narrow-bore diameters and different particle size and type were evaluated. These commercial columns were selected because their differences could affect separation of benzimidazoles, and so they can be used as alternative columns. A simple screening method for the analysis of benzimidazole residues and their main metabolites was developed. First, the separation of benzimidazoles was optimized using a Kinetex C18 column; later, analytical performances of other columns using the above optimized conditions were compared and then individually re-optimized. Critical pairs resolution, analysis run time, column type and characteristics, and selectivity were considered for chromatographic columns comparison. Kinetex XB was selected because it provides the shortest analysis time and the best resolution of critical pairs. Using this column, the separation conditions were re-optimized using a factorial design. Separations obtained with the different columns tested can be applied to the analysis of specific benzimidazoles residues or other applications.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, Universidad Complutense Madrid, Ciudad Universitaria, Madrid, Spain.
authors.forename: Raquel
authors.initials: R
authors.lastname: Gonzalo-Lumbreras
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, Universidad Complutense Madrid, Ciudad Universitaria, Madrid, Spain.
authors.forename: Jon
authors.initials: J
authors.lastname: Sanz-Landaluze
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, Universidad Complutense Madrid, Ciudad Universitaria, Madrid, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Cámara
mesh_terms: D001562:Benzimidazoles / Q000032:analysis* / Q000737:chemistry; D002623:Chemistry Techniques, Analytical; D002845:Chromatography / Q000295:instrumentation* / Q000379:methods; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008024:Ligands; D010316:Particle Size; D016062:Porosity; D025341:Principal Component Analysis; D015203:Reproducibility of Results; D012997:Solvents
chemical_list: D001562:Benzimidazoles; D008024:Ligands; D012997:Solvents
keywords: Benzimidazoles; Liquid chromatography; Porous packings