title: Enhanced expression of trim14 gene suppressed Sindbis virus reproduction and modulated the transcription of a large number of genes of innate immunity.
abstract: In the present research, we have studied an influence of enhanced expression TRIM14 on alphavirus Sindbis (SINV, Togaviridae family) infection. In the HEK293 cells transfected with human trim14 gene (HEK-trim14), SINV yield after infection was decreased 1000-10,000 times (3-4 lg of TCD50/ml) at 24 h p.i. and considerably less (1-2 lg of TCD50/ml) at 48 h p.i. Analysis of the expression of 43 genes directly or indirectly involved in innate immune machine in HEK-trim14 non-infected cells comparing with the control (non-transfected) HEK293 cells revealed that stable trim14 transfection in HEK293 cells caused increased transcription of 18 genes (ifna, il6 (ifnβ2), isg15, raf-1, NF-kB (nf-kb1, rela, nf-kb2, relb), grb2, grb3-3, traf3ip2, junB, c-myb, pu.1, akt1, tyk2, erk2, mek2) and lowered transcription of 3 genes (ifnγ, gata1, il-17a). The similar patterns of genes expression observe in SINV-infected non-transfected HEK293 cells. However, SINV infection of HEK-trim14 cells caused inhibition of the most interferon cascade genes as well as subunits of transcription factor NF-κB. Thus, stable enhanced expression of trim14 gene in cells activates the transcription of many immunity genes and suppresses the SINV reproduction, but SINV infection of HEK-trim14 cells promotes inhibition of some genes involved in innate immune system.
authors.affiliation: Institute of Molecular Genetics, Russian Academy of Sciences, 2 Kurchatov Square, 123182, Moscow, Russia, nenasheva@img.ras.ru.
authors.forename: V V
authors.initials: VV
authors.lastname: Nenasheva
authors.forename: G V
authors.initials: GV
authors.lastname: Kovaleva
authors.forename: L V
authors.initials: LV
authors.lastname: Uryvaev
authors.forename: K S
authors.initials: KS
authors.lastname: Ionova
authors.forename: A V
authors.initials: AV
authors.lastname: Dedova
authors.forename: G K
authors.initials: GK
authors.lastname: Vorkunova
authors.forename: S V
authors.initials: SV
authors.lastname: Chernyshenko
authors.forename: N V
authors.initials: NV
authors.lastname: Khaidarova
authors.forename: V Z
authors.initials: VZ
authors.lastname: Tarantul
mesh_terms: D018354:Alphavirus Infections / Q000276:immunology* / Q000821:virology; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics*; D002460:Cell Line; D057809:HEK293 Cells; D054884:Host-Pathogen Interactions / Q000276:immunology; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics*; D047908:Intracellular Signaling Peptides and Proteins; D012845:Sindbis Virus / Q000254:growth & development* / Q000276:immunology; D014162:Transfection; D000070584:Tripartite Motif Proteins; D014779:Virus Replication / Q000235:genetics*
chemical_list: D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; C481145:TRIM14 protein, human; D000070584:Tripartite Motif Proteins