title: Enzymatic synthesis of 2'-ara and 2'-deoxy analogues of c-di-GMP.
abstract: The substrate specificity of recombinant full-length diguanylate cyclase (DGC) of Thermotoga maritima with mutant allosteric site was investigated. It has been originally shown that the enzyme could use GTP closest analogues - 2'-deoxyguanosine-5'-triphosphate (dGTP) and 9-β-D-arabinofuranosyl-guanine-5'-triphosphate (araGTP) as the substrates. The first demonstrations of an enzymatic synthesis of bis-(3'-5')-cyclic dimeric deoxyguanosine monophosphate (c-di-dGMP) and the previously unknown bis-(3'-5')-cyclic dimeric araguanosine monophosphate (c-di-araGMP) using DGC of T. maritima in the form of inclusion bodies have been provided.
authors.affiliation: a Institute of Microbiology, National Academy of Sciences , Minsk , Belarus.
authors.forename: Anastasia S
authors.initials: AS
authors.lastname: Shchokolova
authors.forename: Alexander N
authors.initials: AN
authors.lastname: Rymko
authors.forename: Sergey V
authors.initials: SV
authors.lastname: Kvach
authors.forename: Polina S
authors.initials: PS
authors.lastname: Shabunya
authors.forename: Svetlana A
authors.initials: SA
authors.lastname: Fatykhava
authors.forename: Anatoly I
authors.initials: AI
authors.lastname: Zinchenko
mesh_terms: D001088:Arabinonucleotides / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry*; D006152:Cyclic GMP / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D003848:Deoxyguanine Nucleotides / Q000737:chemistry*; D029968:Escherichia coli Proteins / Q000737:chemistry*; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives* / Q000737:chemistry; D019761:Phosphorus-Oxygen Lyases / Q000737:chemistry*; D020124:Thermotoga maritima / Q000201:enzymology*
chemical_list: D001088:Arabinonucleotides; D001426:Bacterial Proteins; D003848:Deoxyguanine Nucleotides; D029968:Escherichia coli Proteins; C062025:bis(3',5')-cyclic diguanylic acid; C022206:9-beta-D-arabinofuranosylguanosine 5'-triphosphate; D006160:Guanosine Triphosphate; C029603:deoxyguanosine triphosphate; D019761:Phosphorus-Oxygen Lyases; C060720:diguanylate cyclase; D006152:Cyclic GMP
keywords: Bis-(3′-5′)-cyclic dimeric araguanosine monophosphate; bis-(3′-5′)-cyclic dimeric deoxyguanosine monophosphate; diguanylate cyclase; enzymatic synthesis; с-di-GMP derivatives