title: Differential MicroRNA Expression Profiles in Primary and Recurrent Epithelial Ovarian Cancer.
abstract: OBJECTIVE
  Although it has been shown that microRNAs influence messenger RNA post-transcriptional control and can attribute to human tumorigenesis, little is known regarading the differences in microRNA expression between primary and recurrent epithelial ovarian cancer (EOC). The purpose of the present study was to assess the differential expression of microRNA between primary and recurrent EOC.
  
  
  METHODS
  Between September 2013 and May 2014, the expression of microRNAs in tumor tissues from 5 primary and 5 recurrent EOC cases were analyzed. The tumor histotype was serous cystadenocarcinoma in all patients. Total RNA was extracted from tumor samples and microRNA expression levels were measured by performing microarray analysis. Expression levels were compared between the two groups and analyzed statistically.
  
  
  RESULTS
  Several microRNAs were differentially expressed in recurrent EOC compared to primary EOC, including 18 under-expressed microRNAs and 33 over-expressed microRNAs among 6,658 human microRNAs. Four specific microRNAs were the most significantly over-expressed in recurrent EOC: miR-551b, miR-19b, miR-196b and miR-3198. Moreover, 4 specific microRNAs were the most significantly down-expressed in recurrent EOC: miR-8084, miR-3201, miR-3613 and miR-7515.
  
  
  CONCLUSIONS
  Based on our data, dysregulation of microRNA expression was associated with the recurrence of EOC. Moreover, significantly over- and down-regulated microRNAs can be useful biomarkers for the prediction of recurrence in EOC.
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Kyungpook National University Medical Center, Daegu, Republic of Korea.
authors.forename: Gun Oh
authors.initials: GO
authors.lastname: Chong
authors.affiliation: Molecular Diagnostics and Imaging Center, School of Medicine, Kyungpook National University, Daegu, Republic of Korea.
authors.forename: Hyo-Sung
authors.initials: HS
authors.lastname: Jeon
authors.affiliation: Department of Physiology, School of Medicine, Kyungpook National University Medical Center, Daegu, Republic of Korea.
authors.forename: Hyung Soo
authors.initials: HS
authors.lastname: Han
authors.affiliation: Department of Internal Medicine, Konyang University Hospital, Daejeon, Republic of Korea.
authors.forename: Ji Woong
authors.initials: JW
authors.lastname: Son
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Kyungpook National University Medical Center, Daegu, Republic of Korea.
authors.forename: Yoon Hee
authors.initials: YH
authors.lastname: Lee
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Kyungpook National University Medical Center, Daegu, Republic of Korea.
authors.forename: Dae Gy
authors.initials: DG
authors.lastname: Hong
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Kyungpook National University Medical Center, Daegu, Republic of Korea.
authors.forename: Yoon Soon
authors.initials: YS
authors.lastname: Lee
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Kyungpook National University Medical Center, Daegu, Republic of Korea ylcho@knu.ac.kr.
authors.forename: Young Lae
authors.initials: YL
authors.lastname: Cho
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor; D000077216:Carcinoma, Ovarian Epithelial; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000235:genetics* / Q000473:pathology; D009375:Neoplasms, Glandular and Epithelial / Q000235:genetics* / Q000473:pathology; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology; D011379:Prognosis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; D012333:RNA, Messenger
keywords: Epithelial ovarian cancer; microRNA; recurrence