title: Full genomic characterization and phylogenetic analysis of a zoonotic human G8P[14] rotavirus strain detected in a sample from Guatemala.
abstract: We report the genomic characterization of a rare human G8P[14] rotavirus strain, identified in a stool sample from Guatemala (GTM) during routine rotavirus surveillance. This strain was designated as RVA/Human-wt/GTM/2009726790/2009/G8P[14], with a genomic constellation of G8-P[14]-I2-R2-C2-M2-A13-N2-T6-E2-H3. The VP4 gene occupied lineage VII within the P[14] genotype. Phylogenetic analysis of each genome segment revealed close relatedness to several zoonotic simian, guanaco and bovine strains. Our findings suggest that strain RVA/Human-wt/GTM/2009726790/2009/G8P[14] is an example of a direct zoonotic transmission event. The results of this study reinforce the potential role of interspecies transmission and reassortment in generating novel and rare rotavirus strains which infect humans.
authors.affiliation: Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA. Electronic address: ijs0@cdc.gov.
authors.forename: Rashi
authors.initials: R
authors.lastname: Gautam
authors.affiliation: Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Slavica
authors.initials: S
authors.lastname: Mijatovic-Rustempasic
authors.affiliation: Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Sunando
authors.initials: S
authors.lastname: Roy
authors.affiliation: Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Mathew D
authors.initials: MD
authors.lastname: Esona
authors.affiliation: Center for Health Studies, Universidad del Valle de Guatemala, Guatemala City, Guatemala.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Lopez
authors.affiliation: Laboratorio Nacional de Salud, Guatemala City, Guatemala.
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Mencos
authors.affiliation: Pan American Health Organization, Washington, DC, USA.
authors.forename: Gloria
authors.initials: G
authors.lastname: Rey-Benito
authors.affiliation: Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Michael D
authors.initials: MD
authors.lastname: Bowen
mesh_terms: D000818:Animals; D005814:Genes, Viral; D016679:Genome, Viral; D023281:Genomics; D006166:Guatemala / Q000453:epidemiology; D006801:Humans; D016366:Open Reading Frames; D010802:Phylogeny; D012401:Rotavirus / Q000145:classification* / Q000235:genetics*; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000635:transmission / Q000821:virology*; D015047:Zoonoses / Q000821:virology
keywords: G8P[14]; Interspecies transmission; Reassortant; Rotavirus