title: Apical extrusion of Enterococcus faecalis using three different rotary instrumentation techniques: an in vitro study.
abstract: OBJECTIVE
  To compare the apical extrusion of Enterococcus faecalis after instrumentation with three different Ni-Ti rotary instruments- An in vitro study.
  
  
  METHODS
  In vitro study Methods and Material: Forty freshly extracted mandibular premolars were mounted in bacteria collection apparatus and root canals were contaminated with a suspension of Enterococcus faecalis. The contaminated teeth were divided into 4 groups of 10 teeth each according to rotary system used for instrumentation: Group1: Hyflex files, Group 2: GTX files, Group 3: Protaper files and Group 4: control group (no instrumentation). Bacteria extruded after preparations were collected into vials and microbiological samples were incubated in BHI broth for 24 hrs. The colony forming units were determined for each sample.
  
  
  METHODS
  Statistical analysis was done using one way ANOVA followed by post hoc independent " t" test.
  
  
  RESULTS
  GTX files extruded least amount of bacteria followed by Hyflex files. Maximum extrusion of E. faecalis was seen in rotary Protaper group.
  
  
  CONCLUSIONS
  Least amount of extrusion was seen with GTX files followed by Hyflex files and then rotary Protaper system.
authors.affiliation: Department of Conservative Dentistry and Endodontics, I.T.S Centre for Dental Studies and Research, Ghaziabad, Uttar Pradesh, India.
authors.forename: Sonali
authors.initials: S
authors.lastname: Taneja
authors.forename: Manju
authors.initials: M
authors.lastname: Kumari
authors.forename: Madhumita
authors.initials: M
authors.lastname: Barua
authors.forename: Chetna
authors.initials: C
authors.lastname: Dudeja
authors.forename: Meeta
authors.initials: M
authors.lastname: Malik
mesh_terms: D001641:Bicuspid / Q000382:microbiology* / Q000601:surgery*; D003762:Dental Instruments; D003786:Dental Pulp Cavity / Q000382:microbiology; D013293:Enterococcus faecalis; D006801:Humans; D066298:In Vitro Techniques; D018915:Root Canal Preparation / Q000295:instrumentation*; D012399:Rotation; D013234:Stem Cells; D019227:Tooth Apex / Q000382:microbiology*; D014081:Tooth Extraction