title: Glycopeptidolipid of Mycobacterium smegmatis J15cs Affects Morphology and Survival in Host Cells.
abstract: Mycobacterium smegmatis has been widely used as a mycobacterial infection model. Unlike the M. smegmatis mc(2)155 strain, M. smegmatis J15cs strain has the advantage of surviving for one week in murine macrophages. In our previous report, we clarified that the J15cs strain has deleted apolar glycopeptidolipids (GPLs) in the cell wall, which may affect its morphology and survival in host cells. In this study, the gene causing the GPL deletion in the J15cs strain was identified. The mps1-2 gene (MSMEG_0400-0402) correlated with GPL biosynthesis. The J15cs strain had 18 bps deleted in the mps1 gene compared to that of the mc(2)155 strain. The mps1-complemented J15cs mutant restored the expression of GPLs. Although the J15cs strain produces a rough and dry colony, the colony morphology of this mps1-complement was smooth like the mc(2)155 strain. The length in the mps1-complemented J15cs mutant was shortened by the expression of GPLs. In addition, the GPL-restored J15cs mutant did not survive as long as the parent J15cs strain in the murine macrophage cell line J774.1 cells. The results are direct evidence that the deletion of GPLs in the J15cs strain affects bacterial size, morphology, and survival in host cells.
authors.affiliation: Department of Food and Nutrition, Faculty of Contemporary Human Life Science, Tezukayama University, Nara City, Nara, Japan; Department of Bacteriology, Osaka City University Graduate School of Medicine, Osaka City, Osaka, Japan.
authors.forename: Nagatoshi
authors.initials: N
authors.lastname: Fujiwara
authors.affiliation: Department of Oral Microbiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama City, Okayama, Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Ohara
authors.affiliation: Department of Microbiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu City, Fukuoka, Japan.
authors.forename: Midori
authors.initials: M
authors.lastname: Ogawa
authors.affiliation: Molecular Epidemiology Division, Mycobacterium Reference Center, The Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, Kiyose City, Tokyo, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Maeda
authors.affiliation: Department of Bacteriology, Osaka City University Graduate School of Medicine, Osaka City, Osaka, Japan; MBR Co. Ltd., Toyonaka City, Osaka, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Naka
authors.affiliation: Department of Microbiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu City, Fukuoka, Japan.
authors.forename: Hatsumi
authors.initials: H
authors.lastname: Taniguchi
authors.affiliation: Japan BCG Laboratory, Kiyose City, Tokyo, Japan.
authors.forename: Saburo
authors.initials: S
authors.lastname: Yamamoto
authors.affiliation: Department of Virology, Osaka City University Graduate School of Medicine, Osaka City, Osaka, Japan.
authors.forename: Minoru
authors.initials: M
authors.lastname: Ayata
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000502:physiology*; D002460:Cell Line; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D006017:Glycolipids / Q000502:physiology*; D006020:Glycopeptides / Q000502:physiology*; D054884:Host-Pathogen Interactions; D051379:Mice; D050296:Microbial Viability; D020102:Mycobacterium smegmatis / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D006017:Glycolipids; D006020:Glycopeptides