title: Up-regulation of BRAF activated non-coding RNA is associated with radiation therapy for lung cancer.
abstract: Radiation therapy has become more effective in treating primary tumors, such as lung cancer. Recent evidence suggested that BRAF activated non-coding RNAs (BANCR) play a critical role in cellular processes and are found to be dysregulated in a variety of cancers. The clinical significance of BANCR in radiation therapy, and its molecular mechanisms controlling tumor growth are unclear. In the present study, C57BL/6 mice were inoculated Lewis lung cancer cells and exposed to radiation therapy, then BANCR expression was analyzed using qPCR. Chromatin immunoprecipitation and western blot were performed to calculate the enrichment of histone acetylation and HDAC3 protein levels in Lewis lung cancer cells, respectively. MTT assay was used to evaluate the effects of BANCR on Lewis lung cancer cell viability. Finally, we found that BANCR expression was significantly increased in C57BL/6 mice receiving radiation therapy (P<0.05) compared with control group. Additionally, knockdown of BANCR expression was associated with larger tumor size in C57BL/6 mice inoculated Lewis lung cancer cells. Histone deacetylation was observed to involve in the regulation of BANCR in Lewis lung cancer cells. Moreover, over expression HDAC3 reversed the effect of rays on BANCR expression. MTT assay showed that knockdown of BANCR expression promoted cell viability surviving from radiation. In conclusion, these findings indicated that radiation therapy was an effective treatment for lung cancer, and it may exert function through up-regulation BANCR expression.
authors.affiliation: Department of Radiation Oncology, Zhejiang Cancer Hospital, Key Laboratory of Radiation Oncology of Zhejiang Province, Hangzhou 310022, China.
authors.forename: Jian-xiang
authors.initials: JX
authors.lastname: Chen
authors.affiliation: Department of Radiation Oncology, Zhejiang Cancer Hospital, Key Laboratory of Radiation Oncology of Zhejiang Province, Hangzhou 310022, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Chen
authors.affiliation: Department of Radiation Oncology, Zhejiang Cancer Hospital, Key Laboratory of Radiation Oncology of Zhejiang Province, Hangzhou 310022, China.
authors.forename: Yuan-da
authors.initials: YD
authors.lastname: Zheng
authors.affiliation: Department of Radiation Oncology, Zhejiang Cancer Hospital, Key Laboratory of Radiation Oncology of Zhejiang Province, Hangzhou 310022, China.
authors.forename: Sheng-ye
authors.initials: SY
authors.lastname: Wang
authors.affiliation: Department of Thoracic Surgery, Zhejiang Cancer Hospital, Key Laboratory of Radiation Oncology of Zhejiang Province, Hangzhou 310022, China. Electronic address: ZhupingShen423@126.com.
authors.forename: Zhu-ping
authors.initials: ZP
authors.lastname: Shen
mesh_terms: D000818:Animals; D018827:Carcinoma, Lewis Lung / Q000235:genetics / Q000532:radiotherapy; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects; D055785:Gene Knockdown Techniques; D006655:Histone Deacetylases / Q000378:metabolism; D008175:Lung Neoplasms / Q000235:genetics* / Q000532:radiotherapy*; D008810:Mice, Inbred C57BL; D048493:Proto-Oncogene Proteins B-raf / Q000378:metabolism*; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D047368:Tumor Burden / Q000528:radiation effects; D015854:Up-Regulation / Q000235:genetics* / Q000528:radiation effects; D014965:X-Rays
chemical_list: D062085:RNA, Long Noncoding; D048493:Proto-Oncogene Proteins B-raf; D006655:Histone Deacetylases
keywords: BRAF activated non-coding RNA; HDAC3; Histone acetylation; Lewis lung cancer cell; Lung cancer; Radiation therapy