title: Developmental Competence of Vitrified-Warmed Bovine Oocytes at the Germinal-Vesicle Stage is Improved by Cyclic Adenosine Monophosphate Modulators during In Vitro Maturation.
abstract: Cryopreservation of mature oocytes and embryos has provided numerous benefits in reproductive medicine. Although successful cryopreservation of germinal-vesicle stage (GV) oocytes holds promise for further advances in reproductive biology and clinical embryology fields, reports regarding cryopreservation of immature oocytes are limited. Oocyte survival and maturation rates have improved since vitrification is being performed at the GV stage, but the subsequent developmental competence of GV oocytes is still low. The purpose of this study was to evaluate the effects of supplementation of the maturation medium with cyclic adenosine monophosphate (cAMP) modulators on the developmental competence of vitrified-warmed GV bovine oocytes. GV oocytes were vitrified-warmed and cultured to allow for oocyte maturation, and then parthenogenetically activated or fertilized in vitro. Our results indicate that addition of a cAMP modulator forskolin (FSK) or 3-isobutyl-1-methylxanthine (IBMX) to the maturation medium significantly improved the developmental competence of vitrified-warmed GV oocytes. We also demonstrated that vitrification of GV oocytes led to a decline in cAMP levels and maturation-promoting factor (MPF) activity in the oocytes during the initial and final phases of maturation, respectively. Nevertheless, the addition of FSK or IBMX to the maturation medium significantly elevated cAMP levels and MPF activity during IVM. Taken together, our results suggest that the cryopreservation-associated meiotic and developmental abnormalities observed in GV oocytes may be ameliorated by an artificial increase in cAMP levels during maturation culture after warming.
authors.affiliation: Kato Ladies Clinic, Shinjuku-ku, Tokyo, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Ezoe
authors.affiliation: Kato Ladies Clinic, Shinjuku-ku, Tokyo, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Yabuuchi
authors.affiliation: Laboratory of Animal Reproduction, College of Agriculture, Kinki University, Nara, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Tani
authors.affiliation: Kato Ladies Clinic, Shinjuku-ku, Tokyo, Japan.
authors.forename: Chiemi
authors.initials: C
authors.lastname: Mori
authors.affiliation: Kato Ladies Clinic, Shinjuku-ku, Tokyo, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Miki
authors.affiliation: Kato Ladies Clinic, Shinjuku-ku, Tokyo, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Takayama
authors.affiliation: Sher Institute for Reproductive Medicine-Las Vegas, Las Vegas, NV, United States of America.
authors.forename: Zeki
authors.initials: Z
authors.lastname: Beyhan
authors.affiliation: Laboratory of Animal Reproduction, College of Agriculture, Kinki University, Nara, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Kato
authors.affiliation: Kato Ladies Clinic, Shinjuku-ku, Tokyo, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Okuno
authors.affiliation: Kato Ladies Clinic, Shinjuku-ku, Tokyo, Japan.
authors.forename: Tamotsu
authors.initials: T
authors.lastname: Kobayashi
authors.affiliation: Kato Ladies Clinic, Shinjuku-ku, Tokyo, Japan.
authors.forename: Keiichi
authors.initials: K
authors.lastname: Kato
mesh_terms: D015056:1-Methyl-3-isobutylxanthine / Q000008:administration & dosage; D000818:Animals; D002417:Cattle; D005576:Colforsin / Q000008:administration & dosage; D015925:Cryopreservation; D000242:Cyclic AMP / Q000378:metabolism*; D005260:Female; D005307:Fertilization in Vitro; D059471:In Vitro Oocyte Maturation Techniques; D009865:Oocytes / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D058989:Vitrification / Q000187:drug effects
chemical_list: D005576:Colforsin; D000242:Cyclic AMP; D015056:1-Methyl-3-isobutylxanthine