title: Antioxidant Activity of Oxygen Evolving Enhancer Protein 1 Purified from Capsosiphon fulvescens.
abstract: This study was conducted to determine the antioxidant activity of a protein purified from Capsosiphon fulvescens. The purification steps included sodium acetate (pH 6) extraction and diethylaminoethyl-cellulose, reversed phase Shodex C4P-50 column chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis indicated that the molecular weight of the purified protein was 33 kDa. The N-terminus and partial peptide amino acid sequence of this protein was identical to the sequence of oxygen evolving enhancer (OEE) 1 protein. The antioxidant activity of the OEE 1 was determined in vitro using a scavenging test with 4 types of reactive oxygen species (ROS), including the 2,2-diphenyl-1-picrylhydrazyl radical, hydroxyl radical, superoxide anion, and hydrogen peroxide (H2 O2 ). OEE 1 had higher H2 O2 scavenging activity, which proved to be the result of enzymatic antioxidants rather than nonenzymatic antioxidants. In addition, OEE 1 showed less H2 O2 -mediated ROS formation in HepG2 cells. In conclusion, this study demonstrates that OEE 1 purified from C. fulvescens is an excellent antioxidant.
authors.affiliation: Inst. of Fisheries Sciences, Pukyong Natl. Univ., Ilgwang-ro, Ilgwang-myeon, Gijang-gun, Busan, 619-911, Republic of Korea.
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Kim
authors.affiliation: Inst. of Fisheries Sciences, Pukyong Natl. Univ., Ilgwang-ro, Ilgwang-myeon, Gijang-gun, Busan, 619-911, Republic of Korea.
authors.forename: Youn Hee
authors.initials: YH
authors.lastname: Choi
authors.affiliation: Inst. of Fisheries Sciences, Pukyong Natl. Univ., Ilgwang-ro, Ilgwang-myeon, Gijang-gun, Busan, 619-911, Republic of Korea.
authors.forename: Jung Im
authors.initials: JI
authors.lastname: Lee
authors.affiliation: Inst. of Fisheries Sciences, Pukyong Natl. Univ., Ilgwang-ro, Ilgwang-myeon, Gijang-gun, Busan, 619-911, Republic of Korea.
authors.forename: In-Hye
authors.initials: IH
authors.lastname: Kim
authors.affiliation: Inst. of Fisheries Sciences, Pukyong Natl. Univ., Ilgwang-ro, Ilgwang-myeon, Gijang-gun, Busan, 619-911, Republic of Korea.
authors.forename: Taek-Jeong
authors.initials: TJ
authors.lastname: Nam
mesh_terms: D020418:Algal Proteins / Q000737:chemistry; D000595:Amino Acid Sequence; D000975:Antioxidants / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D002482:Cellulose / Q000737:chemistry; D000460:Chlorophyta / Q000378:metabolism*; D056148:Chromatography, Reverse-Phase; D004591:Electrophoresis, Polyacrylamide Gel; D056945:Hep G2 Cells; D066248:High Fructose Corn Syrup / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D017665:Hydroxyl Radical / Q000737:chemistry; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry; D010851:Picrates / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry*; D017434:Protein Structure, Tertiary; D017382:Reactive Oxygen Species / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid; D013481:Superoxides
chemical_list: D020418:Algal Proteins; D000975:Antioxidants; D001713:Biphenyl Compounds; D066248:High Fructose Corn Syrup; D010851:Picrates; D010940:Plant Proteins; D017382:Reactive Oxygen Species; C052389:oxygen-evolving enhancer protein 1, plant; D013481:Superoxides; D017665:Hydroxyl Radical; D002482:Cellulose; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl; D010100:Oxygen
keywords: antioxidant protein; green seaweed; hydrogen peroxide scavenging activity; oxygen evolving enhancer protein