title: The rice transcription factor OsWRKY47 is a positive regulator of the response to water deficit stress.
abstract: OsWRKY47 is a divergent rice transcription factor belonging to the group II of the WRKY family. A transcriptomic analysis of the drought response of transgenic rice plants expressing P SARK ::IPT, validated by qPCR, indicated that OsWRKY47 expression was induced under drought stress in P SARK ::IPT plants. A PCR-assisted site selection assay (SELEX) of recombinant OsWRKY47 protein showed that the preferred sequence bound in vitro is (G/T)TTGACT. Bioinformatics analyses identified a number of gene targets of OsWRKY47; among these two genes encode a Calmodulin binding protein and a Cys-rich secretory protein. Using Oswrk47 knockout mutants and transgenic rice overexpressing OsWRKY47 we show that the transcription of these putative targets were regulated by OsWRKY47. Phenotypic analysis carried out with transgenic rice plants showed that Oswrky47 mutants displayed higher sensitivity to drought and reduced yield, while plants overexpressing OsWRKY47 were more tolerant.
authors.affiliation: Instituto de Agrobiotecnología del Litoral (CONICET-UNL), Colectora ruta 168 km 0, Paraje El Pozo, 3000, Santa Fe, Argentina.
authors.forename: Jesica
authors.initials: J
authors.lastname: Raineri
authors.forename: Songhu
authors.initials: S
authors.lastname: Wang
authors.forename: Zvi
authors.initials: Z
authors.lastname: Peleg
authors.forename: Eduardo
authors.initials: E
authors.lastname: Blumwald
authors.forename: Raquel Lia
authors.initials: RL
authors.lastname: Chan
mesh_terms: D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002148:Calmodulin-Binding Proteins / Q000235:genetics / Q000378:metabolism; D018744:DNA, Plant / Q000235:genetics / Q000378:metabolism; D055864:Droughts; D055786:Gene Knockout Techniques; D017343:Genes, Plant; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D002148:Calmodulin-Binding Proteins; D018744:DNA, Plant; D010940:Plant Proteins; D014157:Transcription Factors