title: Nutraceutical properties of the methanolic extract of edible mushroom Cantharellus cibarius (Fries): primary mechanisms.
abstract: The methanolic extract of the wild edible mushroom Cantharellus cibarius Fr. (chanterelle) was analyzed for in vitro antioxidative, cytotoxic, antihypertensive and antibacterial activities. Various primary and secondary metabolites were found. Phenols were the major antioxidant components found in the extract (49.8 mg g(-1)), followed by flavonoids, whose content was approximately 86% of the total phenol content. Antioxidant activity, measured by four different methods, was high for inhibition of lipid peroxidation (EC50 = 1.21 mg mL(-1)) and chelating ability (EC50 = 0.64 mg mL(-1)). The antioxidant activity of the C. cibarius methanol extract was achieved through chelating iron compared to hydrogen atom and/or electron transfer. The extract showed good selectivity in cytotoxicity on human cervix adenocarcinoma HeLa, breast carcinoma MDA-MB-453 and human myelogenous leukemia K562, compared to normal control human fetal lung fibroblasts MRC-5 and human lung bronchial epithelial cells BEAS-2B. The extract had inhibitory activity against angiotensin converting I enzyme (ACE) (IC50 = 0.063 mg mL(-1)). The extract revealed selective antimicrobial activity against Gram-positive bacteria with the highest potential against E. faecalis. The medicinal and health benefits, observed in wild C. cibarius mushroom, seem an additional reason for its traditional use as a popular delicacy food.
authors.affiliation: Department for Chemistry and Biochemistry, Faculty of Agriculture, University of Belgrade, Nemanjina 6, Belgrade 11080, Serbia.
authors.forename: Maja
authors.initials: M
authors.lastname: Kozarski
authors.forename: Anita
authors.initials: A
authors.lastname: Klaus
authors.forename: Jovana
authors.initials: J
authors.lastname: Vunduk
authors.forename: Zeljko
authors.initials: Z
authors.lastname: Zizak
authors.forename: Miomir
authors.initials: M
authors.lastname: Niksic
authors.forename: Dragica
authors.initials: D
authors.lastname: Jakovljevic
authors.forename: Miroslav M
authors.initials: MM
authors.lastname: Vrvic
authors.forename: Leo J L D
authors.initials: LJ
authors.lastname: Van Griensven
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000009:adverse effects / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000009:adverse effects / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D000970:Antineoplastic Agents / Q000009:adverse effects / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D000975:Antioxidants / Q000009:adverse effects / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D001487:Basidiomycota / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D001688:Biological Products / Q000009:adverse effects / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D002460:Cell Line; D045744:Cell Line, Tumor; D002470:Cell Survival; D019587:Dietary Supplements / Q000009:adverse effects / Q000032:analysis; D013293:Enterococcus faecalis / Q000254:growth & development / Q000378:metabolism; D005419:Flavonoids / Q000009:adverse effects / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D065928:Forests; D048690:Fruiting Bodies, Fungal / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism; D006801:Humans; D007502:Iron Chelating Agents / Q000009:adverse effects / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D015227:Lipid Peroxidation; D000432:Methanol / Q000737:chemistry; D054548:Montenegro; D010636:Phenols / Q000009:adverse effects / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D064209:Phytochemicals / Q000009:adverse effects / Q000032:analysis / Q000096:biosynthesis / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D000975:Antioxidants; D001688:Biological Products; D005419:Flavonoids; D007502:Iron Chelating Agents; D010636:Phenols; D064209:Phytochemicals; D012997:Solvents; D000432:Methanol