title: Genotoxic potential and heart rate disorders in the Mediterranean mussel Mytilus galloprovincialis exposed to Superdispersant-25 and dispersed diesel oil.
abstract: The effects of ex situ exposure of Mytilus galloprovincialis to Superdispersant-25 (S-25), diesel oil and dispersed diesel oil mixtures were studied by the impact on level of DNA damage in haemocytes (comet assay) and the cardiac activity patterns of mussels. Specimens were exposed for 72 h in a static system to diesel oil (100 μL/L and 1 mL/L), S-25 (5 and 50 μL/L), and dispersed diesel oil mixtures M1 (diesel oil 100 μL/L + S-25 5 μL/L) and M2 (diesel oil 1 mL/L + S-25 50 μL/L). For positive control 40 μM CdCl2 was used. The comet assay results indicated genotoxic potential of S-25 while the effects of diesel oil alone were not observed. The highest response was detected for M1 while the effects of M2 were not detected. The heart rate disorders were recorded for the diesel oil (1 mL/L), S-25 (50 μL/L) and both dispersed diesel oil mixtures.
authors.affiliation: Institute of Marine Biology - Kotor, University of Montenegro, Dobrota bb, 85330, Kotor, Montenegro.
authors.forename: Rajko
authors.initials: R
authors.lastname: Martinović
authors.affiliation: Center for Genotoxicology and Ecogenotoxicology, Faculty of Biology, Studentski trg 16, University of Belgrade, Belgrade, Serbia. Electronic address: stoimirk@bio.bg.ac.rs.
authors.forename: Stoimir
authors.initials: S
authors.lastname: Kolarević
authors.affiliation: Institute for Biological Research "Siniša Stanković", Despota Stefana 142, University of Belgrade, Belgrade, Serbia.
authors.forename: Margareta
authors.initials: M
authors.lastname: Kračun-Kolarević
authors.affiliation: Department of Natural Resources and Environmental Sciences, Institute for Multidisciplinary Research, Kneza Višeslava 1, University of Belgrade, Belgrade, Serbia.
authors.forename: Jovana
authors.initials: J
authors.lastname: Kostić
authors.affiliation: Institute of Marine Biology - Kotor, University of Montenegro, Dobrota bb, 85330, Kotor, Montenegro.
authors.forename: Sandra
authors.initials: S
authors.lastname: Marković
authors.affiliation: Department of Natural Resources and Environmental Sciences, Institute for Multidisciplinary Research, Kneza Višeslava 1, University of Belgrade, Belgrade, Serbia.
authors.forename: Zoran
authors.initials: Z
authors.lastname: Gačić
authors.affiliation: Institute of Marine Biology - Kotor, University of Montenegro, Dobrota bb, 85330, Kotor, Montenegro.
authors.forename: Zoran
authors.initials: Z
authors.lastname: Kljajić
authors.affiliation: Center for Genotoxicology and Ecogenotoxicology, Faculty of Biology, Studentski trg 16, University of Belgrade, Belgrade, Serbia.
authors.forename: Branka
authors.initials: B
authors.lastname: Vuković-Gačić
mesh_terms: D000818:Animals; D004247:DNA / Q000187:drug effects; D004249:DNA Damage; D004781:Environmental Exposure; D005742:Gasoline / Q000633:toxicity*; D006339:Heart Rate / Q000187:drug effects; D006434:Hemocytes / Q000187:drug effects; D009153:Mutagens / Q000633:toxicity*; D049878:Mytilus / Q000187:drug effects*; D013501:Surface-Active Agents / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D005742:Gasoline; D009153:Mutagens; D013501:Surface-Active Agents; D014874:Water Pollutants, Chemical; D004247:DNA
keywords: Cardiac activity pattern; Comet assay; Dispersed oil; Mytilus galloprovincialis; Superdispersant-25