title: Adlayer-mediated antibody immobilization to stainless steel for potential application to endothelial progenitor cell capture.
abstract: This work describes the straightforward surface modification of 316L stainless steel with BTS, S-(11-trichlorosilylundecanyl)-benzenethiosulfonate, a thiol-reactive trichlorosilane cross-linker molecule designed to form intermediary coatings with subsequent biofunctionalization capability. The strategy is more specifically exemplified with the immobilization of intact antibodies and their Fab' fragments. Both surface derivatization steps are thoroughly characterized by means of X-ray photoelectron spectroscopy. The antigen binding capability of both types of biofunctionalized surfaces is subsequently assessed by fluorescence microscopy. It was determined that BTS adlayers achieve robust immobilization of both intact and fragmented antibodies, while preserving antigen binding activity. Another key finding was the observation that the Fab' fragment immobilization strategy would constitute a preferential option over that involving intact antibodies in the context of in vivo capture of endothelial progenitor cells in stent applications.
authors.affiliation: †Department of Chemistry, University of Toronto, Toronto, Ontario M5S 3H6, Canada.
authors.forename: Pasquale
authors.initials: P
authors.lastname: Benvenuto
authors.affiliation: †Department of Chemistry, University of Toronto, Toronto, Ontario M5S 3H6, Canada.
authors.forename: Miguel A D
authors.initials: MA
authors.lastname: Neves
authors.affiliation: ‡Econous Systems Inc., Toronto, Ontario M5S 3H6, Canada.
authors.forename: Christophe
authors.initials: C
authors.lastname: Blaszykowski
authors.affiliation: §Clinical Biochemistry, St. Michael's Hospital, Toronto, Ontario M5B 1W8, Canada.
authors.forename: Alexander
authors.initials: A
authors.lastname: Romaschin
authors.affiliation: †Department of Chemistry, University of Toronto, Toronto, Ontario M5S 3H6, Canada.
authors.forename: Timothy
authors.initials: T
authors.lastname: Chung
authors.affiliation: †Department of Chemistry, University of Toronto, Toronto, Ontario M5S 3H6, Canada.
authors.forename: Sa Rang
authors.initials: SR
authors.lastname: Kim
authors.affiliation: †Department of Chemistry, University of Toronto, Toronto, Ontario M5S 3H6, Canada.|‡Econous Systems Inc., Toronto, Ontario M5S 3H6, Canada.
authors.forename: Michael
authors.initials: M
authors.lastname: Thompson
mesh_terms: D000906:Antibodies / Q000737:chemistry* / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D001557:Benzenesulfonates / Q000138:chemical synthesis / Q000737:chemistry*; D002469:Cell Separation / Q000379:methods*; D066026:Endothelial Progenitor Cells / Q000166:cytology*; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D056951:Photoelectron Spectroscopy; D012821:Silanes / Q000138:chemical synthesis / Q000737:chemistry*; D013193:Stainless Steel / Q000737:chemistry*; D013499:Surface Properties
chemical_list: C000604615:(11-trichlorosilylundecanyl)-benzenethiosulfonate; D000906:Antibodies; D001557:Benzenesulfonates; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D012821:Silanes; D013193:Stainless Steel