title: Repeated electroacupuncture in obese Zucker diabetic fatty rats: adiponectin and leptin in serum and adipose tissue.
abstract: Fasted, male, obese, Zucker, diabetic fatty rats aged 10-16 weeks were anesthetized with 1% halothane in nitrous oxide-oxygen (3:1) on alternate weekdays over 2 weeks. Group 1 (n = 4) did not receive electroacupuncture (controls); Group 2 (n = 4) received electroacupuncture using the Zhongwan and the Guanyuan acupoints; Group 3 (n = 4) received electroacupuncture using the bilateral Zusanli acupoints; Group 4 (n = 6) received neither halothane in nitrous oxide:oxygen nor electroacupuncture. At the end of study, animals were injected with sodium pentobarbitone (60 mg/mL, i.p.), and blood and white adipose tissue were collected. Analysis of variance and Duncan's tests showed that the mean leptin in serum was significantly lower and the adiponectin:leptin ratio was significantly higher in Group 2 than in Group 1 (p < 0.05); for Group 4, the serum leptin was significantly higher than it was for Groups 1-3 (p < 0.05), and the adiponectin:leptin ratio was significantly lower than it was for Group 2 (p < 0.05). Similar changes occurred for the leptin levels in the pelvic adipose tissue. In addition, for Group 2, the mean serum insulin: glucose ratio was significantly higher than it was for Group 1 (p < 0.05); for Group 4 the mean serum insulin and insulin: glucose ratio were significantly higher than they were for Groups 1 and 3 (p < 0.05), but not Group 2 (p > 0.05). No significant differences in the serum or the adipose-tissue measurements between Groups 1 and 3 were observed (p > 0.05).
authors.affiliation: Department of Anatomy, University of Otago, Dunedin, New Zealand. Electronic address: phil.peplow@stonebow.otago.ac.nz.
authors.forename: Philip V
authors.initials: PV
authors.lastname: Peplow
mesh_terms: D052242:Adiponectin / Q000097:blood*; D052436:Adipose Tissue, White / Q000737:chemistry; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000628:therapy*; D015671:Electroacupuncture / Q000379:methods*; D020738:Leptin / Q000097:blood*; D008297:Male; D009765:Obesity / Q000097:blood / Q000628:therapy*; D051381:Rats; D011924:Rats, Zucker
chemical_list: D052242:Adiponectin; D020738:Leptin
keywords: Zucker rat; adiponectin; diabetes; electroacupuncture; leptin; male