title: Engineering of a hybrid route to enhance shikimic acid production in Corynebacterium glutamicum.
abstract: OBJECTIVE
  To exploit the archaeal shikimic acid (SA) synthesis pathway toenhance SA production in Corynebacterium glutamicum.
  
  
  RESULTS
  Genetic cassettes were constructed that encoded a hybrid route for SA synthesis, which were composed of the archaeal 6-deoxy-5-ketofructose 1-phosphate pathway and the bacterial SA pathway. Corynebacterium glutamicum strains that expressed the hybrid route increased SA production by 57%. A recombinant strain of C. glutamicum that simultaneously overexpressed the hybrid route and its native SA pathway produced 4.7 gSA/l, while C. glutamicum with only the native SA pathway produced 3.7 gSA/l.
  
  
  CONCLUSIONS
  A new hybrid route for SA production was successfully constructed, that effectively increased SA production in C. glutamicum.
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beichen-Xilu No. 1, Chaoyang District, Beijing, 100101, People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Cheng-Ying
authors.initials: CY
authors.lastname: Jiang
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Liu
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.forename: Shuang-Jiang
authors.initials: SJ
authors.lastname: Liu
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D053898:Biosynthetic Pathways; D019757:Carbon-Oxygen Lyases / Q000235:genetics / Q000378:metabolism; D048230:Corynebacterium glutamicum / Q000235:genetics* / Q000378:metabolism; D005285:Fermentation; D060847:Metabolic Engineering / Q000379:methods; D012765:Shikimic Acid / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D012765:Shikimic Acid; D019757:Carbon-Oxygen Lyases; C021578:methylglyoxal synthase