title: A Single Point Mutation Creating a Furin Cleavage Site in the Spike Protein Renders Porcine Epidemic Diarrhea Coronavirus Trypsin Independent for Cell Entry and Fusion.
abstract: The emerging porcine epidemic diarrhea virus (PEDV) requires trypsin supplementation to activate its S protein for membrane fusion and virus propagation in cell culture. By substitution of a single amino acid in the S protein, we created a recombinant PEDV with an artificial furin protease cleavage site N terminal of the putative fusion peptide (PEDV-SFCS). PEDV-SFCS exhibited trypsin-independent cell-cell fusion and was able to replicate in culture cells independently of trypsin, though to low titer.
authors.affiliation: Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, People's Republic of China.
authors.forename: Wentao
authors.initials: W
authors.lastname: Li
authors.affiliation: Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
authors.forename: Oliver
authors.initials: O
authors.lastname: Wicht
authors.affiliation: Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
authors.forename: Frank J M
authors.initials: FJ
authors.lastname: van Kuppeveld
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, People's Republic of China.
authors.forename: Qigai
authors.initials: Q
authors.lastname: He
authors.affiliation: Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
authors.forename: Peter J M
authors.initials: PJ
authors.lastname: Rottier
authors.affiliation: Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands b.j.bosch@uu.nl.
authors.forename: Berend-Jan
authors.initials: BJ
authors.lastname: Bosch
mesh_terms: D020816:Amino Acid Motifs; D019943:Amino Acid Substitution; D000818:Animals; D018352:Coronavirus Infections / Q000201:enzymology / Q000662:veterinary* / Q000821:virology; D045683:Furin / Q000378:metabolism*; D017354:Point Mutation; D053485:Porcine epidemic diarrhea virus / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D011499:Protein Processing, Post-Translational; D064370:Spike Glycoprotein, Coronavirus / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D013552:Swine; D013553:Swine Diseases / Q000201:enzymology* / Q000821:virology; D014357:Trypsin / Q000378:metabolism*; D053586:Virus Internalization
chemical_list: D064370:Spike Glycoprotein, Coronavirus; D014357:Trypsin; D045683:Furin