title: High-resolution Crystal Structure of Dimeric VP40 From Sudan ebolavirus.
abstract: Ebolaviruses cause severe hemorrhagic fever. Central to the Ebola life cycle is the matrix protein VP40, which oligomerizes and drives viral budding. Here we present the crystal structure of the Sudan virus (SUDV) matrix protein. This structure is higher resolution (1.6 Å) than previously achievable. Despite differences in the protein purification, we find that it still forms a stable dimer in solution, as was noted for other Ebola VP40s. Although the N-terminal domain interface by which VP40 dimerizes is conserved between Ebola virus and SUDV, the C-terminal domain interface by which VP40 dimers may further assemble is significantly smaller in this SUDV assembly.
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease Beryllium, Bedford, Massachusetts.
authors.forename: Matthew C
authors.initials: MC
authors.lastname: Clifton
authors.affiliation: Department of Immunology and Microbial Science.
authors.forename: Jessica F
authors.initials: JF
authors.lastname: Bruhn
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease Beryllium, Bedford, Massachusetts.
authors.forename: Kateri
authors.initials: K
authors.lastname: Atkins
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease Beryllium, Bainbridge Island, Washington.
authors.forename: Terry L
authors.initials: TL
authors.lastname: Webb
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease Beryllium, Bainbridge Island, Washington.
authors.forename: Ruth O
authors.initials: RO
authors.lastname: Baydo
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease Beryllium, Bainbridge Island, Washington.
authors.forename: Amy
authors.initials: A
authors.lastname: Raymond
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease Beryllium, Bainbridge Island, Washington.
authors.forename: Donald D
authors.initials: DD
authors.lastname: Lorimer
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease Beryllium, Bainbridge Island, Washington.
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Edwards
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease Seattle Biomedical Research Institute.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Myler
authors.affiliation: Department of Immunology and Microbial Science The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California.
authors.forename: Erica Ollmann
authors.initials: EO
authors.lastname: Saphire
mesh_terms: D029043:Ebolavirus / Q000737:chemistry* / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D055503:Protein Multimerization / Q000502:physiology; D017434:Protein Structure, Tertiary / Q000502:physiology; D012996:Solutions / Q000737:chemistry; D013397:Sudan; D014763:Viral Matrix Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D012996:Solutions; C080981:VP40 protein, virus; D014763:Viral Matrix Proteins
keywords: Ebola virus; Sudan ebolavirus; VP40; matrix protein; virus budding