title: Aldehyde capture ligation for synthesis of native peptide bonds.
abstract: Chemoselective reactions for amide bond formation have transformed the ability to access synthetic proteins and other bioconjugates through ligation of fragments. In these ligations, amide bond formation is accelerated by transient enforcement of an intramolecular reaction between the carboxyl and the amine termini of two fragments. Building on this principle, we introduce an aldehyde capture ligation that parlays the high chemoselective reactivity of aldehydes and amines to enforce amide bond formation between amino acid residues and peptides that are difficult to ligate by existing technologies.
authors.affiliation: Department of Chemistry, New York University, 100 Washington Square East, New York, New York 10003, United States.
authors.forename: Monika
authors.initials: M
authors.lastname: Raj
authors.affiliation: Department of Chemistry, New York University, 100 Washington Square East, New York, New York 10003, United States.
authors.forename: Huabin
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Chemistry, New York University, 100 Washington Square East, New York, New York 10003, United States.
authors.forename: Sarah L
authors.initials: SL
authors.lastname: Blosser
authors.affiliation: Department of Chemistry, New York University, 100 Washington Square East, New York, New York 10003, United States.
authors.forename: Marc A
authors.initials: MA
authors.lastname: Vittoria
authors.affiliation: Department of Chemistry, New York University, 100 Washington Square East, New York, New York 10003, United States.
authors.forename: Paramjit S
authors.initials: PS
authors.lastname: Arora
mesh_terms: D000447:Aldehydes / Q000737:chemistry*; D000577:Amides / Q000138:chemical synthesis* / Q000737:chemistry; D000588:Amines / Q000737:chemistry; D015394:Molecular Structure; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D000447:Aldehydes; D000577:Amides; D000588:Amines; D010455:Peptides