title: Bacteria repelling on highly-ordered alumina-nanopore structures.
abstract: Bacteria introduce diseases and infections to humans by their adherence to biomaterials, such as implants and surgical tools. Cell desorption is an effective step to reduce such damage. Here, we report mechanisms of bacteria desorption. An alumina nanopore structure (ANS) with pore size of 35 nm, 55 nm, 70 nm, and 80 nm was used as substrate to grow Escherichia coli (E. coli) cells. A bacteria repelling experimental method was developed to quantitatively evaluate the area percentage of adherent bacterial cells that represent the nature of cell adhesion as well as desorption. Results showed that there were two crucial parameters: contact angle and contact area that affect the adhesion/desorption. The cells were found to be more easily repelled when the contact angle increased. The area percentage of adherent bacterial cells decreased with the decrease in the contact area of a cell on ANS. This means that cell accessibility on ANS depends on the contact area. This research reveals the effectiveness of the nanopored structures in repelling cells.
authors.affiliation: Department of Mechanical Engineering, Texas A&M University , College Station, Texas 77843-3123, USA.
authors.forename: Sunghan
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Materials Science and Engineering, Texas A&M University , College Station, Texas 77843-3123, USA.
authors.forename: Yan
authors.identifier: 0000-0002-8595-4481
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Microbial Pathogenesis and Immunology, Texas A&M Health Science Center , Bryan, TX 77807-3206, College Station, Texas 77843-3123, USA.
authors.forename: Jeffrey D
authors.initials: JD
authors.lastname: Cirillo
authors.affiliation: Department of Mechanical Engineering, Texas A&M University , College Station, Texas 77843-3123, USA.
authors.forename: Andreas A
authors.initials: AA
authors.lastname: Polycarpou
authors.forename: Hong
authors.initials: H
authors.lastname: Liang