title: Beyond the borders--Biomedical applications of non-linear Raman microscopy.
abstract: Raman spectroscopy offers great promise for label free imaging in biomedical applications. Its use, however, is hampered by the long integration times required and the presence of autofluorescence in many samples which outshines the Raman signals. In order to overcome these limitations, a variety of different non-linear Raman imaging techniques have been developed over the last decade. This review describes biomedical applications of these novel but already mature imaging techniques.
authors.affiliation: Department of Chemistry, University of Konstanz, 78457 Konstanz, Germany.
authors.forename: Martin Josef
authors.initials: MJ
authors.lastname: Winterhalder
authors.affiliation: Department of Chemistry, University of Konstanz, 78457 Konstanz, Germany. Electronic address: andreas.zumbusch@uni-konstanz.de.
authors.forename: Andreas
authors.initials: A
authors.lastname: Zumbusch
mesh_terms: D000818:Animals; D035843:Biomedical Research / Q000295:instrumentation / Q000379:methods*; D002468:Cell Physiological Phenomena / Q000502:physiology; D006801:Humans; D004364:Pharmaceutical Preparations / Q000032:analysis; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D004364:Pharmaceutical Preparations
keywords: Biomedical imaging; CARS; Non-linear Raman; Optical microsopcy; SRS