title: Targeted Intracellular Controlled Drug Delivery and Tumor Therapy through in Situ Forming Ag Nanogates on Mesoporous Silica Nanocontainers.
abstract: Targeting nanocontainers to the pathological zone and controlling release of their cargoes, in particular delivery of anticancer drugs to specific tumor cells in a targeted and controlled manner, remain the key challenges in drug delivery. This paper reports the development of a traceable and tumor-targeted intracellular drug release nanocontainer. The nanocontainer is obtained by in situ growth of silver nanoparticles (AgNPs) on the surfaces of mesoporous silica nanospheres (MSNs) using a DNA-templated process. Additionally, drug release from the nanopores is achieved by selective glutathione (GSH)-triggered dismantle of the AgNPs, and the concurrent fluorescence change allows real-time monitoring of drug release efficacy and facile visualization of in vivo delivery events. After being functionalized with sgc8 aptamer on the outer shell of the AgNPs, the targeted nanocontainers are delivered into acute lymphoblastic leukemia cells by aptamer-mediated recognition and endocytosis. Moreover, the GSH-responsive process presents an improvement in the cell-specific drug release and chemotherapeutic inhibition of tumor growth.
authors.affiliation: †State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China.|‡Department of Chemistry and Environmental Engineering, Hunan City University, Yiyang, 413000, P. R. China.
authors.forename: Changhui
authors.initials: C
authors.lastname: Liu
authors.affiliation: †State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zheng
authors.affiliation: †State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Deng
authors.affiliation: †State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Ma
authors.affiliation: †State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China.
authors.forename: Jishan
authors.initials: J
authors.lastname: Li
authors.affiliation: †State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China.
authors.forename: Yinhui
authors.initials: Y
authors.lastname: Li
authors.affiliation: †State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China.|§School of Chemistry and Biological Engineering, Changsha University of Science and Technology, Changsha, 410004, P. R. China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Yang
authors.affiliation: #The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, 410011, P. R. China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Yang
authors.affiliation: #The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, 410011, P. R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: †State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China.|§School of Chemistry and Biological Engineering, Changsha University of Science and Technology, Changsha, 410004, P. R. China.
authors.forename: Ronghua
authors.initials: R
authors.lastname: Yang
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000627:therapeutic use; D004317:Doxorubicin / Q000008:administration & dosage / Q000737:chemistry*; D016503:Drug Delivery Systems; D006367:HeLa Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D054118:Nanospheres / Q000627:therapeutic use; D049329:Nanostructures / Q000008:administration & dosage / Q000737:chemistry*; D016062:Porosity; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000188:drug therapy* / Q000473:pathology; D012822:Silicon Dioxide / Q000008:administration & dosage / Q000737:chemistry; D012834:Silver / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D012834:Silver; D012822:Silicon Dioxide; D004317:Doxorubicin
keywords: DNA; drug delivery; glutathione; mesoporous silica nanoparticle; silver sphere