title: Simultaneous determination of acetaminophen and dihydrocodeine in human plasma by UPLC-MS/MS: Its pharmacokinetic application.
abstract: An ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed and validated to determine acetaminophen (AAP) and dihydrocodeine (DHC) in human plasma simultaneously. Plasma samples were prepared using protein precipitation with acetonitrile, the two analytes and the internal standard midazolam were separated on an Acquity UPLC BEH C18 column and mass spectrometric analysis was performed using a QTrap5500 mass spectrometer coupled with an electro-spray ionization (ESI) source in the positive ion mode. The MRM transitions of m/z 151.2→110.0 and m/z 302.3→199.2 were used to quantify for AAP and DHC, respectively. The linearity of this method was found to be within the concentration range of 50-10000ng/mL for AAP, and 1-100ng/mL for DHC in human plasma, respectively. The lower limit of quantification (LLOQ) was 50ng/mL and 1ng/mL for AAP and DHC in human plasma, respectively. The relative standard deviations (RSD) of intra and inter precision were less than 10% for both AAP and DHC. The analysis time of per sample was 1.0min. The developed and validated method was successfully applied to a pharmacokinetic study of AAP (500mg) with DHC (20mg) capsule in Chinese healthy volunteers (N=20).
authors.affiliation: Medical College of Henan University of Science and Technology, Luoyang 471003, PR China.
authors.forename: Xiangjun
authors.initials: X
authors.lastname: Qiu
authors.affiliation: The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325027, PR China.
authors.forename: Dan
authors.initials: D
authors.lastname: Lou
authors.affiliation: Medical College of Henan University of Science and Technology, Luoyang 471003, PR China.
authors.forename: Ding
authors.initials: D
authors.lastname: Su
authors.affiliation: Medical College of Henan University of Science and Technology, Luoyang 471003, PR China.
authors.forename: Zebin
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Medical College of Henan University of Science and Technology, Luoyang 471003, PR China.
authors.forename: Pengtao
authors.initials: P
authors.lastname: Gao
authors.affiliation: The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, PR China. Electronic address: wzzhangnansheng@163.com.
authors.forename: Nan-sheng
authors.initials: NS
authors.lastname: Zhang
mesh_terms: D000082:Acetaminophen / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003061:Codeine / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics; D004355:Drug Stability; D006801:Humans; D016014:Linear Models; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000082:Acetaminophen; C014481:dihydrocodeine; D003061:Codeine
keywords: Acetaminophen; Dihydrocodeine; Human plasma; Pharmacokinetic; UPLC–MS/MS