title: Oleuropein and hydroxytyrosol protect from bisphenol A effects in livers and kidneys of lactating mother rats and their pups'.
abstract: Bisphenol A (BPA) is a chemical found in hard plastics and the coatings of food and drinks cans which can behave in a similar way to estrogen and other hormones in the human body. This study aimed to evaluate the significance of the treatment with oleuropein and hydroxytyrosol olive leaves rich extracts in reducing functional perturbations and oxidative stress arising from BPA treatment in livers and kidneys of lactating mother rats and their pups'. For this, four groups of lactating mothers were used: controls (group A), treated with bisphenol A (group B), treated with bisphenol A and oleuropein (group C) and with bisphenol A and hydroxytyrosol (group D). As results, we had found, in BPA treated group, either in mothers or in their pups', a significant decrease in morphological parameters, in catalase activity and in total antioxidant capacity associated to an increase in malondialdehyde levels in livers and kidneys. For these rats, the histological aspect showed, also, deep changes. Indeed, we had observed, in livers, hepatocellular necrosis associated to leucocytes infiltration and in kidneys tubular and glomerular necrosis. The co-treatments with BPA and oleuropein (group C) or with BPA and hydroxytyrosol (group D) ameliorate all morphological, biochemical and histological parameters as compared to BPA treated group B. The analysis of BPA and its derivatives with LC-MS/MS showed changes in their localizations between serum, livers or kidneys in all studied groups. In conclusion, the present study demonstrates the hepato-protective and reno-protective effects of oleuropein and hydroxytyrosol olive leaves extracts from BPA and its derivates toxicity.
authors.affiliation: Laboratory of Environmental Bioprocesses, Center of Biotechnology of Sfax, University of Sfax, PO Box 1177, 3038 Sfax, Tunisia.
authors.forename: Asma
authors.initials: A
authors.lastname: Mahmoudi
authors.affiliation: Laboratory of Environmental Bioprocesses, Center of Biotechnology of Sfax, University of Sfax, PO Box 1177, 3038 Sfax, Tunisia. Electronic address: ghorbelh1@yahoo.fr.
authors.forename: Héla
authors.initials: H
authors.lastname: Ghorbel
authors.affiliation: Laboratory of Environmental Bioprocesses, Center of Biotechnology of Sfax, University of Sfax, PO Box 1177, 3038 Sfax, Tunisia.
authors.forename: Zouhair
authors.initials: Z
authors.lastname: Bouallegui
authors.affiliation: Laboratory of Biochemistry, CHU Habib Bourguiba, 3029 Sfax, Tunisia.
authors.forename: Rim
authors.initials: R
authors.lastname: Marrekchi
authors.affiliation: Alliance for Research on North Africa (ARENA), Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8572, Ibaraki, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Isoda
authors.affiliation: Laboratory of Environmental Bioprocesses, Center of Biotechnology of Sfax, University of Sfax, PO Box 1177, 3038 Sfax, Tunisia.
authors.forename: Sami
authors.initials: S
authors.lastname: Sayadi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001559:Benzhydryl Compounds / Q000633:toxicity*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D057888:Iridoid Glucosides; D039823:Iridoids / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects; D007774:Lactation; D008099:Liver / Q000187:drug effects; D009035:Mothers; D018384:Oxidative Stress / Q000187:drug effects; D010636:Phenols / Q000633:toxicity*; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves; D051381:Rats; D053719:Tandem Mass Spectrometry; D000098826:Bisphenol A Compounds
chemical_list: D000975:Antioxidants; D001559:Benzhydryl Compounds; D057888:Iridoid Glucosides; D039823:Iridoids; D010636:Phenols; D010626:Phenylethyl Alcohol; D010936:Plant Extracts; C005975:3,4-dihydroxyphenylethanol; C002769:oleuropein; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A–bisphenol A derivates-oleuropein-hydroxytyrosol-lactating rats-liver-kidney