title: Evaluation of a modified sampling method for molecular analysis of air microflora.
abstract: A serious issue concerning the durability of economically important materials for humans related to cultural heritage is the process of biodeterioration. As a result of this phenomenon, priceless works of art, documents, and old prints have undergone a process of decomposition caused by microorganisms. Therefore, it is important to constantly monitor the presence and diversity of microorganisms in exposition rooms and storage areas of historical objects. In addition, the use of molecular biology tools for conservation studies will enable detailed research as well as reduce the time needed to perform the analyses compared with using conventional methods related to microbiology and conservation. The aim of this study was to adapt the sampling indoor air method for direct DNA extraction from microorganisms, including evaluating the extracted DNA quality and concentration. The obtained DNA was used to study the diversity of mold fungi in indoor air using polymerase chain reaction-denaturing gradient gel electrophoresis in specific archives and museum environments. The research was conducted in 2 storage rooms of the National Archives in Krakow and in 1 exposition room of the Archaeological Museum in Krakow (Poland).
authors.affiliation: Department of Microbiology, Faculty of Commodity Science, Cracow University of Economics, Rakowicka, Krakow, Poland lecht@uek.krakow.pl.
authors.forename: T
authors.initials: T
authors.lastname: Lech
authors.affiliation: Department of Environmental Biotechnology, Faculty of Power and Environmental Engineering, The Silesian University of Technology, Akademicka, Gliwice, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Ziembinska-Buczynska
mesh_terms: D000391:Air Microbiology; D001419:Bacteria / Q000235:genetics* / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004271:DNA, Fungal / Q000032:analysis / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis / Q000379:methods; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005658:Fungi / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods*; D009144:Museums; D011044:Poland; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results
chemical_list: D004269:DNA, Bacterial; D004271:DNA, Fungal