title: Determination of free and bound phenolic compounds in soy isoflavone concentrate using a PFP fused core column.
abstract: In the last years, the consumption of soy-based foods has increased due to the health benefits related to soy bioactives like phenolic compounds. Thus, in the present study, a new chromatographic method using reverse-phase high performance liquid chromatography coupled to diode array detection (RP-HPLC/DAD) was developed using a fused core pentafluorophenyl (PFP) column. The established method allowed the determination of twenty-one free phenolic compounds and eleven bound phenolics in a soy isoflavone concentrate. The method was validated in terms of precision and recovery. Intra and inter-day precision were less than 5% (% RSD) and the recovery was between 97.4% and 103.6%. Limits of quantification (LOQs) ranged between 0.093 and 0.443 μg/mL. Because of that, PFP stationary phase can be easily applied for routine determination of phenolic compounds in soy based foods.
authors.affiliation: Department of Chemistry and Physics (Analytical Chemistry Area), University of Almería, Carretera de Sacramento s/n, E-04120 Almería, Spain; Research Centre for Agricultural and Food Biotechnology (BITAL), Agrifood Campus of International Excellence, ceiA3, Carretera de Sacramento s/n, E-04120 Almería, Spain. Electronic address: vito.verardo@unibo.it.
authors.forename: Vito
authors.initials: V
authors.lastname: Verardo
authors.affiliation: Interdepartmental Centre of Agri-food Industrial Research (CIRI Agroalimentare), Alma Mater Studiorum - University of Bologna, Piazza Goidanich 60, I-47521 Cesena (FC), Italy.
authors.forename: Ylenia
authors.initials: Y
authors.lastname: Riciputi
authors.affiliation: Department of Chemistry and Physics (Analytical Chemistry Area), University of Almería, Carretera de Sacramento s/n, E-04120 Almería, Spain; Research Centre for Agricultural and Food Biotechnology (BITAL), Agrifood Campus of International Excellence, ceiA3, Carretera de Sacramento s/n, E-04120 Almería, Spain.
authors.forename: Antonia
authors.initials: A
authors.lastname: Garrido-Frenich
authors.affiliation: Interdepartmental Centre of Agri-food Industrial Research (CIRI Agroalimentare), Alma Mater Studiorum - University of Bologna, Piazza Goidanich 60, I-47521 Cesena (FC), Italy; Department of Agricultural and Food Sciences, Alma Mater Studiorum - University of Bologna, Piazza Goidanich 60, I-47521 Cesena (FC), Italy.
authors.forename: Maria Fiorenza
authors.initials: MF
authors.lastname: Caboni
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007529:Isoflavones / Q000032:analysis*; D057230:Limit of Detection; D010636:Phenols / Q000032:analysis*; D013025:Glycine max / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D007529:Isoflavones; D010636:Phenols
keywords: Fused core column; Isoflavones; PFP; Phenolic acids; Soybean