title: A gas chromatography-mass spectrometry based study on urine metabolomics in rats chronically poisoned with hydrogen sulfide.
abstract: Gas chromatography-mass spectrometry (GS-MS) in combination with multivariate statistical analysis was applied to explore the metabolic variability in urine of chronically hydrogen sulfide- (H2S-) poisoned rats relative to control ones. The changes in endogenous metabolites were studied by partial least squares-discriminate analysis (PLS-DA) and independent-samples t-test. The metabolic patterns of H2S-poisoned group are separated from the control, suggesting that the metabolic profiles of H2S-poisoned rats were markedly different from the controls. Moreover, compared to the control group, the level of alanine, d-ribose, tetradecanoic acid, L-aspartic acid, pentanedioic acid, cholesterol, acetate, and oleic acid in rat urine of the poisoning group decreased, while the level of glycine, d-mannose, arabinofuranose, and propanoic acid increased. These metabolites are related to amino acid metabolism as well as energy and lipid metabolism in vivo. Studying metabolomics using GC-MS allows for a comprehensive overview of the metabolism of the living body. This technique can be employed to decipher the mechanism of chronic H2S poisoning, thus promoting the use of metabolomics in clinical toxicology.
authors.affiliation: Analytical and Testing Center of Wenzhou Medical University, University-Town, Wenzhou 325035, China.
authors.forename: Mingjie
authors.initials: M
authors.lastname: Deng
authors.affiliation: Analytical and Testing Center of Wenzhou Medical University, University-Town, Wenzhou 325035, China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Analytical and Testing Center of Wenzhou Medical University, University-Town, Wenzhou 325035, China.
authors.forename: Fa
authors.initials: F
authors.lastname: Sun
authors.affiliation: Analytical and Testing Center of Wenzhou Medical University, University-Town, Wenzhou 325035, China.
authors.forename: Jianshe
authors.initials: J
authors.lastname: Ma
authors.affiliation: The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Lufeng
authors.initials: L
authors.lastname: Hu
authors.affiliation: The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Xuezhi
authors.initials: X
authors.lastname: Yang
authors.affiliation: The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Guanyang
authors.initials: G
authors.lastname: Lin
authors.affiliation: Analytical and Testing Center of Wenzhou Medical University, University-Town, Wenzhou 325035, China.
authors.forename: Xianqin
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry; D006862:Hydrogen Sulfide / Q000506:poisoning*; D055442:Metabolome / Q000187:drug effects*; D055432:Metabolomics; D011041:Poisoning / Q000652:urine*; D025341:Principal Component Analysis; D051381:Rats
chemical_list: D006862:Hydrogen Sulfide