title: Oxidized low-density lipoprotein alters endothelial progenitor cell populations.
abstract: Oxidized low-density lipoprotein (ox-LDL) is critical to atherosclerosis in hyperlipidemia. Bone marrow (BM)-derived endothelial progenitor cells (EPCs) are important to preventing atherosclerosis, and significantly decreased in hyperlipidemia. This study was to demonstrate ox-LDL and hyperlipidemia could exhibit similar effect on EPC population and the role of reactive oxygen species (ROS). ROS production in BM and blood was significantly increased in male C57BL/6 mice with intravenous ox-LDL treatment, and in hyperlipidemic LDL receptor knockout mice with 4-month high-fat diet. ROS formation was effectively blocked with overexpression of antioxidant enzymes or N-acetylcysteine treatment. In hyperlipidemic and ox-LDL-treated mice, c-Kit(+)/CD31(+) cell number in BM and blood, and Sca-1(+)/Flk-1(+) cell number in blood, not in BM, were significantly decreased, which were not affected by inhibiting ROS production, while blood CD34(+)/Flk-1(+) cell number was significantly increased that was prevented with reduced ROS formation. However, blood CD34(+)/CD133(+) cell number increased in ox-LDL-treated mice, while decreased in hyperlipidemic mice. These data suggested that ox-LDL produced significant changes in BM and blood EPC populations similar (but not identical) to chronic hyperlipidemia with predominantly ROS-independent mechanism(s).
authors.forename: Yuqi
authors.initials: Y
authors.lastname: Cui
authors.forename: Chandrakala A
authors.initials: CA
authors.lastname: Narasimhulu
authors.forename: Lingjuan
authors.initials: L
authors.lastname: Liu
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Yuan
authors.initials: Y
authors.lastname: Xiao
authors.forename: Jia
authors.initials: J
authors.lastname: Zhang
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Xie
authors.forename: Hong
authors.initials: H
authors.lastname: Hao
authors.forename: Jason Z
authors.initials: JZ
authors.lastname: Liu
authors.forename: Guanglong
authors.initials: G
authors.lastname: He
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Cowan
authors.forename: Lianqun
authors.initials: L
authors.lastname: Cui
authors.forename: Hua
authors.initials: H
authors.lastname: Zhu
authors.forename: Sampath
authors.initials: S
authors.lastname: Parthasarathy
authors.affiliation: Davis Heart and Lung Research Institute, The Ohio State University, Wexner Medical Center, Room 200 DHLRI; 473 West 12th Ave, Columbus, OH 43210, USA, zhenguo.liu@osumc.edu.
authors.forename: Zhenguo
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000378:metabolism; D002478:Cells, Cultured; D066026:Endothelial Progenitor Cells / Q000166:cytology* / Q000378:metabolism; D006949:Hyperlipidemias / Q000378:metabolism / Q000473:pathology; D008077:Lipoproteins, LDL / Q000378:metabolism* / Q000494:pharmacology; D008297:Male; D008810:Mice, Inbred C57BL; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D008077:Lipoproteins, LDL; D017382:Reactive Oxygen Species; C099252:oxidized low density lipoprotein