title: Experimental infection of inbred BALB/c and A/J mice with Massachusetts and Brazilian strains of infectious bronchitis virus (IBV).
abstract: The ability of avian coronaviruses to replicate in mice was investigated to investigate interspecies transmission. Two inbred mouse strains (BALB/c and A/J) with different genetic backgrounds were inoculated with the avian coronavirus strains Mass and BR-I and monitored for at least 10 days. Analysis of viral RNA, histopathological examinations, immunohistochemistry and serology were performed. After virus inoculation, neither clinical signs nor evident gross lesions were observed. Viral RNA, histopathological changes, and viral nucleoprotein were observed in the lung, trachea and sinus of all inoculated mice. Our study demonstrates the importance of elucidating the epidemiology of coronaviruses, including in rodents that are pests in poultry production.
authors.affiliation: Laboratory of Animal Virology, Institute of Biology, University of Campinas-UNICAMP, Campinas, 13083970, Brazil.
authors.forename: Matheus C
authors.initials: MC
authors.lastname: Martini
authors.forename: Jacy
authors.initials: J
authors.lastname: Gameiro
authors.forename: Tereza Cristina
authors.initials: TC
authors.lastname: Cardoso
authors.forename: Leonardo C
authors.initials: LC
authors.lastname: Caserta
authors.forename: Ana Carolina M
authors.initials: AC
authors.lastname: Gualberto
authors.forename: Lara Borges
authors.initials: LB
authors.lastname: Keid
authors.forename: Trícia M F de S
authors.initials: TM
authors.lastname: Oliveira
authors.forename: Marcia M A B
authors.initials: MM
authors.lastname: dos Santos
authors.forename: Clarice W
authors.initials: CW
authors.lastname: Arns
authors.forename: Helena L
authors.initials: HL
authors.lastname: Ferreira
mesh_terms: D000818:Animals; D001715:Bird Diseases / Q000235:genetics / Q000473:pathology / Q000821:virology; D018352:Coronavirus Infections / Q000235:genetics / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D004195:Disease Models, Animal; D001351:Infectious bronchitis virus / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D008168:Lung / Q000473:pathology / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D014132:Trachea / Q000473:pathology / Q000821:virology