title: High-resolution in vivo optical imaging of stroke injury and repair.
abstract: Central nervous system (CNS) function and dysfunction are best understood within a framework of interactions between neuronal, glial and vascular compartments comprising the neurovascular unit (NVU), all of which contribute to stroke-induced CNS injury, plasticity, repair, and recovery. Recent advances in in vivo optical microscopy have enabled us to observe and interrogate cells and their processes with high spatial resolution in real time and in their natural environment deep in the brain tissue. Here, we review some of these state-of-the-art imaging techniques with an emphasis on imaging the interactions among the constituents of the NVU during ischemic injury and repair in small animal models. This article is part of a Special Issue entitled SI: Cell Interactions In Stroke.
authors.affiliation: Optics Division, MHG/MIT/HMS Athinoula A Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USA. Electronic address: sava.sakadzic@mgh.harvard.edu.
authors.forename: Sava
authors.initials: S
authors.lastname: Sakadžić
authors.affiliation: Optics Division, MHG/MIT/HMS Athinoula A Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USA.
authors.forename: Jonghwan
authors.initials: J
authors.lastname: Lee
authors.affiliation: Optics Division, MHG/MIT/HMS Athinoula A Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USA.
authors.forename: David A
authors.initials: DA
authors.lastname: Boas
authors.affiliation: Neurovascular Research Laboratory, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USA; Stroke Service and Neuroscience Intensive Care Unit, Department of Neurology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA.
authors.forename: Cenk
authors.initials: C
authors.lastname: Ayata
mesh_terms: D000818:Animals; D001921:Brain / Q000098:blood supply / Q000473:pathology / Q000503:physiopathology*; D002450:Cell Communication / Q000502:physiology; D061848:Optical Imaging / Q000379:methods*; D020521:Stroke / Q000473:pathology / Q000503:physiopathology*
keywords: Cellular imaging; High-resolution; Multi-photon microscopy; Optical coherence tomography; Optical imaging; Photoacoustic imaging; Stroke