title: Evaluating Pediococcus acidilactici and Enterococcus faecium NRRL B-2354 as Thermal Surrogate Microorganisms for Salmonella for In-Plant Validation Studies of Low-Moisture Pet Food Products.
abstract: Pediococcus acidilactici ATCC 8042 and Enterococcus faecium NRRL B-2354 were investigated as potential surrogates for Salmonella serovars using thermal death time kinetics in products such as dry pet foods. The D-values of P. acidilactici ATCC 8042, E. faecium NRRL B-2354, and a cocktail of seven Salmonella serovars associated with low-moisture products were determined in a preservative-free dry pet food product at moisture levels of 9.1, 17.9, and 27.0% and heated between 76.7 and 87.8°C. The D-values were calculated by least squares linear regression. The D-values of P. acidilactici ATCC 8042 were higher than those for the Salmonella serovar cocktail but lower than those for E. faecium NRRL 2354. At 9.1% moisture, D-values of 6.54, 11.51, and 11.66 min at 76.7°C, 2.66, 3.22, and 4.08 min at 82.2°C, and 1.07, 1.29, and 1.69 min at 87.8°C were calculated for Salmonella serovars, P. acidilactici ATCC 8042, and E. faecium NRRL B-2354, respectively. The data suggest that the thermal inactivation characteristics of P. acidilactici ATCC 8042 can be utilized as a surrogate to predict the response of Salmonella in dry pet food products that are thermally processed at <90°C.
authors.affiliation: Silliker Inc., Food Science Center, 3600 Eagle Nest Drive, Crete, Illinois 60417, USA. Erdogan.Ceylan@silliker.com.
authors.forename: Erdogan
authors.initials: E
authors.lastname: Ceylan
authors.affiliation: Big Heart Pet Brands, 205 North Wiget Lane, Walnut Creek, California 94598, USA.
authors.forename: Derrick A
authors.initials: DA
authors.lastname: Bautista
mesh_terms: D000821:Animal Feed / Q000032:analysis / Q000382:microbiology*; D015169:Colony Count, Microbial; D016984:Enterococcus faecium / Q000737:chemistry / Q000254:growth & development*; D005506:Food Contamination / Q000032:analysis; D005511:Food Handling / Q000379:methods*; D006358:Hot Temperature; D007700:Kinetics; D010376:Pediococcus / Q000737:chemistry / Q000254:growth & development*; D012475:Salmonella / Q000737:chemistry / Q000254:growth & development*; D014867:Water / Q000032:analysis
chemical_list: D014867:Water