title: Reactive oxygen species generation by bovine blood neutrophils with different CXCR1 (IL8RA) genotype following Interleukin-8 incubation.
abstract: BACKGROUND
  Associations between polymorphisms in the bovine CXCR1 gene, encoding the chemokine (C-X-C motif) receptor 1 (IL8RA), and neutrophil traits and mastitis have been described. In the present study, blood neutrophils were isolated from 20 early lactating heifers with different CXCR1 genotype at position 735 or 980. The cells were incubated with different concentrations of recombinant bovine IL-8 (rbIL-8) for 2 or 6 h and stimulated with phorbol 12-myristate 13-acetate (PMA) or opsonized zymosan particles (OZP). Potential association between CXCR1 genotype and production of reactive oxygen species (ROS) was studied.
  
  
  RESULTS
  Although on single nucleotide polymorphisms (SNPs) may potentially affect CXCR1 function, SNPs c.735C > G and c.980A > G showed no association with ROS production with or without incubation of rbIL-8. Neutrophils incubated with rbIL-8 for 2 or 6 h showed higher PMA- and lower OZP-induced ROS production compared to control without rbIL-8.
  
  
  CONCLUSIONS
  In the present study no association could be detected between superoxide production by isolated bovine neutrophils during early lactation and CXCR1 gene polymorphism. IL-8 showed to possess inhibitory effects on ROS generation in bovine neutrophils.
authors.affiliation: M-team and Mastitis and Milk Quality Research Unit, Department of Reproduction, Obstetrics, and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan, 133, Merelbeke, Belgium. Joren.Verbeke@UGent.be.
authors.forename: Joren
authors.initials: J
authors.lastname: Verbeke
authors.affiliation: Department of Comparative Physiology and Biometrics, Faculty of Veterinary Medicine, Ghent University, Salisburylaan, 133, Merelbeke, Belgium. Xanthippe.Boulougouris@UGent.be.
authors.forename: Xanthippe
authors.initials: X
authors.lastname: Boulougouris
authors.affiliation: Department of Comparative Physiology and Biometrics, Faculty of Veterinary Medicine, Ghent University, Salisburylaan, 133, Merelbeke, Belgium. Carolien.Rogiers@UGent.be.
authors.forename: Carolien
authors.initials: C
authors.lastname: Rogiers
authors.affiliation: Department of Comparative Physiology and Biometrics, Faculty of Veterinary Medicine, Ghent University, Salisburylaan, 133, Merelbeke, Belgium. Chistian.Burvenich@UGent.be.
authors.forename: Christian
authors.initials: C
authors.lastname: Burvenich
authors.affiliation: Animal Genetics Laboratory, Department of Nutrition, Genetics, and Ethology, Faculty of Veterinary Medicine, Ghent University, Heidestraat, 19, Merelbeke, Belgium. Luc.Peelman@UGent.be.
authors.forename: Luc
authors.initials: L
authors.lastname: Peelman
authors.affiliation: Laboratory of Drug Quality & Registration, Department of Pharmaceutical Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg, 460, Ghent, Belgium. Bart.DeSpiegeleer@UGent.be.
authors.forename: Bart
authors.initials: B
authors.lastname: De Spiegeleer
authors.affiliation: M-team and Mastitis and Milk Quality Research Unit, Department of Reproduction, Obstetrics, and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan, 133, Merelbeke, Belgium. Sarne.DeVliegher@UGent.be.
authors.forename: Sarne
authors.initials: S
authors.lastname: De Vliegher
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D005260:Female; D005838:Genotype; D016209:Interleukin-8 / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D017382:Reactive Oxygen Species; D023062:Receptors, Interleukin-8A / Q000235:genetics / Q000378:metabolism*
chemical_list: D016209:Interleukin-8; D017382:Reactive Oxygen Species; D023062:Receptors, Interleukin-8A