title: Evaluation of Stem Cell Marker Expression in Canine B-Cell Lymphoma Cell Lines, B-Cell Lymphoma-generated Spheres and Primary Samples.
abstract: BACKGROUND
  Canine lymphoma has lately drawn focus as a model of human non-Hodgkin's lymphoma due to its spontaneous occurrence and similar biological behavior. Cells with stem cell-like characteristics are believed to play a key role in therapeutic failure. Thus, an initial characterization and the possibility of specific detection of such cells could bear significant value.
  
  
  METHODS
  Expression of 12 stem cell markers were analyzed in two canine B-cell lymphoma cell lines, their generated spheres, and in primary lymphoma samples by quantitative real-time polymerase chain reaction and partially by flow cytometry and immunocytochemistry.
  
  
  RESULTS
  Expression of maternal embryonic leucine zipper kinase (Melk) was significantly higher in CLBL-1, CLBL-1M and in primary B-cell lymphoma samples compared to non-neoplastic lymph nodes. Spheres displayed a higher expression of v-myc myelocytomatosis viral oncogene homolog (Myc) and lower expression of Cd44 compared to original cell lines and primary B-cell lymphoma samples.
  
  
  CONCLUSIONS
  The results suggest a potential interesting role of Melk in canine B-cell lymphoma. Furthermore, the up-regulation of Myc in serum-free-generated spheres offers interesting possibilities for functional assays characterizing the specific generated sub-population.
authors.affiliation: Small Animal Clinic, University of Veterinary Medicine Hannover, Hannover, Germany Division of Medicine, Clinic III, Hematology, Oncology and Palliative Medicine, University of Rostock, Rostock, Germany.
authors.forename: Wen
authors.initials: W
authors.lastname: Liu
authors.affiliation: Small Animal Clinic, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: Feyza
authors.initials: F
authors.lastname: Selçuk
authors.affiliation: Clinical Pathology, University of Veterinary Medicine Vienna, Vienna, Austria.
authors.forename: Barbara C
authors.initials: BC
authors.lastname: Rütgen
authors.affiliation: Small Animal Clinic, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Moulay
authors.affiliation: Small Animal Clinic, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: Saskia
authors.initials: S
authors.lastname: Willenbrock
authors.affiliation: Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine Vienna, Vienna, Austria.
authors.forename: Sabine E
authors.initials: SE
authors.lastname: Hammer
authors.affiliation: Small Animal Clinic, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: Katharina Anna
authors.initials: KA
authors.lastname: Sterenczak
authors.affiliation: Division of Medicine, Clinic III, Hematology, Oncology and Palliative Medicine, University of Rostock, Rostock, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Junghanss
authors.affiliation: Department of Pathology, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: Marion
authors.initials: M
authors.lastname: Hewicker-Trautwein
authors.affiliation: Small Animal Clinic, University of Veterinary Medicine Hannover, Hannover, Germany Ingo.nolte@tiho-hannover.de.
authors.forename: Ingo
authors.initials: I
authors.lastname: Nolte
authors.affiliation: Small Animal Clinic, University of Veterinary Medicine Hannover, Hannover, Germany Division of Medicine, Clinic III, Hematology, Oncology and Palliative Medicine, University of Rostock, Rostock, Germany.
authors.forename: Hugo
authors.initials: H
authors.lastname: Murua Escobar
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004285:Dogs; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D018960:Hyaluronan Receptors / Q000096:biosynthesis; D008198:Lymph Nodes / Q000473:pathology; D016393:Lymphoma, B-Cell / Q000235:genetics* / Q000473:pathology / Q000662:veterinary; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics* / Q000473:pathology; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology*; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis
chemical_list: D018960:Hyaluronan Receptors; D016271:Proto-Oncogene Proteins c-myc
keywords: Lymphoma; canine model; dog; spheres; stem cell marker