title: The hematopoietic stem cell number in the peripheral blood of pediatric recipients correlates with the outcome after living donor liver transplantation.
abstract: It has been proposed that circulating HSCs play a role in graft survival after liver transplantation. The aim was to analyze the relationship between the number of HSCs before and after LDLT and liver function, immune biomarkers, and clinical outcomes in pediatric patients. We studied 15 pairs of adult healthy liver donors and pediatric recipients with ESLD. The CD34/CD45+ cell number was measured in the blood via flow cytometry, and plasma levels of immune biomarkers - via ELISA. CD34/CD45+ cell number in the recipients decreased within the first week after LDLT. The cell number before LDLT was negatively correlated with the plasma levels of CRP and the development of graft dysfunction in the early post-transplant period. After LDLT, the CD34/CD45+ cell number was positively correlated with the pretransplant plasma level of sCD40L, a T-cell activation marker. In adult liver donors, the cell number did not change within the first week after liver resection and was lower than in pediatric recipients. The results suggest that in pediatric recipients, the HSC number may be associated with graft function and could be regarded as a potential predictor of the clinical outcome after LDLT.
authors.affiliation: V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Moscow, Russian Federation.|Department of Transplantology and Artificial Organs, I.M. Sechenov Fist Moscow State Medical University, Moscow, Russian Federation.
authors.forename: Sergey V
authors.initials: SV
authors.lastname: Gautier
authors.affiliation: V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Moscow, Russian Federation.|Department of Transplantology and Artificial Organs, I.M. Sechenov Fist Moscow State Medical University, Moscow, Russian Federation.
authors.forename: Olga P
authors.initials: OP
authors.lastname: Shevchenko
authors.affiliation: V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Moscow, Russian Federation.
authors.forename: Olga M
authors.initials: OM
authors.lastname: Tsirulnikova
authors.affiliation: V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Moscow, Russian Federation.
authors.forename: Rivada M
authors.initials: RM
authors.lastname: Kurabekova
authors.affiliation: Russian Medical Academy of Postgraduate Education, Moscow, Russian Federation.
authors.forename: Svetlana A
authors.initials: SA
authors.lastname: Lugovskaya
authors.affiliation: Russian Medical Academy of Postgraduate Education, Moscow, Russian Federation.
authors.forename: Elena V
authors.initials: EV
authors.lastname: Naumova
authors.affiliation: V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Moscow, Russian Federation.
authors.forename: Irina E
authors.initials: IE
authors.lastname: Tsirulnikova
authors.affiliation: Russian Medical Academy of Postgraduate Education, Moscow, Russian Federation.
authors.forename: Vladimir V
authors.initials: VV
authors.lastname: Dolgov
mesh_terms: D016738:Alagille Syndrome / Q000097:blood / Q000601:surgery; D018952:Antigens, CD34 / Q000378:metabolism; D001656:Biliary Atresia / Q000097:blood / Q000601:surgery; D015415:Biomarkers / Q000097:blood; D001772:Blood Cell Count; D002675:Child, Preschool; D058625:End Stage Liver Disease / Q000097:blood / Q000601:surgery*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006085:Graft Survival; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007223:Infant; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008105:Liver Cirrhosis, Biliary / Q000097:blood / Q000601:surgery; D016031:Liver Transplantation / Q000009:adverse effects* / Q000379:methods*; D019520:Living Donors; D008297:Male; D016896:Treatment Outcome
chemical_list: D018952:Antigens, CD34; D015415:Biomarkers; D017493:Leukocyte Common Antigens
keywords: biliary; biomarkers; cirrhosis; graft function; hematopoietic stem cells; pediatric liver transplantation