title: Effects of canola proteins and hydrolysates on adipogenic differentiation of C3H10T/2 mesenchymal stem cells.
abstract: This study assessed the ability of canola protein isolate (CPI) and enzymatic hydrolysates (CPHs) to inhibit adipogenic differentiation of C3H10T1/2 murine mesenchymal stem cells in vitro. Cell viability was maintained at concentrations of 60 μg/ml of sample. Cells treated with Alcalase hydrolysate demonstrated a higher reduction in anti-adipogenic differentiation through quantitation by oil-red O staining. qPCR analysis showed that CPI and CPH-treated cells significantly inhibited PPARγ expression, a key transcription factor involved in adipocyte differentiation, as evident in an ∼ 60-80% fold reduction of PPARγ mRNA. Immunofluorescence staining for PPARγ protein also showed a reduced expression in some treated cells when compared to differentiated untreated cells. The 50% inhibition concentration (IC50) of CPI, CPHs and their membrane ultrafiltration fractions on pancreatic lipase (PL) activity ranged between 0.75 and 2.5 mg/ml, (p < 0.05) for the hydrolysed and unhydrolysed samples. These findings demonstrate that CPI and CPHs contain bioactive components which can modulate in vitro adipocyte differentiation.
authors.affiliation: Graham Centre for Agricultural Innovation, Charles Sturt University, Locked Bag 588, Wagga Wagga, NSW 2678, Australia; School of Agricultural and Wine Sciences, Charles Sturt University, Locked Bag 588, Wagga Wagga, NSW 2678, Australia. Electronic address: aalashi@csu.edu.au.
authors.forename: Adeola M
authors.initials: AM
authors.lastname: Alashi
authors.affiliation: Graham Centre for Agricultural Innovation, Charles Sturt University, Locked Bag 588, Wagga Wagga, NSW 2678, Australia; School of Biomedical Sciences, Charles Sturt University, Locked Bag 588, Wagga Wagga, NSW 2678, Australia.
authors.forename: Christopher L
authors.initials: CL
authors.lastname: Blanchard
authors.affiliation: Graham Centre for Agricultural Innovation, Charles Sturt University, Locked Bag 588, Wagga Wagga, NSW 2678, Australia.
authors.forename: Rodney J
authors.initials: RJ
authors.lastname: Mailer
authors.affiliation: Graham Centre for Agricultural Innovation, Charles Sturt University, Locked Bag 588, Wagga Wagga, NSW 2678, Australia; School of Agricultural and Wine Sciences, Charles Sturt University, Locked Bag 588, Wagga Wagga, NSW 2678, Australia.
authors.forename: Samson O
authors.initials: SO
authors.lastname: Agboola
authors.affiliation: Graham Centre for Agricultural Innovation, Charles Sturt University, Locked Bag 588, Wagga Wagga, NSW 2678, Australia; School of Agricultural and Wine Sciences, Charles Sturt University, Locked Bag 588, Wagga Wagga, NSW 2678, Australia.
authors.forename: A John
authors.initials: AJ
authors.lastname: Mawson
authors.affiliation: Department of Human Nutritional Sciences and the Richardson Centre for Functional Foods and Nutraceuticals, University of Manitoba, Winnipeg R3T 2N2, Canada.
authors.forename: Rotimi E
authors.initials: RE
authors.lastname: Aluko
authors.affiliation: Graham Centre for Agricultural Innovation, Charles Sturt University, Locked Bag 588, Wagga Wagga, NSW 2678, Australia; School of Biomedical Sciences, Charles Sturt University, Locked Bag 588, Wagga Wagga, NSW 2678, Australia.
authors.forename: Padraig
authors.initials: P
authors.lastname: Strappe
mesh_terms: D050156:Adipogenesis / Q000187:drug effects; D000818:Animals; D029688:Brassica napus / Q000737:chemistry*; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D008049:Lipase / Q000037:antagonists & inhibitors; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D047495:PPAR gamma / Q000032:analysis / Q000235:genetics; D010940:Plant Proteins / Q000494:pharmacology*; D011492:Protein Hydrolysates / Q000494:pharmacology*
chemical_list: D047495:PPAR gamma; D010940:Plant Proteins; D011492:Protein Hydrolysates; D008049:Lipase
keywords: Adipocyte differentiation; C3H10T1/2 mesenchymal stem cells; Canola protein; PPAR gamma; Pancreatic lipase; Protein hydrolysates