title: Xylan-Degrading Catalytic Flagellar Nanorods.
abstract: Flagellin, the main component of flagellar filaments, is a protein possessing polymerization ability. In this work, a novel fusion construct of xylanase A from B. subtilis and Salmonella flagellin was created which is applicable to build xylan-degrading catalytic nanorods of high stability. The FliC-XynA chimera when overexpressed in a flagellin deficient Salmonella host strain was secreted into the culture medium by the flagellum-specific export machinery allowing easy purification. Filamentous assemblies displaying high surface density of catalytic sites were produced by ammonium sulfate-induced polymerization. FliC-XynA nanorods were resistant to proteolytic degradation and preserved their enzymatic activity for a long period of time. Furnishing enzymes with self-assembling ability to build catalytic nanorods offers a promising alternative approach to enzyme immobilization onto nanostructured synthetic scaffolds.
authors.affiliation: Bio-Nanosystems Laboratory, Research Institute of Chemical and Process Engineering, Faculty of Information Technology, University of Pannonia, Egyetem u. 10, Veszprém, 8200, Hungary.
authors.forename: Ágnes
authors.initials: Á
authors.lastname: Klein
authors.forename: Veronika
authors.initials: V
authors.lastname: Szabó
authors.forename: Mátyás
authors.initials: M
authors.lastname: Kovács
authors.forename: Dániel
authors.initials: D
authors.lastname: Patkó
authors.forename: Balázs
authors.initials: B
authors.lastname: Tóth
authors.forename: Ferenc
authors.initials: F
authors.lastname: Vonderviszt
mesh_terms: D001412:Bacillus subtilis / Q000378:metabolism; D005408:Flagellin / Q000378:metabolism*; D043942:Nanotubes; D012475:Salmonella / Q000378:metabolism; D014990:Xylans / Q000378:metabolism*
chemical_list: D014990:Xylans; D005408:Flagellin