title: Interfacial force-driven pattern formation during drying of Aβ (25-35) fibrils.
abstract: Pattern formation during evaporation of biofluids finds significant applications in the biomedical field for disease identification. Aβ (25-35) is the smallest peptide in the amyloid peptide family that retains the toxicity of a full length peptide responsible for Alzheimer's disease and is chosen here as the model solute. Drying experiments on substrates of varying wettability exhibit unique drying patterns of Aβ (25-35) fibrils visualized through fluorescence microscopy and transmission electron microscopy. The unique pattern formations can be interpreted as manifestations of the changes in the self-pinning mechanism with changes in wettability, which in some cases resembles the well-known coffee ring effect. Additionally, the delicate balance between the drag and capillary forces has been perturbed by initiating controlled rates of evaporation and probing their effects on the fibril patterning.
authors.affiliation: Department of Chemical Engineering, Indian Institute of Technology Kharagpur, India.
authors.forename: Ayantika
authors.initials: A
authors.lastname: Sett
authors.affiliation: Department of Chemistry, Indian Institute of Technology Kharagpur, India.
authors.forename: Sudipta
authors.initials: S
authors.lastname: Bag
authors.affiliation: Department of Chemistry, Indian Institute of Technology Kharagpur, India. Electronic address: swagata@chem.iitkgp.ernet.in.
authors.forename: Swagata
authors.initials: S
authors.lastname: Dasgupta
authors.affiliation: Department of Chemical Engineering, Indian Institute of Technology Kharagpur, India. Electronic address: sunando@che.iitkgp.ernet.in.
authors.forename: Sunando
authors.initials: S
authors.lastname: DasGupta
mesh_terms: D000682:Amyloid / Q000737:chemistry / Q000648:ultrastructure*; D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000648:ultrastructure*; D052160:Benzothiazoles; D003890:Desiccation; D005456:Fluorescent Dyes; D006801:Humans; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D010446:Peptide Fragments / Q000737:chemistry / Q000648:ultrastructure*; D013844:Thiazoles; D017155:Wettability
chemical_list: D000682:Amyloid; D016229:Amyloid beta-Peptides; D052160:Benzothiazoles; D005456:Fluorescent Dyes; D010446:Peptide Fragments; D013844:Thiazoles; C113041:amyloid beta-protein (35-25); C009462:thioflavin T
keywords: Amyloid β (25–35) fibrils; Pattern formation; Wettability