title: Structure and gene cluster of the O-antigen of Escherichia coli O140.
abstract: An acidic O-polysaccharide (O-antigen) was isolated from the lipopolysaccharide of Escherichia coli O140 and studied by sugar analysis along with 1D and 2D (1)H and (13)C NMR spectroscopy. The following structure of the branched hexasaccharide repeating unit was established: [Formula: see text]. The O-antigen gene cluster of E. coli O140 was sequenced. The gene functions were tentatively assigned by a comparison with sequences in the available databases and found to be in full agreement with the E. coli O140 polysaccharide structure.
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation. Electronic address: perepel@ioc.ac.ru.
authors.forename: Andrei V
authors.initials: AV
authors.lastname: Perepelov
authors.affiliation: TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Wang
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors.forename: Sof'ya N
authors.initials: SN
authors.lastname: Senchenkova
authors.affiliation: TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Mei
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.affiliation: TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D005810:Multigene Family; D019081:O Antigens / Q000737:chemistry / Q000235:genetics*; D016366:Open Reading Frames
chemical_list: D019081:O Antigens
keywords: Bacterial polysaccharide structure; Escherichia coli; Lipopolysaccharide; O-antigen; O-antigen gene cluster