title: Genomic characterization of two novel polyomaviruses in Brazilian insectivorous bats.
abstract: Two novel genomes comprising ≈4.9 kb were identified by next-generation sequencing from pooled organs of Tadarida brasiliensis bats. The overall nucleotide sequence identities between the viral genomes characterized here were less than 80% in comparison to other polyomaviruses (PyVs), members of the family Polyomaviridae. The new genomes display the archetypal organization of PyVs, which includes open reading frames for the regulatory proteins small T antigen (STAg) and large T antigen (LTAg), as well as capsid proteins VP1, VP2 and VP3. In addition, an alternate ORF was identified in the early genome region that is conserved in a large monophyletic group of polyomaviruses. Phylogenetic analysis showed similar clustering with group of PyVs detected in Otomops and Chaerephon bats and some species of monkeys. In this study, the genomes of two novel PyVs were detected in bats of a single species, demonstrating that these mammals can harbor genetically diverse polyomaviruses.
authors.affiliation: Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Rua Sarmento Leite 500, Porto Alegre, Rio Grande do Sul, CEP 90050-170, Brazil, esmaile.sales@gmail.com.
authors.forename: Francisco Esmaile
authors.initials: FE
authors.lastname: de Sales Lima
authors.forename: Samuel Paulo
authors.initials: SP
authors.lastname: Cibulski
authors.forename: André Alberto
authors.initials: AA
authors.lastname: Witt
authors.forename: Ana Cláudia
authors.initials: AC
authors.lastname: Franco
authors.forename: Paulo Michel
authors.initials: PM
authors.lastname: Roehe
mesh_terms: D000818:Animals; D001938:Brazil; D002685:Chiroptera / Q000821:virology*; D016679:Genome, Viral; D023281:Genomics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D011120:Polyomavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D027601:Polyomavirus Infections / Q000662:veterinary* / Q000821:virology; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins