title: Surfactant mediated extraction of total phenolic contents (TPC) and antioxidants from fruits juices.
abstract: The aim of this study was to enhance the extraction of total phenolic contents (TPC) and antioxidants from fruit juices by the application of surfactants formulations instead of conventional solvents (methanol, ethanol and acetone). A variety of fruit infusions: apple red delicious (apple (rd)) (Malus domestica), Mcintosh apple (apple (i)) (Malus pumila), sweet lemon (Citrus limetta) and mango (Magnifera indica) were studied. Effect of water, organic solvents and five different aqueous surfactant formulations viz. SDS, Brij-35, Brij-58, Triton X-100 and Span-40 were explored for the extraction of TPC and determining the antioxidant activity (AA). The TPC and AA (%) were determined using Folin-Ciocalteu (FCA) and DPPH assay, respectively. The effect of surfactant type, concentration and common organic solvents on the extraction of TPC and AA (%) was studied using UV-visible spectrophotometric technique. Among all the extracting systems employed, Brij-58 showed the highest extraction efficiency.
authors.affiliation: Institute of Forensic Science & Criminology, Panjab University, Chandigarh 160 014, India. Electronic address: 25shweta@pu.ac.in.
authors.forename: Shweta
authors.initials: S
authors.lastname: Sharma
authors.affiliation: Institute of Forensic Science & Criminology, Panjab University, Chandigarh 160 014, India.
authors.forename: Shivpoojan
authors.initials: S
authors.lastname: Kori
authors.affiliation: Institute of Forensic Science & Criminology, Panjab University, Chandigarh 160 014, India.
authors.forename: Ankush
authors.initials: A
authors.lastname: Parmar
mesh_terms: D000975:Antioxidants / Q000032:analysis / Q000302:isolation & purification*; D001628:Beverages / Q000032:analysis*; D005638:Fruit / Q000737:chemistry*; D010636:Phenols / Q000032:analysis / Q000302:isolation & purification*; D011092:Polyethylene Glycols / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D010636:Phenols; D013501:Surface-Active Agents; D011092:Polyethylene Glycols; C515901:Brij 35
keywords: Antioxidant activity; DPPH; Extraction; FCA assay; Spectrophotometry; Surfactant; Total phenolic contents