title: Characterization of the distribution of rotational torque on electrorotation chips with 3D electrodes.
abstract: This is a study of in-plane and out-of-plane distribution of rotational torque (ROT-T) and effective electric field (EEF) on electrorotation (ER) devices with 3D electrodes using finite element modeling (FEM) and experimental method. The objective of this study is to investigate electrical characteristics of the ER devices with five different electrode geometries and obtain an optimum structure for ER experiments. Further, it provides a comparison between characteristics of the 3D electrodes and traditionally used 2D electrodes. 3D distributions of EEF were studied by the time-variant FEM. FEM results were verified experimentally by studying the rotation of biological cells. The results show that the variations of ROT-T and EEF over the measurement area of the devices are considerably large. This can potentially lead to misinterpretation of recorded data. Therefore, it is essential to specify the boundaries of the measurement area with minimum deviation from the central EEF. For this purpose, FE analyses were utilized to specify the optimal region. Thereby, with confining the measurements to these regions, the dependency of ROT-T on the spatial position of the particles can be eliminated. Comparisons have been made on the sustainability of the EEF and ROT-T distributions for each device, to find an optimum design. Analyses of the devices prove that utilization of the 3D electrodes eliminate irregularities of EEF and ROT-T along the z-axis. The Results show that triangular electrodes provide the highest sustainability for the in-plane ROT-T and EEF distribution, while the oblate elliptical and circular electrodes have the lowest variances along the z-axis.
authors.affiliation: Department of Electrical and Electronics Engineering, METU, Ankara, Turkey.|METU-MEMS Research and Applications Center, Ankara, Turkey.
authors.forename: Garsha
authors.initials: G
authors.lastname: Bahrieh
authors.affiliation: METU-MEMS Research and Applications Center, Ankara, Turkey.
authors.forename: Ebru
authors.initials: E
authors.lastname: Özgür
authors.affiliation: METU-MEMS Research and Applications Center, Ankara, Turkey.
authors.forename: Aziz
authors.initials: A
authors.lastname: Koyuncuoğlu
authors.affiliation: Department of Biology, METU, Ankara, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Erdem
authors.affiliation: Department of Biology, METU, Ankara, Turkey.
authors.forename: Ufuk
authors.initials: U
authors.lastname: Gündüz
authors.affiliation: Department of Electrical and Electronics Engineering, METU, Ankara, Turkey.|METU-MEMS Research and Applications Center, Ankara, Turkey.
authors.forename: Haluk
authors.initials: H
authors.lastname: Külah
mesh_terms: D003584:Cytological Techniques; D004566:Electrodes; D004594:Electrophysiology / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D020014:K562 Cells; D008962:Models, Theoretical; D012399:Rotation; D019415:Torque
keywords: 3D electrodes; Cell dielectric characterization; Electrorotation; Rotating electric field; Rotational torque