title: Obesity associated Lyplal1 gene is regulated in diet induced obesity but not required for adipocyte differentiation.
abstract: Obesity and its associated morbidities represent one of the major and most rapidly expanding health epidemics in the world. Recent genome-wide association studies (GWAS) have identified several variants in LYPLAL1 gene that are significantly associated with central obesity preferentially in females. However, the exact function of this gene in adipose tissue development and obesity remains completely uncharacterized. We found murine Lyplal1 gene demonstrated a depot and sex-specific expression profile in white adipose tissues (WAT), and was significantly reduced in the epididymal and retroperitoneal fats in a murine model of high fat diet induced obesity (DIO). Lyplal1 mRNA was mildly up-regulated during adipogenesis and enriched in mature adipocytes through a PPARγ-independent mechanism. However, overexpression and knockdown of Lyplal1 did not significantly perturb adipocyte differentiation, triacylglycerol accumulation and/or insulin signaling. These data highlight a depot-specific marked reduction of Lyplal1 transcripts in diet induced obesity but a dispensable role of Lyplal1 in adipose tissue development.
authors.affiliation: College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province 410128, China; Department of Physiology, Georgia Regents University, Augusta, GA 30912, USA.
authors.forename: Xinnuo
authors.initials: X
authors.lastname: Lei
authors.affiliation: Medical College of Georgia, Georgia Regents University, Augusta, GA 30912, USA.
authors.forename: Mayson
authors.initials: M
authors.lastname: Callaway
authors.affiliation: Department of Physiology, Georgia Regents University, Augusta, GA 30912, USA.
authors.forename: Hongyi
authors.initials: H
authors.lastname: Zhou
authors.affiliation: College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province 410128, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Physiology, Georgia Regents University, Augusta, GA 30912, USA. Electronic address: wechen@gru.edu.
authors.forename: Weiqin
authors.initials: W
authors.lastname: Chen
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism*; D050156:Adipogenesis / Q000235:genetics*; D052436:Adipose Tissue, White / Q000378:metabolism; D000818:Animals; D059305:Diet, High-Fat; D005260:Female; D007328:Insulin / Q000378:metabolism; D050152:Intra-Abdominal Fat / Q000378:metabolism; D008245:Lysophospholipase / Q000235:genetics* / Q000378:metabolism; D008297:Male; D051379:Mice; D009765:Obesity / Q000235:genetics* / Q000378:metabolism; D047495:PPAR gamma / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D015854:Up-Regulation
chemical_list: D007328:Insulin; D047495:PPAR gamma; D008245:Lysophospholipase; C561408:LYPLAL1 protein, human
keywords: Adipocyte differentiation; Fat distribution; Lyplal1; Obesity