title: Functional analysis of protein ubiquitination.
abstract: Alterations in the protein ubiquitination can lead to the development of serious pathological conditions and diseases and, therefore, are under extensive investigation. Here we detail the revised/updated version of two approaches for analyzing the functional activities of the ubiquitin transferring system and target protein ubiquitination. These approaches permit the analysis of protein ubiquitination within the cellular environment as well as in a tube when the purified components are used. The updates introduced in the protocols allow both to increase the sensitivity of the assays and to reduce the false positives often experienced in the analyses.
authors.affiliation: Laboratory of Molecular and Cellular Cancer Biology, Faculty of Medicine, Bar-Ilan University, Safed 1321504, Israel.
authors.forename: Gal
authors.initials: G
authors.lastname: Levy-Cohen
authors.affiliation: Laboratory of Molecular and Cellular Cancer Biology, Faculty of Medicine, Bar-Ilan University, Safed 1321504, Israel. Electronic address: michael.blank@biu.ac.il.
authors.forename: Michael
authors.initials: M
authors.lastname: Blank
mesh_terms: D001681:Biological Assay / Q000379:methods*; D057809:HEK293 Cells; D006801:Humans; D011506:Proteins / Q000378:metabolism*; D054875:Ubiquitination
chemical_list: D011506:Proteins
keywords: E3 ligases; Protein ubiquitination; Ubiquitin; Ubiquitin transferring system