title: The extracellular protease stl functions to inhibit migration of v'ch1 sensory neuron during Drosophila embryogenesis.
abstract: Proper migration of cells through the dense and complex extracellular matrix (ECM) requires constant restructuring of the ECM to allow cells to move forward in a smooth manner. This restructuring can occur through the action of extracellular enzymes. Among these extracellular enzymes is the ADAMTS (A Disintegrin And Metalloprotease with ThromboSpondin repeats) family of secreted extracellular proteases. Drosophila stl encodes an ADAMTS protease expressed in and around the peripheral nervous system (PNS) during embryogenesis. The absence of stl displayed one specific neuron, the v'ch1 sensory neuron, migrating to its target sooner than in wild type. During normal development, the v'ch1 sensory neuron migrates dorsally at the same time it is extending an axon ventrally toward the CNS. Surprisingly, in the absence of stl, the v'ch1 neuron migrated further dorsally as compared to the wild type at stage 15, but did not migrate past its correct target at stage 16, suggesting a novel role for this extracellular protease in inhibiting migration of this neuron past a certain point.
authors.affiliation: Department of Biology, Franklin and Marshall College, P.O. Box 3003, Lancaster, PA 17604-3003, USA.
authors.forename: Tashi
authors.initials: T
authors.lastname: Lhamo
authors.affiliation: Department of Biology, Franklin and Marshall College, P.O. Box 3003, Lancaster, PA 17604-3003, USA. Electronic address: aismat@fandm.edu.
authors.forename: Afshan
authors.initials: A
authors.lastname: Ismat
mesh_terms: D051722:ADAM Proteins / Q000378:metabolism*; D000818:Animals; D001369:Axons / Q000378:metabolism / Q000502:physiology; D002465:Cell Movement / Q000502:physiology*; D004330:Drosophila / Q000196:embryology* / Q000378:metabolism / Q000502:physiology; D029721:Drosophila Proteins / Q000378:metabolism*; D047108:Embryonic Development / Q000502:physiology*; D005109:Extracellular Matrix / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism*; D017933:Peripheral Nervous System / Q000378:metabolism / Q000502:physiology; D011984:Sensory Receptor Cells / Q000378:metabolism / Q000502:physiology*
chemical_list: D029721:Drosophila Proteins; D010447:Peptide Hydrolases; D051722:ADAM Proteins; C547843:stl protein, Drosophila
keywords: ADAMTS; Cell migration; Drosophila; PNS; Sensory neurons; stl