title: Generation and Imaging of Transgenic Mice that Express G-CaMP7 under a Tetracycline Response Element.
abstract: The spatiotemporally controlled expression of G-CaMP fluorescent calcium indicator proteins can facilitate reliable imaging of brain circuit activity. Here, we generated a transgenic mouse line that expresses G-CaMP7 under a tetracycline response element. When crossed with a forebrain-specific tetracycline-controlled transactivator driver line, the mice expressed G-CaMP7 in defined cell populations in a tetracycline-controlled manner, notably in pyramidal neurons in layer 2/3 of the cortex and in the CA1 area of the hippocampus; this expression allowed for imaging of the in vivo activity of these circuits. This mouse line thus provides a useful genetic tool for controlled G-CaMP expression in vivo.
authors.affiliation: RIKEN Brain Science Institute, Wako, Saitama, Japan; PRESTO, Japan Science and Technology Agency, Kawaguchi, Saitama, Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Sato
authors.affiliation: RIKEN Brain Science Institute, Wako, Saitama, Japan.
authors.forename: Masako
authors.initials: M
authors.lastname: Kawano
authors.affiliation: Brain Science Institute, Saitama University, Saitama, Japan.
authors.forename: Masamichi
authors.initials: M
authors.lastname: Ohkura
authors.affiliation: Brain Science Institute, Saitama University, Saitama, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Gengyo-Ando
authors.affiliation: Brain Science Institute, Saitama University, Saitama, Japan.
authors.forename: Junichi
authors.initials: J
authors.lastname: Nakai
authors.affiliation: RIKEN Brain Science Institute, Wako, Saitama, Japan; Brain Science Institute, Saitama University, Saitama, Japan.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Hayashi
mesh_terms: D000818:Animals; D056547:CA1 Region, Hippocampal / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D003952:Diagnostic Imaging / Q000379:methods*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D015870:Gene Expression / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence / Q000379:methods; D017966:Pyramidal Cells / Q000378:metabolism*; D020218:Response Elements / Q000235:genetics*; D013752:Tetracycline / Q000494:pharmacology
chemical_list: D008565:Membrane Proteins; D049452:Green Fluorescent Proteins; D013752:Tetracycline; D002118:Calcium