title: Analysis of ROP signaling in the leaf epidermis of mutant tomato with low-energy ion beam.
abstract: The importance of the ROP small GTPase signaling pathway in the regulation of cellular polarity growth in eukaryotes has been thoroughly studied. In this study, we examined the LeROP small GTPase (related to Arabidopsis thaliana genome LeROP GTPase in tomato) signaling of cell polarity growth in the mutant (M-1) tomato. Interestingly, we detected expansive growth of epidermis cells in M-1, in which the leaves appeared slightly lobed shaped. However, we observed jigsaw puzzle shaped and deeply lobed shaped leaves in wild-type leaf epidermis cells. The t-test showed significant difference (P < 0.05). Based on previous studies of the AtROP gene in Arabidopsis leaf epidermis cells, we hypothesized that the growth of mutant M-1 tomato leaf epidermis cell is related to AtROP gene signal transmission. The results of reverse transcription-polymerase chain reaction showed the expression of LeROP2, LeROP4, and LeROP7 in M-1 mutants were stronger than in wild-type cells. At the flowering stage, LeROP2 GTPase showed no expression in wild-type cells, but was expressed in mutant cells. This study revealed a link between the low-energy ion beam and the ROP GTPase signaling pathway in tomato. In addition, the ROP gene changes analyzed suggest a new mechanism for mutations following low-energy ion beam implantation.
authors.affiliation: Henan Province Key Laboratory of Ion Beam Bioengineering, Zhengzhou University, Zhengzhou, China.
authors.forename: Q X
authors.initials: QX
authors.lastname: Liang
authors.affiliation: School of Life Sciences, Zhengzhou University, Zhengzhou, China liangqiuxia@zzu.edu.cn.
authors.forename: G Q
authors.initials: GQ
authors.lastname: Cao
authors.affiliation: Henan Province Key Laboratory of Ion Beam Bioengineering, Zhengzhou University, Zhengzhou, China.
authors.forename: S P
authors.initials: SP
authors.lastname: Zhao
authors.affiliation: Henan Province Key Laboratory of Ion Beam Bioengineering, Zhengzhou University, Zhengzhou, China.
authors.forename: Q C
authors.initials: QC
authors.lastname: Huang
authors.affiliation: Zhengzhou City Institute of Vegetable, Zhengzhou University, Zhengzhou, China.
authors.forename: F Q
authors.initials: FQ
authors.lastname: Ying
authors.affiliation: Center of Technology, China Tobacco Henan Industrial Limited Company, Zhengzhou, China.
authors.forename: W
authors.initials: W
authors.lastname: Chen
mesh_terms: D001483:Base Sequence; D019204:GTP-Binding Proteins / Q000378:metabolism*; D015870:Gene Expression; D018551:Solanum lycopersicum / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects; D010802:Phylogeny; D019441:Plant Epidermis / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects; D010940:Plant Proteins / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D015398:Signal Transduction
chemical_list: D010940:Plant Proteins; D019204:GTP-Binding Proteins