title: Downregulation of miR-22 acts as an unfavorable prognostic biomarker in osteosarcoma.
abstract: miRNA-22 (miR-22) has been showed to involve in a variety of cancers; however, the association between miR-22 expression level and the prognosis of osteosarcoma is also poorly unknown. Fifty-two patients with surgically resected paired osteosarcoma and non-neoplastic disease between 2008 and 2014 were involved in this study. Real-time PCR was performed to examine the expression level of miR-22 in osteosarcoma tissues and noncancerous bone tissues. Then the association between miR-22 expression and clinical-pathological parameters were further evaluated. Kaplan-Meier analysis and Cox proportional hazards regression models were explored to reveal the correlations of miR-22 expression with survival of patients. The results indicated that miR-22 was downregulated in osteosarcoma tissues in comparison with noncancerous bone tissues. In addition, there is statistically significance between miR-22 expression level and recurrence, metastasis, and chemotherapy response. The patients with lower miR-22 expression level had both poorer overall survival and disease-free survival. The multivariant analysis revealed that the miR-22 expression level and metastasis status are independent prognosis factors for osteosarcoma. In conclusion, miR-22 was downregulated in osteosarcoma and its expression level was correlated with a variety of important clinical-pathological parameters. Moreover, miR-22 may serve as a promising biomarker for predicting the prognosis of osteosarcoma.
authors.affiliation: Department of Joint Surgery, Hainan General Hospital, Haikou, 570311, Hainan, China.
authors.forename: Guangji
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Joint Surgery, Hainan General Hospital, Haikou, 570311, Hainan, China.
authors.forename: Ningjiang
authors.initials: N
authors.lastname: Shen
authors.affiliation: Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Cheng
authors.affiliation: Department of Joint Surgery, Hainan General Hospital, Haikou, 570311, Hainan, China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Lin
authors.affiliation: Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China. kanghua_li1953@yeah.net.
authors.forename: Kanghua
authors.initials: K
authors.lastname: Li
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000235:genetics*; D001859:Bone Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D001842:Bone and Bones / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D060787:Neoplasm Grading; D009364:Neoplasm Recurrence, Local / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D009367:Neoplasm Staging; D012516:Osteosarcoma / Q000235:genetics* / Q000401:mortality / Q000556:secondary; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015996:Survival Rate
chemical_list: D014408:Biomarkers, Tumor; C548375:MIRN22 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger
keywords: Biomarker; Osteosarcoma; miR-22