title: Magnetic resonance imaging of tumor with a self-traceable polymer conjugated with an antibody fragment.
abstract: A (13)C-enriched phosphorylcholine polymer ((13)C-PMPC) as a self-traceable MR (magnetic resonance) tag was conjugated with a fragment (scFv) of Herceptin, a clinical antibody against antigen Her2. When injected in model mice bearing Her2(+) (gastric) and Her2(-) (pancreatic) tumors, the antibody-tag conjugate (13)C-PMPC-scFv selectively accumulated in the Her2(+) tumor with a rapid build-up/decay (accumulation/clearance) profile and, with the use of the (1)H-(13)C double-resonance (heteronuclear correlation) technique, the Her2(+) gastric tumor was clearly MR imaged.
authors.affiliation: Advanced Biomedical Engineering Research Unit, Center for the Promotion of Interdisciplinary Education and Research, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
authors.forename: Hisatsugu
authors.initials: H
authors.lastname: Yamada
authors.affiliation: Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Hasegawa
authors.affiliation: Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Suzuki
authors.affiliation: Department of Systems Science, Graduate School of Informatics, Kyoto University, Yoshida-honmachi, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Hirohiko
authors.initials: H
authors.lastname: Imai
authors.affiliation: Department of Systems Science, Graduate School of Informatics, Kyoto University, Yoshida-honmachi, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Matsuda
authors.affiliation: Research and Educational Unit of Leaders for Integrated Medical System, Center for the Promotion of Interdisciplinary Education and Research, Kyoto University, Nishikyo-ku, Kyoto 615-8510, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Kimura
authors.affiliation: Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan; Division of Multidisciplinary Chemistry, Institute for Chemical Research, Kyoto University, Gokanosho, Uji, Kyoto 611-0011, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Toshimitsu
authors.affiliation: Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan. Electronic address: aoyama.yasuhiro.78z@st.kyoto-u.ac.jp.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Aoyama
authors.affiliation: Advanced Biomedical Engineering Research Unit, Center for the Promotion of Interdisciplinary Education and Research, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan; Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan. Electronic address: teruyuki@scl.kyoto-u.ac.jp.
authors.forename: Teruyuki
authors.initials: T
authors.lastname: Kondo
mesh_terms: D000818:Animals; D002247:Carbon Isotopes / Q000493:pharmacokinetics; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D018796:Immunoconjugates / Q000737:chemistry / Q000493:pharmacokinetics*; D008279:Magnetic Resonance Imaging; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010190:Pancreatic Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D010767:Phosphorylcholine / Q000031:analogs & derivatives / Q000493:pharmacokinetics; D011109:Polymethacrylic Acids / Q000493:pharmacokinetics; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000276:immunology / Q000378:metabolism; D057127:Single-Chain Antibodies / Q000493:pharmacokinetics; D013274:Stomach Neoplasms / Q000175:diagnosis* / Q000378:metabolism
chemical_list: D002247:Carbon Isotopes; D018796:Immunoconjugates; D010767:Phosphorylcholine; D011109:Polymethacrylic Acids; D018719:Erb-b2 Receptor Tyrosine Kinases; D057127:Single-Chain Antibodies; C115766:poly(2-methacryloyloxyethyl-phosphorylcholine); C508053:ERBB2 protein, human
keywords: Active targeting; Anti-Her2 antibody; MRI; Self-traceable polymer; Tumor imaging