title: MiR-221 as a pre- and postoperative plasma biomarker for larynx cancer patients.
abstract: OBJECTIVE
  In order to identify a plasma microRNA (miRNA) signature of larynx cancer (LCa), we examined miRNAs profile of plasma samples obtained from 30 LCa patients (preoperative and postoperative serum samples) and 30 healthy controls.
  
  
  METHODS
  Basic science research study.
  
  
  METHODS
  MicroRNA profiling of eight plasma samples (four from preoperative, four from control individuals) were performed using miRNA microarray. Two of the significantly deregulated miRNAs were selected for further confirmation in the remaining samples using quantitative reverse-transcription polymerase chain reaction (qRT-PCR).
  
  
  RESULTS
  Microarray profiling and qRT-PCR analysis showed that miR-221 was upregulated in LCa plasma samples. Further qRT-PCR analysis demonstrated that miR-221 was at normal levels in postoperative plasma samples.
  
  
  CONCLUSIONS
  Plasma miR-221 may have a potential as a novel diagnostic/prognostic marker and might be considered as a therapeutic target in LCa.
  
  
  METHODS
  N/A.
authors.affiliation: Department of Medical Genetics, Istanbul University Cerrahpasa Medical School.
authors.forename: Seda Salman
authors.initials: SS
authors.lastname: Yilmaz
authors.affiliation: Department of Medical Genetics, Istanbul University Cerrahpasa Medical School.|Biruni University.
authors.forename: Esra
authors.initials: E
authors.lastname: Guzel
authors.affiliation: Department of Medical Genetics, Istanbul University Cerrahpasa Medical School.|Molecular Biology and Genetics Department, Erzurum Technical University (o.f.k.), Erzurum, Turkey.
authors.forename: Omer Faruk
authors.initials: OF
authors.lastname: Karatas
authors.affiliation: Department of Otorhinolaryngology, Cerrahpasa Medical School, Istanbul University, Istanbul.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Yilmaz
authors.affiliation: Department of Medicine and Dan L. Duncan Cancer Center Division of Biostatistics.
authors.forename: Chad J
authors.initials: CJ
authors.lastname: Creighton
authors.affiliation: Department of Medical Genetics, Istanbul University Cerrahpasa Medical School.|Biruni University.|Department of Pathology and Immunology, Baylor College of Medicine, Houston, Texas, U.S.A.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Ozen
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000097:blood* / Q000601:surgery; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D046228:Microarray Analysis; D008875:Middle Aged; D011184:Postoperative Period; D057234:Preoperative Period; D011379:Prognosis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; C525709:MIR221, human
keywords: Diagnostic and prognostic biomarkers; larynx cancer; miR-221; microRNA; plasma