title: Distribution of the ompA-types among ruminant and swine pneumonic strains of Pasteurella multocida exhibiting various cap-locus and toxA patterns.
abstract: Pasteurella multocida is an important pathogen in food-producing animals and numerous virulence genes have been identified in an attempt to elucidate the pathogenesis of pasteurellosis. Currently, some of these genes including the capsule biosynthesis genes, the toxA and the OMPs-encoding genes have been suggested as epidemiological markers. However, the number of studies concerning ruminant isolates is limited, while, no attempt has ever been made to investigate the existence of ompA sequence diversity among P. multocida isolates. The aim of the present study was the comparative analysis of 144 P. multocida pneumonic isolates obtained from sheep, goats, cattle and pigs by determining the distribution of the ompA-types in conjunction with the cap-locus and toxA patterns. The ompA genotypes of the isolates were determined using both a PCR-RFLP method and DNA sequence analysis. The most prevalent capsule biosynthesis gene among the isolates was capA (86.1%); a noticeable, however, rate of capD-positive isolates (38.6%) was found among the ovine isolates that had been associated primarily with the capsule type A in the past. Moreover, an unexpectedly high percentage of toxA-positive pneumonic isolates was noticed among small ruminants (93.2% and 85.7% in sheep and goats, respectively), indicating an important epidemiological role of toxigenic P. multocida for these species. Despite their great heterogeneity, certain ompA-genotypes were associated with specific host species, showing evidence of a host preference. The OmpA-based PCR-RFLP method developed proved to be a valuable tool in typing P. multocida strains.
authors.affiliation: Institute of Infectious and Parasitic Diseases of Thessaloniki, Ministry of Rural Development and Food, 54627 Thessaloniki, Greece.
authors.forename: C
authors.initials: C
authors.lastname: Vougidou
authors.affiliation: Laboratory of Clinical Bacteriology, Parasitology, Zoonoses, and Geographical Medicine, University of Crete, 71110 Crete, Greece; Regional Laboratory of Public Health (Crete), 71110 Crete, Greece.
authors.forename: V
authors.initials: V
authors.lastname: Sandalakis
authors.affiliation: Laboratory of Clinical Bacteriology, Parasitology, Zoonoses, and Geographical Medicine, University of Crete, 71110 Crete, Greece.
authors.forename: A
authors.initials: A
authors.lastname: Psaroulaki
authors.affiliation: Laboratory of Microbiology and Infectious Diseases, Veterinary School, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
authors.forename: V
authors.initials: V
authors.lastname: Siarkou
authors.affiliation: Laboratory of Microbiology and Infectious Diseases, Veterinary School, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
authors.forename: E
authors.initials: E
authors.lastname: Petridou
authors.affiliation: Veterinary Research Institute of Thessaloniki, Hellenic Agricultural Organisation-Demeter (NAGREF), Campus of Thermi, 57001 Thessaloniki, Greece. Electronic address: ekateriniadou@vri.gr.
authors.forename: L
authors.initials: L
authors.lastname: Ekateriniadou
mesh_terms: D000818:Animals; D016667:Bacterial Capsules / Q000235:genetics; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001427:Bacterial Toxins / Q000235:genetics; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D014644:Genetic Variation; D005838:Genotype; D015511:Goat Diseases / Q000382:microbiology; D006041:Goats; D008969:Molecular Sequence Data; D010326:Pasteurella Infections / Q000382:microbiology / Q000662:veterinary*; D016979:Pasteurella multocida / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology; D034561:Sheep, Domestic; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001427:Bacterial Toxins; D004269:DNA, Bacterial; C078167:OMPA outer membrane proteins
keywords: PCR-RFLP; Pasteurella multocida; Sequence analysis; ompA; toxA