title: A Toolkit of CRISPR-Based Genome Editing Systems in Drosophila.
abstract: The last couple of years have witnessed an explosion in development of CRISPR-based genome editing technologies in cell lines as well as in model organisms. In this review, we focus on the applications of this popular system in Drosophila. We discuss the effectiveness of the CRISPR/Cas9 systems in terms of delivery, mutagenesis detection, parameters affecting efficiency, and off-target issues, with an emphasis on how to apply this powerful tool to characterize gene functions.
authors.affiliation: Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China; Tsinghua Fly Center, Tsinghua University, Beijing 100084, China; School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China; College of Bioengineering, Hubei University of Technology, Wuhan 430068, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Xu
authors.affiliation: Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China.
authors.forename: Xingjie
authors.initials: X
authors.lastname: Ren
authors.affiliation: Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Sun
authors.affiliation: Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Wang
authors.affiliation: Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China.
authors.forename: Huan-Huan
authors.initials: HH
authors.lastname: Qiao
authors.affiliation: Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China.
authors.forename: Bo-Wen
authors.initials: BW
authors.lastname: Xu
authors.affiliation: Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China; Tsinghua Fly Center, Tsinghua University, Beijing 100084, China.
authors.forename: Lu-Ping
authors.initials: LP
authors.lastname: Liu
authors.affiliation: Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China. Electronic address: nijq@mail.tsinghua.edu.cn.
authors.forename: Jian-Quan
authors.initials: JQ
authors.lastname: Ni
mesh_terms: D000818:Animals; D064130:CRISPR-Associated Proteins / Q000235:genetics*; D064113:CRISPR-Cas Systems / Q000235:genetics*; D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics*; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics*; D000072669:Gene Editing / Q000379:methods*; D018390:Gene Targeting / Q000379:methods*; D049750:Genome, Insect / Q000235:genetics*; D017393:RNA Editing
chemical_list: D064130:CRISPR-Associated Proteins; D029721:Drosophila Proteins
keywords: CRISPR; Cas9; Drosophila; Genome editing; Off-target; sgRNA