title: Chemical Composition and Immunomodulatory Activity of Mycelia of the Hairy Bracket Mushroom, Trametes hirsuta (Higher Basidiomycetes).
abstract: Trametes hirsuta is a medicinal mushroom that produces laccase. Its mycelium is a by-product when this species is used for laccase production. Aiming to develop its potential medicinal value, we investigated the chemical composition and immunomodulatory activity of T. hirsuta mycelia (THM). Dried THM contained 26.06% protein, 1.15% fat, 57.87% carbohydrates, and 5.47% ash. Sixteen free amino acids (2.63% total content) and 6 5'-nucleotides (adenosine 5'-monophosphate, cytidine 5'-monophosphate, guanosine 5'-monophosphate, uridine 5'-monophosphate, xanthosine 5'-monophosphate, and inosine 5'-monophosphate) constituting 0.275% were detected. Dominant sugars and polyols were fructose (2.47%), mannitol (2.03%), and glucose (1.8%); trehalose and arabinose contents were less than 0.10%. Evaluation of immunomodulatory activity in mice showed that THM could improve macrophage phagocytic function and serum hemolysin concentrations, but only the low-dose group significantly enhanced the natural killer cell activity and increased the spleen index, and only the middle-dose group remarkably increased the thymus index. Therefore, T. hirsuta mycelia could enhance immune function in mice and have immunomodulatory activity.
authors.affiliation: Sericultural and Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences; Key Laboratory of Functional Foods, Ministry of Agriculture; Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou, China.
authors.forename: Rongxia
authors.initials: R
authors.lastname: Ma
authors.affiliation: Sericultural and Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences; Key Laboratory of Functional Foods, Ministry of Agriculture; Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou, China.
authors.forename: Rongling
authors.initials: R
authors.lastname: Yang
authors.affiliation: Sericultural and Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences; Key Laboratory of Functional Foods, Ministry of Agriculture; Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou, China.
authors.forename: Xueming
authors.initials: X
authors.lastname: Liu
authors.affiliation: Sericultural and Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences; Key Laboratory of Functional Foods, Ministry of Agriculture; Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou, China.
authors.forename: Zhiyi
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Sericultural and Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences; Key Laboratory of Functional Foods, Ministry of Agriculture; Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou, China.
authors.forename: Chunying
authors.initials: C
authors.lastname: Yang
authors.affiliation: Sericultural and Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences; Key Laboratory of Functional Foods, Ministry of Agriculture; Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou, China.
authors.forename: Siyuan
authors.initials: S
authors.lastname: Wang
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D002241:Carbohydrates / Q000737:chemistry; D006801:Humans; D007155:Immunologic Factors / Q000737:chemistry* / Q000494:pharmacology*; D007249:Inflammation / Q000188:drug therapy / Q000276:immunology; D008297:Male; D051379:Mice; D009000:Monocytes / Q000187:drug effects / Q000276:immunology; D025282:Mycelium / Q000737:chemistry; D009711:Nucleotides / Q000737:chemistry; D010587:Phagocytosis / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D055454:Trametes / Q000737:chemistry*; D014675:Vegetables / Q000737:chemistry
chemical_list: D000596:Amino Acids; D002241:Carbohydrates; D007155:Immunologic Factors; D009711:Nucleotides; D010936:Plant Extracts