title: Hypoxia-inducible factor-2α (HIF-2α) mediates the effects of hypoxia on the promotion of HeLa cell viability, colony formation, and invasion capacity in vitro.
abstract: Hypoxia reduces the oxygen supply to tumor cells and may limit tumor cell growth. However, hypoxia promotes tumor cell metabolic adaptation, apoptosis resistance, angiogenesis, invasion, and metastasis. Hypoxia-inducible factor-2α (HIF-2α) may be responsible for these hypoxia-induced changes. In this study, we investigated the effects of hypoxia and HIF-2α knockdown in HeLa cells. HIF-2α shRNA lentivirus was used to knock down HIF-2α expression; cell viability, colony formation, invasion capacity, and gene expression were assessed. Hypoxia promoted HeLa cell growth, whereas knockdown of HIF-2α expression reduced HeLa cell viability under both normoxic and hypoxic conditions, with a greater effect observed under hypoxic conditions. Knockdown of HIF-2α expression also reduced HeLa cell colony formation and invasion capacity under both normoxic and hypoxic conditions. Expression of cyclooxygenase 2 and vascular endothelial growth factor was reduced after knockdown of HIF-2α expression, with a greater effect observed under hypoxic conditions. HIF-2α mediated the hypoxia-induced effect on the promotion of HeLa cell viability, colony formation, and invasion capacity in vitro. Further studies are needed to confirm the in vivo relevance of hypoxia and HIF-2α.
authors.affiliation: Department of Gynecology and Obstetrics, The Second Hospital of Central South University, Changsha, Hunan, China xiongjing792004@aliyun.com.
authors.forename: J
authors.initials: J
authors.lastname: Xiong
authors.affiliation: Department of Gynecology and Obstetrics, The Second Hospital of Central South University, Changsha, Hunan, China.
authors.forename: F F
authors.initials: FF
authors.lastname: Zhu
authors.affiliation: Department of Gynecology and Obstetrics, The Second Hospital of Central South University, Changsha, Hunan, China.
authors.forename: M F
authors.initials: MF
authors.lastname: Nie
mesh_terms: D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics*; D015687:Cell Hypoxia; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D002470:Cell Survival / Q000235:genetics; D051546:Cyclooxygenase 2 / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D009361:Neoplasm Invasiveness; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014410:Tumor Stem Cell Assay / Q000379:methods*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics; D000099162:Endothelial PAS Domain-Containing Protein 1
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D051546:Cyclooxygenase 2; D042461:Vascular Endothelial Growth Factor A; D000099162:Endothelial PAS Domain-Containing Protein 1