title: Utilization of TALEN and CRISPR/Cas9 technologies for gene targeting and modification.
abstract: The capability to modify the genome precisely and efficiently offers an extremely useful tool for biomedical research. Recent developments in genome editing technologies such as transcription activator-like effector nuclease and the clustered regularly interspaced short palindromic repeats system have made genome modification available for a number of organisms with relative ease. Here, we introduce these genome editing techniques, compare and contrast each technical approach and discuss their potential to study the underlying mechanisms of human disease using patient-derived induced pluripotent stem cells.
authors.affiliation: Department of Physiology and Biophysics, State University of New York at Buffalo, Buffalo, New York 14214, USA Department of Neurology, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310009, China.
authors.forename: Jiali
authors.initials: J
authors.lastname: Pu
authors.affiliation: Department of Physiology and Biophysics, State University of New York at Buffalo, Buffalo, New York 14214, USA dafresca@buffalo.edu.
authors.forename: David
authors.initials: D
authors.lastname: Frescas
authors.affiliation: Department of Neurology, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310009, China.
authors.forename: Baorong
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Physiology and Biophysics, State University of New York at Buffalo, Buffalo, New York 14214, USA Veterans Affairs Western New York Healthcare System, Buffalo, New York 14215, USA.
authors.forename: Jian
authors.initials: J
authors.lastname: Feng
mesh_terms: D000818:Animals; D064112:Clustered Regularly Interspaced Short Palindromic Repeats; D018390:Gene Targeting / Q000379:methods*; D015894:Genome, Human; D006801:Humans; D057026:Induced Pluripotent Stem Cells
keywords: CRISPR/Cas9; TALENs; disease modeling; gene editing; gene therapy