title: Effect of dispersed crude oil on cardiac function in seabass Dicentrarchus labrax.
abstract: In this study, the impact of dispersed oil was assessed in Dicentrarchus labrax, a fish frequently used as an oil contamination indicator species. Fish were exposed for 48h to (mechanically and chemically) dispersed oil and dispersant alone. The impact of these exposure conditions was assessed on cardiac function by measuring (i) the contraction strength, the contraction and the relaxation speeds (ii) the cardiac energy metabolism using respirometry on permeabilized cardiac fibers. Compared to control, the increase of polycyclic aromatic metabolites observed in the bile indicated oil contamination in our fish. Following 48h of oil exposure at realistic oil concentrations, alterations of cardiac performances were observed. A decrease in contraction strength, contraction and relaxation speeds was observed in the presence of oil without effect of dispersant on these three parameters. Looking at cardiac energy metabolism, dispersant alone decreases all the activity of the respiratory chain and increases the proton leak. From these results, it appears that the observed decrease in cardiac performance in fish exposed to oil was not linked to a decrease in energy availability.
authors.affiliation: EA 4324 ORPHY (Optimisation des Régulations PHYsiologiques), UFR Sciences et Techniques, Université Européenne de Bretagne, Université de Brest, 6 avenue LE GORGEU, CS 93837, 29238 BREST cedex 3, France.
authors.forename: Florine
authors.initials: F
authors.lastname: Tissier
authors.affiliation: EA 4324 ORPHY (Optimisation des Régulations PHYsiologiques), UFR Sciences et Techniques, Université Européenne de Bretagne, Université de Brest, 6 avenue LE GORGEU, CS 93837, 29238 BREST cedex 3, France; Cedre: Centre de Documentation, de Recherche et d'Expérimentations sur les pollutions accidentelles des eaux, 15 rue Alain Colas, CS 41836, 29218 BREST Cedex 2, France. Electronic address: matthieudussauze@yahoo.fr.
authors.forename: Matthieu
authors.initials: M
authors.lastname: Dussauze
authors.affiliation: Cedre: Centre de Documentation, de Recherche et d'Expérimentations sur les pollutions accidentelles des eaux, 15 rue Alain Colas, CS 41836, 29218 BREST Cedex 2, France.
authors.forename: Nina
authors.initials: N
authors.lastname: Lefloch
authors.affiliation: EA 4324 ORPHY (Optimisation des Régulations PHYsiologiques), UFR Sciences et Techniques, Université Européenne de Bretagne, Université de Brest, 6 avenue LE GORGEU, CS 93837, 29238 BREST cedex 3, France.
authors.forename: Michael
authors.initials: M
authors.lastname: Theron
authors.affiliation: TOTAL Fluides S.A., 92907 Paris La défense, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Lemaire
authors.affiliation: Cedre: Centre de Documentation, de Recherche et d'Expérimentations sur les pollutions accidentelles des eaux, 15 rue Alain Colas, CS 41836, 29218 BREST Cedex 2, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Le Floch
authors.affiliation: EA 4324 ORPHY (Optimisation des Régulations PHYsiologiques), UFR Sciences et Techniques, Université Européenne de Bretagne, Université de Brest, 6 avenue LE GORGEU, CS 93837, 29238 BREST cedex 3, France.
authors.forename: Karine
authors.initials: K
authors.lastname: Pichavant-Rafini
mesh_terms: D000818:Animals; D001492:Bass; D001646:Bile / Q000378:metabolism; D004734:Energy Metabolism / Q000187:drug effects*; D006321:Heart / Q000187:drug effects*; D066298:In Vitro Techniques; D009200:Myocardial Contraction / Q000187:drug effects; D009206:Myocardium / Q000378:metabolism*; D009930:Organic Chemicals / Q000737:chemistry; D010578:Petroleum / Q000009:adverse effects*; D011084:Polycyclic Aromatic Hydrocarbons / Q000009:adverse effects*; D014874:Water Pollutants, Chemical / Q000009:adverse effects*
chemical_list: D009930:Organic Chemicals; D010578:Petroleum; D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; C048322:finasol OSR-5
keywords: Cardiac function; Dispersed oil; Seabass; Tissue respiration