title: Survival Rates with Time Course of Frozen-thawed Pacific Oyster Larvae in Indoor Rearing System.
abstract: Post-thawed larval rearing in Pacific oyster Crassostrea gigas was performed to investigate the survival rate with time course in three kinds of larvae cryopreserved. The highest survival rate and larval activity index (LAI) of post-thawed larvae were obtained from the permeation in 0.2 M sucrose and 2.0 M ethylene glycol (EG) at -1°C/min in freezing speed showing the survival rates just after thawing of 63.8% in trochophore, 84.1% in D-shaped veliger and 56.3% in early umbo veliger. In post-thawed larval rearing with food supply, the larvae lasted their lives until 24 hours in trochophore, 75 hours in D-shaped veliger and 57 hours in early umbo veliger. The results suggested that each larval stage post-thawed revealed no more further development to subsequent respective stage.
authors.affiliation: Department of Marine Bio-materials and Aquaculture, Pukyong National University, Busan 608-737, Republic of Korea.
authors.forename: Ki Tae
authors.initials: KT
authors.lastname: Kim
authors.affiliation: Department of Fisheries and Marine Resources, Mokpo National University, Jeonnam 534-830, Republic of Korea.
authors.forename: Han Kyu
authors.initials: HK
authors.lastname: Lim
authors.affiliation: Department of Marine Bio-materials and Aquaculture, Pukyong National University, Busan 608-737, Republic of Korea.
authors.forename: Young Jin
authors.initials: YJ
authors.lastname: Chang
keywords: Cryopreservation; Larvae; Pacific oyster; Post-thaw; Survival.