title: Accurate Detection of Hepatitis B Virus G1896A Mutant by Developed Taqman-ARMS Followed a Strict Control System.
abstract: BACKGROUND
  Hepatitis B virus (HBV) G1896A mutation was associated with HBeAg seronegativity and hepatitis B related acute-on-chronic liver failure. In this study, we developed Taqman amplification refractory mutation system (Taqman-ARMS) and established a strict control system to detect HBV G1896A mutant.
  
  
  METHODS
  HBV viral DNA was isolated from 60 patient serum samples, and full-length HBV genome was cloned. Then, Taqman-ARMS was used to detect HBV G1896A mutant.
  
  
  RESULTS
  The assay has the sensitivity of 1E+3 IU/ml G1896A template, and 0.1% weak population virus with G1896A could be found in mixtures. Total of all 60 clinical samples random collected were detected by Taqman-ARMS, the results were consistent with those by DNA sequencing.
  
  
  CONCLUSIONS
  The proposed Taqman-ARMS real-time PCR method for the detection of G1896A mutation of HBV was rapid, simple, sensitive, specific, and applicable in the clinical setting.
authors.affiliation: Department of Laboratory, The Third People's Hospital of Nantong, Nantong, Jiangsu, P. R., China.
authors.forename: Renfei
authors.initials: R
authors.lastname: Lu
authors.affiliation: Department of Laboratory, The Third People's Hospital of Nantong, Nantong, Jiangsu, P. R., China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Shao
authors.affiliation: Nantong Center for Disease Control and Prevention, Nantong, Jiangsu, P. R., China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Nantong Center for Disease Control and Prevention, Nantong, Jiangsu, P. R., China.
authors.forename: Hongping
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Laboratory, The Third People's Hospital of Nantong, Nantong, Jiangsu, P. R., China.
authors.forename: Yueping
authors.initials: Y
authors.lastname: Wu
mesh_terms: D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D009154:Mutation / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
keywords: G1896A mutation; HBV; Taqman-ARMS