title: Programmed Synthesis by Stimuli-Responsive DNAzyme-Modified Mesoporous SiO2 Nanoparticles.
abstract: DNAzyme-capped mesoporous SiO2 nanoparticles (MP SiO2 NPs) are applied as stimuli-responsive containers for programmed synthesis. Three types of MP SiO2 NPs are prepared by loading the NPs with Cy3-DBCO (DBCO=dibenzocyclooctyl), Cy5-N3 , and Cy7-N3 , and capping the NP containers with the Mg(2+) , Zn(2+) , and histidine-dependent DNAzyme sequences, respectively. In the presence of Mg(2+) and Zn(2+) ions as triggers, the respective DNAzyme-capped NPs are unlocked, leading to the "click" reaction product Cy3-Cy5. In turn, in the presence of Mg(2+) ions and histidine as triggers the second set of DNAzyme-capped NPs is unlocked leading to the Cy3-Cy7 conjugated product. The unloading of the respective NPs and the time-dependent formation of the products are followed by fluorescence spectroscopy (FRET). A detailed kinetic model for the formation of the different products is formulated and it correlates nicely with the experimental results.
authors.affiliation: The Hebrew University of Jerusalem, Institute of Chemistry, Center for Nanoscience and Nanotechnology, Jerusalem, 91904 (Israel).
authors.forename: Dora
authors.initials: D
authors.lastname: Balogh
authors.affiliation: The Hebrew University of Jerusalem, Institute of Chemistry, Center for Nanoscience and Nanotechnology, Jerusalem, 91904 (Israel).
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Aleman Garcia
authors.affiliation: The Hebrew University of Jerusalem, Institute of Chemistry, Center for Nanoscience and Nanotechnology, Jerusalem, 91904 (Israel). h.b.albada@gmail.com.
authors.forename: H Bauke
authors.initials: HB
authors.lastname: Albada
authors.affiliation: The Hebrew University of Jerusalem, Institute of Chemistry, Center for Nanoscience and Nanotechnology, Jerusalem, 91904 (Israel). willnea@vms.huji.ac.il.
authors.forename: Itamar
authors.initials: I
authors.lastname: Willner
mesh_terms: D021881:DNA, Catalytic / Q000378:metabolism*; D007700:Kinetics; D053758:Nanoparticles / Q000737:chemistry* / Q000378:metabolism*; D010316:Particle Size; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry* / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013499:Surface Properties
chemical_list: D021881:DNA, Catalytic; D012822:Silicon Dioxide
keywords: DNA nanotechnology; FRET; click chemistry; controlled release; fluorescence