title: Structure of the O-polysaccharide of the lipopolysaccharide of Pseudomonas chlororaphis subsp. aureofaciens UCM B-306.
abstract: Structure of the O-specific polysaccharide from Pseudomonas chlororaphis subsp. aureofaciens UCM B-306 was elucidated by sugar analysis along with 1D and 2D (1)H and (13)C NMR spectroscopy. The polysaccharide is built up of trisaccharide repeats containing D-rhamnose, 2,4-diacetamido-2,4,6-trideoxy-D-glucose (D-QuiNAc4NAc), and 2-acetamido-2-deoxy-D-galacturonic acid (D-GalNAcA), which is amidated in ∼40% repeats. It was suggested that the O-polysaccharide has a blockwise structure, which can be presented as follows: -[→3)-α-D-Rhap-(1→4)-α-D-GalpNAcA-(1→3)-α-D-QuipNAc4NAc-(1-]n→ and -[→3)-α-D-Rhap-(1→4)-α-D-GalpNAcAN-(1→3)-α-D-QuipNAc4NAc-(1-]m→, where GalNAcAN indicates 2-acetamido-2-deoxy-D-galacturonamide, n:m=∼3:2.
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, 119991 Moscow, Russia. Electronic address: zdorovenkoe@mail.ru.
authors.forename: Evelina L
authors.initials: EL
authors.lastname: Zdorovenko
authors.affiliation: D.K. Zabolotny Insitute of Microbiology and Virology, National Academy of Sciences, ul. Zabolotnogo 154, 03143 Kiev, Ukraine.
authors.forename: Liudmyla D
authors.initials: LD
authors.lastname: Varbanets
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, 119991 Moscow, Russia.
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.affiliation: D.K. Zabolotny Insitute of Microbiology and Virology, National Academy of Sciences, ul. Zabolotnogo 154, 03143 Kiev, Ukraine.
authors.forename: Elena A
authors.initials: EA
authors.lastname: Kiprianova
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, 119991 Moscow, Russia.
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
mesh_terms: D005947:Glucose / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D019081:O Antigens / Q000737:chemistry*; D011549:Pseudomonas / Q000737:chemistry*; D012210:Rhamnose / Q000737:chemistry*
chemical_list: C439356:2-acetamido-2-deoxy-galacturonic acid; D006603:Hexuronic Acids; D008070:Lipopolysaccharides; D019081:O Antigens; D005947:Glucose; D012210:Rhamnose
keywords: 2-Acetamido-2-deoxy-d-galacturonamide; Bacterial polysaccharide structure; Lipopolysaccharide; O-antigen; Pseudomonas chlororaphis