title: A hollow-fibre column system to effectively prepare washed platelets.
abstract: OBJECTIVE
  We developed a hollow-fibre column system specifically adapted to prepare washed platelet concentrates (WPCs). This study was performed to evaluate the efficacy of the hollow-fibre column system for preparing WPCs.
  
  
  METHODS
  First, the percentages of platelet (PLT) recovery and remaining plasma proteins were calculated by determining the PLT count, volume and plasma protein levels in both the prewash and postwash. Secondly, washed PLTs and unwashed control PLTs were stored for 5 days, and the changes during this 5-day storage of in vitro PLT characteristics were determined.
  
  
  RESULTS
  The hollow-fibre column system effectively removed >98% of plasma in platelet concentrates (PCs), and the PLT recovery was 97% on an average. The CD62P-expression level on washed PLTs immediately after washing was approximately twofold higher than that on prewashed PLTs as well as on PLTs washed via manual methods or cell washing devices. Until day 5 during storage, PLT aggregability, hypotonic shock response and swirling scores of washed PLTs were not significantly different from those of the control PCs.
  
  
  CONCLUSIONS
  Our novel hollow-fibre column system proved valuable in preparing washed PLTs with <2% of residual plasma proteins and high recovery of PLTs.
authors.affiliation: Japanese Red Cross Kinki Block Blood Centre, Ibaraki, Osaka, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Tanaka
authors.affiliation: Japanese Red Cross Kinki Block Blood Centre, Ibaraki, Osaka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Hayashi
authors.affiliation: Toray Industries, Inc., Otsu, Shiga, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Sugaya
authors.affiliation: Toray Industries, Inc., Otsu, Shiga, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Osabe
authors.affiliation: Toray Industries, Inc., Otsu, Shiga, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Ueno
authors.affiliation: Japanese Red Cross Kinki Block Blood Centre, Ibaraki, Osaka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Tani
authors.affiliation: Japanese Red Cross Kinki Block Blood Centre, Ibaraki, Osaka, Japan.
authors.forename: F
authors.initials: F
authors.lastname: Hirayama
mesh_terms: D001792:Blood Platelets / Q000166:cytology* / Q000378:metabolism; D001793:Blood Preservation / Q000295:instrumentation / Q000379:methods*; D005947:Glucose / Q000378:metabolism; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism; D013997:Time Factors
chemical_list: D019344:Lactic Acid; D005947:Glucose
keywords: hollow fibre; platelet; transfusion reactions; washed platelets