title: Identification of amino-acid residues in the V protein of peste des petits ruminants essential for interference and suppression of STAT-mediated interferon signaling.
abstract: Peste des petits ruminants virus (PPRV) causes a fatal disease in small ruminants. V protein of PPRV plays a pivotal role in interfering with host innate immunity by blocking IFNs signaling through interacting with STAT1 and STAT2. In the present study, the results demonstrated that PPRV V protein blocks IFN actions in a dose dependent manner and restrains the translocation of STAT1/2 proteins. We speculate that the translocation inhibition might be caused by the interfering of the downstream of STAT protein. Mutagenesis defines that Cys cluster and Trp motif of PPRV V protein are essential for STAT-mediated IFN signaling. These findings give a new sight for the further studies to understand the delicate mechanism of PPRV to escape the IFN signaling.
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Lanzhou 730030, PR China. Electronic address: maxushengtt@163.com.
authors.forename: Xusheng
authors.initials: X
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Lanzhou 730030, PR China.
authors.forename: Xing
authors.initials: X
authors.lastname: Yang
authors.affiliation: Institute of Pathogen Biology and Immunology, Hebei North University, Zhangjiakou 07500, PR China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Nian
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Lanzhou 730030, PR China.
authors.forename: Zhidong
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Lanzhou 730030, PR China.
authors.forename: Yongxi
authors.initials: Y
authors.lastname: Dou
authors.affiliation: Gansu Agricultural University, Lanzhou, PR China.
authors.forename: Xuehu
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Lanzhou 730030, PR China.
authors.forename: Xuenong
authors.initials: X
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Lanzhou 730030, PR China.
authors.forename: Junhong
authors.initials: J
authors.lastname: Su
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Lanzhou 730030, PR China.
authors.forename: Qiyun
authors.initials: Q
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Lanzhou 730030, PR China. Electronic address: caixp@vip.163.com.
authors.forename: Xuepeng
authors.initials: X
authors.lastname: Cai
mesh_terms: D000596:Amino Acids / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D004252:DNA Mutational Analysis; D006801:Humans; D057131:Immune Evasion; D007372:Interferons / Q000037:antagonists & inhibitors*; D020125:Mutation, Missense; D018107:Peste-des-petits-ruminants virus / Q000502:physiology*; D011485:Protein Binding; D060066:Protein Interaction Maps; D050794:STAT1 Transcription Factor / Q000378:metabolism*; D050795:STAT2 Transcription Factor / Q000378:metabolism*; D015398:Signal Transduction; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D050794:STAT1 Transcription Factor; C494079:STAT1 protein, human; D050795:STAT2 Transcription Factor; C494084:STAT2 protein, human; D014764:Viral Proteins; D007372:Interferons
keywords: IFN; PPRV; STAT1; STAT2; V protein