title: The Downregulation of MiR-182 Is Associated with the Growth and Invasion of Osteosarcoma Cells through the Regulation of TIAM1 Expression.
abstract: BACKGROUND
  Osteosarcoma is the most common primary bone malignancy in children and young adults. Increasing results suggest that discovery of microRNAs (miRNAs) might provide a novel therapeutical target for osteosarcoma.
  
  
  METHODS
  MiR-182 expression level in osteosarcoma cell lines and tissues were assayed by qRT-PCR. MiRNA mimics or inhibitor were transfected for up-regulation or down-regulation of miR-182 expression. Cell function was assayed by CCK8, migration assay and invasion assay. The target genes of miR-182 were predicated by bioinformatics algorithm (TargetScan Human).
  
  
  RESULTS
  MiR-182 was down-regulated in osteosarcoma tissues and cell lines. Overexpression of miR-182 inhibited tumor growth, migration and invasion. Subsequent investigation revealed that TIAM1 was a direct and functional target of miR-182 in osteosarcoma cells. Overexpression of miR-182 impaired TIAM1-induced inhibition of proliferation and invasion in osteosarcoma cells.
  
  
  CONCLUSIONS
  Down-expression of miR-182 in osteosarcoma promoted tumor growth, migration and invasion by targeting TIAM1. MiR-182 might act as a tumor suppressor gene whose down-regulation contributes to the progression and metastasis of osteosarcoma, providing a potential therapy target for osteosarcoma patients.
authors.affiliation: Department of Orthopedics, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China; Department of Orthopedics, The First Hospital of Kunming, Kunming, Yunnan, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Hu
authors.affiliation: Department of Orthopedics, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China.
authors.forename: Guohua
authors.initials: G
authors.lastname: Lv
authors.affiliation: Department of Orthopedics, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China; Department of Orthopedics, The Affiliated Hospital of Hunan University of Medicine and The Third People's Hospital of Huaihua, Huaihua, Hunan, China.
authors.forename: Shuguang
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Department of Orthopedics, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China; Department of Orthopedics, The People's Hospital of Qingyuan, Qingyuan, Guangdong, China.
authors.forename: Yucheng
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Orthopedics, The First Hospital of Kunming, Kunming, Yunnan, China.
authors.forename: Bangxu
authors.initials: B
authors.lastname: Nie
authors.affiliation: Department of Orthopedics, The First Hospital of Kunming, Kunming, Yunnan, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Duan
authors.affiliation: Department of Orthopedics, The First Hospital of Kunming, Kunming, Yunnan, China.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, The First Hospital of Kunming, Kunming, Yunnan, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Yuan
mesh_terms: D000293:Adolescent; D001859:Bone Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002465:Cell Movement; D049109:Cell Proliferation; D002648:Child; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D018716:Molecular Mimicry; D009361:Neoplasm Invasiveness; D009843:Oligoribonucleotides / Q000235:genetics / Q000378:metabolism; D012516:Osteosarcoma / Q000235:genetics* / Q000378:metabolism; D061251:Primary Cell Culture; D015398:Signal Transduction; D000075684:T-Lymphoma Invasion and Metastasis-inducing Protein 1; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D020662:Guanine Nucleotide Exchange Factors; D035683:MicroRNAs; C538984:Mirn182 microRNA, human; D009843:Oligoribonucleotides; D000075684:T-Lymphoma Invasion and Metastasis-inducing Protein 1; C087334:TIAM1 protein, human