title: Purification, Partial Characterizations, and N-Terminal Amino Acid Sequence of a Procoagulant Protein FV-2 from Daboia Russelli Siamensis (Myanmar) Venom.
abstract: In this study, we purified and characterized the procoagulant protein FV-2 from Daboia russelli siamensis (Myanmar) venom using ion-exchange chromatography on CM-Sephadex C-50 and gel filtration on SuperdexTMG-75 column. The activation of factor X and prothrombin was determined, respectively, by specific chromogenic substrates. The fibrinogen-clotting activity, thermal stability, and pH stability were also determined. The N-treminal sequence was determined by the National Center of Biomedical Analysis of China. In the end, FV-2 was achieved with a molecular weight of 13,608.0 Da. It could activate factor X, but did not affect prothrombin or fibrinogen. The suitable pH was 6.5-7.5, and the suitable temperature ranged from 25 to 60°C. The N-terminal sequence was Asn-Phe-Phe-Gln-Phe-Ala-Glu-Met-Ile-Val-Lys-Met-Thr-Gly-Lys. Taken together, our studies suggest that FV-2 is a factor X-activating enzyme, which can activate factor X to factor Xa, but it has no effect on prothrombin and fibrinogen.
authors.affiliation: Department of Pharmacology, Medical College, Jinan University, Guangzhou, 510632, People's Republic of China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, Guangzhou, 510632, People's Republic of China.|Department of Developmental and Regenerative Biology, Jinan University, Guangzhou, 510632, People's Republic of China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Department of Pharmacology, Medical College, Jinan University, Guangzhou, 510632, People's Republic of China.
authors.forename: Shuhao
authors.initials: S
authors.lastname: Shen
authors.affiliation: Department of Physiology, Medical College, Jinan University, Guangzhou, 510632, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Pharmacology, Medical College, Jinan University, Guangzhou, 510632, People's Republic of China.
authors.forename: Nanlin
authors.initials: N
authors.lastname: Xiang
authors.affiliation: Department of Pharmacology, Medical College, Jinan University, Guangzhou, 510632, People's Republic of China.
authors.forename: Yunjian
authors.initials: Y
authors.lastname: Liao
authors.affiliation: Department of Pharmacology, Medical College, Jinan University, Guangzhou, 510632, People's Republic of China.
authors.forename: Xi
authors.initials: X
authors.lastname: Lin
authors.affiliation: Department of Pharmacology, Medical College, Jinan University, Guangzhou, 510632, People's Republic of China.
authors.forename: Lixin
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Pharmacology and Toxicology, Guangdong Institute for Food and Drug Control, Guangdong Province, Guangzhou, 510180, People's Republic of China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Chen
keywords: Daboia russelli siamensis; N-terminal sequence; Snake venom; factor X activator; procoagulant activity