title: Reactivation of Giardia lamblia cysts after exposure to low-pressure UV irradiation.
abstract: In this study, we determined the repair capabilities of Giardia lamblia cysts when they were exposed to low-pressure (LP) UV and then 4 different repair conditions. A UV collimated beam apparatus was used to expose shallow suspensions of G. lamblia cysts in buffered reagent water (PBS, pH 7.2) to various doses of LP UV irradiation. After UV irradiation, samples were exposed to 4 repair conditions (light and dark repair conditions with 2 temperatures (25 °C and 37 °C) for each condition). The inactivation of G. lamblia cysts by LP UV was very extensive (∼ 5 log10) even with a low dose of LP UV (1 mJ/cm(2)). More importantly, there was significant restoration of infectivity in G. lamblia cysts when they were exposed to a low dose of LP UV and then to all the repair conditions tested. Overall, the results of this study indicate that G. lamblia cysts do have the ability to repair their UV-damaged DNA when they are exposed to low doses of LP UV irradiation. This is the first study to report the presence of repair in UV-irradiated G. lamblia cysts.
authors.affiliation: a Department of Environmental Engineering, Ajou University, 206 World cup-ro, Yeongtong-gu, Suwon-si, Gyeonggi-do, South Korea, 443-749.
authors.forename: Gwy-Am
authors.initials: GA
authors.lastname: Shin
authors.affiliation: b Civil, Environmental, and Architectural Engineering, University of Colorado at Boulder, 428 UCB, Boulder, CO 80309, USA.
authors.forename: Karl G
authors.initials: KG
authors.lastname: Linden
mesh_terms: D004249:DNA Damage / Q000528:radiation effects; D016829:Giardia lamblia / Q000235:genetics / Q000254:growth & development* / Q000502:physiology / Q000528:radiation effects*; D011312:Pressure; D013696:Temperature; D014466:Ultraviolet Rays
keywords: DNA repair; Giardia lamblia cysts; UV à basse pression; gerbil infectivity assay; kystes de Giardia lamblia; low pressure UV; réparation d’ADN; épreuve d’infectiosité dans des gerbilles