title: Comparing seven mitogens with PHA-M for improved lymphocyte stimulation in dicentric chromosome analysis for biodosimetry.
abstract: Dicentric chromosome analysis (DCA) is the gold standard for individual radiation dose estimation. Two limiting factors of DCA are the time-consuming lymphocyte stimulation and proliferation using the lectin PHA-M and the upper dose limit of individual dose assessment of ∼4 Gy. By measuring the mitotic index (MI), the authors investigated systematically whether the stimulation of lymphocytes can be improved after administration of alternative (and combined) mitogens. The authors compared the lymphocyte stimulation effectiveness of the traditionally used PHA-M (from Phaseolus vulgaris) with seven cited mitogens by determination of MIs: five lectins namely CNA (concanavalin A), PW (pokeweed), LMA (Maackia amurensis), LTV (T. vulgaris), PHA-L (P. vulgaris) as well as LPS (lipopolysaccharide, Escherichia coli) and SLO (streptolysine O, Streptococcus pyogenes) were applied. The conventional protocol using PHA-M for lymphocyte stimulation proved to be superior over lower/higher PHA-M concentrations as well as seven other mitogens administered either alone or combined with SLO or LPS.
authors.affiliation: Bundeswehr Institute of Radiobiology Affiliated to the University Ulm, Neuherbergstr. 11, Munich 80937, Germany christinabeinke@bundeswehr.org.
authors.forename: C
authors.initials: C
authors.lastname: Beinke
authors.affiliation: Bundeswehr Institute of Radiobiology Affiliated to the University Ulm, Neuherbergstr. 11, Munich 80937, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Port
authors.affiliation: Bundeswehr Institute of Radiobiology Affiliated to the University Ulm, Neuherbergstr. 11, Munich 80937, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Lamkowski
authors.affiliation: Bundeswehr Institute of Radiobiology Affiliated to the University Ulm, Neuherbergstr. 11, Munich 80937, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Abend
mesh_terms: D002869:Chromosome Aberrations / Q000528:radiation effects*; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects* / Q000528:radiation effects; D008214:Lymphocytes / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects; D008934:Mitogens / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology*; D011829:Radiation Dosage; D011834:Radiation Monitoring / Q000379:methods*
chemical_list: D008934:Mitogens; D010835:Phytohemagglutinins; C047876:phytohemagglutinin-M