title: Clinical Significance of miR-1826 as a Novel Prognostic Biomarker in Colorectal Cancer.
abstract: Several reports indicated that aberrant miR-1826 was involved in the initiation and progression of malignancies. However, the clinical significance of miR-1826 in human colorectal cancer (CRC) has not been addressed. The aim of this study was to evaluate the expression and clinical significance of miR-1826 in CRC. We detected miR-1826 expressions by quantitative real.time PCR (qRT-PCR) in 72 CRC tissues, adjacent non.tumor tissues (NATs) and five CRC cell lines, and found that miR-1826 expression in CRC tissues was higher than that in NATs (p<0.05). High miR-1826 expression was significantly associated with some clinicopathologic features such as regional lymph node metastasis (p=0.018), advanced TNM clinical stage (p=0.004), which led to a poor overall survival rate in CRC patients (p=0.032). Our further studies in vitro also demonstrated that miR-1826 inhibitor could effectively suppress the cell proliferation, promote apoptosis and induce G0/G1 cell cycle arrest in CRC cells (p<0.05). Meanwhile, the abilities of cell invasion and migration were effectively suppressed by miR-1826 inhibitor (p<0.05). These findings strongly suggested that miR-1826 played a critical role in the initiation and progression of CRC. Up-regulation of miR-1826 might serve as a novel prognostic marker of CRC and could be a potential target for CRC therapies.
authors.forename: You
authors.initials: Y
authors.lastname: Hu
authors.forename: Bin
authors.initials: B
authors.lastname: Yi
authors.forename: Songbing
authors.initials: S
authors.lastname: He
authors.forename: Ling
authors.initials: L
authors.lastname: Gao
authors.forename: Pengfei
authors.initials: P
authors.lastname: Liu
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Yu
authors.forename: Daiwei
authors.initials: D
authors.lastname: Wan
authors.forename: Jin
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of General Surgery, First Affiliated Hospital of Soochow University, Soochow, 215006, China. zhuxinguo40@163.com.
authors.forename: Xinguo
authors.initials: X
authors.lastname: Zhu
authors.affiliation: . strexboy@163.com.
authors.forename: Qiaoming
authors.initials: Q
authors.lastname: Zhi
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics; D002453:Cell Cycle; D045744:Cell Line, Tumor; D002465:Cell Movement; D003106:Colon / Q000378:metabolism / Q000473:pathology*; D015179:Colorectal Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000032:analysis / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D011379:Prognosis; D012007:Rectum / Q000378:metabolism / Q000473:pathology*; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C570042:MIRN1826 microRNA, human; D035683:MicroRNAs