title: Determination of veterinary antibiotics in bovine urine by liquid chromatography-tandem mass spectrometry.
abstract: A follow-up of antibiotics (tetracyclines, fluoroquinolones, cephalosporins, penicillins and amphenicols) in the bovine urine is important for two reasons: to understand if they are still present in organism, and whether their occurrence in urine might be considered as an environmental risk. A validated HPLC-MS/MS method (Decision 2002/657/EC) for antibiotics determination in bovine urine was developed. CCα and CCβ were in the range of 0.58-0.83 and 0.55-1.1 ng mL(-1), respectively. Recoveries were 92-108%, with inter-day repeatability below 12%. Analysis of bovine urine revealed frequent presence of tetracyclines, which was related with animal's age. The cause, most presumably, might be found in different therapeutic protocols applied for veal calves and young bulls enrolled in this study. Most abundant was oxytetracycline with highest level in veal calves (1718 ng mL(-1)) vs. young bulls (2.8 ng mL(-1)). Our results indicate the necessity of antibiotics monitoring in bovine urine before animals undergo further processing in the food industry.
authors.affiliation: Department of Veterinary Science and Public Health, University of Milan, Via Celoria 10, 20133 Milan, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Chiesa
authors.affiliation: Department of Veterinary Science and Public Health, University of Milan, Via Celoria 10, 20133 Milan, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Nobile
authors.affiliation: Department of Health, Animal Science and Food Safety, University of Milan, Via Celoria 10, 20133 Milan, Italy. Electronic address: francesco.arioli@unimi.it.
authors.forename: Francesco
authors.initials: F
authors.lastname: Arioli
authors.affiliation: Department of Health Sciences V. le Europa, Campus S. Venuta, Germaneto, 88100 Catanzaro, Italy.
authors.forename: Domenico
authors.initials: D
authors.lastname: Britti
authors.affiliation: Department of Chemistry, Faculty of Medicine, University of Nis, Bulevar Dr. Zorana Djindjica 81, 18000 Nis, Serbia.
authors.forename: Natasa
authors.initials: N
authors.lastname: Trutic
authors.affiliation: Department of Veterinary Science and Public Health, University of Milan, Via Celoria 10, 20133 Milan, Italy; Department of Chemistry, Faculty of Medicine, University of Nis, Bulevar Dr. Zorana Djindjica 81, 18000 Nis, Serbia.
authors.forename: Radmila
authors.initials: R
authors.lastname: Pavlovic
authors.affiliation: Department of Veterinary Science and Public Health, University of Milan, Via Celoria 10, 20133 Milan, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Panseri
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000652:urine*; D002417:Cattle; D002511:Cephalosporins / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D024841:Fluoroquinolones / Q000652:urine; D008297:Male; D010406:Penicillins / Q000652:urine; D053719:Tandem Mass Spectrometry; D013754:Tetracyclines / Q000652:urine; D019155:Veterinary Drugs / Q000652:urine*
chemical_list: D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D024841:Fluoroquinolones; D010406:Penicillins; D013754:Tetracyclines; D019155:Veterinary Drugs
keywords: Amphenicols; Bovine urine; Cephalosporins; Fluoroquinolones; LC–MS/MS; Penicillins; Tetracyclines