title: Evaluation of a metalloporphyrin (THPPMnCl) for necrosis-affinity in rat models of necrosis.
abstract: The combination of an (13I)I-labeled necrosis-targeting agent (NTA) with a vascular disrupting agent is a novel and potentially powerful technique for tumor necrosis treatment (TNT). The purpose of this study was to evaluate a NTA candidate, THPPMnCl, using (131)I isotope for tracing its biodistribution and necrosis affinity. (131)I-THPPMnCl was intravenously injected in rat models with liver, muscle, and tumor necrosis and myocardial infarction (MI), followed by investigations with macroscopic autoradiography, triphenyltetrazolium chloride (TTC) histochemical staining, fluorescence microscopy and H&E stained histology for up to 9 days. (131)I-THPPMnCl displayed a long-term affinity for all types of necrosis and accumulation in the mononuclear phagocytic system especially in the liver. Autoradiograms and TTC staining showed a good targetability of (131)I-THPPMnCl for MI. These findings indicate the potential of THPPMnCl for non-invasive imaging assessment of necrosis, such as in MI. However, (13I)I-THPPMnCl is unlikely suitable for TNT due to its long-term retention in normal tissues.
authors.affiliation: a Laboratory of Translational Medicine, Jiangsu Province Academy of Traditional Chinese Medicine , Nanjing , Jiangsu Province , P.R. China and.
authors.forename: Yue
authors.initials: Y
authors.lastname: Li
authors.affiliation: a Laboratory of Translational Medicine, Jiangsu Province Academy of Traditional Chinese Medicine , Nanjing , Jiangsu Province , P.R. China and.
authors.forename: Xuejiao
authors.initials: X
authors.lastname: Liu
authors.affiliation: a Laboratory of Translational Medicine, Jiangsu Province Academy of Traditional Chinese Medicine , Nanjing , Jiangsu Province , P.R. China and.
authors.forename: Dongjian
authors.initials: D
authors.lastname: Zhang
authors.affiliation: a Laboratory of Translational Medicine, Jiangsu Province Academy of Traditional Chinese Medicine , Nanjing , Jiangsu Province , P.R. China and.
authors.forename: Bin
authors.initials: B
authors.lastname: Lou
authors.affiliation: a Laboratory of Translational Medicine, Jiangsu Province Academy of Traditional Chinese Medicine , Nanjing , Jiangsu Province , P.R. China and.
authors.forename: Fei
authors.initials: F
authors.lastname: Peng
authors.affiliation: a Laboratory of Translational Medicine, Jiangsu Province Academy of Traditional Chinese Medicine , Nanjing , Jiangsu Province , P.R. China and.
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Wang
authors.affiliation: a Laboratory of Translational Medicine, Jiangsu Province Academy of Traditional Chinese Medicine , Nanjing , Jiangsu Province , P.R. China and.
authors.forename: Xin
authors.initials: X
authors.lastname: Shan
authors.affiliation: a Laboratory of Translational Medicine, Jiangsu Province Academy of Traditional Chinese Medicine , Nanjing , Jiangsu Province , P.R. China and.
authors.forename: Cuihua
authors.initials: C
authors.lastname: Jiang
authors.affiliation: a Laboratory of Translational Medicine, Jiangsu Province Academy of Traditional Chinese Medicine , Nanjing , Jiangsu Province , P.R. China and.
authors.forename: Meng
authors.initials: M
authors.lastname: Gao
authors.affiliation: b Radiation Medical Institute, Shandong Academy of Medical Sciences , Jinan , Shandong Province , P.R. China , and.
authors.forename: Ziping
authors.initials: Z
authors.lastname: Sun
authors.affiliation: a Laboratory of Translational Medicine, Jiangsu Province Academy of Traditional Chinese Medicine , Nanjing , Jiangsu Province , P.R. China and.|c Department of Radiology , KU Leuven , Leuven , Belgium.
authors.forename: Yicheng
authors.initials: Y
authors.lastname: Ni
authors.affiliation: a Laboratory of Translational Medicine, Jiangsu Province Academy of Traditional Chinese Medicine , Nanjing , Jiangsu Province , P.R. China and.
authors.forename: Dejian
authors.initials: D
authors.lastname: Huang
authors.affiliation: a Laboratory of Translational Medicine, Jiangsu Province Academy of Traditional Chinese Medicine , Nanjing , Jiangsu Province , P.R. China and.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001345:Autoradiography; D004195:Disease Models, Animal; D008665:Metalloporphyrins / Q000494:pharmacology* / Q000627:therapeutic use; D008856:Microscopy, Fluorescence; D009336:Necrosis / Q000188:drug therapy*; D051381:Rats; D013778:Tetrazolium Salts; D014018:Tissue Distribution
chemical_list: D008665:Metalloporphyrins; D013778:Tetrazolium Salts; C009591:triphenyltetrazolium
keywords: Autoradiography; THPPMnCl; biodistribution; microscopic fluorescence imaging; necrosis-targeting; pharmacokinetics