title: A study of RUNX3, E-cadherin and β-catenin in CagA-positive Helicobacter pylori associated chronic gastritis in Saudi patients.
abstract: OBJECTIVE
  H. pylori is the most important risk factor for gastric carcinoma. CagA-positive H. pylori is associated with an increased risk for gastric cancer compared with negative strains. RUNX3 is a tumor suppressor gene, which is related to the genesis of gastric cancer. β-catenin is integrated with E-cadherin in the cell membrane, and aberrant expression of the complex was reported in gastric carcinoma. Aim of this paper is to determine of the relation between RUNX3, E-cadherin and β-catenin in chronic gastritis associated with cagA-positive H. pylori infection.
  
  
  METHODS
  Retrospective study was done on formalin fixed paraffin embedded gastric biopsies blocks of 90 patients diagnosed as H. pylori associated chronic gastritis. H. pylori was detected using modified Giemsa stain. Nested PCR was used for detection of cagA, reverse transcription-PCR for detection of RUNX3 and immunohistochemistry for detection of E-cadherin and β-catenin.
  
  
  RESULTS
  Fifty percent of cases were found to be cagA positive. CagA was significantly associated with the intensity of mononuclear inflammation, the intensity of neutrophilic inflammation, the degree of mucosal atrophy and loss of RUNX3 but not with the density of H. pylori, intestinal metaplasia, E-cadherin or β-catenin. There was significant relation between loss of RUNX3 and increasing density of H. pylori, intensity of neutrophilic inflammation, mucosal atrophy and intestinal metaplasia. RUNX3 was found to be significantly correlated with E-cadherin but not with β-catenin. E-cadherin showed decreased expression in 36.7% of biopsies while, β-catenin was decreased in 33% of biopsies.
  
  
  CONCLUSIONS
  Loss of RUNX3, E-cadherin and β-catenin was considered early events in the cascade of gastric carcinoma development. Loss of RUNX3 but neither E-cadherin nor β-catenin was related to cagA positive H. pylori strains.
authors.affiliation: Medical Laboratories Technology Department, Faculty of Applied Medical Sciences, Taibah University, Saudi Arabia; Pathology Department, Faculty of Medicine, Suez Canal University, Egypt. hrwagih@yahoo.com.
authors.forename: H M
authors.initials: HM
authors.lastname: Wagih
authors.forename: S M
authors.initials: SM
authors.lastname: El-Ageery
authors.forename: A A
authors.initials: AA
authors.lastname: Alghaithy
mesh_terms: D000293:Adolescent; D000328:Adult; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D015820:Cadherins / Q000172:deficiency*; D002908:Chronic Disease; D050677:Core Binding Factor Alpha 3 Subunit / Q000172:deficiency*; D005260:Female; D005753:Gastric Mucosa / Q000382:microbiology / Q000473:pathology; D005756:Gastritis / Q000175:diagnosis / Q000453:epidemiology / Q000378:metabolism*; D016481:Helicobacter Infections / Q000175:diagnosis / Q000453:epidemiology / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D012529:Saudi Arabia / Q000453:epidemiology; D013274:Stomach Neoplasms / Q000175:diagnosis / Q000453:epidemiology / Q000378:metabolism; D055815:Young Adult; D051176:beta Catenin / Q000172:deficiency*
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C495270:CTNNB1 protein, human; D015820:Cadherins; D050677:Core Binding Factor Alpha 3 Subunit; C493729:Runx3 protein, human; D051176:beta Catenin; C080866:cagA protein, Helicobacter pylori