title: CTLs regulate tumor growth via cytostatic effects rather than cytotoxicity: a few T cells can influence the growth of many times more tumor cells.
abstract: Cytotoxic T lymphocytes (CTLs) play a central role in antitumor immunity. We utilized B16 melanoma cells expressing the fluorescent ubiquitination-based cell cycle indicator B16-fucci implanted in host mice and adoptively transferred with pmel-1-TCR transgenic T cells to demonstrate that tumor growth reduction is largely dependent on interferon γ-mediated cell cycle arrest rather than the cytotoxic killing of tumor cells by CTLs.
authors.affiliation: Department of Immunotherapeutic; The University of Tokyo Hospital ; Tokyo, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kakimi
authors.affiliation: Department of Immunotherapeutic; The University of Tokyo Hospital ; Tokyo, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Matsushita
authors.affiliation: Department of Immunotherapeutic; The University of Tokyo Hospital ; Tokyo, Japan ; Medinet Co Ltd.; Yokohama , Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Hosoi
authors.affiliation: Department of Immunotherapeutic; The University of Tokyo Hospital ; Tokyo, Japan ; Medinet Co Ltd.; Yokohama , Japan.
authors.forename: Manami
authors.initials: M
authors.lastname: Miyai
authors.affiliation: Department of Human Genome Research; Kazusa DNA Research Institute ; Chiba, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Ohara
keywords: CTL; IFN-γ; cell cycle arrest; fucci system; tumor inhibition