title: Rapid Detection of Bacillus anthracis in Complex Food Matrices Using Phage-Mediated Bioluminescence.
abstract: Bacillus anthracis, the causative agent of anthrax, is considered a high-priority agent that may be used in a food-related terrorist attack because it can be contracted by ingestion and it also forms spores with heat and chemical resistance. Thus, novel surveillance methodologies to detect B. anthracis on adulterated foods are important for bioterrorism preparedness. We describe the development of a phage-based bioluminescence assay for the detection of B. anthracis on deliberately contaminated foods. We previously engineered the B. anthracis phage Wβ with genes encoding bacterial luciferase (luxA and luxB) to create a "light-tagged" reporter (Wβ::luxAB) that is able to rapidly detect B. anthracis by transducing a bioluminescent signal response. Here, we investigate the ability of Wβ::luxAB to detect B. anthracis Sterne, an attenuated select agent strain, in inoculated food (ground beef) and milk (2%, baby formula, and half and half) matrices after incubation with spores for 72 h at 4°C as per AOAC testing guidelines. The majority of B. anthracis bacilli remained in spore form, and thus were potentially infectious, within each of the liquid matrices for 14 days. Detection limits were 80 CFU/ml after 7 h of enrichment; sensitivity of detection increased to 8 CFU/ml when enrichment was extended to 16 h. The limit of detection in ground beef was 3.2 × 10(3) CFU/g after 7 h of enrichment, improving to 3.2 × 10(2) CFU/g after 16 h. Because the time to result is rapid and minimal processing is required, and because gastrointestinal anthrax can be fatal, the reporter technology displays promise for the protection of our food supply following a deliberate release of this priority pathogen.
authors.affiliation: Guild BioSciences, 1313B Ashley River Road, Charleston, South Carolina 29407, USA.
authors.forename: Natasha J
authors.initials: NJ
authors.lastname: Sharp
authors.affiliation: Guild BioSciences, 1313B Ashley River Road, Charleston, South Carolina 29407, USA.
authors.forename: Joshua P
authors.initials: JP
authors.lastname: Vandamm
authors.affiliation: Molecular Biosciences, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas 78712, USA.
authors.forename: Ian J
authors.initials: IJ
authors.lastname: Molineux
authors.affiliation: Guild BioSciences, 1313B Ashley River Road, Charleston, South Carolina 29407, USA. dschofield@guildbiosciences.com.
authors.forename: David A
authors.initials: DA
authors.lastname: Schofield
mesh_terms: D000818:Animals; D001408:Bacillus anthracis / Q000302:isolation & purification*; D001435:Bacteriophages; D002417:Cattle; D005504:Food Analysis; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D005517:Foodborne Diseases / Q000517:prevention & control; D049453:Luciferases, Bacterial / Q000235:genetics / Q000378:metabolism; D008163:Luminescent Measurements; D008460:Meat / Q000382:microbiology; D008892:Milk / Q000382:microbiology
chemical_list: D049453:Luciferases, Bacterial