title: Domestic wastewater treatment in a novel sequencing batch biofilm filter.
abstract: Biological treatment of domestic sewage low C/N ratio was accomplished in a pilot-scale sequencing batch biofilm filter (SBBF). The novel hybrid bioreactor consisted of bio-band in the upper and anthracite filter media in the bottom, which combined a sequencing batch biofilm reactor (SBBR) with a biological filter. The average removal efficiency values of chemical oxygen demand (COD), ammonium nitrogen (NH4 (+)-N), total nitrogen (TN), and total phosphorus (TP) were 89.4, 83.3, 62.9, and 48.7%, respectively. A 454-pyrosequencing technology was employed to investigate the microbial communities of the influent (J1) and the biofilm (J2) on the bio-band on day 40. Pyrosequencing analysis of the 16S rRNA gene revealed the community of the biofilm consisted of Gammaproteobacteria (48.6%), Planctomycetacia (18.0%), Alphaproteobacteria (13.7%), Clostridia (9.6%), Desulfonatronum (18.5%), Actinobacteria (1.9%), and Bacilli (1.7%), accounting for 93.6% of total operational taxonomic units at genera level. Acinetobacter tjernbergiae and Acinetobacter lwoffii were the most abundant species, suggesting that denitrifying phosphorus removal was achieved in the SBBF.
authors.affiliation: School of Civil Engineering, Wuhan University, Wuhan, 430072, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Ji
authors.forename: Li
authors.initials: L
authors.lastname: Wei
authors.forename: Dan
authors.initials: D
authors.lastname: Chen
authors.forename: Hongyu
authors.initials: H
authors.lastname: Wang
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Li
authors.forename: Kai
authors.initials: K
authors.lastname: Yang
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics; D044822:Biodiversity; D018441:Biofilms / Q000254:growth & development*; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005374:Filtration / Q000379:methods*; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000032:analysis; D010758:Phosphorus / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D062065:Wastewater / Q000737:chemistry* / Q000382:microbiology; D018508:Water Purification / Q000379:methods*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D009584:Nitrogen; D010758:Phosphorus; D012336:RNA, Ribosomal, 16S; D062065:Wastewater