title: Distinct effects of ubiquitin overexpression on NMJ structure and motor performance in mice expressing catalytically inactive USP14.
abstract: Ubiquitin-specific protease 14 (USP14) is a major deubiquitinating enzyme and a key determinant of neuromuscular junction (NMJ) structure and function. We have previously reported dramatic ubiquitin depletion in the nervous systems of the USP14-deficient ataxia (ax (J) ) mice and demonstrated that transgenic ubiquitin overexpression partially rescues the ax (J) neuromuscular phenotype. However, later work has shown that ubiquitin overexpression does not correct the ax (J) deficits in hippocampal short term plasticity, and that transgenic expression of a catalytically inactive form of USP14 in the nervous system mimics the neuromuscular phenotype observed in the ax (J) mice, but causes a only a modest reduction of free ubiquitin. Instead, increased ubiquitin conjugates and aberrant activation of pJNK are observed in the nervous systems of the USP14 catalytic mutant mice. In this report, we demonstrate that restoring free ubiquitin levels in the USP14 catalytic mutant mice improved NMJ structure and reduced pJNK accumulation in motor neuron terminals, but had a negative impact on measures of NMJ function, such as motor performance and muscle development. Transgenic expression of ubiquitin had a dose-dependent effect on NMJ function in wild type mice: moderate levels of overexpression improved NMJ function while more robust ubiquitin overexpression reduced muscle development and motor coordination. Combined, these results suggest that maintenance of free ubiquitin levels by USP14 contributes to NMJ structure, but that USP14 regulates NMJ function through a separate pathway.
authors.affiliation: Evelyn F. McKnight Brain Institute, Department of Neurobiology and Civitan International Research Center, University of Alabama at Birmingham Birmingham, AL, USA.
authors.forename: Jada H
authors.initials: JH
authors.lastname: Vaden
authors.affiliation: Evelyn F. McKnight Brain Institute, Department of Neurobiology and Civitan International Research Center, University of Alabama at Birmingham Birmingham, AL, USA.
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: Watson
authors.affiliation: Evelyn F. McKnight Brain Institute, Department of Neurobiology and Civitan International Research Center, University of Alabama at Birmingham Birmingham, AL, USA.
authors.forename: Alan D
authors.initials: AD
authors.lastname: Howard
authors.affiliation: Department of Structural Biology, St. Jude Children's Research Hospital Memphis, TN, USA.
authors.forename: Ping-Chung
authors.initials: PC
authors.lastname: Chen
authors.affiliation: Evelyn F. McKnight Brain Institute, Department of Neurobiology and Civitan International Research Center, University of Alabama at Birmingham Birmingham, AL, USA.
authors.forename: Julie A
authors.initials: JA
authors.lastname: Wilson
authors.affiliation: Evelyn F. McKnight Brain Institute, Department of Neurobiology and Civitan International Research Center, University of Alabama at Birmingham Birmingham, AL, USA.
authors.forename: Scott M
authors.initials: SM
authors.lastname: Wilson
keywords: USP14; motor neuron and deubiquitinating enzyme; neuromuscular junction; pJNK; proteasomes; ubiquitin