title: PKCη Regulates the TGFβ3-induced Chondevrepogenic Differentiation of Human Mesenchymal Stem Cell.
abstract: Transforming growth factor (TGF) family is well known to induce the chondevrepogenic differentiation of mesenchymal stem cells (MSC). However, the precise signal transduction pathways and underlying factors are not well known. Thus the present study aims to evaluate the possible role of C2 domain in the chondevrepogenic differentiation of human mesenchymal stem cells. To this end, 145 C2 domains in the adenovirus were individually transfected to hMSC, and morphological changes were examined. Among 145 C2 domains, C2 domain of protein kinase C eta (PKCη) was selected as a possible chondevrepogenic differentiation factor for hMSC. To confirm this possibility, we treated TGFβ3, a well known chondevrepogenic differentiation factor of hMSC, and examined the increased-expression of glycosaminoglycan (GAG), collagen type II (COL II) as well as PKCη using PT-PCR, immunocytochemistry and Western blot analysis. To further evaluation of C2 domain of PKCη, we examined morphological changes, expressions of GAG and COL II after transfection of PKCη -C2 domain in hMSC. Overexpression of PKCη-C2 domain induced morphological change and increased GAG and COL II expressions. The present results demonstrate that PKCη involves in the TGF-β3-induced chondevrepogenic differentiation of hMSC, and C2 domain of PKCη has important role in this process.
authors.affiliation: Department of Anatomy & Neurobiology, Institute of Health Sciences, School of Medicine, Gyeongsang National University, Jinju 660-290, Republic of Korea.
authors.forename: Bo Mi
authors.initials: BM
authors.lastname: Ku
authors.affiliation: Department of Anatomy & Neurobiology, Institute of Health Sciences, School of Medicine, Gyeongsang National University, Jinju 660-290, Republic of Korea.
authors.forename: Young Phil
authors.initials: YP
authors.lastname: Yune
authors.affiliation: Department of Rehabilitation Medicine, School of Medicine, Gyeongsang National University, Jinju 660-290, Republic of Korea.
authors.forename: Eun Shin
authors.initials: ES
authors.lastname: Lee
authors.affiliation: Clinical Research Institute, Gyeongsang National University Hospital, Jinju 660-702, Republic of Korea.
authors.forename: Young-Sool
authors.initials: YS
authors.lastname: Hah
authors.affiliation: Dept. of Physiology, School of Medicine, Gyeongsang National University, Jinju 660-751, Republic of Korea.
authors.forename: Jae Yong
authors.initials: JY
authors.lastname: Park
authors.affiliation: Department of Anatomy & Neurobiology, Institute of Health Sciences, School of Medicine, Gyeongsang National University, Jinju 660-290, Republic of Korea.
authors.forename: Joo Yeon
authors.initials: JY
authors.lastname: Jeong
authors.affiliation: Department of Anatomy & Neurobiology, Institute of Health Sciences, School of Medicine, Gyeongsang National University, Jinju 660-290, Republic of Korea.
authors.forename: Dong Hoon
authors.initials: DH
authors.lastname: Lee
authors.affiliation: Department of Anatomy & Neurobiology, Institute of Health Sciences, School of Medicine, Gyeongsang National University, Jinju 660-290, Republic of Korea.
authors.forename: Gyeong Jae
authors.initials: GJ
authors.lastname: Cho
authors.affiliation: Department of Anatomy & Neurobiology, Institute of Health Sciences, School of Medicine, Gyeongsang National University, Jinju 660-290, Republic of Korea.
authors.forename: Wan Sung
authors.initials: WS
authors.lastname: Choi
authors.affiliation: Department of Anatomy & Neurobiology, Institute of Health Sciences, School of Medicine, Gyeongsang National University, Jinju 660-290, Republic of Korea.
authors.forename: Sang Soo
authors.initials: SS
authors.lastname: Kang
keywords: C2-domain.; Chondevrepogenesis; Human mesenchymal stem cell; PKCη; TGF-β3