title: Effect of polymorphisms of vascular endothelial growth factor on prognosis in osteosarcoma patients.
abstract: We investigated the association between vascular endothelial growth factor (VEGF) gene +1612G/A, -634C/G, and +936G/C and the clinical outcome of osteosarcoma. Genomic DNA was isolated from blood samples, and 3 VEGF gene polymorphisms (+1612G/A, -634C/G, and +936G/C) were analyzed using polymerase chain reaction-restriction fragment length polymorphism. Of the 194 patients, 82 patients (42.27%) showed a good response to chemotherapy, while 73 (37.63%) died during the follow-up period. When comparing good and poor responders, we observed no significant association between the VEGF +1612G/A, -634G/C, and +936T/C polymorphisms and clinical outcome of osteosarcoma patients. According to Cox regression analysis, the VEGF +1612A/G, -634G/C, and +936T/C polymorphisms did not statistically significantly increase the risk of overall survival of patients with osteosarcoma. This study showed that VEGF +1612A/G, -634G/C, and +936T/C polymorphisms were not related to the response to chemotherapy and clinical outcome of osteosarcoma patients.
authors.affiliation: Department of Joint Surgery, Zhejiang Provincial Corps Hospital, Chinese People's Armed Police Forces, JiaXing, China lixiaodong6676@163.com.
authors.forename: X D
authors.initials: XD
authors.lastname: Li
authors.affiliation: Department of Ophthalmology, Zhejiang Provincial Corps Hospital, Chinese People's Armed Police Forces, JiaXing, China.
authors.forename: D D
authors.initials: DD
authors.lastname: Yu
authors.affiliation: Department of Joint Surgery, Zhejiang Provincial Corps Hospital, Chinese People's Armed Police Forces, JiaXing, China.
authors.forename: J R
authors.initials: JR
authors.lastname: Lu
authors.affiliation: Department of Joint Surgery, Zhejiang Provincial Corps Hospital, Chinese People's Armed Police Forces, JiaXing, China.
authors.forename: C
authors.initials: C
authors.lastname: Wu
authors.affiliation: Department of Joint Surgery, Zhejiang Provincial Corps Hospital, Chinese People's Armed Police Forces, JiaXing, China.
authors.forename: W X
authors.initials: WX
authors.lastname: Jin
mesh_terms: D000293:Adolescent; D000328:Adult; D001859:Bone Neoplasms / Q000175:diagnosis* / Q000235:genetics; D002648:Child; D005260:Female; D006801:Humans; D008297:Male; D012516:Osteosarcoma / Q000175:diagnosis* / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*; D055815:Young Adult
chemical_list: C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A