title: Abscisic acid deficiency increases defence responses against Myzus persicae in Arabidopsis.
abstract: Comparison of Arabidopsis thaliana (Arabidopsis) gene expression induced by Myzus persicae (green peach aphid) feeding, aphid saliva infiltration and abscisic acid (ABA) treatment showed a significant positive correlation. In particular, ABA-regulated genes are over-represented among genes that are induced by M. persicae saliva infiltration into Arabidopsis leaves. This suggests that the induction of ABA-related gene expression could be an important component of the Arabidopsis-aphid interaction. Consistent with this hypothesis, M. persicae populations induced ABA production in wild-type plants. Furthermore, aphid populations were smaller on Arabidopsis aba1-1 mutants, which cannot synthesize ABA, and showed a significant preference for wild-type plants compared with the mutant. Total free amino acids, which play an important role in aphid nutrition, were not altered in the aba1-1 mutant line, but the levels of isoleucine (Ile) and tryptophan (Trp) were differentially affected by aphids in wild-type and mutant plants. Recently, indole glucosinolates have been shown to promote aphid resistance in Arabidopsis. In this study, 4-methoxyindol-3-ylmethylglucosinolate was more abundant in the aba1-1 mutant than in wild-type Arabidopsis, suggesting that the induction of ABA signals that decrease the accumulation of defence compounds may be beneficial for aphids.
authors.affiliation: Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA, 50011, USA.
authors.forename: Melissa S
authors.initials: MS
authors.lastname: Hillwig
authors.affiliation: Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA, 50011, USA.
authors.forename: Mariana
authors.initials: M
authors.lastname: Chiozza
authors.affiliation: Boyce Thompson Institute for Plant Research, Ithaca, NY, 14853, USA.
authors.forename: Clare L
authors.initials: CL
authors.lastname: Casteel
authors.affiliation: Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA, 50011, USA.
authors.forename: Siau Ting
authors.initials: ST
authors.lastname: Lau
authors.affiliation: Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA, 50011, USA.
authors.forename: Jessica
authors.initials: J
authors.lastname: Hohenstein
authors.affiliation: Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA, 50011, USA.
authors.forename: Enrique
authors.initials: E
authors.lastname: Hernández
authors.affiliation: Boyce Thompson Institute for Plant Research, Ithaca, NY, 14853, USA.
authors.forename: Georg
authors.initials: G
authors.lastname: Jander
authors.affiliation: Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA, 50011, USA.
authors.forename: Gustavo C
authors.initials: GC
authors.lastname: MacIntosh
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001042:Aphids / Q000502:physiology*; D017360:Arabidopsis / Q000276:immunology* / Q000469:parasitology*; D060146:Ecotype; D005961:Glucosinolates / Q000378:metabolism; D060434:Herbivory / Q000502:physiology; D007211:Indoles / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D012463:Saliva
chemical_list: D000596:Amino Acids; D005961:Glucosinolates; D007211:Indoles; D000040:Abscisic Acid; C030374:indole
keywords: Arabidopsis; Myzus persicae; abscisic acid; amino acids; aphid choice; glucosinolates