title: Prevalence of Helicobacter pylori vacA, cagA, and iceA Genotypes in Cuban Patients with Upper Gastrointestinal Diseases.
abstract: Virulence factors of Helicobacter pylori can predict the development of different gastroduodenal diseases. There are scarce reports in Cuba about H. pylori isolates genotyping. The aim of the present investigation was to identify allelic variation of the virulence genes vacA, cagA, and iceA in sixty-eight patients diagnosed as H. pylori positive by culture. In seven out of 68 patients, strains from both gastric regions were obtained and considered independent. DNA was extracted from all the H. pylori strains and evaluated by PCR-genotyping. The vacA s1 allele, cagA gene, and iceA2 allele were the most prevalent (72.0%, 56.0%, and 57.3%, respectively). Alleles from m-region showed a similar frequency as s1a and s1b subtypes. The presence of multiple H. pylori genotypes in a single biopsy and two gastric region specimens were found. Significant statistical association was observed between iceA2 allele and patients with non-peptic ulcer dyspepsia (NUD) (P = 0.037) as well as virulence genotypes (s1, s1m2) and patients over 40 years old (P < 0.05). In conclusion, the results demonstrated a high prevalence of H. pylori virulent genotypes in Cuban patients over 40 years old while iceA2 alleles demonstrated a good specificity in patients with NUD.
authors.affiliation: Microbiology and Clinical Branches, Tropical Medicine Institute "Pedro Kourí", Avenida Novia del Mediodía Km 6 1/2, P.O. Box 601, Marianao 13, Havana, Cuba.
authors.forename: Onelkis
authors.identifier: 0000-0002-3976-2147
authors.initials: O
authors.lastname: Feliciano
authors.affiliation: Microbiology and Clinical Branches, Tropical Medicine Institute "Pedro Kourí", Avenida Novia del Mediodía Km 6 1/2, P.O. Box 601, Marianao 13, Havana, Cuba.
authors.forename: Oderay
authors.initials: O
authors.lastname: Gutierrez
authors.affiliation: Microbiology and Clinical Branches, Tropical Medicine Institute "Pedro Kourí", Avenida Novia del Mediodía Km 6 1/2, P.O. Box 601, Marianao 13, Havana, Cuba.
authors.forename: Lidunka
authors.initials: L
authors.lastname: Valdés
authors.affiliation: Endoscopy Department, Pedro Borrás Hospital, Havana, Cuba.
authors.forename: Trini
authors.initials: T
authors.lastname: Fragoso
authors.affiliation: Microbiology and Clinical Branches, Tropical Medicine Institute "Pedro Kourí", Avenida Novia del Mediodía Km 6 1/2, P.O. Box 601, Marianao 13, Havana, Cuba.
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Calderin
authors.affiliation: Microbiology and Clinical Branches, Tropical Medicine Institute "Pedro Kourí", Avenida Novia del Mediodía Km 6 1/2, P.O. Box 601, Marianao 13, Havana, Cuba.
authors.forename: Antonio Eduardo
authors.initials: AE
authors.lastname: Valdes
authors.affiliation: Microbiology and Clinical Branches, Tropical Medicine Institute "Pedro Kourí", Avenida Novia del Mediodía Km 6 1/2, P.O. Box 601, Marianao 13, Havana, Cuba.
authors.forename: Rafael
authors.initials: R
authors.lastname: Llanes
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000942:Antigens, Bacterial / Q000235:genetics*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D002648:Child; D003462:Cuba; D005260:Female; D005767:Gastrointestinal Diseases / Q000453:epidemiology / Q000235:genetics* / Q000382:microbiology; D005838:Genotype; D016481:Helicobacter Infections / Q000453:epidemiology / Q000235:genetics* / Q000382:microbiology; D016480:Helicobacter pylori / Q000235:genetics / Q000472:pathogenicity; D006801:Humans; D008297:Male; D008875:Middle Aged
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori; C063113:ice nucleation protein