title: Overexpression of miR-145-5p inhibits proliferation of prostate cancer cells and reduces SOX2 expression.
abstract: We aimed to perform functional analysis of miR-145-5p in prostate cancer (PCa) cells and to identify targets of miR-145-5p for understanding its role in PCa pathogenesis. PC3, DU145, LNCaP PCa, and PNT1a nontumorigenic prostate cell lines were utilized for functional analysis of miR-145-5p. Its overexpression caused inhibition of proliferation through apoptosis and reduced migration in PCa cells. SOX2 expression was significantly decreased in both mRNA and protein level in miR-145-5p-overexpressed PCa cells. We proposed that miR-145-5p, being an important regulator of SOX2, carries a crucial role in PCa tumorigenesis.
authors.affiliation: 1Department of Medical Genetics, Cerrahpasa Medical School, Istanbul University, Istanbul, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Ozen
authors.forename: Omer Faruk
authors.initials: OF
authors.lastname: Karatas
authors.forename: Sukru
authors.initials: S
authors.lastname: Gulluoglu
authors.forename: Omer Faruk
authors.initials: OF
authors.lastname: Bayrak
authors.forename: Serhat
authors.initials: S
authors.lastname: Sevli
authors.forename: Esra
authors.initials: E
authors.lastname: Guzel
authors.forename: Isin D
authors.initials: ID
authors.lastname: Ekici
authors.forename: Turhan
authors.initials: T
authors.lastname: Caskurlu
authors.forename: Mustafa
authors.initials: M
authors.lastname: Solak
authors.forename: Chad J
authors.initials: CJ
authors.lastname: Creighton
authors.forename: Michael
authors.initials: M
authors.lastname: Ittmann
mesh_terms: D017209:Apoptosis / Q000502:physiology; D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000502:physiology; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis*; D020411:Oligonucleotide Array Sequence Analysis; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D060888:Real-Time Polymerase Chain Reaction; D055748:SOXB1 Transcription Factors / Q000096:biosynthesis*
chemical_list: C541010:MIRN145 microRNA, human; D035683:MicroRNAs; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors
keywords: Cancer stem cell; Prostate cancer; SOX2; Stem cell marker; hsa-miR-145–5p