title: Fiber-Optic SPR Immunosensors Tailored To Target Epithelial Cells through Membrane Receptors.
abstract: We report, for the first time, the use of a surface plasmon resonance (SPR) fiber-optic immunosensor for selective cellular detection through membrane protein targeting. The sensor architecture lies on gold-coated tilted fiber Bragg gratings (Au-coated TFBGs) photoimprinted in the fiber core via a laser technique. TFBGs operate in the near-infrared wavelength range at ∼1550 nm, yielding optical and SPR sensing characteristics that are advantageous for the analyses of cellular bindings and technical compatibility with relatively low-cost telecommunication-grade measurement devices. In this work, we take consider their numerous assets to figure out their ability to selectively detect intact epithelial cells as analytes in cell suspensions in the range of 2-5 × 10(6) cells mL(-1). For this, the probe was first thermally annealed to ensure a strong adhesion of the metallic coating to the fiber surface. Its surface was then functionalized with specific monoclonal antibodies via alkanethiol self-assembled monolayers (SAMs) against extracellular domain of epidermal growth factor receptors (EGFRs) and characterized by peak force tapping atomic force microscopy. A differential diagnosis has been demonstrated between two model systems. The developed immunosensors were able to monitor, in real time, the specific attachment of single intact cells in concentrations from 3 × 10(6) cells mL(-1). Such results confirm that the developed probe fits the lab-on-fiber technology and has the potential to be used as a disposable device for in situ and real-time clinical diagnosis.
authors.affiliation: †Research Institute for Bioscience, Proteomics and Microbiology Laboratory, University of Mons (UMONS), Avenue du Champ de Mars 6, 7000 Mons, Belgium.|‡Faculty of Engineering, Electromagnetism and Telecommunication Department, University of Mons (UMONS), Boulevard Dolez 31, 7000 Mons, Belgium.
authors.forename: Viera
authors.initials: V
authors.lastname: Malachovská
authors.affiliation: †Research Institute for Bioscience, Proteomics and Microbiology Laboratory, University of Mons (UMONS), Avenue du Champ de Mars 6, 7000 Mons, Belgium.|‡Faculty of Engineering, Electromagnetism and Telecommunication Department, University of Mons (UMONS), Boulevard Dolez 31, 7000 Mons, Belgium.
authors.forename: Clotilde
authors.initials: C
authors.lastname: Ribaut
authors.affiliation: ‡Faculty of Engineering, Electromagnetism and Telecommunication Department, University of Mons (UMONS), Boulevard Dolez 31, 7000 Mons, Belgium.
authors.forename: Valérie
authors.initials: V
authors.lastname: Voisin
authors.affiliation: §Research Institute for Materials Science and Engineering, Laboratory for Chemistry of Novel Materials, University of Mons (UMONS), Place du Parc 20, 7000 Mons, Belgium.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Surin
authors.affiliation: §Research Institute for Materials Science and Engineering, Laboratory for Chemistry of Novel Materials, University of Mons (UMONS), Place du Parc 20, 7000 Mons, Belgium.
authors.forename: Philippe
authors.initials: P
authors.lastname: Leclère
authors.affiliation: †Research Institute for Bioscience, Proteomics and Microbiology Laboratory, University of Mons (UMONS), Avenue du Champ de Mars 6, 7000 Mons, Belgium.
authors.forename: Ruddy
authors.initials: R
authors.lastname: Wattiez
authors.affiliation: ‡Faculty of Engineering, Electromagnetism and Telecommunication Department, University of Mons (UMONS), Boulevard Dolez 31, 7000 Mons, Belgium.
authors.forename: Christophe
authors.initials: C
authors.lastname: Caucheteur
mesh_terms: D015374:Biosensing Techniques; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000737:chemistry*; D066246:ErbB Receptors / Q000032:analysis*; D005336:Fiber Optic Technology; D006801:Humans; D018625:Microscopy, Atomic Force; D020349:Surface Plasmon Resonance
chemical_list: D066246:ErbB Receptors