title: Behavioral and biochemical responses in freshwater fish Carassius auratus exposed to sertraline.
abstract: Sertraline is one of the most commonly prescribed selective serotonin reuptake inhibitors and is frequently detected in the aquatic environment. However, knowledge regarding relationships among molecular or biochemical endpoints involved in modes of action (MOAs) of sertraline and ecologically important behavioral responses of fish is insufficient. The present study aimed to investigate the bioconcentration and possible adverse outcomes pathways (AOPs) in crucian carp (Carassius auratus) exposed to various concentrations of sertraline (4.36, 21.3 and 116 μg L(-1)) for 7 d. Bioconcentration factor values were in the range of 19.5-626 in liver, 6.94-285 in brain, 4.01-146 in gill and 0.625-43.1 in muscle during the entire period of exposure. Liver superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione-S-transferase (GST) activities and brain acetylcholinesterase (AChE) activity were selected as biochemical endpoints associated with MOAs. Swimming activity, shoaling, feeding rate and food consumption were determined to assess behavioral responses. Fish plasma levels of sertraline exceeding human therapeutic doses were also predicted from external exposure concentrations. Significant enhancements in CAT, GPx, AChE and swimming activities and decreases in shoaling tendency, feeding rate and food consumption were observed when fish plasma levels exceeded human therapeutic thresholds. Shoaling, feeding rate and food consumption were correlated with the activities of SOD, CAT and GST. A significant positive correlation between swimming activity and AChE activity was also observed. As such, our study provides important AOPs linking biochemical responses with ultimate ecologically relevant behavioral endpoints.
authors.affiliation: Key Laboratory of Integrated Regulation and Resources Development of Shallow Lakes of Ministry of Education, College of Environment, Hohai University, Nanjing 210098, China.
authors.forename: Zhengxin
authors.initials: Z
authors.lastname: Xie
authors.affiliation: Key Laboratory of Integrated Regulation and Resources Development of Shallow Lakes of Ministry of Education, College of Environment, Hohai University, Nanjing 210098, China. Electronic address: ghlu@hhu.edu.cn.
authors.forename: Guanghua
authors.initials: G
authors.lastname: Lu
authors.affiliation: Key Laboratory of Integrated Regulation and Resources Development of Shallow Lakes of Ministry of Education, College of Environment, Hohai University, Nanjing 210098, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Li
authors.affiliation: Key Laboratory of Integrated Regulation and Resources Development of Shallow Lakes of Ministry of Education, College of Environment, Hohai University, Nanjing 210098, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Nie
authors.affiliation: Key Laboratory of Integrated Regulation and Resources Development of Shallow Lakes of Ministry of Education, College of Environment, Hohai University, Nanjing 210098, China.
authors.forename: Binni
authors.initials: B
authors.lastname: Ma
authors.affiliation: Key Laboratory of Integrated Regulation and Resources Development of Shallow Lakes of Ministry of Education, College of Environment, Hohai University, Nanjing 210098, China.
authors.forename: Jianchao
authors.initials: J
authors.lastname: Liu
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D002347:Carps / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D005618:Fresh Water; D005880:Gills / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006054:Goldfish / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D008099:Liver / Q000378:metabolism; D017367:Selective Serotonin Reuptake Inhibitors / Q000378:metabolism; D020280:Sertraline / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*
chemical_list: D000110:Acetylcholinesterase; D002374:Catalase; D005979:Glutathione Peroxidase; D017367:Selective Serotonin Reuptake Inhibitors; D020280:Sertraline; D013482:Superoxide Dismutase; D014874:Water Pollutants, Chemical
keywords: Adverse outcomes pathways; Antioxidant enzymes; Behavior; Bioconcentration; Sertraline