title: Anti-Diabetic Effect of Aster sphathulifolius in C57BL/KsJ-db/db Mice.
abstract: In this study, we investigated the anti-diabetic effect of Aster sphathulifolius (AS) extract in C57BL/KsJ-db/db mice. The db/db mice were orally administered with AS 50% ethanol extract at concentrations of 50, 100, and 200 mg/kg/day (db/db-AS50, db/db-AS100, and db/db-AS200, respectively) for 10 weeks. Food and water intake, fasting blood glucose concentrations, blood glycosylated hemoglobin levels, and plasma insulin levels were significantly lower in the db/db-AS200 group than in the vehicle-treated db/db group; whereas glucose tolerance was significantly improved in the db/db-AS200 group. Moreover, AS dose dependently increased both insulin receptor substrate 1 and glucose transporter type 4 expression in skeletal muscle, significantly increased glucokinase expression, and decreased glucose 6-phosphatase and phosphoenolpyruvate carboxykinase expressions in the liver. The expressions of transcription factors, such as sterol-regulatory element-binding protein, peroxisome proliferator-activated receptor γ, and adipocyte protein 2, were upregulated in adipose tissue. Furthermore, immunohistochemical analysis showed that AS upregulated insulin production by increasing pancreatic β-cell mass. In summary, AS extract normalized hyperglycemia by multiple mechanisms: inhibition of glyconeogenesis, acceleration of glucose metabolism and lipid metabolism, and increase of glucose uptake. Using in vivo assays, this study has shown the potential of AS as a medicinal food and suggests the efficacy of AS for the use of prevention of diabetes.
authors.affiliation: 1 Department of Food Science and Nutrition, Hallym University , Gwangwon, Korea.
authors.forename: Xingfu
authors.initials: X
authors.lastname: Yin
authors.affiliation: 1 Department of Food Science and Nutrition, Hallym University , Gwangwon, Korea.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Huang
authors.affiliation: 1 Department of Food Science and Nutrition, Hallym University , Gwangwon, Korea.
authors.forename: Da-Woon
authors.initials: DW
authors.lastname: Jung
authors.affiliation: 2 Newtree Co., Ltd. , Gyeonggi, Korea.
authors.forename: Hee Chul
authors.initials: HC
authors.lastname: Chung
authors.affiliation: 3 Department of Preventive Pharmacy and Toxicology, College of Pharmacy, Kyung Hee University , Seoul, Korea.
authors.forename: Se Young
authors.initials: SY
authors.lastname: Choung
authors.affiliation: 1 Department of Food Science and Nutrition, Hallym University , Gwangwon, Korea.
authors.forename: Jae-Hoon
authors.initials: JH
authors.lastname: Shim
authors.affiliation: 1 Department of Food Science and Nutrition, Hallym University , Gwangwon, Korea.
authors.forename: Il-Jun
authors.initials: IJ
authors.lastname: Kang
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D031173:Aster Plant; D001786:Blood Glucose / Q000378:metabolism*; D050260:Carbohydrate Metabolism / Q000187:drug effects; D003920:Diabetes Mellitus / Q000097:blood / Q000517:prevention & control*; D002149:Energy Intake / Q000187:drug effects; D005941:Glucokinase / Q000097:blood; D005951:Glucose Tolerance Test; D005952:Glucose-6-Phosphatase / Q000097:blood; D006442:Glycated Hemoglobin / Q000378:metabolism; D006943:Hyperglycemia / Q000097:blood / Q000188:drug therapy*; D007004:Hypoglycemic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D007328:Insulin / Q000097:blood; D050417:Insulin-Secreting Cells / Q000187:drug effects; D050356:Lipid Metabolism / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008810:Mice, Inbred C57BL; D047495:PPAR gamma / Q000097:blood; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D001786:Blood Glucose; D005941:Glucokinase; D005952:Glucose-6-Phosphatase; D006442:Glycated Hemoglobin; D007004:Hypoglycemic Agents; D007328:Insulin; D047495:PPAR gamma; D010936:Plant Extracts
keywords: Aster sphathulifolius; C57BL/KsJ-db/db mice; blood glucose; diabetes mellitus; insulin resistance