title: Bis Expression in Patients with Surgically Resected Lung Cancer and its Clinical Significance.
abstract: BACKGROUND
  Bis, also known as BAG3, has been identified as a Bcl-2-interacting protein that enhances cellular anti-apoptotic activity. It is involved in cellular differentiation, angiogenesis, migration, and invasion in various tumors. The purpose of this study was to investigate the Bis expression pattern, and the clinical significance thereof, in patients with resected lung cancer.
  
  
  METHODS
  We studied 121 lung cancer patients who underwent curative surgical resection. Patient clinicopathological characteristics were reviewed retrospectively from medical records, including tumor recurrence and survival. The expression of Bis protein in lung cancer tissues was evaluated by immunohistochemical staining and was assessed using a four-tiered intensity score system (negative, weak, moderate, strong). Enhanced Bis expression at the periphery of a tumor facing the adjacent nontumor region was referred as "marginal activity."
  
  
  RESULTS
  Although Bis expression was higher in squamous cell carcinoma than in adenocarcinoma, marginal activity was higher in adenocarcinoma than in squamous cell carcinoma. All of the small cell carcinomas and lung cancer with neuroendocrine differentiation examined were negative for Bis expression. Compared with stage I lung cancer, patients with stage II and IIIA lung cancer exhibited higher Bis protein levels in lung tissues. Recurrence and survival rates did not differ significantly according to Bis expression intensity score or marginal activity.
  
  
  CONCLUSIONS
  Our study demonstrated that Bis expression differed according to the histological type and pathological stage of the lung cancer. Further studies are needed to assess its use as a biomarker and its role in the molecular pathogenesis of lung cancer.
authors.affiliation: Division of Pulmonology, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Chang Dong
authors.initials: CD
authors.lastname: Yeo
authors.affiliation: Department of Hospital Pathology, The Catholic University of Korea, Seoul, Republic of Korea.|The Cancer Research Institute, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Gyeong Sin
authors.initials: GS
authors.lastname: Park
authors.affiliation: Division of Pulmonology, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.|The Cancer Research Institute, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Nahyeon
authors.initials: N
authors.lastname: Kang
authors.affiliation: Division of Pulmonology, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.|The Cancer Research Institute, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Su Yeon
authors.initials: SY
authors.lastname: Choi
authors.affiliation: The Cancer Research Institute, The Catholic University of Korea, Seoul, Republic of Korea.|Department of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.|Cancer Evolution Research Center, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Hye Yun
authors.initials: HY
authors.lastname: Kim
authors.affiliation: Department of Radiation Oncology, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Dong Soo
authors.initials: DS
authors.lastname: Lee
authors.affiliation: Department of Radiation Oncology, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Yeon Sil
authors.initials: YS
authors.lastname: Kim
authors.affiliation: Division of Pulmonology, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Young Kyoon
authors.initials: YK
authors.lastname: Kim
authors.affiliation: Department of Thoracic Surgery, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Jae Gil
authors.initials: JG
authors.lastname: Park
authors.affiliation: Department of Thoracic Surgery, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Sook Whan
authors.initials: SW
authors.lastname: Sung
authors.affiliation: Department of Hospital Pathology, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Kyo Young
authors.initials: KY
authors.lastname: Lee
authors.affiliation: Department of Biostatistics, The Catholic University of Korea, Seoul, Republic of Korea.|Clinical Research Coordinating Center, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Mi Sun
authors.initials: MS
authors.lastname: Park
authors.affiliation: Department of Biostatistics, The Catholic University of Korea, Seoul, Republic of Korea.|Clinical Research Coordinating Center, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Hyeon Woo
authors.initials: HW
authors.lastname: Yim
authors.affiliation: Division of Pulmonology, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea. cmcksj@catholic.ac.kr.|The Cancer Research Institute, The Catholic University of Korea, Seoul, Republic of Korea. cmcksj@catholic.ac.kr.
authors.forename: Seung Joon
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: The Cancer Research Institute, The Catholic University of Korea, Seoul, Republic of Korea. leejh@catholic.ac.kr.|Department of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea. leejh@catholic.ac.kr.|Cancer Evolution Research Center, The Catholic University of Korea, Seoul, Republic of Korea. leejh@catholic.ac.kr.
authors.forename: Jeong-Hwa
authors.initials: JH
authors.lastname: Lee
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D000368:Aged; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D018288:Carcinoma, Small Cell / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007124:Immunoenzyme Techniques; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D012189:Retrospective Studies
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; C414431:BAG3 protein, human; D014408:Biomarkers, Tumor