title: Genome sequence of a novel endornavirus from the phytopathogenic fungus Alternaria brassicicola.
abstract: In an effort to discover new mycoviruses from phytopathogenic fungi, a dsRNA molecule of 10,290 nt, resembling those associated with the viruses belonging to the family Endornaviridae, was isolated from Alternaria brassicicola, one of the causal agents of rapeseed black spot disease. Genome analysis revealed the presence of a single open reading frame coding for a polyprotein of 3400 aa containing conserved viral methyltransferase (MTR), viral RNA helicase 1 (Hel-1), and RNA-dependent RNA polymerase (RdRp) domains. In addition, a cysteine-rich region (CRR) with conserved CXCC motifs, shared among several endornaviruses, was also identified between the MTR and Hel-1 domains. Phylogenetic analysis based on the RdRp sequence strongly suggested that the virus infecting A. brassicicola should be considered a representative of a novel endornavirus species, and this virus was designated as Alternaria brassicicola endornavirus 1 (AbEV1).
authors.affiliation: Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Nongda Road 1, Furong District, Changsha, 410128, China.
authors.forename: Hong-Hong
authors.initials: HH
authors.lastname: Shang
authors.forename: Jie
authors.initials: J
authors.lastname: Zhong
authors.forename: Ru-Jia
authors.initials: RJ
authors.lastname: Zhang
authors.forename: Chuan-Yuan
authors.initials: CY
authors.lastname: Chen
authors.forename: Bi-Da
authors.initials: BD
authors.lastname: Gao
authors.forename: Hong-Jian
authors.initials: HJ
authors.lastname: Zhu
mesh_terms: D000528:Alternaria / Q000821:virology*; D000595:Amino Acid Sequence; D001483:Base Sequence; D029741:Brassica rapa / Q000382:microbiology; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D012328:RNA Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016415:Sequence Alignment; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D014764:Viral Proteins