title: A novel liquid/liquid extraction process composed of surfactant and acetonitrile for purification of polygalacturonase enzyme from Durio zibethinus.
abstract: Polygalacturonase is one of the important enzymes used in various industries such as food, detergent, pharmaceutical, textile, pulp and paper. A novel liquid/liquid extraction process composed of surfactant and acetonitrile was employed for the first time to purify polygalacturonase from Durio zibethinus. The influences of different parameters such as type and concentration of surfactants, concentrations of acetonitrile and composition of surfactant/acetonitrile on partitioning behavior and recovery of polygalacturonase was investigated. Moreover, the effect of pH of system and crude load on purification fold and yield of purified polygalacturonase were studied. The results of the experiment indicated the polygalacturonase was partitioned into surfactant top rich phase with impurities being partitioned into acetonitrile bottom rich phase in the novel method of liquid/liquid process composed of 23% (w/w) Triton X-100 and 19% (w/w) acetonitrile, at 55.6% of TLL (tie line length) crude load of 25% (w/w) at pH 6.0. Recovery and recycling of components also was measured in each successive step of liquid/liquid extraction process. The enzyme was successfully recovered by the method with a high purification factor of 14.3 and yield of 97.3% while phase components were also recovered and recycled above 95%. This study demonstrated that the novel method of liquid/liquid extraction process can be used as an efficient and economical extraction method rather than the traditional methods of extraction for the purification and recovery of the valuable enzyme.
authors.affiliation: Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. Electronic address: Mehrnoush@upm.edu.my.
authors.forename: Mehrnoush
authors.initials: M
authors.lastname: Amid
authors.affiliation: Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Halal Products Research Institute, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia.
authors.forename: Yazid
authors.initials: Y
authors.lastname: Manap
authors.affiliation: Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Farhana
authors.initials: F
authors.lastname: Azmira
authors.affiliation: Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Muhaini
authors.initials: M
authors.lastname: Hussin
authors.affiliation: Department of Pharmaceutical Technology, Faculty of Pharmacy, International Islamic University Malaysia, 25200 Kuantan, Pahang, Malaysia.
authors.forename: Zaidul Islam
authors.initials: ZI
authors.lastname: Sarker
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D035161:Bombacaceae / Q000737:chemistry / Q000201:enzymology*; D059625:Liquid-Liquid Extraction / Q000379:methods*; D010940:Plant Proteins / Q000302:isolation & purification*; D011096:Polygalacturonase / Q000302:isolation & purification*; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D000097:Acetonitriles; D010940:Plant Proteins; D013501:Surface-Active Agents; D011096:Polygalacturonase; C032159:acetonitrile
keywords: Acetonitrile; Durio zibethinus; Novel liquid/liquid extraction process; Polygalacturonase; Recycling of phase components; Surfactant