title: Anti-Inflammatory Activity in the Low Molecular Weight Fraction of Commercial Human Serum Albumin (LMWF5A).
abstract: The innate immune system is increasingly being recognized as a critical component in osteoarthritis (OA) pathophysiology. An ex vivo immunoassay utilizing human peripheral blood mononuclear cells (PBMC) was developed in order to assess the OA anti-inflammatory properties of the low molecular weight fraction (<5 kDa) of commercial human serum albumin (LMWF5A). PBMC from various donors were pre-incubated with LMWF5A before LPS stimulation. TNFα release was measured by ELISA in supernatants after an overnight incubation. A ≥ 30% decrease in TNFα release was observed. This anti-inflammatory effect is potentially useful in assessing potency of LMWF5A for the treatment of OA.
authors.affiliation: a Swedish Medical Center , Trauma Research Department , Englewood , Colorado , USA.|b St. Anthony Hospital , Lakewood , Colorado , USA.|c The Medical Center of Plano , Plano , Texas , USA.
authors.forename: Gregory W
authors.initials: GW
authors.lastname: Thomas
authors.affiliation: a Swedish Medical Center , Trauma Research Department , Englewood , Colorado , USA.|b St. Anthony Hospital , Lakewood , Colorado , USA.|c The Medical Center of Plano , Plano , Texas , USA.
authors.forename: Leonard T
authors.initials: LT
authors.lastname: Rael
authors.affiliation: b St. Anthony Hospital , Lakewood , Colorado , USA.|d Rocky Vista University , Parker , Colorado , USA.
authors.forename: Charles W
authors.initials: CW
authors.lastname: Mains
authors.affiliation: a Swedish Medical Center , Trauma Research Department , Englewood , Colorado , USA.|d Rocky Vista University , Parker , Colorado , USA.
authors.forename: Denetta
authors.initials: D
authors.lastname: Slone
authors.affiliation: c The Medical Center of Plano , Plano , Texas , USA.
authors.forename: Matthew M
authors.initials: MM
authors.lastname: Carrick
authors.affiliation: a Swedish Medical Center , Trauma Research Department , Englewood , Colorado , USA.|b St. Anthony Hospital , Lakewood , Colorado , USA.|c The Medical Center of Plano , Plano , Texas , USA.
authors.forename: Raphael
authors.initials: R
authors.lastname: Bar-Or
authors.affiliation: a Swedish Medical Center , Trauma Research Department , Englewood , Colorado , USA.|b St. Anthony Hospital , Lakewood , Colorado , USA.|c The Medical Center of Plano , Plano , Texas , USA.|d Rocky Vista University , Parker , Colorado , USA.
authors.forename: David
authors.initials: D
authors.lastname: Bar-Or
mesh_terms: D003907:Dexamethasone / Q000494:pharmacology; D006801:Humans; D007249:Inflammation / Q000276:immunology / Q000473:pathology / Q000517:prevention & control; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000494:pharmacology; D015735:Mifepristone / Q000494:pharmacology; D008970:Molecular Weight; D061251:Primary Cell Culture; D012709:Serum Albumin / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D008070:Lipopolysaccharides; D012709:Serum Albumin; D014409:Tumor Necrosis Factor-alpha; D015735:Mifepristone; D003907:Dexamethasone
keywords: human serum albumin; lipopolysaccharide; osteoarthritis; peripheral blood mononuclear cells; tumor necrosis factor α