title: Activation of Adenosine Monophosphate-Activated Protein Kinase Is an Additional Mechanism That Participates in Mediating Inhibitory Actions of Prostaglandin F2Alpha in Mature, but Not Developing, Bovine Corpora Lutea.
abstract: Elevated cytosolic calcium and protein kinase C are well-established mediators of luteolytic actions of prostaglandin F2alpha (PGF2alpha). The objectives of this study were to determine 1) if calcium/calmodulin-dependent kinase kinase 2 (CAMKK2) participates in mediating PGF2alpha actions in developing (Day [d]-4) and mature (d-10) bovine corpus luteum (CL), 2) distal targets of CAMKK2, 3) developmental expression of adenosine monophosphate-activated protein kinase (AMPK), and 4) effects of AMPK activation on progesterone (P4) production. Expression of AMPK increased as the CL matured. Activation of the prostaglandin receptor (FP) induced rapid phosphorylation of AMPK, which was blocked by a CAMKK2 inhibitor. Changes in basal P4 secretion in vitro were determined in response to AMPK activation via metformin (met) or 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) in d-4 and d-10 CL. Production of P4 in d-10 CL decreased with met or AICAR compared to control, similar to activation by PGF2alpha. Therefore, potential distal targets of AMPK in d-10 CL were examined during induced functional regression via exogenous PGF2alpha. Serum and luteal P4 decreased at 2 and 4 h after administration of PGF2alpha. Protein expression of LDLR decreased at 2 and 4 h, while those of ACAT1 and STAR increased 4 h after PGF2alpha. During induced regression, alterations of cholesterol transport proteins contributed to decreased luteal and serum P4. Therefore, developmental differences in signal transduction associated with FP, specifically CAMKK2 and AMPK, partially contribute to differences in the ability of PGF2alpha to induce regression in mature, but not developing, bovine CL. Multiple cholesterol transport proteins, including LDLR, were altered by PGF2alpha and could be potential AMPK targets.
authors.affiliation: Animal and Nutritional Sciences, West Virginia University, Morgantown, West Virginia.
authors.forename: Elizabeth C
authors.initials: EC
authors.lastname: Bowdridge
authors.affiliation: Department of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, Kansas.
authors.forename: Madhusudan P
authors.initials: MP
authors.lastname: Goravanahally
authors.affiliation: Animal and Nutritional Sciences, West Virginia University, Morgantown, West Virginia.
authors.forename: E Keith
authors.initials: EK
authors.lastname: Inskeep
authors.affiliation: Department of Biology, West Virginia University, Morgantown, West Virginia jflores@wvu.edu.
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Flores
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D000620:Aminoimidazole Carboxamide / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D001562:Benzimidazoles / Q000494:pharmacology; D054737:Calcium-Calmodulin-Dependent Protein Kinase Kinase / Q000378:metabolism; D002417:Cattle; D003338:Corpus Luteum / Q000187:drug effects / Q000378:metabolism*; D015237:Dinoprost / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D053644:Naphthalimides / Q000494:pharmacology; D010766:Phosphorylation; D011374:Progesterone / Q000378:metabolism; D012265:Ribonucleotides / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D001562:Benzimidazoles; D004791:Enzyme Inhibitors; D053644:Naphthalimides; D012265:Ribonucleotides; C458525:STO 609; D000620:Aminoimidazole Carboxamide; D011374:Progesterone; D015237:Dinoprost; D054737:Calcium-Calmodulin-Dependent Protein Kinase Kinase; C517702:Camkk2 protein, rat; D055372:AMP-Activated Protein Kinases; C031143:AICA ribonucleotide
keywords: AMPK; bovine; corpus luteum; luteal regression; luteolysis; mechanisms of hormone action; progesterone; prostaglandins; protein kinases