title: [Effects of basic orange II on proliferation and differentiation of limb bud cells in rat embryos].
abstract: OBJECTIVE
  To explore the effects of basic orange II on proliferation and differentiation of limb bud cells.
  
  
  METHODS
  Limb bud cell were separated from SD rat embryo at 13-day gestational age, limb bud cell were exposed to basic orange II at concentrations of 0.0, 12.5, 25.0, 50.0, 100.0, 200, 0 and 400.0 mg/L in the culture medium. The effect of basic orange II on limb bud cell proliferation was detected by Cell Counting Kit-8, the effect of basic orange II on limb bud cell differentiation was assessed by Alcian Blue 8GX.
  
  
  RESULTS
  With the increasing of basic orange II concentration, the proliferation and differentiation of embryo limb bud cells were poorer and poorer in vitro, and there was the dose-effect relationship. The pID50 and dLD50 of basic orange II on limb bud cells were 240.6 mg/L and 69.3 mg/L respectively. The inhibition of basic orange II on cell differentiation might exceed that on cell proliferation.
  
  
  CONCLUSIONS
  Basic orange II could inhibit proliferation and differentiation of embryo limb bud cells. It might be a potential developmental toxic substance in rat embryo.
authors.forename: Lixin
authors.initials: L
authors.lastname: Zheng
authors.forename: Jiawang
authors.initials: J
authors.lastname: Feng
authors.forename: Shimin
authors.initials: S
authors.lastname: Tian
mesh_terms: D000818:Animals; D001391:Azo Compounds / Q000633:toxicity*; D001557:Benzenesulfonates / Q000633:toxicity*; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D018878:Limb Buds / Q000166:cytology / Q000187:drug effects* / Q000196:embryology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D001391:Azo Compounds; D001557:Benzenesulfonates; C014722:2-naphthol orange