title: The gene expression profile of resistant and susceptible Bombyx mori strains reveals cypovirus-associated variations in host gene transcript levels.
abstract: High-throughput paired-end RNA sequencing (RNA-Seq) was performed to investigate the gene expression profile of a susceptible Bombyx mori strain, Lan5, and a resistant B. mori strain, Ou17, which were both orally infected with B. mori cypovirus (BmCPV) in the midgut. There were 330 and 218 up-regulated genes, while there were 147 and 260 down-regulated genes in the Lan5 and Ou17 strains, respectively. Gene ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment for differentially expressed genes (DEGs) were carried out. Moreover, gene interaction network (STRING) analyses were performed to analyze the relationships among the shared DEGs. Some of these genes were related and formed a large network, in which the genes for B. mori cuticular protein RR-2 motif 123 (BmCPR123) and the gene for B. mori DNA replication licensing factor Mcm2-like (BmMCM2) were key genes among the common up-regulated DEGs, whereas the gene for B. mori heat shock protein 20.1 (Bmhsp20.1) was the central gene among the shared down-regulated DEGs between Lan5 vs Lan5-CPV and Ou17 vs Ou17-CPV. These findings established a comprehensive database of genes that are differentially expressed in response to BmCPV infection between silkworm strains that differed in resistance to BmCPV and implied that these DEGs might be involved in B. mori immune responses against BmCPV infection.
authors.affiliation: School of Biology and Basic Medical Science, Soochow University, Suzhou, 215123, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Guo
authors.forename: Simei
authors.initials: S
authors.lastname: Wang
authors.forename: Renyu
authors.initials: R
authors.lastname: Xue
authors.forename: Guangli
authors.initials: G
authors.lastname: Cao
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Hu
authors.forename: Moli
authors.initials: M
authors.lastname: Huang
authors.forename: Yangqi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yahong
authors.initials: Y
authors.lastname: Lu
authors.forename: Liyuan
authors.initials: L
authors.lastname: Zhu
authors.forename: Fei
authors.initials: F
authors.lastname: Chen
authors.forename: Zi
authors.initials: Z
authors.lastname: Liang
authors.forename: Sulan
authors.initials: S
authors.lastname: Kuang
authors.forename: Chengliang
authors.initials: C
authors.lastname: Gong
mesh_terms: D000818:Animals; D012831:Bombyx / Q000235:genetics* / Q000276:immunology / Q000821:virology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D054884:Host-Pathogen Interactions; D019476:Insect Proteins / Q000235:genetics* / Q000276:immunology; D012087:Reoviridae / Q000235:genetics / Q000502:physiology*
chemical_list: D019476:Insect Proteins