title: The Petunia GRAS Transcription Factor ATA/RAM1 Regulates Symbiotic Gene Expression and Fungal Morphogenesis in Arbuscular Mycorrhiza.
abstract: Arbuscular mycorrhiza (AM) is a mutual symbiosis that involves a complex symbiotic interface over which nutrients are exchanged between the plant host and the AM fungus. Dozens of genes in the host are required for the establishment and functioning of the interaction, among them nutrient transporters that mediate the uptake of mineral nutrients delivered by the fungal arbuscules. We have isolated in a genetic mutant screen a petunia (Petunia hybrida) Gibberellic Acid Insensitive, Repressor of Gibberellic Acid Insensitive, and Scarecrow (GRAS)-type transcription factor, Atypical Arbuscule (ATA), that acts as the central regulator of AM-related genes and is required for the morphogenesis of arbuscules. Forced mycorrhizal inoculations from neighboring wild-type plants revealed an additional role of ATA in restricting mycorrhizal colonization of the root meristem. The lack of ATA, which represents the ortholog of Required For Arbuscular Mycorrhiza1 in Medicago truncatula, renders the interaction completely ineffective, hence demonstrating the central role of AM-related genes for arbuscule development and function.
authors.affiliation: Department of Biology (M.K.R., M.S., L.B., L.F., D.R.), and Swiss Institute of Bioinformatics (L.F.), University of Fribourg, CH-1700 Fribourg, Switzerland; andDepartment of Reproduction and Plant Development, Centre National de la Recherche Scientifique/Institut National de la Recherche Agronomique/Ecole Normal Supérieure de Lyon, F-69364 Lyon Cedex 07, France (P.M., M.V.).
authors.forename: Mélanie K
authors.initials: MK
authors.lastname: Rich
authors.affiliation: Department of Biology (M.K.R., M.S., L.B., L.F., D.R.), and Swiss Institute of Bioinformatics (L.F.), University of Fribourg, CH-1700 Fribourg, Switzerland; andDepartment of Reproduction and Plant Development, Centre National de la Recherche Scientifique/Institut National de la Recherche Agronomique/Ecole Normal Supérieure de Lyon, F-69364 Lyon Cedex 07, France (P.M., M.V.).
authors.forename: Martine
authors.identifier: http://orcid.org/0000-0002-6323-1979
authors.initials: M
authors.lastname: Schorderet
authors.affiliation: Department of Biology (M.K.R., M.S., L.B., L.F., D.R.), and Swiss Institute of Bioinformatics (L.F.), University of Fribourg, CH-1700 Fribourg, Switzerland; andDepartment of Reproduction and Plant Development, Centre National de la Recherche Scientifique/Institut National de la Recherche Agronomique/Ecole Normal Supérieure de Lyon, F-69364 Lyon Cedex 07, France (P.M., M.V.).
authors.forename: Laure
authors.identifier: http://orcid.org/0000-0002-6681-7086
authors.initials: L
authors.lastname: Bapaume
authors.affiliation: Department of Biology (M.K.R., M.S., L.B., L.F., D.R.), and Swiss Institute of Bioinformatics (L.F.), University of Fribourg, CH-1700 Fribourg, Switzerland; andDepartment of Reproduction and Plant Development, Centre National de la Recherche Scientifique/Institut National de la Recherche Agronomique/Ecole Normal Supérieure de Lyon, F-69364 Lyon Cedex 07, France (P.M., M.V.).
authors.forename: Laurent
authors.identifier: http://orcid.org/0000-0001-8102-7579
authors.initials: L
authors.lastname: Falquet
authors.affiliation: Department of Biology (M.K.R., M.S., L.B., L.F., D.R.), and Swiss Institute of Bioinformatics (L.F.), University of Fribourg, CH-1700 Fribourg, Switzerland; andDepartment of Reproduction and Plant Development, Centre National de la Recherche Scientifique/Institut National de la Recherche Agronomique/Ecole Normal Supérieure de Lyon, F-69364 Lyon Cedex 07, France (P.M., M.V.).
authors.forename: Patrice
authors.identifier: http://orcid.org/0000-0003-1690-8032
authors.initials: P
authors.lastname: Morel
authors.affiliation: Department of Biology (M.K.R., M.S., L.B., L.F., D.R.), and Swiss Institute of Bioinformatics (L.F.), University of Fribourg, CH-1700 Fribourg, Switzerland; andDepartment of Reproduction and Plant Development, Centre National de la Recherche Scientifique/Institut National de la Recherche Agronomique/Ecole Normal Supérieure de Lyon, F-69364 Lyon Cedex 07, France (P.M., M.V.).
authors.forename: Michiel
authors.identifier: http://orcid.org/0000-0002-4192-7369
authors.initials: M
authors.lastname: Vandenbussche
authors.affiliation: Department of Biology (M.K.R., M.S., L.B., L.F., D.R.), and Swiss Institute of Bioinformatics (L.F.), University of Fribourg, CH-1700 Fribourg, Switzerland; andDepartment of Reproduction and Plant Development, Centre National de la Recherche Scientifique/Institut National de la Recherche Agronomique/Ecole Normal Supérieure de Lyon, F-69364 Lyon Cedex 07, France (P.M., M.V.) didier.reinhardt@unifr.ch.
authors.forename: Didier
authors.identifier: http://orcid.org/0000-0003-3495-6783
authors.initials: D
authors.lastname: Reinhardt
mesh_terms: D015169:Colony Count, Microbial; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D056426:Genetic Loci; D046913:Medicago truncatula / Q000235:genetics / Q000382:microbiology; D018519:Meristem / Q000235:genetics / Q000382:microbiology; D008969:Molecular Sequence Data; D009024:Morphogenesis; D009154:Mutation / Q000235:genetics; D038821:Mycorrhizae / Q000254:growth & development*; D032306:Petunia / Q000235:genetics* / Q000382:microbiology*; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D013559:Symbiosis / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors