title: A role of cell adhesion molecules and gelatinases in human serum-induced aggregation of human eyelid-derived stem cells in vitro.
abstract: Human serum (HS) has been reported to induce aggregation of human eyelid adipose-derived stem cells (HEACs) during high-density culture in vitro. The present study focused on the role of cell adhesion molecules and gelatinases during HS-induced aggregation of HEACs. HS-induced aggregation occurred between 9-15 days of culture. Cells aggregated by HS medium (HS-agg) showed stronger expression of α2, α2B, αX, and CEACAM1 genes compared to non-aggregated cells in HS medium (HS-ex) or in control FBS-cultured cells. HS-agg were distinctly labeled with antibodies against α2, α2B, and αX proteins. Western blot results demonstrated that the two integrin proteins were greatly expressed in HS-agg compared to HS-ex and control FBS-cultured cells. Treatment of HEACs with anti-integrin α2 antibody during culture in HS medium delayed aggregation formation. HS-agg exhibited strong expression of MMP1 and MMP9 compared to HS-ex or FBS-cultured cells. Conditioned media from HS-culture showed remarkable increase of MMP9 gelatinolytic activity in comparison to those from FBS-culture. However, there was no change of TIMP mRNA expression in relation to the HS-induced aggregation. Based on these results, it is suggested that integrin α2, α2B, and αX, and MMP9 might play an important role in the HS-induced aggregation of HEACs.
authors.affiliation: Department of Biotechnology, Seoul Women's University, Seoul 139-774, Republic of Korea.
authors.forename: Hyejin
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Biotechnology, Seoul Women's University, Seoul 139-774, Republic of Korea.
authors.forename: Yoon Hwa
authors.initials: YH
authors.lastname: Lim
authors.affiliation: Department of Biotechnology, Seoul Women's University, Seoul 139-774, Republic of Korea.
authors.forename: Sujin
authors.initials: S
authors.lastname: Yun
authors.affiliation: Department of Biotechnology, Seoul Women's University, Seoul 139-774, Republic of Korea.
authors.forename: A Young
authors.initials: AY
authors.lastname: Yoon
authors.affiliation: Department of Biotechnology, Seoul Women's University, Seoul 139-774, Republic of Korea.
authors.forename: Haekwon
authors.initials: H
authors.lastname: Kim
keywords: Aggregation; Human eyelid adipose-derived stem cells; Human serum; Integrin; MMP.