title: Nitric oxide mitigates arsenic-induced oxidative stress and genotoxicity in Vicia faba L.
abstract: The protective effects of nitric oxide (NO) against arsenic (As)-induced structural disturbances in Vicia faba have been investigated. As treatment (0.25, 0.50, and 1 mM) resulted in a declined growth of V. faba seedlings. Arsenic treatment stimulates the activity of SOD and CAT while the activities of APX and GST content were decreased. The oxidative stress markers such as superoxide radical, hydrogen peroxide and malondialdehyde (lipid peroxidation) contents were enhanced by As. Overall results revealed that significant accumulation of As suppressed growth, photosynthesis, antioxidant enzymes (SOD, CAT, APX, and GST activity), mitotic index, and induction of different chromosomal abnormalities, hence led to oxidative stress. The concentration of SNP (0.02 mM) was very effective in counteracting the adverse effect of As toxicity. These abnormalities use partially or fully reversed by a simultaneous application of As and NO donor and sodium nitroprusside and has an ameliorating effect against As-induced oxidative stress and genotoxicity in V. faba roots.
authors.affiliation: Department of Botany, Genotoxic Lab, Udai Pratap Autonomous College, Varanasi, 221002, India, shuklapratiksha20@gmail.com.
authors.forename: Pratiksha
authors.initials: P
authors.lastname: Shukla
authors.forename: A K
authors.initials: AK
authors.lastname: Singh
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D001151:Arsenic / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D004249:DNA Damage; D006861:Hydrogen Peroxide / Q000378:metabolism; D015227:Lipid Peroxidation; D009569:Nitric Oxide / Q000494:pharmacology*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology; D018384:Oxidative Stress; D010788:Photosynthesis / Q000187:drug effects; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D012989:Soil Pollutants / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D031307:Vicia faba / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D020030:Nitric Oxide Donors; D010940:Plant Proteins; D012989:Soil Pollutants; D009599:Nitroprusside; D009569:Nitric Oxide; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase; D001151:Arsenic