title: Suppression of microRNA-18a expression inhibits invasion and promotes apoptosis of human trophoblast cells by targeting the estrogen receptor α gene.
abstract: The purpose of the present study was to gain further understanding of the function of microRNA-18a (miR-18a) expression in the JEG-3 human trophoblast cell line. JEG-3 cells were transfected with pre-miR-18a mimics or miR-18a inhibitors. The effects of miR-18a on trophoblast cell invasion and apoptosis, and on the expression of estrogen receptor α (ESRα) were analyzed using a Transwell invasion assay, flow cytometry, reverse transcription-polymerase chain reaction, western blot analysis and a luciferase assay. The results of the present study suggested that miR-18a expression suppression led to a decrease in JEG-3 cell invasion and an increase in JEG-3 cell apoptosis, by inducing ESRα expression. The present study provides evidence for the involvement of miR-18a in the pathogenesis of pre-eclamptic pregnancies.
authors.affiliation: Department of Obstetrics and Gynecology, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shanxi 710038, P.R. China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Department of Obstetrics and Gynecology, Affiliated Hospital of Xi'an Medical University, Xi'an, Shanxi 710077, P.R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Orthopedics, Hainan Branch of PLA General Hospital, Sanya, Hainan 572013, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Han
authors.affiliation: Department of Obstetrics and Gynecology, General Hospital of Chinese People's Liberation Army, Beijing 100853, P.R. China.
authors.forename: Guowu
authors.initials: G
authors.lastname: Yin
authors.affiliation: Department of Obstetrics and Gynecology, General Hospital of Chinese People's Liberation Army, Beijing 100853, P.R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Gao
authors.affiliation: Department of Thoracic Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shanxi 710038, P.R. China.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Ni
authors.affiliation: Department of Obstetrics and Gynecology, General Hospital of Chinese People's Liberation Army, Beijing 100853, P.R. China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Su
authors.affiliation: Department of Obstetrics and Gynecology, General Hospital of Chinese People's Liberation Army, Beijing 100853, P.R. China.
authors.forename: Yuying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Obstetrics and Gynecology, General Hospital of Chinese People's Liberation Army, Beijing 100853, P.R. China.
authors.forename: Yuanqing
authors.initials: Y
authors.lastname: Yao
mesh_terms: D017209:Apoptosis; D002460:Cell Line; D002465:Cell Movement; D015536:Down-Regulation; D047628:Estrogen Receptor alpha / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D011225:Pre-Eclampsia / Q000235:genetics; D011247:Pregnancy; D014327:Trophoblasts / Q000166:cytology* / Q000378:metabolism
chemical_list: C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha; C540193:MIrn181 microRNA, human; D035683:MicroRNAs