title: Identification and quantification of anthocyanins in fruits from Neomitranthes obscura (DC.) N. Silveira an endemic specie from Brazil by comparison of chromatographic methodologies.
abstract: Neomitranthes obscura (DC.) N. Silveira is a Brazilian fruit belonging to the Myrtaceae family that contains anthocyanins in the peel and was studied for the first time in this work. Delphinidin-3-O-galactoside, delphinidin-3-O-glucoside, cyanidin-3-O-galactoside, cyanidin-3-O-glucoside, cyanidin-3-O-arabinoside, petunidin-3-O-glucoside, pelargonidin-3-O-glucoside, peonidin-3-O-galactoside, peonidin-3-O-glucoside, cyanidin-3-O-xyloside were separated and identified by LC/DAD/MS and by co-elution with standards. Reliable quantification of anthocyanins in the mature fruits was performed by HPLC/DAD using weighted linear regression model from 0.05 to 50mg of cyaniding-3-O-glucoside L(-1) because it gave better fit quality than least squares linear regression. Good precision and accuracy were obtained. The total anthocyanin content of mature fruits was 263.6 ± 8.2 mg of cyanidin-3-O-glucoside equivalents 100 g(-1) fresh weight, which was in the same range found in literature for anthocyanin rich fruits.
authors.affiliation: Universidade Federal Rural do Rio de Janeiro (UFRRJ) - Programa em Pós-Graduação em Ciência e Tecnologia de Alimentos, Rodovia BR 465 - km 7, Campus Universitário - Seropédica, 23851-970 Rio de Janeiro, RJ, Brazil.
authors.forename: Ana Cristina M S
authors.initials: AC
authors.lastname: Gouvêa
authors.affiliation: LAQV/REQUIMTE/Laboratório de Bromatologia e Hidrologia, Faculdade de Farmácia, Universidade do Porto, Rua Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal.
authors.forename: Armindo
authors.initials: A
authors.lastname: Melo
authors.affiliation: Embrapa Agroindústria de Alimentos, Laboratório de Cromatografia Líquida, Avenida das Américas 29501, 23020-470 Guaratiba, Rio de Janeiro, RJ, Brazil.
authors.forename: Manuela C P A
authors.initials: MC
authors.lastname: Santiago
authors.affiliation: Universidade Federal Rural do Rio de Janeiro (UFRRJ) - Programa em Pós-Graduação em Ciência e Tecnologia de Alimentos, Rodovia BR 465 - km 7, Campus Universitário - Seropédica, 23851-970 Rio de Janeiro, RJ, Brazil.
authors.forename: Fernanda M
authors.initials: FM
authors.lastname: Peixoto
authors.affiliation: LAQV/REQUIMTE/Chemistry Investigation Centre (CIQ), Department of Chemistry and Biochemistry, Faculty of Sciences, University of Porto, R. Campo Alegre 687, 4169-007 Porto, Portugal.
authors.forename: Vitor
authors.initials: V
authors.lastname: Freitas
authors.affiliation: Embrapa Agroindústria de Alimentos, Laboratório de Cromatografia Líquida, Avenida das Américas 29501, 23020-470 Guaratiba, Rio de Janeiro, RJ, Brazil.
authors.forename: Ronoel L O
authors.initials: RL
authors.lastname: Godoy
authors.affiliation: LAQV/REQUIMTE/Laboratório de Bromatologia e Hidrologia, Faculdade de Farmácia, Universidade do Porto, Rua Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal. Electronic address: isabel.ferreir@ff.up.pt.
authors.forename: Isabel M P L V O
authors.initials: IM
authors.lastname: Ferreira
mesh_terms: D000872:Anthocyanins / Q000032:analysis*; D001938:Brazil; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005638:Fruit / Q000737:chemistry*; D027822:Myrtaceae / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000872:Anthocyanins
keywords: Anthocyanins; HPLC/DAD/MS; Myrtaceae; Neomitranthes obscura