title: Hyaluronic acid/poly(lactic-co-glycolic acid) core/shell fiber meshes loaded with epigallocatechin-3-O-gallate as skin tissue engineering scaffolds.
abstract: In this study, hyaluronic acid (HA)/poly(lactic-co-glycolic acid, PLGA) core/shell fiber meshes loaded with epigallocatechin-3-O-gallate (EGCG) (HA/PLGA-E) for application to tissue engineering scaffolds for skin regeneration were prepared via coaxial electrospinning. Physicochemical properties of HA/PLGA-E core/shell fiber meshes were characterized by SEM, Raman spectroscopy, contact angle, EGCG release profiling and in vitro degradation. Biomechanical properties of HA/PLGA-E meshes were also investigated by a tensile strength test. SEM images showed that HA/PLGA-E fiber meshes had a three-dimensional interconnected pore structure with an average fiber diameter of about 1270 nm. Raman spectra revealed that EGCG was uniformly dispersed in the PLGA shell of meshes. HA/PLGA-E meshes showed sustained EGCG release patterns by controlled diffusion and PLGA degradation over 4 weeks. EGCG loading did not adversely affect the tensile strength and elastic modulus of HA/PLGA meshes, while increased their hydrophilicity and surface energy. Attachment of human dermal fibroblasts on HA/PLGA-E meshes was appreciably increased and their proliferation was steadily retained during the culture period. These results suggest that HA/PLGA-E core/shell fiber meshes can be potentially used as scaffolds supporting skin regeneration.
authors.forename: Eun Ji
authors.initials: EJ
authors.lastname: Lee
authors.forename: Jong Ho
authors.initials: JH
authors.lastname: Lee
authors.forename: Linhua
authors.initials: L
authors.lastname: Jin
authors.forename: Oh Seong
authors.initials: OS
authors.lastname: Jin
authors.forename: Yong Cheol
authors.initials: YC
authors.lastname: Shin
authors.forename: Jin Oh
authors.initials: JO
authors.lastname: Sang
authors.forename: Jaebeom
authors.initials: J
authors.lastname: Lee
authors.forename: Suong-Hyu
authors.initials: SH
authors.lastname: Hyon
authors.forename: Dong-Wook
authors.initials: DW
authors.lastname: Han
mesh_terms: D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D019344:Lactic Acid / Q000737:chemistry*; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D012867:Skin / Q000166:cytology*; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D002392:Catechin; D006820:Hyaluronic Acid; C057580:gallocatechol