title: [Tripeptides slow down aging process in renal cell culture].
abstract: The mechanism of geroprotective effect of peptides AED and EDL was studied in ageing renal cell culture. Peptide AED and EDL increase cell proliferation, decreasing expression of marker of aging p16, p21, p53 and increasing expression of SIRT-6 in young and aged renal cell culture. The reduction of SIRT-6 synthesis in cell is one of the causes of cell senescence. On the basis of experimental data models of interaction of peptides with various sites of DNA were constructed. Both peptides form most energetically favorable complexes with d(ATATATATAT)2 sequences in minor groove of DNA. It is shown that interaction of peptides AED and EDL with DNA is the cause of gene expression, encoded marker of ageing in renal cells.
authors.forename: V Kh
authors.initials: VKh
authors.lastname: Khavinson
authors.forename: S I
authors.initials: SI
authors.lastname: Tarnovskaia
authors.forename: N S
authors.initials: NS
authors.lastname: Lin'kova
authors.forename: V O
authors.initials: VO
authors.lastname: Poliakova
authors.forename: A O
authors.initials: AO
authors.lastname: Durnova
authors.forename: T E
authors.initials: TE
authors.lastname: Nichik
authors.forename: I M
authors.initials: IM
authors.lastname: Kvetnoĭ
authors.forename: M M
authors.initials: MM
authors.lastname: D'iakonov
authors.forename: P P
authors.initials: PP
authors.lastname: Iakutseni
mesh_terms: D000818:Animals; D001665:Binding Sites; D015415:Biomarkers / Q000378:metabolism; D002452:Cell Count; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects*; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics; D004247:DNA / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D007668:Kidney / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D062105:Molecular Docking Simulation; D009842:Oligopeptides / Q000494:pharmacology*; D061251:Primary Cell Culture; D017208:Rats, Wistar; D037761:Sirtuins / Q000235:genetics; D013997:Time Factors; D016159:Tumor Suppressor Protein p53 / Q000235:genetics
chemical_list: D015415:Biomarkers; D019941:Cyclin-Dependent Kinase Inhibitor p16; D050759:Cyclin-Dependent Kinase Inhibitor p21; D009842:Oligopeptides; D016159:Tumor Suppressor Protein p53; C572340:alanyl-glutamyl-aspartic acid; C000600952:glutamyl-aspartyl-leucine; D004247:DNA; D037761:Sirtuins; C560607:sirtuin 6, rat