title: Simultaneous determination of centchroman and tamoxifen along with their metabolites in rat plasma using LC-MS/MS.
abstract: OBJECTIVE
  Tamoxifen and centchroman are two non-steroidal, selective estrogen receptors modulators, intended for long term therapy in the woman. Because of their wide spread use, there is a possibility of co-prescription of these agents.
  
  
  METHODS
  We studied the probable pharmacokinetic interaction between these agents in breast cancer model rats. A simple, sensitive and rapid LC-ESI-MS/MS method was developed and validated for the simultaneous determination of tamoxifen, centchroman and their active metabolites.
  
  
  RESULTS
  The method was linear over a range of 0.2-200 ng/ml. All validation parameters met the acceptance criteria according to regulatory guidelines.
  
  
  CONCLUSIONS
  LC-MS/MS method for determination of tamoxifen, centchroman and their metabolites was developed and validated. Results show the potential of drug-drug interaction upon co-administration these two marketed drugs.
authors.affiliation: Academy of Scientific & Innovative Research, New Delhi, India.
authors.forename: Kanumuri Siva
authors.initials: KS
authors.lastname: Rama Raju
authors.forename: Isha
authors.initials: I
authors.lastname: Taneja
authors.forename: Sheelendra Pratap
authors.initials: SP
authors.lastname: Singh
authors.forename: Amit
authors.initials: A
authors.lastname: Tripathi
authors.forename: Durga Prasad
authors.initials: DP
authors.lastname: Mishra
authors.forename: K Mahaboob
authors.initials: KM
authors.lastname: Hussain
authors.forename: Jiaur Rahman
authors.initials: JR
authors.lastname: Gayen
authors.forename: Shio Kumar
authors.initials: SK
authors.lastname: Singh
authors.forename: Muhammad
authors.initials: M
authors.lastname: Wahajuddin
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity; D000818:Animals; D002273:Carcinogens / Q000633:toxicity; D002486:Centchroman / Q000097:blood* / Q000493:pharmacokinetics; D002853:Chromatography, Liquid / Q000379:methods*; D005260:Female; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced / Q000378:metabolism*; D055432:Metabolomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013629:Tamoxifen / Q000097:blood* / Q000493:pharmacokinetics; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014018:Tissue Distribution
chemical_list: D002273:Carcinogens; D013629:Tamoxifen; D002486:Centchroman; D015127:9,10-Dimethyl-1,2-benzanthracene