title: A nuclear role for the respiratory enzyme CLK-1 in regulating mitochondrial stress responses and longevity.
abstract: The coordinated regulation of mitochondrial and nuclear activities is essential for cellular respiration and its disruption leads to mitochondrial dysfunction, a hallmark of ageing. Mitochondria communicate with nuclei through retrograde signalling pathways that modulate nuclear gene expression to maintain mitochondrial homeostasis. The monooxygenase CLK-1 (human homologue COQ7) was previously reported to be mitochondrial, with a role in respiration and longevity. We have uncovered a distinct nuclear form of CLK-1 that independently regulates lifespan. Nuclear CLK-1 mediates a retrograde signalling pathway that is conserved from Caenorhabditis elegans to humans and is responsive to mitochondrial reactive oxygen species, thus acting as a barometer of oxidative metabolism. We show that, through modulation of gene expression, the pathway regulates both mitochondrial reactive oxygen species metabolism and the mitochondrial unfolded protein response. Our results demonstrate that a respiratory enzyme acts in the nucleus to control mitochondrial stress responses and longevity.
authors.forename: Richard M
authors.initials: RM
authors.lastname: Monaghan
authors.forename: Robert G
authors.initials: RG
authors.lastname: Barnes
authors.forename: Kate
authors.initials: K
authors.lastname: Fisher
authors.forename: Tereza
authors.initials: T
authors.lastname: Andreou
authors.forename: Nicholas
authors.initials: N
authors.lastname: Rooney
authors.forename: Gino B
authors.initials: GB
authors.lastname: Poulin
authors.forename: Alan J
authors.initials: AJ
authors.lastname: Whitmarsh
mesh_terms: D000375:Aging; D000818:Animals; D030801:Animals, Genetically Modified; D019556:COS Cells; D017173:Caenorhabditis elegans / Q000235:genetics; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019069:Cell Respiration; D002470:Cell Survival; D002522:Chlorocebus aethiops; D002843:Chromatin / Q000378:metabolism; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D008136:Longevity; D008928:Mitochondria / Q000378:metabolism*; D018384:Oxidative Stress; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction; D013312:Stress, Physiological; D056811:Unfolded Protein Response / Q000235:genetics
chemical_list: C104339:CLK-1 protein, C elegans; D029742:Caenorhabditis elegans Proteins; D002843:Chromatin; D017382:Reactive Oxygen Species; C066780:Clk dual-specificity kinases; D011505:Protein-Tyrosine Kinases; D017346:Protein Serine-Threonine Kinases