title: Identification of differentially expressed genes related to aphid resistance in cucumber (Cucumis sativus L.).
abstract: Cucumber, a very important vegetable crop worldwide, is easily damaged by pests. Aphids (Aphis gossypii Glover) are among the most serious pests in cucumber production and often cause severe loss of yield and make fruit quality get worse. Identifying genes that render cucumbers resistant to aphid-induced damage and breeding aphid-resistant cucumber varieties have become the most promising control strategies. In this study, a Illumina Genome Analyzer platform was applied to monitor changes in gene expression in the whole genome of the cucumber cultivar 'EP6392' which is resistant to aphids. Nine DGE libraries were constructed from infected and uninfected leaves. In total, 49 differentially expressed genes related to cucumber aphid resistance were screened during the treatment period. These genes are mainly associated with signal transduction, plant-pathogen interactions, flavonoid biosynthesis, amino acid metabolism and sugar metabolism pathways. Eight of the 49 genes may be associated with aphid resistance. Finally, expression of 9 randomly selected genes was evaluated by qRT-PCR to verify the results for the tag-mapped genes. With the exception of 1-aminocyclopropane-1-carboxylate oxidase homolog 6, the expression of the chosen genes was in agreement with the results of the tag-sequencing analysis patterns.
authors.affiliation: School of Horticulture and Plant Protection, Yangzhou University, Yangzhou, Jiangsu 225009, P.R. China.
authors.forename: Danna
authors.initials: D
authors.lastname: Liang
authors.affiliation: School of Horticulture and Plant Protection, Yangzhou University, Yangzhou, Jiangsu 225009, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: School of Horticulture and Plant Protection, Yangzhou University, Yangzhou, Jiangsu 225009, P.R. China.
authors.forename: Qijing
authors.initials: Q
authors.lastname: Hu
authors.affiliation: School of Horticulture and Plant Protection, Yangzhou University, Yangzhou, Jiangsu 225009, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: He
authors.affiliation: School of Horticulture and Plant Protection, Yangzhou University, Yangzhou, Jiangsu 225009, P.R. China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Qi
authors.affiliation: School of Horticulture and Plant Protection, Yangzhou University, Yangzhou, Jiangsu 225009, P.R. China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xu
authors.affiliation: School of Horticulture and Plant Protection, Yangzhou University, Yangzhou, Jiangsu 225009, P.R. China.
authors.forename: Fucai
authors.initials: F
authors.lastname: Zhou
authors.affiliation: School of Horticulture and Plant Protection, Yangzhou University, Yangzhou, Jiangsu 225009, P.R. China.
authors.forename: Xuehao
authors.initials: X
authors.lastname: Chen
mesh_terms: D000818:Animals; D001042:Aphids; D002874:Chromosome Mapping; D018553:Cucumis sativus / Q000235:genetics* / Q000469:parasitology*; D060467:Disease Resistance / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D010373:Lice Infestations