title: Transcriptome profiling of sugarcane roots in response to low potassium stress.
abstract: Sugarcane is the most important crop for supplying sugar. Due to its high biomass, sugarcane needs to absorb a large amount of potassium (K) throughout its lifecycle. In South China, a deficiency of K available in soil restricts the production of sugarcane. Increasing the tolerance of sugarcane to low-K will be an effective approach for improving survival of the crop in this area. However, there is little information regarding the mechanism of tolerance to low-K stress in sugarcane. In this study, a customized microarray was used to analyze the changes in the level of transcripts of sugarcane genes 8 h, 24 h and 72 h after exposure to low-K conditions. We identified a total of 4153 genes that were differentially expressed in at least one of the three time points. The number of genes responding to low-K stress at 72 h was almost 2-fold more than the numbers at 8 h and 24 h. Gene ontology (GO) analysis revealed that many genes involved in metabolic, developmental and biological regulatory processes displayed changes in the level of transcripts in response to low-K stress. Additionally, we detected differential expression of transcription factors, transporters, kinases, oxidative stress-related genes and genes in Ca+ and ethylene signaling pathways; these proteins might play crucial roles in improving the tolerance of sugarcane to low-K stress. The results of this study will help to better understand the molecular mechanisms of sugarcane tolerance to low-K.
authors.affiliation: Guangdong Key Lab of Sugarcane Improvement & Biorefinery, Guangzhou Sugarcane Industry Research Institute, Guangzhou, China.
authors.forename: Qiaoying
authors.initials: Q
authors.lastname: Zeng
authors.affiliation: Guangdong Key Lab of Sugarcane Improvement & Biorefinery, Guangzhou Sugarcane Industry Research Institute, Guangzhou, China.
authors.forename: Qiuping
authors.initials: Q
authors.lastname: Ling
authors.affiliation: Guangdong Key Lab of Sugarcane Improvement & Biorefinery, Guangzhou Sugarcane Industry Research Institute, Guangzhou, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Fan
authors.affiliation: Guangdong Key Lab of Sugarcane Improvement & Biorefinery, Guangzhou Sugarcane Industry Research Institute, Guangzhou, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
authors.affiliation: Guangdong Key Lab of Sugarcane Improvement & Biorefinery, Guangzhou Sugarcane Industry Research Institute, Guangzhou, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Hu
authors.affiliation: Guangdong Key Lab of Sugarcane Improvement & Biorefinery, Guangzhou Sugarcane Industry Research Institute, Guangzhou, China.
authors.forename: Jianwen
authors.initials: J
authors.lastname: Chen
authors.affiliation: Guangdong Key Lab of Sugarcane Improvement & Biorefinery, Guangzhou Sugarcane Industry Research Institute, Guangzhou, China.
authors.forename: Zhenrui
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Guangdong Key Lab of Sugarcane Improvement & Biorefinery, Guangzhou Sugarcane Industry Research Institute, Guangzhou, China.
authors.forename: Haihua
authors.initials: H
authors.lastname: Deng
authors.affiliation: Guangdong Key Lab of Sugarcane Improvement & Biorefinery, Guangzhou Sugarcane Industry Research Institute, Guangzhou, China.
authors.forename: Qiwei
authors.initials: Q
authors.lastname: Li
authors.affiliation: Guangdong Key Lab of Sugarcane Improvement & Biorefinery, Guangzhou Sugarcane Industry Research Institute, Guangzhou, China.
authors.forename: Yongwen
authors.initials: Y
authors.lastname: Qi
mesh_terms: D020869:Gene Expression Profiling / Q000379:methods*; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D010940:Plant Proteins / Q000235:genetics*; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development; D011188:Potassium / Q000378:metabolism*; D031786:Saccharum / Q000033:anatomy & histology / Q000235:genetics / Q000254:growth & development*; D013312:Stress, Physiological
chemical_list: D010940:Plant Proteins; D011188:Potassium