title: Single-Cell Characterization of in vitro Migration and Interaction Dynamics of T Cells Expanded with IL-2 and IL-7.
abstract: T cells are pivotal in the immune defense against cancers and infectious agents. To mount an effector response against cancer cells, T cells need to migrate to the cancer-site, engage in contacts with cancer cells, and perform their effector functions. Adoptive T cell therapy is an effective strategy as treatment of complications such as relapse or opportunistic infections after hematopoietic stem cell transplantations. This requires a sufficient amount of cells that are able to expand and respond to tumor or viral antigens. The cytokines interleukin (IL)-2 and IL-7 drive T cell differentiation, proliferation, and survival and are commonly used to expand T cells ex vivo. Here, we have used microchip-based live-cell imaging to follow the migration of individual T cells, their interactions with allogeneic monocytes, cell division, and apoptosis for extended periods of time; something that cannot be achieved by commonly used methods. Our data indicate that cells grown in IL-7 + IL-2 had similar migration and contact dynamics as cells grown in IL-2 alone. However, the addition of IL-7 decreased cell death creating a more viable cell population, which should be beneficial when preparing cells for immunotherapy.
authors.affiliation: Department of Laboratory Medicine, Division of Clinical Microbiology, Karolinska Institutet , Stockholm , Sweden.
authors.forename: Johanna
authors.initials: J
authors.lastname: Tauriainen
authors.affiliation: Science for Life Laboratory, Department of Applied Physics, KTH Royal Institute of Technology , Stockholm , Sweden.
authors.forename: Karin
authors.initials: K
authors.lastname: Gustafsson
authors.affiliation: Science for Life Laboratory, Department of Applied Physics, KTH Royal Institute of Technology , Stockholm , Sweden.
authors.forename: Mårten
authors.initials: M
authors.lastname: Göthlin
authors.affiliation: Center for Allogeneic Stem Cell Transplantation, Karolinska University Hospital Huddinge , Stockholm , Sweden ; Department of Oncology and Pathology, Karolinska Institutet , Stockholm , Sweden.
authors.forename: Jens
authors.initials: J
authors.lastname: Gertow
authors.affiliation: Department of Laboratory Medicine, Division of Clinical Microbiology, Karolinska Institutet , Stockholm , Sweden.
authors.forename: Marcus
authors.initials: M
authors.lastname: Buggert
authors.affiliation: Science for Life Laboratory, Department of Applied Physics, KTH Royal Institute of Technology , Stockholm , Sweden.
authors.forename: Thomas W
authors.initials: TW
authors.lastname: Frisk
authors.affiliation: Department of Laboratory Medicine, Division of Clinical Microbiology, Karolinska Institutet , Stockholm , Sweden.
authors.forename: Annika C
authors.initials: AC
authors.lastname: Karlsson
authors.affiliation: Center for Allogeneic Stem Cell Transplantation, Karolinska University Hospital Huddinge , Stockholm , Sweden ; Department of Oncology and Pathology, Karolinska Institutet , Stockholm , Sweden.
authors.forename: Michael
authors.initials: M
authors.lastname: Uhlin
authors.affiliation: Science for Life Laboratory, Department of Applied Physics, KTH Royal Institute of Technology , Stockholm , Sweden ; Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet , Stockholm , Sweden.
authors.forename: Björn
authors.initials: B
authors.lastname: Önfelt
keywords: IL-2; IL-7; T cell; fluorescence; live-cell imaging; microchip; microscopy; single-cell analysis