title: Role of miR-101 in pheochromocytoma patients with SDHD mutation.
abstract: This study aimed to screen the potential diagnostic biomarkers for distinguishing the malignant pheochromocytoma (PCC) from benign PCC. A total of 59 patients with PCC (benign and malignant) were enrolled in this study. The expression level of miRNAs in patients with different kind PCCs (healthy control, benign, malignant, malignant with or without SDHD mutation, adrenal and extra-adrenal) was analyzed using the qRT-PCR analysis. Besides, the diagnosis accuracy of miRNA in PCC samples was analyzed using the ROC analysis. Moreover, level of miR-101 in serum was detected by qRT-PCR analysis and serum VEGF level in patients with PCC was detected using the ELISA kit. Compared with benign PCC, miR-101 level was higher in patients with malignant PCC (P < 0.05), while the level of miR-513-5p and miR-26b showed no difference between malignant PCC and benign PCC (P > 0.05). miR-101 expression was significantly increased in malignant tumor tissue with SDHD mutation (P < 0.05) and in extra-adrenal tissues (P < 0.05), respectively. Besides, AUCs for miR-101 in PCC samples was 0.79 and for which in PCC samples with non-SDHD mutation was 0.77. Besides, serum miR-101 in malignant PCC was high but showed no difference among groups (P > 0.05). Moreover, serum VEGF level in malignant tumors was significantly high compared with benign tumor, as well as that in malignant PCC with SDHD mutation (P < 0.05). Our study suggested that SDHD mutation may enhance the overexpression of miR-101 in malignant tumors and miR-101 may be a potential diagnostic biomarker for malignant PCC and benign PCC.
authors.affiliation: Department of Cardiology, Binzhou People's Hospital Binzhou 256610, China.
authors.forename: Ligeng
authors.initials: L
authors.lastname: Zong
authors.affiliation: Department of Cardiology, Binzhou People's Hospital Binzhou 256610, China.
authors.forename: Lizhen
authors.initials: L
authors.lastname: Meng
authors.affiliation: Department of Endocrinology, GaoMi People's Hospital 77 Gaomi Town Mansion Street, Shandong 261500, China.
authors.forename: Ruhui
authors.initials: R
authors.lastname: Shi
mesh_terms: D000293:Adolescent; D000310:Adrenal Gland Neoplasms / Q000235:genetics* / Q000473:pathology; D000328:Adult; D000368:Aged; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009154:Mutation; D010673:Pheochromocytoma / Q000235:genetics* / Q000473:pathology; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction; D013385:Succinate Dehydrogenase / Q000235:genetics*; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; C534072:MIRN101 microRNA, human; D035683:MicroRNAs; C491545:SDHD protein, human; D013385:Succinate Dehydrogenase
keywords: Pheochromocytoma; SDHD; biomarker; diagnosis; miRNA