title: Fluorescent probe based on heteroatom containing styrylcyanine: pH-sensitive properties and bioimaging in vivo.
abstract: A novel fluorescent probe based on heteroatom containing styrylcyanine is synthesized. The fluorescence of probe is bright green in basic and neutral media but dark orange in strong acidic environments, which could be reversibly switched. Such behavior enables it to work as a fluorescent pH sensor in the solution state and a chemosensor for detecting acidic and basic volatile organic compounds. Analyses by NMR spectroscopy confirm that the protonation or deprotonation of pyridinyl moiety is responsible for the sensing process. In addition, the fluorescent microscopic images of probe in live cells and zebrafish are achieved successfully, suggesting that the probe has good cell membrane permeability and low cytotoxicity.
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510640, PR China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Yang
authors.affiliation: School of Basic Medical Science, Southern Medical University, Guangzhou 510515, PR China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Gao
authors.affiliation: School of Basic Medical Science, Southern Medical University, Guangzhou 510515, PR China.
authors.forename: Zhibing
authors.initials: Z
authors.lastname: Huang
authors.affiliation: School of Basic Medical Science, Southern Medical University, Guangzhou 510515, PR China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Chen
authors.affiliation: School of Basic Medical Science, Southern Medical University, Guangzhou 510515, PR China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Ke
authors.affiliation: Department of Organic Pharmaceutical Chemistry, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, PR China.
authors.forename: Peiliang
authors.initials: P
authors.lastname: Zhao
authors.affiliation: Department of Organic Pharmaceutical Chemistry, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, PR China.
authors.forename: Yichen
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Department of Organic Pharmaceutical Chemistry, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, PR China. Electronic address: ruiyliu@smu.edu.cn.
authors.forename: Ruiyuan
authors.initials: R
authors.lastname: Liu
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510640, PR China. Electronic address: cejqqu@scut.edu.cn.
authors.forename: Jinqing
authors.initials: J
authors.lastname: Qu
mesh_terms: D003952:Diagnostic Imaging / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration
chemical_list: D005456:Fluorescent Dyes
keywords: Bioimaging; Chemosensor; Fluorescent pH sensor; Styrylcyanine