title: Optimization of hydrolysis conditions for bovine plasma protein using response surface methodology.
abstract: The purpose of this study was to establish optimal conditions for the hydrolysis of bovine plasma protein. Response surface methodology was used to model and optimize responses [degree of hydrolysis (DH), 2,2-diphenyl-1-picrydrazyl (DPPH) radical-scavenging activity and Fe(2+)-chelating activity]. Hydrolysis conditions, such as hydrolysis temperature (46.6-63.4 °C), hydrolysis time (98-502 min), and hydrolysis pH (6.32-9.68) were selected as the main processing conditions in the hydrolysis of bovine plasma protein. Optimal conditions for maximum DH (%), DPPH radical-scavenging activity (%) and Fe(2+)-chelating activity (%) of the hydrolyzed bovine plasma protein, were respectively established. We discovered the following three conditions for optimal hydrolysis of bovine plasma: pH of 7.82-8.32, temperature of 54.1 °C, and time of 338.4-398.4 min. We consequently succeeded in hydrolyzing bovine plasma protein under these conditions and confirmed the various desirable properties of optimal hydrolysis.
authors.affiliation: Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Hyun-Woo
authors.initials: HW
authors.lastname: Seo
authors.affiliation: Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Jung
authors.affiliation: Department of Foods and Nutrition, Kookmin University, Seoul 136-702, Republic of Korea.
authors.forename: Gwang-Woong
authors.initials: GW
authors.lastname: Go
authors.affiliation: Department of Food Science and Biotechnology, Kyungnam University, Changwon 631-701, Republic of Korea.
authors.forename: Gap-Don
authors.initials: GD
authors.lastname: Kim
authors.affiliation: Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Seon-Tea
authors.initials: ST
authors.lastname: Joo
authors.affiliation: Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea. Electronic address: hsyang@gnu.ac.kr.
authors.forename: Han-Sul
authors.initials: HS
authors.lastname: Yang
mesh_terms: D000818:Animals; D001713:Biphenyl Compounds / Q000737:chemistry; D001798:Blood Proteins / Q000737:chemistry*; D002417:Cattle; D002614:Chelating Agents / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D010851:Picrates / Q000737:chemistry; D013381:Subtilisins / Q000737:chemistry; D013696:Temperature
chemical_list: D001713:Biphenyl Compounds; D001798:Blood Proteins; D002614:Chelating Agents; D010851:Picrates; C004931:1,1-diphenyl-2-picrylhydrazyl; D013381:Subtilisins
keywords: DPPH radical scavenging activity; Fe(2+) chelating activity; Optimization; Plasma protein hydrolysates; Response surface methodology