title: Sundew adhesive: a naturally occurring hydrogel.
abstract: Bioadhesives have drawn increasing interest in recent years, owing to their eco-friendly, biocompatible and biodegradable nature. As a typical bioadhesive, sticky exudate observed on the stalked glands of sundew plants aids in the capture of insects and this viscoelastic adhesive has triggered extensive interests in revealing the implied adhesion mechanisms. Despite the significant progress that has been made, the structural traits of the sundew adhesive, especially the morphological characteristics in nanoscale, which may give rise to the viscous and elastic properties of this mucilage, remain unclear. Here, we show that the sundew adhesive is a naturally occurring hydrogel, consisting of nano-network architectures assembled with polysaccharides. The assembly process of the polysaccharides in this hydrogel is proposed to be driven by electrostatic interactions mediated with divalent cations. Negatively charged nanoparticles, with an average diameter of 231.9 ± 14.8 nm, are also obtained from this hydrogel and these nanoparticles are presumed to exert vital roles in the assembly of the nano-networks. Further characterization via atomic force microscopy indicates that the stretching deformation of the sundew adhesive is associated with the flexibility of its fibrous architectures. It is also observed that the adhesion strength of the sundew adhesive is susceptible to low temperatures. Both elasticity and adhesion strength of the sundew adhesive reduce in response to lowering the ambient temperature. The feasibility of applying sundew adhesive for tissue engineering is subsequently explored in this study. Results show that the fibrous scaffolds obtained from sundew adhesive are capable of increasing the adhesion of multiple types of cells, including fibroblast cells and smooth muscle cells, a property that results from the enhanced adsorption of serum proteins. In addition, in light of the weak cytotoxic activity exhibited by these scaffolds towards a variety of mammal cells, evidence is sufficient to propose that sundew adhesive is a promising nanomaterial worth further exploitation in the field of tissue engineering.
authors.affiliation: Department of Biomedical Engineering, College of Engineering, The Ohio State University, Columbus, OH 43210, USA Dorothy M. Davis Heart and Lung Research Institute, Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USA Interdisciplinary Biophysics Graduate Program, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Yujian
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Biomedical Engineering, College of Engineering, The Ohio State University, Columbus, OH 43210, USA Dorothy M. Davis Heart and Lung Research Institute, Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USA Interdisciplinary Biophysics Graduate Program, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Yongzhong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Biomedical Engineering, College of Engineering, The Ohio State University, Columbus, OH 43210, USA Dorothy M. Davis Heart and Lung Research Institute, Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USA Interdisciplinary Biophysics Graduate Program, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Leming
authors.initials: L
authors.lastname: Sun
authors.affiliation: Department of Chemistry & Biochemistry, College of Arts and Sciences, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Richa
authors.initials: R
authors.lastname: Agrawal
authors.affiliation: Department of Biomedical Engineering, College of Engineering, The Ohio State University, Columbus, OH 43210, USA Dorothy M. Davis Heart and Lung Research Institute, Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USA Interdisciplinary Biophysics Graduate Program, The Ohio State University, Columbus, OH 43210, USA zhang.4882@osu.edu.
authors.forename: Mingjun
authors.initials: M
authors.lastname: Zhang
mesh_terms: D000269:Adhesives / Q000737:chemistry*; D000818:Animals; D002448:Cell Adhesion; D029787:Drosera / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D041681:NIH 3T3 Cells; D011134:Polysaccharides / Q000737:chemistry*
chemical_list: D000269:Adhesives; D020100:Hydrogels; D011134:Polysaccharides
keywords: nano-network; naturally occurring hydrogel; sundew adhesive; tissue engineering