title: Genetic diversity and differentiation in Prunus species (Rosaceae) using chloroplast and mitochondrial DNA CAPS markers.
abstract: Chloroplast (cpDNA) and mitochondrial DNA (mtDNA) were analyzed to establish genetic relationships among Tunisian plum cultivars using the polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) technique. Two mtDNA regions (nad 1 b/c and nad 4 1/2) and a cpDNA region (trnL-trnF) were amplified and digested using restriction enzymes. Seventy and six polymorphic sites were revealed in cpDNA and mtDNA, respectively. As a consequence, cpDNA appears to be more polymorphic than mtDNA. The unweighted pair group method with arithmetic mean (UPGMA) dendrogram showed that accessions were distributed independently of their geographical origin, and introduced and local cultivars appear to be closely related. Both UPGMA and principal component analysis grouped Tunisian plum accessions into similar clusters. The analysis of the pooled sequences allowed the detection of 17 chlorotypes and 12 mitotypes. The unique haplotypes detected for cultivars are valuable for management and preservation of the plum local resources. From this study, PCR-RFLP analysis appears to be a useful approach to detect and identify cytoplasmic variation in plum trees. Our results also provide useful information for the management of genetic resources and to establish a program to improve the genetic resources available for plums.
authors.affiliation: Laboratoire de Génétique Moléculaire, Immunologie & Biotechnologie, Faculté des Sciences de Tunis, Université Tunis El Manar, Campus Universitaire, El Manar Tunis, Tunisie.
authors.forename: S
authors.initials: S
authors.lastname: Ben Mustapha
authors.affiliation: Laboratoire de Génétique Moléculaire, Immunologie & Biotechnologie, Faculté des Sciences de Tunis, Université Tunis El Manar, Campus Universitaire, El Manar Tunis, Tunisie.
authors.forename: H
authors.initials: H
authors.lastname: Ben Tamarzizt
authors.affiliation: Laboratoire de Génétique Moléculaire, Immunologie & Biotechnologie, Faculté des Sciences de Tunis, Université Tunis El Manar, Campus Universitaire, El Manar Tunis, Tunisie.
authors.forename: G
authors.initials: G
authors.lastname: Baraket
authors.affiliation: Laboratoire de Génétique Moléculaire, Immunologie & Biotechnologie, Faculté des Sciences de Tunis, Université Tunis El Manar, Campus Universitaire, El Manar Tunis, Tunisie.
authors.forename: D
authors.initials: D
authors.lastname: Abdallah
authors.affiliation: Laboratoire de Génétique Moléculaire, Immunologie & Biotechnologie, Faculté des Sciences de Tunis, Université Tunis El Manar, Campus Universitaire, El Manar Tunis, Tunisie Amel.SalhiHannachi@fsb.rnu.tn.
authors.forename: A
authors.initials: A
authors.lastname: Salhi Hannachi
mesh_terms: D001483:Base Sequence; D002736:Chloroplasts / Q000235:genetics; D018742:DNA, Chloroplast / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics*; D005828:Genetics, Population; D018745:Genome, Plant; D005843:Geography; D006239:Haplotypes / Q000235:genetics; D008928:Mitochondria / Q000235:genetics; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D025341:Principal Component Analysis; D027861:Prunus / Q000145:classification / Q000235:genetics*
chemical_list: D018742:DNA, Chloroplast; D004272:DNA, Mitochondrial; D018744:DNA, Plant; D005819:Genetic Markers