title: Isolation and Seroprevalence of Aeromonas spp. Among Common Food Animals Slaughtered in Nagpur, Central India.
abstract: Aeromonads are ubiquitous foodborne pathogens with a global distribution. Animal-origin foods and contaminated animals are the main sources of Aeromonas infection to humans. So far little is known about the occurrence of Aeromonas spp. in food-producing animals in India. The present study was conducted to determine the prevalence and seroprevalence of Aeromonas species from 50 each of meat, blood, and sera samples collected from cattle, buffaloes, goats, and pigs slaughtered in and around Nagpur, Central India. Alkaline peptone water and ampicillin dextrin agar were used to isolate Aeromonas spp. An indirect enzyme-linked immunosorbent assay (ELISA) was standardized by use of whole-cell antigen (WC) and outer membrane protein (OMP) of Aeromonas hydrophila (MTCC 646). Aeromonads were isolated from 44 (22%) of the meat samples, and 1 (0.5%) from the blood samples. Seroprevalence by indirect ELISA-based WC antigen was estimated as 68% in cattle, 44% in buffaloes, 60% in goats, and 30% in pigs. OMP-based ELISA yielded a seroprevalence of 56%, 48%, 52%, and 22% in cattle, buffaloes, goats, and pigs, respectively. The results revealed that OMP-based ELISA and WC-based ELISA were in agreement with one another. Isolation along with high seropositivity demonstrates the presence of foodborne Aeromonas spp. in the Nagpur city of Central India.
authors.affiliation: 1 Department of Veterinary Public Health, Nagpur Veterinary College, Maharashtra Animal and Fishery Science University , Seminary Hills, Nagpur (Maharashtra), India .
authors.forename: Tanuja K G M
authors.initials: TK
authors.lastname: Gowda
authors.affiliation: 2 Department of Biochemistry, Indian Veterinary Research Institute , Bareilly, Uttar Pradesh, India .
authors.forename: Vishwanatha R A P
authors.initials: VR
authors.lastname: Reddy
authors.affiliation: 3 Department of Virology, Parasitology, and Immunology, Faculty of Veterinary Medicine, Ghent University , Merelbeke, Belgium .|4 Institute of Health and Society (IRSS), Faculty of Public Health, Université Catholique de Louvain , Brussels, Belgium .
authors.forename: Brecht
authors.initials: B
authors.lastname: Devleesschauwer
authors.affiliation: 1 Department of Veterinary Public Health, Nagpur Veterinary College, Maharashtra Animal and Fishery Science University , Seminary Hills, Nagpur (Maharashtra), India .
authors.forename: Nandkishor N
authors.initials: NN
authors.lastname: Zade
authors.affiliation: 1 Department of Veterinary Public Health, Nagpur Veterinary College, Maharashtra Animal and Fishery Science University , Seminary Hills, Nagpur (Maharashtra), India .
authors.forename: Sandeep P
authors.initials: SP
authors.lastname: Chaudhari
authors.affiliation: 1 Department of Veterinary Public Health, Nagpur Veterinary College, Maharashtra Animal and Fishery Science University , Seminary Hills, Nagpur (Maharashtra), India .
authors.forename: Waqar A
authors.initials: WA
authors.lastname: Khan
authors.affiliation: 1 Department of Veterinary Public Health, Nagpur Veterinary College, Maharashtra Animal and Fishery Science University , Seminary Hills, Nagpur (Maharashtra), India .
authors.forename: Shilpa V
authors.initials: SV
authors.lastname: Shinde
authors.affiliation: 1 Department of Veterinary Public Health, Nagpur Veterinary College, Maharashtra Animal and Fishery Science University , Seminary Hills, Nagpur (Maharashtra), India .
authors.forename: Archana R
authors.initials: AR
authors.lastname: Patil
mesh_terms: D000003:Abattoirs; D000333:Aeromonas / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism; D002020:Buffaloes; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D006041:Goats; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis / Q000662:veterinary; D007194:India; D008460:Meat / Q000382:microbiology*; D013552:Swine
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins