title: A cationic surfactant-decorated liquid crystal sensing platform for simple and sensitive detection of acetylcholinesterase and its inhibitor.
abstract: In this paper, construction of the liquid crystal (LC)-based sensing platform for simple and sensitive detection of acetylcholinesterase (AChE) and its inhibitor using a cationic surfactant-decorated LC interface was demonstrated. A change of the optical images of LCs from bright to dark appearance was observed when the cationic surfactant, myristoylcholine chloride (Myr), was transferred onto the aqueous/LC interface, due to the formation of a stable surfactant monolayer at the interface. A dark-to-bright change of the optical appearance was then observed when AChE was transferred onto the Myr-decorated LC interface. The sensitivity of this new type of LC-based sensor is 3 orders of magnitude higher in the serum albumin solution than that only in the buffer solution. Noteworthy is that the AChE LC sensor shows a very high sensitivity for the detection of the enzyme inhibitor, which is around 1 fM. The constructed low-cost LC-based sensor is quite simple and convenient, showing high promise for label-free detection of AChE and its inhibitors.
authors.affiliation: Key Laboratory of Colloid and Interface Chemistry, Shandong University, Ministry of Education, Jinan 250100, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Chemistry, University of Houston, Houston, TX 77204, United States.
authors.forename: Qiongzheng
authors.initials: Q
authors.lastname: Hu
authors.affiliation: Key Laboratory of Colloid and Interface Chemistry, Shandong University, Ministry of Education, Jinan 250100, PR China.
authors.forename: Yongxian
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Key Laboratory of Colloid and Interface Chemistry, Shandong University, Ministry of Education, Jinan 250100, PR China. Electronic address: ylmlt@sdu.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Yu
mesh_terms: D000110:Acetylcholinesterase / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D002794:Choline / Q000031:analogs & derivatives; D002800:Cholinesterase Inhibitors / Q000032:analysis* / Q000494:pharmacology; D057075:Enzyme Assays / Q000379:methods; D057230:Limit of Detection; D050866:Liquid Crystals / Q000737:chemistry*; D019814:Myristic Acid / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D002800:Cholinesterase Inhibitors; D013501:Surface-Active Agents; D019814:Myristic Acid; D000110:Acetylcholinesterase; D002794:Choline
keywords: Acetylcholinesterase; Cationic surfactant; Inhibitor; Liquid crystals; Myristoylcholine; Sensor