title: Brain-derived neurotrophic factor is a novel target gene of the has-miR-183/96/182 cluster in retinal pigment epithelial cells following visible light exposure.
abstract: Light-induced retinal injury is clinically and experimentally well-documented. It may be categorized into three types: Photothermal, photomechanical and photochemical injuries. To date, the variation in the hsa-miR-183/96/182 cluster and its potential target genes in human primary retinal pigment epithelial (RPE) cells, following visible light exposure, has not been reported. In the present study, RPE cells were exposed to 4 h of constant visible light. The expression of the hsa-miR-183/96/182 cluster was determined using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and its potential target genes were investigated. Additionally, hsa-miR-183, hsa-miR-96, hsa-miR-182 and has-miR-183/96/182 mimics were designed and synthesized in vitro, and transfected into the RPE cells. Subsequently, the expression of brain-derived neurotrophic factor (BDNF) mRNA and protein was measured, using RT-qPCR and western blotting, respectively. The regulation of miRNAs to the BDNF gene were then validated using a dual luciferase reporter gene assay system. The expression of hsa-miR-183, hsa-miR-96 and hsa-miR-182 significantly increased in RPE cells following 4 h of visible light exposure, compared with RPE cells that had been exposed to dark conditions (P<0.01). Following RPE cell transfection with mimics, BDNF mRNA and protein expression in the RPE cells was significantly downregulated compared with control RPE cells (P<0.05, P<0.01, respectively). Similarly, the ratio of Renilla luciferase/firefly luciferase significantly decreased in the RPE cells of the mimic + wild type (WT) group compared with cells of the psiCHECK(TM)-2 (a vector lacking the sequence of the BDNF gene), wild type and mimic + mutation groups (P<0.05, P<0.01). The present study suggests that BDNF is a target gene of the has-miR-183-96-182 cluster in RPE cells. The present study suggests an underlying protective mechanism against retinal light injury and may provide a novel target for the prevention and treatment of light-induced retinal injury.
authors.affiliation: Department of Opthalmology, The Chinese People's Liberation Army General Hospital, Beijing 100000, P.R. China.
authors.forename: Hongyang
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Opthalmology, The Chinese People's Liberation Army General Hospital, Beijing 100000, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Gong
authors.affiliation: Department of Opthalmology, The Chinese People's Liberation Army General Hospital, Beijing 100000, P.R. China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Qian
authors.affiliation: Department of Opthalmology, The Chinese People's Liberation Army General Hospital, Beijing 100000, P.R. China.
authors.forename: Tingjun
authors.initials: T
authors.lastname: Chen
authors.affiliation: Department of Opthalmology, The Chinese People's Liberation Army General Hospital, Beijing 100000, P.R. China.
authors.forename: Zihao
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Opthalmology, The Chinese People's Liberation Army General Hospital, Beijing 100000, P.R. China.
authors.forename: Zhaocai
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Department of Opthalmology, The Chinese People's Liberation Army General Hospital, Beijing 100000, P.R. China.
authors.forename: Shihui
authors.initials: S
authors.lastname: Wei
mesh_terms: D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D015536:Down-Regulation; D006801:Humans; D008027:Light; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D005810:Multigene Family; D016376:Oligonucleotides, Antisense / Q000378:metabolism; D012333:RNA, Messenger; D060888:Real-Time Polymerase Chain Reaction; D055213:Retinal Pigment Epithelium / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*
chemical_list: D019208:Brain-Derived Neurotrophic Factor; C533449:MIRN183 microRNA, human; C541492:MIRN96 microRNA, human; D035683:MicroRNAs; C538984:Mirn182 microRNA, human; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; C579455:BDNF protein, human