title: Effect of advanced glycosylation end products on apoptosis in human adipose tissue-derived stem cells in vitro.
abstract: BACKGROUND
  Both apoptosis and caspase-3 activity in adipose tissue-derived stem cells play an important role in the therapeutic process of diabetes patients. The purpose of this study was to investigate the effect of advanced glycation end products-human serum albumin (AGE-HSA) on apoptosis in human adipose tissue-derived stem cells (ADSCs) and to characterize the signal transduction pathways activated by AGEs that are involved in apoptosis regulation.
  
  
  RESULTS
  AGE-HSA promoted apoptosis and caspase-3 activity in ADSCs. However, the effects of AGE-HSA were significantly attenuated by an inhibitor of p38 MAPK, but not by inhibitors of JNK MAPK or ERK MAPK. AGE-HSA also upregulated the expression of RAGE. Silencing of the RAGE gene inhibited AGE-HSA-induced apoptosis, and activation and expression of phosphorylated p38 MAPK.
  
  
  CONCLUSIONS
  These results suggest that AGE-HSA promote the apoptosis of ADSCs in vitro via a RAGE-dependent p38 MAPK pathway.
authors.affiliation: Department of Blood Transfusion, Shengjing Hospital of China Medical University, No. 36 Sanhao Street, Heping District, Shenyang, 110004 P. R. China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Orthopaedics, Shengjing Hospital of China Medical University, No. 36 Sanhao Street, Heping District, Shenyang, 110004 P. R. China ; Department of Orthopedics, Central Hospital of Shenyang Medical College, No.5 Nanqi Road, Tiexi District, Shenyang, 110024 P.R. China.
authors.forename: Hongqiu
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Stem Cells and Regenerative Medicine, Key Laboratory of Cell Biology, Ministry of Public Health and Key Laboratory of Medical Cell Biology, Ministry of Education, China Medical University, No. 92 Bei'er Road, Heping District, Shenyang, 110001 P. R. China.
authors.forename: Dianbao
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Stem Cells and Regenerative Medicine, Key Laboratory of Cell Biology, Ministry of Public Health and Key Laboratory of Medical Cell Biology, Ministry of Education, China Medical University, No. 92 Bei'er Road, Heping District, Shenyang, 110001 P. R. China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Stem Cells and Regenerative Medicine, Key Laboratory of Cell Biology, Ministry of Public Health and Key Laboratory of Medical Cell Biology, Ministry of Education, China Medical University, No. 92 Bei'er Road, Heping District, Shenyang, 110001 P. R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhao
authors.affiliation: Department of Stem Cells and Regenerative Medicine, Key Laboratory of Cell Biology, Ministry of Public Health and Key Laboratory of Medical Cell Biology, Ministry of Education, China Medical University, No. 92 Bei'er Road, Heping District, Shenyang, 110001 P. R. China.
authors.forename: Xining
authors.initials: X
authors.lastname: Pang
authors.affiliation: Department of Blood Transfusion, Shengjing Hospital of China Medical University, No. 36 Sanhao Street, Heping District, Shenyang, 110004 P. R. China.
authors.forename: Qiushi
authors.initials: Q
authors.lastname: Wang
keywords: Advanced glycation end products; Apoptosis; Human adipose tissue-derived stem cells; Mitogen-activated protein kinase; Receptor for advanced glycation end products