title: Role of Chlamydia pneumoniae and Helicobacteria pylori in the development of tympanosclerosis.
abstract: The etiology of tympanosclerosis (TS) is not known, but TS commonly develops secondary to acute and chronic otitis media (COM). Since calcification process in TS resembles that of atherosclerosis (AS), pathogens that are related to pathogenesis of AS may be involved in development of TS. This prospective and controlled study, performed at a tertiary referral center, investigated a possible relationship between the presence of Chlamydia (C.) pneumoniae and Helicobacter (H.) pylori and the development of a tympanosclerotic plaque. The presence of C. pneumoniae was examined in the surgical specimens of 62 patients (29 females and 33 males; age range 10-70 years, mean age 30.8 ± 13.3 years), including 30 patients with TS, 14 patients with cholesteatoma, and 18 patients with chronic suppurative otitis media (CSOM). The presence of H. pylori was examined in the surgical specimens of 88 patients (41 females and 47 males; age range 6-70 years, mean age 32.5 ± 14.8 years), including 35 patients with TS, 22 patients with cholesteatoma, 20 patients with CSOM, and 11 patients with otosclerosis. Tympanosclerotic plaques and control specimens from the cholesteatoma, polypoid mucosa, or mucosal portion of the perforations and stapes supra structure were examined for the presence of H. pylori and/or C. pneumoniae using real-time polymerase chain reaction analysis. The analysis demonstrated that specimens from the tympanosclerotic plaques and the other types of COM were all negative for C. pneumoniae and H. pylori. An association between C. pneumoniae or H. pylori infection and the development of TS or other types of COM could not be established.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Faculty of Medicine, Bülent Ecevit University Hospital, Bülent Ecevit University, Kozlu, 67600, Zonguldak, Turkey. aykuterdem@yahoo.com.
authors.forename: Aykut Erdem
authors.initials: AE
authors.lastname: Dinç
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, Bülent Ecevit University Hospital, Bülent Ecevit University, Zonguldak, Turkey.
authors.forename: Füsun
authors.initials: F
authors.lastname: Cömert
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Faculty of Medicine, Bülent Ecevit University Hospital, Bülent Ecevit University, Kozlu, 67600, Zonguldak, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Damar
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Faculty of Medicine, Bülent Ecevit University Hospital, Bülent Ecevit University, Kozlu, 67600, Zonguldak, Turkey.
authors.forename: Sultan
authors.initials: S
authors.lastname: Şevik Eliçora
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Faculty of Medicine, Bülent Ecevit University Hospital, Bülent Ecevit University, Kozlu, 67600, Zonguldak, Turkey.
authors.forename: Duygu
authors.initials: D
authors.lastname: Erdem
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Faculty of Medicine, Bülent Ecevit University Hospital, Bülent Ecevit University, Kozlu, 67600, Zonguldak, Turkey.
authors.forename: Hüseyin
authors.initials: H
authors.lastname: Işık
mesh_terms: D000293:Adolescent; D000328:Adult; D016993:Chlamydophila pneumoniae / Q000302:isolation & purification*; D002908:Chronic Disease; D005260:Female; D016480:Helicobacter pylori / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D063371:Myringosclerosis / Q000209:etiology / Q000382:microbiology / Q000473:pathology; D010033:Otitis Media / Q000150:complications / Q000175:diagnosis / Q000503:physiopathology; D011446:Prospective Studies; D013223:Statistics as Topic; D014421:Turkey; D014432:Tympanic Membrane / Q000382:microbiology / Q000473:pathology
keywords: Chlamydia pneumoniae; Chronic otitis media; Helicobacter pylori; Tympanosclerosis