title: Solution NMR assignment of the cryptic sixth TOG domain of mini spindles.
abstract: TOG domains contribute to the organisation of microtubules through their ability to bind tubulin. They are found in members of the XMAP215 family of proteins, which act as microtubule polymerases and fulfill important roles in the formation of the mitotic spindle and in the assembly of kinetochore fibres. We recently identified a cryptic TOG domain in the XMAP215 family proteins, chTOG and its Drosophila homologue, mini spindles. This domain is not part of the well-established array of TOG domains involved in tubulin polymerisation. Instead it forms part of a binding site for TACC3 family proteins. This interaction is required for the assembly of kinetochore bridges in a trimeric complex with clathrin. Here we present the first NMR assignment of a sixth TOG domain from mini spindles as a first step to elucidate its structure and function.
authors.affiliation: Department of Biochemistry, University of Leicester, Lancaster Road, Leicester, LE1 9HN, UK.|Cancer Research UK Leicester Centre, Lancaster Road, Leicester, LE1 9HN, UK.
authors.forename: Selena G
authors.initials: SG
authors.lastname: Burgess
authors.affiliation: Department of Biochemistry, University of Leicester, Lancaster Road, Leicester, LE1 9HN, UK.|Cancer Research UK Leicester Centre, Lancaster Road, Leicester, LE1 9HN, UK.
authors.forename: Richard
authors.initials: R
authors.lastname: Bayliss
authors.affiliation: Cardiovascular and Randall Division, King's College London, Guy's Campus, London, SE1 1UL, UK. mark.pfuhl@kcl.ac.uk.
authors.forename: Mark
authors.initials: M
authors.lastname: Pfuhl
mesh_terms: D000818:Animals; D029721:Drosophila Proteins / Q000737:chemistry*; D004331:Drosophila melanogaster / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D017434:Protein Structure, Tertiary; D066244:Proton Magnetic Resonance Spectroscopy; D012996:Solutions
chemical_list: D029721:Drosophila Proteins; D008869:Microtubule-Associated Proteins; D012996:Solutions; C121511:msps protein, Drosophila
keywords: HEAT repeats; Kinetochore; Mitosis; TACC3; chTOG