title: Relationship between serum GAD-Ab and the genetic polymorphisms of GAD2 and type 2 diabetes mellitus.
abstract: In this study, we investigated the relationship between serum glutamic acid decarboxylase (GAD) autoantibody (Ab) levels and single nucleotide polymorphisms (SNPs) of the glutamic acid de-carboxylase 2 (GAD2) 5'-untranslated region and the susceptibility to type 2 diabetes in the Han population. The distributions of patients with SNPs in the GAD2 5'-untranslated region (rs2236418, rs185649317, and rs8190590) and type 2 diabetes and that of the healthy group were genotyped and analyzed using Sequenom MassArray SNP genotyp-ing. GAD-Ab levels were also detected. The frequency distributions of the AA, AG, and GG genotypes in the polymorphic site rs2236418 in the diabetes GAD-Ab-positive group were 45.9, 42.8, and 11.4%, respectively, whereas those in the control group were 36.6, 43.7, and 19.8%, respectively. The difference between the 2 groups was statis-tically significant (P < 0.05). Unlike the GG genotype, the AA and AA + AG genotypes increased the risk of GAD-Ab (odds ratios (95% confidence intervals) = 2.623 (1.351-4.937) and 2.152 (1.375-4.202), respectively). The associations of the 3 SNPs of the GAD2 gene 5'-un-translated region polymorphisms with susceptibility to type 2 diabe-tes in the Chongqing Han population were significant. The SNP of rs2236418 in the Chongqing Han population of diabetic patients with serum GAD-Ab levels was significantly correlated with the SNPs rs185649317 and rs8190590.
authors.affiliation: Department of Laboratory Medicine, People's Hospital of Changshou, Chongqing, China qlzrcn@126.com.
authors.forename: Q
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Endocrinology, People's Hospital of Changshou, Chongqing, China.
authors.forename: Z R
authors.initials: ZR
authors.lastname: Qiao
authors.affiliation: Department of Laboratory Medicine, People's Hospital of Changshou, Chongqing, China.
authors.forename: D B
authors.initials: DB
authors.lastname: Liu
authors.affiliation: Department of Laboratory Medicine, People's Hospital of Changshou, Chongqing, China.
authors.forename: J T
authors.initials: JT
authors.lastname: Zeng
authors.affiliation: Department of Laboratory Medicine, People's Hospital of Changshou, Chongqing, China.
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Laboratory Medicine, People's Hospital of Changshou, Chongqing, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Bo
authors.affiliation: Department of Laboratory Medicine, People's Hospital of Changshou, Chongqing, China.
authors.forename: H Y
authors.initials: HY
authors.lastname: Zu
authors.affiliation: Department of Endocrinology, People's Hospital of Changshou, Chongqing, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Hu
authors.affiliation: Department of Endocrinology, People's Hospital of Changshou, Chongqing, China.
authors.forename: X
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Laboratory Medicine, People's Hospital of Changshou, Chongqing, China.
authors.forename: S S
authors.initials: SS
authors.lastname: Dong
mesh_terms: D000368:Aged; D001323:Autoantibodies / Q000097:blood* / Q000235:genetics / Q000276:immunology; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000201:enzymology* / Q000235:genetics / Q000276:immunology*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005968:Glutamate Decarboxylase / Q000097:blood / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide
chemical_list: D001323:Autoantibodies; D005968:Glutamate Decarboxylase; C401141:glutamate decarboxylase 2