title: A hybrid-membrane migration method to isolate high-purity adipose-derived stem cells from fat tissues.
abstract: Human adipose-derived stem cells (hADSCs) exhibit heterogeneous characteristics, indicating various genotypes and differentiation abilities. The isolated hADSCs can possess different purity levels and divergent properties depending on the purification methods used. We developed a hybrid-membrane migration method that purifies hADSCs from a fat tissue solution with extremely high purity and pluripotency. A primary fat-tissue solution was permeated through the porous membranes with a pore size from 8 to 25 μm, and the membranes were incubated in cell culture medium for 15-18 days. The hADSCs that migrated from the membranes contained an extremely high percentage (e.g., >98%) of cells positive for mesenchymal stem cell markers and showed almost one order of magnitude higher expression of some pluripotency genes (Oct4, Sox2, Klf4 and Nanog) compared with cells isolated using the conventional culture method.
authors.affiliation: 1] Department of Chemical and Materials Engineering, National Central University, Jhong-li, Taoyuan, Taiwan [2] Department of Botany and Microbiology, King Saud University, Riyadh, Saudi Arabia [3] Department of Reproduction, National Research Institute for Child Health and Development, Tokyo, Japan [4] Nano Medical Engineering Laboratory, RIKEN, Wako, Saitama, Japan.
authors.forename: Akon
authors.initials: A
authors.lastname: Higuchi
authors.affiliation: Department of Chemical and Materials Engineering, National Central University, Jhong-li, Taoyuan, Taiwan.
authors.forename: Ching-Tang
authors.initials: CT
authors.lastname: Wang
authors.affiliation: 1] Institute of Systems Biology and Bioinformatics, National Central University, Jhong-li, Taoyuan, Taiwan [2] Cathay Medical Research Institute, Cathay General Hospital, Taipei, Taiwan.
authors.forename: Qing-Dong
authors.initials: QD
authors.lastname: Ling
authors.affiliation: Department of Surgery, Cathay General Hospital, Taipei, Taiwan.
authors.forename: Henry Hsin-chung
authors.initials: HH
authors.lastname: Lee
authors.affiliation: Department of Medical Microbiology and Parasitology, Universities Putra Malaysia, Slangor, Malaysia.
authors.forename: S Suresh
authors.initials: SS
authors.lastname: Kumar
authors.affiliation: Department of Chemical Engineering, R&D Center for Membrane Technology, Chung Yuan Christian University, Jhong-li, Taoyuan, Taiwan.
authors.forename: Yung
authors.initials: Y
authors.lastname: Chang
authors.affiliation: Department of Botany and Microbiology, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Abdullah A
authors.initials: AA
authors.lastname: Alarfaj
authors.affiliation: Department of Botany and Microbiology, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Murugan A
authors.initials: MA
authors.lastname: Munusamy
authors.affiliation: Department of Internal Medicine, Taiwan Landseed Hospital, Pingjen, Taoyuan, Taiwan.
authors.forename: Shih-Tien
authors.initials: ST
authors.lastname: Hsu
authors.affiliation: Graduate Institute of Medical Sciences and Department of Obstetrics &Gynecology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan.
authors.forename: Gwo-Jang
authors.initials: GJ
authors.lastname: Wu
authors.affiliation: Department of Reproduction, National Research Institute for Child Health and Development, Tokyo, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Umezawa
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D053687:Adult Stem Cells / Q000166:cytology* / Q000378:metabolism; D000954:Antigens, Surface / Q000378:metabolism; D015415:Biomarkers; D002469:Cell Separation / Q000379:methods*; D020869:Gene Expression Profiling; D006801:Humans; D016130:Immunophenotyping; D000090062:Kruppel-Like Factor 4
chemical_list: D000954:Antigens, Surface; D015415:Biomarkers; C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4