title: Markers for the population genetics studies of Triatoma sordida (Hemiptera: Reduviidae).
abstract: BACKGROUND
  Triatoma sordida, a vector of Trypanosoma cruzi, is native of Brazil, Bolivia, Paraguay, Argentina, and Uruguay, and occurs primarily in peridomiciles. Currently, it is the species most frequently captured by the Chagas Disease Control Program in Brazil. For this reason, population genetic studies attract great interest, as they can provide further information about the dispersal and household invasion processes of this species. In the absence of suitable markers, the objective of this study was to test the cross amplification of microsatellite primers.
  
  
  RESULTS
  23 primers were tested for microsatellite loci already described for other species of the genus Triatoma sp. Forty four specimens of T. sordida captured in the north of Minas Gerais were used to validate the use of standardized loci for population genetic analyses. It was possible to amplify 10 of the 23 loci tested for T. sordida.
  
  
  CONCLUSIONS
  This is the first study that provides 10 microsatellite markers for population analysis of this triatomine species. Cross-amplification of primers can be used among other phylogenetically related species whose loci are already available for study.
authors.affiliation: Laboratório de Triatomíneos e Epidemiologia da Doença de Chagas, Centro de Pesquisas René Rachou, Avenida Augusto de Lima, 1715 Barro Preto, CEP 30.190-002, Belo Horizonte, Minas Gerais, Brazil. carlotabelisario@yahoo.com.br.
authors.forename: Carlota Josefovicz
authors.initials: CJ
authors.lastname: Belisário
authors.affiliation: Laboratório de Triatomíneos e Epidemiologia da Doença de Chagas, Centro de Pesquisas René Rachou, Avenida Augusto de Lima, 1715 Barro Preto, CEP 30.190-002, Belo Horizonte, Minas Gerais, Brazil. grasiellepessoa@hotmail.com.
authors.forename: Grasielle Caldas D'Ávila
authors.initials: GC
authors.lastname: Pessoa
authors.affiliation: Laboratório de Triatomíneos e Epidemiologia da Doença de Chagas, Centro de Pesquisas René Rachou, Avenida Augusto de Lima, 1715 Barro Preto, CEP 30.190-002, Belo Horizonte, Minas Gerais, Brazil. fernandes.santos.paula@gmail.com.
authors.forename: Paula Fernandes
authors.initials: PF
authors.lastname: dos Santos
authors.affiliation: Laboratório de Triatomíneos e Epidemiologia da Doença de Chagas, Centro de Pesquisas René Rachou, Avenida Augusto de Lima, 1715 Barro Preto, CEP 30.190-002, Belo Horizonte, Minas Gerais, Brazil. leticia.sena@gmail.com.
authors.forename: Letícia Sena
authors.initials: LS
authors.lastname: Dias
authors.affiliation: Laboratório de Triatomíneos e Epidemiologia da Doença de Chagas, Centro de Pesquisas René Rachou, Avenida Augusto de Lima, 1715 Barro Preto, CEP 30.190-002, Belo Horizonte, Minas Gerais, Brazil. aline.rosa@cpqrr.fiocruz.br.
authors.forename: Aline Cristine Luiz
authors.initials: AC
authors.lastname: Rosa
authors.affiliation: Laboratório de Triatomíneos e Epidemiologia da Doença de Chagas, Centro de Pesquisas René Rachou, Avenida Augusto de Lima, 1715 Barro Preto, CEP 30.190-002, Belo Horizonte, Minas Gerais, Brazil. diotaiuti@cpqrr.fiocruz.br.
authors.forename: Liléia
authors.initials: L
authors.lastname: Diotaiuti
mesh_terms: D000818:Animals; D014355:Chagas Disease / Q000635:transmission*; D017931:DNA Primers / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D005828:Genetics, Population; D007303:Insect Vectors / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D013020:South America; D014224:Triatoma / Q000235:genetics*; D014349:Trypanosoma cruzi / Q000502:physiology*
chemical_list: D017931:DNA Primers; D005819:Genetic Markers