title: TopKLists: a comprehensive R package for statistical inference, stochastic aggregation, and visualization of multiple omics ranked lists.
abstract: High-throughput sequencing techniques are increasingly affordable and produce massive amounts of data. Together with other high-throughput technologies, such as microarrays, there are an enormous amount of resources in databases. The collection of these valuable data has been routine for more than a decade. Despite different technologies, many experiments share the same goal. For instance, the aims of RNA-seq studies often coincide with those of differential gene expression experiments based on microarrays. As such, it would be logical to utilize all available data. However, there is a lack of biostatistical tools for the integration of results obtained from different technologies. Although diverse technological platforms produce different raw data, one commonality for experiments with the same goal is that all the outcomes can be transformed into a platform-independent data format - rankings - for the same set of items. Here we present the R package TopKLists, which allows for statistical inference on the lengths of informative (top-k) partial lists, for stochastic aggregation of full or partial lists, and for graphical exploration of the input and consolidated output. A graphical user interface has also been implemented for providing access to the underlying algorithms. To illustrate the applicability and usefulness of the package, we integrated microRNA data of non-small cell lung cancer across different measurement techniques and draw conclusions. The package can be obtained from CRAN under a LGPL-3 license.
authors.forename: Michael G
authors.initials: MG
authors.lastname: Schimek
authors.forename: Eva
authors.initials: E
authors.lastname: Budinská
authors.forename: Karl G
authors.initials: KG
authors.lastname: Kugler
authors.forename: Vendula
authors.initials: V
authors.lastname: Švendová
authors.forename: Jie
authors.initials: J
authors.lastname: Ding
authors.forename: Shili
authors.initials: S
authors.lastname: Lin
mesh_terms: D019295:Computational Biology / Q000379:methods; D020869:Gene Expression Profiling / Q000379:methods; D023281:Genomics / Q000379:methods*; D035683:MicroRNAs / Q000235:genetics; D015233:Models, Statistical; D012984:Software
chemical_list: D035683:MicroRNAs