title: [Antiproliferative Effect of Specific Inhibitor XAV939 for β-catenin on MCL Jeko-1 Cells].
abstract: OBJECTIVE
  To investigate the effect of short hairpin RNA (shRNA) and XAV939, a specific inhibitor for β-catenin, on growth and apoptosis of mantle cell lymphoma(MCL) Jeko-1 cell line.
  
  
  METHODS
  β-catenin shRNA eukaryotic expression vector was transfected into Jeko-1 cells, the antiproliferative effect of shRNA on Jeko-1 cells was detected by RT-PCR and Western blot. The proliferation inhibitory rate of Jeko-1 cells treated by different doses of XAV939 was assayed by MTT method; the level of apoptosis of Jeko-1 cells was detected by flow cytometry; the expression levels of apoptosis-related protein BCL-2, BAX, CyclinD1, C-MYC and Caspase-3 in Jeko-1 cells were determined by Western blot.
  
  
  RESULTS
  The expression of β-catenin mRNA and growth of Jeko-1 cell line were inhibited by shRNA; after Jeko-1 cells treated with 0,2 and 8 µmol/L XAV939 for 48 hours, the cell proliferation rate decreased, while the cell apoptosis rate increased, the expressions of apoptosis-related protein BCL-2, CyclinD1 and C-MYC were down-regulated, on the contrary, the expression of BAX and caspase-3 were up-regulated.
  
  
  CONCLUSIONS
  The specific inhibition of β-catenin can inhibit Jeko-1 cell proliferation and promote the cell apoptosis.
authors.affiliation: Department of Pediatrics, Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou 363000, Fujian Province, China.
authors.forename: Jin-Shui
authors.initials: JS
authors.lastname: He
authors.affiliation: Department of Hematology, Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou 363000, Fujian Province, China.
authors.forename: Yi-Qun
authors.initials: YQ
authors.lastname: Huang
authors.affiliation: Department of Pathology, Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou 363000, Fujian Province, China.
authors.forename: Jian-Ming
authors.initials: JM
authors.lastname: Weng
authors.affiliation: Department of Pediatrics, Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou 363000, Fujian Province, China.
authors.forename: Li-Yun
authors.initials: LY
authors.lastname: Xiao
authors.affiliation: Department of Pediatrics, Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou 363000, Fujian Province, China.
authors.forename: Kai-Zhi
authors.initials: KZ
authors.lastname: Weng
authors.affiliation: Department of Hematology, Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou 363000, Fujian Province, China. E-mail: 353624974@qq.com.
authors.forename: Xu-Dong
authors.initials: XD
authors.lastname: Ma
mesh_terms: D017209:Apoptosis; D053148:Caspase 3; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006575:Heterocyclic Compounds, 3-Ring; D006801:Humans; D020522:Lymphoma, Mantle-Cell; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D014162:Transfection; D051176:beta Catenin
chemical_list: C495270:CTNNB1 protein, human; D006575:Heterocyclic Compounds, 3-Ring; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C544261:XAV939; D051176:beta Catenin; C505407:CASP3 protein, human; D053148:Caspase 3