title: Characterization of a Novel Mouse Model of Alzheimer's Disease--Amyloid Pathology and Unique β-Amyloid Oligomer Profile.
abstract: Amyloid plaques composed of β-amyloid (Aβ) protein are a pathological hallmark of Alzheimer's disease. We here report the generation and characterization of a novel transgenic mouse model of Aβ toxicity. The rTg9191 mice harbor a transgene encoding the 695 amino-acid isoform of human amyloid precursor protein (APP) with the Swedish and London mutations (APPNLI) linked to familial Alzheimer's disease, under the control of a tetracycline-response element, as well as a transgene encoding the tetracycline transactivator, under the control of the promoter for calcium-calmodulin kinase IIα. In these mice, APPNLI is expressed at a level four-fold that of endogenous mouse APP and its expression is restricted to forebrain regions. Transgene expression was suppressed by 87% after two months of doxycycline administration. Histologically, we showed that (1) Aβ plaques emerged in cerebral cortex and hippocampus as early as 8 and 10.5-12.5 months of age, respectively; (2) plaque deposition progressed in an age-dependent manner, occupying up to 19% of cortex at ~25 months of age; and (3) neuropathology--such as abnormal neuronal architecture, tau hyperphosphorylation and misfolding, and neuroinflammation--was observed in the vicinity of neuritic plaques. Biochemically, we determined total Aβ production at varied ages of mice, and we showed that mice produced primarily fibrillar Aβ assemblies recognized by conformation-selective OC antibodies, but few non-fibrillar oligomers (e.g., Aβ*56) detectable by A11 antibodies. Finally, we showed that expression of the tetracycline transactivator resulted in reduced brain weight and smaller dentate-gyrus size. Collectively, these data indicate that rTg9191 mice may serve as a model for studying the neurological effects of the fibrillar Aβ assemblies in situ.
authors.affiliation: Department of Neurology, University of Minnesota, Minneapolis, Minnesota, United States of America; N. Bud Grossman Center for Memory Research and Care, University of Minnesota, Minneapolis, Minnesota, United States of America.
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.affiliation: Department of Neurology, University of Minnesota, Minneapolis, Minnesota, United States of America; N. Bud Grossman Center for Memory Research and Care, University of Minnesota, Minneapolis, Minnesota, United States of America.
authors.forename: Jennifer B
authors.initials: JB
authors.lastname: Paulson
authors.affiliation: University of Minnesota Academic Health Center Biological Materials Procurement Network (BioNet), University of Minnesota, Minneapolis, Minnesota, United States of America; N. Bud Grossman Center for Memory Research and Care, University of Minnesota, Minneapolis, Minnesota, United States of America.
authors.forename: Colleen L
authors.initials: CL
authors.lastname: Forster
authors.affiliation: Department of Neurology, University of Minnesota, Minneapolis, Minnesota, United States of America; N. Bud Grossman Center for Memory Research and Care, University of Minnesota, Minneapolis, Minnesota, United States of America.
authors.forename: Samantha L
authors.initials: SL
authors.lastname: Shapiro
authors.affiliation: Department of Neurology, University of Minnesota, Minneapolis, Minnesota, United States of America; N. Bud Grossman Center for Memory Research and Care, University of Minnesota, Minneapolis, Minnesota, United States of America; Department of Neuroscience, University of Minnesota, Minneapolis, Minnesota, United States of America; Geriatric Research Education and Clinical Centers, Veterans Affairs Medical Center, Minneapolis, Minnesota, United States of America.
authors.forename: Karen H
authors.initials: KH
authors.lastname: Ashe
authors.affiliation: Department of Neurology, University of Minnesota, Minneapolis, Minnesota, United States of America; N. Bud Grossman Center for Memory Research and Care, University of Minnesota, Minneapolis, Minnesota, United States of America.
authors.forename: Kathleen R
authors.initials: KR
authors.lastname: Zahs
mesh_terms: D000544:Alzheimer Disease / Q000235:genetics / Q000473:pathology*; D016229:Amyloid beta-Peptides / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002540:Cerebral Cortex / Q000473:pathology; D004195:Disease Models, Animal; D004318:Doxycycline / Q000494:pharmacology; D005260:Female; D006624:Hippocampus / Q000473:pathology; D006801:Humans; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D058225:Plaque, Amyloid / Q000473:pathology*; D016548:Prosencephalon / Q000378:metabolism*; D013752:Tetracycline
chemical_list: D016229:Amyloid beta-Peptides; D013752:Tetracycline; D004318:Doxycycline