title: Genetic variants of the unsaturated fatty acid receptor GPR120 relating to obesity in dogs.
abstract: G protein-coupled receptor (GPR) 120 is an unsaturated fatty acid receptor, which is associated with various physiological functions. It is reported that the genetic variant of GPR120, p.Arg270His, is detected more in obese people, and this genetic variation functionally relates to obesity in humans. Obesity is a common nutritional disorder also in dogs, but the genetic factors have not ever been identified in dogs. In this study, we investigated the molecular structure of canine GPR120 and searched for candidate genetic variants which may relate to obesity in dogs. Canine GPR120 was highly homologous to those of other species, and seven transmembrane domains and two N-glycosylation sites were conserved. GPR120 mRNA was expressed in lung, jejunum, ileum, colon, hypothalamus, hippocampus, spinal cord, bone marrow, dermis and white adipose tissues in dogs, as those in mice and humans. Genetic variants of GPR120 were explored in client-owned 141 dogs, resulting in that 5 synonymous and 4 non-synonymous variants were found. The variant c.595C>A (p.Pro199Thr) was found in 40 dogs, and the gene frequency was significantly higher in dogs with higher body condition scores, i.e. 0.320 in BCS4-5 dogs, 0.175 in BCS3 dogs and 0.000 in BCS2 dogs. We conclude that c.595C>A (p.Pro199Thr) is a candidate variant relating to obesity, which may be helpful for nutritional management of dogs.
authors.affiliation: Department of Veterinary Nursing, School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino-shi, Tokyo 180-8602, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Miyabe
authors.forename: Azusa
authors.initials: A
authors.lastname: Gin
authors.forename: Eri
authors.initials: E
authors.lastname: Onozawa
authors.forename: Mana
authors.initials: M
authors.lastname: Daimon
authors.forename: Hana
authors.initials: H
authors.lastname: Yamada
authors.forename: Hitomi
authors.initials: H
authors.lastname: Oda
authors.forename: Akihiro
authors.initials: A
authors.lastname: Mori
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Momota
authors.forename: Daigo
authors.initials: D
authors.lastname: Azakami
authors.forename: Ichiro
authors.initials: I
authors.lastname: Yamamoto
authors.forename: Mariko
authors.initials: M
authors.lastname: Mochizuki
authors.forename: Toshinori
authors.initials: T
authors.lastname: Sako
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Tamura
authors.forename: Katsumi
authors.initials: K
authors.lastname: Ishioka
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000235:genetics*; D004285:Dogs; D005786:Gene Expression Regulation / Q000502:physiology*; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D009765:Obesity / Q000235:genetics / Q000662:veterinary*; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*
chemical_list: D043562:Receptors, G-Protein-Coupled