title: Elucidation of the mass fragmentation pathways of tomatidine and β1-hydroxytomatine using orbitrap mass spectrometry.
abstract: Tomatoes, members of the Solanaceae plant family, produce biologically active secondary metabolites, including glycoalkaloids, which may have both adverse and beneficial biological effects. Using the linear ion trap (LIT) mass spectrometry, multi-stage collision induced dissociation (CID) experiments (MSn) were performed to elucidate characteristic fragmentation pathways of the glycoalkaloid, tomatidine and of β1-hydroxytomatine. High resolution with high accuracy mass analysis using an Orbitrap fourier transform MS with higher-energy collisional induced dissociation (HCD) was used to produce mass spectra data across a wide spectral range for confirmation of proposed ion structures and formulae.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Caprioli
authors.forename: Michael
authors.initials: M
authors.lastname: Cahill
authors.forename: Serena
authors.initials: S
authors.lastname: Logrippo
authors.forename: Kevin
authors.initials: K
authors.lastname: James
mesh_terms: D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D014053:Tomatine / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: C000598617:beta1-hydroxytomatine; C003677:tomatidine; D014053:Tomatine