title: C-phycocyanin prevents cisplatin-induced mitochondrial dysfunction and oxidative stress.
abstract: The potential of C-phycocyanin (C-PC) to prevent cisplatin (CP)-induced kidney mitochondrial dysfunction was determined in CD-1 male mice. The CP-induced mitochondrial dysfunction was characterized by ultrastructural abnormalities and by decrease in the following parameters in isolated kidney mitochondria: adenosine diphosphate (ADP)-induced oxygen consumption (state 3), respiratory control ratio, ADP/oxygen (ADP/O) ratio, adenosine triphosphate synthesis, membrane potential, calcium retention, glutathione (GSH) content, and activity of respiratory complex I, aconitase, catalase, and GSH peroxidase. These mitochondria also showed increase in hydrogen peroxide production, malondialdehyde, and 3-nitrotyrosine protein adducts content. The above-described changes, as well as CP-induced nephrotoxicity, were attenuated in mice pretreated with a single injection of C-PC. Our data suggest that the attenuation of mitochondrial abnormalities is involved in the protective effect of C-PC against CP-induced nephrotoxicity. This is the first demonstration that C-PC pretreatment prevents CP-induced mitochondrial dysfunction in mice.
authors.affiliation: Department of Biology, Facultad de Química, UNAM, Ciudad Universitaria, 04510, Mexico, D.F., Mexico.
authors.forename: Berenice
authors.initials: B
authors.lastname: Fernández-Rojas
authors.forename: Daniela Sarai
authors.initials: DS
authors.lastname: Rodríguez-Rangel
authors.forename: Luis Fernando
authors.initials: LF
authors.lastname: Granados-Castro
authors.forename: Mario
authors.initials: M
authors.lastname: Negrette-Guzmán
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: León-Contreras
authors.forename: Rogelio
authors.initials: R
authors.lastname: Hernández-Pando
authors.forename: Eduardo
authors.initials: E
authors.lastname: Molina-Jijón
authors.forename: José L
authors.initials: JL
authors.lastname: Reyes
authors.forename: Cecilia
authors.initials: C
authors.lastname: Zazueta
authors.forename: José
authors.initials: J
authors.lastname: Pedraza-Chaverri
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D001806:Blood Urea Nitrogen; D002118:Calcium / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002945:Cisplatin / Q000633:toxicity*; D003404:Creatinine / Q000097:blood; D004353:Drug Evaluation, Preclinical; D004579:Electron Transport; D005979:Glutathione Peroxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D051379:Mice; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress; D010101:Oxygen Consumption; D010798:Phycocyanin / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D010798:Phycocyanin; D008315:Malondialdehyde; D000255:Adenosine Triphosphate; D003404:Creatinine; D006861:Hydrogen Peroxide; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D002945:Cisplatin; D002118:Calcium