title: Armillaria mellea: phenolic content, in vitro antioxidant and antihyperglycemic effects.
abstract: Armillaria mellea, known as honey mushroom, has been used both as food and medicine.
  
  
  OBJECTIVE
  In this work, the ethanolic and hydromethanolic extracts from Armillaria mellea fruiting bodies were investigated for their phenolic content, antioxidant and antihyperglycemic effects (in vitro studies).
  
  
  METHODS
  The total phenolics were quantified using Folin-Ciocalteu assay. The antioxidant activity was evaluated by testing the free radical scavenging capacity, reducing power and ferrous ion chelating ability; in addition, the capacity to inhibit 15-lipoxygenase was also assessed. The antihyperglycemic activity was investigated by α-glucosidase assay.
  
  
  RESULTS
  Total phenolic contents of 21.68 ± 0.06 and 5.70 ± 0.28 mg/g were determined in the hydromethanolic and ethanolic extracts, respectively. The hydromethanolic extract showed higher free radical scavenging and reducing abilities (EC50 = 452.6 ± 2.7, 140.57 ± 1.45 and 129.45 ± 0.98 μg/mL in DPPH, ABTS and reducing power assays, respectively). The ethanolic extract proved to be more efficient in the ferrous ion chelation, 15-lipoxygenase and α-glucosidase inhibition assays (EC55 = 67.93 ± 0.35, 290.93 ± 2.05 and 8.54 ± 0.06 μg/mL, respectively).
  
  
  CONCLUSIONS
  Armillaria mellea extracts showed antioxidant and antihyperglycemic potential in in vitro models and therefore they are promising candidates for the development of dietary supplements and pharmaceutical products.
authors.forename: Daniela Elena
authors.initials: DE
authors.lastname: Zavastin
authors.forename: Cornelia
authors.initials: C
authors.lastname: Mircea
authors.forename: Ana Clara
authors.initials: AC
authors.lastname: Aprotosoaie
authors.forename: Simona
authors.initials: S
authors.lastname: Gherman
authors.forename: Monica
authors.initials: M
authors.lastname: Hancianu
authors.forename: Anca
authors.initials: A
authors.lastname: Miron
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D055392:Armillaria / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry* / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000737:chemistry; D007004:Hypoglycemic Agents / Q000737:chemistry* / Q000494:pharmacology; D066298:In Vitro Techniques / Q000379:methods; D010636:Phenols / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D016166:Free Radical Scavengers; D007004:Hypoglycemic Agents; D010636:Phenols