title: Assessing the Chemical Composition and Antimicrobial Activity of Essential Oils from Brazilian Plants-Eremanthus erythropappus (Asteraceae), Plectrantuns barbatus, and P. amboinicus (Lamiaceae).
abstract: The chemical composition and antimicrobial activity of essential oils obtained from three Brazilian plant species-leaves and branches of Eremanthus erythropappus (Asteraceae), leaves of Plectranthus barbatus, and leaves of P. amboinicus (Lamiaceae)-were determined. Analysis by GC/MS and determination of Kovats indexes both indicated δ-elemene (leaves-42.61% and branches-23.41%) as well as (-)-α-bisabolol (leaves-24.80% and stem bark-66.16%) as major constituents of E. erythropappus essential oils. The main components of leaves of P. barbatus were identified as (Z)-caryophyllene (17.98%), germacrene D (17.35%), and viridiflorol (14.13%); whereas those of leaves of P. amboinicus were characterized as p-cymene (12.01%), γ-terpinene (14.74%), carvacrol (37.70%), and (Z)-caryophyllene (14.07%). The antimicrobial activity against yeasts and bacteria was assessed in broth microdilution assays to determine the minimum inhibitory concentration (MIC) necessary to inhibit microbial growth. In addition, the crude oil of branches of E. erythropappus was subjected to chromatographic separation procedures to afford purified (-)-α-bisabolol. This compound displayed biological activity against pathogenic yeasts, thus suggesting that the antimicrobial effect observed with crude oils of E. erythropappus leaves and branches may be related to the occurrence of (-)-α-bisabolol as their main component. Our results showed that crude oils of Brazilian plants, specifically E. erythropappus, P. barbatus, and P. amboinicus and its components, could be used as a tool for the developing novel and more efficacious antimicrobial agents.
authors.affiliation: Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, 09972-270 Diadema, SP, Brazil. nara.oshiro@gmail.com.
authors.forename: Nara O Dos
authors.initials: NO
authors.lastname: Santos
authors.affiliation: Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, 09972-270 Diadema, SP, Brazil. bruna_mariane@yahoo.com.br.
authors.forename: Bruna
authors.initials: B
authors.lastname: Mariane
authors.affiliation: Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, 09972-270 Diadema, SP, Brazil. joao.lago@unifesp.br.
authors.forename: João Henrique G
authors.initials: JH
authors.lastname: Lago
authors.affiliation: Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, 09972-270 Diadema, SP, Brazil. patty.sart@unifesp.br.
authors.forename: Patricia
authors.initials: P
authors.lastname: Sartorelli
authors.affiliation: Instituto de Química, Universidade Federal de Alfenas, 37130-000 Alfenas, MG, Brazil. welton_rosa@hotmail.com.
authors.forename: Welton
authors.initials: W
authors.lastname: Rosa
authors.affiliation: Instituto de Química, Universidade Federal de Alfenas, 37130-000 Alfenas, MG, Brazil. marisigs@gmail.com.
authors.forename: Marisi G
authors.initials: MG
authors.lastname: Soares
authors.affiliation: Departamento de Química, Universidade Federal de Viçosa, 36570-000 Viçosa, MG, Brazil. adalbert31@hotmail.com.
authors.forename: Adalberto M
authors.initials: AM
authors.lastname: da Silva
authors.affiliation: Instituto Plantarum de Estudos da Flora. Av. Brasil, 2000, 13460-000 Nova Odessa, SP, Brazil. hlorenzi@plantarum.com.br.
authors.forename: Harri
authors.initials: H
authors.lastname: Lorenzi
authors.affiliation: Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, 09972-270 Diadema, SP, Brazil. marcelo.vallim@gmail.com.
authors.forename: Marcelo A
authors.initials: MA
authors.lastname: Vallim
authors.affiliation: Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, 09972-270 Diadema, SP, Brazil. renata.pascon@gmail.com.
authors.forename: Renata C
authors.initials: RC
authors.lastname: Pascon
mesh_terms: D000890:Anti-Infective Agents / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology*; D019659:Asteraceae / Q000378:metabolism*; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development; D001938:Brazil; D008401:Gas Chromatography-Mass Spectrometry; D008826:Microbial Sensitivity Tests; D009822:Oils, Volatile / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000378:metabolism; D010938:Plant Oils / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology*; D031346:Plectranthus / Q000378:metabolism*; D015003:Yeasts / Q000187:drug effects / Q000254:growth & development
chemical_list: D000890:Anti-Infective Agents; D009822:Oils, Volatile; D010938:Plant Oils
keywords: Eremanthus erythropappus; Plectranthus amboinicus; Plectranthus barbatus; antimicrobial activity; chemical composition; essential oils