title: Assessment of heavy metals and metalloids in tissues of two frog species: Rana tigrina and Euphlyctis cyanophlyctis from industrial city Sialkot, Pakistan.
abstract: In the present study, we investigated the concentrations of Ni, Fe, Pb, Cu, Co, Zn, Cd, Mn, and Cr in selected body tissues (liver, stomach, kidney, heart, lungs, and skeletal muscles) of two frog species: Rana tigrina and Euphlyctis cyanophlyctis captured from industrial wastewater of Sialkot city known worldwide for its tanning industry. The both frog species had darker appearance, distinctively different wet body weight, and snout-vent length. The results revealed that the heavy metal concentrations were high in the samples collected from industrial sites as compared to non-industrial sites. The different tissues of R. tigrina and E. cyanophlyctis exhibited little significant differences from two sites. The concentrations of heavy metals were more in tissues of R. tigrina as compared to E. cyanophlyctis. Mean concentration of Cd, Fe, Ni, Mn, Cu, and Cr was comparatively greater in R. tigrina, whereas Pb and Co were higher in E. cyanophlyctis. The concentration of Cu and Cd in the liver and kidney were relatively more in both species as compared to other organs. Further, the results indicated that frogs collected from industrial sites showed decreased body length and weight, and greater metal accumulation. The results will help the authorities for the conservation of these frog species which are under the influence of heavy metal contamination.
authors.affiliation: Laboratory of Animal and Human Physiology, Department of Animal Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan, irfanzia@qau.edu.pk.
authors.forename: Irfan Zia
authors.initials: IZ
authors.lastname: Qureshi
authors.forename: Zeshan
authors.initials: Z
authors.lastname: Kashif
authors.forename: Muhammad Zaffar
authors.initials: MZ
authors.lastname: Hashmi
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Su
authors.forename: Riffat Naseem
authors.initials: RN
authors.lastname: Malik
authors.forename: Kalim
authors.initials: K
authors.lastname: Ullah
authors.forename: Jinxing
authors.initials: J
authors.lastname: Hu
authors.forename: Muhammad
authors.initials: M
authors.lastname: Dawood
mesh_terms: D000818:Animals; D001001:Anura; D002947:Cities; D004784:Environmental Monitoring; D007221:Industry; D058955:Metalloids / Q000032:analysis*; D019216:Metals, Heavy / Q000032:analysis*; D010154:Pakistan; D062065:Wastewater / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D058955:Metalloids; D019216:Metals, Heavy; D062065:Wastewater; D014874:Water Pollutants, Chemical