title: Screening of molecular markers linked to dwarf trait in crape myrtle by bulked segregant analysis.
abstract: Plant height is one of the most important traits of plant architecture as it modulates both economic and ornamental values. Crape myrtle (Lagerstroemia indica L.) is a popular ornamental woody plant because of its long-lasting mid-summer bloom, rich colors, and diversified plant architecture. These traits also make it an ideal model of woody species for genetic analysis of many ornamental traits. To understand the inheritance of plant height and screen for genes modulating plant height in Lagerstroemia, segregation of the plant height trait was analyzed using the F1 population of L. fauriei (standard) x L. indica 'Pocomoke' (dwarf) with 96 seedlings, while dwarf genes were screened using the bulked segregant analysis method, combined with 28 amplified fragment length polymorphism primers and 41 simple sequence repeat primers. The results showed that the dwarf trait of crape myrtle was controlled by a major gene and modified by minor genes. An amplified fragment length polymorphism marker, M53E39-92, which was 23.33 cM from the loci controlling the dwarf trait, was screened. These results provide basic information for marker-assisted selection in Lagerstromia and cloning of dwarf genes in future studies.
authors.affiliation: Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture and College of Landscape Architecture, Beijing Forestry University, Beijing, China.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Ye
authors.affiliation: Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture and College of Landscape Architecture, Beijing Forestry University, Beijing, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture and College of Landscape Architecture, Beijing Forestry University, Beijing, China.
authors.forename: M
authors.initials: M
authors.lastname: Cai
authors.affiliation: Department of Ornamental Horticulture, College of Horticulture, Henan Agricultural University, Zhengzhou, China.
authors.forename: D
authors.initials: D
authors.lastname: He
authors.affiliation: Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture and College of Landscape Architecture, Beijing Forestry University, Beijing, China.
authors.forename: J S
authors.initials: JS
authors.lastname: Shen
authors.affiliation: Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture and College of Landscape Architecture, Beijing Forestry University, Beijing, China.
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Ju
authors.affiliation: Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture and College of Landscape Architecture, Beijing Forestry University, Beijing, China.
authors.forename: X M
authors.initials: XM
authors.lastname: Bian
authors.affiliation: Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture and College of Landscape Architecture, Beijing Forestry University, Beijing, China htpan@bjfu.edu.cn.
authors.forename: H T
authors.initials: HT
authors.lastname: Pan
authors.affiliation: Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture and College of Landscape Architecture, Beijing Forestry University, Beijing, China.
authors.forename: Q X
authors.initials: QX
authors.lastname: Zhang
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D001699:Biometry; D017343:Genes, Plant; D005819:Genetic Markers; D031563:Lagerstroemia / Q000033:anatomy & histology* / Q000235:genetics; D010641:Phenotype
chemical_list: D005819:Genetic Markers