title: Ethanol Extract of Peanut Sprout Lowers Blood Triglyceride Levels, Possibly Through a Pathway Involving SREBP-1c in Rats Fed a High-Fat Diet.
abstract: The hypothesis of this study was that peanut sprout extracts (PSE) could reduce fat accumulation through activating the transcription of SREBP-1c genes. Sprague-Dawley (SD) were randomly assigned into two groups and fed the following diet for 4 weeks; 10 normal fat (NF, 7 g of fat/100 g diet) and 30 high fat (HF, 20 g of fat/100 g diet). After 4 weeks, the HF group was divided into three groups; HF, HF with 15 mg of PSE/kg diet (HF+low PSE, 0.025% resveratrol), and HF with 30 mg of PSE/kg diet (HF+high PSE, 0.05% resveratrol) and fed for an additional 5 weeks. The HF+high PSE group had significantly lower weight gain than the HF group. Plasma triglyceride (TG) level and the hepatic total lipid level were significantly lower in the HF+high PSE group compared to the HF group. Fecal excretions of total lipids, cholesterol, and TG in the HF+high PSE group tended to be higher than in the HF group, but these differences were not significant. The mRNA expressions of fatty acid synthase, glucose-6-phosphate dehydrogenase, and sterol regulatory element binding protein-c (SREBP-1c) were significantly lower in the HF+high PSE group than in the HF group. The mRNA expressions of hydroxy-3-methylglutaryl coenzyme A reductase and acyl-CoA cholesterol acyltransferase were significantly lower in the HF+high PSE groups compared to the HF group. The mRNA expression of cholesterol 7α-hydroxylase1 was significantly higher than the HF group in both the HF+low PSE and HF+high PSE groups, with much greater increase observed in the HF+high PSE group. In conclusion, consumption of PSE was effective for improving blood lipid levels, possibly by suppressing the expression of SREBP-1c, in rats fed a high-fat diet.
authors.affiliation: 1 Department of Food Science and Nutrition, Dankook University , Gyeonggi, Korea.
authors.forename: Ae Wha
authors.initials: AW
authors.lastname: Ha
authors.affiliation: 2 Department of Food and Nutrition, Eulji University , Gyeonggi, Korea.
authors.forename: Nam E
authors.initials: NE
authors.lastname: Kang
authors.affiliation: 1 Department of Food Science and Nutrition, Dankook University , Gyeonggi, Korea.
authors.forename: Woo Kyoung
authors.initials: WK
authors.lastname: Kim
mesh_terms: D000818:Animals; D010367:Arachis / Q000737:chemistry*; D001835:Body Weight / Q000187:drug effects; D002784:Cholesterol / Q000097:blood / Q000378:metabolism; D059305:Diet, High-Fat / Q000009:adverse effects*; D004195:Disease Models, Animal; D000431:Ethanol / Q000737:chemistry; D050356:Lipid Metabolism / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D050177:Overweight / Q000178:diet therapy* / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D036226:Seedlings / Q000737:chemistry; D051780:Sterol Regulatory Element Binding Protein 1 / Q000378:metabolism*; D016896:Treatment Outcome; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D010936:Plant Extracts; D051780:Sterol Regulatory Element Binding Protein 1; D014280:Triglycerides; D000431:Ethanol; D002784:Cholesterol
keywords: CYP7A1; SREBP-1c; high-fat diet; peanut sprout; triglyceride