title: Exploitation of the complexation reaction of ortho-dihydroxylated anthocyanins with aluminum(III) for their quantitative spectrophotometric determination in edible sources.
abstract: Anthocyanins are natural pigments known for their color and antioxidant activity. These properties allow their use in various fields, including food and pharmaceutical ones. Quantitative determination of anthocyanins had been performed by non-specific methods that limit the accuracy and reliability of the results. Therefore, a novel, simple spectrophotometric method for the anthocyanins quantification based on a formation of blue-colored complexes by the known reaction between catechol- and pyrogallol-containing anthocyanins and aluminum(III) is presented. The method demonstrated to be reproducible, repetitive (RSD<1.5%) and highly sensitive to ortho-dihydroxylated anthocyanins (LOD = 0.186 μg/mL). Compliance with Beer's law was also evident in a range of concentrations (2-16 μg/mL for cyanidin 3-O-glucoside). Good recoveries (98.8-103.3%) were calculated using anthocyanin-rich plant samples. The described method revealed direct correlation to pH differential method results for several common anthocyanin-containing fruits indicating its great analytical potential. The presented method was successfully validated.
authors.affiliation: Laboratorio de Química Bioorgánica, InQuiBio, Departamento de Química, Facultad de Ciencias Básicas y Aplicadas, Universidad Militar Nueva Granada, Cundinamarca, Colombia.
authors.forename: Freddy A
authors.initials: FA
authors.lastname: Bernal
authors.affiliation: Laboratorio de Química Bioorgánica, InQuiBio, Departamento de Química, Facultad de Ciencias Básicas y Aplicadas, Universidad Militar Nueva Granada, Cundinamarca, Colombia.
authors.forename: Luisa L
authors.initials: LL
authors.lastname: Orduz-Diaz
authors.affiliation: Laboratorio de Química Bioorgánica, InQuiBio, Departamento de Química, Facultad de Ciencias Básicas y Aplicadas, Universidad Militar Nueva Granada, Cundinamarca, Colombia. Electronic address: ericsson.coy@unimilitar.edu.co.
authors.forename: Ericsson
authors.initials: E
authors.lastname: Coy-Barrera
mesh_terms: D000535:Aluminum / Q000737:chemistry*; D000872:Anthocyanins / Q000032:analysis / Q000737:chemistry*; D003116:Color; D005504:Food Analysis; D005960:Glucosides / Q000032:analysis; D057230:Limit of Detection; D015203:Reproducibility of Results; D013053:Spectrophotometry
chemical_list: D000872:Anthocyanins; D005960:Glucosides; C462279:cyanidin-3-O-beta-glucopyranoside; D000535:Aluminum
keywords: Aluminum(III); Anthocyanins; Spectrophotometric method; ortho-Dihydroxylated