title: One-dimensional proteomic profiling of Danio rerio embryo vitellogenin to estimate quantum dot toxicity.
abstract: BACKGROUND
  Vitellogenin (Vtg) is the major egg yolk protein (YP) in most oviparous species and may be useful as an indicator in ecotoxicological testing at the biochemical level. In this study, we obtained detailed information about the Vtgs of Danio rerio embryos by cutting SDS-PAGE gel lanes into thin slices, and analyzing them slice-by-slice with (MALDI-TOF) mass spectrometry.
  
  
  RESULTS
  We conducted three proteomic analyses, comparing embryonic Danio rerio Vtg cleavage products after exposure for 48 h to CdSecore/ZnSshell quantum dots (QDs), after exposure to a mixture of the components used for quantum dot synthesis (MCS-QDs), and in untreated embryos. The Vtg mass spectrometric profiles of the QDs-treated embryos differed from those of the unexposed or MCS-QDs-treated embryos.
  
  
  CONCLUSIONS
  This study demonstrates the possible utility of Vtg profiling in D. rerio embryos as a sensitive diagnostic tool to estimate nanoparticle toxicity.
authors.affiliation: Orekhovich Institute of Biomedical Chemistry, 119121, Pogodinskaya St. 10, Moscow, Russia ; Postgen Tech LLC, 119121, Pogodinskaya St. 10, Moscow, Russia.
authors.forename: Natalia A
authors.initials: NA
authors.lastname: Petushkova
authors.affiliation: Orekhovich Institute of Biomedical Chemistry, 119121, Pogodinskaya St. 10, Moscow, Russia.
authors.forename: Galina P
authors.initials: GP
authors.lastname: Kuznetsova
authors.affiliation: Orekhovich Institute of Biomedical Chemistry, 119121, Pogodinskaya St. 10, Moscow, Russia.
authors.forename: Olesya V
authors.initials: OV
authors.lastname: Larina
authors.affiliation: Orekhovich Institute of Biomedical Chemistry, 119121, Pogodinskaya St. 10, Moscow, Russia.
authors.forename: Yulia S
authors.initials: YS
authors.lastname: Kisrieva
authors.affiliation: Orekhovich Institute of Biomedical Chemistry, 119121, Pogodinskaya St. 10, Moscow, Russia.
authors.forename: Natalia F
authors.initials: NF
authors.lastname: Samenkova
authors.affiliation: Orekhovich Institute of Biomedical Chemistry, 119121, Pogodinskaya St. 10, Moscow, Russia.
authors.forename: Oxana P
authors.initials: OP
authors.lastname: Trifonova
authors.affiliation: Orekhovich Institute of Biomedical Chemistry, 119121, Pogodinskaya St. 10, Moscow, Russia.
authors.forename: Yuliana V
authors.initials: YV
authors.lastname: Miroshnichenko
authors.affiliation: Orekhovich Institute of Biomedical Chemistry, 119121, Pogodinskaya St. 10, Moscow, Russia.
authors.forename: Konstantin V
authors.initials: KV
authors.lastname: Zolotarev
authors.affiliation: Orekhovich Institute of Biomedical Chemistry, 119121, Pogodinskaya St. 10, Moscow, Russia.
authors.forename: Irina I
authors.initials: II
authors.lastname: Karuzina
authors.affiliation: Orekhovich Institute of Biomedical Chemistry, 119121, Pogodinskaya St. 10, Moscow, Russia.
authors.forename: Olga M
authors.initials: OM
authors.lastname: Ipatova
authors.affiliation: Orekhovich Institute of Biomedical Chemistry, 119121, Pogodinskaya St. 10, Moscow, Russia.
authors.forename: Andrey V
authors.initials: AV
authors.lastname: Lisitsa
keywords: Danio rerio embryos; MALDI-TOF mass spectrometry; Quantum dot; SDS-PAGE; Vitellogenin cleavage product