title: Ferruginibacter paludis sp. nov., isolated from wetland freshwater, and emended descriptions of Ferruginibacter lapsinanis and Ferruginibacter alkalilentus.
abstract: A Gram-staining-negative, aerobic and rod-shaped bacterium, designated HME8881T, was isolated from a freshwater wetland located in the Republic of Korea. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain HME8881T grouped with members of the genus Ferruginibacter. The most closely related species were Ferruginibacter lapsinanis HU1-HG42T (95.6%), Ferruginibacter alkalilentus HU1-GD23T (95.1%), 'Ferruginibacter profundus' DS48-5-3 (94.7%) and Ferruginibacter yonginensis HME8442T (93.4%). The major fatty acids were iso-C15 : 0, summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 1G, C16 : 0 and iso-C17 : 0 3-OH. A polar lipid analysis revealed phosphatidylethanolamine, two unidentified aminolipids, three unidentified aminophospholipids, two unidentified phospholipids and two unidentified lipids. DNA G+C content was 37.7 mol%. These results suggest that strain HME8881T represents a novel species of the genus Ferruginibacter, for which the name Ferruginibacter paludis sp. nov., is proposed with the type strain HME8881T ( = KCTC 42121T = CECT 8366T). In addition, emended descriptions of Ferruginibacter lapsinanis and Ferruginibacter alkalilentus are also proposed on the basis of new data obtained during this study.
authors.affiliation: Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, Gyeonggi 449-791, Republic of Korea.
authors.forename: Heeyoung
authors.initials: H
authors.lastname: Kang
authors.affiliation: Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, Gyeonggi 449-791, Republic of Korea.
authors.forename: Haneul
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Biological Sciences, Inha University, Incheon 402-751, Republic of Korea.
authors.forename: Yochan
authors.initials: Y
authors.lastname: Joung
authors.affiliation: Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, Gyeonggi 449-791, Republic of Korea.
authors.forename: Tae Yong
authors.initials: TY
authors.lastname: Jang
authors.affiliation: Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, Gyeonggi 449-791, Republic of Korea.
authors.forename: Kiseong
authors.initials: K
authors.lastname: Joh
mesh_terms: D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005618:Fresh Water / Q000382:microbiology*; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry; D053833:Wetlands
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7