title: High-throughput and rapid fluorescent visualization sensor of urinary citrate by CdTe quantum dots.
abstract: In this paper, we have presented a novel CdTe quantum dots (QDs) based fluorescent sensor for visual and turn-on sensing of citrate in human urine samples. The europium ion (Eu(3+)) can lead to the fluorescence quenching of thioglycollic acid (TGA) modified CdTe QDs due to photoinduced electron transfer accompanied by the change of emission color from yellow to orange. Next, addition of citrate breaks the preformed assembly because citrate can replace the CdTe QDs, based on the fact that the Eu(3+) ion displays higher affinity with citrate than the CdTe QDs. Thus the photoinduced electron transfer is switched off, and the fluorescence emission of CdTe QDs is rapidly (within 5min) recovered, simultaneously, the orange emission color restores to yellow. Such proposed strategy may conveniently discriminate the patient of renal stone from normal person by naked eyes. In addition to visualization detection, the fluorescence responses can be used for well quantifying citrate in the range of 0.67-133μM. So, the present, simple, low-cost and visualized citrate fluorescence sensor has great potential in the applications for earlier screening in clinical detection.
authors.affiliation: Key Laboratory of Functional Molecular Solids, Ministry of Education, Anhui Key Laboratory of Chemo-Biosensing, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China. Electronic address: sjzhuo@mail.ahnu.edu.cn.
authors.forename: Shujuan
authors.initials: S
authors.lastname: Zhuo
authors.affiliation: Key Laboratory of Functional Molecular Solids, Ministry of Education, Anhui Key Laboratory of Chemo-Biosensing, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Gong
authors.affiliation: Key Laboratory of Functional Molecular Solids, Ministry of Education, Anhui Key Laboratory of Chemo-Biosensing, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Functional Molecular Solids, Ministry of Education, Anhui Key Laboratory of Chemo-Biosensing, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China. Electronic address: zhucq@mail.ahnu.edu.cn.
authors.forename: Changqing
authors.initials: C
authors.lastname: Zhu
mesh_terms: D000328:Adult; D002104:Cadmium; D019343:Citric Acid / Q000652:urine*; D005063:Europium / Q000737:chemistry; D005260:Female; D005453:Fluorescence; D057166:High-Throughput Screening Assays / Q000379:methods; D006801:Humans; D008297:Male; D046529:Microscopy, Electron, Transmission; D045663:Quantum Dots; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet; D013691:Tellurium; D013864:Thioglycolates / Q000737:chemistry; D016482:Urinalysis / Q000379:methods*; D055815:Young Adult
chemical_list: D013864:Thioglycolates; D002104:Cadmium; D019343:Citric Acid; D005063:Europium; C017487:2-mercaptoacetate; D013691:Tellurium
keywords: CdTe quantum dots; Citrate; Earlier screening; Sensor; Visual detection