title: Bufavirus in fecal specimens of patients with and without diarrhea in Thailand.
abstract: Bufavirus (BuV) was initially discovered in fecal samples from children with acute diarrhea. In this study, we determined the prevalence, distribution, and genotype(s) of BuV in Thailand. A total of 1,495 diarrheal and 741 non-diarrheal stool specimens were collected and analyzed. A portion of the NS1 gene of BuV was amplified by nested RT-PCR. Phylogenetic analysis was performed to classify the BuV strains found. We detected bufavirus (BuV) in diarrheal (4/1495; 0.27%) but not in non-diarrheal specimens (0/726). All four strains belonged to BuV genotype 1. BuV could be detected in adults and children, but its role in causing acute diarrhea remains unclear.
authors.affiliation: Department of Pediatrics, Faculty of Medicine, Center of Excellence in Clinical Virology, Chulalongkorn University, Bangkok, 10330, Thailand.
authors.forename: Thaweesak
authors.initials: T
authors.lastname: Chieochansin
authors.forename: Viboonsak
authors.initials: V
authors.lastname: Vutithanachot
authors.forename: Apiradee
authors.initials: A
authors.lastname: Theamboonlers
authors.forename: Yong
authors.initials: Y
authors.lastname: Poovorawan
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D003967:Diarrhea / Q000453:epidemiology / Q000821:virology*; D005243:Feces / Q000821:virology*; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D010321:Parvoviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010322:Parvoviridae Infections / Q000453:epidemiology / Q000821:virology*; D010802:Phylogeny; D015995:Prevalence; D013785:Thailand / Q000453:epidemiology; D055815:Young Adult