title: BMP7 and EREG Contribute to the Inductive Potential of Dental Mesenchyme.
abstract: Odontogenesis is accomplished by reciprocal signaling between the epithelial and mesenchymal compartments. It is generally accepted that the inductive mesenchyme is capable of inducing the odontogenic commitment of both dental and non-dental epithelial cells. However, the duration of this signal in the developing dental mesenchyme and whether adult dental pulp tissue maintains its inductive capability remain unclear. This study investigated the contribution of growth factors to regulating the inductive potential of the dental mesenchyme. Human oral epithelial cells (OEs) were co-cultured with either human dental mesenchymal/papilla cells (FDPCs) or human dental pulp cells (ADPCs) under 2-dimensional or 3-dimensional conditions. Odontogenic-associated genes and proteins were detected by qPCR and immunofluorescence, respectively, and significant differences were observed between the two co-culture systems. The BMP7 and EREG expression levels in FDPCs were significantly higher than in ADPCs, as indicated by human growth factor PCR arrays and immunofluorescence analyses. OEs co-cultured with ADPCs supplemented with BMP7 and EREG expressed ameloblastic differentiation genes. Our study suggests that BMP7 and EREG expression in late bell-stage human dental papilla contributes to the inductive potential of dental mesenchyme. Furthermore, adult dental pulp cells supplemented with these two growth factors re-established the inductive potential of postnatal dental pulp tissue.
authors.affiliation: 1] State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, China [2] West China School of Stomatology, Sichuan University, Chengdu, Sichuan 610041, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Gao
authors.affiliation: 1] State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, China [2] Department of Orofacial Sciences, University of California, San Francisco, CA 94143, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.affiliation: 1] State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, China [2] West China School of Stomatology, Sichuan University, Chengdu, Sichuan 610041, China.
authors.forename: Xuedong
authors.initials: X
authors.lastname: Zhou
authors.affiliation: 1] State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, China [2] West China School of Stomatology, Sichuan University, Chengdu, Sichuan 610041, China.
authors.forename: Caixia
authors.initials: C
authors.lastname: Pi
authors.affiliation: 1] State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, China [2] West China School of Stomatology, Sichuan University, Chengdu, Sichuan 610041, China.
authors.forename: Ruoshi
authors.initials: R
authors.lastname: Xu
authors.affiliation: 1] State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, China [2] Department of Orofacial Sciences, University of California, San Francisco, CA 94143, USA.
authors.forename: Mian
authors.initials: M
authors.lastname: Wan
authors.affiliation: 1] State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, China [2] West China School of Stomatology, Sichuan University, Chengdu, Sichuan 610041, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: Center for Craniofacial Regeneration (CCR), College of Dental Medicine, Columbia University Medical Center, New York, NY 10032, USA.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: West China School of Stomatology, Sichuan University, Chengdu, Sichuan 610041, China.
authors.forename: Chengcheng
authors.initials: C
authors.lastname: Liu
authors.affiliation: West China School of Stomatology, Sichuan University, Chengdu, Sichuan 610041, China.
authors.forename: Jianxun
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of Orofacial Sciences, University of California, San Francisco, CA 94143, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: 1] State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, China [2] West China School of Stomatology, Sichuan University, Chengdu, Sichuan 610041, China.
authors.forename: Liwei
authors.initials: L
authors.lastname: Zheng
mesh_terms: D053523:Amelogenin / Q000235:genetics; D055419:Bone Morphogenetic Protein 7 / Q000494:pharmacology*; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D016000:Cluster Analysis; D018920:Coculture Techniques; D003746:Dental Enamel Proteins / Q000235:genetics; D003771:Dental Papilla / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D003782:Dental Pulp / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D066260:Epiregulin / Q000494:pharmacology*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D006801:Humans; D008648:Mesoderm / Q000166:cytology / Q000187:drug effects* / Q000196:embryology*; D009805:Odontogenesis / Q000187:drug effects*; D051764:PAX9 Transcription Factor / Q000235:genetics
chemical_list: C490889:AMBN protein, human; C507067:AMTN protein, human; D053523:Amelogenin; D055419:Bone Morphogenetic Protein 7; D003746:Dental Enamel Proteins; D066260:Epiregulin; D051764:PAX9 Transcription Factor; C497323:PAX9 protein, human