title: [Construction of genetically engineered strain producing 5- oxomilbemycin by knocking out milF in Steptomyces hygroscopicus HS023].
abstract: OBJECTIVE
  To construct a 5-oxomilbemycin producing strain by knocking out milF gene in Streptomyces hygroscopicus HS023.
  
  
  METHODS
  Plasmid pMSST-ΔmilF was constructed and introduced into milbemycin industrial strain Streptomyces hygroscopicus HS023, and milF mutant F2-18 was selected by PCR amplification.
  
  
  RESULTS
  Fermentation experiments showed that no milbemycins was produced in F2-18, but 5-ketomilbemycin, the intermediate of milbemycin, was obviously accumulated, and the fermentation titer was enhanced.
  
  
  CONCLUSIONS
  Genetically engineered strain can simplify the synthesis of milbemycin oxime and lepimectin chemical from milbemycin.
authors.forename: Jun
authors.initials: J
authors.lastname: Huang
authors.forename: Jiatan
authors.initials: J
authors.lastname: Lin
authors.forename: Min
authors.initials: M
authors.lastname: Zhou
authors.forename: Hua
authors.initials: H
authors.lastname: Bai
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D017353:Gene Deletion; D055786:Gene Knockout Techniques; D005818:Genetic Engineering; D018942:Macrolides / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D018942:Macrolides; C078973:milbemycin oxime