title: Hair regrowth through wound healing process after ablative fractional laser treatment in a murine model.
abstract: OBJECTIVE
  Alopecia is one of the most common dermatological problems in the elderly; however, current therapies for it are limited by low efficacy and undesirable side effects. Although clinical reports on fractional laser treatment for various alopecia types are increasing, the exact mechanism remains to be clarified. The purposes of this study were to demonstrate the effect of ablative fractional laser treatment on hair follicle regrowth in vivo and investigate the molecular mechanism after laser treatment.
  
  
  METHODS
  Ablative CO2 fractional laser was applied to the shaved dorsal skin of 7-week-old C57BL/6 mice whose hair was in the telogen stage. After 12 mice were treated at various energy (10-40 mJ/spot) and density (100-400 spots/cm(2) ) settings to determine the proper dosage for maximal effect. Six mice were then treated at the decided dosage and skin specimens were sequentially obtained by excision biopsy from the dorsal aspect of each mouse. Tissue samples were used for the immunohistochemistry and reverse transcription polymerase chain reaction assays to examine hair follicle status and their related molecules.
  
  
  RESULTS
  The most effective dosage was the 10 mJ/spot and 300 spots/cm(2) setting. The anagen conversion of hair was observed in the histopathological examination, while Wnt/β-catenin expression was associated with hair regrowth in the immunohistochemistry and molecular studies.
  
  
  CONCLUSIONS
  Ablative fractional lasers appear to be effective for inducing hair regrowth via activation of the Wnt/β-catenin pathway in vivo. Our findings indicate that fractional laser treatment can potentially be developed as new treatment options for stimulating hair regrowth.
authors.affiliation: Department of Dermatology, St. Vincent's Hospital, College of Medicine, The Catholic University of Korea, Suwon, Korea.
authors.forename: Jung Min
authors.initials: JM
authors.lastname: Bae
authors.affiliation: Department of Dermatology, St. Vincent's Hospital, College of Medicine, The Catholic University of Korea, Suwon, Korea.
authors.forename: Han Mi
authors.initials: HM
authors.lastname: Jung
authors.affiliation: Clinique L, Goyang, Korea.
authors.forename: Boncheol
authors.initials: B
authors.lastname: Goo
authors.affiliation: Department of Dermatology, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.
authors.forename: Young Min
authors.initials: YM
authors.lastname: Park
mesh_terms: D000505:Alopecia / Q000378:metabolism / Q000628:therapy*; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D006197:Hair / Q000254:growth & development* / Q000528:radiation effects*; D054020:Lasers, Gas / Q000627:therapeutic use*; D051379:Mice; D008810:Mice, Inbred C57BL; D051153:Wnt Proteins / Q000378:metabolism; D014945:Wound Healing / Q000502:physiology; D051176:beta Catenin / Q000378:metabolism
chemical_list: D051153:Wnt Proteins; C102068:Wnt10b protein, mouse; D051176:beta Catenin
keywords: alopecia; androgenic alopecia; fractional laser; hair follicle; hair loss; laser