title: Cutting edge: Bcl6-interacting corepressor contributes to germinal center T follicular helper cell formation and B cell helper function.
abstract: CD4(+) germinal center (GC)-T follicular helper (Tfh) cells help B cells become long-lived plasma cells and memory cells. The transcriptional repressor Bcl6 plays a key role in GC-Tfh formation by inhibiting the expression of genes that promote differentiation into other lineages. We determined whether BCOR, a component of a Polycomb repressive complex that interacts with the Bcl6 BTB domain, influences GC-Tfh differentiation. T cell-targeted BCOR deficiency led to a substantial loss of peptide:MHC class II-specific GC-Tfh cells following Listeria monocytogenes infection and a 2-fold decrease following immunization with a peptide in CFA. The reduction in GC-Tfh cells was associated with diminished plasma cell and GC B cell formation. Thus, T cell-expressed BCOR is critical for optimal GC-Tfh cell differentiation and humoral immunity.
authors.affiliation: Department of Microbiology and Immunology, Center for Immunology, University of Minnesota Medical School, Minneapolis, MN 55455; and.
authors.forename: Jessica A
authors.initials: JA
authors.lastname: Yang
authors.affiliation: Department of Microbiology and Immunology, Center for Immunology, University of Minnesota Medical School, Minneapolis, MN 55455; and.
authors.forename: Noah J
authors.initials: NJ
authors.lastname: Tubo
authors.affiliation: Developmental Biology Center, Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455; and University of Minnesota Masonic Cancer Center, Minneapolis, MN 55455.
authors.forename: Micah D
authors.initials: MD
authors.lastname: Gearhart
authors.affiliation: Developmental Biology Center, Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455; and University of Minnesota Masonic Cancer Center, Minneapolis, MN 55455.
authors.forename: Vivian J
authors.initials: VJ
authors.lastname: Bardwell
authors.affiliation: Department of Microbiology and Immunology, Center for Immunology, University of Minnesota Medical School, Minneapolis, MN 55455; and jenki002@umn.edu.
authors.forename: Marc K
authors.identifier: http://orcid.org/0000-0001-8009-7655
authors.initials: MK
authors.lastname: Jenkins
mesh_terms: D000818:Animals; D016175:B-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism*; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D005260:Female; D018858:Germinal Center / Q000276:immunology* / Q000378:metabolism*; D016130:Immunophenotyping; D008213:Lymphocyte Activation / Q000235:genetics / Q000276:immunology; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D010641:Phenotype; D012097:Repressor Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*
chemical_list: C553467:Bcor protein, mouse; D012097:Repressor Proteins