title: Motilin stimulates pepsinogen secretion in Suncus murinus.
abstract: Motilin and ghrelin are gastrointestinal hormones that stimulate the migrating motor complex (MMC) of gastrointestinal motility during the fasting state. In this study, we examined the effect of motilin and ghrelin on pepsinogen secretion in anesthetized suncus (house musk shrew, Suncus murinus), a ghrelin- and motilin-producing mammal. By using a gastric lumen-perfusion system, we found that the intravenous administration of carbachol and motilin stimulated pepsinogen secretion, the latter in a dose-dependent manner, whereas ghrelin had no effect. We then investigated the pathways of motilin-induced pepsinogen secretion using acetylcholine receptor antagonists. Treatment with atropine, a muscarinic acetylcholine receptor antagonist, completely inhibited both carbachol and motilin-induced pepsinogen secretion. Motilin-induced pepsinogen secretion was observed in the vagotomized suncus. This is the first report demonstrating that motilin stimulates pepsinogen secretion, and suggest that this effect occurs through a cholinergic pathway in suncus.
authors.affiliation: Area of Regulatory Biology, Division of Life Science, Graduate School of Science and Engineering, Saitama University, Saitama, Japan.
authors.forename: Chayon
authors.initials: C
authors.lastname: Goswami
authors.affiliation: Faculty of Pharmaceutical Sciences, Department of Pharmaceutical and Health Sciences, Josai University, Saitama, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Tanaka
authors.affiliation: Laboratory of Animal Management and Resources, Department of Zoology, Faculty of Science, Okayama University of Science, Okayama, Japan.
authors.forename: Takamichi
authors.initials: T
authors.lastname: Jogahara
authors.affiliation: Area of Regulatory Biology, Division of Life Science, Graduate School of Science and Engineering, Saitama University, Saitama, Japan.
authors.forename: Takafumi
authors.initials: T
authors.lastname: Sakai
authors.affiliation: Area of Regulatory Biology, Division of Life Science, Graduate School of Science and Engineering, Saitama University, Saitama, Japan. Electronic address: isakata@mail.saitama-u.ac.jp.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Sakata
mesh_terms: D000818:Animals; D001285:Atropine / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D054439:Ghrelin / Q000008:administration & dosage; D007275:Injections, Intravenous; D008297:Male; D009037:Motilin / Q000008:administration & dosage / Q000494:pharmacology* / Q000502:physiology*; D018727:Muscarinic Antagonists / Q000494:pharmacology; D020116:Pepsinogen A / Q000378:metabolism*; D012788:Shrews / Q000502:physiology*; D014628:Vagotomy
chemical_list: D054439:Ghrelin; D018727:Muscarinic Antagonists; D009037:Motilin; D001285:Atropine; D002217:Carbachol; D020116:Pepsinogen A
keywords: Gastric acid; Ghrelin; Motilin; Pepsinogen; Suncus