title: Cell toxicity of 2-hydroxyethyl methacrylate (HEMA): the role of oxidative stress.
abstract: 2-Hydroxyethyl methacrylate (HEMA) is a methacrylate monomer used in polymer-based dental-restorative materials. In this study, the viability of human lung epithelial cells, BEAS-2B, was investigated after exposure to this monomer. Exposure to HEMA reduced the viability of the BEAS-2B cells as a result of increased apoptosis, interruption of the cell cycle, and decreased cell proliferation. Depletion of cellular glutathione and increased levels of reactive oxygen species (ROS) were seen after exposure of BEAS-2B cells to HEMA. The glutathione synthase inhibitor, L-buthioninesulfoximine (BSO), was used to study whether the reduced viability was caused by glutathione depletion and increased levels of ROS. Similarly to incubation with HEMA, incubation with BSO resulted in glutathione depletion and increased ROS levels, without increasing cell death or inhibiting cell growth. The results indicate that HEMA-induced cell damage is not caused exclusively by these mechanisms. Mechanisms other than glutathione depletion and ROS formation seem to be of importance for the toxic effect of HEMA on lung epithelial cells.
authors.affiliation: Nordic Institute of Dental Materials, Oslo, Norway.
authors.forename: Else
authors.initials: E
authors.lastname: Morisbak
authors.affiliation: Nordic Institute of Dental Materials, Oslo, Norway.|Department of Clinical Dentistry, Faculty of Medicine and Dentistry, University of Bergen, Bergen, Norway.
authors.forename: Vibeke
authors.initials: V
authors.lastname: Ansteinsson
authors.affiliation: Nordic Institute of Dental Materials, Oslo, Norway.
authors.forename: Jan T
authors.initials: JT
authors.lastname: Samuelsen
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D019328:Buthionine Sulfoximine / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004847:Epithelial Cells / Q000187:drug effects; D005978:Glutathione / Q000187:drug effects; D005981:Glutathione Synthase / Q000037:antagonists & inhibitors; D006801:Humans; D008168:Lung / Q000166:cytology / Q000187:drug effects*; D008422:Materials Testing; D008689:Methacrylates / Q000633:toxicity*; D018384:Oxidative Stress / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000032:analysis
chemical_list: D004791:Enzyme Inhibitors; D008689:Methacrylates; D017382:Reactive Oxygen Species; D019328:Buthionine Sulfoximine; C005044:hydroxyethyl methacrylate; D005981:Glutathione Synthase; D005978:Glutathione
keywords: adhesives; apoptosis; cell proliferation; glutathione; toxicology