title: MicroRNA-133a improves the cardiac function and fibrosis through inhibiting Akt in heart failure rats.
abstract: OBJECTIVE
  MicroRNAs (miRNAs), a group of small non-coding RNAs that fine tune translation of multiple target mRNAs, have been implicated in the development and progression of heart failure.
  
  
  METHODS
  The present study was undertaken to determine the roles of miR-133a on the anatomical, hemodynamic and fibrosis of heart in the chronic heart failure rats, and the downstream signaling pathway.
  
  
  RESULTS
  The expression of miR-133a in the heart of chronic heart failure from patients or rats was decreased. The miR-133a mimic and miR-133a overexpression caused a decrease in the heart weight/body weight (HW/BW) and LVEDP, and an increase in the LVSP and +LV dP/dt(max) in the chronic heart failure rats. However, the miR-133a inhibitor promoted the HW/BW and LVEDP, and caused a decrease in the LVSP and LV dP/dt(max) in the chronic heart failure rats. The miR-133a mimic and miR-133a overexpression significantly caused a decrease in the fibrosis of heart in chronic heart failure rats. The Akt inhibitor TCN abolished the effects of miR-133a on the HW/BW and LVEDP decrease, LVSP and LV dP/dt(max) increase in the chronic heart failure rats. The miR-133a increased the expression of phosphorylated Akt in the heart of chronic heart failure rats.
  
  
  CONCLUSIONS
  These results demonstrated that miR-133a improves the cardiac function and fibrosis through inhibiting Akt in heart failure rats.
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, 450052 Henan, Zhengzhou, PR China. Electronic address: haiqiangsang@163.com.
authors.forename: Hai-Qiang
authors.initials: HQ
authors.lastname: Sang
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, 450052 Henan, Zhengzhou, PR China.
authors.forename: Zheng-Ming
authors.initials: ZM
authors.lastname: Jiang
authors.affiliation: Department of hypertension, Henan Provincial People's Hospital, 450003 Henan, Zhengzhou, PR China.
authors.forename: Qiu-Ping
authors.initials: QP
authors.lastname: Zhao
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, 450052 Henan, Zhengzhou, PR China.
authors.forename: Fu
authors.initials: F
authors.lastname: Xin
mesh_terms: D000818:Animals; D001835:Body Weight; D002908:Chronic Disease; D005355:Fibrosis; D005786:Gene Expression Regulation / Q000187:drug effects; D006333:Heart Failure / Q000201:enzymology / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology*; D006334:Heart Function Tests; D006439:Hemodynamics / Q000187:drug effects / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009929:Organ Size; D010766:Phosphorylation / Q000187:drug effects; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D051057:Proto-Oncogene Proteins c-akt / Q000037:antagonists & inhibitors* / Q000378:metabolism; D051381:Rats; D012263:Ribonucleosides / Q000494:pharmacology
chemical_list: C548234:MIRN133 microRNA, human; C549446:MIRN133 microRNA, rat; D035683:MicroRNAs; D047428:Protein Kinase Inhibitors; D012263:Ribonucleosides; C023764:triciribine; D051057:Proto-Oncogene Proteins c-akt
keywords: Akt; Fibrosis; Heart failure; miR-133a