title: MicroRNA-145 suppresses cell migration and invasion by targeting paxillin in human colorectal cancer cells.
abstract: A number of cancers show increased expression of paxillin which plays a central role in tumor progression, including colorectal cancer. However, the mechanisms causing paxillin upregulation remains unclear. In our study, bioinformatics analyses suggested that paxillin is predicted to be a direct target of miR-145. We firstly identified paxillin as a new target of miR-145 and demonstrated that miR-145 inhibits paxillin expression by binding to the paxillin mRNA 3'UTR. Therefore, we assume overexpression of paxillin induced by suppression of miR-145 may promote cell migration and invasion. We detected the expression of paxillin and miR-145 in human colorectal cancer tissues by real-time quantitative PCR. Higher expression of paxillin and lower expression of miR-145 was observed in colorectal cancer tissues than corresponding paracancerous tissue. Moreover, the expression of paxillin was negatively correlated with miR-145 expression. A dual-luciferase reporter assay was used to confirm that paxillin was a direct target of miR-145. In CRC cell lines, overexpression of miR-145 could downregulate paxillin protein expression levels, and ectopic overexpression of miR-145 mimics or inhibitor could inhibit or promote cell migration, invasion, proliferation and clone formation in vitro. Taken together, these data suggested that miR-145 plays a pivotal role in colon cancer through inhibiting cell proliferation migration and invasion, and miR-145 may serve as a tumor suppressor by targeting paxillin gene.
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University Nantong 226001, Jiangsu Province, P. R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Qin
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University Nantong 226001, Jiangsu Province, P. R. China.
authors.forename: Feiran
authors.initials: F
authors.lastname: Wang
authors.affiliation: Medical College of Nantong University Nantong 226001, Jiangsu Province, P. R. China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University Nantong 226001, Jiangsu Province, P. R. China.
authors.forename: Junfei
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University Nantong 226001, Jiangsu Province, P. R. China.
authors.forename: Yasu
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University Nantong 226001, Jiangsu Province, P. R. China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Wang
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009361:Neoplasm Invasiveness / Q000235:genetics* / Q000473:pathology; D051419:Paxillin / Q000378:metabolism*
chemical_list: C541010:MIRN145 microRNA, human; D035683:MicroRNAs; D051419:Paxillin
keywords: colorectal cancer; miR-145; migration; paxillin; proliferation