title: Crystal structure of Cry51Aa1: A potential novel insecticidal aerolysin-type β-pore-forming toxin from Bacillus thuringiensis.
abstract: The structures of several Bacillus thuringiensis (Bt) insecticidal crystal proteins have been determined by crystallographic methods and a close relationship has been explicated between specific toxicities and conserved three-dimensional architectures. In this study, as a representative of the coleopteran- and hemipteran-specific Cry51A group, the complete structure of Cry51Aa1 protoxin has been determined by X-ray crystallography at 1.65 Å resolution. This is the first report of a coleopteran-active Bt insecticidal toxin with high structural similarity to the aerolysin-type β-pore forming toxins (β-PFTs). Moreover, study of featured residues and structural elements reveal their possible roles in receptor binding and pore formation events. This study provides new insights into the action of aerolysin-type β-PFTs from a structural perspective, and could be useful for the control of coleopteran and hemipteran insect pests in agricultures.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, PR China; Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, 30602, USA.
authors.forename: Chengchen
authors.initials: C
authors.lastname: Xu
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, 30602, USA; SER-CAT, University of Georgia and the Advanced Photon Source, Argonne National Laboratory, Argonne, IL, 60439, USA.
authors.forename: Unmesh
authors.initials: U
authors.lastname: Chinte
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, 30602, USA.
authors.forename: Lirong
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Biochemistry, School of Life Sciences, Fudan University, Shanghai, 200438, PR China.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Yao
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Meng
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, 30602, USA.
authors.forename: Dayong
authors.initials: D
authors.lastname: Zhou
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, PR China.
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Bi
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, 30602, USA; SER-CAT, University of Georgia and the Advanced Photon Source, Argonne National Laboratory, Argonne, IL, 60439, USA.
authors.forename: John
authors.initials: J
authors.lastname: Rose
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, 30602, USA; Department of Entomology, University of Georgia, Athens, GA, 30602, USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Adang
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, 30602, USA; SER-CAT, University of Georgia and the Advanced Photon Source, Argonne National Laboratory, Argonne, IL, 60439, USA. Electronic address: wang@bcl1.bmb.uga.edu.
authors.forename: Bi-Cheng
authors.initials: BC
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, PR China. Electronic address: yz41@mail.hzau.edu.cn.
authors.forename: Ziniu
authors.initials: Z
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, PR China. Electronic address: m98sun@mail.hzau.edu.cn.
authors.forename: Ming
authors.initials: M
authors.lastname: Sun
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001413:Bacillus thuringiensis / Q000737:chemistry* / Q000235:genetics; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D001427:Bacterial Toxins / Q000737:chemistry* / Q000235:genetics; D001517:Coleoptera; D018360:Crystallography, X-Ray; D004731:Endotoxins / Q000737:chemistry* / Q000235:genetics; D006460:Hemolysin Proteins / Q000737:chemistry* / Q000235:genetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010572:Pest Control, Biological; D052899:Pore Forming Cytotoxic Proteins / Q000737:chemistry* / Q000235:genetics; D011487:Protein Conformation; D054730:Protein Interaction Domains and Motifs; D020836:Protein Structure, Quaternary; D017386:Sequence Homology, Amino Acid
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D052899:Pore Forming Cytotoxic Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis; C032676:aerolysin
keywords: Bacillus thuringiensis; Crystal structure; Insecticidal; Pore-forming toxin