title: Activatable Dendritic19F Probes for Enzyme Detection.
abstract: We describe a novel activatable probe for fluorine-19 NMR based on self-assembling amphiphilic dendrons. The dendron probe has been designed to be spectroscopically silent due to the formation of large aggregates. Upon exposure to the specific target enzyme, the aggregates disassemble to give rise to a sharp19F NMR signal. The probe is capable of detecting enzyme concentrations in the low nanomolar range. Response time of the probe was found to be affected by the hydrophilic-lipophilic balance of dendrons. Understanding the structural factors that underlie this design principle provides the pathway for using this strategy for a broad range of enzyme-based imaging.
authors.affiliation: Department of Chemistry, University of Massachusetts Amherst , Amherst, Massachusetts 01003, United States.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Chemistry, University of Massachusetts Amherst , Amherst, Massachusetts 01003, United States.
authors.forename: Krishna R
authors.initials: KR
authors.lastname: Raghupathi
authors.affiliation: Department of Chemistry, University of Massachusetts Amherst , Amherst, Massachusetts 01003, United States.
authors.forename: Jiaming
authors.initials: J
authors.lastname: Zhuang
authors.affiliation: Department of Chemistry, University of Massachusetts Amherst , Amherst, Massachusetts 01003, United States.
authors.forename: S
authors.initials: S
authors.lastname: Thayumanavan