title: First and second line drug resistance among treatment naïve pulmonary tuberculosis patients in a district under Revised National Tuberculosis Control Programme (RNTCP) in New Delhi.
abstract: There is limited information of level of drug resistance to first-line and second line anti-tuberculosis agents in treatment naïve pulmonary tuberculosis (PTB) patients from the Indian region. Therefore, the present prospective study was conducted to determine the antimicrobial susceptibility to first-line and second line anti-TB drug resistance in such patients. Sputum samples from consecutive treatment naïve PTB cases registered in Lala Ram Sarup (LRS) district, under RNTCP containing 12 Directly Observed Treatment Centre's (DOTS), were enrolled using cluster sampling technology. A total of 453 samples were received from July 2011 to June 2012. All samples were cultured on solid medium followed by drug susceptibility to first and second line anti-tubercular drugs as per RNTCP guidelines. Primary multi-drug resistance (MDR) was found to be 18/453; (4.0%). Extensively drug resistance (XDR) was found in one strain (0.2%), which was found to be resistant to other antibiotics. Data of drug resistant tuberculosis among treatment naïve TB patients are lacking in India. The presence of XDR-TB and high MDR-TB in small population studied, calls for conducting systematic multi-centric surveillance across the country.
authors.affiliation: National Reference Laboratory & WHO Center of Excellence (Tuberculosis), Department of Microbiology, National Institute of Tuberculosis and Respiratory Diseases, New Delhi 110030, India. Electronic address: tbmicro@gmail.com.
authors.forename: Vithal Prasad
authors.initials: VP
authors.lastname: Myneedu
authors.affiliation: National Reference Laboratory & WHO Center of Excellence (Tuberculosis), Department of Microbiology, National Institute of Tuberculosis and Respiratory Diseases, New Delhi 110030, India. Electronic address: ritugo@hotmail.com.
authors.forename: Ritu
authors.initials: R
authors.lastname: Singhal
authors.affiliation: Department of Epidemiology, National Institute of Tuberculosis and Respiratory Diseases, New Delhi 110030, India. Electronic address: dr.khaliduk@yahoo.com.
authors.forename: Khalid Umer
authors.initials: KU
authors.lastname: Khayyam
authors.affiliation: Department of Statistics, National Institute of Tuberculosis and Respiratory Diseases, New Delhi 110030, India. Electronic address: ppsharma@hotmail.com.
authors.forename: Prem Prakash
authors.initials: PP
authors.lastname: Sharma
authors.affiliation: National Reference Laboratory & WHO Center of Excellence (Tuberculosis), Department of Microbiology, National Institute of Tuberculosis and Respiratory Diseases, New Delhi 110030, India. Electronic address: mnprtbhalla@yahoo.com.
authors.forename: Manpreet
authors.initials: M
authors.lastname: Bhalla
authors.affiliation: Department of Pulmonary Medicine, Post Graduate Institute of Medical Education and Research, Chandigarh 160012, India. Electronic address: dirlrsi@gmail.com.
authors.forename: Digamber
authors.initials: D
authors.lastname: Behera
authors.affiliation: Department of Tuberculosis and Chest Diseases, National Institute of Tuberculosis and Respiratory Diseases, New Delhi 110030, India. Electronic address: tbmicro@gmail.com.
authors.forename: Rohit
authors.initials: R
authors.lastname: Sarin
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000995:Antitubercular Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002648:Child; D054908:Extensively Drug-Resistant Tuberculosis / Q000453:epidemiology*; D005260:Female; D006801:Humans; D007194:India / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D011446:Prospective Studies; D013183:Sputum / Q000382:microbiology; D018088:Tuberculosis, Multidrug-Resistant / Q000453:epidemiology*; D014397:Tuberculosis, Pulmonary / Q000188:drug therapy* / Q000517:prevention & control; D055815:Young Adult
chemical_list: D000995:Antitubercular Agents
keywords: Antibiotic; Extensively drug resistance; Multi-drug resistance; Primary; Pulmonary