title: Collagen/chitosan based two-compartment and bi-functional dermal scaffolds for skin regeneration.
abstract: Inspired from the sophisticated bilayer structures of natural dermis, here, we reported collagen/chitosan based two-compartment and bi-functional dermal scaffolds. Two functions refer to mediating rapid angiogenesis based on recombinant human vascular endothelial growth factor (rhVEGF) and antibacterial from gentamicin, which were encapsulated in PLGA microspheres. The gentamicin and rhVEGF encapsulated PLGA microspheres were further combined with collagen/chitosan mixtures in low (lower layer) and high (upper layer) concentrations, and molded to generate the two-compartment and bi-functional scaffolds. Based on morphology and pore structure analyses, it was found that the scaffold has a distinct double layered porous and connective structure with PLGA microspheres encapsulated. Statistical analysis indicated that the pores in the upper layer and in the lower layer have great variations in diameter, indicative of a two-compartment structure. The release profiles of gentamicin and rhVEGF exceeded 28 and 49 days, respectively. In vitro culture of mouse fibroblasts showed that the scaffold can facilitate cell adhesion and proliferation. Moreover, the scaffold can obviously inhibit proliferation of Staphylococcus aureus and Serratia marcescens, exhibiting its unique antibacterial effect. The two-compartment and bi-functional dermal scaffolds can be a promising candidate for skin regeneration.
authors.affiliation: Department of Plastic Surgery and Burns, Shenzhen Second People's Hospital, Shenzhen 518035, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.affiliation: Key Laboratory of Biomedical Materials and Implants, Research Institute of Tsinghua University in Shenzhen, Shenzhen 518057, PR China.
authors.forename: Mingbo
authors.initials: M
authors.lastname: Wang
authors.affiliation: Key Laboratory of Biomedical Materials and Implants, Research Institute of Tsinghua University in Shenzhen, Shenzhen 518057, PR China; Shenzhen Lando Biomaterials Co., Ltd., Shenzhen 518057, PR China.
authors.forename: Zhending
authors.initials: Z
authors.lastname: She
authors.affiliation: Department of Plastic Surgery and Burns, Shenzhen Second People's Hospital, Shenzhen 518035, PR China.
authors.forename: Kunwu
authors.initials: K
authors.lastname: Fan
authors.affiliation: Department of Plastic Surgery and Burns, Shenzhen Second People's Hospital, Shenzhen 518035, PR China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Xu
authors.affiliation: Key Laboratory of Biomedical Materials and Implants, Research Institute of Tsinghua University in Shenzhen, Shenzhen 518057, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Chu
authors.affiliation: Key Laboratory of Biomedical Materials and Implants, Research Institute of Tsinghua University in Shenzhen, Shenzhen 518057, PR China.
authors.forename: Changsheng
authors.initials: C
authors.lastname: Chen
authors.affiliation: The Burns Department of Zhujiang Hospital, Southern Medical University, Guangzhou 510280, PR China. Electronic address: shengjunshi@yahoo.com.
authors.forename: Shengjun
authors.initials: S
authors.lastname: Shi
authors.affiliation: Key Laboratory of Biomedical Materials and Implants, Research Institute of Tsinghua University in Shenzhen, Shenzhen 518057, PR China; Shenzhen Lando Biomaterials Co., Ltd., Shenzhen 518057, PR China. Electronic address: tanrw@landobiom.com.
authors.forename: Rongwei
authors.initials: R
authors.lastname: Tan
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan; D003094:Collagen; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D051379:Mice; D008863:Microspheres; D011100:Polyglycolic Acid / Q000737:chemistry; D012706:Serratia marcescens / Q000187:drug effects; D017768:Skin, Artificial; D013211:Staphylococcus aureus / Q000187:drug effects; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D042461:Vascular Endothelial Growth Factor A / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D011100:Polyglycolic Acid; D003094:Collagen; D048271:Chitosan
keywords: Antibacterial activity; Biomimetic dermal scaffold; Drug delivery; Skin regeneration; rhVEGF