title: An anti-G protein monoclonal antibody treats RSV disease more effectively than an anti-F monoclonal antibody in BALB/c mice.
abstract: Respiratory syncytial virus (RSV) belongs to the family Paramyxoviridae and is the single most important cause of serious lower respiratory tract infections in young children, yet no highly effective treatment or vaccine is available. To clarify the potential for an anti-G mAb, 131-2G which has both anti-viral and anti-inflammatory effects, to effectively treat RSV disease, we determined the kinetics of its effect compared to the effect of the anti-F mAb, 143-6C on disease in mice. Treatment administered three days after RSV rA2-line19F (r19F) infection showed 131-2G decreased breathing effort, pulmonary mucin levels, weight loss, and pulmonary inflammation earlier and more effectively than treatment with mAb 143-6C. Both mAbs stopped lung virus replication at day 5 post-infection. These data show that, in mice, anti-G protein mAb is superior to treating disease during RSV infection than an anti-F protein mAb similar to Palivizumab. This combination of anti-viral and anti-inflammatory activity makes 131-2G a promising candidate for treating for active human RSV infection.
authors.affiliation: Emory University Department of Pediatrics and Children׳s Healthcare of Atlanta, Atlanta, GA 30322, USA.
authors.forename: Seyhan
authors.initials: S
authors.lastname: Boyoglu-Barnum
authors.affiliation: Emory University Department of Pediatrics and Children׳s Healthcare of Atlanta, Atlanta, GA 30322, USA.
authors.forename: Sean O
authors.initials: SO
authors.lastname: Todd
authors.affiliation: Emory University Department of Pediatrics and Children׳s Healthcare of Atlanta, Atlanta, GA 30322, USA.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Chirkova
authors.affiliation: University of Georgia, Odum School of Ecology, Athens, GA 30602, USA.
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Barnum
authors.affiliation: Emory University Department of Pediatrics and Children׳s Healthcare of Atlanta, Atlanta, GA 30322, USA.
authors.forename: Kelsey A
authors.initials: KA
authors.lastname: Gaston
authors.affiliation: Division of Viral Diseases, NCIRD, CDC, Atlanta, GA 30333, USA.
authors.forename: Lia M
authors.initials: LM
authors.lastname: Haynes
authors.affiliation: University of Georgia Department of Infectious Diseases, Animal Health Research Center, Athens, GA 30602, USA.
authors.forename: Ralph A
authors.initials: RA
authors.lastname: Tripp
authors.affiliation: Emory University Department of Pediatrics and Children׳s Healthcare of Atlanta, Atlanta, GA 30322, USA.
authors.forename: Martin L
authors.initials: ML
authors.lastname: Moore
authors.affiliation: Emory University Department of Pediatrics and Children׳s Healthcare of Atlanta, Atlanta, GA 30322, USA. Electronic address: larry.anderson@emory.edu.
authors.forename: Larry J
authors.initials: LJ
authors.lastname: Anderson
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D000914:Antibodies, Viral / Q000627:therapeutic use*; D004195:Disease Models, Animal; D005260:Female; D007167:Immunotherapy / Q000379:methods; D008807:Mice, Inbred BALB C; D018357:Respiratory Syncytial Virus Infections / Q000628:therapy*; D016896:Treatment Outcome; D014759:Viral Envelope Proteins / Q000037:antagonists & inhibitors*; D014760:Viral Fusion Proteins / Q000037:antagonists & inhibitors*
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; C526850:F protein, human respiratory syncytial virus; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; C116204:attachment protein G
keywords: Anti-viral; RSV; RSV F protein; RSV G protein