title: Reduced efficiency of sarcolipin-dependent respiration in myocytes from humans with severe obesity.
abstract: OBJECTIVE
  Sarcolipin (SLN) regulates muscle energy expenditure through its action on sarco/endoplasmic reticulum Ca(2+) -ATPase (SERCA) pump. It is unknown whether SLN-dependent respiration has relevance to human obesity, but whole-transcriptome gene expression profiling revealed that SLN was more highly expressed in myocytes from individuals with severe obesity (OB) than in lean controls (LN). The purpose of this study was to examine SLN-dependent cellular respiratory rates in LN and OB human muscles.
  
  
  METHODS
  Primary myocytes were isolated from muscle biopsy from seven LN and OB Caucasian females. Cellular respiration was assessed with and without lentivirus-mediated SLN knockdown in LN and OB myocytes.
  
  
  RESULTS
  SLN mRNA and protein abundance was greater in OB compared to LN cells. Despite elevated SLN levels in wild-type OB cells, respiratory rates among SLN-deficient cells were higher in OB compared to LN. Obesity-induced reduction in efficiency of SLN-dependent respiration was associated with altered sarcoplasmic reticulum phospholipidome.
  
  
  CONCLUSIONS
  SLN-dependent respiration is reduced in muscles from humans with severe obesity compared to lean controls. Identification of the molecular mechanism that affects SLN efficiency might lead to interventions that promote an increase in skeletal muscle energy expenditure.
authors.affiliation: East Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, North Carolina, USA.|Department of Kinesiology, East Carolina University, Greenville, North Carolina, USA.|Department of Physiology, East Carolina University, Greenville, North Carolina, USA.
authors.forename: Christopher W
authors.initials: CW
authors.lastname: Paran
authors.affiliation: East Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, North Carolina, USA.|Department of Kinesiology, East Carolina University, Greenville, North Carolina, USA.
authors.forename: Anthony R P
authors.initials: AR
authors.lastname: Verkerke
authors.affiliation: East Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, North Carolina, USA.|Department of Kinesiology, East Carolina University, Greenville, North Carolina, USA.
authors.forename: Timothy D
authors.initials: TD
authors.lastname: Heden
authors.affiliation: East Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, North Carolina, USA.|Department of Kinesiology, East Carolina University, Greenville, North Carolina, USA.
authors.forename: Sanghee
authors.initials: S
authors.lastname: Park
authors.affiliation: East Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, North Carolina, USA.|Department of Kinesiology, East Carolina University, Greenville, North Carolina, USA.
authors.forename: Kai
authors.initials: K
authors.lastname: Zou
authors.affiliation: Department of Genetics, Washington University School of Medicine, St. Louis, Missouri, USA.
authors.forename: Heather A
authors.initials: HA
authors.lastname: Lawson
authors.affiliation: Medicine Mass Spectrometry Facility, Washington University School of Medicine, St. Louis, Missouri, USA.
authors.forename: Haowei
authors.initials: H
authors.lastname: Song
authors.affiliation: Medicine Mass Spectrometry Facility, Washington University School of Medicine, St. Louis, Missouri, USA.
authors.forename: John
authors.initials: J
authors.lastname: Turk
authors.affiliation: East Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, North Carolina, USA.|Department of Kinesiology, East Carolina University, Greenville, North Carolina, USA.
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Houmard
authors.affiliation: East Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, North Carolina, USA.|Department of Kinesiology, East Carolina University, Greenville, North Carolina, USA.|Department of Physiology, East Carolina University, Greenville, North Carolina, USA.
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Funai
mesh_terms: D004734:Energy Metabolism / Q000502:physiology; D005260:Female; D006801:Humans; D032342:Muscle Cells / Q000378:metabolism; D009124:Muscle Proteins / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism*; D009767:Obesity, Morbid / Q000378:metabolism*; D011510:Proteolipids / Q000378:metabolism*; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000378:metabolism*
chemical_list: D009124:Muscle Proteins; D011510:Proteolipids; C077258:sarcolipin; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases