title: Plasma Lactate Levels Increase during Hyperinsulinemic Euglycemic Clamp and Oral Glucose Tolerance Test.
abstract: Insulin resistance, which plays a central role in the pathogenesis of type 2 diabetes (T2D), is an early indicator that heralds the occurrence of T2D. It is imperative to understand the metabolic changes that occur at the cellular level in the early stages of insulin resistance. The objective of this study was to determine the pattern of circulating lactate levels during oral glucose tolerance test (OGTT) and hyperinsulinemic euglycemic clamp (HIEC) study in normal nondiabetic subjects. Lactate and glycerol were determined every 30 minutes during OGTT and HIEC on 22 participants. Lactate progressively increased throughout the HIEC study period (P < 0.001). Participants with BMI < 30 had significantly higher mean M-values compared to those with BMI ≥ 30 at baseline (P < 0.05). This trend also continued throughout the OGTT. In addition, those with impaired glucose tolerance test (IGT) had significantly higher mean lactate levels compared to those with normal glucose tolerance (P < 0.001). In conclusion, we found that lactate increased during HIEC study, which is a state of hyperinsulinemia similar to the metabolic milieu seen during the early stages in the development of T2D.
authors.affiliation: Wayne State University School of Medicine, Detroit, MI 48201, USA.
authors.forename: Feven
authors.initials: F
authors.lastname: Berhane
authors.affiliation: Wayne State University School of Medicine, Detroit, MI 48201, USA.
authors.forename: Alemu
authors.initials: A
authors.lastname: Fite
authors.affiliation: Wayne State University School of Medicine, Detroit, MI 48201, USA.
authors.forename: Nour
authors.initials: N
authors.lastname: Daboul
authors.affiliation: Wayne State University School of Medicine, Detroit, MI 48201, USA.
authors.forename: Wissam
authors.initials: W
authors.lastname: Al-Janabi
authors.affiliation: Wayne State University School of Medicine, Detroit, MI 48201, USA.
authors.forename: Zaher
authors.initials: Z
authors.lastname: Msallaty
authors.affiliation: Wayne State University School of Medicine, Detroit, MI 48201, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Caruso
authors.affiliation: Wayne State University School of Medicine, Detroit, MI 48201, USA.
authors.forename: Monique K
authors.initials: MK
authors.lastname: Lewis
authors.affiliation: Wayne State University School of Medicine, Detroit, MI 48201, USA.
authors.forename: Zhengping
authors.initials: Z
authors.lastname: Yi
authors.affiliation: Georgia Regents University, Augusta, GA 30912, USA.
authors.forename: Michael P
authors.initials: MP
authors.lastname: Diamond
authors.affiliation: Hamad Medical Corporation, Doha, Qatar.
authors.forename: Abdul-Badi
authors.initials: AB
authors.lastname: Abou-Samra
authors.affiliation: Wayne State University School of Medicine, Detroit, MI 48201, USA.
authors.forename: Berhane
authors.initials: B
authors.lastname: Seyoum
mesh_terms: D000328:Adult; D001786:Blood Glucose / Q000378:metabolism*; D015992:Body Mass Index; D005260:Female; D015309:Glucose Clamp Technique; D005951:Glucose Tolerance Test; D005990:Glycerol / Q000097:blood; D006801:Humans; D007328:Insulin / Q000097:blood*; D007333:Insulin Resistance; D019344:Lactic Acid / Q000097:blood*; D008055:Lipids / Q000097:blood; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000097:blood*
chemical_list: D001786:Blood Glucose; D007328:Insulin; D008055:Lipids; D019344:Lactic Acid; D005990:Glycerol