title: A Novel Label-Free microRNA-155 Detection on the Basis of Fluorescent Silver Nanoclusters.
abstract: In this paper, a new approach for microRNA-155 (miRNA-155) detection was described based on the fluorescence quenching of oligonucleotide-templated silver nanoclusters (DNA-AgNCs). The specific DNA scaffold with two different nucleotides fragments were used: one was enriched with a cytosine sequence fragment (C12) that could result in DNA-AgNCs with a high quantum yield via a chemical reduction method, and the other was the probe fragment (5- CUGUUAAUGCUAAUCGUG-3) which could selectively bind to the miRNA-155. Thus, the as-prepared AgNCs could exhibit quenched fluorescence when binding to the target miRNA-155. The fluorescence ratio of the DNA-AgNCs was quenched in a linearly proportional manner to the concentration of the target in the range of 0.2 nM to 30 nM with a detection limit of 0.1 nM.
authors.affiliation: Department of Life Science Engineering, Faculty of New Sciences & Technologies, University of Tehran, Tehran, Iran, smhosseini@khayam.ut.ac.ir.
authors.forename: Morteza
authors.initials: M
authors.lastname: Hosseini
authors.forename: Azam
authors.initials: A
authors.lastname: Akbari
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Ganjali
authors.forename: Mehdi
authors.initials: M
authors.lastname: Dadmehr
authors.forename: Ali Hossein
authors.initials: AH
authors.lastname: Rezayan
mesh_terms: D004396:Coloring Agents / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D005453:Fluorescence; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D035683:MicroRNAs / Q000032:analysis*; D012834:Silver / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D004396:Coloring Agents; C524942:MIRN155 microRNA, human; D035683:MicroRNAs; D012834:Silver; D004247:DNA