title: Variation of prostaglandin E2 concentrations in ovaries and its effects on ovarian maturation and oocyte proliferation in the giant fresh water prawn, Macrobrachium rosenbergii.
abstract: Prostaglandins (PGs) are important bioactive mediators for many physiological functions. In some decapod crustaceans, prostaglandin E2 (PGE2) has been detected in reproductive organs, and may play a role in the control of ovarian maturation. However, in the freshwater prawn, Macrobrachium rosenbergii, the presences of PGE2 and key enzymes for PGE2 biosynthesis, as well as its effects on ovarian maturation have not yet been investigated. In this study we reported the presence of PGE2, cyclooxygenase1 (COX1) and prostaglandin E synthase (PGES) in the ovarian tissues of M. rosenbergii, using immunohistochemistry. Intense immunoreactivities of PGE2 (PGE2-ir), COX1 (Cox1-ir) and PGES (PGES-ir) were detected in previtellogenic oocytes (Oc1 and Oc2), while the immunoreactivities were absent in the late vitellogenic oocytes (Oc4). This finding supports the hypothesis that the PGE2 biosynthesis occurs in the ovary of this prawn. To ascertain this finding we used LC-MS/MS to quantitate PGE2 concentrations during ovarian developmental cycle. The levels of PGE2 were significantly higher in the early ovarian stages (St I and II) than in the late stages (St III and IV). Moreover, we found that administration of PGE2 stimulated the ovarian maturation in this species by shortening the length of the ovarian cycle, increasing ovarian-somatic index, oocyte proliferation, and vitellogenin (Vg) level in the hemolymph.
authors.affiliation: Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Road, Ratchathewi, Bangkok 10400, Thailand. Electronic address: chanudpornsumpownon@gmail.com.
authors.forename: Chanudporn
authors.initials: C
authors.lastname: Sumpownon
authors.affiliation: Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Road, Ratchathewi, Bangkok 10400, Thailand.
authors.forename: Attakorn
authors.initials: A
authors.lastname: Engsusophon
authors.affiliation: Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Road, Ratchathewi, Bangkok 10400, Thailand.
authors.forename: Tanapan
authors.initials: T
authors.lastname: Siangcham
authors.affiliation: Department of Cell Biology and Anatomy, School of Medicine, Hamamatsu University, Hamamatsu, Shizuoka, Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Sugiyama
authors.affiliation: Department of Preclinical Science, Faculty of Medicine, Thammasat University, Pathumthani 12120, Thailand.
authors.forename: Nantawan
authors.initials: N
authors.lastname: Soonklang
authors.affiliation: Deparment of Biomedical Sciences, Faculty of Allied Health Sciences, Burapha University, Chonburi 20131, Thailand.
authors.forename: Prasert
authors.initials: P
authors.lastname: Meeratana
authors.affiliation: Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Road, Ratchathewi, Bangkok 10400, Thailand.
authors.forename: Chaitip
authors.initials: C
authors.lastname: Wanichanon
authors.affiliation: Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Road, Ratchathewi, Bangkok 10400, Thailand; Pro Vice-Chancellor's Office, Faculty of Science and Technology, Deakin University, Locked Bag 2000, Geelong, Victoria 3220, Australia.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Hanna
authors.affiliation: Department of Cell Biology and Anatomy, School of Medicine, Hamamatsu University, Hamamatsu, Shizuoka, Japan.
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Setou
authors.affiliation: Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Road, Ratchathewi, Bangkok 10400, Thailand. Electronic address: prasert.sob@mahidol.ac.th.
authors.forename: Prasert
authors.initials: P
authors.lastname: Sobhon
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D051545:Cyclooxygenase 1 / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism*; D005260:Female; D005618:Fresh Water; D006458:Hemolymph / Q000378:metabolism; D007150:Immunohistochemistry; D019746:Intramolecular Oxidoreductases / Q000378:metabolism; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism; D010053:Ovary / Q000166:cytology* / Q000378:metabolism*; D033541:Palaemonidae / Q000254:growth & development* / Q000378:metabolism*; D000071876:Prostaglandin-E Synthases; D053719:Tandem Mass Spectrometry; D014819:Vitellogenins / Q000378:metabolism*
chemical_list: D014819:Vitellogenins; D051545:Cyclooxygenase 1; D019746:Intramolecular Oxidoreductases; D000071876:Prostaglandin-E Synthases; D015232:Dinoprostone
keywords: Cyclooxygenase1; In vivo bioassay; Macrobrachium rosenbergii; Ovarian maturation; Prostaglandin E synthase; Prostaglandin E(2)