title: Determination of pyruvic acid concentration using a bioluminescence system from Photobacterium leiognathi.
abstract: A novel, highly sensitive and selective bacterial luminescence method for the detection of pyruvic acid (PA) is reported here. This method is based on a reaction system catalyzed by lactate dehydrogenase (LDH) with the bacterial luciferase-FMN:NADH oxidoreductase bioluminescence system in vitro. The reduced nicotinamide adenine dinucleotide (NADH) involved in the LDH reaction system could be quantitatively analyzed by the bioluminescence system. A good linear relationship between the luminescence intensity and pyruvic acid concentration was exhibited within the range of 0.00014-0.001 mol l(-1), and the pyruvic acid detection limit was found to be 8.537 × 10(-5) mol l(-1). This method was successfully applied to the detection of PA in quail serum with a good recovery of over 70%.
authors.affiliation: Food Safety Laboratory, College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China. snow@ouc.edu.cn.
authors.forename: Guanhua
authors.initials: G
authors.lastname: Xuan
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Lu
authors.forename: Jingxue
authors.initials: J
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Lin
authors.forename: Huihui
authors.initials: H
authors.lastname: Liu
mesh_terms: D057230:Limit of Detection; D008156:Luciferases / Q000737:chemistry; D008163:Luminescent Measurements / Q000379:methods*; D010776:Photobacterium / Q000201:enzymology; D019289:Pyruvic Acid / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D019289:Pyruvic Acid; D008156:Luciferases