title: Assessment of genotoxic potential of hridayarnava rasa (a herbo-mineralo-metallic ayurvedic formulation) using chromosomal aberration and sperm abnormality assays.
abstract: OBJECTIVE
  Herbo-mineral formulations are being successfully used in therapeutics since centuries. But recently, they came under the scanner for their metallic contents especially the presence of heavy metals. Hence it is the need of the hour to assess and establish the safety of these formulations through toxicity studies. In line with the various toxicity studies that are being carried out, Government of India expressed the need for conducting genotoxicity studies of different metal- or mineral-based drugs. Till date very few Ayurvedic herbo-mineral formulations have been studied for their genotoxic potential. The present study is aimed to evaluate the genotoxic potential of Hridayarnava Rasa.
  
  
  METHODS
  It was prepared as per classical guidelines and administered to Swiss albino mice for 14 consecutive days. Chromosomal aberration and sperm abnormality assay were done to evaluate the genotoxic potential of the test drugs. Cyclophosphamide (CP) was taken as positive group and results were compared.
  
  
  RESULTS
  All treated groups exhibited significant body weight gain in comparison to CP group. Results revealed no structural deformity in the above parameters in comparison to the CP-treated group.
  
  
  CONCLUSIONS
  Reported data showed that both tested samples of Hridayarnava Rasa does not possess genotoxic potential under the experimental conditions and can be safely used.
authors.affiliation: Department of Rasashastra and Bhaishajya Kalpana, Parul Institute of Ayurveda, Limda, Vadodara, India.
authors.forename: Chandrashekhar Y
authors.initials: CY
authors.lastname: Jagtap
authors.affiliation: Department of Rasashastra and Bhaishajya Kalpana, Institute of Post Graduate Teaching and Research in Ayurveda, Gujarat Ayurved University, Jamnagar, Gujarat, India.
authors.forename: Swapnil Y
authors.initials: SY
authors.lastname: Chaudhari
authors.affiliation: Department of Pharmacology, Swargiy Sanjibhai Roopajibhai College of Pharmacy, Silvassa, Union Territory of Dadra and Nagar Haveli.
authors.forename: Jalaram H
authors.initials: JH
authors.lastname: Thakkar
authors.affiliation: Department of Rasashastra and Bhaishajya Kalpana, Institute of Post Graduate Teaching and Research in Ayurveda, Gujarat Ayurved University, Jamnagar, Gujarat, India.
authors.forename: R
authors.initials: R
authors.lastname: Galib
authors.affiliation: Department of Rasashastra and Bhaishajya Kalpana, Institute of Post Graduate Teaching and Research in Ayurveda, Gujarat Ayurved University, Jamnagar, Gujarat, India.
authors.forename: P K
authors.initials: PK
authors.lastname: Prajapati
keywords: Colchicine; Tamra bhasma; cyclophosphamide; genotoxicity; herbo-mineral formulations