title: Correlation between polymorphism of platelet alloantigen genes HPA-1-5 and type 2 diabetes complication by carotid atherosclerosis in a Chinese population.
abstract: We investigated the association between the polymorphism of human platelet alloantigen genes HPA-1-HPA-5 and the complication of type 2 diabetes mellitus (T2DM) by carotid atherosclerosis (CA) among Han people in Guiyang District, China. Ninety-nine T2DM patients were selected from the Affiliated Hospital of Guiyang Medical College and divided into a CA(+) group and a CA(-) group. A control group comprised 100 healthy people from the medical examination center of the same hospital. Genomic DNA from all the subjects was isolated by phenol-chloroform extraction and target genes were amplified using sequence-specific primer-polymerase chain reaction, followed by gene type detection of HPA. There were significant differences in allele and genotype frequencies of HPA-1, -2, -3, and -5 among the three groups [CA(+), CA(-), and the control group] (P < 0.05), and significant differences in allele and genotype frequencies of HPA-1, -2, and -3 between groups CA(+) and CA(-) and the control group (P < 0.05). Moreover, there was a significant difference in allele and genotype frequencies of HPA-5 between the CA(+) and CA(-) groups (P < 0.05). Logistic regression analysis showed that risk factors for T2DM patients developing a CA complication were age, duration of diabetes, high blood pressure, smoking, overweight, abnormal blood lipid levels, and polymorphism of HPA-5. There may be a correlation between T2DM and polymorphism of HPA-1-3. Polymorphism of HPA- 5 is probably a risk factor for CA complicating T2DM.
authors.affiliation: Emergency Department, Affiliated Hospital of Guiyang Medical College, Guiyang, Guizhou, China.
authors.forename: Y H
authors.initials: YH
authors.lastname: Zhang
authors.affiliation: Intensive Care Unit, Wenzhou People's Hospital, Wenzhou, Zhejiang, China.
authors.forename: S F
authors.initials: SF
authors.lastname: Xu
authors.affiliation: Endocrinology Department, Affiliated Hospital of Guiyang Medical College, Guiyang, Guizhou, China.
authors.forename: J
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Emergency Department, Affiliated Hospital of Guiyang Medical College, Guiyang, Guizhou, China.
authors.forename: H S
authors.initials: HS
authors.lastname: Hong
authors.affiliation: Emergency Department, Affiliated Hospital of Guiyang Medical College, Guiyang, Guizhou, China Fanliangmin_flm@163.com.
authors.forename: L M
authors.initials: LM
authors.lastname: Fan
mesh_terms: D000368:Aged; D016824:Antigens, Human Platelet / Q000235:genetics*; D002340:Carotid Artery Diseases / Q000209:etiology / Q000235:genetics*; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000235:genetics*; D003925:Diabetic Angiopathies / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide
chemical_list: D016824:Antigens, Human Platelet