title: In Vivo Kinetics of Mesenchymal Stem Cells Transplanted into the Knee Joint in a Rat Model Using a Novel Magnetic Method of Localization.
abstract: We have developed a magnetic system for targeting cells in minimally invasive cell transplantation. Magnetically labeled MSCs (m-MSCs) with nanoscale iron particles can be guided into the desired region by magnetic force from an extracorporeal device. We reported that magnetic targeting of m-MSCs enhances cartilage repair in a mini-pig model. However, the detailed kinetics of these magnetically targeted m-MSCs remain unknown. For clinical use, this aspect should be clarified from a safety standpoint. We therefore investigated the spatial and temporal distribution of the fluorescently-labeled m-MSCs transplanted into the knee joint using in vivo fluorescence combined with three-dimensional computed tomographic imaging in a rat model. Although the intraarticularly injected m-MSCs were spread throughout the joint cavity in the absence of magnetic force, the magnetic force caused the injected m-MSCs to accumulate around the chondral lesion. Further examinations including ex vivo imaging, histological assessments and reverse transcription polymerase chain reaction revealed that transplanted MSCs were not present in any major organs after intraarticular administration, regardless of magnetic targeting. Our data suggest that m-MSCs can be accumulated efficiently into a chondral lesion using our magnetic targeting system, while none of the intraarticularly transplanted MSCs migrate to other major organs.
authors.affiliation: Department of Orthopedic Surgery, Graduate School of Biomedical and Health Science, Hiroshima University, Hiroshima, Japan.
authors.forename: Yasunari
authors.initials: Y
authors.lastname: Ikuta
authors.affiliation: Department of Orthopedic Surgery, Graduate School of Biomedical and Health Science, Hiroshima University, Hiroshima, Japan.
authors.forename: Naosuke
authors.initials: N
authors.lastname: Kamei
authors.affiliation: Department of Orthopedic Surgery, Graduate School of Biomedical and Health Science, Hiroshima University, Hiroshima, Japan.
authors.forename: Masakazu
authors.initials: M
authors.lastname: Ishikawa
authors.affiliation: Department of Orthopedic Surgery, Graduate School of Biomedical and Health Science, Hiroshima University, Hiroshima, Japan.
authors.forename: Nobuo
authors.initials: N
authors.lastname: Adachi
authors.affiliation: Department of Orthopedic Surgery, Graduate School of Biomedical and Health Science, Hiroshima University, Hiroshima, Japan.
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Ochi
mesh_terms: D000818:Animals; D002232:Carbocyanines / Q000378:metabolism; D002465:Cell Movement; D058948:Cell Tracking / Q000379:methods*; D002478:Cells, Cultured; D003287:Contrast Media / Q000378:metabolism; D003911:Dextrans / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes / Q000378:metabolism; D005786:Gene Expression Regulation; D006614:Hindlimb; D006801:Humans; D007700:Kinetics; D007719:Knee Joint / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D008280:Magnetics; D058185:Magnetite Nanoparticles; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D023421:Models, Animal; D061848:Optical Imaging; D011916:Rats, Inbred F344; D011923:Rats, Nude; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D002232:Carbocyanines; D003287:Contrast Media; D003911:Dextrans; D005456:Fluorescent Dyes; D058185:Magnetite Nanoparticles; C065507:ferumoxides
keywords: marrow/stem cell transplantation; stem cell transplantation; surgery