title: [Effect of YM155 on Apoptosis and Autophagy of K562 Cells].
abstract: OBJECTIVE
  This study was purposed to investigate the effect of YM155, a survivin inhibitor, on the apoptosis and autophagy of K562 cells.
  
  
  METHODS
  K562 cells were treated with YM155 at different concentration. Cell survival was analyzed by CCK-8 assay, the cell apoptosis was detected by flow cytometry. Survivin, BCL-2 and beclin1 mRNA expressions were determined by RT-PCR. Survivin, BCL-2, caspase-3, PARP and LC-3 protein expressions were assayed by Western blot.
  
  
  RESULTS
  YM155 inhibited the proliferation of K562 cells in a time- and dose-dependent manners. With the increasing of YM155 concentration and prolonging of action time, the expression levels of mRNA and protein of survivin and BCL-2 decreased, while the expression levels of caspase-3, PARP, beclin1 and LC-3 increased. Compared with the YM155 group, the protein levels of LC-3 and caspase-3 were lower in YM155 combined with 3-MA group.
  
  
  CONCLUSIONS
  YM155 can inhibit K562 cell proliferation by inducing apoptosis and autophagy, while autophagy induction effect can enhance its cytotoxic effect.
authors.affiliation: Department of Hematology, Huai'an First People's Hospital of Nanjing Medical University, Huai'an, 223300, Jiangsu province, China.
authors.forename: Yi-Han
authors.initials: YH
authors.lastname: Ding
authors.affiliation: Department of Hematology, Huai'an First People's Hospital of Nanjing Medical University, Huai'an, 223300, Jiangsu province, China.
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Fan
authors.affiliation: Department of Hematology, Huai'an First People's Hospital of Nanjing Medical University, Huai'an, 223300, Jiangsu province, China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Wu
authors.affiliation: Department of Hematology, Huai'an First People's Hospital of Nanjing Medical University, Huai'an, 223300, Jiangsu province, China.
authors.forename: Zhi-Kui
authors.initials: ZK
authors.lastname: Deng
authors.affiliation: Department of Hematology, Huai'an First People's Hospital of Nanjing Medical University, Huai'an, 223300, Jiangsu province, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wei
authors.affiliation: Department of Hematology, Huai'an First People's Hospital of Nanjing Medical University, Huai'an, 223300, Jiangsu province, China. E-mail: liyufeng99@netease.com.
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Li
mesh_terms: D017209:Apoptosis; D001343:Autophagy; D049109:Cell Proliferation; D005434:Flow Cytometry; D006801:Humans; D007093:Imidazoles; D020014:K562 Cells; D009285:Naphthoquinones
chemical_list: D007093:Imidazoles; D009285:Naphthoquinones; C523798:sepantronium