title: Urine Collection Method for the Diagnosis of Congenital Cytomegalovirus Infection.
abstract: Congenital cytomegalovirus infection is traditionally diagnosed by virus detection in saliva or urine. Virus culture was positive in significantly fewer urine samples collected using cotton balls in diapers (55.2%) than with samples collected by bags (93.2%) from newborns screened positive for CMV in saliva. However, polymerase chain reaction was positive in 95% of urine samples regardless of the collection method.
authors.affiliation: From the *Department of Pediatrics, †Department of Microbiology, The University of Alabama at Birmingham, Birmingham, Alabama; ‡Department of Pediatrics, Carolinas Medical Center, Charlotte, North Carolina; §Department of Pediatrics, University of Mississippi Medical Center, Jackson, Mississippi; ¶Department of Pediatrics, The Children's Hospital of Pittsburgh of UPMC, Pittsburgh, Pennsylvania; ‖Department of Pediatrics, The Ohio State University-Nationwide Children's Hospital, Columbus, Ohio; **Department of Pediatrics, University of Texas Southwestern Medical School, Dallas, Texas; ††Department of Pediatrics, Cincinnati Children's Hospital Medical Center and University of Cincinnati, Cincinnati, Ohio; ‡‡Saint Peter's University Hospital, New Brunswick, New Jersey; and §§Department of Epidemiology, The University of Alabama at Birmingham, Birmingham, Alabama.
authors.forename: Shannon A
authors.initials: SA
authors.lastname: Ross
authors.forename: Amina
authors.initials: A
authors.lastname: Ahmed
authors.forename: April L
authors.initials: AL
authors.lastname: Palmer
authors.forename: Marian G
authors.initials: MG
authors.lastname: Michaels
authors.forename: Pablo J
authors.initials: PJ
authors.lastname: Sánchez
authors.forename: Audra
authors.initials: A
authors.lastname: Stewart
authors.forename: David I
authors.initials: DI
authors.lastname: Bernstein
authors.forename: Kristina
authors.initials: K
authors.lastname: Feja
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Novak
authors.forename: Karen B
authors.initials: KB
authors.lastname: Fowler
authors.forename: Suresh B
authors.initials: SB
authors.lastname: Boppana
mesh_terms: D003587:Cytomegalovirus / Q000235:genetics; D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D016133:Polymerase Chain Reaction; D059349:Urine Specimen Collection / Q000379:methods* / Q000592:standards; D014773:Virology