title: Myoblast cytonemes mediate Wg signaling from the wing imaginal disc and Delta-Notch signaling to the air sac primordium.
abstract: The flight muscles, dorsal air sacs, wing blades, and thoracic cuticle of the Drosophila adult function in concert, and their progenitor cells develop together in the wing imaginal disc. The wing disc orchestrates dorsal air sac development by producing decapentaplegic and fibroblast growth factor that travel via specific cytonemes in order to signal to the air sac primordium (ASP). Here, we report that cytonemes also link flight muscle progenitors (myoblasts) to disc cells and to the ASP, enabling myoblasts to relay signaling between the disc and the ASP. Frizzled (Fz)-containing myoblast cytonemes take up Wingless (Wg) from the disc, and Delta (Dl)-containing myoblast cytonemes contribute to Notch activation in the ASP. Wg signaling negatively regulates Dl expression in the myoblasts. These results reveal an essential role for cytonemes in Wg and Notch signaling and for a signal relay system in the myoblasts.
authors.affiliation: Cardiovascular Research Institute, University of California, San Francisco, San Francisco, United States.
authors.forename: Hai
authors.initials: H
authors.lastname: Huang
authors.affiliation: Cardiovascular Research Institute, University of California, San Francisco, San Francisco, United States.
authors.forename: Thomas B
authors.initials: TB
authors.lastname: Kornberg
mesh_terms: D000400:Air Sacs / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D000818:Animals; D019521:Body Patterning / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D004625:Embryo, Nonmammalian; D005346:Fibroblast Growth Factors / Q000235:genetics / Q000378:metabolism; D051157:Frizzled Receptors / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D060227:Imaginal Discs / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism; D007814:Larva / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D032446:Myoblasts / Q000166:cytology / Q000378:metabolism*; D021381:Protein Transport; D051880:Receptors, Notch / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D014921:Wings, Animal / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D051155:Wnt1 Protein / Q000235:genetics* / Q000378:metabolism; D000097573:Red Fluorescent Protein
chemical_list: D029721:Drosophila Proteins; D005346:Fibroblast Growth Factors; D051157:Frizzled Receptors; D049452:Green Fluorescent Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008164:Luminescent Proteins; D008565:Membrane Proteins; D051880:Receptors, Notch; D051155:Wnt1 Protein; D000097573:Red Fluorescent Protein; C497759:N protein, Drosophila; C064045:delta protein; C064196:dpp protein, Drosophila; C058784:fz protein, Drosophila; C074622:wg protein, Drosophila
keywords: D. melanogaster; Delta; Notch; Wingless; cell biology; cytoneme; developmental biology; diaphanous; frizzled; stem cells