title: Surface modification of MPEG-b-PCL-based nanoparticles via oxidative self-polymerization of dopamine for malignant melanoma therapy.
abstract: To enhance the therapeutic effects of chemotherapy on malignant melanoma, paclitaxel (PTX)-loaded methoxy poly(ethylene glycol)-b-poly(ε-caprolactone) nanoparticles (MPEG-b-PCL NPs) that had their surfaces modified with polydopamine (PTX-loaded MPEG-b-PCL NPs@PDA) were prepared as drug vehicles. The block copolymer MPEG-b-PCL was synthesized by ring-opening polymerization and characterized by proton nuclear magnetic resonance spectroscopy and gel permeation chromatography. The PTX-loaded NPs were prepared by a modified nanoprecipitation technique. The PTX-loaded NPs and PTX-loaded NPs@PDA were characterized in terms of size and size distribution, zeta potential, surface morphology, drug encapsulation efficiency, and drug release. Confocal laser scanning microscopy showed that coumarin-6-loaded NPs@PDA could be internalized by human melanoma cell line A875 cells. The cellular uptake efficiency of NPs was greatly enhanced after PDA modification. The antitumor efficacy of the PTX-loaded NPs@PDA was investigated in vitro by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and in vivo by a xenograft tumor model. The PTX-loaded NPs@PDA could significantly inhibit tumor growth compared to Taxol(®) and precursor PTX-loaded NPs. All the results suggested that the PTX-loaded MPEG-b-PCL NPs that had their surfaces modified with PDA are promising nanocarriers for malignant melanoma therapy.
authors.affiliation: Southern Medical University, Guangzhou, People's Republic of China ; Department of Plastic Surgery, General Hospital of Guangzhou Military Command of PLA, Guangzhou, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xiong
authors.affiliation: Southern Medical University, Guangzhou, People's Republic of China ; Department of Plastic Surgery, General Hospital of Guangzhou Military Command of PLA, Guangzhou, People's Republic of China.
authors.forename: Lixia
authors.initials: L
authors.lastname: Peng
authors.affiliation: Division of Life Sciences and Health, Graduate School at Shenzhen, Tsinghua University, Shenzhen, People's Republic of China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Chen
authors.affiliation: Southern Medical University, Guangzhou, People's Republic of China ; Department of Plastic Surgery, General Hospital of Guangzhou Military Command of PLA, Guangzhou, People's Republic of China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Li
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D007211:Indoles / Q000737:chemistry*; D008545:Melanoma; D053758:Nanoparticles / Q000737:chemistry*; D017239:Paclitaxel / Q000737:chemistry / Q000494:pharmacology; D011091:Polyesters / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D000970:Antineoplastic Agents; D007211:Indoles; D011091:Polyesters; D011108:Polymers; C439611:methoxy poly(ethylene glycol-co-epsilon-caprolactone); C568283:polydopamine; D011092:Polyethylene Glycols; D017239:Paclitaxel
keywords: cancer nanotechnology; drug delivery; malignant melanoma; polydopamine; surface modification