title: A multivariate approach for high throughput pectin profiling by combining glycan microarrays with monoclonal antibodies.
abstract: Pectin-one of the most complex biomacromolecules in nature has been extensively studied using various techniques. This has been done so in an attempt to understand the chemical composition and conformation of pectin, whilst discovering and optimising new industrial applications of the polymer. For the last decade the emergence of glycan microarray technology has led to a growing capacity of acquiring simultaneous measurements related to various carbohydrate characteristics while generating large collections of data. Here we used a multivariate analysis approach in order to analyse a set of 359 pectin samples probed with 14 different monoclonal antibodies (mAbs). Principal component analysis (PCA) and partial least squares (PLS) regression were utilised to obtain the most optimal qualitative and quantitative information from the spotted microarrays. The potential use of microarray technology combined with chemometrics for the accurate determination of degree of methyl-esterification (DM) and degree of blockiness (DB) was assessed.
authors.affiliation: University of Copenhagen, Faculty of Science, Department of Plant and Environmental Sciences, DK-1871 Frederiksberg, Denmark; CP Kelco ApS., Ved Banen 16, DK-4623 Lille Skensved, Denmark.
authors.forename: António G
authors.initials: AG
authors.lastname: Sousa
authors.affiliation: University of Copenhagen, Faculty of Science, Department of Plant and Environmental Sciences, DK-1871 Frederiksberg, Denmark.
authors.forename: Louise I
authors.initials: LI
authors.lastname: Ahl
authors.affiliation: University of Copenhagen, Faculty of Science, Department of Plant and Environmental Sciences, DK-1871 Frederiksberg, Denmark.
authors.forename: Henriette L
authors.initials: HL
authors.lastname: Pedersen
authors.affiliation: University of Copenhagen, Faculty of Science, Department of Plant and Environmental Sciences, DK-1871 Frederiksberg, Denmark.
authors.forename: Jonatan U
authors.initials: JU
authors.lastname: Fangel
authors.affiliation: CP Kelco ApS., Ved Banen 16, DK-4623 Lille Skensved, Denmark. Electronic address: susanne.o.soerensen@cpkelco.com.
authors.forename: Susanne O
authors.initials: SO
authors.lastname: Sørensen
authors.affiliation: University of Copenhagen, Faculty of Science, Department of Plant and Environmental Sciences, DK-1871 Frederiksberg, Denmark.
authors.forename: William G T
authors.initials: WG
authors.lastname: Willats
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry*; D046228:Microarray Analysis / Q000379:methods*; D010368:Pectins / Q000032:analysis* / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*
chemical_list: D000911:Antibodies, Monoclonal; D011134:Polysaccharides; D010368:Pectins
keywords: Chemometrics; Esterification; Glycan microarrays; Monoclonal antibodies; Pectin