title: Immunohistochemical detection of IgM and IgG in lung tissue of dogs with leptospiral pulmonary haemorrhage syndrome (LPHS).
abstract: Leptospiral pulmonary haemorrhage syndrome (LPHS) is a severe form of leptospirosis. Pathogenic mechanisms are poorly understood. Lung tissues from 26 dogs with LPHS, 5 dogs with pulmonary haemorrhage due to other causes and 6 healthy lungs were labelled for IgG (n=26), IgM (n=25) and leptospiral antigens (n=26). Three general staining patterns for IgG/IgM were observed in lungs of dogs with LPHS with most tissues showing more than one staining pattern: (1) alveolar septal wall staining, (2) staining favouring alveolar surfaces and (3) staining of intra-alveolar fluid. Healthy control lung showed no staining, whereas haemorrhagic lung from dogs not infected with Leptospira showed staining of intra-alveolar fluid and occasionally alveolar septa. Leptospiral antigens were not detected. We conclude that deposition of IgG/IgM is demonstrable in the majority of canine lungs with naturally occurring LPHS, similar to what has been described in other species. Our findings suggest involvement of the host humoral immunity in the pathogenesis of LPHS and provide further evidence to support the dog as a natural disease model for human LPHS.
authors.affiliation: University College Dublin, School of Veterinary Medicine, Dublin, Ireland; Vetsuisse Faculty Bern, Länggassstrasse 128, 3012 Bern, Switzerland. Electronic address: simone.schuller@vetsuisse.unibe.ch.
authors.forename: Simone
authors.initials: S
authors.lastname: Schuller
authors.affiliation: University College Dublin, School of Veterinary Medicine, Dublin, Ireland; Ross University School of Veterinary Medicine, St Kitts, West Indies.
authors.forename: John J
authors.initials: JJ
authors.lastname: Callanan
authors.affiliation: University College Dublin, School of Veterinary Medicine, Dublin, Ireland.
authors.forename: Sheila
authors.initials: S
authors.lastname: Worrall
authors.affiliation: Vetsuisse Faculty Bern, Länggassstrasse 128, 3012 Bern, Switzerland.
authors.forename: Thierry
authors.initials: T
authors.lastname: Francey
authors.affiliation: Vetsuisse Faculty Bern, Länggassstrasse 128, 3012 Bern, Switzerland.
authors.forename: Ariane
authors.initials: A
authors.lastname: Schweighauser
authors.affiliation: FU Berlin, Robert-von-Ostertag-Str. 15, 14163 Berlin, Germany.
authors.forename: Barbara
authors.initials: B
authors.lastname: Kohn
authors.affiliation: FU Berlin, Robert-von-Ostertag-Str. 15, 14163 Berlin, Germany.
authors.forename: Robert
authors.initials: R
authors.lastname: Klopfleisch
authors.affiliation: Vetsuisse Faculty Bern, Länggassstrasse 128, 3012 Bern, Switzerland.
authors.forename: Horst
authors.initials: H
authors.lastname: Posthaus
authors.affiliation: University College Dublin, School of Veterinary Medicine, Dublin, Ireland.
authors.forename: Jarlath E
authors.initials: JE
authors.lastname: Nally
mesh_terms: D000818:Animals; D015551:Autoimmunity; D004195:Disease Models, Animal; D004285:Dogs; D006470:Hemorrhage / Q000276:immunology / Q000382:microbiology; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis* / Q000276:immunology; D007075:Immunoglobulin M / Q000032:analysis* / Q000276:immunology; D007150:Immunohistochemistry / Q000379:methods; D007919:Leptospira / Q000302:isolation & purification* / Q000472:pathogenicity; D007922:Leptospirosis / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D008168:Lung / Q000276:immunology* / Q000473:pathology; D008171:Lung Diseases / Q000276:immunology* / Q000382:microbiology; D013577:Syndrome
chemical_list: D007074:Immunoglobulin G; D007075:Immunoglobulin M
keywords: Dog; Leptospiral pulmonary haemorrhage syndrome; Leptospirosis