title: Hydrogen sulfide in posthemorrhagic shock mesenteric lymph drainage alleviates kidney injury in rats.
abstract: Posthemorrhagic shock mesenteric lymph (PHSML) is a key factor in multiple organ injury following hemorrhagic shock. We investigated the role of hydrogen sulfide (H2S) in PHSML drainage in alleviating acute kidney injury (AKI) by administering D,L-propargylglycine (PPG) and sodium hydrosulfide hydrate (NaHS) to 12 specific pathogen-free male Wistar rats with PHSML drainage. A hemorrhagic shock model was established in 4 experimental groups: shock, shock+drainage, shock+drainage+PPG (45 mg/kg, 0.5 h prehemorrhage), and shock+drainage+NaHS (28 µmol/kg, 0.5 h prehemorrhage). Fluid resuscitation was performed after 1 h of hypotension, and PHMSL was drained in the last three groups for 3 h after resuscitation. Renal function and histomorphology were assessed along with levels of H2S, cystathionine-γ-lyase (CSE), Toll-like receptor 4 (TLR4), interleukin (IL)-10, IL-12, and tumor necrosis factor (TNF)-α in renal tissue. Hemorrhagic shock induced AKI with increased urea and creatinine levels in plasma and higher H2S, CSE, TLR4, IL-10, IL-12, and TNF-α levels in renal tissue. PHSML drainage significantly reduced urea, creatinine, H2S, CSE, and TNF-α but not TLR4, IL-10, or IL-12. PPG decreased creatinine, H2S, IL-10, and TNF-α levels, but this effect was reversed by NaHS administration. In conclusion, PHSML drainage alleviated AKI following hemorrhagic shock by preventing increases in H2S and H2S-mediated inflammation.
authors.affiliation: Institute of Microcirculation, Hebei North University, Hebei Zhangjiakou, Brasil.
authors.forename: B
authors.initials: B
authors.lastname: Han
authors.affiliation: Institute of Microcirculation, Hebei North University, Hebei Zhangjiakou, Brasil.
authors.forename: Z G
authors.initials: ZG
authors.lastname: Zhao
authors.affiliation: Institute of Microcirculation, Hebei North University, Hebei Zhangjiakou, Brasil.
authors.forename: L M
authors.initials: LM
authors.lastname: Zhang
authors.affiliation: Institute of Microcirculation, Hebei North University, Hebei Zhangjiakou, Brasil.
authors.forename: S G
authors.initials: SG
authors.lastname: Li
authors.affiliation: Institute of Microcirculation, Hebei North University, Hebei Zhangjiakou, Brasil.
authors.forename: C Y
authors.initials: CY
authors.lastname: Niu
mesh_terms: D058186:Acute Kidney Injury / Q000503:physiopathology / Q000517:prevention & control*; D000480:Alkynes / Q000627:therapeutic use; D000818:Animals; D003404:Creatinine / Q000097:blood; D003542:Cystathionine gamma-Lyase / Q000032:analysis; D016207:Cytokines / Q000032:analysis; D004322:Drainage / Q000379:methods*; D004791:Enzyme Inhibitors / Q000627:therapeutic use; D004797:Enzyme-Linked Immunosorbent Assay; D064426:Gasotransmitters / Q000032:analysis / Q000627:therapeutic use*; D005998:Glycine / Q000031:analogs & derivatives / Q000627:therapeutic use; D006862:Hydrogen Sulfide / Q000032:analysis / Q000627:therapeutic use*; D008196:Lymph / Q000502:physiology*; D008297:Male; D008643:Mesentery; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012771:Shock, Hemorrhagic / Q000150:complications / Q000628:therapy*; D013447:Sulfites / Q000627:therapeutic use; D013997:Time Factors; D016896:Treatment Outcome; D014508:Urea / Q000097:blood
chemical_list: D000480:Alkynes; D016207:Cytokines; D004791:Enzyme Inhibitors; D064426:Gasotransmitters; D013447:Sulfites; C009055:propargylglycine; D014508:Urea; C569244:sodium hydrogen sulfite; D003404:Creatinine; D003542:Cystathionine gamma-Lyase; D005998:Glycine; D006862:Hydrogen Sulfide