title: Sandwich-format electrochemiluminescence assay for PDGF-BB using quantum dots-dendrimer nanocomposites as probe.
abstract: This work describes a novel electrochemiluminescence aptasensor for highly sensitive detection of platelet-derived growth factor BB (PDGF-BB) using aptamer functionalized CdS quantum dots-polyamidoamine as probe (CdS QDs-PAMAM-Apt). CdS QDs-PAMAM nanocomposites were synthesized by one-pot synthesis in methanol. The prepared nanocomposites were linked with the NH2-aptamer 2 (Apt2) of PDGF-BB to form the CdS QDs-PAMAM-Apt2 probe by glutaraldehyde as coupling reagent. For constructing the aptasensor, MWCNTs-chitosan composites and NH2-aptamer 1 (Apt1) with the same base sequence as Apt2 were immobilized on the electrode by the self-assembled method to recognize the target protein PDGF-BB. In the presence of PDGF-BB, the structure of sandwiched format was formed between the Apt1 and the CdS QDs-PAMAM-Apt2 probe, thereby resulting in a proportional increase of ECL emission. Thanks to the efficient and stable ECL emission of CdS QDs-PAMAM dendrimer and the advantage of MWCNTs for accelerating the electron transfer, the highly sensitive detection of PDGF-BB with a detection limit of 0.13pM was achieved. The linear range is from 0.5pM to 1nM. The present protocol was applied to the analysis of PDGF-BB in human serum samples. The recoveries of PDGF-BB in human serum samples are 87.2-113% and RSD values are less than 3.6%.
authors.affiliation: College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.
authors.forename: Jun-Tao
authors.initials: JT
authors.lastname: Cao
authors.affiliation: College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.
authors.forename: Gui-Fang
authors.initials: GF
authors.lastname: Shi
authors.affiliation: College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China. Electronic address: liuym9518@sina.com.
authors.forename: Yan-Ming
authors.initials: YM
authors.lastname: Liu
authors.affiliation: Xinyang Central Hospital, Xinyang 464000, China.
authors.forename: Yong-Hong
authors.initials: YH
authors.lastname: Chen
authors.affiliation: Xinyang Central Hospital, Xinyang 464000, China.
authors.forename: Shu-Wei
authors.initials: SW
authors.lastname: Ren
mesh_terms: D000077214:Becaplermin; D019187:Cadmium Compounds / Q000737:chemistry; D050091:Dendrimers / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods; D006801:Humans; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000379:methods*; D053761:Nanocomposites / Q000737:chemistry; D011073:Polyamines / Q000737:chemistry; D020574:Proto-Oncogene Proteins c-sis / Q000097:blood* / Q000737:chemistry; D045663:Quantum Dots; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013440:Sulfides / Q000737:chemistry
chemical_list: D019187:Cadmium Compounds; D050091:Dendrimers; C104700:PAMAM Starburst; C531249:Poly(amidoamine); D011073:Polyamines; D020574:Proto-Oncogene Proteins c-sis; D013440:Sulfides; C034939:cadmium sulfide; D000077214:Becaplermin
keywords: Aptasensor; Cds QDs–PAMAM–Apt composites; Electrochemiluminescence; MWCNTs–chitosan; PDGF-BB