title: (1)H, (13)C and (15)N resonance assignments of σ(S) activating protein Crl from Salmonella enterica serovar Typhimurium.
abstract: The general stress response in Enterobacteria, like Escherichia coli or Salmonella, is controlled by the transcription factor σ(S), encoded by the rpoS gene, which accumulates during stationary phase growth and associates with the core RNA polymerase enzyme (E) to promote transcription of genes involved in cell survival. Tight regulation of σ(S) is essential to preserve the balance between self-preservation under stress conditions and nutritional competence in the absence of stress. Whereas σ factors are generally inactivated upon interaction with anti-sigma proteins, σ(S) binding by the Crl protein facilitates the formation of the holoenzyme Eσ(S), and therefore σ(S)-controlled transcription. Previously, critical residues in both Crl and σ(S) were identified and assigned to the binding interface in the Crl-σ(S) complex. However, high-resolution structural data are missing to fully understand the molecular mechanisms underlying σ(S) activation by Crl, in particular the possible role of Crl in triggering domain rearrangements in the multi-domain protein σ(S). Here we provide the (1)H, (13)C and (15)N resonance assignments of Salmonella enterica serovar Typhimurium Crl, as a starting point for CrlSTM structure determination and further structural investigation of the CrlSTM-σ STM (S) complex.
authors.affiliation: Département de Microbiologie, Laboratoire Systèmes Macromoléculaires et Signalisation, Institut Pasteur, 25 rue du Docteur Roux, 75015, Paris, France.|CNRS ERL3526, rue du Docteur Roux, 75015, Paris, France.
authors.forename: Paola
authors.initials: P
authors.lastname: Cavaliere
authors.affiliation: Département de Microbiologie, Laboratoire Systèmes Macromoléculaires et Signalisation, Institut Pasteur, 25 rue du Docteur Roux, 75015, Paris, France.|CNRS ERL3526, rue du Docteur Roux, 75015, Paris, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Norel
authors.affiliation: Institut de Chimie des Substances Naturelles, CNRS UPR2301, 91190, Gif-sur-Yvette, France. christina.sizun@cnrs.fr.
authors.forename: Christina
authors.identifier: http://orcid.org/0000-0002-5760-2614
authors.initials: C
authors.lastname: Sizun
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry*; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009587:Nitrogen Isotopes; D011513:Proteus mirabilis / Q000378:metabolism; D066244:Proton Magnetic Resonance Spectroscopy; D012486:Salmonella typhimurium / Q000378:metabolism*; D016415:Sequence Alignment; D012808:Sigma Factor / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D009587:Nitrogen Isotopes; D012808:Sigma Factor
keywords: Crl; RNA polymerase; RpoS; Salmonella Typhimurium; Sigma factor; Stress response