title: Changed profile of microRNAs in acute lung injury induced by cardio-pulmonary bypass and its mechanism involved with SIRT1.
abstract: OBJECTIVE
  Acute lung injury (ALI) is a severe complication for patients undergoing cardiac surgery necessitating cardio-pulmonary bypass (CPB), however, the possible relationship between microRNAs change and ALI induced by CPB is still not completely understood.
  
  
  OBJECTIVE
  the aim of this study is to determine the microRNAs level changes in patients with ALI induced by CPB and its involved mechanism.
  
  
  METHODS
  We collected blood samples from 45 patients and performed microRNA microarray experiments to determine the microRNAs level changes in patients with ALI induced by CPB then the result was verified by quantitative real-time PCR (qRT-PCR). Plasma TNF-α level and respiration parameters including respiration index (RI) and oxygenation index (OI) were measured at five different time points before or after CPB. Meanwhile the correlationship between significantly changed microRNAs and TNF-α level and respiration parameters was analyzed. Further more, we transfected miR-320 mimic and inhibitor into A549 cells and observed the proliferation inhibition and apoptosis change caused by oxygen-glucose deprivation/reperfusion. Finally we using dual-luciferase reporter assay, qRT-PCR and western blot investigated the potential target of miR-320.
  
  
  RESULTS
  The level of miR-320 was higher in CPB caused ALI with the most significance. Correlation analysis found that the level of miR-320 was positively associated with TNF-α and RI (r = 0.649 and 0.564, P < 0.05), but negative correlated with OI (r = -0.638, P < 0.05). In A549 cells, up-regulated miR-320 induced proliferation inhibition and more apoptosis. SIRT1 may be a target of miR-320 and higher miR-320 resulted in lower expression of SIRT both in mRNA and protein level.
  
  
  CONCLUSIONS
  miR-320 may mediate the ALI after CPB in which alveolar epithelial cells are injured via down-regulating SIRT1.
authors.affiliation: Department of Cardiopulmonary Bypass, Second Hospital of Lanzhou University 82 Cui Ying Men Street, Lanzhou City 730030, Gansu, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Yang
authors.affiliation: Department of Cardiac Surgery, Second Hospital of Lanzhou University 82 Cui Ying Men Street, Lanzhou City 730030, Gansu, China.
authors.forename: Bingren
authors.initials: B
authors.lastname: Gao
authors.affiliation: Department of Cardiopulmonary Bypass, Second Hospital of Lanzhou University 82 Cui Ying Men Street, Lanzhou City 730030, Gansu, China.
authors.forename: Wansheng
authors.initials: W
authors.lastname: Wei
authors.affiliation: Department of Cardiopulmonary Bypass, Second Hospital of Lanzhou University 82 Cui Ying Men Street, Lanzhou City 730030, Gansu, China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Cardiopulmonary Bypass, Second Hospital of Lanzhou University 82 Cui Ying Men Street, Lanzhou City 730030, Gansu, China.
authors.forename: Li
authors.initials: L
authors.lastname: Pan
authors.affiliation: Department of Cardiopulmonary Bypass, Second Hospital of Lanzhou University 82 Cui Ying Men Street, Lanzhou City 730030, Gansu, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Cardiac Surgery, Second Hospital of Lanzhou University 82 Cui Ying Men Street, Lanzhou City 730030, Gansu, China.
authors.forename: Qiming
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Department of Cardiac Surgery, Second Hospital of Lanzhou University 82 Cui Ying Men Street, Lanzhou City 730030, Gansu, China.
authors.forename: Wensheng
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Cardiac Surgery, Second Hospital of Lanzhou University 82 Cui Ying Men Street, Lanzhou City 730030, Gansu, China.
authors.forename: Zhiyi
authors.initials: Z
authors.lastname: Xu
mesh_terms: D055371:Acute Lung Injury / Q000209:etiology / Q000378:metabolism*; D000293:Adolescent; D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000502:physiology; D002315:Cardiopulmonary Bypass / Q000009:adverse effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000502:physiology; D002648:Child; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D056564:Sirtuin 1 / Q000235:genetics / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D055815:Young Adult
chemical_list: D035683:MicroRNAs; D014409:Tumor Necrosis Factor-alpha; C447939:SIRT1 protein, human; D056564:Sirtuin 1
keywords: Acute lung injury; SIRT1; cardio-pulmonary bypass; microRNA