title: A fluorescent bisboronic acid compound that selectively labels cells expressing oligosaccharide Lewis X.
abstract: Two fluorescent diboronic acid compounds (6a and 6b) with a dipeptide linker were synthesized as potential sensors for cell surface saccharide Lewis X (Le(X)). Compound 6a with a dipeptide (H-Asp-Ala-) as the linker was found to selectively label CHOFUT4 cells, which express Le(x), at micromolar concentrations, while non-Le(x)-expressing control cells were not labeled.
authors.affiliation: Department of Chemistry and Center for Diagnostics and Therapeutics, Georgia State University, Atlanta, GA 30303, USA.
authors.forename: Xingming
authors.initials: X
authors.lastname: Gao
authors.affiliation: Department of Chemistry and Center for Diagnostics and Therapeutics, Georgia State University, Atlanta, GA 30303, USA.
authors.forename: Mengyuan
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Department of Pediatrics, University of North Carolina, Chapel Hill, NC 27599-7220, USA.
authors.forename: Haiying
authors.initials: H
authors.lastname: Fan
authors.affiliation: Department of Chemistry and Center for Diagnostics and Therapeutics, Georgia State University, Atlanta, GA 30303, USA.
authors.forename: Wenqian
authors.initials: W
authors.lastname: Yang
authors.affiliation: Department of Chemistry and Center for Diagnostics and Therapeutics, Georgia State University, Atlanta, GA 30303, USA.
authors.forename: Weijuan
authors.initials: W
authors.lastname: Ni
authors.affiliation: Department of Chemistry and Center for Diagnostics and Therapeutics, Georgia State University, Atlanta, GA 30303, USA.
authors.forename: Vishnu V R
authors.initials: VV
authors.lastname: Karnati
authors.affiliation: Department of Chemistry and Center for Diagnostics and Therapeutics, Georgia State University, Atlanta, GA 30303, USA.
authors.forename: Shouhai
authors.initials: S
authors.lastname: Gao
authors.affiliation: Department of Pediatrics, University of North Carolina, Chapel Hill, NC 27599-7220, USA.
authors.forename: John
authors.initials: J
authors.lastname: Carson
authors.affiliation: Department of Pediatrics, University of North Carolina, Chapel Hill, NC 27599-7220, USA. Electronic address: bwwmd@med.unc.edu.
authors.forename: Brent
authors.initials: B
authors.lastname: Weston
authors.affiliation: Department of Chemistry and Center for Diagnostics and Therapeutics, Georgia State University, Atlanta, GA 30303, USA. Electronic address: wang@gsu.edu.
authors.forename: Binghe
authors.initials: B
authors.lastname: Wang
mesh_terms: D000818:Animals; D000873:Anthracenes / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D001897:Boronic Acids / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D004151:Dipeptides / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D005647:Fucosyltransferases / Q000737:chemistry / Q000378:metabolism; D007983:Lewis Blood Group Antigens; D008856:Microscopy, Fluorescence; D009844:Oligosaccharides / Q000235:genetics / Q000378:metabolism*
chemical_list: D000873:Anthracenes; D001897:Boronic Acids; D004151:Dipeptides; D005456:Fluorescent Dyes; D007983:Lewis Blood Group Antigens; C494219:Lewis a oligosaccharide; D009844:Oligosaccharides; D005647:Fucosyltransferases
keywords: Biomarker; Boronolectin; Optical imaging