title: In vitro differentiation of sperm from male germline stem cell.
abstract: In this study, we developed a model of sperm in vitro differentiation to study the mechanism of spermatogenesis. We isolated newborn male germ cells for in vitro differentiation. We found that after 4-5 weeks of culture, sperm-like cells were occasionally observed in cell co-culture and the feed-layer. After 1-2 weeks of culture followed by 4-5 weeks of co-culture, sperm-like cells were observed.
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, China.
authors.forename: P
authors.initials: P
authors.lastname: Zheng
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, China.
authors.forename: X W
authors.initials: XW
authors.lastname: Zhao
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, China.
authors.forename: X M
authors.initials: XM
authors.lastname: Zheng
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, China.
authors.forename: A
authors.initials: A
authors.lastname: Khalid
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, China gxzhang@neau.edu.cn.
authors.forename: G X
authors.initials: GX
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002417:Cattle; D002454:Cell Differentiation / Q000502:physiology; D018920:Coculture Techniques / Q000379:methods; D005854:Germ Cells; D008297:Male; D012708:Sertoli Cells / Q000166:cytology; D013091:Spermatogenesis / Q000502:physiology*; D013093:Spermatogonia / Q000166:cytology; D013094:Spermatozoa / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology; D046509:Tissue Culture Techniques / Q000379:methods