title: Quantum dots-based label-free fluorescence sensor for sensitive and non-enzymatic detection of caffeic acid.
abstract: We have developed a label-free fluorescence sensor for caffeic acid (CA) by the use of CdTe:Zn(2+) quantum dots (CdTe:Zn(2+) QDs) as an output signal. The principle of sensor is based on the fluorescence quenching and binding properties of Fe(2+) toward QDs and CA, respectively. To provide a fluorescence turn-on mode for CA detection, Fe(2+) is first mixed with QDs solution, leading to a low fluorescence emission. With the addition of CA, the fluorescence of QDs is recovered due to the strong binding interaction between CA and Fe(2+). Thus, a QDs-based label-free fluorescence sensor, designed in a simple mix-and-detect format, is established for CA detection. This study demonstrated here not only offers simple, sensitive and non-enzymatic detection method for CA, but also brings to light a new application of QDs in the food analysis.
authors.affiliation: Department of Product Processing and Nutriology, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Ministry of Agriculture Key Laboratory of Oil Crops Biology, Wuhan 430062, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Xiang
authors.affiliation: Institute of Agro-Products Processing and Nuclear-Agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan 430064, Hubei Province, China.
authors.forename: Jianbin
authors.initials: J
authors.lastname: Shi
authors.affiliation: Department of Product Processing and Nutriology, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Ministry of Agriculture Key Laboratory of Oil Crops Biology, Wuhan 430062, China. Electronic address: xiangshi19850130@163.com.
authors.forename: Fenghong
authors.initials: F
authors.lastname: Huang
authors.affiliation: Department of Product Processing and Nutriology, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Ministry of Agriculture Key Laboratory of Oil Crops Biology, Wuhan 430062, China.
authors.forename: Mingming
authors.initials: M
authors.lastname: Zheng
authors.affiliation: Department of Product Processing and Nutriology, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Ministry of Agriculture Key Laboratory of Oil Crops Biology, Wuhan 430062, China.
authors.forename: Qianchun
authors.initials: Q
authors.lastname: Deng
mesh_terms: D029741:Brassica rapa / Q000737:chemistry; D002104:Cadmium / Q000737:chemistry; D002109:Caffeic Acids / Q000032:analysis* / Q000737:chemistry; D005504:Food Analysis / Q000379:methods; D007501:Iron / Q000737:chemistry / Q000378:metabolism; D045663:Quantum Dots / Q000737:chemistry; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D013056:Spectrophotometry, Ultraviolet; D013691:Tellurium / Q000737:chemistry; D015032:Zinc / Q000737:chemistry
chemical_list: D002109:Caffeic Acids; D002104:Cadmium; D007501:Iron; D015032:Zinc; D013691:Tellurium; C040048:caffeic acid
keywords: Caffeic acid; Fe(2+); Fluorescence sensor; Quantum dots; Turn-on