title: Isolation and characterisation of cDNA encoding a wheat heavy metal-associated isoprenylated protein involved in stress responses.
abstract: In cells, metallochaperones are important proteins that safely transport metal ions. Heavy metal-associated isoprenylated plant proteins (HIPPs) are metallochaperones that contain a metal binding domain and a CaaX isoprenylation motif at the carboxy-terminal end. To investigate the roles of wheat heavy metal-associated isoprenylated plant protein (TaHIPP) genes in plant development and in stress responses, we isolated cDNA encoding the wheat TaHIPP1 gene, which contains a heavy metal-associated domain, nuclear localisation signals and an isoprenylation motif (CaaX motif). Quantitative real-time PCR analysis indicated that the TaHIPP1 gene was differentially expressed under biotic and abiotic stresses. Specifically, TaHIPP1 expression was up-regulated by ABA exposure or wounding. Additionally, TaHIPP1 over-expression in yeast (Schizosaccharomyces pombe) significantly increased the cell growth rate under Cu(2+) and high salinity stresses. The nuclear localisation of the protein was confirmed with confocal laser scanning microscopy of epidermal onion cells after particle bombardment with chimeric TaHIPP1-GFP constructs. In addition, TaHIPP1 was shown to enhance the susceptibility of wheat to Pst as determined by virus-induced gene silencing. These data indicate that TaHIPP1 is an important component in defence signalling pathways and may play a crucial role in the defence response of wheat to biotic and certain abiotic stresses.
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Science, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: H
authors.initials: H
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Science, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: C
authors.initials: C
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Science, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: H
authors.initials: H
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: X
authors.initials: X
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Science, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Science, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: X
authors.initials: X
authors.lastname: Duan
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: G
authors.initials: G
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: L
authors.initials: L
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Kang
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D000222:Adaptation, Physiological; D000595:Amino Acid Sequence; D003300:Copper / Q000378:metabolism; D018076:DNA, Complementary / Q000032:analysis; D015870:Gene Expression; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D054884:Host-Pathogen Interactions; D056511:Metallochaperones / Q000235:genetics* / Q000378:metabolism; D019216:Metals, Heavy / Q000378:metabolism; D008969:Molecular Sequence Data; D010935:Plant Diseases; D018515:Plant Leaves; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D054876:Prenylation; D054712:Salinity; D012568:Schizosaccharomyces / Q000378:metabolism; D012965:Sodium Chloride / Q000378:metabolism; D013312:Stress, Physiological; D015533:Transcriptional Activation; D014908:Triticum / Q000235:genetics* / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D056511:Metallochaperones; D019216:Metals, Heavy; D010940:Plant Proteins; D012965:Sodium Chloride; D000040:Abscisic Acid; D003300:Copper
keywords: Puccinia striiformis f. sp. Tritici; heavy metal-associated isoprenylated plant protein; stress response; virus-induced gene silencing; wheat