title: The Bacterial Component Flagellin Induces Anti-Sepsis Protection Through TLR-5, IL-1RN and VCAN During Polymicrobial Sepsis in Mice.
abstract: BACKGROUND
  The present study was designed to observe the effects of the bacterial component flagellin on anti-sepsis protection through TLR-5, VCAN and IL-1RN.
  
  
  METHODS
  A clinically relevant model of sepsis was induced by cecal ligation and puncture (CLP). An in vitro culture of endothelial cells was analyzed.
  
  
  RESULTS
  Flagellin induced anti-sepsis protection through inhibition of inflammation and induction of endothelial proliferation by down-regulating the expression of TLR 3, TLR 4, and IL-1RN and promoting the expression of VCAN in mice 24 h post-CLP. In vitro, flagellin promoted the proliferation of endothelial cells. These effects could be inhibited by transfection of endothelial cells with VCAN siRNA or IL-1RN over-expression constructs. VCAN expression decreased after transfection of the cells with an IL-1RN over-expression construct and increased after transfection of the cells with an IL-1RN siRNA construct. IL-1RN expression remained unchanged after transfection of the cells with VCAN over-expression or siRNA constructs.
  
  
  CONCLUSIONS
  These data suggest that flagellin pretreatment promoted anti-sepsis protection through the TLR-5, IL-1RN and VCAN pathway. This pathway is necessary to mediate endothelial repair and thereby promote survival following sepsis challenge.
authors.affiliation: Institute of Pathology, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Zhu
authors.forename: Guangjie
authors.initials: G
authors.lastname: Duan
authors.forename: Lang
authors.initials: L
authors.lastname: Lang
authors.forename: Yao
authors.initials: Y
authors.lastname: Liu
authors.forename: Junmin
authors.initials: J
authors.lastname: Zhu
authors.forename: Hu
authors.initials: H
authors.lastname: Wang
authors.forename: Yousheng
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D005408:Flagellin / Q000627:therapeutic use*; D005786:Gene Expression Regulation / Q000187:drug effects; D053590:Interleukin 1 Receptor Antagonist Protein / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D008168:Lung / Q000187:drug effects / Q000382:microbiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018805:Sepsis / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D051198:Toll-Like Receptor 5 / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D053675:Versicans / Q000032:analysis / Q000235:genetics / Q000276:immunology*
chemical_list: D000893:Anti-Inflammatory Agents; C507764:Il1rn protein, mouse; D053590:Interleukin 1 Receptor Antagonist Protein; D051198:Toll-Like Receptor 5; C508323:Vcan protein, mouse; D053675:Versicans; D005408:Flagellin