title: Oxidative modification induced by photodynamic therapy with Photofrin®II and 2-methoxyestradiol in human ovarian clear carcinoma (OvBH-1) and human breast adenocarcinoma (MCF-7) cells.
abstract: Ovarian cancer is among the most lethal cancers in women. The successful anticancer treatment depends on the effectiveness of cytotoxic effect of applied therapeutic procedures either alone or in combination with other treatments. Photodynamic therapy (PDT) is a relatively new method of anticancer therapy. Its dominant mechanism of action is the over-production of reactive oxygen species induced by oxidative stress in malignant cells, which attack lipid membranes, proteins and nucleic acids. One of the important mechanisms is induction of unfolded protein response, ubiquitin-proteasome pathway of protein degradation. The aim of this study was to evaluate the cytotoxic effect of various protective enzymes in ovarian carcinoma clear cell line in comparison to the model breast cell line after photodynamic reaction and photodynamic reaction with 2-methoxyestradiol (2-Me). Human malignant ovarian cell line (OvBH-1) was used and human breast adenocarcinoma cells (MCF-7) were used as a control. Photodynamic reaction (PDR) with Photofrin(®)II and Ph(®)II with 2-Me was performed. The expression of protective proteins by immunocytochemistry (HSP70 and iNOS) and western blot (Hsp27 and Hsp70) methods was evaluated directly, 3 and 6 h after PDR. The changes in cells' cytoskeleton were evaluated using immunofluorescence by confocal microscopy. The expression of iNOS was observed for both experiments with differential intensity and quantity. A higher expression of Hsp70 in MCF-7 cells was observed than in OvBh-1 cells. The reorganization of cytoskeleton and nucleus was observed after 3 and 6 h after exposition to light.
authors.affiliation: Department of Medical Biochemistry, Wroclaw Medical University, Chałubińskiego 10, 50-368 Wrocław, Poland.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Saczko
authors.affiliation: Department of Medical Biochemistry, Wroclaw Medical University, Chałubińskiego 10, 50-368 Wrocław, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Choromańska
authors.affiliation: Department of Medical Biochemistry, Wroclaw Medical University, Chałubińskiego 10, 50-368 Wrocław, Poland.
authors.forename: Nina
authors.initials: N
authors.lastname: Rembiałkowska
authors.affiliation: Department of Animal Developmental Biology, University of Wrocław, 21 Sienkiewicza Street, 50-335 Wrocław, Poland.
authors.forename: Magda
authors.initials: M
authors.lastname: Dubińska-Magiera
authors.affiliation: Department of Medical Biochemistry, Wroclaw Medical University, Chałubińskiego 10, 50-368 Wrocław, Poland.
authors.forename: Iwona
authors.initials: I
authors.lastname: Bednarz-Misa
authors.affiliation: Department of Pathomorphology and Clinical Cytology, Medical University, Borowska 213 St., 50-556 Wroclaw, Poland.
authors.forename: Julia
authors.initials: J
authors.lastname: Bar
authors.affiliation: Department of Medical Biochemistry, Wroclaw Medical University, Chałubińskiego 10, 50-368 Wrocław, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Marcinkowska
authors.affiliation: Department of Medical Biochemistry, Wroclaw Medical University, Chałubińskiego 10, 50-368 Wrocław, Poland. Electronic address: julita.kulbacka@umed.wroc.pl.
authors.forename: Julita
authors.initials: J
authors.lastname: Kulbacka
mesh_terms: D000077584:2-Methoxyestradiol; D000230:Adenocarcinoma / Q000188:drug therapy* / Q000473:pathology; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D017323:Dihematoporphyrin Ether / Q000494:pharmacology / Q000627:therapeutic use*; D004958:Estradiol / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D005260:Female; D055551:HSP27 Heat-Shock Proteins / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010051:Ovarian Neoplasms / Q000188:drug therapy* / Q000473:pathology; D010084:Oxidation-Reduction / Q000187:drug effects; D010778:Photochemotherapy
chemical_list: D055551:HSP27 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D004958:Estradiol; D000077584:2-Methoxyestradiol; D017323:Dihematoporphyrin Ether; D052247:Nitric Oxide Synthase Type II
keywords: 2-Methoxyestradiol; Breast adenocarcinoma; Cytoskeleton; Heat shock proteins; Ovarian carcinoma; Photodynamic reaction; Photofrin(®)II