title: Effects of L-carnitine on follicular survival and graft function following autotransplantation of cryopreserved-thawed ovarian tissues.
abstract: The aim of this study was to investigate the effects of L-carnitine (LC) on follicular survival and ovarian function following cryopreservation-thawing and autotransplantation of ovarian tissues. ICR mice were divided into three groups: control; saline group (cryopreservation+autograft+saline); and LC group (cryopreservation+autograft+L-carnitine). The ovarian tissues from control group, saline group, and LC group were histological assessed. There were no significant differences in the percentage of morphologically normal primordial follicles between the LC group and the saline group. After 28 days of autotransplantation, apoptosis rates, plasma malondialdehyde (MDA), progesterone (P4) and estradiol (E2) concentrations, and follicular densities of grafts were evaluated. Apoptosis rate and the concentration of MDA in the LC group were significantly lower than those in the saline group. The concentration of E2 and follicular densities of grafts in LC group were significantly higher than that in saline group. LC inhibits follicle apoptosis and increases follicular survival and function of ovarian graft.
authors.affiliation: Radiology Department, Jinan Central Hospital Affiliated to Shandong University, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Nephrology, Jinan Central Hospital Affiliated to Shandong University, China.
authors.forename: Shao-Mei
authors.initials: SM
authors.lastname: Wang
authors.affiliation: Outpatient Surgery, Jinan Central Hospital Affiliated to Shandong University, China.
authors.forename: Ping-Bao
authors.initials: PB
authors.lastname: Yao
authors.affiliation: Department of Reproductive Medicine, Hospital for Maternity and Child Care of Jinan City, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Reproductive Medicine, Hospital for Maternity and Child Care of Jinan City, China.
authors.forename: Ya-Jie
authors.initials: YJ
authors.lastname: Zhang
authors.affiliation: Department of Reproductive Medicine, Hospital for Maternity and Child Care of Jinan City, China.
authors.forename: Ru-Xin
authors.initials: RX
authors.lastname: Chen
authors.affiliation: Department of Reproductive Medicine, Hospital for Maternity and Child Care of Jinan City, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Department of Reproductive Medicine, Hospital for Maternity and Child Care of Jinan City, China. Electronic address: jmzjnfy@163.com.
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002331:Carnitine / Q000494:pharmacology*; D015925:Cryopreservation / Q000379:methods; D004958:Estradiol / Q000097:blood; D005260:Female; D006085:Graft Survival / Q000187:drug effects*; D008315:Malondialdehyde / Q000097:blood; D051379:Mice; D008813:Mice, Inbred ICR; D006080:Ovarian Follicle / Q000187:drug effects* / Q000637:transplantation*; D011374:Progesterone / Q000097:blood; D011897:Random Allocation; D014021:Tissue Preservation / Q000379:methods; D014182:Transplantation, Autologous; D058989:Vitrification
chemical_list: D011374:Progesterone; D004958:Estradiol; D008315:Malondialdehyde; D002331:Carnitine
keywords: Cryopreservation; Ischemia–reperfusion; Ovarian tissue; Transplantation; l-Carnitine