title: [Effect of astaxanthin on oxidative stress of red blood cells and peroxidation damage of membrane].
abstract: OBJECTIVE
  To explore the effect of astaxanthin (ASTA) on oxidative stress of intra- and extra- red blood cells during stored period and the protective function for cell membrane.
  
  
  METHODS
  The blood of volunteers was collected to prepare suspended red blood cells without leukocytes. Then the red blood cells were randomly divided into group A, group B, group C and group D. The ASTA was added into MAP preservation solution of group B, group C and group D, the final concentration of ASTA was 5, 10 and 20 µmol/L respectively. Group A was used as control group, in which only the dissolved liquid DMSO of ASTA was added. The red blood cells were stored in refrigerator at 2 °C-6 °C. On day 7, 14, 28 and day 42 of storage, the content of reactive oxygen species (ROS) in red blood cells was detected by fluorescence microplate reader. The content of malondialdehyde (MDA) was detected with TBA method. The content of hydrogen peroxide (H2O2) outside cell was detected with spectrophotometric method. The mean corpuscular volume(MCV) was detected with blood cell analyzer. The content of free hemoglobin(FHb) was detected with chemical colorimetry.
  
  
  RESULTS
  The ROS, MDA, FHb and H2O2 levels in B, C and D groups were lower than those in control group during the stored period. On day 7 and 14 of storage, among group B, group C, group D and group A, the MCV showed no difference in comparison with control group. On day 28 and 42 of storage, the MCV in B, C and D groups was lower than that in control group.
  
  
  CONCLUSIONS
  The ASTA can reduce the oxidative stress level of stored red blood cells inside and outside, relieve the peroxidation damage of cell membrane.
authors.affiliation: Department of Laboratorial Examination, Hebei Blood Center, Shijiazhuang 050071, Hebei Province, China.
authors.forename: Su-Ling
authors.initials: SL
authors.lastname: Wang
authors.affiliation: Department of Laboratorial Examination, Hebei Blood Center, Shijiazhuang 050071, Hebei Province, China.
authors.forename: Lu-Jun
authors.initials: LJ
authors.lastname: He
authors.affiliation: Department of Laboratorial Examination, The Forth Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China.
authors.forename: Tian-Bo
authors.initials: TB
authors.lastname: He
authors.affiliation: Department of Laboratorial Examination, Hebei Blood Center, Shijiazhuang 050071, Hebei Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Han
authors.affiliation: Department of Anatomy, Hebei Medical University, Shijiazhuang 050017, Hebei Province, China. E-mail: wangqie1012@163. com.
authors.forename: Qie
authors.initials: Q
authors.lastname: Wang
mesh_terms: D004906:Erythrocyte Count; D004912:Erythrocytes; D006801:Humans; D006861:Hydrogen Peroxide; D007962:Leukocytes; D018384:Oxidative Stress; D017382:Reactive Oxygen Species; D024341:Xanthophylls
chemical_list: D017382:Reactive Oxygen Species; D024341:Xanthophylls; C005948:astaxanthine; D006861:Hydrogen Peroxide