title: [Study on delivery efficiency and cytotoxicity of Hela cells with mPEG-PLGA-BSA-FITC-NPs nanocarrier].
abstract: OBJECTIVE
  To construct and obtain ideal protein delivery vectors by researching the delivery efficiency and cytotoxicity to Hela cells using mPEG-PLGA-BSA-FITC-NPs.
  
  
  METHODS
  The mPEG-PLGA nanoparticle was obtained through surface modification of PLGA with PEG, and deliver BSA-FITC into Hela cells in vitro. The positive cells were counted by Laser scanning confocal microscopy and the survival rate of Hela cells was calculated by MTT assay at different time points.
  
  
  RESULTS
  mPEG-PLGA-BSA-FITC-NPs shows the classic nanometer size, and the encapsulation efficiency reached 51. 2%. At the same time, the nanoparticles possess characteristics of slow release. By optimizing the delivery conditions, the highest efficiency of mPEG-PLGA-BSA-FITC-NPs was above 65.2%, and the cellular viability was about 85.7%.
  
  
  CONCLUSIONS
  mPEG-PLGA-BSA-FITC-NPs nanoparticles can successfully carry the target protein into cells as safe and effective as novel delivery materials of protein in vitro, and has shown slow release characteristics. The mPEG-PLGA-BSA-FITC-NPs provide ideal delivery vector for future application in clinical treatment of disease using nano-materials.
authors.forename: Zhiting
authors.initials: Z
authors.lastname: Chen
authors.forename: Nan
authors.initials: N
authors.lastname: Wu
authors.forename: Xiongwei
authors.initials: X
authors.lastname: Deng
authors.forename: Fangyuan
authors.initials: F
authors.lastname: Wang
authors.forename: Kun
authors.initials: K
authors.lastname: Li
authors.forename: Weiwei
authors.initials: W
authors.lastname: Guo
authors.forename: Riyuan
authors.initials: R
authors.lastname: Liu
authors.forename: Shuolong
authors.initials: S
authors.lastname: Yuan
authors.forename: Jiakun
authors.initials: J
authors.lastname: Zhang
authors.forename: Shiming
authors.initials: S
authors.lastname: Yang
mesh_terms: D004337:Drug Carriers; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives; D006367:HeLa Cells; D006801:Humans; D053758:Nanoparticles; D010316:Particle Size; D011091:Polyesters; D011092:Polyethylene Glycols; D012710:Serum Albumin, Bovine
chemical_list: D004337:Drug Carriers; D011091:Polyesters; C039217:fluorescein isothiocyanate bovine serum albumin; C558447:methoxypolyethyleneglycol-poly(lactic-co-glycolic acid); D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D016650:Fluorescein-5-isothiocyanate