title: Advanced biopolymer-coated drug-releasing titania nanotubes (TNTs) implants with simultaneously enhanced osteoblast adhesion and antibacterial properties.
abstract: Here, we report on the development of advanced biopolymer-coated drug-releasing implants based on titanium (Ti) featuring titania nanotubes (TNTs) on its surface. These TNT arrays were fabricated on the Ti surface by electrochemical anodization, followed by the loading and release of a model antibiotic drug, gentamicin. The osteoblastic adhesion and antibacterial properties of these TNT-Ti samples are significantly improved by loading antibacterial payloads inside the nanotubes and modifying their surface with two biopolymer coatings (PLGA and chitosan). The improved osteoblast adhesion and antibacterial properties of these drug-releasing TNT-Ti samples are confirmed by the adhesion and proliferation studies of osteoblasts and model Gram-positive bacteria (Staphylococcus epidermidis). The adhesion of these cells on TNT-Ti samples is monitored by fluorescence and scanning electron microscopies. Results reveal the ability of these biopolymer-coated drug-releasing TNT-Ti substrates to promote osteoblast adhesion and proliferation, while effectively preventing bacterial colonization by impeding their proliferation and biofilm formation. The proposed approach could overcome inherent problems associated with bacterial infections on Ti-based implants, simultaneously enabling the development of orthopedic implants with enhanced and synergistic antibacterial functionalities and bone cell promotion.
authors.affiliation: School of Chemical Engineering, The University of Adelaide, Adelaide, SA 5005, Australia.
authors.forename: Tushar
authors.initials: T
authors.lastname: Kumeria
authors.affiliation: Sansom Institute for Health Research, School of Pharmacy and Medical Sciences, University of South Australia, Frome Road, Adelaide, SA 5000, Australia.
authors.forename: Htwe
authors.initials: H
authors.lastname: Mon
authors.affiliation: School of Chemical Engineering, The University of Adelaide, Adelaide, SA 5005, Australia.
authors.forename: Moom Sinn
authors.initials: MS
authors.lastname: Aw
authors.affiliation: School of Chemical Engineering, The University of Adelaide, Adelaide, SA 5005, Australia.
authors.forename: Karan
authors.initials: K
authors.lastname: Gulati
authors.affiliation: School of Chemical Engineering, The University of Adelaide, Adelaide, SA 5005, Australia.
authors.forename: Abel
authors.initials: A
authors.lastname: Santos
authors.affiliation: Mawson Institute, University of South Australia, Mawson Lakes, SA 5095, Australia. Electronic address: hans.griesser@unisa.edu.au.
authors.forename: Hans J
authors.initials: HJ
authors.lastname: Griesser
authors.affiliation: School of Chemical Engineering, The University of Adelaide, Adelaide, SA 5005, Australia. Electronic address: dusan.losic@adelaide.edu.au.
authors.forename: Dusan
authors.initials: D
authors.lastname: Losic
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development; D001704:Biopolymers / Q000737:chemistry*; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003692:Delayed-Action Preparations / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology; D004343:Drug Implants; D005839:Gentamicins / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D006801:Humans; D008823:Micelles; D050296:Microbial Viability / Q000187:drug effects; D008855:Microscopy, Electron, Scanning; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000502:physiology; D014025:Titanium / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D001704:Biopolymers; D020099:Coated Materials, Biocompatible; D003692:Delayed-Action Preparations; D004343:Drug Implants; D005839:Gentamicins; D008823:Micelles; D014025:Titanium
keywords: Biopolymer coating; Bone implants; Drug release; Osteoblast cells; Staphylococcus epidermidis; Titania nanotubes