title: Failure of intravenous or intracardiac delivery of mesenchymal stromal cells to improve outcomes after focal traumatic brain injury in the female rat.
abstract: Mesenchymal stromal cells secrete a variety of anti-inflammatory factors and may provide a regenerative medicine option for the treatment of traumatic brain injury. The present study investigates the efficacy of multiple intravenous or intracardiac administrations of rat mesenchymal stromal cells or human mesenchymal stromal cells in female rats after controlled cortical impact by in vivo MRI, neurobehavior, and histopathology evaluation. Neither intravenous nor intracardiac administration of mesenchymal stromal cells derived from either rats or humans improved MRI measures of lesion volume or neurobehavioral outcome compared to saline treatment. Few mesenchymal stromal cells (<0.0005% of injected dose) were found within 3 days of last dosage at the site of injury after either delivery route, with no mesenchymal stromal cells being detectable in brain at 30 or 56 days post-injury. These findings suggest that non-autologous mesenchymal stromal cells therapy via intravenous or intracardiac administration is not a promising treatment after focal contusion traumatic brain injury in this female rodent model.
authors.affiliation: Center for Neuroscience and Regenerative Medicine, Uniformed Services University of the Health Sciences, Bethesda, Maryland, United States of America; Frank Laboratory, National Institutes of Health, Bethesda, Maryland, United States of America.
authors.forename: L Christine
authors.initials: LC
authors.lastname: Turtzo
authors.affiliation: Frank Laboratory, National Institutes of Health, Bethesda, Maryland, United States of America.
authors.forename: Matthew D
authors.initials: MD
authors.lastname: Budde
authors.affiliation: Frank Laboratory, National Institutes of Health, Bethesda, Maryland, United States of America.
authors.forename: Dana D
authors.initials: DD
authors.lastname: Dean
authors.affiliation: Center for Neuroscience and Regenerative Medicine, Uniformed Services University of the Health Sciences, Bethesda, Maryland, United States of America; Frank Laboratory, National Institutes of Health, Bethesda, Maryland, United States of America.
authors.forename: Eric M
authors.initials: EM
authors.lastname: Gold
authors.affiliation: Frank Laboratory, National Institutes of Health, Bethesda, Maryland, United States of America.
authors.forename: Bobbi K
authors.initials: BK
authors.lastname: Lewis
authors.affiliation: Center for Neuroscience and Regenerative Medicine, Uniformed Services University of the Health Sciences, Bethesda, Maryland, United States of America; Frank Laboratory, National Institutes of Health, Bethesda, Maryland, United States of America.
authors.forename: Lindsay
authors.initials: L
authors.lastname: Janes
authors.affiliation: Center for Neuroscience and Regenerative Medicine, Uniformed Services University of the Health Sciences, Bethesda, Maryland, United States of America; Frank Laboratory, National Institutes of Health, Bethesda, Maryland, United States of America.
authors.forename: Jacob
authors.initials: J
authors.lastname: Lescher
authors.affiliation: Frank Laboratory, National Institutes of Health, Bethesda, Maryland, United States of America.
authors.forename: Tiziana
authors.initials: T
authors.lastname: Coppola
authors.affiliation: Department of Medical and Clinical Psychology, Uniformed Services University of the Health Sciences, Bethesda, Maryland, United States of America.
authors.forename: Angela
authors.initials: A
authors.lastname: Yarnell
authors.affiliation: Department of Medical and Clinical Psychology, Uniformed Services University of the Health Sciences, Bethesda, Maryland, United States of America.
authors.forename: Neil E
authors.initials: NE
authors.lastname: Grunberg
authors.affiliation: Frank Laboratory, National Institutes of Health, Bethesda, Maryland, United States of America; National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, United States of America.
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Frank
mesh_terms: D061605:Administration, Intravenous; D000818:Animals; D001921:Brain / Q000166:cytology / Q000473:pathology*; D001930:Brain Injuries / Q000628:therapy*; D062906:Cardiac Catheters; D064987:Cell- and Tissue-Based Therapy / Q000379:methods*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008279:Magnetic Resonance Imaging; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology; D051381:Rats; D017208:Rats, Wistar; D017211:Treatment Failure