title: Profilin 2 promotes migration, invasion, and stemness of HT29 human colorectal cancer stem cells.
abstract: We investigated the role of profilin 2 in the stemness, migration, and invasion of HT29 cancer stem cells (CSCs). Increased and decreased levels of profilin 2 significantly enhanced and suppressed the self-renewal, migration, and invasion ability of HT29 CSCs, respectively. Moreover, profilin 2 directly regulated the expression of stemness markers (CD133, SOX2, and β-catenin) and epithelial mesenchymal transition (EMT) markers (E-cadherin and snail). CD133 and β-catenin were up-regulated by overexpression of profilin 2 and down-regulated by depletion of profilin 2. SOX2 was decreased by profilin 2 depletion. E-cadherin was not influenced by profilin 2- overexpression but increased by profilin 2- knockdown. The expression of snail was suppressed by profilin 2- knockdown. We speculated that stemness and the EMT are closely linked through profilin 2-related pathways. Therefore, this study indicates that profilin 2 affects the metastatic potential and stemness of colorectal CSCs by regulating EMT- and stemness-related proteins.
authors.affiliation: a College of Life Sciences and Biotechnology , Korea University , Seoul 136-701 , Korea.
authors.forename: Min-Jung
authors.initials: MJ
authors.lastname: Kim
authors.forename: Yoo-Sun
authors.initials: YS
authors.lastname: Lee
authors.forename: Gi-Yeon
authors.initials: GY
authors.lastname: Han
authors.forename: Han-Na
authors.initials: HN
authors.lastname: Lee
authors.forename: Chiyoung
authors.initials: C
authors.lastname: Ahn
authors.forename: Chan-Wha
authors.initials: CW
authors.lastname: Kim
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D019073:HT29 Cells; D006801:Humans; D009361:Neoplasm Invasiveness / Q000235:genetics; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D014411:Neoplastic Stem Cells; D051304:Profilins / Q000096:biosynthesis / Q000235:genetics*; D060449:Wnt Signaling Pathway / Q000235:genetics; D051176:beta Catenin / Q000096:biosynthesis
chemical_list: D009363:Neoplasm Proteins; C495741:PFN2 protein, human; D051304:Profilins; D051176:beta Catenin
keywords: Cancer stem cells (CSCs); Invasion; Migration; Profilin 2; Stemness