title: Ion-exchange vs reversed-phase chromatography for separation and determination of basic psychotropic drugs.
abstract: Ion exchange chromatography, an alternative to reversed-phase (RP) chromatography, is described in this paper. We aimed to obtain optimal conditions for the separation of basic drugs because silica-based RP stationary phases show silanol effect and make the analysis of basic analytes hardly possible. The retention, separation selectivity, symmetry of peaks and system efficiency were examined in different eluent systems containing different types of buffers at acidic pH and with the addition of organic modifiers: methanol and acetonitrile. The obtained results reveal a large influence of the salt cation used for buffer preparation and the type of organic modifier on the retention behavior of the analytes. These results were also compared with those obtained on an XBridge C18 column. The obtained results demonstrated that SCX stationary phases can be successfully used as alternatives to C18 stationary phases in the separation of basic compounds. The most selective and efficient chromatographic systems were applied for the quantification of some psychotropic drugs in fortified human serum samples.
authors.affiliation: Department of Inorganic Chemistry, Medical University of Lublin, Chodzki 4a, Lublin, 20-093, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Petruczynik
authors.affiliation: Department of Inorganic Chemistry, Medical University of Lublin, Chodzki 4a, Lublin, 20-093, Poland.
authors.forename: Karol
authors.initials: K
authors.lastname: Wróblewski
authors.affiliation: Department of Inorganic Chemistry, Medical University of Lublin, Chodzki 4a, Lublin, 20-093, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Deja
authors.affiliation: Department of Inorganic Chemistry, Medical University of Lublin, Chodzki 4a, Lublin, 20-093, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Waksmundzka-Hajnos
mesh_terms: D002852:Chromatography, Ion Exchange / Q000379:methods*; D056148:Chromatography, Reverse-Phase; D006801:Humans; D057230:Limit of Detection; D011619:Psychotropic Drugs / Q000097:blood* / Q000302:isolation & purification; D015203:Reproducibility of Results; D052616:Solid Phase Extraction
chemical_list: D011619:Psychotropic Drugs
keywords: IEC-HPLC; RP-HPLC; optimization; psychotropic drugs; quantitative analysis