title: Transcript profiling of aquaporins during basidiocarp development in Laccaria bicolor ectomycorrhizal with Picea glauca.
abstract: Sporocarp formation is part of the reproductive stage in the life cycle of many mycorrhizal macrofungi. Sporocarp formation is accompanied by a transcriptomic switch and profound changes in regulation of the gene families that play crucial roles in the sporocarp initiation and maturation. Since sporocarp growth requires efficient water delivery, in the present study, we investigated changes in transcript abundance of six fungal aquaporin genes that could be cloned from the ectomycorrhizal fungus Laccaria bicolor strain UAMH8232, during the initiation and development of its basidiocarp. Aquaporins are intrinsic membrane proteins facilitating the transmembrane transport of water and other small neutral molecules. In controlled-environment experiments, we induced basidiocarp formation in L. bicolor, which formed ectomycorrhizal associations with white spruce (Picea glauca) seedlings. We profiled transcript abundance corresponding to six fungal aquaporin genes at six different developmental stages of basidiocarp growth and development. We also compared physiological parameters of non-inoculated to mycorrhizal seedlings with and without the presence of basidiocarps. Two L. bicolor aquaporins--JQ585592, a functional channel for CO2, NO and H2O2, and JQ585595, a functional water channel--showed the greatest degree of upregulation during development of the basidiocarp. Our findings point to the importance of aquaporin-mediated transmembrane water and CO2 transport during distinct stages of basidiocarp development.
authors.affiliation: Department of Renewable Resources, University of Alberta, Edmonton, AB, Canada, T6G 2E3.
authors.forename: Hao
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Renewable Resources, University of Alberta, Edmonton, AB, Canada, T6G 2E3.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Navarro-Ródenas
authors.affiliation: Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada, T6G 2E9.
authors.forename: Janice E K
authors.initials: JE
authors.lastname: Cooke
authors.affiliation: Department of Renewable Resources, University of Alberta, Edmonton, AB, Canada, T6G 2E3. jzwiazek@ualberta.ca.
authors.forename: Janusz J
authors.initials: JJ
authors.lastname: Zwiazek
mesh_terms: D020346:Aquaporins / Q000235:genetics* / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D048690:Fruiting Bodies, Fungal / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D006861:Hydrogen Peroxide / Q000378:metabolism; D055399:Laccaria / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D038821:Mycorrhizae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D009589:Nitrogen Oxides / Q000378:metabolism; D028222:Picea / Q000235:genetics / Q000254:growth & development / Q000382:microbiology*; D018517:Plant Roots / Q000382:microbiology; D036226:Seedlings / Q000254:growth & development / Q000382:microbiology; D015854:Up-Regulation; D014867:Water / Q000378:metabolism
chemical_list: D020346:Aquaporins; D005656:Fungal Proteins; D009589:Nitrogen Oxides; D014867:Water; D002245:Carbon Dioxide; D006861:Hydrogen Peroxide
keywords: CO2 transport; Fruiting body; Fungal aquaporins; Fungal cell expansion and differentiation; Photosynthate transport; Sporocarp; Water transport