title: Effect of TBT and PAHs on CYP1A, AhR and Vitellogenin Gene Expression in the Japanese Eel, Anguilla japonica.
abstract: Gene expressions of cytochrome P4501A (CYP1A), aryl hydrocarbon receptor (AhR) and vitellogenin (Vg) by endocrine disruptors, benzo[α]pyrene (B[a]P) and tributyltin (TBT) were examined in cultured eel hepatocytes which were isolated from eels treated previously with B[a]P (10 mg/kg) or estradiol-17β (20 mg/kg) in vivo, and the relationship between CYP1A, AhR and Vg genes were studied. When the cultured eel hepatocytes were treated with B[a]P (10(-6)-10(-5) M) the gene expressions of CYP1A and AhR were enhanced in a concentration-dependent manner. However, when treated with TBT (10(-9)-10(-5) M) the gene expressions of CYP1A and AhR were suppressed at high concentrations (10(-6)-10(-5) M), while having no effects at low concentrations (10(-9)-10(-7) M). Gene expression of Vg was also suppressed by TBT in a concentration-dependent manner in cultured eel hepatocytes which was previously treated in vivo with estradiol-17β.
authors.affiliation: Dept. of Aquatic Life Medical Science, Sun Moon University, Asan 336-708, Korea.
authors.forename: Min Seop
authors.initials: MS
authors.lastname: Choi
authors.affiliation: Dept. of Aquatic Life Medical Science, Sun Moon University, Asan 336-708, Korea.
authors.forename: Se Ryun
authors.initials: SR
authors.lastname: Kwon
authors.affiliation: Dept. of Food Science, Sun Moon University, Asan 336-708, Korea.
authors.forename: Seong Hee
authors.initials: SH
authors.lastname: Choi
authors.affiliation: Dept. of Aquatic Life Medical Science, Sun Moon University, Asan 336-708, Korea.
authors.forename: Hyuk Chu
authors.initials: HC
authors.lastname: Kwon
keywords: AhR; B[a]P; CYPIA; Eel hepatocytes; TBT; Vitellogenin