title: Mosaic genome of endobacteria in arbuscular mycorrhizal fungi: Transkingdom gene transfer in an ancient mycoplasma-fungus association.
abstract: For more than 450 million years, arbuscular mycorrhizal fungi (AMF) have formed intimate, mutualistic symbioses with the vast majority of land plants and are major drivers in almost all terrestrial ecosystems. The obligate plant-symbiotic AMF host additional symbionts, so-called Mollicutes-related endobacteria (MRE). To uncover putative functional roles of these widespread but yet enigmatic MRE, we sequenced the genome of DhMRE living in the AMF Dentiscutata heterogama. Multilocus phylogenetic analyses showed that MRE form a previously unidentified lineage sister to the hominis group of Mycoplasma species. DhMRE possesses a strongly reduced metabolic capacity with 55% of the proteins having unknown function, which reflects unique adaptations to an intracellular lifestyle. We found evidence for transkingdom gene transfer between MRE and their AMF host. At least 27 annotated DhMRE proteins show similarities to nuclear-encoded proteins of the AMF Rhizophagus irregularis, which itself lacks MRE. Nuclear-encoded homologs could moreover be identified for another AMF, Gigaspora margarita, and surprisingly, also the non-AMF Mortierella verticillata. Our data indicate a possible origin of the MRE-fungus association in ancestors of the Glomeromycota and Mucoromycotina. The DhMRE genome encodes an arsenal of putative regulatory proteins with eukaryotic-like domains, some of them encoded in putative genomic islands. MRE are highly interesting candidates to study the evolution and interactions between an ancient, obligate endosymbiotic prokaryote with its obligate plant-symbiotic fungal host. Our data moreover may be used for further targeted searches for ancient effector-like proteins that may be key components in the regulation of the arbuscular mycorrhiza symbiosis.
authors.affiliation: Department of Biology, Ludwig-Maximilians-University Munich, 82152 Martinsried, Germany; gloria.torres@bio.lmu.de.
authors.forename: Gloria
authors.initials: G
authors.lastname: Torres-Cortés
authors.affiliation: Institute for Sustainable Plant Protection, Torino Unit, National Research Council, 10125 Torino, Italy;
authors.forename: Stefano
authors.identifier: http://orcid.org/0000-0002-2033-2286
authors.initials: S
authors.lastname: Ghignone
authors.affiliation: Department of Life Sciences and Systems Biology, University of Torino, 10125 Torino, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Bonfante
authors.affiliation: Department of Biology, Ludwig-Maximilians-University Munich, 82152 Martinsried, Germany;
authors.forename: Arthur
authors.initials: A
authors.lastname: Schüßler
mesh_terms: D001419:Bacteria / Q000235:genetics*; D018014:Gene Transfer Techniques; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D009030:Mosaicism; D038821:Mycorrhizae
keywords: Mycoplasma; arbuscular mycorrhizal fungi; endobacteria; horizontal gene transfer; intracellular symbiont