title: CE-MS-based serum fingerprinting to track evolution of type 2 diabetes mellitus.
abstract: Development of type 2 diabetes mellitus (T2DM) is preceded by insulin resistance (IR), which may evolve to impaired fasting glucose (IFG) and/or impaired glucose tolerance (IGT). IFG and IGT are considered as prediabetic states (PD). Prediabetes indicates the high risk for the future development of diabetes, it is estimated that up to 70% of prediabetics eventually develop T2DM. The risk of T2DM development is increased in overweight (OW) and obese (OB) people; however normal weight (NW) individuals also suffer from T2DM. The present study was designed to evaluate whether changes in polar metabolites induced by T2DM evolution are different between NW, overweight and obese individuals. CE-MS serum fingerprinting was performed on 197 serum samples obtained from OW, OB, and NW humans whom were IR, prediabetics, diabetics or with normal glucose homeostasis. Metabolic changes evoked by the progression of T2DM differ between obese, overweight, and normal weight subjects. Based on obtained results several metabolites can be proposed as a promising target to track T2DM evolution; BCAA in OW and NW humans, lysine in OB, while acetylcarnitine and methionine independently on body mass index. Validation of obtained results on larger population is required.
authors.affiliation: Clinical Research Centre, Medical University of Bialystok, Bialystok, Poland.
authors.forename: Michal
authors.initials: M
authors.lastname: Ciborowski
authors.affiliation: Clinical Research Centre, Medical University of Bialystok, Bialystok, Poland.
authors.forename: Edyta
authors.initials: E
authors.lastname: Adamska
authors.affiliation: Department of Inorganic and Analytical Chemistry, Medical University of Bialystok, Bialystok, Poland.|CEMBIO (Center for Metabolomics and Bioanalysis), Universidad CEU San Pablo, Facultad de Farmacia, Madrid, Spain.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Rusak
authors.affiliation: CEMBIO (Center for Metabolomics and Bioanalysis), Universidad CEU San Pablo, Facultad de Farmacia, Madrid, Spain.
authors.forename: Joanna
authors.initials: J
authors.lastname: Godzien
authors.affiliation: Clinical Research Centre, Medical University of Bialystok, Bialystok, Poland.
authors.forename: Juliusz
authors.initials: J
authors.lastname: Wilk
authors.affiliation: Clinical Research Centre, Medical University of Bialystok, Bialystok, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Citko
authors.affiliation: Clinical Research Centre, Medical University of Bialystok, Bialystok, Poland.
authors.forename: Witold
authors.initials: W
authors.lastname: Bauer
authors.affiliation: Department of Endocrinology, Diabetology and Internal Medicine, Medical University of Bialystok, Bialystok, Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Gorska
authors.affiliation: Clinical Research Centre, Medical University of Bialystok, Bialystok, Poland.|Department of Endocrinology, Diabetology and Internal Medicine, Medical University of Bialystok, Bialystok, Poland.
authors.forename: Adam
authors.initials: A
authors.lastname: Kretowski
keywords: CE-MS; Obesity; Serum metabolic fingerprinting; Type 2 diabetes mellitus