title: [Effects of methyl bromide fumigation on community structure of denitrifying bacteria with nitrousoxide reductase gene (nosZ) in soil].
abstract: OBJECTIVE
  We studied the effect of methyl bromide fumigation on soil edaphic denitrification.
  
  
  METHODS
  We adopted nosZ-PCR-RFLP (restriction fragment length polymorphism) method, nosZ-MPN-PCR (Most-Probable-Number- PCR) counting method and soil nitrate elimination rate method, to explore the effect of methyl bromide fumigation on community structure, quantity and activity of denitrifying bacteria in soil.
  
  
  RESULTS
  After methyl bromide fumigating soil for 100 d, soil denitrification did not change obviously (P > 0. 05). Margalef index, Shannon-wiener index and Evenness index had no significant difference (P > 0.05) in nosZ denitrifying bacterial communities between fumigated soil and the control. There were Rhodopsendomonas, Pseudomonas fluorescens, Herbacspirillum, uncultured bacterium partial in both of them. However, Azospirillum, Rhizobium melibei, Nitrosospira multiformis were exclusively found in the control, and Uncultured Azospirillum sp, Mesorhizobium sp were in fumigated one. Moreover, the number of denitrifying bacteria in the control resolved by nosZ-MPN-PCR (Most-Probable-Number-PCR) was 1.4 times higher than that of the fumigated one.
  
  
  CONCLUSIONS
  After 100 d fumigating soil, the composition of nosZ denitrifying microbial community and the population of denitrifying bacteria changed. Furthermore, there was no difference in denitrification between the fumigated soil and the control.
authors.forename: Pingmei
authors.initials: P
authors.lastname: Yan
authors.forename: Hongping
authors.initials: H
authors.lastname: Qiao
authors.forename: Wenjing
authors.initials: W
authors.lastname: Zhao
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Chen
authors.forename: Shuhua
authors.initials: S
authors.lastname: Shan
authors.forename: Aocheng
authors.initials: A
authors.lastname: Cao
mesh_terms: D001419:Bacteria / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D044822:Biodiversity; D058440:Denitrification / Q000187:drug effects; D005651:Fumigation; D006842:Hydrocarbons, Brominated / Q000494:pharmacology*; D010088:Oxidoreductases / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D012988:Soil Microbiology
chemical_list: D001426:Bacterial Proteins; D006842:Hydrocarbons, Brominated; C005218:methyl bromide; D010088:Oxidoreductases; C022763:nitrous oxide reductase