title: Effects of miRNA-197 overexpression on proliferation, apoptosis and migration in levonorgestrel treated uterine leiomyoma cells.
abstract: OBJECTIVE
  Uterine leiomyoma is the ahead benign tumor of the female genital tract, which resulted in menstrual abnormalities, recurrent pregnancy loss, and other serious gynecological disorders in women. Recently, as the process of exploring the brief molecular mechanisms of tumorgenesis, microRNAs (miRNAs) have attracted much more attention.
  
  
  METHODS
  In this study, we first confirmed that microRNA-197 (miR-197) was down-regulated significantly in human uterus leiomyoma by quantity real-time polymerase chain reaction, compared to normal uterus myometrium. Then we observed the potential effects of miR-197 overexpression on human uterus leiomyoma cells by cell counting kit 8, wound healing assay, and flow cytometric assessment separately.
  
  
  RESULTS
  The data showed that miR-197 could inhibit cell proliferation, induce cell apoptosis, and block cell migration in vitro. Coincidently, levonorgestrel (LNG), a well-known uterus leiomyoma therapy, could induce miR-197 expression in human uterus leiomyoma cells, and over-expression of miR-197 showed a synergy effect on human uterus leiomyoma cell proliferation and apoptosis with LNG.
  
  
  CONCLUSIONS
  In this study, the data showed that miR-197 could play an anti-oncogenic role in human uterus leiomyoma cells, and cooperate with LNG on the cell proliferation and apoptosis, which suggested that miR-197 might be a potential target and provided database for clinical treatment.
authors.affiliation: Department of Women Health Care, Nanjing Maternal and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210004, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Obstetrics and Gynecology, Affiliated Jiangyin Hospital of South-East University, Jiangyin 214400, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ling
authors.affiliation: Nanjing Maternal and Child Health Medical Institute, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210004, China.
authors.forename: Ziyi
authors.initials: Z
authors.lastname: Fu
authors.affiliation: Nanjing Maternal and Child Health Medical Institute, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210004, China.
authors.forename: Chenbo
authors.initials: C
authors.lastname: Ji
authors.affiliation: Department of Women Health Care, Nanjing Maternal and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210004, China.
authors.forename: Jiangping
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Obstetrics and Gynecology, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210004, China. Electronic address: qingxunjmu@163.com.
authors.forename: Qing
authors.initials: Q
authors.lastname: Xu
mesh_terms: D000328:Adult; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D002465:Cell Movement / Q000187:drug effects* / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D015536:Down-Regulation / Q000187:drug effects; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D007889:Leiomyoma / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D016912:Levonorgestrel / Q000494:pharmacology* / Q000627:therapeutic use; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D014407:Tumor Cells, Cultured; D014594:Uterine Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology
chemical_list: C546085:MIRN197 microRNA, human; D035683:MicroRNAs; D016912:Levonorgestrel
keywords: Apoptosis; Cell proliferation; Levonorgestrel (LNG); Migration; Uterine leiomyoma; miRNA-197 overexpression