title: Influence of fetuin and hyaluronan on the post-thaw quality and fertilizing ability of Holstein bull semen.
abstract: It was determined that fetuin and hyaluronan supplementation did not provide any significant effect on the post-thaw subjective and CASA motility percentages and sperm motion characteristics, in comparison to the controls (P>0.05). Sperm acrosome and total abnormalities were similar in all groups (P>0.05). Groups M (hyaluronan+fetuin) and H (hyaluronan) displayed a higher rate of sperm membrane integrity, compared to that of Group C (control) (P<0.01). According to the results of the comet assay, the lowest percentage of sperm with damaged DNA was achieved in Group H, when compared to all of the experimental groups (P<0.01). Furthermore, all of the additives resulted in a lower rate of sperm with damaged DNA than that of the controls, and thus, reduced DNA damage (P<0.01). For pregnancy rates, there were no significant differences between the extender groups (P>0.05). MDA formation was found to be lower in Groups M and F (P<0.01). In Group M, SOD activity was determined to have significantly increased (23.61±5.62 U/ml) compared to the other groups (P<0.01). All experimental groups had a GSH-Px activity higher than that of the control group (P<0.01).
authors.affiliation: Erciyes University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, Kayseri, Turkey; Erciyes University, Genome and Stem Cell Center-GENKOK, Kayseri, Turkey. Electronic address: sariozkan75@yahoo.com.
authors.forename: Serpil
authors.initials: S
authors.lastname: Sarıözkan
authors.affiliation: Selcuk University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, Konya, Turkey. Electronic address: mnumanbucak@gmail.com.
authors.forename: Mustafa Numan
authors.initials: MN
authors.lastname: Bucak
authors.affiliation: Aksaray University, Vocational School, Aksaray, Turkey.
authors.forename: Pürhan Barbaros
authors.initials: PB
authors.lastname: Tuncer
authors.affiliation: Aksaray University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, Aksaray, Turkey.
authors.forename: Serhat
authors.initials: S
authors.lastname: Büyükleblebici
authors.affiliation: Erciyes University, Faculty of Pharmacy, Department of Pharmaceutical Toxicology, Kayseri, Turkey.
authors.forename: Ayşe
authors.initials: A
authors.lastname: Eken
authors.affiliation: GATA School of Medicine, Department of Pharmaceutical Sciences, Ankara, Turkey.
authors.forename: Cemal
authors.initials: C
authors.lastname: Akay
mesh_terms: D000177:Acrosome / Q000502:physiology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002417:Cattle; D020552:Comet Assay; D015925:Cryopreservation / Q000379:methods; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects; D005260:Female; D060748:Fetuins / Q000494:pharmacology*; D006801:Humans; D006820:Hyaluronic Acid / Q000494:pharmacology*; D008297:Male; D011247:Pregnancy; D012661:Semen / Q000502:physiology; D055101:Semen Analysis / Q000379:methods*; D012662:Semen Preservation / Q000379:methods*; D013081:Sperm Motility / Q000187:drug effects / Q000502:physiology
chemical_list: D000975:Antioxidants; D003451:Cryoprotective Agents; D060748:Fetuins; D006820:Hyaluronic Acid
keywords: Antioxidants; Biochemical parameters; Bull; DNA damage; Freeze-thawing; Sperm parameters