title: [Attenuated total reflection-fourier transform infrared spectroscopic study of dried shark fin products].
abstract: Sixty-four pieces of shark fin dried products (including real, fake and artificial shark fin products) and real products coated with gelatin were rapidly and nondestructively analyzed by attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR). The characteristic of IR spectrograms among the above four kinds of samples were systematically studied and comparied, the results showed that the spectrograms of the same kind of samples were repeatable, and different kinds of shark fin products presented significant differences in the spectrograms, which mainly manifested as the specific absorption peaks of amido bonds in protein (1650, 1544 cm(-1)) and skeletal vibration in polysaccharide (1050 cm(-1)). The spectrograms of real shark fins were characterized by the strong absorption peaks of protein characteristic amide I and II absorbent (1650, 1544 cm(-1)) and relatively weak C--O--C vibration absorbent (1050 cm(-1)) owing to the high content of protein and relatively low level of polysaccharide. For fake shark fin products that were molded form by mixing together with the offcut of shark, collagen and other substances, the introduction of non-protein materials leaded to the weaker amido bonds absorbent than real products along with a 30 cm(-1) blue shift of amide I absorbent. Opposite to the real sample, the relatively strong absorption peak of polysaccharide (approximately 1047 cm(-1)) and barely existed amide absorbent were the key features of the spectrogram of artificial samples, which was synthersized by polysaccharide like sodium alginate. Real samples coated with gelatin, the peak strength of protein and polysaccharide were decreased simultaneously when the data collection was taken at the surface of sample, while the spectrogram presented no significant difference to real samples when the data was collected in the section. The results above indicated that by analyzing the characteristic of IR spectrograms and the value range of Apro/Apol collected by ATR-FTIR method could perform the undamaged and rapid identification for shark fins.
authors.forename: Wan-qing
authors.initials: WQ
authors.lastname: Han
authors.forename: Hai-ying
authors.initials: HY
authors.lastname: Luo
authors.forename: Yan-ping
authors.initials: YP
authors.lastname: Xian
authors.forename: Dong-hui
authors.initials: DH
authors.lastname: Luo
authors.forename: Torng-na
authors.initials: TN
authors.lastname: Mu
authors.forename: Xin-dong
authors.initials: XD
authors.lastname: Guo
mesh_terms: D000464:Alginates; D058500:Animal Fins / Q000737:chemistry*; D000818:Animals; D003094:Collagen; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D011506:Proteins; D017747:Seafood / Q000032:analysis*; D012754:Sharks; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D011506:Proteins; D020723:Glucuronic Acid; D003094:Collagen