title: Selective label-free electrochemical impedance measurement of glycated haemoglobin on 3-aminophenylboronic acid-modified eggshell membranes.
abstract: We propose a novel alternative approach to long-term glycaemic monitoring using eggshell membranes (ESMs) as a new immobilising platform for the selective label-free electrochemical sensing of glycated haemoglobin (HbA1c), a vital clinical index of the glycaemic status in diabetic individuals. Due to the unique features of a novel 3-aminophenylboronic acid-modified ESM, selective binding was obtained via cis-diol interactions. This newly developed device provides clinical applicability as an affinity membrane-based biosensor for the identification of HbA1c over a clinically relevant range (2.3 - 14 %) with a detection limit of 0.19%. The proposed membrane-based biosensor also exhibited good reproducibility. When analysing normal and abnormal HbA1c levels, the within-run coefficients of variation were 1.68 and 1.83%, respectively. The run-to-run coefficients of variation were 1.97 and 2.02%, respectively. These results demonstrated that this method achieved the precise and selective measurement of HbA1c. Compared with a commercial HbA1c kit, the results demonstrated excellent agreement between the techniques (n = 15), demonstrating the clinical applicability of this sensor for monitoring glycaemic control. Thus, this low-cost sensing platform using the proposed membrane-based biosensor is ideal for point-of-care diagnostics.
authors.affiliation: Graduate Program in Clinical Biochemistry and Molecular Medicine, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, 10330, Thailand.
authors.forename: Yuwadee
authors.initials: Y
authors.lastname: Boonyasit
authors.forename: Arto
authors.initials: A
authors.lastname: Heiskanen
authors.forename: Orawan
authors.initials: O
authors.lastname: Chailapakul
authors.forename: Wanida
authors.initials: W
authors.lastname: Laiwattanapaisal
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation; D001897:Boronic Acids / Q000737:chemistry*; D002645:Chickens; D058266:Dielectric Spectroscopy / Q000295:instrumentation*; D004528:Egg Shell / Q000737:chemistry*; D017097:Electric Impedance; D004867:Equipment Design; D006442:Glycated Hemoglobin / Q000032:analysis*; D006801:Humans; D057230:Limit of Detection; D008566:Membranes / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D001897:Boronic Acids; D006442:Glycated Hemoglobin; C028592:3-aminobenzeneboronic acid