title: Effects of p-chlorophenoxyisobutyric acid, arabinogalactan, and activated charcoal on microspore embryogenesis in kale.
abstract: To improve embryogenesis in microspore cultures of kale (Brassica oleracea L. var. acephala DC.), 6-benzylaminopurine (6-BA), naphthaleneacetic acid (NAA), arabinogalactan (AG), p-chlorophenoxyisobutyric acid (PCIB), and activated charcoal (AC) were added to the medium using four varieties of kale. The results showed that the addition of AG (0.1-0.2 g/L), AC (0.1-0.2 g/L) or a combination of 6-BA (0.1-0.2 mg/L) and NAA (0.1-0.2 mg/L) promoted embryo-genesis. Adding 40 μM PCIB or a combination of 40 μM PCIB and 0.2 g/L AC to NLN-13 medium at pH 5.8 effectively enhanced embryogenesis. Treatment with a combination of 40 μM PCIB and 10 mg/L AG gave the highest rate of embryonic induction, especially in genotype "Y007," which showed a twelve-fold increase in yield.
authors.affiliation: College of Horticulture, Shenyang Agricultural University, Shenyang, China.
authors.forename: R Q
authors.initials: RQ
authors.lastname: Niu
authors.affiliation: College of Horticulture, Shenyang Agricultural University, Shenyang, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Horticulture, Shenyang Agricultural University, Shenyang, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Tong
authors.affiliation: College of Horticulture, Shenyang Agricultural University, Shenyang, China.
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Liu
authors.affiliation: College of Horticulture, Shenyang Agricultural University, Shenyang, China.
authors.forename: Y H
authors.initials: YH
authors.lastname: Wang
authors.affiliation: College of Horticulture, Shenyang Agricultural University, Shenyang, China fenghuiaaa@263.net.
authors.forename: H
authors.initials: H
authors.lastname: Feng
mesh_terms: D001937:Brassica / Q000196:embryology* / Q000235:genetics; D002606:Charcoal / Q000494:pharmacology*; D002995:Clofibric Acid / Q000494:pharmacology*; D005685:Galactans / Q000494:pharmacology*; D011003:Ploidies; D012639:Seeds / Q000254:growth & development*
chemical_list: D005685:Galactans; D002606:Charcoal; D002995:Clofibric Acid; C005653:arabinogalactan