title: Citrinin Determination in Red Fermented Rice Products by Optimized Extraction Method Coupled to Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS).
abstract: A rapid and sensitive method was developed and validated for citrinin determination in red fermented rice products by liquid chromatography tandem mass spectrometry (LC-MS/MS) under the selected reaction monitoring mode. Sample preparation was especially focused, and the quantitative methods of LC-MS/MS and high-performance liquid chromatography with fluorescence detection (HPLC-FLD) were compared. In red fermented rice samples, the limit of detection was 1.0 μg/kg for LC-MS/MS compared to 250 μg/kg for HPLC-FLD, the limit of quantification was 3.0 μg/kg for LC-MS/MS compared to 825 μg/kg for HPLC-FLD. High correlation coefficient was obtained (R(2) = 0.999) within the linear range (0.1 to 100 μg/L) in the MS method. The recoveries ranging from 80.9% to 106.5% were obtained in different spiking concentrations. The average intra- and inter-day accuracy ranged from 75.4% to 103.1%, and the intra- and inter-day precisions were from 3.3% to 7.9%. The developed method was applied to 12 commercial red fermented rice products, and citrinin was found in 10 samples ranging from 0.14 to 44.24 mg/kg. Compared to traditional qualitative and quantitative methods, the newly developed LC-MS/MS method for citrinin determination includes the merits of using a small amount of extraction solvent, simple preparation steps, and high sensitivity.
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Plant Pest Control, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, P.R., China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Ji
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Plant Pest Control, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, P.R., China.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Xu
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Plant Pest Control, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, P.R., China.
authors.forename: Xiaofu
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Plant Pest Control, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, P.R., China.
authors.forename: Peipei
authors.initials: P
authors.lastname: Qi
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Plant Pest Control, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, P.R., China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Plant Pest Control, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, P.R., China.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Chen
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Plant Pest Control, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, P.R., China.
authors.forename: Rui
authors.initials: R
authors.lastname: Li
authors.affiliation: School of Tea and Food Science Technology, Anhui Agricultural Univ., Hefei, 230036, P.R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D019149:Bioreactors; D002851:Chromatography, High Pressure Liquid; D002953:Citrinin / Q000032:analysis*; D005285:Fermentation; D057230:Limit of Detection; D012275:Oryza / Q000737:chemistry*; D015203:Reproducibility of Results; D018570:Risk Assessment; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry
chemical_list: D002953:Citrinin
keywords: HPLC-FLD; LC-MS/MS; citrinin; mycotoxin; red fermented rice