title: Ectopic osteogenic tissue formation by MC3T3-E1 cell-laden chitosan/hydroxyapatite composite scaffold.
abstract: This study evaluates the suitability of a macroporous three-dimensional chitosan/hydroxyapatite (CS/HA) composite as a bone tissue engineering scaffold using MC3T3-E1 cells. The CS/HA scaffold was produced by freeze-drying, and characterized by means of SEM and FTIR. In vitro findings demonstrated that CS/HA supported attachment and proliferation of cells, and stimulated extracellular matrix (ECM) production. Tissue biocompatibility and osteogenic capacity of the cell-laden constructs were evaluated in an ectopic Wistar rat model. In vivo results showed that the MC3T3-E1 cell-laden CS/HA was essentially histocompatible, promoted neovascularization and calcified matrix formation, and secreted osteoblast-specific protein. We conclude that the composite scaffold evaluated has potential for applications in bone regeneration.
authors.affiliation: a Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory, Ankara University Faculty of Science, and Ankara University Stem Cell Institute , Ankara , Turkey.
authors.forename: Aysel
authors.initials: A
authors.lastname: Koç
authors.affiliation: a Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory, Ankara University Faculty of Science, and Ankara University Stem Cell Institute , Ankara , Turkey.
authors.forename: Ayşe Eser
authors.initials: AE
authors.lastname: Elçin
authors.affiliation: a Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory, Ankara University Faculty of Science, and Ankara University Stem Cell Institute , Ankara , Turkey.
authors.forename: Yaşar Murat
authors.initials: YM
authors.lastname: Elçin
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D010012:Osteogenesis / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D048271:Chitosan; D017886:Durapatite
keywords: biocompatibility; bone tissue engineering; chitosan/hydroxyapatite; composite scaffold; ectopic bone formation; preosteoblast; xenotransplantation