title: The changes in miR-130b levels in human serum and the correlation with the severity of diabetic nephropathy.
abstract: BACKGROUND
  Circulating microRNA 130b has been closely associated with multiple diseases in humans such as cancer, obesity and diabetes mellitus. This study evaluates the correlation between serum miR-130b and the severity of diabetic nephropathy evaluated by measurement of albuminuria.
  
  
  METHODS
  Three hundred twenty-seven patients with type 2 diabetes mellitus (T2DM) were divided into three groups: normoalbuminuria group [diabetes mellitus, urinary albumin to creatinine ratio (UACR) < 30 mg/g, n = 137], microalbuminuria group (DN1, UACR 30-300 mg/g, n = 122) and macroalbuminuria group (DN2, UACR > 300 mg/g, n = 68). The levels of serum miR-130b were validated by real-time polymerase chain reaction. Serum transforming growth factor β1 (TGF-β1), hypoxia inducible factor 1α (HIF-1α) and fibronectin were determined by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  Compared with control, serum miR-130b levels were significantly decreased in T2DM patients and further decreased in the patients of diabetes mellitus, DN1 and DN2 groups (p < 0.001). Furthermore, age-adjusted and sex-adjusted regression analyses showed that decreased level of serum miR-130b, increased levels of glycated haemoglobin (HbA1c ), homeostatic model assessment of insulin resistance (HOMA-IR), triglyceride (TG), low-density lipoprotein (LDL), serum creatinine, blood urea nitrogen (BUN), TGF-β1, HIF-1α and fibronectin were significantly correlated with UACR (p < 0.05). In addition, serum miR-130b levels were inversely correlated with HbA1c , HOMA-IR, TG, LDL, BUN, TGF-β1, HIF-1α and FN (p < 0.05).
  
  
  CONCLUSIONS
  Our findings suggest that serum miR-130b may be a new biomarker for the early diagnosis of DN in T2DM. Circulating miR-130b may possibly be involved in the pathological mechanism of DN, such as lipid metabolic disorders, oxidative stress, extracellular matrix deposition and renal fibrosis.
authors.affiliation: Department of Endocrinology, the First Hospital Affiliated to China Medical University, Shenyang, Liaoning, 110001, China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Lv
authors.affiliation: Division of Endocrinology, Shenyang No. 8 Hospital, Shenyang, Liaoning, 110024, China.
authors.forename: Yue-Hong
authors.initials: YH
authors.lastname: Zhou
authors.affiliation: Department of Endocrinology, the First Hospital Affiliated to China Medical University, Shenyang, Liaoning, 110001, China.
authors.forename: Can
authors.initials: C
authors.lastname: Wu
authors.affiliation: Department of Endocrinology, the First Hospital Affiliated to China Medical University, Shenyang, Liaoning, 110001, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Shao
authors.affiliation: Department of Endocrinology, the First Hospital Affiliated to China Medical University, Shenyang, Liaoning, 110001, China.
authors.forename: Can-Lu
authors.initials: CL
authors.lastname: Lu
authors.affiliation: Department of Endocrinology, the First Hospital Affiliated to China Medical University, Shenyang, Liaoning, 110001, China.
authors.forename: Qiu-Yue
authors.initials: QY
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D000419:Albuminuria / Q000097:blood* / Q000209:etiology; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D003430:Cross-Sectional Studies; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000150:complications; D003928:Diabetic Nephropathies / Q000097:blood* / Q000209:etiology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005353:Fibronectins / Q000097:blood*; D006442:Glycated Hemoglobin / Q000378:metabolism; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000097:blood*; D008077:Lipoproteins, LDL / Q000097:blood; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D012720:Severity of Illness Index; D053773:Transforming Growth Factor beta1 / Q000097:blood*; D014280:Triglycerides / Q000097:blood
chemical_list: D003404:Creatinine; D005353:Fibronectins; D006442:Glycated Hemoglobin; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D008077:Lipoproteins, LDL; D035683:MicroRNAs; D053773:Transforming Growth Factor beta1; D014280:Triglycerides; C497442:HIF1A protein, human; C554019:MIRN130 microRNA, human; C508960:TGFB1 protein, human; C517652:hemoglobin A1c protein, human
keywords: albuminuria; circulating miR-130b; diabetic nephropathy; type 2 diabetes mellitus