title: Decellularized porcine aortic intima-media as a potential cardiovascular biomaterial.
abstract: OBJECTIVE
  The aim of this research is to investigate the histological and mechanical properties of decellularized aortic intima-media, a promising cardiovascular biomaterial.
  
  
  METHODS
  Porcine aortic intima-media was decellularized using two methods: high hydrostatic pressurization (HHP) and sodium dodecyl sulphate (SDS). The histological properties were characterized using haematoxylin and eosin staining and Elastica van Gieson staining. The mechanical properties were evaluated using a tensile strength test.
  
  
  RESULTS
  The structure of the HHP-treated samples was unchanged histologically, whereas that of the SDS-treated samples appeared structurally loose. Consequently, with regard to the mechanical properties of SDS-decellularized intima-media, elastic modulus and tensile strength were significantly decreased.
  
  
  CONCLUSIONS
  The decellularization method affected the structure and the mechanical properties of the biomaterial. The HHP-treated sample was structurally and mechanically similar to the untreated control. Its mechanical properties were similar to those of human heart valves and the iliac artery and vein. Our results imply that porcine aortic intima-media that is decellularized with HHP is a potential cardiovascular biomaterial.
authors.affiliation: Department of Material-Based Medical Engineering, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Pingli
authors.initials: P
authors.lastname: Wu
authors.affiliation: Department of Material-Based Medical Engineering, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Nakamura
authors.affiliation: Department of Material-Based Medical Engineering, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Kimura
authors.affiliation: Department of Material-Based Medical Engineering, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Kwangwoo
authors.initials: K
authors.lastname: Nam
authors.affiliation: Department of Biomedical Engineering, Osaka Institute of Technology, Osaka, Japan.
authors.forename: Toshiya
authors.initials: T
authors.lastname: Fujisato
authors.affiliation: Department of Cardiovascular Surgery, Sapporo Medical University School of Medicine, Sapporo, Japan.
authors.forename: Seiichi
authors.initials: S
authors.lastname: Funamoto
authors.affiliation: Department of Cardiovascular Surgery, Sapporo Medical University School of Medicine, Sapporo, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Higami
authors.affiliation: Department of Material-Based Medical Engineering, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, Japan kishida.mbme@tmd.ac.jp.
authors.forename: Akio
authors.initials: A
authors.lastname: Kishida
mesh_terms: D000818:Animals; D001011:Aorta / Q000502:physiology* / Q000637:transplantation; D001672:Biocompatible Materials; D001696:Biomechanical Phenomena; D001705:Bioprosthesis; D002318:Cardiovascular Diseases / Q000601:surgery; D006350:Heart Valve Prosthesis; D006801:Humans; D011474:Prosthesis Design; D013552:Swine; D023822:Tissue Engineering; D017539:Tunica Intima / Q000502:physiology / Q000637:transplantation; D017540:Tunica Media / Q000502:physiology / Q000637:transplantation
chemical_list: D001672:Biocompatible Materials
keywords: Cardiovascular biomaterial; Decellularized aortic intima-media; Mechanical properties; Tissue structure