title: Virus safety of islet cell transplantation from transgenic pigs to marmosets.
abstract: Transplantation of pig islet cells for the treatment of diabetes may be a more effective approach compared with the application of insulin. However, before introduction into the clinic, efficacy and safety of this treatment have to be shown. Non-human primate models may be used for this, despite the fact that they are characterised by several limitations. Here we investigate the prevalence of porcine endogenous retroviruses (PERVs), which are present in the genome of all pigs and which may infect human cells, as well as of porcine herpes viruses in donor pigs and their potential transmission to non-human primate recipients. Despite the fact that all three subtypes of PERV were present in all and porcine cytomegalovirus (PCMV) was found in some of the pigs, neither PERVs nor PCMV were found in the recipient animals under the experimental conditions applied. Porcine lymphotropic herpes viruses (PLHV) were not found in the donor pigs, hepatitis E virus (HEV) was not found in the recipients.
authors.affiliation: Robert Koch Institute, HIV and Other Retroviruses, Nordufer 20, 13353 Berlin, Germany. Electronic address: PlotzkiE@rki.de.
authors.forename: Elena
authors.initials: E
authors.lastname: Plotzki
authors.affiliation: Medizinische Klinik und Poliklinik IV, Diabeteszentrum, Ludwig-Maximilians-Universität, München, Ziemssenstraße 1, 80336 München, Germany. Electronic address: Lelia.van_Buerck@med.uni-muenchen.de.
authors.forename: Lelia
authors.initials: L
authors.lastname: Wolf-van Buerck
authors.affiliation: German Primate Center, Leibniz-Institute, Pathology Unit, Kellnerweg 4, 37077 Göttingen, Germany. Electronic address: YKnauf@dpz.eu.
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Knauf
authors.affiliation: German Primate Center, Leibniz-Institute, Pathology Unit, Kellnerweg 4, 37077 Göttingen, Germany. Electronic address: tbecker@dpz.eu.
authors.forename: Tamara
authors.initials: T
authors.lastname: Becker
authors.affiliation: German Primate Center, Leibniz-Institute, Pathology Unit, Kellnerweg 4, 37077 Göttingen, Germany. Electronic address: kmaetz@dpz.eu.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Maetz-Rensing
authors.affiliation: Medizinische Klinik und Poliklinik IV, Diabeteszentrum, Ludwig-Maximilians-Universität, München, Ziemssenstraße 1, 80336 München, Germany. Electronic address: Marion.Schuster@med.uni-muenchen.de.
authors.forename: Marion
authors.initials: M
authors.lastname: Schuster
authors.affiliation: Chair for Molecular Animal Breeding and Biotechnology and Laboratory for Functional Genome Analysis (LAFUGA), Gene Center, Ludwig-Maximilians-Universität München, Hackerstraße 27, 85764 Oberschleißheim, Germany. Electronic address: A.Baehr@gen.vetmed.uni-muenchen.de.
authors.forename: Andrea
authors.initials: A
authors.lastname: Baehr
authors.affiliation: Chair for Molecular Animal Breeding and Biotechnology and Laboratory for Functional Genome Analysis (LAFUGA), Gene Center, Ludwig-Maximilians-Universität München, Hackerstraße 27, 85764 Oberschleißheim, Germany. Electronic address: N.Klymiuk@gen.vetmed.uni-muenchen.de.
authors.forename: Nikolai
authors.initials: N
authors.lastname: Klymiuk
authors.affiliation: Chair for Molecular Animal Breeding and Biotechnology and Laboratory for Functional Genome Analysis (LAFUGA), Gene Center, Ludwig-Maximilians-Universität München, Hackerstraße 27, 85764 Oberschleißheim, Germany. Electronic address: ewolf@lmb.uni-muenchen.de.
authors.forename: Eckhard
authors.initials: E
authors.lastname: Wolf
authors.affiliation: Medizinische Klinik und Poliklinik IV, Diabeteszentrum, Ludwig-Maximilians-Universität, München, Ziemssenstraße 1, 80336 München, Germany. Electronic address: Jochen.Seissler@med.uni-muenchen.de.
authors.forename: Jochen
authors.initials: J
authors.lastname: Seissler
authors.affiliation: Robert Koch Institute, HIV and Other Retroviruses, Nordufer 20, 13353 Berlin, Germany. Electronic address: DennerJ@rki.de.
authors.forename: Joachim
authors.initials: J
authors.lastname: Denner
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002144:Callithrix; D003920:Diabetes Mellitus / Q000601:surgery*; D020077:Endogenous Retroviruses / Q000502:physiology*; D005260:Female; D006564:Herpesviridae / Q000502:physiology*; D006566:Herpesviridae Infections / Q000635:transmission*; D006801:Humans; D007515:Islets of Langerhans / Q000821:virology; D016381:Islets of Langerhans Transplantation / Q000009:adverse effects*; D008297:Male; D012192:Retroviridae Infections / Q000635:transmission*; D012449:Safety; D013552:Swine
keywords: Porcine endogenous retroviruses; Porcine hepatitis E virus; Porcine herpes viruses; Virus safety; Xenotransplantation