title: Cranberries (Oxycoccus quadripetalus) inhibit lipid metabolism and modulate leptin and adiponectin secretion in 3T3-L1 adipocytes.
abstract: It has previously been shown that lyophilized cranberries (LCB) decreased lipid accumulation in 3T3-L1 cells and inhibited preadipocyte differentiation by down-regulation of the expression of key transcription factors (PPARγ, C/EBPα, SREBP1) of the adipogenesis pathway. To elucidate the molecular basis of anti-lipogenic activity of LCB, the expression of several genes involved in lipid metabolism, such as adipocyte fatty acid-binding protein (aP2), lipoprotein lipase (LPL), fatty acid synthase (FAS), hormone sensitive lipase (HSL) and perilipin 1 (PLIN1), was examined in the present study. Additionally, the effects of LCB on adiponectin and leptin expression and protein secretion were also investigated. LCB reduced lipid accumulation during preadipocyte differentiation by down-regulation of the mRNA level of aP2, FAS, LPL, HSL and PLIN1. Moreover, LCB decreased leptin gene expression and increased adiponectin gene expression and protein secretion in a dose-dependent manner. Therefore cranberries could be considered as bioactive factors, which are effective in the inhibition of adipose tissue mass production.
authors.affiliation: Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Wojska Polskiego 48 Str., 60-627 Poznan, Poland. Electronic address: kaskakow@up.poznan.pl.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kowalska
authors.affiliation: Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Wojska Polskiego 48 Str., 60-627 Poznan, Poland. Electronic address: aolejnik@up.poznan.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Olejnik
authors.affiliation: Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Wojska Polskiego 48 Str., 60-627 Poznan, Poland. Electronic address: joanna.rychlik@up.poznan.pl.
authors.forename: Joanna
authors.initials: J
authors.lastname: Rychlik
authors.affiliation: Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Wojska Polskiego 48 Str., 60-627 Poznan, Poland. Electronic address: grajek@up.poznan.pl.
authors.forename: Włodzimierz
authors.initials: W
authors.lastname: Grajek
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism; D052242:Adiponectin / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D020738:Leptin / Q000235:genetics / Q000378:metabolism*; D050356:Lipid Metabolism / Q000187:drug effects*; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D029799:Vaccinium macrocarpon
chemical_list: D052242:Adiponectin; C499919:Adipoq protein, mouse; D020738:Leptin; D010936:Plant Extracts; D012333:RNA, Messenger
keywords: 3T3-L1 cells; Adiponectin; Cranberries; Leptin; Lipogenesis