title: Efficacy of optimized in vitro predegeneration period on the cell count and purity of canine Schwann cell cultures.
abstract: OBJECTIVE
  Predegeneration is a standard technique to obtain mitotically activated and enriched cultures of Schwann cells (SCs). This study, for the first time, evaluated the impact of various duration of predegeneration on cell yield and enrichment of SCs from dog peripheral nerve.
  
  
  METHODS
  Dog sural nerves were subjected to 5, 10, 15 day-long in vitro predegeneration. The total cell yield and the purity of SCs were evaluated in each group on the first and seventh day after plating.
  
  
  RESULTS
  The maximum and minimum numbers of cells were counted in 15 day-long predegene-ration and control groups which underwent no predegeneration. The 10 day-long in vitro predegeneration group with 80±0.5% SCs enrichment had the best purity after plating day and could maintain its purity with elapsing on cultures.
  
  
  CONCLUSIONS
  10 day-long predegeneration results in the higher cell number and the better and prolonged purity of SCs in culture.
authors.affiliation: Department of Anatomical Sciences, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
authors.forename: Nazila
authors.initials: N
authors.lastname: Niapour
authors.affiliation: Department of Microbiology and Parasitology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
authors.forename: Behnam
authors.initials: B
authors.lastname: Mohammadi-Ghalehbin
authors.affiliation: Department of Anatomical Sciences, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
authors.forename: Mohammad Ghasem
authors.initials: MG
authors.lastname: Golmohammadi
authors.affiliation: Department of Physiology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Amani
authors.affiliation: Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Salehi
authors.affiliation: Department of Anatomical Sciences, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Niapour
keywords: Dog; Duration; Schwann cell; Sural nerve; Wallerian degeneration