title: Role of intracellular free calcium in killing Penicillium marneffei within human macrophages.
abstract: Increases in cytosolic Ca(2+) concentration ([Ca(2+)]c) promote phagocyte antimicrobial responses. Here, we investigated macrophages stimulated by Penicillium marneffei (P. marneffei). [Ca(2+)]c was determined in macrophages loaded with the fluorescent calcium probe Fura 2/AM as they were stimulated by P. marneffei. We found that P. marneffei induced an increase in [Ca(2+)]c in human macrophages. Further, increased [Ca(2+)]c with the ionophore A23187 promoted phagosomal acidification and maturation and reduced intracellular replication of P. marneffei in P. marneffei-infected human macrophages, whereas decreased [Ca(2+)]c with the chelation MAPTAM decreased TNF-α production, inhibited phagosomal acidification and maturation and increased intracellular replication of P. marneffei. These data indicate that Ca(2+) signaling may play an important role in controlling the replication of P. marneffei within macrophages.
authors.affiliation: Department of Dermatology, Lianyungang First People's Hospital, Lianyungang 222002, China.
authors.forename: Renqiong
authors.initials: R
authors.lastname: Chen
authors.affiliation: Department of Dermatology, Lianyungang First People's Hospital, Lianyungang 222002, China.
authors.forename: Guangquan
authors.initials: G
authors.lastname: Ji
authors.affiliation: Department of Dermatology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Tuan
authors.initials: T
authors.lastname: Ma
authors.affiliation: Department of Dermatology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Huang
authors.affiliation: Department of Dermatology, Lianyungang First People's Hospital, Lianyungang 222002, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Ren
authors.affiliation: Department of Dermatology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China. Electronic address: xiliyan718@163.com.
authors.forename: Liyan
authors.initials: L
authors.lastname: Xi
mesh_terms: D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D003600:Cytosol / Q000737:chemistry; D006801:Humans; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology*; D050296:Microbial Viability; D010407:Penicillium / Q000187:drug effects / Q000276:immunology* / Q000502:physiology*; D010588:Phagosomes / Q000276:immunology / Q000378:metabolism / Q000382:microbiology
chemical_list: D002118:Calcium
keywords: Calcium; Macrophages; Penicillium marneffei; TNF-α