title: Healthy twin live-birth after ionophore treatment in a case of theophylline-resistant Kartagener syndrome.
abstract: OBJECTIVE
  To evaluate whether it is a feasible option to target the oocyte (with Ca(2+)-ionophore) in case that sperm motility cannot be restored in Kartagener syndrome.
  
  
  METHODS
  A case of a male Kartagener syndrome with exclusively immotile spermatozoa that did not react to the dimethylxanthine theophylline. Thus, half of the associated oocytes were treated for 15 min with the ready-to-use- ionophore CultActive immediately after ICSI whereas the other 50 % were injected with routine ICSI without artificial oocyte activation. Rates of fertilization, blastulation, pregnancy and live birth were evaluated.
  
  
  RESULTS
  Fertilization check revealed that none of the conventionally injected but 4/6 (66.7 %) of the artificially activated oocytes showed two pronuclei. Three embryos were of good and one of fair quality. Corresponding blastocyst formation rate was 3 out of 4 (75 %). A double embryo transfer led to a healthy twin birth in the 34th week of gestation (two boys with a birth weight of 1724 g and 2199 g).
  
  
  CONCLUSIONS
  This case indicates that Ca(2+)-ionophore treatment in cycles from theophylline-resistant Kartagener syndrome patients is a feasible option. The future will show if routine application of A23187 in Kartagener or primary cilia dyskinesis patients will be of benefit.
authors.affiliation: Landes- Frauen- und Kinderklinik, Kinderwunsch Zentrum, Krankenhausstr. 26-30, A-4040, Linz, Upper Austria, Austria, Thomas.ebner@gespag.at.
authors.forename: T
authors.initials: T
authors.lastname: Ebner
authors.forename: M
authors.initials: M
authors.lastname: Maurer
authors.forename: P
authors.initials: P
authors.lastname: Oppelt
authors.forename: R B
authors.initials: RB
authors.lastname: Mayer
authors.forename: H C
authors.initials: HC
authors.lastname: Duba
authors.forename: W
authors.initials: W
authors.lastname: Costamoling
authors.forename: O
authors.initials: O
authors.lastname: Shebl
mesh_terms: D000328:Adult; D061207:Calcium Ionophores / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007248:Infertility, Male / Q000235:genetics / Q000628:therapy*; D007619:Kartagener Syndrome / Q000150:complications* / Q000235:genetics; D050498:Live Birth; D008297:Male; D011247:Pregnancy; D059285:Pregnancy, Twin; D020554:Sperm Injections, Intracytoplasmic; D013081:Sperm Motility / Q000187:drug effects; D013806:Theophylline / Q000494:pharmacology
chemical_list: D061207:Calcium Ionophores; D013806:Theophylline