title: Adoptive transfer of transforming growth factor-ß1-induced CD4+CD25+ regulatory T cells prevents immune response-mediated spontaneous abortion.
abstract: The effects of adoptive transfer of transforming growth factor (TGF)-ß1-induced regulatory T (Treg) cells in preventing spontaneous abortion in mice were investigated. CD4+CD25- cells were isolated from the spleens of pregnant CBA/J mice and induced into Treg cells positive for CD4, CD25 and forkhead box P3 (FOXP3) ex vivo using interleukin (IL)-2 and TGF-ß1. CBA/J mice were mated with DBA/2J mice to establish a model of spontaneous abortion and, on the first day of pregnancy, mice were injected intravenously with 2 × 105 either freshly isolated Treg cells or those induced with TGF-ß1. After 14 days, the surviving and reabsorbed fetuses in both groups were counted, and serum cytokine concentrations were measured by ELISA. Adoptive transfer of CD4+CD25+ or TGF-ß1-induced Treg cells significantly reduced the fetal resorption rate, increased serum IL-10 and TGF-ß1 concentrations and decreased interferon-? levels. In conclusion, the results of the present study indicate that adoptive transfer of TGF-ß1-induced Treg cells prevents spontaneous abortion in mice by increasing the secretion of T helper (Th) 2 cytokines and decreasing the secretion of Th1 cytokines.
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Qiu
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, China.
authors.forename: Yincheng
authors.initials: Y
authors.lastname: Teng
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai Jiao Tong University Affiliated International Peace Maternity and Child Health Hospital, 910 Hengshan Road, Shanghai 200030, China.
authors.forename: Yudong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai Jiao Tong University Affiliated International Peace Maternity and Child Health Hospital, 910 Hengshan Road, Shanghai 200030, China.|Corresponding author. Email: xuliang19770323@126.com.
authors.forename: Liang
authors.initials: L
authors.lastname: Xu
keywords: forkhead box P3 (FOXP3).