title: Melanocortinergic circuits from medial vestibular nuclei to the kidney defined by transneuronal transport of pseudorabies virus.
abstract: This study was designed to assess whether MC4R signaling existed in vestibular nuclei modulated the activity of kidney by a virally mediated transsynaptic tracing study. Pseudorabies virus (PRV)-614 was injected into the kidney in adult male MC4R-green fluorescent protein (GFP) transgenic mice (n = 5). After a survival time of 5 days, the mice were assigned to humanely sacrifice, and the brainstem were removed and sectioned, and processed for PRV-614 visualization. The neurochemical phenotype of MC4R-GFP-positive neurons was identified using fluorescence immunocytochemical labeling. PRV-614/MC4R-GFP dual labeled neurons were detected in medial vestibular nuclei. Our findings support the hypothesis that there exist melanocortinergic circuits from medial vestibular nuclei to the kidney.
authors.affiliation: Department of Vascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology University No. 1277 Jiefang Ave, Wuhan 430022, People's Republic of China.
authors.forename: Dan
authors.initials: D
authors.lastname: Shang
authors.affiliation: Hepatobiliary Surgery Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology University No. 1277 Jiefang Ave, Wuhan 430022, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xiong
authors.affiliation: Department of Anesthesiology and Pain Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology No. 1095 Jiefang Road, Wuhan 430030, People's Republic of China.
authors.forename: Hong-Bing
authors.initials: HB
authors.lastname: Xiang
authors.affiliation: Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology No. 1095 Jiefang Road, Wuhan 430030, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Hao
authors.affiliation: Department of Anesthesiology and Pain Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology No. 1095 Jiefang Road, Wuhan 430030, People's Republic of China.
authors.forename: Jiu-Hong
authors.initials: JH
authors.lastname: Liu
mesh_terms: D000818:Animals; D049452:Green Fluorescent Proteins / Q000378:metabolism; D011558:Herpesvirus 1, Suid; D007668:Kidney / Q000378:metabolism*; D008297:Male; D053486:Melanocortins / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D009434:Neural Pathways / Q000378:metabolism; D056325:Neuronal Tract-Tracers; D009474:Neurons / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism*; D014726:Vestibular Nuclei / Q000378:metabolism*
chemical_list: D053486:Melanocortins; D056325:Neuronal Tract-Tracers; D049452:Green Fluorescent Proteins
keywords: Melanocortin-4 receptor; Vestibular nuclei; kidney; pseudorabies virus; transsynaptic tracing