title: High-risk HPV detection and genotyping by APTIMA HPV using cervical samples.
abstract: High-risk human papillomavirus (HPV) detection and genotyping is critical for cervical cancer screening. Testing of 967 cervical cytology specimens in PreservCyt preservative revealed similar positivity rates for HC2 (13.8%) and APTIMA HPV (AHPV) tests (13.5%, p=0.89) and high overall agreement (94.6%, κ=0.77). A trend towards higher HPV16 positivity rates by the Cobas HPV test (23.0%, 26/113) compared to the AHPV genotyping assay (19.5%, 22/113; p=0.125) was noted. No cross-contamination was detected with AHPV in a challenge experiment.
authors.affiliation: ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT 84108, USA.
authors.forename: Michael T
authors.initials: MT
authors.lastname: Pyne
authors.affiliation: Department of Pathology, University of Utah School of Medicine, 15 N Medical Drive East, Salt Lake City, UT 84112, USA.
authors.forename: Camille L
authors.initials: CL
authors.lastname: Hamula
authors.affiliation: ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT 84108, USA.
authors.forename: Keith
authors.initials: K
authors.lastname: Tardif
authors.affiliation: ARUP Laboratories, 500 Chipeta Way, Salt Lake City, UT 84108, USA.
authors.forename: Christian
authors.initials: C
authors.lastname: Law
authors.affiliation: ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT 84108, USA; ARUP Laboratories, 500 Chipeta Way, Salt Lake City, UT 84108, USA. Electronic address: robert.schlaberg@path.utah.edu.
authors.forename: Robert
authors.initials: R
authors.lastname: Schlaberg
mesh_terms: D000328:Adult; D000368:Aged; D002584:Cervix Uteri / Q000821:virology*; D005260:Female; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000821:virology*; D014773:Virology / Q000379:methods
keywords: HPV