title: [Comparative analysis of natural and synthetic antimutagens as regulators of gene expression in human cells under exposure to ionizing radiation].
abstract: This paper studies the effect of plant peptides of thionine Ns-W2 extracted from seeds of fennel flower (Nigella sativa) and β-purothionine from wheat germs (Triticum kiharae), as well as a synthetic antimutagen (crown-compound), on the expression of several genes involved in the.control of cellular homeostasis, processes of carcinogenesis, and radiation response in human rhabdomyosarcoma cells (RD cells), T-lymphoblastoid cell line Jurkat, and blood cells. All of these agents acted as antimutagens-anticarcinogens, reducing the expression of genes involved in carcinogenesis (genes of families MMP, TIMP, and IAP and G-protein genes) in a tumor cell. A pronounced reduction in the mRNA level of these genes was caused by thionine Ns-W2, and the least effect was demonstrated by β-purothionine. Antimutagens had very little effect on the mRNA levels of the several studied genes in normal blood cells.
authors.forename: V F
authors.initials: VF
authors.lastname: Mikhailov
authors.forename: A A
authors.initials: AA
authors.lastname: Shishkina
authors.forename: I M
authors.initials: IM
authors.lastname: Vasilyeva
authors.forename: L V
authors.initials: LV
authors.lastname: Shulenina
authors.forename: N F
authors.initials: NF
authors.lastname: Raeva
authors.forename: E A
authors.initials: EA
authors.lastname: Rogozhin
authors.forename: M I
authors.initials: MI
authors.lastname: Startsev
authors.forename: G D
authors.initials: GD
authors.lastname: Zasukhina
authors.forename: S P
authors.initials: SP
authors.lastname: Gromov
authors.forename: M V
authors.initials: MV
authors.lastname: Alfimov
mesh_terms: D016587:Antimutagenic Agents / Q000008:administration & dosage* / Q000737:chemistry; D063646:Carcinogenesis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D031881:Nigella sativa / Q000737:chemistry; D010455:Peptides / Q000008:administration & dosage* / Q000737:chemistry; D010640:Phenothiazines / Q000008:administration & dosage* / Q000737:chemistry; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D011839:Radiation, Ionizing; D012208:Rhabdomyosarcoma / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D014908:Triticum / Q000737:chemistry; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis
chemical_list: D016587:Antimutagenic Agents; D010455:Peptides; D010640:Phenothiazines; D010936:Plant Extracts; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; C009469:thionine