title: The transgenic cloned pig population with integrated and controllable GH expression that has higher feed efficiency and meat production.
abstract: Sustained expression of the GH gene has been shown to have detrimental effects on the health of animals. In the current study, transgenic founder pigs, with controllable pig growth hormone (pGH) expression, were cloned via the handmade cloning method (HMC), and pGH expression levels were examined at the cellular and organismal levels. The serum pGH levels in 3 founder male pigs were found to be significantly higher after induction with intramuscular injection of doxycycline (DOX) compared to baseline. A daily dose of DOX was administered via feed to these animals for a period of 65 to 155 days. The growth rate, feed efficiency and pGH serum concentration increased in the DOX-induced transgenic group compared with the other groups. 8 numbers of animals were euthanized and the dressing percentage, loin muscle and lean meat percentage were significantly higher in the DOX-induced F1 transgenic group compared with the other groups. In this study a large population of transgenic pigs, with integrated controllable expression of a transgene, was obtained. The transgenic pigs were healthy and normal in terms of reproductive capability. At the same time, feed efficiency was improved, production processes were accelerated and meat yield was increased.
authors.affiliation: 1] Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, P. R. China [2] College of Veterinary Medicine, Yangzhou University, Yangzhou 25009, Jiangsu, P. R. China.
authors.forename: Huiming
authors.initials: H
authors.lastname: Ju
authors.affiliation: 1] Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, P. R. China [2] Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, P. R. China.
authors.forename: Jiaqing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, P. R. China.
authors.forename: Lijing
authors.initials: L
authors.lastname: Bai
authors.affiliation: Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, P. R. China.
authors.forename: Yulian
authors.initials: Y
authors.lastname: Mu
authors.affiliation: BGI-Shenzhen, Bei Shan Road, Yantian, Shenzhen, 518083, P. R. China.
authors.forename: Yutao
authors.initials: Y
authors.lastname: Du
authors.affiliation: BGI-Shenzhen, Bei Shan Road, Yantian, Shenzhen, 518083, P. R. China.
authors.forename: Wenxian
authors.initials: W
authors.lastname: Yang
authors.affiliation: BGI-Shenzhen, Bei Shan Road, Yantian, Shenzhen, 518083, P. R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Yangzhou University, Yangzhou 25009, Jiangsu, P. R. China.
authors.forename: Anzhi
authors.initials: A
authors.lastname: Sheng
authors.affiliation: Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, P. R. China.
authors.forename: Kui
authors.initials: K
authors.lastname: Li
mesh_terms: D000821:Animal Feed; D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D001724:Birth Weight / Q000187:drug effects; D002460:Cell Line; D002999:Clone Cells; D019976:Cloning, Organism; D004318:Doxycycline / Q000494:pharmacology; D004622:Embryo, Mammalian / Q000166:cytology; D005260:Female; D005333:Fetus / Q000166:cytology / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D018628:Gene Dosage; D013006:Growth Hormone / Q000097:blood / Q000235:genetics*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D008297:Male; D008460:Meat; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000235:genetics; D012098:Reproduction / Q000187:drug effects; D019849:Sex Determination Processes; D034421:Sus scrofa / Q000235:genetics*; D019076:Transgenes
chemical_list: D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone; D004318:Doxycycline