title: Development and evaluation of a real-time EBOV-L-RT-qPCR for detection of Zaire ebolavirus.
abstract: An RT-qPCR targeting EBOV-L including the preceding RNA extraction protocol were set up and evaluated.
authors.affiliation: Helsinki University Hospital, Department of Virology and Immunology, Finland; University of Helsinki, Department of Virology, Finland. Electronic address: anne.jaaskelainen@helsinki.fi.
authors.forename: Anne J
authors.initials: AJ
authors.lastname: Jääskeläinen
authors.affiliation: University of Helsinki, Department of Virology, Finland.
authors.forename: Kirsi
authors.initials: K
authors.lastname: Moilanen
authors.affiliation: University of Helsinki, Department of Virology, Finland.
authors.forename: Kirsi
authors.initials: K
authors.lastname: Aaltonen
authors.affiliation: University of Helsinki, Department of Virology, Finland.
authors.forename: Niina
authors.initials: N
authors.lastname: Putkuri
authors.affiliation: University of Helsinki, Department of Virology, Finland.
authors.forename: Tarja
authors.initials: T
authors.lastname: Sironen
authors.affiliation: Helsinki University Hospital, Department of Virology and Immunology, Finland; University of Helsinki, Department of Virology, Finland.
authors.forename: Hannimari
authors.initials: H
authors.lastname: Kallio-Kokko
authors.affiliation: Helsinki University Hospital, Department of Virology and Immunology, Finland; University of Helsinki, Department of Virology, Finland; Faculty of Veterinary Medicine, Department of Veterinary Biosciences, University of Helsinki, Helsinki, Finland.
authors.forename: Olli
authors.initials: O
authors.lastname: Vapalahti
mesh_terms: D029043:Ebolavirus / Q000302:isolation & purification*; D019142:Hemorrhagic Fever, Ebola / Q000175:diagnosis*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
chemical_list: D012367:RNA, Viral
keywords: Diagnostics; EBOV; PCR