title: Highly sensitive and robust peroxidase-like activity of porous nanorods of ceria and their application for breast cancer detection.
abstract: Porous nanorods of ceria (PN-Ceria), a novel ceria nanostructure with a large surface area and a high surface Ce(3+) fraction, exhibited strong intrinsic peroxidase activity toward a classical peroxidase substrate in the presence of H2O2. Peroxidase-like activity of ceria originated from surface Ce(3+) species as the catalytic center, thereby explaining the high performance of PN-Ceria as an artificial enzyme mimicking peroxidase. Compared with the natural enzyme horseradish peroxidase (HRP), PN-Ceria showed several advantages such as low cost, easy storage, high sensitivity, and, prominently, chemical and catalytic stability under harsh conditions. Importantly, the enzymatic activity of PN-Ceria remained nearly constant and stable over a wide range of temperature and pH values, ensuring the accuracy and reliability of measurements of its peroxidase-like activity. A PN-Ceria based novel diagnostic system was developed for breast cancer detection with a higher sensitivity than the standard HRP detection system. Our work has laid a solid foundation for the development of PN-Ceria as a novel diagnostic tool for clinical use.
authors.affiliation: Center for Applied Chemical Research, Frontier Institute of Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Tian
authors.affiliation: Center for Applied Chemical Research, Frontier Institute of Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Center for Applied Chemical Research, Frontier Institute of Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Zhiyun
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Center for Applied Chemical Research, Frontier Institute of Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Gao
authors.affiliation: Center for Applied Chemical Research, Frontier Institute of Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Center for Applied Chemical Research, Frontier Institute of Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China; State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China. Electronic address: yongquan@mail.xjtu.edu.cn.
authors.forename: Yongquan
authors.initials: Y
authors.lastname: Qu
mesh_terms: D001943:Breast Neoplasms / Q000175:diagnosis*; D002563:Cerium / Q000737:chemistry*; D005260:Female; D006801:Humans; D007700:Kinetics; D046529:Microscopy, Electron, Transmission; D043942:Nanotubes; D010544:Peroxidases / Q000378:metabolism*; D016062:Porosity
chemical_list: D002563:Cerium; D010544:Peroxidases
keywords: Biomimetic; Breast cancer detection; ELISA; Peroxidase mimetics; Porous nanorods of ceria