title: Biodegradable Microcarriers of Poly(Lactide-co-Glycolide) and Nano-Hydroxyapatite Decorated with IGF-1 via Polydopamine Coating for Enhancing Cell Proliferation and Osteogenic Differentiation.
abstract: In this study, insulin-like growth factor 1 (IGF-1) was successfully immobilized on the poly(lactide-co-glycolide)/hydroxyapatite (PLGA/HA) and pure PLGA microcarriers via polydopamine (pDA). The results demonstrated that the pDA layer facilitated simple and highly efficient immobilization of peptides on the microcarriers within 20 min. Mouse adipose-derived stem cells (ADSCs) attachment and proliferation on IGF-1-immobilized microcarriers were much higher than non-immobilized ones. More importantly, the IGF-1-immobilized PLGA/HA microcarriers significantly increased alkaline phosphatase (ALP) activity and expression of osteogenesis-related genes of ADSCs. Therefore, it is considered that the IGF-1-decorated PLGA/HA microcarriers will be of great value in the bone tissue engineering.
authors.affiliation: School of Public Health, Jilin University, Changchun, 130021, P. R. China.|Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China.
authors.forename: Tianlin
authors.initials: T
authors.lastname: Gao
authors.affiliation: Department of Foot and Ankle Surgery, The Second Hospital of Shandong University, Jinan, 250000, P. R. China.
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China.
authors.forename: Zongliang
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Public Health, Jilin University, Changchun, 130021, P. R. China. liuya@jlu.edu.cn.
authors.forename: Ya
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Nano Medical Engineering Laboratory, RIKEN, 2-1-Hirosawa, Wako, Saitama, 351-0198, Japan.|Emergent Bioengineering Materials Research Team, RIKEN Center for Emergent Materials Science, 2-1-Hirosawa, Wako, Saitama, 351-0198, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ito
authors.affiliation: Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China. zhangpb@ciac.ac.cn.
authors.forename: Peibiao
authors.initials: P
authors.lastname: Zhang
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000469:Alkaline Phosphatase / Q000096:biosynthesis; D000818:Animals; D063566:Biodegradable Plastics / Q000737:chemistry* / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology; D055767:Immobilized Proteins / Q000737:chemistry / Q000494:pharmacology; D007211:Indoles / Q000737:chemistry; D007334:Insulin-Like Growth Factor I / Q000737:chemistry* / Q000378:metabolism; D051379:Mice; D010012:Osteogenesis / Q000187:drug effects*; D011098:Polyglactin 910 / Q000737:chemistry / Q000494:pharmacology; D011108:Polymers / Q000737:chemistry; D013234:Stem Cells / Q000187:drug effects
chemical_list: D063566:Biodegradable Plastics; D055767:Immobilized Proteins; D007211:Indoles; D011108:Polymers; C568283:polydopamine; D011098:Polyglactin 910; D007334:Insulin-Like Growth Factor I; D017886:Durapatite; D000469:Alkaline Phosphatase
keywords: IGF-1; biodegradable PLGA microcarriers; bone tissue engineering; nano-hydroxyapatite; polydopamine