title: Vasorelaxant and Hypotensive Effects of Jaboticaba Fruit (Myrciaria cauliflora) Extract in Rats.
abstract: This study's aim was to determine the effect of hydroalcoholic extract of M. cauliflora (HEMC) on vascular tension and blood pressure in rats. In our in vitro studies using precontracted isolated aortas from rats, HEMC and acetylcholine (positive control) induced relaxation only in vessels with endothelium. Pretreatment with L-NAME (NO synthase inhibitor) or ODQ (soluble guanylyl cyclase (sGC) inhibitor) abolished the HEMC-induced relaxation. The treatment with MDL-12,330A (adenylyl cyclase (AC) inhibitor) or diclofenac (COX inhibitor) reduced HEMC-induced vasorelaxation. The blockade of muscarinic and β-adrenergic receptors (by atropine and propranolol, resp.) did not promote changes in HEMC-induced vasorelaxation. In our in vivo studies, catheters were inserted into the right femoral vein and artery of anesthetized rats for HEMC infusion and the measurement of blood pressure, heart rate, and aortic blood flow. The intravenous infusion of HEMC produced hypotension and increased aortic blood flow with no changes in heart rate. These findings showed that HEMC induces endothelium-dependent vascular relaxation and hypotension with no alteration in heart rate. The NO/sGC/cGMP pathway seems to be the main cellular route involved in the vascular responsiveness.
authors.affiliation: Faculty of Pharmacy, Federal University of Goias, Rua 240, Esquina com 5 Avenida, s/n, 74605-170 Goiânia, GO, Brazil.
authors.forename: Daniela Medeiros
authors.initials: DM
authors.lastname: Lobo de Andrade
authors.affiliation: Faculty of Pharmacy, Federal University of Goias, Rua 240, Esquina com 5 Avenida, s/n, 74605-170 Goiânia, GO, Brazil.
authors.forename: Carolina de Fátima
authors.initials: Cde F
authors.lastname: Reis
authors.affiliation: Faculty of Pharmacy, Federal University of Goias, Rua 240, Esquina com 5 Avenida, s/n, 74605-170 Goiânia, GO, Brazil.
authors.forename: Patrícia Ferreira da Silva
authors.initials: PF
authors.lastname: Castro
authors.affiliation: Faculty of Pharmacy, Federal University of Goias, Rua 240, Esquina com 5 Avenida, s/n, 74605-170 Goiânia, GO, Brazil.
authors.forename: Leonardo Luiz
authors.initials: LL
authors.lastname: Borges
authors.affiliation: Center for Neuroscience and Cardiovascular Research, Biological Sciences Institute, Federal University of Goias, Campus Samambaia, 74001-970 Goiânia, GO, Brazil.
authors.forename: Nathalia Oda
authors.initials: NO
authors.lastname: Amaral
authors.affiliation: Faculty of Pharmacy, Federal University of Goias, Rua 240, Esquina com 5 Avenida, s/n, 74605-170 Goiânia, GO, Brazil.
authors.forename: Ieda Maria Sapateiro
authors.initials: IM
authors.lastname: Torres
authors.affiliation: Faculty of Pharmacy, Federal University of Goias, Rua 240, Esquina com 5 Avenida, s/n, 74605-170 Goiânia, GO, Brazil.
authors.forename: Stefani Garcia
authors.initials: SG
authors.lastname: Rezende
authors.affiliation: Faculty of Pharmacy, Federal University of Goias, Rua 240, Esquina com 5 Avenida, s/n, 74605-170 Goiânia, GO, Brazil.
authors.forename: Eric de Souza
authors.initials: Ede S
authors.lastname: Gil
authors.affiliation: Faculty of Pharmacy, Federal University of Goias, Rua 240, Esquina com 5 Avenida, s/n, 74605-170 Goiânia, GO, Brazil.
authors.forename: Edemilson
authors.initials: E
authors.lastname: Cardoso da Conceição
authors.affiliation: Center for Neuroscience and Cardiovascular Research, Biological Sciences Institute, Federal University of Goias, Campus Samambaia, 74001-970 Goiânia, GO, Brazil.
authors.forename: Gustavo Rodrigues
authors.initials: GR
authors.lastname: Pedrino
authors.affiliation: Faculty of Pharmacy, Federal University of Goias, Rua 240, Esquina com 5 Avenida, s/n, 74605-170 Goiânia, GO, Brazil.
authors.forename: Matheus
authors.initials: M
authors.lastname: Lavorenti Rocha