title: Culture of human cell lines by a pathogen-inactivated human platelet lysate.
abstract: Alternatives to the use of fetal bovine serum (FBS) have been investigated to ensure xeno-free growth condition. In this study we evaluated the efficacy of human platelet lysate (PL) as a substitute of FBS for the in vitro culture of some human cell lines. PL was obtained by pools of pathogen inactivated human donor platelet (PLT) concentrates. Human leukemia cell lines (KG-1, K562, JURKAT, HL-60) and epithelial tumor cell lines (HeLa and MCF-7) were cultured with either FBS or PL. Changes in cell proliferation, viability, morphology, surface markers and cell cycle were evaluated for each cell line. Functional characteristics were analysed by drug sensitivity test and cytotoxicity assay. Our results demonstrated that PL can support growth and expansion of all cell lines, although the cells cultured in presence of PL experienced a less massive proliferation compared to those grown with FBS. We found a comparable percentage of viable specific marker-expressing cells in both conditions, confirming lineage fidelity in all cultures. Functionality assays showed that cells in both FBS- and PL-supported cultures maintained their normal responsiveness to adriamycin and NK cell-mediated lysis. Our findings indicate that PL is a feasible serum substitute for supporting growth and propagation of haematopoietic and epithelial cell lines with many advantages from a perspective of process standardization, ethicality and product safety.
authors.affiliation: InScientiaFides Foundation, San Marino, Republic of San Marino.
authors.forename: R
authors.initials: R
authors.lastname: Fazzina
authors.affiliation: Immunohematology and Transfusion Medicine, San Camillo-Forlanini Hospital, Rome, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Iudicone
authors.affiliation: Institute of Gynecology and Obstetrics, Sacred Heart Catholic University, Rome, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Mariotti
authors.affiliation: Immunohematology and Transfusion Medicine, San Camillo-Forlanini Hospital, Rome, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Fioravanti
authors.affiliation: Institute of Gynecology and Obstetrics, Sacred Heart Catholic University, Rome, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Procoli
authors.affiliation: Immunohematology and Transfusion Medicine, San Camillo-Forlanini Hospital, Rome, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Cicchetti
authors.affiliation: Institute of Gynecology and Obstetrics, Sacred Heart Catholic University, Rome, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Scambia
authors.affiliation: Institute of Gynecology and Obstetrics, Sacred Heart Catholic University, Rome, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Bonanno
authors.affiliation: Immunohematology and Transfusion Medicine, San Camillo-Forlanini Hospital, Rome, Italy. lpierelli@me.com.|Department of Experimental Medicine, Sapienza University, Rome, Italy. lpierelli@me.com.
authors.forename: L
authors.initials: L
authors.lastname: Pierelli
keywords: Fetal bovine serum; Human cell lines; Platelet lysate; Xeno-free conditions