title: Discrimination of Semen cassiae from two related species based on the multivariate analysis of high-performance liquid chromatography fingerprints.
abstract: A simple and efficient high-performance liquid chromatography fingerprint method was developed to discriminate Semen cassiae from two related species: Cassia obtusifolia L. (CO) and Cassia tora L. (CT), the seeds of which are abbreviated as COS and CTS, respectively. 22 major bioactive ingredients in 42 samples (20 COS and 22 CTS) collected from different provinces of China were identified. The statistical methods included similarity analysis and partial least-squares discriminant analysis. The pattern analysis method was specific and could be readily used for the comprehensive evaluation of Semen cassiae samples. Therefore, high-performance liquid chromatography fingerprint in combination with pattern analysis provided a simple and reliable method for discriminating between COS and CTS.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.
authors.forename: Liying
authors.initials: L
authors.lastname: Tang
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Wu
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.
authors.forename: Xidan
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.
authors.forename: Yilong
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.
authors.forename: Guohong
authors.initials: G
authors.lastname: Zhou
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Kou
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.
authors.forename: Zhuju
authors.initials: Z
authors.lastname: Wang
mesh_terms: D002366:Cassia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D016018:Least-Squares Analysis; D015999:Multivariate Analysis; D010936:Plant Extracts / Q000032:analysis*; D011786:Quality Control; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry*; D013045:Species Specificity
chemical_list: D004365:Drugs, Chinese Herbal; D010936:Plant Extracts
keywords: Cassia obtusifolia L; Cassia tora L; Fingerprint analysis; High-performance liquid chromatography; Multivariate analysis