title: Biofunction-assisted aptasensors based on ligand-dependent 3' processing of a suppressor tRNA in a wheat germ extract.
abstract: We have developed a novel type of biofunction-assisted aptasensor that harnesses ligand-dependent 3' processing of a premature amber suppressor tRNA and the subsequent amber suppression of a reporter gene in a wheat germ extract.
authors.affiliation: Proteo-Science Center, Ehime University, 3 Bunkyo-cho, Matsuyama, Ehime 790-8577, Japan. ogawa.atsushi.mf@ehime-u.ac.jp.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ogawa
authors.forename: Junichiro
authors.initials: J
authors.lastname: Tabuchi
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000235:genetics; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D017930:Genes, Reporter; D010936:Plant Extracts / Q000737:chemistry / Q000235:genetics*; D012343:RNA, Transfer / Q000737:chemistry* / Q000235:genetics; D013489:Suppression, Genetic; D013806:Theophylline / Q000032:analysis*; D014908:Triticum / Q000737:chemistry / Q000235:genetics*; D014665:Vasodilator Agents / Q000032:analysis*
chemical_list: D052157:Aptamers, Nucleotide; D010936:Plant Extracts; D014665:Vasodilator Agents; D012343:RNA, Transfer; D013806:Theophylline