title: Efficient construction of stable gene nanoparticles through polymerase chain reaction with flexible branched primers for gene delivery.
abstract: Flexible branched primers were designed to construct stable gene nanoparticles with multiple target gene copies through polymerase chain reaction, which can be used as an efficient transcription template in eukaryotic cells for gene delivery.
authors.affiliation: Department of Chemical Biology, State Key Laboratory of Elemento-Organic Chemistry, National Engineering Research Center of Pesticide (Tianjin), Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Tianjin 300071, China. zhenxi@nankai.edu.cn.
authors.forename: Jianbing
authors.initials: J
authors.lastname: Liu
authors.forename: Runyu
authors.initials: R
authors.lastname: Wang
authors.forename: Dejun
authors.initials: D
authors.lastname: Ma
authors.forename: Di
authors.initials: D
authors.lastname: Ouyang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Xi
mesh_terms: D003587:Cytomegalovirus / Q000235:genetics; D004247:DNA / Q000737:chemistry*; D017931:DNA Primers; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000235:genetics; D057809:HEK293 Cells; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D004247:DNA