title: Serum Bile Acids Are Associated with Pathological Progression of Hepatitis B-Induced Cirrhosis.
abstract: Recent metabonomic studies have identified an important role of bile acids in patients with liver cirrhosis. Serum bile acids, such as glycocholate (GCA), glycochenodeoxycholate (GCDCA), taurocholate (TCA), and taurochenodeoxycholate (TCDCA), increased significantly in liver cirrhosis patients. Our recently published urinary metabonomic study showed that glycocholate 3-glucuronide, taurohyocholate, TCA, glycolithocholate 3-sulfate, and glycoursodeoxycholate (GUDCA) were markedly increased in hepatitis B-induced cirrhotic patients (n = 63) compared with healthy controls (n = 31). The urinary levels of GUDCA were able to differentiate among three stages of cirrhotic patients with Child-Pugh (CP) score A, B, and C. In this study, we recruited two new cohorts of patients with hepatitis-B-induced cirrhosis and healthy control subjects and quantitatively profiled their serum bile acids using ultra-performance liquid chromatography triple quadrupole mass spectrometry. Serum bile acid profile and corresponding differential bile acids were characterized, in addition to the blood routine, liver, and renal function tests. The alterations of bile acids contributing to the intergroup variation between healthy controls and cirrhotic patients and among pathological stages of CP grade A, B and C were also investigated. Five bile acids, GCA, GCDCA, TCA, TCDCA, and GUDCA, were significantly altered among different stages of liver cirrhosis (n = 85), which was validated with an independent cohort of cirrhotic patients (n = 53). Our results show that dynamic alteration of serum bile acids is indicative of an exacerbated liver function, highlighting their potential as biomarkers for staging the liver cirrhosis and monitoring its progression.
authors.affiliation: E-institute of Shanghai Municipal Education Committee, Shanghai University of Traditional Chinese Medicine , 1200 Cailun Road, Shanghai 201203, China.|Key Laboratory of Liver and Kidney Diseases (Ministry of Education), Institute of Liver Diseases, Shuguang Hospital , 528 Zhangheng Road, Shanghai 201204, China.
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Wang
authors.affiliation: University of Hawaii Cancer Center , 701 Ilalo Street, Honolulu, Hawaii 96813, United States.|Shanghai Key Laboratory of Diabetes Mellitus and Center for Translational Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital , 600 Yishan Road, Shanghai 200233, China.
authors.forename: Guoxiang
authors.initials: G
authors.lastname: Xie
authors.affiliation: Shanghai Key Laboratory of Diabetes Mellitus and Center for Translational Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital , 600 Yishan Road, Shanghai 200233, China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Zhao
authors.affiliation: Shanghai Key Laboratory of Diabetes Mellitus and Center for Translational Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital , 600 Yishan Road, Shanghai 200233, China.
authors.forename: Xiaojiao
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Shanghai Key Laboratory of Diabetes Mellitus and Center for Translational Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital , 600 Yishan Road, Shanghai 200233, China.
authors.forename: Fengjie
authors.initials: F
authors.lastname: Huang
authors.affiliation: Key Laboratory of Liver and Kidney Diseases (Ministry of Education), Institute of Liver Diseases, Shuguang Hospital , 528 Zhangheng Road, Shanghai 201204, China.
authors.forename: Yixing
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Guangxi University of Traditional Chinese Medicine , 179 East Mingxiu Road, Nanning, Guangxi 530001, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Yao
authors.affiliation: University of Hawaii Cancer Center , 701 Ilalo Street, Honolulu, Hawaii 96813, United States.|Shanghai Key Laboratory of Diabetes Mellitus and Center for Translational Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital , 600 Yishan Road, Shanghai 200233, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Jia
authors.affiliation: E-institute of Shanghai Municipal Education Committee, Shanghai University of Traditional Chinese Medicine , 1200 Cailun Road, Shanghai 201203, China.|Key Laboratory of Liver and Kidney Diseases (Ministry of Education), Institute of Liver Diseases, Shuguang Hospital , 528 Zhangheng Road, Shanghai 201204, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
mesh_terms: D000328:Adult; D001647:Bile Acids and Salts / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D002853:Chromatography, Liquid; D016002:Discriminant Analysis; D018450:Disease Progression; D005260:Female; D006509:Hepatitis B / Q000097:blood* / Q000175:diagnosis / Q000473:pathology* / Q000821:virology; D006515:Hepatitis B virus / Q000502:physiology; D006801:Humans; D007677:Kidney Function Tests; D008099:Liver / Q000378:metabolism / Q000473:pathology / Q000821:virology; D008103:Liver Cirrhosis / Q000097:blood* / Q000175:diagnosis / Q000473:pathology* / Q000821:virology; D008111:Liver Function Tests; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged
chemical_list: D001647:Bile Acids and Salts; D015415:Biomarkers
keywords: Child-Pugh classification; bile acid; cirrhosis; hepatitis B; serum