title: Protective effect of theophylline on renal functions in experimental pneumoperitoneum model.
abstract: Our objective in this experimental study is to research the effect of the intra-abdominal pressure which rises following pneumoperitoneum and whether Theophylline has a possible protective activity on this situation. In our study, 24 Wistar Albino rats were used. Rats were divided into two groups. The first group was set for only pneumoperitoneum model. The second group was given 15 mg/kg of Theophylline intraperitoneally before setting pneumoperitoneum model. Then urea, creatinine, cystatin-C, tissue and serum total antioxidant capacity, total oxidant capacity and oxidative stress index in two groups were measured and compared with each other. Apoptosis and histopathological conditions in the renal tissues were examined. The differences between the groups were analyzed with the Mann-Whitney U test. Results were considered significant at p < 0.05. No statistically significant difference was determined between tissue and serum averages in two groups in terms of TAS, TOS and OSI values (p > 0.05). The mean value of urea were similar in pneumoperitoneum and pneumoperitoneum + theophylline groups (p = 0.12). The mean cystatin-C value was 2.2 ± 0.3 µg/mL in pneumoperitoneum, 1.74 ± 0.33 µg/mL in pneumoperitoneum + theophylline (p = 0.002). According to our study, lower cystatin-C levels in the group, where Theophylline was given, are suggestive of lower renal injury in this group. However, this opinion is interrogated as there is no difference in terms of tissue and serum TAS, TOS, OSI and urea values between the groups.
authors.affiliation: a Department of Urology Clinic , Suleyman Demirel University, Medical Faculty , Isparta , Turkey .
authors.forename: Sefa Alperen
authors.initials: SA
authors.lastname: Ozturk
authors.affiliation: b Department of Urology Clinic , Yüksek Ihtisas Training and Research Hospital , Ankara , Turkey .
authors.forename: Cavit
authors.initials: C
authors.lastname: Ceylan
authors.affiliation: a Department of Urology Clinic , Suleyman Demirel University, Medical Faculty , Isparta , Turkey .
authors.forename: Tekin Ahmet
authors.initials: TA
authors.lastname: Serel
authors.affiliation: c Department of Urology Clinic , Ankara Training and Research Hospital , Ankara , Turkey .
authors.forename: Omer Gokhan
authors.initials: OG
authors.lastname: Doluoglu
authors.affiliation: a Department of Urology Clinic , Suleyman Demirel University, Medical Faculty , Isparta , Turkey .
authors.forename: Arap Sedat
authors.initials: AS
authors.lastname: Soyupek
authors.affiliation: a Department of Urology Clinic , Suleyman Demirel University, Medical Faculty , Isparta , Turkey .
authors.forename: Ahmet
authors.initials: A
authors.lastname: Guzel
authors.affiliation: a Department of Urology Clinic , Suleyman Demirel University, Medical Faculty , Isparta , Turkey .
authors.forename: Alper
authors.initials: A
authors.lastname: Özorak
authors.affiliation: d Department of Biochemistry , and.
authors.forename: Efkan
authors.initials: E
authors.lastname: Uz
authors.affiliation: d Department of Biochemistry , and.
authors.forename: Hasan Basri
authors.initials: HB
authors.lastname: Savas
authors.affiliation: e Department of Pathology , Medical Faculty, Suleyman Demirel University , Isparta , Turkey.
authors.forename: Sirin
authors.initials: S
authors.lastname: Baspinar
mesh_terms: D058186:Acute Kidney Injury / Q000517:prevention & control*; D000818:Animals; D015415:Biomarkers / Q000097:blood; D001707:Biopsy, Needle; D003401:Creatine / Q000097:blood; D055316:Cystatin C / Q000097:blood; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D007274:Injections, Intraperitoneal; D007677:Kidney Function Tests; D007813:Laparotomy; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D011028:Pneumoperitoneum, Artificial / Q000379:methods*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*; D012680:Sensitivity and Specificity; D013806:Theophylline / Q000494:pharmacology*; D014508:Urea / Q000097:blood
chemical_list: D015415:Biomarkers; D055316:Cystatin C; D014508:Urea; D013806:Theophylline; D003401:Creatine
keywords: Laparoscopy; pneumoperitoneum; rat; renal function; theophylline