title: Improvement Characteristics of Bio-active Materials Coated Fabric on Rat Muscular Mitochondria.
abstract: This study surveys the improvement characteristics in old-aged muscular mitochondria by bio-active materials coated fabric (BMCF). To observe the effects, the fabric (10 and 30%) was worn to old-aged rat then the oxygen consumption efficiency and copy numbers of mitochondria, and mRNA expression of apoptosis- and mitophagy-related genes were verified. By wearing the BMCF, the oxidative respiration significantly increased when using the 30% materials coated fabric. The mitochondrial DNA copy number significantly decreased and subsequently recovered in a dose-dependent manner. The respiratory control ratio to mitochondrial DNA copy number showed a dose-dependent increment. As times passed, Bax, caspase 9, PGC-1α and β-actin increased, and Bcl-2 decreased in a dose-dependent manner. However, the BMCF can be seen to have had no effect on Fas receptor. PINK1 expression did not change considerably and was inclined to decrease in control group, but the expression was down-regulated then subsequently increased with the use of the BMCF in a dose-dependent manner. Caspase 3 increased and subsequently decreased in a dose-dependent manner. These results suggest that the BMCF invigorates mitophagy and improves mitochondrial oxidative respiration in skeletal muscle, and in early stage of apoptosis induced by the BMCF is not related to extrinsic death-receptor mediated but mitochondria-mediated signaling pathway.
authors.affiliation: Department of Physiology, College of Medicine, Chung-Ang University, Seoul 156-756, Korea.
authors.forename: Donghee
authors.initials: D
authors.lastname: Lee
authors.affiliation: Department of Physiology, College of Medicine, Chung-Ang University, Seoul 156-756, Korea.
authors.forename: Young-Won
authors.initials: YW
authors.lastname: Kim
authors.affiliation: Department of Family Medicine, College of Medicine, Chung-Ang University, Seoul 156-756, Korea.
authors.forename: Jung-Ha
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Physiology, College of Medicine, Chung-Ang University, Seoul 156-756, Korea.
authors.forename: Misuk
authors.initials: M
authors.lastname: Yang
authors.affiliation: Department of Physiology, College of Medicine, Chung-Ang University, Seoul 156-756, Korea.
authors.forename: Hyemi
authors.initials: H
authors.lastname: Bae
authors.affiliation: Department of Physiology, College of Medicine, Chung-Ang University, Seoul 156-756, Korea.
authors.forename: Inja
authors.initials: I
authors.lastname: Lim
authors.affiliation: Department of Physiology, College of Medicine, Chung-Ang University, Seoul 156-756, Korea.
authors.forename: Hyoweon
authors.initials: H
authors.lastname: Bang
authors.affiliation: Research and Development Center, VENTEX Co. Ltd., Seoul 138-220, Korea.
authors.forename: Kyung-Chan
authors.initials: KC
authors.lastname: Go
authors.affiliation: Research and Development Center, VENTEX Co. Ltd., Seoul 138-220, Korea.
authors.forename: Gwang-Wung
authors.initials: GW
authors.lastname: Yang
authors.affiliation: Research and Development Center, VENTEX Co. Ltd., Seoul 138-220, Korea.
authors.forename: Yong-Hwan
authors.initials: YH
authors.lastname: Rho
authors.affiliation: Research and Development Center, VENTEX Co. Ltd., Seoul 138-220, Korea.
authors.forename: Hyo-Suk
authors.initials: HS
authors.lastname: Park
authors.affiliation: Research and Development Center, VENTEX Co. Ltd., Seoul 138-220, Korea.
authors.forename: Eun-Ho
authors.initials: EH
authors.lastname: Park
authors.affiliation: Department of Physiology, College of Medicine, Chung-Ang University, Seoul 156-756, Korea.
authors.forename: Jae-Hong
authors.initials: JH
authors.lastname: Ko
keywords: Apoptosis; Bio-active materials coated fabric; Mitochondria; Mitophagy; Oxidative respiration