title: Backbone chemical shift assignments for the sensor domain of the Burkholderia pseudomallei histidine kinase RisS: "missing" resonances at the dimer interface.
abstract: Using a deuterated sample, all the observable backbone (1)H(N), (15)N, (13)C(a), and (13)C' chemical shifts for the dimeric, periplasmic sensor domain of the Burkholderia pseudomallei histidine kinase RisS were assigned. Approximately one-fifth of the amide resonances are "missing" in the (1)H-(15)N HSQC spectrum and map primarily onto α-helices at the dimer interface observed in a crystal structure suggesting this region either undergoes intermediate timescale motion (μs-ms) and/or is heterogeneous.
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease, Seattle, USA. garry.buchko@pnnl.gov.|Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, 99352, USA. garry.buchko@pnnl.gov.
authors.forename: Garry W
authors.initials: GW
authors.lastname: Buchko
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease, Seattle, USA.|Beryllium, Bainbridge Island, WA, 98110, USA.
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Edwards
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease, Seattle, USA.|Department of Medicine, University of Washington, Seattle, WA, 98195-7185, USA.
authors.forename: Stephen N
authors.initials: SN
authors.lastname: Hewitt
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease, Seattle, USA.|Center for Infectous Disease Research, formerly Seattle Biomedical Research Institute, Seattle, WA, 98109-5219, USA.
authors.forename: Isabelle Q H
authors.initials: IQ
authors.lastname: Phan
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease, Seattle, USA.|Department of Medicine, University of Washington, Seattle, WA, 98195-7185, USA.
authors.forename: Wesley C
authors.initials: WC
authors.lastname: Van Voorhis
authors.affiliation: Department of Medicine, University of Washington, Seattle, WA, 98195-7185, USA.|Department of Microbiology and Department of Genome Sciences, University of Washington, Seattle, WA, 98195, USA.
authors.forename: Samuel I
authors.initials: SI
authors.lastname: Miller
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease, Seattle, USA.|Center for Infectous Disease Research, formerly Seattle Biomedical Research Institute, Seattle, WA, 98109-5219, USA.|Department of Biomedical Informatics and Medical Education and Department of Global Health, University of Washington, Seattle, WA, 98195, USA.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Myler
mesh_terms: D000595:Amino Acid Sequence; D016957:Burkholderia pseudomallei / Q000378:metabolism*; D000071677:Histidine Kinase; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D011494:Protein Kinases / Q000737:chemistry*; D055503:Protein Multimerization; D017434:Protein Structure, Tertiary; D066244:Proton Magnetic Resonance Spectroscopy
chemical_list: D011494:Protein Kinases; D000071677:Histidine Kinase
keywords: Chemical shift perturbations; Infectious diseases; Meliodosis; Signal transduction; Two-component system