title: 28-mer Fragment Derived from Enterocin CRL35 Displays an Unexpected Bactericidal Effect on Listeria Cells.
abstract: Two shorter peptides derived from enterocin CRL35, a 43-mer bacteriocin, were synthesized i.e. the N-terminal fragment spanning from residues 1 to 15, and a 28-mer fragment that represents the C-terminal of enterocin CRL35, the residues 16 to 43. The separate peptides showed no activity when combined. On one hand, the 28-mer peptide displayed an unpredicted antimicrobial activity. On the other, 15- mer peptide had no consistent anti-Listeria effect. The dissociation constants calculated from experimental data indicated that all peptides could bind at similar extent to the sensitive cells. However, transmembrane electrical potential was not dissipated to the same level by the different peptides; whereas the full-length and the C-terminal 28-mer fragment induced almost full dissipation, 15-mer fragment produced only a slow and incomplete effect. Furthermore, a different interaction of each peptide with membranes was demonstrated based on studies carried out with liposomes, which led us to conclude that activity was related to structure rather than to net positive charges. These results open up the possibility of designing new peptides based on the 28-mer fragment with enhanced activity, which would represent a promising approach for combating Listeria and other pathogens.
authors.forename: Emilse
authors.initials: E
authors.lastname: Masias
authors.forename: Paulo R S
authors.initials: PR
authors.lastname: Sanches
authors.forename: Fernando G
authors.initials: FG
authors.lastname: Dupuy
authors.forename: Leonardo
authors.initials: L
authors.lastname: Acuna
authors.forename: Augusto
authors.initials: A
authors.lastname: Bellomio
authors.forename: Eduardo
authors.initials: E
authors.lastname: Cilli
authors.forename: Lucila
authors.initials: L
authors.lastname: Saavedra
authors.affiliation: INSIBIO (CONICET/UNT) and Facultad de Bioquímica, Quimica y Farmacia, Universidad Nacional de Tucuman, SM de Tucuman, Argentina. cminahk@fbqf.unt.edu.ar.
authors.forename: Carlos
authors.initials: C
authors.lastname: Minahk
mesh_terms: D000595:Amino Acid Sequence; D001430:Bacteriocins / Q000138:chemical synthesis / Q000737:chemistry* / Q000235:genetics / Q000494:pharmacology*; D007777:Lactobacillaceae / Q000235:genetics; D008081:Liposomes / Q000737:chemistry; D008087:Listeria / Q000187:drug effects*; D008564:Membrane Potentials / Q000187:drug effects; D050296:Microbial Viability / Q000187:drug effects; D008969:Molecular Sequence Data
chemical_list: D001430:Bacteriocins; D008081:Liposomes; C121783:enterocin CRL35