title: Development of Bioluminescent Cronobacter sakazakii ATCC 29544 in a Mouse Model.
abstract: Cronobacter sakazakii is an emerging pathogen that causes severe and life-threatening conditions including meningitis, bacteremia, and necrotizing enterocolitis. An animal model study for extrapolation of C. sakazakii infection can provide a better understanding of pathogenesis. However, methods for real-time monitoring of the course of C. sakazakii infection in living animals have been lacking. We developed a bioluminescent C. sakazakii strain (ATCC 29544) that can be used for real-time monitoring of C. sakazakii infection in BALB/c mice. C. sakazakii ATCC 29544 mainly colonized brain, liver, spleen, kidney, and gastrointestinal tract, as indicated by bioluminescence imaging. This work provides a novel approach for studying the progression of C. sakazakii infection and evaluating therapeutics in a living mouse model.
authors.affiliation: Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, People's Republic of China.
authors.forename: Xiwen
authors.initials: X
authors.lastname: Wang
authors.affiliation: Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, People's Republic of China.
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Li
authors.affiliation: Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, People's Republic of China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Dong
authors.affiliation: Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, People's Republic of China.
authors.forename: Hang
authors.initials: H
authors.lastname: Chi
authors.affiliation: National Engineering Laboratory for Druggable Gene and Protein Screening, Northeast Normal University, Changchun 130117, People's Republic of China.
authors.forename: Guannan
authors.initials: G
authors.lastname: Wang
authors.affiliation: Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, People's Republic of China.
authors.forename: Jiakuan
authors.initials: J
authors.lastname: Li
authors.affiliation: Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, People's Republic of China.
authors.forename: Rui
authors.initials: R
authors.lastname: Sun
authors.affiliation: Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, People's Republic of China.
authors.forename: Man
authors.initials: M
authors.lastname: Chen
authors.affiliation: Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, People's Republic of China.
authors.forename: Xinying
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, People's Republic of China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, People's Republic of China.
authors.forename: Han
authors.initials: H
authors.lastname: Qu
authors.affiliation: Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, People's Republic of China;, Email: zipxia@126.com.
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Xia
authors.affiliation: Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, People's Republic of China. lqxue@hotmail.com.
authors.forename: Qianxue
authors.initials: Q
authors.lastname: Li
mesh_terms: D000818:Animals; D044083:Cronobacter sakazakii / Q000302:isolation & purification*; D004195:Disease Models, Animal; D004756:Enterobacteriaceae Infections / Q000175:diagnosis*; D008163:Luminescent Measurements / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C