title: Paeoniflorin, a Monoterpene Glycoside, Protects the Brain from Cerebral Ischemic Injury via Inhibition of Apoptosis.
abstract: Paeoniflorin (PF) is a principal bioactive component, which exhibits many pharmacological effects, including protection against ischemic injury. This paper aimed to investigate the protective effect of PF both in vivo and in vitro. Middle cerebral artery occlusion (MCAO) was performed on male Sprague-Dawley (SD) rat for 2 h, and different doses of PF or vehicle were administered 2 h after reperfusion. Rats were sacrificed after 7 days treatment of PF/vehicle. PF treatment for 7 days ameliorated MCAO-induced neurological deficit and decreased the infarct area. Further study demonstrated that PF inhibited the over-activation of astrocytes and apoptosis of neurons, and PF promoted up-regulation of neuronal specific marker neuron-specific nuclear (NeuN) and microtubule-associated protein 2 (MAP-2) in brain. Moreover, NMDA-induced neuron apoptosis was employed. The in vitro study revealed that PF treatment protected against NMDA-induced cell apoptosis and neuronal loss via up-regulation of neuronal specific marker NeuN, MAP-2 and Bcl-2 and the down-regulation Bax. Taken together, the present study demonstrates that PF produces its protective effect by inhibiting the over-activation of astrocytes, apoptosis of neurons and up-regulation of neuronal specific marker NeuN, MAP-2, and B-cell lymphoma-2 (Bcl-2), and down-regulation Bax. Our study reveals that PF may be a potential neuroprotective agent for stroke and can provide basic data for clinical use.
authors.affiliation: College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P. R. China.
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Zhang
authors.forename: Huang
authors.initials: H
authors.lastname: Li
authors.forename: Mingqing
authors.initials: M
authors.lastname: Huang
authors.forename: Mei
authors.initials: M
authors.lastname: Huang
authors.forename: Kedan
authors.initials: K
authors.lastname: Chu
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Shengnan
authors.initials: S
authors.lastname: Zhang
authors.forename: Jinhua
authors.initials: J
authors.lastname: Que
authors.forename: Lidian
authors.initials: L
authors.lastname: Chen
mesh_terms: D000818:Animals; D034961:Antigens, Nuclear / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects*; D001253:Astrocytes / Q000473:pathology*; D001921:Brain / Q000378:metabolism; D002545:Brain Ischemia / Q000235:genetics / Q000473:pathology / Q000517:prevention & control*; D005960:Glucosides / Q000494:pharmacology* / Q000627:therapeutic use*; D006027:Glycosides / Q000494:pharmacology* / Q000627:therapeutic use*; D008297:Male; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D039821:Monoterpenes / Q000494:pharmacology* / Q000627:therapeutic use*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000473:pathology*; D018696:Neuroprotective Agents; D008517:Phytotherapy; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D034961:Antigens, Nuclear; D005960:Glucosides; D006027:Glycosides; D008869:Microtubule-Associated Proteins; D039821:Monoterpenes; D009419:Nerve Tissue Proteins; D018696:Neuroprotective Agents; C546339:Rbfox3 protein, rat; C015423:peoniflorin
keywords: GFAP; MAP-2; Middle Cerebral Artery Occlusion; NeuN; Paeoniflorin