title: The influence of Helicobacter pylori eradication on the expression and methylation status of the FHIT gene in non-cancerous gastric mucosa of dyspeptic patients.
abstract: OBJECTIVE
  To investigate the effect of Helicobacter pylori (H. pylori) eradication on the expression level of the FHIT gene and its methylation status in the gastric mucosa of dyspeptic patients with or without a family history of gastric cancer (FHGC).
  
  
  METHODS
  In all, 31 patients with H. pylori infection including 13 with FHGC were enrolled in the study. The effectiveness of H. pylori eradication were confirmed by UBT, RUT and multiplex PCR (the presence of selected H. pylori strains) for biopsy samples from the antrum and corpus. Histopathological assessment was also performed. The expression of FHIT mRNA was determined by quantitative reverse transcription-polymerase chain reaction and the methylation status of the FHIT promoter was assessed by methylation-specific polymerase chain reaction.
  
  
  RESULTS
  After H. pylori eradication, the improvement of inflammation from superficial gastritis to normal mucosa (G → N) was observed in 39% of the patients without FHGC and in 54% of those with FHGC. FHIT mRNA expression was increased in patients without FHGC after H. pylori eradication (P < 0.05), while there was no statistically significant change in gene methylation status after H. pylori eradication (P > 0.05). For the samples from those with FHGC, the FHIT mRNA expression was not significantly changed and the methylation status fluctuated evenly.
  
  
  CONCLUSIONS
  H. pylori eradication results in the improvement of gastric mucosal inflammation and histopathological non-atrophic changes. The FHIT gene expression is increased in patients without FHGC, which may contribute to the prevention of GC development.
authors.affiliation: Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Lodz, Poland.
authors.forename: Lukasz
authors.initials: L
authors.lastname: Peczek
authors.affiliation: Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Lodz, Poland.
authors.forename: Karolina
authors.initials: K
authors.lastname: Zuk
authors.affiliation: Department of Gastroenterology, Medical University of Lodz, Lodz, Poland.
authors.forename: Krystyna
authors.initials: K
authors.lastname: Stec-Michalska
authors.affiliation: Department of Gastroenterology, Medical University of Lodz, Lodz, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Medrek
authors.affiliation: Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Lodz, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Nawrot
mesh_terms: D017766:Acid Anhydride Hydrolases / Q000378:metabolism*; D000328:Adult; D004415:Dyspepsia / Q000235:genetics*; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism*; D005756:Gastritis; D015870:Gene Expression; D016481:Helicobacter Infections / Q000235:genetics* / Q000628:therapy; D016480:Helicobacter pylori; D006801:Humans; D008297:Male; D008745:Methylation; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000099170:Bis(5'-adenosyl)triphosphatase
chemical_list: D017766:Acid Anhydride Hydrolases; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D000099170:Bis(5'-adenosyl)triphosphatase
keywords: Helicobacter pylori; dyspepsia; eradication; fragile histidine triad protein; methylation; stomach neoplasms