title: Epigallocatechin-3-gallate-induced free-radical production upon adipogenic differentiation in bovine bone-marrow mesenchymal stem cells.
abstract: Epigallocatechin-3-gallate (EGCG), a major component of catechin in green tea, has known effects on cancer, diabetes and obesity. We recently reported that the expression levels of various genes and proteins involved in adipogenesis decreases following EGCG treatment. We also assessed apoptosis in EGCG-exposed cells. Here, we explore the variability in free-radical production in bovine bone-marrow mesenchymal stem cells (BMSCs) treated with EGCG. Upon adipogenic differentiation, BMSCs were exposed to various EGCG concentrations (0, 0.1, 1, 5, or 10 μM) for 2, 4, or 6 days. We found that EGCG reduced cell viability and arrested the cell cycle at the gap 2/mitosis phase and that EGCG potentially enhanced the production of free radicals, including reactive oxygen species and reactive nitrogen species, in a concentration- and time-dependent manner. Immunostaining revealed that the expression of genes encoding CCAAT/enhancer-binding protein alpha and stearoyl-CoA desaturase were diminished by EGCG treatment. These findings suggest that EGCG alters free-radical production activity during adipogenic differentiation in BMSCs.
authors.affiliation: Animal Products Utilization Division, National Institute of Animal Science, Rural Development Administration, 1500, Kongjwipatjwi-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do, 565-851, Republic of Korea.|Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration, 1500, Kongjwipatjwi-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do, 565-851, Republic of Korea.
authors.forename: Jin Young
authors.initials: JY
authors.lastname: Jeong
authors.affiliation: Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration, 1500, Kongjwipatjwi-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do, 565-851, Republic of Korea.
authors.forename: Mi Na
authors.initials: MN
authors.lastname: Park
authors.affiliation: Department of Animal Resources Development Swine Science Division, National Institute of Animal Science, Rural Development Adminstration, 114,Sinbang 1-St, Seonghwan-eup, Seobuk-gu, Cheonan-city, Chungcheongnam-do, 331-801, Republic of Korea.
authors.forename: Eun Seok
authors.initials: ES
authors.lastname: Cho
authors.affiliation: College of Phamacy, Dankook University, 119 Dandae-ro, Cheonan, 330-714, Republic of Korea.
authors.forename: Hyun-Jun
authors.initials: HJ
authors.lastname: Jang
authors.affiliation: Department of Food Science and Technology, Sejong University, 98 Gun-ja-dong, Seoul, 143-747, Republic of Korea.
authors.forename: Sungkwon
authors.initials: S
authors.lastname: Park
authors.affiliation: Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration, 1500, Kongjwipatjwi-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do, 565-851, Republic of Korea. hyunj68@korea.kr.
authors.forename: Hyun-Jeong
authors.initials: HJ
authors.lastname: Lee
mesh_terms: D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D001853:Bone Marrow / Q000378:metabolism*; D002392:Catechin / Q000031:analogs & derivatives* / Q000378:metabolism; D002417:Cattle; D002454:Cell Differentiation; D005609:Free Radicals / Q000378:metabolism*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D017382:Reactive Oxygen Species; D013662:Tea / Q000378:metabolism*
chemical_list: D005609:Free Radicals; D017382:Reactive Oxygen Species; D013662:Tea; D002392:Catechin; C045651:epigallocatechin gallate
keywords: Adipogenic differentiation; Bone-marrow mesenchymal stem cells; Bovine; Epigallocatechin-3-gallate; Free radicals