title: Home-based urinary HPV DNA testing in women who do not attend cervical cancer screening clinics.
abstract: In France, cervical cancer screening is currently based on cytological examination of a Pap smear for women aged 25 to 65, but screening coverage is unsatisfactory. Previous studies have shown that self-sampling for human papillomavirus (HPV) testing increases rates of compliance. With this purpose in mind, we performed the CapU study to evaluate the acceptance of a urinary HPV test. Letters proposing a new cervical cancer screening method using at-home urine self-sampling were sent to 5000 women aged 40-65 years who had not had a Pap smear over the past three years. The participating patients had to send their urine samples to the Angers Hospital Virology Laboratory for analysis using real-time PCR. Of the 771 samples received, 687 were analyzed. High-risk HPV were detected in 29 women. In follow-up, 28 women with positive urinary HPV results had a Pap smear or colposcopy done. The cytological results showed nine abnormal Pap smears, among which histology studies confirmed three cases of cervical intraepithelial neoplasia grade III lesions. Our study shows that urinary HPV testing may be pertinent to women who do not have cervical Pap smears done and lead to the diagnosis of high-grade cervical lesions.
authors.affiliation: CHU d'Angers, Laboratoire de Virologie et Laboratoire HIFIH, UPRES EA 3859, 4, rue Larrey, 49000 Angers, France. Electronic address: alducancelle@chu-angers.fr.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Ducancelle
authors.affiliation: CHU d'Angers, Laboratoire de Virologie et Laboratoire HIFIH, UPRES EA 3859, 4, rue Larrey, 49000 Angers, France.
authors.forename: Justine
authors.initials: J
authors.lastname: Reiser
authors.affiliation: CHU d'Angers, Laboratoire de Virologie et Laboratoire HIFIH, UPRES EA 3859, 4, rue Larrey, 49000 Angers, France.
authors.forename: Adeline
authors.initials: A
authors.lastname: Pivert
authors.affiliation: CHU d'Angers, Laboratoire de Virologie et Laboratoire HIFIH, UPRES EA 3859, 4, rue Larrey, 49000 Angers, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Le Guillou-Guillemette
authors.affiliation: Cap Santé 49, Structure de Gestion des Cancers, 11, rue des Noyers, 49 000 Angers, France.
authors.forename: Anne Sophie
authors.initials: AS
authors.lastname: Le Duc-Banaszuk
authors.affiliation: CHU d'Angers, Laboratoire de Virologie et Laboratoire HIFIH, UPRES EA 3859, 4, rue Larrey, 49000 Angers, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Lunel-Fabiani
mesh_terms: D000328:Adult; D000368:Aged; D003127:Colposcopy; D004279:DNA, Viral / Q000652:urine*; D055088:Early Detection of Cancer / Q000379:methods*; D005260:Female; D005602:France; D061809:Human Papillomavirus DNA Tests; D006801:Humans; D008403:Mass Screening; D008875:Middle Aged; D065006:Papanicolaou Test; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis*; D010349:Patient Compliance; D011247:Pregnancy; D012648:Self Care; D012680:Sensitivity and Specificity; D013048:Specimen Handling; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis*
chemical_list: D004279:DNA, Viral
keywords: Cervical cancer screening; HPV DNA detection; HR HPV; High-Risk Human Papillomavirus; Self-sampling for HPV testing; Urine