title: Safety and feasibility of volumetric laser endomicroscopy in patients with Barrett's esophagus (with videos).
abstract: BACKGROUND
  Volumetric laser endomicroscopy (VLE) produces high-resolution, cross-sectional surface, and subsurface images for detecting neoplasia, targeting biopsies, and guiding real-time treatment.
  
  
  OBJECTIVE
  To evaluate the safety and feasibility of the Nvision VLE system.
  
  
  METHODS
  Prospective, multicenter study.
  
  
  METHODS
  Tertiary-care medical centers.
  
  
  METHODS
  One hundred patients with suspected Barrett's esophagus, including 52 patients with prior endotherapy.
  
  
  METHODS
  The first-generation Nvision VLE Imaging System, a balloon-centered, rotating optical probe provided images of the mucosa and submucosa through a 6-cm segment length and 360° scan of the distal esophagus.
  
  
  METHODS
  Acquisition of a complete, 6-cm scan from the distal esophagus, demographic and procedural data, and final histologic diagnosis.
  
  
  RESULTS
  VLE imaging was successfully performed in 87 cases. After VLE imaging, biopsy specimens were obtained in 77 patients and mucosal resection was performed in 20 patients. The final pathologic diagnoses of the patients studied were adenocarcinoma (4 patients), high-grade dysplasia (10 patients), low-grade dysplasia (11 patients), indefinite (5 patients), intestinal metaplasia (29 patients), and normal squamous cells (18 patients). VLE was not completed in 13 of 100 (13%) because of optical probe and console issues. There were 2 minor adverse events (mucosal lacerations not requiring therapy).
  
  
  CONCLUSIONS
  This was a feasibility study with a first-generation device. There was no direct histopathologic correlation with the VLE images or any comparative analysis with white-light endoscopy or narrow-band imaging findings.
  
  
  CONCLUSIONS
  VLE is a safe procedure for patients with suspected or confirmed Barrett's esophagus. Real-time VLE images enabled visualization of the mucosa and submucosa in 87% of cases. Further studies are needed to evaluate the in vivo diagnostic accuracy and clinical utility of VLE.
authors.affiliation: Department of Gastroenterology, Mayo Clinic, Jacksonville, Florida.
authors.forename: Herbert C
authors.initials: HC
authors.lastname: Wolfsen
authors.affiliation: Gastroenterology, Hepatology, and Motility, Kansas City Veterans Administration Medical Center, Kansas City, Missouri.
authors.forename: Prateek
authors.initials: P
authors.lastname: Sharma
authors.affiliation: Department of Gastroenterology, Mayo Clinic, Jacksonville, Florida.
authors.forename: Michael B
authors.initials: MB
authors.lastname: Wallace
authors.affiliation: Department of Gastroenterology, Mayo Clinic Rochester, Rochester, Minnesota.
authors.forename: Cadman
authors.initials: C
authors.lastname: Leggett
authors.affiliation: Department of Pathology, Massachusetts General Hospital, Boston, Massachusetts.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Tearney
authors.affiliation: Department of Gastroenterology, Mayo Clinic Rochester, Rochester, Minnesota.
authors.forename: Kenneth K
authors.initials: KK
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001471:Barrett Esophagus / Q000473:pathology* / Q000601:surgery; D001706:Biopsy; D004945:Esophagoscopy / Q000295:instrumentation* / Q000379:methods; D004947:Esophagus / Q000473:pathology* / Q000601:surgery; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009092:Mucous Membrane / Q000473:pathology / Q000601:surgery; D061214:Patient Safety; D011446:Prospective Studies; D041623:Tomography, Optical Coherence / Q000295:instrumentation* / Q000379:methods; D014741:Video Recording