title: [Knockdown of InR gene in ventral nephrocytes promotes resistance to toxic stress in Drosophila melanogaster females].
abstract: Hemolymph filtration in insects is performed by nephrocytes, additional cells of the circulatory system that are not connected to Malpighian vessels. Drosophila has two types of nephrocytes: the ventral ("garland"), which are situated around the connection site of the esophagus and proventriculus, and the pericardial, which are localized around the heart. In this study, we examined the role of the of insulin-like receptor (InR)gene in regulation of the function of ventral nephrocytes (VNC) in D. melanogaster females. Immunofluorescent analysis of female VNC with anti-InR antibodies revealed for the first time that the InR gene is expressed in VNC cells. To determine whether a change in the level of InR expression has an effect on VNC function in Drosophila females, we implemented an antisense suppressor of the InR gene, together with a driver that is expressed specifically in VNC. VNC function was evaluated by survival of the females exposed to toxic stress (treatment with AgNO3). This study has shown for the first time that suppression of InR expression in VNC leads to a rise in the survival of flies under conditions of toxic stress.
authors.forename: O V
authors.initials: OV
authors.lastname: Andreenkova
authors.forename: E K
authors.initials: EK
authors.lastname: Karpova
authors.forename: P N
authors.initials: PN
authors.lastname: Menshanov
authors.forename: I Yu
authors.initials: IY
authors.lastname: Rauschenbach
mesh_terms: D000818:Animals; D029721:Drosophila Proteins / Q000096:biosynthesis* / Q000235:genetics; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D004947:Esophagus / Q000502:physiology; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D055786:Gene Knockout Techniques; D006458:Hemolymph / Q000378:metabolism*; D007328:Insulin / Q000235:genetics / Q000378:metabolism; D011531:Proventriculus / Q000502:physiology; D034622:RNA Interference; D020794:Receptor Protein-Tyrosine Kinases / Q000096:biosynthesis* / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D012835:Silver Nitrate / Q000633:toxicity; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics*
chemical_list: D029721:Drosophila Proteins; D007328:Insulin; D012835:Silver Nitrate; C094982:InR protein, Drosophila; D020794:Receptor Protein-Tyrosine Kinases