title: Characterization of an inducible C2 H2 -type zinc finger transcription factor VuSTOP1 in rice bean (Vigna umbellata) reveals differential regulation between low pH and aluminum tolerance mechanisms.
abstract: The rice bean (Vigna umbellata) root apex specifically secretes citrate through expression activation of Vigna umbellata Multidrug and Toxic Compound Extrusion 1 (VuMATE1) under aluminum (Al(3+) ) stress. However, the underlying mechanisms regulating VuMATE1 expression remain unknown. We isolated and characterized a gene encoding Sensitive to Proton Rhizotoxicity1 (STOP1)-like protein, VuSTOP1, from rice bean. The role of VuSTOP1 in regulating VuMATE1 expression was investigated using the yeast one-hybrid assay. We characterized the function of VuSTOP1 in Al(3)  (+)  - and H(+) -tolerance using in planta complementation assays. We demonstrated that VuSTOP1 has transactivation potential. We found that VuSTOP1 expression is inducible by Al(3+) and H(+) stress. However, although VuSTOP1 binds to the promoter of VuMATE1, the inconsistent tissue localization patterns of VuSTOP1 and VuMATE1 preclude VuSTOP1 as the major factor regulating VuMATE1 expression. In addition, when a protein translation inhibitor increased expression of VuSTOP1, VuMATE1 expression was inhibited. In planta complementation assay demonstrated that VuSTOP1 could fully restore expression of genes involved in H(+) tolerance, but could only partially restore expression of AtMATE. We conclude that VuSTOP1 plays a major role in H(+) tolerance, but only a minor role in Al(3+) tolerance. The differential transcriptional regulation of VuSTOP1 and VuMATE1 reveals a complex regulatory system controlling VuMATE1 expression.
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.|College of Resources and Environment, Yunnan Agricultural University, Kunming, 650201, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.
authors.forename: He Qiang
authors.initials: HQ
authors.lastname: Lou
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Yu Long
authors.initials: YL
authors.lastname: Gong
authors.affiliation: Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, 310008, China.
authors.forename: Mei Ya
authors.initials: MY
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Meng Jie
authors.initials: MJ
authors.lastname: Cao
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Jian Li
authors.initials: JL
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Shao Jian
authors.initials: SJ
authors.lastname: Zheng
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects*; D000535:Aluminum / Q000633:toxicity*; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000502:physiology; D001483:Base Sequence; D003001:Cloning, Molecular; D003513:Cycloheximide / Q000494:pharmacology; D007887:Fabaceae / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D005816:Genetic Complementation Test; D005966:Glucuronidase / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011522:Protons; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D016415:Sequence Alignment; D013347:Subcellular Fractions / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation; D020798:Two-Hybrid System Techniques; D016335:Zinc Fingers
chemical_list: D010940:Plant Proteins; D011522:Protons; D012333:RNA, Messenger; D014157:Transcription Factors; D003513:Cycloheximide; D000535:Aluminum; D005966:Glucuronidase
keywords: Sensitive to Proton Rhizotoxicity1 (STOP1); aluminum (Al) toxicity; citrate secretion; proton toxicity; rice bean (Vigna umbellata); transcriptional regulation