title: Decreased IL-27 Negatively Correlated with Th17 Cells in Non-Small-Cell Lung Cancer Patients.
abstract: The presence of Th17 cells and IL-27 is observed in a variety of inflammatory associated cancers. However, there are some data on the role of Th17 cells and IL-27 in the regulation of immune reactions in non-small-cell lung cancer (NSCLC). The aim of this study is to assess the variation of Th17 cells and IL-27 in the peripheral blood (PB) of patients with NSCLC. The proportion of Th17 cells in peripheral blood mononuclear cells (PBMCs) was evaluated by flow cytometry. The serum concentrations of IL-27 and IL-17 were measured by enzyme-linked immunosorbent assay (ELISA). The mRNA expression of RORγt and IL-27 in the peripheral blood was examined by real-time quantitative polymerase chain reaction (QPCR). Expression of IL-27 was lower in NSCLC patients compared with normal controls. The frequency of Th17 cells was increased in NSCLC patients, accompanied by the upregulation of IL-17 and RORγt. IL-27 negatively correlated with the number of Th17 cells and the RORγt mRNA. Our results indicate that IL-27 might inhibit Th17 differentiation in NSCLC patients and better understanding of the regulatory effects of IL-27 on Th17 cells may shed light on potential new targets in cancer prevention and therapy.
authors.affiliation: Department of Respiratory Medicine, The Eighth People's Hospital of Nanning, Nanning, Guangxi 530001, China ; Department of Respiratory Medicine, The First People's Hospital of Nanning, Nanning, Guangxi 530001, China.
authors.forename: Minchao
authors.initials: M
authors.lastname: Duan
authors.affiliation: Department of Respiratory Medicine, The Eighth People's Hospital of Nanning, Nanning, Guangxi 530001, China.
authors.forename: Zhengqing
authors.initials: Z
authors.lastname: Ning
authors.affiliation: Department of Respiratory Medicine, The First People's Hospital of Nanning, Nanning, Guangxi 530001, China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Fu
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital, Guangxi Medical University, Nanning, Guangxi 530021, China.
authors.forename: Jianquan
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital, Guangxi Medical University, Nanning, Guangxi 530021, China.
authors.forename: Guangnan
authors.initials: G
authors.lastname: Liu
authors.affiliation: Department of Respiratory Medicine, The First People's Hospital of Nanning, Nanning, Guangxi 530001, China.
authors.forename: Qiu
authors.initials: Q
authors.lastname: Wei
authors.affiliation: Department of Respiratory Medicine, The First People's Hospital of Nanning, Nanning, Guangxi 530001, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Zheng
mesh_terms: D000328:Adult; D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood* / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D064094:Interleukin-27 / Q000097:blood*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D058504:Th17 Cells / Q000276:immunology*
chemical_list: D064094:Interleukin-27