title: Highly Amino Acid Selective Hydrolysis of Myoglobin at Aspartate Residues as Promoted by Zirconium(IV)-Substituted Polyoxometalates.
abstract: SDS-PAGE/Edman degradation and HPLC MS/MS showed that zirconium(IV)-substituted Lindqvist-, Keggin-, and Wells-Dawson-type polyoxometalates (POMs) selectively hydrolyze the protein myoglobin at Asp-X peptide bonds under mildly acidic and neutral conditions. This transformation is the first example of highly sequence selective protein hydrolysis by POMs, a novel class of protein-hydrolyzing agents. The selectivity is directed by Asp residues located on the surface of the protein and is further assisted by electrostatic interactions between the negatively charged POMs and positively charged surface patches in the vicinity of the cleavage site.
authors.affiliation: Department of Chemistry, KU Leuven, Celestijnenlaan 200F, 3001 Leuven (Belgium) http://www.chem.kuleuven.be/lbc/index.html.
authors.forename: Hong Giang T
authors.initials: HG
authors.lastname: Ly
authors.affiliation: Department of Chemistry, KU Leuven, Celestijnenlaan 200F, 3001 Leuven (Belgium) http://www.chem.kuleuven.be/lbc/index.html.
authors.forename: Gregory
authors.initials: G
authors.lastname: Absillis
authors.affiliation: Laboratory of Molecular Immunology, Rega Institute, Department of Microbiology and Immunology, KU Leuven, Minderbroedersstraat 10, 3000 Leuven (Belgium).
authors.forename: Rik
authors.initials: R
authors.lastname: Janssens
authors.affiliation: Laboratory of Molecular Immunology, Rega Institute, Department of Microbiology and Immunology, KU Leuven, Minderbroedersstraat 10, 3000 Leuven (Belgium).
authors.forename: Paul
authors.initials: P
authors.lastname: Proost
authors.affiliation: Department of Chemistry, KU Leuven, Celestijnenlaan 200F, 3001 Leuven (Belgium) http://www.chem.kuleuven.be/lbc/index.html. tatjana.vogt@chem.kuleuven.be.
authors.forename: Tatjana N
authors.initials: TN
authors.lastname: Parac-Vogt
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001224:Aspartic Acid / Q000737:chemistry*; D006736:Horses; D006868:Hydrolysis / Q000187:drug effects*; D008958:Models, Molecular; D009211:Myoglobin / Q000737:chemistry*; D017973:Tungsten Compounds / Q000737:chemistry / Q000494:pharmacology*; D015040:Zirconium / Q000737:chemistry / Q000494:pharmacology*; D000099294:Polyoxometalates
chemical_list: D001224:Aspartic Acid; D009211:Myoglobin; D017973:Tungsten Compounds; D015040:Zirconium; C102296:polyoxometalate I; D000099294:Polyoxometalates
keywords: homogeneous catalysis; horse-heart myoglobin; hydrolysis; metalloproteases; polyoxometalates