title: Detection of 3-phenoxybenzoic acid in river water with a colloidal gold-based lateral flow immunoassay.
abstract: 3-Phenoxybenzoic acid (3-PBA) is a general metabolite of synthetic pyrethroids. It could be used as a generic biomarker for multiple pyrethroids exposure for human or pyrethroid residues in the environment. In this study, monoclonal antibodies (mAbs) against 3-PBA were developed by using PBA-bovine serum albumin (BSA) as an immunogen. In the competitive enzyme-linked immunosorbent assay (ELISA) format, the I50 and I10 values of purified mAbs were 0.63 and 0.13 μg/ml, respectively, with a dynamic range between 0.19 and 2.04 μg/ml. Then, the colloidal gold (CG)-based lateral flow immunoassay was established based on the mAbs. The working concentration of coating antigen and CG-labeled antibodies and the blocking effects were investigated to get optimal assay performance. The cutoff value for the assay was 1 μg/ml 3-PBA, and the detection time was within 10 min. A total of 40 river water samples were spiked with 3-PBA at different levels and determined by the lateral flow immunoassay without any sample pretreatments. The negative false rate was 2.5%, and no positive false results were observed at these levels. This lateral flow immunoassay has the potential to be an on-site screening method for monitoring 3-PBA or pyrethroid residues in environmental samples.
authors.affiliation: College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; Key Laboratory of Food Quality and Safety of Jiangsu Province, Nanjing 210014, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; Key Laboratory of Food Quality and Safety of Jiangsu Province, Nanjing 210014, China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Wu
authors.affiliation: Key Laboratory of Food Quality and Safety of Jiangsu Province, Nanjing 210014, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Hu
authors.affiliation: Key Laboratory of Food Quality and Safety of Jiangsu Province, Nanjing 210014, China.
authors.forename: Manman
authors.initials: M
authors.lastname: Lin
authors.affiliation: Key Laboratory of Food Quality and Safety of Jiangsu Province, Nanjing 210014, China.
authors.forename: Mengtang
authors.initials: M
authors.lastname: Wen
authors.affiliation: Key Laboratory of Food Quality and Safety of Jiangsu Province, Nanjing 210014, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Food Quality and Safety of Jiangsu Province, Nanjing 210014, China.
authors.forename: Chongxin
authors.initials: C
authors.lastname: Xu
authors.affiliation: Key Laboratory of Food Quality and Safety of Jiangsu Province, Nanjing 210014, China.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Hu
authors.affiliation: Key Laboratory of Food Quality and Safety of Jiangsu Province, Nanjing 210014, China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Zhong
authors.affiliation: Key Laboratory of Food Quality and Safety of Jiangsu Province, Nanjing 210014, China.
authors.forename: Lingxia
authors.initials: L
authors.lastname: Jiao
authors.affiliation: Key Laboratory of Food Quality and Safety of Jiangsu Province, Nanjing 210014, China.
authors.forename: Yajing
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Key Laboratory of Food Quality and Safety of Jiangsu Province, Nanjing 210014, China.
authors.forename: Cunzhen
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Food Quality and Safety of Jiangsu Province, Nanjing 210014, China.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Yu
authors.affiliation: Key Laboratory of Food Quality and Safety of Jiangsu Province, Nanjing 210014, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liang
authors.affiliation: College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; Key Laboratory of Food Quality and Safety of Jiangsu Province, Nanjing 210014, China. Electronic address: liuyuan@jaas.ac.cn.
authors.forename: Xianjin
authors.initials: X
authors.lastname: Liu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D000941:Antigens / Q000378:metabolism; D001565:Benzoates / Q000032:analysis* / Q000737:chemistry; D002417:Cattle; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D017979:Gold Colloid / Q000737:chemistry*; D007118:Immunoassay / Q000379:methods*; D008567:Membranes, Artificial; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D011722:Pyrethrins / Q000737:chemistry; D045483:Rivers / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D001565:Benzoates; D017979:Gold Colloid; D008567:Membranes, Artificial; D011722:Pyrethrins; D014874:Water Pollutants, Chemical; C017618:3-phenoxybenzoic acid; D003101:Collodion
keywords: 3-Phenoxybenzoic acid; Colloidal gold; Lateral flow immunoassay; Monoclonal antibodies