title: A Systems Approach to Elucidate Heterosis of Protein Abundances in Yeast.
abstract: Heterosis is a universal phenomenon that has major implications in evolution and is of tremendous agro-economic value. To study the molecular manifestations of heterosis and to find factors that maximize its strength, we implemented a large-scale proteomic experiment in yeast. We analyzed the inheritance of 1,396 proteins in 55 inter- and intraspecific hybrids obtained from Saccharomyces cerevisiae and S. uvarum that were grown in grape juice at two temperatures. We showed that the proportion of heterotic proteins was highly variable depending on the parental strain and on the temperature considered. For intraspecific hybrids, this proportion was higher at nonoptimal temperature. Unexpectedly, heterosis for protein abundance was strongly biased toward positive values in interspecific hybrids but not in intraspecific hybrids. Computer modeling showed that this observation could be accounted for by assuming concave relationships between protein abundances and their controlling factors, in line with the metabolic model of heterosis. These results point to nonlinear processes that could play a central role in heterosis.
authors.affiliation: From the INRA, PAPPSO, UMR 0320/UMR 8120 Génétique Végétale, F-91190, Gif-sur-Yvette, France;
authors.forename: Mélisande
authors.initials: M
authors.lastname: Blein-Nicolas
authors.affiliation: CNRS, UMR 0320/UMR 8120, Génétique Végétale, F-91190 Gif-sur-Yvette, France; Université Bordeaux, Unité de Recherche Œnologie, EA 4577, ISVV, 210 chemin de Leysotte, 33140 Villenave d'Ornon, France;
authors.forename: Warren
authors.initials: W
authors.lastname: Albertin
authors.affiliation: From the INRA, PAPPSO, UMR 0320/UMR 8120 Génétique Végétale, F-91190, Gif-sur-Yvette, France; Ariana Pharmaceuticals, 28 rue du Docteur Finlay, 75015 Paris, France;
authors.forename: Telma
authors.initials: T
authors.lastname: da Silva
authors.affiliation: CNRS, Université de Franche-Comté, UMR 6249 Chrono-Environnement, F-25000, Besançon, France;
authors.forename: Benoît
authors.initials: B
authors.lastname: Valot
authors.affiliation: From the INRA, PAPPSO, UMR 0320/UMR 8120 Génétique Végétale, F-91190, Gif-sur-Yvette, France;
authors.forename: Thierry
authors.initials: T
authors.lastname: Balliau
authors.affiliation: Université Bordeaux, Unité de Recherche Œnologie, EA 4577, ISVV, 210 chemin de Leysotte, 33140 Villenave d'Ornon, France; Bordeaux Sciences Agro, Gradignan, France;
authors.forename: Isabelle
authors.initials: I
authors.lastname: Masneuf-Pomarède
authors.affiliation: Université Bordeaux, Unité de Recherche Œnologie, EA 4577, ISVV, 210 chemin de Leysotte, 33140 Villenave d'Ornon, France;
authors.forename: Marina
authors.initials: M
authors.lastname: Bely
authors.affiliation: Université Bordeaux, Unité de Recherche Œnologie, EA 4577, ISVV, 210 chemin de Leysotte, 33140 Villenave d'Ornon, France; BIOLAFFORT, F-33034 Bordeaux, France;
authors.forename: Philippe
authors.initials: P
authors.lastname: Marullo
authors.affiliation: Univ Paris-Sud, UMR 0320/UMR 8120 Génétique Végétale, F-91190, Gif-sur-Yvette, France; INRA, UMR1083, 2 Place Viala, F-34060 Montpellier, France;
authors.forename: Delphine
authors.initials: D
authors.lastname: Sicard
authors.affiliation: Univ Paris-Sud, UMR 0320/UMR 8120 Génétique Végétale, F-91190, Gif-sur-Yvette, France;
authors.forename: Christine
authors.initials: C
authors.lastname: Dillmann
authors.affiliation: Univ Paris-Sud, UMR 0320/UMR 8120 Génétique Végétale, F-91190, Gif-sur-Yvette, France;
authors.forename: Dominique
authors.initials: D
authors.lastname: de Vienne
authors.affiliation: CNRS, PAPPSO, UMR 0320/UMR 8120 Génétique Végétale, F-91190, Gif-sur-Yvette, France zivy@moulon.inra.fr.
authors.forename: Michel
authors.initials: M
authors.lastname: Zivy
mesh_terms: D002853:Chromatography, Liquid; D003198:Computer Simulation; D018506:Gene Expression Regulation, Plant; D006823:Hybrid Vigor; D006824:Hybridization, Genetic; D017711:Nonlinear Dynamics; D025341:Principal Component Analysis; D020543:Proteome / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D013045:Species Specificity; D053719:Tandem Mass Spectrometry; D013696:Temperature; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D020543:Proteome; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors