title: Histone deacetylase 3 inhibits new tumor suppressor gene DTWD1 in gastric cancer.
abstract: Cancer epigenetics plays an important role in the pathogenesis of many cancers including gastric cancer. Histone deacetylases (HDACs) emerge as exciting therapeutic targets for cancer treatment and prevention. In this study, we identified DTWD1 as one of the 122 genes upregulated after treatment of trichostatin A (TSA) in two gastric cancer cell lines. Moreover, DTWD1 was downregulated in gastric cancer cell lines and primary gastric carcinoma tissues. It was further identified as the new target of p53. Then we revealed that HDAC3 downregulated DTWD1 by disrupting the interaction of p53 with DTWD1 promoter. Furthermore, DTWD1 functioned as a tumor suppressor by downregulating cyclin B1 expression to inhibit proliferation. In summary, as the new p53 target gene, DTWD1 was downregulated in gastric cancer by HDAC3 and acted as a novel tumor suppressor gene. Specific inhibitors of HDAC3 might be a new approach for gastric cancer treatment by activating DTWD1 expression.
authors.affiliation: Laboratory of Cancer Biology, Department of Medical Oncology, Key laboratory of Biotherapy in Zhejiang Province, Sir Runrun Shaw Hospital Zhejiang University, China.
authors.forename: Yanning
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Laboratory of Cancer Biology, Department of Medical Oncology, Key laboratory of Biotherapy in Zhejiang Province, Sir Runrun Shaw Hospital Zhejiang University, China.
authors.forename: Yongfang
authors.initials: Y
authors.lastname: Yue
authors.affiliation: Laboratory of Cancer Biology, Department of Medical Oncology, Key laboratory of Biotherapy in Zhejiang Province, Sir Runrun Shaw Hospital Zhejiang University, China.
authors.forename: Min
authors.initials: M
authors.lastname: Pan
authors.affiliation: Laboratory of Cancer Biology, Department of Medical Oncology, Key laboratory of Biotherapy in Zhejiang Province, Sir Runrun Shaw Hospital Zhejiang University, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Sun
authors.affiliation: Laboratory of Cancer Biology, Department of Medical Oncology, Key laboratory of Biotherapy in Zhejiang Province, Sir Runrun Shaw Hospital Zhejiang University, China.
authors.forename: Jue
authors.initials: J
authors.lastname: Chu
authors.affiliation: Laboratory of Cancer Biology, Department of Medical Oncology, Key laboratory of Biotherapy in Zhejiang Province, Sir Runrun Shaw Hospital Zhejiang University, China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Lin
authors.affiliation: Laboratory of Cancer Biology, Department of Medical Oncology, Key laboratory of Biotherapy in Zhejiang Province, Sir Runrun Shaw Hospital Zhejiang University, China.
authors.forename: Wenxia
authors.initials: W
authors.lastname: Xu
authors.affiliation: Laboratory of Cancer Biology, Department of Medical Oncology, Key laboratory of Biotherapy in Zhejiang Province, Sir Runrun Shaw Hospital Zhejiang University, China.
authors.forename: Lifeng
authors.initials: L
authors.lastname: Feng
authors.affiliation: Departrment of Gastroenterology, The 2nd Hospital of Zhejiang University China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Laboratory of Cancer Biology, Department of Medical Oncology, Key laboratory of Biotherapy in Zhejiang Province, Sir Runrun Shaw Hospital Zhejiang University, China.
authors.forename: Dingwei
authors.initials: D
authors.lastname: Chen
authors.affiliation: Departrment of Surgery, The University of Hong Kong China.
authors.forename: Vivian Y
authors.initials: VY
authors.lastname: Shin
authors.affiliation: Laboratory of Cancer Biology, Department of Medical Oncology, Key laboratory of Biotherapy in Zhejiang Province, Sir Runrun Shaw Hospital Zhejiang University, China.
authors.forename: Xian
authors.initials: X
authors.lastname: Wang
authors.affiliation: Laboratory of Cancer Biology, Department of Medical Oncology, Key laboratory of Biotherapy in Zhejiang Province, Sir Runrun Shaw Hospital Zhejiang University, China.
authors.forename: Hongchuan
authors.initials: H
authors.lastname: Jin
keywords: DTWD1; HDAC; gastric cancer; p53; tumor suppressor gene