title: Desulfurization of immobilized sulfur-oxidizing bacteria, Thialkalivibrio versutus, by magnetic nanaoparticles under haloalkaliphilic conditions.
abstract: OBJECTIVE
  As a haloalkaliphilic, sulfur-oxidizing bacteria, Thialkalivibrio versutus D301 can remove sulfide, thiosulfate and polysulfide in wastewater, we investigated how it might be reused when mixed with high concentrations of elemental sulfur.
  
  
  RESULTS
  A process is described to immobilize T. versutus cells by using superparamagnetic Fe3O4 nanoparticles (NPs) under haloalkaliphilic conditions (i.e. pH 9.5, 0.5 M Na(+)). The saturation magnetization value (δs) of immobilized cells was 55.1 emu/g. The Fe3O4 NPs-coated cells had the similar sulfur oxidization activity to that of free cells, and they could be reused six batch cycles. Analysis of hydraulic diameters showed that bacterial cells were immobilized by Fe3O4 NPs due to the nano-size effects.
  
  
  CONCLUSIONS
  Magnetic immobilization is a convenient technique for cell immobilization under haloalkaliphilic conditions and is a promising technology for large scale application.
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, People's Republic of China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Xu
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Cai
authors.forename: Ziyu
authors.initials: Z
authors.lastname: Song
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Qiu
authors.forename: Jiemin
authors.initials: J
authors.lastname: Zhou
authors.forename: Yilan
authors.initials: Y
authors.lastname: Liu
authors.forename: Tingzhen
authors.initials: T
authors.lastname: Mu
authors.forename: Dan
authors.initials: D
authors.lastname: Wu
authors.forename: Yueping
authors.initials: Y
authors.lastname: Guan
authors.forename: Jianmin
authors.initials: J
authors.lastname: Xing
mesh_terms: D018914:Cells, Immobilized / Q000378:metabolism*; D042001:Ectothiorhodospiraceae / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008280:Magnetics; D053758:Nanoparticles; D013455:Sulfur / Q000378:metabolism*; D013457:Sulfur Compounds / Q000378:metabolism*
chemical_list: D013457:Sulfur Compounds; D013455:Sulfur