title: Global profiling of histone modifications in the polyomavirus BK virion minichromosome.
abstract: During polyomavirus infection, the viral DNA adopts histones from host cells and forms minichromosomes as an important part of the viral life cycle. However, the detailed mechanisms of this histone incorporation remain unclear. Here, we profiled the histone posttranslational modifications (PTMs) in BKPyV minichromosomes and in the chromatin of BKPyV host cells. Through Triton-acetic acid-urea (TAU)-PAGE separation followed by nanoflow liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) analysis, we identified different kinds of PTMs on histones from BKPyV minichromosomes and from host cells. We observed not only the common PTMs on histones such as acetylation, methylation, phosphorylation, ubiquitination, and formylation but also several novel PTM sites. Our results also confirmed that the BKPyV minichromosome is hyperacetylated. Our detailed histone PTM profiles for the BKPyV minichromosome provide insights for future exploration of the underlying mechanisms and biological relevance of these histone PTMs.
authors.affiliation: Department of Medical Research, Chia-Yi Christian Hospital, Chia-Yi, Taiwan.
authors.forename: Chiung-Yao
authors.initials: CY
authors.lastname: Fang
authors.affiliation: Department of Urology, Chia-Yi Christian Hospital, Chia-Yi, Taiwan.
authors.forename: Cheng-Huang
authors.initials: CH
authors.lastname: Shen
authors.affiliation: Department of Microbiology and Immunology, Chung Shan Medical University, Taichung, Taiwan.
authors.forename: Meilin
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Medical Laboratory Science and Biotechnology, Central Taiwan University of Science and Technology, Taichung, Taiwan.
authors.forename: Pei-Lain
authors.initials: PL
authors.lastname: Chen
authors.affiliation: Institute of Molecular Biology, National Chung Cheng University, 168 University Rd., Min-Hsiung, Chia-Yi 621, Taiwan.
authors.forename: Michael W Y
authors.initials: MW
authors.lastname: Chan
authors.affiliation: Department of Bioscience and Biotechnology, National Taiwan Ocean University, 2 Pei-Ning Rd., Keelung 202, Taiwan. Electronic address: phsu@ntou.edu.tw.
authors.forename: Pang-Hung
authors.initials: PH
authors.lastname: Hsu
authors.affiliation: Institute of Molecular Biology, National Chung Cheng University, 168 University Rd., Min-Hsiung, Chia-Yi 621, Taiwan. Electronic address: biodcc@ccu.edu.tw.
authors.forename: Deching
authors.initials: D
authors.lastname: Chang
mesh_terms: D002853:Chromatography, Liquid; D004279:DNA, Viral / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000737:chemistry* / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D011120:Polyomavirus / Q000235:genetics / Q000502:physiology*; D011499:Protein Processing, Post-Translational; D053719:Tandem Mass Spectrometry
chemical_list: D004279:DNA, Viral; D006657:Histones
keywords: BKPyV; Histone modification; LC−MS/MS; Minichromosome; Nucleosome; Proteomics