title: Decreased level of phosphatidylcholine (16:0/20:4) in multiple myeloma cells compared to plasma cells: a single-cell MALDI-IMS approach.
abstract: Lipid metabolic changes under diseased conditions, particularly in solid tumors, are attracting increased attention. However, in non-solid tumors, including most hematopoietic tumors, lipid analyses are scarce. Multiple myeloma (MM) is a plasma cell disorder arising from bone marrow, and the lipid status of MM cells has not been reported yet. In this study, we analyzed flow cytometry-sorted single MM cells and normal plasma cells (NPCs) using matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS), a two-dimensional label-free mass spectrometry technique for biomolecular analysis, to obtain specific lipid information. We isolated 1.31-5.77% of MM cells and 0.03-0.24% of NPCs using fluorescence-activated cell sorting (FACS). Analysis of purified cells using MALDI-IMS at the single-cell level revealed that the peak intensity and ion signals of phosphatidylcholine [PC (16:0/20:4) + H](+) at m/z 782.5 were significantly decreased in MM cells compared to NPCs. By examining particular cell populations rather than cell mixtures, our method can become a suitable tool for the analysis of rare cell populations at the single-cell level and advance the understanding of MM progression.
authors.affiliation: Department of Cell Biology and Anatomy, Hamamatsu University School of Medicine, 1-20-1, Handayama, Higashi-ku, Hamamatsu, Shizuoka, 431-3192, Japan.
authors.forename: Md Amir
authors.initials: MA
authors.lastname: Hossen
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Nagata
authors.forename: Michihiko
authors.initials: M
authors.lastname: Waki
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Ide
authors.forename: Shiro
authors.initials: S
authors.lastname: Takei
authors.forename: Hana
authors.initials: H
authors.lastname: Fukano
authors.forename: Gustavo A
authors.initials: GA
authors.lastname: Romero-Perez
authors.forename: Shogo
authors.initials: S
authors.lastname: Tajima
authors.forename: Ikuko
authors.initials: I
authors.lastname: Yao
authors.forename: Kazunori
authors.initials: K
authors.lastname: Ohnishi
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Setou
mesh_terms: D045744:Cell Line, Tumor; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D006801:Humans; D009101:Multiple Myeloma / Q000737:chemistry* / Q000473:pathology*; D010713:Phosphatidylcholines / Q000032:analysis*; D010950:Plasma Cells / Q000737:chemistry*; D059010:Single-Cell Analysis / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014407:Tumor Cells, Cultured
chemical_list: D010713:Phosphatidylcholines