title: Antibacterial activity and mutagenesis of sponge-associated Pseudomonas fluorescens H41.
abstract: Marine sponges (phylum Porifera) are well known to harbour a complex and diverse bacterial community. Some of these sponge-associated bacteria have been shown to be the real producers of secondary metabolites with a wide range of activities from antimicrobials to anticancer agents. Previously, we revealed that the strain Pseudomonas fluorescens H41 isolated from the sponge Haliclona sp. (collected at the coast of Rio de Janeiro, Brazil) showed a strong antimicrobial activity against clinical and marine bacteria. Thus, in this study the genes involved in the antimicrobial activity of P. fluorescens H41 were identified. To this end, a library of mutants was generated via miniTnphoA3 transposon mutagenesis and the resulting clones were characterized for their antimicrobial activity. It was demonstrated that genes involved in the biosynthesis of the pyoverdine siderophore are related to the inhibitory activity of P. fluorescens H41. Therefore, this strain might play an important role in the biocontrol of the host sponge.
authors.affiliation: Department of Bioengineering Sciences, Research Group of Microbiology and VIB, Vrije Universiteit Brussel (VUB), Brussels, Belgium.
authors.forename: Lumeng
authors.initials: L
authors.lastname: Ye
authors.forename: Juliana F
authors.initials: JF
authors.lastname: Santos-Gandelman
authors.forename: Cristiane C P
authors.initials: CC
authors.lastname: Hardoim
authors.forename: Isabelle
authors.initials: I
authors.lastname: George
authors.forename: Pierre
authors.initials: P
authors.lastname: Cornelis
authors.forename: Marinella S
authors.initials: MS
authors.lastname: Laport
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001938:Brazil; D004251:DNA Transposable Elements; D047129:Haliclona / Q000382:microbiology*; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D016254:Mutagenesis, Insertional; D009842:Oligopeptides / Q000378:metabolism; D011551:Pseudomonas fluorescens / Q000235:genetics* / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D009842:Oligopeptides; C042453:pyoverdin