title: [Characterization of hemodynamic ex vivo model to study endothelial activation by TNF-α in prefunded human veins].
abstract: Inflammation is recognized as part of the etiology of numerous diseases. The interaction among cells of the immunological system with local cells and molecules, such as cytokines and chemokines, allows cellular activation and response amplification. The importance of several physicochemical factors like frictional force, vascular flow, shear stress, and pressure is now recognized because they are known to modulate genetic expression and endothelial activation; however, there are very few studies that recreate such cellular microenvironments. Hence, it is of paramount importance to develop new models that will mimic physiological conditions. Our aim was to improve a human vein ex vivo model that would allow endothelial activation in flow conditions, to study the molecular components during adhesion, taking into consideration physicochemical parameters such as flow and shear stress. Endothelial umbilical human vein was used and activated with TNF-a in order to determine U937 monocytic cells adhesion, as well as cytokines secretion and ICAM-1 expression. This model will allow leukocyte adhesion studies, using different inflammatory stimulus, along with the signaling pathways involved in several pathologies.
authors.affiliation: Departamento de Ciencias de la Salud, DCBS, Universidad Autónoma Metropolitana Iztapalapa. México D.F., Mexico.|Laboratorio de Líquido Sinovial, Instituto Nacional de Rehabilitación, México, D.F., Mexico.|Unidad de Bioquímica, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, México, D.F., Mexico.|Posgrado en Biología Experimental, UAMI, México, D.F., Mexico.
authors.forename: Ambar
authors.initials: A
authors.lastname: López-Macay
authors.affiliation: Programa Institucional de Cáncer de Mama, Departamento de Medicina Genómica y Toxicología Ambiental. IIB, UNAM, México, D.F., Mexico.
authors.forename: Edgar Josúe
authors.initials: EJ
authors.lastname: Ruiz-Medina
authors.affiliation: Programa Institucional de Cáncer de Mama, Departamento de Medicina Genómica y Toxicología Ambiental. IIB, UNAM, México, D.F., Mexico.|Unidad de Bioquímica, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, México, D.F., Mexico.
authors.forename: José Luis
authors.initials: JL
authors.lastname: Ventura-Gallegos
authors.affiliation: Unidad de investigación Médica en Medicina Reproductiva, Hospital de Gineco-obstetricia n. 4 "Dr. Rafael Castelazo Ayala ", IMSS, México, D.F., Mexico.
authors.forename: Fabián
authors.initials: F
authors.lastname: Arechavaleta-Velasco
authors.affiliation: Departamento de Ciencias de la Salud, DCBS, Universidad Autónoma Metropolitana Iztapalapa. México D.F., Mexico.
authors.forename: Luis Enrique
authors.initials: LE
authors.lastname: Gómez-Quiroz
authors.affiliation: Departamento de Ciencias de la Salud, DCBS, Universidad Autónoma Metropolitana Iztapalapa. México D.F., Mexico.
authors.forename: Mina
authors.initials: M
authors.lastname: Konigsberg-Fainstein
authors.affiliation: Programa Institucional de Cáncer de Mama, Departamento de Medicina Genómica y Toxicología Ambiental. IIB, UNAM, México, D.F., Mexico.|Unidad de Bioquímica, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, México, D.F., Mexico.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Zentella-Dehesa
mesh_terms: D004730:Endothelium, Vascular / Q000502:physiology*; D006439:Hemodynamics; D006801:Humans; D066298:In Vitro Techniques; D008955:Models, Cardiovascular; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology*; D014471:Umbilical Veins
chemical_list: D014409:Tumor Necrosis Factor-alpha