title: Genetic association study of coronary collateral circulation in patients with coronary artery disease using 22 single nucleotide polymorphisms corresponding to 10 genes involved in postischemic neovascularization.
abstract: BACKGROUND
  Collateral growth in patients with coronary artery disease (CAD) is highly heterogeneous. Although multiple factors are thought to play a role in collateral development, the contribution of genetic factors to coronary collateral circulation (CCC) is largely unknown. The goal of this study was to assess whether functional single nucleotide polymorphisms (SNPs) in genes involved in vascular growth are associated with CCC.
  
  
  METHODS
  677 consecutive CAD patients were enrolled in the study and their CCC was assessed by the Rentrop method. 22 SNPs corresponding to 10 genes involved in postischemic neovascularization were genotyped and multivariate logistic regression models were adjusted using clinically relevant variables to estimate odds ratios and used to examine associations of allelic variants, genotypes and haplotypes with CCC.
  
  
  RESULTS
  Statistical analysis showed that the HIF1A rs11549465 and rs2057482; VEGFA rs2010963, rs1570360, rs699947, rs3025039 and rs833061; KDR rs1870377, rs2305948 and rs2071559; CCL2 rs1024611, rs1024610, rs2857657 and rs2857654; NOS3 rs1799983; ICAM1 rs5498 and rs3093030; TGFB1 rs1800469; CD53 rs6679497; POSTN rs3829365 and rs1028728; and LGALS2 rs7291467 polymorphisms, as well as their haplotype combinations, were not associated with CCC (p < 0.05).
  
  
  CONCLUSIONS
  We could not validate in our cohort the association of the NOS3 rs1799983, HIF1A rs11549465, VEGFA rs2010963 and rs699947, and LGALS2 rs7291467 variants with CCC reported by other authors. A validated SNP-based genome-wide association study is required to identify polymorphisms influencing CCC.
authors.affiliation: Unitat d'Anatomia i Embriologia Humanes, Departament de Patologia i Terapèutica Experimental, Facultat de Medicina, Campus de Ciències de la Salut de Bellvitge, Universitat de Barcelona, L'Hospitalet de Llobregat, Barcelona, 08907, Spain. joanduranf@ub.edu.
authors.forename: Joan
authors.initials: J
authors.lastname: Duran
authors.affiliation: Unitat d'Anatomia i Embriologia Humanes, Departament de Patologia i Terapèutica Experimental, Facultat de Medicina, Campus de Ciències de la Salut de Bellvitge, Universitat de Barcelona, L'Hospitalet de Llobregat, Barcelona, 08907, Spain. mariapilar.sanchez@uv.cl.|Departamento de Estadística, Facultad de Ciencias, Universidad de Valparaíso, Valparaíso, Chile. mariapilar.sanchez@uv.cl.
authors.forename: Pilar Sánchez
authors.initials: PS
authors.lastname: Olavarría
authors.affiliation: Unitat d'Anatomia i Embriologia Humanes, Departament de Patologia i Terapèutica Experimental, Facultat de Medicina, Campus de Ciències de la Salut de Bellvitge, Universitat de Barcelona, L'Hospitalet de Llobregat, Barcelona, 08907, Spain. mmola@imim.es.|Neurovascular Research Group (NEUVAS), Institut Municipal d'Investigació Mèdica. Hospital del Mar, PRBB, Parc de Recerca Biomèdica de Barcelona, Barcelona, Spain. mmola@imim.es.
authors.forename: Marina
authors.initials: M
authors.lastname: Mola
authors.affiliation: Unitat d'Anatomia i Embriologia Humanes, Departament de Patologia i Terapèutica Experimental, Facultat de Medicina, Campus de Ciències de la Salut de Bellvitge, Universitat de Barcelona, L'Hospitalet de Llobregat, Barcelona, 08907, Spain. vgotzens@ub.edu.
authors.forename: Víctor
authors.initials: V
authors.lastname: Götzens
authors.affiliation: Department of Cardiology and Hemodynamics, Centre Cardiovascular Sant Jordi, Barcelona, Spain. jcgllo@wanadoo.es.
authors.forename: Julio
authors.initials: J
authors.lastname: Carballo
authors.affiliation: Department of Cardiology and Hemodynamics, Centre Cardiovascular Sant Jordi, Barcelona, Spain. emartinp76@hotmail.com.
authors.forename: Eva Martín
authors.initials: EM
authors.lastname: Pelegrina
authors.affiliation: Department of Cardiology and Hemodynamics, Centre Cardiovascular Sant Jordi, Barcelona, Spain. 4021mpg@comb.cat.
authors.forename: Màrius
authors.initials: M
authors.lastname: Petit
authors.affiliation: Department of Cardiology, Hospital Mútua de Terrassa, Terrassa, Barcelona, Spain. uzarasl@yahoo.es.
authors.forename: Omar
authors.initials: O
authors.lastname: Abdul-Jawad
authors.affiliation: Department of Cardiology, Hospital Universitari Vall d'Hebron, Barcelona, Spain. iotaegui@vhebron.net.
authors.forename: Imanol
authors.initials: I
authors.lastname: Otaegui
authors.affiliation: Department of Cardiology, Hospital Universitari Vall d'Hebron, Barcelona, Spain. brugarci@vhebron.net.
authors.forename: Bruno García
authors.initials: BG
authors.lastname: del Blanco
authors.affiliation: Department of Cardiology, Hospital Universitari Vall d'Hebron, Barcelona, Spain. dgdorado@vhebron.net.
authors.forename: David
authors.initials: D
authors.lastname: García-Dorado
authors.affiliation: Departament of Morphological Sciences, Universitat Autònoma de Barcelona, Bellaterra, Cerdanyola del Vallès, Barcelona, Spain. josep.reig@uab.cat.
authors.forename: Josep
authors.initials: J
authors.lastname: Reig
authors.affiliation: Unitat d'Anatomia i Embriologia Humanes, Departament de Patologia i Terapèutica Experimental, Facultat de Medicina, Campus de Ciències de la Salut de Bellvitge, Universitat de Barcelona, L'Hospitalet de Llobregat, Barcelona, 08907, Spain. acordero@idibell.cat.|Àrea Epigenetics and Cancer Biology Area, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL), L'Hospitalet de Llobregat, Barcelona, Spain. acordero@idibell.cat.
authors.forename: Alex
authors.initials: A
authors.lastname: Cordero
authors.affiliation: Unitat d'Anatomia i Embriologia Humanes, Departament de Patologia i Terapèutica Experimental, Facultat de Medicina, Campus de Ciències de la Salut de Bellvitge, Universitat de Barcelona, L'Hospitalet de Llobregat, Barcelona, 08907, Spain. janta@ub.edu.
authors.forename: Josep Maria
authors.initials: JM
authors.lastname: de Anta
mesh_terms: D000368:Aged; D003097:Collateral Circulation; D003324:Coronary Artery Disease / Q000235:genetics* / Q000503:physiopathology*; D003326:Coronary Circulation; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D018919:Neovascularization, Physiologic; D020641:Polymorphism, Single Nucleotide