title: Current radiological techniques used to evaluate unilateral partial ureteral obstruction: an experimental rabbit study.
abstract: OBJECTIVE
  The aim of this study was to evaluate functional and prognostic benefits of Doppler ultrasonography (DU), diuretic renal scintigraphy (DRS), and magnetic resonance urography (MRU) during diagnosis and follow-up of ureteropelvic junction obstruction (UPJO) and to examine apoptosis rates caused by UPJO in an experimental rabbit model.
  
  
  METHODS
  Twenty-four rabbits were divided randomly into two groups. The left kidneys of 15 rabbits from the first group underwent Ulm-Miller surgery to create UPJO, whereas the left kidneys of nine rabbits from the second group underwent sham surgery. A pressure flow study (Whitaker's test) was done during postoperative week 6. Based on the Whitaker test, the DU, DRS, and MRU findings were compared. The number of apoptotic renal cells was counted after death.
  
  
  RESULTS
  The Whitaker test run during postoperative week 6 revealed obstructions in 15 rabbits from group 1; the nine rabbits of the sham group had no obstructions. Sensitivity and specificity of DRS were 93.3 and 88.8 %, respectively, and those of MRU were 93.3 and 88.8 %, respectively. The postoperative mean RI values were significantly higher than the preoperative values, associated with sensitivity of 86.6 % and specificity of 77.5 % for detecting UPJO. DRS, MRU, and RI could not predict UPJO in one (8 %), one (8 %), and two (16 %) kidneys, respectively. Likelihood ratio (LR) was 8.4 for MRU and scintigraphy, while for RI, LR was 3.9. Pathology specimens revealed that all kidneys with UPJO underwent apoptosis, and the number of apoptotic cells was significantly higher on the UPJO-created side than on the contralateral and in the sham group (p < 0.05). No test predicted all apoptosis related to UPJO.
  
  
  CONCLUSIONS
  The RI, DRS, and DMRU results correlated with the pressure flow results for detecting UPJO. No single radiological technique predicted all initial UPJO-created kidneys that concluded with apoptosis. Further studies are required to seek with better methods for diagnosing an obstruction or to define a combination of radiological techniques aiding in the management decision.
authors.affiliation: Department of Pediatric Surgery, Kanuni Sultan Süleyman Education and Research Hospital, 34300, Istanbul, Turkey, mehmetyaz@gmail.com.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Yazıcı
authors.forename: Suleyman
authors.initials: S
authors.lastname: Celebi
authors.forename: Özgür
authors.initials: Ö
authors.lastname: Kuzdan
authors.forename: Hüseyin
authors.initials: H
authors.lastname: Koçan
authors.forename: Halil Suat
authors.initials: HS
authors.lastname: Ayyıldız
authors.forename: İlkay Koray
authors.initials: İK
authors.lastname: Bayrak
authors.forename: Meltem Ceyhan
authors.initials: MC
authors.lastname: Bilgici
authors.forename: Oktay
authors.initials: O
authors.lastname: Yapıcı
authors.forename: Mehmet
authors.initials: M
authors.lastname: Kefeli
authors.forename: Ender
authors.initials: E
authors.lastname: Arıtürk
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D004195:Disease Models, Animal; D004232:Diuretics / Q000494:pharmacology; D006869:Hydronephrosis / Q000175:diagnosis / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D007677:Kidney Function Tests; D008279:Magnetic Resonance Imaging / Q000379:methods; D011817:Rabbits; D011877:Radionuclide Imaging / Q000379:methods; D012680:Sensitivity and Specificity; D018608:Ultrasonography, Doppler / Q000379:methods; D014517:Ureteral Obstruction / Q000150:complications / Q000175:diagnosis / Q000378:metabolism; D014567:Urography / Q000379:methods
chemical_list: D004232:Diuretics