title: Development of novel microsatellite markers for conservation genetic studies of Vulpes vulpes (Canidae) by using next-generation sequencing method.
abstract: The red fox, Vulpes vulpes (Canidae), is the most widely distributed terrestrial carnivore worldwide, but this species is classified as endangered in Korea. In this study, we developed 25 polymorphic microsatellite markers that included 3-13 (mean = 6.32) alleles per locus using 22 red fox individuals. The most polymorphic locus was FR(59)TG (13 alleles) and the least polymorphic loci were FR(70)TG and FR(182)AG (3 alleles each). No significant deviation from Hardy-Weinberg equilibrium (P < 0.05) was observed for the 25 markers. Observed (HO) and expected (HE) heterozygosity varied from 0.182 to 1.000 and from 0.175 to 0.929, respectively. These newly developed microsatellite markers will be useful for investigating the genetic diversity and population genetic structure of V. vulpes and will aid in developing conservation strategies for this species.
authors.affiliation: National Institute of Biological Resources, Environmental Research Complex, Incheon, Korea.
authors.forename: J-N
authors.initials: JN
authors.lastname: Yu
authors.affiliation: Species Restoration Technology Institute, Korea National Park Service, Yeongju, Korea.
authors.forename: C-U
authors.initials: CU
authors.lastname: Chung
authors.affiliation: National Institute of Biological Resources, Environmental Research Complex, Incheon, Korea.
authors.forename: K H
authors.initials: KH
authors.lastname: Oh
authors.affiliation: Species Restoration Technology Institute, Korea National Park Service, Gurye-gun, Korea.
authors.forename: B-K
authors.initials: BK
authors.lastname: Lee
authors.affiliation: National Institute of Biological Resources, Environmental Research Complex, Incheon, Korea Chaelim@korea.kr.
authors.forename: C E
authors.initials: CE
authors.lastname: Lim
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D003247:Conservation of Natural Resources; D056727:Endangered Species; D005589:Foxes / Q000235:genetics*; D014644:Genetic Variation; D005828:Genetics, Population; D006579:Heterozygote; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods / Q000662:veterinary*; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D056910:Republic of Korea; D017422:Sequence Analysis, DNA / Q000662:veterinary