title: Diphlorethohydroxycarmalol of Ishige okamurae and Caffeine Modified the Expression of Extracellular Fibrillars during Adipogenesis of Mouse Subcutaneous Adipose Derived Stem Cell.
abstract: Although, one of the etiologies of localized lipodystrophy of the subcutaneous connective tissue (cellulite) is the histological alternation of adipose tissue, the characteristics of expression of the components of extracellular matrix (ECM) components during adipogenesis are not uncovered. In this study, the effects of caffeine and Ishige okamurae originated diphlorethohydroxycarmalol (DPHC) on the expression of extracellualr fibers was analyzed with quantitative RT-PCR during differentiation induction of mouse subcutaneous adipose derived stem cells (msADSC) into adipocyte. The expression levels of Col1a, Col3a1, and Col61a were decreased by the adipogenci induction in a time-dependent manners. However, Col2a mRNA and Col4a1 mRNA expressions were oposit to them. Caffeine and DPHC stimulated the changes of the expression of these collagens. Eln mRNA expression was increased by induction. DPHC stimulated the expression of it. Mfap5 mRNA expression was deceased in both adipogenic cell and matured adipocytes. Caffeine suppressed the expression of Mfap5 but the effect of DPHC was different by the concentration. The expression of bioglycan, decorin, and lumican were also modified by caffeine and DPHC in a concentration-dependent manner. Based on this study, we revealed firstly the effects of caffeine and DPHC on the expression of collagens, elastin, and glycoproteins during adipogenesis of msADSCs. Those results suggest that DPHC may have antiadipogenic effect and has more positive effets on normal adipose tissue generation and work as suppressor the abnormality of ECM structure. Such results indicate that DPHC can be applied in keeping the stability of the ECM of adipogenic tissues.
authors.affiliation: Division of Developmental Biology and Physiology, School of Biosicences and Chemistry, Sungshin Women's University, Seoul 142-742, Republic of Korea.
authors.forename: Younmi
authors.initials: Y
authors.lastname: Jeon
authors.affiliation: NSTECH Co. Ltd., Incheon 405-848, Republic of Korea.
authors.forename: Siyoung
authors.initials: S
authors.lastname: Song
authors.affiliation: Seojin BioTech Co. Ltd., Suwon 443-373, Republic of Korea.
authors.forename: Hagju
authors.initials: H
authors.lastname: Kim
authors.affiliation: Division of Developmental Biology and Physiology, School of Biosicences and Chemistry, Sungshin Women's University, Seoul 142-742, Republic of Korea.
authors.forename: Yong-Pil
authors.initials: YP
authors.lastname: Cheon
keywords: Adipogenesis.; Caffeine; Cellulite; Diphlorethohydroxycarmalol; Extracellular fibril; Extracellular matrix; Mouse subcutaneous adipose derived stem cell