title: [Fungal infectivities of implanted catheters due to Candida sp. Biofilms formation and resistance].
abstract: BACKGROUND
  Candidemia are the most common fungal infections in hospitals. However, the catheters are subject to be altered by Candida biofilms which increase the risk of invasive nosocomial infections due to the high resistance to antifungal agents. Therefore, the minimum inhibitory concentrations of planktonic (MIC) and sessile cells (CIMS) were evaluated.
  
  
  METHODS
  To review the in vivo biofilms structures of Candida sp. formed on the inner and/or external surfaces of collected catheters, we used scanning electron microscopy (SEM). The level of biofilm resistance was assessed against two conventional antifungal agents: amphotericin B (AmB), which belongs to the class of polyenes, and fluconazole (FLZ) which is an azole.
  
  
  RESULTS
  The SEM observation of biofilms of Candida sp. reveals complex structures. Compared to MICs, the calculation of CIMS showed an increase of 32 times with AmB and of 128 times with FLZ.
  
  
  CONCLUSIONS
  Catheters offer an ideal surface to Candida sp. to form biofilms. This complex structure induces the increase of the resistance of sessile cells against two antifungal agents, AmB and FLZ.
authors.affiliation: Laboratoire antibiotique antifongique, physico-chimie, synthèse et activité biologique, université de Tlemcen, BP 119, 13000 Imama, Tlemcen, Algérie; University center of Naâma, BP 66, 45000 Naâma, Algérie. Electronic address: seddiki.med@gmail.com.
authors.forename: S M L
authors.initials: SM
authors.lastname: Seddiki
authors.affiliation: Laboratoire antibiotique antifongique, physico-chimie, synthèse et activité biologique, université de Tlemcen, BP 119, 13000 Imama, Tlemcen, Algérie.
authors.forename: Z
authors.initials: Z
authors.lastname: Boucherit-Otmani
authors.affiliation: University center of Naâma, BP 66, 45000 Naâma, Algérie.
authors.forename: K
authors.initials: K
authors.lastname: Boucherit
authors.affiliation: Dennis Kunkel Microscopy Inc, P.O. Box 2008, Kailua, HI, 96734, États-Unis.
authors.forename: D
authors.initials: D
authors.lastname: Kunkel
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development; D002175:Candida / Q000145:classification / Q000187:drug effects / Q000254:growth & development / Q000648:ultrastructure; D055499:Catheter-Related Infections / Q000382:microbiology*; D002408:Catheters, Indwelling / Q000009:adverse effects / Q000382:microbiology*; D003428:Cross Infection / Q000382:microbiology; D025141:Drug Resistance, Fungal; D015725:Fluconazole / Q000494:pharmacology; D006801:Humans; D008826:Microbial Sensitivity Tests
chemical_list: D000935:Antifungal Agents; D000666:Amphotericin B; D015725:Fluconazole
keywords: Biofilms; CMI; Candida sp.; Catheters; Cathéters; MIC; Microscopie électronique à balayage; Resistance; Résistance; Scanning electron microscopy