title: Electrochemical Imaging of Dopamine Release from Three-Dimensional-Cultured PC12 Cells Using Large-Scale Integration-Based Amperometric Sensors.
abstract: In the present study, we used a large-scale integration (LSI)-based amperometric sensor array system, designated Bio-LSI, to image dopamine release from three-dimensional (3D)-cultured PC12 cells (PC12 spheroids). The Bio-LSI device consists of 400 sensor electrodes with a pitch of 250 μm for rapid electrochemical imaging of large areas. PC12 spheroids were stimulated with K(+) to release dopamine. Poststimulation dopamine release from the PC12 spheroids was electrochemically imaged using the Bio-LSI device. Bio-LSI clearly showed the effects of the dopaminergic drugs l-3,4-dihydroxyphenylalanine (L-DOPA) and reserpine on K(+)-stimulated dopamine release from PC12 spheroids. Our results demonstrate that dopamine release from PC12 spheroids can be monitored using the device, suggesting that the Bio-LSI is a promising tool for use in evaluating 3D-cultured dopaminergic cells and the effects of dopaminergic drugs. To the best of our knowledge, this report is the first to describe electrochemical imaging of dopamine release by PC12 spheroids using LSI-based amperometric sensors.
authors.affiliation: †Graduate School of Environmental Studies, Tohoku University, 6-6-11-604 Aramaki-aza Aoba, Aoba-ku, Sendai 980-8579, Japan.
authors.forename: Hiroya
authors.initials: H
authors.lastname: Abe
authors.affiliation: †Graduate School of Environmental Studies, Tohoku University, 6-6-11-604 Aramaki-aza Aoba, Aoba-ku, Sendai 980-8579, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Ino
authors.affiliation: ‡Nanobioengineering/Nanobioelectronics Laboratory, Department of Biomedical Engineering, Florida International University, 10555 West Flagler Street, Miami, Florida 33174, United States.|§WPI-Advanced Institute for Materials Research, Tohoku University, 2-1-1 Katahira, Aoba, Sendai 980-8577, Japan.
authors.forename: Chen-Zhong
authors.initials: CZ
authors.lastname: Li
authors.affiliation: †Graduate School of Environmental Studies, Tohoku University, 6-6-11-604 Aramaki-aza Aoba, Aoba-ku, Sendai 980-8579, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kanno
authors.affiliation: †Graduate School of Environmental Studies, Tohoku University, 6-6-11-604 Aramaki-aza Aoba, Aoba-ku, Sendai 980-8579, Japan.
authors.forename: Kumi Y
authors.initials: KY
authors.lastname: Inoue
authors.affiliation: ∥Japan Aviation Electronics Industry, Ltd. 1-1, Musashino 3-chome, Akishima-shi, Tokyo 196-8555, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Suda
authors.affiliation: ∥Japan Aviation Electronics Industry, Ltd. 1-1, Musashino 3-chome, Akishima-shi, Tokyo 196-8555, Japan.
authors.forename: Ryota
authors.initials: R
authors.lastname: Kunikata
authors.affiliation: ⊥Micro System Integration Center, Tohoku University, 519-1176 Aramaki-aza Aoba, Aoba-ku, Sendai 980-0845, Japan.
authors.forename: Masahki
authors.initials: M
authors.lastname: Matsudaira
authors.affiliation: †Graduate School of Environmental Studies, Tohoku University, 6-6-11-604 Aramaki-aza Aoba, Aoba-ku, Sendai 980-8579, Japan.|§WPI-Advanced Institute for Materials Research, Tohoku University, 2-1-1 Katahira, Aoba, Sendai 980-8577, Japan.|#PRESTO, JST, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan.
authors.forename: Yasufumi
authors.initials: Y
authors.lastname: Takahashi
authors.affiliation: †Graduate School of Environmental Studies, Tohoku University, 6-6-11-604 Aramaki-aza Aoba, Aoba-ku, Sendai 980-8579, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Shiku
authors.affiliation: †Graduate School of Environmental Studies, Tohoku University, 6-6-11-604 Aramaki-aza Aoba, Aoba-ku, Sendai 980-8579, Japan.|§WPI-Advanced Institute for Materials Research, Tohoku University, 2-1-1 Katahira, Aoba, Sendai 980-8577, Japan.
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Matsue
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D004298:Dopamine / Q000032:analysis* / Q000378:metabolism*; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D016716:PC12 Cells; D051381:Rats; D018874:Spheroids, Cellular / Q000166:cytology / Q000378:metabolism
chemical_list: D004298:Dopamine