title: Characterization and identification of the proteins bound to two types of polyhydroxyalkanoate granules in Pseudomonas sp. 61-3.
abstract: Pseudomonas sp. 61-3 accumulates two types of polyhydroxyalkanoates (PHAs), poly(3-hydroxybutyrate) [P(3HB)], and poly(3HB-co-3-hydroxyalkanoates) [P(3HB-co-3HA)], and some proteins associated with their PHA granules have been identified. To date, PhaFPs (GA36) and PhaIPs (GA18) were identified from P(3HB-co-3HA) granules. In this study, the gene encoding GA24 associated with P(3HB) granule was identified as phbPPs. PhbPPs was composed of 192 amino acids with a calculated molecular mass of 20.4 kDa and was assumed to be a phasin. phbFPs gene and unknown ORF were also found on phb locus. PhbFPs was anticipated to be the transcriptional repressor of phbPPs gene. PhbPPs was bound to the P(3HB-co-3HA) granules with 3HB composition of more than 87 mol%, and PhaIPs and PhaFPs were bound to the P(3HB-co-3HA) granules with 3HA (C6-C12) composition of more than 13 mol% in the producing cells, suggesting that localization of these proteins is attributed to the monomer compositions of the copolymers.
authors.affiliation: a Faculty of Environmental and Symbiotic Sciences, Department of Food and Health Sciences , Prefectural University of Kumamoto , Kumamoto , Japan.
authors.forename: Ayaka
authors.initials: A
authors.lastname: Hokamura
authors.forename: Kanako
authors.initials: K
authors.lastname: Fujino
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Isoda
authors.forename: Koji
authors.initials: K
authors.lastname: Arizono
authors.forename: Hideki
authors.initials: H
authors.lastname: Shiratsuchi
authors.forename: Hiromi
authors.initials: H
authors.lastname: Matsusaki
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000737:chemistry / Q000378:metabolism*; D002479:Inclusion Bodies / Q000737:chemistry / Q000378:metabolism; D008970:Molecular Weight; D011091:Polyesters / Q000737:chemistry / Q000378:metabolism*; D054813:Polyhydroxyalkanoates / Q000737:chemistry / Q000378:metabolism*; D011549:Pseudomonas / Q000378:metabolism* / Q000472:pathogenicity; D000094122:Polyhydroxybutyrates
chemical_list: D001426:Bacterial Proteins; D006885:Hydroxybutyrates; D011091:Polyesters; D054813:Polyhydroxyalkanoates; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates
keywords: biodegradable plastics; phasin; polyhydroxyalkanoate (PHA); polyhydroxyalkanoate granule-associated protein