title: [Preparation of characterization of complete antigen of T-2 toxin and deoxynivalenol].
abstract: OBJECTIVE
  To synthesize the complete antigen of T-2 toxin and deoxynivalenol (DON), respectively.
  
  
  METHODS
  T-2 toxin and DON were coupled with carrier protein after derivation with succinic anhydride. Molecular weights of the conjugates and carrier protein were detected by MALDI-TOF. And the performance of complete antigens were further validated by chequerboard titration using commercial immunoassay kits for T-2 toxin and DON.
  
  
  RESULTS
  The molecular weights of T-2, DON conjugates and BSA were 67641.6, 66680.4 and 66297.7, respectively.
  
  
  CONCLUSIONS
  The preparation of complete antigen of T-2 and DON will greatly facilitated antibody production and immunoassay development.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.forename: Jingjing
authors.initials: J
authors.lastname: Lu
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhou
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Zhao
authors.forename: Yongning
authors.initials: Y
authors.lastname: Wu
mesh_terms: D004338:Drug Combinations; D006801:Humans; D008970:Molecular Weight; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013605:T-2 Toxin; D014255:Trichothecenes
chemical_list: D004338:Drug Combinations; D014255:Trichothecenes; D013605:T-2 Toxin; C007262:deoxynivalenol