title: Synergistic effect of HA and BMP-2 mimicking peptide on the bioactivity of HA/PMMA bone cement.
abstract: HA and bone morphogenetic protein-2 (BMP-2) possess superior osteoinductive and osteoconductive activities in the repair of bone defects. In this study, a BMP-2 mimicking oligopeptide (serine-serine-valine-proline-threonine, SSVPT) was introduced to the surface of nano-sized HA as the reinforcement phase of a poly(methyl methacrylate) (PMMA) cement, and the synergistic action of HA and oligopeptide on the bioactivity of HA/PMMA bone cement was investigated. Incorporated with 3-trimethoxysilyl propyl methacrylate (MPS)-modified HA, the compressive strength, flexural strength, and flexural modulus of HA/PMMA complied with the international standardization organization 5833 standard. Adherence measurement demonstrated that the introduction of HA and SSVPT could promote the adhesion and proliferation of human fetal osteoblast (hFOB) on the surface of HA/PMMA cement. The increased alkaline phosphatase (ALP) activity indicated that HA/PMMA could promote osteogenic differentiation of hFOB. All of the results illuminated that the HA/PMMA cement could be used as a bioactive material for regeneration and reconstruction of load-bearing bone.
authors.affiliation: Department of Biomedical Engineering, School of Engineering, Sun Yat-sen University, Guangzhou 510006, China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Department of Biomedical Engineering, School of Engineering, Sun Yat-sen University, Guangzhou 510006, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Kang
authors.affiliation: Department of Biomedical Engineering, School of Engineering, Sun Yat-sen University, Guangzhou 510006, China.
authors.forename: Minyu
authors.initials: M
authors.lastname: Rao
authors.affiliation: Department of Biomedical Engineering, School of Engineering, Sun Yat-sen University, Guangzhou 510006, China.
authors.forename: Kejia
authors.initials: K
authors.lastname: Li
authors.affiliation: Department of Biomedical Engineering, School of Engineering, Sun Yat-sen University, Guangzhou 510006, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Biomedical Engineering, School of Engineering, Sun Yat-sen University, Guangzhou 510006, China. Electronic address: quancy2010@163.com.
authors.forename: Changyun
authors.initials: C
authors.lastname: Quan
authors.affiliation: Department of Biomedical Engineering, School of Engineering, Sun Yat-sen University, Guangzhou 510006, China. Electronic address: zhchao9@mail.sysu.edu.cn.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Biomedical Engineering, School of Engineering, Sun Yat-sen University, Guangzhou 510006, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Jiang
authors.affiliation: Department of Orthopedics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China.
authors.forename: Huiyong
authors.initials: H
authors.lastname: Shen
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000595:Amino Acid Sequence; D040761:Biomimetic Materials / Q000737:chemistry; D001843:Bone Cements / Q000737:chemistry* / Q000494:pharmacology; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004357:Drug Synergism; D005333:Fetus; D006801:Humans; D006882:Hydroxyapatites / Q000737:chemistry* / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D009842:Oligopeptides / Q000737:chemistry* / Q000494:pharmacology; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D019904:Polymethyl Methacrylate / Q000737:chemistry* / Q000494:pharmacology; D013499:Surface Properties
chemical_list: D001843:Bone Cements; D055396:Bone Morphogenetic Protein 2; D006882:Hydroxyapatites; D009842:Oligopeptides; D019904:Polymethyl Methacrylate; D000469:Alkaline Phosphatase
keywords: BMP-2 mimicking peptide; Bioactivity; Nano-hydroxyapatite; Poly(methyl methacrylate); Synergistic action