title: Development of fluorescent probes based on protection-deprotection of the key functional groups for biological imaging.
abstract: Recently, the strategy of protection-deprotection of functional groups has been widely employed to design fluorescent probes, as the protection-deprotection of functional groups often induces a marked change in electronic properties. Significant advances have been made in the development of analyte-responsive fluorescent probes based on the protection-deprotection strategy. In this tutorial review, we highlight the representative examples of small-molecule based fluorescent probes for bioimaging, which are operated via the protection-deprotection of key functional groups such as aldehyde, hydroxyl, and amino functional groups reported from 2010 to 2014. The discussion includes the general protection-deprotection methods for aldehyde, hydroxyl, or amino groups, as well as the design strategies, sensing mechanisms, and deprotection modes of the representative fluorescent imaging probes applied to bio-imaging.
authors.affiliation: Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Biological Science and Technology, University of Jinan, Jinan, Shandong 250022, P. R. China. weiyinglin2013@163.com.
authors.forename: Yonghe
authors.initials: Y
authors.lastname: Tang
authors.forename: Dayoung
authors.initials: D
authors.lastname: Lee
authors.forename: Jiaoliang
authors.initials: J
authors.lastname: Wang
authors.forename: Guanhan
authors.initials: G
authors.lastname: Li
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yu
authors.forename: Weiying
authors.initials: W
authors.lastname: Lin
authors.forename: Juyoung
authors.initials: J
authors.lastname: Yoon
mesh_terms: D000818:Animals; D002460:Cell Line; D005456:Fluorescent Dyes; D006801:Humans; D051379:Mice; D057054:Molecular Imaging
chemical_list: D005456:Fluorescent Dyes