title: Isolation and Characterization of L-Tryptophan Ammonia Lyase from Rubrivivax benzoatilyticus Strain JA2.
abstract: Ammonia lyase belongs to the family of enzymes that catalyzes the deamination of amino acids. Depending on the relative activity towards the substrates, L-tryptophan ammonia lyase converts L-tryptophan to indole 3-acrylic acid and ammonia. Here, we isolated, purified, and characterized an L-tryptophan ammonia lyase from phototrophic purple non-sulfur bacterium Rubrivivax benzoatilyticus JA2. The isolated L-tryptophan ammonia lyase found to catalyze the reaction of L-tryptophan to produce indole 3-acrylic acid and NH3. The enzyme is a heterotetramer and has the highest affinity to L-tryptophan. The optimum pH and temperature for the enzymatic action were 7.5 and 35°C, respectively and the Km and Vmax were 40.4 ± 23.1 nM and 0.964±0.2046 s(-1), respectively. These results suggest that the isolated enzyme is highly bioactive and could be a new class. Further molecular analyses are required to confirm the novelty of the enzyme.
authors.affiliation: Department of Genomic Sciences, School of Biological Sciences, Central University of Kerala, P.O. Central University, Kasaragod- 671314, India. RNKumavath@gmail.com.
authors.forename: Ranjith N
authors.initials: RN
authors.lastname: Kumavath
authors.forename: Ch V
authors.initials: ChV
authors.lastname: Ramana
authors.forename: Ch
authors.initials: Ch
authors.lastname: Sasikala
authors.forename: Debmalya
authors.initials: D
authors.lastname: Barh
authors.forename: Alan Prem
authors.initials: AP
authors.lastname: Kumar
authors.forename: Vasco
authors.initials: V
authors.lastname: Azevedo
mesh_terms: D000595:Amino Acid Sequence; D000642:Ammonia-Lyases / Q000737:chemistry / Q000302:isolation & purification*; D020562:Betaproteobacteria / Q000201:enzymology*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014364:Tryptophan / Q000378:metabolism*
chemical_list: D014364:Tryptophan; D000642:Ammonia-Lyases