title: Development of low-fluorescence thick photoresist for high-aspect-ratio microstructure in bio-application.
abstract: In this study, we propose and evaluate a novel low-auto-fluorescence photoresist (SJI photoresist) for bio-application, e.g., in gene analysis and cell assay. The spin-coated SJI photoresist has a wide thickness range of ten to several hundred micrometers, and photoresist microstructures with an aspect ratio of over 7 and micropatterns of less than 2 μm are successfully fabricated. The emission spectrum intensity of the SJI photoresist is found to be over 80% less than that of the widely used SU-8 photoresist. To evaluate the validity of using the proposed photoresist in bio-application for fluorescence observation, we demonstrate a chromosome extension device composed of the SJI photoresist. The normalized contrast ratio of the SJI photoresist exhibits a 50% improvement over that of the SU-8 photoresist; thus, the SJI photoresist is a versatile tool for bio-application.
authors.affiliation: Department of Intelligent Mechanical Systems Engineering, Kagawa University , 2217-20 Hayashi-cho, Takamatsu, Kagawa 761-0396, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Tamai
authors.affiliation: Central Research Center, Daicel Corporation , 1239 Shinzaike, Aboshi-ku, Himeji, Hyogo 671-1283, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Maruo
authors.affiliation: Department of Intelligent Mechanical Systems Engineering, Kagawa University , 2217-20 Hayashi-cho, Takamatsu, Kagawa 761-0396, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ueno
authors.affiliation: Department of Intelligent Mechanical Systems Engineering, Kagawa University , 2217-20 Hayashi-cho, Takamatsu, Kagawa 761-0396, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Terao
authors.affiliation: Department of Microengineering, Kyoto University , Kyoto daigaku Katsura, Nishikyo-ku Kyoto 615-8540, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kotera
authors.affiliation: Department of Intelligent Mechanical Systems Engineering, Kagawa University , 2217-20 Hayashi-cho, Takamatsu, Kagawa 761-0396, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Suzuki