title: Effect of chum salmon egg lectin on tight junctions in Caco-2 cell monolayers.
abstract: The effect of a chum salmon egg lectin (CSL3) on tight junction (TJ) of Caco-2 cell monolayers was investigated. The lectin opened TJ as indicated by the decrease of the transepithelial electrical resistance (TER) value and the increase of the permeation of lucifer yellow, which is transported via the TJ-mediated paracellular pathway. The effects of CSL3 were inhibited by the addition of 10 mM L-rhamnose or D-galactose which were specific sugars for CSL3. The lectin increased the intracellular Ca2+ of Caco-2 cell monolayers, that could be inhibited by the addition of L-rhamnose. The fluorescence immunostaining of β-actin in Caco-2 cell monolayers revealed that the cytoskeleton was changed by the CSL3 treatment, suggesting that CSL3 depolymerized β-actin to cause reversible TJ structural and functional disruption. Although Japanese jack bean lectin and wheat germ lectin showed similar effects in the decrease of the TER values and the increase of the intracellular Ca2+, they could not be inhibited by the same concentrations of simple sugars, such as D-glucose and N-acetyl-D-glucosamine.
authors.affiliation: Graduate School of Life Sciences, Tohoku University, Katahira 2-1-1, Aoba-ku, Sendai 980-8577, Japan. timtam200g@gmail.com.
authors.forename: Ryo
authors.initials: R
authors.lastname: Nemoto
authors.affiliation: Graduate School of Life Sciences, Tohoku University, Katahira 2-1-1, Aoba-ku, Sendai 980-8577, Japan. shin0708taro@gmail.com.
authors.forename: Shintaro
authors.initials: S
authors.lastname: Yamamoto
authors.affiliation: Graduate School of Life Sciences, Tohoku University, Katahira 2-1-1, Aoba-ku, Sendai 980-8577, Japan. ogawa@biochem.tohoku.ac.jp.
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Ogawa
authors.affiliation: Department of Biochemistry and Microbiology, Nelson Mandel Metropolitan University, Port Elizabeth 6031, South Africa. ryno.naude@nmmu.ac.za.
authors.forename: Ryno
authors.initials: R
authors.lastname: Naude
authors.affiliation: Graduate School of Life Sciences, Tohoku University, Katahira 2-1-1, Aoba-ku, Sendai 980-8577, Japan. muramoto@biochem.tohoku.ac.jp.
authors.forename: Koji
authors.initials: K
authors.lastname: Muramoto
mesh_terms: D000199:Actins / Q000378:metabolism; D001692:Biological Transport / Q000502:physiology; D018938:Caco-2 Cells; D002118:Calcium / Q000378:metabolism; D045744:Cell Line, Tumor; D003599:Cytoskeleton / Q000378:metabolism; D004527:Egg Proteins / Q000378:metabolism*; D029941:Fish Proteins / Q000378:metabolism*; D005690:Galactose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D012210:Rhamnose / Q000378:metabolism; D019108:Tight Junctions / Q000378:metabolism*
chemical_list: D000199:Actins; D004527:Egg Proteins; D029941:Fish Proteins; D037102:Lectins; D008565:Membrane Proteins; C425164:salmon egg lectin; D005947:Glucose; D012210:Rhamnose; D002118:Calcium; D005690:Galactose