title: Aquimarina agarivorans sp. nov., a genome-sequenced member of the class Flavobacteriia isolated from Gelidium amansii.
abstract: A novel Gram-stain-negative, facultatively anaerobic, rod-shaped, agar-digesting bacterial strain, designated HQM9T, was isolated from the surface of the marine red alga Gelidium amansii collected from the intertidal zone of Weihai, China. Cells of HQM9T were 3.0-4.0 μm long and 0.2-0.3 μm wide and lacked flagella. The new isolate grew optimally at 28-30 °C, at pH 7.0-7.5, and in the presence of 2.5-3.0% NaCl. The predominant cellular fatty acids were iso-C15 : 0 and iso-C17 : 0 3-OH. The sole menaquinone was MK-6. The DNA G+C content was 33 mol%. The major polar lipids were comprised of phosphatidylethanolamine and four unknown polar lipids. Based on the 16S rRNA gene sequence, the closest relative was Aquimarina agarilytica ZC1T with 97.16% sequence similarity, with which strain HQM9T formed a distinct cluster belonging to the genus Aquimarina in a phylogenetic tree. Moreover, average nucleotide identity and estimated DNA-DNA hybridization values between strains HQM9T and ZC1T were 78.7% and 12.50 ± 2.95%, respectively. On the basis of phenotypic, chemotaxonomic and phylogenetic analysis, strain HQM9T represents the type strain of a novel species within the genus Aquimarina in the family Flavobacteriaceae, phylum Bacteroidetes, for which the name Aquimarina agarivorans sp. nov. is proposed. The type strain is HQM9T ( = ATCC BAA-2612T = CICC 10835T).
authors.affiliation: College of Marine Science, Shandong University at Weihai, Weihai 264209, PR China.
authors.forename: Yan-Xia
authors.initials: YX
authors.lastname: Zhou
authors.affiliation: College of Marine Science, Shandong University at Weihai, Weihai 264209, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: College of Marine Science, Shandong University at Weihai, Weihai 264209, PR China.|State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China.
authors.forename: Zong-Jun
authors.initials: ZJ
authors.lastname: Du
authors.affiliation: College of Marine Science, Shandong University at Weihai, Weihai 264209, PR China.|State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China.
authors.forename: Guan-Jun
authors.initials: GJ
authors.lastname: Chen
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D000461:Rhodophyta / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C042455:menaquinone 6