title: Vasorelaxation effect of gastrodin on isolated thoracic aorta rings of rats.
abstract: OBJECTIVE
  To study the effect of gastrodin on isolated thoracic aorta rings of rats and to investigate the potential mechanism.
  
  
  METHODS
  A perfusion model of isolated thoracic aorta rings of rats was applied. The effect of cumulative gastrodin (5, 50, 100,150, 200, and 250 μmol/L) on endothelium-intact aorta rings was investigated. The same procedure was applied to observe the effect of gastrodin on endothelium-intact/denuded aorta rings pre-contracted with 10(-6) mol/L phenylephrine hydrochloride (PE). The aorta rings incubated by 200 mmol/L gastrodin in the Ca(2+)-free (K-H) solution was contracted by using PE. The effect of 200 mmol/L gastrodin on endothelium-denuded aorta rings pre-contracted with 60 mmol/L KCl was also observed.
  
  
  RESULTS
  Compared with the denuded gastrodin group, the intact gastrodin group could significantly relax the PE-contracted aorta rings (P<0.01). In Ca(2+)-free (K-H) solution KHS, the PE-induced contraction rate of aorta rings pre-incubated by gastrodin was 6.5%±0.7%, which was significantly less than the control group (11.8%±0.9%,P<0.01). However, after 3 mmol/L CaCl2 was added, the Ca(2+)-induced contraction in the gastrodin group (51.7%±2.4%) was similar to that in the control group (49.8%±2.8%). The contractile rate of rings in the KCl-contracted gastrodin group (96.3%±0.6%) was not significantly different from that in the control group (96.8%±1.2%).
  
  
  CONCLUSIONS
  Gastrodin has the effect of vasorelaxation on isolated thoracic aorta rings of rats. The mechanism of the vasorelaxation of gastrodin may mainly work through the inhibition of inositol 1, 4, 5-trisphosphosphate receptor on the sarcoplasmic reticulum of the arterial smooth muscle, which leads to the reduction of the Ca(2+) released from the sarcoplasmic reticulum.
authors.affiliation: Department of Physiology, Medical Science College, China Three Gorges University, Yichang, Hubei Province, 443002, China.
authors.forename: Yuan-long
authors.initials: YL
authors.lastname: Xie
authors.affiliation: Department of Physiology, Medical Science College, China Three Gorges University, Yichang, Hubei Province, 443002, China. Zhoumin@ctgu.edu.cn.
authors.forename: Min
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Department of Physiology, Medical Science College, China Three Gorges University, Yichang, Hubei Province, 443002, China.
authors.forename: Hui-hao
authors.initials: HH
authors.lastname: Ma
authors.affiliation: Department of Physiology, Medical Science College, China Three Gorges University, Yichang, Hubei Province, 443002, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Physiology, Medical Science College, China Three Gorges University, Yichang, Hubei Province, 443002, China.
authors.forename: Ju-ju
authors.initials: JJ
authors.lastname: Liu
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects* / Q000502:physiology; D001592:Benzyl Alcohols / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D004730:Endothelium, Vascular / Q000502:physiology; D005260:Female; D005960:Glucosides / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D010656:Phenylephrine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D014664:Vasodilation / Q000187:drug effects*
chemical_list: D001592:Benzyl Alcohols; D005960:Glucosides; D010656:Phenylephrine; C045345:gastrodin; D002118:Calcium
keywords: Chinese medicine; gastrodin; thoracic aorta ring; vasorelaxation