title: Effect of curcumin on p38MAPK expression in DSS-induced murine ulcerative colitis.
abstract: The aim of this study was to determine the therapeutic effect of curcumin on dextran sulfate sodium-induced ulcerative colitis (UC) and to explore the related mechanism. Sixty mice were randomly divided into 6 groups. A group was the normal control group; B group was the model group; C group was the 1.5 mg/kg dexamethasone group based on the B group; and D, E and F groups were 15, 30, and 60 mg/kg curcumin groups, respectively, based on the B group. The mice were killed 7 days after treatment; the expression of TNF-α and MPO in colon tissue was determined with ELISA, and colon p-p38MAPK and p38MAPK mRNA expression was evaluated by immunohistochemistry and RT-PCR, respectively. In the C, D, E, and F groups, TNF-α and MPO levels significantly decreased (P < 0.05), and the expression of p-p38MAPK also significantly decreased (P < 0.01). The expression of p38MAPK mRNA in the C, D, E, and F groups decreased (P < 0.01), and there was a statistically significant difference between the E and F groups (P < 0.01). Curcumin had a therapeutic effect, which probably played a role in UC treatment by inhibiting the p38MAPK signaling pathway, thereby reducing the release of TNF-α.
authors.affiliation: Department of Gastroenterology, Affiliated Hospital of Luzhou Medical College, Luzhou, Sichuan Province, China ChangPingLicn@163.com.
authors.forename: C-P
authors.initials: CP
authors.lastname: Li
authors.affiliation: West China School of Medicine, Sichuan University, Chengdu, Sichuan Province, China.
authors.forename: J-H
authors.initials: JH
authors.lastname: Li
authors.affiliation: Department of Gastroenterology, Affiliated Hospital of Luzhou Medical College, Luzhou, Sichuan Province, China.
authors.forename: S-Y
authors.initials: SY
authors.lastname: He
authors.affiliation: Department of Gastroenterology, People's Hospital of Ya'an City, Sichuan Province, China.
authors.forename: O
authors.initials: O
authors.lastname: Chen
authors.affiliation: Department of Gastroenterology, Affiliated Hospital of Luzhou Medical College, Luzhou, Sichuan Province, China.
authors.forename: L
authors.initials: L
authors.lastname: Shi
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000188:drug therapy* / Q000201:enzymology; D003106:Colon / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D003474:Curcumin / Q000494:pharmacology*; D016264:Dextran Sulfate; D004353:Drug Evaluation, Preclinical; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D016179:Granulocyte Colony-Stimulating Factor / Q000378:metabolism; D007377:Interleukin-3 / Q000378:metabolism; D007413:Intestinal Mucosa / Q000201:enzymology; D020935:MAP Kinase Signaling System; D008807:Mice, Inbred BALB C; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D007377:Interleukin-3; D011993:Recombinant Fusion Proteins; D014409:Tumor Necrosis Factor-alpha; C404145:myelopoietin; D016179:Granulocyte Colony-Stimulating Factor; D016264:Dextran Sulfate; D048051:p38 Mitogen-Activated Protein Kinases; D003474:Curcumin