title: Expression of SATB1 and HER2 in breast cancer and the correlations with clinicopathologic characteristics.
abstract: BACKGROUND
  Special AT-rich sequence binding protein 1 (SATB1) is found acting as a "genome organizer" that functions as a landing platform to regulate tissue-specific gene ex-pression. In breast cancer cell lines it has been proven that SATB1 could upregulate the expression of the HER2. In this paper, the relevance of SATB1 and HER2 expression was assessed in human breast cancer tissues, and their influence on tumor histological grade and patients' survival was explored.
  
  
  METHODS
  Using immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH), 169 patients with breast cancer were assessed for SATB1 expression, HER2 amplification and hormone-receptor (HR) expression. The effects of SATB1 expression on HER2 and HR expression as well as their association with clinicopathologic characteristics were further analyzed by statistical evaluation.
  
  
  RESULTS
  SATB1 expression was correlated with HER2 expression in breast cancer(r = 0.191; p = 0.013). SATB1, HER2 and SATB1/HER2 co-expression was negatively correlated with HR expression (r = -0.228, p = 0.003; r = -0.338, p = 0.000; r = -0.527, p = 0.000, respectively). SATB1 and HER2 single positive and their co-expression were all significantly correlated with higher histological grade (r = 0.239, p = 0.002; r = 0.160, p = 0.038; r = 0.306, p = 0.003, respectively). Multivariate cox regression analyses showed that SATB1 and HER2 were independent risk factors for breast cancer patients, while HR was a protective factor for patients' survival. Comparing to SATB1 or HER2 single positive expression, SATB1/HER2 co-expression tended to have even worse prognosis.
  
  
  CONCLUSIONS
  SATB1 and HER2 performed a synergistic effect in breast cancer. Their expression correlated with poorly differentiated breast cancer and indicated an unfavorable prognosis.
  The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1400555050159723 .
authors.affiliation: Key Laboratory for Reproductive Medicine of Shandong Province, Provincial Hospital Affiliated to Shandong University, Jinan, China. x-liu@163.com.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Liu
authors.affiliation: Central Laboratory, Jinan Central Hospital Affiliated to Shandong University, Jinan, China. cnfu@sina.com.|Shandong Province Key Laboratory for Target Molecule, Jinan, China. cnfu@sina.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Department of Pharmacy, Jinan Central Hospital Affiliated to Shandong University, Jinan, China. 93677920@qq.com.
authors.forename: Chuanwu
authors.initials: C
authors.lastname: Qiao
authors.affiliation: Department of Pathology, Jinan Central Hospital Affiliated to Shandong University, Jinan, China. 10531976@qq.com.
authors.forename: Fei
authors.initials: F
authors.lastname: Qv
authors.affiliation: Medical Research & Laboratory Diagnostic Center, Jinan Central Hospital Affiliated to Shandong University, Jinan, China. wind.1060@163.com.|Current Affiliation: Department of Thoracic Surgery, Cancer Insititute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. wind.1060@163.com.
authors.forename: Jingnan
authors.initials: J
authors.lastname: Wang
authors.affiliation: Medical Research & Laboratory Diagnostic Center, Jinan Central Hospital Affiliated to Shandong University, Jinan, China. 19769594@qq.com.
authors.forename: Butong
authors.initials: B
authors.lastname: Ding
authors.affiliation: Department of Oncology, Jinan Central Hospital Affiliated to Shandong University, Jinan, China. sunyuping@live.cn.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Medical Research & Laboratory Diagnostic Center, Jinan Central Hospital Affiliated to Shandong University, Jinan, China. sdjnwys@hotmail.com.|Shandong Province Key Laboratory for Target Molecule, Jinan, China. sdjnwys@hotmail.com.
authors.forename: Yunshan
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics; D001943:Breast Neoplasms / Q000737:chemistry* / Q000235:genetics / Q000401:mortality / Q000473:pathology; D002454:Cell Differentiation; D016009:Chi-Square Distribution; D005260:Female; D005784:Gene Amplification; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D053208:Kaplan-Meier Estimate; D036961:Matrix Attachment Region Binding Proteins / Q000032:analysis*; D008875:Middle Aged; D015999:Multivariate Analysis; D060787:Neoplasm Grading; D011237:Predictive Value of Tests; D016016:Proportional Hazards Models; D065840:Protective Factors; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis* / Q000235:genetics; D011960:Receptors, Estrogen / Q000032:analysis; D011980:Receptors, Progesterone / Q000032:analysis; D012307:Risk Factors
chemical_list: D014408:Biomarkers, Tumor; D036961:Matrix Attachment Region Binding Proteins; D018719:Erb-b2 Receptor Tyrosine Kinases; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; C076189:SATB1 protein, human; C508053:ERBB2 protein, human