title: Silk fibroin/gelatin-chondroitin sulfate-hyaluronic acid effectively enhances in vitro chondrogenesis of bone marrow mesenchymal stem cells.
abstract: Tissue engineering is becoming promising for cartilage repair due to the limited self-repair capacity of cartilage tissue. We previously fabricated and characterized a three-dimensional silk fibroin/gelatin-chondroitin sulfate-hyaluronic acid (SF-GCH) scaffold and showed that it could promote proliferation of human bone marrow mesenchymal stem cells (BM-MSCs). This study aimed to evaluate its biological performance as a new biomimetic material for chondrogenic induction of BM-MSCs in comparison to an SF scaffold and conventional pellet culture. We found that the SF-GCH scaffold significantly enhanced the proliferation and chondrogenic differentiation of BM-MSCs compared to the SF scaffold and pellet culture in which the production of sulfated glycoaminoglycan was increased in concordance with the up-regulation of chondrogenic-specific gene markers. Our findings indicate the significant role of SF-GCH by providing a supportive structure and the mimetic cartilage environment for chondrogenesis which enables cartilage regeneration. Thus, our fabricated SF-GCH scaffold may serve as a potential biomimetic material for cartilage tissue engineering.
authors.affiliation: Biomedical Sciences, Graduate School, Khon Kaen University, Khon Kaen 40002, Thailand; Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Nopporn
authors.initials: N
authors.lastname: Sawatjui
authors.affiliation: Department of Oral Diagnosis, Faculty of Dentistry, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Teerasak
authors.initials: T
authors.lastname: Damrongrungruang
authors.affiliation: Stem Cell Therapy and Transplantation Research Group, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand; School of Microbiology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
authors.forename: Wilairat
authors.initials: W
authors.lastname: Leeanansaksiri
authors.affiliation: Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Patcharee
authors.initials: P
authors.lastname: Jearanaikoon
authors.affiliation: Department of Pediatrics, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand.
authors.forename: Suradej
authors.initials: S
authors.lastname: Hongeng
authors.affiliation: Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand. Electronic address: temduang@kku.ac.th.
authors.forename: Temduang
authors.initials: T
authors.lastname: Limpaiboon
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D020219:Chondrogenesis / Q000502:physiology; D002809:Chondroitin Sulfates / Q000737:chemistry* / Q000494:pharmacology; D005349:Fibroins / Q000737:chemistry* / Q000494:pharmacology; D005780:Gelatin / Q000737:chemistry* / Q000494:pharmacology; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000494:pharmacology; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D047011:Silk / Q000737:chemistry*; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D047011:Silk; D005780:Gelatin; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates; D005349:Fibroins
keywords: Biomaterials; Biomimetic; Cartilage tissue engineering; Chondrogenesis; Mesenchymal stem cells; Scaffold