title: Electrochemiluminescence immunosensor based on multifunctional luminol-capped AuNPs@Fe3O4 nanocomposite for the detection of mucin-1.
abstract: In this work, a novel and multifunctional nanocomposite of luminol capped gold modified Fe3O4 (Lu-AuNPs@Fe3O4) was utilized as the carrier of secondary antibody (Ab2) to fabricate a sandwiched electrochemiluminescence (ECL) immunosensor for ultrasensitive detection of mucin-1 (MUC1). Herein, the luminol capped gold nanoparticles (Lu-AuNPs) were synthesized with HAuCl4 and luminol by the help of NaBH4 at room temperature, and then Lu-AuNPs were adsorbed on the Fe3O4 magnetic nanoparticles (MNPs) to form the nanocomposite of Lu-AuNPs@Fe3O4 via electrostatic interaction. Fe3O4 MNPs in Lu-AuNPs@Fe3O4 exhibited excellent conductivity and admirable catalytic activity in H2O2 decomposition, which could enhance the ECL efficiency of luminol-H2O2 system. In addition, the substrates of gold coated ZnO nanoparticles (AuNPs@ZnO), providing large specific surface areas for primary antibody (Ab1) capturing, were modified on the electrode. As a result, a wide linear range of 7 orders of magnitude from 10 fg/mL to 10 ng/mL was obtained with an ultralow detection limit of 4.5 fg/mL for MUC1.
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
authors.forename: Jing-Xi
authors.initials: JX
authors.lastname: Wang
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhuo
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China. Electronic address: yuanruo@swu.edu.cn.
authors.forename: Ruo
authors.initials: R
authors.lastname: Yuan
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China. Electronic address: yqchai@swu.edu.cn.
authors.forename: Ya-Qin
authors.initials: YQ
authors.lastname: Chai
mesh_terms: D003217:Conductometry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry; D007118:Immunoassay / Q000295:instrumentation*; D008163:Luminescent Measurements / Q000295:instrumentation*; D008165:Luminol / Q000737:chemistry*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry; D018396:Mucin-1 / Q000276:immunology; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D058185:Magnetite Nanoparticles; D018396:Mucin-1; D008165:Luminol; D006046:Gold
keywords: Electrochemiluminescence; Immunosensor; Luminol; Lu–AuNPs@Fe(3)O(4); MUC1