title: Comparison of double-locus sequence typing (DLST) and multilocus sequence typing (MLST) for the investigation of Pseudomonas aeruginosa populations.
abstract: Reliable molecular typing methods are necessary to investigate the epidemiology of bacterial pathogens. Reference methods such as multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) are costly and time consuming. Here, we compared our newly developed double-locus sequence typing (DLST) method for Pseudomonas aeruginosa to MLST and PFGE on a collection of 281 isolates. DLST was as discriminatory as MLST and was able to recognize "high-risk" epidemic clones. Both methods were highly congruent. Not surprisingly, a higher discriminatory power was observed with PFGE. In conclusion, being a simple method (single-strand sequencing of only 2 loci), DLST is valuable as a first-line typing tool for epidemiological investigations of P. aeruginosa. Coupled to a more discriminant method like PFGE or whole genome sequencing, it might represent an efficient typing strategy to investigate or prevent outbreaks.
authors.affiliation: Infection Control Department, UMR Chrono-environnement (CNRS), University Hospital of Besançon, Besançon, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Cholley
authors.affiliation: Service of Hospital Preventive Medicine, Lausanne University Hospital, Lausanne, Switzerland.
authors.forename: Milos
authors.initials: M
authors.lastname: Stojanov
authors.affiliation: Infection Control Department, UMR Chrono-environnement (CNRS), University Hospital of Besançon, Besançon, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Hocquet
authors.affiliation: Infection Control Department, UMR Chrono-environnement (CNRS), University Hospital of Besançon, Besançon, France.
authors.forename: Michelle
authors.initials: M
authors.lastname: Thouverez
authors.affiliation: Infection Control Department, UMR Chrono-environnement (CNRS), University Hospital of Besançon, Besançon, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Bertrand
authors.affiliation: Service of Hospital Preventive Medicine, Lausanne University Hospital, Lausanne, Switzerland. Electronic address: Dominique.Blanc@chuv.ch.
authors.forename: Dominique S
authors.initials: DS
authors.lastname: Blanc
mesh_terms: D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D006801:Humans; D017720:Molecular Epidemiology / Q000379:methods; D058889:Molecular Typing / Q000379:methods*; D011552:Pseudomonas Infections / Q000453:epidemiology / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000145:classification* / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods
keywords: MLST; Molecular typing; PFGE; Pseudomonas aeruginosa; Pulsed field gel electrophoresis; Sequence typing