title: Triterpenoids and Polysaccharide Fractions of Ganoderma tsugae Exert Different Effects on Antiallergic Activities.
abstract: This study was to investigate antiallergic effects of triterpenoids (Gt-TRE) and polysaccharide (Gt-PS) extracts from Ganoderma tsugae, using mast cell line RBL-2H3, T cell line EL4, primary T cells, and transfected RAW264.7 macrophage cells. The results showed that histamine secreted from activated RBL-2H3 mast cells was significantly suppressed by Gt-TRE but not Gt-PS. Interleukin- (IL-) 4 secreted from activated EL4 cells was significantly suppressed by Gt-TRE but not Gt-PS. Further primary CD4(+) T cells cultures also confirmed that Gt-TRE (5 ~ 50 µg/mL) significantly suppressed Th2 cytokines IL-4 and IL-5 secretions but had no effect on Th1 cytokines IL-2 and interferon (IFN)-γ. Gt-PS did not affect IL-4 and IL-5 secretions until higher doses (400, 500 µg/mL) and significantly suppressed IFNγ secretions but enhanced IL-2 at these high doses. The reporter gene assay indicated that Gt-TRE inhibited but Gt-PS enhanced the transcriptional activity of NF-κB in activated transfected RAW264.7 cells and transfected EL4 cells. IL-4 secreted by this transfected EL-4 cells was also significantly decreased by Gt-TRE but not by Gt-PS, suggesting that these two fractions may exert different effects on NF-κB related cytokines expression. These data suggested that triterpenoids fraction of Ganoderma tsugae might be the main constituents to alleviate allergic asthma.
authors.affiliation: Department of Nutrition and Health Sciences, College of Health Sciences, Chang Jung Christian University, No. 1 Changda Road, Gueiren District, Tainan 71101, Taiwan.
authors.forename: Miaw-Ling
authors.initials: ML
authors.lastname: Chen
authors.affiliation: Department of Human Development and Family Studies, College of Education, National Taiwan Normal University, No. 162 Hepting East Road, Section 1, Taipei 10610, Taiwan.
authors.forename: Chia-Chien
authors.initials: CC
authors.lastname: Hsieh
authors.affiliation: Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, No. 7 Chung San South Road, Taipei 10002, Taiwan.
authors.forename: Bor-Luen
authors.initials: BL
authors.lastname: Chiang
authors.affiliation: Department of Biochemical Science and Technology, College of Life Science, National Taiwan University, No. 1 Roosevelt Road, Section 4, Taipei 10617, Taiwan.
authors.forename: Bi-Fong
authors.initials: BF
authors.lastname: Lin