title: Biological effects of the electrostatic field: red blood cell-related alterations of oxidative processes in blood.
abstract: The aim of this study was to determine activities of pro-/antioxidant enzymes, reactive oxygen species (ROS) content, and oxidative modification of proteins and lipids in red blood cells (RBCs) and blood plasma of rats exposed to electrostatic field (200 kV/m) during the short (1 h) and the long periods (6 day, 6 h daily). Short-term exposure was characterized by the increase of oxidatively damaged proteins in blood of rats. This was strongly expressed in RBC membranes. After long-term action, RBC content in peripheral blood was higher than in control (P < 0.01) and the attenuation of prooxidant processes was shown.
  
  
  CONCLUSIONS
  External electrostatic field (200 kV/m) alters the balance in pro-/antioxidant processes. We examine oxidative processes in plasma and RBC (hemolysate and membranes). Biological effects of static electric field depend on exposure time. Acute action of electrostatic field (ESF) characterized by activation of the prooxidant processes. Long-term exposure reflected with prevalence of antioxidant activities.
authors.affiliation: Laboratory of Biochemical and Biophysical Investigations, Scientific-Research Center, Yerevan State Medical University after M. Heratsi, Yerevan, 0025, Armenia. hayk@web.am.
authors.forename: Hayk A
authors.initials: HA
authors.lastname: Harutyunyan
authors.affiliation: Laboratory of Biochemical and Biophysical Investigations, Scientific-Research Center, Yerevan State Medical University after M. Heratsi, Yerevan, 0025, Armenia.
authors.forename: Gohar V
authors.initials: GV
authors.lastname: Sahakyan
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D004906:Erythrocyte Count; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D008297:Male; D009097:Multienzyme Complexes / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D010084:Oxidation-Reduction; D050050:Protein Carbonylation; D051381:Rats; D017382:Reactive Oxygen Species / Q000097:blood; D055672:Static Electricity; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D001798:Blood Proteins; D009097:Multienzyme Complexes; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases
keywords: Carbonylation of proteins; Electrostatic field; Oxidative processes; Reactive oxygen species; Red blood cells