title: Effects of hydroxycamptothecin on the expression of matrix metalloproteinase-1 (MMP-1), tissue inhibitor of MMP-1, and type I collagen in rats with pulmonary fibrosis.
abstract: The aim of this study was to investigate the effects of hydroxycamptothecin (HCPT) on the expression of matrix metalloproteinase-1 (MMP-1), tissue inhibitor of MMP-1 (TIMP-1), and type I collagen in the lung tissue of rats with pulmonary fibrosis induced by bleomycin A5. We used hematoxylin eosin staining to observe the degree of pulmonary fibrosis in rats; Masson staining, reverse transcription polymerase chain reaction, and immunohistochemistry were used to observe the expression of collagen, MMP-1, and TIMP-1, and type I collagen. The expression of MMP-1 in the model group decreased significantly, while the expression of TIMP-1 and type I collagen significantly increased. After treatment with HCPT, the degree of pulmonary fibrosis and the expression of TIMP-1 and type I collagen decreased in all treatment groups. However, the expression of MMP-1 increased in a dose-dependent manner. Our results showed that HCPT decreased the pulmonary fibrosis induced by bleomycin A5 in rats, and an increase in MMP-1 expression and decrease in the TIMP-1 and type I collagen expression may be the mechanism that regulates the metabolism of the extracellular matrix.
authors.affiliation: Peking University Shenzhen Hospital, Shenzhen, China.
authors.forename: G-X
authors.initials: GX
authors.lastname: Hu
authors.affiliation: Peking University Shenzhen Hospital, Shenzhen, China.
authors.forename: S-T
authors.initials: ST
authors.lastname: Yao
authors.affiliation: Peking University Shenzhen Hospital, Shenzhen, China.
authors.forename: L-H
authors.initials: LH
authors.lastname: Zeng
authors.affiliation: Peking University Shenzhen Hospital, Shenzhen, China.
authors.forename: Y-Z
authors.initials: YZ
authors.lastname: Peng
authors.affiliation: Peking University Shenzhen Hospital, Shenzhen, China zhengj_zj@163.com.
authors.forename: J
authors.initials: J
authors.lastname: Zheng
mesh_terms: D000818:Animals; D002166:Camptothecin / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D024042:Collagen Type I / Q000378:metabolism*; D005260:Female; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000235:genetics / Q000378:metabolism*; D011658:Pulmonary Fibrosis / Q000188:drug therapy; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics / Q000378:metabolism*
chemical_list: D024042:Collagen Type I; D012333:RNA, Messenger; D019715:Tissue Inhibitor of Metalloproteinase-1; D020781:Matrix Metalloproteinase 1; D002166:Camptothecin