title: miRNA-202 in bone marrow stromal cells affects the growth and adhesion of multiple myeloma cells by regulating B cell-activating factor.
abstract: Bone marrow stromal cells (BMSCs) up-regulate B cell-activating factor (BAFF) in multiple myeloma. Increasing experimental evidence has shown that microRNAs play a causal role in hematology tumorigenesis. In this study, we characterized the role of miR-202 in regulating the expression of BAFF in BMSCs. It was found that expressions of BAFF mRNA and protein were increased in BMSCs treated with miR-202 inhibitor. The growth rate of miR-202 mimics transfection cells was significantly lower than that of non-transfected cells. The expression of Bcl-2 protein was down-regulated, and Bax protein was up-regulated after miR-202 mimics transfection. Over-expression of miR-202 in BMSCs rendered MM cells more sensitive to bortezomib. More significantly, the regulatory effect of miR-202 could inhibit the activation of NF-κB pathway in BMSCs. These results suggest that miR-202 functions as a modulator that can negatively regulate BAFF by inhibiting MM cell survival, growth, and adhesion in the bone marrow microenvironment.
authors.affiliation: Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, #20 Xisi Road, Nantong, 226001, JS, People's Republic of China.
authors.forename: Xianjuan
authors.initials: X
authors.lastname: Shen
authors.affiliation: Nantong University, #9 Seyuan Road, Nantong, 226001, JS, People's Republic of China.
authors.forename: Yuehua
authors.initials: Y
authors.lastname: Guo
authors.affiliation: The First Hospital Affiliated to Soochow University, #20 Shizi Road, Suzhou, 215006, JS, People's Republic of China.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Yu
authors.affiliation: Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, #20 Xisi Road, Nantong, 226001, JS, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Qi
authors.affiliation: Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, #20 Xisi Road, Nantong, 226001, JS, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
authors.affiliation: Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, #20 Xisi Road, Nantong, 226001, JS, People's Republic of China.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Wu
authors.affiliation: Laboratory Medicine Center, Affiliated Hospital of Nantong University, #20 Xisi Road, Nantong, 226001, JS, People's Republic of China.
authors.forename: Hongbing
authors.initials: H
authors.lastname: Ni
authors.affiliation: Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, #20 Xisi Road, Nantong, 226001, JS, People's Republic of China. jsq814@hotmail.com.|Nantong University, #9 Seyuan Road, Nantong, 226001, JS, People's Republic of China. jsq814@hotmail.com.|Laboratory Medicine Center, Affiliated Hospital of Nantong University, #20 Xisi Road, Nantong, 226001, JS, People's Republic of China. jsq814@hotmail.com.
authors.forename: Shaoqing
authors.initials: S
authors.lastname: Ju
mesh_terms: D000368:Aged; D053264:B-Cell Activating Factor / Q000378:metabolism*; D001402:B-Lymphocytes / Q000502:physiology*; D002448:Cell Adhesion; D049109:Cell Proliferation; D016213:Cyclins / Q000032:analysis; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D009101:Multiple Myeloma / Q000473:pathology*; D051028:bcl-2-Associated X Protein / Q000032:analysis
chemical_list: D053264:B-Cell Activating Factor; C494826:BAX protein, human; D016213:Cyclins; C569392:MIRN202 microRNA, human; D035683:MicroRNAs; C505719:TNFSF13B protein, human; D051028:bcl-2-Associated X Protein
keywords: B cell-activating factor (BAFF); Bone marrow stromal cells (BMSCs); Drug resistance; Multiple myeloma (MM); microRNA-202 (miR-202)