title: T-2 toxin inhibits gene expression and activity of key steroidogenesis enzymes in mouse Leydig cells.
abstract: T-2 toxin is one of the mycotoxins, a group of type A trichothecenes produced by several fungal genera including Fusarium species, which may lead to the decrease of the testosterone secretion in the primary Leydig cells derived from the mouse testis. The previous study demonstrated the effects of T-2 toxin through direct decrease of the testosterone biosynthesis in the primary Leydig cells derived from the mouse testis. In this study, we further examined the direct biological effects of T-2 toxin on steroidogenesis production, primarily in Leydig cells of mice. Mature mouse Leydig cells were purified by Percoll gradient centrifugation and the cell purity was determined by 3β-hydroxysteroid dehydrogenase (3β-HSD) staining. To examine T-2 toxin-induced testosterone secretion decrease, we measured the transcription levels of 3 key steroidogenic enzymes and 5 enzyme activities including 3β-HSD-1, P450scc, StAR, CYP17A1, and 17β-HSD in T-2 toxin/human chorionicgonadotropin (hCG) co-treated cells. Our previous study showed that T-2 toxin (10(-7) M, 10(-8) M and 10(-9) M) significantly suppressed hCG (10 ng/ml)-induced testosterone secretion. The studies demonstrated that the suppressive effect is correlated with the decreases in the levels of transcription of 3β-HSD-1, P450scc, and StAR (P<0.05) and also in enzyme activities of 3β-HSD-1, P450scc, StAR, CYP17A1, and 17β-HSD (P<0.05).
authors.affiliation: College of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang, Henan 471003, China.
authors.forename: Jian Ying
authors.initials: JY
authors.lastname: Yang
authors.affiliation: College of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang, Henan 471003, China; College of Food and Bioengineering, Henan University of Science and Technology, Luo Yang, Henan 471023, China. Electronic address: zyf_1001@haust.edu.cn.
authors.forename: Yong Fa
authors.initials: YF
authors.lastname: Zhang
authors.affiliation: College of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang, Henan 471003, China.
authors.forename: Xiang Ping
authors.initials: XP
authors.lastname: Meng
authors.affiliation: College of Animal Science & Technology, Henan University of Science and Technology, Luo Yang, Henan 471003, China.
authors.forename: Yuan Xiao
authors.initials: YX
authors.lastname: Li
authors.affiliation: College of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang, Henan 471003, China.
authors.forename: Kai Wang
authors.initials: KW
authors.lastname: Ma
authors.affiliation: College of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang, Henan 471003, China.
authors.forename: Xue Fei
authors.initials: XF
authors.lastname: Bai
mesh_terms: D015067:17-Hydroxysteroid Dehydrogenases / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000235:genetics / Q000378:metabolism; D006063:Chorionic Gonadotropin; D015870:Gene Expression / Q000187:drug effects; D007985:Leydig Cells / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D013254:Steroid 17-alpha-Hydroxylase / Q000378:metabolism*; D013605:T-2 Toxin / Q000633:toxicity*; D000098682:Steroidogenic Acute Regulatory Protein
chemical_list: D006063:Chorionic Gonadotropin; D010750:Phosphoproteins; D000098682:Steroidogenic Acute Regulatory Protein; D015067:17-Hydroxysteroid Dehydrogenases; C044256:3 (or 17)-beta-hydroxysteroid dehydrogenase; D013254:Steroid 17-alpha-Hydroxylase; D002786:Cholesterol Side-Chain Cleavage Enzyme; D013605:T-2 Toxin
keywords: 17β-HSD; 3β-HSD-1; CYP17A1; Enzyme activity; Mouse Leydig cells; StAR; T-2 toxin; p450scc