title: The microRNA-217 functions as a tumor suppressor and is frequently downregulated in human osteosarcoma.
abstract: OBJECTIVE
  Dysregulation of miRNA is always associated with cancer development and progression. Aberrant expression of miR-217 has been found in some types of cancer. However, its expression and function in osteosarcoma remain unclear. The aim of this study was to explore the effects of miR-217 in osteosarcoma tumorigenesis and development.
  
  
  METHODS
  The expression level of miR-217 was quantified by real-time RT-PCR in human osteosarcoma cell lines and tissues. MTT, flow cytometric, transwell invasion and migration assays, and tumorigenicity in vivo were adopted to observe the effects of miR-217 on MG-63 cell phenotypes.
  
  
  RESULTS
  MiR-217 was significantly downregulated in osteosarcoma cell lines and clinical specimens. Decreased miR-217 expression was significantly associated with large tumor size, positive distant metastasis, and advanced clinical stage. Low miR-217 expression in osteosarcoma was an independent predictor of poor survival. Overexpression of miR-217 can inhibit the proliferation, invasion, migration and promoted apoptosis of MG-63 cells in vitro and in vivo.
  
  
  CONCLUSIONS
  These findings indicate that miR-217 may act as a tumor suppressor in osteosarcoma and would serve as a novel therapeutic agent for miRNA-based therapy.
authors.affiliation: Department of Bone and Soft Tissue Sarcoma The Shandong Tumor Hospital, Jinan Shandong, 250117, China.
authors.forename: Baoyong
authors.initials: B
authors.lastname: Sun
authors.affiliation: Department of Bone and Soft Tissue Sarcoma The Shandong Tumor Hospital, Jinan Shandong, 250117, China. Electronic address: manuyangmingshan@163.com.
authors.forename: Mingshan
authors.initials: M
authors.lastname: Yang
authors.affiliation: Department of Bone and Soft Tissue Sarcoma The Shandong Tumor Hospital, Jinan Shandong, 250117, China.
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Bone and Soft Tissue Sarcoma The Shandong Tumor Hospital, Jinan Shandong, 250117, China.
authors.forename: Fangxin
authors.initials: F
authors.lastname: Wang
mesh_terms: D000818:Animals; D001859:Bone Neoplasms / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D015536:Down-Regulation / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D015999:Multivariate Analysis; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology; D011379:Prognosis; D015854:Up-Regulation / Q000235:genetics; D023041:Xenograft Model Antitumor Assays
chemical_list: C553315:MIRN217 microRNA, human; D035683:MicroRNAs
keywords: Invasion; MiR-217; Osteosarcoma; Prognosis; Proliferation