title: The Risk of the Preparation of Artificial DNAs via an Interrupted Automated Solid-Phase Triester Method.
abstract: In this paper, the risk of the preparation of artificial DNAs via an interrupted automated solid-phase triester procedure is discussed. These results differ completely from the corresponding results obtained by a gradual non-automated synthesis, indicated as solution-phase chemistry. With experimental results focused on model systems under various conditions and molecular-mechanics calculations, it feels as a challenge to offer an explanation for this different behavior. Attention is briefly given to click-linked DNA fragments as a substitute of phosphate linkages.
authors.affiliation: a Professor Emeritus , Tilburg , The Netherlands.
authors.forename: Henk M
authors.initials: HM
authors.lastname: Buck
mesh_terms: D057930:Click Chemistry / Q000379:methods*; D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D004247:DNA
keywords: Modified DNA; click-linked DNA; isomeric backbone modifications; phosphatemethylated (methylphosphotriester) DNA; solid-phase chemistry; solution-phase chemistry