title: The Control of Arabidopsis thaliana Growth by Cell Proliferation and Endoreplication Requires the F-Box Protein FBL17.
abstract: A key step of the cell cycle is the entry into the DNA replication phase that typically commits cells to divide. However, little is known about the molecular mechanisms regulating this transition in plants. Here, we investigated the function of FBL17 (F BOX-LIKE17), an Arabidopsis thaliana F-box protein previously shown to govern the progression through the second mitosis during pollen development. Our work reveals that FBL17 function is not restricted to gametogenesis. FBL17 transcripts accumulate in both proliferating and postmitotic cell types of Arabidopsis plants. Loss of FBL17 function drastically reduces plant growth by altering cell division activity in both shoot and root apical meristems. In fbl17 mutant plants, DNA replication is severely impaired and endoreplication is fully suppressed. At the molecular level, lack of FBL17 increases the stability of the CDK (CYCLIN-DEPENDENT KINASE) inhibitor KIP-RELATED PROTEIN2 known to switch off CDKA;1 kinase activity. Despite the strong inhibition of cell proliferation in fbl17, some cells are still able to enter S phase and eventually to divide, but they exhibit a strong DNA damage response and often missegregate chromosomes. Altogether, these data indicate that the F-box protein FBL17 acts as a master cell cycle regulator during the diploid sporophyte phase of the plant.
authors.affiliation: Institut de Biologie Moléculaire des Plantes, CNRS, Unité Propre de Recherche 2357, Conventionné avec l'Université de Strasbourg, 67084 Strasbourg, France.
authors.forename: Sandra
authors.identifier: http://orcid.org/0000-0002-5666-2885
authors.initials: S
authors.lastname: Noir
authors.affiliation: Institut de Biologie Intégrative des Plantes, Unité de Biochimie et Physiologie Moléculaire des Plantes, 34060 Montpellier, France.
authors.forename: Katia
authors.initials: K
authors.lastname: Marrocco
authors.affiliation: Institut de Biologie Moléculaire des Plantes, CNRS, Unité Propre de Recherche 2357, Conventionné avec l'Université de Strasbourg, 67084 Strasbourg, France.
authors.forename: Kinda
authors.initials: K
authors.lastname: Masoud
authors.affiliation: Institut de Biologie Moléculaire des Plantes, CNRS, Unité Propre de Recherche 2357, Conventionné avec l'Université de Strasbourg, 67084 Strasbourg, France.
authors.forename: Alexis
authors.initials: A
authors.lastname: Thomann
authors.affiliation: Institut de Biologie Moléculaire des Plantes, CNRS, Unité Propre de Recherche 2357, Conventionné avec l'Université de Strasbourg, 67084 Strasbourg, France.
authors.forename: Andi
authors.initials: A
authors.lastname: Gusti
authors.affiliation: Institut de Biologie Moléculaire des Plantes, CNRS, Unité Propre de Recherche 2357, Conventionné avec l'Université de Strasbourg, 67084 Strasbourg, France.
authors.forename: Marta
authors.initials: M
authors.lastname: Bitrian
authors.affiliation: Institut de Biologie Moléculaire des Plantes, CNRS, Unité Propre de Recherche 2357, Conventionné avec l'Université de Strasbourg, 67084 Strasbourg, France.
authors.forename: Arp
authors.initials: A
authors.lastname: Schnittger
authors.affiliation: Institut de Biologie Moléculaire des Plantes, CNRS, Unité Propre de Recherche 2357, Conventionné avec l'Université de Strasbourg, 67084 Strasbourg, France Institut de Biologie Intégrative des Plantes, Unité de Biochimie et Physiologie Moléculaire des Plantes, 34060 Montpellier, France pascal.genschik@ibmp-cnrs.unistra.fr.
authors.forename: Pascal
authors.initials: P
authors.lastname: Genschik
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D002453:Cell Cycle; D002454:Cell Differentiation; D002455:Cell Division; D050756:Cyclin-Dependent Kinase Inhibitor Proteins / Q000235:genetics / Q000378:metabolism; D004261:DNA Replication; D062951:Endoreduplication; D044783:F-Box Proteins / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D018519:Meristem / Q000235:genetics; D008938:Mitosis; D009154:Mutation; D016196:S Phase
chemical_list: C532796:AT3G54650 protein, Arabidopsis; D029681:Arabidopsis Proteins; D050756:Cyclin-Dependent Kinase Inhibitor Proteins; D044783:F-Box Proteins