title: A Convenient RP-HPLC Method for Assay Bioactivities of Angiotensin I-Converting Enzyme Inhibitory Peptides.
abstract: A convenient and accurate reversed-phase high-performance liquid chromatography (RP-HPLC) method for angiotensin I-converting enzyme inhibitory peptides assay was described in this paper. The mobile phase consisted of 70% A (0.05% TFA and 0.05% triethylamine in water, pH = 2.9-3.3) and 30% B (100% acetonitrile) using an Isogradient program. The flow rate was 0.5 mL/min. The absorb wavelength was 226.5 nm; the column temperature was controlled at 25°C. This method for angiotensin I-converting enzyme inhibitory peptides assay was convenient for the Iso-gradient program. The accuracy of the RT-HPLC method was verified by analyzing ACE inhibitory activity of the hydrolysate peptides of silkworm pupae protein, and the results showed that the RT-HPLC method was available for exploring new source of angiotensin I-converting enzyme inhibitory peptides rapidly and veraciously.
authors.affiliation: Institute of Quality Standards for Agriculture Products, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.affiliation: Institute of Quality Standards for Agriculture Products, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institute of Quality Standards for Agriculture Products, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Cai
authors.affiliation: Departmentof Food Science and Nutrition, Zhejiang University, Hangzhou 310058, China.
authors.forename: Qihe
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Departmentof Food Science and Nutrition, Zhejiang University, Hangzhou 310058, China.
authors.forename: Guoqing
authors.initials: G
authors.lastname: He