title: Two-Photon Absorbing Dyes with Minimal Autofluorescence in Tissue Imaging: Application to in Vivo Imaging of Amyloid-β Plaques with a Negligible Background Signal.
abstract: Fluorescence imaging of tissues offer an essential means for studying biological systems. Autofluorescence becomes a serious issue in tissue imaging under excitation at UV-vis wavelengths where biological molecules compete with the fluorophore. To address this critical issue, a novel class of fluorophores that can be excited at ∼900 nm under two-photon excitation conditions and emits in the red wavelength region (≥600 nm) has been disclosed. The new π-extended dipolar dye system shows several advantageous features including minimal autofluorescence in tissue imaging and pronounced solvent-sensitive emission behavior, compared with a widely used two-photon absorbing dye, acedan. As an important application of the new dye system, one of the dyes was developed into a fluorescent probe for amyloid-β plaques, a key biomarker of Alzheimer's disease. The probe enabled in vivo imaging of amyloid-β plaques in a disease-model mouse, with negligible background signal. The new dye system has great potential for the development of other types of two-photon fluorescent probes and tags for imaging of tissues with minimal autofluorescence.
authors.affiliation: †Department of Chemistry, Center for Electro-Photo Behaviors in Advanced Molecular Systems, Pohang University of Science and Technology (POSTECH), 77 Cheongam-Ro, Nam-Gu, Pohang, Gyungbuk 790-784, Republic of Korea.
authors.forename: Dokyoung
authors.initials: D
authors.lastname: Kim
authors.affiliation: †Department of Chemistry, Center for Electro-Photo Behaviors in Advanced Molecular Systems, Pohang University of Science and Technology (POSTECH), 77 Cheongam-Ro, Nam-Gu, Pohang, Gyungbuk 790-784, Republic of Korea.
authors.forename: Hyunsoo
authors.initials: H
authors.lastname: Moon
authors.affiliation: ‡Department of Biochemistry and Biomedical Sciences, College of Medicine, Seoul National University, 103 Daehak-Ro, Jongro-Gu, Seoul 110-799, Republic of Korea.
authors.forename: Sung Hoon
authors.initials: SH
authors.lastname: Baik
authors.affiliation: †Department of Chemistry, Center for Electro-Photo Behaviors in Advanced Molecular Systems, Pohang University of Science and Technology (POSTECH), 77 Cheongam-Ro, Nam-Gu, Pohang, Gyungbuk 790-784, Republic of Korea.
authors.forename: Subhankar
authors.initials: S
authors.lastname: Singha
authors.affiliation: †Department of Chemistry, Center for Electro-Photo Behaviors in Advanced Molecular Systems, Pohang University of Science and Technology (POSTECH), 77 Cheongam-Ro, Nam-Gu, Pohang, Gyungbuk 790-784, Republic of Korea.
authors.forename: Yong Woong
authors.initials: YW
authors.lastname: Jun
authors.affiliation: §Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology (POSTECH), 77 Cheongam-Ro, Nam-Gu, Pohang, Gyungbuk 790-784, Republic of Korea.
authors.forename: Taejun
authors.initials: T
authors.lastname: Wang
authors.affiliation: §Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology (POSTECH), 77 Cheongam-Ro, Nam-Gu, Pohang, Gyungbuk 790-784, Republic of Korea.
authors.forename: Ki Hean
authors.initials: KH
authors.lastname: Kim
authors.affiliation: ⊥Department of Anatomy and Neurobiology, School of Medicine, Biomedical Science Institute, Kyung Hee University, 26 Kyungheedae-Ro, Dongdaemun-Gu, Seoul 130-701, Republic of Korea.
authors.forename: Byung Sun
authors.initials: BS
authors.lastname: Park
authors.affiliation: ⊥Department of Anatomy and Neurobiology, School of Medicine, Biomedical Science Institute, Kyung Hee University, 26 Kyungheedae-Ro, Dongdaemun-Gu, Seoul 130-701, Republic of Korea.
authors.forename: Junyang
authors.initials: J
authors.lastname: Jung
authors.affiliation: ‡Department of Biochemistry and Biomedical Sciences, College of Medicine, Seoul National University, 103 Daehak-Ro, Jongro-Gu, Seoul 110-799, Republic of Korea.
authors.forename: Inhee
authors.initials: I
authors.lastname: Mook-Jung
authors.affiliation: †Department of Chemistry, Center for Electro-Photo Behaviors in Advanced Molecular Systems, Pohang University of Science and Technology (POSTECH), 77 Cheongam-Ro, Nam-Gu, Pohang, Gyungbuk 790-784, Republic of Korea.
authors.forename: Kyo Han
authors.initials: KH
authors.lastname: Ahn
mesh_terms: D000544:Alzheimer Disease / Q000175:diagnosis*; D016229:Amyloid beta-Peptides / Q000032:analysis*; D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival; D004195:Disease Models, Animal; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D015394:Molecular Structure; D061848:Optical Imaging; D017785:Photons
chemical_list: D016229:Amyloid beta-Peptides; D005456:Fluorescent Dyes