title: Biohythane production from marine macroalgae Sargassum sp. coupling dark fermentation and anaerobic digestion.
abstract: Potential biohythane production from Sargassum sp. was evaluated in a two stage process. In the first stage, hydrogen dark fermentation was performed by Caldicellulosiruptor saccharolyticus. Sargassum sp. concentrations (VS) of 2.5, 4.9 and 7.4gL(-1) and initial inoculum concentrations (CDW) of 0.04 and 0.09gL(-1) of C. saccharolyticus were used in substrate/inoculum ratios ranging from 28 to 123. The end products from hydrogen production process were subsequently used for biogas production. The highest hydrogen and methane production yields, 91.3±3.3Lkg(-1) and 541±10Lkg(-1), respectively, were achieved with 2.5gL(-1) of Sargassum sp. (VS) and 0.09gL(-1)of inoculum (CDW). The biogas produced contained 14-20% of hydrogen. Potential energy production from Sargassum sp. in two stage process was estimated in 242GJha(-1)yr(-1). A maximum energy supply of 600EJyr(-1) could be obtained from the ocean potential area for macroalgae production.
authors.affiliation: CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.
authors.forename: José C
authors.initials: JC
authors.lastname: Costa
authors.affiliation: CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.
authors.forename: João V
authors.initials: JV
authors.lastname: Oliveira
authors.affiliation: CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.
authors.forename: Maria A
authors.initials: MA
authors.lastname: Pereira
authors.affiliation: CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.
authors.forename: Maria M
authors.initials: MM
authors.lastname: Alves
authors.affiliation: CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal. Electronic address: angela_abreu@deb.uminho.pt.
authors.forename: Angela A
authors.initials: AA
authors.lastname: Abreu
mesh_terms: D000693:Anaerobiosis / Q000502:physiology; D059001:Aquatic Organisms / Q000378:metabolism; D061249:Batch Cell Culture Techniques / Q000379:methods; D056804:Biofuels / Q000382:microbiology*; D019149:Bioreactors / Q000382:microbiology; D049109:Cell Proliferation / Q000502:physiology; D005285:Fermentation; D006859:Hydrogen / Q000302:isolation & purification / Q000378:metabolism*; D008027:Light; D008697:Methane / Q000302:isolation & purification / Q000378:metabolism*; D044647:Sargassum / Q000145:classification / Q000502:physiology*; D012623:Seawater / Q000382:microbiology*; D013045:Species Specificity; D014871:Water Microbiology
chemical_list: D056804:Biofuels; D006859:Hydrogen; D008697:Methane
keywords: Biohydrogen; Biohythane; Caldicellulosiruptor saccharolyticus; Marine biomass; Sargassum sp.