title: Mathematical model of flagella gene expression dynamics in Salmonella enterica serovar typhimurium.
abstract: Flagellar assembly in Salmonella is controlled by an intricate genetic and biochemical network. This network comprises of a number of inter-connected feedback loops, which control the assembly process dynamically. Critical among these are the FliA-FlgM feedback, FliZ-mediated positive feedback, and FliT-mediated negative feedback. In this work, we develop a mathematical model to track the dynamics of flagellar gene expression in Salmonella. Analysis of our model demonstrates that the network is wired to not only control the transition of the cell from a non-flagellated to a flagellated state, but to also control dynamics of gene expression during cell division. Further, we predict that FliZ encoded in the flagellar regulon acts as a critical secretion-dependent molecular link between flagella and Salmonella Pathogenicity Island 1 gene expression. Sensitivity analysis of the model demonstrates that the flagellar regulatory network architecture is extremely robust to mutations.
authors.affiliation: Department of Chemical Engineering, Indian Institute of Technology Bombay, Powai, Mumbai, 400076 Maharashtra India.
authors.forename: Kirti
authors.initials: K
authors.lastname: Jain
authors.affiliation: Department of Chemical Engineering, Indian Institute of Technology Bombay, Powai, Mumbai, 400076 Maharashtra India.
authors.forename: Amit
authors.initials: A
authors.lastname: Pradhan
authors.affiliation: Department of Chemical Engineering, Indian Institute of Technology Bombay, Powai, Mumbai, 400076 Maharashtra India.
authors.forename: Chaitanya
authors.initials: C
authors.lastname: Mokashi
authors.affiliation: Department of Chemical Engineering, Indian Institute of Technology Bombay, Powai, Mumbai, 400076 Maharashtra India.
authors.forename: Supreet
authors.initials: S
authors.lastname: Saini
keywords: Flagella; Gene regulation; Salmonella; T3SS