title: MicroRNA-429 suppresses cell proliferation, epithelial-mesenchymal transition, and metastasis by direct targeting of BMI1 and E2F3 in renal cell carcinoma.
abstract: BACKGROUND
  MicroRNA-429 (miR-429), a short noncoding RNA belonging to the miR-200 superfamily, plays a crucial role in tumorigenesis and tumor progression. It also acts as a modulator of epithelial-to-mesenchymal transition, a cell development regulating process that affects tumor development and metastasis. The aim of this study was to investigate the potential role of miR-429 in regulating growth and metastasis of renal cell carcinoma.
  
  
  METHODS
  miR-429 expression was stably up-regulated or down-regulated in the renal cell carcinoma ACHN and A498 cell lines, and cell proliferation and metastasis were assessed.
  
  
  RESULTS
  miR-429 overexpression inhibited cell proliferation, colony formation, migration, and invasion. Suppression of endogenous miR-429 promoted cell growth and metastasis. miR-429 was shown to directly target the 3' untranslated regions of B-cell-specific Moloney murine leukemia virus insertion site 1 (BMI1) and E2F transcription factor 3 (E2F3) transcripts, regulating their expression, as well as that of the downstream epithelial-to-mesenchymal transition markers E-cadherin, N-cadherin, vimentin, p14, and p16.
  
  
  CONCLUSIONS
  These results revealed a tumor suppressive role for miR-429 in renal cell carcinoma through directly targeting BMI1 and E2F3.
authors.affiliation: Laboratory of Urology, Guangdong Medical College, Zhanjiang, China.
authors.forename: Mingning
authors.initials: M
authors.lastname: Qiu
authors.affiliation: Laboratory of Urology, Guangdong Medical College, Zhanjiang, China.
authors.forename: Ziji
authors.initials: Z
authors.lastname: Liang
authors.affiliation: Laboratory of Urology, Guangdong Medical College, Zhanjiang, China.
authors.forename: Lieqian
authors.initials: L
authors.lastname: Chen
authors.affiliation: Laboratory of Urology, Guangdong Medical College, Zhanjiang, China.
authors.forename: Guobin
authors.initials: G
authors.lastname: Tan
authors.affiliation: Laboratory of Urology, Guangdong Medical College, Zhanjiang, China.
authors.forename: Kangning
authors.initials: K
authors.lastname: Wang
authors.affiliation: Laboratory of Urology, Guangdong Medical College, Zhanjiang, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.affiliation: Laboratory of Urology, Guangdong Medical College, Zhanjiang, China. Electronic address: jianjunliulab@163.com.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Liu
authors.affiliation: Laboratory of Urology, Guangdong Medical College, Zhanjiang, China. Electronic address: hegechen@163.com.
authors.forename: Hege
authors.initials: H
authors.lastname: Chen
mesh_terms: D015153:Blotting, Western; D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000378:metabolism / Q000556:secondary*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation / Q000502:physiology; D017931:DNA Primers / Q000737:chemistry; D050699:E2F3 Transcription Factor / Q000378:metabolism*; D058750:Epithelial-Mesenchymal Transition; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D035683:MicroRNAs / Q000502:physiology*; D010957:Plasmids; D063150:Polycomb Repressive Complex 1 / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C069077:BMI1 protein, human; D017931:DNA Primers; D050699:E2F3 Transcription Factor; C098319:E2F3 protein, human; C546363:MIRN429 microRNA, human; D035683:MicroRNAs; D063150:Polycomb Repressive Complex 1
keywords: BMI1; E2F3; Renal carcinoma cells; miR-429