title: Screening hybridomas for anabolic androgenic steroids by steroid analog antigen microarray.
abstract: BACKGROUND
  Currently, dozens of anabolic androgenic steroids (AAS) are forbidden in the World Anti-Doping Agency Prohibited List, however, despite extensive investigation, there are still lots of AAS without corresponding monoclonal antibodies.
  
  
  RESULTS
  A steroid analog antigen microarray made up of ten AAS was fabricated to screen the hybridoma and it was found an original unsuccessful clone turned out to be a candidate anti-boldenone antibody, without any cross-reactions with endogenous AAS or 44 different AAS standard reference materials tested.
  
  
  CONCLUSIONS
  Our findings suggested that steroid analog antigen microarray could be a promising tool to screen and characterize new applications of antibodies for structure analogs, and this also exhibits the potential to fast identify effective epitopes of hybridomas in a single assay.
authors.forename: Hongwu
authors.initials: H
authors.lastname: Du
authors.affiliation: 2ImmunoHunt Corporation, Beijing 100071, China.
authors.forename: Guangyu
authors.initials: G
authors.lastname: Chen
authors.forename: Yongzhong
authors.initials: Y
authors.lastname: Bian
authors.forename: Cenzan
authors.initials: C
authors.lastname: Xing
authors.forename: Xue
authors.initials: X
authors.lastname: Ding
authors.forename: Mengliang
authors.initials: M
authors.lastname: Zhu
authors.forename: Yiping
authors.initials: Y
authors.lastname: Xun
authors.forename: Peng
authors.initials: P
authors.lastname: Chen
authors.forename: Yabin
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: 2ImmunoHunt Corporation, Beijing 100071, China.
authors.forename: Shaoxu
authors.initials: S
authors.lastname: Li
mesh_terms: D045930:Anabolic Agents / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D004300:Doping in Sports; D005260:Female; D057166:High-Throughput Screening Assays; D006825:Hybridomas / Q000276:immunology*; D008807:Mice, Inbred BALB C; D040081:Protein Array Analysis; D013256:Steroids / Q000276:immunology*; D015813:Substance Abuse Detection; D013739:Testosterone / Q000031:analogs & derivatives* / Q000276:immunology
chemical_list: D045930:Anabolic Agents; D000911:Antibodies, Monoclonal; D013256:Steroids; D013739:Testosterone; C013915:boldenone