title: Screening biomarkers of bladder cancer using combined miRNA and mRNA microarray analysis.
abstract: Biomarkers, such as microRNAs (miRNAs) may be useful for the diagnosis of bladder cancer. In order to understand the molecular mechanisms underlying bladder cancer, differentially expressed miRNAs (DE-miRNAs) and their target genes in bladder cancer were analyzed. In the present study, miRNA and mRNA expression profiles (GSE40355) were obtained from the Gene Expression Omnibus. These consisted of healthy bladder samples (n=8) and urothelial carcinoma samples (low-grade, n=8 and high-grade, n=8). DE-miRNAs and differentially expressed genes (DEGs) were identified using the limma package and the Benjamin and Hochberg method from the multtest package in R. Target genes of DE-miRNAs were screened. Associations between DEGs were investigated using STRING, and an interaction network was constructed using Cytoscape. Functional and pathway enrichment analyses were performed for DEGs from the interaction network. 87 DE-miRNAs and 2058 DEGs were screened from low-grade bladder cancer samples, and 40 DE-miRNAs and 2477 DEGs were screened from high-grade bladder cancer samples. DE-target genes were significantly associated with the regulation of cell apoptosis. Bladder cancer, non-small cell lung cancer and pancreatic cancer biological pathways were found to be enriched. The results of the present study demonstrated that E2F transcription factor 1, which is targeted by miR-106b, and cyclin-dependent kinase inhibitor 2A (CDKN2A) and V-Erb-B2 avian erythroblastic leukemia viral oncogene homolog-2, which are targeted by miR-125b, participate in the bladder cancer pathway. In conclusion, DE-miRNAs in bladder cancer tissue samples and DE-targeted genes, such as miR-106b and CDKN2A, which were identified in the present study, may provide the basis for targeted therapy for breast cancer and enhance understanding of its pathogenesis.
authors.affiliation: Department of Urology, China‑Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China.
authors.forename: Ning
authors.initials: N
authors.lastname: Jin
authors.affiliation: Department of Urology, China‑Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China.
authors.forename: Xuefei
authors.initials: X
authors.lastname: Jin
authors.affiliation: Department of Urology, China‑Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China.
authors.forename: Xinquan
authors.initials: X
authors.lastname: Gu
authors.affiliation: Department of Urology, China‑Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China.
authors.forename: Wanli
authors.initials: W
authors.lastname: Na
authors.affiliation: Department of Urology, China‑Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China.
authors.forename: Muchun
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Urology, China‑Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhao
mesh_terms: D017209:Apoptosis / Q000235:genetics; D051017:Apoptosis Regulatory Proteins / Q000235:genetics* / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D002277:Carcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D016022:Case-Control Studies; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics / Q000378:metabolism; D050687:E2F1 Transcription Factor / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D053263:Gene Regulatory Networks; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D060787:Neoplasm Grading; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics / Q000378:metabolism; D001743:Urinary Bladder / Q000378:metabolism / Q000473:pathology; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: D051017:Apoptosis Regulatory Proteins; D014408:Biomarkers, Tumor; D019941:Cyclin-Dependent Kinase Inhibitor p16; D050687:E2F1 Transcription Factor; D035683:MicroRNAs; D012333:RNA, Messenger; D018719:Erb-b2 Receptor Tyrosine Kinases; C493753:E2F1 protein, human; C542541:MIRN106 microRNA, human; C531888:MIRN125 microRNA, human; C508053:ERBB2 protein, human