title: Amifostine Increases FAS and Caspase-3 Expression in Colonic Tissue of Irradiated Mice.
abstract: OBJECTIVE
  The aim of this study was to evaluate the expression of FASL, FAS and FADD and caspase-3 in oesophagus, stomach and colonic tissues of mice irradiated in vivo by immunohistochemistry.
  
  
  METHODS
  A total of 48 adult male C57BL mice were distributed into four groups: Ami(-)/Rad(-): Mice received 0.5 ml of 0.9% physiological saline solution (PPS) intraperitioneally (i.p.); Ami(+)/Rad(-): mice received amifostine (400mg/kg i.p.) freshly dissolved in double-distilled water; Ami(-)/Rad(+): mice received 0.5 ml of PSS i.p. 30 min before a single whole-body radiation dose of 7 Gy; Ami(+)/Rad(+): mice received 0.5 ml of an aqueous solution of 400 mg/kg amifostine i.p.30 min prior to irradiation. All groups were assigned into subgroups sacrificed at 0.5 h, 1 h, 2 h and 4 h after irradiation.
  
  
  RESULTS
  In oesophagus and stomach tissues, we did not observe any difference between Ami(-)/ad(-), Ami(+)/Rad(-), Ami(-)/Rad(+) and Ami(+)/Rad(+) groups in the expression of FASL, FAS and FADD. The colonic tissue was the only to exhibit any difference in the expression of FAS and caspase-3 protein in the Ami(-)/Rad(+)group at 1 and 2 h. Amifostine increased FAS and caspase-3 immunoexpression when compared to the control. Immunoexpression for FASL and FADD was not remarkably different in colonic tissue.
  
  
  CONCLUSIONS
  Taken together, our results demonstrate that amifostine increases FAS and caspase-3 expression in colonic tissue of irradiated mice.
authors.affiliation: Department of Pathology, Federal University of São Paulo, SP, Brazil.
authors.forename: Celina T F
authors.initials: CT
authors.lastname: Oshima
authors.affiliation: Department of Biosciences, Federal University of São Paulo, SP, Brazil daribeiro@unifesp.br.
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Ribeiro
authors.affiliation: Department of Pathology, Federal University of São Paulo, SP, Brazil.
authors.forename: Thiago S
authors.initials: TS
authors.lastname: Gomes
authors.affiliation: Department of Clinical and Experimental Oncology, Federal University of São Paulo, SP, Brazil.
authors.forename: Priscila C
authors.initials: PC
authors.lastname: Adios
authors.affiliation: Department of Morphology, Federal University of São Paulo, SP, Brazil.
authors.forename: Mizue I
authors.initials: MI
authors.lastname: Egami
authors.affiliation: Department of Biosciences, Federal University of São Paulo, SP, Brazil.
authors.forename: Helena R C
authors.initials: HR
authors.lastname: Segreto
mesh_terms: D004999:Amifostine / Q000008:administration & dosage*; D000818:Animals; D053148:Caspase 3 / Q000096:biosynthesis*; D003106:Colon / Q000378:metabolism* / Q000473:pathology / Q000528:radiation effects; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects; D006801:Humans; D051379:Mice; D011837:Radiation-Protective Agents / Q000008:administration & dosage; D014916:Whole-Body Irradiation
chemical_list: D011837:Radiation-Protective Agents; D053148:Caspase 3; D004999:Amifostine
keywords: Amifostine; apoptosis; mouse; radiation