title: Enzyme-Responsive Delivery of Multiple Proteins with Spatiotemporal Control.
abstract: Orchestrated biological materials such as enzymes and growth factors regulate the growth of tissues and organs. A chirality-controlled, single-protein technology is devised to tailor the spatiotemporally defined delivery of therapeutic proteins in response to natural enzymes present at wound sites. Sustained delivery of one protein and sequential delivery of two proteins are demonstrated for stroke and skin wound healing.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, 420 Westwood Plaza, Los Angeles, CA, 90095, USA.
authors.forename: Suwei
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, 420 Westwood Plaza, Los Angeles, CA, 90095, USA.
authors.forename: Lina
authors.initials: L
authors.lastname: Nih
authors.affiliation: Department of Neurology, David Geffen School of Medicine, University of California, Los Angeles, 710 Westwood Plaza, Los Angeles, CA, 90095, USA.
authors.forename: S Thomas
authors.initials: ST
authors.lastname: Carmichael
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, 420 Westwood Plaza, Los Angeles, CA, 90095, USA.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, 420 Westwood Plaza, Los Angeles, CA, 90095, USA.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Segura
mesh_terms: D000818:Animals; D020405:Dermis / Q000187:drug effects / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000503:physiopathology; D016503:Drug Delivery Systems / Q000379:methods*; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D004798:Enzymes / Q000378:metabolism*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008807:Mice, Inbred BALB C; D008817:Mice, Mutant Strains; D049329:Nanostructures; D011506:Proteins / Q000008:administration & dosage*; D020574:Proto-Oncogene Proteins c-sis / Q000008:administration & dosage; D012867:Skin / Q000187:drug effects / Q000293:injuries; D020521:Stroke / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D042461:Vascular Endothelial Growth Factor A / Q000008:administration & dosage; D014945:Wound Healing / Q000187:drug effects
chemical_list: D004798:Enzymes; D011506:Proteins; D020574:Proto-Oncogene Proteins c-sis; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A
keywords: chiral peptides; enzyme responsive; protein delivery; sequential release; wound healing