title: 3D high- and super-resolution imaging using single-objective SPIM.
abstract: Single-objective selective-plane illumination microscopy (soSPIM) is achieved with micromirrored cavities combined with a laser beam-steering unit installed on a standard inverted microscope. The illumination and detection are done through the same objective. soSPIM can be used with standard sample preparations and features high background rejection and efficient photon collection, allowing for 3D single-molecule-based super-resolution imaging of whole cells or cell aggregates. Using larger mirrors enabled us to broaden the capabilities of our system to image Drosophila embryos.
authors.affiliation: 1] Interdisciplinary Institute for Neuroscience, University of Bordeaux, Bordeaux, France. [2] Centre National de la Recherche Scientifique, Bordeaux, France. [3] Mechanobiology Institute, National University of Singapore, Singapore.
authors.forename: Remi
authors.initials: R
authors.lastname: Galland
authors.affiliation: Mechanobiology Institute, National University of Singapore, Singapore.
authors.forename: Gianluca
authors.identifier: 0000000193136748
authors.initials: G
authors.lastname: Grenci
authors.affiliation: Mechanobiology Institute, National University of Singapore, Singapore.
authors.forename: Ajay
authors.initials: A
authors.lastname: Aravind
authors.affiliation: 1] Mechanobiology Institute, National University of Singapore, Singapore. [2] Bio Mechanics of Cellular Contacts, Centre National de la Recherche Scientifique, Singapore. [3] Department of Biological Science, National University of Singapore, Singapore.
authors.forename: Virgile
authors.identifier: 0000000339492244
authors.initials: V
authors.lastname: Viasnoff
authors.affiliation: 1] Interdisciplinary Institute for Neuroscience, University of Bordeaux, Bordeaux, France. [2] Centre National de la Recherche Scientifique, Bordeaux, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Studer
authors.affiliation: 1] Interdisciplinary Institute for Neuroscience, University of Bordeaux, Bordeaux, France. [2] Centre National de la Recherche Scientifique, Bordeaux, France.
authors.forename: Jean-Baptiste
authors.initials: JB
authors.lastname: Sibarita
mesh_terms: D000818:Animals; D004330:Drosophila / Q000196:embryology; D021621:Imaging, Three-Dimensional / Q000379:methods*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*