title: Hybridization within Saccharomyces Genus Results in Homoeostasis and Phenotypic Novelty in Winemaking Conditions.
abstract: Despite its biotechnological interest, hybridization, which can result in hybrid vigor, has not commonly been studied or exploited in the yeast genus. From a diallel design including 55 intra- and interspecific hybrids between Saccharomyces cerevisiae and S. uvarum grown at two temperatures in enological conditions, we analyzed as many as 35 fermentation traits with original statistical and modeling tools. We first showed that, depending on the types of trait--kinetics parameters, life-history traits, enological parameters and aromas -, the sources of variation (strain, temperature and strain * temperature effects) differed in a large extent. Then we compared globally three groups of hybrids and their parents at two growth temperatures: intraspecific hybrids S. cerevisiae * S. cerevisiae, intraspecific hybrids S. uvarum * S. uvarum and interspecific hybrids S. cerevisiae * S. uvarum. We found that hybridization could generate multi-trait phenotypes with improved oenological performances and better homeostasis with respect to temperature. These results could explain why interspecific hybridization is so common in natural and domesticated yeast, and open the way to applications for wine-making.
authors.affiliation: INRA, UMR 0320 / UMR 8120 Génétique Végétale, Gif-sur-Yvette, France.
authors.forename: Telma
authors.initials: T
authors.lastname: da Silva
authors.affiliation: ENSCBP-Bordeaux INP, Pessac, France; Université de Bordeaux, ISVV, EA 4577, Unité de recherche Œnologie, Villenave d'Ornon, France.
authors.forename: Warren
authors.initials: W
authors.lastname: Albertin
authors.affiliation: Université Paris-Sud, UMR 0320 / UMR 8120 Génétique Végétale, Gif-sur-Yvette, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Dillmann
authors.affiliation: Université de Bordeaux, ISVV, EA 4577, Unité de recherche Œnologie, Villenave d'Ornon, France.
authors.forename: Marina
authors.initials: M
authors.lastname: Bely
authors.affiliation: SARCO, Bordeaux, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: la Guerche
authors.affiliation: CMAP Ecole Polytechnique, Palaiseau, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Giraud
authors.affiliation: INRA, MIA, Jouy-en-Josas, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Huet
authors.affiliation: Université Paris-Sud, UMR 0320 / UMR 8120 Génétique Végétale, Gif-sur-Yvette, France.
authors.forename: Delphine
authors.initials: D
authors.lastname: Sicard
authors.affiliation: Université de Bordeaux, ISVV, EA 4577, Unité de recherche Œnologie, Villenave d'Ornon, France; Bordeaux Sciences Agro, Gradignan, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Masneuf-Pomarede
authors.affiliation: Université Paris-Sud, UMR 0320 / UMR 8120 Génétique Végétale, Gif-sur-Yvette, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: de Vienne
authors.affiliation: Université de Bordeaux, ISVV, EA 4577, Unité de recherche Œnologie, Villenave d'Ornon, France; Biolaffort, Bordeaux, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Marullo
mesh_terms: D005285:Fermentation / Q000235:genetics*; D006706:Homeostasis; D006824:Hybridization, Genetic; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism