title: Cationic dendronization of amylose via click chemistry for complexation and transfection of plasmid DNA.
abstract: For the development of effective and safe gene carrier based on starch, the amylose from potato starch was azidized by reacting with 3-azidopropylamine in the presence of N, N'-carbonyldiimidazole and then conjugated with propargyl focal point poly(amidoamine) (PAMAM) dendrons by a Cu(I)-catalyzed azide-alkyne cycloaddition. Such a cationic dendronization was verified by Fourier transform infrared spectroscopy and proton nuclear magnetic resonance analyses. For the resultant amylose conjugates with various contents and generations of PAMAM dendron, their buffering capacity, binding ability with plasmid DNA and in vitro cytotoxicity were investigated. These amylose conjugates were found to exhibit good buffering capacity and biocompatibility. In particular, they could condense effectively plasmid DNA into the nanocomplexes, as confirmed by agarose gel electrophoresis, zeta potential, and particle size analyses as well as transmission electron microscopy observation. For their nanocomplexes with plasmid DNA, the in vitro transfection properties in human embryonic kidney 293T cells were studied by fluorescence microscopy and flow cytometry. It was found that the transfection efficiency could be optimized by the dendronization extent of amylose and the complexation extent of dendronized amylose with plasmid DNA.
authors.affiliation: PCFM Lab and GDHPPC Lab, Institute of Polymer Science, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Kaijin
authors.initials: K
authors.lastname: Mai
authors.affiliation: PCFM Lab and GDHPPC Lab, Institute of Polymer Science, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China; Guangdong Medical College, Dongguan 523808, China.
authors.forename: Jiantao
authors.initials: J
authors.lastname: Lin
authors.affiliation: Second Affiliated Hospital, Sun Yat-sen University, Guangzhou 510102, China.
authors.forename: Baoxiong
authors.initials: B
authors.lastname: Zhuang
authors.affiliation: PCFM Lab and GDHPPC Lab, Institute of Polymer Science, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Li
authors.affiliation: PCFM Lab and GDHPPC Lab, Institute of Polymer Science, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China. Electronic address: ceszhlm@mail.sysu.edu.cn.
authors.forename: Li-Ming
authors.initials: LM
authors.lastname: Zhang
mesh_terms: D000688:Amylose / Q000737:chemistry*; D001386:Azides / Q000737:chemistry; D057930:Click Chemistry; D061565:Cycloaddition Reaction; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D050091:Dendrimers / Q000737:chemistry*; D015870:Gene Expression; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D007093:Imidazoles / Q000737:chemistry; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism*; D011437:Propylamines / Q000737:chemistry; D013213:Starch / Q000737:chemistry
chemical_list: D001386:Azides; D050091:Dendrimers; D007093:Imidazoles; C104700:PAMAM Starburst; D011437:Propylamines; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; C006900:N,N-carbonyldiimidazole; D013213:Starch; D000688:Amylose; D004247:DNA
keywords: Amylose; Cationic dendronization; Click conjugation; Gene delivery; Plasmid DNA