title: Expression Analysis of Cathepsin F during Embryogenesis and Early Developmental Stage in Olive Flounder (Paralichthys olivaceus).
abstract: Cathepsins are members of the multigene family of lysosomal cysteine proteinases and have regulated function in several life processes. The potential role of cathepsin F cysteine gene was expected as protease in the yolk processing mechanism during early developmental stage, but expression analysis was unknown after fertilization. The alignment analysis showed that amino acid sequence of cathepsin F from olive flounder liver expressed sequence tag (EST) homologous to cathepsin F of other known cathepsin F sequences with 87-98% identity. In this study, we examined the gene expression analysis of cathepsin F in various tissues at variety age flounder. Tissue distribution of the cathepsin F mRNA has been shown to be ubiquitous and constitutive pattern regardless of age in each group, although derived from cDNA library using liver sample. The mRNA level of cathepsin F more increased as developmental proceed during embryogenesis and early developmental stage, especially increased in the blastula, hatching stage and 3 days post hatching (dph). As a result, it may suggest that the proteolysis of yolk proteins (YPs) has been implicated as a mechanism for nutrient supply during early larval stages in olive flounder.
authors.affiliation: Genetics and Breeding Research Center, NFRDI, Geoje 656-842, Republic of Korea.
authors.forename: Jang-Wook
authors.initials: JW
authors.lastname: Lee
authors.affiliation: Genetics and Breeding Research Center, NFRDI, Geoje 656-842, Republic of Korea.
authors.forename: Young Mee
authors.initials: YM
authors.lastname: Lee
authors.affiliation: Genetics and Breeding Research Center, NFRDI, Geoje 656-842, Republic of Korea.
authors.forename: Hyun
authors.initials: H
authors.lastname: Yang
authors.affiliation: Genetics and Breeding Research Center, NFRDI, Geoje 656-842, Republic of Korea.
authors.forename: Jae Koo
authors.initials: JK
authors.lastname: Noh
authors.affiliation: Genetics and Breeding Research Center, NFRDI, Geoje 656-842, Republic of Korea.
authors.forename: Hyun Chul
authors.initials: HC
authors.lastname: Kim
authors.affiliation: Genetics and Breeding Research Center, NFRDI, Geoje 656-842, Republic of Korea.
authors.forename: Choul-Ji
authors.initials: CJ
authors.lastname: Park
authors.affiliation: Genetics and Breeding Research Center, NFRDI, Geoje 656-842, Republic of Korea.
authors.forename: Jong-Won
authors.initials: JW
authors.lastname: Park
authors.affiliation: Genetics and Breeding Research Center, NFRDI, Geoje 656-842, Republic of Korea.
authors.forename: In Joon
authors.initials: IJ
authors.lastname: Hwang
authors.affiliation: Genetics and Breeding Research Center, NFRDI, Geoje 656-842, Republic of Korea.
authors.forename: Sung Yeon
authors.initials: SY
authors.lastname: Kim
authors.affiliation: Genetics and Breeding Research Center, NFRDI, Geoje 656-842, Republic of Korea.
authors.forename: Jeong-Ho
authors.initials: JH
authors.lastname: Lee
keywords: Cathepsin F; Cysteine proteinase; Developmental stage; Gene expression; Olive flounder; Paralichthys olivaceus; Yolk protein; Yolk syncytial layer (YSL).