title: Characterization and utility of phages bearing peptides with affinity to porcine reproductive and respiratory syndrome virus nsp7 protein.
abstract: High-affinity peptides to porcine reproductive and respiratory syndrome virus (PRRSV) nonstructural protein (nsp) 7 were identified using phage-display technology. Five 12-amino-acid peptide sequences were identified after six rounds of biopanning. A putative CD##WC motif was found in two different consensus peptides borne by phages 4 and 5. The peptides borne by phages 4, 5, and 6 were synthesized for subsequent experiments, according to the results of the binding assays. Immunofluorescence assay revealed that all these peptides recognized nsp7 in PRRSV-infected cells. Furthermore, the peptides demonstrated antiviral activities, with peptides 5 and 6 showing effective inhibition. Early peptide stimulation was associated with strong antiviral activity, and the inhibitory effects of the peptides were dose-dependent at 36 and 48 h post-infection. Peptide 5 was selected to detect the intracellular localization of nsp7 by confocal microscopy. This peptide had a similar effect to anti-nsp7 monoclonal antibody on nsp7. These results suggest that high-affinity peptides to PRRSV nsp7 could mimic the potential of nsp7 antibody as a diagnostic reagent for virus detection. Moreover, the peptides selected in this study represented a potentially effective antiviral candidate to inhibit PRRSV.
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province 510642, People's Republic of China; Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, Guangdong Province 510642, People's Republic of China; MOA Key Laboratory of Animal Vaccine Development, Guangzhou, Guangdong Province 510642, People's Republic of China.
authors.forename: Heng
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province 510642, People's Republic of China; Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, Guangdong Province 510642, People's Republic of China; MOA Key Laboratory of Animal Vaccine Development, Guangzhou, Guangdong Province 510642, People's Republic of China.
authors.forename: Rongchang
authors.initials: R
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province 510642, People's Republic of China; Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, Guangdong Province 510642, People's Republic of China; MOA Key Laboratory of Animal Vaccine Development, Guangzhou, Guangdong Province 510642, People's Republic of China.
authors.forename: Jin
authors.initials: J
authors.lastname: Cui
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province 510642, People's Republic of China; Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, Guangdong Province 510642, People's Republic of China; MOA Key Laboratory of Animal Vaccine Development, Guangzhou, Guangdong Province 510642, People's Republic of China.
authors.forename: Shengchao
authors.initials: S
authors.lastname: Deng
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province 510642, People's Republic of China; Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, Guangdong Province 510642, People's Republic of China; MOA Key Laboratory of Animal Vaccine Development, Guangzhou, Guangdong Province 510642, People's Republic of China.
authors.forename: Jiexiong
authors.initials: J
authors.lastname: Xie
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province 510642, People's Republic of China; Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, Guangdong Province 510642, People's Republic of China; MOA Key Laboratory of Animal Vaccine Development, Guangzhou, Guangdong Province 510642, People's Republic of China.
authors.forename: Zhangyong
authors.initials: Z
authors.lastname: Nin
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province 510642, People's Republic of China; Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, Guangdong Province 510642, People's Republic of China; MOA Key Laboratory of Animal Vaccine Development, Guangzhou, Guangdong Province 510642, People's Republic of China. Electronic address: guihongzh@scau.edu.cn.
authors.forename: Guihong
authors.initials: G
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000302:isolation & purification / Q000378:metabolism*; D003955:Diagnostic Tests, Routine / Q000379:methods; D004358:Drug Therapy / Q000379:methods; D007202:Indicators and Reagents / Q000302:isolation & purification / Q000378:metabolism*; D008403:Mass Screening; D019151:Peptide Library; D010455:Peptides / Q000302:isolation & purification / Q000378:metabolism*; D019318:Porcine Reproductive and Respiratory Syndrome / Q000175:diagnosis / Q000188:drug therapy; D019316:Porcine respiratory and reproductive syndrome virus / Q000187:drug effects*; D011485:Protein Binding; D013552:Swine; D017361:Viral Nonstructural Proteins / Q000378:metabolism*
chemical_list: D000998:Antiviral Agents; D007202:Indicators and Reagents; D019151:Peptide Library; D010455:Peptides; D017361:Viral Nonstructural Proteins
keywords: Antiviral activity; PRRSV; Peptide; Phage display; nsp7 protein