title: Optimization of Extraction, HPLC and Kinetic Studies for Determination of Some Food Tainting Compounds in Different Food Matrices.
abstract: Solid-liquid extraction, ultrasonic-assisted extraction and matrix solid-phase dispersion (MSPD) were optimized and compared in terms of recoveries for the simultaneous extraction of indole (IND) and 2,4-dichlorophenol (DCP) from catfish samples and for the extraction of IND alone from potato samples. Applying high-performance liquid chromatography (HPLC-DAD) procedure using mobile phase of methanol : water (65 : 35) at 280 nm, MSPD was the method of choice for the extraction of IND and DCP from catfish and, also, for IND from potato. The extraction recoveries of MSPD were in the range (97.9-99.7%) and (99.8-100.6%); for IND and DCP, respectively, in catfish samples and (98.4-99.7%) for IND alone in potato samples. Solid-phase extraction (SPE) was chosen the method of choice for the extraction of DCP from fish farms water samples after optimization and comparison with direct sample injection and extraction recoveries were in the range (97.9-100.3%). Kinetics were further studied to follow each of production of IND in catfish during storage at different temperatures and uptake of DCP by tilapia in fish farms water samples using MSPD-HPLC and SPE-HPLC, respectively.
authors.affiliation: Faculty of Pharmacy, Pharmaceutical Analytical Chemistry Department, Ain Shams University, Cairo, Egypt.
authors.forename: A M
authors.initials: AM
authors.lastname: El-Kosasy
authors.affiliation: Faculty of Pharmacy, Pharmaceutical Analytical Chemistry Department, Ain Shams University, Cairo, Egypt.
authors.forename: L A
authors.initials: LA
authors.lastname: Hussin
authors.affiliation: Faculty of Pharmacy, Pharmaceutical Analytical Chemistry Department, Ain Shams University, Cairo, Egypt.
authors.forename: Miriam F
authors.initials: MF
authors.lastname: Ayad
authors.affiliation: Faculty of Pharmacy, Pharmaceutical Analytical Chemistry Department, Ain Shams University, Cairo, Egypt dr.nermine@pharma.asu.edu.eg.
authors.forename: N V
authors.initials: NV
authors.lastname: Fares
mesh_terms: D000818:Animals; D002397:Catfishes; D005591:Chemical Fractionation / Q000379:methods*; D002733:Chlorophenols / Q000032:analysis / Q000302:isolation & purification* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D007211:Indoles / Q000032:analysis / Q000302:isolation & purification* / Q000493:pharmacokinetics; D007700:Kinetics; D057230:Limit of Detection; D016014:Linear Models; D015203:Reproducibility of Results; D011198:Solanum tuberosum / Q000737:chemistry
chemical_list: D002733:Chlorophenols; D007211:Indoles; C030374:indole; C004762:2,4-dichlorophenol