title: Oscheius onirici sp. n. (Nematoda: Rhabditidae): a new entomopathogenic nematode from an Italian cave.
abstract: Oscheius onirici sp. n. (Nematoda: Rhabditidae) was isolated from a karst cave soil of Central Italy. Molecular and morphological analyses were performed. Total DNA was extracted from individual nematodes and the mitochondrial COI, the ITS containing region, the D2-D3 expansion domains of the 28S rRNA gene and the 18S rRNA gene were amplified and sequenced. BLAST search at NCBI by using all molecular markers revealed that this taxon is similar to Oscheius species. Phylogenetic trees of ITS, 28S and 18S rDNA revealed that O. onirici sp. n. belongs to Dolichura-group. Oscheius onirici sp. n. is characterized by small body size and stoma rhabditoid type. Female reproductive system is amphidelphic. Males are rare with peloderan bursa, spicules slender and small, nine pairs of papillae of different lengths, arranged in a 1+1+1/3+3 pattern. Entomopathogenicity bioassay revealed that this nematode is capable of infecting larvae of Galleria mellonella and Tenebrio molitor.
authors.affiliation: Council for Agricultural Research and Economics - Agrobiology and Pedology Research Centre (CRA-ABP), Via Lanciola 12/A, Cascine del Riccio, 50125 Firenze (Italy).; Email: giulia.torrini@isza.it.
authors.forename: Giulia
authors.initials: G
authors.lastname: Torrini
authors.affiliation: Council for Agricultural Research and Economics - Agrobiology and Pedology Research Centre (CRA-ABP), Via Lanciola 12/A, Cascine del Riccio, 50125 Firenze (Italy).; Email: unknown.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Mazza
authors.affiliation: Council for Agricultural Research and Economics - Agrobiology and Pedology Research Centre (CRA-ABP), Via Lanciola 12/A, Cascine del Riccio, 50125 Firenze (Italy).; Email: unknown.
authors.forename: Beatrice
authors.initials: B
authors.lastname: Carletti
authors.affiliation: Council for Agricultural Research and Economics - Agrobiology and Pedology Research Centre (CRA-ABP), Via Lanciola 12/A, Cascine del Riccio, 50125 Firenze (Italy).; Email: unknown.
authors.forename: Claudia
authors.initials: C
authors.lastname: Benvenuti
authors.affiliation: Council for Agricultural Research and Economics - Agrobiology and Pedology Research Centre (CRA-ABP), Via Lanciola 12/A, Cascine del Riccio, 50125 Firenze (Italy).; Email: unknown.
authors.forename: Pio Federico
authors.initials: PF
authors.lastname: Roversi
authors.affiliation: Institute for Sustainable Plant Protection (IPSP)-CNR, via G. Amendola 122/D, 70126 Bari (Italy).; Email: francesca.deluca@ipsp.cnr.it.
authors.forename: Elena
authors.initials: E
authors.lastname: Fanelli
authors.affiliation: Institute for Sustainable Plant Protection (IPSP)-CNR, via G. Amendola 122/D, 70126 Bari (Italy).; Email: francesca.deluca@ipsp.cnr.it.
authors.forename: Francesca
authors.initials: F
authors.lastname: De Luca
authors.affiliation: Institute for Sustainable Plant Protection (IPSP)-CNR, via G. Amendola 122/D, 70126 Bari (Italy).; Email: unknown.
authors.forename: Alberto
authors.initials: A
authors.lastname: Troccoli
authors.affiliation: Institute for Sustainable Plant Protection (IPSP)-CNR, via G. Amendola 122/D, 70126 Bari (Italy). Department of Soil, Plant and Food Sciences, Section of Entomology and Zoology, University of Bari "A. Moro", via G. Amendola, 165/A, 70126 Bari (Italy); Email: unknown.
authors.forename: Eustachio
authors.initials: E
authors.lastname: Tarasco
mesh_terms: D000818:Animals; D059552:Caves; D018509:DNA, Helminth / Q000235:genetics; D021901:DNA, Intergenic / Q000235:genetics; D005260:Female; D007558:Italy; D008297:Male; D009348:Nematoda / Q000145:classification* / Q000235:genetics / Q000648:ultrastructure*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013045:Species Specificity
chemical_list: D018509:DNA, Helminth; D021901:DNA, Intergenic