title: Development of a blocking latex agglutination test for the detection of antibodies to chicken anemia virus.
abstract: A blocking latex agglutination test (b-LAT) developed in this study was evaluated for the detection of antibodies against chicken anemia virus (CAV) in chickens. Polystyrene latex beads were coupled with a neutralizing monoclonal antibody (mAb) to CAV (mAb-beads). When mAb-beads were mixed with antigens prepared from the lysate of MDCC-MSB1 cells infected with CAV, agglutination occurred. A short pre-incubation of CAV antigens with CAV-specific antiserum inhibited the agglutination of mAb-beads. The test results were obtained within 5min. The specificity of b-LAT was evaluated using sera from specific pathogen-free chickens and sera containing antibodies to avian influenza virus, Newcastle disease virus, infectious bursal disease virus, and Marek's disease virus; nonspecific agglutination and cross-reactivity with antibodies to unrelated viruses were not observed. The examination of 94 serum samples collected from commercial breeder chickens of various ages (17-63 weeks) revealed good agreement (93.6%, Kappa value=0.82) between b-LAT and a virus neutralization test, known to be most sensitive and specific in the detection of antibodies to CAV. These results indicate that b-LAT, a simple and rapid test, is a useful and reliable tool in CAV serology.
authors.affiliation: Diagnostic Center for Animal Hygiene and Food Safety, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Inada, Obihiro, Hokkaido 080-8555, Japan; National Institute of Veterinary Research, 86 Truong Chinh, Dong Da, Hanoi, Vietnam.
authors.forename: Dai Quang
authors.initials: DQ
authors.lastname: Trinh
authors.affiliation: Diagnostic Center for Animal Hygiene and Food Safety, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Inada, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Haruko
authors.initials: H
authors.lastname: Ogawa
authors.affiliation: Diagnostic Center for Animal Hygiene and Food Safety, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Inada, Obihiro, Hokkaido 080-8555, Japan; National Institute of Veterinary Research, 86 Truong Chinh, Dong Da, Hanoi, Vietnam.
authors.forename: Vuong Nghia
authors.initials: VN
authors.lastname: Bui
authors.affiliation: National Institute of Veterinary Research, 86 Truong Chinh, Dong Da, Hanoi, Vietnam.
authors.forename: Tham Thi Hong
authors.initials: TT
authors.lastname: Nguyen
authors.affiliation: Diagnostic Center for Animal Hygiene and Food Safety, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Inada, Obihiro, Hokkaido 080-8555, Japan; Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, Thailand.
authors.forename: Dulyatad
authors.initials: D
authors.lastname: Gronsang
authors.affiliation: Diagnostic Center for Animal Hygiene and Food Safety, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Inada, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Tugsbaatar
authors.initials: T
authors.lastname: Baatartsogt
authors.affiliation: Diagnostic Center for Animal Hygiene and Food Safety, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Inada, Obihiro, Hokkaido 080-8555, Japan; College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, Kampala, Uganda.
authors.forename: Mugimba Kahoza
authors.initials: MK
authors.lastname: Kizito
authors.affiliation: Diagnostic Center for Animal Hygiene and Food Safety, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Inada, Obihiro, Hokkaido 080-8555, Japan; Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Egypt.
authors.forename: Mohammed
authors.initials: M
authors.lastname: AboElkhair
authors.affiliation: Japan Livestock Technology Association, 3-20-9 Yushima, Bunkyo-ku, Tokyo 113-0034, Japan.
authors.forename: Shigeo
authors.initials: S
authors.lastname: Yamaguchi
authors.affiliation: National Institute of Veterinary Research, 86 Truong Chinh, Dong Da, Hanoi, Vietnam.
authors.forename: Viet Khong
authors.initials: VK
authors.lastname: Nguyen
authors.affiliation: Diagnostic Center for Animal Hygiene and Food Safety, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Inada, Obihiro, Hokkaido 080-8555, Japan. Electronic address: imaiku@obihiro.ac.jp.
authors.forename: Kunitoshi
authors.initials: K
authors.lastname: Imai
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D017727:Chicken anemia virus / Q000276:immunology / Q000302:isolation & purification*; D002645:Chickens; D018173:Circoviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D007841:Latex Fixation Tests / Q000379:methods*; D011201:Poultry Diseases / Q000175:diagnosis* / Q000821:virology*; D012680:Sensitivity and Specificity; D013997:Time Factors; D014730:Veterinary Medicine / Q000379:methods*
chemical_list: D000914:Antibodies, Viral
keywords: Blocking latex agglutination test; Chicken anemia virus; Monoclonal antibody; Serum antibody