title: Inhibition of Human Neutrophil Responses by the Essential Oil of Artemisia kotuchovii and Its Constituents.
abstract: Essential oils were obtained by hydrodistillation of the flowers+leaves and stems of Artemisia kotuchovii Kupr. (AKEO(f+l) and AKEO(stm), respectively) and analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The primary components of the oils were estragole, (E)- and (Z)-β-ocimenes, methyleugenol, limonene, spathulenol, β-pinene, myrcene, and (E)-methyl cinnamate. Seventy-four constituents were present at concentrations from 0.1 to 1.0%, and 34 compounds were identified in trace (<0.1%) amounts in one or both plant components. Screening of the essential oils for biological activity showed that AKEO(stm), but not AKEOf+l, inhibited N-formyl-Met-Leu-Phe (fMLF)-stimulated Ca(2+) flux and chemotaxis and phorbol-12-myristate-13-acetate (PMA)-induced reactive oxygen species (ROS) production in human neutrophils. Selected pure constituents, representing >96% of the AKEO(stm) composition, were also tested in human neutrophils and HL-60 cells transfected with N-formyl peptide receptor 1 (FPR1). One component, 6-methyl-3,5-heptadien-2-one (MHDO), inhibited fMLF- and interleukin 8 (IL-8)-stimulated Ca(2+) flux, fMLF-induced chemotaxis, and PMA-induced ROS production in human neutrophils. MHDO also inhibited fMLF-induced Ca(2+) flux in FPR1-HL60 cells. These results suggest that MHDO may be effective in modulating some innate immune responses, possibly by inhibition of neutrophil migration and ROS production.
authors.affiliation: †Department of Microbiology and Immunology, Montana State University, Bozeman, Montana 59717, United States.
authors.forename: Igor A
authors.initials: IA
authors.lastname: Schepetkin
authors.affiliation: §Institute of Plant Biology and Biotechnology, Almaty 050040, Republic of Kazakhstan.
authors.forename: Svetlana V
authors.initials: SV
authors.lastname: Kushnarenko
authors.affiliation: #Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, Eskisehir 26470, Turkey.
authors.forename: Gulmira
authors.initials: G
authors.lastname: Özek
authors.affiliation: †Department of Microbiology and Immunology, Montana State University, Bozeman, Montana 59717, United States.
authors.forename: Liliya N
authors.initials: LN
authors.lastname: Kirpotina
authors.affiliation: §Institute of Plant Biology and Biotechnology, Almaty 050040, Republic of Kazakhstan.|ΔFaculty of Biology and Biotechnology, Al-Farabi Kazakh National University, 71 al-Farabi Avenue, 050038 Almaty, Republic of Kazakhstan.
authors.forename: Gulzhakhan A
authors.initials: GA
authors.lastname: Utegenova
authors.affiliation: ⊥Altai Botanical Garden, 07130 Ridder, Republic of Kazakhstan.
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Kotukhov
authors.affiliation: ⊥Altai Botanical Garden, 07130 Ridder, Republic of Kazakhstan.
authors.forename: Alevtina N
authors.initials: AN
authors.lastname: Danilova
authors.affiliation: #Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, Eskisehir 26470, Turkey.
authors.forename: Temel
authors.initials: T
authors.lastname: Özek
authors.affiliation: #Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, Eskisehir 26470, Turkey.|○Botany and Microbiology Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
authors.forename: K Hüsnü Can
authors.initials: KH
authors.lastname: Başer
authors.affiliation: †Department of Microbiology and Immunology, Montana State University, Bozeman, Montana 59717, United States.
authors.forename: Mark T
authors.initials: MT
authors.lastname: Quinn
mesh_terms: D019306:Artemisia / Q000737:chemistry*; D002465:Cell Movement / Q000187:drug effects; D008401:Gas Chromatography-Mass Spectrometry; D018922:HL-60 Cells; D006801:Humans; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D009822:Oils, Volatile; D010936:Plant Extracts; D010938:Plant Oils
keywords: Artemisia kotuchovii; N-formyl peptide receptor 1 (FPR1); calcium flux; chemotaxis; essential oil; neutrophil; reactive oxygen species