title: Bone marrow-derived stromal cells are more beneficial cell sources for tooth regeneration compared with adipose-derived stromal cells.
abstract: Tooth loss is presently a global epidemic and tooth regeneration is thought to be a feasible and ideal treatment approach. Choice of cell source is a primary concern in tooth regeneration. In this study, the odontogenic differentiation potential of two non-dental-derived stem cells, adipose-derived stromal cells (ADSCs) and bone marrow-derived stromal cells (BMSCs), were evaluated both in vitro and in vivo. ADSCs and BMSCs were induced in vitro in the presence of tooth germ cell-conditioned medium (TGC-CM) prior to implantation into the omentum majus of rats, in combination with inactivated dentin matrix (IDM). Real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect the mRNA expression levels of odontogenic-related genes. Immunofluorescence and immunohistochemical assays were used to detect the protein levels of odontogenic-specific genes, such as DSP and DMP-1 both in vitro and in vivo. The results suggest that both ADSCs and BMSCs have odontogenic differentiation potential. However, the odontogenic potential of BMSCs was greater compared with ADSCs, showing that BMSCs are a more appropriate cell source for tooth regeneration.
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, P. R. China.
authors.forename: Lanfeng
authors.initials: L
authors.lastname: Ye
authors.affiliation: Department of Oral and Maxillofacial Surgery, The Second Xiangya Hospital, Central South University, Changsha, 410011, P. R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.affiliation: Air Force Dujiangyan Aviation Medicine Evaluation and Train Center, No. 18, Juqing Road, Dujiangyan, Chengdu, 611830, Sichuan, P. R. China.
authors.forename: Fan
authors.initials: F
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, P. R. China.
authors.forename: Junhui
authors.initials: J
authors.lastname: Cui
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, P. R. China.
authors.forename: Kaide
authors.initials: K
authors.lastname: Li
authors.affiliation: Department of Oral & Maxillofacial surgery, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, 310006, P. R. China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Li
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, P. R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology*; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D051381:Rats; D012038:Regeneration; D017154:Stromal Cells / Q000502:physiology; D014070:Tooth / Q000502:physiology*
keywords: adipose-derived stromal cells; bone marrow-derived stromal cells; inactivated dentin matrix; seedcell source; tooth germ cell-conditioned medium; tooth regeneration