title: Incorporation of antimicrobial peptides on functionalized cotton gauzes for medical applications.
abstract: A large group of low molecular weight natural compounds that exhibit antimicrobial activity has been isolated from animals and plants during the past two decades. Among them, peptides are the most widespread resulting in a new generation of antimicrobial agents with higher specific activity. In the present study we have developed a new strategy to obtain antimicrobial wound-dressings based on the incorporation of antimicrobial peptides into polyelectrolyte multilayer films built by the alternate deposition of polycation (chitosan) and polyanion (alginic acid sodium salt) over cotton gauzes. Energy dispersive X ray microanalysis technique was used to determine if antimicrobial peptides penetrated within the films. FTIR analysis was performed to assess the chemical linkages, and antimicrobial assays were performed with two strains: Staphylococcus aureus (Gram-positive bacterium) and Klebsiella pneumonia (Gram-negative bacterium). Results showed that all antimicrobial peptides used in this work have provided a higher antimicrobial effect (in the range of 4 log-6 log reduction) for both microorganisms, in comparison with the controls, and are non-cytotoxic to normal human dermal fibroblasts at the concentrations tested.
authors.affiliation: FibEnTech - Fiber Materials and Environmental Technologies - Research Unit, Faculty of Engineering, University of Beira Interior, 6201-001 Covilhã, Portugal.
authors.forename: A P
authors.initials: AP
authors.lastname: Gomes
authors.affiliation: 3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Taipas, Guimarães, Portugal; Health Sciences Research Centre, University of Beira Interior, 6201-001 Covilhã, Portugal.
authors.forename: J F
authors.initials: JF
authors.lastname: Mano
authors.affiliation: ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal.
authors.forename: J A
authors.initials: JA
authors.lastname: Queiroz
authors.affiliation: FibEnTech - Fiber Materials and Environmental Technologies - Research Unit, Faculty of Engineering, University of Beira Interior, 6201-001 Covilhã, Portugal. Electronic address: igouveia@ubi.pt.
authors.forename: I C
authors.initials: IC
authors.lastname: Gouveia
mesh_terms: D000464:Alginates / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000494:pharmacology*; D002470:Cell Survival; D048271:Chitosan / Q000737:chemistry; D032561:Cotton Fiber; D004573:Electrolytes / Q000737:chemistry; D005347:Fibroblasts / Q000166:cytology; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000254:growth & development; D008826:Microbial Sensitivity Tests; D012867:Skin / Q000166:cytology; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development; D013329:Structure-Activity Relationship
chemical_list: D000464:Alginates; D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D004573:Electrolytes; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D048271:Chitosan
keywords: Antimicrobial peptides; Biocompatibility; Cys-LC-LL-37; Dermaseptin; Human; Magainin 1; Wound-dressing; hBD-1; β-Defensin-1