title: Thymic derived iPs cells can be differentiated into cardiomyocytes.
abstract: Ventricular septal defect (VSD) is one of the common congenital heart malformations. Several factors lead to the development of VSD, including familial causes, exposure to certain drugs, infectious agents, and maternal metabolic disturbances. We considered that induced pluripotent stem (iPS) cells derived from VSD patients can be used to study the origin and pathogenesis of the VSD. Here, we show generation and cardiomyocyte differentiation potential of iPS cells from thymic epithelial cells of a patient with VSD (TECs-VSD) by overexpressing the four factors: OCT4, SOX2, NANOG, and LIN28 with lentiviral vectors. The self-renewal and pluripotency of the VSD-iPS cells was verified in iPS cells by in vitro expression of pluripotency markers and formation of teratoma in vivo. iPS cell lines from VSD patients differentiated into functional cardiomyocytes can serve as a model system for studying the pathophysiology and identifying etiology of VSD.
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.forename: Yin-yin
authors.initials: YY
authors.lastname: Cao
authors.forename: Xiao-jing
authors.initials: XJ
authors.lastname: Ma
authors.affiliation: Children's Hospital of Fudan University, Shanghai 201102, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.affiliation: Children's Hospital of Fudan University, Shanghai 201102, China.
authors.forename: Shuo-lin
authors.initials: SL
authors.lastname: Li
authors.affiliation: Department of Chemistry, Yangzhou University, Yangzhou 225002, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Gao
authors.forename: Hui-jun
authors.initials: HJ
authors.lastname: Wang
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan, 410083, P. R. China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Institute of Biomedical Science, Fudan University, Shanghai 200032, China.
authors.forename: Duan
authors.initials: D
authors.lastname: Ma
authors.affiliation: Children's Hospital of Fudan University, Shanghai 201102, China, gyhuang@shmu.edu.cn.
authors.forename: Guo-ying
authors.initials: GY
authors.lastname: Huang
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002460:Cell Line; D006345:Heart Septal Defects, Ventricular / Q000473:pathology; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D008815:Mice, Inbred Strains; D032383:Myocytes, Cardiac / Q000166:cytology*; D013950:Thymus Gland / Q000166:cytology