title: Reptin physically interacts with p65 and represses NF-κB activation.
abstract: Reptin and Pontin belong to the AAA+ ATPase family of DNA helicases. Both proteins are present in several chromatin-remodeling machineries and are involved in transcriptional regulation, DNA repair, and telomerase activity, but they also function independently from each other. Here we report the identification of p65 as an interacting partner of Reptin. Using reporter gene assays, we show Reptin inhibits NF-κB transactivation after TNFα stimulation. Reptin is mainly localized in the cytoplasm and impedes NF-κB activation by inhibiting IκB-α degradation and restraining p65 nuclear translocation. These results reveal a novel mechanism for the control of NF-κB pathway by cytoplasmic Reptin.
authors.affiliation: The First Affiliated Hospital of Guangxi University of Chinese Medicine, 89-9 Dongge Road, Nanning, Guangxi 530023, China; ShuGuang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, 528 Zhangheng Road, Pudong New Area, Shanghai 201203, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Qiu
authors.affiliation: ShuGuang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, 528 Zhangheng Road, Pudong New Area, Shanghai 201203, China. Electronic address: gaoyueqiu@hotmail.com.
authors.forename: Yueqiu
authors.initials: Y
authors.lastname: Gao
authors.affiliation: The First Affiliated Hospital of Guangxi University of Chinese Medicine, 89-9 Dongge Road, Nanning, Guangxi 530023, China. Electronic address: mdwboshi2005@sina.com.
authors.forename: Dewen
authors.initials: D
authors.lastname: Mao
mesh_terms: D000074183:ATPases Associated with Diverse Cellular Activities; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D006801:Humans; D011485:Protein Binding; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D051996:Transcription Factor RelA / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D002352:Carrier Proteins; D034741:RNA, Small Interfering; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha; D000074183:ATPases Associated with Diverse Cellular Activities; D004265:DNA Helicases; C122079:RUVBL2 protein, human
keywords: Cytoplasma; NF-κB; Reptin; Transcription