title: Circulating microRNAs as novel prognosis biomarkers for head and neck squamous cell carcinoma.
abstract: Circulating microRNAs (miRNAs) are emerging as promising non-invasive biomarkers for human cancer. Head and neck squamous cell carcinoma (HNSCC) is a prevalent malignancy worldwide, but its overall survival has remained unchanged in the past 3 decades. Biomarkers for evaluating efficacy of cancer therapy are urgently needed. To explore circulating miRNAs as cancer therapy biomarkers, we initially identified that 8 miRNAs were distinctly dysregulated in cancerous tissues compared with adjacent non-cancerous counterparts from 16 patients, using microarray and real-time PCR. Based on this discovery, the comparison study was performed between pre- and 6 months post-operative paired plasma samples on 9 patients. MiR-99a, which was down-regulated in cancerous tissues, was significantly increased in plasma after operation. Meanwhile, oncomiR miR-21 and miR-223 that were up-regulated in cancerous tissues, were significantly reduced in post-operative plasma samples. We firstly report the significant changes of miR-99a in plasma of HNSCC patients after surgery. Furthermore, plasma miR-223 was inversely increased in a patient whose cancer relapsed within 6 months after operation. We conclude that these circulating miRNAs may serve as biomarkers to evaluate the efficacy of therapy and the prognosis of HNSCC.
authors.affiliation: a Department of Otolaryngology-Head and Neck Surgery ; Tsu , Japan.
authors.forename: Bo
authors.initials: B
authors.lastname: Hou
authors.forename: Hajime
authors.initials: H
authors.lastname: Ishinaga
authors.forename: Kaoru
authors.initials: K
authors.lastname: Midorikawa
authors.forename: Said Ahmad
authors.initials: SA
authors.lastname: Shah
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nakamura
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Hiraku
authors.forename: Shinji
authors.initials: S
authors.lastname: Oikawa
authors.forename: Mariko
authors.initials: M
authors.lastname: Murata
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Takeuchi
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000235:genetics* / Q000601:surgery; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D006258:Head and Neck Neoplasms / Q000097:blood / Q000235:genetics* / Q000601:surgery; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D011184:Postoperative Period; D011379:Prognosis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; C524940:MIRN21 microRNA, human; C525067:MIR223, human; C561679:MIRN99 microRNA, human
keywords: HNSCC, head and neck squamous cell carcinoma; biomarker; circulating miRNA; head and neck squamous cell carcinoma; miR-223; miR-99a; miRNA, microRNA