title: Response to Brosch et al.
abstract: We would like to respond to Brosch et al. regarding our manuscript "Expression of the Splicing Factor GeneSFRS10Is Reduced in Human Obesity and Contributes to Enhanced Lipogenesis" (Pihlajamäki et al., 2011b). Brosch performed RT-PCR in liver samples from 13 lean and 34 obese individuals, finding no differences inSFRS10orLPIN1expression. We wish to address points raised by Brosch, including experimental strategy and analysis of humanSFRS10expression.
authors.affiliation: Research Division, Joslin Diabetes Center, Harvard Medical School, Boston, MA 02215, USA ; Department of Medicine, University of Eastern Finland, Kuopio 70211, Finland ; Department of Clinical Nutrition, University of Eastern Finland, Kuopio 70211, Finland.
authors.forename: Jussi
authors.initials: J
authors.lastname: Pihlajamäki
authors.affiliation: Research Division, Joslin Diabetes Center, Harvard Medical School, Boston, MA 02215, USA ; Diabetes and Obesity Laboratory, IDIBAPS-CIBERDEM, 08036 Barcelona, Spain.
authors.forename: Carles
authors.initials: C
authors.lastname: Lerin
authors.affiliation: Department of Clinical Nutrition, University of Eastern Finland, Kuopio 70211, Finland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Kaminska
authors.affiliation: Department of Medicine, University of Eastern Finland, Kuopio 70211, Finland.
authors.forename: Sari
authors.initials: S
authors.lastname: Venesmaa
authors.affiliation: Department of Medicine, University of Eastern Finland, Kuopio 70211, Finland.
authors.forename: Paula
authors.initials: P
authors.lastname: Itkonen
authors.affiliation: Research Division, Joslin Diabetes Center, Harvard Medical School, Boston, MA 02215, USA.
authors.forename: Tanner
authors.initials: T
authors.lastname: Boes
authors.affiliation: Helmholtz Zentrum München, Technische Universität München, Institut für Entwicklungsgenetik, Ingolstädter Landstrasse 1, 85764 Munich, Neuherberg, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Floss
authors.affiliation: Research Division, Joslin Diabetes Center, Harvard Medical School, Boston, MA 02215, USA.
authors.forename: Joshua
authors.initials: J
authors.lastname: Schroeder
authors.affiliation: Research Division, Joslin Diabetes Center, Harvard Medical School, Boston, MA 02215, USA.
authors.forename: Farrell
authors.initials: F
authors.lastname: Dearie
authors.affiliation: Research Division, Joslin Diabetes Center, Harvard Medical School, Boston, MA 02215, USA.
authors.forename: Sarah
authors.initials: S
authors.lastname: Crunkhorn
authors.affiliation: Research Division, Joslin Diabetes Center, Harvard Medical School, Boston, MA 02215, USA.
authors.forename: Furkan
authors.initials: F
authors.lastname: Burak
authors.affiliation: Research Division, Joslin Diabetes Center, Harvard Medical School, Boston, MA 02215, USA.
authors.forename: Josep C
authors.initials: JC
authors.lastname: Jimenez-Chillaron
authors.affiliation: Department of Medicine, University of Eastern Finland, Kuopio 70211, Finland.
authors.forename: Tiina
authors.initials: T
authors.lastname: Kuulasmaa
authors.affiliation: Department of Surgery, University of Eastern Finland, Kuopio 70211, Finland.
authors.forename: Pekka
authors.initials: P
authors.lastname: Miettinen
authors.affiliation: Children's Hospital, Harvard Medical School, Boston, MA 02215, USA.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Park
authors.affiliation: Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors.forename: Imad
authors.initials: I
authors.lastname: Nasser
authors.affiliation: Department of Obstetrics and Gynecology, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
authors.forename: Zhenwen
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY 40536, USA.
authors.forename: Zhaiyi
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and Gynecology, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Helmholtz Zentrum München, Technische Universität München, Institut für Entwicklungsgenetik, Ingolstädter Landstrasse 1, 85764 Munich, Neuherberg, Germany ; MPI für Psychiatrie, Kraepelinstrasse 2-10, 80804 München, and Technical University Weihenstephan, Lehrstuhl für Entwicklungsgenetik, c/o Helmholtz Zentrum München, and DZNE-site Munich, c/o Adolf Butenandt Institute, LMU, Schillerstrasse 44, 80336 Munich, Germany.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Wurst
authors.affiliation: Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY 40536, USA.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Ren
authors.affiliation: Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY 40536, USA.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Morris
authors.affiliation: Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY 40536, USA.
authors.forename: Stefan
authors.initials: S
authors.lastname: Stamm
authors.affiliation: Research Division, Joslin Diabetes Center, Harvard Medical School, Boston, MA 02215, USA.
authors.forename: Allison B
authors.initials: AB
authors.lastname: Goldfine
authors.affiliation: Department of Medicine, University of Eastern Finland, Kuopio 70211, Finland.
authors.forename: Markku
authors.initials: M
authors.lastname: Laakso
authors.affiliation: Research Division, Joslin Diabetes Center, Harvard Medical School, Boston, MA 02215, USA.
authors.forename: Mary Elizabeth
authors.initials: ME
authors.lastname: Patti