title: A survey of the venom of the spider Lycosa vittata by biochemical, pharmacological and transcriptomic analyses.
abstract: Lycosa vittata, mainly distributed in the southwest of China, is a medium-sized and venomous spider, whose venom remains unexplored so far. This study aims to present an overview of the venom. It mainly consisted of diverse peptides and exhibited inhibitory effects on voltage-gated ion channels in rat dorsal root ganglia neurons, with a strongest inhibition on tetrodotoxin-sensitive and tetrodotoxin-resistant voltage-gated Na(+) channels. Interestingly, it exerted cytotoxicity to cancer cells, with approximately 10-fold selectivity on PC-3 over others, implying the existence of selective anti-PC-3 agents in the venom. Moreover, 51 toxin-like peptides were deduced from the venom gland transcriptome. Bioinformatic analyses suggested their structures might have some distinguished properties and their predicted functions were consistent with the venom activities. This study suggests that the venom is an attractive source of neurotoxins with therapeutic significance, and provides references for the structure and function investigation of specific toxins in the future.
authors.affiliation: College of Life Sciences, Hunan Normal University, Changsha, 410081, Hunan, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Research Center of Biological Information, College of Science, National University of Defense Technology, Changsha, Hunan, 410073, China.
authors.forename: Changjun
authors.initials: C
authors.lastname: Liu
authors.affiliation: College of Life Sciences, Hunan Normal University, Changsha, 410081, Hunan, China.
authors.forename: Huaxin
authors.initials: H
authors.lastname: Tan
authors.affiliation: College of Life Sciences, Hunan Normal University, Changsha, 410081, Hunan, China.
authors.forename: Hengyun
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Hunan Normal University, Changsha, 410081, Hunan, China.
authors.forename: Yinjie
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: College of Life Sciences, Hunan Normal University, Changsha, 410081, Hunan, China.
authors.forename: Songping
authors.initials: S
authors.lastname: Liang
authors.affiliation: Agriculture and Animal Husbandry College, Tibet University, Linzhi, 860000, China. Electronic address: zhfp2114@sina.com.
authors.forename: Fuping
authors.initials: F
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Hunan Normal University, Changsha, 410081, Hunan, China. Electronic address: liuzh@hunnu.edu.cn.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000737:chemistry*; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D057809:HEK293 Cells; D006801:Humans; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010802:Phylogeny; D051381:Rats; D016415:Sequence Alignment; D012984:Software; D013111:Spider Venoms / Q000737:chemistry*; D013112:Spiders / Q000378:metabolism*; D059467:Transcriptome; D020298:U937 Cells
chemical_list: D060829:Arthropod Proteins; D013111:Spider Venoms
keywords: Bioactivity; Lycosa vittata; Peptide toxins; Spider venom; Transcriptome