title: Antibacterial and Antiproliferative Activities of Plumericin, an Iridoid Isolated from Momordica charantia Vine.
abstract: Plumericin, an iridoid lactone, was isolated with relatively high yield from Momordica charantia vine using the supercritical fluid extraction (SFE) and the separation box (Sepbox) comprising dual combination of high-performance liquid chromatography and solid phase extraction. This compound showed antibacterial activity against Enterococcus faecalis and Bacillus subtilis with minimum inhibitory concentration (MIC) values better than cloxacillin. Plumericin potently inhibited proliferation of two leukemic cancer cell lines: they were acute and chronic leukemic cancer cell lines, NB4 and K562, with the effective doses (ED50) of 4.35 ± 0.21 and 5.58 ± 0.35 μg/mL, respectively. In addition, the mechanism of growth inhibition in both cell lines was induced by apoptosis, together with G2/M arrest in K562 cells.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, 447 Sri-Ayudhya Road, Rajthevi, Bangkok 10400, Thailand.
authors.forename: Jutamas
authors.initials: J
authors.lastname: Saengsai
authors.affiliation: Department of Biotechnology, Faculty of Applied Science, King Mongkut's University of Technology North Bangkok, Bangsue, Bangkok 10800, Thailand.
authors.forename: Sumonthip
authors.initials: S
authors.lastname: Kongtunjanphuk
authors.affiliation: Department of Chemical Engineering, Faculty of Engineering, Mahidol University, 25/25 Puttamonthon 4 Road, Salaya, Nakhon Pathom 73170, Thailand.
authors.forename: Nuttawan
authors.initials: N
authors.lastname: Yoswatthana
authors.affiliation: Department of Clinical Pathology, Faculty of Medicine Siriraj Hospital, 2 Prannok Road, Bangkoknoi, Bangkok 10700, Thailand.
authors.forename: Tanawan
authors.initials: T
authors.lastname: Kummalue
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, 447 Sri-Ayudhya Road, Rajthevi, Bangkok 10400, Thailand.
authors.forename: Weena
authors.identifier: 0000-0001-9918-2534
authors.initials: W
authors.lastname: Jiratchariyakul