title: Cysteine-rich receptor-like kinase CRK5 as a regulator of growth, development, and ultraviolet radiation responses in Arabidopsis thaliana.
abstract: In plants, receptor-like protein kinases play essential roles in signal transduction by recognizing extracellular stimuli and activating the downstream signalling pathways. Cysteine-rich receptor-like kinases (CRKs) constitute a large subfamily of receptor-like protein kinases, with 44 members in Arabidopsis thaliana. They are distinguished by the novel C-X8-C-X2-C motif (DUF26) in the extracellular domains. One of them, CRK5, is an important component of the biochemical machinery involved in the regulation of essential physiological processes. Functional characterization of crk5 mutant plants showed their clear phenotype, manifested by impaired stomatal conductance and accelerated senescence. This phenotype correlated with accumulation of reactive oxygen species, higher foliar levels of ethylene and salicylic acid, and increased transcript abundance for genes associated with signalling pathways corresponding to these hormones. Moreover, the crk5 plants displayed enhanced cell death and oxidative damage in response to ultraviolet radiation. Complementation of CRK5 mutation managed to recover the wild-type phenotype, indicating an essential role of this gene in the regulation of growth, development, and acclimatory responses.
authors.affiliation: Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences - SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland.
authors.forename: Paweł
authors.initials: P
authors.lastname: Burdiak
authors.affiliation: Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences - SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Rusaczonek
authors.affiliation: Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences - SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland.
authors.forename: Damian
authors.initials: D
authors.lastname: Witoń
authors.affiliation: Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences - SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland.
authors.forename: Dawid
authors.initials: D
authors.lastname: Głów
authors.affiliation: Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences - SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland stanislaw_karpinski@sggw.pl.
authors.forename: Stanisław
authors.initials: S
authors.lastname: Karpiński
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D016923:Cell Death; D003545:Cysteine / Q000378:metabolism; D005030:Ethylenes / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D009154:Mutation; D018384:Oxidative Stress; D010641:Phenotype; D010788:Photosynthesis; D010937:Plant Growth Regulators / Q000378:metabolism*; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development / Q000528:radiation effects; D054046:Plant Stomata / Q000235:genetics / Q000254:growth & development / Q000528:radiation effects; D030821:Plants, Genetically Modified; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism; D020156:Salicylic Acid / Q000378:metabolism; D036226:Seedlings / Q000235:genetics / Q000254:growth & development / Q000528:radiation effects; D015398:Signal Transduction; D014466:Ultraviolet Rays
chemical_list: D029681:Arabidopsis Proteins; D005030:Ethylenes; D010937:Plant Growth Regulators; D017382:Reactive Oxygen Species; D011956:Receptors, Cell Surface; D002245:Carbon Dioxide; C036216:ethylene; C420215:CRK5 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases; D003545:Cysteine; D020156:Salicylic Acid
keywords: Cell death; cysteine-rich kinases (CRKs); ethylene (ET); reactive oxygen species (ROS); salicylic acid (SA); senescence.