title: The Nrf2-ARE pathway is associated with Schisandrin b attenuating benzo(a)pyrene-Induced HTR cells damages in vitro.
abstract: As is ubiquitous in the environmental sources, benzo(a)pyrene (BaP) has been reported to induce reprotoxicity in previous studies. Toxicity to trophoblast cells may be one key factor, but evidences were absent. We speculated that BaP can induce cytotoxicity in human trophoblast HTR-8/SVneo (HTR) cells, and Schisandrin B (Sch B) as a potential protector can inhibit the cytotoxicity. MTS assay identified that BaP induced HTR cells death while Sch B played a cytoprotective role. And after Nrf2 interference, the ability of Sch B-induced cytoprotection was declined. Furthermore, PCR, western blot, ELISA, and SOD assays were found that Sch B significantly increased the mRNA and protein expression of Nrf2, HO1, NQO1, and SOD in the Nrf2-ARE pathway, and the extents of increase were declined after Nrf2 interference. These results demonstrated that the Nrf2-ARE pathway plays an important role in Sch B attenuating BaP-induced HTR cells damages in vitro. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1439-1449, 2016.
authors.affiliation: Department of Obstetrics and Gynecology, Affiliated Hospital of Logistics University of the Chinese People's Armed Forces, Tianjin, 300162, China.
authors.forename: Qulong
authors.initials: Q
authors.lastname: Dong
authors.affiliation: Department of Obstetrics and Gynecology, Affiliated Hospital of Logistics University of the Chinese People's Armed Forces, Tianjin, 300162, China.|Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100730, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Hou
authors.affiliation: Program in Public Health and Department of Epidemiology, University of California, Irvine, 92697, USA.
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Obstetrics and Gynecology, Affiliated Hospital of Logistics University of the Chinese People's Armed Forces, Tianjin, 300162, China. chenyq82@hotmail.com.|Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazard, Tianjin, 300162, China. chenyq82@hotmail.com.
authors.forename: Yaqiong
authors.initials: Y
authors.lastname: Chen
mesh_terms: D061745:Antioxidant Response Elements / Q000187:drug effects; D001564:Benzo(a)pyrene / Q000037:antagonists & inhibitors* / Q000633:toxicity; D002460:Cell Line; D034242:Cyclooctanes / Q000494:pharmacology; D019610:Cytoprotection; D005260:Female; D006801:Humans; D017705:Lignans / Q000494:pharmacology*; D051267:NF-E2-Related Factor 2 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D011083:Polycyclic Compounds / Q000494:pharmacology*; D034741:RNA, Small Interfering / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D014327:Trophoblasts / Q000187:drug effects* / Q000502:physiology
chemical_list: D034242:Cyclooctanes; D017705:Lignans; D051267:NF-E2-Related Factor 2; D011083:Polycyclic Compounds; D034741:RNA, Small Interfering; C015499:schizandrin B; D001564:Benzo(a)pyrene
keywords: HTR; Nrf2-ARE pathway; Schisandrin B; benzo(a)pyrene