title: Micromonospora endophytica sp. nov., an endophytic actinobacteria of Thai upland rice (Oryza sativa).
abstract: An actinobacterial strain, DCWR9-8-2(T), was isolated from a leaf of Thai upland rice (Oryza sativa) collected in Chumporn province, Thailand. Strain DCWR9-8-2(T) is Gram-stain-positive aerobic bacteria that produce single spores directly on the vegetative hypha. Cell wall peptidoglycan of this strain exhibits meso-diaminopimelic acid and glycine, the reducing sugars of whole-cell hydrolysate are arabinose, glucose, ribose, xylose and small amount of mannose. The phospholipid profiles in the membrane are comprised of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides. The major menaquinones are MK-9(H4) and MK-10(H6). The diagnostic cellular fatty acids are iso-C16:0 and iso-C15:0. The G+C content of the genomic DNA is 72.5 mol%. The result of 16S rRNA sequence analysis of the strain revealed that this strain was closely related to Micromonospora auratinigra TT1-11(T) (99.25%). On the other hand, the result of gyrB gene sequence analysis revealed that this strain was closed to M. eburnea JCM 12345(T) (96.30%). In addition, a combination of DNA-DNA hybridization results and some phenotypic properties supported that this strain should be judged as a novel species of the genus Micromonospora, for which the name M. endophytica sp. nov. is proposed. The type strain is DCWR9-8-2(T) (=BCC 67267(T)=NBRC 110008(T)).
authors.affiliation: Department of Biology, King Mongkut's Institute of Technology Ladkrabang (KMITL), Bangkok, Thailand.
authors.forename: Dusanee
authors.initials: D
authors.lastname: Thanaboripat
authors.affiliation: Department of Biology, King Mongkut's Institute of Technology Ladkrabang (KMITL), Bangkok, Thailand.|Actinobacterial Research Unit, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang (KMITL), Bangkok, Thailand.
authors.forename: Chitti
authors.initials: C
authors.lastname: Thawai
authors.affiliation: Department of Biology, King Mongkut's Institute of Technology Ladkrabang (KMITL), Bangkok, Thailand.|Actinobacterial Research Unit, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang (KMITL), Bangkok, Thailand.
authors.forename: Chokchai
authors.initials: C
authors.lastname: Kittiwongwattana
authors.affiliation: Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang (KMITL), Bangkok, Thailand.
authors.forename: Chamroon
authors.initials: C
authors.lastname: Laosinwattana
authors.affiliation: Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang (KMITL), Bangkok, Thailand.
authors.forename: Prommart
authors.initials: P
authors.lastname: Koohakan
authors.affiliation: Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang (KMITL), Bangkok, Thailand.
authors.forename: Nonglak
authors.initials: N
authors.lastname: Parinthawong
mesh_terms: D008847:Micromonospora / Q000737:chemistry / Q000235:genetics / Q000254:growth & development*; D012275:Oryza / Q000382:microbiology*; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D010743:Phospholipids; D012336:RNA, Ribosomal, 16S