title: Tumor Necrosis Factor-α Underlies Loss of Cortical Dendritic Spine Density in a Mouse Model of Congestive Heart Failure.
abstract: BACKGROUND
  Heart failure (HF) is a progressive disorder characterized by reduced cardiac output and increased peripheral resistance, ultimately leading to tissue perfusion deficits and devastating consequences for several organs including the brain. We previously described a tumor necrosis factor-α (TNF-α)-dependent enhancement of posterior cerebral artery tone and concomitant reduced cerebral blood flow in a mouse model of early HF in which blood pressure remains minimally affected. HF is often associated with cognitive impairments such as memory deficits, even before any overt changes in brain structure and function occur. The pathophysiology underlying the development of cognitive impairments in HF is unknown, and appropriate treatment strategies are lacking.
  
  
  RESULTS
  We used a well-established mouse model in which HF was induced by experimental myocardial infarction produced by permanent surgical ligation of the left anterior descending coronary artery (infarct size ≈25% of the left ventricular wall). Ligated mice developed enlarged hearts, congested lungs, and reduced cardiac output and blood pressure, with elevated peripheral resistance within 6 to 8 weeks after ligation. In this study, we demonstrated the significance of the proinflammatory cytokine TNF-α during HF-mediated neuroinflammation and associated impaired hippocampus-independent nonspatial episodic memory function. Augmented cerebral TNF-α expression and microglial activation in HF mice, indicative of brain inflammation, were accompanied by morphological changes and significant reduction of cortical dendritic spines (61.39±8.61% for basal and 61.04±9.18% for apical spines [P<0.001]). The significance of TNF-α signaling during the observed HF-mediated neurodegenerative processes is supported by evidence showing that sequestration or genetic deletion of TNF-α ameliorates the observed reduction of cortical dendritic spines (33.51±7.63% for basal and 30.13±6.98% for apical spines in wild-type mice treated with etanercept; 17.09±6.81% for basal and 17.21±7.29% for apical spines in TNF-α(-/-)). Moreover, our data suggest that alterations in cerebral serum and glucocorticoid-inducible kinase 1 (SgK1) expression and phosphorylation during HF may be TNF-α dependent and that an increase of SgK1 phosphorylation potentially plays a role in the HF-associated reduction of dendritic spine density.
  
  
  CONCLUSIONS
  Our findings demonstrate that TNF-α plays a pivotal role in HF-mediated neuroinflammation and associated alterations of cortical dendritic spine density and has the potential to reveal novel treatment strategies for HF-associated memory deficits.
authors.affiliation: Department of Physiology, University of Toronto, Ontario, Canada (A.M., S.S.B.) Department of Brain Ischemia and Neurodegeneration, Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS), Barcelona, Spain (A.M.).
authors.forename: Anja
authors.initials: A
authors.lastname: Meissner
authors.affiliation: Morten and Gloria Shulman Movement Disorders Centre, Toronto Western Hospital, Toronto, Ontario, Canada (N.P.V.).
authors.forename: Naomi P
authors.initials: NP
authors.lastname: Visanji
authors.affiliation: Division of Cell and Molecular Biology, Toronto General Hospital Research Institute, Toronto, Ontario, Canada (A.M.).
authors.forename: M Abdul
authors.initials: MA
authors.lastname: Momen
authors.affiliation: Tanz Center for Research in Neurodegenerative Diseases, Toronto, Ontario, Canada (R.F., B.M.F., L.N.H.).
authors.forename: Rui
authors.initials: R
authors.lastname: Feng
authors.affiliation: Tanz Center for Research in Neurodegenerative Diseases, Toronto, Ontario, Canada (R.F., B.M.F., L.N.H.).
authors.forename: Beverly M
authors.initials: BM
authors.lastname: Francis
authors.affiliation: Department of Physiology, University of Toronto, Ontario, Canada (A.M., S.S.B.) Heart and Stroke/Richard Lewar Centre of Excellence in Cardiovascular Research, Toronto, Ontario, Canada (S.S.B.) Toronto Centre for Microvascular Medicine, University of Toronto, and Li Ka Shing Knowledge Institute at St Michael's Hospital, Toronto, Ontario, Canada (S.S.B.).
authors.forename: Steffen-Sebastian
authors.initials: SS
authors.lastname: Bolz
authors.affiliation: Tanz Center for Research in Neurodegenerative Diseases, Toronto, Ontario, Canada (R.F., B.M.F., L.N.H.).
authors.forename: Lili-Naz
authors.initials: LN
authors.lastname: Hazrati
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D002540:Cerebral Cortex / Q000276:immunology / Q000473:pathology* / Q000503:physiopathology; D016207:Cytokines / Q000032:analysis; D049229:Dendritic Spines / Q000276:immunology / Q000473:pathology*; D004195:Disease Models, Animal; D004660:Encephalitis / Q000276:immunology* / Q000523:psychology; D006333:Heart Failure / Q000150:complications* / Q000276:immunology / Q000523:psychology; D017874:Immediate-Early Proteins / Q000032:analysis; D008569:Memory Disorders / Q000209:etiology* / Q000276:immunology / Q000473:pathology / Q000523:psychology; D061212:Memory, Episodic; D051379:Mice; D008810:Mice, Inbred C57BL; D017628:Microglia / Q000276:immunology; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000032:analysis; D012307:Risk Factors; D015398:Signal Transduction / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*; D000099184:Serum-Glucocorticoid Regulated Kinases
chemical_list: D016207:Cytokines; D017874:Immediate-Early Proteins; D017346:Protein Serine-Threonine Kinases; D014409:Tumor Necrosis Factor-alpha; D000099184:Serum-Glucocorticoid Regulated Kinases
keywords: heart failure; inflammation; nervous system