title: Analysis of class I and II histone deacetylase gene expression in human leukemia.
abstract: Histone deacetylase (HDAC) inhibitors are well-characterized anti-leukemia agents and HDAC gene expression deregulation has been reported in various types of cancers. This study sought to characterize HDAC gene expression patterns in several types of leukemia. To do so, a systematic study was performed of the mRNA expression of all drug-targetable HDACs for which reagents were available. This was done by real-time PCR in 24 leukemia cell lines and 39 leukemia patients, which included AML, MDS and CLL patients, some of whom received HDAC inhibitor treatment. Among the samples analyzed, there was no discernible pattern in HDAC expression. HDAC expression was generally increased in CLL patients, except for HDAC2 and HDAC4. HDAC expression was also generally increased in VPA-treated MOLT4 cells. However, this increased expression was not seen in AML patients treated with vorinostat. In summary, increased HDAC expression was noted in CLL patients in general, but the HDAC expression patterns in myeloid malignancies appear to be heterogeneous, which implies that the role of HDACs in leukemia may be related to global expression or protein function rather than specific expression patterns.
authors.affiliation: a Department of Leukemia , University of Texas MD Anderson Cancer Center , Houston , TX , USA.
authors.forename: Hui
authors.initials: H
authors.lastname: Yang
authors.affiliation: a Department of Leukemia , University of Texas MD Anderson Cancer Center , Houston , TX , USA.
authors.forename: Sirisha
authors.initials: S
authors.lastname: Maddipoti
authors.affiliation: a Department of Leukemia , University of Texas MD Anderson Cancer Center , Houston , TX , USA.
authors.forename: Andres
authors.initials: A
authors.lastname: Quesada
authors.affiliation: a Department of Leukemia , University of Texas MD Anderson Cancer Center , Houston , TX , USA.
authors.forename: Zachary
authors.initials: Z
authors.lastname: Bohannan
authors.affiliation: a Department of Leukemia , University of Texas MD Anderson Cancer Center , Houston , TX , USA.
authors.forename: Monica
authors.initials: M
authors.lastname: Cabrero Calvo
authors.affiliation: a Department of Leukemia , University of Texas MD Anderson Cancer Center , Houston , TX , USA.
authors.forename: Simona
authors.initials: S
authors.lastname: Colla
authors.affiliation: a Department of Leukemia , University of Texas MD Anderson Cancer Center , Houston , TX , USA.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wei
authors.affiliation: a Department of Leukemia , University of Texas MD Anderson Cancer Center , Houston , TX , USA.|b Department of Molecular Carcinogenesis , University of Texas MD Anderson Cancer Center , Houston , TX , USA.
authors.forename: Marcos
authors.initials: M
authors.lastname: Estecio
authors.affiliation: a Department of Leukemia , University of Texas MD Anderson Cancer Center , Houston , TX , USA.
authors.forename: William
authors.initials: W
authors.lastname: Wierda
authors.affiliation: c Department of Hematopathology , University of Texas MD Anderson Cancer Center , Houston , TX , USA.
authors.forename: Carlos
authors.initials: C
authors.lastname: Bueso-Ramos
authors.affiliation: a Department of Leukemia , University of Texas MD Anderson Cancer Center , Houston , TX , USA.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Garcia-Manero
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D020869:Gene Expression Profiling; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology; D006655:Histone Deacetylases / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007938:Leukemia / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D056572:Histone Deacetylase Inhibitors; D012333:RNA, Messenger; D006655:Histone Deacetylases
keywords: Histone; gene expression; histone deacetylase; histone deacetylase inhibitor; leukemia