title: Mycobacterium abscessus Complex Identification with Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry.
abstract: We determined that the Vitek MS Plus matrix-assisted laser desorption ionization-time of flight mass spectrometry using research-use-only (RUO) v.4.12 and in vitro-diagnostic (IVD) v.3.0 databases accurately identified 41 Mycobacterium abscessus subsp. abscessus and 13 M. abscessus subsp. massiliense isolates identified by whole-genome sequencing to the species but not the subspecies level, from Middlebrook 7H11 and Burkholderia cepacia selective agars. Peak analysis revealed three peaks potentially able to differentiate between subspecies.
authors.affiliation: Sismanogleio-A.Flemig General Hospital, Athens, Greece University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
authors.forename: Theofano
authors.initials: T
authors.lastname: Panagea
authors.affiliation: bioMérieux, Inc., Hazelwood, Missouri, USA.
authors.forename: David H
authors.initials: DH
authors.lastname: Pincus
authors.affiliation: Cambridge Centre for Lung Infection, Papworth Hospital, Cambridge, United Kingdom University of Cambridge, Cambridge, United Kingdom.
authors.forename: Dorothy
authors.initials: D
authors.lastname: Grogono
authors.affiliation: UNC Health Care, Chapel Hill, North Carolina, USA.
authors.forename: Melissa
authors.initials: M
authors.lastname: Jones
authors.affiliation: Wellcome Trust Sanger Institute, Hinxton, United Kingdom.
authors.forename: Josephine
authors.identifier: http://orcid.org/0000-0002-0149-5768
authors.initials: J
authors.lastname: Bryant
authors.affiliation: Wellcome Trust Sanger Institute, Hinxton, United Kingdom.
authors.forename: Julian
authors.identifier: http://orcid.org/0000-0002-7069-5958
authors.initials: J
authors.lastname: Parkhill
authors.affiliation: Cambridge Centre for Lung Infection, Papworth Hospital, Cambridge, United Kingdom Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom.
authors.forename: R Andres
authors.initials: RA
authors.lastname: Floto
authors.affiliation: University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA UNC Health Care, Chapel Hill, North Carolina, USA gilliganncphd@gmail.com.
authors.forename: Peter
authors.initials: P
authors.lastname: Gilligan
mesh_terms: D006801:Humans; D008828:Microbiological Techniques / Q000379:methods*; D009165:Mycobacterium Infections, Nontuberculous / Q000175:diagnosis / Q000382:microbiology; D009170:Nontuberculous Mycobacteria / Q000737:chemistry* / Q000145:classification*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*