title: Inducible and combinatorial gene manipulation in mouse brain.
abstract: We have deployed recombinant adeno-associated viruses equipped with tetracycline-controlled genetic switches to manipulate gene expression in mouse brain. Here, we show a combinatorial genetic approach for inducible, cell type-specific gene expression and Cre/loxP mediated gene recombination in different brain regions. Our chemical-genetic approach will help to investigate 'when', 'where', and 'how' gene(s) control neuronal circuit dynamics, and organize, for example, sensory signal processing, learning and memory, and behavior.
authors.affiliation: Department of Molecular Neurobiology, Max Planck Institute for Medical Research Heidelberg, Germany ; Institute of Experimental and Clinical Pharmacology and Toxicology, University of Lübeck Lübeck, Germany.
authors.forename: Godwin K
authors.initials: GK
authors.lastname: Dogbevia
authors.affiliation: NeuroCure Cluster of Excellence, Charité-Universitätsmedizin Berlin, Germany.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Marticorena-Alvarez
authors.affiliation: Department of Molecular Neurobiology, Max Planck Institute for Medical Research Heidelberg, Germany.
authors.forename: Melanie
authors.initials: M
authors.lastname: Bausen
authors.affiliation: Department of Molecular Neurobiology, Max Planck Institute for Medical Research Heidelberg, Germany.
authors.forename: Rolf
authors.initials: R
authors.lastname: Sprengel
authors.affiliation: Department of Molecular Neurobiology, Max Planck Institute for Medical Research Heidelberg, Germany ; NeuroCure Cluster of Excellence, Charité-Universitätsmedizin Berlin, Germany.
authors.forename: Mazahir T
authors.initials: MT
authors.lastname: Hasan
keywords: Cre mediated recombination; brain circuits; learning and plasticity; neurological diseases; rAAVs; tetracycline inducible systems