title: A case of tooth autotransplantation after long-term cryopreservation using a programmed freezer with a magnetic field.
abstract: This case report describes the treatment of a skeletal Class III malocclusion with autotransplantation of a cryopreserved tooth. To gain an esthetic facial profile and good occlusion, extraction of bimaxillary premolars and surgical therapy were chosen. The patient had chronic apical periodontitis on the lower left first molar. Although she did not feel any pain in that region, the tooth was considered to have a poor prognosis. Therefore, we cryopreserved the extracted premolars to prepare for autotransplantation in the lower first molar area because the tooth would probably need to be removed in the future. The teeth were frozen by a programmed freezer with a magnetic field (CAS freezer) that was developed for tissue cryopreservation and were cryopreserved in -150°C deep freezer. After 1.5 years of presurgical orthodontic treatment, bilateral sagittal split ramus osteotomy was performed for mandible setback. Improvement of the facial profile and the occlusion were achieved in the retention phase. Six years after the initial visit, the patient had pain on the lower left first molar, and discharge of pus was observed, so we extracted the lower left first molar and autotransplanted the cryopreserved premolar. Three years later, healthy periodontium was observed at the autotransplanted tooth. This case report suggests that long-term cryopreservation of teeth by a CAS freezer is useful for later autotransplantation, and this can be a viable technique to replace missing teeth.
authors.affiliation: a  Lecturer, Department of Orthodontics, Applied Life Sciences, Hiroshima University Institute of Biomedical & Health Sciences, Hiroshima, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Kaku
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shimasue
authors.forename: Junji
authors.initials: J
authors.lastname: Ohtani
authors.forename: Shunichi
authors.initials: S
authors.lastname: Kojima
authors.forename: Hiromi
authors.initials: H
authors.lastname: Sumi
authors.forename: Hanaka
authors.initials: H
authors.lastname: Shikata
authors.forename: Shotoku
authors.initials: S
authors.lastname: Kojima
authors.forename: Masahide
authors.initials: M
authors.lastname: Motokawa
authors.forename: Tahsin Raquib
authors.initials: TR
authors.lastname: Abonti
authors.forename: Toshitsugu
authors.initials: T
authors.lastname: Kawata
authors.forename: Kazuo
authors.initials: K
authors.lastname: Tanne
authors.forename: Kotaro
authors.initials: K
authors.lastname: Tanimoto
mesh_terms: D064592:Autografts / Q000637:transplantation*; D001641:Bicuspid / Q000637:transplantation*; D015925:Cryopreservation / Q000379:methods*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D055909:Magnetic Field Therapy / Q000379:methods; D008313:Malocclusion, Angle Class III / Q000601:surgery / Q000628:therapy; D008963:Molar / Q000601:surgery; D024343:Open Bite / Q000628:therapy; D019340:Osteotomy, Le Fort / Q000379:methods; D059229:Osteotomy, Sagittal Split Ramus / Q000379:methods; D010347:Patient Care Planning; D010485:Periapical Periodontitis / Q000601:surgery; D011842:Radicular Cyst / Q000601:surgery; D012390:Root Canal Therapy / Q000379:methods; D014081:Tooth Extraction / Q000379:methods; D020390:Tooth Socket / Q000601:surgery; D016896:Treatment Outcome; D055815:Young Adult
keywords: Autotransplantation; Cryopreservation; Magnetic field; Programmed freezer