title: Identification of post-transcriptional regulatory networks during myeloblast-to-monocyte differentiation transition.
abstract: Treatment of leukemia cells with 1,25-dihydroxyvitamin D3 may overcome their differentiation block and lead to the transition from myeloblasts to monocytes. To identify microRNA-mRNA networks relevant for myeloid differentiation, we profiled the expression of mRNAs and microRNAs associated to the low- and high-density ribosomal fractions in leukemic cells and in their differentiated monocytic counterpart. Intersection between mRNAs shifted across the fractions after treatment with putative target genes of modulated microRNAs showed a series of molecular networks relevant for the monocyte cell fate determination, as for example the post-transcriptional regulation of the Polo-like kinase 1 (PLK1) by miR-22-3p and let-7e-5p.
authors.affiliation: a Translational Oncogenomics Unit; "Regina Elena" National Cancer Institute ; Rome , Italy.
authors.forename: Giulia
authors.initials: G
authors.lastname: Fontemaggi
authors.forename: Teresa
authors.initials: T
authors.lastname: Bellissimo
authors.forename: Sara
authors.initials: S
authors.lastname: Donzelli
authors.forename: Ilaria
authors.initials: I
authors.lastname: Iosue
authors.forename: Barbara
authors.initials: B
authors.lastname: Benassi
authors.forename: Giorgio
authors.initials: G
authors.lastname: Bellotti
authors.forename: Giovanni
authors.initials: G
authors.lastname: Blandino
authors.forename: Francesco
authors.initials: F
authors.lastname: Fazi
mesh_terms: D018797:Cell Cycle Proteins / Q000378:metabolism; D002454:Cell Differentiation; D002762:Cholecalciferol / Q000378:metabolism; D053263:Gene Regulatory Networks; D042381:Granulocyte Precursor Cells / Q000166:cytology* / Q000378:metabolism; D018922:HL-60 Cells; D006801:Humans; D007938:Leukemia / Q000378:metabolism; D035683:MicroRNAs / Q000378:metabolism; D009000:Monocytes / Q000166:cytology* / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000378:metabolism*; D000097869:Polo-Like Kinase 1
chemical_list: D018797:Cell Cycle Proteins; D002762:Cholecalciferol; D035683:MicroRNAs; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D000097869:Polo-Like Kinase 1; C548375:MIRN22 microRNA, human; C487931:mirnlet7 microRNA, human
keywords: AGO2, argonaute 2; AML; AML, acute myeloid leukemia; ECL methods, enhanced chemiluminescence methods; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; GFP, green fluorescent protein; HPCs, haematopoietic progenitor cells; KPNA2, karyopherin α, 2; NBT assay, nitroblue tetrazolium assay; PLK1; PLK1, polo-like kinase 1; PMSF, phenylmethylsulfonyl fluoride; RAB10, member RAS oncogene family 10; RAB5C, member RAS oncogene family 5C; RT-qPCR, quantitative reverse transcription polymerase chain reaction; SF2A1, splicing factor 2A1; TFs, transcription factors; VitD3, 1,25-dihydroxyvitamin D3; miRNAs, microRNAs; microRNAs; myeloid differentiation; ribosomal/polysomal fractions