title: Transgenic carrot expressing fusion protein comprising M. tuberculosis antigens induces immune response in mice.
abstract: Tuberculosis remains one of the major infectious diseases, which continues to pose a major global health problem. Transgenic plants may serve as bioreactors to produce heterologous proteins including antibodies, antigens, and hormones. In the present study, a genetic construct has been designed that comprises the Mycobacterium tuberculosis genes cfp10, esat6 and dIFN gene, which encode deltaferon, a recombinant analog of the human γ-interferon designed for expression in plant tissues. This construct was transferred to the carrot (Daucus carota L.) genome by Agrobacterium-mediated transformation. This study demonstrates that the fusion protein CFP10-ESAT6-dIFN is synthesized in the transgenic carrot storage roots. The protein is able to induce both humoral and cell-mediated immune responses in laboratory animals (mice) when administered either orally or by injection. It should be emphasized that M. tuberculosis antigens contained in the fusion protein have no cytotoxic effect on peripheral blood mononuclear cells.
authors.affiliation: Institute of Cytology and Genetics, Russian Academy of Sciences, Prospect Lavrentieva 10, Novosibirsk 630090, Russia.
authors.forename: Natalia V
authors.initials: NV
authors.lastname: Permyakova
authors.affiliation: Institute of Cytology and Genetics, Russian Academy of Sciences, Prospect Lavrentieva 10, Novosibirsk 630090, Russia.
authors.forename: Alla A
authors.initials: AA
authors.lastname: Zagorskaya
authors.affiliation: Institute of Cytology and Genetics, Russian Academy of Sciences, Prospect Lavrentieva 10, Novosibirsk 630090, Russia.
authors.forename: Pavel A
authors.initials: PA
authors.lastname: Belavin
authors.affiliation: Institute of Cytology and Genetics, Russian Academy of Sciences, Prospect Lavrentieva 10, Novosibirsk 630090, Russia.
authors.forename: Elena A
authors.initials: EA
authors.lastname: Uvarova
authors.affiliation: Institute of Cytology and Genetics, Russian Academy of Sciences, Prospect Lavrentieva 10, Novosibirsk 630090, Russia ; State Research Center of Virology and Biotechnology Vector, Koltsovo, Novosibirsk 630559, Russia.
authors.forename: Olesya V
authors.initials: OV
authors.lastname: Nosareva
authors.affiliation: Institute of Cytology and Genetics, Russian Academy of Sciences, Prospect Lavrentieva 10, Novosibirsk 630090, Russia ; State Research Center of Virology and Biotechnology Vector, Koltsovo, Novosibirsk 630559, Russia.
authors.forename: Andrey E
authors.initials: AE
authors.lastname: Nesterov
authors.affiliation: Institute of Cytology and Genetics, Russian Academy of Sciences, Prospect Lavrentieva 10, Novosibirsk 630090, Russia.
authors.forename: Anna A
authors.initials: AA
authors.lastname: Novikovskaya
authors.affiliation: Institute of Cytology and Genetics, Russian Academy of Sciences, Prospect Lavrentieva 10, Novosibirsk 630090, Russia.
authors.forename: Evgeniy L
authors.initials: EL
authors.lastname: Zav'yalov
authors.affiliation: Institute of Cytology and Genetics, Russian Academy of Sciences, Prospect Lavrentieva 10, Novosibirsk 630090, Russia.
authors.forename: Mikhail P
authors.initials: MP
authors.lastname: Moshkin
authors.affiliation: Institute of Cytology and Genetics, Russian Academy of Sciences, Prospect Lavrentieva 10, Novosibirsk 630090, Russia.
authors.forename: Elena V
authors.initials: EV
authors.lastname: Deineko
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D018552:Daucus carota / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics; D051379:Mice; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D014376:Tuberculosis / Q000276:immunology / Q000382:microbiology
chemical_list: D000942:Antigens, Bacterial; D000949:Histocompatibility Antigens Class II; D011994:Recombinant Proteins; C072681:Mycobacterium tuberculosis antigens