title: Nur77 Was Essential for Neurite Outgrowth and Involved in Schwann Cell Differentiation After Sciatic Nerve Injury.
abstract: Nur77, together with Nurr1 and NOR-1, constitutes the NR4A subgroup of orphan nuclear receptors and plays critical roles in cell proliferation, differentiation, migration, and apoptosis. Among them, Nur77 is universally well known to contribute to neurite outgrowth. However, information regarding its regulation and possible function in the peripheral nervous system is still limited. In this study, we performed a sciatic nerve injury model in adult rats and detected an increased expression of Nur77 in the sciatic nerve, which was similar to the expression of Oct-6. Immunofluorescence indicated that Nur77 was located in both axons and Schwann cells. In vitro, we observed enhanced expression of Nur77 during the process of both basic fibroblast growth factor (bFGF)-induced Schwann cells differentiation and nerve growth factor (NGF)-induced PC12 cell neurite outgrowth. In vitro and in vivo experiments indicated that inhibiting the function of Nur77 by specific short hairpin RNA could depress Schwann cells myelinization and axons regeneration. Collectively, all these results suggested that upregulation of Nur77 might be involved in Schwann cells differentiation and neurite elongation following sciatic nerve crush.
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, People's Republic of China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Zhang
authors.forename: Xudong
authors.initials: X
authors.lastname: Zhu
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Minhao
authors.initials: M
authors.lastname: Chen
authors.forename: Shixian
authors.initials: S
authors.lastname: Yan
authors.forename: Xingxing
authors.initials: X
authors.lastname: Mao
authors.forename: Zhongbing
authors.initials: Z
authors.lastname: Liu
authors.forename: Weijie
authors.initials: W
authors.lastname: Wu
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.forename: Xinbao
authors.initials: X
authors.lastname: Xu
authors.forename: Youhua
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D008297:Male; D009186:Myelin Sheath / Q000378:metabolism; D009416:Nerve Regeneration; D016501:Neurites / Q000378:metabolism / Q000502:physiology; D055495:Neurogenesis; D057105:Nuclear Receptor Subfamily 4, Group A, Member 1 / Q000235:genetics / Q000378:metabolism*; D016716:PC12 Cells; D059348:Peripheral Nerve Injuries / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000166:cytology / Q000378:metabolism*; D012584:Sciatic Nerve / Q000378:metabolism / Q000473:pathology / Q000502:physiology*; D015854:Up-Regulation
chemical_list: C539820:Nr4a1 protein, rat; D057105:Nuclear Receptor Subfamily 4, Group A, Member 1