title: A quantitative imaging-based screen reveals the exocyst as a network hub connecting endocytosis and exocytosis.
abstract: The coupling of endocytosis and exocytosis underlies fundamental biological processes ranging from fertilization to neuronal activity and cellular polarity. However, the mechanisms governing the spatial organization of endocytosis and exocytosis require clarification. Using a quantitative imaging-based screen in budding yeast, we identified 89 mutants displaying defects in the localization of either one or both pathways. High-resolution single-vesicle tracking revealed that the endocytic and exocytic mutants she4∆ and bud6∆ alter post-Golgi vesicle dynamics in opposite ways. The endocytic and exocytic pathways display strong interdependence during polarity establishment while being more independent during polarity maintenance. Systems analysis identified the exocyst complex as a key network hub, rich in genetic interactions with endocytic and exocytic components. Exocyst mutants displayed altered endocytic and post-Golgi vesicle dynamics and interspersed endocytic and exocytic domains compared with control cells. These data are consistent with an important role for the exocyst in coordinating endocytosis and exocytosis.
authors.affiliation: Dynamics of Cell Growth and Division, European Institute of Chemistry and Biology, F-33607 Bordeaux, France Institut de Biochimie et Génétique Cellulaires, CNRS UMR 5095, Université Bordeaux, F-33000 Bordeaux, France Centre for Neuroscience, Indian Institute of Science, Bangalore 560012, India.
authors.forename: Mini
authors.initials: M
authors.lastname: Jose
authors.affiliation: Dynamics of Cell Growth and Division, European Institute of Chemistry and Biology, F-33607 Bordeaux, France Institut de Biochimie et Génétique Cellulaires, CNRS UMR 5095, Université Bordeaux, F-33000 Bordeaux, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Tollis
authors.affiliation: Centre for Neuroscience, Indian Institute of Science, Bangalore 560012, India Institut Interdisciplinaire de Neurosciences, CNRS UMR 5297, Université Bordeaux, F-33000 Bordeaux, France.
authors.forename: Deepak
authors.initials: D
authors.lastname: Nair
authors.affiliation: Dynamics of Cell Growth and Division, European Institute of Chemistry and Biology, F-33607 Bordeaux, France Institut de Biochimie et Génétique Cellulaires, CNRS UMR 5095, Université Bordeaux, F-33000 Bordeaux, France.
authors.forename: Romain
authors.initials: R
authors.lastname: Mitteau
authors.affiliation: Dynamics of Cell Growth and Division, European Institute of Chemistry and Biology, F-33607 Bordeaux, France Institut de Biochimie et Génétique Cellulaires, CNRS UMR 5095, Université Bordeaux, F-33000 Bordeaux, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Velours
authors.affiliation: Dynamics of Cell Growth and Division, European Institute of Chemistry and Biology, F-33607 Bordeaux, France Institut de Biochimie et Génétique Cellulaires, CNRS UMR 5095, Université Bordeaux, F-33000 Bordeaux, France.
authors.forename: Aurelie
authors.initials: A
authors.lastname: Massoni-Laporte
authors.affiliation: Institut de Biochimie et Génétique Cellulaires, CNRS UMR 5095, Université Bordeaux, F-33000 Bordeaux, France Institut de Biochimie et Génétique Cellulaires, CNRS UMR 5095, Université Bordeaux, F-33000 Bordeaux, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Royou
authors.affiliation: Institut Interdisciplinaire de Neurosciences, CNRS UMR 5297, Université Bordeaux, F-33000 Bordeaux, France.
authors.forename: Jean-Baptiste
authors.initials: JB
authors.lastname: Sibarita
authors.affiliation: Dynamics of Cell Growth and Division, European Institute of Chemistry and Biology, F-33607 Bordeaux, France Institut de Biochimie et Génétique Cellulaires, CNRS UMR 5095, Université Bordeaux, F-33000 Bordeaux, France mccusker@iecb.u-bordeaux.fr.
authors.forename: Derek
authors.initials: D
authors.lastname: McCusker
mesh_terms: D016764:Cell Polarity / Q000502:physiology; D004705:Endocytosis / Q000502:physiology*; D005089:Exocytosis / Q000502:physiology*; D053858:Metabolic Networks and Pathways; D021381:Protein Transport; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D004718:Saccharomycetales / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D029701:Saccharomyces cerevisiae Proteins