title: Bone marrow-derived mesenchymal stem cells attenuate phosgene-induced acute lung injury in rats.
abstract: Accidental phosgene exposure could result in acute lung injury (ALI), effective therapy is needed for the patients with phosgene-induced ALI. As a type of cells with therapeutic potential, mesenchymal stem cells (MSCs) have been showed its efficacy in multiple diseases. Here, we assessed the therapeutic potential of MSCs in phosgene-induced ALI and explored the related mechanisms. After isolation and characterization of rat bone marrow MSCs (BMMSCs), we transplanted BMMSCs into the rats exposed to phosgene and observed significant improvement on the lung wet-to-dry ratio and partial oxygen pressure (PaO2) at 6, 24, 48 h after phosgene exposure. Histological analyses revealed reduced sign of pathological changes in the lungs. Reduced level of pro-inflammatory tumor necrosis factor α and increased level of anti-inflammatory factor interleukin-10 were found in both bronchoalveolar lavage and plasma. Significant increased expression of epithelial cell marker AQP5 and SP-C was also found in the lung tissue. In conclusion, treatment with MSC markedly decreases the severity of phosgene-induced ALI in rats, and these protection effects were closely related to the pulmonary air blood barrier repairment and inflammatory reaction regulation.
authors.affiliation: Center of Emergency & Intensive Care Unit, Jinshan Hospital, Fudan University , Shanghai , China .
authors.forename: Junfeng
authors.initials: J
authors.lastname: Chen
authors.forename: Yiru
authors.initials: Y
authors.lastname: Shao
authors.forename: Guoxiong
authors.initials: G
authors.lastname: Xu
authors.forename: ChitChoon
authors.initials: C
authors.lastname: Lim
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Daojian
authors.initials: D
authors.lastname: Xu
authors.forename: Jie
authors.initials: J
authors.lastname: Shen
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D000818:Animals; D051402:Aquaporin 5 / Q000235:genetics; D001854:Bone Marrow Cells / Q000166:cytology; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D002619:Chemical Warfare Agents / Q000633:toxicity*; D016753:Interleukin-10 / Q000276:immunology; D008168:Lung / Q000276:immunology / Q000473:pathology; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D010455:Peptides / Q000235:genetics; D010705:Phosgene / Q000633:toxicity*; D012333:RNA, Messenger / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: C496040:Aqp5 protein, rat; D051402:Aquaporin 5; D002619:Chemical Warfare Agents; D010455:Peptides; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D010705:Phosgene; D016753:Interleukin-10; C077063:Sftpc protein, rat
keywords: Acute lung injury; mesenchymal stem cells; phosgene; therapy