title: Graphite-based microextraction by packed sorbent for online extraction of β-blockers from human plasma samples.
abstract: In the present work a new graphitic material (Carbon-XCOS) was used as a sorbent for microextraction by packed sorbent (MEPS). The β-blockers metoprolol and acebutolol in plasma samples were extracted and detected online using Carbon-MEPS syringe and liquid chromatography and tandem mass spectrometry (LC-MS/MS). Factors affecting the MEPS performance such as conditioning, washing and elution solutions were investigated. The validation of the bioanalytical method was performed using human plasma. The standard curve ranged from 10 to 2000nM and the lower limit of quantification (LLOQ) was set to 10nM. The method validation showed good accuracy and precision for the quality control (QC) samples at three concentration levels (30, 800 and 1600nM). The accuracy values of the QC samples were in the range of 86-108% (n=18). The precision values of intra- and inter-day for QC samples ranged from 4.4% to 14.4% (RSD) for the both studied analytes. The coefficient of determination (R(2)) values were ≥0.999 (n=3).
authors.affiliation: Department of Analytical Chemistry, Stockholm University, 106 91 Stockholm, Sweden.
authors.forename: Thana
authors.initials: T
authors.lastname: Abuzooda
authors.affiliation: Swedish Drug Agency,751 03 Uppsala, Sweden.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Amini
authors.affiliation: Department of Analytical Chemistry, Stockholm University, 106 91 Stockholm, Sweden. Electronic address: mohamed.abdel-rehim@aces.su.se.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Abdel-Rehim
mesh_terms: D000070:Acebutolol / Q000097:blood / Q000302:isolation & purification; D000319:Adrenergic beta-Antagonists / Q000097:blood* / Q000302:isolation & purification*; D002853:Chromatography, Liquid; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D008790:Metoprolol / Q000097:blood / Q000302:isolation & purification; D015203:Reproducibility of Results; D052617:Solid Phase Microextraction / Q000379:methods*
chemical_list: D000319:Adrenergic beta-Antagonists; D000070:Acebutolol; D006108:Graphite; D008790:Metoprolol
keywords: Graphitic sorbent; Microextraction by packed sorbent; Plasma samples; β-Blocker