title: Multiplex PCR-Based Serogrouping and Serotyping of Salmonella enterica from Tonsil and Jejunum with Jejunal Lymph Nodes of Slaughtered Swine in Metro Manila, Philippines.
abstract: Food poisoning outbreaks and livestock mortalities caused by Salmonella enterica are widespread in the Philippines, with hogs being the most commonly recognized carriers of the pathogen. To prevent and control the occurrence of S. enterica infection in the country, methods were used in this study to isolate and rapidly detect, differentiate, and characterize S. enterica in tonsils and jejuna with jejunal lymph nodes of swine slaughtered in four locally registered meat establishments (LRMEs) and four meat establishments accredited by the National Meat Inspection Services in Metro Manila. A total of 480 samples were collected from 240 animals (120 pigs from each type of meat establishment). A significantly higher proportion of pigs were positive for S. enterica in LRMEs (60 of 120) compared with meat establishments accredited by the National Meat Inspection Services (38 of 120). More S. enterica-positive samples were found in tonsils compared with jejuna with jejunal lymph nodes in LRMEs, but this difference was not significant. A PCR assay targeting the invA gene had sensitivity that was statistically similar to that of the culture method, detecting 93 of 98 culture-confirmed samples. Multiplex PCR-based O-serogrouping and H/Sdf I typing revealed four S. enterica serogroups (B, C1, D, and E) and six serotypes (Agona, Choleraesuis, Enteritidis, Heidelberg, Typhimurium, and Weltevreden), respectively, which was confirmed by DNA sequencing of the PCR products. This study was the first to report detection of S. enterica serotype Agona in the country.
authors.affiliation: Institute of Biology, College of Science, University of the Philippines, Diliman, Quezon City 1101, Philippines.
authors.forename: Kamela Charmaine S
authors.initials: KC
authors.lastname: Ng
authors.affiliation: Institute of Biology, College of Science, University of the Philippines, Diliman, Quezon City 1101, Philippines; Natural Sciences Research Institute, University of the Philippines, Diliman, Quezon City 1101, Philippines. wlrivera@science.upd.edu.ph.
authors.forename: Windell L
authors.initials: WL
authors.lastname: Rivera
mesh_terms: D000003:Abattoirs; D000818:Animals; D007583:Jejunum / Q000382:microbiology*; D008198:Lymph Nodes / Q000382:microbiology*; D008460:Meat / Q000382:microbiology; D008969:Molecular Sequence Data; D060885:Multiplex Polymerase Chain Reaction; D014066:Palatine Tonsil / Q000382:microbiology*; D010679:Philippines / Q000453:epidemiology; D010802:Phylogeny; D012481:Salmonella Infections, Animal / Q000453:epidemiology / Q000382:microbiology*; D019779:Salmonella enterica / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012703:Serotyping; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000382:microbiology*