title: Decreased expression of SOX17 is associated with tumor progression and poor prognosis in breast cancer.
abstract: The SOX17 (SRY-related HMG-box) transcription factor is involved in a variety of biological processes and is related to the tumorigenesis and progression of multiple tumors. However, the clinical application of SOX17 for breast cancer prognosis is currently limited. The aim of this study was to investigate the clinicopathologic and prognostic significance of SOX17 expression in human breast cancer. qPCR and western blot assays were performed to measure the expression of SOX17 in breast cancer cell lines and 30 matched pairs of breast cancer and corresponding noncancerous tissues. A SOX17 overexpression cell model was used to examine changes in cell growth in vitro. Immunohistochemical analyses were performed to retrospectively examine the prognostic impact of SOX17 expression in 187 additional breast cancer patients. Our results showed that SOX17 expression was decreased at both the messenger RNA (mRNA) and protein levels in the breast cancer cell lines and tissues, and that SOX17 overexpression could strongly suppress cell growth in vitro. Furthermore, the lack of SOX17 protein expression was strongly correlated with higher tumor grade (P = 0.002), lymph node metastasis (P < 0.001), and tumor node metastasis (TNM) stage (P = 0.001) and had poorer disease-free survival (DFS) and overall survival (OS) compared to normal SOX17 expression (P = 0.002 and 0.001, respectively). Univariate and multivariate analyses indicated that lower SOX17 expression was an independent prognostic factor for DFS (P = 0.007; HR = 2.854; 95 % CI 1.326-6.147) and OS (P = 0.005; HR = 5.035; 95 % CI 1.648-15.385) for breast cancer. Our findings indicate that SOX17 expression is a useful prognostic biomarker for breast cancer.
authors.affiliation: Department of Thyroid and Breast Surgery, Northern Jiangsu People's Hospital and Clinical Medical College of Yangzhou University, No. 98 Nantong West Road, Yangzhou, 225001, People's Republic of China. fdy1003@163.com.
authors.forename: De-Yuan
authors.initials: DY
authors.lastname: Fu
authors.affiliation: Department of Thyroid and Breast Surgery, Northern Jiangsu People's Hospital and Clinical Medical College of Yangzhou University, No. 98 Nantong West Road, Yangzhou, 225001, People's Republic of China.
authors.forename: Hao-Sheng
authors.initials: HS
authors.lastname: Tan
authors.affiliation: Department of Thyroid and Breast Surgery, Northern Jiangsu People's Hospital and Clinical Medical College of Yangzhou University, No. 98 Nantong West Road, Yangzhou, 225001, People's Republic of China.
authors.forename: Jin-Li
authors.initials: JL
authors.lastname: Wei
authors.affiliation: Department of Pathology, Northern Jiangsu People's Hospital and Clinical Medical College of Yangzhou University, Yangzhou, 225001, China.
authors.forename: Chang-Ren
authors.initials: CR
authors.lastname: Zhu
authors.affiliation: Department of Pathology, Northern Jiangsu People's Hospital and Clinical Medical College of Yangzhou University, Yangzhou, 225001, China.
authors.forename: Ji-Xin
authors.initials: JX
authors.lastname: Jiang
authors.affiliation: Department of Thyroid and Breast Surgery, Northern Jiangsu People's Hospital and Clinical Medical College of Yangzhou University, No. 98 Nantong West Road, Yangzhou, 225001, People's Republic of China.
authors.forename: Yu-Xiang
authors.initials: YX
authors.lastname: Zhu
authors.affiliation: Department of Thyroid and Breast Surgery, Northern Jiangsu People's Hospital and Clinical Medical College of Yangzhou University, No. 98 Nantong West Road, Yangzhou, 225001, People's Republic of China.
authors.forename: Feng-Lin
authors.initials: FL
authors.lastname: Cai
authors.affiliation: Department of Thyroid and Breast Surgery, Northern Jiangsu People's Hospital and Clinical Medical College of Yangzhou University, No. 98 Nantong West Road, Yangzhou, 225001, People's Republic of China.
authors.forename: Mei-Hong
authors.initials: MH
authors.lastname: Chong
authors.affiliation: The Clinical Medical Testing Laboratory, Northern Jiangsu People's Hospital and Clinical Medical College of Yangzhou University, Yangzhou, 225001, China.
authors.forename: Chuan-Li
authors.initials: CL
authors.lastname: Ren
mesh_terms: D000328:Adult; D000368:Aged; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D001940:Breast / Q000378:metabolism*; D001943:Breast Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D016022:Case-Control Studies; D049109:Cell Proliferation; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007124:Immunoenzyme Techniques; D008207:Lymphatic Metastasis; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055760:SOXF Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015996:Survival Rate; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger; C109188:SOX17 protein, human; D055760:SOXF Transcription Factors
keywords: Breast cancer; Prognosis; SOX17