title: A novel 1-Cys thioredoxin peroxidase gene in Apis cerana cerana: characterization of AccTpx4 and its role in oxidative stresses.
abstract: Thioredoxin peroxidase (Tpx), also named peroxiredoxin (Prx), is an important peroxidase that can protect organisms against stressful environments. AccTpx4, a 1-Cys thioredoxin peroxidase gene from the Chinese honey bee Apis cerana cerana, was cloned and characterized. The AccTpx4 gene encodes a protein that is predicted to contain the conserved PVCTTE motif from 1-Cys peroxiredoxin. Quantitative real-time PCR (Q-PCR) and Western blotting revealed that AccTpx4 was induced by various oxidative stresses, such as cold, heat, insecticides, H(2)O(2), and HgCl(2). The in vivo peroxidase activity assay showed that recombinant AccTpx4 protein could efficiently degrade H(2)O(2) in the presence of DL-dithiothreitol (DTT). In addition, disc fusion assays revealed that AccTpx4 could function to protect cells against oxidative stresses. These results indicate that AccTpx4 plays an important role in oxidative stress responses and may contribute to the conservation of honeybees.
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong, 271018, People's Republic of China.
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Huaxia
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Yan
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.forename: Hongfang
authors.initials: H
authors.lastname: Wang
authors.forename: Xingqi
authors.initials: X
authors.lastname: Guo
authors.forename: Baohua
authors.initials: B
authors.lastname: Xu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001516:Bees / Q000235:genetics*; D003001:Cloning, Molecular; D004229:Dithiothreitol / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000633:toxicity; D019476:Insect Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D007306:Insecticides / Q000633:toxicity; D008627:Mercuric Chloride / Q000633:toxicity; D008969:Molecular Sequence Data; D018384:Oxidative Stress / Q000187:drug effects; D054464:Peroxiredoxins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D060888:Real-Time Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D016415:Sequence Alignment; D013696:Temperature
chemical_list: D019476:Insect Proteins; D007306:Insecticides; D011994:Recombinant Proteins; D008627:Mercuric Chloride; D006861:Hydrogen Peroxide; D054464:Peroxiredoxins; D004229:Dithiothreitol