title: [The combined effect of E298D polymorphism of the endothelial nitric oxide synthase gene and smoking on the risk of cerebral stroke].
abstract: Violations of the endothelium-dependent regulation of cerebral vessel tone are an important link in the pathogenesis of cerebrovascular disorders. The purpose of this study was to investigate the association of--86T>C and E298D polymorphisms of the endothelial nitric oxide synthase(NOS3) gene with the risk of ce-ebral stroke (CS) in Russian inhabitants of Central Russia, as well as to evaluate the trigger effect of smoking on the risk of CS in carriers of genotypes NOS3. Genotyping of-786T>C and E298D polymorphisms of the NOS3 gene was carried out through real time. CR and TaqMan allele discrimination assays. It was deter-ined that the genotype 298DD is associated with the risk of CS (OR =-1.71, 95% CII= 1.05-2.78, P= 0.03). Subsequent analysis showed that genotype 298 DD (OR = 3.75; 95% CII= 1.39-10.11; P= 0.01) is associatedw ith an increased risk of CS exclusively in smoking individuals. The combination ofg enotypes -786T/Cx298D/D was associated with the risk of CS. n smokers (OR = 7.71; 95% CI = 1.31-45.34; P = 0.02). In the present study, it was found that smoking is a significant modifying risk factor for cerebral stroke in the carriers of the 298DD and -786T/C. enotypes of endothelial nitric oxide synthase.
authors.forename: O Yu
authors.initials: OY
authors.lastname: Bushueva
authors.forename: T A
authors.initials: TA
authors.lastname: Stetskaya
authors.forename: T V
authors.initials: TV
authors.lastname: Korogodina
authors.forename: V P
authors.initials: VP
authors.lastname: Ivanov
authors.forename: A V
authors.initials: AV
authors.lastname: Polonikov
mesh_terms: D000368:Aged; D000483:Alleles; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D012426:Russia; D012907:Smoking / Q000009:adverse effects* / Q000235:genetics; D020521:Stroke / Q000235:genetics* / Q000473:pathology; D044465:White People
chemical_list: C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III