title: An Adenoviral Vector Encoding Full-Length Dectin-1 Promotes Aspergillus-Induced Innate Immune Response in Macrophages.
abstract: BACKGROUND
  The incidence of invasive pulmonary aspergillosis (IPA) has increased significantly over the last two decades. Alveolar macrophages (AMs) represent the first line of pulmonary host response to Aspergillus conidia. Recognition of conidia by AMs involves Dectin-1 (CLEC7A), which is a conserved structure to combine β-glucans. The deficiency of Dectin-1 results in impaired fungal killing and uncontrolled growth of Aspergillus fumigatus. Thus, we hypothesized that high expression of Dectin-1 would enhance the host recognition and fungal killing.
  
  
  METHODS
  We set out to develop an adenoviral vector encoding full-length Dectin-1 (Ad-Dectin-1-EGFP) and then transfect it to MH-S cells. Transfect cell model was verified by using real-time RT-PCR, Western blot, flow cytometric, and confocal microscopic assays. And also, the function of Dectin-1 was explored by measuring cytokine release and killing ability during the course of A. fumigatus infection.
  
  
  RESULTS
  We constructed a recombinant adenovirus which could upregulate the expression of Dectin-1 and verified that Dectin-1 was expressed on cell membrane. The function of Dectin-1 was also demonstrated by its ability in promoting the production of cytokines and increasing the killing ability during the course of A. fumigatus infection.
  
  
  CONCLUSIONS
  An adenoviral vector was successfully applied to the production of a recombinant adenovirus encoding full-length Dectin-1, and also, its function in Aspergillus-induced innate immune response was demonstrated.
authors.affiliation: Department of Respiratory and Critical Care Medicine, Jinling Hospital, Medical School of Nanjing University, Nanjing, People's Republic of China.
authors.forename: Di
authors.initials: D
authors.lastname: Xia
authors.forename: Wen-Kui
authors.initials: WK
authors.lastname: Sun
authors.forename: Ming-Ming
authors.initials: MM
authors.lastname: Tan
authors.forename: Yuan
authors.initials: Y
authors.lastname: Ding
authors.forename: Zhi-Cheng
authors.initials: ZC
authors.lastname: Liu
authors.forename: Pei
authors.initials: P
authors.lastname: Li
authors.forename: Qian
authors.initials: Q
authors.lastname: Qian
authors.forename: Xin
authors.initials: X
authors.lastname: Su
authors.forename: Yi
authors.initials: Y
authors.lastname: Shi
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D001232:Aspergillus fumigatus / Q000276:immunology*; D015870:Gene Expression / Q000235:genetics; D005822:Genetic Vectors; D007113:Immunity, Innate / Q000235:genetics*; D016753:Interleukin-10 / Q000235:genetics; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D037181:Lectins, C-Type / Q000235:genetics*; D016676:Macrophages, Alveolar / Q000276:immunology*; D051379:Mice; D010587:Phagocytosis; D012333:RNA, Messenger / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics; D013172:Spores, Fungal / Q000276:immunology; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation; D000099181:Dectin-1
chemical_list: D016753:Interleukin-10; D053583:Interleukin-1beta; D037181:Lectins, C-Type; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D014409:Tumor Necrosis Factor-alpha; D000099181:Dectin-1