title: Differential Expression Patterns of Crystallin Genes during Ocular Development of Olive Flounder (Paralichthys olivaceus).
abstract: Olive flounder Paralichthys olivaceus is one of the most widely cultured fish species in Korea. Although olive flounder receive attention from aquaculture and fisheries and extensive research has been conducted eye morphological change in metamorphosis, but little information was known to molecular mechanism and gene expression of eye development- related genes during the early part of eye formation period. For the reason of eyesight is the most important sense in flounder larvae to search prey, the screening and identification of expressed genes in the eye will provide useful insight into the molecular regulation mechanism of eye development in olive flounder. Through the search of an olive flounder DNA database of expressed sequence tags (EST), we found a partial sequence that was similar to crystallin beta A1 and gamma S. Microscopic observation of retinal formation correspond with the time of expression of the crystallin beta A1 and gamma S gene in the developmental stage, these result suggesting that beta A1 and gamma S play a vital role in the remodeling of the retina during eye development. The expression of crystallin beta A1 and gamma S were obviously strong in eye at all tested developing stage, it is also hypothesized that crystallin acts as a molecular chaperone to prevent protein aggregation during maturation and aging in the eye.
authors.affiliation: Genetics and Breeding Research Center, NFRDI, Geoje 656-842, Korea.
authors.forename: Hyun
authors.initials: H
authors.lastname: Yang
authors.affiliation: Genetics and Breeding Research Center, NFRDI, Geoje 656-842, Korea.
authors.forename: Young Mee
authors.initials: YM
authors.lastname: Lee
authors.affiliation: Genetics and Breeding Research Center, NFRDI, Geoje 656-842, Korea.
authors.forename: Jae Koo
authors.initials: JK
authors.lastname: Noh
authors.affiliation: Genetics and Breeding Research Center, NFRDI, Geoje 656-842, Korea.
authors.forename: Hyun Chul
authors.initials: HC
authors.lastname: Kim
authors.affiliation: Genetics and Breeding Research Center, NFRDI, Geoje 656-842, Korea.
authors.forename: Choul Ji
authors.initials: CJ
authors.lastname: Park
authors.affiliation: Genetics and Breeding Research Center, NFRDI, Geoje 656-842, Korea.
authors.forename: Jong Won
authors.initials: JW
authors.lastname: Park
authors.affiliation: Genetics and Breeding Research Center, NFRDI, Geoje 656-842, Korea.
authors.forename: In Joon
authors.initials: IJ
authors.lastname: Hwang
authors.affiliation: Genetics and Breeding Research Center, NFRDI, Geoje 656-842, Korea.
authors.forename: Sung Yeon
authors.initials: SY
authors.lastname: Kim
authors.affiliation: Genetics and Breeding Research Center, NFRDI, Geoje 656-842, Korea.
authors.forename: Jeong Ho
authors.initials: JH
authors.lastname: Lee
keywords: Crystallin beta A1; Crystallin gamma S; Developmental stage; Eye; Gene expression; Olive flounder; Retina