title: Flagella but not type IV pili are involved in the initial adhesion of Pseudomonas aeruginosa PAO1 to hydrophobic or superhydrophobic surfaces.
abstract: Over the last decades, surface biocontamination has become a major concern in food industries and medical environments where its outcomes could vary from financial losses to public health issues. Understanding adhesion mechanisms of involved microorganisms is essential to develop new strategies of prevention and control. Adhesion of Pseudomonas aeruginosa, a nosocomial pathogenic bacterium, relies on several bacterial features, among which are bacterial appendages such as flagella and type IV pili. Here, we examine the role of P. aeruginosa PAO1 flagella and type IV pili in the adhesion to abiotic surfaces with various hydrophobicities. Adhesion kinetics showed, that after 60min, flagella increased the adhesion of the strain to surfaces with high hydrophobicity while no effect was observed on hydrophilic surfaces. Flagella of adherent bacteria exhibited specific and conserved pattern on the surfaces that suggested a higher affinity of flagella for hydrophobic surfaces. Based on these results and on previous studies in the literature, we proposed a model of flagella-mediated adhesion onto hydrophobic surfaces where these appendages induce the first contact and promote the adhesion of the bacterial body. These findings suggest that anti-bioadhesive surface design should take into consideration the presence of bacterial appendages.
authors.affiliation: INRA, AgroParisTech, UMR 1319 MICALIS, 78350 Jouy-en-Josas, France; AgroParisTech, INRA, UMR MICALIS, 91300 Massy, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Bruzaud
authors.affiliation: Université de Nice Sophia-Antipolis & CNRS, UMR 7336, 06108 Nice Cedex 2, France.
authors.forename: Jeanne
authors.initials: J
authors.lastname: Tarrade
authors.affiliation: CTTM, 72000 Le Mans, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Coudreuse
authors.affiliation: INRA, AgroParisTech, UMR 1319 MICALIS, 78350 Jouy-en-Josas, France; AgroParisTech, INRA, UMR MICALIS, 91300 Massy, France.
authors.forename: Alexis
authors.initials: A
authors.lastname: Canette
authors.affiliation: INRA, AgroParisTech, UMR 1319 MICALIS, 78350 Jouy-en-Josas, France; AgroParisTech, INRA, UMR MICALIS, 91300 Massy, France.
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Herry
authors.affiliation: Université de Nice Sophia-Antipolis & CNRS, UMR 7336, 06108 Nice Cedex 2, France.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Taffin de Givenchy
authors.affiliation: Université de Nice Sophia-Antipolis & CNRS, UMR 7336, 06108 Nice Cedex 2, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Darmanin
authors.affiliation: Université de Nice Sophia-Antipolis & CNRS, UMR 7336, 06108 Nice Cedex 2, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Guittard
authors.affiliation: INRA, AgroParisTech, UMR 1319 MICALIS, 78350 Jouy-en-Josas, France; AgroParisTech, INRA, UMR MICALIS, 91300 Massy, France.
authors.forename: Morgan
authors.initials: M
authors.lastname: Guilbaud
authors.affiliation: INRA, AgroParisTech, UMR 1319 MICALIS, 78350 Jouy-en-Josas, France; AgroParisTech, INRA, UMR MICALIS, 91300 Massy, France. Electronic address: marie-noelle.bellon-fontaine@agroparistech.fr.
authors.forename: Marie-Noëlle
authors.initials: MN
authors.lastname: Bellon-Fontaine
mesh_terms: D001422:Bacterial Adhesion / Q000502:physiology*; D003428:Cross Infection / Q000382:microbiology; D036781:Fimbriae Proteins / Q000737:chemistry* / Q000235:genetics; D005407:Flagella / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D009154:Mutation; D011093:Polyethylene Terephthalates / Q000737:chemistry; D011552:Pseudomonas Infections / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D013193:Stainless Steel / Q000737:chemistry; D013499:Surface Properties
chemical_list: D011093:Polyethylene Terephthalates; D013193:Stainless Steel; D036781:Fimbriae Proteins
keywords: Bacterial adhesion; Flagella; Physicochemical interactions; Pseudomonas aeruginosa; Surface structures; Type IV pili