title: Folding RNA-Protein Complex into Designed Nanostructures.
abstract: RNA-protein (RNP) complexes are promising biomaterials for the fields of nanotechnology and synthetic biology. Protein-responsive RNA sequences (RNP motifs) can be integrated into various RNAs, such as messenger RNA, short-hairpin RNA, and synthetic RNA nano-objects for a variety of purposes. Direct observation of RNP interaction in solution at high resolution is important in the design and construction of RNP-mediated nanostructures. Here we describe a method to construct and visualize RNP nanostructures that precisely arrange a target protein on the RNA scaffold with nanometer scale. High-speed AFM (HS-AFM) images of RNP nanostructures show that the folding of RNP complexes of defined sizes can be directly visualized at single RNP resolution in solution.
authors.affiliation: Center for iPS Cell Research and Application (CiRA), Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan.
authors.forename: Tomonori
authors.initials: T
authors.lastname: Shibata
authors.forename: Yuki
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sugiyama
authors.forename: Masayuki
authors.initials: M
authors.lastname: Endo
authors.forename: Hirohide
authors.initials: H
authors.lastname: Saito
mesh_terms: D024202:Electrophoretic Mobility Shift Assay / Q000379:methods; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D012313:RNA / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D012261:Ribonucleoproteins / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism
chemical_list: D012261:Ribonucleoproteins; D012313:RNA