title: Effects of dietary cellulase and xylanase addition on digestion, rumen fermentation and methane emission in growing goats.
abstract: The objective of this study was to evaluate the effectiveness of supplementation of cellulase and xylanase to diets of growing goats to improve nutrient digestibility, utilisation of energy and mitigation of enteric methane emissions. The experiment was conducted in a 5 × 5 Latin square design using five goats with permanent rumen fistulae and five treatments consisted of two levels of cellulase crossed over with two levels of xylanase plus unsupplemented Control. The cellulase (243 U/g) derived from Neocallimastix patriciarum was added at 0.8 and 1.6 g/kg dry matter intake (DMI) and the xylanase (31,457 U/ml) derived from Aspergillus oryzae was fed at 1.4 and 2.2 ml/kg DMI. There were no differences in apparent digestibility of organic matter, neutral detergent fibre, acid detergent fibre and rumen fermentation parameters (i.e. ammonia-nitrogen [N], volatile fatty acids) among all treatments. Dietary cellulase and xylanase addition did not influence energy and N utilisation. But compared to xylanase addition at the higher dose, at the low xylanase dose the retained N, the availability of retained N and digested N were increased (p < 0.01). Moreover, enzyme addition did not affect the enteric methane emission and community diversity of ruminal methanogens. The present results indicated that previous in vitro findings were not confirmed in ruminant trials.
authors.affiliation: a Key Laboratory for Agro-Ecological Processes in Subtropical Region, and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture , The Chinese Academy of Sciences , Changsha , Hunan , P.R. China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Lu
authors.forename: Jinzhen
authors.initials: J
authors.lastname: Jiao
authors.forename: Shaoxun
authors.initials: S
authors.lastname: Tang
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: He
authors.forename: Chuanshe
authors.initials: C
authors.lastname: Zhou
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Han
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Jinhe
authors.initials: J
authors.lastname: Kang
authors.forename: N E
authors.initials: NE
authors.lastname: Odongo
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Tan
mesh_terms: D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002480:Cellulase / Q000378:metabolism*; D004032:Diet / Q000662:veterinary; D004063:Digestion; D005260:Female; D005285:Fermentation; D006041:Goats / Q000254:growth & development / Q000382:microbiology / Q000502:physiology*; D008697:Methane / Q000378:metabolism; D012417:Rumen / Q000378:metabolism*; D014995:Xylosidases / Q000378:metabolism*
chemical_list: D014995:Xylosidases; D002480:Cellulase; D008697:Methane
keywords: cellulase; digestibility; feed additives; goats; methane production; rumen fermentation; xylanase