title: Integrin-β1, not integrin-β5, mediates osteoblastic differentiation and ECM formation promoted by mechanical tensile strain.
abstract: BACKGROUND
  Mechanical strain plays a great role in growth and differentiation of osteoblast. A previous study indicated that integrin-β (β1, β5) mediated osteoblast proliferation promoted by mechanical tensile strain. However, the involvement of integrin-β in osteoblastic differentiation and extracellular matrix (ECM) formation induced by mechanical tensile strain, remains unclear.
  
  
  RESULTS
  After transfection with integrin-β1 siRNA or integrin-β5 siRNA, mouse MC3T3-E1 preosteoblasts were cultured in cell culture dishes and stimulated with mechanical tensile strain of 2500 microstrain (με) at 0.5 Hz applied once a day for 1 h over 3 or 5 consecutive days. The cyclic tensile strain promoted osteoblastic differentiation of MC3T3-E1 cells. Transfection with integrin-β1 siRNA attenuated the osteoblastic diffenentiation induced by the tensile strain. By contrast, transfection with integrin-β5 siRNA had little effect on the osteoblastic differentiation induced by the strain. At the same time, the result of ECM formation promoted by the strain, was similar to the osteoblastic differentiation.
  
  
  CONCLUSIONS
  Integrin-β1 mediates osteoblast differentiation and osteoblastic ECM formation promoted by cyclic tensile strain, and integrin-β5 is not involved in the osteoblasts response to the tensile strain.
authors.affiliation: Shandong Provincial Key Laboratory of Functional Macromolecular Biophysics, Institute of Biophysics, Dezhou University, Dezhou, 253023, Shandong, China. qiangchengzeng@126.com.
authors.forename: Qiangcheng
authors.initials: Q
authors.lastname: Zeng
authors.affiliation: College of Biotechnology, Guilin Medical University, Guilin, 541004, Guangxi, China. guoyong74@163.com.|Institute of Medical Equipment, Academy of Military Medical Sciences, Tianjin, 300161, China. guoyong74@163.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Guo
authors.affiliation: College of Biotechnology, Guilin Medical University, Guilin, 541004, Guangxi, China. 1370536974@qq.com.
authors.forename: Yongming
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Medical Equipment, Academy of Military Medical Sciences, Tianjin, 300161, China. limxinxin@163.com.
authors.forename: Ruixin
authors.initials: R
authors.lastname: Li
authors.affiliation: Institute of Medical Equipment, Academy of Military Medical Sciences, Tianjin, 300161, China. xinchangzhang@163.com.
authors.forename: Xinchang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Chemistry Department, Logistics College of Chinese People's Armed Police Forces, Tianjin, China. netingcat@163.com.
authors.forename: Lu
authors.initials: L
authors.lastname: Liu
authors.affiliation: College of Biotechnology, Guilin Medical University, Guilin, 541004, Guangxi, China. 15294894750@163.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Medical Equipment, Academy of Military Medical Sciences, Tianjin, 300161, China. z56717@sohu.com.
authors.forename: Xizheng
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Biotechnology, Guilin Medical University, Guilin, 541004, Guangxi, China. xianqiongzou2009@yahoo.com.
authors.forename: Xianqiong
authors.initials: X
authors.lastname: Zou
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D049109:Cell Proliferation / Q000502:physiology; D005109:Extracellular Matrix / Q000502:physiology*; D039641:Integrin beta Chains / Q000502:physiology*; D019012:Integrin beta1 / Q000502:physiology*; D051379:Mice; D010006:Osteoblasts / Q000502:physiology*; D034741:RNA, Small Interfering; D060888:Real-Time Polymerase Chain Reaction; D013314:Stress, Mechanical; D013718:Tensile Strength / Q000502:physiology*; D014162:Transfection
chemical_list: D039641:Integrin beta Chains; D019012:Integrin beta1; D034741:RNA, Small Interfering; C084344:integrin beta5