title: [Mannan-Binding Lectin Inhibits Candida Albicans-Induced DC Maturation and Cytokine Secretion].
abstract: OBJECTIVE
  To investigate the effects of mannan-binding lectin (MBL) on the maturation and cytokine secretion of human dendritic cells (DC) induced by Candida albicans (C. albicans).
  
  
  METHODS
  The plastic-adherent mononuclear cells were prepared from the blood of healthy adult volunteers. The human peripheral blood mononuclear cells-derived dendritic cells (MNC-DC) were induced by 5-day-culture in medium supplemented with rhGM-CSF and rhIL-4, and then cultured for 2 days in presence or absence of C. albicans at varying concentration of human MBL ranging from 1 to 20 mg/L. DC's shape and characters were observed under inverted microscopy, the expression of CD83 and CD86 on DC was analyzed by FACS. The levels of TNF-α and IL-6 were detected by ELISA. FACS also was used to investigate the interaction of MBL with immature DC(imDC) and C. albicans. Western blot was used to detect C. albicans-induced IκBα phosphorylation and p65/NF-κB translocation in DC.
  
  
  RESULTS
  MBL at higher concentrations (10-20 mg/L) down-regulated the expression of CD83 and CD86 on the monocyte-derived dentritic cells(MoDC) induced by C. albicans, and inhibited the production of TNF-α and IL-6 induced by C. albicans. FACS showed that MBL could not only bind to C. albicans but also bind to imDCs in a Ca2+-dependent manner. Western blot showed that MBL could decrease the phosphorylation of IκBα and the nuclear translocation of p65/ NF-κB.
  
  
  CONCLUSIONS
  MBL may inhibit TNF-α and IL-6 production induced by C. albicans in DC through NF-κB signaling pathways, suggesting that MBL can play some roles in the regulation of C. albicans-induced immune response.
authors.affiliation: Sanquan Medical College, Xinxiang Medical University, Xinxiang 453003, Henan Province, China. E-mail: wmy118@126.com.|School of Laboratory Medicine, Xinxiang Medical University, Xinxiang 453003, Henan Province, China. E-mail: wmy118@126.com.
authors.forename: Ming-Yong
authors.initials: MY
authors.lastname: Wang
authors.affiliation: School of Laboratory Medicine, Xinxiang Medical University, Xinxiang 453003, Henan Province, China.
authors.forename: Fan-Ping
authors.initials: FP
authors.lastname: Wang
authors.affiliation: School of Laboratory Medicine, Xinxiang Medical University, Xinxiang 453003, Henan Province, China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Zhai
authors.affiliation: The Third Affiliated Hospital, Xinxiang Medical University, Xinxiang 453003, Henan Province, China.
authors.forename: Jun-Peng
authors.initials: JP
authors.lastname: Li
authors.affiliation: School of Laboratory Medicine, Xinxiang Medical University, Xinxiang 453003, Henan Province, China.
authors.forename: Na
authors.initials: N
authors.lastname: Zhang
authors.affiliation: School of Laboratory Medicine, Xinxiang Medical University, Xinxiang 453003, Henan Province, China.
authors.forename: Hao-Dian
authors.initials: HD
authors.lastname: Li
authors.affiliation: The Third Affiliated Hospital, Xinxiang Medical University, Xinxiang 453003, Henan Province, China.
authors.forename: Kang
authors.initials: K
authors.lastname: Guo
authors.affiliation: The Third Affiliated Hospital, Xinxiang Medical University, Xinxiang 453003, Henan Province, China.
authors.forename: Shi-Jun
authors.initials: SJ
authors.lastname: Song
authors.affiliation: School of Laboratory Medicine, Xinxiang Medical University, Xinxiang 453003, Henan Province, China.
authors.forename: Hai-Chuan
authors.initials: HC
authors.lastname: Yu
authors.affiliation: School of Laboratory Medicine, Xinxiang Medical University, Xinxiang 453003, Henan Province, China.
authors.forename: Wen-Xia
authors.initials: WX
authors.lastname: Zhao
authors.affiliation: School of Laboratory Medicine, Xinxiang Medical University, Xinxiang 453003, Henan Province, China.
authors.forename: Meng-Jie
authors.initials: MJ
authors.lastname: Li
mesh_terms: D002176:Candida albicans; D002454:Cell Differentiation; D016207:Cytokines; D003713:Dendritic Cells; D006801:Humans; D037601:Mannose-Binding Lectin; D016328:NF-kappa B; D021381:Protein Transport
chemical_list: D016207:Cytokines; D037601:Mannose-Binding Lectin; D016328:NF-kappa B