title: Relationship between CD4+CD25+ Treg and expression of HIF-1α and Ki-67 in NSCLC patients.
abstract: OBJECTIVE
  To investigate the relationship between the CD4+CD25+ Treg cell proportion in the peripheral blood and the clinicopathologic features of non-small cell lung cancer (NSCLC) patients and hypoxia-inducible factor-1α (HIF-1α), Ki-67 expression.
  
  
  METHODS
  Flow cytometry was used to measure the CD4+CD25+ Treg cell level in peripheral blood and immunohistochemical staining used to detect the expression of HIF-1α and Ki-67 protein in cancer tissue of each of 50 NSCLC patients.
  
  
  RESULTS
  The level of CD4+CD25+ Treg cell in peripheral blood was related to pathologic grades (t = 3.265, p = 0.006) and clinical stage (t = 4.417, p = 0. 001) of NSCLC instead of to patient's gender and pathologic type of tumor (p > 0.05). The level of CD4+CD25+ Treg cell was positively correlated with the expression of HIF-1α (r = 0.711, p = 0.003) and Ki-67 (r = 0.517, p = 0.04), respectively.
  
  
  CONCLUSIONS
  CD4+CD25+ Treg cell can be used as a predictor of immune status and prognosis of NSCLC patients and the levels of HIF-1α and Ki-67 protein expression may relate to inhibition of immune cells.
authors.affiliation: People's Hospital of Hebei Province, Shijiazhuang, Hebei Province, China. hongzhenzhang456@sohu.com.
authors.forename: L-Y
authors.initials: LY
authors.lastname: He
authors.forename: L
authors.initials: L
authors.lastname: Li
authors.forename: M-L
authors.initials: ML
authors.lastname: Guo
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: H-Z
authors.initials: HZ
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D002289:Carcinoma, Non-Small-Cell Lung / Q000175:diagnosis / Q000276:immunology / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000096:biosynthesis*; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology / Q000378:metabolism*; D019394:Ki-67 Antigen / Q000096:biosynthesis*; D008175:Lung Neoplasms / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; C508017:IL2RA protein, human; D053645:Interleukin-2 Receptor alpha Subunit; D019394:Ki-67 Antigen