title: Candida albicans erythroascorbate peroxidase regulates intracellular methylglyoxal and reactive oxygen species independently of D-erythroascorbic acid.
abstract: Candida albicans D-erythroascorbate peroxidase (EAPX1), which can catalyze the oxidation of D-erythroascorbic acid (EASC) to water, was observed to be inducible in EAPX1-deficient and EAPX1-overexpressing cells via activity staining. EAPX1-deficient cells have remarkably increased intracellular reactive oxygen species and methylglyoxal independent of the intracellular EASC content. The increased methylglyoxal caused EAPX1-deficient cells to activate catalase-peroxidase and cytochrome c peroxidase, which led to defects in cell growth, viability, mitochondrial respiration, filamentation and virulence. These findings indicate that EAPX1 mediates cell differentiation and virulence by regulating intracellular methylglyoxal along with oxidative stresses, regardless of endogenous EASC biosynthesis or alternative oxidase expression.
authors.affiliation: Laboratory of Biophysics, School of Biological Sciences, and Institute of Microbiology, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Min-Kyu
authors.initials: MK
authors.lastname: Kwak
authors.affiliation: Laboratory of Biophysics, School of Biological Sciences, and Institute of Microbiology, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Sung-Hyun
authors.initials: SH
authors.lastname: Song
authors.affiliation: Laboratory of Biophysics, School of Biological Sciences, and Institute of Microbiology, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: MyungHee
authors.initials: M
authors.lastname: Ku
authors.affiliation: Laboratory of Biophysics, School of Biological Sciences, and Institute of Microbiology, Seoul National University, Seoul 151-742, Republic of Korea. Electronic address: kangsaou@snu.ac.kr.
authors.forename: Sa-Ouk
authors.initials: SO
authors.lastname: Kang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060387:Ascorbate Peroxidases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001205:Ascorbic Acid / Q000378:metabolism*; D002176:Candida albicans / Q000201:enzymology* / Q000254:growth & development / Q000472:pathogenicity; D002454:Cell Differentiation; D005260:Female; D005978:Glutathione / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010101:Oxygen Consumption; D011765:Pyruvaldehyde / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014774:Virulence
chemical_list: D017382:Reactive Oxygen Species; C005056:isoascorbic acid; D011765:Pyruvaldehyde; D060387:Ascorbate Peroxidases; D005978:Glutathione; D001205:Ascorbic Acid
keywords: Candida albicans; Erythroascorbate peroxidase; Methylglyoxal; Reactive oxygen species; d-Erythroascorbic acid