title: Effect of Rhizobium and arbuscular mycorrhizal fungi inoculation on electrolyte leakage in Phaseolus vulgaris roots overexpressing RbohB.
abstract: Respiratory oxidative burst homolog (RBOH)-mediated reactive oxygen species (ROS) regulate a wide range of biological functions in plants. They play a critical role in the symbiosis between legumes and nitrogen-fixing bacteria or arbuscular mycorrhizal (AM) fungi. For instance, overexpression of PvRbohB enhances nodule numbers, but reduces mycorrhizal colonization in Phaseolus vulgaris hairy roots and downregulation has the opposite effect. In the present study, we assessed the effect of both rhizobia and AM fungi on electrolyte leakage in transgenic P. vulgaris roots overexpressing (OE) PvRbohB. We demonstrate that elevated levels of electrolyte leakage in uninoculated PvRbohB-OE transgenic roots were alleviated by either Rhizobium or AM fungi symbiosis, with the latter interaction having the greater effect. These results suggest that symbiont colonization reduces ROS elevated electrolyte leakage in P. vulgaris root cells.
authors.affiliation: a Departamento de Biología Molecular de Plantas ; Instituto de Biotecnología ; Universidad Nacional Autónoma de México (UNAM) ; Cuernavaca , México.
authors.forename: Manoj-Kumar
authors.initials: MK
authors.lastname: Arthikala
authors.forename: Noreide
authors.initials: N
authors.lastname: Nava
authors.forename: Carmen
authors.initials: C
authors.lastname: Quinto
mesh_terms: D015169:Colony Count, Microbial; D004573:Electrolytes / Q000378:metabolism*; D038821:Mycorrhizae / Q000502:physiology*; D027805:Phaseolus / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology*; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D018517:Plant Roots / Q000382:microbiology*; D030821:Plants, Genetically Modified; D017382:Reactive Oxygen Species / Q000378:metabolism; D012231:Rhizobium / Q000254:growth & development / Q000502:physiology*; D013559:Symbiosis
chemical_list: D004573:Electrolytes; D010940:Plant Proteins; D017382:Reactive Oxygen Species
keywords: P. vulgaris; RBOH; ROS; Rhizobium tropici; arbuscular mycorrhizal fungi (AMF); cell membrane stability; ion leakage; overexpression