title: Synthesis of a heterogeneous artificial metallolipase with chimeric catalytic activity.
abstract: A solid-phase strategy using lipase as a biomolecular scaffold to produce a large amount of Cu(2+)-metalloenzyme is proposed here. The application of this protocol on different 3D cavities of the enzyme allows creating a heterogeneous artificial metallolipase showing chimeric catalytic activity. The artificial catalyst was assessed in Diels-Alder cycloaddition reactions and cascade reactions showing excellent catalytic properties.
authors.affiliation: Departamento de Biocatálisis, Instituto de Catálisis, CSIC, Marie Curie 2, Campus UAM, 28049, Madrid, Spain. marco.filice1@gmail.com.
authors.forename: M
authors.initials: M
authors.lastname: Filice
authors.forename: O
authors.initials: O
authors.lastname: Romero
authors.forename: J
authors.initials: J
authors.lastname: Gutiérrez-Fernández
authors.forename: B
authors.initials: B
authors.lastname: de Las Rivas
authors.forename: J A
authors.initials: JA
authors.lastname: Hermoso
authors.forename: J M
authors.initials: JM
authors.lastname: Palomo
mesh_terms: D002384:Catalysis; D020134:Catalytic Domain; D008049:Lipase / Q000138:chemical synthesis* / Q000737:chemistry; D008667:Metalloproteins / Q000138:chemical synthesis* / Q000737:chemistry; D008958:Models, Molecular
chemical_list: D008667:Metalloproteins; D008049:Lipase