title: Phage display selections for affinity reagents to membrane proteins in nanodiscs.
abstract: Phage display selections generate high-affinity synthetic reagents that can be used as tools in structural characterization of membrane proteins. Currently, most selection protocols are performed with membrane protein targets in detergents. However, there are numerous technical issues associated with this, primarily that detergents are poor mimics of the native lipid environment. Here, we describe a set of protocols for phage display selection that involves reconstituting membrane proteins in nanodiscs, which are small discoidal particles consisting of lipids enclosed by membrane scaffold proteins. The nanodisc format enabled us to expand the capabilities of competitive and subtractive phage display selection steps, and generation of high-quality synthetic reagents for membrane proteins in native-like lipid environment.
authors.affiliation: Department of Biochemistry and Molecular Biology, Institute for Biophysical Dynamics, The University of Chicago, Chicago, Illinois, USA.
authors.forename: Pawel K
authors.initials: PK
authors.lastname: Dominik
authors.affiliation: Department of Biochemistry and Molecular Biology, Institute for Biophysical Dynamics, The University of Chicago, Chicago, Illinois, USA. Electronic address: koss@bsd.uchicago.edu.
authors.forename: Anthony A
authors.initials: AA
authors.lastname: Kossiakoff
mesh_terms: D000818:Animals; D019921:Biotinylation; D003902:Detergents / Q000737:chemistry; D006801:Humans; D007128:Immunoglobulin Fragments / Q000737:chemistry*; D007202:Indicators and Reagents; D008280:Magnetics / Q000379:methods; D059346:Magnets / Q000737:chemistry; D008563:Membrane Lipids / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry*; D008958:Models, Molecular; D049329:Nanostructures / Q000737:chemistry*; D019151:Peptide Library; D019809:Streptavidin / Q000737:chemistry
chemical_list: D003902:Detergents; D007128:Immunoglobulin Fragments; D007202:Indicators and Reagents; D008563:Membrane Lipids; D008565:Membrane Proteins; D019151:Peptide Library; D019809:Streptavidin
keywords: Competitive and subtractive selection; Crystallization chaperone; Membrane proteins; Nanodiscs; Phage display; Synthetic antibody fragment