title: Molecular diagnosis of strongyloidiasis in tropical areas: a comparison of conventional and real-time polymerase chain reaction with parasitological methods.
abstract: This study aimed to evaluate the use of conventional polymerase chain reaction (cPCR) and real-time quantitative PCR (qPCR) in the diagnosis of human strongyloidiasis from stool samples in tropical areas. Stool samples were collected from individuals and were determined to be positive for Strongyloides stercoralis (group I), negative for S. stercoralis (group II) and positive for other enteroparasite species (group III). DNA specific to S. stercoralis was found in 76.7% of group I samples by cPCR and in 90% of group I samples by qPCR. The results show that molecular methods can be used as alternative tools for detecting S. stercoralis in human stool samples in tropical areas.
authors.affiliation: Instituto de Medicina Tropical, Departamento de Moléstias Infecciosas e Parasitárias.
authors.forename: Fabiana Martins de
authors.initials: FM
authors.lastname: Paula
authors.affiliation: Instituto de Medicina Tropical, Departamento de Moléstias Infecciosas e Parasitárias.
authors.forename: Fernanda de Mello
authors.initials: Fde M
authors.lastname: Malta
authors.affiliation: Instituto de Medicina Tropical, Departamento de Moléstias Infecciosas e Parasitárias.
authors.forename: Priscilla Duarte
authors.initials: PD
authors.lastname: Marques
authors.affiliation: Instituto de Medicina Tropical, Departamento de Moléstias Infecciosas e Parasitárias.
authors.forename: Renata Barnabé
authors.initials: RB
authors.lastname: Sitta
authors.affiliation: Instituto de Medicina Tropical, Departamento de Moléstias Infecciosas e Parasitárias.
authors.forename: João Renato Rebello
authors.initials: JR
authors.lastname: Pinho
authors.affiliation: Instituto de Medicina Tropical, Departamento de Moléstias Infecciosas e Parasitárias.
authors.forename: Ronaldo César Borges
authors.initials: RC
authors.lastname: Gryschek
authors.affiliation: Instituto de Medicina Tropical, Departamento de Moléstias Infecciosas e Parasitárias.
authors.forename: Pedro Paulo
authors.initials: PP
authors.lastname: Chieffi
mesh_terms: D000818:Animals; D018509:DNA, Helminth / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D017171:Strongyloides stercoralis / Q000235:genetics / Q000302:isolation & purification*; D013322:Strongyloidiasis / Q000175:diagnosis*; D014329:Tropical Climate
chemical_list: D018509:DNA, Helminth