title: Estrogenicity and intersex in juvenile rainbow trout (Oncorhynchus mykiss) exposed to Pine/Eucalyptus pulp and paper production effluent in Chile.
abstract: Pulp and paper mill effluents (PPMEs) have been shown to increase gonad size, cause early maturation, and disrupt hormone functions in native and non-native Chilean fish. In this study, we assessed reproductive (plasma vitellogenin; VTG, gonad development) and metabolic (ethoxyresorufin-O-deethylase activity; EROD) end points, relative liver size (LSI) and condition factor (K) of juvenile female and male rainbow trout exposed to effluents. Unlike previous studies, which have focus either on the specific effects of effluent on fish in laboratory exposures or biotic population statuses downstream of discharge sites, we simultaneously assessed the impacts of PPMES on trout using two approaches: (1) laboratory exposures of tertiary treated PPME produced from processing Eucalyptus globulus or Pinus radiata; and (2) in situ bioassay downstream of the combined discharge of the same pulp mill. Despite an increase in the average gonadosomatic index (GSI) in exposed fish, no statistical differences in gonad size between exposed and unexposed individuals was detected. However, both female and male fish exposed to effluents showed significantly higher concentrations of plasma VTG, so more in fish exposed to Eucalyptus-based effluent when compared to Pinus PPME. In addition, male fish showed intersex characteristics in all exposure assays (Eucaliptus and Pinus) and, despite the low concentration of effluent in the river (<1% [v/v]), similar responses were observed in the caged fish. Finally, EROD activity was induced in both in situ exposures and laboratory assays at the higher PPME concentration (60-85% PPME). This study confirms estrogenic effects in Chilean fish exposed to PPME and the necessity for biological effects monitoring in addition to the assessment of physical-chemical endpoints as required in current government regulations.
authors.affiliation: Canadian Rivers Institute and Department of Biology, University of New Brunswick, Saint John, NB E2L 4L5, Canada. Electronic address: gchiang@centromeri.cl.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Chiang
authors.affiliation: Aquatic Systems Research Unit, EULA-Chile Environmental Sciences Centre, University of Concepción, Concepcion, Chile.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Barra
authors.affiliation: Laboratorio de Ecotoxicología y Contaminación Ambiental (ECoA), IIMyC-CONICET-UNMdP, Mar del Plata, Argentina.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Díaz-Jaramillo
authors.affiliation: Department of Zoology, Faculty of Natural and Oceanographic Sciences, University of Concepcion, Concepcion, Chile.
authors.forename: Meyling
authors.initials: M
authors.lastname: Rivas
authors.affiliation: Canadian Rivers Institute and Department of Biology, University of New Brunswick, Saint John, NB E2L 4L5, Canada.
authors.forename: Paulina
authors.initials: P
authors.lastname: Bahamonde
authors.affiliation: Canadian Rivers Institute and Department of Biology, University of New Brunswick, Saint John, NB E2L 4L5, Canada.
authors.forename: Kelly R
authors.initials: KR
authors.lastname: Munkittrick
mesh_terms: D000818:Animals; D002677:Chile; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D012734:Disorders of Sex Development / Q000139:chemically induced*; D004967:Estrogens; D005052:Eucalyptus / Q000737:chemistry*; D005260:Female; D006066:Gonads / Q000187:drug effects*; D007220:Industrial Waste; D008099:Liver / Q000187:drug effects; D008297:Male; D017686:Oncorhynchus mykiss / Q000502:physiology*; D010209:Paper; D028223:Pinus / Q000737:chemistry*; D012098:Reproduction / Q000187:drug effects; D045483:Rivers / Q000737:chemistry; D014819:Vitellogenins / Q000097:blood; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D004967:Estrogens; D007220:Industrial Waste; D014819:Vitellogenins; D014874:Water Pollutants, Chemical; D019363:Cytochrome P-450 CYP1A1
keywords: Bioassay; EROD; In situ; Intersex; Onchorhynchus mykiss; Pine and Eucalyptus pulp and paper mill effluents; Vitellogenin