title: Studying non-covalent drug-DNA interactions.
abstract: Drug-DNA interactions have been extensively studied in the recent past. Various techniques have been employed to decipher these interactions. DNA is a major target for a wide range of drugs that may specifically or non-specifically interact with DNA and affect its functions. Interaction between small molecules and DNA are of two types, covalent interactions and non-covalent interactions. Three major modes of non-covalent interactions are electrostatic interactions, groove binding and intercalative binding. This review primarily focuses on discussing various techniques used to study non-covalent interactions that occur between drugs and DNA. Additionally, we report several techniques that may be employed to analyse the binding mode of a drug with DNA. These techniques provide data that are reliable and simple to interpret.
authors.affiliation: Department of Biochemistry, Faculty of Life Sciences, A.M. University, Aligarh, U.P. 202002, India.
authors.forename: Sayeed Ur
authors.initials: SU
authors.lastname: Rehman
authors.affiliation: Department of Biochemistry, Faculty of Life Sciences, A.M. University, Aligarh, U.P. 202002, India.
authors.forename: Tarique
authors.initials: T
authors.lastname: Sarwar
authors.affiliation: Department of Biochemistry, Faculty of Life Sciences, A.M. University, Aligarh, U.P. 202002, India.
authors.forename: Mohammed Amir
authors.initials: MA
authors.lastname: Husain
authors.affiliation: Department of Biochemistry, Faculty of Life Sciences, A.M. University, Aligarh, U.P. 202002, India.
authors.forename: Hassan Mubarak
authors.initials: HM
authors.lastname: Ishqi
authors.affiliation: Department of Biochemistry, Faculty of Life Sciences, A.M. University, Aligarh, U.P. 202002, India. Electronic address: tabish.bcmlab@gmail.com.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Tabish
mesh_terms: D000818:Animals; D001665:Binding Sites; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007364:Intercalating Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D062105:Molecular Docking Simulation; D009690:Nucleic Acid Conformation; D004364:Pharmaceutical Preparations / Q000737:chemistry / Q000378:metabolism*; D055672:Static Electricity
chemical_list: D007364:Intercalating Agents; D004364:Pharmaceutical Preparations; D004247:DNA
keywords: Drug–DNA binding; Electrostatic interactions; Groove binding; Intercalation