title: Identification and functional characterization of the CSF1R gene from grass carp Ctenopharyngodon idellus and its use as a marker of monocytes/macrophages.
abstract: Colony-stimulating factor 1 receptor (CSF1R) is an important regulator of monocytes/macrophages (MO/MΦ). Although CSF1R gene has been identified and functionally studied in many fish, the precise role of CSF1R in grass carp (Ctenopharyngodon idellus) remains unclear. In this study, we determined the cDNA sequence of CSF1R (CiCSF1R) from a teleost fish, grass carp. Sequence comparison and phylogenetic tree analysis showed that CiCSF1R was most closely related to the CSF1R of zebrafish. The CiCSF1R transcript was mainly expressed in the spleen, head kidney, and head kidney-derived MO/MΦ, and its expression was altered in various tissues upon Aeromonas hydrophila infection. We prepared antibodies for neutralization of CiCSF1R on grass carp MO/MΦ. CiCSF1R neutralization or knockdown led to anti-inflammatory status in MO/MΦ upon A. hydrophila infection. CiCSF1R neutralization or knockdown also decreased the phagocytic activity of MO/MΦ. Flow cytometric analysis showed that more than 85% of grass carp MO/MΦ were CiCSF1R-positive cells. The percentage of CiCSF1R-positive cells in the head kidney of grass carp was above 10%, whereas it was only 5% and 4% in the spleen and liver, respectively. In conclusion, CSF1R is a specific surface marker of grass carp MO/MΦ, and it regulates the functions of MO/MΦ.
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo 315211, China; The Donghai Sea Collaborative Innovation Center for Industrial Upgrading Mariculture, Ningbo University, Ningbo 315211, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo 315211, China.
authors.forename: Xin-Jiang
authors.initials: XJ
authors.lastname: Lu
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo 315211, China; The Donghai Sea Collaborative Innovation Center for Industrial Upgrading Mariculture, Ningbo University, Ningbo 315211, China. Electronic address: jchen1975@163.com.
authors.forename: Jiong
authors.initials: J
authors.lastname: Chen
mesh_terms: D016980:Aeromonas hydrophila; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000276:immunology; D002347:Carps / Q000235:genetics / Q000276:immunology*; D016207:Cytokines / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli; D019143:Evolution, Molecular; D029941:Fish Proteins / Q000235:genetics / Q000276:immunology; D005880:Gills / Q000276:immunology; D016905:Gram-Negative Bacterial Infections / Q000276:immunology; D060226:Head Kidney / Q000276:immunology; D008099:Liver / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D008969:Molecular Sequence Data; D009000:Monocytes / Q000276:immunology*; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics / Q000276:immunology; D016415:Sequence Alignment; D013154:Spleen / Q000276:immunology
chemical_list: D016207:Cytokines; D018076:DNA, Complementary; D029941:Fish Proteins; D012333:RNA, Messenger; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor
keywords: Colony-stimulating factor 1 receptor; Flow cytometry; Inflammatory response; Monocytes/macrophages; Phagocytosis