title: miRNA-101 Suppresses Epithelial-to-Mesenchymal Transition by Targeting HMGA2 in Pancreatic Cancer Cells.
abstract: miR-101 is an outstanding tumor suppressor in various cancers, while its role in pancreatic cancer (PC) is still unknown. The aim of this study was to investigate the role of miR-101 in epithelial-to-mesenchymal transition (EMT) and its clinical relevance in PC. Our data showed that the miR-101 expression was significantly decreased in human PC tissues, compared to non-tumor counterparts (p<0.05), which was reversely correlated to clinical characteristics, including lymph node metastasis, more venous infiltration, higher expression of CA19-9 and TNM stage (p<0.05). Low miR-101 expression was also confirmed to be associated with a poorer overall survival rate in PC patients (p<0.05). We identified high-mobility group AT-hook 2 (HMGA2) gene as a putative target of miR-101 in PC by bioinformatics analysis, dual luciferase activity and western blot assay, and found that miR-101 could specifically target the HMGA2 3'-untranslated region (3'-UTR) (p<0.05). Knockdown of HMGA2 reversed EMT resembling that of miR-101 over-expression. An inverse correlation between miR-101 and HMGA2 was observed in patients with PC (p<0.05). Taken together, our findings speculated that miR-101 might act as an inhibiting factor in EMT process in PC and up-regulation of miR-101 might be considered as a potentially key molecular treatment strategy for PC patients.
authors.forename: Wenli
authors.initials: W
authors.lastname: Jiang
authors.forename: Wen
authors.initials: W
authors.lastname: Gu
authors.forename: Ronglin
authors.initials: R
authors.lastname: Qiu
authors.forename: Songbing
authors.initials: S
authors.lastname: He
authors.forename: Chenglong
authors.initials: C
authors.lastname: Shen
authors.forename: Yaohao
authors.initials: Y
authors.lastname: Wu
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Jiajia
authors.initials: J
authors.lastname: Zhou
authors.forename: Yixing
authors.initials: Y
authors.lastname: Guo
authors.forename: Daiwei
authors.initials: D
authors.lastname: Wan
authors.forename: Zhixi
authors.initials: Z
authors.lastname: Li
authors.forename: Jiemin
authors.initials: J
authors.lastname: Deng
authors.forename: Lexiang
authors.initials: L
authors.lastname: Zeng
authors.forename: Jin
authors.initials: J
authors.lastname: Tang
authors.forename: Jin
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, China, 215006. strexboy@163.com.
authors.forename: Qiaoming
authors.initials: Q
authors.lastname: Zhi
authors.forename: Xiaogeng
authors.initials: X
authors.lastname: Deng
mesh_terms: D049109:Cell Proliferation; D019295:Computational Biology; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D025743:HMGA2 Protein / Q000378:metabolism*; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000378:metabolism* / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D025743:HMGA2 Protein; C534072:MIRN101 microRNA, human; D035683:MicroRNAs