title: Intense Raman scattering on hybrid Au/Ag nanoplatforms for the distinction of MMP-9-digested collagen type-I fiber detection.
abstract: Well-ordered Au-nanorod arrays were fabricated using the focused ion beam method (denoted as fibAu_NR). Au or Ag nanoclusters (NCs) of various sizes and dimensions were then deposited on the fibAu_NR arrays using electron beam deposition to improve the surface-enhanced Raman scattering (SERS) effect, which was verified using a low concentration of crystal violet (10(-)(5)M) as the probe molecule. An enhancement factor of 6.92 × 10(8) was obtained for NCsfibAu_NR, which is attributed to the combination of intra-NC and NR localized surface plasmon resonance. When 4-aminobenzenethiol (4-ABT)-coated Au or Ag nanoparticles (NPs) were attached to NCsfibAu_NR, the small gaps between 4-ABT-coated NPs and intra-NCs allowed detection at the single-molecule level. Hotspots formed at the interfaces of NCs/NRs and NPs/NCs at a high density, producing a strong local electromagnetic effect. Raman spectra from as-prepared type I collagen (Col-I) and Ag-NP-coated Col-I fibers on NCsfibAu_NR were compared to determine the quantity of amino acids in their triple helix structure. Various concentrations of matrix-metalloproteinase-9-digested Col-I fibers on NCsfibAu_NR were qualitatively examined at a Raman laser wavelength of 785nm to determine the changes of amino acids in the Col-I fiber structure. The results can be used to monitor the growth of healing Col-I fibers in a micro-environment.
authors.affiliation: Department of Materials Science and Engineering, National Cheng Kung University, 1 University Road, Tainan 70101, Taiwan.
authors.forename: Kundan
authors.initials: K
authors.lastname: Sivashanmugan
authors.affiliation: Department of Materials Science and Engineering, National Cheng Kung University, 1 University Road, Tainan 70101, Taiwan; Center for Micro/Nano Science and Technology, National Cheng Kung University, 1 University Road, Tainan 70101, Taiwan. Electronic address: jdliao@mail.ncku.edu.tw.
authors.forename: Jiunn-Der
authors.initials: JD
authors.lastname: Liao
authors.affiliation: Department of Materials Science and Engineering, National Cheng Kung University, 1 University Road, Tainan 70101, Taiwan.
authors.forename: Pei-Lin
authors.initials: PL
authors.lastname: Shao
authors.affiliation: Department of Materials Science and Engineering, National Cheng Kung University, 1 University Road, Tainan 70101, Taiwan.
authors.forename: Bernard Haochih
authors.initials: BH
authors.lastname: Liu
authors.affiliation: Department of Materials Science and Engineering, National Cheng Kung University, 1 University Road, Tainan 70101, Taiwan.
authors.forename: Te-Yu
authors.initials: TY
authors.lastname: Tseng
authors.affiliation: Department of Materials Science and Engineering, National Cheng Kung University, 1 University Road, Tainan 70101, Taiwan.
authors.forename: Chih-Yu
authors.initials: CY
authors.lastname: Chang
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods; D024042:Collagen Type I / Q000032:analysis* / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D043942:Nanotubes / Q000737:chemistry / Q000648:ultrastructure; D051381:Rats; D012834:Silver / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013425:Sulfanilic Acids / Q000737:chemistry; D013499:Surface Properties
chemical_list: D024042:Collagen Type I; D013425:Sulfanilic Acids; D012834:Silver; C100016:4-sulfanilic acid; D006046:Gold; D020780:Matrix Metalloproteinase 9
keywords: 4-aminobenzenethiol; Au nanorods; Digested type I collagen; Nanoclusters; Surface-enhanced Raman scattering