title: Effect of Fimbristylis ovata on receptor for advanced glycation end-products, proinflammatory cytokines, and cell adhesion molecule level and gene expression in U937 and bEnd.3 cell lines.
abstract: Fimbristylis ovata has been long used as a traditional medicine for chronic inflammatory diseases; however, there are no data regarding its anti-inflammatory properties. In this study, we investigated the effects of F. ovata extracts on the secretion of pro-inflammatory cytokines, cell adhesion molecule, and receptor for advanced glycation end-products (RAGE) in lipopolysaccharide-stimulated cells. F. ovata was extracted using the maceration method with 3 different solvents: ethanol, methanol, and water. The effect of F. ovata extracts on cell viability was evaluated using the MTT assay. Pro-inflammatory cytokines and cell adhesion molecules were investigated by reverse transcription-polymerase chain reaction and an enzyme-linked immunosorbent assay. Upon incubation with F. ovata extracts up to 100 mg/mL, cell viability was more than 80%. F. ovata extracts could inhibit interleukin-6 level and gene expression as well as the RAGE gene in the monocytic cell lineU937. Moreover, the results showed that vascular cell adhesion molecule 1 secretion and gene expression were decreased when lipopolysaccharide-activated brain endothelial cells (bEnd.3) were treated with F. ovata extracts. Therefore, the anti-inflammatory activity of F. ovata extracts may result from their inhibitory actions via the RAGE signaling pathway.
authors.affiliation: Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand.
authors.forename: S
authors.initials: S
authors.lastname: Sukjamnong
authors.affiliation: Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand rachana.s@chula.ac.th.
authors.forename: R
authors.initials: R
authors.lastname: Santiyanont
mesh_terms: D000818:Animals; D015815:Cell Adhesion Molecules / Q000187:drug effects* / Q000235:genetics; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D029785:Cyperaceae / Q000737:chemistry*; D016207:Cytokines / Q000187:drug effects* / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics; D008070:Lipopolysaccharides / Q000378:metabolism; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D000067759:Receptor for Advanced Glycation End Products / Q000187:drug effects* / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics
chemical_list: D015815:Cell Adhesion Molecules; D016207:Cytokines; C508600:IL6 protein, human; D015850:Interleukin-6; D008070:Lipopolysaccharides; D010936:Plant Extracts; D000067759:Receptor for Advanced Glycation End Products; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1