title: Deciphering intracellular events triggered by mild magnetic hyperthermia in vitro and in vivo.
abstract: OBJECTIVE
  To assess the cell response to magnetic nanoparticles under an alternating magnetic field by molecular quantification of heat responsive transcripts in two model systems.
  
  
  METHODS
  Melanoma cells and Hydra vulgaris treated with magnetic nanoparticles were subjected to an alternating magnetic field or to macroscopic heating. Effect to these treatments were assessed at animal, cellular and molecular levels.
  
  
  RESULTS
  By comparing hsp70 expression following both treatments, thermotolerance pathways were found in both systems in absence of cell ablation or global temperature increment.
  
  
  CONCLUSIONS
  Analysis of hsp70 transcriptional activation can be used as molecular thermometer to sense cells' response to magnetic hyperthermia. Similar responses were found in cells and Hydra, suggesting a general mechanism to the delivery of sublethal thermal doses.
authors.affiliation: Instituto de Nanociencia de Aragon (INA), Universidad de Zaragoza, C/Mariano Esquillor s/n, 50018 Zaragoza, Spain.
authors.forename: Maria
authors.initials: M
authors.lastname: Moros
authors.affiliation: Istituto di Cibernetica "Eduardo Caianiello", Consiglio Nazionale delle Ricerche, Via Campi Flegrei, 34, 80078, Pozzuoli, Italy.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Ambrosone
authors.affiliation: Instituto de Nanociencia de Aragon (INA), Universidad de Zaragoza, C/Mariano Esquillor s/n, 50018 Zaragoza, Spain.
authors.forename: Grazyna
authors.initials: G
authors.lastname: Stepien
authors.affiliation: Istituto di Cibernetica "Eduardo Caianiello", Consiglio Nazionale delle Ricerche, Via Campi Flegrei, 34, 80078, Pozzuoli, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Fabozzi
authors.affiliation: Istituto di Cibernetica "Eduardo Caianiello", Consiglio Nazionale delle Ricerche, Via Campi Flegrei, 34, 80078, Pozzuoli, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Marchesano
authors.affiliation: Istituto di Cibernetica "Eduardo Caianiello", Consiglio Nazionale delle Ricerche, Via Campi Flegrei, 34, 80078, Pozzuoli, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Castaldi
authors.affiliation: Istituto di Cibernetica "Eduardo Caianiello", Consiglio Nazionale delle Ricerche, Via Campi Flegrei, 34, 80078, Pozzuoli, Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Tino
authors.affiliation: Instituto de Nanociencia de Aragon (INA), Universidad de Zaragoza, C/Mariano Esquillor s/n, 50018 Zaragoza, Spain.|Instituto de Ciencia de Materiales de Aragon, CSIC-Universidad de Zaragoza. C/Pedro Cerbuna 12, Zaragoza, Spain.
authors.forename: Jesus M
authors.initials: JM
authors.lastname: de la Fuente
authors.affiliation: Istituto di Cibernetica "Eduardo Caianiello", Consiglio Nazionale delle Ricerche, Via Campi Flegrei, 34, 80078, Pozzuoli, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Tortiglione
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000502:physiology; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006829:Hydra / Q000502:physiology; D006979:Hyperthermia, Induced / Q000379:methods*; D008280:Magnetics; D051379:Mice
chemical_list: D018840:HSP70 Heat-Shock Proteins
keywords: Hydra vulgaris; heat shock proteins; magnetic nanoparticles; murine melanoma cells; sublethal hyperthermia