title: Biochemical responses in mussels Perna perna exposed to diesel B5.
abstract: In Brazil B5 blend (5% biodiesel and 95% diesel oil) has been adopted as mandatory fuel since 2010 for automotive vehicles. Since little is known about the effects of B5 exposure can promote on antioxidant system of marine biota this study aimed to assess if B5 can generate modifications in antioxidant parameters of mussels Perna perna. To address this question mussels were exposed to two concentrations of B5 (0.01 mL L(-1) and 0.1 mL L(-1)) for 6h, 12h, 48 h and 168 h. Then the activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST) and glutathione reductase (GR) were evaluated in gills and digestive gland as well as the contents of glutathione (GSH) and lipid peroxidation by measuring the malondialdehyde concentration (MDA). In the gills, GST activity decreased after 48 h and GR after 12h of exposure to B5. In digestive glands, the activities of SOD, GPx and GR were changed due to treatments. GSH concentration increased in digestive gland after 6h and 12h and in gills after 48 h for B5 0.1 mL L(-1) and after 168 h in the digestive gland for B5 0.01 mL L(-1) treatment. No lipid peroxidation was detected. The integrated biomarker response index (IBR) evidenced a B5 effect in the digestive gland after 168 h of exposure. Regarding the experimental conditions and species used in this study, long-term exposure to B5 is apparently more likely to affect the parameters tested in P. perna mussels.
authors.affiliation: Laboratório de Biomarcadores de Contaminação Aquática, Departamento de Química e Ciências Ambientais, Universidade Estadual Paulista (IBILCE/UNESP), 15054-000 São José do Rio Preto, SP, Brazil. Electronic address: lilanog@gmail.com.
authors.forename: Lílian
authors.initials: L
authors.lastname: Nogueira
authors.affiliation: Laboratório de Biomarcadores de Contaminação Aquática, Departamento de Química e Ciências Ambientais, Universidade Estadual Paulista (IBILCE/UNESP), 15054-000 São José do Rio Preto, SP, Brazil. Electronic address: danielly005@hotmail.com.
authors.forename: Danielly
authors.initials: D
authors.lastname: Garcia
authors.affiliation: Laboratório de Defesas Celulares, Departamento de Bioquímica, Universidade Federal de Santa Catarina, 88040-900 Florianópolis, SC, Brazil. Electronic address: rafael.trevisan@gmail.com.
authors.forename: Rafael
authors.initials: R
authors.lastname: Trevisan
authors.affiliation: Laboratório de Biomarcadores de Contaminação Aquática, Departamento de Química e Ciências Ambientais, Universidade Estadual Paulista (IBILCE/UNESP), 15054-000 São José do Rio Preto, SP, Brazil. Electronic address: ana_leticia.88@hotmail.com.
authors.forename: Ana Letícia Madeira
authors.initials: AL
authors.lastname: Sanches
authors.affiliation: Laboratório de Defesas Celulares, Departamento de Bioquímica, Universidade Federal de Santa Catarina, 88040-900 Florianópolis, SC, Brazil. Electronic address: daiaacosta@yahoo.com.br.
authors.forename: Daiane
authors.initials: D
authors.lastname: da Silva Acosta
authors.affiliation: Laboratório de Defesas Celulares, Departamento de Bioquímica, Universidade Federal de Santa Catarina, 88040-900 Florianópolis, SC, Brazil. Electronic address: alcir.dafre@ufsc.br.
authors.forename: Alcir Luiz
authors.initials: AL
authors.lastname: Dafre
authors.affiliation: Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA. Electronic address: stratust@gmail.com.
authors.forename: Thiago Yukio Kikuchi
authors.initials: TY
authors.lastname: Oliveira
authors.affiliation: Laboratório de Biomarcadores de Contaminação Aquática, Departamento de Química e Ciências Ambientais, Universidade Estadual Paulista (IBILCE/UNESP), 15054-000 São José do Rio Preto, SP, Brazil. Electronic address: ealmeida@ibilce.unesp.br.
authors.forename: Eduardo Alves
authors.initials: EA
authors.lastname: de Almeida
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism; D056804:Biofuels; D015415:Biomarkers / Q000032:analysis; D001938:Brazil; D002374:Catalase / Q000378:metabolism; D005742:Gasoline; D005880:Gills / Q000187:drug effects* / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D049883:Perna / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D056804:Biofuels; D015415:Biomarkers; D005742:Gasoline; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D005978:Glutathione
keywords: B5; Biodiesel; Biomarker; Oxidative stress; Perna perna