title: Recruitment of IL-27-Producing CD4(+) T Cells and Effect of IL-27 on Pleural Mesothelial Cells in Tuberculous Pleurisy.
abstract: BACKGROUND
  The numbers of IL-27-producing CD4(+) T cells and the concentration of soluble IL-27 have been found to be increased in tuberculous pleural effusion (TPE). The objective of the present study was to explore the mechanism by which IL-27(+)CD4(+) T cells are recruited into the pleural space, and to explore the impact of IL-27 on pleural mesothelial cells (PMCs).
  
  
  METHODS
  The expression profiles of chemokine receptor (CCR) were determined by flow cytometry. The chemoattractant activity of chemokines CCL20 and CCL22 for IL-27(+)CD4(+) T cells in vitro was observed. Effects of IL-27 on wound healing, proliferation and apoptosis of PMCs were also investigated.
  
  
  RESULTS
  IL-27(+)CD4(+) T cells in TPE expressed high level of CCR6, medium level of CCR4, and low levels of CCR2, CCR3, CCR5, CCR7, CCR10, and CXCR3. Recruitment of IL-27(+)CD4(+) T cells into TPE could be induced by pleural CCL20 and CCL22. By activating STAT3 signaling, IL-27 significantly improved wound healing and promoted proliferation of PMCs, and completely prevented apoptosis of PMCs induced by IFN-γ.
  
  
  CONCLUSIONS
  After being recruited into pleural space by CCL20 or/and CCL22, these pleural IL-27-producing CD4(+) T cells may play important roles in tuberculosis immunity by affecting PMC functions.
authors.affiliation: Department of Respiratory Medicine, First People's Hospital of Foshan, Sun Yat-sen University, Foshan, China.
authors.forename: Zhi-Jian
authors.initials: ZJ
authors.lastname: Ye
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Xu
authors.forename: Qiong
authors.initials: Q
authors.lastname: Zhou
authors.forename: Ai
authors.initials: A
authors.lastname: Cui
authors.forename: Xiao-Juan
authors.initials: XJ
authors.lastname: Wang
authors.forename: Kan
authors.initials: K
authors.lastname: Zhai
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.forename: Zhao-Hui
authors.initials: ZH
authors.lastname: Tong
authors.forename: Huan-Zhong
authors.initials: HZ
authors.lastname: Shi
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D015496:CD4-Positive T-Lymphocytes / Q000737:chemistry / Q000187:drug effects / Q000276:immunology*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D054418:Chemokine CCL20 / Q000494:pharmacology; D054422:Chemokine CCL22 / Q000494:pharmacology; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D004847:Epithelial Cells / Q000187:drug effects*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D064094:Interleukin-27 / Q000032:analysis / Q000494:pharmacology*; D010994:Pleura / Q000166:cytology; D050796:STAT3 Transcription Factor / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D014396:Tuberculosis, Pleural / Q000276:immunology* / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects
chemical_list: D054418:Chemokine CCL20; D054422:Chemokine CCL22; D064094:Interleukin-27; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D007371:Interferon-gamma