title: DhITACT: DNA Hydrogel Formation by Isothermal Amplification of Complementary Target in Fluidic Channels.
abstract: DNA hydrogel formation by isothermal amplification of complementary targets in microfluidic channels (DhITACT) is a new platform for rapid and accurate detection of infectious pathogens. DNA hydrogel is formed in situ within microfluidic channels by the isothermal rolling circle amplification process upon the selective binding of target strands from the biological fluid. Once the volume of DNA hydrogel sufficiently enlarges, it can selectively block the matching channels with target pathogens.
authors.affiliation: College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul, 120-750, Republic of Korea.
authors.forename: Ho Yeon
authors.initials: HY
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul, 120-750, Republic of Korea.
authors.forename: Hansaem
authors.initials: H
authors.lastname: Jeong
authors.affiliation: College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul, 120-750, Republic of Korea.
authors.forename: Il Young
authors.initials: IY
authors.lastname: Jung
authors.affiliation: College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul, 120-750, Republic of Korea.
authors.forename: Bora
authors.initials: B
authors.lastname: Jang
authors.affiliation: Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 305-701, Republic of Korea.
authors.forename: Young Chang
authors.initials: YC
authors.lastname: Seo
authors.affiliation: Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 305-701, Republic of Korea.
authors.forename: Haeshin
authors.initials: H
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul, 120-750, Republic of Korea.
authors.forename: Hyukjin
authors.initials: H
authors.lastname: Lee
mesh_terms: D001408:Bacillus anthracis / Q000235:genetics / Q000302:isolation & purification; D004247:DNA / Q000737:chemistry* / Q000235:genetics*; D029043:Ebolavirus / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D056656:Lab-On-A-Chip Devices; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation*; D010641:Phenotype; D013696:Temperature
chemical_list: D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D004247:DNA
keywords: DNA hydrogel; microfluidic array chip; molecular diagnostic platform; rolling circle amplification