title: Protective Effect of Porcine Cerebral Hydrolysate Peptides on Learning and Memory Deficits and Oxidative Stress in Lead-Exposed Mice.
abstract: In this study, lead acetate solution and porcine cerebral hydrolysate peptides (PCHPs) were administered to developing mice. Porcine cerebral protein pretreated by ultrasound was hydrolyzed with alcalase, and 11 peptide fragments were obtained by Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis of PCHPs. Our data showed that PCHPs significantly decreased Pb2+-induced spontaneous locomotor activity, latencies to reach the platform, and the time in target quadrant. It also decreased the accumulation of lead in the blood and brain of Pb2+-exposed developing mice. Co-administration of PCHPs and dimercaptosuccinic acid (DMSA) did not only reduce the accumulation of lead in blood but also increased the absorption of zinc and iron in Pb2+-exposed mice. Administration of PCHPs individually significantly enhanced hematopoietic parameters compared with the Pb2+-exposed group. PCHPs significantly reduced the levels of reactive oxygen species (ROS) and malondialdehyde (MDA) but increased glutathione (GSH) content and anti-oxidant enzymes and nitric oxide synthase (NOS) activities in Pb2+-exposed brain. Our findings suggest that PCHPs have the ability to protect against Pb2+-exposed learning and memory deficits and oxidative damage.
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Rd., Zhenjiang, 212013, Jiangsu, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Zou
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Rd., Zhenjiang, 212013, Jiangsu, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Feng
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Rd., Zhenjiang, 212013, Jiangsu, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: School of the Environment, Jiangsu University, 301 Xuefu Rd., Zhenjiang, 212013, Jiangsu, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Chen
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, 301 Xuefu Rd., Zhenjiang, 212013, Jiangsu, China.
authors.forename: Zhaoxiang
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Rd., Zhenjiang, 212013, Jiangsu, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, 301 Xuefu Rd., Zhenjiang, 212013, Jiangsu, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhao
authors.affiliation: School of the Environment, Jiangsu University, 301 Xuefu Rd., Zhenjiang, 212013, Jiangsu, China.
authors.forename: Guanghua
authors.initials: G
authors.lastname: Mao
authors.affiliation: School of the Environment, Jiangsu University, 301 Xuefu Rd., Zhenjiang, 212013, Jiangsu, China. wuxy@ujs.edu.cn.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Wu
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, 301 Xuefu Rd., Zhenjiang, 212013, Jiangsu, China. yangliuqing@ujs.edu.cn.
authors.forename: Liuqing
authors.initials: L
authors.lastname: Yang
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D001921:Brain / Q000187:drug effects*; D006868:Hydrolysis; D007501:Iron / Q000097:blood; D007854:Lead / Q000009:adverse effects* / Q000097:blood; D007858:Learning; D018782:Maze Learning; D008569:Memory Disorders / Q000503:physiopathology*; D051379:Mice; D009068:Movement; D019001:Nitric Oxide Synthase / Q000737:chemistry; D009929:Organ Size; D018384:Oxidative Stress; D010455:Peptides / Q000737:chemistry*; D011492:Protein Hydrolysates / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013381:Subtilisins / Q000737:chemistry; D004113:Succimer / Q000737:chemistry; D013552:Swine; D014465:Ultrasonics; D015032:Zinc / Q000097:blood
chemical_list: D010455:Peptides; D011492:Protein Hydrolysates; D007854:Lead; D004113:Succimer; D007501:Iron; D019001:Nitric Oxide Synthase; D013381:Subtilisins; D015032:Zinc
keywords: Lead; Learning and memory deficits; NOS; Oxidative stress; Porcine cerebral hydrolysate peptides