title: Increased HDAC3 and decreased miRNA-130a expression in PBMCs through recruitment HDAC3 in patients with spinal cord injuries.
abstract: The study was performed to investigate the molecular mechanism for SCI patients. The interaction between miRNA-130a and HDAC was demonstrated in PBMCs from SCI patients. Increased HDAC3 and decreased miRNA-130a were observed in PBMCs from AS patients. Next, HDAC3 loss-of-function or HAAC3 inhibition promoted the expression of miRNA-130a, and HDAC3 could be recruited to the promoter region of the gene, miRNA-130a, in PBMCs. In addition, linear regression analysis indicated that mRNA expression results were highly negative correlated between HDAC3 and miRNA-130a in PBMCs from SCI patients. Furthermore, miRNA-130a down expression increased the expression of HDAC3 in PBMCs. Loss-of-function of miRNA-130a promoted PBMCs apoptosis, but HDAC3 loss-of-function had no significant effect on the apoptotic cell. In addition, miR-130a overexpression decreased, whereas miR-130a inhibition increased, the expression of TNF-α in PBMCs. Furthermore, HDAC3 loss-of-function or HAAC3 inhibition associated with simultaneous up-regulation the expression of miR-130a and down-regulation the expression of TNF-α in PBMCs. In conclusion, HDAC3 regulated a distinct underlying molecular and pathogenic mechanism of SCI by forming a negative feedback loop with miR-130a and enhanced TNF-1α expression.
authors.affiliation: Department of Orthopaedics, The Central Hospital of Taian 29 Longtan Road, Taian 271000, Shandong, China.
authors.forename: Yu-Dong
authors.initials: YD
authors.lastname: Ma
authors.affiliation: Department of Orthopaedics, The Central Hospital of Taian 29 Longtan Road, Taian 271000, Shandong, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Fang
authors.affiliation: Department of Orthopaedics, The Central Hospital of Taian 29 Longtan Road, Taian 271000, Shandong, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.affiliation: Office of Students, Affairs, Taishan Vocational Cellege of Nursing 8 Yingsheng Road, Taian 271000, Shandong, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
mesh_terms: D015153:Blotting, Western; D047369:Chromatin Immunoprecipitation; D005786:Gene Expression Regulation / Q000235:genetics*; D006655:Histone Deacetylases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D013119:Spinal Cord Injuries / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D000099186:Histone Deacetylase 3
chemical_list: D006655:Histone Deacetylases; D035683:MicroRNAs; D014409:Tumor Necrosis Factor-alpha; D000099186:Histone Deacetylase 3; C554019:MIRN130 microRNA, human
keywords: HDAC3; PBMCs; Spinal cord injury; miRNA-130a