title: Evaluation of a new rapid test for carbapenemase detection in carbapenem resistant Enterobacteriaceae.
abstract: We evaluated a new phenotypic test for carbapenemase detection. A total of 100 Enterobacteriaceae isolates were selected. The test was compared with conventional PCR for bla(KPC) and bla(NDM) detection. We found 100% sensitivity and specificity, suggesting that this test may be a feasible alternative for rapid carbapenemase detection.
authors.affiliation: Hospital Israelita Albert Einstein, São Paulo, Brazil; Faculdade de Ciências Médicas da Santa Casa de São Paulo, Brazil. Electronic address: marines.martino@einstein.br.
authors.forename: Marines Dalla Valle
authors.initials: MD
authors.lastname: Martino
authors.affiliation: Hospital Israelita Albert Einstein, São Paulo, Brazil.
authors.forename: Paula Célia Mariko
authors.initials: PC
authors.lastname: Koga
authors.affiliation: Hospital Israelita Albert Einstein, São Paulo, Brazil.
authors.forename: Jacyr
authors.initials: J
authors.lastname: Pasternak
authors.affiliation: Hospital Israelita Albert Einstein, São Paulo, Brazil.
authors.forename: André Mario
authors.initials: AM
authors.lastname: Doi
authors.affiliation: Hospital Beneficência Portuguesa de São Paulo, Brazil.
authors.forename: Claudete Silvia
authors.initials: CS
authors.lastname: Ciola
authors.affiliation: Irmandade da Santa Casa de Misericórdia de São Paulo, Brazil.
authors.forename: Cely Barreto
authors.initials: CB
authors.lastname: da Silva
authors.affiliation: Faculdade de Ciências Médicas da Santa Casa de São Paulo, Brazil.
authors.forename: Irineu Francisco Delfino Silva
authors.initials: IF
authors.lastname: Massaia
authors.affiliation: Hospital Israelita Albert Einstein, São Paulo, Brazil.
authors.forename: Itacy Gonçalves Siqueira
authors.initials: IG
authors.lastname: da Silva
authors.affiliation: Hospital Beneficência Portuguesa de São Paulo, Brazil.
authors.forename: Maria Rita Elmor
authors.initials: MR
authors.lastname: de Araújo
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D015780:Carbapenems / Q000494:pharmacology*; D004755:Enterobacteriaceae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000382:microbiology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D001618:beta-Lactamases; C063912:carbapenemase
keywords: Carbapenemases; Enterobacteriaceae; Phenotypic method; Resistance