title: Fast, Stable Induction of P-Glycoprotein-mediated Drug Resistance in BT-474 Breast Cancer Cells by Stable Transfection of ABCB1 Gene.
abstract: BACKGROUND
  Patients with P-glycoprotein and HER2/neu (HER2) receptor-overexpressing breast cancer usually have poor clinical outcomes. However, there exist no commercially available breast cancer cell lines that are HER2/P-glycoprotein double-positive, which limits research in this field.
  
  
  METHODS
  We report on the development and characterization of a drug-resistant sub-line from an HER2-positive breast cancer cell line by stable transfection of the ATP-binding cassette (ABC) subfamily B member 1 (ABCB1) gene which encodes P-glycoprotein.
  
  
  RESULTS
  ABCB1 gene expression levels were higher after transfection, which led to a 40-fold increase in P-glycoprotein expression. Interestingly, the transfection of ABCB1 also led to a slight increase in HER2 gene and protein expression levels. The transfection of ABCB1 increased the P-glycoprotein expression levels significantly.
  
  
  CONCLUSIONS
  The method used herein for developing this cell line is appropriate for fast, stable induction of P-glycoprotein-mediated drug resistance compared to traditional methods. The in vitro cytotoxicity test suggests this cell line has cross-resistance to a wide range of chemotherapeutic agents.
authors.affiliation: Department of Mechanical Engineering, Temple University, Philadelphia, PA, U.S.A.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Hematology and Medical Oncology, Lankenau Medical Center, Wynnewood, PA, U.S.A.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Mechanical Engineering, Temple University, Philadelphia, PA, U.S.A.
authors.forename: Sudhir
authors.initials: S
authors.lastname: Deosarkar
authors.affiliation: Department of Mechanical Engineering, Temple University, Philadelphia, PA, U.S.A.
authors.forename: Fariborz
authors.initials: F
authors.lastname: Soroush
authors.affiliation: Department of Mechanical Engineering, Temple University, Philadelphia, PA, U.S.A.
authors.forename: Mohammad F
authors.initials: MF
authors.lastname: Kiani
authors.affiliation: Department of Mechanical Engineering, Temple University, Philadelphia, PA, U.S.A. Department of Biomedical Engineering, Widener University, Chester, PA, U.S.A. bwang@wumail.widener.edu.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000096:biosynthesis / Q000235:genetics; D001943:Breast Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm / Q000187:drug effects / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis; D014162:Transfection
chemical_list: D018435:ATP Binding Cassette Transporter, Subfamily B; D018719:Erb-b2 Receptor Tyrosine Kinases; C513055:ABCB1 protein, human; C508053:ERBB2 protein, human
keywords: BT-474; HER2; MDR; P-glycoprotein; breast cancer; transfection