title: Role of a liver fatty acid-binding protein gene in lipid metabolism in chicken hepatocytes.
abstract: This study investigated the role of the chicken liver fatty acid-binding protein (L-FABP) gene in lipid metabolism in hepatocytes, and the regulatory relationships between L-FABP and genes related to lipid metabolism. The short hairpin RNA (shRNA) interference vector with L-FABP and an eukaryotic expression vector were used. Chicken hepatocytes were subjected to shRNA-mediated knockdown or L-FABP cDNA overexpression. Expression levels of lipid metabolism-related genes and biochemical parameters were detected 24, 36, 48, 60, and 72 h after transfection with the interference or overexpression plasmids for L-FABP, PPARα and L-BABP expression levels, and the total amount of cholesterol, were significantly affected by L-FABP expression. L-FABP may affect lipid metabolism by regulating PPARα and L-BABP in chicken hepatocytes.
authors.affiliation: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin, China.
authors.forename: G L
authors.initials: GL
authors.lastname: Gao
authors.affiliation: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin, China.
authors.forename: W
authors.initials: W
authors.lastname: Na
authors.affiliation: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin, China.
authors.forename: Y X
authors.initials: YX
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin, China.
authors.forename: H F
authors.initials: HF
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin, China.
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.affiliation: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin, China wangqigui@hotmail.com.
authors.forename: Q G
authors.initials: QG
authors.lastname: Wang
mesh_terms: D000818:Animals; D002645:Chickens / Q000235:genetics* / Q000378:metabolism; D050556:Fatty Acid-Binding Proteins / Q000037:antagonists & inhibitors / Q000235:genetics*; D005786:Gene Expression Regulation; D022781:Hepatocytes / Q000378:metabolism*; D050356:Lipid Metabolism / Q000235:genetics*; D047493:PPAR alpha / Q000235:genetics; D034741:RNA, Small Interfering
chemical_list: D050556:Fatty Acid-Binding Proteins; D047493:PPAR alpha; D034741:RNA, Small Interfering