title: The determination of DNA methyltransferase activity by quenching of tris(2,2'-bipyridine)ruthenium electrogenerated chemiluminescence with ferrocene.
abstract: An electrogenerated chemiluminescence (ECL) biosensing method for the determination of DNA methyltransferase activity is developed by the quenching of tris(2,2'-bipyridine)ruthenium ECL by ferrocene, and it is demonstrated that the ECL biosensing method measures DNA adenine methylation methyltransferase over a dynamic concentration range (0.1 U mL(-1)-100 U mL(-1)) with an extremely low detection limit of 0.03 U mL(-1), using gold nanoparticles and a quenching ECL signal produced by a chemical quencher such as ferrocene.
authors.affiliation: Key Laboratory of Electroanalytical Chemistry, Institute of Analytical Science, Northwest University, Xi'an, Shaanxi 710069, P. R. China. yanli@nwu.edu.cn.
authors.forename: Xiaoe
authors.initials: X
authors.lastname: Luo
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Jianbin
authors.initials: J
authors.lastname: Zheng
authors.forename: Honglan
authors.initials: H
authors.lastname: Qi
authors.forename: Zhenxing
authors.initials: Z
authors.lastname: Liang
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Ning
mesh_terms: D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives; D015374:Biosensing Techniques; D056831:Coordination Complexes; D015254:DNA Modification Methylases / Q000737:chemistry*; D055664:Electrochemical Techniques; D005296:Ferrous Compounds / Q000737:chemistry; D005472:Fluorouracil / Q000737:chemistry; D005839:Gentamicins / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D049449:Luminescence; D053768:Metal Nanoparticles / Q000737:chemistry; D000075163:Metallocenes; D017975:Ruthenium Compounds
chemical_list: D015082:2,2'-Dipyridyl; D056831:Coordination Complexes; D015254:DNA Modification Methylases; D005296:Ferrous Compounds; D005472:Fluorouracil; D005839:Gentamicins; D006046:Gold; D000075163:Metallocenes; C547232:tris(2,2-bipyridine)-ruthenium(II); D017975:Ruthenium Compounds; C004998:ferrocene