title: [Effect of non-classical secreted proteins on LipaseA secretion].
abstract: OBJECTIVE
  We used 50-amino acid-long peptides from the N-terminus of 4 different non-classically secreted proteins to study the secretion efficiency of Bacillus subtilis LipaseA via non-classical secretion pathway.
  
  
  METHODS
  We amplified the coding sequences (CDs) of LipaseA and N-terminus of non-classically secreted proteins, constructed 8 fusion protein expression vectors containing both LipaseA CD and different secretion signal peptide and transformed them into B. subtilis WB800. Secretion efficiency of these fusion proteins was analyzed by enzyme activity, SDS-PAGE and Western-Blot.
  
  
  RESULTS
  Recombinant LipaseA containing coding sequences of PdhA or N-terminus of SodA and Eno as secretion signals was efficiently secreted.
  
  
  CONCLUSIONS
  Parts of non-classically secreted proteins or N-terminus (50 amino acids) could guide LipaseA protein secretion.
authors.forename: Jing
authors.initials: J
authors.lastname: Cui
authors.forename: Guangqiang
authors.initials: G
authors.lastname: Wang
authors.forename: Haiqin
authors.initials: H
authors.lastname: Chen
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Zhennan
authors.initials: Z
authors.lastname: Gu
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D058947:Bacterial Secretion Systems; D008049:Lipase / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport
chemical_list: D001426:Bacterial Proteins; D058947:Bacterial Secretion Systems; D008049:Lipase