title: Graphene-CNT nanohybrid aptasensor for label free detection of cardiac biomarker myoglobin.
abstract: We report a label free electrochemical detection of cardiac bio-marker myoglobin (Mb) on aptamer functionalized rGO/CNT nanostructured electrodes by measuring its direct electron transfer (DET). Configured as a highly responsive aptasensor, the newly developed biosensing platform exhibits synergistic effect of the nano-hybrid functional construct by combining good electrical properties and the facile chemical functionality of nanohybrid for the compatible bio-interface development. The specific anti-Mb aptamer was generated by five iterative SELEX (Systematic evolution of ligands by exponential enrichment) rounds, showing high senstivity (KD ~65 pM). The aptamer functionalized rGO/CNT nanostructured electrodes demonstrated a significant increase in signal response with a detection limit of ~0.34 ng/mL in the dynamic response range between 1 ng/mL and 4 µg/mL for Mb. The newly developed DET assay format presents a promising candidate in point-of-care diagnosis for routine screening of Mb in patient's samples.
authors.affiliation: Institute of Nano Science & Technology, Mohali 160062, India.
authors.forename: Vinod
authors.initials: V
authors.lastname: Kumar
authors.affiliation: Institute of Nano Science & Technology, Mohali 160062, India.
authors.forename: Munish
authors.initials: M
authors.lastname: Shorie
authors.affiliation: Institute of Nano Science & Technology, Mohali 160062, India. Electronic address: ashok@inst.ac.in.
authors.forename: Ashok K
authors.initials: AK
authors.lastname: Ganguli
authors.affiliation: Institute of Nano Science & Technology, Mohali 160062, India. Electronic address: priyanka@inst.ac.in.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Sabherwal
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015415:Biomarkers / Q000032:analysis; D015374:Biosensing Techniques / Q000295:instrumentation; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004566:Electrodes; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D008958:Models, Molecular; D009211:Myoglobin / Q000032:analysis*; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D019095:Point-of-Care Systems; D052156:SELEX Aptamer Technique
chemical_list: D052157:Aptamers, Nucleotide; D015415:Biomarkers; D009211:Myoglobin; D037742:Nanotubes, Carbon; D006108:Graphite
keywords: Aptamer; Cardiac marker; Direct electron transfer; Myoglobin; rGO/CNT nanohybrid