title: Novel Detection of Insecticide Resistance Related P450 Genes and Transcriptome Analysis of the Hemimetabolous Pest Erthesina fullo (Thunberg) (Hemiptera: Heteroptera).
abstract: Erthesina fullo (Thunberg, 1783) is an economically important heteropteran species in China. Since only three nucleotide sequences of this species (COI, 16S rRNA, and 18S rRNA) appear in the GenBank database so far, no analysis of the molecular mechanisms underlying E. fullo's resistance to insecticide and environmental stress has been accomplished. We reported a de novo assembled and annotated transcriptome for adult E. fullo using the Illumina sequence system. A total of 53,359,458 clean reads of 4.8 billion nucleotides (nt) were assembled into 27,488 unigenes with an average length of 750 bp, of which 17,743 (64.55%) were annotated. In the present study, we identified 88 putative cytochrome P450 sequences and analyzed the evolution of cytochrome P450 superfamilies, genes of the CYP3 clan related to metabolizing xenobiotics and plant natural compounds, in E. fullo, increasing the candidate genes for the molecular mechanisms of insecticide resistance in P450. The sequenced transcriptome greatly expands the available genomic information and could allow a better understanding of the mechanisms of insecticide resistance at the systems biology level.
authors.affiliation: Institute of Entomology, College of Life Sciences, Nankai University, Tianjin, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Entomology, College of Life Sciences, Nankai University, Tianjin, China.
authors.forename: Haoyang
authors.initials: H
authors.lastname: Wu
authors.affiliation: Institute of Entomology, College of Life Sciences, Nankai University, Tianjin, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xie
authors.affiliation: Institute of Entomology, College of Life Sciences, Nankai University, Tianjin, China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Bu
mesh_terms: D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D020869:Gene Expression Profiling; D049750:Genome, Insect; D020062:Heteroptera / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing; D007305:Insecticide Resistance / Q000235:genetics*; D058977:Molecular Sequence Annotation
chemical_list: D003577:Cytochrome P-450 Enzyme System