title: Detection and Genome Analysis of a Lineage III Peste Des Petits Ruminants Virus in Kenya in 2011.
abstract: In May 2011 in Turkana County, north-western Kenya, tissue samples were collected from goats suspected of having died of peste des petits ruminant (PPR) disease, an acute viral disease of small ruminants. The samples were processed and tested by reverse transcriptase PCR for the presence of PPR viral RNA. The positive samples were sequenced and identified as belonging to peste des petits ruminants virus (PPRV) lineage III. Full-genome analysis of one of the positive samples revealed that the virus causing disease in Kenya in 2011 was 95.7% identical to the full genome of a virus isolated in Uganda in 2012 and that a segment of the viral fusion gene was 100% identical to that of a virus circulating in Tanzania in 2013. These data strongly indicate transboundary movement of lineage III viruses between Eastern Africa countries and have significant implications for surveillance and control of this important disease as it moves southwards in Africa.
authors.affiliation: Animal Production and Heath Laboratory (APHL), Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Vienna, Austria.
authors.forename: W G
authors.initials: WG
authors.lastname: Dundon
authors.affiliation: Department of Veterinary Pathology, Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Nairobi, Uthiru, Kenya.
authors.forename: S M
authors.initials: SM
authors.lastname: Kihu
authors.affiliation: Department of Veterinary Pathology, Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Nairobi, Uthiru, Kenya.
authors.forename: G C
authors.initials: GC
authors.lastname: Gitao
authors.affiliation: Department of Veterinary Pathology, Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Nairobi, Uthiru, Kenya.
authors.forename: L C
authors.initials: LC
authors.lastname: Bebora
authors.affiliation: Department of Veterinary Pathology, Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Nairobi, Uthiru, Kenya.
authors.forename: N M
authors.initials: NM
authors.lastname: John
authors.affiliation: Institute of Tropical and Infectious Diseases (UNITID), University of Nairobi, Nairobi, Kenya.
authors.forename: J O
authors.initials: JO
authors.lastname: Oyugi
authors.affiliation: Institute for Veterinary Disease Control, Austrian Agency for Health and Food Safety, Moedling, Austria.
authors.forename: A
authors.initials: A
authors.lastname: Loitsch
authors.affiliation: Animal Production and Heath Laboratory (APHL), Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Vienna, Austria.
authors.forename: A
authors.initials: A
authors.lastname: Diallo
mesh_terms: D000818:Animals; D016679:Genome, Viral; D006041:Goats; D007630:Kenya; D018107:Peste-des-petits-ruminants virus / Q000235:genetics*; D010802:Phylogeny; D012367:RNA, Viral / Q000302:isolation & purification
chemical_list: D012367:RNA, Viral
keywords: Kenya; genome; lineage III; peste des petits ruminants; phylogeny