title: [miR-328 Inhibits K562 Cell Proliferation by Up-regulation of C/EBPα Expression].
abstract: OBJECTIVE
  Our research was aim to investigate the effect of microRNA-328 (miR-328) on proliferation of chronic myeloid leukemia(CML) cell K562 and the mediated effect of C/EBPα.
  
  
  METHODS
  The eukaryotic expression vectors of miR-328 targeting gene and suppressor gene (hsa-miR-328 and hsa-miR-328-inhibitor) were constructed, and transfected into K562 cells respectively. The mRNA expression levels of miR-328 and C/EBP α were detected by real-time fluorescence quantitative RT-PCR; C/EBP α protein expression was detected by Western blot; CCK-8 was used to estimate the cell viability.
  
  
  RESULTS
  The recombinant genes of hsa-miR-328 and hsa-miR-328-inhibitor were successfully constructed and transfected into K562 cells. Fluorescent cells were observed after 24 h, and the visible fluorescence cells were gradually increased after 48 h or 72 h, the miR-328 showed no effect on the mRNA expression of C/EBPα detected by RT-PCR. Meanwhile, miR-328 showed recovering effect on C/EBPα translation and inhibition of K562 cells proliferation.
  
  
  CONCLUSIONS
  miR-328 has been successfully constructed and transfected into K562 cells, miR-328 inhibits the proliferation of K562 cells by up-regulation of C/EBPα.
authors.affiliation: Department of Hematology, The First Hospital, Nanhua University, Hengyang 421000, Hunan Province, China.
authors.forename: Jin-Xia
authors.initials: JX
authors.lastname: Cao
authors.affiliation: Department of Hematology, The First Hospital, Nanhua University, Hengyang 421000, Hunan Province, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wen
authors.affiliation: Department of Neurology, The First Hospital, Nanhua University, Hengyang 421000, Hunan Province, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Hematology, The First Hospital, Nanhua University, Hengyang 421000, Hunan Province, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Liao
authors.affiliation: Department of Hematology, The First Hospital, Nanhua University, Hengyang 421000, Hunan Province, China. E-mail: hylijunjun@163.com.
authors.forename: Jun-Jun
authors.initials: JJ
authors.lastname: Li
mesh_terms: D022763:CCAAT-Enhancer-Binding Protein-alpha; D049109:Cell Proliferation; D002470:Cell Survival; D006801:Humans; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive; D035683:MicroRNAs; D014162:Transfection; D015854:Up-Regulation
chemical_list: D022763:CCAAT-Enhancer-Binding Protein-alpha; C548170:MIRN328 microRNA, human; D035683:MicroRNAs