title: Down-regulation of Immune-related Genes by PSCA in Gallbladder Cancer Cells Implanted into Mice.
abstract: OBJECTIVE
  In previous work, we found that prostate stem cell antigen (PSCA) gene, encoding a glycosylphosphatidylinositol-anchored protein, is a presumable tumor suppressor in gastric cancer and gallbladder cancer (GBC). The introduction of PSCA cDNA into GBC cell lines significantly suppressed tumorigenecity of cells in mice. The PSCA protein is thought to be involved in some form of intracellular signaling that remains to be elucidated.
  
  
  METHODS
  Using microarrays, we conducted gene-expression profiling on tumors generated by a GBC cell line TGBC-1TKB, with and without expression of PSCA, which was implanted into mice. Genes whose expression was down-regulated by PSCA were selected, and their down-regulation was confirmed by real-time PCR.
  
  
  RESULTS
  We identified several immune-related genes down-regulated by PSCA, including interleukin 8 (IL8), IL1 receptor antagonist (IL1RN) and S100 calcium-binding proteins A8 (S100A8) and A9 (S100A9).
  
  
  CONCLUSIONS
  PSCA signaling may suppress tumor growth in vivo by modulating immunological characteristics of GBC cells.
authors.affiliation: Division of Genetics, National Cancer Center Research Institute, Chuo-ku, Tokyo, Japan nsaeki@ncc.go.jp.
authors.forename: Norihisa
authors.initials: N
authors.lastname: Saeki
authors.affiliation: Division of Genetics, National Cancer Center Research Institute, Chuo-ku, Tokyo, Japan.
authors.forename: Hiroe
authors.initials: H
authors.lastname: Ono
authors.affiliation: Division of Genetics, National Cancer Center Research Institute, Chuo-ku, Tokyo, Japan.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Sakamoto
authors.affiliation: Division of Genetics, National Cancer Center Research Institute, Chuo-ku, Tokyo, Japan.
authors.forename: Teruhiko
authors.initials: T
authors.lastname: Yoshida
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D040501:Calgranulin A / Q000096:biosynthesis / Q000276:immunology; D040502:Calgranulin B / Q000096:biosynthesis / Q000276:immunology; D045744:Cell Line, Tumor; D058851:GPI-Linked Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D005706:Gallbladder Neoplasms / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D017261:Glycosylphosphatidylinositols / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein / Q000096:biosynthesis / Q000276:immunology; D016209:Interleukin-8 / Q000096:biosynthesis / Q000276:immunology; D051379:Mice; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology
chemical_list: D000951:Antigens, Neoplasm; D040501:Calgranulin A; D040502:Calgranulin B; D058851:GPI-Linked Proteins; D017261:Glycosylphosphatidylinositols; C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D016209:Interleukin-8; D009363:Neoplasm Proteins; C110843:PSCA protein, human
keywords: GPI-anchored protein; Prostate stem cell antigen; gallbladder cancer; intracellular signaling; tumor suppressor