title: Protective effect of high concentration of BN52021 on retinal contusion in cat eyes.
abstract: BACKGROUND
  Blunt injuries/contusion on eyes might cause retina blunt trauma. This study is to evaluate the protective function of BN52021 against retinal trauma.
  
  
  METHODS
  A total of 70 cats, 6 months old, were divided into six groups: Group A to E (n = 12) and normal control (N) group (n = 10). The right eyes in Group A to E were contused. All experiments were performed under general anesthetization. Retrobulbar injections of medication in right eyes were performed. Cats were administrated with 0.5 mL of normal saline (NS), dimethyl sulphoxide, 0.2 g/L BN52021, 1 g/L BN52021 and 5 g/L BN52021, respectively. Cats in Group N were administrated with 0.5 mL of NS. Intraocular pressure (IOP), flash electroretinogram (ERG), and retinal nerve fiber layer (RNFL) thickness were measured. Hematoxylin and eosin (HE) staining and transmission electron microscope (TEM) were detected.
  
  
  RESULTS
  No significant difference was observed in IOP levels among groups. Comparing with cats in Group N, those in Group A to E showed significant lower amplitudes of rod a- and b-waves (P < 0.05). Amplitudes of rod a- and b-waves were increased by administration of high concentration of BN52021 (≥ 1 g/L). Moreover, high concentration of BN52021 decreased the RNFL thickness increased by contusion. Axons in RNFL in Group E arranged neatly at 7 days after modeling.
  
  
  CONCLUSIONS
  The degenerated axons caused by contusion were repaired by BN52021. The administration of high concentration of (≥ 1 g/L) BN52021 could partially repair retinal function in contused cat eyes.
authors.affiliation: Department of Ophthalmology, 307 Hospital, PLA, Beijing, 100071, China. jinfenghuangjfh@163.com.
authors.forename: Jin-Feng
authors.initials: JF
authors.lastname: Huang
authors.affiliation: Department of Ophthalmology, CHINA-JAPAN Friendship Hospital, No.2 Yinghua Dongjie, Hepingli, Beijing, 100029, China. haipengzhaohpz@163.com.
authors.forename: Hai-Peng
authors.initials: HP
authors.lastname: Zhao
authors.affiliation: Department of Ophthalmology, 307 Hospital, PLA, Beijing, 100071, China. yanfengyangyfy@163.com.
authors.forename: Yan-Feng
authors.initials: YF
authors.lastname: Yang
authors.affiliation: Department of Ophthalmology, 307 Hospital, PLA, Beijing, 100071, China. huiminhuanghmh@163.com.
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Huang
authors.affiliation: Department of Ophthalmology, The General Hospital of PLA, No. 28 Fuxing Road, Beijing, 100853, China. yiyaodoubley@163.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Department of Ophthalmology, CHINA-JAPAN Friendship Hospital, No.2 Yinghua Dongjie, Hepingli, Beijing, 100029, China. zhijunwangzjw@163.com.
authors.forename: Zhi-Jun
authors.initials: ZJ
authors.lastname: Wang
mesh_terms: D000818:Animals; D002415:Cats; D003288:Contusions / Q000175:diagnosis / Q000188:drug therapy*; D004195:Disease Models, Animal; D004596:Electroretinography / Q000187:drug effects; D005131:Eye Injuries / Q000175:diagnosis / Q000188:drug therapy*; D005260:Female; D005343:Fibrinolytic Agents / Q000008:administration & dosage*; D046934:Ginkgolides / Q000008:administration & dosage*; D007429:Intraocular Pressure; D007783:Lactones / Q000008:administration & dosage*; D008297:Male; D009412:Nerve Fibers / Q000187:drug effects / Q000473:pathology; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors; D012160:Retina / Q000293:injuries*; D012164:Retinal Diseases / Q000175:diagnosis / Q000188:drug therapy*; D012165:Retinal Ganglion Cells / Q000187:drug effects / Q000473:pathology; D041623:Tomography, Optical Coherence; D014065:Tonometry, Ocular
chemical_list: D005343:Fibrinolytic Agents; D046934:Ginkgolides; D007783:Lactones; D010972:Platelet Activating Factor; C045856:ginkgolide B