title: TORC2 and eisosomes are spatially interdependent, requiring optimal level of phosphatidylinositol 4, 5-bisphosphate for their integrity.
abstract: The elucidation of the organization and maintenance of the plasma membrane has been sought due to its numerous roles in cellular function. In the budding yeast Saccharomyces cerevisiae, a novel paradigm has begun to emerge in the understanding of the distribution of plasma membrane microdomains and how they are regulated. We aimed to investigate the dynamic interdependence between the protein complexes eisosome and TORC2, representing microdomains MCC and MCT, respectively. In this study, we reveal that the eisosome organizer Pil1 colocalizes with the MCT marker Avo2. Furthermore, we provide evidence that the formation of MCT is dependent on both eisosome integrity and adequate levels of the plasma membrane phosphoinositide PI(4,5)P2. Taken together, our findings indicate that TORC2, eisosomes, and PI(4,5)P2 exist in an interconnected relationship, which supports the emerging model of the plasma membrane.
authors.affiliation: Department of Biology, Missouri State University, 901 S National Ave, Springfield, MO 65807, USA.
authors.forename: Katelyn
authors.initials: K
authors.lastname: Bartlett
authors.forename: Shiva Kumar Goud
authors.initials: SK
authors.lastname: Gadila
authors.forename: Brandon
authors.initials: B
authors.lastname: Tenay
authors.forename: Hyoeun
authors.initials: H
authors.lastname: McDERMOTT
authors.forename: Brett
authors.initials: B
authors.lastname: Alcox
authors.forename: Kyoungtae
authors.initials: K
authors.lastname: Kim
mesh_terms: D002352:Carrier Proteins / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology; D000076225:Mechanistic Target of Rapamycin Complex 2; D021962:Membrane Microdomains / Q000378:metabolism*; D046912:Multiprotein Complexes / Q000378:metabolism*; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism*
chemical_list: C503707:Avo2 protein, S cerevisiae; D002352:Carrier Proteins; D046912:Multiprotein Complexes; C487327:PIL1 protein, S cerevisiae; D019269:Phosphatidylinositol 4,5-Diphosphate; D010750:Phosphoproteins; D029701:Saccharomyces cerevisiae Proteins; D003609:Dactinomycin; D000076225:Mechanistic Target of Rapamycin Complex 2; D058570:TOR Serine-Threonine Kinases