title: TM7 detection in human microbiome: Are PCR primers and FISH probes specific enough?
abstract: TM7 appears important and omnipresent because it is repeatedly detected by molecular techniques in diverse environments. Here we report that most of primers and FISH probes thought to be TM7-specific do hybridize with multiple species from oral and vaginal cavity. This calls for re-examination of TM7 distribution and abundance.
authors.affiliation: Department of Biology, Northeastern University, Boston, MA 02115, USA.
authors.forename: Maria V
authors.initials: MV
authors.lastname: Sizova
authors.affiliation: Department of Biology, Northeastern University, Boston, MA 02115, USA.
authors.forename: Sebastian N
authors.initials: SN
authors.lastname: Doerfert
authors.affiliation: Department of Biology, Northeastern University, Boston, MA 02115, USA.
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Gavrish
authors.affiliation: Department of Biology, Northeastern University, Boston, MA 02115, USA. Electronic address: s.epstein@neu.edu.
authors.forename: Slava S
authors.initials: SS
authors.lastname: Epstein
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D064307:Microbiota; D009055:Mouth / Q000382:microbiology; D015345:Oligonucleotide Probes / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014621:Vagina / Q000382:microbiology
chemical_list: D017931:DNA Primers; D015345:Oligonucleotide Probes
keywords: Microbial cultivation; Oral microbiome; TM7