title: Analysis of changes in microRNA expression profiles in response to the troxerutin-mediated antioxidant effect in human dermal papilla cells.
abstract: Dermal papilla (DP) cells function as important regulators of the hair growth cycle. The loss of these cells is a primary cause of diseases characterized by hair loss, including alopecia, and evidence has revealed significantly increased levels of reactive oxygen species (ROS) in hair tissue and DP cells in the balding population. In the present study, troxerutin, a flavonoid derivative of rutin, was demonstrated to have a protective effect against H2O2-mediated cellular damage in human DP (HDP) cells. Biochemical assays revealed that pretreatment with troxerutin exerted a protective effect against H2O2-induced loss of cell viability and H2O2-induced cell death. Further experiments confirmed that troxerutin inhibited the H2O2-induced production of ROS and upregulation of senescence-associated β-galactosidase activity. Using microRNA (miRNA) microarrays, the present study identified 24 miRNAs, which were differentially expressed in the troxerutin-pretreated, H2O2-treated HDP cells. Subsequent prediction using bioinformatics analysis revealed that the altered miRNAs were functionally involved in several cell signaling pathways, including the mitogen-activated protein kinase and WNT pathways. Overall, these results indicated that ROS-mediated cellular damage was inhibited by troxerutin and suggested that the use of troxerutin may be an effective approach in the treatment of alopecia.
authors.affiliation: Molecular‑Targeted Drug Research Center and Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 143‑701, Republic of Korea.
authors.forename: Kyung Mi
authors.initials: KM
authors.lastname: Lim
authors.affiliation: Molecular‑Targeted Drug Research Center and Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 143‑701, Republic of Korea.
authors.forename: Sungkwan
authors.initials: S
authors.lastname: An
authors.affiliation: Molecular‑Targeted Drug Research Center and Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 143‑701, Republic of Korea.
authors.forename: Ok-Kyu
authors.initials: OK
authors.lastname: Lee
authors.affiliation: Molecular‑Targeted Drug Research Center and Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 143‑701, Republic of Korea.
authors.forename: Myung Joo
authors.initials: MJ
authors.lastname: Lee
authors.affiliation: Coreana Cosmetics Co., Ltd., Cheonan, Chungcheong 330‑882, Republic of Korea.
authors.forename: Jeong Pyo
authors.initials: JP
authors.lastname: Lee
authors.affiliation: Coreana Cosmetics Co., Ltd., Cheonan, Chungcheong 330‑882, Republic of Korea.
authors.forename: Kwang Sik
authors.initials: KS
authors.lastname: Lee
authors.affiliation: Coreana Cosmetics Co., Ltd., Cheonan, Chungcheong 330‑882, Republic of Korea.
authors.forename: Ghang Tai
authors.initials: GT
authors.lastname: Lee
authors.affiliation: Coreana Cosmetics Co., Ltd., Cheonan, Chungcheong 330‑882, Republic of Korea.
authors.forename: Kun Kook
authors.initials: KK
authors.lastname: Lee
authors.affiliation: Molecular‑Targeted Drug Research Center and Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 143‑701, Republic of Korea.
authors.forename: Seunghee
authors.initials: S
authors.lastname: Bae
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D020405:Dermis / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D018859:Hair Follicle / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D006896:Hydroxyethylrutoside / Q000031:analogs & derivatives* / Q000494:pharmacology; D035683:MicroRNAs / Q000235:genetics*; D017382:Reactive Oxygen Species / Q000378:metabolism; D059467:Transcriptome / Q000187:drug effects*
chemical_list: D000975:Antioxidants; D006896:Hydroxyethylrutoside; D035683:MicroRNAs; D017382:Reactive Oxygen Species; C005865:troxerutin; D006861:Hydrogen Peroxide