title: Sensitive detection for coralyne and mercury ions based on homo-A/T DNA by exonuclease signal amplification.
abstract: Based on specific homo-A/T DNA binding properties, a strategy for coralyne and mercury ions detection was realised by exonuclease-aided signal amplification. Coralyne could specifically bind homo-A DNA and protect it from the hydrolysis of exonuclease I. The coralyne-protected DNA was subsequently used as a trigger strand to hydrolyze DNA2 in exonuclease-aided signal amplification process. Thiazole orange was used to quantify the remainder DNA2. Under the optimal condition, the fluorescence intensity was linearly proportional to the concentration of coralyne in the range of 0.2-100 nM with a limit of detection (LOD) of 0.31 nM, which presented the lowest LOD for coralyne among all reported. With homo-T and Hg(2+) taking the place of homo-A DNA and coralyne, respectively, the system could also be used for Hg(2+) detection. The experiments in real samples also showed good results. This method was label-free, low-cost, easy-operating and highly repeatable for the detection of coralyne and mercury ions. It could also be extended to detect various analytes, such as other metal ions, proteins and small molecules by using appropriate aptamers.
authors.affiliation: Department of Chemistry, and Shanghai Key Lab of Chemical Assessment and Sustainability, Tongji University, Shanghai 200092, China.
authors.forename: Hailiang
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Chemistry, and Shanghai Key Lab of Chemical Assessment and Sustainability, Tongji University, Shanghai 200092, China. Electronic address: shishuo@tongji.edu.cn.
authors.forename: Shuo
authors.initials: S
authors.lastname: Shi
authors.affiliation: Department of Chemistry, and Shanghai Key Lab of Chemical Assessment and Sustainability, Tongji University, Shanghai 200092, China.
authors.forename: Xuyue
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Department of Chemistry, and Shanghai Key Lab of Chemical Assessment and Sustainability, Tongji University, Shanghai 200092, China. Electronic address: tmyao@tongji.edu.cn.
authors.forename: Tianming
authors.initials: T
authors.lastname: Yao
mesh_terms: D001600:Berberine Alkaloids / Q000032:analysis* / Q000737:chemistry; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D015342:DNA Probes / Q000737:chemistry / Q000235:genetics; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005090:Exodeoxyribonucleases / Q000737:chemistry* / Q000235:genetics; D007477:Ions / Q000032:analysis / Q000737:chemistry; D008628:Mercury / Q000032:analysis* / Q000737:chemistry; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*
chemical_list: D001600:Berberine Alkaloids; D015342:DNA Probes; D007477:Ions; C000666:coralyne; D004247:DNA; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III; D008628:Mercury
keywords: Amplification; Coralyne; Exonuclease; Fluorescence; Mercury ions