title: Dispersibility-Dependent Biodegradation of Graphene Oxide by Myeloperoxidase.
abstract: Understanding human health risk associated with the rapidly emerging graphene-based nanomaterials represents a great challenge because of the diversity of applications and the wide range of possible ways of exposure to this type of materials. Herein, the biodegradation of graphene oxide (GO) sheets is reported by using myeloperoxidase (hMPO) derived from human neutrophils in the presence of a low concentration of hydrogen peroxide. The degradation capability of the enzyme on three different GO samples containing different degree of oxidation on their graphenic lattice, leading to a variable dispersibility in aqueous media is compared. hMPO fails in degrading the most aggregated GO, but succeeds to completely metabolize highly dispersed GO samples. The spectroscopy and microscopy analyses provide unambiguous evidence for the key roles played by hydrophilicity, negative surface charge, and colloidal stability of the aqueous GO in their biodegradation by hMPO catalysis.
authors.affiliation: CNRS, Institut de Biologie Moléculaire et Cellulaire, Laboratoire d'Immunopathologie et Chimie Thérapeutique, 67000, Strasbourg, France.
authors.forename: Rajendra
authors.initials: R
authors.lastname: Kurapati
authors.affiliation: CNRS, Institut de Biologie Moléculaire et Cellulaire, Laboratoire d'Immunopathologie et Chimie Thérapeutique, 67000, Strasbourg, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Russier
authors.affiliation: ISIS & icFRC, Université de Strasbourg & CNRS, 8 allée Gaspard Monge, 67000, Strasbourg, France.
authors.forename: Marco A
authors.initials: MA
authors.lastname: Squillaci
authors.affiliation: ISOF-CNR, via Gobetti 101, 40129, Bologna, Italy.
authors.forename: Emanuele
authors.initials: E
authors.lastname: Treossi
authors.affiliation: CNRS, Institut de Biologie Moléculaire et Cellulaire, Laboratoire d'Immunopathologie et Chimie Thérapeutique, 67000, Strasbourg, France.
authors.forename: Cécilia
authors.initials: C
authors.lastname: Ménard-Moyon
authors.affiliation: Departamento de Química Orgánica, Facultad de Ciencias y Tecnologías Químicas-IRICA, Universidad de Castilla-La Mancha, 13071, Ciudad Real, Spain.
authors.forename: Antonio Esaú
authors.initials: AE
authors.lastname: Del Rio-Castillo
authors.affiliation: Departamento de Química Orgánica, Facultad de Ciencias y Tecnologías Químicas-IRICA, Universidad de Castilla-La Mancha, 13071, Ciudad Real, Spain.
authors.forename: Ester
authors.initials: E
authors.lastname: Vazquez
authors.affiliation: ISIS & icFRC, Université de Strasbourg & CNRS, 8 allée Gaspard Monge, 67000, Strasbourg, France.
authors.forename: Paolo
authors.initials: P
authors.lastname: Samorì
authors.affiliation: ISOF-CNR, via Gobetti 101, 40129, Bologna, Italy.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Palermo
authors.affiliation: CNRS, Institut de Biologie Moléculaire et Cellulaire, Laboratoire d'Immunopathologie et Chimie Thérapeutique, 67000, Strasbourg, France.
authors.forename: Alberto
authors.initials: A
authors.lastname: Bianco
mesh_terms: D001673:Biodegradation, Environmental; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D010087:Oxides / Q000737:chemistry*; D010316:Particle Size; D009195:Peroxidase / Q000378:metabolism*; D013059:Spectrum Analysis, Raman
chemical_list: D010087:Oxides; D006108:Graphite; D009195:Peroxidase
keywords: biodegradation; biopersistence; graphene oxide; myeloperoxidase; safety