title: Influence of edaphic, climatic, and agronomic factors on the composition and abundance of nitrifying microorganisms in the rhizosphere of commercial olive crops.
abstract: The microbial ecology of the nitrogen cycle in agricultural soils is an issue of major interest. We hypothesized a major effect by farm management systems (mineral versus organic fertilizers) and a minor influence of soil texture and plant variety on the composition and abundance of microbial nitrifiers. We explored changes in composition (16S rRNA gene) of ammonia-oxidizing archaea (AOA), bacteria (AOB), and nitrite-oxidizing bacteria (NOB), and in abundance of AOA and AOB (qPCR of amoA genes) in the rhizosphere of 96 olive orchards differing in climatic conditions, agricultural practices, soil properties, and olive variety. Majority of archaea were 1.1b thaumarchaeota (soil crenarchaeotic group, SCG) closely related to the AOA genus Nitrososphaera. Most AOB (97%) were identical to Nitrosospira tenuis and most NOB (76%) were closely related to Nitrospira sp. Common factors shaping nitrifiers assemblage composition were pH, soil texture, and olive variety. AOB abundance was positively correlated with altitude, pH, and clay content, whereas AOA abundances showed significant relationships with organic nitrogen content and exchangeable K. The abundances of AOA differed significantly among soil textures and olive varieties, and those of AOB among soil management systems and olive varieties. Overall, we observed minor effects by orchard management system, soil cover crop practices, plantation age, or soil organic matter content, and major influence of soil texture, pH, and olive tree variety.
authors.affiliation: Biogeodynamics & Biodiversity Group, Centro de Estudios Avanzados de Blanes, CEAB-CSIC, Blanes, Girona.
authors.forename: Joan
authors.initials: J
authors.lastname: Caliz
authors.affiliation: Institute for Sustainable Agriculture, Spanish National Research Council (CSIC), Campus de Excelencia Internacional Agroalimentario (ceiA3), Córdoba, Spain.
authors.forename: Miguel
authors.initials: M
authors.lastname: Montes-Borrego
authors.affiliation: Biogeodynamics & Biodiversity Group, Centro de Estudios Avanzados de Blanes, CEAB-CSIC, Blanes, Girona.
authors.forename: Xavier
authors.initials: X
authors.lastname: Triadó-Margarit
authors.affiliation: Tallinn University, Institute of Mathematics and Natural Sciences, Narva mnt 25, Tallinn 10120, Estonia.
authors.forename: Madis
authors.initials: M
authors.lastname: Metsis
authors.affiliation: Institute for Sustainable Agriculture, Spanish National Research Council (CSIC), Campus de Excelencia Internacional Agroalimentario (ceiA3), Córdoba, Spain.
authors.forename: Blanca B
authors.initials: BB
authors.lastname: Landa
authors.affiliation: Biogeodynamics & Biodiversity Group, Centro de Estudios Avanzados de Blanes, CEAB-CSIC, Blanes, Girona.
authors.forename: Emilio O
authors.initials: EO
authors.lastname: Casamayor
mesh_terms: D001105:Archaea / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D002980:Climate; D018556:Crops, Agricultural; D006863:Hydrogen-Ion Concentration; D058465:Nitrification; D031658:Olea; D012988:Soil Microbiology