title: Host interactions of Chandipura virus matrix protein.
abstract: The rhabdovirus matrix (M) protein is a multifunctional virion protein that plays major role in virus assembly and budding, virus-induced inhibition of host gene expression and cytopathic effects observed in infected cells. The myriad roles played by this protein in the virus biology make it a critical player in viral pathogenesis. Therefore, discerning the interactions of this protein with host can greatly facilitate our understanding of virus infections, ultimately leading to both improved therapeutics and insight into cellular processes. Chandipura virus (CHPV; Family Rhabdoviridae, Genus Vesiculovirus) is an emerging rhabdovirus responsible for several outbreaks of fatal encephalitis among children in India. The present study aims to screen the human fetal brain cDNA library for interactors of CHPV M protein using yeast two-hybrid system. Ten host protein interactors were identified, three of which were further validated by affinity pull down and protein interaction ELISA. The study identified novel human host interactors for CHPV which concurred with previously described associations in other human viruses.
authors.affiliation: Center for Emerging Diseases, Department of Biotechnology, Jaypee Institute of Information Technology, Noida, UP 201307, India.
authors.forename: Sreejith
authors.initials: S
authors.lastname: Rajasekharan
authors.affiliation: Center for Emerging Diseases, Department of Biotechnology, Jaypee Institute of Information Technology, Noida, UP 201307, India.
authors.forename: Kapila
authors.initials: K
authors.lastname: Kumar
authors.affiliation: Center for Emerging Diseases, Department of Biotechnology, Jaypee Institute of Information Technology, Noida, UP 201307, India.
authors.forename: Jyoti
authors.initials: J
authors.lastname: Rana
authors.affiliation: Department of Biochemistry, University of Delhi South Campus, Benito Juarez Marg, New Delhi 110021, India.
authors.forename: Amita
authors.initials: A
authors.lastname: Gupta
authors.affiliation: Department of Biochemistry, University of Delhi South Campus, Benito Juarez Marg, New Delhi 110021, India.
authors.forename: Vijay K
authors.initials: VK
authors.lastname: Chaudhary
authors.affiliation: Center for Emerging Diseases, Department of Biotechnology, Jaypee Institute of Information Technology, Noida, UP 201307, India. Electronic address: sjay1908@gmail.com.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Gupta
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D019204:GTP-Binding Proteins / Q000378:metabolism; D015723:Gene Library; D054884:Host-Pathogen Interactions; D006801:Humans; D007194:India / Q000453:epidemiology; D016547:Kinesins / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D010749:Phosphoprotein Phosphatases / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae; D050052:TRPC Cation Channels / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism; D020798:Two-Hybrid System Techniques; D018116:Vesiculovirus / Q000235:genetics / Q000378:metabolism*; D014763:Viral Matrix Proteins / Q000378:metabolism*
chemical_list: C501917:ABCE1 protein, human; D018528:ATP-Binding Cassette Transporters; D004268:DNA-Binding Proteins; C500506:KIF5A protein, human; C433768:M protein, chandipura virus; D008565:Membrane Proteins; D016601:RNA-Binding Proteins; C477058:SSBP2 protein, human; C581204:TMEM45A protein, human; D050052:TRPC Cation Channels; C550321:TRPC4AP protein, human; C579432:Thoc2 protein, human; D014404:Tubulin; D014763:Viral Matrix Proteins; C078418:developmentally regulated GTP-binding protein; C499225:CTDNEP1 protein, human; D010749:Phosphoprotein Phosphatases; D019204:GTP-Binding Proteins; D016547:Kinesins
keywords: Chandipura virus; Matrix protein; Protein interactions; Rhabdovirus; Virus–host interactions