title: Nitric oxide ameliorates zinc oxide nanoparticles-induced phytotoxicity in rice seedlings.
abstract: Nitric oxide (NO) has been found to function in enhancing plant tolerance to various environmental stresses. However, role of NO in relieving zinc oxide nanoparticles (ZnO NPs)-induced phytotoxicity remains unknown. Here, sodium nitroprusside (SNP, a NO donor) was used to investigate the possible roles and the regulatory mechanisms of NO in counteracting ZnO NPs toxicity in rice seedlings. Our results showed that 10 μM SNP significantly inhibited the appearance of ZnO NP toxicity symptoms. SNP addition significantly reduced Zn accumulation, reactive oxygen species production and lipid peroxidation caused by ZnO NPs. The protective role of SNP in reducing ZnO NPs-induced oxidative damage is closely related to NO-mediated antioxidant system. A decrease in superoxide dismutase activity, as well as an increase in reduced glutathione content and peroxidase, catalase and ascorbate peroxidase activity was observed under SNP and ZnO NPs combined treatments, compared to ZnO NPs treatment alone. The relative transcript abundance of corresponding antioxidant genes exhibited a similar change. The role of NO in enhancing ZnO NPs tolerance was further confirmed by genetic analysis using a NO excess mutant (noe1) and an OsNOA1-silenced plant (noa1) of rice. Together, this study provides the first evidence indicating that NO functions in ameliorating ZnO NPs-induced phytotoxicity.
authors.affiliation: Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian 361005, China; State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
authors.affiliation: Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian 361005, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian 361005, China.
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Yin
authors.affiliation: Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian 361005, China.
authors.forename: Xiu-Ling
authors.initials: XL
authors.lastname: Li
authors.affiliation: Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian 361005, China; Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang Province 310021, China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Hu
authors.affiliation: Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian 361005, China.
authors.forename: Martin
authors.initials: M
authors.lastname: Simon
authors.affiliation: Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian 361005, China.
authors.forename: Zhi-Jun
authors.initials: ZJ
authors.lastname: Shen
authors.affiliation: Laboratory of Biological Resources Protection and Utilization of Hubei Province, Hubei Institutes for Nationalities, Enshi, Hubei 445000, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xiao
authors.affiliation: State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Cheng-Cai
authors.initials: CC
authors.lastname: Chu
authors.affiliation: College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Xin-Xiang
authors.initials: XX
authors.lastname: Peng
authors.affiliation: Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian 361005, China. Electronic address: zhenghl@xmu.edu.cn.
authors.forename: Hai-Lei
authors.initials: HL
authors.lastname: Zheng
mesh_terms: D000975:Antioxidants / Q000737:chemistry; D060387:Ascorbate Peroxidases / Q000737:chemistry; D018533:Biomass; D002374:Catalase / Q000737:chemistry; D002734:Chlorophyll / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D018506:Gene Expression Regulation, Plant; D005978:Glutathione / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D015227:Lipid Peroxidation; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D009154:Mutation; D009569:Nitric Oxide / Q000737:chemistry*; D012275:Oryza / Q000187:drug effects* / Q000235:genetics; D018384:Oxidative Stress; D009195:Peroxidase / Q000737:chemistry; D018517:Plant Roots / Q000187:drug effects; D018520:Plant Shoots / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000737:chemistry; D036226:Seedlings / Q000187:drug effects; D015032:Zinc / Q000737:chemistry; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D002734:Chlorophyll; D009569:Nitric Oxide; D006861:Hydrogen Peroxide; D060387:Ascorbate Peroxidases; D002374:Catalase; D009195:Peroxidase; D005978:Glutathione; D015032:Zinc; D015034:Zinc Oxide
keywords: Antioxidant; Nitric oxide; Phytotoxicity; Rice; Zinc oxide nanoparticles