title: Effect of Baliospermum montanum nanomedicine apoptosis induction and anti-migration of prostate cancer cells.
abstract: Prostate cancer has been diagnosed as the second most frequent and the sixth among the cancer causing deaths among men worldwide. There is a limited scope for the prevalent therapies as prostate cancer advances and they present adverse aftermaths that have put way for us to delve into naturally available anticancer agents. The main objective of the present work is to compile the advantages of ayurvedic herbal formulations with modern technology. Baliospermum montanum is a plant that is used in ayurveda for the treatment of cancer and the plant is studied to possess various constituents in it that are responsible for its anticancer activity. Stable nanoparticles of B. montanum were prepared from both the aqueous and ethanolic extracts of the plant and its cytotoxic effects were studied on prostate cancer and normal cell lines. Size analysis by DLS and SEM revealed the average size of nanoparticles prepared was 100±50 nm and 150±50 nm for the nanoparticles prepared from aqueous and ethanolic extract respectively. In vitro cytotoxicity showed a concentration and time dependent toxicity on prostate cancer cells with cell viability of 22% and 6% with maximum concentration of aqueous and ethanolic nanoparticles respectively, in 48 h. In vitro hemolysis assay confirmed that the prepared nanoparticles were compatible with blood with no occurrence of hemolysis. The nanoparticles showed a significant reduction in the colony forming ability and wound healing capacity of the prostate cancer cells. These studies hold the anti cancer potential of the B. montanum nanoparticles making it an important candidate for prostate cancer therapy.
authors.affiliation: Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham, Kochi Campus, Kerala 682041, India.
authors.forename: Aleena Mary
authors.initials: AM
authors.lastname: Cherian
authors.affiliation: Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham, Kochi Campus, Kerala 682041, India.
authors.forename: K S
authors.initials: KS
authors.lastname: Snima
authors.affiliation: Holistic Medicine Department, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham, Kochi Campus, Kerala 682041, India.
authors.forename: C Ravindranath
authors.initials: CR
authors.lastname: Kamath
authors.affiliation: Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham, Kochi Campus, Kerala 682041, India.
authors.forename: Shantikumar V
authors.initials: SV
authors.lastname: Nair
authors.affiliation: Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham, Kochi Campus, Kerala 682041, India. Electronic address: vinoth.lakshmanan@gmail.com.
authors.forename: Vinoth-Kumar
authors.initials: VK
authors.lastname: Lakshmanan
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002999:Clone Cells; D019665:Euphorbiaceae / Q000737:chemistry*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D008297:Male; D051379:Mice; D041681:NIH 3T3 Cells; D050997:Nanomedicine; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D010316:Particle Size; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D011471:Prostatic Neoplasms / Q000473:pathology*; D017550:Spectroscopy, Fourier Transform Infrared; D014410:Tumor Stem Cell Assay
chemical_list: D010936:Plant Extracts
keywords: Alternative medicine; Baliospermum montanum; Cancer therapy; Nanoparticles; Prostate cancer