title: Effect of lithium chloride on endoplasmic reticulum stress-related PERK/ROCK signaling in a rat model of glaucoma.
abstract: Elevated intraocular pressure (IOP) is considered as the major risk factor for the loss of retinal ganglion cells (RGCs) and their axons in glaucoma. Lithium chloride (LiCl) inhibits glycogen synthase kinase-3 beta (GSK-3β) and attends PERK-induced endoplasmic reticulum stress (ERs) transition. PERK is a type I transmembrane protein located in the endoplasmic reticulum. PERK pathway activation takes place in ERs early inhibiting protein synthnesis to protect cell and promote cell survival. Here, we firstly evaluate that LiCl reduced IOP when administered intraperitoneally. After 6 weeks, IOP dropped by around 21.9% in LiCl treated rats. Then we investigated the effects of LiCI on PERK-mediated signaling pathways. LiCl treatment activated PERK and inhibited the expression of ROCK-1 and ROCK-2 in a rat model of glaucoma. Collectively, these results suggest that LiCl reduced the IOP through the phosphorylation of PERK by the regulation of PERK/ROCK signaling in glaucoma rat model.
authors.forename: Xiao-Bo
authors.initials: XB
authors.lastname: Sun
authors.forename: Hong-E
authors.initials: HE
authors.lastname: Lu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Chen
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Fan
authors.forename: Bin
authors.initials: B
authors.lastname: Tong
mesh_terms: D051702:Activating Transcription Factor 6 / Q000096:biosynthesis / Q000378:metabolism; D000818:Animals; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects*; D005901:Glaucoma / Q000188:drug therapy / Q000503:physiopathology*; D007429:Intraocular Pressure / Q000187:drug effects; D018021:Lithium Chloride / Q000494:pharmacology*; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects*; D051742:Transcription Factor CHOP / Q000096:biosynthesis / Q000378:metabolism; D019892:eIF-2 Kinase / Q000096:biosynthesis / Q000187:drug effects*; D054460:rho-Associated Kinases / Q000096:biosynthesis / Q000187:drug effects*
chemical_list: D051702:Activating Transcription Factor 6; D018021:Lithium Chloride; D012333:RNA, Messenger; D051742:Transcription Factor CHOP; D019892:eIF-2 Kinase; D054460:rho-Associated Kinases; C497113:Atf6 protein, rat; C497251:Ddit3 protein, rat