title: Activity of Krebs cycle enzymes in mdx mice.
abstract: BACKGROUND
  Duchenne muscular dystrophy (DMD) is a degenerative disease of skeletal, respiratory, and cardiac muscles caused by defects in the dystrophin gene. More recently, brain involvement has been verified. Mitochondrial dysfunction and oxidative stress may underlie the pathophysiology of DMD. In this study we evaluate Krebs cycle enzymes activity in the cerebral cortex, diaphragm, and quadriceps muscles of mdx mice.
  
  
  METHODS
  Cortex, diaphragm, and quadriceps tissues from male dystrophic mdx and control mice were used.
  
  
  RESULTS
  We observed increased malate dehydrogenase activity in the cortex; increased malate dehydrogenase and succinate dehydrogenase activities in the diaphragm; and increased citrate synthase, isocitrate dehydrogenase, and malate dehydrogenase activities in the quadriceps of mdx mice.
  
  
  CONCLUSIONS
  This study showed increased activity of Krebs cycle enzymes in cortex, quadriceps, and diaphragm in mdx mice.
authors.affiliation: Laboratory of Experimental Neuroscience, Postgraduate Program in Health Sciences, University of Southern Santa Catarina, Palhoça, Santa Catarina, Brazil.
authors.forename: Clarissa M
authors.initials: CM
authors.lastname: Comim
authors.affiliation: Laboratory of Experimental Neuroscience, Postgraduate Program in Health Sciences, University of Southern Santa Catarina, Palhoça, Santa Catarina, Brazil.
authors.forename: Andreza
authors.initials: A
authors.lastname: Hoepers
authors.affiliation: Laboratory of Experimental Neuroscience, Postgraduate Program in Health Sciences, University of Southern Santa Catarina, Palhoça, Santa Catarina, Brazil.
authors.forename: Letícia
authors.initials: L
authors.lastname: Ventura
authors.affiliation: Laboratory of Experimental Neuroscience, Postgraduate Program in Health Sciences, University of Southern Santa Catarina, Palhoça, Santa Catarina, Brazil.
authors.forename: Viviane
authors.initials: V
authors.lastname: Freiberger
authors.affiliation: Laboratory of Neurosciences, Graduate Program in Health Sciences, Health Sciences Unit, University of Southern Santa Catarina, Criciúma, Santa Catarina, Brazil.
authors.forename: Diogo
authors.initials: D
authors.lastname: Dominguini
authors.affiliation: Laboratory of Neurosciences, Graduate Program in Health Sciences, Health Sciences Unit, University of Southern Santa Catarina, Criciúma, Santa Catarina, Brazil.
authors.forename: Francielle
authors.initials: F
authors.lastname: Mina
authors.affiliation: Laboratory of Neurosciences, Graduate Program in Health Sciences, Health Sciences Unit, University of Southern Santa Catarina, Criciúma, Santa Catarina, Brazil.
authors.forename: Bruna P
authors.initials: BP
authors.lastname: Mendonça
authors.affiliation: Laboratory of Bioenergetics, Postgraduate Program in Health Sciences, Health Sciences Unit, University of Southern Santa Catarina, 88806-000, Criciúma, Santa Catarina, Brazil.
authors.forename: Giselli
authors.initials: G
authors.lastname: Scaini
authors.affiliation: Human Genome Research Center, Biosciences Institute, University of São Paulo, São Paulo, São Paulo, Brazil.
authors.forename: Mariz
authors.initials: M
authors.lastname: Vainzof
authors.affiliation: Laboratory of Bioenergetics, Postgraduate Program in Health Sciences, Health Sciences Unit, University of Southern Santa Catarina, 88806-000, Criciúma, Santa Catarina, Brazil.
authors.forename: Emílio L
authors.initials: EL
authors.lastname: Streck
authors.affiliation: Laboratory of Neurosciences, Graduate Program in Health Sciences, Health Sciences Unit, University of Southern Santa Catarina, Criciúma, Santa Catarina, Brazil.|Center for Experimental Models in Psychiatry, Department of Psychiatry and Behavioral Sciences, University of Texas Medical School at Houston, Houston, Texas, USA.
authors.forename: João
authors.initials: J
authors.lastname: Quevedo
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000201:enzymology; D002950:Citrate (si)-Synthase / Q000378:metabolism*; D002952:Citric Acid Cycle / Q000502:physiology*; D003964:Diaphragm / Q000201:enzymology; D004195:Disease Models, Animal; D007521:Isocitrate Dehydrogenase / Q000378:metabolism*; D008291:Malate Dehydrogenase / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D018482:Muscle, Skeletal / Q000201:enzymology; D020388:Muscular Dystrophy, Duchenne / Q000201:enzymology* / Q000235:genetics
chemical_list: D008291:Malate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D002950:Citrate (si)-Synthase
keywords: Duchenne muscular dystrophy; Krebs cycle enzymes; cortex; diaphragm; mdx mice; quadriceps