title: Solution NMR Structure Determination of Polytopic α-Helical Membrane Proteins: A Guide to Spin Label Paramagnetic Relaxation Enhancement Restraints.
abstract: Solution nuclear magnetic resonance structures of polytopic α-helical membrane proteins require additional restraints beyond the traditional Nuclear Overhauser Effect (NOE) restraints. Several methods have been developed and this review focuses on paramagnetic relaxation enhancement (PRE). Important aspects of spin labeling, PRE measurements, structure calculations, and structural quality are discussed.
authors.affiliation: Department of Chemistry, University of Virginia, Charlottesville, Virginia, USA. Electronic address: lcolumbus@gmail.com.
authors.forename: Linda
authors.initials: L
authors.lastname: Columbus
authors.affiliation: Department of Biochemistry and Center for Structural Biology, Vanderbilt University School of Medicine, Nashville, Tennessee, USA.
authors.forename: Brett
authors.initials: B
authors.lastname: Kroncke
mesh_terms: D000818:Animals; D006801:Humans; D008958:Models, Molecular; D009589:Nitrogen Oxides / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry*; D013113:Spin Labels / Q000138:chemical synthesis
chemical_list: D009589:Nitrogen Oxides; D011506:Proteins; D013113:Spin Labels
keywords: Membrane proteins; Nitroxide spin label; Nuclear magnetic resonance; Paramagnetic relaxation enhancement; Polytopic; Site-directed spin labeling; α-Helical