title: Ostericum koreanum Reduces LPS-Induced Bone Loss Through Inhibition of Osteoclastogenesis.
abstract: The roots of Ostericum koreanum (OK) Maximowicz have traditionally been used to produce an herbal medicine reported to possess anti-inflammatory, anti-oxidant, antimicrobial, and antitumor activities; however, its effect on bone metabolism has not yet been reported. The present study examined the effects of OK extract on lipopolysaccharide (LPS)-induced bone loss in mice by investigating bone structure and the levels of the receptor activator of nuclear factor kappa-B ligand (RANKL) and osteoprotegerin (OPG) in serum and bone marrow fluid (BMF). The effects of OK extract on osteoclastogenesis were also investigated in mouse bone marrow macrophages by examining the formation of tartrate-resistant acid phosphatase (TRAP)-positive cells, the actin ring, and bone resorption activity. OK reduced LPS-induced bone destruction in vivo via a decrease in the RANKL/OPG ratio. Furthermore, it suppressed the formation of TRAP-positive cells and the actin ring, and reduced the bone-resorbing activity of mature osteoclasts. OK also significantly down-regulated the expression of various osteoclast-specific genes. However, it did not affect osteoblast differentiation, or the expression of genes involved in this process. These results demonstrated that OK prevented LPS-induced bone loss by decreasing the RANKL/OPG ratio in serum and BMF, and inhibited osteoclast differentiation and function, suggesting that OK represents a potential therapeutic drug for the treatment of osteoclast-associated bone diseases.
authors.affiliation: Imaging Science-Based Lung and Bone Diseases Research Center, Iksan, Republic of Korea.
authors.forename: Ju-Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Sung-Jun
authors.initials: SJ
authors.lastname: Ahn
authors.forename: Jong Min
authors.initials: JM
authors.lastname: Baek
authors.forename: Kwon-Ha
authors.initials: KH
authors.lastname: Yoon
authors.forename: Myeung Su
authors.initials: MS
authors.lastname: Lee
authors.forename: Jaemin
authors.initials: J
authors.lastname: Oh
mesh_terms: D000818:Animals; D019661:Apiaceae; D001853:Bone Marrow / Q000378:metabolism; D001862:Bone Resorption / Q000139:chemically induced / Q000188:drug therapy* / Q000235:genetics*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000187:drug effects; D008070:Lipopolysaccharides; D008297:Male; D008813:Mice, Inbred ICR; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects; D053244:Osteoprotegerin / Q000097:blood* / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use*; D053245:RANK Ligand / Q000097:blood* / Q000378:metabolism
chemical_list: D008070:Lipopolysaccharides; D053244:Osteoprotegerin; D010936:Plant Extracts; D053245:RANK Ligand
keywords: Actin Ring; Bone Loss; Bone Resorption; Osteoclast Differentiation; Ostericum koreanum