title: Ciliatoxicity in human primary bronchiolar epithelial cells after repeated exposure at the air-liquid interface with native mainstream smoke of K3R4F cigarettes with and without charcoal filter.
abstract: Mucociliary clearance is the primary physical mechanism to protect the human airways against harmful effects of inhaled particles. Environmental factors play a significant role in the impairment of this defense mechanism, whereas cigarette smoke is discussed to be one of the clinically most important causes. Impaired mucociliary clearance in smokers has been connected to changes in ciliated cells such as decreased numbers, altered structure and beat frequency. Clinical studies have shown that cilia length is reduced in healthy smokers and that long-term exposure to cigarette smoke leads to reduced numbers of ciliated cells in mice. We present an in vitro model of primary normal human bronchiolar epithelial (NHBE) cells with in vivo like morphology to study the influence of cigarette mainstream smoke on ciliated cells. We exposed mucociliary differentiated cultures repeatedly to non-toxic concentrations of mainstream cigarette smoke (4 cigarettes, 5 days/week, 8 repetitions in total) at the air-liquid interface. Charcoal filter tipped cigarettes were compared to those being equipped with standard cellulose acetate filters. Histopathological analyses of the exposed cultures showed a reduction of cilia bearing cells, shortening of existing cilia and finally disappearance of all cilia in cigarette smoke exposed cells. In cultures exposed to charcoal filtered cigarette smoke, little changes in cilia length were seen after four exposure repetitions, but those effects were reversed after a two day recovery period. Those differences indicate that volatile organic compounds, being removed by the charcoal filter tip, affect primary bronchiolar epithelial cells concerning their cilia formation and function comparable with the in vivo situation. In conclusion, our in vitro model presents a valuable tool to study air-borne ciliatoxic compounds.
authors.affiliation: Cultex Laboratories GmbH, Feodor-Lynen-Str. 21, 30625 Hannover, Germany. Electronic address: m.aufderheide@cultex-laboratories.com.
authors.forename: Michaela
authors.initials: M
authors.lastname: Aufderheide
authors.affiliation: Cultex Laboratories GmbH, Feodor-Lynen-Str. 21, 30625 Hannover, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Scheffler
authors.affiliation: Japan Tobacco Inc., 6-2, Umegaoka, Aobaku, Yokohama 227-8512, Kanagawa, Japan.
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Ito
authors.affiliation: Japan Tobacco Inc., 6-2, Umegaoka, Aobaku, Yokohama 227-8512, Kanagawa, Japan.
authors.forename: Shinkichi
authors.initials: S
authors.lastname: Ishikawa
authors.affiliation: Cultex Laboratories GmbH, Feodor-Lynen-Str. 21, 30625 Hannover, Germany.
authors.forename: Makito
authors.initials: M
authors.lastname: Emura
mesh_terms: D000368:Aged; D055745:Bronchioles / Q000187:drug effects / Q000473:pathology; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D002923:Cilia / Q000187:drug effects* / Q000473:pathology; D004847:Epithelial Cells / Q000187:drug effects*; D006801:Humans; D008297:Male; D020545:Respiratory Mucosa / Q000187:drug effects* / Q000473:pathology; D012906:Smoke / Q000009:adverse effects*; D062789:Tobacco Products / Q000009:adverse effects; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*
chemical_list: D012906:Smoke; D014028:Tobacco Smoke Pollution
keywords: Air–liquid interface; CULTEX(®) RFS; Cilia; Ciliatoxic effects; Long-term exposure; Normal bronchial epithelial cells