title: Gender-related effects of sex steroids on histamine release and FcεRI expression in rat peritoneal mast cells.
abstract: Mast cells (MCs) are versatile effector and regulatory cells in various physiologic, immunologic, and pathologic processes. In addition to the well-characterized IgE/FcεRI-mediated degranulation, a variety of biological substances can induce MCs activation and release of their granule content. Sex steroids, mainly estradiol and progesterone, have been demonstrated to elicit MCs activation. Most published studies have been conducted on MCs lines or freshly isolated peritoneal and bone marrow-derived MC without addressing gender impact on MC response. Our goal was to investigate if the effect of estradiol, progesterone, testosterone, and dihydrotestosterone (DHT) on MCs may differ depending on whether female or male rats are used as MCs donors. Our results demonstrated that effect of sex steroids on MCs histamine release is dose- and gender-dependent and can be direct, synergistic, or inhibitory depending on whether hormones are used alone or to pretreat MCs followed by substance P-stimulation or upon IgE-mediated stimulation. In contrast, sex steroids did not have effect on the MC expression of the IgE high affinity receptor, FcεRI, no matter female or male rats were used. In conclusion, MCs degranulation is modulated by sex hormones in a gender-selective fashion, with MC from females being more susceptible than MC from males to the effects of sex steroids.
authors.affiliation: Unidad de Investigación Médica en Enfermedades Infecciosas y Parasitarias, Instituto Mexicano del Seguro Social, 06720 México, DF, Mexico.
authors.forename: Samira
authors.initials: S
authors.lastname: Muñoz-Cruz
authors.affiliation: Unidad de Investigación Médica en Enfermedades Infecciosas y Parasitarias, Instituto Mexicano del Seguro Social, 06720 México, DF, Mexico.
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Mendoza-Rodríguez
authors.affiliation: Centro de Investigación Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, 62100 Cuernavaca, MOR, Mexico.
authors.forename: Karen E
authors.initials: KE
authors.lastname: Nava-Castro
authors.affiliation: Unidad de Investigación Médica en Enfermedades Infecciosas y Parasitarias, Instituto Mexicano del Seguro Social, 06720 México, DF, Mexico.
authors.forename: Lilián
authors.initials: L
authors.lastname: Yepez-Mulia
authors.affiliation: Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Apartado Postal 70228, 04510 México, DF, Mexico.
authors.forename: Jorge
authors.initials: J
authors.lastname: Morales-Montor
mesh_terms: D000818:Animals; D015550:Cell Degranulation / Q000276:immunology; D002478:Cells, Cultured; D013196:Dihydrotestosterone / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D012739:Gonadal Steroid Hormones / Q000494:pharmacology*; D006636:Histamine Release / Q000187:drug effects*; D007073:Immunoglobulin E / Q000276:immunology; D008297:Male; D008407:Mast Cells / Q000166:cytology / Q000276:immunology*; D010537:Peritoneum / Q000166:cytology*; D011374:Progesterone / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017455:Receptors, IgE / Q000096:biosynthesis*; D012737:Sex Factors; D013373:Substance P / Q000378:metabolism; D013739:Testosterone / Q000494:pharmacology
chemical_list: C553474:FCER1A protein, rat; D012739:Gonadal Steroid Hormones; D017455:Receptors, IgE; D013196:Dihydrotestosterone; D013373:Substance P; D007073:Immunoglobulin E; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol