title: Ginsenoside Rg1 relieves tert-Butyl hydroperoxide-induced cell impairment in mouse microglial BV2 cells.
abstract: Microglial activation plays an important role in neurodegenerative diseases associated with oxidative stress. tert-Butyl hydroperoxide (t-BHP), an analog of hydroperoxide, mimics the oxidative damage to microglial cells. It has been reported that ginsenoside Rg1 (G-Rg1), an active ingredient of Panax ginseng, has anti-stress and anti-inflammatory properties. The present study aims to investigate the ability of G-Rg1 to decrease the t-BHP-mediated cell damage of BV2 microglial cells. We performed flow cytometry assays to facilitate the detection of reactive oxygen species as well as Western blotting analyses and immunofluorescence assays using specific antibodies, such as antibodies against phospho-mitogen-activated protein kinases (p-MAPKs), phospho-nuclear factor-κB (p-NF-κB), B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X (Bax), Caspase-3, autophagy marker light chain 3 (LC3), and Becline-1. We found that treatment with 50 μM G-Rg1 protected microglial cells against oxidative damage induced by 10 μM t-BHP.
authors.affiliation: a Key Laboratory of State Administration of Traditional Chinese Medicine of China, Department of Pathophysiology , School of Medicine, Institute of Brain Research, Jinan University , Guangzhou 510632 , China.|b Department of Internal Neurology , Guangzhou Overseas Chinese Hospital, The First Affiliated Hospital of Jinan University , Guangzhou 510632 , China.
authors.forename: Dan
authors.initials: D
authors.lastname: Lu
authors.affiliation: a Key Laboratory of State Administration of Traditional Chinese Medicine of China, Department of Pathophysiology , School of Medicine, Institute of Brain Research, Jinan University , Guangzhou 510632 , China.
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Zhu
authors.affiliation: a Key Laboratory of State Administration of Traditional Chinese Medicine of China, Department of Pathophysiology , School of Medicine, Institute of Brain Research, Jinan University , Guangzhou 510632 , China.
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Shu
authors.affiliation: a Key Laboratory of State Administration of Traditional Chinese Medicine of China, Department of Pathophysiology , School of Medicine, Institute of Brain Research, Jinan University , Guangzhou 510632 , China.
authors.forename: Chan-Juan
authors.initials: CJ
authors.lastname: Zhang
authors.affiliation: a Key Laboratory of State Administration of Traditional Chinese Medicine of China, Department of Pathophysiology , School of Medicine, Institute of Brain Research, Jinan University , Guangzhou 510632 , China.
authors.forename: Jia-Yi
authors.initials: JY
authors.lastname: Zhao
authors.affiliation: a Key Laboratory of State Administration of Traditional Chinese Medicine of China, Department of Pathophysiology , School of Medicine, Institute of Brain Research, Jinan University , Guangzhou 510632 , China.
authors.forename: Ren-Bin
authors.initials: RB
authors.lastname: Qi
authors.affiliation: a Key Laboratory of State Administration of Traditional Chinese Medicine of China, Department of Pathophysiology , School of Medicine, Institute of Brain Research, Jinan University , Guangzhou 510632 , China.
authors.forename: Hua-Dong
authors.initials: HD
authors.lastname: Wang
authors.affiliation: a Key Laboratory of State Administration of Traditional Chinese Medicine of China, Department of Pathophysiology , School of Medicine, Institute of Brain Research, Jinan University , Guangzhou 510632 , China.
authors.forename: Da-Xiang
authors.initials: DX
authors.lastname: Lu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology*; D001343:Autophagy / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D036145:Ginsenosides / Q000737:chemistry / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D051379:Mice; D017628:Microglia / Q000166:cytology; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D015394:Molecular Structure; D016328:NF-kappa B / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D005894:Panax / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000378:metabolism; D020122:tert-Butylhydroperoxide / Q000494:pharmacology*
chemical_list: D000893:Anti-Inflammatory Agents; D036145:Ginsenosides; D016328:NF-kappa B; D017382:Reactive Oxygen Species; D020122:tert-Butylhydroperoxide; D006861:Hydrogen Peroxide; D020928:Mitogen-Activated Protein Kinases; D053148:Caspase 3; C035054:ginsenoside Rg1
keywords: BV2 microglial cell; apoptosis; autophagy marker light chain 3; ginsenoside Rg1; reactive oxygen species; tert-Butyl hydroperoxide