title: Trafficking of the signature protein of intra-erythrocytic Plasmodium berghei-induced structures, IBIS1, to P. falciparum Maurer's clefts.
abstract: Remodeling of the host red blood cell by Plasmodium falciparum is well established and crucial for infection and parasite virulence. Host cell modifications are not exclusive to human Plasmodium parasites and also occur in hepatocytes and erythrocytes infected with murine Plasmodium parasites. The recently described intra-erythrocytic P. berghei-induced structures (IBIS) share similarities to P. falciparum Maurer's clefts. It is shown here that a potential candidate IBIS1 homologue in P. falciparum, PfHYP12 (PF3D7_1301400), is partially exported into the erythrocyte cytoplasm. To analyze a potential similarity between IBIS and Maurer's clefts we expressed the signature protein of IBIS in P. falciparum parasites. Visualization of the tagged protein revealed that PbIBIS1 can be exported by P. falciparum and localizes to Maurer's clefts in P. falciparum-infected erythrocytes, which indicates that IBIS and Maurer's clefts may be evolutionarily conserved parasite-induced structures in infected erythrocytes.
authors.affiliation: Max Planck Institute for Infection Biology, Parasitology Unit, 10117 Berlin, Germany.
authors.forename: Wiebke
authors.initials: W
authors.lastname: Petersen
authors.affiliation: Max Planck Institute for Infection Biology, Parasitology Unit, 10117 Berlin, Germany; Institute of Biology, Humboldt University, 10117 Berlin, Germany.
authors.forename: Kai
authors.initials: K
authors.lastname: Matuschewski
authors.affiliation: Max Planck Institute for Infection Biology, Parasitology Unit, 10117 Berlin, Germany. Electronic address: ingmundson@mpiib-berlin.mpg.de.
authors.forename: Alyssa
authors.initials: A
authors.lastname: Ingmundson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004912:Erythrocytes / Q000469:parasitology*; D006790:Host-Parasite Interactions; D006801:Humans; D016778:Malaria, Falciparum / Q000469:parasitology*; D051379:Mice; D008969:Molecular Sequence Data; D010962:Plasmodium berghei / Q000235:genetics*; D010963:Plasmodium falciparum / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D021381:Protein Transport; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment
chemical_list: D015800:Protozoan Proteins
keywords: Exported proteins; Host cell remodeling; Malaria; Maurer's clefts; Plasmodium