title: Antiplatelet and antithrombotic activity of a fibrin(ogen)olytic protease from Bacillus cereus strain FF01.
abstract: Fibrin(ogen)olytic enzymes offer great promise for the treatment of thrombosis associated disorders. The present study describes the characterization of an extracellular fibrin(ogen)olytic serine protease (named Bacethrombase) purified from the Bacillus cereus strain FF01. The molecular mass of the Bacethrombase was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis and matrix assisted laser desorption/ionization-time-of-flight-mass spectroscopy analyses at 39.5 kDa and 38,450.51 Da, respectively. The peptide mass fingerprinting and analyses of the composition of the amino acids revealed the similarity of the Bacethrombase to the bacterial serine proteases. The secondary structure of the Bacethrombase was composed of 14% helix, 6.6% beta-sheet, and 79.4% random coil. Bacethrombase was found to contain 48% sialic acid and it preferentially degraded the Aα-chain of fibrinogen, as well as fibrin. The anticoagulant potency of the Bacethrombase was comparable with that of warfarin and heparin, and was corroborated by its fibrinogenolytic activity rather than the inhibition of thrombin, prothrombin or FXa. Bacethrombase demonstrated antiplatelet activity, and dose-dependently inhibited the ADP-induced platelet aggregation. Bacethrombase (10 mg/kg) did not show toxicity after i.v. administration in Wistar rats; however, it revealed an in vivo anticoagulant effect and significantly inhibited the carrageenan-induced in vivo thrombus formation in rats.
authors.affiliation: Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology, School of Sciences, Tezpur University, Tezpur 784028, Assam, India.
authors.forename: Sourav
authors.initials: S
authors.lastname: Majumdar
authors.affiliation: Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology, School of Sciences, Tezpur University, Tezpur 784028, Assam, India.
authors.forename: Sumita
authors.initials: S
authors.lastname: Dutta
authors.affiliation: Indian Institute of Chemical Biology, 4, Raja S.C. Mullick Road, Kolkata 700032, West Bengal, India.
authors.forename: Tanusree
authors.initials: T
authors.lastname: Das
authors.affiliation: Division of Pharmaceutical Technology, Defense Research Laboratory, Tezpur 784001, Assam, India.
authors.forename: Pronobesh
authors.initials: P
authors.lastname: Chattopadhyay
authors.affiliation: Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology, School of Sciences, Tezpur University, Tezpur 784028, Assam, India. Electronic address: akm@tezu.ernet.in.
authors.forename: Ashis K
authors.initials: AK
authors.lastname: Mukherjee
mesh_terms: D000818:Animals; D000991:Antithrombins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001409:Bacillus cereus / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D015951:Factor Xa / Q000737:chemistry; D005343:Fibrinolytic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006031:Glycosylation; D006801:Humans; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011499:Protein Processing, Post-Translational; D017433:Protein Structure, Secondary; D059748:Proteolysis; D017208:Rats, Wistar; D020539:Sequence Analysis, Protein; D013379:Substrate Specificity; D013917:Thrombin / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000991:Antithrombins; D001426:Bacterial Proteins; D005343:Fibrinolytic Agents; D010975:Platelet Aggregation Inhibitors; D013917:Thrombin; D015951:Factor Xa
keywords: Anticoagulant; Antiplatelet; Thrombolytic agent