title: Immunoregulatory Effects of Paeoniflorin Exerts Anti-asthmatic Effects via Modulation of the Th1/Th2 Equilibrium.
abstract: Paeoniflorin has been demonstrated to exert anti-inflammatory and immunomodulatory effects in the animal study. In this study, we investigated immunoregulatory effects of paeoniflorin on anti-asthmatic effects and underlying mechanisms. Asthma model was established by ovalbumin-induced. A total of 50 mice were randomly assigned to five experimental groups: control, model, dexamethasone (2 mg/kg), and paeoniflorin (10 and 20 mg/kg). Airway resistance (Raw) were measured by the forced oscillation technique; histological studies were evaluated by the hematoxylin and eosin (HE) staining; Th1/Th2 cytokines were evaluated by enzyme-linked immunosorbent assay (ELISA); Th1/Th2 cells were evaluated by flow cytometry (FCM); and GATA3 and T-bet were evaluated by Western blot. Our study demonstrated that, compared with model group, paeoniflorin inhibited ovalbumin (OVA)-induced increases in Raw and eosinophil count; interleukin (IL)-4, IgE levels were recovered in bronchoalveolar lavage fluid compared; increased IFN-γ level in bronchoalveolar lavage fluid; histological studies demonstrated that paeoniflorin substantially inhibited OVA-induced eosinophilia in lung tissue and lung tissue compared with model group. Flow cytometry studies demonstrated that paeoniflorin can regulate Th1/Th2 balance. These findings suggest that paeoniflorin may effectively ameliorate the progression of asthma and could be used as a therapy for patients with allergic asthma.
authors.affiliation: Changchun University of Chinese Medicine, Changhcun, 130117, China.
authors.forename: Tianzhu
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Nanjing University of Chinese Medicine, Nanjing, China.
authors.forename: Zhaocong
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Jilin Agricultural University, Changchun, 130118, China.
authors.forename: Shihai
authors.initials: S
authors.lastname: Yang
authors.affiliation: School of Life Science, Peking University, Beijing, China, 100871.
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.affiliation: Changchun University of Chinese Medicine, Changhcun, 130117, China. wangshuminch@126.com.
authors.forename: Shumin
authors.initials: S
authors.lastname: Wang
mesh_terms: D000403:Airway Resistance / Q000187:drug effects; D000818:Animals; D018927:Anti-Asthmatic Agents / Q000494:pharmacology*; D001249:Asthma / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology / Q000378:metabolism / Q000503:physiopathology; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D003907:Dexamethasone / Q000494:pharmacology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005260:Female; D050990:GATA3 Transcription Factor / Q000378:metabolism; D005960:Glucosides / Q000494:pharmacology*; D007073:Immunoglobulin E / Q000378:metabolism; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D008168:Lung / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000503:physiopathology; D008807:Mice, Inbred BALB C; D039821:Monoterpenes / Q000494:pharmacology*; D010047:Ovalbumin; D008517:Phytotherapy; D010946:Plants, Medicinal; D011657:Pulmonary Eosinophilia / Q000139:chemically induced / Q000276:immunology / Q000517:prevention & control; D020825:T-Box Domain Proteins / Q000378:metabolism; D018417:Th1 Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D058408:Th1-Th2 Balance / Q000187:drug effects*; D018418:Th2 Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D000098815:T-bet Transcription Factor; D006027:Glycosides
chemical_list: D018927:Anti-Asthmatic Agents; D003907:Dexamethasone; D050990:GATA3 Transcription Factor; D005960:Glucosides; D007073:Immunoglobulin E; D007371:Interferon-gamma; D015847:Interleukin-4; D039821:Monoterpenes; D010047:Ovalbumin; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; C494748:Gata3 protein, mouse; C015423:peoniflorin; D006027:Glycosides
keywords: GATA3/T-bet; Th1/Th2; anti-asthmatic; paeoniflorin