title: Effect of toluene concentration and hydrogen peroxide on Pseudomonas plecoglossicida cometabolizing mixture of cis-DCE and TCE in soil slurry.
abstract: An indigenous Pseudomonas sp., isolated from the regional contaminated soil and identified as P. plecoglossicida, was evaluated for its aerobic cometabolic removal of cis-1,2-dichloroethylene (cis-DCE) and trichloroethylene (TCE) using toluene as growth substrate in a laboratory-scale soil slurry. The aerobic simultaneous bioremoval of the cis-DCE/TCE/toluene mixture was studied under different conditions. Results showed that an increase in toluene concentration level from 300 to 900 mg/kg prolonged the lag phase for the bacterial growth, while the bioremoval extent for cis-DCE, TCE, and toluene declined as the initial toluene concentration increased. In addition, the cometabolic bioremoval of cis-DCE and TCE was inhibited by the presence of hydrogen peroxide as the additional oxygen source, while the bioremoval of toluene (900 mg/kg) was enhanced after 9 days of incubation. The subsequent addition of toluene did not improve the cometabolic bioremoval of cis-DCE and TCE. The obtained results would help to enhance the applicability of bioremediation technology to the mixed waste contaminated sites.
authors.affiliation: College of Natural Resources and Environmental Science, South China Agricultural University, Guangzhou, 510642, China.|Department of Civil and Environmental Engineering, Faculty of Science and Technology, University of Macau, Macau SAR, 999078, China.|Key Laboratory of the Ministry of Land and Resources for Construction Land Transformation, South China Agricultural University, Guangzhou, 510642, China.|Guangdong Provincial Key Laboratory of Land Use and Consolidation, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Junhui
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Civil and Environmental Engineering, Faculty of Science and Technology, University of Macau, Macau SAR, 999078, China.
authors.forename: Qihong
authors.initials: Q
authors.lastname: Lu
authors.affiliation: Department of Civil and Environmental Engineering, Faculty of Science and Technology, University of Macau, Macau SAR, 999078, China.
authors.forename: Renata Alves
authors.initials: RA
authors.lastname: de Toledo
authors.affiliation: College of Natural Resources and Environmental Science, South China Agricultural University, Guangzhou, 510642, China. luying@scau.edu.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Civil and Environmental Engineering, Faculty of Science and Technology, University of Macau, Macau SAR, 999078, China. hjshim@umac.mo.
authors.forename: Hojae
authors.initials: H
authors.lastname: Shim
mesh_terms: D001673:Biodegradation, Environmental; D004000:Dichloroethylenes / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000737:chemistry*; D011549:Pseudomonas / Q000737:chemistry / Q000378:metabolism*; D012987:Soil / Q000737:chemistry*; D012989:Soil Pollutants / Q000378:metabolism*; D014050:Toluene / Q000737:chemistry*; D014241:Trichloroethylene / Q000378:metabolism*
chemical_list: D004000:Dichloroethylenes; D012987:Soil; D012989:Soil Pollutants; D014241:Trichloroethylene; D014050:Toluene; D006861:Hydrogen Peroxide; C035384:1,2-dichloroethylene
keywords: Aerobic bioremoval; Cometabolism; Mixture; Soil; TCE; Toluene; cis-DCE