title: Protection Provided by an Encapsulated Live Attenuated ΔabcBA Strain of Brucella ovis against Experimental Challenge in a Murine Model.
abstract: This study aimed to evaluate the Brucella ovis ΔabcBA strain as a vaccine candidate in the murine model. BALB/c mice were subcutaneously or intraperitoneally immunized with a single dose or three doses of the B. ovis ΔabcBA strain and then were challenged with wild-type B. ovis. Single or multiple immunizations provided only mild protection, with significantly smaller numbers of wild-type B. ovis CFU in the livers of immunized mice but not in the spleens. Encapsulation of B. ovis ΔabcBA significantly improved protection against experimental challenges in both BALB/c and C57BL/6 mice. Furthermore, immunization with encapsulated B. ovis ΔabcBA markedly prevented lesions in the spleens and livers of experimentally challenged mice. These results demonstrated that the encapsulated B. ovis ΔabcBA strain confers protection to mice; therefore, this strain has potential as a vaccine candidate for rams.
authors.affiliation: Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Ana Patrícia C
authors.initials: AP
authors.lastname: Silva
authors.affiliation: Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Auricélio A
authors.initials: AA
authors.lastname: Macêdo
authors.affiliation: Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Teane M A
authors.initials: TM
authors.lastname: Silva
authors.affiliation: Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Luana C A
authors.initials: LC
authors.lastname: Ximenes
authors.affiliation: Embrapa Gado de Leite, Juiz de Fora, MG, Brazil CiPharma-Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil.
authors.forename: Humberto M
authors.initials: HM
authors.lastname: Brandão
authors.affiliation: Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Tatiane A
authors.initials: TA
authors.lastname: Paixão
authors.affiliation: Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil rsantos@vet.ufmg.br.
authors.forename: Renato L
authors.initials: RL
authors.lastname: Santos
mesh_terms: D000818:Animals; D058491:Bacterial Load; D002004:Brucella Vaccine / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D042061:Brucella ovis / Q000235:genetics / Q000276:immunology*; D002006:Brucellosis / Q000276:immunology / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control*; D018914:Cells, Immobilized / Q000276:immunology; D004195:Disease Models, Animal; D004337:Drug Carriers / Q000008:administration & dosage; D017353:Gene Deletion; D007274:Injections, Intraperitoneal; D007279:Injections, Subcutaneous; D008099:Liver / Q000382:microbiology / Q000473:pathology; D008297:Male; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000382:microbiology / Q000473:pathology; D014611:Vaccination / Q000379:methods*; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D002004:Brucella Vaccine; D004337:Drug Carriers; D014613:Vaccines, Attenuated