title: The tRNA Splicing Endonuclease Complex Cleaves the Mitochondria-localized CBP1 mRNA.
abstract: The tRNA splicing endonuclease (Sen) complex is located on the mitochondrial outer membrane and splices precursor tRNAs in Saccharomyces cerevisiae. Here, we demonstrate that the Sen complex cleaves the mitochondria-localized mRNA encoding Cbp1 (cytochrome b mRNA processing 1). Endonucleolytic cleavage of this mRNA required two cis-elements: the mitochondrial targeting signal and the stem-loop 652-726-nt region. Mitochondrial localization of the Sen complex was required for cleavage of the CBP1 mRNA, and the Sen complex cleaved this mRNA directly in vitro. We propose that the Sen complex cleaves the CBP1 mRNA, which is co-translationally localized to mitochondria via its mitochondrial targeting signal.
authors.affiliation: From the Graduate School of Pharmaceutical Science, Tohoku University, Aoba-ku, Sendai 980-8578, Japan.
authors.forename: Tatsuhisa
authors.initials: T
authors.lastname: Tsuboi
authors.affiliation: From the Graduate School of Pharmaceutical Science, Tohoku University, Aoba-ku, Sendai 980-8578, Japan.
authors.forename: Reina
authors.initials: R
authors.lastname: Yamazaki
authors.affiliation: From the Graduate School of Pharmaceutical Science, Tohoku University, Aoba-ku, Sendai 980-8578, Japan.
authors.forename: Risa
authors.initials: R
authors.lastname: Nobuta
authors.affiliation: From the Graduate School of Pharmaceutical Science, Tohoku University, Aoba-ku, Sendai 980-8578, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Ikeuchi
authors.affiliation: From the Graduate School of Pharmaceutical Science, Tohoku University, Aoba-ku, Sendai 980-8578, Japan.
authors.forename: Shiho
authors.initials: S
authors.lastname: Makino
authors.affiliation: From the Graduate School of Pharmaceutical Science, Tohoku University, Aoba-ku, Sendai 980-8578, Japan.
authors.forename: Ayumi
authors.initials: A
authors.lastname: Ohtaki
authors.affiliation: the Graduate School of Frontier Sciences, University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Suzuki
authors.affiliation: the Graduate School of Life Science, University of Hyogo, Hyogo 678-1297, Japan, and.
authors.forename: Tohru
authors.initials: T
authors.lastname: Yoshihisa
authors.affiliation: the PTC Therapeutics, South Plainfield, New Jersey 07080.
authors.forename: Christopher
authors.initials: C
authors.lastname: Trotta
authors.affiliation: From the Graduate School of Pharmaceutical Science, Tohoku University, Aoba-ku, Sendai 980-8578, Japan, tinada@m.tohoku.ac.jp.
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Inada
mesh_terms: D001483:Base Sequence; D004722:Endoribonucleases / Q000235:genetics / Q000378:metabolism*; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012326:RNA Splicing; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D024101:Mitochondrial Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; C494475:cbp1 protein, S cerevisiae; D004722:Endoribonucleases; C051012:splicing endonuclease
keywords: endonuclease; mRNA decay; mRNA decay, endonuclease; mRNA localization; mitochondria; quality control; tRNA splicing; transfer RNA (tRNA); translation