title: Resorption of monetite calcium phosphate cement by mouse bone marrow derived osteoclasts.
abstract: Recently the interest for monetite based biomaterials as bone grafts has increased; since in vivo studies have demonstrated that they are degradable, osteoconductive and improve bone healing. So far osteoclastic resorption of monetite has received little attention. The current study focuses on the osteoclastic resorption of monetite cement using primary mouse bone marrow macrophages, which have the potential to differentiate into resorbing osteoclasts when treated with receptor activator NF-κB ligand (RANKL). The osteoclast viability and differentiation were analysed on monetite cement and compared to cortical bovine bone discs. After seven days live/dead stain results showed no significant difference in viability between the two materials. However, the differentiation was significantly higher on the bone discs, as shown by tartrate resistant acid phosphatase (TRAP) activity and Cathepsin K gene expression. Moreover monetite samples with differentiated osteoclasts had a 1.4 fold elevated calcium ion concentration in their culture media compared to monetite samples with undifferentiated cells. This indicates active resorption of monetite in the presence of osteoclasts. In conclusion, this study suggests that osteoclasts have a crucial role in the resorption of monetite based biomaterials. It also provides a useful model for studying in vitro resorption of acidic calcium phosphate cements by primary murine cells.
authors.affiliation: Department of Engineering Sciences, Division of Applied Materials Science, Uppsala University, 751 21 Uppsala, Sweden. Electronic address: maryam.montazerolghaem@angstrom.uu.se.
authors.forename: M
authors.initials: M
authors.lastname: Montazerolghaem
authors.affiliation: Department of Engineering Sciences, Division of Applied Materials Science, Uppsala University, 751 21 Uppsala, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Karlsson Ott
authors.affiliation: Department of Engineering Sciences, Division of Applied Materials Science, Uppsala University, 751 21 Uppsala, Sweden.
authors.forename: H
authors.initials: H
authors.lastname: Engqvist
authors.affiliation: Department of Medical Sciences, Section of Clinical Pharmacology and Osteoporosis, Uppsala University, Uppsala, 751 85 Uppsala, Sweden.
authors.forename: H
authors.initials: H
authors.lastname: Melhus
authors.affiliation: Department of Medical Sciences, Section of Clinical Pharmacology and Osteoporosis, Uppsala University, Uppsala, 751 85 Uppsala, Sweden.
authors.forename: A J
authors.initials: AJ
authors.lastname: Rasmusson
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects*; D001862:Bone Resorption; D002130:Calcium Phosphates / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D008264:Macrophages; D051379:Mice; D008810:Mice, Inbred C57BL; D010010:Osteoclasts / Q000166:cytology* / Q000187:drug effects*; D014961:X-Ray Diffraction
chemical_list: D002130:Calcium Phosphates; C485829:calcium phosphate, dibasic, anhydrous
keywords: Acidic calcium phosphate cement; Bone filler; Bone marrow macrophages; Osteoclastic resorption