title: New compound with DNA Topo I inhibitory activity purified from Penicillium oxalicum HSY05.
abstract: Strain HSY05 was isolated from sea sediment collected from the South China Sea and was later identified as Penicillium oxalicum by 16S rDNA sequence analysis. Various chromatographic processes led to the isolation and purification of two metabolites from the fermentation culture of HSY05, including one new compound, 2,2',4,4'-tetrahyoxy-8'-methyl-6-methoxy-acyl-ethyl-diphenylmethanone (1), and a known compound secalonic acid D (SAD, 2), as characterised by UV, IR, 1D, 2D-NMR and MS data. The inhibitory activities against topoisomerase I of these two compounds were evaluated. The result showed that in addition to the known topo I inhibitor SAD (2), compound 1 also exhibited a moderate inhibitory effect.
authors.affiliation: a School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University , Shenyang 110016 , P.R. China.|b Key Laboratory of Structure-Based Drug Design and Discovery (Shenyang Pharmaceutical University), Ministry of Education , Shenyang 110016 , P.R. China.
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
authors.affiliation: a School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University , Shenyang 110016 , P.R. China.|b Key Laboratory of Structure-Based Drug Design and Discovery (Shenyang Pharmaceutical University), Ministry of Education , Shenyang 110016 , P.R. China.
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Wang
authors.affiliation: a School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University , Shenyang 110016 , P.R. China.|b Key Laboratory of Structure-Based Drug Design and Discovery (Shenyang Pharmaceutical University), Ministry of Education , Shenyang 110016 , P.R. China.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Zhang
authors.affiliation: a School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University , Shenyang 110016 , P.R. China.|b Key Laboratory of Structure-Based Drug Design and Discovery (Shenyang Pharmaceutical University), Ministry of Education , Shenyang 110016 , P.R. China.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.affiliation: a School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University , Shenyang 110016 , P.R. China.|b Key Laboratory of Structure-Based Drug Design and Discovery (Shenyang Pharmaceutical University), Ministry of Education , Shenyang 110016 , P.R. China.
authors.forename: Feng-Jun
authors.initials: FJ
authors.lastname: He
authors.affiliation: a School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University , Shenyang 110016 , P.R. China.|b Key Laboratory of Structure-Based Drug Design and Discovery (Shenyang Pharmaceutical University), Ministry of Education , Shenyang 110016 , P.R. China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Bai
authors.affiliation: a School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University , Shenyang 110016 , P.R. China.|b Key Laboratory of Structure-Based Drug Design and Discovery (Shenyang Pharmaceutical University), Ministry of Education , Shenyang 110016 , P.R. China.
authors.forename: Hui-Ming
authors.initials: HM
authors.lastname: Hua
authors.affiliation: a School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University , Shenyang 110016 , P.R. China.|b Key Laboratory of Structure-Based Drug Design and Discovery (Shenyang Pharmaceutical University), Ministry of Education , Shenyang 110016 , P.R. China.
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.affiliation: a School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University , Shenyang 110016 , P.R. China.|b Key Laboratory of Structure-Based Drug Design and Discovery (Shenyang Pharmaceutical University), Ministry of Education , Shenyang 110016 , P.R. China.
authors.forename: Yue-Hu
authors.initials: YH
authors.lastname: Pei
mesh_terms: D001577:Benzophenones / Q000737:chemistry / Q000302:isolation & purification; D002681:China; D019015:Geologic Sediments / Q000382:microbiology; D015394:Molecular Structure; D009792:Oceans and Seas; D010407:Penicillium / Q000737:chemistry* / Q000302:isolation & purification; D059004:Topoisomerase I Inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D044004:Xanthones / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D001577:Benzophenones; D059004:Topoisomerase I Inhibitors; D044004:Xanthones; C009218:secalonic acid
keywords: DNA topoisomerase I inhibitory activity; Penicillium oxalicum; marine fungal metabolites