title: Effect of ulinastatin on HMGB1 expression in rats with acute lung injury induced by sepsis.
abstract: The aim of this study was to investigate the influence of ulinastatin (UTI) on high mobility group box 1 (HMGB1), tumor necrosis factor (TNF)-α, and interleukin (IL)-6 expression in acute lung injury (ALI) rats with sepsis caused by cecal ligation and puncture (CLP) surgery, as well as to examine the underlying biological mechanism. Thirty rats were randomly and evenly divided into sham (control), CLP, and CLP + UTI groups. Thirty minutes after the surgery, the rats in the CLP + UTI group received UTI via the caudal vein, while normal saline was administered to rats in the other groups. Blood, lung tissues, and bronchoalveolar lavage fluid (BALF) were collected at different time points (6, 12, 24, and 48 h) after surgery for determination of related indicators. Compared with the CLP group, rats in the CLP + UTI group exhibited higher seven day survival rates, less lung injury, and decreased HMGB1 expression in the lung tissue, serum, and BALF. In addition, the levels of TNF-α and IL-6 at 24 h in the CLP + UTI group were markedly lower than those in the CLP group. These results suggest that by deregulation, UTI might decrease the lung injury and increase the survival time of ALI rats by downregulating HMGB1 expression as well as by inhibiting TNF-α and IL-6 levels in serum and BALF.
authors.affiliation: Department of Emergency and Critical Care Medicine, Shanghai Changzheng Hospital, The Second Military Medical University, Shanghai, China.
authors.forename: S Y
authors.initials: SY
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, Shanghai Changzheng Hospital, The Second Military Medical University, Shanghai, China.
authors.forename: Z J
authors.initials: ZJ
authors.lastname: Li
authors.affiliation: Department of Emergency and Critical Care Medicine, Shanghai Changzheng Hospital, The Second Military Medical University, Shanghai, China.
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Emergency and Critical Care Medicine, Shanghai Changzheng Hospital, The Second Military Medical University, Shanghai, China liwenfang201441@126.com.
authors.forename: W F
authors.initials: WF
authors.lastname: Li
authors.affiliation: Department of Emergency and Critical Care Medicine, Shanghai Changzheng Hospital, The Second Military Medical University, Shanghai, China.
authors.forename: Z F
authors.initials: ZF
authors.lastname: Lin
mesh_terms: D055371:Acute Lung Injury / Q000188:drug therapy / Q000209:etiology* / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid; D015870:Gene Expression; D006023:Glycoproteins / Q000494:pharmacology*; D024243:HMGB1 Protein / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D015850:Interleukin-6 / Q000097:blood; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051381:Rats; D018805:Sepsis / Q000150:complications*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000187:drug effects
chemical_list: D006023:Glycoproteins; D024243:HMGB1 Protein; C104200:Hbp1 protein, rat; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; C028665:urinastatin