title: Effects of sulfated and non-sulfated β-glucan extracted from Agaricus brasiliensis in breast adenocarcinoma cells - MCF-7.
abstract: The β-glucans (β-G) are polysaccharides produced by various organisms, and sulfation of β-G renders them more soluble. With the objective to assess the effects of sulfated and non-sulfated β-G extracted from Agaricus brasiliensis in MCF-7 cells, assays were used to evaluate cytotoxicity, genotoxicity, cell proliferation and mRNA expression. The sulfated and non-sulfated β-G showed dose-dependent cytotoxicity at concentrations of 5 and 10 μg/mL, by the MTT assay. However, only cytotoxicity was observed after 24 h by the Red Neutral test for sulfated β-G, with no genotoxicity for either β-G in comet assay. Proliferation was decreased only at 72 h at a concentration of 100 μg/mL of sulfated β-G. Treatment with 5 μg/mL of sulfated β-G for 6 h reduced the expression of pro-apoptotic genes and stress signaling genes, cell cycle arrest, damage and cell migration. The 5 μg/mL of non-sulfated β-G for 6 h reduced the expression of the stress response gene and signaling damage. These results indicate that the cytotoxicity in the MTT is not cell death, and that, in general, sulfated β-G have greater cytotoxicity compared to non-sulfated β-G.
authors.affiliation: a Department of General Biology , State University of Londrina , Londrina , Brazil and.
authors.forename: Adrivanio
authors.initials: A
authors.lastname: Baranoski
authors.affiliation: a Department of General Biology , State University of Londrina , Londrina , Brazil and.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Tempesta Oliveira
authors.affiliation: a Department of General Biology , State University of Londrina , Londrina , Brazil and.
authors.forename: Simone Cristine
authors.initials: SC
authors.lastname: Semprebon
authors.affiliation: a Department of General Biology , State University of Londrina , Londrina , Brazil and.
authors.forename: Andressa Megumi
authors.initials: AM
authors.lastname: Niwa
authors.affiliation: b Department of Pathology, São Paulo State University "Júlio de Mesquita Filho" , São Paulo , Brazil.
authors.forename: Lúcia Regina
authors.initials: LR
authors.lastname: Ribeiro
authors.affiliation: a Department of General Biology , State University of Londrina , Londrina , Brazil and.
authors.forename: Mário Sérgio
authors.initials: MS
authors.lastname: Mantovani
mesh_terms: D000364:Agaricus / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D020552:Comet Assay; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D062610:Fungal Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D061986:MCF-7 Cells; D012333:RNA, Messenger / Q000096:biosynthesis; D047071:beta-Glucans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D062610:Fungal Polysaccharides; D012333:RNA, Messenger; D047071:beta-Glucans
keywords: Cell proliferation; RT-qPCR; cytotoxicity; genotoxicity