title: Pentraxin-3 Silencing Suppresses Gastric Cancer-related Inflammation by Inhibiting Chemotactic Migration of Macrophages.
abstract: BACKGROUND
  Chronic inflammation characterized by the recruitment and activation of macrophages has been implicated in the development of gastric cancer.
  
  
  METHODS
  Expression of the long form of pentraxin-3 (PTX3) in gastric cancer cells was examined by reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay. The migratory capacity of gastric cancer cells and chemotaxis of macrophages by PTX3 were assessed by wound-healing and transwell assays. PTX3 silencing using small interfering RNA (siRNA) was performed to confirm PTX3-mediated effects.
  
  
  RESULTS
  We demonstrated that PTX3 expression was elevated in human advanced gastric cancer tissues with increased infiltration of CD11b+ macrophages. Tumor necrosis factor-alpha increased PTX3 expression via nuclear factor-kappa B activation in human gastric cancer cells. PTX3 promoted the tumor cell migratory potential, the recruitment of macrophages and their subsequent binding to gastric cancer cells. These effects were suppressed by PTX3 knockdown using siRNA.
  
  
  CONCLUSIONS
  Our findings suggest that gastric cancer-derived PTX3 promotes macrophage recruitment, which may contribute to gastric cancer-related inflammation.
authors.affiliation: Department of Biomedical Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea Cell Dysfunction Research Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.
authors.forename: Bongkun
authors.initials: B
authors.lastname: Choi
authors.affiliation: Department of Biomedical Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea Cell Dysfunction Research Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.
authors.forename: Eun-Jin
authors.initials: EJ
authors.lastname: Lee
authors.affiliation: Department of Pathology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.
authors.forename: Young Soo
authors.initials: YS
authors.lastname: Park
authors.affiliation: Department of Biomedical Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea Cell Dysfunction Research Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.
authors.forename: Sang-Min
authors.initials: SM
authors.lastname: Kim
authors.affiliation: Department of Biomedical Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea Cell Dysfunction Research Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Kim
authors.affiliation: Department of Biomedical Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea Cell Dysfunction Research Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.
authors.forename: Youngsup
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Biomedical Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea Cell Dysfunction Research Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.
authors.forename: Sang-Wook
authors.initials: SW
authors.lastname: Kang
authors.affiliation: Department of Oncology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.
authors.forename: Min-Hee
authors.initials: MH
authors.lastname: Rhu
authors.affiliation: Department of Biomedical Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea Cell Dysfunction Research Center, University of Ulsan College of Medicine, Seoul, Republic of Korea ejchang@amc.seoul.kr.
authors.forename: Eun-Ju
authors.initials: EJ
authors.lastname: Chang
mesh_terms: D002097:C-Reactive Protein / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics*; D002465:Cell Movement / Q000235:genetics; D002633:Chemotaxis / Q000187:drug effects; D006801:Humans; D007249:Inflammation / Q000235:genetics* / Q000473:pathology; D008264:Macrophages / Q000378:metabolism / Q000473:pathology; D000683:Serum Amyloid P-Component / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D014945:Wound Healing; D000099153:Pentraxins
chemical_list: D002097:C-Reactive Protein; D000683:Serum Amyloid P-Component; D000099153:Pentraxins
keywords: Gastric cancer; PTX3; inflammation; macrophage