title: Fast monitoring of indoor bioaerosol concentrations with ATP bioluminescence assay using an electrostatic rod-type sampler.
abstract: A culture-based colony counting method is the most widely used analytical technique for monitoring bioaerosols in both indoor and outdoor environments. However, this method requires several days for colony formation. In this study, our goal was fast monitoring (Sampling: 3 min, Detection: < 1 min) of indoor bioaerosol concentrations with ATP bioluminescence assay using a bioaerosol sampler. For this purpose, a novel hand-held electrostatic rod-type sampler (110 mm wide, 115 mm long, and 200 mm tall) was developed and used with a commercial luminometer, which employs the Adenosine triphosphate (ATP) bioluminescence method. The sampler consisted of a wire-rod type charger and a cylindrical collector, and was operated with an applied voltage of 4.5 kV and a sampling flow rate of 150.7 lpm. Its performance was tested using Staphylococcus epidermidis which was aerosolized with an atomizer. Bioaerosol concentrations were measured using ATP bioluminescence method with our sampler and compared with the culture-based method using Andersen cascade impactor under controlled laboratory conditions. Indoor bioaerosol concentrations were also measured using both methods in various indoor environments. A linear correlation was obtained between both methods in lab-tests and field-tests. Our proposed sampler with ATP bioluminescence method may be effective for fast monitoring of indoor bioaerosol concentrations.
authors.affiliation: School of Mechanical Engineering, Yonsei University, Seoul, Republic of Korea.
authors.forename: Ji-Woon
authors.initials: JW
authors.lastname: Park
authors.affiliation: HAE Research and Development Center, LG Electronics, Seoul, Republic of Korea.
authors.forename: Chul Woo
authors.initials: CW
authors.lastname: Park
authors.affiliation: HAE Research and Development Center, LG Electronics, Seoul, Republic of Korea.
authors.forename: Sung Hwa
authors.initials: SH
authors.lastname: Lee
authors.affiliation: School of Mechanical Engineering, Yonsei University, Seoul, Republic of Korea.
authors.forename: Jungho
authors.initials: J
authors.lastname: Hwang
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis*; D000336:Aerosols / Q000032:analysis*; D000391:Air Microbiology; D058491:Bacterial Load / Q000295:instrumentation / Q000379:methods; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D008163:Luminescent Measurements / Q000295:instrumentation*; D013212:Staphylococcus epidermidis / Q000302:isolation & purification
chemical_list: D000336:Aerosols; D000255:Adenosine Triphosphate