title: Expression and localization of interleukin 1 beta and interleukin 1 receptor (type I) in the bovine endometrium and embryo.
abstract: The interleukin-1 (IL1) system likely mediates mammalian embryo-maternal communication. In cattle, we have reported that the uterine fluid of heifers carrying early embryos shows downregulated IL1 beta (IL1B), which could lead to reduced NFkB expression and dampening of maternal innate immune responses. In this work, we assessed the expression of IL 1 beta (IL1B) and its receptor, interleukin 1 receptor type I (IL1R1) in the bovine endometrium and embryos by RT-PCR, immunohistochemistry and Western blot at the time of blastocyst development. Day 8 endometrium, both collected from animals after transfer of day 5 embryos (ET) and sham transferred (ST), showed IL1B and IL1R1 mRNA transcription and protein co-localization. Similarly, day 8 blastocyst, from ET animals and entirely produced in vitro, showed IL1R1 mRNA transcription and IL1B and IL1R1 protein co-localization. IL1B mRNA was detected in the analyzed blastocysts, but at very low levels that precluded its quantification. IL1B and IL1R1 immunostaining was observed in luminal epithelial cells, glandular epithelium and stromal cells. The presence of embryos increased endometrial IL1B protein locally, while no differences regarding IL1R1 protein and IL1B and IL1R1 mRNA were detected. These results suggest that the early preimplantation bovine embryo in the maternal tract might interact with the maternal immune system through the IL1 system. Such a mechanism may allow the embryo to elicit local endometrial responses at early stages, which are required for the development of a receptive endometrium.
authors.affiliation: Centro de Biotecnología Animal-SERIDA, Camino de Rioseco 1225, La Olla-Deva, 33394, Gijón, Asturias, Spain.
authors.forename: Eva
authors.initials: E
authors.lastname: Correia-Álvarez
authors.affiliation: Centro de Biotecnología Animal-SERIDA, Camino de Rioseco 1225, La Olla-Deva, 33394, Gijón, Asturias, Spain.
authors.forename: Enrique
authors.initials: E
authors.lastname: Gómez
authors.affiliation: Centro de Biotecnología Animal-SERIDA, Camino de Rioseco 1225, La Olla-Deva, 33394, Gijón, Asturias, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Martín
authors.affiliation: Centro de Biotecnología Animal-SERIDA, Camino de Rioseco 1225, La Olla-Deva, 33394, Gijón, Asturias, Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: Carrocera
authors.affiliation: Unidad de Coordinación de Trasplantes y Terapia Celular, Hospital Universitario Central de Asturias, Carretera de Rubín s/n, 33011 Oviedo, Spain.
authors.forename: Silvia
authors.initials: S
authors.lastname: Pérez
authors.affiliation: Unidad de Coordinación de Trasplantes y Terapia Celular, Hospital Universitario Central de Asturias, Carretera de Rubín s/n, 33011 Oviedo, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Otero
authors.affiliation: INRA, UMR1198 Biologie du Développement et Reproduction, F-78350 Jouy-en-Josas, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Peynot
authors.affiliation: INRA, UMR1198 Biologie du Développement et Reproduction, F-78350 Jouy-en-Josas, France.
authors.forename: Corinne
authors.initials: C
authors.lastname: Giraud-Delville
authors.affiliation: Centro de Biotecnología Animal-SERIDA, Camino de Rioseco 1225, La Olla-Deva, 33394, Gijón, Asturias, Spain.
authors.forename: José Néstor
authors.initials: JN
authors.lastname: Caamaño
authors.affiliation: INRA, UMR1198 Biologie du Développement et Reproduction, F-78350 Jouy-en-Josas, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Sandra
authors.affiliation: INRA, UMR1198 Biologie du Développement et Reproduction, F-78350 Jouy-en-Josas, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Duranthon
authors.affiliation: Centro de Biotecnología Animal-SERIDA, Camino de Rioseco 1225, La Olla-Deva, 33394, Gijón, Asturias, Spain. Electronic address: mmunoz@serida.org.
authors.forename: Marta
authors.initials: M
authors.lastname: Muñoz
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism*; D002417:Cattle; D047108:Embryonic Development / Q000502:physiology*; D004717:Endometrium / Q000166:cytology / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D053583:Interleukin-1beta / Q000096:biosynthesis*; D017472:Receptors, Interleukin-1 / Q000096:biosynthesis*
chemical_list: D053583:Interleukin-1beta; D017472:Receptors, Interleukin-1
keywords: Blastocyst; Caruncle; Cattle; NFkB; Uterus