title: Target DNA induced switches of DNA polymerase activity.
abstract: A novel concept that target DNA can induce switching of DNA polymerase activity is devised. The method relies on the finding that a DNA aptamer can undergo conformational change upon hybridization with a complementary target DNA, which leads to activation or inactivation of DNA polymerase. This strategy is utilized to identify the presence of target DNA with high levels of sensitivity and selectivity.
authors.affiliation: Department of Chemical and Biomolecular Engineering (BK21+Program), KAIST, Daejeon 305-701, Republic of Korea. hgpark@kaist.ac.kr.
authors.forename: Ki Soo
authors.initials: KS
authors.lastname: Park
authors.forename: Chang Yeol
authors.initials: CY
authors.lastname: Lee
authors.forename: Hyun Gyu
authors.initials: HG
authors.lastname: Park
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D002692:Chlamydia trachomatis; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004269:DNA, Bacterial / Q000737:chemistry / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D016990:Ureaplasma urealyticum
chemical_list: D052157:Aptamers, Nucleotide; D004269:DNA, Bacterial; D004247:DNA; D004259:DNA-Directed DNA Polymerase