title: Detection of Cyclospora in captive chimpanzees and macaques by a quantitative PCR-based mutation scanning approach.
abstract: BACKGROUND
  Cyclospora is a protistan parasite that causes enteritis in several species of animals including humans. The aim of this study was to investigate the presence of Cyclospora in captive non-human primates.
  
  
  METHODS
  A total of 119 faecal samples from Pan troglodytes, Macaca sylvanus, Cercopithecus cephus, Erythrocebus patas, Chlorocebus aethiops and Macaca fascicularis from a wildlife animal rescue center as well as from Macaca fascicularis from an experimental primate research center were tested for the presence of Cyclospora by quantitative real-time PCR (qPCR) and single-strand conformation polymorphism (SSCP) analysis.
  
  
  RESULTS
  Cyclospora was detected in three Pan troglodytes (13.6%) and nine (9.3%) Macaca fascicularis.
  
  
  CONCLUSIONS
  The present study represents the first record of Cyclospora in captive primates in Europe, suggesting the presence of Cyclospora cayetanensis, which is transmissible to humans.
authors.affiliation: Department of Science of Agriculture, Food and Environment, University of Foggia, Foggia, Italy. marianna.marangi@unifg.it.|Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Victoria, Australia. marianna.marangi@unifg.it.
authors.forename: Marianna
authors.initials: M
authors.lastname: Marangi
authors.affiliation: Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Victoria, Australia. anson.koehler@unimelb.edu.au.
authors.forename: Anson V
authors.initials: AV
authors.lastname: Koehler
authors.affiliation: Department of Animal Pathology, Hygiene and Public Health, University of Milan, Milan, Italy. sergio.zanzani@unimi.it.
authors.forename: Sergio A
authors.initials: SA
authors.lastname: Zanzani
authors.affiliation: Department of Animal Pathology, Hygiene and Public Health, University of Milan, Milan, Italy. mariateresa.manfredi@unimi.it.
authors.forename: Maria T
authors.initials: MT
authors.lastname: Manfredi
authors.affiliation: Department of Veterinary Sciences, University of Messina, Messina, Italy. ebrianti@unime.it.
authors.forename: Emanuele
authors.initials: E
authors.lastname: Brianti
authors.affiliation: Department of Science of Agriculture, Food and Environment, University of Foggia, Foggia, Italy. annunziata.giangaspero@unifg.it.
authors.forename: Annunziata
authors.initials: A
authors.lastname: Giangaspero
authors.affiliation: Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Victoria, Australia. robinbg@unimelb.edu.au.
authors.forename: Robin B
authors.initials: RB
authors.lastname: Gasser
mesh_terms: D000818:Animals; D018420:Ape Diseases / Q000175:diagnosis / Q000469:parasitology*; D021744:Cyclospora / Q000235:genetics* / Q000302:isolation & purification; D021866:Cyclosporiasis / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005243:Feces / Q000469:parasitology; D005260:Female; D008251:Macaca / Q000469:parasitology*; D008297:Male; D008992:Monkey Diseases / Q000175:diagnosis / Q000469:parasitology*; D002679:Pan troglodytes / Q000469:parasitology*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D011110:Polymorphism, Genetic; D013045:Species Specificity
chemical_list: D004247:DNA