title: Active hematopoietic hubs in Drosophila adults generate hemocytes and contribute to immune response.
abstract: Blood cell development in Drosophila shares significant similarities with vertebrate. The conservation ranges from biphasic mode of hematopoiesis to signaling molecules crucial for progenitor cell formation, maintenance, and differentiation. Primitive hematopoiesis in Drosophila ensues in embryonic head mesoderm, whereas definitive hematopoiesis happens in larval hematopoietic organ, the lymph gland. This organ, with the onset of pupation, ruptures to release hemocytes into circulation. It is believed that the adult lacks a hematopoietic organ and survives on the contribution of both embryonic and larval hematopoiesis. However, our studies revealed a surge of blood cell development in the dorsal abdominal hemocyte clusters of adult fly. These active hematopoietic hubs are capable of blood cell specification and can respond to bacterial challenges. The presence of progenitors and differentiated hemocytes embedded in a functional network of Laminin A and Pericardin within this hematopoietic hub projects it as a simple version of the vertebrate bone marrow.
authors.affiliation: Developmental Genetics Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research (IISER) Mohali, Knowledge City, Sector 81, Manauli P.O. 140306, India.
authors.forename: Saikat
authors.initials: S
authors.lastname: Ghosh
authors.affiliation: Developmental Genetics Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research (IISER) Mohali, Knowledge City, Sector 81, Manauli P.O. 140306, India.
authors.forename: Arashdeep
authors.initials: A
authors.lastname: Singh
authors.affiliation: Molecular Cell and Developmental Biology Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research (IISER) Mohali, Knowledge City, Sector 81, Manauli P.O. 140306, India.
authors.forename: Sudip
authors.initials: S
authors.lastname: Mandal
authors.affiliation: Developmental Genetics Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research (IISER) Mohali, Knowledge City, Sector 81, Manauli P.O. 140306, India. Electronic address: lolitika@iisermohali.ac.in.
authors.forename: Lolitika
authors.initials: L
authors.lastname: Mandal
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D024141:Collagen Type IV / Q000378:metabolism; D029721:Drosophila Proteins / Q000378:metabolism; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics / Q000276:immunology*; D004625:Embryo, Nonmammalian / Q000166:cytology; D006410:Hematopoiesis / Q000502:physiology*; D006434:Hemocytes / Q000166:cytology / Q000502:physiology*; D007111:Immunity, Cellular / Q000276:immunology*; D007797:Laminin / Q000378:metabolism; D007814:Larva / Q000254:growth & development / Q000276:immunology* / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D024141:Collagen Type IV; D029721:Drosophila Proteins; D007797:Laminin; C585456:Prc protein, Drosophila; C084098:laminin A