title: Biomarker identification and pathway analysis by serum metabolomics of lung cancer.
abstract: Lung cancer is one of the most common causes of cancer death, for which no validated tumor biomarker is sufficiently accurate to be useful for diagnosis. Additionally, the metabolic alterations associated with the disease are unclear. In this study, we investigated the construction, interaction, and pathways of potential lung cancer biomarkers using metabolomics pathway analysis based on the Kyoto Encyclopedia of Genes and Genomes database and the Human Metabolome Database to identify the top altered pathways for analysis and visualization. We constructed a diagnostic model using potential serum biomarkers from patients with lung cancer. We assessed their specificity and sensitivity according to the area under the curve of the receiver operator characteristic (ROC) curves, which could be used to distinguish patients with lung cancer from normal subjects. The pathway analysis indicated that sphingolipid metabolism was the top altered pathway in lung cancer. ROC curve analysis indicated that glycerophospho-N-arachidonoyl ethanolamine (GpAEA) and sphingosine were potential sensitive and specific biomarkers for lung cancer diagnosis and prognosis. Compared with the traditional lung cancer diagnostic biomarkers carcinoembryonic antigen and cytokeratin 19 fragment, GpAEA and sphingosine were as good or more appropriate for detecting lung cancer. We report our identification of potential metabolic diagnostic and prognostic biomarkers of lung cancer and clarify the metabolic alterations in lung cancer.
authors.affiliation: Huzhou Key Laboratory of Molecular Medicine, Huzhou Central Hospital, Huzhou, Zhejiang 313000, China.
authors.forename: Yingrong
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Huzhou Key Laboratory of Molecular Medicine, Huzhou Central Hospital, Huzhou, Zhejiang 313000, China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Ma
authors.affiliation: Huzhou Key Laboratory of Molecular Medicine, Huzhou Central Hospital, Huzhou, Zhejiang 313000, China.
authors.forename: Lishan
authors.initials: L
authors.lastname: Min
authors.affiliation: Cardiothoracic Surgery, Huzhou Central Hospital, Huzhou, Zhejiang 313000, China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Li
authors.affiliation: Respiratory Medicine, Huzhou Central Hospital, Huzhou, Zhejiang 313000, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: Huzhou Key Laboratory of Molecular Medicine, Huzhou Central Hospital, Huzhou, Zhejiang 313000, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhong
authors.affiliation: Huzhou Key Laboratory of Molecular Medicine, Huzhou Central Hospital, Huzhou, Zhejiang 313000, China ; School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China.
authors.forename: Licheng
authors.initials: L
authors.lastname: Dai
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood*; D030562:Databases, Protein; D063388:Endocannabinoids / Q000097:blood*; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood* / Q000235:genetics / Q000473:pathology; D008297:Male; D053858:Metabolic Networks and Pathways / Q000235:genetics; D055442:Metabolome / Q000235:genetics; D008875:Middle Aged; D011379:Prognosis; D013110:Sphingosine / Q000097:blood*
chemical_list: D014408:Biomarkers, Tumor; D063388:Endocannabinoids; C561029:arachidonoyl-2 chloroethanolamine; D013110:Sphingosine