title: Kank1 reexpression induced by 5-Aza-2'-deoxycytidine suppresses nasopharyngeal carcinoma cell proliferation and promotes apoptosis.
abstract: Kank1, which was first described as a potential tumor suppressor for renal cell carcinoma (RCC), mapped to 9p24.3 and encoded an ankyrin-repeat domain-containing protein. Its frequent deletion was found to be associated with several human malignant tumors, cerebral palsy, and neuronal and developmental diseases. However, its functional role in nasopharyngeal cancer (NPC) was still unknown. In the present study, we found that Kank1 expression was down-regulated in NPC cells than in human nasopharyngeal epithelial cell line NP69 and demethylating agent 5-aza-2'-deoxycytidine (5-aza-CdR) could improve its mRNA and protein expression level. Further studies demonstrated that DNA methylation might be the mainly cause for Kank1 decreased expression and restored Kank1 expression mediated by 5-aza-CdR played a key role in suppressing NPC cells growth and inducing its apoptosis. Our primary results revealed new function of Kank1 for NPC and implied that epigenetic regulation especially demethylation may have a potential value for NPC treatment.
authors.affiliation: Department of Cardiothoracic Surgery, Xiangya Hospital, Central South University Changsha 410008, Hunan, China.
authors.forename: Fan-Yan
authors.initials: FY
authors.lastname: Luo
authors.affiliation: The Central Hospital of Wuhan Wuhan 430014, Hubei, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Xiao
authors.affiliation: Department of Cardiothoracic Surgery, Xiangya Hospital, Central South University Changsha 410008, Hunan, China.
authors.forename: Zi-Hou
authors.initials: ZH
authors.lastname: Liu
authors.affiliation: Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University Changsha 410008, Hunan, China.
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Zhang
authors.affiliation: Research Center of Carcinogene and Target Therapy, Xiangya Hospital, Central South University Changsha 410008, Hunan, China.
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Xiao
authors.affiliation: Institute of Medical Sciences, Xiangya Hospital, Central South University Changsha 410008, Hunan, China.
authors.forename: Can-E
authors.initials: CE
authors.lastname: Tang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D001374:Azacitidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D015153:Blotting, Western; D002277:Carcinoma; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects / Q000235:genetics; D003598:Cytoskeletal Proteins; D019175:DNA Methylation; D000077209:Decitabine; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000473:pathology*; D060888:Real-Time Polymerase Chain Reaction; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D000964:Antimetabolites, Antineoplastic; D003598:Cytoskeletal Proteins; C467739:KANK1 protein, human; D025521:Tumor Suppressor Proteins; D000077209:Decitabine; D001374:Azacitidine
keywords: 5-aza-CdR; Kank1; apoptosis; methylation; nasopharyngeal carcinoma; proliferation