title: Decreased Expression of Inhibitor of DNA-binding (Id) Proteins and Vascular Endothelial Growth Factor and Increased Apoptosis in Ovarian Aging.
abstract: This study examined the expression of inhibitor of DNA-binding (Id) proteins and vascular endothelial growth factor (VEGF) in the ovary according to female age using a mice model as the first step in investigating the potential role of Ids and VEGF in ovarian aging. C57BL inbred female mice of three age groups (6-9, 14-16, and 23-26 weeks) were injected with 5 IU pregnant mare's serum gonadotropin (PMSG) in order to synchronize the estrus cycle. After 48 h, ovarian expression of Ids and VEGF was analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR), western blot and immunohistochemistry. Ovarian apoptosis was examined by ovarian expression of Bcl-2 and Bcl-xL. Expression of Id-1 and VEGF was decreased with advancing female age, but not Id-2, Id-3, and Id-4. In particular, their expressions were significantly decreased in aged mice of 23-26 weeks compared with the young mice of 6-9 weeks (p < 0.05). In contrast, ovarian apoptosis was greatly increased in the aged mice compared to the young mice. This result suggests that Id-1 may have an implicated role in ovarian aging by associating with VEGF.
authors.affiliation: Center for Reproductive Medicine, Good Moonhwa Hospital, Busan 601-803, Korea.
authors.forename: Min Jung
authors.initials: MJ
authors.lastname: Park
authors.affiliation: Center for Reproductive Medicine, Good Moonhwa Hospital, Busan 601-803, Korea.
authors.forename: Sea Hee
authors.initials: SH
authors.lastname: Park
authors.affiliation: Department of Obstetrics and Gynecology, Good Moonhwa Hospital, Busan 601-803, Korea.
authors.forename: Sung Eun
authors.initials: SE
authors.lastname: Moon
authors.affiliation: Department of Obstetrics and Gynecology, Good Moonhwa Hospital, Busan 601-803, Korea.
authors.forename: Ja Seong
authors.initials: JS
authors.lastname: Koo
authors.affiliation: Department of Obstetrics and Gynecology, Good Moonhwa Hospital, Busan 601-803, Korea.
authors.forename: Hwa Sook
authors.initials: HS
authors.lastname: Moon
authors.affiliation: Center for Reproductive Medicine, Good Moonhwa Hospital, Busan 601-803, Korea.
authors.forename: Bo Sun
authors.initials: BS
authors.lastname: Joo
keywords: Apoptosis; Id proteins; Ovarian aging; VEGF