title: Involvement of leucocyte/endothelial cell interactions in anorexia nervosa.
abstract: BACKGROUND
  Anorexia nervosa is a common psychiatric disorder in adolescence and is related to cardiovascular complications. Our aim was to study the effect of anorexia nervosa on metabolic parameters, leucocyte-endothelium interactions, adhesion molecules and proinflammatory cytokines.
  
  
  METHODS
  This multicentre, cross-sectional, case-control study employed a population of 24 anorexic female patients and 36 controls. We evaluated anthropometric and metabolic parameters, interactions between leucocytes polymorphonuclear neutrophils (PMN) and human umbilical vein endothelial cells (HUVEC), proinflammatory cytokines such as tumour necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) and soluble cellular adhesion molecules (CAMs) including E-selectin, vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1).
  
  
  RESULTS
  Anorexia nervosa was related to a decrease in weight, body mass index, waist circumference, systolic blood pressure, glucose, insulin and HOMA-IR, and an increase in HDL cholesterol. These effects disappeared after adjusting for BMI. Anorexia nervosa induced a decrease in PMN rolling velocity and an increase in PMN rolling flux and PMN adhesion. Increases in IL-6 and TNF-α and adhesion molecule VCAM-1 were also observed.
  
  
  CONCLUSIONS
  This study supports the hypothesis of an association between anorexia nervosa, inflammation and the induction of leucocyte-endothelium interactions. These findings may explain, in part at least, the increased risk of vascular disease among patients with anorexia nervosa.
authors.affiliation: Foundation for the Promotion of Healthcare and Biomedical Research in the Valencian Community (FISABIO), Valencia, Spain.|Service of Endocrinology, University Hospital Doctor Peset, Valencia, Spain.|Institute of Health Research INCLIVA, University of Valencia, Valencia, Spain.|Department of Physiology, University of Valencia, Valencia, Spain.
authors.forename: Víctor M
authors.initials: VM
authors.lastname: Víctor
authors.affiliation: Foundation for the Promotion of Healthcare and Biomedical Research in the Valencian Community (FISABIO), Valencia, Spain.|Service of Endocrinology, University Hospital Doctor Peset, Valencia, Spain.|Institute of Health Research INCLIVA, University of Valencia, Valencia, Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: Rovira-Llopis
authors.affiliation: Psychiatry Service, University Hospital La Fe, Valencia, Spain.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Saiz-Alarcón
authors.affiliation: Psychiatry Service, University Hospital La Fe, Valencia, Spain.
authors.forename: Maria C
authors.initials: MC
authors.lastname: Sangüesa
authors.affiliation: Psychiatry Service, University Hospital La Fe, Valencia, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Rojo-Bofill
authors.affiliation: Foundation for the Promotion of Healthcare and Biomedical Research in the Valencian Community (FISABIO), Valencia, Spain.|Service of Endocrinology, University Hospital Doctor Peset, Valencia, Spain.|Institute of Health Research INCLIVA, University of Valencia, Valencia, Spain.
authors.forename: Celia
authors.initials: C
authors.lastname: Bañuls
authors.affiliation: Department of Pharmacology and CIBER CB06/04/0071 Research Group, CIBER Hepatic and Digestive Diseases, University of Valencia, Valencia, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: de Pablo
authors.affiliation: Department of Pharmacology and CIBER CB06/04/0071 Research Group, CIBER Hepatic and Digestive Diseases, University of Valencia, Valencia, Spain.|Fundacion General de la Universidad de Valencia, Valencia, Spain.
authors.forename: Ángeles
authors.initials: Á
authors.lastname: Álvarez
authors.affiliation: Psychiatry Service, University Hospital La Fe, Valencia, Spain.|Research Group CIBER CB/06/02/0045 CIBER actions - Epidemiology and Public Health, University of Valencia, Valencia, Spain.|Department of Medicine, University of Valencia, Valencia, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Rojo
authors.affiliation: Foundation for the Promotion of Healthcare and Biomedical Research in the Valencian Community (FISABIO), Valencia, Spain.|Service of Endocrinology, University Hospital Doctor Peset, Valencia, Spain.|Institute of Health Research INCLIVA, University of Valencia, Valencia, Spain.
authors.forename: Milagros
authors.initials: M
authors.lastname: Rocha
authors.affiliation: Foundation for the Promotion of Healthcare and Biomedical Research in the Valencian Community (FISABIO), Valencia, Spain.|Service of Endocrinology, University Hospital Doctor Peset, Valencia, Spain.|Institute of Health Research INCLIVA, University of Valencia, Valencia, Spain.|Department of Medicine, University of Valencia, Valencia, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Hernández-Mijares
mesh_terms: D000293:Adolescent; D000328:Adult; D000856:Anorexia Nervosa / Q000473:pathology*; D015992:Body Mass Index; D016022:Case-Control Studies; D015815:Cell Adhesion Molecules / Q000378:metabolism; D003430:Cross-Sectional Studies; D016207:Cytokines / Q000378:metabolism; D042783:Endothelial Cells / Q000502:physiology*; D004730:Endothelium, Vascular / Q000378:metabolism / Q000473:pathology; D005260:Female; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D007249:Inflammation / Q000378:metabolism / Q000473:pathology; D007963:Leukocytes, Mononuclear / Q000502:physiology*; D055815:Young Adult
chemical_list: D015815:Cell Adhesion Molecules; D016207:Cytokines
keywords: Adhesion; anorexia; cytokines; leucocytes/endothelium interaction; lipid profile