title: Development of a ubiquitously transferrable silver-nanoparticle-loaded polymer nanosheet as an antimicrobial coating.
abstract: Ultra-thin polymer films (nanosheets) fabricated by a layer-by-layer (LbL) method possess unique properties such as high flexibility, adhesive strength, and transparency, and can be peeled off from a substrate and attached to various surfaces via a water-soluble supporting film. Therefore, flexible and transferrable LbL nanosheets are convenient tools as coating materials. Here, we fabricated a novel antimicrobial coating material by embedding silver nanoparticles (AgNPs) in an LbL nanosheet composed of layers of chitosan and sodium alginate (Ag-LbL nanosheet) by means of a photo-reduction method. Optimizing the amount of irradiated energy applied led to robust antimicrobial efficacy against methicillin-resistant Staphylococcus aureus (MRSA), sufficient to meet ISO standards (ISO 22196), while maintaining the flexibility and adhesive potency of the LbL nanosheet. Thus, the Ag-LbL nanosheet is a promising coating material that can provide antimicrobial efficacy to various surfaces.
authors.affiliation: Department of Life Science and Medical Bio-Science, Graduate School of Advanced Science and Engineering, Waseda University, Tokyo, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Ito
authors.affiliation: Department of Life Science and Medical Bio-Science, Graduate School of Advanced Science and Engineering, Waseda University, Tokyo, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Saito
authors.affiliation: Department of Life Science and Medical Bio-Science, Graduate School of Advanced Science and Engineering, Waseda University, Tokyo, Japan.
authors.forename: Toshinori
authors.initials: T
authors.lastname: Fujie
authors.affiliation: Division of Traumatology, Research institute, National Defense Medical College, Saitama, Japan.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Miyazaki
authors.affiliation: Department of Immunology and Microbiology, National Defense Medical College, Saitama, Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Kinoshita
authors.affiliation: Division of Traumatology, Research institute, National Defense Medical College, Saitama, Japan.
authors.forename: Daizoh
authors.initials: D
authors.lastname: Saitoh
authors.affiliation: Project Research Institutes, Comprehensive Research Organization, Waseda University, Tokyo, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Ohtsubo
authors.affiliation: Department of Life Science and Medical Bio-Science, Graduate School of Advanced Science and Engineering, Waseda University, Tokyo, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Takeoka
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D008567:Membranes, Artificial; D053768:Metal Nanoparticles / Q000737:chemistry*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000254:growth & development*; D012834:Silver / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000890:Anti-Infective Agents; D008567:Membranes, Artificial; D012834:Silver
keywords: antimicrobial; bacterial adherence; coating; in vitro; polymer