title: Cytotoxicity of Endogenous Lipids N-acyl Dopamines and their Possible Metabolic Derivatives for Human Cancer Cell Lines of Different Histological Origin.
abstract: OBJECTIVE
  Dopamine amides of long chain fatty acids are a family of endogenous mammalian lipids with an unknown function; they are anti-proliferative for the C6 glioblastoma cell line. To assess their possible anti-cancer activity we evaluated their cytotoxicity for a set of cancer cell lines.
  
  
  METHODS
  Anti-proliferative and cytotoxic actions of these substances were evaluated in HOS, IMR-32, MCF-7, Namalwa, K-562 and HEK 293 cell lines (18 h incubation time) using MTT and lactate dehydrogenase (LDH) tests, accordingly.
  
  
  RESULTS
  All N-acyl dopamines (NADA) induced cell death in all cell lines tested with a 50% lethal dose (LD50) in the range of 0.5-80 μM, except for HEK-293. For HEK-293 only N-arachidonoyl epinephrine demonstrated an LD50 below 100 μM.
  
  
  CONCLUSIONS
  According to the structure-activity relationship, N-acyl dopamines with an intact catechol group and a non-modified hydrophobic fatty acid residue are cytotoxic to cancer cell lines of various histological origins.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia akimovmike@yandex.ru.
authors.forename: Mikhail G
authors.initials: MG
authors.lastname: Akimov
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Natalia M
authors.initials: NM
authors.lastname: Gretskaya
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Galina N
authors.initials: GN
authors.lastname: Zinchenko
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Vladimir V
authors.initials: VV
authors.lastname: Bezuglov
mesh_terms: D000818:Animals; D004298:Dopamine / Q000031:analogs & derivatives / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D005909:Glioblastoma / Q000378:metabolism* / Q000473:pathology; D057809:HEK293 Cells; D006801:Humans; D008055:Lipids; D061986:MCF-7 Cells; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology; D013329:Structure-Activity Relationship
chemical_list: D005227:Fatty Acids; D008055:Lipids; D004298:Dopamine
keywords: N-acyl dopamine; cancer; cytotoxicity; metabolites; vanilloids