title: MiR-361-5p inhibits colorectal and gastric cancer growth and metastasis by targeting staphylococcal nuclease domain containing-1.
abstract: MicroRNAs (miRs) function as key regulators of gene expression and their deregulation is associated with the carcinogenesis of various cancers. In the present study, we investigated the biological role and mechanism of miR-361-5p in colorectal carcinoma (CRC) and gastric cancer (GC). We showed that microRNA-361-5p (miR-361-5p) was down-regulated in CRC and GC in comparison to the controls. Meanwhile, the expression levels of miR-361-5p negatively correlated with lung metastasis and prognosis in clinical CRC patients. Overexpression of miR-361-5p markedly suppressed proliferation, migration and invasion of cancer cells. Additionally, this phenotype could be partially rescued by the ectopic expression of staphylococcal nuclease domain containing-1 (SND1). SND1 was identified as a target of miR-361-5p using bioinformatics analysis and in vitro luciferase reporter assays. In turn, SND1 bound to pre-miR-361-5p and suppressed the expression of miR-361-5p, thus exerting a feedback loop. Most interestingly, in vivo studies showed that restoration of miR-361-5p significantly inhibited tumor growth and especially the lung metastasis in nude mice. Therefore, it could be concluded that miR-361-5p functions as a tumor-suppressive miRNA through directly binding to SND1, highlighting its potential as a novel agent for the treatment of patients with CRC and GC.
authors.affiliation: Department of Endoscopy, The Affiliated Cancer Hospital, Harbin Medical University, Harbin, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Ma
authors.affiliation: Department of Endoscopy, The Affiliated Cancer Hospital, Harbin Medical University, Harbin, China.|Department of Gastrointestinal Surgery, The Affiliated Cancer Hospital, Harbin Medical University, Harbin, China.
authors.forename: Hongjiang
authors.initials: H
authors.lastname: Song
authors.affiliation: Department of General Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Baoliang
authors.initials: B
authors.lastname: Guo
authors.affiliation: Department of Gastrointestinal Surgery, The Affiliated Cancer Hospital, Harbin Medical University, Harbin, China.
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, Central Hospital of Jiuzhen, China.
authors.forename: Yasheng
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Department of Gastroenterology, The First Hospital of Longyan, Fujian Medical University, Longyan, China.
authors.forename: Chengchun
authors.initials: C
authors.lastname: Lin
authors.affiliation: Department of Pathology, Hongqi Hospital, Mudanjiang Medical University, Mudanjiang, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Pathology, Hongqi Hospital, Mudanjiang Medical University, Mudanjiang, China.
authors.forename: Guijie
authors.initials: G
authors.lastname: Guan
authors.affiliation: Department of Digestive Disease, Hongqi Hospital, Mudanjiang Medical University, Mudanjiang, China.
authors.forename: Ruihua
authors.initials: R
authors.lastname: Sha
authors.affiliation: Department of General Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Qingxin
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Department of Ultrasound of Obstetrics and Gynecology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Dejun
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Medical Imaging, The Affiliated Cancer Hospital of Harbin Medical University, Harbin, China.
authors.forename: Xinglu
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Pathology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.affiliation: The Second Hospital of Longyan, Fujian Medical University, Longyan, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Qiu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D004720:Endonucleases; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D064593:Heterografts; D006801:Humans; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D017403:In Situ Hybridization; D053208:Kaplan-Meier Estimate; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D018613:Microscopy, Confocal; D009361:Neoplasm Invasiveness / Q000235:genetics; D009362:Neoplasm Metastasis; D009687:Nuclear Proteins / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D014162:Transfection
chemical_list: C575475:MIRN361 microRNA, human; D035683:MicroRNAs; D009687:Nuclear Proteins; D004720:Endonucleases; C095221:SND1 protein, human
keywords: RNA-induced silencing complex (RISC); Tudor staphylococcal nuclease 1; colorectal carcinoma; gastric cancer; miR-361-5p