title: Kinetics and functional effectiveness of nisin loaded antimicrobial packaging film based on chitosan/poly(vinyl alcohol).
abstract: The aim of this study was to evaluate the kinetics and functional effectiveness of Nisin loaded chitosan/poly(vinyl alcohol) (Nisin-CS/PVA) as an antibacterial packaging film. The films were prepared by coating method and Staphylococcus aureus (S. aureus, ATCC6538) was used as test bacterium. The intermolecular hydrogen bonds between CS and PVA molecules were confirmed. The elasticity of films was significantly improved by the incorporation of PVA, and the film could also bear a relative high tensile strength at 26.7 MPa for CS/PVA=1/1. As CS/PVA ratio decreased, the water vapor permeability (WVP) decreased and reached its minimum value 0.983 × 10(-10)gm(-1)s(-1) at CS/PVA=1/1, meanwhile, oxygen permeability (OP) increased but still lower than 0.91 cm(3) μm m(-2)d(-1)kPa(-1) for CS/PVA=1/1 as the CS/PVA ratio was above 1:1. The initial diffusion of nisin (Mt/M ∞ < 2/3) from CS/PVA film could be well described by the Fickian diffusion equation. Owing to the positively charged nisin at pH below isoelectric point (pI, 8.8) and its increasing dissolubility in water as the pH reduced, the diffusion of nisin from the films strongly depended on pH and ionic strength besides CS/PVA ratio and temperature. Moreover, the thermodynamic parameters suggested the spontaneous and endothermic diffusion of nisin from the films. The resulting data can provide some valuable information for the design of film in structure and ingredient.
authors.affiliation: School of Chemistry and Chemical Technology, Hefei University of Technology, Hefei 230009, Anhui, People's Republic of China; Anhui Institute of Agro-Products Intensive Processing Technology, Hefei 230009, Anhui, People's Republic of China. Electronic address: hlwang@hfut.edu.cn.
authors.forename: Hualin
authors.initials: H
authors.lastname: Wang
authors.affiliation: School of Chemistry and Chemical Technology, Hefei University of Technology, Hefei 230009, Anhui, People's Republic of China.
authors.forename: Ru
authors.initials: R
authors.lastname: Zhang
authors.affiliation: School of Chemistry and Chemical Technology, Hefei University of Technology, Hefei 230009, Anhui, People's Republic of China.
authors.forename: Heng
authors.initials: H
authors.lastname: Zhang
authors.affiliation: School of Chemistry and Chemical Technology, Hefei University of Technology, Hefei 230009, Anhui, People's Republic of China.
authors.forename: Suwei
authors.initials: S
authors.lastname: Jiang
authors.affiliation: School of Chemistry and Chemical Technology, Hefei University of Technology, Hefei 230009, Anhui, People's Republic of China.
authors.forename: Huan
authors.initials: H
authors.lastname: Liu
authors.affiliation: School of Chemistry and Chemical Technology, Hefei University of Technology, Hefei 230009, Anhui, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Sun
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, Anhui, People's Republic of China; Anhui Institute of Agro-Products Intensive Processing Technology, Hefei 230009, Anhui, People's Republic of China.
authors.forename: Shaotong
authors.initials: S
authors.lastname: Jiang
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology*; D004058:Diffusion; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D009561:Nisin / Q000737:chemistry / Q000494:pharmacology*; D011142:Polyvinyl Alcohol / Q000737:chemistry / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects*; D013816:Thermodynamics
chemical_list: D000900:Anti-Bacterial Agents; D009561:Nisin; D011142:Polyvinyl Alcohol; D048271:Chitosan
keywords: Antimicrobial packaging film; Functional effectiveness; Kinetics; Nisin–chitosan/poly(vinyl alcohol); Thermodynamics