title: Erythrocyte Protoporphyrin Fluorescence as a Biomarker to Monitor the Anticancer Effect of Semecarpus Anacardium in DMBA Induced Mammary Carcinoma Rat Model.
abstract: Endogenous fluorescence has been proposed as a means of aiding the diagnosis of various malignancies. It has been suggested that erythrocytes may be the carriers of fluorophors that accumulate in cancer tissue and may be useful in the diagnosis and treatment of malignancies. Hence, the present study was designed to explore the spectrofluorimetric analysis of blood components as a marker for the analysis of mammary carcinoma treatment and also to bring about the protective effect of the drug Semecarpus anacardium on oxidative stress mediated damage of erythrocytes. Fluorescence spectra of the blood components were studied and also the level of lipid per oxides and antioxidant enzymes status in erythrocytes were determined in DMBA induced mammary carcinoma rats treated with Semecarpus anacardium Linn nut milk extract. Fluorescence emission spectroscopy of blood components are altered under cancer conditions and the drug effectively ameliorated these alterations in mammary carcinoma induced rats. The drug also effectively reduced the oxidative stress induced erythrocyte damage thereby restoring the erythrocytes antioxidant status. These results suggest that erythrocytes may be the carriers of fluorophors that accumulate in cancer tissue and hence acts as new biomarkers for the diagnosis and treatment.
authors.affiliation: Dr ALM P-G IBMS, University of Madras, Chennai, Tamil Nadu, India.
authors.forename: Haseena Banu Hedayathullah
authors.initials: HB
authors.lastname: Khan
authors.forename: S
authors.initials: S
authors.lastname: Vani
authors.forename: Shanthi
authors.initials: S
authors.lastname: Palanivelu
authors.forename: Sachdanandam
authors.initials: S
authors.lastname: Panchanadham
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis*; D002273:Carcinogens / Q000633:toxicity; D004195:Disease Models, Animal; D004912:Erythrocytes / Q000187:drug effects / Q000473:pathology*; D005260:Female; D015227:Lipid Peroxidation / Q000187:drug effects; D008325:Mammary Neoplasms, Experimental / Q000188:drug therapy / Q000473:pathology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011524:Protoporphyrins / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D031023:Semecarpus / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D002273:Carcinogens; D010936:Plant Extracts; D011524:Protoporphyrins; D015127:9,10-Dimethyl-1,2-benzanthracene; C028025:protoporphyrin IX