title: Hypermethylated Epidermal growth factor receptor (EGFR) promoter is associated with gastric cancer.
abstract: Epidermal growth factor receptor (EGFR) is a member of the receptor tyrosine kinases ErbB family and it is found to be overexpressed in gastric cancer. However, the mechanism of the regulation of the EGFR expression is still unknown. We used the Sequenom EpiTYPER assay to detect the methylation status of the EGFR promoter in normal and tumour tissues of 30 patients with gastric cancer. We also carried out quantitative real time PCR (qPCR) to detect the expression level of EGFR in our 30 patients. Notably, increased methylation level at EGFR promoter was found in tumour tissues than the corresponding adjacent noncancerous. In both Region I DMR and Region II DMR detected in our study, tumor tissues were significantly hypermethylated (P=2.7743E-10 and 2.1703E-05, respectively). Region I_⊿CpG_2 was also found to be associated with the presence of distant metastasis (P=0.0323). Furthermore, the results showed a strongly significant association between the relative EGFR expression and the EGFR methylation changes in both Region I and Region II (P=0.0004 and 0.0001, respectively). Our findings help to indicate the hypermethylation at EGFR promoter in gastric cancer and it could be a potential epigenetic biomarker for gastric cancer status and progression.
authors.affiliation: Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, PR China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Weng
authors.affiliation: Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, PR China.
authors.forename: Junyi
authors.initials: J
authors.lastname: Ye
authors.affiliation: Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, PR China.
authors.forename: Mengyuan
authors.initials: M
authors.lastname: Kan
authors.affiliation: Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, PR China.
authors.forename: Fatao
authors.initials: F
authors.lastname: Liu
authors.affiliation: Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, PR China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Radiation Oncology, Fudan University Shanghai Cancer Center, 270 Dongan Road, Shanghai 200032, PR China.
authors.forename: Jiaying
authors.initials: J
authors.lastname: Deng
authors.affiliation: Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, PR China.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Tan
authors.affiliation: 1] Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, PR China [2] Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, PR China [3] Bio-X Center, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders, Ministry of Education, Shanghai Jiao Tong University, Shanghai 200030, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: He
authors.affiliation: 1] Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, PR China [2] Key Laboratory of Molecular Medicine, Ministry of Education, Department of Biochemistry and Molecular Biology, Fudan University Shanghai Medical College, Shanghai 200032, PR China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019175:DNA Methylation / Q000235:genetics*; D066246:ErbB Receptors / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D011401:Promoter Regions, Genetic; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: C512478:EGFR protein, human; D066246:ErbB Receptors