title: Caffeic acid attenuates rat liver reperfusion injury through sirtuin 3-dependent regulation of mitochondrial respiratory chain.
abstract: Sirtuin 3 (Sirt3) plays critical roles in regulating mitochondrial oxidative metabolism. However, whether Sirt3 is involved in liver ischemia and reperfusion (I/R) injury remains elusive. Caffeic acid (CA) is a natural antioxidant derived from Salvia miltiorrhiza. Whether CA protects against liver I/R injury through regulating Sirt3 and the mitochondrial respiratory chain (MRC) is unclear. This study investigated the effect of CA on liver I/R injury, microcirculatory disturbance, and potential mechanisms, particularly focusing on Sirt3-dependent MRC. Liver I/R of male Sprague-Dawley rats was established by occlusion of portal area vessels for 30 min followed by 120 min of reperfusion. CA (15 mg/kg/h) was continuously infused via the femoral vein starting 30 min before ischemia. After I/R, Sirt3 expression, and MRC activity decreased, acetylation of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9 and succinate dehydrogenase complex, subunit A, flavoprotein variant provoked, and the liver microcirculatory disturbance and injury were observed. Treatment with CA attenuated liver injury, inhibited Sirt3 down-expression, and up-regulated MRC activity. CA attenuated rat liver microcirculatory disturbance and oxidative injury through regulation of Sirt3 and the mitochondrial respiratory chain.
authors.affiliation: Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, China; Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China; Key Laboratory of Stasis and Phlegm of State Administration of Traditional Chinese Medicine, Beijing, China.
authors.forename: Hong-Na
authors.initials: HN
authors.lastname: Mu
authors.affiliation: Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China; Key Laboratory of Stasis and Phlegm of State Administration of Traditional Chinese Medicine, Beijing, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Li
authors.affiliation: Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China; Key Laboratory of Stasis and Phlegm of State Administration of Traditional Chinese Medicine, Beijing, China.
authors.forename: Chun-Shui
authors.initials: CS
authors.lastname: Pan
authors.affiliation: Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China; Key Laboratory of Stasis and Phlegm of State Administration of Traditional Chinese Medicine, Beijing, China.
authors.forename: Yu-Ying
authors.initials: YY
authors.lastname: Liu
authors.affiliation: Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China; Key Laboratory of Stasis and Phlegm of State Administration of Traditional Chinese Medicine, Beijing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yan
authors.affiliation: Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China; Key Laboratory of Stasis and Phlegm of State Administration of Traditional Chinese Medicine, Beijing, China.
authors.forename: Bai-He
authors.initials: BH
authors.lastname: Hu
authors.affiliation: Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China; Key Laboratory of Stasis and Phlegm of State Administration of Traditional Chinese Medicine, Beijing, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Sun
authors.affiliation: Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China; Key Laboratory of Stasis and Phlegm of State Administration of Traditional Chinese Medicine, Beijing, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Chang
authors.affiliation: Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China; Key Laboratory of Stasis and Phlegm of State Administration of Traditional Chinese Medicine, Beijing, China.
authors.forename: Xin-Rong
authors.initials: XR
authors.lastname: Zhao
authors.affiliation: Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China; Key Laboratory of Stasis and Phlegm of State Administration of Traditional Chinese Medicine, Beijing, China.
authors.forename: Jing-Yu
authors.initials: JY
authors.lastname: Fan
authors.affiliation: Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, China; Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China; Key Laboratory of Stasis and Phlegm of State Administration of Traditional Chinese Medicine, Beijing, China. Electronic address: hanjingyan@bjmu.edu.cn.
authors.forename: Jing-Yan
authors.initials: JY
authors.lastname: Han
mesh_terms: D000818:Animals; D002109:Caffeic Acids / Q000494:pharmacology*; D004579:Electron Transport / Q000187:drug effects*; D008099:Liver / Q000098:blood supply / Q000187:drug effects*; D008928:Mitochondria / Q000378:metabolism*; D051381:Rats; D015427:Reperfusion Injury / Q000517:prevention & control*; D056566:Sirtuin 3 / Q000378:metabolism*; D020349:Surface Plasmon Resonance
chemical_list: D002109:Caffeic Acids; D056566:Sirtuin 3; C040048:caffeic acid
keywords: Apoptosis; Leukocyte adherence; Liver microcirculatory disturbance; Salvia miltiorrhiza; Superoxide anion