title: Modifications of collagen-based biomaterials with immobilized growth factors or peptides.
abstract: In order to provide an instructive microenvironment to facilitate cellular behaviors and tissue regeneration, biomaterials can be modified by immobilizing growth factors or peptides. We describe here our procedure for modification of collagen-based biomaterials, both porous scaffolds and hydrogel systems, with growth factors or peptides by covalent immobilization. Characterizations of the modified biomaterials (immobilization efficiency, release profile, morphology, mechanical strength, and rheology) and in vitro testing with cells are also discussed.
authors.affiliation: Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON, Canada; Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada.
authors.forename: Yun
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada.
authors.forename: Lewis A
authors.initials: LA
authors.lastname: Reis
authors.affiliation: Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON, Canada.
authors.forename: Yimu
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON, Canada; Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada; Toronto General Research Institute, University Health Network and IBBME, University of Toronto, Toronto, ON, Canada. Electronic address: m.radisic@utoronto.ca.
authors.forename: Milica
authors.initials: M
authors.lastname: Radisic
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D018929:Cell Culture Techniques / Q000379:methods; D048271:Chitosan / Q000737:chemistry; D003094:Collagen / Q000737:chemistry*; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D055767:Immobilized Proteins / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D036341:Intercellular Signaling Peptides and Proteins / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D008422:Materials Testing; D016062:Porosity; D044968:Regenerative Medicine / Q000379:methods; D012212:Rheology; D013718:Tensile Strength; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D055767:Immobilized Proteins; D036341:Intercellular Signaling Peptides and Proteins; D003094:Collagen; D048271:Chitosan
keywords: Basic fibroblast growth factor; Chitosan; Collagen; Growth factor; Hydrogel; Peptide; Porous scaffold; QHREDGS; Vascular endothelial growth factor