title: Partial protection and abomasal cytokine expression in sheep experimentally infected with Haemonchus contortus and pre-treated with Taenia hydatigena vesicular concentrate.
abstract: The abomasal expression of IL-2, IL-4, IL-6, IL-10 and IFNγ in lambs experimentally infected with Haemonchus contortus and its relationship to protection induced by a Taenia hydatigena larvae vesicular concentrate (ThLVC) were evaluated. The lambs that were only infected with H. contortus larvae showed a worm burden greater (p<0.05) than the lambs that received ThLVC prior to infection. Moreover, the lambs that received ThLVC showed a greater (p<0.05) number of blood eosinophils than the lambs that did not receive the ThLVC. In general, the lambs that received ThLVC prior to infection had a greater amount of eosinophils and mast cells and higher in situ expression of IFNγ, IL-2, IL-4, IL-6 and IL-10 in the abomasal wall than the lambs that were infected with H. contortus only or that received ThLVC (p<0.05) only. A higher expression of IL-2 and IFNγ in the submucosa compared to the abomasal mucosa and a higher expression of IL-4 in the abomasal mucosa compared to the submucosa was observed (p<0.05). These results suggest that there is a Th1 type response in the abomasal submucosa and a Th2 type response in in the abomasal mucosa. The amount of eosinophils and mast cells and the in situ expression of IFNγ, IL-2, IL-4 and IL-6 in the abomasal walls were negatively correlated with the worm burden (p<0.05). These results suggest that ThLVC is a non-specific immune stimulator for the abomasal immune response, and it is likely that the protection observed is the result of this effect.
authors.affiliation: Programa de Doctorado en Ciencias de la Producción y de la Salud Animal, Universidad Nacional Autónoma de México, Mexico.
authors.forename: J A
authors.initials: JA
authors.lastname: Buendía-Jiménez
authors.affiliation: Departamento de Ciencias Biológicas, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Mexico.
authors.forename: M A
authors.initials: MA
authors.lastname: Muñoz-Guzmán
authors.affiliation: Laboratorio de Inmunobiología de las Mucosas, Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Mexico.
authors.forename: M A
authors.initials: MA
authors.lastname: Vega-López
authors.affiliation: Departamento de Ciencias Biológicas, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Mexico.
authors.forename: C
authors.initials: C
authors.lastname: Cuenca-Verde
authors.affiliation: Departamento de Ciencias Biológicas, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Mexico.
authors.forename: J P
authors.initials: JP
authors.lastname: Martínez-Labat
authors.affiliation: Departamento de Ciencias Biológicas, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Mexico.
authors.forename: J A
authors.initials: JA
authors.lastname: Cuéllar-Ordaz
authors.affiliation: Departamento de Ciencias Biológicas, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Mexico. Electronic address: fealba@hotmail.com.
authors.forename: F
authors.initials: F
authors.lastname: Alba-Hurtado
mesh_terms: D000018:Abomasum / Q000276:immunology; D000818:Animals; D016207:Cytokines / Q000276:immunology*; D004804:Eosinophils / Q000276:immunology; D005260:Female; D006188:Haemonchiasis / Q000276:immunology / Q000662:veterinary*; D006189:Haemonchus / Q000276:immunology*; D007958:Leukocyte Count / Q000662:veterinary; D008297:Male; D008407:Mast Cells / Q000276:immunology; D010270:Parasite Egg Count / Q000662:veterinary; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology*; D013621:Taenia / Q000276:immunology*
chemical_list: D016207:Cytokines
keywords: Abomasal wall; Cytokines; Immune protection; Immunohistochemistry; Ovine haemonchosis