title: [2-methoxyestradiol induced apoptosis and expression of p53 gene in human acute T lymphoblastic leukemia cells].
abstract: OBJECTIVE
  To explore the effect of 2-methoxyestradiol (2-ME2) on apoptosis of human acute T lymphoblastic leukemia cells, and its underlying mechanism.
  
  
  METHODS
  The growth inhibition of CEM cells was detected by MTT assay; apoptotic cells were detected by DNA laddering analysis; the expressions of P53 mRNA and protein were detected by RT-PCR and Western blot respectively.
  
  
  RESULTS
  2-ME2 remarkably inhibited the CEM cell growth and the 50% growth inhibitory concentration (IC50) at 48 h was 2 µmol/L. The DNA ladder could be detected in CEM cells after treating with 2 µmol/L 2-ME2 for 24, 48 and 72 hours; after treating with 2 µmol/L 2-ME2 for 24, 48 and 72 hours, a time-dependent reduction of P53 mRNA and protein expressions was found in CEM cells.
  
  
  CONCLUSIONS
  The anti-leukemia effect of 2-ME2 is completed through the induction of cell apoptosis. Down-regulation of P53 gene expression may be an underlying mechanism.
authors.affiliation: Department of Hematology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, Fujian Province, China. E-mail: jakey3456@sina.com.
authors.forename: Xue-Ya
authors.initials: XY
authors.lastname: Zhang
authors.affiliation: Department of Hematology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, Fujian Province, China.
authors.forename: Shi-Xin
authors.initials: SX
authors.lastname: Wu
authors.affiliation: Department of Hematology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, Fujian Province, China.
authors.forename: Xi-Zhe
authors.initials: XZ
authors.lastname: Guo
authors.affiliation: Department of Hematology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, Fujian Province, China.
authors.forename: Jing-Xin
authors.initials: JX
authors.lastname: Pan
mesh_terms: D000077584:2-Methoxyestradiol; D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D004958:Estradiol / Q000031:analogs & derivatives; D016158:Genes, p53; D006801:Humans; D054218:Precursor T-Cell Lymphoblastic Leukemia-Lymphoma
chemical_list: D004958:Estradiol; D000077584:2-Methoxyestradiol