title: Characterization and antivirus activities of a novel bovine IFN-omega24.
abstract: A novel bovine interferon-ω (BoIFN-ω) gene, which encodes a protein of 195 amino acids with a 23-amino acid signal peptide, was amplified from bovine liver genomic DNA through PCR and named BoIFN-ω24 according to its position in the bovine genome. In this study, the recombinant protein was expressed in Escherichia coli and antiviral or antiproliferation activity was determined in vitro. Results showed that BoIFN-ω24 exhibits high antiviral activity, which can be abrogated using PAb against BoIFN-ω24, and inhibits cell proliferation. BoIFN-ω24 also presents high sensitivity to trypsin and stability at pH 2.0 or 65°C, which are typical characteristics of type I IFN. This study revealed that BoIFN-ω24 is a potential novel effective therapeutic agent and provided a basis for further research on the BoIFN-ω multigene family.
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, PR China.
authors.forename: Xiuxin
authors.initials: X
authors.lastname: Luo
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, PR China.
authors.forename: Yongli
authors.initials: Y
authors.lastname: Guo
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, Heilongjiang 150030, PR China; National Food Safety and Nutrition Collaborative Innovation Center, Wuxi, Jiangsu 214122, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Bao
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, PR China.
authors.forename: Dong
authors.initials: D
authors.lastname: An
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Ma
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, PR China. Electronic address: gaomingchun@163.com.
authors.forename: Mingchun
authors.initials: M
authors.lastname: Gao
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, PR China; National Food Safety and Nutrition Collaborative Innovation Center, Wuxi, Jiangsu 214122, PR China. Electronic address: jwwang@neau.edu.cn.
authors.forename: Junwei
authors.initials: J
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000998:Antiviral Agents / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism / Q000494:pharmacology; D001483:Base Sequence; D002417:Cattle; D002460:Cell Line; D049109:Cell Proliferation; D003412:Cricetulus; D004247:DNA / Q000145:classification / Q000276:immunology / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D007370:Interferon Type I / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology; D008099:Liver / Q000737:chemistry / Q000276:immunology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology; D055550:Protein Stability; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000494:pharmacology; D013552:Swine; D014357:Trypsin / Q000737:chemistry; D018116:Vesiculovirus / Q000187:drug effects* / Q000254:growth & development / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000998:Antiviral Agents; D007370:Interferon Type I; D020033:Protein Isoforms; D011994:Recombinant Proteins; C063743:interferon omega 1; D004247:DNA; D014357:Trypsin
keywords: Antiproliferation activity; Antiviral activity; Bovine; IFN-ω24; Physicochemical characteristics