title: Acute toxicological studies of the main organosulfur compound derived from Allium sp. intended to be used in active food packaging.
abstract: Some plant extracts have been proposed as potential alternative to the use of synthetic preservatives in the food industry. Among those, extracts from Allium species exhibit interesting antimicrobial and antioxidant properties for the food packaging industry. The present work aims to assess the usefulness and potential safety of the major organosulfur compound present in a commercial Allium sp. extract (PROALLIUM AP®), namely propyl thiosulfinate oxide (PTSO). For this purpose, its antimicrobial activity was studied in a wide range of microorganisms. Moreover, cytotoxicity and ultrastructural cellular damages caused by PTSO were studied in two human cell lines, Caco-2 and HepG2, being the colonic cells more sensitive to this compound. Finally, the protective role of PTSO against an induced oxidative situation was evaluated in the human intestinal Caco-2 cells. The results revealed damage at high concentration, although no significant adverse effects were recorded for the concentration to be used in food packaging. Moreover, the in vivo study also revealed the potential safety use at the established concentrations. In addition, the antimicrobial properties and the antioxidant role of PTSO were confirmed. Therefore, this compound could be considered as a good natural alternative to synthetic preservatives used in the food packaging industry.
authors.affiliation: Area of Toxicology, Faculty of Pharmacy, Universidad de Sevilla, Profesor García González n°2, 41012 Seville, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Llana-Ruiz-Cabello
authors.affiliation: Area of Toxicology, Faculty of Pharmacy, Universidad de Sevilla, Profesor García González n°2, 41012 Seville, Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: Gutiérrez-Praena
authors.affiliation: Area of Toxicology, Faculty of Pharmacy, Universidad de Sevilla, Profesor García González n°2, 41012 Seville, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Puerto
authors.affiliation: Area of Toxicology, Faculty of Pharmacy, Universidad de Sevilla, Profesor García González n°2, 41012 Seville, Spain. Electronic address: spichardo@us.es.
authors.forename: Silvia
authors.initials: S
authors.lastname: Pichardo
authors.affiliation: Area of Cellular Biology, Faculty of Biology, Universidad de Sevilla, Avda. Reina Mercedes s/n, 41012 Seville, Spain.
authors.forename: F Javier
authors.initials: FJ
authors.lastname: Moreno
authors.affiliation: DOMCA S.A., Camino de Jayena s/n, Alhendín, 18620 Granada, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Baños
authors.affiliation: DOMCA S.A., Camino de Jayena s/n, Alhendín, 18620 Granada, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Nuñez
authors.affiliation: DOMCA S.A., Camino de Jayena s/n, Alhendín, 18620 Granada, Spain.
authors.forename: Enrique
authors.initials: E
authors.lastname: Guillamón
authors.affiliation: Area of Toxicology, Faculty of Pharmacy, Universidad de Sevilla, Profesor García González n°2, 41012 Seville, Spain.
authors.forename: Ana María
authors.initials: AM
authors.lastname: Cameán
mesh_terms: D000490:Allium / Q000737:chemistry*; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D018938:Caco-2 Cells / Q000187:drug effects; D005260:Female; D018857:Food Packaging; D006801:Humans; D008826:Microbial Sensitivity Tests; D008854:Microscopy, Electron; D010936:Plant Extracts / Q000737:chemistry / Q000633:toxicity; D051381:Rats; D013441:Sulfinic Acids / Q000494:pharmacology* / Q000633:toxicity*; D023382:Toxicity Tests, Acute
chemical_list: D000890:Anti-Infective Agents; D000975:Antioxidants; D010936:Plant Extracts; D013441:Sulfinic Acids; C000608757:propyl thiosulfinate oxide
keywords: Allium sp; Antimicrobial; Antioxidant; Human cell line; Propyl thiosulfinate oxide; Rat