title: Selected oxidative stress markers in gynecological laparoscopy.
abstract: BACKGROUND
  The surgical stress response after laparoscopy is smaller when compared with open surgery, and it is expected that after minimally invasive surgery the possible development of oxidative stress will be less severe.
  
  
  OBJECTIVE
  To evaluate markers of pro-oxidant activity - levels of lipid peroxides and malondialdehyde - and activity of the antioxidant enzymes superoxide dismutase and glutathione peroxidase in the perioperative period in patients undergoing gynecological laparoscopy and to determine whether the duration of laparoscopy can affect these changes.
  
  
  METHODS
  The study included 64 patients, divided into two groups: group 1 with duration of laparoscopy up to 20 min, and group 2 with duration of the operation over 40 min. Blood samples were collected before anesthesia, 5 min after release of pneumoperitoneum, and 10 h after surgery.
  
  
  RESULTS
  A statistically significant increase in the levels of lipid peroxides and malondialdehyde in samples collected after surgery was found in comparison with values obtained before surgery. Also statistically significant differences existed between groups of patients with different duration of surgery. Superoxide dismutase and glutathione peroxidase activity values were significantly decreased. They were also significantly different between the two groups with different duration of surgery.
  
  
  CONCLUSIONS
  In our study, levels of the markers of pro-oxidant activity increased and levels of the markers of antioxidant enzymes decreased, suggesting development of oxidative stress. The duration of laparoscopic procedures affects the severity of the presented changes.
authors.affiliation: Department of Physiology, Poznan University of Medical Sciences, Poznan, Poland ; Department of Gynecology and Obstetrics, F. Raszeja Hospital, Poznan, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Koźlik
authors.affiliation: Department of Gynecology and Obstetrics, F. Raszeja Hospital, Poznan, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Przybyłowska
authors.affiliation: Department of Physiology, Poznan University of Medical Sciences, Poznan, Poland.
authors.forename: Kinga
authors.initials: K
authors.lastname: Mikrut
authors.affiliation: Department of Allergology and Environmental Hazards, Institute of Rural Health, Lublin, Poland.
authors.forename: Wioletta A
authors.initials: WA
authors.lastname: Żukiewicz-Sobczak
authors.affiliation: Department of Allergology and Environmental Hazards, Institute of Rural Health, Lublin, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Zwoliński
authors.affiliation: Department of Physiology, Poznan University of Medical Sciences, Poznan, Poland ; Department of Allergology and Environmental Hazards, Institute of Rural Health, Lublin, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Piątek
authors.affiliation: Department of Food Engineering and Machines, University of Life Sciences, Lublin, Poland.
authors.forename: Paweł
authors.initials: P
authors.lastname: Sobczak
keywords: duration of laparoscopy; gynecological laparoscopy; oxidative stress