title: Evaluation of carbohydrate metabolism inhibition by some species of medicinal mushrooms from India.
abstract: Diabetes mellitus is one of the most common endocrine diseases. One antidiabetic therapeutic approach is to reduce gastrointestinal glucose production and absorption through the inhibition of α-amylase and α-amyloglucosidase enzymes, thereby preventing an increase in the postprandial glucose concentration in diabetics. The main aim of this study was to evaluate the antidiabetic potential of hydroalcoholic extracts of 3 mushrooms, that is, Ganoderma philippii, Lenzites elegans, and Rigidoporus ulmarius, using an in vitro enzymatic starch digestion assay model. The α-amylase and α-glucosidase inhibitory potential of hydroalcoholic mushroom extracts was tested at concentrations of 1, 5, 10, 12.5, 25, 50, and 100 mg/mL. Acarbose was used as a control. The amount of glucose liberated (micrograms) was determined using the 3,5-dinitrosalicylic acid method. Phytochemical screening revealed the presence of carbohydrates in all examined species. In the case of G. philippii and L. elegans, a concentration-dependent increase in the percentage inhibition of enzyme activity was observed, with maximum inhibition at a concentration of 100 mg/mL (40.22% ± 0.83% and 26.57% ± 0.68%, respectively). R. ulmarius showed maximum inhibitory activity at a concentration of 100 mg/mL (65.54% ± 0.91%), and this was comparable to acarbose.
authors.affiliation: Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Punjab, India.
authors.forename: Amandip
authors.initials: A
authors.lastname: Kaur
authors.affiliation: Department of Botany, Punjabi University, Punjab, India.
authors.forename: G S
authors.initials: GS
authors.lastname: Dhingra
authors.affiliation: Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Punjab, India.
authors.forename: Richa
authors.initials: R
authors.lastname: Shri
mesh_terms: D050260:Carbohydrate Metabolism; D045424:Complex Mixtures / Q000302:isolation & purification / Q000494:pharmacology*; D055453:Coriolaceae / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D032902:Ganoderma / Q000737:chemistry*; D006801:Humans; D007004:Hypoglycemic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D007194:India; D000516:alpha-Amylases / Q000037:antagonists & inhibitors; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D045424:Complex Mixtures; D004791:Enzyme Inhibitors; D007004:Hypoglycemic Agents; D000516:alpha-Amylases; D000520:alpha-Glucosidases