title: Peripheral gene expression profile of mechanical hyperalgesia induced by repeated cold stress in SHRSP5/Dmcr rats.
abstract: Repeated cold stress (RCS) is known to transiently induce functional disorders associated with hypotension and hyperalgesia. In this study, we investigated the effects of RCS (24 and 4 °C alternately at 30-min intervals during the day and 4 °C at night for 2 days, followed by 4 °C on the next 2 consecutive nights) on the thresholds for cutaneous mechanical pain responses and on peripheral expression of "pain-related genes" in SHRSP5/Dmcr rats, which are derived from stroke-prone spontaneously hypertensive rats. To define genes peripherally regulated by RCS, we detected changes in the expression of pain-related genes in dorsal root ganglion cells by PCR-based cDNA subtraction analysis or DNA microarray analysis, and confirmed the changes by RT-PCR. We found significantly changed expression in eight pain-related genes (upregulated: Fyn, St8sia1, and Tac 1; downregulated: Ctsb, Fstl1, Itpr1, Npy, S100a10). At least some of these genes may play key roles in hyperalgesia induced by RCS.
authors.affiliation: College of Pharmacy, Kinjo Gakuin University, 2-1723 Ohmori, Moriyama-ku, Nagoya, 463-8521, Japan, kozaki@kinjo-u.ac.jp.
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Kozaki
authors.forename: Rena
authors.initials: R
authors.lastname: Umetsu
authors.forename: Yukako
authors.initials: Y
authors.lastname: Mizukami
authors.forename: Aya
authors.initials: A
authors.lastname: Yamamura
authors.forename: Kazuya
authors.initials: K
authors.lastname: Kitamori
authors.forename: Satoru
authors.initials: S
authors.lastname: Tsuchikura
authors.forename: Katsumi
authors.initials: K
authors.lastname: Ikeda
authors.forename: Yukio
authors.initials: Y
authors.lastname: Yamori
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000235:genetics; D003080:Cold Temperature; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation; D006930:Hyperalgesia / Q000209:etiology / Q000235:genetics* / Q000503:physiopathology; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D017288:Pain Threshold; D011918:Rats, Inbred SHR; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013312:Stress, Physiological; D013997:Time Factors