title: Complexes of green tea polyphenol, epigalocatechin-3-gallate, and 2S albumins of peanut.
abstract: 2S albumins of peanuts are seed storage proteins, highly homologous in structure and described as major elicitors of anaphylactic reactions to peanut (allergens Ara h 2 and Ara h 6). Epigallocatechin-3-gallate (EGCG) is the most biologically potent polyphenol of green tea. Non-covalent interactions of EGCG with proteins contribute to its diverse biological activities. Here we used the methods of circular dichroism, fluorescence quenching titration, isothermal titration calorimetry and computational chemistry to elucidate interactions of EGCG and 2S albumins. Similarity in structure and overall fold of 2S albumins yielded similar putative binding sites and similar binding modes with EGCG. Binding affinity determined for Ara h 2 was in the range described for complexes of EGCG and other dietary proteins. Binding of EGCG to 2S albumins affects protein conformation, by causing an α-helix to β-structures transition in both proteins. 2S albumins of peanuts may be good carriers of physiologically active green tea catechin.
authors.affiliation: University of Belgrade, Faculty of Chemistry, Center of Excellence for Molecular Food Sciences, Serbia.
authors.forename: Jelena
authors.initials: J
authors.lastname: Vesic
authors.affiliation: University of Belgrade, Faculty of Chemistry, Center of Excellence for Molecular Food Sciences, Serbia.
authors.forename: Ivan
authors.initials: I
authors.lastname: Stambolic
authors.affiliation: University of Belgrade, Faculty of Chemistry, Center of Excellence for Molecular Food Sciences, Serbia; Department of Medicine Solna, Clinical Immunology and Allergy Unit, Karolinska Institutet and University Hospital, Stockholm, Sweden.
authors.forename: Danijela
authors.initials: D
authors.lastname: Apostolovic
authors.affiliation: University of Belgrade, Faculty of Chemistry, Center of Excellence for Molecular Food Sciences, Serbia.
authors.forename: Milos
authors.initials: M
authors.lastname: Milcic
authors.affiliation: University of Belgrade, Faculty of Chemistry, Center of Excellence for Molecular Food Sciences, Serbia.
authors.forename: Dragana
authors.initials: D
authors.lastname: Stanic-Vucinic
authors.affiliation: University of Belgrade, Faculty of Chemistry, Center of Excellence for Molecular Food Sciences, Serbia. Electronic address: tcirkov@chem.bg.ac.rs.
authors.forename: Tanja
authors.initials: T
authors.lastname: Cirkovic Velickovic
mesh_terms: D055516:2S Albumins, Plant / Q000737:chemistry*; D010367:Arachis / Q000737:chemistry*; D001665:Binding Sites; D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry; D002942:Circular Dichroism; D017433:Protein Structure, Secondary; D013662:Tea / Q000737:chemistry*
chemical_list: D055516:2S Albumins, Plant; D013662:Tea; D002392:Catechin; C045651:epigallocatechin gallate
keywords: 2S albumins; Binding affinity; Epigallocatechin-3-gallate; Fluorophore quenching; Molecular docking; Peanut; Polyphenol