title: Effects of the inhaled treatment of liriope radix on an asthmatic mouse model.
abstract: As a treatment for allergic asthma, inhaled treatments such as bronchodilators that contain β2-agonists have an immediate effect, which attenuates airway obstructions and decreases airway hypersensitivity. However, bronchodilators only perform on a one off basis, but not consistently. Asthma is defined as a chronic inflammatory disease of the airways accompanying the overproduction of mucus, airway wall remodeling, bronchial hyperreactivity and airway obstruction. Liriope platyphylla radix extract (LPP), a traditional Korean medicine, has been thoroughly studied and found to be an effective anti-inflammatory medicine. Here, we demonstrate that an inhaled treatment of LPP can attenuate airway hyperresponsiveness (AHR) in an ovalbumin-induced asthmatic mouse model, compared to the saline-treated group (p < 0.01). Moreover, LPP decreases inflammatory cytokine levels, such as eotaxin (p < 0.05), IL-5 (p < 0.05), IL-13 (p < 0.001), RANTES (p < 0.01), and TNF-α (p < 0.05) in the bronchoalveolar lavage (BAL) fluid of asthmatic mice. A histopathological study was carried out to determine the effects of LPP inhalation on mice lung tissue. We performed UPLC/ESI-QTOF-MS, LC/MS, and GC/MS analyses to analyze the chemical constituents of LPP, finding that these are ophiopogonin D, spicatoside A, spicatoside B, benzyl alcohol, and 5-hydroxymethylfurfural. This study demonstrates the effect of an inhaled LPP treatment both on airway AHR and on the inflammatory response in an asthmatic mouse model. Hence, LPP holds significant promise as a nasal inhalant for the treatment of asthmatic airway disease.
authors.affiliation: College of Korean Medicine, Institute of Korean Medicine, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Ki-Suk
authors.initials: KS
authors.lastname: Kim
authors.forename: Dong-Hyuk
authors.initials: DH
authors.lastname: Cho
authors.forename: Hea Jung
authors.initials: HJ
authors.lastname: Yang
authors.forename: Eun-Kyeong
authors.initials: EK
authors.lastname: Choi
authors.forename: Min Hee
authors.initials: MH
authors.lastname: Shin
authors.forename: Kang-Hoon
authors.initials: KH
authors.lastname: Kim
authors.forename: Kwang Seok
authors.initials: KS
authors.lastname: Ahn
authors.forename: In Jin
authors.initials: IJ
authors.lastname: Ha
authors.forename: Yun-Cheol
authors.initials: YC
authors.lastname: Na
authors.forename: Jae Young
authors.initials: JY
authors.lastname: Um
authors.forename: Won Seok
authors.initials: WS
authors.lastname: Chung
authors.forename: Hee-Jae
authors.initials: HJ
authors.lastname: Jung
authors.forename: Sung-Ki
authors.initials: SK
authors.lastname: Jung
authors.forename: Hyeung-Jin
authors.initials: HJ
authors.lastname: Jang
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D001249:Asthma / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D016207:Cytokines / Q000378:metabolism; D003864:Depression, Chemical; D004195:Disease Models, Animal; D005260:Female; D018836:Inflammation Mediators / Q000378:metabolism; D031424:Liriope Plant; D055670:Medicine, Korean Traditional; D008807:Mice, Inbred BALB C; D010047:Ovalbumin; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage* / Q000494:pharmacology; D012130:Respiratory Hypersensitivity / Q000139:chemically induced / Q000188:drug therapy*
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D010936:Plant Extracts; D010047:Ovalbumin
keywords: Airway Hyperresponsiveness; Airway Inflammation; Asthma; Bronchodilation; Liriope platyphylla Radix Extract