title: Evaluation of Candida Colonization and Specific Humoral Responses against Candida albicans in Patients with Atopic Dermatitis.
abstract: The aim of this study was to assess the candidal colonization and specific humoral responses against Candida albicans in patients with atopic dermatitis. One hundred patients with atopic dermatitis and 50 healthy individuals were enrolled in the study. Skin and oral specimens from all participants were cultured on CHROMagar Candida medium. Isolated yeasts were identified by using the sequence of the D1/D2 domain of the 26S rRNA gene. ELISA was used for detection of IgM, IgA, and IgG antibodies against C. albicans in sera of participants. Candida species were isolated from the skin and oral cavity of 31% of the patients and 12% of the controls. There was no significant difference between Candida colonization in patients and controls (P > 0.05). Candida albicans was isolated from the skin and oral cavity of 23% of the patients and 6% of the controls (P < 0.05). There were no significant differences between serum levels of IgM and IgA in patients and controls (P > 0.05). Serum level of IgG was significantly lower in patients than in controls (P < 0.05). Type of Candida colonization can change in patients with atopic dermatitis. In addition, these patients have abnormalities in the production of antibodies against Candida albicans that may have a role in the pathogenesis of atopic dermatitis.
authors.affiliation: Department of Pediatrics, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Ghaffari
authors.initials: G
authors.lastname: Javad
authors.affiliation: Department of Medical Mycology and Parasitology, School of Medicine, Jiroft University of Medical Sciences, Jiroft, Iran ; Department of Medical Mycology and Parasitology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Taheri Sarvtin
authors.affiliation: Department of Medical Mycology and Parasitology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran ; Invasive Fungi Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Mohammad Taghi
authors.initials: MT
authors.lastname: Hedayati
authors.affiliation: Invasive Fungi Research Center, Mazandaran University of Medical Sciences, Sari, Iran ; Department of Dermatology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Zohreh
authors.initials: Z
authors.lastname: Hajheydari
authors.affiliation: Department of Statistics, School of Health, Mazandaran University of Medical Science, Sari, Iran.
authors.forename: Jamshid
authors.initials: J
authors.lastname: Yazdani
authors.affiliation: Department of Medical Mycology and Parasitology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran ; Invasive Fungi Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Tahereh
authors.initials: T
authors.lastname: Shokohi
mesh_terms: D000328:Adult; D000908:Antibodies, Fungal / Q000097:blood / Q000276:immunology; D002176:Candida albicans / Q000276:immunology* / Q000378:metabolism; D002177:Candidiasis / Q000097:blood / Q000276:immunology / Q000473:pathology; D003876:Dermatitis, Atopic / Q000097:blood / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D005260:Female; D006801:Humans; D056724:Immunity, Humoral; D008297:Male; D008875:Middle Aged; D009055:Mouth / Q000276:immunology / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D012867:Skin / Q000276:immunology / Q000378:metabolism / Q000382:microbiology / Q000473:pathology
chemical_list: D000908:Antibodies, Fungal