title: Hemolysis-free plasma miR-214 as novel biomarker of gastric cancer and is correlated with distant metastasis.
abstract: Circulating miRNAs gains popularity for its potential ability to serve as biomarkers of cancer. The aim of present study was to evaluate the usefulness of plasma miR-214 as novel biomarkers for gastric cancer (GC) detection. Attempts were made to address several pitfalls in sample processing and study design in previous studies. We conducted a two-step analysis: (1) in pilot study comprising of 30 patients and 30 controls, levels of miR-214 were significantly higher in primary GC tissues than normal tissues (P = 0.0215). Plasma miR-214 was significantly higher in patients with GC than in controls (P < 0.0001). (2) In test of larger cohort, there was significantly decreasing tendency of plasma miR-214 from patients before, 14 days and 1 month after surgical resection (P < 0.0001). There were significantly higher levels of miR-214 in 80 GC patients than in 70 controls (P < 0.0001). Receiver operating characteristics (ROC) curves yielded area under the curve (AUC) value of 0.845. Moreover, high plasma miR-214 had significant correlation with distant metastasis (P = 0.038). Thus, our data suggest that plasma miR-214 was novel hemolysis-free markers of gastric cancer.
authors.affiliation: Department of General Surgery, Chinese People's Liberation Army General Hospital 28 Fuxing Road, Beijing 100853, China.
authors.forename: Ke-Cheng
authors.initials: KC
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, Chinese People's Liberation Army General Hospital 28 Fuxing Road, Beijing 100853, China.
authors.forename: Hong-Qing
authors.initials: HQ
authors.lastname: Xi
authors.affiliation: Department of General Surgery, Chinese People's Liberation Army General Hospital 28 Fuxing Road, Beijing 100853, China.
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Cui
authors.affiliation: Department of General Surgery, Chinese People's Liberation Army General Hospital 28 Fuxing Road, Beijing 100853, China.
authors.forename: Wei-Song
authors.initials: WS
authors.lastname: Shen
authors.affiliation: Department of General Surgery, Chinese People's Liberation Army General Hospital 28 Fuxing Road, Beijing 100853, China.
authors.forename: Ji-Yang
authors.initials: JY
authors.lastname: Li
authors.affiliation: Department of General Surgery, Chinese People's Liberation Army General Hospital 28 Fuxing Road, Beijing 100853, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wei
authors.affiliation: Department of General Surgery, Chinese People's Liberation Army General Hospital 28 Fuxing Road, Beijing 100853, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
keywords: Plasma miR-214; biomarkers; distant metastasis; gastric cancer