title: Phospholipid polymer electrodeposited on titanium inhibits platelet adhesion.
abstract: To develop metallic materials with thromboresistance, a block-type copolymer (PMbA) was immobilized onto a titanium surface with electrodeposition. The polymer was composed of a poly(2-(methacryloyloxy)ethyl phosphorylcholine (MPC)) segment and a poly(2-aminoethylmethacrylate) segment, which was designed to electronically adsorb to the surface oxide layer on the titanium surface. We confirmed that the polymer was synthesized as expected by nuclear magnetic resonance and gel permeation chromatography. In a 0.26 mmol L(-1) PMbA solution adjusted to pH 11, -3.0 V (vs. an Ag/AgCl electrode) was applied to a titanium substrate for 300 s. The immobilization of PMbA on the titanium was confirmed with X-ray photoelectron spectroscopy and an atomic force microscope. The water contact angle and amount of adsorbed protein on the PMbA-modified surface were decreased. Thrombus formation was not observed all over the surface even when the surface was contacted with human blood without an anticoagulant. Therefore, PMbA covered the surface with even density and poly(MPC) segments in PMbA inhibited platelet adsorption. Electrodeposition with PMbA improves the blood compatibility of a titanium substrate in a simple process.
authors.affiliation: Department of Materials Engineering, School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo, 113-8656, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Fukuhara
authors.affiliation: Department of Materials Engineering, School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo, 113-8656, Japan.
authors.forename: Megumi
authors.initials: M
authors.lastname: Kyuzo
authors.affiliation: Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Chiyoda-ku, Tokyo, 101-0062, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Tsutsumi
authors.affiliation: Department of Materials Engineering, School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo, 113-8656, Japan.|Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Chiyoda-ku, Tokyo, 101-0062, Japan.
authors.forename: Akiko
authors.identifier: http://orcid.org/0000-0002-0813-7728
authors.initials: A
authors.lastname: Nagai
authors.affiliation: Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Chiyoda-ku, Tokyo, 101-0062, Japan.
authors.forename: Peng
authors.initials: P
authors.lastname: Chen
authors.affiliation: Department of Materials Engineering, School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo, 113-8656, Japan.|Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Chiyoda-ku, Tokyo, 101-0062, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Hanawa
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D001792:Blood Platelets / Q000378:metabolism*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D010767:Phosphorylcholine / Q000737:chemistry*; D010973:Platelet Adhesiveness; D014025:Titanium / Q000737:chemistry
chemical_list: D000180:Acrylic Resins; D020099:Coated Materials, Biocompatible; D010767:Phosphorylcholine; D014025:Titanium
keywords: MPC polymer; electrodeposition; protein adsorption resistance; thrombus formation resistance; titanium