title: A liquid chromatography with tandem mass spectrometry method for simultaneous determination of UTL-5g and its metabolites in human plasma.
abstract: UTL-5g is a novel small-molecule TNF-α inhibitor under investigation as both a chemoprotective and radioprotective agent. Animal studies showed that pretreatment of UTL-5g protected kidney, liver, and platelets from cisplatin-induced toxicity. In addition, UTL-5g reduced liver and lung injuries induced by radiation in vivo. Although a number of preclinical studies have been conducted, a validated bioanalytical method for UTL-5g in human plasma has not been published. In this work, a sensitive and reproducible reverse-phase liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) assay was developed and validated for the determination of UTL-5g and its metabolites, 5-methylisoxazole-3-carboxylic acid (ISOX) and 2,4-dichloroaniline (DCA), in human plasma. The method involves a simple methanol precipitation step followed by injection of the supernatant onto a Waters 2695 HPLC system coupled with a Waters Quattro Micro™ triple quadrupole mass spectrometer. Chromatographic separation was accomplished using a Waters Nova-Pak C18 column maintained at 30°C, running at gradient mode with mobile phase consisting of 0.1% formic acid in water and 0.1% formic acid in methanol at a flow rate of 0.2mL/min. The analytes were monitored under positive electrospray ionization (ESI). Quantitation of these compounds in plasma was linear from 0.05 to 10μM. The lower limit of quantitation (LLOQ) was 0.05, 0.1, and 0.2μM for UTL-5g, ISOX and DCA, respectively. The accuracy and intra-and inter-day precisions were within the generally accepted criteria for bioanalytical method (<15%). This method provides a practical tool to measure and characterize the plasma concentration-time profiles for UTL-5g and its metabolites, ISOX and DCA.
authors.affiliation: 21st Century Therapeutics, Inc., 440 Burroughs, Suite 447 Detroit, MI 48202, United States. Electronic address: jiajiushaw@gmail.com.
authors.forename: Jiajiu
authors.initials: J
authors.lastname: Shaw
authors.affiliation: Karmanos Cancer Institute, Wayne State University School of Medicine, Detroit, MI 48201, United States.
authors.forename: Richard
authors.initials: R
authors.lastname: Wiegand
authors.affiliation: Karmanos Cancer Institute, Wayne State University School of Medicine, Detroit, MI 48201, United States.
authors.forename: Jianmei
authors.initials: J
authors.lastname: Wu
authors.affiliation: Karmanos Cancer Institute, Wayne State University School of Medicine, Detroit, MI 48201, United States.
authors.forename: Xun
authors.initials: X
authors.lastname: Bao
authors.affiliation: Henry Ford Health System, Internal Medicine, Detroit, MI 48202, United States.
authors.forename: Frederick
authors.initials: F
authors.lastname: Valeriote
authors.affiliation: Karmanos Cancer Institute, Wayne State University School of Medicine, Detroit, MI 48201, United States. Electronic address: lijin@karmanos.org.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
mesh_terms: D000814:Aniline Compounds / Q000097:blood / Q000378:metabolism; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D006801:Humans; D007555:Isoxazoles / Q000097:blood* / Q000378:metabolism*; D057230:Limit of Detection; D053719:Tandem Mass Spectrometry / Q000379:methods; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors*
chemical_list: D000814:Aniline Compounds; D007555:Isoxazoles; C439524:TNF protein, human; D014409:Tumor Necrosis Factor-alpha; C559524:UTL-5g compound; C054619:2,4-dichloroaniline
keywords: Bioanalytical method validation; LC–MS/MS; UTL-5g