title: Development of a novel cell sorting method that samples population diversity in flow cytometry.
abstract: Flow cytometry based electrostatic cell sorting is an important tool in the separation of cell populations. Existing instruments can sort single cells into multi-well collection plates, and keep track of cell of origin and sorted well location. However currently single sorted cell results reflect the population distribution and fail to capture the population diversity. Software was designed that implements a novel sorting approach, "Slice and Dice Sorting," that links a graphical representation of a multi-well plate to logic that ensures that single cells are sampled and sorted from all areas defined by the sort region/s. Therefore the diversity of the total population is captured, and the more frequently occurring or rarer cell types are all sampled. The sorting approach was tested computationally, and using functional cell based assays. Computationally we demonstrate that conventional single cell sorting can sample as little as 50% of the population diversity dependant on the population distribution, and that Slice and Dice sorting samples much more of the variety present within a cell population. We then show by sorting single cells into wells using the Slice and Dice sorting method that there are cells sorted using this method that would be either rarely sorted, or not sorted at all using conventional single cell sorting approaches. The present study demonstrates a novel single cell sorting method that samples much more of the population diversity than current methods. It has implications in clonal selection, stem cell sorting, single cell sequencing and any areas where population heterogeneity is of importance.
authors.affiliation: Queensland Brain Institute, University of Queensland, Brisbane, QLD, 4072, Australia.|The Australian Institute for Bioengineering and Nanotechnology, University of Queensland, Brisbane, QLD 4072, Australia.
authors.forename: Geoffrey W
authors.initials: GW
authors.lastname: Osborne
authors.affiliation: The Australian Institute for Bioengineering and Nanotechnology, University of Queensland, Brisbane, QLD 4072, Australia.
authors.forename: Stacey B
authors.initials: SB
authors.lastname: Andersen
authors.affiliation: Frank Battye Flow Cytometry Consulting, Viewbank, VIC 3084, Australia.
authors.forename: Francis L
authors.initials: FL
authors.lastname: Battye
mesh_terms: D000465:Algorithms; D002469:Cell Separation / Q000379:methods; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D059010:Single-Cell Analysis / Q000379:methods*; D012984:Software; D013223:Statistics as Topic / Q000379:methods
keywords: Key terms: single cell sorting; index sorting