title: A NAD-dependent glutamate dehydrogenase coordinates metabolism with cell division in Caulobacter crescentus.
abstract: Coupling cell cycle with nutrient availability is a crucial process for all living cells. But how bacteria control cell division according to metabolic supplies remains poorly understood. Here, we describe a molecular mechanism that coordinates central metabolism with cell division in the α-proteobacterium Caulobacter crescentus. This mechanism involves the NAD-dependent glutamate dehydrogenase GdhZ and the oxidoreductase-like KidO. While enzymatically active GdhZ directly interferes with FtsZ polymerization by stimulating its GTPase activity, KidO bound to NADH destabilizes lateral interactions between FtsZ protofilaments. Both GdhZ and KidO share the same regulatory network to concomitantly stimulate the rapid disassembly of the Z-ring, necessary for the subsequent release of progeny cells. Thus, this mechanism illustrates how proteins initially dedicated to metabolism coordinate cell cycle progression with nutrient availability.
authors.affiliation: Bacterial Cell Cycle & Development (BCcD), URBM, University of Namur, Namur, Belgium.
authors.forename: François
authors.initials: F
authors.lastname: Beaufay
authors.affiliation: Bacterial Cell Cycle & Development (BCcD), URBM, University of Namur, Namur, Belgium.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Coppine
authors.affiliation: Bacterial Cell Cycle & Development (BCcD), URBM, University of Namur, Namur, Belgium.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Mayard
authors.affiliation: de Duve Institute, Université catholique de Louvain, Brussels, Belgium.
authors.forename: Géraldine
authors.initials: G
authors.lastname: Laloux
authors.affiliation: Bacterial Cell Cycle & Development (BCcD), URBM, University of Namur, Namur, Belgium.
authors.forename: Xavier
authors.initials: X
authors.lastname: De Bolle
authors.affiliation: Bacterial Cell Cycle & Development (BCcD), URBM, University of Namur, Namur, Belgium regis.hallez@unamur.be.
authors.forename: Régis
authors.initials: R
authors.lastname: Hallez
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D016935:Caulobacter crescentus / Q000166:cytology* / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division / Q000235:genetics*; D003598:Cytoskeletal Proteins / Q000378:metabolism; D017353:Gene Deletion; D005969:Glutamate Dehydrogenase / Q000378:metabolism / Q000502:physiology*; D009243:NAD / Q000378:metabolism; D030781:Organisms, Genetically Modified; D011485:Protein Binding
chemical_list: D001426:Bacterial Proteins; D003598:Cytoskeletal Proteins; C071525:FtsZ protein, Bacteria; D009243:NAD; D005969:Glutamate Dehydrogenase
keywords: FtsZ; GdhZ; cell division; cytokinesis; glutamate dehydrogenase