title: Sulfated Polysaccharide Isolated from the Sea Cucumber Stichopus japonicus Against PC12 Hypoxia/Reoxygenation Injury by Inhibition of the MAPK Signaling Pathway.
abstract: In this report, the sulfated polysaccharide (SJP) isolated from the sea cucumber Stichopus japonicus can protect PC12 from Na2S2O4-induced hypoxia/reoxygenation (H/R) injury. SJP effectively improves cell viability and reduces extracellular LDH release in PC12 cells after H/R. Moreover, SJP significantly increases SOD activity but decreases MDA levels. Our experiments showed that SJP could significantly reduce cell apoptosis caused by H/R. Our current results demonstrate that SJP suppressed the activation of MAPKs, resulting in a significant decrease in Bax/Bcl-2 ratio, cleaved caspase-3/caspase-3, p53 phosphorylation, and cytochrome c release in a concentration-dependent manner. MAPK is closely related to H/R injury. SJP inhibited JNK1/2 and p38 MAPK activation but did not affect the increased ERK1/2 expression. These results suggested that JNK1/2 and p38 MAPK pathways could be involved in SJP-mediated attenuation of PC12 H/R injury. SJP prevented PC12 H/R injury in a dose-dependent manner, indicating that SJP may be developed as a candidate drug to prevent or treat cerebral ischemia-reperfusion injury.
authors.affiliation: Marine College, Shandong University, Weihai, Shandong, China.|School of Pharmaceutical Sciences, Shandong University, Jinan, Shandong, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Cui
authors.affiliation: Marine College, Shandong University, Weihai, Shandong, China.
authors.forename: Ningshan
authors.initials: N
authors.lastname: Cui
authors.affiliation: School of Pharmaceutical Sciences, Shandong University, Jinan, Shandong, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.affiliation: Marine College, Shandong University, Weihai, Shandong, China.
authors.forename: Shuliang
authors.initials: S
authors.lastname: Song
authors.affiliation: Marine College, Shandong University, Weihai, Shandong, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Liang
authors.affiliation: Marine College, Shandong University, Weihai, Shandong, China. jiaiguo@sdu.edu.cn.|School of Pharmaceutical Sciences, Shandong University, Jinan, Shandong, China. jiaiguo@sdu.edu.cn.
authors.forename: Aiguo
authors.initials: A
authors.lastname: Ji
mesh_terms: D000818:Animals; D015687:Cell Hypoxia / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D020935:MAP Kinase Signaling System / Q000187:drug effects* / Q000502:physiology; D016716:PC12 Cells; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology*; D047428:Protein Kinase Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology*; D051381:Rats; D012616:Sea Cucumbers; D047528:Stichopus
chemical_list: D011134:Polysaccharides; D047428:Protein Kinase Inhibitors
keywords: Hypoxia/reoxygenation(H/R); MAPK; Na2S2O4; PC12