title: [Individual catalytic activity of two functional domains of bovicin HJ50 synthase BovM].
abstract: OBJECTIVE
  To reconstitute the in vitro catalytic activity of the individual dehydratase or cyclase domain of bifunctional bovicin HJ50 synthase BovM, and lay a foundation for the further investigation of catalytic mechanism of class II lantibiotic synthase LanM.
  
  
  METHODS
  The truncated proteins of BovM containing the N-terminal dehydratase domain or C-terminal cyclase domain were expressed in E. coli and purified. Substrate BovA, the precursor of bovicin HJ50, was incubated with these truncated BovM proteins in in vitro reaction system. The antimicrobial activity assay and MALDI-TOF MS analysis were used to monitor the dehydratase or cyclase activity of these truncated proteins. Meanwhile, the synergistic activities of both truncated proteins were tested in vivo and in vitro.
  
  
  RESULTS
  The N- and C-terminal domains of BovM possessed dehydration and cyclization activity respectively. However, no synergistic activity was detected between these two functional domains.
  
  
  CONCLUSIONS
  The individual functional domains of BovM could execute their corresponding functions independently, but the intactness of BovM was important for its full modification activity.
authors.forename: Hongchu
authors.initials: H
authors.lastname: Ma
authors.forename: Yong
authors.initials: Y
authors.lastname: Gao
authors.forename: Fangyuan
authors.initials: F
authors.lastname: Zhao
authors.forename: Jin
authors.initials: J
authors.lastname: Zhong
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D001430:Bacteriocins / Q000737:chemistry / Q000378:metabolism*; D055162:Biocatalysis; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D016985:Streptococcus bovis / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D001430:Bacteriocins; C000591653:bovicin HJ50, Streptococcus bovis