title: Analysis of microRNA Microarrays in Cardiogenesis.
abstract: microRNAs are a subclass of noncoding RNAs which have been demonstrated to play pivotal roles in multiple cellular mechanisms. microRNAs are small RNA molecules of 22-24 nt in length capable of modulating protein translation and/or RNA stability by base-priming with complementary sequences of the mRNAs, normally at the 3'untranslated region. To date, over 2,000 microRNAs have been already identified in humans, and orthologous microRNAs have been also identified in distinct animals and plants ranging a wide vast of species. High-throughput analyses by microarrays have become a gold standard to analyze the changes on microRNA expression in normal and pathological cellular or tissue conditions. In this chapter, we provide insights into the usage of this uprising technology in the context of cardiac development and disease.
authors.affiliation: Cardiovascular Development Group, Department of Experimental Biology, University of Jaén, Edificio B-3, Campus Las Lagunillas, Jaen, 23071, Spain. dfranco@ujaen.es.
authors.forename: Diego
authors.initials: D
authors.lastname: Franco
authors.affiliation: Cardiovascular Development Group, Department of Experimental Biology, University of Jaén, Edificio B-3, Campus Las Lagunillas, Jaen, 23071, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: Bonet
authors.affiliation: Cardiovascular Development Group, Department of Experimental Biology, University of Jaén, Edificio B-3, Campus Las Lagunillas, Jaen, 23071, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Hernandez-Torres
authors.affiliation: Cardiovascular Development Group, Department of Experimental Biology, University of Jaén, Edificio B-3, Campus Las Lagunillas, Jaen, 23071, Spain.
authors.forename: Estefania
authors.initials: E
authors.lastname: Lozano-Velasco
authors.affiliation: System Biology Group, Department of Experimental Biology, University of Jaén, Jaén, Spain.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Esteban
authors.affiliation: Cardiovascular Development Group, Department of Experimental Biology, University of Jaén, Edificio B-3, Campus Las Lagunillas, Jaen, 23071, Spain.
authors.forename: Amelia E
authors.initials: AE
authors.lastname: Aranega
mesh_terms: D000818:Animals; D019295:Computational Biology / Q000379:methods*; D020869:Gene Expression Profiling / Q000379:methods*; D018507:Gene Expression Regulation, Developmental; D006321:Heart / Q000196:embryology*; D006801:Humans; D015201:Meta-Analysis as Topic; D035683:MicroRNAs / Q000235:genetics*; D009206:Myocardium / Q000378:metabolism*; D038081:Organogenesis / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D059467:Transcriptome
chemical_list: D035683:MicroRNAs
keywords: Cardiac development; Meta-analyses; Microarrays; microRNA