title: Lipopolysaccharide pretreatment inhibits LPS-induced human umbilical cord mesenchymal stem cell apoptosis via upregulating the expression of cellular FLICE-inhibitory protein.
abstract: Mesenchymal stem cell (MSC)-based regenerative therapy is currently regarded as a novel approach with which to repair damaged tissues. However, the efficiency of MSC transplantation is limited due to the low survival rate of engrafted MSCs. Lipopolysaccharide (LPS) production is increased in numerous diseases and serves an essential function in the regulation of apoptosis in a variety of cell types. Previous studies have indicated that low-dose LPS pretreatment contributes to cytoprotection. In the current study, LPS was demonstrated to induce apoptosis in human umbilical cord mesenchymal stem cells (hUCMSCs) via the activation of caspase, in a dose-dependent manner. Low-dose LPS pretreatment may protect hUCMSCs against apoptosis induced by high-dose LPS, by upregulating the expression of cellular FADD-like IL-1β-converting enzyme-inhibitory protein (c-FLIP). The results of the present study indicate that pretreatment with an appropriate concentration of LPS may alleviate high-dose LPS-induced apoptosis.
authors.affiliation: Department of Burns and Plastic Surgery, The First Affiliated Hospital of PLA General Hospital, Beijing 100048, P.R. China.
authors.forename: Yu Sen
authors.initials: YS
authors.lastname: Hou
authors.affiliation: Department of Burns and Plastic Surgery, The First Affiliated Hospital of PLA General Hospital, Beijing 100048, P.R. China.
authors.forename: Ling Ying
authors.initials: LY
authors.lastname: Liu
authors.affiliation: Department of Burns and Plastic Surgery, The First Affiliated Hospital of PLA General Hospital, Beijing 100048, P.R. China.
authors.forename: Jia Ke
authors.initials: JK
authors.lastname: Chai
authors.affiliation: Department of Burns and Plastic Surgery, The First Affiliated Hospital of PLA General Hospital, Beijing 100048, P.R. China.
authors.forename: Yong Hui
authors.initials: YH
authors.lastname: Yu
authors.affiliation: Department of Burns and Plastic Surgery, The First Affiliated Hospital of PLA General Hospital, Beijing 100048, P.R. China.
authors.forename: Hong Jie
authors.initials: HJ
authors.lastname: Duan
authors.affiliation: Department of Burns and Plastic Surgery, The First Affiliated Hospital of PLA General Hospital, Beijing 100048, P.R. China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Hu
authors.affiliation: Department of Burns and Plastic Surgery, The First Affiliated Hospital of PLA General Hospital, Beijing 100048, P.R. China.
authors.forename: Hui Nan
authors.initials: HN
authors.lastname: Yin
authors.affiliation: Department of Burns and Plastic Surgery, The First Affiliated Hospital of PLA General Hospital, Beijing 100048, P.R. China.
authors.forename: Yi He
authors.initials: YH
authors.lastname: Wang
authors.affiliation: Department of Burns and Plastic Surgery, The First Affiliated Hospital of PLA General Hospital, Beijing 100048, P.R. China.
authors.forename: Shu Bo
authors.initials: SB
authors.lastname: Zhuang
authors.affiliation: Department of Burns and Plastic Surgery, The First Affiliated Hospital of PLA General Hospital, Beijing 100048, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Fan
authors.affiliation: Department of Burns and Plastic Surgery, The First Affiliated Hospital of PLA General Hospital, Beijing 100048, P.R. China.
authors.forename: Wan Li
authors.initials: WL
authors.lastname: Chu
authors.affiliation: Department of Burns and Plastic Surgery, The First Affiliated Hospital of PLA General Hospital, Beijing 100048, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Ma
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D053446:CASP8 and FADD-Like Apoptosis Regulating Protein / Q000819:agonists / Q000037:antagonists & inhibitors / Q000235:genetics / Q000276:immunology*; D053148:Caspase 3 / Q000235:genetics / Q000276:immunology; D053181:Caspase 8 / Q000235:genetics / Q000276:immunology; D002470:Cell Survival / Q000187:drug effects; D019610:Cytoprotection; D005312:Fetal Blood / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D005786:Gene Expression Regulation; D059165:Hormesis / Q000276:immunology*; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D061251:Primary Cell Culture; D034741:RNA, Small Interfering / Q000235:genetics / Q000276:immunology; D015398:Signal Transduction
chemical_list: D053446:CASP8 and FADD-Like Apoptosis Regulating Protein; C506386:CFLAR protein, human; D008070:Lipopolysaccharides; D034741:RNA, Small Interfering; C505530:CASP8 protein, human; D053148:Caspase 3; D053181:Caspase 8