title: Helicobacter pylori vesicles carrying CagA localize in the vicinity of cell-cell contacts and induce histone H1 binding to ATP in epithelial cells.
abstract: Helicobacter pylori produces outer membrane vesicles (OMV), delivering bacterial substances including the oncogenic cytotoxin-associated CagA protein to their surroundings. We investigated the effects of H. pylori OMV carrying CagA (OMV-CagA) on cell junctions and ATP-binding proteome of epithelial monolayers, using proteomics, mass spectrometry and imaging. OMV-CagA localized in close vicinity of ZO-1 tight junction protein and induced histone H1 binding to ATP. We suggest the expression of novel events in the interactions between H. pylori OMV and epithelia, which may have an influence on host gene transcription and lead to different outcomes of an infection and development of cancer.
authors.affiliation: Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, SE-58185 Linköping, Sweden.
authors.forename: Maria V
authors.initials: MV
authors.lastname: Turkina
authors.affiliation: Department of Medical Biochemistry and Biophysics, Umeå University, SE-90187 Umeå, Sweden.
authors.forename: Annelie
authors.initials: A
authors.lastname: Olofsson
authors.affiliation: Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, SE-58185 Linköping, Sweden.
authors.forename: Karl-Eric
authors.initials: KE
authors.lastname: Magnusson
authors.affiliation: Department of Medical Biochemistry and Biophysics, Umeå University, SE-90187 Umeå, Sweden.
authors.forename: Anna
authors.initials: A
authors.lastname: Arnqvist
authors.affiliation: Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, SE-58185 Linköping, Sweden elena.vikstrom@liu.se.
authors.forename: Elena
authors.initials: E
authors.lastname: Vikström
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000942:Antigens, Bacterial / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D018938:Caco-2 Cells; D016480:Helicobacter pylori / Q000235:genetics / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D006801:Humans; D018613:Microscopy, Confocal; D019108:Tight Junctions / Q000378:metabolism / Q000648:ultrastructure; D062826:Zonula Occludens-1 Protein / Q000378:metabolism
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D006657:Histones; D062826:Zonula Occludens-1 Protein; C080866:cagA protein, Helicobacter pylori; D000255:Adenosine Triphosphate
keywords: ATP-proteome; CagA; Helicobacter pylori; epithelial cell-cell junctions; histone H1; outer membrane vesicles