title: Identification of CTLA2A, DEFB29, WFDC15B, SERPINA1F and MUP19 as Novel Tissue-Specific Secretory Factors in Mouse.
abstract: Secretory factors in animals play an important role in communication between different cells, tissues and organs. Especially, the secretory factors with specific expression in one tissue may reflect important functions and unique status of that tissue in an organism. In this study, we identified potential tissue-specific secretory factors in the fat, muscle, heart, lung, kidney and liver in the mouse by analyzing microarray data from NCBI's Gene Expression Omnibus (GEO) public repository and searching and predicting their subcellular location in GeneCards and WoLF PSORT, and then confirmed tissue-specific expression of the genes using semi-quantitative PCR reactions. With this approach, we confirmed 11 lung, 7 liver, 2 heart, 1 heart and muscle, 7 kidney and 2 adipose and liver-specific secretory factors. Among these genes, 1 lung-specific gene--CTLA2A (cytotoxic T lymphocyte-associated protein 2 alpha), 3 kidney-specific genes--SERPINA1F (serpin peptidase inhibitor, Clade A, member 1F), WFDC15B (WAP four-disulfide core domain 15B) and DEFB29 (defensin beta 29) and 1 liver-specific gene--MUP19 (major urinary protein 19) have not been reported as secretory factors. These genes were tagged with hemagglutinin at the 3'end and then transiently transfected to HEK293 cells. Through protein detection in cell lysate and media using Western blotting, we verified secretion of the 5 genes and predicted the potential pathways in which they may participate in the specific tissue through data analysis of GEO profiles. In addition, alternative splicing was detected in transcripts of CTLA2A and SERPINA1F and the corresponding proteins were found not to be secreted in cell culture media. Identification of novel secretory factors through the current study provides a new platform to explore novel secretory factors and a general direction for further study of these genes in the future.
authors.affiliation: Department of Animal Sciences, The Ohio State University, Columbus, Ohio, United States of America.
authors.forename: Jibin
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Animal Sciences, The Ohio State University, Columbus, Ohio, United States of America; The Ohio State University Interdisciplinary Ph.D. Program in Nutrition, The Ohio State University, Columbus, Ohio, United States of America.
authors.forename: Jinsoo
authors.initials: J
authors.lastname: Ahn
authors.affiliation: Department of Animal Sciences, The Ohio State University, Columbus, Ohio, United States of America.
authors.forename: Yeunsu
authors.initials: Y
authors.lastname: Suh
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, RDA, Gyeonggi, Republic of Korea.
authors.forename: Seongsoo
authors.initials: S
authors.lastname: Hwang
authors.affiliation: Department of Animal Sciences, The Ohio State University, Columbus, Ohio, United States of America.
authors.forename: Michael E
authors.initials: ME
authors.lastname: Davis
authors.affiliation: Department of Animal Sciences, The Ohio State University, Columbus, Ohio, United States of America.
authors.forename: Kichoon
authors.initials: K
authors.lastname: Lee
mesh_terms: D000818:Animals; D019295:Computational Biology / Q000379:methods*; D030541:Databases, Genetic; D020869:Gene Expression Profiling; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D009928:Organ Specificity; D011506:Proteins / Q000235:genetics* / Q000378:metabolism*; D015843:Serpins / Q000235:genetics / Q000378:metabolism
chemical_list: D011506:Proteins; D015843:Serpins; C035737:major urinary proteins