title: The expression of marker for endometrial stem cell and fibrosis was increased in intrauterine adhesious.
abstract: OBJECTIVE
  The objective of the present study was to evaluate whether fibrotic markers and endometrial stem cell markers were abnormal expressed in endometrium of intrauterine adhesions and a female mouse model for intrauterine adhesions.
  
  
  METHODS
  We revaluated endometrial fibrosis using Masson's stain. We detected the expression of endometrium stem cell markers (CD146 and CD140b) and fibrosis markers (TGF-Beta, CTGF, collagen protein I and collagen protein III) in endometrial tissue with intrauterine adhesions using real-time PCR and S-P (Streptavidin-Peroxidase) immunohistochemistry. We create a female mouse model for intrauterine adhesions using mechanical injury, and then revalue the expression of endometrial stem cell markers and fibrosis markers in endometrial tissue of mouse model for intrauterine adhesions.
  
  
  RESULTS
  The ratio of the area with endometrial fibrosis to total endometrial area in intrauterine adhesious significantly increased compared with the normal endometrial tissue (P < 0.05); The expression levels of fibrotic markers and endometrial stem cell markers were higher in the endometrial tissue with intrauterine adhesious compared to normal endometrial tissue (P < 0.05). The animal experiments showed that the ratio of the area with endometrial fibrosis to total endometrial area significantly increased compared with the control group (P < 0.05); The expression levels of fibrotic markers and endometrial stem cell markers were higher in the endometrial tissue compared to the control group (P < 0.05).
  
  
  CONCLUSIONS
  Aberrant activation of fibrosis may be involved in the pathology of intrauterine adhesious.
authors.affiliation: Department of Obstetrics and Gynecology, Second Affiliated Hospital, Chongqing Medical University Chongqing 400010, China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Hu
authors.affiliation: Department of Obstetrics and Gynecology, Second Affiliated Hospital, Chongqing Medical University Chongqing 400010, China.
authors.forename: Biao
authors.initials: B
authors.lastname: Zeng
authors.affiliation: Department of Obstetrics and Gynecology, Second Affiliated Hospital, Chongqing Medical University Chongqing 400010, China.
authors.forename: Xingwei
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Department of Obstetrics and Gynecology, Second Affiliated Hospital, Chongqing Medical University Chongqing 400010, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Hu
authors.affiliation: Department of Obstetrics and Gynecology, Second Affiliated Hospital, Chongqing Medical University Chongqing 400010, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Meng
authors.affiliation: Department of Obstetrics and Gynecology, Second Affiliated Hospital, Chongqing Medical University Chongqing 400010, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Obstetrics and Gynecology, Second Affiliated Hospital, Chongqing Medical University Chongqing 400010, China.
authors.forename: Min
authors.initials: M
authors.lastname: Mao
mesh_terms: D000328:Adult; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D015153:Blotting, Western; D004195:Disease Models, Animal; D004717:Endometrium / Q000473:pathology*; D005260:Female; D005355:Fibrosis; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D013234:Stem Cells / Q000473:pathology*; D014591:Uterine Diseases / Q000473:pathology*; D014599:Uterus / Q000473:pathology; D055815:Young Adult
chemical_list: D015415:Biomarkers
keywords: Stem cells; fibrosis; intrauterine adhesious