title: Comparison of three magnetic-bead-based RNA extraction methods for detection of cucumber green mottle mosaic virus by real-time RT-PCR.
abstract: To determine the efficiency of RNA extraction methods based on magnetic beads, three different bead-based methods (one using silica-coated magnetic beads [SMNP], one using immunomagnetic beads conjugated to a specific antibody [IMB], and one using magnetic beads to nonspecifically adsorb virions [MNP]) were compared with the TRIzol method for the extraction of cucumber green mottle mosaic virus (CGMMV) RNA from cucumber leaves by real-time RT-PCR. The results indicated that the extraction efficiency of the SMNP method was 10 times higher than those of the IMB and MNP methods and 100 times higher than that of the TRIzol method. Therefore, the SMNP method could be considered for use in quarantine measures for the prevention and control of the disease caused by CGMMV.
authors.affiliation: Beijing Entry-exit Inspection and Quarantine Bureau, Tianshuiyuan Street No.6, Chaoyang District, Beijing, 100026, People's Republic of China, xiaoli9390@163.com.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zhao
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhou
authors.forename: Lijie
authors.initials: L
authors.lastname: Zhang
authors.forename: Wenlong
authors.initials: W
authors.lastname: Yan
authors.forename: Ning
authors.initials: N
authors.lastname: Sun
authors.forename: Xinmiao
authors.initials: X
authors.lastname: Liang
authors.forename: Congliang
authors.initials: C
authors.lastname: Deng
mesh_terms: D018553:Cucumis sativus / Q000821:virology*; D018189:Immunomagnetic Separation / Q000295:instrumentation / Q000379:methods*; D010935:Plant Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*; D017898:Tobamovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D012367:RNA, Viral