title: Effect of NaCl Treatments on Tyramine Biosynthesis of Enterococcus faecalis.
abstract: The effect of NaCl stress (0 to 8%, wt/vol) on the growth and tyramine production in two Enterococcus faecalis strains was examined during culture time. The growth of E. faecalis was inhibited by the increase in NaCl concentration, but tyramine production was unaffected. Tyramine accumulated rapidly during the logarithmic phase of the strains, and the final tyramine levels were approximately 800 μg/ml. Relative gene expression of four genes in the tyrosine decarboxylase locus, namely, tyrRS, tyrDC, tyrP, and nhaC, was evaluated at different incubation times. The results showed that NaCl stress could upregulate the expression of tyrDC and tyrP to improve the tyramine production of a single E. faecalis strain under certain conditions, and TyrS could act as a negative regulator on the genetic regulation of the tyramine cluster.
authors.affiliation: Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, People's Republic of China; College of Food Science, Northeast Agricultural University, Harbin 150030, People's Republic of China.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.affiliation: College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023, People's Republic of China.
authors.forename: Xinxin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, People's Republic of China.
authors.forename: Lihui
authors.initials: L
authors.lastname: Du
authors.affiliation: Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, People's Republic of China.
authors.forename: Daoying
authors.initials: D
authors.lastname: Wang
authors.affiliation: Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, People's Republic of China.
authors.forename: Yongzhi
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, People's Republic of China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Geng
authors.affiliation: College of Food Science, Northeast Agricultural University, Harbin 150030, People's Republic of China. 29520002@qq.com.
authors.forename: Xiaoxi
authors.initials: X
authors.lastname: Xu
authors.affiliation: Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, People's Republic of China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Xu
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D013293:Enterococcus faecalis / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D012965:Sodium Chloride / Q000378:metabolism*; D014439:Tyramine / Q000096:biosynthesis*; D014445:Tyrosine Decarboxylase / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D012965:Sodium Chloride; D014445:Tyrosine Decarboxylase; D014439:Tyramine