title: Co-culture of annulus fibrosus cells and bone marrow mesenchymal stem cells.
abstract: To investigate the effect on cell proliferation and extracellular matrix expression of annulus fibrosus (AF) cells when co-cultured with bone marrow mesenchymal stem cells (BMSCs). Primary isolated rabbit BMSCs and AF cells were cultured and harvested cells were placed into a 15-mL centrifugal tube co-culture system in a ratio of 2:1 (A group), 1:1 (B group), and 1:2 (C group). Cell proliferation was evaluated using cell counting kit-8, and mRNA of collagen II and mucopolysaccharide was quantified using real-time polymerase chain reaction (PCR) on Days 7, 14, and 21. The cell count, synthesized collagen II and mucopolysaccharide increased in a time-dependent manner, with a peak at Day 21. Cells in Group B proliferated faster and synthesized more collagen II and mucopolysaccharide than groups A and C, where the difference was significant. AF cells and BMSCs in the ratio of 1:1, cultured using the centrifugal tube three-dimensional co-culture system showed that BMSCs can promote AF cell proliferation and extracellular matrix synthesis.
authors.affiliation: Department of Orthopedics, West Hospital of Jinan Military General Hospital, Shandong, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Orthopedics, West Hospital of Jinan Military General Hospital, Shandong, China.
authors.forename: X J
authors.initials: XJ
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, Central Hospital of Shengli Oilfield, Dongying, Shandong, China.
authors.forename: L
authors.initials: L
authors.lastname: Fang
authors.affiliation: Department of Orthopedics, West Hospital of Jinan Military General Hospital, Shandong, China TingbaoZhao@163.com.
authors.forename: T B
authors.initials: TB
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D049109:Cell Proliferation; D018920:Coculture Techniques; D024043:Collagen Type II / Q000235:genetics; D006025:Glycosaminoglycans / Q000235:genetics; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D024043:Collagen Type II; D006025:Glycosaminoglycans; D012333:RNA, Messenger