title: Sulfated Polysaccharides Isolated from Cloned Grateloupia filicina and Their Anticoagulant Activity.
abstract: Sulfated polysaccharides (GSP) were isolated from the cloned Grateloupia filicina which was cultured in Jiaozhou Bay, Qingdao, China. The yield of GSP was 15.75%. The total sugar and sulfate were 40.90 and 19.89%, respectively. And the average molecular weight was 11.7 KDa. The results of neutral sugar analysis showed that GSP was mainly sulfated polysaccharides of galactose. The experiments for activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) anticoagulant assays in vitro indicated that GSP was a good potential anticoagulant. Therefore, this study supplied new thought for the cloned Grateloupia filicina exploitation of high-value products.
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, No. 7 Nanhai Road, Qingdao, Shandong 266071, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Chen
authors.affiliation: Qingdao Tumor Hospital, No. 127 Siliu Nan Road, Qingdao, Shandong 266042, China.
authors.forename: Shengfeng
authors.initials: S
authors.lastname: Yang
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, No. 7 Nanhai Road, Qingdao, Shandong 266071, China.
authors.forename: Jinxia
authors.initials: J
authors.lastname: Wang
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, No. 7 Nanhai Road, Qingdao, Shandong 266071, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Song
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, No. 7 Nanhai Road, Qingdao, Shandong 266071, China.
authors.forename: Ronge
authors.initials: R
authors.lastname: Xing
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, No. 7 Nanhai Road, Qingdao, Shandong 266071, China.
authors.forename: Song
authors.initials: S
authors.lastname: Liu
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, No. 7 Nanhai Road, Qingdao, Shandong 266071, China.
authors.forename: Huahua
authors.initials: H
authors.lastname: Yu
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, No. 7 Nanhai Road, Qingdao, Shandong 266071, China.
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Li
mesh_terms: D000925:Anticoagulants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001780:Blood Coagulation Tests; D002681:China; D005690:Galactose / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D010314:Partial Thromboplastin Time; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011517:Prothrombin Time; D000461:Rhodophyta / Q000737:chemistry*; D013431:Sulfates / Q000737:chemistry; D013918:Thrombin Time
chemical_list: D000925:Anticoagulants; D011134:Polysaccharides; D013431:Sulfates; D005690:Galactose