title: Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines.
abstract: Exosomes are important mediators in cell-to-cell communication and, recently, their role in melanoma progression has been brought to light. Here, we characterized exosomes secreted by seven melanoma cell lines with varying degrees of aggressivity. Extensive proteomic analysis of their exosomes confirmed the presence of characteristic exosomal markers as well as melanoma-specific antigens and oncogenic proteins. Importantly, the protein composition differed among exosomes from different lines. Exosomes from aggressive cells contained specific proteins involved in cell motility, angiogenesis, and immune response, while these proteins were less abundant or absent in exosomes from less aggressive cells. Interestingly, when exposed to exosomes from metastatic lines, less aggressive cells increased their migratory capacities, likely due to transfer of pro-migratory exosomal proteins to recipient cells. Hence, this study shows that the specific protein composition of melanoma exosomes depends on the cells' aggressivity and suggests that exosomes influence the behavior of other tumor cells and their microenvironment.
authors.affiliation: CNRS UMR 5089, Institut de Pharmacologie et de Biologie Structurale, Toulouse, France.|UPS, Université de Toulouse, Toulouse, France.
authors.forename: Ikrame
authors.initials: I
authors.lastname: Lazar
authors.affiliation: CNRS UMR 5089, Institut de Pharmacologie et de Biologie Structurale, Toulouse, France.|UPS, Université de Toulouse, Toulouse, France.
authors.forename: Emily
authors.initials: E
authors.lastname: Clement
authors.affiliation: CNRS UMR 5089, Institut de Pharmacologie et de Biologie Structurale, Toulouse, France.|UPS, Université de Toulouse, Toulouse, France.
authors.forename: Manuelle
authors.initials: M
authors.lastname: Ducoux-Petit
authors.affiliation: UMR 3347 CNRS, U1021 INSERM, Institut Curie, Ligue nationale contre le cancer (Equipe labellisée), Orsay, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Denat
authors.affiliation: Bat IBCG, Plateforme de Microscopie électronique intégrative (METi), FRBT CNRS FR3451, Toulouse, France.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Soldan
authors.affiliation: CNRS UMR 5089, Institut de Pharmacologie et de Biologie Structurale, Toulouse, France.|UPS, Université de Toulouse, Toulouse, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Dauvillier
authors.affiliation: Bat IBCG, Plateforme de Microscopie électronique intégrative (METi), FRBT CNRS FR3451, Toulouse, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Balor
authors.affiliation: CNRS UMR 5089, Institut de Pharmacologie et de Biologie Structurale, Toulouse, France.|UPS, Université de Toulouse, Toulouse, France.
authors.forename: Odile
authors.initials: O
authors.lastname: Burlet-Schiltz
authors.affiliation: UMR 3347 CNRS, U1021 INSERM, Institut Curie, Ligue nationale contre le cancer (Equipe labellisée), Orsay, France.
authors.forename: Lionel
authors.initials: L
authors.lastname: Larue
authors.affiliation: CNRS UMR 5089, Institut de Pharmacologie et de Biologie Structurale, Toulouse, France.|UPS, Université de Toulouse, Toulouse, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Muller
authors.affiliation: CNRS UMR 5089, Institut de Pharmacologie et de Biologie Structurale, Toulouse, France.|UPS, Université de Toulouse, Toulouse, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Nieto
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D055354:Exosomes / Q000378:metabolism*; D006801:Humans; D013058:Mass Spectrometry; D008545:Melanoma / Q000378:metabolism*; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics
chemical_list: D009363:Neoplasm Proteins; D020543:Proteome
keywords: biomarker; cancer; exosome; extracellular vesicles; melanoma; metastasis; proteome