title: Detection of a novel herpesvirus from bats in the Philippines.
abstract: Bats are natural hosts of many zoonotic viruses. Monitoring bat viruses is important to detect novel bat-borne infectious diseases. In this study, next generation sequencing techniques and conventional PCR were used to analyze intestine, lung, and blood clot samples collected from wild bats captured at three locations in Davao region, in the Philippines in 2012. Different viral genes belonging to the Retroviridae and Herpesviridae families were identified using next generation sequencing. The existence of herpesvirus in the samples was confirmed by PCR using herpesvirus consensus primers. The nucleotide sequences of the resulting PCR amplicons were 166-bp. Further phylogenetic analysis identified that the virus from which this nucleotide sequence was obtained belonged to the Gammaherpesvirinae subfamily. PCR using primers specific to the nucleotide sequence obtained revealed that the infection rate among the captured bats was 30 %. In this study, we present the partial genome of a novel gammaherpesvirus detected from wild bats. Our observations also indicate that this herpesvirus may be widely distributed in bat populations in Davao region.
authors.affiliation: Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Saiwai, Fuchu, Tokyo, 183-8509, Japan.
authors.forename: Kaori
authors.initials: K
authors.lastname: Sano
authors.forename: Sachiko
authors.initials: S
authors.lastname: Okazaki
authors.forename: Satoshi
authors.initials: S
authors.lastname: Taniguchi
authors.forename: Joseph S
authors.initials: JS
authors.lastname: Masangkay
authors.forename: Roberto
authors.initials: R
authors.lastname: Puentespina
authors.forename: Eduardo
authors.initials: E
authors.lastname: Eres
authors.forename: Edison
authors.initials: E
authors.lastname: Cosico
authors.forename: Niña
authors.initials: N
authors.lastname: Quibod
authors.forename: Taisuke
authors.initials: T
authors.lastname: Kondo
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shimoda
authors.forename: Yuuki
authors.initials: Y
authors.lastname: Hatta
authors.forename: Shumpei
authors.initials: S
authors.lastname: Mitomo
authors.forename: Mami
authors.initials: M
authors.lastname: Oba
authors.forename: Yukie
authors.initials: Y
authors.lastname: Katayama
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Sassa
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Furuya
authors.forename: Makoto
authors.initials: M
authors.lastname: Nagai
authors.forename: Yumi
authors.initials: Y
authors.lastname: Une
authors.forename: Ken
authors.initials: K
authors.lastname: Maeda
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kyuwa
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Yoshikawa
authors.forename: Hiroomi
authors.initials: H
authors.lastname: Akashi
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Omatsu
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Mizutani
mesh_terms: D000818:Animals; D002685:Chiroptera / Q000821:virology*; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics*; D018142:Gammaherpesvirinae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D006566:Herpesviridae Infections / Q000662:veterinary* / Q000821:virology; D008969:Molecular Sequence Data; D010679:Philippines; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D004279:DNA, Viral