title: Inhibitory Effect of Fluoride on Na+,K+ ATPase Activity in Human Erythrocyte Membrane.
abstract: The present study was performed to evaluate the role of long-term consumption of excessive fluoride on electrolyte homeostasis and their transporting mechanisms in erythrocytes of subjects afflicted with dental and skeletal fluorosis. A total of 620 adult (20-50 years) Indian residents participated in this study: 258 men and 242 women exposed to high concentrations of fluoride and 120 age and gender-matched control subjects. Erythrocytes were isolated from blood samples, washed, and used for the estimation of intraerythrocyte sodium and potassium concentrations. Na+,K+ ATPase activity was determined spectrophotometrically from a ghost erythrocyte membrane prepared by osmotic lysis. Erythrocyte analytes were correlated with the water and serum fluoride concentrations by Pearson's bivariate correlation and regression analysis. Results indicated a significant increase in intraerythrocyte sodium (F=14306.265, P<0.0001) in subjects from endemic fluorosis study groups as compared to controls. A significant (P<0.05) positive correlation of intracellular sodium was found with water and serum fluoride concentrations. Mean concentration of intraerythrocytic potassium ions showed significant reduction (F=9136.318, P<0.0001) in subjects exposed to fluoride. A significant (P<0.05) negative correlation of potassium ions was noted with water and serum fluoride concentrations. Na+,K+ ATPase activity was significantly declined (F=1572.763, P<0.0001) in subjects exposed to fluoride. A significant (P<0.05) inverse relationship of Na+,K+ ATPase activity was revealed with water and serum fluoride concentrations.
authors.affiliation: Department of Zoology and Environmental Sciences, Punjabi University, Patiala, 147002, Punjab, India. shashiuniindia@yahoo.co.in.
authors.forename: Shashi
authors.initials: S
authors.lastname: A
authors.affiliation: Department of Zoology and Environmental Sciences, Punjabi University, Patiala, 147002, Punjab, India.
authors.forename: Meenakshi
authors.initials: M
authors.lastname: G
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D004573:Electrolytes; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000201:enzymology*; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology*; D005260:Female; D005459:Fluorides / Q000737:chemistry*; D006706:Homeostasis; D006801:Humans; D017136:Ion Transport; D008297:Male; D008875:Middle Aged; D009995:Osmosis; D010710:Phosphates / Q000737:chemistry*; D011188:Potassium / Q000032:analysis; D012964:Sodium / Q000032:analysis; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*; D055815:Young Adult
chemical_list: D004573:Electrolytes; D010710:Phosphates; C012980:fluorophosphate; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D005459:Fluorides; D011188:Potassium
keywords: Erythrocyte membrane; Fluoride; Intracellular potassium and sodium ions; Na+,K+ ATPase activity