title: Expression and Purification of RNA-Protein Complexes in Escherichia coli.
abstract: For structural, biochemical or pharmacological studies, it is required to have pure RNA in large quantities. We previously devised a generic approach that allows efficient in vivo expression of recombinant RNA in Escherichia coli. We have extended the "tRNA scaffold" method to RNA/protein co-expression in order to express and purify RNA by affinity in native condition. As a proof-of-concept, we present the expression and the purification of the AtRNA-mala in complex with the MS2 coat protein.
authors.affiliation: Center for Molecular OncologyBarts Cancer Institute, Queen Mary University of London, London, UK.
authors.forename: Margueritte
authors.initials: M
authors.lastname: El Khouri
authors.forename: Marjorie
authors.initials: M
authors.lastname: Catala
authors.forename: Bili
authors.initials: B
authors.lastname: Seijo
authors.forename: Johana
authors.initials: J
authors.lastname: Chabal
authors.forename: Carine
authors.initials: C
authors.lastname: Tisné
authors.forename: Frédéric
authors.initials: F
authors.lastname: Dardel
authors.forename: Luc
authors.initials: L
authors.lastname: Ponchon
mesh_terms: D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D015870:Gene Expression; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D011506:Proteins / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D012313:RNA / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D012343:RNA, Transfer / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D011506:Proteins; D012313:RNA; D012343:RNA, Transfer