title: Coencapsulation of hydrophobic and hydrophilic antituberculosis drugs in synergistic Brij 96 microemulsions: a biophysical characterization.
abstract: A microemulsion has been formulated to coencapsulate antituberculosis drugs to solve the issue of stability of rifampicin (RIF) in the presence of isoniazid (INH) and pyrazinamide (PZA). The structural transition, solubilization locus, and quantitative release of drugs without interference have been estimated. Derivative absorbance spectroscopy, especially ratio derivative and double divisor ratio derivative methods, has been employed for estimating the release. The coencapsulation of the anti-tuberculosis drugs were carried out in single, binary, or ternary mixtures and occupy the same solubilization sites in multiple drugs microemulsion systems as in the case of single drug-loaded systems. INH and PZA obey the diffusional (Fickian) release mechanism, whereas RIF shows anomalous release. Resazurin assay and agar well diffusion method were adopted for cytotoxicity analysis and antimicrobial activity, respectively. Cytotoxicity was found to be dependent on concentration and on colloidal structure of microemulsion.
authors.affiliation: Department of Chemistry and Centre of Advanced Studies in Chemistry, Panjab University, Chandigarh, 160014, Punjab, India.
authors.forename: Gurpreet
authors.initials: G
authors.lastname: Kaur
authors.affiliation: Department of Chemistry and Centre of Advanced Studies in Chemistry, Panjab University, Chandigarh, 160014, Punjab, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Mehta
authors.affiliation: Department of Bio and Nano Technology, Guru Jambheshwar University of Science and Technology, Hisar, 125001, Haryana, India.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Department of Bio and Nano Technology, Guru Jambheshwar University of Science and Technology, Hisar, 125001, Haryana, India.
authors.forename: Gaurav
authors.initials: G
authors.lastname: Bhanjana
authors.affiliation: Department of Bio and Nano Technology, Guru Jambheshwar University of Science and Technology, Hisar, 125001, Haryana, India.
authors.forename: Neeraj
authors.initials: N
authors.lastname: Dilbaghi
mesh_terms: D000818:Animals; D000995:Antitubercular Agents / Q000737:chemistry*; D002460:Cell Line; D002626:Chemistry, Pharmaceutical / Q000379:methods; D002522:Chlorocebus aethiops; D004655:Emulsions / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D007538:Isoniazid / Q000737:chemistry; D010078:Oxazines / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011718:Pyrazinamide / Q000737:chemistry; D012293:Rifampin / Q000737:chemistry; D012995:Solubility; D014709:Vero Cells; D014966:Xanthenes / Q000737:chemistry
chemical_list: D000995:Antitubercular Agents; D004655:Emulsions; D010078:Oxazines; D010938:Plant Oils; D014966:Xanthenes; C005843:resazurin; D011718:Pyrazinamide; D011092:Polyethylene Glycols; C033084:polyethylene glycol oleyl ether; D007538:Isoniazid; D012293:Rifampin
keywords: Brij 96; UV-visible spectroscopy; anti-TB drugs; derivative absorbance spectroscopy; in vitro release; nonionic microemulsions; preformulation; solubility; stability; toxicity