title: Normalization of Affymetrix miRNA Microarrays for the Analysis of Cancer Samples.
abstract: microRNA (miRNA) microarray normalization is a critical step for the identification of truly differentially expressed miRNAs. This is particularly important when dealing with cancer samples that have a global miRNA decrease. In this chapter, we provide a simple step-by-step procedure that can be used to normalize Affymetrix miRNA microarrays, relying on robust normal-exponential background correction with cyclic loess normalization.
authors.affiliation: Department of Statistics, Harvard University, Cambridge, MA, USA.|Centre for Cancer Research, MIMR-PHI Institute of Medical Research, 27-31 Wright Street, Clayton, VIC, 3168, Australia.
authors.forename: Di
authors.initials: D
authors.lastname: Wu
authors.affiliation: Centre for Cancer Research, MIMR-PHI Institute of Medical Research, 27-31 Wright Street, Clayton, VIC, 3168, Australia. michael.gantier@mimr-phi.org.|Department of Molecular and Translational Science, Monash University, Clayton, VIC, Australia. michael.gantier@mimr-phi.org.
authors.forename: Michael P
authors.initials: MP
authors.lastname: Gantier
mesh_terms: D019295:Computational Biology / Q000379:methods* / Q000592:standards; D023281:Genomics / Q000379:methods* / Q000592:standards; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009369:Neoplasms / Q000175:diagnosis / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods* / Q000592:standards; D011933:Reagent Kits, Diagnostic / Q000592:standards; D012984:Software
chemical_list: D035683:MicroRNAs; D011933:Reagent Kits, Diagnostic
keywords: Affymetrix; Cancer samples; Normalization; miRNA microarray; microRNA