title: miR-24-2 regulates genes in survival pathway and demonstrates potential in reducing cellular viability in combination with docetaxel.
abstract: MicroRNAs the small (18-22 in length) noncoding RNA molecules are negative regulators of gene expression, modulating biological processes of cell differentiation, survival and death. The latter two phenomena are critical in tumour biology. We provide here the results of human genome wide target prediction of one such microRNA, hsa-miR-24-2, shown to target genes essential for initiating cellular stability and cell survival. The protein-protein interaction study showed important nodes which could affect cell cycle progression and differential oncogenesis. An analysis of hsa-miR-24-2 in sporadic breast tumours showed a negative correlation with metastasis and increasing nodes. The conclusion drawn of hsa-miR-24-2 targeting the genes of cell survival correlated with the methylation profile and resultant transcription factor binding site gain or loss in support of absence of cell survival. In order to accentuate the potential of hsa-miR-24-2 to reduce cellular viability under experimental conditions, in vitro studies in the presence and absence of anti-cancer drugs, such as docetaxel resulted in a significant decrease in cellular viability even at a 200-fold reduced dose of the drug in combination with hsa-miR-24-2.
authors.affiliation: School of Biotechnology, Shri Mata Vaishno Devi University, Katra, Kakrayal, Jammu & Kashmir, India. Electronic address: rahulmanvati@gmail.com.
authors.forename: Siddharth
authors.initials: S
authors.lastname: Manvati
authors.affiliation: National Centre of Applied Human Genetics, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India. Electronic address: kailashmangalhara21@gmail.com.
authors.forename: Kailash Chandra
authors.initials: KC
authors.lastname: Mangalhara
authors.affiliation: School of Biotechnology, Shri Mata Vaishno Devi University, Katra, Kakrayal, Jammu & Kashmir, India. Electronic address: kalaiinfo@gmail.com.
authors.forename: P
authors.initials: P
authors.lastname: Kalaiarasan
authors.affiliation: National Centre of Applied Human Genetics, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India. Electronic address: srivastavaniloo@gmail.com.
authors.forename: Niloo
authors.initials: N
authors.lastname: Srivastava
authors.affiliation: National Centre of Applied Human Genetics, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India. Electronic address: bamezai@hotmail.com.
authors.forename: R N K
authors.initials: RN
authors.lastname: Bamezai
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics*; D000077143:Docetaxel; D019008:Drug Resistance, Neoplasm; D015870:Gene Expression; D056945:Hep G2 Cells; D006801:Humans; D061986:MCF-7 Cells; D035683:MicroRNAs / Q000502:physiology*; D060066:Protein Interaction Maps; D034622:RNA Interference; D043823:Taxoids / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; C543845:MIRN24 microRNA, human; D035683:MicroRNAs; D043823:Taxoids; D000077143:Docetaxel
keywords: Apoptosis; Cell death; Docetaxel; MicroRNA; miR-24-2