title: Mechanism of the effect of glycosyltransferase GLT8D2 on fatty liver.
abstract: BACKGROUND
  Recent studies have shown that some glycosyltransferases are involved in the development of nonalcoholic fatty liver disease (NAFLD). The objective of this study was to explore the effect and mechanism of glycosyltransferase GLT8D2 on fatty liver.
  
  
  METHODS
  Rat model of NAFLD was established by induction with high-fat-diet. The GLT8D2 expression in rat liver was examined using immunohistochemistry. Oil Red O staining and triglyceride assay were used to measure the effect of abnormal GLT8D2 expression on lipid accumulation in HepG2 cells. The expression levels of lipid metabolism-related key molecules, namely sterol regulatory element-binding protein-1c (SREBP-1c), stearoyl-coA desaturase (SCD), carnitine palmitoyltransferase-1 (CPT1) and microsomal triglyceride transfer protein (MTP), in HepG2 cells with abnormal GLT8D2 expression were determined by western blot analyses.
  
  
  RESULTS
  The expression of GLT8D2 was higher in the liver of rats with NAFLD than in the control rats, and GLT8D2 was mainly located around lipid droplets in hepatocytes. GLT8D2 expression increased in steatosis HepG2 cells compared with that in normal HepG2 cells. GLT8D2 positively regulated lipid droplet accumulation and triglyceride content in HepG2 cells. Upregulation or knockdown of GLT8D2 had no effect on the expressions of SREBP-1c, SCD or CPT-1 proteins in HepG2 cells. However, GLT8D2 expression negatively regulated the expression of MTP protein in HepG2 cells.
  
  
  CONCLUSIONS
  GLT8D2 participated in NAFLD pathogenesis possibly by negatively regulating MTP expression. Specific inhibition of GLT8D2 via an antagonistic strategy could provide a potential candidate approach for treatment of NAFLD.
authors.affiliation: Department of Gastroenterology, Beijing Tongren Hospital, Capital Medical University, No.1 Dongjiaominxiang, Dongcheng District, Beijing, 100730, China. yutaozhan@263.net.
authors.forename: Yutao
authors.initials: Y
authors.lastname: Zhan
authors.affiliation: Department of Gastroenterology, Beijing Tongren Hospital, Capital Medical University, No.1 Dongjiaominxiang, Dongcheng District, Beijing, 100730, China. fayefly.future@163.com.
authors.forename: Fei
authors.initials: F
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing, 102206, China. xpxp922@163.com.
authors.forename: Ping
authors.initials: P
authors.lastname: Xie
authors.affiliation: Department of Gastroenterology, Beijing Tongren Hospital, Capital Medical University, No.1 Dongjiaominxiang, Dongcheng District, Beijing, 100730, China. zhongleping@gmail.com.
authors.forename: Leping
authors.initials: L
authors.lastname: Zhong
authors.affiliation: Department of Gastroenterology, Beijing Tongren Hospital, Capital Medical University, No.1 Dongjiaominxiang, Dongcheng District, Beijing, 100730, China. li019985@163.com.
authors.forename: Dongnian
authors.initials: D
authors.lastname: Li
authors.affiliation: Institutes of Infectious Disease, Beijing Ditan Hospital, Capital Medical University, Beijing, 100015, China. gaiqujing@yeah.net.
authors.forename: Qujing
authors.initials: Q
authors.lastname: Gai
authors.affiliation: Department of Gastroenterology, Beijing Tongren Hospital, Capital Medical University, No.1 Dongjiaominxiang, Dongcheng District, Beijing, 100730, China. doctorlili008@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Institutes of Infectious Disease, Beijing Ditan Hospital, Capital Medical University, Beijing, 100015, China. drliver@163.com.
authors.forename: Hongshan
authors.initials: H
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing, 102206, China. zhanglq@nic.bmi.ac.cn.
authors.forename: Lingqiang
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Cell Biology, Municipal Laboratory for Liver Protection and Regulation of Regeneration, Capital Medical University, 10 You An Men Wai Xi Tou Tiao, Beijing, 100069, China. anwei@ccmu.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: An
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002352:Carrier Proteins / Q000378:metabolism; D004195:Disease Models, Animal; D056945:Hep G2 Cells / Q000737:chemistry / Q000201:enzymology; D006801:Humans; D008055:Lipids / Q000032:analysis; D008297:Male; D065626:Non-alcoholic Fatty Liver Disease / Q000201:enzymology* / Q000209:etiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014280:Triglycerides / Q000032:analysis / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D008055:Lipids; D014280:Triglycerides; C106092:microsomal triglyceride transfer protein