title: Preparation of a poly(3'-azido-3'-deoxythymidine-co-propargyl methacrylate-co-pentaerythritol triacrylate) monolithic column by in situ polymerization and a click reaction for capillary liquid chromatography of small molecules and proteins.
abstract: Combining free radical polymerization with click chemistry via a copper-mediated azide/alkyne cycloaddition (CuAAC) reaction in a "one-pot" process, a facile approach was developed for the preparation of a poly(3'-azido-3'-deoxythymidine-co-propargyl methacrylate-co-pentaerythritol triacrylate) (AZT-co-PMA-co-PETA) monolithic column. The resulting poly(AZT-co-PMA-co-PETA) monolith showed a relatively homogeneous monolithic structure, good permeability and mechanical stability. Different ratios of monomers and porogens were used for optimizing the properties of a monolithic column. A series of alkylbenzenes, amides, anilines, and benzoic acids were used to evaluate the chromatographic properties of the polymer monolith in terms of hydrophobic, hydrophilic and cation-exchange interactions, and the results showed that the poly(AZT-co-PMA-co-PETA) monolith exhibited more flexible adjustment in chromatographic selectivity than that of the parent poly(PMA-co-PETA) and AZT-modified poly(PMA-co-PETA) monoliths. Column efficiencies for toluene, DMF, and formamide with 35,000-48,000 theoretical plates per m could be obtained at a linear velocity of 0.17 mm s(-1). The run-to-run, column-to-column, and batch-to-batch repeatabilities of the retention factors were less than 4.2%. In addition, the proposed monolith was also applied to efficient separation of sulfonamides, nucleobases and nucleosides, anesthetics and proteins for demonstrating its potential.
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350116, China.
authors.forename: Zian
authors.initials: Z
authors.lastname: Lin
authors.forename: Ruifang
authors.initials: R
authors.lastname: Yu
authors.forename: Wenli
authors.initials: W
authors.lastname: Hu
authors.forename: Jiangnan
authors.initials: J
authors.lastname: Zheng
authors.forename: Ping
authors.initials: P
authors.lastname: Tong
authors.forename: Hongzhi
authors.initials: H
authors.lastname: Zhao
authors.forename: Zongwei
authors.initials: Z
authors.lastname: Cai
mesh_terms: D000179:Acrylates / Q000737:chemistry*; D000480:Alkynes / Q000737:chemistry; D000777:Anesthetics / Q000302:isolation & purification; D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification; D001386:Azides / Q000737:chemistry; D002384:Catalysis; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase; D057930:Click Chemistry; D003300:Copper / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D007474:Ion Exchange; D009705:Nucleosides / Q000302:isolation & purification; D058105:Polymerization; D011108:Polymers / Q000737:chemistry*; D011409:Propylene Glycols / Q000737:chemistry*; D011506:Proteins / Q000302:isolation & purification*; D013449:Sulfonamides / Q000302:isolation & purification; D015215:Zidovudine / Q000737:chemistry*
chemical_list: D000179:Acrylates; D000480:Alkynes; D000777:Anesthetics; D000900:Anti-Bacterial Agents; D001386:Azides; D009705:Nucleosides; D011108:Polymers; D011409:Propylene Glycols; D011506:Proteins; D013449:Sulfonamides; D015215:Zidovudine; D003300:Copper; C027992:pentaerythrityl triacrylate