title: Determination of Multimodal Isotopic Distributions: The Case of a (15)N Labeled Protein Produced into Hairy Roots.
abstract: Isotopic labeling is widely used in various fields like proteomics, metabolomics, fluxomics, as well as in NMR structural studies, but it requires an efficient determination of the isotopic enrichment. Mass spectrometry is the method of choice for such analysis. However, when complex expression systems like hairy roots are used for production, multiple populations of labeled proteins may be obtained. If the isotopic incorporation determination is actually well-known for unimodal distributions, the multimodal distributions have scarcely been investigated. Actually, only a few approaches allow the determination of the different labeled population proportions from multimodal distributions. Furthermore, they cannot be used when the number of the populations and their respective isotope ratios are unknown. The present study implements a new strategy to measure the (15)N labeled populations inside a multimodal distribution knowing only the peptide sequence and peak intensities from mass spectrometry analyses. Noteworthy, it could be applied to other elements, like carbon and hydrogen, and extended to a larger range of biomolecules.
authors.affiliation: †Normandie Université, COBRA, UMR6014 and IRIB; Université de Rouen; INSA de Rouen; CNRS, IRCOF, 1 rue Tesnière, 76821 Mont-Saint-Aignan Cedex, France.
authors.forename: Romain
authors.initials: R
authors.lastname: Trouillard
authors.affiliation: ‡Normandie Université, COBRA, UMR6014 and FR3038; Université de Rouen; INSA de Rouen; CNRS, IRCOF, 1 rue Tesnière, 76821 Mont-Saint-Aignan Cedex, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Hubert-Roux
authors.affiliation: ‡Normandie Université, COBRA, UMR6014 and FR3038; Université de Rouen; INSA de Rouen; CNRS, IRCOF, 1 rue Tesnière, 76821 Mont-Saint-Aignan Cedex, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Tognetti
authors.affiliation: †Normandie Université, COBRA, UMR6014 and IRIB; Université de Rouen; INSA de Rouen; CNRS, IRCOF, 1 rue Tesnière, 76821 Mont-Saint-Aignan Cedex, France.
authors.forename: Laure
authors.initials: L
authors.lastname: Guilhaudis
authors.affiliation: §Normandie Université, Glyco-MEV, EA 4358 and IRIB, Université de Rouen, 1 rue Tesnière, 76821 Mont-Saint-Aignan Cedex, France.
authors.forename: Carole
authors.initials: C
authors.lastname: Plasson
authors.affiliation: §Normandie Université, Glyco-MEV, EA 4358 and IRIB, Université de Rouen, 1 rue Tesnière, 76821 Mont-Saint-Aignan Cedex, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Menu-Bouaouiche
authors.affiliation: ∥CNRS UMR 6270, PBS, Plateforme Protéomique PISSARO, IRIB, FR3038 INC3M, Normandie Université, Université de Rouen, Boulevard Maurice de Broglie, 76821 Mont-Saint-Aignan Cedex, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Coquet
authors.affiliation: ⊥Biologie des plantes et innovation (BioPI), Université de Picardie Jules Verne, 33 rue St Leu, 80039 Amiens, France.
authors.forename: François
authors.initials: F
authors.lastname: Guerineau
authors.affiliation: ∥CNRS UMR 6270, PBS, Plateforme Protéomique PISSARO, IRIB, FR3038 INC3M, Normandie Université, Université de Rouen, Boulevard Maurice de Broglie, 76821 Mont-Saint-Aignan Cedex, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Hardouin
authors.affiliation: ⊥Biologie des plantes et innovation (BioPI), Université de Picardie Jules Verne, 33 rue St Leu, 80039 Amiens, France.
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Ele Ekouna
authors.affiliation: ∥CNRS UMR 6270, PBS, Plateforme Protéomique PISSARO, IRIB, FR3038 INC3M, Normandie Université, Université de Rouen, Boulevard Maurice de Broglie, 76821 Mont-Saint-Aignan Cedex, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Cosette
authors.affiliation: §Normandie Université, Glyco-MEV, EA 4358 and IRIB, Université de Rouen, 1 rue Tesnière, 76821 Mont-Saint-Aignan Cedex, France.
authors.forename: Patrice
authors.initials: P
authors.lastname: Lerouge
authors.affiliation: ⊥Biologie des plantes et innovation (BioPI), Université de Picardie Jules Verne, 33 rue St Leu, 80039 Amiens, France.
authors.forename: Michèle
authors.initials: M
authors.lastname: Boitel-Conti
authors.affiliation: ‡Normandie Université, COBRA, UMR6014 and FR3038; Université de Rouen; INSA de Rouen; CNRS, IRCOF, 1 rue Tesnière, 76821 Mont-Saint-Aignan Cedex, France.
authors.forename: Carlos
authors.initials: C
authors.lastname: Afonso
authors.affiliation: †Normandie Université, COBRA, UMR6014 and IRIB; Université de Rouen; INSA de Rouen; CNRS, IRCOF, 1 rue Tesnière, 76821 Mont-Saint-Aignan Cedex, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Ségalas-Milazzo
mesh_terms: D029741:Brassica rapa / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000032:analysis*; D006801:Humans; D013058:Mass Spectrometry; D009587:Nitrogen Isotopes; D018517:Plant Roots / Q000737:chemistry*
chemical_list: D009587:Nitrogen Isotopes; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins