title: Effects of silver nanoparticles and gold nanoparticles on IL-2, IL-6, and TNF-α production via MAPK pathway in leukemic cell lines.
abstract: Silver nanopaticles (AgNPs) and gold nanoparticles (AuNPs) have various applications in medical healthcare and various biological properties such as anti-inflammation, anti-cancer, and anti-angiogenesis. In this study, we investigated the effect of AgNPs and AuNPs on cytokine production via the mitogen-activated protein kinase pathway in leukemic cell lines (T-lymphocytic Jurkat and monocytic U937 cells). We found that both AgNPs and AuNPs inhibited cell proliferation of leukemic cell lines. AgNPs inhibited TNF-α, while AuNPs inhibited interleukin-2 production in Jurkat cells, in which inhibition of cytokines is involved in the extracellular-signal regulated protein kinase but not the c-Jun N-terminal kinase pathway. In U937 cells, AuNPs inhibited interleukin-6 but stimulated TNF-α production in a concentration-dependent manner through the c-Jun N-terminal kinase but not the extracellular-signal regulated protein kinase pathway. Our study showed that each leukemic cell line treated with nanoparticles exhibited a distinct signaling pathway response to inhibit or stimulate cytokine production, leading to anti-cell proliferation. The effects of AgNPs and AuNPs on leukemic cell lines may have a significant impact on leukemia treatment in the future.
authors.affiliation: Graduate Program in Clinical Hematology Sciences, Department of Clinical Microscopy, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand.
authors.forename: C
authors.initials: C
authors.lastname: Parnsamut
authors.affiliation: Center for Research and Development in Molecular Hematology Sciences, Department of Clinical Microscopy, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand Sirikalaya.J@chula.ac.th.
authors.forename: S
authors.initials: S
authors.lastname: Brimson
mesh_terms: D015153:Blotting, Western; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D006046:Gold / Q000008:administration & dosage* / Q000737:chemistry; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis*; D015850:Interleukin-6 / Q000096:biosynthesis*; D019169:Jurkat Cells; D007938:Leukemia / Q000378:metabolism / Q000473:pathology; D008070:Lipopolysaccharides / Q000494:pharmacology; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D053768:Metal Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D010835:Phytohemagglutinins / Q000494:pharmacology; D012834:Silver / Q000008:administration & dosage* / Q000737:chemistry; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*; D020298:U937 Cells
chemical_list: D007376:Interleukin-2; D015850:Interleukin-6; D008070:Lipopolysaccharides; D010835:Phytohemagglutinins; D014409:Tumor Necrosis Factor-alpha; D012834:Silver; D006046:Gold; D020928:Mitogen-Activated Protein Kinases; D013755:Tetradecanoylphorbol Acetate