title: MicroRNA-25 promotes gastric cancer proliferation, invasion, and migration by directly targeting F-box and WD-40 Domain Protein 7, FBXW7.
abstract: Increasing evidence shows that abnormal microRNA (miRNA) expression is involved in tumorigenesis. MiR-25 was previously reported to act as tumor suppressor or oncogene in diverse cancers. However, their expression, function, and mechanism in gastric cancer (GC) are not well known. Here, we show that miR-25 was overexpressed in primary tumor tissues of GC patients and was significantly correlated with a more aggressive phenotype of GC in patients. MiR-25 inhibition significantly decreased the proliferation, invasion, and migration of GC cells in vitro. Furthermore, miR-25 repressed F-box and WD-40 domain protein 7 (FBXW7) expression by directly binding to 3-untranslated region (UTR) of FBXW7, and the inverse correlation was observed between the expressions of miR-25 and FBXW7 mRNA in primary GC tissues. Moreover, the restoration of FBXW7 led to suppressed proliferation, invasion, and migration of GC cells. In vivo, miR-25 promotes tumor growth of GC. Taken together, miR-25 promotes GC progression by directly downregulating FBXW7 expression and may be employed as a novel prognostic marker and therapeutic target of GC.
authors.affiliation: Health checkup Center, Beidaihe Sanatorium of Beijing Military Area Command, Chinese PLA, Qinhuangdao, 066000, People's Republic of China.
authors.forename: Junhua
authors.initials: J
authors.lastname: Gong
authors.affiliation: Health checkup Center, Beidaihe Sanatorium of Beijing Military Area Command, Chinese PLA, Qinhuangdao, 066000, People's Republic of China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Cui
authors.affiliation: Health checkup Center, Beidaihe Sanatorium of Beijing Military Area Command, Chinese PLA, Qinhuangdao, 066000, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Health checkup Center, Beidaihe Sanatorium of Beijing Military Area Command, Chinese PLA, Qinhuangdao, 066000, People's Republic of China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ma
authors.affiliation: Health checkup Center, Beidaihe Sanatorium of Beijing Military Area Command, Chinese PLA, Qinhuangdao, 066000, People's Republic of China.
authors.forename: Qiufang
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Health checkup Center, Beidaihe Sanatorium of Beijing Military Area Command, Chinese PLA, Qinhuangdao, 066000, People's Republic of China.
authors.forename: Yinhe
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Health checkup Center, Beidaihe Sanatorium of Beijing Military Area Command, Chinese PLA, Qinhuangdao, 066000, People's Republic of China. sunhaodoctor@163.com.
authors.forename: Hao
authors.initials: H
authors.lastname: Sun
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D016022:Case-Control Studies; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002465:Cell Movement; D049109:Cell Proliferation; D044783:F-Box Proteins / Q000235:genetics / Q000378:metabolism*; D000073979:F-Box-WD Repeat-Containing Protein 7; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D015996:Survival Rate; D014407:Tumor Cells, Cultured; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*; D023041:Xenograft Model Antitumor Assays
chemical_list: D020413:3' Untranslated Regions; D018797:Cell Cycle Proteins; D044783:F-Box Proteins; D000073979:F-Box-WD Repeat-Containing Protein 7; C513273:FBXW7 protein, human; C543351:MIRN25 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D044767:Ubiquitin-Protein Ligases
keywords: FBXW7; Gastric cancer; Invasion; MicroRNA-25; Migration; Proliferation