title: Comprehensive analysis of the T-cell receptor beta chain gene in rhesus monkey by high throughput sequencing.
abstract: Profiling immune repertoires by high throughput sequencing enhances our understanding of immune system complexity and immune-related diseases in humans. Previously, cloning and Sanger sequencing identified limited numbers of T cell receptor (TCR) nucleotide sequences in rhesus monkeys, thus their full immune repertoire is unknown. We applied multiplex PCR and Illumina high throughput sequencing to study the TCRβ of rhesus monkeys. We identified 1.26 million TCRβ sequences corresponding to 643,570 unique TCRβ sequences and 270,557 unique complementarity-determining region 3 (CDR3) gene sequences. Precise measurements of CDR3 length distribution, CDR3 amino acid distribution, length distribution of N nucleotide of junctional region, and TCRV and TCRJ gene usage preferences were performed. A comprehensive profile of rhesus monkey immune repertoire might aid human infectious disease studies using rhesus monkeys.
authors.affiliation: Department of Biology, South University of Science and Technology of China, Shenzhen 518055, China.
authors.forename: Zhoufang
authors.initials: Z
authors.lastname: Li
authors.affiliation: State Key Laboratory of Respiratory Disease, Center for Infection and Immunity, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
authors.forename: Guangjie
authors.identifier: 0000000265669210
authors.initials: G
authors.lastname: Liu
authors.affiliation: Department of Biology, South University of Science and Technology of China, Shenzhen 518055, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Tong
authors.affiliation: Department of Biology, South University of Science and Technology of China, Shenzhen 518055, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Infectious Diseases and Hepatology Unit, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Respiratory Disease, Center for Infection and Immunity, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
authors.forename: Li
authors.initials: L
authors.lastname: Qin
authors.affiliation: Department of Infectious Diseases and Hepatology Unit, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Zhanhui
authors.initials: Z
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Respiratory Disease, Center for Infection and Immunity, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Biology, South University of Science and Technology of China, Shenzhen 518055, China.
authors.forename: Jiankui
authors.initials: J
authors.lastname: He
mesh_terms: D000818:Animals; D022801:Complementarity Determining Regions / Q000235:genetics*; D008253:Macaca mulatta; D060885:Multiplex Polymerase Chain Reaction; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D022801:Complementarity Determining Regions; D016693:Receptors, Antigen, T-Cell, alpha-beta