title: Treatment of Dutch rat models of glioma using EphrinA1-PE38/GM-CSF chitosan nanoparticles by in situ activation of dendritic cells.
abstract: Although dendritic cells (DCs) used in DC-based immunotherapy are loaded with tumor-associated antigens, the antitumor immune response is effectively stimulated in cytotoxic specific T lymphocytes (CTLs), thereby achieving targeted killing of tumor cells without harming surrounding normal cells. This makes it a highly promising new form of therapy. In this study, we successfully created chitosan-coated EphrinA1-PE38/GM-CSF nanoparticles and transplanted them into tumor-bearing rats, resulting in the effective killing of glioma tissue and a prolonged life span. Further immunohistochemistry and flow cytometry studies revealed that the treatment was effective in increasing the number of dendritic cell activations under an immunomodulatory response. These results suggest that chitosan-coated EphrinA1-PE38/GM-CSF nanoparticles may be effective in inducing in situ activation of DCs in glioma-bearing rats, thereby generating DC vaccines in vivo.
authors.affiliation: Department of Neurosurgery, the People's Hosptial of Zhengzhou University, 7 Weiwu Road, Zhengzhou City, Henan Province, 450003, People's Republic of China.
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, the People's Hosptial of Zhengzhou University, 7 Weiwu Road, Zhengzhou City, Henan Province, 450003, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, the People's Hosptial of Zhengzhou University, 7 Weiwu Road, Zhengzhou City, Henan Province, 450003, People's Republic of China.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Department of Neurosurgery, the People's Hosptial of Zhengzhou University, 7 Weiwu Road, Zhengzhou City, Henan Province, 450003, People's Republic of China.
authors.forename: Xiwen
authors.initials: X
authors.lastname: Shi
authors.affiliation: Department of Neurosurgery, the People's Hosptial of Zhengzhou University, 7 Weiwu Road, Zhengzhou City, Henan Province, 450003, People's Republic of China. 13733185588@163.com.
authors.forename: Jiadong
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Central Research Lab, the People's Hosptial of Zhengzhou University, 7 Weiwu Road, Zhengzhou City, Henan Province, 450003, People's Republic of China. 13598888356@163.com.
authors.forename: Shuangyin
authors.initials: S
authors.lastname: Han
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000276:immunology / Q000473:pathology / Q000628:therapy; D048271:Chitosan / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D036382:Ephrin-A1 / Q000276:immunology*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D005910:Glioma / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000276:immunology*; D007124:Immunoenzyme Techniques; D007167:Immunotherapy; D053758:Nanoparticles / Q000008:administration & dosage*; D051381:Rats; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014407:Tumor Cells, Cultured
chemical_list: D036382:Ephrin-A1; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D048271:Chitosan
keywords: Chitosan nanoparticles; Dendritic cells; EphrinA1; GM-CSF; Glioma