title: Enolase-1 is a therapeutic target in endometrial carcinoma.
abstract: ENO1 plays a paradoxical role in driving the pathogenesis of tumors. However, the clinical significance of ENO1 expression remains unclear and its function and modulatory mechanisms have never been reported in endometrial carcinoma (EC). In this study, ENO1 silencing significantly reduced cell glycolysis, proliferation, migration, and invasion in vitro, as well as tumorigenesis and metastasis in vivo by modulating p85 suppression. This in turn mediated inactivation of PI3K/AKT signaling and its downstream signals including glycolysis, cell cycle progression, and epithelial-mesenchymal transition (EMT)-associated genes. These effects on glycolysis and cell growth were not observed after ENO1 suppression in normal human endometrial epithelial cells (HEEC). Knocking down ENO1 could significantly enhance the sensitivity of EC cells to cisplatin (DDP) and markedly inhibited the growth of EC xenografts in vivo. In clinical samples, EC tissues exhibited higher expression levels of ENO1 mRNA and protein compared with normal endometrium tissues. Patients with higher ENO1 expression had a markedly shorter overall survival than patients with low ENO1 expression. We conclude that ENO1 favors carcinogenesis, representing a potential target for gene-based therapy.
authors.affiliation: Cancer Center, Traditional Chinese Medicine-Integrated Hospital of Southern Medical University, Guangzhou, Guangdong, China.|Cancer Research Institute, Southern Medical University, Guangzhou, China.
authors.forename: Mengyang
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Cancer Center, Traditional Chinese Medicine-Integrated Hospital of Southern Medical University, Guangzhou, Guangdong, China.|Cancer Research Institute, Southern Medical University, Guangzhou, China.
authors.forename: Weiyi
authors.initials: W
authors.lastname: Fang
authors.affiliation: Cancer Research Institute, Southern Medical University, Guangzhou, China.|Department of Obstetrics and Gynecology of Nanfang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Southern Medical University, Guangzhou, China.
authors.forename: Suiqun
authors.initials: S
authors.lastname: Guo
authors.affiliation: Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Southern Medical University, Guangzhou, China.
authors.forename: Luyun
authors.initials: L
authors.lastname: Shu
authors.affiliation: Cancer Research Institute, Southern Medical University, Guangzhou, China.|Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Southern Medical University, Guangzhou, China.
authors.forename: Lijing
authors.initials: L
authors.lastname: Wang
authors.affiliation: Cancer Research Institute, Southern Medical University, Guangzhou, China.
authors.forename: YiYu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Cancer Research Institute, Southern Medical University, Guangzhou, China.
authors.forename: Qiaofen
authors.initials: Q
authors.lastname: Fu
authors.affiliation: Cancer Research Institute, Southern Medical University, Guangzhou, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Cancer Research Institute, Southern Medical University, Guangzhou, China.
authors.forename: Shengni
authors.initials: S
authors.lastname: Hua
authors.affiliation: Cancer Research Institute, Southern Medical University, Guangzhou, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Cancer Research Institute, Southern Medical University, Guangzhou, China.
authors.forename: Yiyi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Cancer Research Institute, Southern Medical University, Guangzhou, China.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Deng
authors.affiliation: Cancer Center, Traditional Chinese Medicine-Integrated Hospital of Southern Medical University, Guangzhou, Guangdong, China.
authors.forename: Rongcheng
authors.initials: R
authors.lastname: Luo
authors.affiliation: Department of Obstetrics and Gynecology of Nanfang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Mei
authors.affiliation: Cancer Research Institute, Southern Medical University, Guangzhou, China.|Department of Pathology, Third Affiliated Hospital of Guangzhou Medical College, Guangzhou, China.
authors.forename: Qinping
authors.initials: Q
authors.lastname: Jiang
authors.affiliation: Cancer Research Institute, Southern Medical University, Guangzhou, China.|Department of Pathology of Basic School, Medical University of Guangzhou, Guangzhou, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002945:Cisplatin / Q000494:pharmacology; D058543:Class Ia Phosphatidylinositol 3-Kinase / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics*; D016889:Endometrial Neoplasms / Q000401:mortality / Q000473:pathology*; D004717:Endometrium / Q000473:pathology; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006019:Glycolysis / Q000235:genetics; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness / Q000235:genetics; D009368:Neoplasm Transplantation; D010751:Phosphopyruvate Hydratase / Q000235:genetics*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D034622:RNA Interference; D012333:RNA, Messenger / Q000096:biosynthesis; D034741:RNA, Small Interfering; D015398:Signal Transduction / Q000235:genetics; D014183:Transplantation, Heterologous; D025521:Tumor Suppressor Proteins / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D025521:Tumor Suppressor Proteins; D058543:Class Ia Phosphatidylinositol 3-Kinase; D051057:Proto-Oncogene Proteins c-akt; C095157:ENO1 protein, human; D010751:Phosphopyruvate Hydratase; D002945:Cisplatin
keywords: EMT; ENO1; cell growth; endometrial carcinoma; glycolysis