title: Effect of sealing infected dentin with glass ionomer cement on the abundance and localization of MMP-2, MMP-8, and MMP-9 in young permanent molars in vivo.
abstract: BACKGROUND
  The study of MMPs' behavior in carious lesions contributes to the understanding of the mechanisms involved in dentin reorganization after restoration.
  
  
  OBJECTIVE
  To compare the abundance and localization of MMPs 2, 8, and 9 in infected dentin before and after restoration.
  
  
  METHODS
  The sample consisted of 23 young permanent molars with active deep carious lesions. Infected carious dentin samples were collected from the same tooth at baseline and 60 days after cavity lining with GIC and composite resin restoration and processed for immunohistochemistry assays. After digital images were obtained, two calibrated operators analyzed the samples according to the immunostaining intensity and the MMPs' localization. Chi-square test was used for statistical analysis.
  
  
  RESULTS
  The intensity of immunostaining for MMP-8 was reduced after 60 days (P = 0.02), and no difference was observed for MMP-2 (P = 0.32) and MMP-9 (P = 0.14). The MMPs' distribution was generalized in the intertubular dentin and absent or located in the intratubular dentin, regardless of the period.
  
  
  CONCLUSIONS
  The sealing of infected carious dentin in young permanent molars reduced the expression of MMP-8, which is consistent with the initial remodeling process of the dentin matrix.
authors.affiliation: Department of Dentistry, School of Dentistry, Ponta Grossa State University, Ponta Grossa, Brazil.
authors.forename: Eunice
authors.initials: E
authors.lastname: Kuhn
authors.affiliation: Department of Dentistry, School of Dentistry, Ponta Grossa State University, Ponta Grossa, Brazil.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Reis
authors.affiliation: Department of Dentistry, School of Dentistry, Ponta Grossa State University, Ponta Grossa, Brazil.
authors.forename: Eduardo Bauml
authors.initials: EB
authors.lastname: Campagnoli
authors.affiliation: Department of Dentistry, School of Dentistry, Ponta Grossa State University, Ponta Grossa, Brazil.
authors.forename: Ana Cláudia Rodrigues
authors.initials: AC
authors.lastname: Chibinski
authors.affiliation: Departament of Dentistry, Bandeirante Anhanguera University, São Paulo, Brazil.
authors.forename: Marcela Rocha de Oliveira
authors.initials: MR
authors.lastname: Carrilho
authors.affiliation: Department of Dentistry, School of Dentistry, Ponta Grossa State University, Ponta Grossa, Brazil.
authors.forename: Denise Stadler
authors.initials: DS
authors.lastname: Wambier
mesh_terms: D000293:Adolescent; D001938:Brazil; D002648:Child; D003188:Composite Resins; D003731:Dental Caries / Q000378:metabolism* / Q000473:pathology* / Q000628:therapy*; D003804:Dentin / Q000473:pathology*; D019229:Dentition, Permanent; D005260:Female; D005899:Glass Ionomer Cements; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020784:Matrix Metalloproteinase 8 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D008963:Molar
chemical_list: D003188:Composite Resins; D005899:Glass Ionomer Cements; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; D020784:Matrix Metalloproteinase 8; D020780:Matrix Metalloproteinase 9