title: Lentivirus mediated over expression of CGRP inhibited oxidative stress in Schwann cell line.
abstract: CGRP is reported to be implicated in the process of diabetes and neuronal disease. However, the role and underlying mechanism of CGRP involved in diabetic neuropathy is unknown. Schwann cells play a central role in diabetic neuropathy, therefore the protective effect of CGRP on Schwann cells exposed to high glucose is determined. In the present study, full-length CGRP cDNA was isolated and then transferred to gateway adapted lentivirus expression vector by LR recombination reaction. Afterwards, the CGRP bearing recombinant virus was prepared in 293 FT cells and used to infect Schwann cells. The viability and superoxide anions of Schwann cells were evaluated following stimulation with high glucose, and levels of SOD, MDA and NOX1 were assessed. The results suggested that CGRP expression was up-regulated following lentivirus transfection. Lenti-CGRP increased cell viability in high glucose, but the effect was transient. Further lenti-CGRP protected against oxidative stress in Schwann cells triggered by high glucose and lenti-CGRP was effective in increasing SOD and decreasing MDA level. Meanwhile, the increased level of NOX1 caused by high glucose was reversed by lenti-CGRP overexpression. We therefore, suggest that lenti-CGRP may play a role in inhibiting oxidative stress in Schwann cell lines following hyperglycemic stimulation.
authors.affiliation: Beijing University of Chinese Medicine, Beijing, China. Electronic address: nayattmm@vip.sina.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Beijing University of Chinese Medicine, Beijing, China. Electronic address: haogaimei@163.com.
authors.forename: Gai-Mei
authors.initials: GM
authors.lastname: Hao
authors.affiliation: Beijing University of Chinese Medicine, Beijing, China. Electronic address: 1296689434@qq.com.
authors.forename: Jie
authors.initials: J
authors.lastname: He
authors.affiliation: Beijing University of Chinese Medicine, Beijing, China. Electronic address: lvtiantian471398@163.com.
authors.forename: Tian-Tian
authors.initials: TT
authors.lastname: Lv
authors.affiliation: Beijing University of Chinese Medicine, Beijing, China. Electronic address: 2357565806@qq.com.
authors.forename: Hong-Liang
authors.initials: HL
authors.lastname: Wang
authors.affiliation: Beijing University of Chinese Medicine, Beijing, China. Electronic address: 20030724mao@sina.cn.
authors.forename: Ying-Qiu
authors.initials: YQ
authors.lastname: Mao
authors.affiliation: Beijing University of Chinese Medicine, Beijing, China. Electronic address: wangyrong@yahoo.com.cn.
authors.forename: Xue
authors.initials: X
authors.lastname: Wang
authors.affiliation: Beijing University of Chinese Medicine, Beijing, China. Electronic address: wangwei26960@126.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Beijing University of Chinese Medicine, Beijing, China. Electronic address: hanjing8585@163.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Han
mesh_terms: D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002470:Cell Survival; D005822:Genetic Vectors; D005947:Glucose / Q000494:pharmacology; D016086:Lentivirus / Q000235:genetics*; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D000074624:NADPH Oxidase 1; D018384:Oxidative Stress; D051381:Rats; D012583:Schwann Cells / Q000166:cytology / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D013481:Superoxides; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D009247:NADH, NADPH Oxidoreductases; D000074624:NADPH Oxidase 1; C000614711:NOX1 protein, mouse; C000614712:NOX1 protein, rat; D005947:Glucose; D015740:Calcitonin Gene-Related Peptide
keywords: CGRP; Oxidative stress; Schwann cell