title: Polymer Coatings in 3D-Printed Fluidic Device Channels for Improved Cellular Adherence Prior to Electrical Lysis.
abstract: This paper describes the design and fabrication of a polyjet-based three-dimensional (3D)-printed fluidic device where poly(dimethylsiloxane) (PDMS) or polystyrene (PS) were used to coat the sides of a fluidic channel within the device to promote adhesion of an immobilized cell layer. The device was designed using computer-aided design software and converted into an .STL file prior to printing. The rigid, transparent material used in the printing process provides an optically transparent path to visualize endothelial cell adherence and supports integration of removable electrodes for electrical cell lysis in a specified portion of the channel (1 mm width × 0.8 mm height × 2 mm length). Through manipulation of channel geometry, a low-voltage power source (500 V max) was used to selectively lyse adhered endothelial cells in a tapered region of the channel. Cell viability was maintained on the device over a 5 day period (98% viable), though cell coverage decreased after day 4 with static media delivery. Optimal lysis potentials were obtained for the two fabricated device geometries, and selective cell clearance was achieved with cell lysis efficiencies of 94 and 96%. The bottleneck of unknown surface properties from proprietary resin use in fabricating 3D-printed materials is overcome through techniques to incorporate PDMS and PS.
authors.affiliation: Department of Chemistry, Michigan State University, East Lansing, Michigan 48823, United States.
authors.forename: Bethany C
authors.initials: BC
authors.lastname: Gross
authors.affiliation: Department of Chemistry, Michigan State University, East Lansing, Michigan 48823, United States.
authors.forename: Kari B
authors.initials: KB
authors.lastname: Anderson
authors.affiliation: Department of Chemistry, Michigan State University, East Lansing, Michigan 48823, United States.
authors.forename: Jayda E
authors.initials: JE
authors.lastname: Meisel
authors.affiliation: Department of Chemistry, Michigan State University, East Lansing, Michigan 48823, United States.
authors.forename: Megan I
authors.initials: MI
authors.lastname: McNitt
authors.affiliation: Department of Chemistry, Michigan State University, East Lansing, Michigan 48823, United States.
authors.forename: Dana M
authors.initials: DM
authors.lastname: Spence
mesh_terms: D002448:Cell Adhesion; D002457:Cell Extracts / Q000302:isolation & purification; D002470:Cell Survival; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D004566:Electrodes; D042783:Endothelial Cells / Q000166:cytology; D004867:Equipment Design; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation; D011137:Polystyrenes / Q000737:chemistry*; D066330:Printing, Three-Dimensional / Q000295:instrumentation
chemical_list: D002457:Cell Extracts; D020099:Coated Materials, Biocompatible; D004129:Dimethylpolysiloxanes; D011137:Polystyrenes; C013830:baysilon