title: Method for Preparation and Electroporation of S. aureus and S. epidermidis.
abstract: For bacterial species that are not known to be naturally competent, such as Staphylococcus aureus and Staphylococcus epidermidis, electroporation is an efficient method for introducing genetic material into the cell. The technique utilizes electrical pulses to transiently permeabilize bacterial cell membranes, which allows for the passage of plasmid DNA across the membranes. Here, we describe methods for preparing electrocompetent S. aureus and S. epidermidis cells and outline a procedure for electroporation of the prepared competent cells.
authors.affiliation: Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, 125 Mason Farm Rd, Marsico Hall 6209, Chapel Hill, NC, 27599, USA.
authors.forename: Melinda R
authors.initials: MR
authors.lastname: Grosser
authors.affiliation: Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, 125 Mason Farm Rd, Marsico Hall 6209, Chapel Hill, NC, 27599, USA. anthony_richardson@med.unc.edu.
authors.forename: Anthony R
authors.initials: AR
authors.lastname: Richardson
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics; D018274:Electroporation / Q000379:methods*; D006801:Humans; D010957:Plasmids; D013203:Staphylococcal Infections / Q000235:genetics / Q000382:microbiology / Q000473:pathology; D013211:Staphylococcus aureus / Q000235:genetics* / Q000472:pathogenicity; D013212:Staphylococcus epidermidis / Q000235:genetics* / Q000472:pathogenicity; D014169:Transformation, Bacterial / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial
keywords: Competent cells; Electrocompetent; Electroporation; Transformation