title: Gender- and region-dependent changes of redox biomarkers in the brain of successfully aging LOU/C rats.
abstract: The LOU/C (LOU) rat is an obesity resistant strain with higher longevity and healthspan than common rats. The management of oxidative stress being important to successful aging, we characterized this process in the aging LOU rat. Male/female LOU rats were euthanized at 4, 20, and 29 months. Macrodissected hippocampus, striatum, parietal cortex, cerebellum were assayed for tissue concentrations of glutathione (GSH), gamma-glutamyl-cysteine-synthetase (γ-GCS), total thiols, protein carbonyls, mRNAs of clusterin and the known protective enzymes thioredoxine-1 (TRX-1), glutaredoxine-1 (GLRX-1), superoxide dismutase-1 (SOD-1). Brain levels of GSH, γ-GCS, total thiols remained constant with age, except for GSH and γ-GCS which decreases in females. Clusterin, TRX-1, GLRX-1, SOD-1 mRNA levels were maintained or increased in the hippocampus with age. Age-dependency of the markers differed between sexes, with SOD-1 and TRX-1 decreases out of hippocampus in females. Since antioxidants were reported to decrease with age in the brain of Wistar rats, maintenance of GSH levels and of protective enzymes mRNA levels in the LOU rat brain could contribute to the preservation of cognitive functions in old age. Altogether, the successful aging of LOU rats may, at least in part, involve the conservation of functional antioxidant mechanisms in the brain, supporting the oxidative stress theory of aging.
authors.affiliation: Laboratory of Neuroendocrinology of Aging, Centre Hospitalier de l'Université de Montréal Research Center (CRCHUM), 900 St-Denis street, Viger Tower, Rm R05.436B-02, Montreal, QC H2X 0A9, Canada; Physiology of Reproduction and Behaviour Unit (PRC), Centre INRA of Tours, University François Rabelais of Tours, F-37380 Nouzilly, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Moyse
authors.affiliation: Institut Armand-Frappier, INRS, 531 Blvd des Prairies, Laval, QC H7 V 1B7, Canada.
authors.forename: Madeleine
authors.initials: M
authors.lastname: Arseneault
authors.affiliation: Laboratory of Neuroendocrinology of Aging, Centre Hospitalier de l'Université de Montréal Research Center (CRCHUM), 900 St-Denis street, Viger Tower, Rm R05.436B-02, Montreal, QC H2X 0A9, Canada; Department of Medicine, University of Montreal, Montreal, QC H3C 3J7, Canada.
authors.forename: Pierrette
authors.initials: P
authors.lastname: Gaudreau
authors.affiliation: Sacré-Cœur Hospital Research Center, Montreal, QC H4J 1C5, Canada; Department of Nutrition, University of Montreal, Montreal, QC H3C 3J7, Canada.
authors.forename: Guylaine
authors.initials: G
authors.lastname: Ferland
authors.affiliation: Institut Armand-Frappier, INRS, 531 Blvd des Prairies, Laval, QC H7 V 1B7, Canada. Electronic address: Charles.Ramassamy@iaf.inrs.ca.
authors.forename: Charles
authors.initials: C
authors.lastname: Ramassamy
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D002531:Cerebellum / Q000378:metabolism; D051152:Clusterin / Q000378:metabolism; D005260:Female; D005721:Glutamate-Cysteine Ligase / Q000378:metabolism; D054477:Glutaredoxins / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism; D008297:Male; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012737:Sex Factors; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D051152:Clusterin; C516005:GLRX protein, human; D054477:Glutaredoxins; D012333:RNA, Messenger; D013438:Sulfhydryl Compounds; D013482:Superoxide Dismutase; D005721:Glutamate-Cysteine Ligase; D005978:Glutathione; D010100:Oxygen
keywords: Clusterin; Glutaredoxine-1; Glutathione; Protein carbonyl; Superoxide dismutase-1; Thioredoxine-1