title: Genetic tools for manipulating Acinetobacter baumannii genome: an overview.
abstract: Acinetobacter baumannii is an emerging nosocomial pathogen involved in a variety of infections ranging from minor soft-tissue infections to more severe infections such as ventilator-associated pneumonia and bacteraemia. A. baumannii has become resistant to most of the commonly used antibiotics and multidrug-resistant isolates are becoming a severe problem in the healthcare setting. In the past few years, whole-genome sequences of >200 A. baumannii isolates have been generated. Several methods and molecular tools have been used for genetic manipulation of various Acinetobacter spp. Here, we review recent developments of various genetic tools used for modification of the A. baumannii genome, including various ways to inactivate gene function, chromosomal integration and transposon mutagenesis.
authors.affiliation: Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, 3901 Rainbow Boulevard, Kansas City, KS 66160, USA.
authors.forename: Indranil
authors.initials: I
authors.lastname: Biswas
mesh_terms: D040981:Acinetobacter baumannii / Q000235:genetics*; D003428:Cross Infection / Q000382:microbiology; D004251:DNA Transposable Elements / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D018274:Electroporation / Q000379:methods; D018014:Gene Transfer Techniques; D016680:Genome, Bacterial / Q000235:genetics*; D053717:Pneumonia, Ventilator-Associated / Q000382:microbiology; D014170:Transformation, Genetic / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements