title: Expression of Interferon γ by Decidual Cells and Natural Killer Cells at the Human Implantation Site: Implications for Preeclampsia, Spontaneous Abortion, and Intrauterine Growth Restriction.
abstract: Human first-trimester decidual cells (FTDCs) chemoattract CXCR3-expressing circulating CD56(bright)CD16(-) natural killer (NK) cells, which increase uteroplacental blood flow by remodeling spiral arteries and arterioles. This recruitment reflects elevated FTDC expression of NK cell-recruiting induced protein 10 and interferon (IFN)-inducible T-cell-α chemoattractant produced in response to the synergistic effects of tumor necrosis factor α (TNF-α) and IFN-γ stimulation. Decidual macrophages express TNF-α, whereas the cellular origin of IFN-γ is unclear. Therefore, this study aims to identify the cell source(s) of IFN-γ in human first trimester decidua. Immunostaining of decidual sections revealed that both FTDCs and decidual NK (dNK) cells express IFN-γ. Although individual dNK cells express higher IFN-γ levels, the more numerous FTDCs account for greater proportion of total IFN-γ immunostaining. Freshly isolated FTDCs express greater IFN-γ staining than dNK cells as measured by flow cytometry, whereas incubation of dNK cells with documented NK cell activators significantly increases IFN-γ above FTDC levels. Confluent FTDCs intrinsically produce, but paradoxically respond to, exogenous IFN-γ.
authors.affiliation: Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei, Taiwan.
authors.forename: Chie-Pein
authors.initials: CP
authors.lastname: Chen
authors.affiliation: Department of Obstetrics and Gynecology, The Ohio State University College of Medicine, Columbus, OH, USA.
authors.forename: Longzhu
authors.initials: L
authors.lastname: Piao
authors.affiliation: Department of Hematology, The Ohio State University College of Medicine, Columbus, OH, USA.
authors.forename: Xilin
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Hematology, The Ohio State University College of Medicine, Columbus, OH, USA.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Obstetrics and Gynecology, Beth Israel Medical Center, New York, NY, USA.
authors.forename: Rachel
authors.initials: R
authors.lastname: Masch
authors.affiliation: Department of Obstetrics and Gynecology, Morsani College of Medicine, University of South Florida, Tampa, FL, USA.
authors.forename: Frederick
authors.initials: F
authors.lastname: Schatz
authors.affiliation: Department of Obstetrics and Gynecology, Morsani College of Medicine, University of South Florida, Tampa, FL, USA cjlockwood@health.usf.edu.
authors.forename: Charles J
authors.initials: CJ
authors.lastname: Lockwood
authors.affiliation: Department of Obstetrics and Gynecology, The Ohio State University College of Medicine, Columbus, OH, USA joseph.huang@osumc.edu.
authors.forename: S Joseph
authors.initials: SJ
authors.lastname: Huang
mesh_terms: D000022:Abortion, Spontaneous / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D002478:Cells, Cultured; D003656:Decidua / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D010064:Embryo Implantation; D005260:Female; D005317:Fetal Growth Retardation / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D053583:Interleukin-1beta / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: C573415:IFNG protein, human; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma
keywords: NK cells; decidual cells; interferon γ