title: Isolation of the Thogoto virus from a Haemaphysalis longicornis in Kyoto City, Japan.
abstract: Ticks transmit viruses responsible for severe emerging and re-emerging infectious diseases, some of which have a significant impact on public health. In Japan, little is known about the distribution of tick-borne viruses. In this study, we collected and tested ticks to investigate the distribution of tick-borne arboviruses in Kyoto, Japan, and isolated the first Thogoto virus (THOV) to our knowledge from Haemaphysalis longicornis in far-eastern Asia. The Japanese isolate was genetically distinct from a cluster of other isolates from Africa, Europe and the Middle East. Various cell lines derived from mammals and ticks were susceptible to the isolate, but it was not pathogenic in mice. These results advance understanding of the distribution and ecology of THOV.
authors.affiliation: Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University, Kita-ku Kita-18 Nishi-9, Sapporo, Hokkaido 060-0818, Japan.|Department of Animal Medical Sciences, Faculty of Life Sciences, Kyoto Sangyo University, Motoyama, Kamigamo, Kita-ku, Kyoto City 603-8555, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Yoshii
authors.affiliation: Laboratory of Environmental Hygiene, Department of Animal Medical Sciences, Faculty of Life Sciences, Kyoto Sangyo University, Motoyama, Kamigamo, Kita-ku, Kyoto City 603-8555, Japan.
authors.forename: Natsumi
authors.initials: N
authors.lastname: Okamoto
authors.affiliation: Unit of Risk Analysis and Management, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Hokkaido 001-0020, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Nakao
authors.affiliation: Laboratory of Environmental Hygiene, Department of Animal Medical Sciences, Faculty of Life Sciences, Kyoto Sangyo University, Motoyama, Kamigamo, Kita-ku, Kyoto City 603-8555, Japan.
authors.forename: Robert
authors.initials: R
authors.lastname: Klaus Hofstetter
authors.affiliation: Laboratory of Environmental Hygiene, Department of Animal Medical Sciences, Faculty of Life Sciences, Kyoto Sangyo University, Motoyama, Kamigamo, Kita-ku, Kyoto City 603-8555, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Yabu
authors.affiliation: Laboratory of Environmental Hygiene, Department of Animal Medical Sciences, Faculty of Life Sciences, Kyoto Sangyo University, Motoyama, Kamigamo, Kita-ku, Kyoto City 603-8555, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Masumoto
authors.affiliation: Laboratory of Bacteriology, Department of Animal Medical Sciences, Faculty of Life Science, Kyoto Sangyo University, Motoyama, Kamigamo, Kita-ku, Kyoto City 603-8555, Japan.
authors.forename: Azusa
authors.initials: A
authors.lastname: Someya
authors.affiliation: Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University, Kita-ku Kita-18 Nishi-9, Sapporo, Hokkaido 060-0818, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kariwa
authors.affiliation: Laboratory of Environmental Hygiene, Department of Animal Medical Sciences, Faculty of Life Sciences, Kyoto Sangyo University, Motoyama, Kamigamo, Kita-ku, Kyoto City 603-8555, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Maeda
mesh_terms: D000818:Animals; D001096:Arachnid Vectors / Q000821:virology*; D005260:Female; D006801:Humans; D026863:Ixodidae / Q000821:virology*; D007564:Japan; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010802:Phylogeny; D018117:Thogotovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D017282:Tick-Borne Diseases / Q000635:transmission / Q000821:virology*