title: Proteomics methods for discovering viral-host interactions.
abstract: The functions of many viral proteins involve direct interactions with specific host proteins. Therefore considerable insight into the functions of a viral protein and its mechanisms of action can come from applying proteomics approaches to viral proteins in order to identify their cellular binding partners. In this chapter we describe proteomics approaches that have proven to be the most useful in identifying host interactions of viral proteins in human cells. Caveats and potential alternatives for each step are also discussed.
authors.affiliation: Department of Molecular Genetics, University of Toronto, 1 Kings College Circle, Toronto M5S 1A8, Canada.
authors.forename: Anna A
authors.initials: AA
authors.lastname: Georges
authors.affiliation: Department of Molecular Genetics, University of Toronto, 1 Kings College Circle, Toronto M5S 1A8, Canada. Electronic address: lori.frappier@utoronto.ca.
authors.forename: Lori
authors.initials: L
authors.lastname: Frappier
mesh_terms: D054884:Host-Pathogen Interactions; D013058:Mass Spectrometry / Q000379:methods; D040901:Proteomics / Q000379:methods*; D014764:Viral Proteins / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D014764:Viral Proteins
keywords: Affinity purification; Mass spectrometry; Viral proteins