title: Adeno-associated virus-mediated BMP-7 and SOX9 in vitro co-transfection of human degenerative intervertebral disc cells.
abstract: Bone morphogenetic protein-7 (BMP-7) and SOX9 are important transcription factors in chondrogenesis. In this study, we examined the biological function of the adeno-associated virus (AAV)-mediated BMP-7 and SOX9 double gene in vitro co-transfection of nucleus pulposus cells of human degenerative intervertebral disc. Human intervertebral disc nucleus pulposus cells were cultured in vitro and subcultured for 5 generations. Using rAAV-BMP-7 and rAAV-SOX9 AAV2-type AAV viruses, the cells were divided into 4 groups: blank normal, BMP-7 transfection, SOX9 transfection, and BMP-7 and SOX9 co-transfection. After 48 h, expression of type II collagen and its mRNA in nucleus pulposus cells was determined. The expression of type II collagen in BMP-7 transfection, SOX9 transfection, and co-transfection groups was up-regulated to varying degrees compared to the blank control group. The type II collagen mRNA level expression in the co-transfection group was significantly higher than in other groups (P < 0.05). AAV-mediated BMP-7 and SOX9 in vitro co-transfection can promote the synthesis of type II collagen in nucleus pulposus cells in the human degenerative intervertebral disc. Double-gene therapy has a synergistic effect in the treatment of intervertebral disc degeneration.
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: X F
authors.initials: XF
authors.lastname: Ren
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Z Z
authors.initials: ZZ
authors.lastname: Diao
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Qingdao University, Qingdao, China yongmingxidoc@126.com.
authors.forename: Y M
authors.initials: YM
authors.lastname: Xi
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Z H
authors.initials: ZH
authors.lastname: Qi
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: S
authors.initials: S
authors.lastname: Ren
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Liu
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: D L
authors.initials: DL
authors.lastname: Yang
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: S L
authors.initials: SL
authors.lastname: Yuan
mesh_terms: D055419:Bone Morphogenetic Protein 7 / Q000096:biosynthesis / Q000235:genetics*; D002478:Cells, Cultured; D024043:Collagen Type II / Q000096:biosynthesis / Q000235:genetics; D000229:Dependovirus / Q000235:genetics*; D015870:Gene Expression; D015316:Genetic Therapy; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D007403:Intervertebral Disc / Q000473:pathology; D055959:Intervertebral Disc Degeneration / Q000378:metabolism / Q000628:therapy*; D055758:SOX9 Transcription Factor / Q000096:biosynthesis / Q000235:genetics*; D014162:Transfection
chemical_list: C525819:BMP7 protein, human; D055419:Bone Morphogenetic Protein 7; D024043:Collagen Type II; D055758:SOX9 Transcription Factor; C527932:SOX9 protein, human; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins