title: [Characteristics of karyotypes and gene mutations for elder acute myeloid leukemia].
abstract: OBJECTIVE
  To investigate the incidence of karyotypes and gene mutations for elder acute myeloid leukemia and to explore the relationship between each other.
  
  
  METHODS
  Clinical data and bone marrow samples of elder AML patients were collected. Karyotype and gene mutation (FLT3, NPM1, C-Kit, CEBPα, DNMT3A) test were performed, characteristics of karyotypes and gene mutations were analysed.
  
  
  RESULTS
  The incidence of better risk karyotype was 16.6%, in which the incidences of t(15;17), t(8;21) and inv (16)/t(16;16) were 3.90%, 10.73%, and 1.95% respectively; the incidence of intermediate risk karyotype was 72.2%, in which the incidence of normal karyotype was 57.86%; the incidence of poor risk karyotype was 11.20%, in which the incidence of of MLL/11q23, complex karyotype and monosomal karyotype were 1.95%, 6.34%, 5.85% respectively; the incidences of FLT3, NPM1, C-Kit, CEBPα, DNMT3A mutation were 12.57%, 22.06%, 2.16%, 14.71%, 15.71% respectively. Compared with patients older than 60 years, patients with age of 55-60 years were with less complex karyotype (1.09% vs 10.62%)(P=0.003) and monosomal karyotype (2.17% vs 8.85%)(P=0.032), and more t(8;21)(17.39% vs 5.31%)(P=0.008) and inv (16)/t(16;16)(4.35% vs 0.00%)(P=0.045).
  
  
  CONCLUSIONS
  For older AML patients, great difference in the distribution of karyotyes was found between the patients older than 60 years and patients with age of 55-60 years, while no such characteristics was found for gene mutations. Good elucidation of karyotypes and gene mutations are key for the treatment of older acute myeloid leukemia patients.
authors.affiliation: Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Unian Medical College, Tianjin 300020, China.
authors.forename: Xing-Li
authors.initials: XL
authors.lastname: Zhao
authors.affiliation: Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Unian Medical College, Tianjin 300020, China.
authors.forename: Kai-Qi
authors.initials: KQ
authors.lastname: Liu
authors.affiliation: Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Unian Medical College, Tianjin 300020, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Lin
authors.affiliation: Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Unian Medical College, Tianjin 300020, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wei
authors.affiliation: Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Unian Medical College, Tianjin 300020, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Unian Medical College, Tianjin 300020, China.
authors.forename: Chun-Lin
authors.initials: CL
authors.lastname: Zhou
authors.affiliation: Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Unian Medical College, Tianjin 300020, China.
authors.forename: Bing-Cheng
authors.initials: BC
authors.lastname: Liu
authors.affiliation: Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Unian Medical College, Tianjin 300020, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Unian Medical College, Tianjin 300020, China.
authors.forename: Cheng-Wen
authors.initials: CW
authors.lastname: Li
authors.affiliation: Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Unian Medical College, Tianjin 300020, China.
authors.forename: Qing-Hua
authors.initials: QH
authors.lastname: Li
authors.affiliation: Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Unian Medical College, Tianjin 300020, China.
authors.forename: Zeng
authors.initials: Z
authors.lastname: Cao
authors.affiliation: Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Unian Medical College, Tianjin 300020, China.
authors.forename: Ben-Fa
authors.initials: BF
authors.lastname: Gong
authors.affiliation: Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Unian Medical College, Tianjin 300020, China.
authors.forename: Yun-Tao
authors.initials: YT
authors.lastname: Liu
authors.affiliation: Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Unian Medical College, Tianjin 300020, China.
authors.forename: Xiao-Yuan
authors.initials: XY
authors.lastname: Gong
authors.affiliation: Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Unian Medical College, Tianjin 300020, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Unian Medical College, Tianjin 300020, China.
authors.forename: Run-Xia
authors.initials: RX
authors.lastname: Gu
authors.affiliation: Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Unian Medical College, Tianjin 300020, China.
authors.forename: Ying-Chang
authors.initials: YC
authors.lastname: Mi
authors.affiliation: Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Unian Medical College, Tianjin 300020, China. E-mail: wangjx@medmail.com.cn.
authors.forename: Jian-Xiang
authors.initials: JX
authors.lastname: Wang
mesh_terms: D006801:Humans; D015994:Incidence; D059785:Karyotype; D007621:Karyotyping; D008875:Middle Aged; D009154:Mutation; D000090243:Nucleophosmin; D019009:Proto-Oncogene Proteins c-kit
chemical_list: C000716967:NPM1 protein, human; D000090243:Nucleophosmin; D019009:Proto-Oncogene Proteins c-kit