title: Simple Method for Constructing RNA Triangle, Square, Pentagon by Tuning Interior RNA 3WJ Angle from 60° to 90° or 108°.
abstract: Precise shape control of architectures at the nanometer scale is an intriguing but extremely challenging facet. RNA has recently emerged as a unique material and thermostable building block for use in nanoparticle construction. Here, we describe a simple method from design to synthesis of RNA triangle, square, and pentagon by stretching RNA 3WJ native angle from 60° to 90° and 108°, using the three-way junction (3WJ) of the pRNA from bacteriophage phi29 dsDNA packaging motor. These methods for the construction of elegant polygons can be applied to other RNA building blocks including the utilization and application of RNA 4-way, 5-way, and other multi-way junctions.
authors.affiliation: Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lexington, KY, 40536, USA, kemil@bsu.edu.
authors.forename: Emil F
authors.initials: EF
authors.lastname: Khisamutdinov
authors.forename: My Nguyen Hoan
authors.initials: MN
authors.lastname: Bui
authors.forename: Daniel
authors.initials: D
authors.lastname: Jasinski
authors.forename: Zhengyi
authors.initials: Z
authors.lastname: Zhao
authors.forename: Zheng
authors.initials: Z
authors.lastname: Cui
authors.forename: Peixuan
authors.initials: P
authors.lastname: Guo
mesh_terms: D008958:Models, Molecular; D053758:Nanoparticles / Q000737:chemistry; D036103:Nanotechnology; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry* / Q000378:metabolism
chemical_list: D012313:RNA