title: Oxidative stress in bacteria (Pseudomonas putida) exposed to nanostructures of silicon carbide.
abstract: Silicon carbide (SiC) nanostructures produced by combustion synthesis can cause oxidative stress in the bacterium Pseudomonas putida. The results of this study showed that SiC nanostructures damaged the cell membrane, which can lead to oxidative stress in living cells and to the loss of cell viability. As a reference, micrometric SiC was also used, which did not exhibit toxicity toward cells. Oxidative stress was studied by analyzing the activity of peroxidases, and the expression of the glucose-6-phosphate dehydrogenase gene (zwf1) using real-time PCR and northern blot techniques. Damage to nucleic acid was studied by isolating and hydrolyzing plasmids with the formamidopyrimidine [fapy]-DNA glycosylase (also known as 8-oxoguanine DNA glycosylase) (Fpg), which is able to detect damaged DNA. The level of viable microbial cells was investigated by propidium iodide and acridine orange staining.
authors.affiliation: Faculty of Geology, University of Warsaw, Żwirki i Wigury 93, 02-089 Warsaw, Poland. Electronic address: a.borkowski@uw.edu.pl.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Borkowski
authors.affiliation: Faculty of Advanced Technologies and Chemistry, Military University of Technology, Kaliskiego 2, 00-908 Warsaw, Poland.
authors.forename: Mateusz
authors.initials: M
authors.lastname: Szala
authors.affiliation: Bionicum Ltd., Chełmska 21, Warsaw, Poland.
authors.forename: Paweł
authors.initials: P
authors.lastname: Kowalczyk
authors.affiliation: Department of General and Environmental Microbiology, Poznan University of Life Sciences, Szydłowska 50, 60-656 Poznan, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Cłapa
authors.affiliation: Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Dojazd 11, 60-632 Poznan, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Narożna
authors.affiliation: Department of General and Environmental Microbiology, Poznan University of Life Sciences, Szydłowska 50, 60-656 Poznan, Poland.
authors.forename: Marek
authors.initials: M
authors.lastname: Selwet
mesh_terms: D017554:Carbon Compounds, Inorganic / Q000633:toxicity*; D004249:DNA Damage; D045647:DNA Glycosylases; D043382:DNA-Formamidopyrimidine Glycosylase; D049329:Nanostructures; D018384:Oxidative Stress; D010957:Plasmids; D016958:Pseudomonas putida / Q000187:drug effects*; D017655:Silicon Compounds / Q000633:toxicity*
chemical_list: D017554:Carbon Compounds, Inorganic; D017655:Silicon Compounds; D045647:DNA Glycosylases; C479058:oxoguanine glycosylase 1, human; D043382:DNA-Formamidopyrimidine Glycosylase; C022088:silicon carbide
keywords: Nanofibers; Nanorods; Oxidative stress; Pseudomonas putida; Silicon carbide; Toxicity