title: MiRNA profile of osteosarcoma with CD117 and stro-1 expression: miR-1247 functions as an onco-miRNA by targeting MAP3K9.
abstract: microRNAs (miRNA) are regulators of gene expression, but little is known about miRNA expression profiles in stem cells of osteosarcoma (OS). C117 and Stro-1 are known stem cell markers of OS. In the study, CD117 and stro-1 positive (CD117(+)stro-1(+)) and CD117 and stro-1 negative (CD117(-)stro-1(-)) cells were isolated from MG63 cells CD117(+)stro-1(+) cells showed more metastatic ability and stem cell formation rate than CD117(-)stro-1(-) ones. To find the difference between CD117(+)stro-1(+) and CD117(-)stro-1(-) cells, the miRNA expression profile was examined using DNA microarray. MicroRNAs were differentially expressed in osteosarcoma cells with CD117(+)stro-1(+) and CD117(-)stro-1(-). The significant miRNAs included miR-15a, miR-302a, miR-423-5p, miR-1247, miR-1243 and others, which were confirmed by real time RT-PCR. The significant down-regulated miR-1247 was confirmed that was a potential tumor suppressor by targeting MAP3K9. Our results indicated that dysregulation of miRNAs is involved in osteosarcoma and miR-1247 plays an important role in progression of osteosarcoma.
authors.affiliation: Department of Medical Oncology, The First Affiliated Hospital of Bengbu Medical College Bengbu, Anhui 233000, China.
authors.forename: Fuyou
authors.initials: F
authors.lastname: Zhao
authors.affiliation: Department of Microbiology, Bengbu Medical College Bengbu, Anhui 233000, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Lv
authors.affiliation: Department of Pathology, The First Affiliated Hospital of Bengbu Medical College Bengbu, Anhui 233000, China.
authors.forename: Huaiyong
authors.initials: H
authors.lastname: Gan
authors.affiliation: Department of Medical Oncology, The First Affiliated Hospital of Bengbu Medical College Bengbu, Anhui 233000, China.
authors.forename: Yumei
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Medical Oncology, The First Affiliated Hospital of Bengbu Medical College Bengbu, Anhui 233000, China.
authors.forename: Ri
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Medical Oncology, The First Affiliated Hospital of Bengbu Medical College Bengbu, Anhui 233000, China.
authors.forename: Haoran
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Medical Oncology, The First Affiliated Hospital of Bengbu Medical College Bengbu, Anhui 233000, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Department of Respiratory Diseases, The First Affiliated Hospital of Bengbu Medical College Bengbu, Anhui 233000, China.
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000954:Antigens, Surface / Q000096:biosynthesis; D015153:Blotting, Western; D001859:Bone Neoplasms / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002469:Cell Separation; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D020930:MAP Kinase Kinase Kinases / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics*; D014411:Neoplastic Stem Cells / Q000473:pathology*; D020411:Oligonucleotide Array Sequence Analysis; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology; D019009:Proto-Oncogene Proteins c-kit / Q000096:biosynthesis; D060888:Real-Time Polymerase Chain Reaction; D059467:Transcriptome
chemical_list: D000954:Antigens, Surface; C000605548:MIRN1247 microRNA, human; D035683:MicroRNAs; C525591:STRO-1 antigen, human; D019009:Proto-Oncogene Proteins c-kit; D020930:MAP Kinase Kinase Kinases; C433243:MAP3K9 protein, human
keywords: MAP3K9; miR-1247; miRNA profile; osteosarcoma