title: Molybdenum Availability Is Key to Nitrate Removal in Contaminated Groundwater Environments.
abstract: The concentrations of molybdenum (Mo) and 25 other metals were measured in groundwater samples from 80 wells on the Oak Ridge Reservation (ORR) (Oak Ridge, TN), many of which are contaminated with nitrate, as well as uranium and various other metals. The concentrations of nitrate and uranium were in the ranges of 0.1 μM to 230 mM and <0.2 nM to 580 μM, respectively. Almost all metals examined had significantly greater median concentrations in a subset of wells that were highly contaminated with uranium (≥126 nM). They included cadmium, manganese, and cobalt, which were 1,300- to 2,700-fold higher. A notable exception, however, was Mo, which had a lower median concentration in the uranium-contaminated wells. This is significant, because Mo is essential in the dissimilatory nitrate reduction branch of the global nitrogen cycle. It is required at the catalytic site of nitrate reductase, the enzyme that reduces nitrate to nitrite. Moreover, more than 85% of the groundwater samples contained less than 10 nM Mo, whereas concentrations of 10 to 100 nM Mo were required for efficient growth by nitrate reduction for two Pseudomonas strains isolated from ORR wells and by a model denitrifier, Pseudomonas stutzeri RCH2. Higher concentrations of Mo tended to inhibit the growth of these strains due to the accumulation of toxic concentrations of nitrite, and this effect was exacerbated at high nitrate concentrations. The relevance of these results to a Mo-based nitrate removal strategy and the potential community-driving role that Mo plays in contaminated environments are discussed.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA.
authors.forename: Michael P
authors.initials: MP
authors.lastname: Thorgersen
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA.
authors.forename: W Andrew
authors.initials: WA
authors.lastname: Lancaster
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA.
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Vaccaro
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA.
authors.forename: Farris L
authors.initials: FL
authors.lastname: Poole
authors.affiliation: Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
authors.forename: Andrea M
authors.initials: AM
authors.lastname: Rocha
authors.affiliation: Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
authors.forename: Tonia
authors.initials: T
authors.lastname: Mehlhorn
authors.affiliation: Lawrence Berkeley National Laboratory, Berkeley, California, USA.
authors.forename: Angelica
authors.initials: A
authors.lastname: Pettenato
authors.affiliation: Lawrence Berkeley National Laboratory, Berkeley, California, USA.
authors.forename: Jayashree
authors.initials: J
authors.lastname: Ray
authors.affiliation: Lawrence Berkeley National Laboratory, Berkeley, California, USA.
authors.forename: R Jordan
authors.initials: RJ
authors.lastname: Waters
authors.affiliation: Lawrence Berkeley National Laboratory, Berkeley, California, USA.
authors.forename: Ryan A
authors.initials: RA
authors.lastname: Melnyk
authors.affiliation: Lawrence Berkeley National Laboratory, Berkeley, California, USA.
authors.forename: Romy
authors.initials: R
authors.lastname: Chakraborty
authors.affiliation: Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
authors.forename: Terry C
authors.identifier: http://orcid.org/0000-0002-2536-9993
authors.initials: TC
authors.lastname: Hazen
authors.affiliation: Lawrence Berkeley National Laboratory, Berkeley, California, USA.
authors.forename: Adam M
authors.initials: AM
authors.lastname: Deutschbauer
authors.affiliation: Lawrence Berkeley National Laboratory, Berkeley, California, USA.
authors.forename: Adam P
authors.initials: AP
authors.lastname: Arkin
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA adams@bmb.uga.edu.
authors.forename: Michael W W
authors.initials: MW
authors.lastname: Adams
mesh_terms: D003067:Coenzymes / Q000378:metabolism; D058440:Denitrification; D060587:Groundwater / Q000737:chemistry* / Q000382:microbiology*; D008982:Molybdenum / Q000378:metabolism*; D050901:Nitrate Reductase / Q000378:metabolism; D009566:Nitrates / Q000378:metabolism*; D044223:Pseudomonas stutzeri / Q000254:growth & development / Q000378:metabolism*; D013714:Tennessee
chemical_list: D003067:Coenzymes; D009566:Nitrates; D008982:Molybdenum; D050901:Nitrate Reductase