title: Structural analysis of novel trehalose-based oligosaccharides from extremely stress-tolerant ascospores of Neosartorya fischeri (Aspergillus fischeri).
abstract: Different fungi, including the genera Neosartorya, Byssochlamys and Talaromyces, produce (asco)spores that survive pasteurization treatments and are regarded as the most stress-resistant eukaryotic cells. Here, the NMR analysis of a series of trehalose-based oligosaccharides, being compatible solutes that are accumulated to high levels in ascospores of the fungus Neosartorya fischeri, is presented. These oligosaccharides consist of an α,α-trehalose backbone, extended with one [α-D-Glcp-(1 → 6)-α-D-Glcp-(1 ↔ 1)-α-D-Glcp; isobemisiose], two [α-D-Glcp-(1 → 6)-α-D-Glcp-(1 → 6)-α-D-Glcp-(1 ↔ 1)-α-D-Glcp] or three [α-D-Glcp-(1 → 6)-α-D-Glcp-(1 → 6)-α-D-Glcp-(1 → 6)-α-D-Glcp-(1 ↔ 1)-α-D-Glcp] glucose units. The tetra- and pentasaccharide, dubbed neosartose and fischerose, respectively, have not been reported before to occur in nature.
authors.affiliation: CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.
authors.forename: Timon T
authors.initials: TT
authors.lastname: Wyatt
authors.affiliation: NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
authors.forename: Gerrit J
authors.initials: GJ
authors.lastname: Gerwig
authors.affiliation: NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
authors.forename: Johannis P
authors.initials: JP
authors.lastname: Kamerling
authors.affiliation: Microbiology and Kluyver Centre for Genomics of Industrial Fermentation, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
authors.forename: Han A B
authors.initials: HA
authors.lastname: Wösten
authors.affiliation: CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands. Electronic address: j.dijksterhuis@cbs.knaw.nl.
authors.forename: Jan
authors.initials: J
authors.lastname: Dijksterhuis
mesh_terms: D001230:Aspergillus / Q000737:chemistry* / Q000502:physiology; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002850:Chromatography, Gel; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D013172:Spores, Fungal / Q000737:chemistry*; D014199:Trehalose / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D062610:Fungal Polysaccharides; D014199:Trehalose
keywords: Fischerose; Fungus; Isobemisiose; NMR spectroscopy; Neosartose; Trehalose