title: Developing a common bean core collection suitable for association mapping studies.
abstract: Because of the continuous introduction of germplasm from abroad, some collections have a high number of accessions, making it difficult to explore the genetic variability present in a germplasm bank for conservation and breeding purposes. Therefore, the aim of this study was to quantify and analyze the structure of genetic variability among 500 common bean accessions to construct a core collection. A total of 58 SSRs were used for this purpose. The polymorphism information content (PIC) in the 180 common bean accessions selected to compose the core collection ranged from 0.17 to 0.86, and the discriminatory power (DP) ranged from 0.21 to 0.90. The 500 accessions were clustered into 15 distinct groups and the 180 accessions into four distinct groups in the Structure analysis. According to analysis of molecular variance, the most divergent accessions comprised 97.2% of the observed genetic variability present within the base collection, confirming the efficiency of the selection criterion. The 180 selected accessions will be used for association mapping in future studies and could be potentially used by breeders to direct new crosses and generate elite cultivars that meet current and future global market needs.
authors.affiliation: Departamento de Genética e Evolução e Bioagentes, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, Brazil . ; Centro de Recursos Genéticos Vegetais, Instituto Agronômico de Campinas, Campinas, SP, Brazil .
authors.forename: Juliana Morini Küpper Cardoso
authors.initials: JM
authors.lastname: Perseguini
authors.affiliation: Centro de Recursos Genéticos Vegetais, Instituto Agronômico de Campinas, Campinas, SP, Brazil .
authors.forename: Gliciane Micaele Borges
authors.initials: GM
authors.lastname: Silva
authors.affiliation: Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Piracicaba, SP, Brazil .
authors.forename: João Ricardo Bachega Feijó
authors.initials: JR
authors.lastname: Rosa
authors.affiliation: Departamento de Biotecnologia Vegetal, Centro de Ciências Agrárias, Universidade Federal de São Carlos, Araras, SP, Brazil .
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Gazaffi
authors.affiliation: Centro de Recursos Genéticos Vegetais, Instituto Agronômico de Campinas, Campinas, SP, Brazil .
authors.forename: Jéssica Fernanda
authors.initials: JF
authors.lastname: Marçal
authors.affiliation: Centro de Grãos e Fibras, Instituto Agronômico de Campinas, Campinas, SP, Brazil.
authors.forename: Sérgio Augusto Morais
authors.initials: SA
authors.lastname: Carbonell
authors.affiliation: Centro de Grãos e Fibras, Instituto Agronômico de Campinas, Campinas, SP, Brazil.
authors.forename: Alisson Fernando
authors.initials: AF
authors.lastname: Chiorato
authors.affiliation: Agência Paulista de Tecnologia dos Agronegócios, Centro de Desenvolvimento Tecnológico, Instituto Agronômico, Campinas, SP, Brazil .
authors.forename: Maria Imaculada
authors.initials: MI
authors.lastname: Zucchi
authors.affiliation: Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Piracicaba, SP, Brazil .
authors.forename: Antonio Augusto Franco
authors.initials: AA
authors.lastname: Garcia
authors.affiliation: Departamento de Genética e Evolução e Bioagentes, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, Brazil . ; Centro de Recursos Genéticos Vegetais, Instituto Agronômico de Campinas, Campinas, SP, Brazil .
authors.forename: Luciana Lasry
authors.initials: LL
authors.lastname: Benchimol-Reis
keywords: Phaseolus vulgaris L; genetic diversity; genetic structure; microsatellites; molecular markers