title: Non-GMO genetically edited crop plants.
abstract: Direct delivery of purified Cas9 protein with guide RNA into plant cells, as opposed to plasmid-mediated delivery, displays high efficiency and reduced off-target effects. Following regeneration from edited cells, the ensuing plant is also likely to bypass genetically modified organism (GMO) legislation as the genome editing complex is degraded in the recipient cells.
authors.affiliation: Research and Innovation Centre, Fondazione Edmund Mach (FEM), Via Mach 1, 38010 San Michele all'Adige (TN), Italy. Electronic address: chidananda.nagamangala@fmach.it.
authors.forename: Chidananda Nagamangala
authors.initials: CN
authors.lastname: Kanchiswamy
authors.affiliation: Research and Innovation Centre, Fondazione Edmund Mach (FEM), Via Mach 1, 38010 San Michele all'Adige (TN), Italy.
authors.forename: Mickael
authors.initials: M
authors.lastname: Malnoy
authors.affiliation: Research and Innovation Centre, Fondazione Edmund Mach (FEM), Via Mach 1, 38010 San Michele all'Adige (TN), Italy.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Velasco
authors.affiliation: Center for Genome Engineering, Institute for Basic Science, Gwanak-ro 1, Gwanak-gu, Seoul 151-747, South Korea; Department of Chemistry, Seoul National University, Gwanak-ro 1, Gwanak-gu, Seoul 151-747, South Korea.
authors.forename: Jin-Soo
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Research and Innovation Centre, Fondazione Edmund Mach (FEM), Via Mach 1, 38010 San Michele all'Adige (TN), Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Viola
mesh_terms: D000383:Agriculture; D018556:Crops, Agricultural; D030821:Plants, Genetically Modified; D000094704:RNA, Guide, CRISPR-Cas Systems
chemical_list: D000094704:RNA, Guide, CRISPR-Cas Systems