title: DcpS is a transcript-specific modulator of RNA in mammalian cells.
abstract: The scavenger decapping enzyme DcpS is a multifunctional protein initially identified by its property to hydrolyze the resulting cap structure following 3' end mRNA decay. In Saccharomyces cerevisiae, the DcpS homolog Dcs1 is an obligate cofactor for the 5'-3' exoribonuclease Xrn1 while the Caenorhabditis elegans homolog Dcs-1, facilitates Xrn1 mediated microRNA turnover. In both cases, this function is independent of the decapping activity. Whether DcpS and its decapping activity can affect mRNA steady state or stability in mammalian cells remains unknown. We sought to determine DcpS target genes in mammalian cells using a cell-permeable DcpS inhibitor compound, RG3039 initially developed for therapeutic treatment of spinal muscular atrophy. Global mRNA levels were examined following DcpS decapping inhibition with RG3039. The steady-state levels of 222 RNAs were altered upon RG3039 treatment. Of a subset selected for validation, two transcripts that appear to be long noncoding RNAs HS370762 and BC011766, were dependent on DcpS and its scavenger decapping catalytic activity and referred to as DcpS-responsive noncoding transcripts (DRNT) 1 and 2, respectively. Interestingly, only the increase in DRNT1 transcript was accompanied with an increase of its RNA stability and this increase was dependent on both DcpS and Xrn1. Importantly, unlike in yeast where the DcpS homolog is an obligate cofactor for Xrn1, stability of additional Xrn1 dependent RNAs were not altered by a reduction in DcpS levels. Collectively, our data demonstrate that DcpS in conjunction with Xrn1 has the potential to regulate RNA stability in a transcript-selective manner in mammalian cells.
authors.affiliation: Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, New Jersey 08854, USA.
authors.forename: Mi
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, New Jersey 08854, USA.
authors.forename: Sophie
authors.initials: S
authors.lastname: Bail
authors.affiliation: Repligen Corporation, Waltham, Massachusetts 02453, USA.
authors.forename: Heather L
authors.initials: HL
authors.lastname: Plasterer
authors.affiliation: Repligen Corporation, Waltham, Massachusetts 02453, USA.
authors.forename: James
authors.initials: J
authors.lastname: Rusche
authors.affiliation: Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, New Jersey 08854, USA kiledjian@biology.rutgers.edu.
authors.forename: Megerditch
authors.initials: M
authors.lastname: Kiledjian
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers; D004722:Endoribonucleases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D006801:Humans; D011799:Quinazolines / Q000494:pharmacology; D020871:RNA Stability; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D022661:RNA, Untranslated / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C587914:5-((1-(2,6-dichlorobenzyl)piperidin-4-yl)methoxy)quinazoline-2,4-diamine; D017931:DNA Primers; D011799:Quinazolines; D012333:RNA, Messenger; D022661:RNA, Untranslated; D004722:Endoribonucleases; C485332:DcpS protein, human
keywords: DcpS; RNA decay; XRN1; lncRNA