title: A novel immunochromatographic test applied to a serological survey of Japanese encephalitis virus on pig farms in Korea.
abstract: Among vertebrate species, pigs are a major amplifying host of Japanese encephalitis virus (JEV) and measuring their seroconversion is a reliable indicator of virus activity. Traditionally, the hemagglutination inhibition test has been used for serological testing in pigs; however, it has several limitations and, thus, a more efficient and reliable replacement test is required. In this study, we developed a new immunochromatographic test for detecting antibodies to JEV in pig serum within 15 min. Specifically, the domain III region of the JEV envelope protein was successfully expressed in soluble form and used for developing the immunochromatographic test. The test was then applied to the surveillance of Japanese encephalitis (JE) in Korea. We found that our immunochromatographic test had good sensitivity (84.8%) and specificity (97.7%) when compared with an immunofluorescence assay used as a reference test. During the surveillance of JE in Korea in 2012, the new immunochromatographic test was used to test the sera of 1,926 slaughtered pigs from eight provinces, and 228 pigs (11.8%) were found to be JEV-positive. Based on these results, we also produced an activity map of JEV, which marked the locations of pig farms in Korea that tested positive for the virus. Thus, the immunochromatographic test reported here provides a convenient and effective tool for real-time monitoring of JEV activity in pigs.
authors.affiliation: Division of Arboviruses, National Institute of Health, Korea Centers for Disease Control and Prevention, Cheongju-si, Chungcheongbuk-do, Korea.
authors.forename: Go-Woon
authors.initials: GW
authors.lastname: Cha
authors.affiliation: Division of Arboviruses, National Institute of Health, Korea Centers for Disease Control and Prevention, Cheongju-si, Chungcheongbuk-do, Korea.
authors.forename: Eun Ju
authors.initials: EJ
authors.lastname: Lee
authors.affiliation: Gangwon Institute of Health and Environment, Shibuk-ro, Chuncheon-si, Gangwon-do, Korea.
authors.forename: Eun-Joo
authors.initials: EJ
authors.lastname: Lim
authors.affiliation: Chungbuk Institute of Health and Environment, Cheongju-si, Chungcheongbuk-do, Korea.
authors.forename: Kang Suk
authors.initials: KS
authors.lastname: Sin
authors.affiliation: Gyeongbuk Institute of Health and Environment, Yeongcheon-si, Gyeongsangbuk-do, Korea.
authors.forename: Woo Won
authors.initials: WW
authors.lastname: Park
authors.affiliation: Jeonnam Institute of Health and Environment, Muan-gun, Jeollanam-do, Korea.
authors.forename: Doo Young
authors.initials: DY
authors.lastname: Jeon
authors.affiliation: Division of Arboviruses, National Institute of Health, Korea Centers for Disease Control and Prevention, Cheongju-si, Chungcheongbuk-do, Korea.
authors.forename: Myung Guk
authors.initials: MG
authors.lastname: Han
authors.affiliation: Division of Arboviruses, National Institute of Health, Korea Centers for Disease Control and Prevention, Cheongju-si, Chungcheongbuk-do, Korea.
authors.forename: Won-Ja
authors.initials: WJ
authors.lastname: Lee
authors.affiliation: Division of Arboviruses, National Institute of Health, Korea Centers for Disease Control and Prevention, Cheongju-si, Chungcheongbuk-do, Korea.
authors.forename: Woo-Young
authors.initials: WY
authors.lastname: Choi
authors.affiliation: Division of Arboviruses, National Institute of Health, Korea Centers for Disease Control and Prevention, Cheongju-si, Chungcheongbuk-do, Korea; Department of Biomedical Sciences, Graduate School of Hallym University, Chuncheon-si, Gangwon-do, Korea.
authors.forename: Young Eui
authors.initials: YE
authors.lastname: Jeong
mesh_terms: D000822:Animal Husbandry; D000818:Animals; D015153:Blotting, Western; D002846:Chromatography, Affinity / Q000379:methods*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004664:Encephalitis Virus, Japanese / Q000276:immunology* / Q000302:isolation & purification; D005843:Geography; D017434:Protein Structure, Tertiary; D021122:Protein Subunits / Q000302:isolation & purification; D011933:Reagent Kits, Diagnostic; D011994:Recombinant Proteins / Q000378:metabolism; D056910:Republic of Korea; D012698:Serologic Tests / Q000379:methods*; D011795:Surveys and Questionnaires; D034421:Sus scrofa / Q000276:immunology* / Q000821:virology*; D014764:Viral Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D021122:Protein Subunits; D011933:Reagent Kits, Diagnostic; D011994:Recombinant Proteins; D014764:Viral Proteins