title: Development and evaluation of an immunochromatographic strip for rapid detection of capsid protein antigen p27 of avian leukosis virus.
abstract: A rapid immunochromatographic strip for detecting capsid protein antigen p27 of avian leukosis virus was successfully developed based on two high-affinity monoclonal antibodies. The test strip could detect not only 600pg purified recombinant p27 protein but also quantified avian leukosis virus as low as 70 TCID50, which has comparative sensitivity to the commercial enzyme-linked immunosorbent assay (ELISA) kit. For the evaluation of this test strip, 1100 samples consisting of cloacal swabs, meconium collected from the earliest stool of one day old chicken and virus isolates were assessed both by the strip and by the commercial ELISA kit. The agreement between these two tests was 93.91%, 93.42% and 100%, respectively. The sensitivity and specificity of the strip were also calculated by using the ELISA kit as the standard. This immunochromatographic strip provides advantages of rapid and simple detection of capsid protein antigen p27 of avian leukosis virus, which could be applied as an on-site testing assay and used for control and eradication programs of avian leukosis disease.
authors.affiliation: Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, Yangzhou 225009, China; Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou 225009, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Qian
authors.affiliation: Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, Yangzhou 225009, China.
authors.forename: You-zhi
authors.initials: YZ
authors.lastname: Liang
authors.affiliation: Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, Yangzhou 225009, China.
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Yin
authors.affiliation: Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, Yangzhou 225009, China; Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou 225009, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.
authors.forename: Hong-xia
authors.initials: HX
authors.lastname: Shao
authors.affiliation: Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, Yangzhou 225009, China; Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou 225009, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.
authors.forename: Jian-qiang
authors.initials: JQ
authors.lastname: Ye
authors.affiliation: Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, Yangzhou 225009, China; Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou 225009, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China. Electronic address: aijian@yzu.edu.cn.
authors.forename: Ai-jian
authors.initials: AJ
authors.lastname: Qin
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology; D001353:Avian Leukosis / Q000175:diagnosis* / Q000821:virology; D001354:Avian Leukosis Virus / Q000276:immunology / Q000302:isolation & purification*; D036022:Capsid Proteins / Q000032:analysis* / Q000276:immunology; D002645:Chickens; D002846:Chromatography, Affinity / Q000379:methods*; D011201:Poultry Diseases / Q000175:diagnosis* / Q000821:virology; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins
keywords: Avian leukosis virus; Capsid protein antigen p27; Enzyme-linked immunosorbent assay; Immunochromatographic strip