title: [Association of polymorphism in the promoter region of PCA3 gene with risk of prosate cancer].
abstract: OBJECTIVE
  To investigate the polymorphism in the promoter region of PCA3 gene and its relationship with risk of prostate cancer (PCa).
  
  
  METHODS
  The promoter region of PCA3 gene of the DNA of peripheral blood mononuclear cells was detected by sequence analysis in the 186 PCa and 141 BPH patients and 135 healthy control individuals. If the samples were detected with polymorphism of insection/deletion, clone sequence analysis was used with pBS-T carrier to verify it.
  
  
  RESULTS
  There were 5 polymorphisms. TAAA repeat times: 4, 5, 6, 7, 8, and 8 genotypes (TAAA 4/5, TAAA 4/6, TAAA 5/5, TAAA 5/6, TAAA 5/7, TAAA 5/8, TAAA 6/6, and TAAA 6/7) were detected in the promoter region of PCA3 gene. The eight genotypes were divided into three groups: ≤10TAAA, 11TAAA, ≥12TAAA. Unconditional logistic regression analysis models were used to analyze the relationship between different genotypes and cancer risks adjusted by sex and age. The type 11TAAA and ≥12TAAA was associated with higher relative risk for prostate cancer than the group ≤10TAAA [OR=1.74, 95% CI=1.06-2.87 (for type 11TAAA); OR=5.63, 95% CI=1.85-17.19 (for type ≥12TAAA)]. In the 186 PCa patients, there was 62.4% allele of PCA3 gene with AG/CA mutation found in the promoter 18-19 bp region of PCA3 gene and it had a close relation with the development of prostate cancer.
  
  
  CONCLUSIONS
  Short tandem repeats are found in the promoter region of the PCA3 gene in PCa patients, and the increase of TAAA repeat sequences highly enhance the relative risk of prostate cancer development. The occurrence of such STR might be related to the mutations in their upstream loci.
authors.affiliation: Department of Clinical Laboratory Medicine, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325005, China; Email: gonewithwind95@163.com.
authors.forename: Wu
authors.initials: W
authors.lastname: Zhou
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Tao
authors.forename: Zhongyong
authors.initials: Z
authors.lastname: Wang
authors.forename: Zhanguo
authors.initials: Z
authors.lastname: Chen
authors.forename: Mo
authors.initials: M
authors.lastname: Shen
authors.forename: Qiyu
authors.initials: Q
authors.lastname: Xu
authors.forename: Haixiao
authors.initials: H
authors.lastname: Xie
authors.forename: Zhixian
authors.initials: Z
authors.lastname: Yu
authors.forename: Guorong
authors.initials: G
authors.lastname: Chen
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D052138:Genes, Neoplasm / Q000502:physiology*; D005838:Genotype; D006801:Humans; D007963:Leukocytes, Mononuclear; D008297:Male; D018895:Microsatellite Repeats; D009154:Mutation; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D011471:Prostatic Neoplasms / Q000453:epidemiology / Q000235:genetics*; D012306:Risk
chemical_list: D000951:Antigens, Neoplasm; C479341:prostate cancer antigen 3, human