title: Effect of Variants of Penicillin-Binding Protein 2 on Cephalosporin and Carbapenem Susceptibilities in Neisseria gonorrhoeae.
abstract: To characterize the relationship between penicillin-binding protein 2 (PBP2/penA) and susceptibility to extended-spectrum cephalosporins (ESCs) and carbapenem antibiotics, we compared 17 PBP2 variants in Neisseria gonorrhoeae. Nonmosaic and mosaic variants of PBP2 caused decreased susceptibility to ESCs and, to a lesser extent, to carbapenems. An A501P substitution in mosaic XXXIV_A501P conferred decreased susceptibility to ESCs but restored carbapenem susceptibility to wild-type levels. These results could aid the molecular surveillance of antimicrobial resistance to these agents.
authors.affiliation: Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
authors.forename: Amrita
authors.initials: A
authors.lastname: Bharat
authors.affiliation: Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
authors.forename: Walter
authors.initials: W
authors.lastname: Demczuk
authors.affiliation: Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
authors.forename: Irene
authors.initials: I
authors.lastname: Martin
authors.affiliation: Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada michael.mulvey@phac-aspc.gc.ca.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Mulvey
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015780:Carbapenems / Q000494:pharmacology*; D002352:Carrier Proteins / Q000235:genetics*; D002511:Cephalosporins / Q000494:pharmacology*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D014644:Genetic Variation; D006069:Gonorrhea / Q000188:drug therapy; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009344:Neisseria gonorrhoeae / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D046929:Serine-Type D-Ala-D-Ala Carboxypeptidase
chemical_list: D000900:Anti-Bacterial Agents; D015780:Carbapenems; D002352:Carrier Proteins; D002511:Cephalosporins; D046929:Serine-Type D-Ala-D-Ala Carboxypeptidase; C586047:penicillin-binding protein 2, Neisseria gonorrhoeae