title: Availability of extracellular matrix biopolymers and differentiation state of human mesenchymal stem cells determine tissue-like growth in vitro.
abstract: To explore the space-filling growth of adherent mesenchymal stem cells (MSC) into tissue-like structures in vitro, human bone marrow derived MSC were exposed to fibronectin-coated, millimeter-sized, triangular channels casted in poly(dimethyl siloxane) carriers. The results revealed that the three dimensional (3D) growth of MSC differs in dependence on differentiation status and availability of extracellular matrix (ECM) proteins: Massive 3D structure formation was observed for MSC under pro-osteogenic stimulation but not for undifferentiated MSC nor for MSC under pro-adipogenic stimulation; boosting cellular matrix secretion and addition of soluble ECM proteins caused extensive 3D tissue formation of undifferentiated MSC. The reported findings may contribute to bridge the gap between in vitro and in vivo analyses and guide the application of MSC in tissue replacement approaches.
authors.affiliation: Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials, Dresden, Germany.
authors.forename: Manuela
authors.initials: M
authors.lastname: Herklotz
authors.affiliation: Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials, Dresden, Germany.
authors.forename: Marina C
authors.initials: MC
authors.lastname: Prewitz
authors.affiliation: Max Planck Institute of Colloids and Interfaces, Department of Biomaterials, Potsdam, Germany.
authors.forename: Cécile M
authors.initials: CM
authors.lastname: Bidan
authors.affiliation: Max Planck Institute of Colloids and Interfaces, Department of Biomaterials, Potsdam, Germany.
authors.forename: John W C
authors.initials: JW
authors.lastname: Dunlop
authors.affiliation: Max Planck Institute of Colloids and Interfaces, Department of Biomaterials, Potsdam, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Fratzl
authors.affiliation: Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials, Dresden, Germany; Center for Regenerative Therapies Dresden, TU Dresden, Dresden, Germany. Electronic address: werner@ipfdd.de.
authors.forename: Carsten
authors.initials: C
authors.lastname: Werner
mesh_terms: D000199:Actins / Q000378:metabolism; D050156:Adipogenesis; D000328:Adult; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D004867:Equipment Design; D005109:Extracellular Matrix / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D055767:Immobilized Proteins / Q000378:metabolism; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D010012:Osteogenesis; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods; D055815:Young Adult
chemical_list: D000199:Actins; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D055767:Immobilized Proteins
keywords: Extracellular matrix; Mesenchymal stem cells; Three-dimensional growth