title: Enhanced production of porcine circovirus type 2 (PCV2) virus-like particles in Sf9 cells by translational enhancers.
abstract: OBJECTIVE
  To investigate the effect of three translational enhancers for enhancing transgene expression in baculovirus expression vector system using GFP as a reporter gene and selected translational enhancers to increase porcine circovirus type 2 (PCV2) VLPs production.
  
  
  RESULTS
  P10UTR (the 3'-untranslated region from the baculovirus p10 gene), Syn21 (a synthetic AT-rich 21-bp sequence) and P10UTR/Syn21 increased the GFP yield by 1.4-, 4- and 4.8-fold, respectively. While IVS (intron from Drosophila myosin heavy chain gene) decreased the GFP yield by 65%. Moreover, the synergy of P10UTR/Syn21 increased the yield of PCV2 VLPs by 4.1 fold (45 μg/10(6) cells) compared with standard baculovirus vector.
  
  
  CONCLUSIONS
  The synergy of P10UTR/Syn21 is a potential strategy to improve the recombinant vaccine production besides PCV2 VLPs in BEVS.
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, People's Republic of China, lyk19861211@163.com.
authors.forename: Yangkun
authors.initials: Y
authors.lastname: Liu
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Lunguang
authors.initials: L
authors.lastname: Yao
authors.forename: Huafang
authors.initials: H
authors.lastname: Hao
authors.forename: Xiangjing
authors.initials: X
authors.lastname: Fu
authors.forename: Zengqi
authors.initials: Z
authors.lastname: Yang
authors.forename: Enqi
authors.initials: E
authors.lastname: Du
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics / Q000378:metabolism; D036022:Capsid Proteins / Q000235:genetics* / Q000378:metabolism; D017925:Circovirus / Q000235:genetics* / Q000378:metabolism; D004742:Enhancer Elements, Genetic; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D061987:Sf9 Cells; D013552:Swine; D058425:Vaccines, Virus-Like Particle / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D036022:Capsid Proteins; D058425:Vaccines, Virus-Like Particle; D014764:Viral Proteins; D049452:Green Fluorescent Proteins; C078072:p10 protein, Baculovirus