title: The Protecting Effect of Deoxyschisandrin and Schisandrin B on HaCaT Cells against UVB-Induced Damage.
abstract: Schisandra chinensis is a traditional Chinese medicine that has multiple biological activities, including antioxidant, anticancer, tonic, and anti-aging effects. Deoxyschisandrin (SA) and schisandrin B (SB), the two major lignans isolated from S. chinensis, exert high antioxidant activities in vitro and in vivo by scavenging free radicals, such as reactive oxygen species (ROS). Ultraviolet B-ray (UVB) radiation induces the production of ROS and DNA damage, which eventually leads to cell death by apoptosis. However, it is unknown whether SA or SB protects cells against UVB-induced cellular DNA damage. Our study showed that both SA and SB effectively protected HaCaT cells from UVB-induced cell death by antagonizing UVB-mediated production of ROS and induction of DNA damage. Our results showed that both SA and SB significantly prevented UVB-induced loss of cell viability using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assays. Dichloro-dihydro-fluorescein diacetate (DCFH-DA) assays showed that the production of ROS following UVB exposure was inhibited by treatment with SA and SB. Moreover, SA and SB decreased the UVB-induced DNA damage in HaCaT cells by comet assays. In addition, SA and SB also prevented UVB-induced cell apoptosis and the cleavage of caspase-3, caspase-8 and caspase-9. In a word, our results imply that the antioxidants SA and SB could protect cells from UVB-induced cell damage via scavenging ROS.
authors.affiliation: Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Hou
authors.affiliation: Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Gao
authors.affiliation: Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, China.
authors.forename: Datao
authors.initials: D
authors.lastname: Wang
authors.affiliation: Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, China.
authors.forename: Qingxiu
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, China.
authors.forename: Siwen
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, China.
authors.forename: Yingping
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D020169:Caspases / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D034242:Cyclooctanes / Q000494:pharmacology*; D019610:Cytoprotection / Q000187:drug effects*; D004249:DNA Damage / Q000187:drug effects*; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism; D017705:Lignans / Q000494:pharmacology*; D011083:Polycyclic Compounds / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D032141:Schisandra / Q000737:chemistry; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D000975:Antioxidants; D034242:Cyclooctanes; D017705:Lignans; D011083:Polycyclic Compounds; D017382:Reactive Oxygen Species; C015499:schizandrin B; C034734:schizandrin A; D020169:Caspases