title: Synaptic molecular imaging in spared and deprived columns of mouse barrel cortex with array tomography.
abstract: A major question in neuroscience is how diverse subsets of synaptic connections in neural circuits are affected by experience dependent plasticity to form the basis for behavioral learning and memory. Differences in protein expression patterns at individual synapses could constitute a key to understanding both synaptic diversity and the effects of plasticity at different synapse populations. Our approach to this question leverages the immunohistochemical multiplexing capability of array tomography (ATomo) and the columnar organization of mouse barrel cortex to create a dataset comprising high resolution volumetric images of spared and deprived cortical whisker barrels stained for over a dozen synaptic molecules each. These dataset has been made available through the Open Connectome Project for interactive online viewing, and may also be downloaded for offline analysis using web, Matlab, and other interfaces.
authors.affiliation: Graduate Program in Neurosciences, Stanford University School of Medicine , Stanford, California 94305, USA ; Department of Molecular and Cellular Physiology, Stanford University School of Medicine , Stanford, California 94305, USA.
authors.forename: Nicholas C
authors.identifier: 0000-0001-6750-9248
authors.initials: NC
authors.lastname: Weiler
authors.affiliation: Department of Molecular and Cellular Physiology, Stanford University School of Medicine , Stanford, California 94305, USA ; Allen Institute for Brain Science , Seattle, Washington 98103, USA.
authors.forename: Forrest
authors.initials: F
authors.lastname: Collman
authors.affiliation: Department of Statistical Science, Duke University , Durham, North Carolina 27708, USA ; Department of Biomedical Engineering, The Johns Hopkins University , Baltimore, Maryland 21218, USA.
authors.forename: Joshua T
authors.initials: JT
authors.lastname: Vogelstein
authors.affiliation: Department of Computer Science, The Johns Hopkins University , Baltimore, Maryland 21218, USA.
authors.forename: Randal
authors.initials: R
authors.lastname: Burns
authors.affiliation: Department of Molecular and Cellular Physiology, Stanford University School of Medicine , Stanford, California 94305, USA ; Allen Institute for Brain Science , Seattle, Washington 98103, USA.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Smith
mesh_terms: D000818:Animals; D007858:Learning; D008568:Memory; D051379:Mice; D009473:Neuronal Plasticity; D013003:Somatosensory Cortex / Q000737:chemistry* / Q000502:physiology; D013569:Synapses / Q000737:chemistry* / Q000502:physiology; D014057:Tomography, X-Ray Computed