title: Impact of graphene oxide on viability of Chinese hamster ovary and mouse hepatoma MH-22A cells.
abstract: The evaluation of the cyto- and bio-compatibility is a critical step in the development of graphene oxide (GO) as a new promising material for in vivo biomedical applications. In this study, we report the impact of GO, with and without the addition of bovine serum albumin, on healthy (Chinese hamster ovary) and a cancer (mouse hepatoma MH-22A) cells viability and the estimation of the intracellular distribution of GO inside the cells in vitro. The viability tests were performed using a colony formation assay. The intracellular distribution of GO was estimated using Raman spectroscopy and imaging. The viability of both cell lines decreased with increasing concentration of graphene oxide (12.5-50.0 μg/ml): in the case of Chinese hamster ovary cells viability decreased from 44% to 11%, in the case of mouse hepatoma MH-22A cells--from 22% to 3%. These cell lines significantly differed in their response to GO and GO-BSA formulations. The results of viability tests correlate with results of atomic force microscopy and Raman spectroscopy and imaging findings. The GO influence on cell morphology changes, cell structure, cells colony growth dynamics and GO accumulation inside the cells was higher in the case of mouse hepatoma MH-22A cells.
authors.affiliation: Research Center for Microsystems and Nanotechnology, Kaunas University of Technology, 65 Studentu str., Kaunas LT-51369, Lithuania; Department of Biology, Faculty of Natural Sciences, Vytautas Magnus University, 58 K. Donelaicio str., Kaunas LT-44248, Lithuania.
authors.forename: Danute
authors.initials: D
authors.lastname: Batiuskaite
authors.affiliation: Research Center for Microsystems and Nanotechnology, Kaunas University of Technology, 65 Studentu str., Kaunas LT-51369, Lithuania. Electronic address: nora.grinceviciute@ktu.lt.
authors.forename: Nora
authors.initials: N
authors.lastname: Grinceviciute
authors.affiliation: Research Center for Microsystems and Nanotechnology, Kaunas University of Technology, 65 Studentu str., Kaunas LT-51369, Lithuania. Electronic address: vsnitka@ktu.lt.
authors.forename: Valentinas
authors.initials: V
authors.lastname: Snitka
mesh_terms: D000818:Animals; D016466:CHO Cells; D006528:Carcinoma, Hepatocellular; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D006108:Graphite / Q000633:toxicity*; D008113:Liver Neoplasms; D051379:Mice; D010087:Oxides / Q000633:toxicity*; D012710:Serum Albumin, Bovine / Q000494:pharmacology
chemical_list: D010087:Oxides; D012710:Serum Albumin, Bovine; D006108:Graphite
keywords: AFM; Chinese hamster ovary (CHO) cells; Graphene oxide; Mouse hepatoma MH-22A cells; Raman spectroscopy and imaging; Viability