title: A receptor pair with an integrated decoy converts pathogen disabling of transcription factors to immunity.
abstract: Microbial pathogens infect host cells by delivering virulence factors (effectors) that interfere with defenses. In plants, intracellular nucleotide-binding/leucine-rich repeat receptors (NLRs) detect specific effector interference and trigger immunity by an unknown mechanism. The Arabidopsis-interacting NLR pair, RRS1-R with RPS4, confers resistance to different pathogens, including Ralstonia solanacearum bacteria expressing the acetyltransferase effector PopP2. We show that PopP2 directly acetylates a key lysine within an additional C-terminal WRKY transcription factor domain of RRS1-R that binds DNA. This disrupts RRS1-R DNA association and activates RPS4-dependent immunity. PopP2 uses the same lysine acetylation strategy to target multiple defense-promoting WRKY transcription factors, causing loss of WRKY-DNA binding and transactivating functions needed for defense gene expression and disease resistance. Thus, RRS1-R integrates an effector target with an NLR complex at the DNA to switch a potent bacterial virulence activity into defense gene activation.
authors.affiliation: INRA, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR441, Castanet-Tolosan 31326, France; CNRS, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR2594, Castanet-Tolosan 31326, France; Department of Plant-Microbe Interactions, Max Planck Institute for Plant Breeding Research, Carl-von-Linne-Weg 10, Köln 50829, Germany.
authors.forename: Clémentine
authors.initials: C
authors.lastname: Le Roux
authors.affiliation: INRA, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR441, Castanet-Tolosan 31326, France; CNRS, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR2594, Castanet-Tolosan 31326, France.
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Huet
authors.affiliation: Institut Fédératif de Recherche 3450, Plateforme Imagerie, Pôle de Biotechnologie Végétale, Castanet-Tolosan 31326, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Jauneau
authors.affiliation: Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, UPS, UMR 5546, BP 42617 Auzeville, Castanet-Tolosan 31326, France; CNRS, UMR 5546, BP 42617, Castanet-Tolosan 31326, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Camborde
authors.affiliation: INRA, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR441, Castanet-Tolosan 31326, France; CNRS, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR2594, Castanet-Tolosan 31326, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Trémousaygue
authors.affiliation: Université Grenoble Alpes, iRTSV-BGE, Grenoble 38000, France; CEA, iRTSV-BGE, Grenoble 38000, France; INSERM, BGE, Grenoble 38000, France.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Kraut
authors.affiliation: INRA, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR441, Castanet-Tolosan 31326, France; CNRS, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR2594, Castanet-Tolosan 31326, France.
authors.forename: Binbin
authors.initials: B
authors.lastname: Zhou
authors.affiliation: INRA, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR441, Castanet-Tolosan 31326, France; CNRS, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR2594, Castanet-Tolosan 31326, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Levaillant
authors.affiliation: Defense in Plant-Pathogen Interactions, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Adachi
authors.affiliation: Defense in Plant-Pathogen Interactions, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Yoshioka
authors.affiliation: INRA, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR441, Castanet-Tolosan 31326, France; CNRS, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR2594, Castanet-Tolosan 31326, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Raffaele
authors.affiliation: INRA, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR441, Castanet-Tolosan 31326, France; CNRS, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR2594, Castanet-Tolosan 31326, France.
authors.forename: Richard
authors.initials: R
authors.lastname: Berthomé
authors.affiliation: Université Grenoble Alpes, iRTSV-BGE, Grenoble 38000, France; CEA, iRTSV-BGE, Grenoble 38000, France; INSERM, BGE, Grenoble 38000, France.
authors.forename: Yohann
authors.initials: Y
authors.lastname: Couté
authors.affiliation: Department of Plant-Microbe Interactions, Max Planck Institute for Plant Breeding Research, Carl-von-Linne-Weg 10, Köln 50829, Germany.
authors.forename: Jane E
authors.initials: JE
authors.lastname: Parker
authors.affiliation: INRA, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR441, Castanet-Tolosan 31326, France; CNRS, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR2594, Castanet-Tolosan 31326, France. Electronic address: laurent.deslandes@toulouse.inra.fr.
authors.forename: Laurent
authors.initials: L
authors.lastname: Deslandes
mesh_terms: D000123:Acetyltransferases / Q000378:metabolism; D017360:Arabidopsis / Q000276:immunology* / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D008958:Models, Molecular; D010940:Plant Proteins / Q000378:metabolism; D043368:Ralstonia solanacearum / Q000201:enzymology / Q000378:metabolism / Q000472:pathogenicity; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D010940:Plant Proteins; C582898:RRS1 protein, Arabidopsis; D014157:Transcription Factors; C097421:rps4 protein, plant; D004247:DNA; D000123:Acetyltransferases