title: Salvianolic acid B attenuates apoptosis and inflammation via SIRT1 activation in experimental stroke rats.
abstract: Silent information regulator 1 (SIRT1), a histone deacetylase, has been suggested to be effective in ischemic brain diseases. Salvianolic acid B (SalB) is a polyphenolic and one of the active components of Salvia miltiorrhiza Bunge. Previous studies suggested that SalB is protective against ischemic stroke. However, the role of SIRT1 in the protective effect of SalB against cerebral ischemia has not been explored. In this study, the rat brain was subjected to middle cerebral artery occlusion (MCAO). Before this surgery, rats were intraperitoneally administrated SalB with or without EX527, a specific SIRT1 inhibitor. The infarct volume, neurological score and brain water content were assessed. In addition, levels of TNF-α and IL-1β in the brain tissues were detected by commercial ELISA kits. And the expression levels of SIRT, Ac-FOXO1, Bcl-2 and Bax were detected by Western blot. The results suggested that SalB exerted a cerebral-protective effect, as shown by reduced infarct volume, lowered brain edema and increased neurological scores. SalB also exerted anti-inflammatory effects as indicated by the decreased TNF-α and IL-1β levels in the brain tissue. Moreover, SalB upregulated the expression of SIRT1 and Bcl-2 and downregulated the expression of Ac-FOXO1 and Bax. These effects of SalB were abolished by EX527 treatment. In summary, our results demonstrate that SalB treatment attenuates brain injury induced by ischemic stoke via reducing apoptosis and inflammation through the activation of SIRT1 signaling.
authors.affiliation: Department of Cardiology, No. 371 Central Hospital of PLA, Xinxiang, Henan 453000, China.
authors.forename: Hongdi
authors.initials: H
authors.lastname: Lv
authors.affiliation: Department of Nursing, No. 371 Central Hospital of PLA, Xinxiang, Henan 453000, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Interventional Radiology, No. 371 Central Hospital of PLA, Xinxiang, Henan 453000, China. Electronic address: shenjinchang2014@163.com.
authors.forename: Jinchang
authors.initials: J
authors.lastname: Shen
authors.affiliation: Department of Drugs and Equipment, No. 371 Central Hospital of PLA, Xinxiang, Henan 453000, China.
authors.forename: Shaojun
authors.initials: S
authors.lastname: Hao
authors.affiliation: Department of Urology, No. 371 Central Hospital of PLA, Xinxiang, Henan 453000, China.
authors.forename: Aimin
authors.initials: A
authors.lastname: Ming
authors.affiliation: Department of Drugs and Equipment, No. 371 Central Hospital of PLA, Xinxiang, Henan 453000, China.
authors.forename: Xidong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Cardiology, No. 371 Central Hospital of PLA, Xinxiang, Henan 453000, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Su
authors.affiliation: Department of Drugs and Equipment, No. 371 Central Hospital of PLA, Xinxiang, Henan 453000, China.
authors.forename: Zhengchen
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D001572:Benzofurans / Q000737:chemistry / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D001929:Brain Edema / Q000188:drug therapy / Q000473:pathology / Q000503:physiopathology; D002545:Brain Ischemia / Q000188:drug therapy / Q000473:pathology / Q000503:physiopathology; D002227:Carbazoles / Q000494:pharmacology; D002491:Central Nervous System Agents / Q000494:pharmacology; D004195:Disease Models, Animal; D020244:Infarction, Middle Cerebral Artery; D007249:Inflammation / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D008297:Male; D018696:Neuroprotective Agents / Q000737:chemistry / Q000494:pharmacology*; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D012720:Severity of Illness Index; D056564:Sirtuin 1 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D020521:Stroke / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D016896:Treatment Outcome; D053630:Depsides
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D001572:Benzofurans; D002227:Carbazoles; D002491:Central Nervous System Agents; D018696:Neuroprotective Agents; D056564:Sirtuin 1; C550547:6-chloro-2,3,4,9-tetrahydro-1H-carbazole-1-carboxamide; C076944:salvianolic acid B; D053630:Depsides; C488368:Sirt1 protein, rat
keywords: Apoptosis; Inflammation; Ischemic stroke; SIRT1; Salvianolic acid B