title: Encapsulated boron as an osteoinductive agent for bone scaffolds.
abstract: The aim of this study was to develop boron (B)-releasing polymeric scaffold to promote regeneration of bone tissue. Boric acid-doped chitosan nanoparticles with a diameter of approx. 175 nm were produced by tripolyphosphate (TPP)-initiated ionic gelation process. The nanoparticles strongly attached via electrostatic interactions into chitosan scaffolds produced by freeze-drying with approx. 100 μm pore diameter. According to the ICP-OES results, following first 5h initial burst release, fast release of B from scaffolds was observed for 24h incubation period in conditioned medium. Then, slow release of B was performed over 120 h. The results of the cell culture studies proved that the encapsulated boron within the scaffolds can be used as an osteoinductive agent by showing its positive effects on the proliferation and differentiation of MC3T3-E1 preosteoblastic cells.
authors.affiliation: Hacettepe University, Department of Bioengineering, Ankara, Turkey. Electronic address: menemse@gmail.com.
authors.forename: Menemşe
authors.initials: M
authors.lastname: Gümüşderelioğlu
authors.affiliation: Hacettepe University, Department of Bioengineering, Ankara, Turkey.
authors.forename: Ekin Ö
authors.initials: EÖ
authors.lastname: Tunçay
authors.affiliation: Hacettepe University, Department of Bioengineering, Ankara, Turkey.
authors.forename: Gökçe
authors.initials: G
authors.lastname: Kaynak
authors.affiliation: Hacettepe University, Department of Bioengineering, Ankara, Turkey.
authors.forename: Tolga T
authors.initials: TT
authors.lastname: Demirtaş
authors.affiliation: Bülent Ecevit University, Department of Biomedical Engineering, Zonguldak, Turkey.
authors.forename: Seda Tığlı
authors.initials: ST
authors.lastname: Aydın
authors.affiliation: Selcuk University, Faculty of Dentistry, Department of Periodontology, Konya, Turkey.
authors.forename: Sema S
authors.initials: SS
authors.lastname: Hakkı
mesh_terms: D000327:Adsorption; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D050071:Bone Density Conservation Agents / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology; D001861:Bone Regeneration / Q000187:drug effects; D001888:Boric Acids / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D001895:Boron / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry; D003692:Delayed-Action Preparations / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D004339:Drug Compounding; D016503:Drug Delivery Systems; D065546:Drug Liberation; D051379:Mice; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D010012:Osteogenesis / Q000187:drug effects*; D010316:Particle Size; D016062:Porosity; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D015415:Biomarkers; D050071:Bone Density Conservation Agents; D001888:Boric Acids; D003692:Delayed-Action Preparations; D048271:Chitosan; D001895:Boron; C032688:boric acid
keywords: Bone tissue engineering; Boron; Chitosan; Nanoparticle; Scaffold