title: Contribution of PRS3, RPB4 and ZWF1 to the resistance of industrial Saccharomyces cerevisiae CCUG53310 and PE-2 strains to lignocellulosic hydrolysate-derived inhibitors.
abstract: PRS3, RPB4 and ZWF1 were previously identified as key genes for yeast tolerance to lignocellulose-derived inhibitors. To better understand their contribution to yeast resistance to the multiple stresses occurring during lignocellulosic hydrolysate fermentations, we overexpressed these genes in two industrial Saccharomyces cerevisiae strains, CCUG53310 and PE-2, and evaluated their impact on the fermentation of Eucalyptus globulus wood and corn cob hydrolysates. PRS3 overexpression improved the fermentation rate (up to 32%) and productivity (up to 48%) in different hydrolysates. ZWF1 and RPB4 overexpression did not improve the fermentation performance, but their increased expression in the presence of acetic acid, furfural and hydroxymethylfurfural was found to contribute to yeast adaptation to these inhibitors. This study expands our understanding about the molecular mechanisms involved in industrial yeast tolerance to the stresses occurring during lignocellulosic bioethanol production and highlights the importance of selecting appropriate strain backgrounds/hydrolysates combinations when addressing further improvement of these processes.
authors.affiliation: CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.
authors.forename: Joana T
authors.initials: JT
authors.lastname: Cunha
authors.affiliation: CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.
authors.forename: Tatiana Q
authors.initials: TQ
authors.lastname: Aguiar
authors.affiliation: CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.
authors.forename: Aloia
authors.initials: A
authors.lastname: Romaní
authors.affiliation: CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.
authors.forename: Carla
authors.initials: C
authors.lastname: Oliveira
authors.affiliation: CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal. Electronic address: luciliad@deb.uminho.pt.
authors.forename: Lucília
authors.initials: L
authors.lastname: Domingues
mesh_terms: D019342:Acetic Acid / Q000494:pharmacology; D005052:Eucalyptus / Q000187:drug effects / Q000378:metabolism; D005285:Fermentation / Q000187:drug effects; D005656:Fungal Proteins / Q000378:metabolism*; D005662:Furaldehyde / Q000031:analogs & derivatives / Q000494:pharmacology; D006868:Hydrolysis; D008031:Lignin / Q000494:pharmacology*; D012319:RNA Polymerase II / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D013312:Stress, Physiological / Q000187:drug effects*
chemical_list: D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; C036909:lignocellulose; C008046:5-hydroxymethylfurfural; D008031:Lignin; D005662:Furaldehyde; D012319:RNA Polymerase II; C423309:RPB4 protein, S cerevisiae; D019342:Acetic Acid
keywords: Expression and overexpression analysis; Industrial Saccharomyces cerevisiae; Lignocellulose-derived inhibitors; PRS3, RPB4 and ZWF1; Second generation bioethanol