title: Angiopoietin-like protein 2 may mediate the inflammation in murine mastitis through the activation of interleukin-6 and tumour necrosis factor-α.
abstract: Mastitis is the inflammation of the mammary gland. Recent research has shown that Angiopoietin-like protein 2 (ANGPTL2) is a key inflammatory mediator. In the present study, we tested whether there is a correlation between increased ANGPTL2 expression and inflammation in response to Staphylococcus aureus in murine mastitis and the mechanisms involved. Thirty mice were divided into two groups: blank control group, challenged group. The entire infused mammary glands were removed to observe the changes of histopathology, myeloperoxidase (MPO) activity, production of tumour necrosis factor-α (TNF-α) and interleukin (IL)-6, and genes expression of ANGPTL2, TNF-α and IL-6. In challenged group, the structure of mammary glands was damaged and the large areas of cell fragments were observed. The MPO activity, IL-6 and TNF-α concentrations, ANGPTL2, IL-6, and TNF-α mRNA levels were significantly elevated in challenged group compared with blank control group. The present findings indicate ANGPTL2 may mediate the inflammation in murine mastitis through the activation of IL-6 and TNF-α.
authors.affiliation: College of Veterinary Medicine, Hunan Agricultural University, Furong District, Changsha, 410128, China.
authors.forename: Ji-Ying
authors.initials: JY
authors.lastname: Wang
authors.forename: Hong-Bo
authors.initials: HB
authors.lastname: Xiao
authors.forename: Zhi-Liang
authors.initials: ZL
authors.lastname: Sun
authors.forename: Da-Sheng
authors.initials: DS
authors.lastname: Zhang
mesh_terms: D000091302:Angiopoietin-Like Protein 2; D000075802:Angiopoietin-like Proteins; D042682:Angiopoietins / Q000235:genetics / Q000276:immunology*; D000818:Animals; D005260:Female; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics* / Q000276:immunology; D008413:Mastitis / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D051379:Mice; D013203:Staphylococcal Infections / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics* / Q000276:immunology
chemical_list: D000091302:Angiopoietin-Like Protein 2; D000075802:Angiopoietin-like Proteins; D042682:Angiopoietins; C485793:Angptl2 protein, mouse; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha