title: Protective effects of tirofiban on ischemia/reperfusion-induced renal injury in vivo and in vitro.
abstract: Tirofiban, a glycoprotein IIb/IIIa receptor inhibitor, is widely used in the management of patients with unstable angina or myocardial infarction, and shows protective effects on ischemia/reperfusion (I/R) injured heart. Whether or not it has protective effect on I/R injured kidney is not known. The present in vivo and in vitro study found that serum creatinine (SCR) and blood urea nitrogen (BUN) were significantly increased in I/R rats, accompanied by histopathological damage of the kidney. Apoptotic cells, leukocyte infiltration and ROS production were increased in I/R rats. Pretreatment by intravenous injection of tirofiban (200μg/kg) reduced SCR and BUN levels, ameliorated renal histopathological changes, and decreased ROS production, cell apoptosis and leukocyte infiltration in I/R injured kidney. Our further study showed that the protection of tirofiban might be associated with the restoration of eNOS/iNOS balance, since inhibition of NO production blocked the tirofiban-mediated renal protection on I/R injury. The present in vivo and in vitro study indicated that tirofiban pretreatment exerts a protective effect on I/R injury in kidney through regulation of eNOS/iNOS balance.
authors.affiliation: Department of Cardiology, Daping Hospital, The Third Military Medical University, 400042 Chongqing, China; Chongqing Institute of Cardiology, 400042 Chongqing, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Guan
authors.affiliation: Department of Cardiology, Daping Hospital, The Third Military Medical University, 400042 Chongqing, China; Chongqing Institute of Cardiology, 400042 Chongqing, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Daping Hospital, The Third Military Medical University, 400042 Chongqing, China; Chongqing Institute of Cardiology, 400042 Chongqing, China.
authors.forename: Yukai
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Cardiology, Daping Hospital, The Third Military Medical University, 400042 Chongqing, China; Chongqing Institute of Cardiology, 400042 Chongqing, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Han
authors.affiliation: Department of Cardiology, Daping Hospital, The Third Military Medical University, 400042 Chongqing, China; Chongqing Institute of Cardiology, 400042 Chongqing, China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Ren
authors.affiliation: Department of Cardiology, Daping Hospital, The Third Military Medical University, 400042 Chongqing, China; Chongqing Institute of Cardiology, 400042 Chongqing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Eric Wang
authors.affiliation: Department of Cardiology, Daping Hospital, The Third Military Medical University, 400042 Chongqing, China; Chongqing Institute of Cardiology, 400042 Chongqing, China. Electronic address: yangjianac@163.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Cardiology, Daping Hospital, The Third Military Medical University, 400042 Chongqing, China; Chongqing Institute of Cardiology, 400042 Chongqing, China. Electronic address: zhoulin@mail.tmmu.com.cn.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Cardiology, Daping Hospital, The Third Military Medical University, 400042 Chongqing, China; Chongqing Institute of Cardiology, 400042 Chongqing, China. Electronic address: cyzeng1@hotmail.com.
authors.forename: Chunyu
authors.initials: C
authors.lastname: Zeng
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D015415:Biomarkers / Q000097:blood; D001806:Blood Urea Nitrogen; D002460:Cell Line; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D003404:Creatinine / Q000097:blood; D019610:Cytoprotection; D004195:Disease Models, Animal; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D007674:Kidney Diseases / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D000077466:Tirofiban; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D014443:Tyrosine; D003404:Creatinine; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; C496318:Nos2 protein, rat; C496316:Nos3 protein, rat; D000077466:Tirofiban
keywords: Apoptosis; Oxidative stress; Renal ischemia–reperfusion; Tirofiban