title: A Mimivirus Enzyme that Participates in Viral Entry.
abstract: Mimivirus was initially identified as a bacterium because its dense, 125-nm-long fibers stained Gram-positively. These fibers probably play a role during the infection of some host cells. The normal hosts of Mimivirus are unknown, but in the laboratory Mimivirus is usually propagated in amoeba. The structure of R135, a major component of the fibrous outer layer of Mimivirus, has been determined to 2-Å resolution. The protein's structure is similar to that of members of the glucose-methanol-choline oxidoreductase family, which have an N-terminal FAD binding domain and a C-terminal substrate recognition domain. The closest homolog to R135 is an aryl-alcohol oxidase that participates in lignin biodegradation of plant cell walls. Thus R135 might participate in the degradation of their normal hosts, including some lignin-containing algae.
authors.affiliation: Department of Biological Sciences, Purdue University, 240 South Martin Jischke Drive, West Lafayette, IN 47907-2032, USA.
authors.forename: Thomas
authors.initials: T
authors.lastname: Klose
authors.affiliation: Department of Biological Sciences, Purdue University, 240 South Martin Jischke Drive, West Lafayette, IN 47907-2032, USA.
authors.forename: Dominik A
authors.initials: DA
authors.lastname: Herbst
authors.affiliation: Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: Hanyu
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: Joann P
authors.initials: JP
authors.lastname: Max
authors.affiliation: Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: Hilkka I
authors.initials: HI
authors.lastname: Kenttämaa
authors.affiliation: Department of Biological Sciences, Purdue University, 240 South Martin Jischke Drive, West Lafayette, IN 47907-2032, USA. Electronic address: mr@purdue.edu.
authors.forename: Michael G
authors.initials: MG
authors.lastname: Rossmann
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003461:Crystallography; D019281:Dimerization; D004591:Electrophoresis, Polyacrylamide Gel; D054884:Host-Pathogen Interactions; D013058:Mass Spectrometry; D056686:Mimiviridae / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular; D015394:Molecular Structure; D011485:Protein Binding; D011487:Protein Conformation; D013379:Substrate Specificity; D015678:Viral Structural Proteins / Q000737:chemistry*; D053586:Virus Internalization
chemical_list: D015678:Viral Structural Proteins