title: Metabolic profiling of antioxidants constituents in Artemisia selengensis leaves.
abstract: This study aimed to evaluate the antioxidant potential of Artemisia selengensis Turcz (AST) leaves, a byproduct when processing AST stalk, and identify the antioxidant constituents by using HPLC-QTOF-MS(2). The total phenolics content (TPC), total flavonoids content (TFC) and antioxidant abilities of fractions resulted from the successively partition of chloroform, ethyl acetate and n-butanol were compared. Ethyl acetate fraction (EAF) exhibited the highest TFC (65.44 mg QuE/g fraction), n-butanol fraction (nBuF) showed the highest TPC (384.78 mg GAE/g fraction) and the best DPPH scavenging ability, ABTS(+) scavenging ability and reducing power. Totally, 57 compounds were identified or tentatively identified in nBuF and EAF, 40 of them were reported in AST for the first time. The major constituents in EAF were flavonoids, and the major constituents in nBuF were phenolic acids and organic acids. Thus, AST leaves might be a potential low-cost resource of natural antioxidants.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; College of Life Science, Jiangxi Normal University, Nanchang 330022, China. Electronic address: tuzc_mail@aliyun.com.
authors.forename: Zong-cai
authors.initials: ZC
authors.lastname: Tu
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. Electronic address: wanghui00072@aliyun.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors.forename: Zhi-feng
authors.initials: ZF
authors.lastname: Fu
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors.forename: Qing-hui
authors.initials: QH
authors.lastname: Wen
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Fan
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000378:metabolism; D019306:Artemisia / Q000737:chemistry* / Q000378:metabolism; D005419:Flavonoids / Q000737:chemistry / Q000378:metabolism; D055432:Metabolomics; D010636:Phenols / Q000737:chemistry / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry* / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts
keywords: 3,5-Dicaffeoylquinic acid (PubChem CID: 6474310); 3-Caffeoylquinic acid (PubChem CID: 1794427); Antioxidant activity; Artemisia selengensis Turcz; Ferulic acid (PubChem CID: 445858); HPLC-QTOF-MS(2); Kaempferol-3-O-galactoside (PubChem CID: 5282149); Kaempferol-3-O-glucoside (PubChem CID: 5282102); Polyphenols; Protocatechualdehyde (PubChem CID: 8768); Quercetin-3-O-galactoside (PubChem CID: 5281643); Quercetin-3-O-glucoside (PubChem CID: 54758678); Rutin (PubChem CID: 5280805); Uridine (PubChem CID: 6029)