title: Inhibition of Azoxymethane-induced Colorectal Aberrant Crypt Foci in Mice Fed a High-fat Diet by Pleurotus eryngii (Eringi) and Hypsizygus marmoreus (Bunashimeji).
abstract: Obesity markedly increases the risk of colorectal cancer. Recently, the preventive effects of edible mushrooms on triglyceride elevation and visceral fat accumulation have been reported. Here, the effects of Pleurotus eryngii (Eringi) and Hypsizygus marmoreus (Bunashimeji) on azoxymethane (AOM)-induced aberrant crypt foci (ACF; precancerous lesions) in the colorectums of mice fed a high-fat diet were examined. Eringi (ER) and Bunashimeji (BU) mushroom powder samples were used. Six-week-old male C57BL/6J mice received an intraperitoneal injection of AOM (10 mg/kg) once a week for two weeks, and were sacrificed and dissected at 6 weeks after the start of the experiment. After the initiation of the experiment, they received a normal diet (ND), high-fat diet (HFD), HFD + ER (1 or 5% of diet), or HFD + BU (1 or 5% of diet). As a result, body and fat weights were significantly lower in the 5% ER and BU groups than in the HFD group. Liver triglyceride levels were also significantly lower in the 5% ER and BU groups. Total liver cholesterol levels were significantly lower in the 5% ER group. The numbers of ACF (especially large ACF) showed strong inhibitory effects in both ER and BU groups. Measurement of the cell proliferation marker Ki-67 labeling index in the colonic mucosa demonstrated more significant suppression in both ER and BU groups than in the HFD group. These results suggest that the simultaneous intake of ER and BU may inhibit colorectal tumorigenesis in HFD-fed mice.
authors.affiliation: Division of Biochemistry, Fujita Memorial Nanakuri Institute, Fujita Health University, Tsu, Mie, Japan E-mail : tchihara@fujita-hu.ac.jp.
authors.forename: Takaaki
authors.initials: T
authors.lastname: Kaneko
authors.forename: Takeshi
authors.initials: T
authors.lastname: Chihara
authors.forename: Kan
authors.initials: K
authors.lastname: Shimpo
authors.forename: Hidehiko
authors.initials: H
authors.lastname: Beppu
authors.forename: Takashi
authors.initials: T
authors.lastname: Higashiguchi
authors.forename: Shigeru
authors.initials: S
authors.lastname: Sonoda
mesh_terms: D058739:Aberrant Crypt Foci / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D001397:Azoxymethane / Q000633:toxicity*; D001835:Body Weight / Q000187:drug effects; D002273:Carcinogens / Q000633:toxicity; D002784:Cholesterol / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D059305:Diet, High-Fat / Q000009:adverse effects*; D007124:Immunoenzyme Techniques; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011208:Powders / Q000494:pharmacology*; D014280:Triglycerides / Q000378:metabolism
chemical_list: D002273:Carcinogens; D011208:Powders; D014280:Triglycerides; D002784:Cholesterol; D001397:Azoxymethane