title: UV-B stress induced metabolic rearrangements explored with comparative proteomics in three Anabaena species.
abstract: Comparative proteomics together with physiological variables revealed different responses among three species of diazotrophic cyanobacterium Anabaena exposed to UV-B stress at the same time points. Perceptible decline in PSII activity, ATP pool, nitrogenase activity and respiration rate was observed for all the three species; this being maximum in Anabaena doliolum, followed by Anabaena sp. PCC 7120 and minimum in Anabaena L31. Statistical analysis of the protein abundance divided majority of them as early accumulated in A. L31, late accumulated in A. sp. PCC 7120 and downregulated in A. doliolum. Tolerance of A. L31 may be ascribed to post-translational modification reflected through the highest number of protein isoforms in its proteome followed by A. PCC 7120 and A. doliolum. Furthermore, increase in abundance of cyanophycinase, glutamine synthetase and succinate semialdehyde dehydrogenase in A. L31 suggests operation of an alternate pathway for assimilation of nitrogen and carbon under UV-B stress. An early accumulation of four proteins viz., glutamate ammonia ligase (Alr2328), transketolase (Alr3344), inorganic pyrophosphatase (All3570), and trigger protein (Alr3681) involved respectively in amino acid metabolism, energy metabolism, biosynthesis of cofactor and trigger protein and chaperone like activity across three species, suggests them to be marker of UV-B stress in Anabaena spp. This article is part of a Special Issue entitled: Proteomics in India.
authors.affiliation: Molecular Biology Section, Center of Advanced Study in Botany, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Alok Kumar
authors.initials: AK
authors.lastname: Shrivastava
authors.affiliation: Molecular Biology Section, Center of Advanced Study in Botany, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Antra
authors.initials: A
authors.lastname: Chatterjee
authors.affiliation: Molecular Biology Section, Center of Advanced Study in Botany, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Shivam
authors.initials: S
authors.lastname: Yadav
authors.affiliation: Molecular Biology Section, Center of Advanced Study in Botany, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Prashant Kumar
authors.initials: PK
authors.lastname: Singh
authors.affiliation: Molecular Biology Section, Center of Advanced Study in Botany, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Shilpi
authors.initials: S
authors.lastname: Singh
authors.affiliation: Molecular Biology Section, Center of Advanced Study in Botany, Banaras Hindu University, Varanasi 221005, India.. Electronic address: lcrbhu15@gmail.com.
authors.forename: L C
authors.initials: LC
authors.lastname: Rai
mesh_terms: D017033:Anabaena / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D040901:Proteomics; D013312:Stress, Physiological / Q000528:radiation effects*; D014466:Ultraviolet Rays
chemical_list: D001426:Bacterial Proteins
keywords: 2D-electrophoresis; AHC; Anabaena; MALDI-TOFMS/MS; PCA; UV-B