title: Loop-Mediated Isothermal Amplification (LAMP) for Detection of Phytoplasmas in the Field.
abstract: Loop-mediated isothermal amplification (LAMP) is a nucleic acid-based detection method with many applications. This chapter details its use for detection of phytoplasma diseases, combining a rapid 2-min DNA extraction method with real-time LAMP product detection such that the entire procedure can be undertaken and completed in the field within 40 min. Furthermore, the assays include an anneal curve validation step to guard against false positives and an assay for host plant DNA to guard against false negatives.
authors.affiliation: School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK, matthew.dickinson@nottingham.ac.uk.
authors.forename: Matt
authors.initials: M
authors.lastname: Dickinson
mesh_terms: D030008:Catharanthus / Q000235:genetics / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D018744:DNA, Plant / Q000032:analysis* / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D045582:Phytoplasma / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000235:genetics / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D018744:DNA, Plant