title: Biopolymers for sample collection, protection, and preservation.
abstract: One of the principal challenges in the collection of biological samples from air, water, and soil matrices is that the target agents are not stable enough to be transferred from the collection point to the laboratory of choice without experiencing significant degradation and loss of viability. At present, there is no method to transport biological samples over considerable distances safely, efficiently, and cost-effectively without the use of ice or refrigeration. Current techniques of protection and preservation of biological materials have serious drawbacks. Many known techniques of preservation cause structural damages, so that biological materials lose their structural integrity and viability. We review applications of a novel bacterial preservation process, which is nontoxic and water soluble and allows for the storage of samples without refrigeration. The method is capable of protecting the biological sample from the effects of environment for extended periods of time and then allows for the easy release of these collected biological materials from the protective medium without structural or DNA damage. Strategies for sample collection, preservation, and shipment of bacterial, viral samples are described. The water-soluble polymer is used to immobilize the biological material by replacing the water molecules within the sample with molecules of the biopolymer. The cured polymer results in a solid protective film that is stable to many organic solvents, but quickly removed by the application of the water-based solution. The process of immobilization does not require the use of any additives, accelerators, or plastifiers and does not involve high temperature or radiation to promote polymerization.
authors.affiliation: Department of Anatomy, Physiology and Pharmacology, Auburn, AL, 36849, USA.
authors.forename: Iryna
authors.initials: I
authors.lastname: Sorokulova
authors.forename: Eric
authors.initials: E
authors.lastname: Olsen
authors.forename: Vitaly
authors.initials: V
authors.lastname: Vodyanoy
mesh_terms: D001419:Bacteria / Q000302:isolation & purification*; D001704:Biopolymers / Q000378:metabolism*; D008828:Microbiological Techniques / Q000379:methods*; D011309:Preservation, Biological / Q000379:methods*; D013048:Specimen Handling / Q000379:methods*; D014780:Viruses / Q000302:isolation & purification*
chemical_list: D001704:Biopolymers