title: [Establishment a method for identification of the poly(ADP-ribose) binding proteins induced by benzo(a)pyrene].
abstract: OBJECTIVE
  To establish a method for identification of the poly(ADP-ribose) binding proteins induced by benzo (a) pyrene.
  
  
  METHODS
  Poly (ADP-ribose) binding protein were screened by immunoprecipitation assay and further separated by high performance liquid chromatography (HPLC) and two dimensional electrophoresis, then identified by MALDI-TOF-MS/MS. The proteins sequence were identified by two methods and compared the common binding motif with literature reports.
  
  
  RESULTS
  Three poly (ADP-ribose) binding proteins were identified by MALDI-TOF-MS/MS combined with immunoprecipitation assay and HPLC, and twelve poly (ADP-ribose) binding proteins were identified by MALDI-TOF-MS/MS combined with immunoprecipitation assay and two dimensional electrophoresis. Most of them have a common binding motif which was consistent with the reported.
  
  
  CONCLUSIONS
  Combined the immunoprecipitation assay and two dimensional electrophoresis with MALDI-TOF-MS/MS could be used to analyze the poly(ADP-ribose) binding proteins, and these proteins have a common conserved binding motif.
authors.forename: Wei
authors.initials: W
authors.lastname: Gao
authors.forename: Haiyan
authors.initials: H
authors.lastname: Huang
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Cai
authors.forename: Xuan
authors.initials: X
authors.lastname: Li
authors.forename: Yinpin
authors.initials: Y
authors.lastname: Liu
authors.forename: Jianjun
authors.initials: J
authors.lastname: Liu
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: Zhuang
authors.forename: Wen
authors.initials: W
authors.lastname: Chen
mesh_terms: D001564:Benzo(a)pyrene; D011064:Poly Adenosine Diphosphate Ribose / Q000378:metabolism*; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: D011506:Proteins; D011064:Poly Adenosine Diphosphate Ribose; D001564:Benzo(a)pyrene