title: Risungbinella pyongyangensis gen. nov., sp. nov., a mesophilic member of the family Thermoactinomycetaceae isolated from an agricultural soil sample.
abstract: A mesophilic strain, designed MC 210T, was isolated from an agricultural soil sample from Pyongyang, Democratic People's Republic of Korea, and its taxonomic position was investigated by using a polyphasic approach. The novel strain grew well on PYI medium, and no diffusible pigments were produced. The optimum temperature for growth was 37 °C. The aerial mycelium was well developed, but not fragmented. The strain was Gram-reaction-positive and non-motile and formed endospores on the aerial mycelium. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain MC 210T belongs to the family Thermoactinomycetaceae. Strain MC 210T showed 16S rRNA gene sequence similarities of 92.90 and 92.54% to the type strains of Geothermomicrobium terrae and Shimazuella kribbensis, respectively. The cell wall of strain MC 210T contained meso-diaminopimelic acid, glutamic acid and alanine as the diagnostic amino acids, and whole-cell hydrolysates contained glucose, arabinose and galactose. Strain MC 210T contained anteiso-C13 : 0, iso-C14 : 0, C14 : 0, anteiso-C15 : 0, C16 : 0 and iso-C13 : 0 as the major cellular fatty acids. The main polar lipids were phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, two unknown aminophospholipids, an unknown aminolipid, three unknown phospholipids and five unknown polar lipids. The predominant menaquinone was MK-7.The G+C content of the genomic DNA was 42.1 mol%. On the basis of our phylogenetic, physiological and chemotaxonomic data, strain MC 210T is considered to represent a novel genus and species, for which we propose the name Risungbinella pyongyangensis gen. nov., sp. nov., in the family Thermoactinomycetaceae. The type strain of Risungbinella pyongyangensis is MC 210T (CCTCC AA 2013021T = NRRL B-59118T).
authors.affiliation: China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.|College of Life Sciences, Kim Il Sung University, Pyongyang, Democratic People's Republic of Korea.
authors.forename: MyongChol
authors.initials: M
authors.lastname: Kim
authors.affiliation: China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.|College of Life Sciences, Kim Il Sung University, Pyongyang, Democratic People's Republic of Korea.
authors.forename: TongRyul
authors.initials: T
authors.lastname: Kim
authors.affiliation: China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.|College of Life Sciences, Kim Il Sung University, Pyongyang, Democratic People's Republic of Korea.
authors.forename: SongYong
authors.initials: S
authors.lastname: Ri
authors.affiliation: China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Fan
authors.initials: F
authors.lastname: Jiang
authors.affiliation: China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Xulu
authors.initials: X
authors.lastname: Chang
authors.affiliation: China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Lvzhi
authors.initials: L
authors.lastname: Ren
authors.affiliation: China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Qiu
authors.affiliation: China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Sangsang
authors.initials: S
authors.lastname: Deng
authors.affiliation: China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Chengxiang
authors.initials: C
authors.lastname: Fang
authors.affiliation: China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Congyi
authors.initials: C
authors.lastname: Zheng
authors.affiliation: China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Peng
mesh_terms: D056449:Bacillales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid; C062629:menaquinone 7