title: Precise Genome Editing of Drosophila with CRISPR RNA-Guided Cas9.
abstract: The readily programmable CRISPR-Cas9 system is transforming genome engineering. We and others have adapted the S. pyogenes CRISPR-Cas9 system to precisely engineer the Drosophila genome and demonstrated that these modifications are efficiently transmitted through the germline. Here we provide a detailed protocol for engineering small indels, defined deletions, and targeted insertion of exogenous DNA sequences within one month using a rapid DNA injection-based approach.
authors.affiliation: Genetics Training Program, University of Wisconsin-Madison, Madison, WI, 53706, USA.
authors.forename: Scott J
authors.initials: SJ
authors.lastname: Gratz
authors.forename: Melissa M
authors.initials: MM
authors.lastname: Harrison
authors.forename: Jill
authors.initials: J
authors.lastname: Wildonger
authors.forename: Kate M
authors.initials: KM
authors.lastname: O'Connor-Giles
mesh_terms: D000818:Animals; D064130:CRISPR-Associated Proteins / Q000378:metabolism*; D064113:CRISPR-Cas Systems / Q000235:genetics*; D004260:DNA Repair; D004330:Drosophila / Q000196:embryology / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000378:metabolism; D018390:Gene Targeting; D005818:Genetic Engineering / Q000379:methods*; D016678:Genome / Q000235:genetics*; D059765:Homologous Recombination; D010957:Plasmids / Q000235:genetics; D012313:RNA / Q000235:genetics*
chemical_list: D064130:CRISPR-Associated Proteins; D012313:RNA