title: Expression and Anthocyanin Biosynthesis-Modulating Potential of Sweet Cherry (Prunus avium L.) MYB10 and bHLH Genes.
abstract: Anthocyanins are essential contributors to fruit coloration, an important quality feature and a breed determining trait of a sweet cherry fruit. It is well established that the biosynthesis of anthocyanins is regulated by an interplay of specific transcription factors belonging to MYB and bHLH families accompanied by a WD40 protein. In this study, we isolated and analyzed PaWD40, PabHLH3, PabHLH33, and several closely related MYB10 gene variants from different cultivars of sweet cherry, analyzed their expression in fruits with different anthocyanin levels at several developmental stages, and determined their capabilities to modulate anthocyanin synthesis in leaves of two Nicotiana species. Our results indicate that transcription level of variant PaMYB10.1-1 correlates with fruit coloration, but anthocyanin synthesis in Nicotiana was induced by another variant, PaMYB10.1-3, which is moderately expressed in fruits. The analysis of two fruit-expressed bHLH genes revealed that PabHLH3 enhances MYB-induced anthocyanin synthesis, whereas PabHLH33 has strong inhibitory properties.
authors.affiliation: Vilnius University Institute of Biotechnology, V.A. Graičiūno 8, Vilnius, LT-02241, Lithuania.
authors.forename: Pavel
authors.initials: P
authors.lastname: Starkevič
authors.affiliation: Vilnius University Institute of Biotechnology, V.A. Graičiūno 8, Vilnius, LT-02241, Lithuania.
authors.forename: Jurgita
authors.initials: J
authors.lastname: Paukštytė
authors.affiliation: Vilnius University Institute of Biotechnology, V.A. Graičiūno 8, Vilnius, LT-02241, Lithuania.
authors.forename: Vaiva
authors.initials: V
authors.lastname: Kazanavičiūtė
authors.affiliation: Vilnius University Institute of Biotechnology, V.A. Graičiūno 8, Vilnius, LT-02241, Lithuania.
authors.forename: Erna
authors.initials: E
authors.lastname: Denkovskienė
authors.affiliation: Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Kaunas st 30, Babtai, LT-54333, Kaunas, Lithuania.
authors.forename: Vidmantas
authors.initials: V
authors.lastname: Stanys
authors.affiliation: Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Kaunas st 30, Babtai, LT-54333, Kaunas, Lithuania.
authors.forename: Vidmantas
authors.initials: V
authors.lastname: Bendokas
authors.affiliation: Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Kaunas st 30, Babtai, LT-54333, Kaunas, Lithuania.
authors.forename: Tadeušas
authors.initials: T
authors.lastname: Šikšnianas
authors.affiliation: Vilnius University Institute of Biotechnology, V.A. Graičiūno 8, Vilnius, LT-02241, Lithuania.
authors.forename: Aušra
authors.initials: A
authors.lastname: Ražanskienė
authors.affiliation: Vilnius University Institute of Biotechnology, V.A. Graičiūno 8, Vilnius, LT-02241, Lithuania.
authors.forename: Raimundas
authors.initials: R
authors.lastname: Ražanskas
mesh_terms: D000872:Anthocyanins / Q000096:biosynthesis* / Q000235:genetics; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics* / Q000502:physiology; D003001:Cloning, Molecular; D005638:Fruit / Q000378:metabolism; D020869:Gene Expression Profiling; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000502:physiology; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D000068220:Prunus avium / Q000235:genetics* / Q000378:metabolism; D014026:Nicotiana / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000502:physiology
chemical_list: D000872:Anthocyanins; D051792:Basic Helix-Loop-Helix Proteins; D010940:Plant Proteins; D014157:Transcription Factors