title: Phytochemical analysis and antioxidant potential of the leaves of Garcinia travancorica Bedd.
abstract: Phytochemical analysis of the leaves of Garcinia travancorica, a hitherto uninvestigated endemic species to the Western Ghats of south India, resulted in isolation and characterisation of the polyisoprenylated benzophenones 7-epi-nemorosone (1) and garcinol (2) along with biflavonoids GB-1a (3), GB-1 (4), GB-2 (5), morelloflavone (6) and morelloflavone-7″-O-β-D-glycoside or fukugiside (7). The compounds were identified using various spectroscopic techniques, mainly through NMR and MS. The methanol extract and the biflavonoids 3, 4, 5 and 7 showed potential in vitro antioxidant activities. The IC50 value of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of compound 7 was 8.34 ± 2.12 μg/mL, comparable to that of standard ascorbic acid (3.2 ± 0.50 μg/mL). In the superoxide radical scavenging assay, compound 7 gave IC50 value of 6.95 ± 1.33 μg/mL close to standard ascorbic acid with IC50 value of 5.8 ± 0.25 μg/mL. Validated HPTLC estimation revealed G. travancorica as a rich source of morelloflavone-7″-O-β-D-glycoside (7.12% dry wt. leaves).
authors.affiliation: a Phytochemistry and Phytopharmacology Division, Jawaharlal Nehru Tropical Botanic Garden and Research Institute, Palode , Thiruvananthapuram 695562 , Kerala , India.
authors.forename: A P Anu
authors.initials: AP
authors.lastname: Aravind
authors.affiliation: b Govt. Arts College , Paramakudy , Tamilnadu , India.
authors.forename: K R T
authors.initials: KR
authors.lastname: Asha
authors.affiliation: a Phytochemistry and Phytopharmacology Division, Jawaharlal Nehru Tropical Botanic Garden and Research Institute, Palode , Thiruvananthapuram 695562 , Kerala , India.
authors.forename: K B
authors.initials: KB
authors.lastname: Rameshkumar
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000737:chemistry / Q000494:pharmacology; D001577:Benzophenones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D044946:Biflavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D001713:Biphenyl Compounds; D004353:Drug Evaluation, Preclinical / Q000379:methods; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D029760:Garcinia / Q000737:chemistry*; D006027:Glycosides; D007194:India; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D064209:Phytochemicals / Q000032:analysis / Q000737:chemistry; D010851:Picrates; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry*; D013729:Terpenes / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D001577:Benzophenones; D044946:Biflavonoids; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D006027:Glycosides; D064209:Phytochemicals; D010851:Picrates; D010936:Plant Extracts; D013729:Terpenes; C000609513:morelloflavone-7'-O-beta-D-glycoside; C437463:nemorosone; C105620:morelloflavone; C004931:1,1-diphenyl-2-picrylhydrazyl; D001205:Ascorbic Acid; C054597:garcinol
keywords: Garcinia travancorica; HPTLC; antioxidant; fukugiside; garcinol