title: Novel HPLC--Fluorescence methodology for the determination of methylglyoxal and glyoxal. Application to the analysis of monovarietal wines "Ribera del Guadiana".
abstract: The determination and quantification of α-dicarbonyl compounds, glyoxal and methylglyoxal, in "Ribera del Guadiana" monovarietal wines (Extremadura, Spain) without sample clean-up has been carried out by HPLC with spectrofluorimetric detection (307/371 nm). For this purpose, a derivatization step with the new reagent 3,4-diaminopyridine at pH 2 during 120 min at 90 °C has been included. Afterwards, the sample could be injected in the chromatographic system with no clean-up, during a total run time of 4 min. Several monovarietal wines (white, rosé and red) have been analyzed and the levels of these compounds for white wines were between 0.4-1.0 mg L(-1) glyoxal and 0.8-1.3 mg L(-1) methylglyoxal; and between 0.8-3.0 mg L(-1) and 0.5-1.8 mg L(-1) of glyoxal and methylglyoxal respectively, in red wines.
authors.affiliation: Department of Analytical Chemistry and IACYS, University of Extremadura, 06006 Badajoz, Spain. Electronic address: maribelro@unex.es.
authors.forename: Maria Isabel
authors.initials: MI
authors.lastname: Rodríguez-Cáceres
authors.affiliation: Department of Analytical Chemistry and IACYS, University of Extremadura, 06006 Badajoz, Spain.
authors.forename: Mónica
authors.initials: M
authors.lastname: Palomino-Vasco
authors.affiliation: Department of Analytical Chemistry and IACYS, University of Extremadura, 06006 Badajoz, Spain.
authors.forename: Nielene
authors.initials: N
authors.lastname: Mora-Diez
authors.affiliation: Department of Analytical Chemistry and IACYS, University of Extremadura, 06006 Badajoz, Spain.
authors.forename: Maria Isabel
authors.initials: MI
authors.lastname: Acedo-Valenzuela
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005453:Fluorescence; D006037:Glyoxal / Q000032:analysis*; D011765:Pyruvaldehyde / Q000032:analysis*; D013030:Spain; D014920:Wine / Q000032:analysis*
chemical_list: D006037:Glyoxal; D011765:Pyruvaldehyde
keywords: 2,3-Diaminopyridine (PubChem CID: 9956); 3,4-Diaminopyridine (PubChem CID: 5918); Derivatization; Glyoxal; Glyoxal (PubChem CID: 7860); HPLC-fluorescence; Methylglyoxal; Methylglyoxal (PubChem CID: 880); Monovarietal wines; Ribera del Guadiana