title: DNA replication origin activation in space and time.
abstract: DNA replication begins with the assembly of pre-replication complexes (pre-RCs) at thousands of DNA replication origins during the G1 phase of the cell cycle. At the G1-S-phase transition, pre-RCs are converted into pre-initiation complexes, in which the replicative helicase is activated, leading to DNA unwinding and initiation of DNA synthesis. However, only a subset of origins are activated during any S phase. Recent insights into the mechanisms underlying this choice reveal how flexibility in origin usage and temporal activation are linked to chromosome structure and organization, cell growth and differentiation, and replication stress.
authors.affiliation: Institute of Human Genetics, CNRS, 141 rue de la Cardonille, 34396 Montpellier, France.
authors.forename: Michalis
authors.initials: M
authors.lastname: Fragkos
authors.affiliation: 1] Institute of Human Genetics, CNRS, 141 rue de la Cardonille, 34396 Montpellier, France. [2].
authors.forename: Olivier
authors.initials: O
authors.lastname: Ganier
authors.affiliation: 1] Institute of Human Genetics, CNRS, 141 rue de la Cardonille, 34396 Montpellier, France. [2].
authors.forename: Philippe
authors.initials: P
authors.lastname: Coulombe
authors.affiliation: Institute of Human Genetics, CNRS, 141 rue de la Cardonille, 34396 Montpellier, France.
authors.forename: Marcel
authors.initials: M
authors.lastname: Méchali
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002877:Chromosomes, Human / Q000235:genetics / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis* / Q000235:genetics; D004261:DNA Replication / Q000502:physiology*; D016193:G1 Phase / Q000502:physiology*; D006801:Humans; D018741:Replication Origin / Q000502:physiology*; D016196:S Phase / Q000502:physiology*
chemical_list: D004247:DNA