title: Use of the amniotic membrane to cover the peritoneal cavity in the reconstruction of the abdominal wall with polypropylene mesh in rats.
abstract: OBJECTIVE
  to evaluate the efficacy of the amniotic membrane used with polypropylene mesh against the formation of adhesions and its influence on healing.
  
  
  METHODS
  twenty five female Wistar rats were anesthetized for creating a parietal defect in the anterior abdominal wall. Its correction was made with polypropylene mesh alone and associated with amniotic membrane. In the control group (n=11), the screen was inserted alone. In group A (n=7) we interposed the amniotic membrane between the screen and the abdominal wall. In group B, the amniotic membrane was placed on the mesh, covering it. After seven days, the animals were euthanized for macroscopic and microscopic evaluation of healing.
  
  
  RESULTS
  adhesions were observed in all animals except one in the control group. Severe inflammation was observed in all animals in groups A and B and in three of the control group, with significant difference between them (A and B with p=0.01). Pronounced angiogenic activity was noted in one animal in the control group, six in group A and four in group B, with a significant difference between the control group and group A (p=0.002) and group B (p=0.05). The scar collagen was predominantly mature, except in five animals of the control group, with significant difference between the control group and group A (p=0.05) and group B (p=0.05).
  
  
  CONCLUSIONS
  The amniotic membrane did not alter the formation of adhesions in the first postoperative week. There were also pronounced inflammation, high angiogenic activity and predominance of mature collagen fibers, regardless of the anatomical plane that it was inserted in.
authors.affiliation: Department of Surgery, Faculty of Medicine, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Rafael Calvão
authors.initials: RC
authors.lastname: Barbuto
authors.affiliation: Department of Surgery, Faculty of Medicine, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Ivana Duval
authors.initials: ID
authors.lastname: de Araujo
authors.affiliation: Instituto Mário Penna, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Daniel de Oliveira
authors.initials: Dde O
authors.lastname: Bonomi
authors.affiliation: Faculty of Medical Sciences, Universidade José do Rosário Vellano, Belo Horizonte, Minas Gerais State, Brazil.
authors.forename: Luciene Simões de Assis
authors.initials: LS
authors.lastname: Tafuri
authors.affiliation: Faculty of Medical Sciences, Universidade José do Rosário Vellano, Belo Horizonte, Minas Gerais State, Brazil.
authors.forename: Antônio
authors.initials: A
authors.lastname: Calvão Neto
authors.affiliation: Faculty of Medical Sciences, Universidade José do Rosário Vellano, Belo Horizonte, Minas Gerais State, Brazil.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Malinowski
authors.affiliation: Faculty of Medical Sciences, Universidade José do Rosário Vellano, Belo Horizonte, Minas Gerais State, Brazil.
authors.forename: Vinícius Silveira dos Santos
authors.initials: VS
authors.lastname: Bardin
authors.affiliation: Faculty of Medicine, Centro Universitário de Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Mateus Duarte
authors.initials: MD
authors.lastname: Leite
authors.affiliation: STK Núcleo de Cirurgia Plástica, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Ian Göedert Leite
authors.initials: IG
authors.lastname: Duarte
mesh_terms: D034861:Abdominal Wall / Q000601:surgery*; D058107:Abdominal Wound Closure Techniques; D000650:Amnion; D000818:Animals; D005260:Female; D010529:Peritoneal Cavity / Q000601:surgery*; D011126:Polypropylenes; D051381:Rats; D017208:Rats, Wistar; D013526:Surgical Mesh; D000267:Tissue Adhesions / Q000517:prevention & control*; D014945:Wound Healing
chemical_list: D011126:Polypropylenes