title: Divergent signaling pathways regulate IL-12 production induced by different species of Lactobacilli in human dendritic cells.
abstract: Recent studies have indicated that different strains of Lactobacilli differ in their ability to regulate IL-12 production by dendritic cells (DCs), as some strains are stronger inducer of IL-12 while other are not and can even inhibit IL-12 production stimulated by IL-12-inducer Lactobacilli. In this report we demonstrate that Lactobacillus reuteri 5289, as previously described for other strains of L. reuteri, can inhibit DC production of IL-12 induced by Lactobacilllus acidophilus NCFM. Remarkably, L. reuteri 5289 was able to inhibit IL-12 production induced not only by Lactobacilli, as so far reported, but also by bacteria of different genera, including pathogens. We investigated in human DCs the signal transduction pathways involved in the inhibition of IL-12 production induced by L. reuteri 5289, showing that this potential anti-inflammatory activity, which is also accompanied by an elevated IL-10 production, is associated to a prolonged phosphorilation of ERK1/2 MAP kinase pathway. Improved understanding of the immune regulatory mechanisms exerted by Lactobacilli is crucial for a more precise employment of these commensal bacteria as probiotics in human immune-mediated pathologies, such as allergies or inflammatory bowel diseases.
authors.affiliation: Laboratory of Immunology and Biotherapy, Dept. Human Pathology, University of Messina, Messina, Italy; Laboratory of Bioconversion Engineering and Microbiological Food Safety, Department of Biology, University of Mascara, Mascara, Algeria.
authors.forename: Yacine
authors.initials: Y
authors.lastname: Amar
authors.affiliation: Laboratory of Immunology and Biotherapy, Dept. Human Pathology, University of Messina, Messina, Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Rizzello
authors.affiliation: Laboratory of Immunology and Biotherapy, Dept. Human Pathology, University of Messina, Messina, Italy; Cell Therapy Program, University Hospital-A.O.U. Policlinico G.Martino, Messina, Italy.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Cavaliere
authors.affiliation: Laboratory of Immunology and Biotherapy, Dept. Human Pathology, University of Messina, Messina, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Campana
authors.affiliation: Laboratory of Immunology and Biotherapy, Dept. Human Pathology, University of Messina, Messina, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: De Pasquale
authors.affiliation: Laboratory of Immunology and Biotherapy, Dept. Human Pathology, University of Messina, Messina, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Barberi
authors.affiliation: Laboratory of Immunology and Biotherapy, Dept. Human Pathology, University of Messina, Messina, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Oliveri
authors.affiliation: Laboratory of Immunology and Biotherapy, Dept. Human Pathology, University of Messina, Messina, Italy.
authors.forename: Gaetana
authors.initials: G
authors.lastname: Pezzino
authors.affiliation: Laboratory of Immunology and Biotherapy, Dept. Human Pathology, University of Messina, Messina, Italy; Cell Therapy Program, University Hospital-A.O.U. Policlinico G.Martino, Messina, Italy.
authors.forename: Gregorio
authors.initials: G
authors.lastname: Costa
authors.affiliation: Laboratory of Bioconversion Engineering and Microbiological Food Safety, Department of Biology, University of Mascara, Mascara, Algeria.
authors.forename: Aicha Tirtouil
authors.initials: AT
authors.lastname: Meddah
authors.affiliation: Laboratory of Immunology and Biotherapy, Dept. Human Pathology, University of Messina, Messina, Italy; Cell Therapy Program, University Hospital-A.O.U. Policlinico G.Martino, Messina, Italy. Electronic address: guido.ferlazzo@unime.it.
authors.forename: Guido
authors.initials: G
authors.lastname: Ferlazzo
authors.affiliation: Laboratory of Immunology and Biotherapy, Dept. Human Pathology, University of Messina, Messina, Italy; Cell Therapy Program, University Hospital-A.O.U. Policlinico G.Martino, Messina, Italy.
authors.forename: Irene
authors.initials: I
authors.lastname: Bonaccorsi
mesh_terms: D000893:Anti-Inflammatory Agents / Q000276:immunology; D015703:Antigens, CD / Q000096:biosynthesis; D018122:B7-1 Antigen / Q000096:biosynthesis; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology*; D004926:Escherichia coli / Q000276:immunology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis; D007249:Inflammation / Q000276:immunology; D018664:Interleukin-12 / Q000096:biosynthesis / Q000276:immunology*; D007779:Lactobacillus acidophilus / Q000276:immunology*; D052200:Limosilactobacillus reuteri / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D011550:Pseudomonas aeruginosa / Q000276:immunology; D015398:Signal Transduction / Q000276:immunology*; D013211:Staphylococcus aureus / Q000276:immunology; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism; D000097802:CD83 Antigen
chemical_list: D000893:Anti-Inflammatory Agents; D015703:Antigens, CD; D018122:B7-1 Antigen; D048049:Extracellular Signal-Regulated MAP Kinases; D007136:Immunoglobulins; D018664:Interleukin-12; D008562:Membrane Glycoproteins; D048051:p38 Mitogen-Activated Protein Kinases; D000097802:CD83 Antigen
keywords: Dendritic cells; IL-12; Lactobacillus reuteri; MAP kinases; Probiotics