title: Effect of the treatment by slightly acidic electrolyzed water on the accumulation of γ-aminobutyric acid in germinated brown millet.
abstract: The accumulation of γ-aminobutyric acid and the microbial decontamination are concerned increasingly in the production of sprouts. In this work, the effect of the treatment by slightly acidic electrolyzed water on the accumulation of γ-aminobutyric acid in the germinated brown millet was evaluated by high performance liquid chromatography during germination. The results showed that slightly acidic electrolyzed water with appropriate available chlorine (15 or 30 mg/L) could promote the accumulation of γ-aminobutyric acid by up to 21% (P < 0.05). However, the treatment with slightly acidic electrolyzed water could not enhance the sprouts growth of the germinated brown millet. The catalase and peroxidase activities of the germinated brown millet during germination were in agreement with the sprouts growth. Our results suggested that the accumulation of γ-aminobutyric acid was independent of the length of sprouts in germinated grains. Moreover, the treatment with slightly acidic electrolyzed water significantly reduced the microbial counts in the germinated millet (P < 0.05) and the treatment with high available chlorine concentration (15 and 30 mg/L) showed stronger anti-infection potential in the germinated brown millet than that of lower available chlorine concentration (5 mg/L). In conclusion, the treatment with slightly acidic electrolyzed water is an available approach to improve the accumulation of γ-aminobutyric acid and anti-infection potential in the germinated brown millet, and it can avoid too long millet sprouts.
authors.affiliation: Research Center for Fermentation Engineering of Hebei, College of Bioscience and Bioengineering, Hebei University of Science and Technology, No. 70 Yuhuadonglu, Shijiazhuang, Hebei 050018, PR China.
authors.forename: Xingfeng
authors.initials: X
authors.lastname: Li
authors.affiliation: Research Center for Fermentation Engineering of Hebei, College of Bioscience and Bioengineering, Hebei University of Science and Technology, No. 70 Yuhuadonglu, Shijiazhuang, Hebei 050018, PR China.
authors.forename: Jianxiong
authors.initials: J
authors.lastname: Hao
authors.affiliation: Research Center for Fermentation Engineering of Hebei, College of Bioscience and Bioengineering, Hebei University of Science and Technology, No. 70 Yuhuadonglu, Shijiazhuang, Hebei 050018, PR China.
authors.forename: Xianggui
authors.initials: X
authors.lastname: Liu
authors.affiliation: College of Food Science and Nutritional Engineering, China Agricultural University, P. O. Box 40, No. 17 Qinghuadonglu, Haidian, Beijing 100083, PR China.
authors.forename: Haijie
authors.initials: H
authors.lastname: Liu
authors.affiliation: Research Center for Fermentation Engineering of Hebei, College of Bioscience and Bioengineering, Hebei University of Science and Technology, No. 70 Yuhuadonglu, Shijiazhuang, Hebei 050018, PR China.
authors.forename: Yawei
authors.initials: Y
authors.lastname: Ning
authors.affiliation: Institute of Millet Crops, Hebei Academy of Agricultural and Forestry Sciences, 162 Hengshan Street, Shijiazhuang, Hebei 050035, PR China.
authors.forename: Ruhong
authors.initials: R
authors.lastname: Cheng
authors.affiliation: Academy of State Administration of Grain, No. 11 Baiwanzhuang Street, Beijing 100037, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Tan
authors.affiliation: Research Center for Fermentation Engineering of Hebei, College of Bioscience and Bioengineering, Hebei University of Science and Technology, No. 70 Yuhuadonglu, Shijiazhuang, Hebei 050018, PR China. Electronic address: foodsafetylab@163.com.
authors.forename: Yingmin
authors.initials: Y
authors.lastname: Jia
mesh_terms: D000890:Anti-Infective Agents; D002713:Chlorine / Q000008:administration & dosage; D004572:Electrolysis; D018525:Germination; D006863:Hydrogen-Ion Concentration; D012639:Seeds / Q000737:chemistry* / Q000254:growth & development* / Q000382:microbiology; D032983:Setaria Plant; D014867:Water / Q000737:chemistry*; D005680:gamma-Aminobutyric Acid / Q000032:analysis*
chemical_list: D000890:Anti-Infective Agents; D014867:Water; D002713:Chlorine; D005680:gamma-Aminobutyric Acid
keywords: Disinfection; Electrolyzed water; Germination; Millet; γ-Aminobutyric acid