title: Influence of the forms and levels of dietary selenium on antioxidant status and oxidative stress-related parameters in rainbow trout (Oncorhynchus mykiss) fry.
abstract: Se is an essential micronutrient required for normal growth, development and antioxidant defence. The objective of the present study was to assess the impact of dietary Se sources and levels on the antioxidant status of rainbow trout (Oncorhynchus mykiss) fry. First-feeding fry (initial body weight: 91 mg) were fed either a plant- or fishmeal-based diet containing 0·5 or 1·2 mg Se/kg diet supplemented or not with 0·3 mg Se/kg diet supplied as Se-enriched yeast or sodium selenite for 12 weeks at 17°C. Growth and survival of rainbow trout fry were not significantly affected by dietary Se sources and levels. Whole-body Se was raised by both Se sources and to a greater extent by Se-yeast. The reduced:oxidised glutathione ratio was raised by Se-yeast, whereas other lipid peroxidation markers were not affected by dietary Se. Whole-body Se-dependent glutathione peroxidase (GPX) activity was enhanced in fish fed Se-yeast compared to fish fed sodium selenite or non-supplemented diets. Activity and gene expression of this enzyme as well as gene expression of selenoprotein P (SelP) were reduced in fish fed the non-supplemented plant-based diet. Catalase, glutamate-cysteine ligase and nuclear factor-erythroid 2-related factor 2 (Nrf2) gene expressions were reduced by Se-yeast. These results suggest the necessity to supplement plant-based diets with Se for rainbow trout fry, and highlight the superiority of organic form of Se to fulfil the dietary Se requirement and sustain the antioxidant status of fish. GPX and SelP expression proved to be good markers of Se status in fish.
authors.affiliation: INRA, UR1067 NuMéA,F-64310St-Pée-sur-Nivelle,France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Fontagné-Dicharry
authors.affiliation: LCABIE UMR5254,F-64053Pau Cedex 09,France.
authors.forename: Simon
authors.initials: S
authors.lastname: Godin
authors.affiliation: INRA, UR1067 NuMéA,F-64310St-Pée-sur-Nivelle,France.
authors.forename: Haokun
authors.initials: H
authors.lastname: Liu
authors.affiliation: INRA, UR1067 NuMéA,F-64310St-Pée-sur-Nivelle,France.
authors.forename: Philip
authors.initials: P
authors.lastname: Antony Jesu Prabhu
authors.affiliation: LCABIE UMR5254,F-64053Pau Cedex 09,France.
authors.forename: Brice
authors.initials: B
authors.lastname: Bouyssière
authors.affiliation: LCABIE UMR5254,F-64053Pau Cedex 09,France.
authors.forename: Maïté
authors.initials: M
authors.lastname: Bueno
authors.affiliation: Lesaffre Feed Additives,F-59700Marcq-en-Barœul,France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Tacon
authors.affiliation: INRA, UR1067 NuMéA,F-64310St-Pée-sur-Nivelle,France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Médale
authors.affiliation: INRA, UR1067 NuMéA,F-64310St-Pée-sur-Nivelle,France.
authors.forename: Sadasivam J
authors.initials: SJ
authors.lastname: Kaushik
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D001823:Body Composition; D004032:Diet / Q000662:veterinary*; D019587:Dietary Supplements; D005227:Fatty Acids / Q000032:analysis; D015870:Gene Expression / Q000187:drug effects; D005978:Glutathione / Q000032:analysis / Q000737:chemistry; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism; D015227:Lipid Peroxidation; D009751:Nutritional Requirements; D017686:Oncorhynchus mykiss / Q000196:embryology / Q000254:growth & development / Q000502:physiology*; D018384:Oxidative Stress / Q000187:drug effects*; D012643:Selenium / Q000008:administration & dosage*; D051140:Selenoproteins / Q000235:genetics; D018038:Sodium Selenite
chemical_list: D000975:Antioxidants; D005227:Fatty Acids; D051140:Selenoproteins; D005979:Glutathione Peroxidase; D005978:Glutathione; D012643:Selenium; D018038:Sodium Selenite
keywords: Glutathione peroxidase; Oxidative stress; Rainbow trout fry; Selenium