title: Effect of light intensity on physiological changes, carbon allocation and neutral lipid accumulation in oleaginous microalgae.
abstract: Chlorella sp. and Monoraphidium sp. were the potential microalgal species for lipid production. This study aimed to investigate different light intensities (40, 200, 400 μmol photon m(-2) s(-1)) on physiological changes, photosynthetic carbon partitioning and neutral lipid accumulation in both microalgae. Results suggested that under high light (HL, 400 μmol photon m(-2) s(-1)), chlorophyll degraded, protein and carbohydrate content decreased; more carbon allocated into lipid as well as most of intracellular space was occupied by lipid bodies. Moreover, with the lipid accumulation, Fv/Fm decreased and ROS scavenging enzyme increased. Membrane lipid reduced dramatic (29.73-37.97%) to format NL (71.66% of total lipid in Chlorella sp. L1 and 60.65% in Monoraphidium dybowskii Y2). The NL productivity under HL (51.36 and 49.71 mg L(-1) d(-1)) were more than 3 times of those under LL. Additionally, FAME profiles proved that the useful fatty acid components for biodiesel production were enhanced under HL.
authors.affiliation: Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Qiaoning
authors.initials: Q
authors.lastname: He
authors.affiliation: Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Haijian
authors.initials: H
authors.lastname: Yang
authors.affiliation: Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wu
authors.affiliation: Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China. Electronic address: cxhu@ihb.ac.cn.
authors.forename: Chunxiang
authors.initials: C
authors.lastname: Hu
mesh_terms: D018533:Biomass; D002244:Carbon / Q000378:metabolism*; D008027:Light; D050356:Lipid Metabolism; D058086:Microalgae / Q000378:metabolism* / Q000502:physiology; D010788:Photosynthesis; D010860:Pigments, Biological / Q000378:metabolism
chemical_list: D010860:Pigments, Biological; D002244:Carbon
keywords: Carbon allocation; Light intensity; Microalgae; Neutral lipid accumulation; Physiology changes