title: Lectin-binding sites in epithelial cells of the mouse prostate gland.
abstract: The prostate is an exocrine gland in the male reproductive tract that secretes seminal fluids. To gain insight into the cytochemical properties of prostatic epithelial cells, the characteristics of glycoconjugates in mouse prostate sections were examined by lectin histochemistry and immunohistochemistry. Characteristic staining patterns were observed, depending on the type of lectins present in the epithelia. Luminal cells reacted specifically with mannose-binding lectins (Galanthus nivalis lectin, Hippeastrum hybrid lectin, Narcissus pseudonarcissus lectin) and Maclura pomifera lectin in all lobes of the prostate. Luminal cells also expressed galactose, N-acetyl-D-galactosamine (GalNAc), N-acetyl-D-glucosamine (GlcNAc), and fucose residues in the lateral and ventral lobes. Basal cells expressed GlcNAc and fucose, and reacted with Datura stramonium lectin and Aleuria aurantia lectin in all lobes. These results indicate that in the mouse prostate, the selectivity of lectin-binding sites for distinct cell types and lobe-dependent staining may relate to cellular and regional differences in function. Furthermore, some lectins selectively bound to prostatic epithelial cells, indicating their potential use as markers for the histopathological evaluation of prostatic diseases, cancer diagnosis, or male infertility.
authors.affiliation: Department of Anatomy and Cell Biology, Graduate School of Health Sciences, Kumamoto University.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Sakuda
authors.forename: Ayaka
authors.initials: A
authors.lastname: Yoshida
authors.forename: Ryoki
authors.initials: R
authors.lastname: Muragishi
authors.forename: Kazuya
authors.initials: K
authors.lastname: Yoshinaga
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D037102:Lectins / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011467:Prostate / Q000166:cytology / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D037102:Lectins