title: Oligonucleotide Probes for ND-FISH Analysis to Identify Rye and Wheat Chromosomes.
abstract: Genomic in situ hybridization (GISH) has been widely used to detect rye (Secale cereale L.) chromosomes in wheat (Triticum aestivum L.) introgression lines. The routine procedure of GISH using genomic DNA of rye as a probe is time-consuming and labor-intensive because of the preparation and labeling of genomic DNA of rye and denaturing of chromosomes and probes. In this study, new oligonucleotide probes Oligo-1162, Oligo-pSc200 and Oligo-pSc250 were developed. The three new probes can be used for non-denaturing fluorescence in situ hybridization (ND-FISH) assays and replace genomic DNA of rye as a probe to discriminate rye chromosomes in wheat backgrounds. In addition, previously developed oligonucleotide probes Oligo-pSc119.2-1, Oligo-pSc119.2-2, Oligo-pTa535-1, Oligo-pTa535-2, Oligo-pTa71-2, Oligo-pAWRC.1 and Oligo-CCS1 can also be used for ND-FISH of wheat and rye. These probes have provided an easier, faster and more cost-effective method for the FISH analysis of wheat and hybrids derived from wheat × rye.
authors.affiliation: 1] Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China [2] Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China.
authors.forename: Shulan
authors.initials: S
authors.lastname: Fu
authors.affiliation: 1] Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China [2] Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.affiliation: 1] Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China [2] Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China.
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: 1] Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China [2] Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China.
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, People's Republic of China.
authors.forename: Zujun
authors.initials: Z
authors.lastname: Yang
authors.affiliation: 1] Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China [2] Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China.
authors.forename: Ling
authors.initials: L
authors.lastname: Qiu
authors.affiliation: Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China.
authors.forename: Benju
authors.initials: B
authors.lastname: Yan
authors.affiliation: 1] Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China [2] Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China.
authors.forename: Zhenglong
authors.initials: Z
authors.lastname: Ren
authors.affiliation: 1] Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China [2] Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China.
authors.forename: Zongxiang
authors.initials: Z
authors.lastname: Tang
mesh_terms: D032461:Chromosomes, Plant / Q000235:genetics / Q000378:metabolism*; D017404:In Situ Hybridization, Fluorescence; D008856:Microscopy, Fluorescence; D015345:Oligonucleotide Probes / Q000235:genetics / Q000378:metabolism*; D011123:Polyploidy; D012434:Secale / Q000235:genetics*; D014908:Triticum / Q000235:genetics*
chemical_list: D015345:Oligonucleotide Probes