title: A Metalloproteomics Study on the Association of Mercury With Breast Milk in Samples From Lactating Women in the Amazon Region of Brazil.
abstract: This study aimed to identify metalloproteins that lose their metal ions in the presence of mercury (Hg) and bind to Hg in breast milk samples collected from the riverine population of the Madeira River, a tributary of the Amazon River. Initially, total Hg was determined from the hair of lactating women to identify individuals who were contaminated followed by a proteomic analysis of breast milk samples through two-dimensional polyacrylamide gel electrophoresis after acetone precipitation. The presence of Hg in the obtained protein spots was determined by cold vapor atomic absorption spectrometry and cold vapor atomic fluorescence spectrometry. These determinations indicated the presence of Hg in one protein spot, which was then characterized through electrospray ionization tandem mass spectrometry. Based on searches in the UniProt database, this protein spot was identified as lysozyme C.
authors.affiliation: Institute of Chemistry, Federal University of Mato Grosso do Sul (INQUI/UFMS), Campo Grande, Mato Grosso do Sul, Brazil.
authors.forename: Felipe André
authors.initials: FA
authors.lastname: dos Santos
authors.forename: Bruna
authors.initials: B
authors.lastname: Cavecci
authors.forename: José Cavalcante Souza
authors.initials: JC
authors.lastname: Vieira
authors.forename: Vanessa Pezza
authors.initials: VP
authors.lastname: Franzini
authors.forename: Ademir
authors.initials: A
authors.lastname: Santos
authors.forename: Aline
authors.initials: A
authors.lastname: de Lima Leite
authors.forename: Marília Afonso Rabelo
authors.initials: MA
authors.lastname: Buzalaf
authors.forename: Luiz Fabrício
authors.initials: LF
authors.lastname: Zara
authors.forename: Pedro
authors.initials: P
authors.lastname: de Magalhães Padilha
mesh_terms: D000328:Adult; D001938:Brazil; D015180:Electrophoresis, Gel, Two-Dimensional; D004785:Environmental Pollutants / Q000032:analysis / Q000378:metabolism*; D005260:Female; D006197:Hair / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007774:Lactation; D018811:Maternal Exposure / Q000706:statistics & numerical data*; D008628:Mercury / Q000032:analysis / Q000378:metabolism*; D008895:Milk, Human / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics
chemical_list: D004785:Environmental Pollutants; D008628:Mercury