title: Molecular-based diagnosis of respiratory allergic diseases in children from Curitiba, a city in Southern Brazil.
abstract: BACKGROUND
  Prevalence of respiratory allergic diseases has increased worldwide. Identification of the aeroallergens involved in allergenic sensitisation is important for diagnosis, treatment and prevention.
  
  
  OBJECTIVE
  To verify the molecular pattern of sensitisation to aeroallergens in patients with allergic respiratory diseases using microarray technique for specific IgE antibody detection.
  
  
  METHODS
  Cross-sectional study of 101 children with allergic rhinitis was followed in an outpatient clinic. All patients had positive skin prick tests (SPT) to at least one of the following antigens: Dermatophagoides pteronyssinus, Blomia tropicalis, Blattella germanica, Lolium multiflorum, and dog and cat epithelium. Serum specific IgE antibodies (sIgE) to mites, animal epithelia, fungi, cockroach and pollens components were determined by ImmunoCAP ISAC.
  
  
  RESULTS
  sIgE to group 1 and 2 mite allergens showed higher positive rates: Der p 1 (74.2%), Der p 2 (73.3%), Der f 1 (74.2%), Der f 2 (72.3%). sIgE to animal epithelia were less frequent, Can f 1, Can f 2, Can f 3 in 4.9%, 2.9%, 1.9% respectively and Fel d 1, Fel d 2, Fel d 4 in 16.8%, 0.9% and 1.9%. respectively. Sensitisation to fungi and cockroach were rare, except for Bla g 7, to which 16.8% were positive. There was no significant recognition for tree pollens group. For grass, sIgE were detected to Cyn d 1 in 16.8%, Phl p 1 and Phl p 4 in 14.8% and 12.9%, respectively.
  
  
  CONCLUSIONS
  Knowing that the pattern of allergic sensitisation varies according to environment and population, our results reinforce the need for local studies, using molecular-based diagnosis.
authors.affiliation: Federal University of Parana, Brazil. Electronic address: laura.araujo80@gmail.com.
authors.forename: L M L
authors.initials: LM
authors.lastname: Araujo
authors.affiliation: Federal University of Parana, Brazil.
authors.forename: N A
authors.initials: NA
authors.lastname: Rosario
authors.affiliation: Center for Molecular Allergology, IDI-IRCCS, Rome, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Mari
mesh_terms: D000293:Adolescent; D000393:Air Pollutants / Q000276:immunology / Q000378:metabolism*; D000485:Allergens / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000276:immunology; D060829:Arthropod Proteins / Q000276:immunology; D001938:Brazil; D002648:Child; D003430:Cross-Sectional Studies; D003546:Cysteine Endopeptidases / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007159:Immunologic Tests; D046228:Microarray Analysis; D057089:Pathology, Molecular; D039981:Pyroglyphidae / Q000276:immunology; D065631:Rhinitis, Allergic / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000393:Air Pollutants; D000485:Allergens; D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; D007073:Immunoglobulin E; D003546:Cysteine Endopeptidases; C451159:Dermatophagoides farinae antigen f 1
keywords: Allergens; Asthma; Children; Molecular diagnostic testing; Rhinitis