title: Pharmacokinetic investigation on interaction between hydrophilic lithospermic acid B and lipophilic tanshinone IIA in rats: an experi- mental study.
abstract: OBJECTIVE
  To elucidate the interaction between hydrophilic lithospermic acid B and lipophilic tanshinone II A in rats.
  
  
  METHODS
  A reliable high-performance liquid chromatography method was adopted for simultaneous determination of lithospermic acid B and tanshinone II A in rat plasma, through which the pharmacokinetic interaction between lithospermic acid B and tanshinone II A by intravenous injection was investigated.
  
  
  RESULTS
  The simultaneous intravenous injection of tanshinone II A and lithospermic acid B significantly altered the pharmacokinetic parameters of both compounds when compared with the individual intravenous administration of each compound. The area under the concentration-time curve of tanshinone II A and lithospermic acid B increased by 18.35 and 59.31%, respectively. The mean retention time of tanshinone II A and lithospermic acid B increased, respectively, from 9.3 to 32.8 h and 20.2 to 49.1 h. The concomitant use of tanshinone II A magnified the volume of distribution at steady state (Vss) and time for the drug in the plasma to reduce the highest concentration by half (t½) of lithospermic acid B, while at the same time the Vss and t½ of tanshinone II A changed significantly in the presence of lithospermic acid B.
  
  
  CONCLUSIONS
  Lithospermic acid B and tanshinone II A interact with each other following simultaneous intravenous injection in rats and this observation may expand the clinical use of Danshen (Radix Salviae Miltiorrhizae).
authors.forename: Wang
authors.initials: W
authors.lastname: Xiuli
authors.forename: Gao
authors.initials: G
authors.lastname: Wei
authors.forename: Sun
authors.initials: S
authors.lastname: Mao
mesh_terms: D045784:Abietanes / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D000818:Animals; D001572:Benzofurans / Q000097:blood / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid; D053630:Depsides / Q000097:blood / Q000493:pharmacokinetics*; D004347:Drug Interactions; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D045784:Abietanes; D001572:Benzofurans; D053630:Depsides; D004365:Drugs, Chinese Herbal; C021751:tanshinone; C076944:salvianolic acid B