title: Interactions of short-acting, intermediate-acting and pre-mixed human insulins with free radicals--Comparative EPR examination.
abstract: Electron paramagnetic resonance (EPR) spectroscopy was used to examine insulins interactions with free radicals. Human recombinant DNA insulins of three groups were studied: short-acting insulin (Insuman Rapid); intermediate-acting insulins (Humulin N, Insuman Basal), and pre-mixed insulins (Humulin M3, Gensulin M50, Gensulin M40, Gensulin M30). The aim of an X-band (9.3GHz) study was comparative analysis of antioxidative properties of the three groups of human insulins. DPPH was used as a stable free radical model. Amplitudes of EPR lines of DPPH as the paramagnetic free radical reference, and DPPH interacting with the individual tested insulins were compared. For all the examined insulins kinetics of their interactions with free radicals up to 60 min were obtained. The strongest interactions with free radicals were observed for the short-acting insulin - Insuman Rapid. The lowest interactions with free radicals were characteristic for intermediate-acting insulin - Insuman Basal. The pre-mixed insulins i.e. Humulin M3 and Gensulin M50 revealed the fastest interactions with free radicals. The short acting, intermediate acting and premixed insulins have been found to be effective agents in reducing free radical formation in vitro and should be further considered as potential useful tools in attenuation of oxidative stress in diabetic patients.
authors.affiliation: Department of Community Pharmacy, School of Pharmacy and Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Kasztanowa 3, 41-200 Sosnowiec, Poland. Electronic address: polczyk@sum.edu.pl.
authors.forename: Paweł
authors.initials: P
authors.lastname: Olczyk
authors.affiliation: Department of Clinical Chemistry and Laboratory Diagnostics, School of Pharmacy and Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Jedności 8, 41-200 Sosnowiec, Poland. Electronic address: kvassev@sum.edu.pl.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Komosinska-Vassev
authors.affiliation: Department of Biophysics, School of Pharmacy and Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Jedności 8, 41-200 Sosnowiec, Poland. Electronic address: pawelramos@sum.edu.pl.
authors.forename: Paweł
authors.initials: P
authors.lastname: Ramos
authors.affiliation: Department of Clinical Chemistry and Laboratory Diagnostics, School of Pharmacy and Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Jedności 8, 41-200 Sosnowiec, Poland. Electronic address: lmencner@sum.edu.pl.
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Mencner
authors.affiliation: Department of Clinical Chemistry and Laboratory Diagnostics, School of Pharmacy and Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Jedności 8, 41-200 Sosnowiec, Poland. Electronic address: olczyk@sum.edu.pl.
authors.forename: Krystyna
authors.initials: K
authors.lastname: Olczyk
authors.affiliation: Department of Biophysics, School of Pharmacy and Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Jedności 8, 41-200 Sosnowiec, Poland. Electronic address: bpilawa@sum.edu.pl.
authors.forename: Barbara
authors.initials: B
authors.lastname: Pilawa
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D005609:Free Radicals / Q000378:metabolism*; D006801:Humans; D061386:Insulin, Regular, Human / Q000494:pharmacology*; D000068880:Isophane Insulin, Human / Q000494:pharmacology*; D007700:Kinetics; D018384:Oxidative Stress / Q000187:drug effects
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D061386:Insulin, Regular, Human; D000068880:Isophane Insulin, Human
keywords: Antioxidant; DPPH; EPR spectra; EPR spectroscopy; Free radicals; Insulin