title: Interleukin 27 -964A > G genetic polymorphism and serum IL-27p28 levels in Chinese patients with papillary thyroid cancer.
abstract: The aim of this study was to investigate the association between a potentially functional polymorphism (rs153109, -964A > G) in the promoter region of interleukin-27 (IL-27) gene and the risk of papillary thyroid cancer (PTC) in a Chinese population. Genotype of IL-27 -964A > G polymorphism was determined using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. Serum IL-27p28 levels were determined using enzyme-linked immunosorbent assay (ELISA). No significant difference was noticed in IL-27 -964A > G polymorphism between PTC patients and healthy controls in the overall analysis. However, analysis of clinical features showed that PTC patients carrying the GG genotype or G allele had significantly decreased risks for developing lymph node metastasis compared with those carrying the AA genotype or A allele (GG vs. AA: OR = 0.38, 95 % CI, 0.20-0.72; G vs. A: OR = 0.63, 95 % CI, 0.44-0.86). Furthermore, ELISA results demonstrated that serum IL-27p28 levels were significantly decreased in PTC patients compared with those in controls (P < 0.05). Serum IL-27p28 levels in healthy controls with the GG genotype were significantly high compared with those carrying AA genotype or the AG genotype (P < 0.05). In conclusion, our results suggest that IL-27 -964A > G polymorphism may be associated with the decreased risk for lymph node metastasis of PTC.
authors.affiliation: Department of General Surgery, Zhongda Hospital, Medical School of Southeast University, Nanjing, 210009, Jiangsu, China.
authors.forename: Shulong
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Microbiology and Immunology, Medical College of Southeast University, Nanjing, 21009, Jiangsu, China.
authors.forename: Xueren
authors.initials: X
authors.lastname: Gao
authors.affiliation: Department of Interventional Radiology and Vascular Surgery, Medical School of Southeast University, Nanjing, 210009, Jiangsu, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Surgical Oncology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, 233004, Anhui, China.
authors.forename: Jianguang
authors.initials: J
authors.lastname: Jia
authors.affiliation: Department of Clinical Science, The First Affiliated Hospital of Bengbu Medical College, Bengbu, 233004, Anhui, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, Zhongda Hospital, Medical School of Southeast University, Nanjing, 210009, Jiangsu, China. chinaren0518@163.com.
authors.forename: Zhenling
authors.initials: Z
authors.lastname: Ji
mesh_terms: D000231:Adenocarcinoma, Papillary / Q000097:blood* / Q000235:genetics* / Q000556:secondary; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005500:Follow-Up Studies; D005838:Genotype; D006801:Humans; D007378:Interleukins / Q000097:blood* / Q000235:genetics*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011379:Prognosis; D013961:Thyroid Gland / Q000378:metabolism; D013964:Thyroid Neoplasms / Q000097:blood* / Q000235:genetics* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D007378:Interleukins; C464565:MYDGF protein, human
keywords: IL-27; Papillary thyroid cancer; Polymorphism; Risk