title: Characterization of efflux transport proteins of the human choroid plexus papilloma cell line HIBCPP, a functional in vitro model of the blood-cerebrospinal fluid barrier.
abstract: OBJECTIVE
  To characterize the human choroid plexus (CP) papilloma cell line HIBCPP with respect to ABC export protein expression and function in order to evaluate its use as an in vitro model to study carrier-mediated transport processes at the CP.
  
  
  METHODS
  Expression profiles of ABC transporters were studied by quantitative real-time PCR and Western Blot analysis. Functionality of transporters was investigated by means of uptake experiments and permeation studies carried out on permeable filter systems. In addition, immunohistochemistry served to study localization of ABCC1 and ABCC4.
  
  
  RESULTS
  Both qPCR and Western Blot revealed that ABC transporters known to be expressed in CP are also expressed in HIBCPP cells. Immunohistochemistry confirmed basolateral expression of ABCC1. Functionality of ABCC1, ABCC4, ABCB1 and ABCG2 could be shown in uptake assays.
  
  
  CONCLUSIONS
  Altogether, the HIBCPP cells promise to be a functional and relevant in vitro tool to investigate transport processes at the blood-cerebrospinal fluid barrier.
authors.affiliation: Institute of Pharmacy and Molecular Biotechnology, Ruprecht-Karls University Heidelberg, Im Neuenheimer Feld 329, 69120, Heiderlberg, Germany.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Bernd
authors.forename: Melanie
authors.initials: M
authors.lastname: Ott
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ishikawa
authors.forename: Horst
authors.initials: H
authors.lastname: Schroten
authors.forename: Christian
authors.initials: C
authors.lastname: Schwerk
authors.forename: Gert
authors.initials: G
authors.lastname: Fricker
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000378:metabolism; D001692:Biological Transport / Q000502:physiology; D001812:Blood-Brain Barrier / Q000378:metabolism*; D045744:Cell Line, Tumor; D002831:Choroid Plexus / Q000378:metabolism*; D006801:Humans; D026901:Membrane Transport Proteins / Q000378:metabolism; D020288:Papilloma, Choroid Plexus / Q000378:metabolism*
chemical_list: D018528:ATP-Binding Cassette Transporters; D026901:Membrane Transport Proteins