title: Chiral resolution capabilities of DNA oligonucleotides.
abstract: Herein, we studied the chiral resolution properties of a repertoire of arbitrarily chosen DNA oligonucleotides (ON). Ten oligonucleotidic sequences characterized by diverse base compositions, sizes, and structural features, ranging from secondary structure-free homo-oligonucleotides to duplex, hairpin, and three-way junction architectures, were investigated as potential chiral selectors. Their enantioselective features were assessed by using ONs as running buffer additives in partial-filling capillary electrophoresis. It was shown that all the screened sequences displayed enantiodiscrimination capabilities toward small aromatic compounds. Under (sub)millimolar DNA concentration conditions, the combination of only three oligonucleotidic sequences provided the chiral resolution of around 20 racemates, including drugs, illegal drugs, amino-acids, and nucleosides. This work represents the first demonstration of such analyte selectivity spectrum for nucleic acid-based chiral separation tools.
authors.affiliation: Université Grenoble Alpes, DPM UMR 5063, F-38041 Grenoble, CNRS, DPM UMR 5063, F-38041 Grenoble, France.
authors.forename: Luma
authors.initials: L
authors.lastname: Tohala
authors.affiliation: Université Grenoble Alpes, DPM UMR 5063, F-38041 Grenoble, CNRS, DPM UMR 5063, F-38041 Grenoble, France.
authors.forename: Farid
authors.initials: F
authors.lastname: Oukacine
authors.affiliation: Université Grenoble Alpes, DPM UMR 5063, F-38041 Grenoble, CNRS, DPM UMR 5063, F-38041 Grenoble, France.
authors.forename: Corinne
authors.initials: C
authors.lastname: Ravelet
authors.affiliation: Université Grenoble Alpes, DPM UMR 5063, F-38041 Grenoble, CNRS, DPM UMR 5063, F-38041 Grenoble, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Peyrin
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000379:methods* / Q000592:standards*; D004247:DNA / Q000737:chemistry*; D015394:Molecular Structure; D009841:Oligonucleotides / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: D009841:Oligonucleotides; D004247:DNA