title: Characterization of the interaction of FTO protein with thioglycolic acid capped CdTe quantum dots and its analytical application.
abstract: CdTe quantum dots (QDs) were synthesized in aqueous solution using thioglycolic acid (TGA) as stabilizing agents. The interaction between TGA-CdTe QDs and fat mass and obesity-associated (FTO) protein was investigated by fluorescence, UV-visible absorption, synchronous fluorescence and three-dimensional fluorescence spectroscopy. Results revealed that TGA-CdTe QDs could strongly quench the intrinsic fluorescence of FTO protein with a static quenching procedure. Both the van der Waals and hydrogen bonding played a major role in stabilizing the complex. The binding constant and thermodynamic parameters at different temperatures were obtained. In addition, we found that the fluorescence intensity of QDs was significantly enhanced by the addition of FTO protein. Based on this, a sensitive method for detecting FTO protein was obtained in the linear range of 5.52×10(-9)-6.62×10(-7) mol L(-1) with the detection limit of 1.14×10(-9) mol L(-1). The influences of factors on the interaction between FTO protein and TGA-CdTe QDs were studied.
authors.affiliation: Department of Chemistry, Zhengzhou University, Zhengzhou, China.
authors.forename: Baoyu
authors.initials: B
authors.lastname: Ge
authors.affiliation: Department of Chemistry, Zhengzhou University, Zhengzhou, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Chemistry, Zhengzhou University, Zhengzhou, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Chemistry, Zhengzhou University, Zhengzhou, China. Electronic address: wangry@zzu.edu.cn.
authors.forename: Ruiyong
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Zhengzhou University, Zhengzhou, China. Electronic address: changjunbiao@zzu.edu.cn.
authors.forename: Junbiao
authors.initials: J
authors.lastname: Chang
mesh_terms: D019187:Cadmium Compounds / Q000378:metabolism*; D002138:Calibration; D007700:Kinetics; D011506:Proteins / Q000378:metabolism*; D045663:Quantum Dots / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013691:Tellurium / Q000378:metabolism*; D013696:Temperature; D013864:Thioglycolates / Q000378:metabolism*; D013997:Time Factors
chemical_list: D019187:Cadmium Compounds; D011506:Proteins; D013864:Thioglycolates; C017487:2-mercaptoacetate; D013691:Tellurium; C028337:cadmium telluride
keywords: CdTe quantum dots; Determination; FTO protein; Fluorescence; Interaction