title: Multispectroscopic DNA-binding studies of a terbium(III) complex containing 2,2'-bipyridine ligand.
abstract: Agarose gel electrophoresis, absorption, fluorescence, viscosity, and circular dichroism (CD) have been used in exploring the interaction of terbium(III) complex, [Tb(bpy)2Cl3(OH2)] where bipy is 2,2'-bipyridine, with Fish salmon DNA. Agarose gel electrophoresis assay, along with absorption and fluorescence studies, reveal interaction between the corresponding complex and FS-DNA. Also, the binding constants (Kb) and the Stern-Volmer quenching constants (Ksv) of Tb(III) complex with FS-DNA were determined. The calculated thermodynamic parameters suggested that the binding of mentioned complex to FS-DNA was driven mainly by hydrophobic interactions. A comparative study of this complex with respect to the effect of iodide-induced quenching, ionic strength effect, and ethidium bromide exclusion assay reflects binding of explicit to the FS-DNA primarily in a groove fashion. CD and viscosity data also support the groove binding mode. Furthermore, Tb(III) complex have been simultaneously screened for their antibacterial and antifungal activities.
authors.affiliation: a Department of Chemistry , University of Sistan and Baluchestan , P.O. Box 98155-147, Zahedan , Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Aramesh-Boroujeni
authors.affiliation: a Department of Chemistry , University of Sistan and Baluchestan , P.O. Box 98155-147, Zahedan , Iran.
authors.forename: Mozhgan
authors.initials: M
authors.lastname: Khorasani-Motlagh
authors.affiliation: a Department of Chemistry , University of Sistan and Baluchestan , P.O. Box 98155-147, Zahedan , Iran.
authors.forename: Meissam
authors.initials: M
authors.lastname: Noroozifar
mesh_terms: D015082:2,2'-Dipyridyl / Q000494:pharmacology*; D065638:Absorption, Radiation; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D001665:Binding Sites; D002942:Circular Dichroism; D004247:DNA / Q000378:metabolism*; D053837:DNA Cleavage; D004587:Electrophoresis, Agar Gel; D004996:Ethidium / Q000737:chemistry / Q000378:metabolism; D005658:Fungi / Q000187:drug effects; D007700:Kinetics; D008024:Ligands; D049449:Luminescence; D008826:Microbial Sensitivity Tests; D009994:Osmolar Concentration; D012474:Salmon; D013050:Spectrometry, Fluorescence; D013696:Temperature; D013725:Terbium / Q000494:pharmacology*; D014783:Viscosity
chemical_list: D000890:Anti-Infective Agents; D008024:Ligands; D013725:Terbium; D015082:2,2'-Dipyridyl; D004247:DNA; D004996:Ethidium
keywords: 2,2′-bipyridine; DNA-binding; groove binding; spectroscopy; terbium(III) complex; thermodynamic parameters