title: [The vitro research of effects of Beclin1 on paclitaxel-sensitivity in laryngeal carcinoma cell Hep-2].
abstract: OBJECTIVE
  Background: We detect the effects of Beclinl on paclitaxel-sensitivity in laryngeal carcinoma cell.
  
  
  METHODS
  This study used Hep-2, Hep-2-pcDNA3. 1, Hep-2-Beclinl as invitro model. The effect of paclitaxel on the proliferation and cell apoptosis of laryngeal cancer cell lines was evaluated by MTT assay and flow cytometry. The protein expression level of Akt and p-Akt was detected by Western blot. Result: After treated by paclitaxel, the inhibition rate was significantly higher in Hep-2-Beclin cells than in Hep-2-pcDNA3. 1 cells and Hep-2 cells (P<. 05). After dealing with 10 tg/L paclitaxel, the apoptosis rate in Hep-2, Hep-2-pcDNA3. 1, Hep-2-Beclinl were (23. 75 ± 2 3. 77) %, (21. 25 ± 4. 92) %, (32. 50 ± 5. 97) %, respectively. After dealing with 20µg/L paclitaxel, the apoptosis rate in Hep-2, Hep-2-pcDNA3. 1, Hep-2-Beclinl were (38. 75 ± 4. 79) %, (38. 75±6. 55) %, (50. 00±7. 26) %, respectively. Paclitaxel-induced apoptosis was higher in Hep-2-Beclin cells than in Hep-2-pcDNA3. 1 cells and Hep-2 cells (P<. 05). The result of western blot showed that the protein expression level of p-Akt in Hep-2-Beclin cells was lower than in Hep-2-pcDNA3. 1 cells and Hep-2 cells (P<0. 05) and the protein expression level of Akt was similar in three cell lines (P>0. 05).
  
  
  CONCLUSIONS
  Beclinl enhances paclitaxel-sensitivity by inhibition of PI3K/Akt pathway.
authors.forename: Xiaocong
authors.initials: X
authors.lastname: Deng
authors.forename: Xinming
authors.initials: X
authors.lastname: Yang
authors.forename: Shisheng
authors.initials: S
authors.lastname: Li
mesh_terms: D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000502:physiology*; D000071186:Beclin-1; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D007822:Laryngeal Neoplasms / Q000473:pathology*; D007830:Larynx; D008565:Membrane Proteins / Q000502:physiology*; D017239:Paclitaxel / Q000494:pharmacology*; D019869:Phosphatidylinositol 3-Kinases
chemical_list: D051017:Apoptosis Regulatory Proteins; C491997:BECN1 protein, human; D000071186:Beclin-1; D008565:Membrane Proteins; D019869:Phosphatidylinositol 3-Kinases; D017239:Paclitaxel