title: Segregation versus colocalization: orthogonally functionalized binary micropatterned substrates regulate the molecular distribution in focal adhesions.
abstract: Orthogonally functionalized binary micropatterned substrates are produced using a novel protocol. The use of adequate peptido-mimetics enables an unprecedented segregation of purified αvβ3 and α5β1 integrins in adjacent microislands and evidences the preference of U2OS cells to colocalize such receptors. Moreover, this tendency can be altered by varying the geometry and composition of the micropatterns.
authors.affiliation: Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems, Heisenbergstr. 3, Stuttgart, D-70569, Germany.|Department of Biophysical Chemistry, University of Heidelberg INF 253, Heidelberg, D-69120, Germany.
authors.forename: Judith
authors.initials: J
authors.lastname: Guasch
authors.affiliation: Institute for Materials Research, Wetenschapspark 1, Diepenbeek, B-3590, Belgium.
authors.forename: Bert
authors.initials: B
authors.lastname: Conings
authors.affiliation: Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems, Heisenbergstr. 3, Stuttgart, D-70569, Germany.|Institute for Advanced Study (IAS) and Center of Integrated Protein Science (CIPSM), Department Chemie, Technische Universität München, Lichtenbergstr. 4, Garching, D-85747, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Neubauer
authors.affiliation: Institute for Advanced Study (IAS) and Center of Integrated Protein Science (CIPSM), Department Chemie, Technische Universität München, Lichtenbergstr. 4, Garching, D-85747, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Rechenmacher
authors.affiliation: Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems, Heisenbergstr. 3, Stuttgart, D-70569, Germany.
authors.forename: Karen
authors.initials: K
authors.lastname: Ende
authors.affiliation: Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems, Heisenbergstr. 3, Stuttgart, D-70569, Germany.|Department of Biophysical Chemistry, University of Heidelberg INF 253, Heidelberg, D-69120, Germany.
authors.forename: Claudio G
authors.initials: CG
authors.lastname: Rolli
authors.affiliation: Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems, Heisenbergstr. 3, Stuttgart, D-70569, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Kappel
authors.affiliation: Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems, Heisenbergstr. 3, Stuttgart, D-70569, Germany.|Department of Biophysical Chemistry, University of Heidelberg INF 253, Heidelberg, D-69120, Germany.
authors.forename: Viktoria
authors.initials: V
authors.lastname: Schaufler
authors.affiliation: Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems, Heisenbergstr. 3, Stuttgart, D-70569, Germany.|Department of Biophysical Chemistry, University of Heidelberg INF 253, Heidelberg, D-69120, Germany.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Micoulet
authors.affiliation: Institute for Advanced Study (IAS) and Center of Integrated Protein Science (CIPSM), Department Chemie, Technische Universität München, Lichtenbergstr. 4, Garching, D-85747, Germany.
authors.forename: Horst
authors.initials: H
authors.lastname: Kessler
authors.affiliation: Institute for Materials Research, Wetenschapspark 1, Diepenbeek, B-3590, Belgium.
authors.forename: Hans-Gerd
authors.initials: HG
authors.lastname: Boyen
authors.affiliation: Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems, Heisenbergstr. 3, Stuttgart, D-70569, Germany.|Department of Biophysical Chemistry, University of Heidelberg INF 253, Heidelberg, D-69120, Germany.
authors.forename: Elisabetta Ada
authors.initials: EA
authors.lastname: Cavalcanti-Adam
authors.affiliation: Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems, Heisenbergstr. 3, Stuttgart, D-70569, Germany.|Department of Biophysical Chemistry, University of Heidelberg INF 253, Heidelberg, D-69120, Germany.
authors.forename: Joachim P
authors.initials: JP
authors.lastname: Spatz
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism; D040761:Biomimetic Materials; D002448:Cell Adhesion / Q000502:physiology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D002467:Cell Nucleus / Q000378:metabolism; D003470:Culture Media; D005290:Ferric Compounds; D022001:Focal Adhesions / Q000378:metabolism*; D006046:Gold; D006801:Humans; D039081:Integrin alpha5beta1 / Q000378:metabolism*; D039302:Integrin alphaVbeta3 / Q000378:metabolism*; D015394:Molecular Structure; D013499:Surface Properties; D014025:Titanium; D016596:Vinculin / Q000378:metabolism
chemical_list: D003470:Culture Media; D005290:Ferric Compounds; D039081:Integrin alpha5beta1; D039302:Integrin alphaVbeta3; D016596:Vinculin; C009495:titanium dioxide; C000499:ferric oxide; D006046:Gold; D014025:Titanium
keywords: biomimetics; cell adhesion and migration; integrins; micropatterns; orthogonal functionalization