title: Maturation and Mip-1β Production of Cytomegalovirus-Specific T Cell Responses in Tanzanian Children, Adolescents and Adults: Impact by HIV and Mycobacterium tuberculosis Co-Infections.
abstract: It is well accepted that aging and HIV infection are associated with quantitative and functional changes of CMV-specific T cell responses. We studied here the expression of Mip-1β and the T cell maturation marker CD27 within CMVpp65-specific CD4(+) and CD8(+) T cells in relation to age, HIV and active Tuberculosis (TB) co-infection in a cohort of Tanzanian volunteers (≤ 16 years of age, n = 108 and ≥ 18 years, n = 79). Independent of HIV co-infection, IFNγ(+) CMVpp65-specific CD4(+) T cell frequencies increased with age. In adults, HIV co-infection further increased the frequencies of these cells. A high capacity for Mip-1β production together with a CD27(low) phenotype was characteristic for these cells in children and adults. Interestingly, in addition to HIV co-infection active TB disease was linked to further down regulation of CD27 and increased capacity of Mip-1β production in CMVpp65-specific CD4+ T cells. These phenotypic and functional changes of CMVpp65-specific CD4 T cells observed during HIV infection and active TB could be associated with increased CMV reactivation rates.
authors.affiliation: Swiss Tropical and Public Health Institute, Basel, Switzerland; University of Basel, Basel, Switzerland.
authors.forename: Damien
authors.initials: D
authors.lastname: Portevin
authors.affiliation: NIMR-Mbeya Medical Research Centre, Mbeya, Tanzania; Department of Infectious Diseases and Tropical Medicine, Medical Centre of the University of Munich, Munich, Germany.
authors.forename: Félicien
authors.initials: F
authors.lastname: Moukambi
authors.affiliation: Ifakara Health Institute, Bagamoyo, Tanzania.
authors.forename: Maxmillian
authors.initials: M
authors.lastname: Mpina
authors.affiliation: NIMR-Mbeya Medical Research Centre, Mbeya, Tanzania; Department of Infectious Diseases and Tropical Medicine, Medical Centre of the University of Munich, Munich, Germany.
authors.forename: Asli
authors.initials: A
authors.lastname: Bauer
authors.affiliation: Ifakara Health Institute, Bagamoyo, Tanzania.
authors.forename: Frederick
authors.initials: F
authors.lastname: Haraka
authors.affiliation: NIMR-Mbeya Medical Research Centre, Mbeya, Tanzania.
authors.forename: Mkunde
authors.initials: M
authors.lastname: Chachage
authors.affiliation: Swiss Tropical and Public Health Institute, Basel, Switzerland.
authors.forename: Philipp
authors.initials: P
authors.lastname: Metzger
authors.affiliation: Department of Infectious Diseases and Tropical Medicine, Medical Centre of the University of Munich, Munich, Germany; German Centre for Infection Research (DZIF), partner site Munich, Munich, Germany.
authors.forename: Elmar
authors.initials: E
authors.lastname: Saathoff
authors.affiliation: NIMR-Mbeya Medical Research Centre, Mbeya, Tanzania; Department of Infectious Diseases and Tropical Medicine, Medical Centre of the University of Munich, Munich, Germany.
authors.forename: Petra
authors.initials: P
authors.lastname: Clowes
authors.affiliation: NIMR-Mbeya Medical Research Centre, Mbeya, Tanzania.
authors.forename: Nyanda E
authors.initials: NE
authors.lastname: Ntinginya
authors.affiliation: Department of Infectious Diseases and Tropical Medicine, Medical Centre of the University of Munich, Munich, Germany; German Centre for Infection Research (DZIF), partner site Munich, Munich, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Rachow
authors.affiliation: Department of Infectious Diseases and Tropical Medicine, Medical Centre of the University of Munich, Munich, Germany; German Centre for Infection Research (DZIF), partner site Munich, Munich, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Hoelscher
authors.affiliation: Swiss Tropical and Public Health Institute, Basel, Switzerland; University of Basel, Basel, Switzerland; Ifakara Health Institute, Bagamoyo, Tanzania.
authors.forename: Klaus
authors.initials: K
authors.lastname: Reither
authors.affiliation: Swiss Tropical and Public Health Institute, Basel, Switzerland; University of Basel, Basel, Switzerland.
authors.forename: Claudia A
authors.initials: CA
authors.lastname: Daubenberger
authors.affiliation: Department of Infectious Diseases and Tropical Medicine, Medical Centre of the University of Munich, Munich, Germany; German Centre for Infection Research (DZIF), partner site Munich, Munich, Germany.
authors.forename: Christof
authors.initials: C
authors.lastname: Geldmacher
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000821:virology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000821:virology; D054407:Chemokine CCL4 / Q000378:metabolism*; D002648:Child; D015331:Cohort Studies; D060085:Coinfection / Q000276:immunology; D003587:Cytomegalovirus / Q000276:immunology; D003586:Cytomegalovirus Infections / Q000150:complications / Q000276:immunology* / Q000821:virology; D015658:HIV Infections / Q000150:complications / Q000175:diagnosis / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D008297:Male; D010750:Phosphoproteins / Q000378:metabolism; D012307:Risk Factors; D013636:Tanzania; D014376:Tuberculosis / Q000150:complications / Q000175:diagnosis / Q000276:immunology*; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000378:metabolism; D014763:Viral Matrix Proteins / Q000378:metabolism; D055815:Young Adult
chemical_list: D054407:Chemokine CCL4; D010750:Phosphoproteins; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa; D007371:Interferon-gamma