title: First identification of Porcine Circovirus Type 2b mutant in pigs from Uruguay.
abstract: Porcine Circovirus Type 2 (PCV2) is a worldwide distributed virus and is considered an important emerging pathogen related to several distinct disease syndromes in pigs. PCV2 strains are classified into three genotypes: PCV2a, with five subtypes (2A-2E), PCV2b with three subtypes (1A-1C) and PCV2c, only found in Denmark. Recently, several reports suggested the circulation of newly emerging PCV2b mutants (mPCV2b) isolated from pigs with PCVAD in cases of suspected vaccine failure. In this work, we report for the first time the identification of mPCV2b in pigs from Uruguay, providing an additional evidence of a global circulation. Complete genome characterization and phylogenetic analysis reveal that Uruguayan strains, as well as mPCV2b previously reported are closely related to other sequences already classified as PCV2b-1C. Furthermore, results showed that mPCV2b presented different genetic markers in the capsid protein compared with classical PCV2a/b strains. Further investigation about antigenic shift of the mPCV2b strains including the Uruguayan isolates is needed.
authors.affiliation: Sección Virología, Facultad de Ciencias, Universidad de la República, Iguá 4225, Montevideo 11400, Uruguay. Electronic address: nramos@fcien.edu.uy.
authors.forename: Natalia
authors.initials: N
authors.lastname: Ramos
authors.affiliation: Sección Virología, Facultad de Ciencias, Universidad de la República, Iguá 4225, Montevideo 11400, Uruguay. Electronic address: smirazo@fcien.edu.uy.
authors.forename: Santiago
authors.initials: S
authors.lastname: Mirazo
authors.affiliation: División Sanidad Animal, Ministerio de Ganadería, Agricultura y Pesca, Constituyente 1476, Montevideo 11200, Uruguay. Electronic address: gucastro@mgap.gub.uy.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Castro
authors.affiliation: Sección Virología, Facultad de Ciencias, Universidad de la República, Iguá 4225, Montevideo 11400, Uruguay. Electronic address: jarbiza@fcien.edu.uy.
authors.forename: Juan
authors.initials: J
authors.lastname: Arbiza
mesh_terms: D000818:Animals; D018173:Circoviridae Infections / Q000662:veterinary*; D017925:Circovirus / Q000145:classification* / Q000235:genetics*; D005814:Genes, Viral; D016679:Genome, Viral; D005838:Genotype; D009154:Mutation; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D013552:Swine; D013553:Swine Diseases / Q000821:virology*; D014583:Uruguay
keywords: PCV2b mutant; PCV2b-1C genotype; Uruguay