title: Electrochemical evaluation of poly(3,4-ethylenedioxythiophene) films doped with bacteria based on viability analysis.
abstract: To immobilize viable bacteria on an electrode, we present a novel and straightforward technique that relies on the negative ζ-potentials of bacteria for insertion into conducting polymers as dopants. In the present study, we conducted an electrochemical polymerization of poly(3,4-ethylenedioxythiophene) (PEDOT) doped with various gram-negative bacteria, including Pseudomonas aeruginosa, Escherichia coli, and Shewanella oneidensis. The PEDOT film doped with bacteria indicated a typical redox response, high conductivity, and electrochemical stability. Fluorescence microscopy confirmed that approximately 90% of the bacteria incorporated into the PEDOT film at >0.5 μm in thickness were viable.
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, 1-2 Gakuen-cho, Naka, Sakai, Osaka 599-8570, Japan.
authors.forename: Dung Quynh
authors.initials: DQ
authors.lastname: Le
authors.affiliation: Nanoscience and Nanotechnology Research Center, Osaka Prefecture University, 1-2 Gakuen-cho, Naka, Sakai, Osaka 599-8570, Japan.
authors.forename: Shiho
authors.initials: S
authors.lastname: Tokonami
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, 1-2 Gakuen-cho, Naka, Sakai, Osaka 599-8570, Japan.
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Nishino
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, 1-2 Gakuen-cho, Naka, Sakai, Osaka 599-8570, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shiigi
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, 1-2 Gakuen-cho, Naka, Sakai, Osaka 599-8570, Japan. Electronic address: nagaoka@chem.osakafu-u.ac.jp.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Nagaoka
mesh_terms: D001419:Bacteria / Q000302:isolation & purification*; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry*; D055664:Electrochemical Techniques; D011108:Polymers / Q000737:chemistry*
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D011108:Polymers; C121383:poly(3,4-ethylene dioxythiophene)
keywords: Bacteria-doped PEDOT; Bacterial viability; Escherichia coli; Pseudomonas aeruginosa