title: Serum microRNAs explain discordance of non-alcoholic fatty liver disease in monozygotic and dizygotic twins: a prospective study.
abstract: In the setting where two individuals are genetically similar, epigenetic mechanisms could account for discordance in the presence or absence of non-alcoholic fatty liver disease (NAFLD). This study investigated if serum microRNAs (miRs) could explain discordance in NAFLD.
  
  
  
  This is a cross-sectional analysis of a prospective cohort study of 40 (n=80) twin-pairs residing in Southern California. All participants underwent a standardised research visit, liver MRI using proton-density fat fraction to quantify fat content and miR profiling of their serum.
  
  
  
  Among the 40 twin-pairs, there were 6 concordant for NAFLD, 28 were concordant for non-NAFLD and 6 were discordant for NAFLD. The prevalence of NAFLD was 22.5% (18/80). Within the six discordant twins, a panel of 10 miRs differentiated the twin with NAFLD from the one without. Two of these miRs, miR-331-3p and miR-30c, were also among the 21 miRs that were different between NAFLD and non-NAFLD groups (for miR-331-3p: 7.644±0.091 vs 8.057±0.071, respectively, p=0.004; for miR-30c: 10.013±0.126 vs 10.418±0.086, respectively, p=0.008). Both miRs were highly heritable (35.9% and 10.7%, respectively) and highly correlated with each other (R=0.90, p=2.2×10(-16)) suggesting involvement in a common mechanistic pathway. An interactome analysis of these two miRs showed seven common target genes.
  
  
  
  Using a novel human twin-study design, we demonstrate that discordancy in liver fat content between the twins can be explained by miRs, and that they are heritable.
authors.affiliation: NAFLD Translational Research Unit, University of California, San Diego, La Jolla, California, USA Division of Gastroenterology, Department of Medicine, University of California, San Diego, La Jolla, California, USA.
authors.forename: Amir
authors.initials: A
authors.lastname: Zarrinpar
authors.affiliation: Department of Bioengineering, San Diego Supercomputer Center, University of California, San Diego, La Jolla, California, USA.
authors.forename: Shakti
authors.initials: S
authors.lastname: Gupta
authors.affiliation: Department of Bioengineering, San Diego Supercomputer Center, University of California, San Diego, La Jolla, California, USA.
authors.forename: Mano R
authors.initials: MR
authors.lastname: Maurya
authors.affiliation: Department of Bioengineering, San Diego Supercomputer Center, University of California, San Diego, La Jolla, California, USA Departments of Computer Science & Engineering and Cellular & Molecular Medicine, University of California, San Diego, La Jolla, California, USA.
authors.forename: Shankar
authors.initials: S
authors.lastname: Subramaniam
authors.affiliation: NAFLD Translational Research Unit, University of California, San Diego, La Jolla, California, USA Division of Gastroenterology, Department of Medicine, University of California, San Diego, La Jolla, California, USA Division of Epidemiology, Department of Family Medicine and Public Health, University of California, San Diego, La Jolla, California, USA.
authors.forename: Rohit
authors.initials: R
authors.lastname: Loomba
mesh_terms: D002140:California / Q000453:epidemiology; D003430:Cross-Sectional Studies; D044127:Epigenesis, Genetic; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D008099:Liver / Q000000981:diagnostic imaging; D008279:Magnetic Resonance Imaging / Q000379:methods; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics; D008875:Middle Aged; D065626:Non-alcoholic Fatty Liver Disease / Q000097:blood / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics; D015995:Prevalence; D011446:Prospective Studies; D014429:Twins, Dizygotic / Q000235:genetics; D014430:Twins, Monozygotic / Q000235:genetics
chemical_list: C546086:MIRN30b microRNA, human; C544460:MIRN331 microRNA, human; D035683:MicroRNAs
keywords: FATTY LIVER; GENETICS; LIVER; NONALCOHOLIC STEATOHEPATITIS; OBESITY