title: Synergistic Potentials of Coffee on Injured Pancreatic Islets and Insulin Action via KATP Channel Blocking in Zebrafish.
abstract: Pancreatic islets (PIs) are damaged under diabetic conditions, resulting in decreased PI size. This study examined the regenerative effects of coffee and its components (caffeine, CFI; trigonelline, TRG; chlorogenic acid, CGA) on zebrafish larval PIs and β-cells damaged by administration of alloxan (AX). In addition, the influence of coffee and its active components on KATP channels was investigated using diazoxide (DZ) as a KATP channel activator. PI size and fluorescence intensity were significantly increased in the coffee-treated group relative to the no-treatment group (P < 0.0001). In addition, coffee exerted significant regenerative effects on pancreatic β-cells (p = 0.006). Treatment with TRG and CGA rescued PI damage, and the combination of TRG/CGA had a synergistic effect. In conclusion, the results indicate that coffee has beneficial effects on AX-damaged PIs and may also be useful as a blocker of pancreatic β-cell K(+) channels.
authors.forename: Youn Hee
authors.initials: YH
authors.lastname: Nam
authors.affiliation: §Department of Audiology, Nambu University, Gwangju 506-824, Republic of Korea.
authors.forename: Bin Na
authors.initials: BN
authors.lastname: Hong
authors.forename: Isabel
authors.initials: I
authors.lastname: Rodriguez
authors.forename: Min Gun
authors.initials: MG
authors.lastname: Ji
authors.forename: Keonwoo
authors.initials: K
authors.lastname: Kim
authors.forename: Ung-Jin
authors.initials: UJ
authors.lastname: Kim
authors.forename: Tong Ho
authors.initials: TH
authors.lastname: Kang
mesh_terms: D000470:Alkaloids / Q000494:pharmacology; D000496:Alloxan / Q000009:adverse effects; D000818:Animals; D002110:Caffeine / Q000494:pharmacology; D002726:Chlorogenic Acid / Q000494:pharmacology; D040503:Coffea / Q000737:chemistry*; D003069:Coffee / Q000737:chemistry*; D004357:Drug Synergism; D007328:Insulin / Q000378:metabolism*; D007515:Islets of Langerhans / Q000187:drug effects* / Q000293:injuries / Q000378:metabolism; D054086:KATP Channels / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D015027:Zebrafish
chemical_list: D000470:Alkaloids; D003069:Coffee; D007328:Insulin; D054086:KATP Channels; D010936:Plant Extracts; D002726:Chlorogenic Acid; D002110:Caffeine; C009560:trigonelline; D000496:Alloxan
keywords: chlorogenic acid; coffee; pancreatic islet; trigonelline; zebrafish larvae; β-cell