title: Regulation of Jasmonate-Mediated Stamen Development and Seed Production by a bHLH-MYB Complex in Arabidopsis.
abstract: Stamens are the plant male reproductive organs essential for plant fertility. Proper development of stamens is modulated by environmental cues and endogenous hormone signals. Deficiencies in biosynthesis or perception of the phytohormone jasmonate (JA) attenuate stamen development, disrupt male fertility, and abolish seed production in Arabidopsis thaliana. This study revealed that JA-mediated stamen development and seed production are regulated by a bHLH-MYB complex. The IIIe basic helix-loop-helix (bHLH) transcription factor MYC5 acts as a target of JAZ repressors to function redundantly with other IIIe bHLH factors such as MYC2, MYC3, and MYC4 in the regulation of stamen development and seed production. The myc2 myc3 myc4 myc5 quadruple mutant exhibits obvious defects in stamen development and significant reduction in seed production. Moreover, these IIIe bHLH factors interact with the MYB transcription factors MYB21 and MYB24 to form a bHLH-MYB transcription complex and cooperatively regulate stamen development. We speculate that the JAZ proteins repress the bHLH-MYB complex to suppress stamen development and seed production, while JA induces JAZ degradation and releases the bHLH-MYB complex to subsequently activate the expression of downstream genes essential for stamen development and seed production.
authors.affiliation: Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.
authors.forename: Tiancong
authors.initials: T
authors.lastname: Qi
authors.affiliation: Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.
authors.forename: Huang
authors.initials: H
authors.lastname: Huang
authors.affiliation: Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.
authors.forename: Susheng
authors.initials: S
authors.lastname: Song
authors.affiliation: Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China daoxinlab@tsinghua.edu.cn.
authors.forename: Daoxin
authors.initials: D
authors.lastname: Xie
mesh_terms: D017360:Arabidopsis / Q000254:growth & development* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000502:physiology*; D051792:Basic Helix-Loop-Helix Proteins / Q000502:physiology*; D003517:Cyclopentanes / Q000378:metabolism*; D035264:Flowers / Q000254:growth & development*; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D054883:Oxylipins / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000502:physiology*; D012639:Seeds / Q000254:growth & development*; D015534:Trans-Activators / Q000502:physiology; D014157:Transcription Factors / Q000502:physiology*
chemical_list: D029681:Arabidopsis Proteins; D051792:Basic Helix-Loop-Helix Proteins; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D015534:Trans-Activators; D014157:Transcription Factors; C473612:MYC3 protein, Arabidopsis; C000603837:MYC5 protein, Arabidopsis; C500589:Myb protein, Arabidopsis; C011006:jasmonic acid