title: A general method for synthesizing enzyme-polymer conjugates in reverse emulsions using Pluronic as a reactive surfactant.
abstract: Using aldehyde-functionalized Pluronic as the reactive surfactant, enzyme-Pluronic conjugates with sizes ranging from nanometers to micrometers were synthesized in reverse emulsions. Compared with the direct conjugation in aqueous solution, this method gave an increased conjugation efficiency and well-controlled size of the conjugates. The versatility of this method was validated using horseradish peroxidase (HRP), Candida rugosa lipase (CRL) and Candida antarctica lipase B (CALB). The resulting enzyme-Pluronic conjugates showed greatly enhanced apparent activity compared to free enzymes in organic media.
authors.affiliation: Key Lab for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China. junge@mail.tsinghua.edu.cn.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Wu
authors.forename: Jun
authors.initials: J
authors.lastname: Ge
authors.forename: Jingying
authors.initials: J
authors.lastname: Zhu
authors.forename: Yifei
authors.initials: Y
authors.lastname: Zhang
authors.forename: You
authors.initials: Y
authors.lastname: Yong
authors.forename: Zheng
authors.initials: Z
authors.lastname: Liu
mesh_terms: D002175:Candida / Q000201:enzymology*; D004655:Emulsions; D004800:Enzymes, Immobilized / Q000138:chemical synthesis*; D005656:Fungal Proteins / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*; D020442:Poloxamer / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D004655:Emulsions; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D011108:Polymers; D013501:Surface-Active Agents; D014867:Water; D020442:Poloxamer; D006735:Horseradish Peroxidase; D008049:Lipase; C431349:lipase B, Candida antarctica