title: Isolation and metagenomic characterization of bacteria associated with calcium carbonate and struvite precipitation in a pure moving bed biofilm reactor-membrane bioreactor.
abstract: A bench-scale pure moving bed bioreactor-membrane bioreactor (MBBR-MBR) used for the treatment of urban wastewater was analyzed for the identification of bacterial strains with the potential capacity for calcium carbonate and struvite biomineral formation. Isolation of mineral-forming strains on calcium carbonate and struvite media revealed six major colonies with a carbonate or struvite precipitation capacity in the biofouling on the membrane surface and showed that heterotrophic bacteria with the ability to precipitate calcium carbonate and struvite constituted ~7.5% of the total platable bacteria. These belonged to the genera Lysinibacillus, Trichococcus, Comamomas and Bacillus. Pyrosequencing analysis of the microbial communities in the suspended cells and membrane biofouling showed a high degree of similarity in all the samples collected with respect to bacterial assemblage. The study of operational taxonomic units (OTUs) identified through pyrosequencing suggested that ~21% of the total bacterial community identified in the biofouling could potentially form calcium carbonate or struvite crystals in the pure MBBR-MBR system used for the treatment of urban wastewater.
authors.affiliation: a Department of Civil Engineering , University of Granada , Granada , Spain.
authors.forename: A
authors.initials: A
authors.lastname: Gonzalez-Martinez
authors.forename: J C
authors.initials: JC
authors.lastname: Leyva-Díaz
authors.forename: A
authors.initials: A
authors.lastname: Rodriguez-Sanchez
authors.forename: B
authors.initials: B
authors.lastname: Muñoz-Palazon
authors.forename: A
authors.initials: A
authors.lastname: Rivadeneyra
authors.forename: J M
authors.initials: JM
authors.lastname: Poyatos
authors.forename: M A
authors.initials: MA
authors.lastname: Rivadeneyra
authors.forename: M V
authors.initials: MV
authors.lastname: Martinez-Toledo
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D018441:Biofilms / Q000254:growth & development; D057219:Biofouling; D019149:Bioreactors / Q000382:microbiology*; D002119:Calcium Carbonate / Q000737:chemistry*; D017616:Magnesium Compounds / Q000737:chemistry*; D008567:Membranes, Artificial; D056186:Metagenomics; D010710:Phosphates / Q000737:chemistry*; D000069877:Struvite; D062065:Wastewater / Q000737:chemistry / Q000382:microbiology
chemical_list: D002119:Calcium Carbonate; D017616:Magnesium Compounds; D008567:Membranes, Artificial; D010710:Phosphates; D000069877:Struvite; D062065:Wastewater
keywords: biofouling; biomineralization; calcium carbonate; metagenomics; pyrosequencing; struvite