title: Elucidating the relationship between superoxide anion levels and lifespan using an enhanced two-photon fluorescence imaging probe.
abstract: We report a new reversible fluorescent two-photon (TP) probe (PY-CA) with high TP absorption cross section and pH-independent fluorescence response, which allow monitoring of O2˙(-) fluxes dynamically, selectively and sensitively. The imaging results indicate that O2˙(-) at high levels can shorten the life of Caenorhabditis elegans.
authors.affiliation: College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, P. R. China. tangb@sdnu.edu.cn.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Fang
authors.initials: F
authors.lastname: Huang
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Bo
authors.initials: B
authors.lastname: Tang
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000254:growth & development* / Q000528:radiation effects; D005456:Fluorescent Dyes / Q000737:chemistry*; D008136:Longevity; D008856:Microscopy, Fluorescence; D061848:Optical Imaging / Q000379:methods*; D017785:Photons; D013481:Superoxides / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D013481:Superoxides