title: miRNA-449a is downregulated in osteosarcoma and promotes cell apoptosis by targeting BCL2.
abstract: Accumulating evidence reveals that miR-449a is expressed at a low level in several tumors and cancer cell lines, and acts as a tumor suppressor in several cancers. However, its role in osteosarcoma (OS) is not well understood. In the present study, we found that miR-449a was significantly downregulated in both OS tissues and cell lines. Furthermore, low expression level of miR-449a was correlated with advanced tumor stage, metastasis, and predicted a poor overall survival in OS patients. Additionally, restoration of miR-449a in OS cell lines U2OS and Saos-2 reduced cell viability, promoted cell apoptosis in vitro, and suppressed tumorigenicity in vivo. Moreover, BCL2, an antiapoptotic molecule, was identified to be a direct target of miR-449a, and the proapoptotic function of miR-449a was mainly through targeting BCL2 expression. Taken together, our results demonstrated a tumor-suppressive role of miR-449a in OS progression and suggested a potential therapeutic target for OS.
authors.affiliation: Department of Orthopaedic Surgery, Second Affiliated Hospital of Medical School of Xi'an Jiaotong University, No. 157 Xiwu Road, Xi'an, Shaanxi, 710004, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Orthopaedic Surgery, Second Affiliated Hospital of Medical School of Xi'an Jiaotong University, No. 157 Xiwu Road, Xi'an, Shaanxi, 710004, People's Republic of China.|Department of Orthopaedic Surgery, Xi'an No. 5 Hospital, Xi'an, Shaanxi, 710004, People's Republic of China.
authors.forename: Jinsong
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Orthopaedic Surgery, Second Affiliated Hospital of Medical School of Xi'an Jiaotong University, No. 157 Xiwu Road, Xi'an, Shaanxi, 710004, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Orthopaedic Surgery, Second Affiliated Hospital of Medical School of Xi'an Jiaotong University, No. 157 Xiwu Road, Xi'an, Shaanxi, 710004, People's Republic of China.
authors.forename: Baohui
authors.initials: B
authors.lastname: Yang
authors.affiliation: Department of Orthopaedic Surgery, Second Affiliated Hospital of Medical School of Xi'an Jiaotong University, No. 157 Xiwu Road, Xi'an, Shaanxi, 710004, People's Republic of China.
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Orthopaedic Surgery, Second Affiliated Hospital of Medical School of Xi'an Jiaotong University, No. 157 Xiwu Road, Xi'an, Shaanxi, 710004, People's Republic of China.
authors.forename: Pinglin
authors.initials: P
authors.lastname: Yang
authors.affiliation: Department of Orthopaedic Surgery, Second Affiliated Hospital of Medical School of Xi'an Jiaotong University, No. 157 Xiwu Road, Xi'an, Shaanxi, 710004, People's Republic of China.
authors.forename: Xijing
authors.initials: X
authors.lastname: He
authors.affiliation: Department of Orthopaedic Surgery, Second Affiliated Hospital of Medical School of Xi'an Jiaotong University, No. 157 Xiwu Road, Xi'an, Shaanxi, 710004, People's Republic of China. hpengli305@126.com.
authors.forename: Haopeng
authors.initials: H
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D001859:Bone Neoplasms / Q000235:genetics / Q000401:mortality / Q000473:pathology*; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D009367:Neoplasm Staging; D012516:Osteosarcoma / Q000235:genetics / Q000401:mortality / Q000556:secondary*; D011379:Prognosis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015996:Survival Rate; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays; D055815:Young Adult
chemical_list: C000595707:BCL2 protein, human; C544530:MIRN449 microRNA, human; D035683:MicroRNAs; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger
keywords: Apoptosis; BCL2; Osteosarcoma; Tumor suppressor; miR-449a