title: Flow-injection amperometric determination of glucose using a biosensor based on immobilization of glucose oxidase onto Au seeds decorated on core Fe₃O₄ nanoparticles.
abstract: An amperometric biosensor based on chemisorption of glucose oxidase (GOx) on Au seeds decorated on magnetic core Fe3O4 nanoparticles (Fe3O4@Au) and their immobilization on screen-printed carbon electrode bulk-modified with manganese oxide (SPCE{MnO2}) was designed for the determination of glucose. The Fe3O4@Au/GOx modified SPCE{MnO2} was used in a flow-injection analysis (FIA) arrangement. The experimental conditions were investigated in amperometric mode with the following optimized parameters: flow rate 1.7 mL min(-1), applied potential +0.38 V, phosphate buffer solution (PBS; 0.1 mol L(-1), pH 7.0) as carrier and 3.89 unit mm(-2) enzyme glucose oxidase loading on the active surface of the SPCE. The designed biosensor in FIA arrangement yielded a linear dynamic range for glucose from 0.2 to 9.0 mmol L(-1) with a sensitivity of 2.52 µA mM(-1) cm(-2), a detection limit of 0.1 mmol L(-1) and a quantification limit of 0.3 mmol L(-1). Moreover, a good repeatability of 2.8% (number of measurements n=10) and a sufficient reproducibility of 4.0% (number of sensors n=3) were achieved. It was found that the studied system Fe3O4@Au facilitated not only a simpler enzyme immobilization but also provided wider linear range. The practical application of the proposed biosensor for FIA quantification of glucose was tested in glucose sirup samples, honeys and energy drinks with the results in good accordance with those obtained by an optical glucose meter and with the contents declared by the producers.
authors.affiliation: Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, UbonRatchathani University, UbonRatchathani 34190, Thailand; Department of Chemistry, Faculty of Science, UbonRatchathani University, UbonRatchathani 34190, Thailand. Electronic address: scanchsa@mail2.ubu.ac.th.
authors.forename: Anchalee
authors.initials: A
authors.lastname: Samphao
authors.affiliation: Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, UbonRatchathani University, UbonRatchathani 34190, Thailand.
authors.forename: Preeyanut
authors.initials: P
authors.lastname: Butmee
authors.affiliation: Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, UbonRatchathani University, UbonRatchathani 34190, Thailand.
authors.forename: Juthamas
authors.initials: J
authors.lastname: Jitcharoen
authors.affiliation: Institute of Analytical Chemistry, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, Bratislava SK-812 37, Slovak Republic.
authors.forename: Ľubomír
authors.initials: Ľ
authors.lastname: Švorc
authors.affiliation: Institute of Chemistry-Analytical Chemistry, Karl-Franzens University, A-8010 Graz, Austria.
authors.forename: Georg
authors.initials: G
authors.lastname: Raber
authors.affiliation: Institute of Chemistry-Analytical Chemistry, Karl-Franzens University, A-8010 Graz, Austria.
authors.forename: Kurt
authors.initials: K
authors.lastname: Kalcher
mesh_terms: D015374:Biosensing Techniques; D004563:Electrochemistry; D061215:Energy Drinks / Q000032:analysis; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006722:Honey / Q000032:analysis; D006861:Hydrogen Peroxide / Q000737:chemistry; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D017895:Manganese Compounds / Q000737:chemistry; D010087:Oxides / Q000737:chemistry
chemical_list: D004800:Enzymes, Immobilized; D058185:Magnetite Nanoparticles; D017895:Manganese Compounds; D010087:Oxides; C027424:manganese oxide; D006046:Gold; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005947:Glucose
keywords: Amperometric biosensor; Core–shell magnetite gold nanoparticles; Flow-injection analysis; Glucose; Screen-printed electrode