title: Age-related changes in mitochondrial antioxidant enzyme Trx2 and TXNIP-Trx2-ASK1 signal pathways in the auditory cortex of a mimetic aging rat model: changes to Trx2 in the auditory cortex.
abstract: Age-associated degeneration in the central auditory system, which is defined as central presbycusis, can impair sound localization and speech perception. Research has shown that oxidative stress plays a central role in the pathological process of central presbycusis. Thioredoxin 2 (Trx2), one member of thioredoxin family, plays a key role in regulating the homeostasis of cellular reactive oxygen species and anti-apoptosis. The purpose of this study was to explore the association between Trx2 and the phenotype of central presbycusis using a mimetic aging animal model induced by long-term exposure to d-galactose (d-Gal). We also explored changes in thioredoxin-interacting protein (TXNIP), apoptosis signal regulating kinase 1 (ASK1) and phosphorylated ASK1 (p-ASK1) expression, as well as the Trx2-TXNIP/Trx2-ASK1 binding complex in the auditory cortex of mimetic aging rats. Our results demonstrate that, compared with control groups, the levels of Trx2 and Trx2-ASK1 binding complex were significantly reduced, whereas TXNIP, ASK1 p-ASK1 expression, and Trx2-TXNIP binding complex were significantly increased in the auditory cortex of the mimetic aging groups. Our results indicated that changes in Trx2 and the TXNIP-Trx2-ASK1 signal pathway may participate in the pathogenesis of central presbycusis.
authors.affiliation: Department of Otorhinolaryngology, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Hai-Ying
authors.initials: HY
authors.lastname: Sun
authors.affiliation: Department of Otorhinolaryngology, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Yu-Juan
authors.initials: YJ
authors.lastname: Hu
authors.affiliation: Department of Otorhinolaryngology, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Xue-Yan
authors.initials: XY
authors.lastname: Zhao
authors.affiliation: Department of Otorhinolaryngology, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhong
authors.affiliation: Department of Otorhinolaryngology, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: Zeng
authors.affiliation: Department of Otorhinolaryngology, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Xu-Bo
authors.initials: XB
authors.lastname: Chen
authors.affiliation: Department of Otorhinolaryngology, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Department of Otorhinolaryngology, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Otorhinolaryngology, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Endocrinology, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Kong
authors.affiliation: Department of Otorhinolaryngology, Huazhong University of Science and Technology, Wuhan, China.|Key Laboratory of Neurological Disease, Ministry of Education, Wuhan, China.
authors.forename: Wei-Jia
authors.initials: WJ
authors.lastname: Kong
mesh_terms: D000375:Aging; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000235:genetics; D001303:Auditory Cortex / Q000166:cytology / Q000378:metabolism* / Q000502:physiology / Q000648:ultrastructure; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins; D005786:Gene Expression Regulation; D048848:MAP Kinase Kinase Kinase 5 / Q000235:genetics / Q000378:metabolism*; D008315:Malondialdehyde / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D018384:Oxidative Stress; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D013879:Thioredoxins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; C493796:TXNIP protein, rat; C516113:Txn2 protein, rat; D008315:Malondialdehyde; D013879:Thioredoxins; D013482:Superoxide Dismutase; D048848:MAP Kinase Kinase Kinase 5; C482858:MAP3K5 protein, human
keywords: age-related hearing loss; apoptosis; auditory cortex; oxidant stress; thioredoxin 2