title: miR-582-5p inhibits proliferation of hepatocellular carcinoma by targeting CDK1 and AKT3.
abstract: microRNAs play an important role in the progression of hepatocellular carcinoma (HCC). In this study, we found that miR-582-5p expression was downregulated in hepatoma tissues and HCC cell lines. Upregulation of miR-582-5p reduced colony number, inhibited cellular proliferation, and arrested cell cycle in G0/G1 phase. When miR-582-5p was inhibited, the colony number was increased and cellular proliferation and cell cycle were promoted. Further studies showed that miR-582-5p regulated the progression of HCC through directly inhibiting the expression of CDK1 and AKT3, and indirectly inhibiting the expression of cyclinD1.
authors.affiliation: Zhongnan Hospital of Wuhan University, Institute of Hepatobiliary Diseases of Wuhan University, Transplant Center of Wuhan University, Hubei Key Laboratory of Medical Technology on Transplantation, Wuhan, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Oncology Department of the First People's Hospital of Shunde, Guangzhou, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.affiliation: Anesthesia Department, Zhongnan Hospital of Wuhan University, Wuhan, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Ran
authors.affiliation: Zhongnan Hospital of Wuhan University, Institute of Hepatobiliary Diseases of Wuhan University, Transplant Center of Wuhan University, Hubei Key Laboratory of Medical Technology on Transplantation, Wuhan, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: Zhongnan Hospital of Wuhan University, Institute of Hepatobiliary Diseases of Wuhan University, Transplant Center of Wuhan University, Hubei Key Laboratory of Medical Technology on Transplantation, Wuhan, China.
authors.forename: Zibiao
authors.initials: Z
authors.lastname: Zhong
authors.affiliation: Zhongnan Hospital of Wuhan University, Institute of Hepatobiliary Diseases of Wuhan University, Transplant Center of Wuhan University, Hubei Key Laboratory of Medical Technology on Transplantation, Wuhan, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Fan
authors.affiliation: Zhongnan Hospital of Wuhan University, Institute of Hepatobiliary Diseases of Wuhan University, Transplant Center of Wuhan University, Hubei Key Laboratory of Medical Technology on Transplantation, Wuhan, China.
authors.forename: Zhenghua
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Zhongnan Hospital of Wuhan University, Institute of Hepatobiliary Diseases of Wuhan University, Transplant Center of Wuhan University, Hubei Key Laboratory of Medical Technology on Transplantation, Wuhan, China. yqf_china@163.com.|Research Center of National Health Ministry on Transplantation Medicine Engineering and Technology, The 3rd Xiangya Hospital of Central South University, Changsha, China. yqf_china@163.com.|Zhongnan Hospital of Wuhan University, Donghu Road 169#, Wuchang District, Wuhan, 430071, China. yqf_china@163.com.
authors.forename: Qifa
authors.initials: Q
authors.lastname: Ye
mesh_terms: D016203:CDC2 Protein Kinase; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D018844:Cyclin-Dependent Kinases / Q000096:biosynthesis* / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics*; D051057:Proto-Oncogene Proteins c-akt / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C000593706:MIRN582 microRNA, human; D035683:MicroRNAs; C491754:AKT3 protein, human; D051057:Proto-Oncogene Proteins c-akt; D016203:CDC2 Protein Kinase; C528899:CDK1 protein, human; D018844:Cyclin-Dependent Kinases
keywords: AKT3; CDK1; CyclinD1; Hepatocellular carcinoma; miR-582-5p