title: Molecular cloning, characterization and expression analysis of cathepsin O in silkworm Bombyx mori related to bacterial response.
abstract: Cathepsins are the main members of the cysteine family and play important roles in immune response in vertebrates. The Cathepsin O of Bombyx mori (BmCathepsin O) was cloned from the hemocytes by the rapid amplification of cDNA ends (RACE). The genomic DNA was 6131bp long with a total of six exons and five introns. Its pre-mRNA was spliced to generate two spliceosomes. By comparisons with other reported cathepsins O, it was concluded that the identity between them ranged from 29 to 39%. Expression analysis indicated that BmCathepsin O was specific-expressed in hemocytes, and highly expressed at the 4th molting and metamorphosis stages. Immunofluorescence assay and qRT-PCR showed that BmCathepsin O was expressed in granulocytes and plasmatocytes. Interestingly, BmCathepsin O was significantly up-regulated after stimulated by 20-hydroxyecdysone (20-E) in vivo, which suggested that BmCathepsin O may be regulated by 20E. Moreover, activation of BmCathepsin O was also observed in hemocytes challenged by Escherichia coli, indicating its potential involvement in the innate immune system of silkworm, B. mori. In summary, our studies provide a new insight into the functional features of Cathepsin O.
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, The Institute of Sericulture and Systems Biology, Southwest University, Chongqing 400716, China.
authors.forename: Kui
authors.initials: K
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, The Institute of Sericulture and Systems Biology, Southwest University, Chongqing 400716, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Su
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, The Institute of Sericulture and Systems Biology, Southwest University, Chongqing 400716, China.
authors.forename: Siyuan
authors.initials: S
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, The Institute of Sericulture and Systems Biology, Southwest University, Chongqing 400716, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, The Institute of Sericulture and Systems Biology, Southwest University, Chongqing 400716, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Tan
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, The Institute of Sericulture and Systems Biology, Southwest University, Chongqing 400716, China.
authors.forename: Man
authors.initials: M
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, The Institute of Sericulture and Systems Biology, Southwest University, Chongqing 400716, China.
authors.forename: Hanghua
authors.initials: H
authors.lastname: Liang
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, The Institute of Sericulture and Systems Biology, Southwest University, Chongqing 400716, China.
authors.forename: Yuzu
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, The Institute of Sericulture and Systems Biology, Southwest University, Chongqing 400716, China.
authors.forename: Huijuan
authors.initials: H
authors.lastname: Chao
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, The Institute of Sericulture and Systems Biology, Southwest University, Chongqing 400716, China.
authors.forename: Liqun
authors.initials: L
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, The Institute of Sericulture and Systems Biology, Southwest University, Chongqing 400716, China. Electronic address: hongjuan.cui@gmail.com.
authors.forename: Hongjuan
authors.initials: H
authors.lastname: Cui
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000276:immunology*; D002403:Cathepsins / Q000235:genetics / Q000276:immunology*; D003001:Cloning, Molecular; D004441:Ecdysterone / Q000494:pharmacology; D004926:Escherichia coli / Q000276:immunology; D005091:Exons; D015870:Gene Expression; D006434:Hemocytes / Q000276:immunology* / Q000382:microbiology; D007113:Immunity, Innate; D019476:Insect Proteins / Q000235:genetics / Q000276:immunology*; D007438:Introns; D008675:Metamorphosis, Biological / Q000276:immunology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D019476:Insect Proteins; D011994:Recombinant Proteins; D004441:Ecdysterone; D002403:Cathepsins
keywords: 20-Ecdysone; Cathepsin O; Infection; Molecular cloning; Silkworm