title: Wastewater Analysis Indicates that Genetically Diverse Astroviruses, Including Strains Belonging to Novel Clades MLB and VA, Are Circulating within Japanese Populations.
abstract: Human astroviruses (HAstVs) are a common etiological agent of infantile gastroenteritis. Recent studies revealed that novel astrovirus (AstV) strains of the MLB clade (MLB-AstVs) and VA clade (VA-AstVs), which are genetically distinct from the classic HAstVs, are circulating in the human population. In the present study, we quantified classic HAstVs as well as carried out a genetic analysis of classic and novel HAstVs in wastewater in Japan. The concentration of classic HAstVs in the influent water samples ranged from 10(4) to 10(5) copies per liter, and the amount removed by wastewater treatment was determined to be 2.4 ± 0.3 log10. Four types of classic HAstV strains (HAstV types 1, 2, 5, and 4/8) as well as novel AstV strains belonging to the MLB-2, VA-1, and VA-2 clades were identified using reverse transcription-PCR (RT-PCR) assays, including assays newly developed for the detection of strains of the MLB and VA clades, followed by cloning and nucleotide sequencing. Our results suggest that genetically diverse AstV strains are circulating among the human population in Japan. The newly developed (semi)nested RT-PCR assays for these novel AstV clades are useful to identify and characterize the novel AstVs in environmental waters.
authors.affiliation: Research Center for Environmental Quality Management, Kyoto University, Shiga, Japan hata.akihiko.33c@st.kyoto-u.ac.jp.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Hata
authors.affiliation: Department of Urban Engineering, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Katayama
authors.affiliation: Center for Environmental Sensing and Modeling, Singapore-MIT Alliance for Research and Technology, Singapore, Singapore.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Kitajima
authors.affiliation: Department of Urban Engineering, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Furumai
mesh_terms: D019350:Astroviridae Infections / Q000453:epidemiology* / Q000821:virology*; D006801:Humans; D007564:Japan / Q000453:epidemiology; D001257:Mamastrovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D019562:Viral Load; D062065:Wastewater / Q000821:virology*
chemical_list: D012367:RNA, Viral; D062065:Wastewater