title: Distance measurements between manganese(II) and nitroxide spin-labels by DEER determine a binding site of Mn(2+) in the HP92 loop of ribosomal RNA.
abstract: Mn(2+) localization in hairpin 92 of the 23S ribosomal RNA (HP92) was obtained using W-band (95 GHz) DEER (double electron-electron resonance) distance measurements between the Mn(2+) ion and nitroxide spin labels on the RNA. It was found to be preferably situated in the minor groove of the double strand region close to the HP92 loop.
authors.affiliation: Department of Chemical Physics, Weizmann Institute of Science, Rehovot 76100, Israel.
authors.forename: Ilia
authors.initials: I
authors.lastname: Kaminker
authors.forename: Morgan
authors.initials: M
authors.lastname: Bye
authors.forename: Natanel
authors.initials: N
authors.lastname: Mendelman
authors.forename: Kristmann
authors.initials: K
authors.lastname: Gislason
authors.forename: Snorri Th
authors.initials: ST
authors.lastname: Sigurdsson
authors.forename: Daniella
authors.initials: D
authors.lastname: Goldfarb
mesh_terms: D001665:Binding Sites; D004578:Electron Spin Resonance Spectroscopy; D006860:Hydrogen Bonding; D008345:Manganese / Q000737:chemistry*; D009589:Nitrogen Oxides / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D012338:RNA, Ribosomal, 23S / Q000737:chemistry*; D013113:Spin Labels
chemical_list: D009589:Nitrogen Oxides; D012338:RNA, Ribosomal, 23S; D013113:Spin Labels; D008345:Manganese; C039900:nitroxyl