title: Integration of nondegradable polystyrene and degradable gelatin in a core-sheath nanofibrous patch for pelvic reconstruction.
abstract: Pelvic organ prolapse (POP) is a serious health issue affecting many adult women. Complications of POP include pelvic pressure, pelvic pain, and problems in emptying their bowels or bladder. Sometimes, POP may even cause urinary outflow obstruction and lead to bladder or kidney infections. Currently, synthetic and naturally derived materials have been chosen for treatment of POP to reduce the high recurrence rates after surgical interventions. However, existing materials for POP treatment cannot meet the clinical requirements in terms of biocompatibility, mechanics, and minimal risk of rejection. Especially, erosion in synthetic polymers and rapid degradation in natural polymers limit their further applications in clinics. To address these concerns, we report a novel POP replacement using core-sheath polystyrene/gelatin electrospun nanofiber mesh. The outside gelatin sheath provides a hydrophilic surface and implantable integrity between host and guest, while the inner PS core offers the necessary mechanical support. The composite mesh shows graft accommodation in pelvic submucosa after implantation in vivo, as shown in hematoxylin-eosin staining and T helper cell phenotype and macrophage phenotype stainings. Qualitative analysis of inducible nitric oxide synthase, arginase, interferon-γ, and interleukin-10 gene expressions also indicates that the implanted composite mesh switches to accommodation mode 2 weeks postimplantation. Thus, these novel core-sheath polystyrene/gelatin nanofibrous membranes are promising in pelvic reconstruction.
authors.affiliation: Chongqing Academy of Animal Sciences, Chongqing, People's Republic of China ; Department of Mechanical and Manufacturing Engineering, Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, MB, Canada ; Manitoba Institute of Child Health, Winnipeg, MB, Canada ; Key Laboratory of Pig Industry Sciences, Ministry of Agriculture,Chongqing, People's Republic of China ; Chongqing Key Laboratory of Pig Industry Sciences, Chongqing, People's Republic of China.
authors.forename: Liangpeng
authors.initials: L
authors.lastname: Ge
authors.affiliation: Department of Mechanical and Manufacturing Engineering, Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, MB, Canada ; Manitoba Institute of Child Health, Winnipeg, MB, Canada.
authors.forename: Qingtao
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Mechanical and Manufacturing Engineering, Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, MB, Canada ; Manitoba Institute of Child Health, Winnipeg, MB, Canada.
authors.forename: Junzi
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Chongqing Academy of Animal Sciences, Chongqing, People's Republic of China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: You
authors.affiliation: Chongqing Academy of Animal Sciences, Chongqing, People's Republic of China.
authors.forename: Zuohua
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Textile Sciences, Faculty of Human Ecology, University of Manitoba, Winnipeg, MB, Canada.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhong
authors.affiliation: Chongqing Academy of Animal Sciences, Chongqing, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Mechanical and Manufacturing Engineering, Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, MB, Canada ; Manitoba Institute of Child Health, Winnipeg, MB, Canada.
authors.forename: Malcolm M Q
authors.initials: MM
authors.lastname: Xing
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D005780:Gelatin / Q000737:chemistry / Q000494:pharmacology; D008297:Male; D051379:Mice; D057139:Nanofibers / Q000737:chemistry*; D056887:Pelvic Organ Prolapse; D011137:Polystyrenes / Q000737:chemistry / Q000494:pharmacology; D017207:Rats, Sprague-Dawley; D019651:Plastic Surgery Procedures / Q000295:instrumentation*; D013526:Surgical Mesh; D014621:Vagina / Q000601:surgery
chemical_list: D001672:Biocompatible Materials; D011137:Polystyrenes; D005780:Gelatin
keywords: core-sheath nanofibrous patch; gelatin; pelvic reconstruction; polystyrene