title: Identification of fungal ene-reductase activity by means of a functional screening.
abstract: Bioeconomy stresses the need of green processes promoting the development of new methods for biocatalyzed alkene reductions. A functional screening of 28 fungi belonging to Ascomycota, Basidiomycota, and Zygomycota isolated from different habitats was performed to analyze their capability to reduce C=C double bonds towards three substrates (cyclohexenone, α-methylnitrostyrene, and α-methylcinnamaldehyde) with different electron-withdrawing groups, i.e., ketone, nitro, and aldehyde, respectively. Almost all the fungi showed this reducing activity. Noteworthy Gliomastix masseei, Mucor circinelloides, and Mucor plumbeus resulted versatile and effective, being able to reduce all the model substrates quickly and with high yields.
authors.affiliation: Department of Life Science and Systems Biology, University of Turin, Viale P.A. Mattioli 25, 10125 Turin, Italy. Electronic address: alice.romagnolo@unito.it.
authors.forename: Alice
authors.initials: A
authors.lastname: Romagnolo
authors.affiliation: Department of Life Science and Systems Biology, University of Turin, Viale P.A. Mattioli 25, 10125 Turin, Italy. Electronic address: federica.spina@unito.it.
authors.forename: Federica
authors.initials: F
authors.lastname: Spina
authors.affiliation: Department of Chemistry, Materials and Chemical Engineering "G. Natta", Politecnico di Milano, Via L. Mancinelli 7, 20131 Milan, Italy. Electronic address: elisabetta.brenna@polimi.it.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Brenna
authors.affiliation: Department of Chemistry, Materials and Chemical Engineering "G. Natta", Politecnico di Milano, Via L. Mancinelli 7, 20131 Milan, Italy. Electronic address: michele.crotti@chem.polimi.it.
authors.forename: Michele
authors.initials: M
authors.lastname: Crotti
authors.affiliation: Department of Chemistry, Materials and Chemical Engineering "G. Natta", Politecnico di Milano, Via L. Mancinelli 7, 20131 Milan, Italy.
authors.forename: Fabio
authors.initials: F
authors.lastname: Parmeggiani
authors.affiliation: Department of Life Science and Systems Biology, University of Turin, Viale P.A. Mattioli 25, 10125 Turin, Italy. Electronic address: cristina.varese@unito.it.
authors.forename: Giovanna Cristina
authors.initials: GC
authors.lastname: Varese
mesh_terms: D000475:Alkenes / Q000378:metabolism*; D001203:Ascomycota / Q000378:metabolism*; D001487:Basidiomycota / Q000378:metabolism*; D008403:Mass Screening; D009089:Mucor / Q000378:metabolism*; D010084:Oxidation-Reduction
chemical_list: D000475:Alkenes
keywords: Biocatalysis; Bioreduction; Carbon–carbon double bond; Filamentous fungi; α,β-unsaturated compounds