title: Electrophysiological and behavioral responses of Diaphania glauculalis males to female sex pheromone.
abstract: The aim of this study is to identify the pheromone active component of female moths, Diaphania glauculalis, an important pest of Anthocephalus chinensis in China. The sex pheromone was extracted from sex pheromone gland extracts of virgin female moth of D. glauculalis using n-hexane, and the pheromone gland extracts of females were analyzed using coupled gas chromatography-electroantennogram detection (GC-EAD) and gas chromatography-mass spectrometry (GC-MS). The sex pheromone active components were based on the comparison the retention time and mass spectrum, with suitable synthetic compounds. (E)-11-hexadecenal (E11-16:Ald) and (E,E)-10,12-hexadecadienal (E10E12-16:Ald) were identified as the major sex pheromone components in the females. Their biological activities were evaluated in a series of electroantennogram (EAG) experiments and four-arm olfactometer assays using synthetic compounds. D. glauculalis males could be attracted by any single component, but a mixture of the E11-16:Ald and E10E12-16:Ald in a ratio of 5:5 elicited a substantial response, demonstrating that the binary blend is essential in male attraction. We therefore conclude that the aldehyde compounds, a mixture of E11-16:Ald and E10E12-16:Ald, comprise the sex pheromone components of D. glauculalis, which might be applied for insect field trapping.
authors.affiliation: Research Centre for Health Diagnosis and Protection Techniques of Woody Plants, College of Forestry and Landscape Architecture, South China Agricultural University, No. 483, Tianhe District, Guangzhou, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Ma
authors.affiliation: Research Centre for Health Diagnosis and Protection Techniques of Woody Plants, College of Forestry and Landscape Architecture, South China Agricultural University, No. 483, Tianhe District, Guangzhou, China.
authors.forename: Zhi Tao
authors.initials: ZT
authors.lastname: Liu
authors.affiliation: Research Centre for Health Diagnosis and Protection Techniques of Woody Plants, College of Forestry and Landscape Architecture, South China Agricultural University, No. 483, Tianhe District, Guangzhou, China.
authors.forename: Yuan Yuan
authors.initials: YY
authors.lastname: Zhang
authors.affiliation: Research Centre for Health Diagnosis and Protection Techniques of Woody Plants, College of Forestry and Landscape Architecture, South China Agricultural University, No. 483, Tianhe District, Guangzhou, China.
authors.forename: Zhao Hui
authors.initials: ZH
authors.lastname: Sun
authors.affiliation: Research Centre for Health Diagnosis and Protection Techniques of Woody Plants, College of Forestry and Landscape Architecture, South China Agricultural University, No. 483, Tianhe District, Guangzhou, China.
authors.forename: Yi Zhen
authors.initials: YZ
authors.lastname: Li
authors.affiliation: Research Centre for Health Diagnosis and Protection Techniques of Woody Plants, College of Forestry and Landscape Architecture, South China Agricultural University, No. 483, Tianhe District, Guangzhou, China. wenxiujun@scau.edu.cn.
authors.forename: Xiu Jun
authors.initials: XJ
authors.lastname: Wen
authors.affiliation: Research Centre for Health Diagnosis and Protection Techniques of Woody Plants, College of Forestry and Landscape Architecture, South China Agricultural University, No. 483, Tianhe District, Guangzhou, China.
authors.forename: Xiao Yang
authors.initials: XY
authors.lastname: Chen
mesh_terms: D000447:Aldehydes / Q000302:isolation & purification / Q000494:pharmacology; D000466:Alkadienes / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D001681:Biological Assay; D002681:China; D002849:Chromatography, Gas; D055724:Electrophysiological Phenomena; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D013058:Mass Spectrometry; D009036:Moths / Q000502:physiology*; D012724:Sex Attractants / Q000737:chemistry* / Q000494:pharmacology* / Q000502:physiology; D012726:Sexual Behavior, Animal / Q000187:drug effects*
chemical_list: D000447:Aldehydes; D000466:Alkadienes; D012724:Sex Attractants; C061128:11-hexadecenal; C066482:bombykal
keywords: Diaphania glauculalis; Electroantennogram; Identification; Lepidoptera; Sex pheromone