title: Human mastadenovirus type 70: a novel, multiple recombinant species D mastadenovirus isolated from diarrhoeal faeces of a haematopoietic stem cell transplantation recipient.
abstract: A human mastadenovirus D (HAdV-D) isolated from diarrhoeal faeces of an allogeneic haematopoietic stem cell transplant (SCT) recipient was found to be non-typable by sequencing of loops 1 and 2 of the hexon main neutralization epitope ('imputed serology'). In contrast to HAdV-C, HAdV-D infections are rarely observed in SCT patients. Therefore, the whole genome of this isolate was sequenced and phylogenetically analysed. In addition, microneutralization testing with type-specific antisera was performed. A complete genomic sequence of 35.2 kb in length with a GC content of 57  % was obtained and found to be distantly related to HAdV-D27 (96.25 % identity). Imputed serology implicated a new type with a nucleotide sequence identity of only 96.11 % to HAdV-D37 (loop 1) and 95.76 % to HAdV-D30 and HAdV-D37 (loop 2). Microneutralization testing confirmed that this clinical isolate was not neutralized by HAdV-D37- or HAdV-D30-specific antisera. The penton base gene showed a novel sequence, which clustered with HAdV-D38, but bootscan analysis indicated an intra-penton recombination event with HAdV-D60. Another recombination event was detected within the early gene region E3 with the 12.2 kDa and CR1-α genes derived from HAdV-D58. Moreover, the E4 region was derived from HAdV-D13, but all these genes had evolved significantly from their ancestors. By contrast, the recombinant fibre gene was almost 100 % identical to HAdV-D29. In conclusion, the genomics of this novel HAdV, designated the HAdV-D70 [P70H70F29] prototype, supported the significance of multiple recombinations in the phylogeny of HAdV-D.
authors.affiliation: Institut für Virologie, Adenovirus Konsiliarlabor, Medizinische Hochschule Hannover, Hannover, Germany.|Deutsches Zentrum Infektionsforschung, Hannover and Braunschweig, Germany.
authors.forename: Elias
authors.initials: E
authors.lastname: Hage
authors.affiliation: Institut für Virologie, Universität Leipzig, Leipzig, Germany.
authors.forename: Uwe
authors.initials: U
authors.lastname: Gerd Liebert
authors.affiliation: Institut für Virologie, Universität Leipzig, Leipzig, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Bergs
authors.affiliation: Institut für Virologie, Adenovirus Konsiliarlabor, Medizinische Hochschule Hannover, Hannover, Germany.|Deutsches Zentrum Infektionsforschung, Hannover and Braunschweig, Germany.
authors.forename: Tina
authors.initials: T
authors.lastname: Ganzenmueller
authors.affiliation: Deutsches Zentrum Infektionsforschung, Hannover and Braunschweig, Germany.|Institut für Virologie, Adenovirus Konsiliarlabor, Medizinische Hochschule Hannover, Hannover, Germany.
authors.forename: Albert
authors.initials: A
authors.lastname: Heim
mesh_terms: D000257:Adenoviridae Infections / Q000209:etiology / Q000821:virology*; D003967:Diarrhea / Q000821:virology*; D005243:Feces / Q000821:virology*; D016679:Genome, Viral; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D006801:Humans; D017921:Mastadenovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D008875:Middle Aged; D008969:Molecular Sequence Data; D010802:Phylogeny; D011183:Postoperative Complications / Q000821:virology*; D011995:Recombination, Genetic; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins