title: PCR-free shotgun sequencing of the stone loach mitochondrial genome (Barbatula barbatula).
abstract: The complete mitochondrial genome of the stone loach Barbatula barbatula (Linnaeus, 1758) (Actinopterygii: Cypriniformes: Nemacheilidae) has been sequenced using a genome-skimming approach on an Illumina Hiseq 2500 platform. The mitochondrial genome of B. barbatula was determined to be 16,630 bp long and presents an organization typical of vertebrate mitogenomes. The mean coverage was 82× with a minimum coverage of 33× for the control region and 52× for the remaining part of the genome. A phylogenetic analysis of the Nemacheilidae family shows the monophyly of the Barbatula genus with strong support.
authors.affiliation: a CNRS, Université de Toulouse III Paul Sabatier, ENFA, UMR5174 EDB (Laboratoire Evolution et Diversité Biologique) , Toulouse , France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Murienne
authors.affiliation: b INRA, UAR1209, Département de Génétique Animale , INRA Auzeville , Castanet-Tolosan , France.|c GeT-PlaGe, Genotoul, INRA Auzeville , Castanet-Tolosan , France.
authors.forename: Céline
authors.initials: C
authors.lastname: Jeziorski
authors.affiliation: a CNRS, Université de Toulouse III Paul Sabatier, ENFA, UMR5174 EDB (Laboratoire Evolution et Diversité Biologique) , Toulouse , France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Holota
authors.affiliation: d Laboratoire d'Ecologie Alpine , CNRS UMR , Grenoble , France , and.
authors.forename: Eric
authors.initials: E
authors.lastname: Coissac
authors.affiliation: a CNRS, Université de Toulouse III Paul Sabatier, ENFA, UMR5174 EDB (Laboratoire Evolution et Diversité Biologique) , Toulouse , France.|e Station Expérimentale du CNRS à Moulis, U.S.R , Moulis , France.
authors.forename: Simon
authors.initials: S
authors.lastname: Blanchet
authors.affiliation: a CNRS, Université de Toulouse III Paul Sabatier, ENFA, UMR5174 EDB (Laboratoire Evolution et Diversité Biologique) , Toulouse , France.
authors.forename: Gaël
authors.initials: G
authors.lastname: Grenouillet
mesh_terms: D000818:Animals; D003531:Cypriniformes / Q000235:genetics*; D054629:Genome, Mitochondrial; D010802:Phylogeny
keywords: Fish; genome skimming; illumina; next-generation sequencing