title: Protective immunity against Eimeria tenella infection in chickens following oral immunization with Bacillus subtilis expressing Eimeria tenella 3-1E protein.
abstract: The current experiment was conducted to construct recombinant Bacillus subtilis WB600 expressing Eimeria tenella 3-1E protein to investigate the oral immunization protective effects against E. tenella. The merozoite surface antigen 3-1E gene of E. tenella was introduced into the pBS-H1 expression vector with a novel signal peptide sequence. After the electro-transformation, the expression of objective protein in B. subtilis WB600 was detected by Western blot. The results showed that the recombinant B. subtilis strain with the ability of high-level secretion of 3-1E was constructed successfully. Seven-day-old broiler chickens were orally vaccinated with B. subtilis WB600 harboring 3-1E (B.S-pBS-H1-3-1E) or B. subtilis WB600 with empty plasmid (B.S-pBS-H1) 10 days prior to challenge with sporulated E. tenella oocysts. The results showed the recombinant B. subtilis strain with the ability of high-level secretion of 3-1E was constructed successfully. Vaccination with B.S-pBS-H1-3-1E strain significantly increased the anti-coccidial index and reduced cecal lesion scores compared with the positive control group (chickens were challenged with sporulated E. tenella oocysts without oral administration of B.S-pBS-H1-3-1E strain) and B.S-pBS-H1 group. Ceca mucosal sIgA, secretion, and IL-2, IL-12, IFN-γ, and IL-10 level after challenge were greater in the B.S-pBS-H1-3-1E group than in the positive control group. Taken together, these results indicated that B. subtilis WB600 harboring 3-1E protein induces protective immunity against E. tenella.
authors.affiliation: Key Laboratory of Molecular Animal Nutrition, Ministry of Education, College of Animal Sciences, Zhejiang University, 388 Yuhangtang Road, West Lake District, Hangzhou, 310058, People's Republic of China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Lin
authors.forename: Yanyun
authors.initials: Y
authors.lastname: Shi
authors.forename: Bin
authors.initials: B
authors.lastname: Deng
authors.forename: Xiangfei
authors.initials: X
authors.lastname: Mao
authors.forename: Dongyou
authors.initials: D
authors.lastname: Yu
authors.forename: Weifen
authors.initials: W
authors.lastname: Li
mesh_terms: D000818:Animals; D001412:Bacillus subtilis / Q000235:genetics; D001428:Bacterial Vaccines / Q000276:immunology*; D002645:Chickens / Q000276:immunology; D003048:Coccidiosis / Q000517:prevention & control / Q000662:veterinary*; D016786:Eimeria tenella / Q000378:metabolism*; D018664:Interleukin-12 / Q000235:genetics; D007376:Interleukin-2; D011201:Poultry Diseases / Q000517:prevention & control*; D015800:Protozoan Proteins / Q000276:immunology; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D014611:Vaccination / Q000379:methods
chemical_list: D001428:Bacterial Vaccines; D007376:Interleukin-2; D015800:Protozoan Proteins; D011994:Recombinant Proteins; D018664:Interleukin-12