title: [Association between BRAF V600E mutation and central lymph node metastasis in patients with papillary thyroid carcinoma].
abstract: OBJECTIVE
  To investigate the association of concomitant BRAFV600E mutation with central lymph node metastases in papillary thyroid carcinoma (PTC).
  
  
  METHODS
  The clinicopathological data of 126 PTC patients who underwent surgical treatment within a period of 2 years were retrospectively analyzed. The BRAF V600E gene mutation was detected by quantitative fluorescence PCR.
  
  
  RESULTS
  The BRAF mutation rate was 69.0% (87/126). The univariate analysis showed that BRAF mutation status was significantly associated with central lymph node metastasis (P<0.05), while the gender, multiple lesions, tumor size, extra-thyroidal invasion, Hashimoto's thyroiditis and tumor stage were not significantly associated with the BRAF mutation (P>0.05 for all). The multivariate analysis showed that only central lymph node metastasis was significantly correlated with BRAF mutation (P<0.05). When the diameter of tumor was ≤10 mm, BRAF mutation was statistically not significantly correlated to central lymph node metastasis (P>0.05). When the diameter of tumor was >10 mm, the central lymph node metastasis rate was significantly higher in patients with positive BRAF mutation than that in patients with a negative BRAF mutation (P<0.05).
  
  
  CONCLUSIONS
  The presence of BRAF mutation is an independent predictive factor for central lymph node metastasis. When PTC is with preoperative positive BRAF mutation, the cervical dissection should be routinely performed. The larger the tumor diameter is, the more important is the central lymph node dissection. There should be re-evaluated the necessity of preventative central lymph node dissection when the tumor diameter was ≤5 mm in patients with negative BRAF mutation.
authors.affiliation: The Fourth Department of General Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China.
authors.forename: Chenlei
authors.initials: C
authors.lastname: Shi
authors.affiliation: The Fourth Department of General Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China; Email: aitiantang83@126.com.
authors.forename: Huadong
authors.initials: H
authors.lastname: Qin
authors.affiliation: The Fourth Department of General Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Ding
authors.affiliation: The Fourth Department of General Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Sun
authors.affiliation: The Fourth Department of General Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China.
authors.forename: Yichen
authors.initials: Y
authors.lastname: Lyu
authors.affiliation: The Fourth Department of General Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China.
authors.forename: Tiefeng
authors.initials: T
authors.lastname: Shi
mesh_terms: D002277:Carcinoma / Q000453:epidemiology / Q000235:genetics* / Q000378:metabolism; D002291:Carcinoma, Papillary; D050031:Hashimoto Disease; D006801:Humans; D008197:Lymph Node Excision; D008198:Lymph Nodes; D008207:Lymphatic Metastasis / Q000175:diagnosis* / Q000235:genetics; D009154:Mutation; D016133:Polymerase Chain Reaction; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics*; D012189:Retrospective Studies; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000453:epidemiology / Q000235:genetics* / Q000378:metabolism
chemical_list: D048493:Proto-Oncogene Proteins B-raf