title: Bioinformatics Analysis of Potential Candidates for Therapy of TDRD7 Deficiency-Induced Congenital Cataract.
abstract: OBJECTIVE
  The aim of this study was to identify potential candidates and explore the possible mechanism in congenital cataract induced by tudor domain-containing 7 (TDRD7) deficiency.
  
  
  METHODS
  The gene expression profile GSE25812 generated from 18 samples was downloaded from the Gene Expression Omnibus database. Differentially expressed genes (DEGs) between disease and normal groups were identified. Then, gene ontology and pathway enrichment analysis of DEGs were performed. The protein-protein interaction (PPI) network and transcription factor (TF) regulatory network were constructed. The modules in the PPI network were identified. Significant target genes were selected from the TF regulatory network.
  
  
  RESULTS
  A total of 329 DEGs were obtained, and downregulated DEGs were significantly enriched in biological processes including defense response and immune response. In the PPI network, high-degree genes of complement component 1, q subcomponent, A/B/C chain (C1QA/C1QB/C1QC), lymphocyte antigen 86 (LY86) and neuroblastoma RAS viral oncogene homolog (NRAS) were identified. From the TF regulatory network, the heat shock 27 kDa protein 1 (HSPB1) was the target of the estrogen receptor 1, and LY86 was the target of the v-myc avian myelocytomatosis viral oncogene homolog.
  
  
  CONCLUSIONS
  HSPB1, NRAS, immune response, defense response and the related genes LY86, C1QA/C1QB/C1QC may play an important role in the development of congenital cataract induced by TDRD7 deficiency. However, further experiments are still needed.
authors.affiliation: Department of Ophthalmology, Air Force General Hospital of PLA, Beijing, China.
authors.forename: De-Wang
authors.initials: DW
authors.lastname: Shao
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: Yang
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.forename: Hai-Xia
authors.initials: HX
authors.lastname: Ru
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D002386:Cataract / Q000151:congenital* / Q000235:genetics* / Q000378:metabolism; D019295:Computational Biology; D005136:Eye Proteins / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D055785:Gene Knockdown Techniques; D053263:Gene Regulatory Networks; D051379:Mice; D046228:Microarray Analysis; D012261:Ribonucleoproteins / Q000172:deficiency* / Q000235:genetics; D015398:Signal Transduction / Q000502:physiology; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D005136:Eye Proteins; D012261:Ribonucleoproteins; C557201:Tdrd7 protein, human; D014157:Transcription Factors