title: Aerobic Exercise Recovers Disuse-induced Atrophy Through the Stimulus of the LRP130/PGC-1α Complex in Aged Rats.
abstract: Physical training has been shown to be important to the control of muscle mass during aging, through the activation of several pathways including, IGF1-AKT and PGC-1α. Also, it was demonstrated that LRP130, a component of the PGC-1α complex, is important for the PGC-1α-dependent transcription of several mitochondrial genes in vivo. To explore the role of physical training during aging, we investigated the effects on muscle recovery after short-term immobilization followed by 3 or 7 days with aerobic or resistance training. Using morphological (myofibrillar adenosine triphosphatase activity, to assess the total muscle fiber cross-sectional area (CSA) and the frequency of specific fiber types), biochemical (myosin heavy chain), and molecular analyses (quantitative real-time PCR, functional pathways analyses, and Western blot), our results indicated that after an atrophic stimulus, only animals subjected to aerobic training showed entire recovery of cross-sectional area; aerobic training reduced the ubiquitin-proteasome system components involved in muscle atrophy after 3 days of recovery, and the upregulation in PGC-1α expression enhanced the process of muscle recovery by inhibiting the FoxO pathway, with the possible involvement of LRP130. These results suggest that aerobic training enhanced the muscle regeneration process after disuse-induced atrophy in aged rats possibly through of the LRP130/PGC-1α complex by inhibiting the ubiquitin-proteasome system.
authors.affiliation: Department of Morphology, São Paulo State University, Institute of Biosciences, Botucatu, Brazil. ijvechetti@gmail.com.
authors.forename: Ivan J
authors.initials: IJ
authors.lastname: Vechetti-Junior
authors.affiliation: Department of Morphology, São Paulo State University, Institute of Biosciences, Botucatu, Brazil.
authors.forename: Raquel S
authors.initials: RS
authors.lastname: Bertaglia
authors.affiliation: Department of Morphology, São Paulo State University, Institute of Biosciences, Botucatu, Brazil.
authors.forename: Geysson J
authors.initials: GJ
authors.lastname: Fernandez
authors.affiliation: Department of Morphology, São Paulo State University, Institute of Biosciences, Botucatu, Brazil.
authors.forename: Tassiana G
authors.initials: TG
authors.lastname: de Paula
authors.affiliation: Department of Molecular Biology, University of São Paulo, Institute of Biosciences, Brazil.
authors.forename: Rodrigo W A
authors.initials: RW
authors.lastname: de Souza
authors.affiliation: Department of Morphology, São Paulo State University, Institute of Biosciences, Botucatu, Brazil.
authors.forename: Leonardo N
authors.initials: LN
authors.lastname: Moraes
authors.affiliation: Department of Morphology, São Paulo State University, Institute of Biosciences, Botucatu, Brazil.
authors.forename: Edson A
authors.initials: EA
authors.lastname: Mareco
authors.affiliation: Department of Morphology, São Paulo State University, Institute of Biosciences, Botucatu, Brazil. Department of Physiotherapy, University of Oeste Paulista, Presidente Prudente, São Paulo, Brazil.
authors.forename: Carlos E A
authors.initials: CE
authors.lastname: de Freitas
authors.affiliation: Center of Biological and Health Sciences, North University of Paraná, Londrina, Brazil.
authors.forename: Andreo F
authors.initials: AF
authors.lastname: Aguiar
authors.affiliation: Department of Morphology, São Paulo State University, Institute of Biosciences, Botucatu, Brazil.
authors.forename: Robson F
authors.initials: RF
authors.lastname: Carvalho
authors.affiliation: Department of Morphology, São Paulo State University, Institute of Biosciences, Botucatu, Brazil.
authors.forename: Maeli
authors.initials: M
authors.lastname: Dal-Pai-Silva
mesh_terms: D000367:Age Factors; D000818:Animals; D051858:Forkhead Transcription Factors / Q000502:physiology; D007103:Immobilization; D008297:Male; D009124:Muscle Proteins / Q000502:physiology; D018482:Muscle, Skeletal / Q000503:physiopathology; D009133:Muscular Atrophy / Q000209:etiology / Q000628:therapy*; D009419:Nerve Tissue Proteins / Q000502:physiology; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function / Q000502:physiology*; D055070:Resistance Training; D044843:SKP Cullin F-Box Protein Ligases / Q000502:physiology; D014157:Transcription Factors / Q000502:physiology*; D000070584:Tripartite Motif Proteins; D044767:Ubiquitin-Protein Ligases / Q000502:physiology; D000071161:Forkhead Box Protein O1
chemical_list: D051858:Forkhead Transcription Factors; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D044843:SKP Cullin F-Box Protein Ligases; D014157:Transcription Factors; D000070584:Tripartite Motif Proteins; D044767:Ubiquitin-Protein Ligases; C501429:Ppargc1a protein, rat; C074639:Foxo1 protein, rat; D000071161:Forkhead Box Protein O1; C486692:Fbxo32 protein, rat; C499086:Trim63 protein, rat
keywords: Aging; Exercise training; Immobilization; Muscle mass; Recovery