title: Optical electrocorticogram (OECoG) using wide-field calcium imaging reveals the divergence of neuronal and glial activity during acute rodent seizures.
abstract: The role of glia in epilepsy has been widely debated. Using in vivo bulk loading of calcium dyes, we imaged neuronal and glial activity in an acute pharmacologic rodent model of neocortical seizures. Optical calcium-based ECoG maps revealed that neuronal waves propagated rapidly and remained mostly confined to the seizure focus. Glial waves were triggered by ictal onset but propagated slowly in a stereotypical fashion far beyond the seizure focus. Although related at their onset, the divergence of these two phenomena during seizure evolution calls into question their interdependence and the criticality of the role of glia in seizure onset and neurovascular coupling. This article is part of a Special Issue entitled "Status Epilepticus".
authors.affiliation: Weill Cornell Medical College, New York Presbyterian Hospital, Department of Neurological Surgery, Brain and Mind Research Institute, Brain and Spine Center, 1300 York Avenue, Box 99, New York, NY 10021, USA.
authors.forename: Andy G S
authors.initials: AG
authors.lastname: Daniel
authors.affiliation: Weill Cornell Medical College, New York Presbyterian Hospital, Department of Neurological Surgery, Brain and Mind Research Institute, Brain and Spine Center, 1300 York Avenue, Box 99, New York, NY 10021, USA; The Browning School, 52 E 62nd St, New York, NY 10065, USA.
authors.forename: Philippe
authors.initials: P
authors.lastname: Laffont
authors.affiliation: Weill Cornell Medical College, New York Presbyterian Hospital, Department of Neurological Surgery, Brain and Mind Research Institute, Brain and Spine Center, 1300 York Avenue, Box 99, New York, NY 10021, USA.
authors.forename: Mingrui
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Weill Cornell Medical College, New York Presbyterian Hospital, Department of Neurological Surgery, Brain and Mind Research Institute, Brain and Spine Center, 1300 York Avenue, Box 99, New York, NY 10021, USA. Electronic address: hom2001@med.cornell.edu.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Ma
authors.affiliation: Weill Cornell Medical College, New York Presbyterian Hospital, Department of Neurological Surgery, Brain and Mind Research Institute, Brain and Spine Center, 1300 York Avenue, Box 99, New York, NY 10021, USA.
authors.forename: Theodore H
authors.initials: TH
authors.lastname: Schwartz
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000814:Aniline Compounds / Q000032:analysis / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000032:analysis / Q000378:metabolism*; D003952:Diagnostic Imaging / Q000379:methods; D000069280:Electrocorticography / Q000379:methods*; D005452:Fluoresceins / Q000032:analysis / Q000378:metabolism; D008297:Male; D009457:Neuroglia / Q000737:chemistry / Q000378:metabolism*; D009474:Neurons / Q000737:chemistry / Q000378:metabolism*; D055096:Optical Devices; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000378:metabolism* / Q000473:pathology*
chemical_list: D000814:Aniline Compounds; D005452:Fluoresceins; C503942:Oregon green 488 BAPTA-1; D002118:Calcium
keywords: 4-Aminopyridine; Astrocyte; Calcium; Epilepsy; Glia; Ictal; Imaging; Interictal; Rat; Seizure