title: Simple and rapid preparation of red fluorescence and red color S. aureus derived nanobioparticles for pathogen detection.
abstract: In this study, a simple and rapid method was developed to transform protein A producing Staphylococcus aureus cells into red color and red fluorescent nanobioparticles, which were homogeneous, dispersive and relatively stable with a uniform size of 800 nm. The method consists of reaction with a monotetrazolium redox dye at 25°C for 15 min and heat inactivation at 65°C for 30 min. This method provided the first S. aureus nanobioparticles with the dual property of red color and red fluorescence. Attributed to the IgG binding site known as protein A on their surface, the nanobioparticles could be used as vectors for immunoassays of many bacteria and viruses. Coagglutination test of Escherichia coli O157:H7 observed by naked eyes showed that the detection limitation of the nanobioprobes was 1∗10(6) CFU/ml, which was about 100 times more sensitive than the natural uncolored S. aureus bioprobes. Red fluorescence detection and analysis of the coagglutination product by a microplate reader lowered the detection limit to 2.5∗10(4) CFU/ml.
authors.affiliation: Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China; University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Hu
authors.affiliation: Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China; University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Hang
authors.initials: H
authors.lastname: Yang
authors.affiliation: Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Junping
authors.initials: J
authors.lastname: Yu
authors.affiliation: Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. Electronic address: hpwei@wh.iov.cn.
authors.forename: Hongping
authors.initials: H
authors.lastname: Wei
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D005453:Fluorescence; D053758:Nanoparticles / Q000737:chemistry; D013205:Staphylococcal Protein A / Q000737:chemistry / Q000378:metabolism; D013211:Staphylococcus aureus / Q000737:chemistry* / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins; D013205:Staphylococcal Protein A
keywords: 5-Cyano-2, 3-ditolyl tetrazolium chloride (CTC); Coagglutination test; E. coli O157:H7; Fluorescence detection; Nanobioprobe; Nanomaterial biosynthesis; Red and fluorescent S. aureus