title: Intra-arterial Administration of Placenta-Derived Decidual Stromal Cells to the Superior Mesenteric Artery in the Rabbit: Distribution of Cells, Feasibility, and Safety.
abstract: Selective administration of mesenchymal stromal cells to the mesenteric arteries is a potential technique to overcome pulmonary trapping and increase the density of transplanted cells in extensive mural inflammation of the intestine, such as in inflammatory bowel disease and graft-versus-host disease. We injected 5 × 10(6) (111)In-oxine-labeled human decidual stromal cells (DSCs) to the rabbit superior mesenteric artery (SMA) using clinical routine catheters guided by an angiographical system under sterile conditions. We used longitudinal single-photon emission tomography at 6 h and at 1, 2, and 5 days to assess trafficking and distribution of DSCs. We used digital subtraction angiography, computed tomography, and hematoxylin and eosin stainings to determine biodistribution of cells and to assess safety end points. We found that selective injection of human DSCs to the rabbit SMA does not result in acute embolic complications. Furthermore, we found that IV administration resulted in extensive retention of the radiolabeled DSCs in the lungs, corroborating previous studies on pulmonary trapping. In sharp contrast, selective injections to the SMA resulted in uptake distributed in the intestine supplied by the SMA and in the liver, indicating that this approach could significantly increase the fraction of injected DSCs reaching the target tissue.
authors.affiliation: Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Fabian
authors.initials: F
authors.lastname: Arnberg
authors.forename: Johan
authors.initials: J
authors.lastname: Lundberg
authors.forename: Annie
authors.initials: A
authors.lastname: Olsson
authors.forename: Erik
authors.initials: E
authors.lastname: Samén
authors.forename: Nasren
authors.initials: N
authors.lastname: Jaff
authors.forename: Emma
authors.initials: E
authors.lastname: Jussing
authors.forename: Ulrika
authors.initials: U
authors.lastname: Dahlén
authors.forename: Silvia
authors.initials: S
authors.lastname: Nava
authors.forename: Rimma
authors.initials: R
authors.lastname: Axelsson
authors.forename: Olle
authors.initials: O
authors.lastname: Ringdén
authors.forename: Helen
authors.initials: H
authors.lastname: Kaipe
authors.forename: Staffan
authors.initials: S
authors.lastname: Holmin
mesh_terms: D015901:Angiography, Digital Subtraction / Q000379:methods; D000818:Animals; D005260:Female; D006086:Graft vs Host Disease; D006801:Humans; D007261:Infusions, Intra-Arterial / Q000379:methods; D008099:Liver / Q000166:cytology*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D017538:Mesenteric Artery, Superior / Q000637:transplantation*; D010920:Placenta / Q000166:cytology; D011247:Pregnancy; D011817:Rabbits; D017154:Stromal Cells / Q000166:cytology*; D014018:Tissue Distribution / Q000502:physiology*