title: Fast, Efficient, and Stable Conjugation of Multiple DNA Strands on Colloidal Quantum Dots.
abstract: A novel method for covalent conjugation of DNA to polymer coated quantum dots (QDs) is investigated in detail. This method is fast and efficient: up to 12 DNA strands can be covalently conjugated per QD in optimized reaction conditions. The QD-DNA conjugates can be purified using size exclusion chromatography and the QDs retain high quantum yield and excellent stability after DNA coupling. We explored single-stranded and double-stranded DNA coupling, as well as various lengths. We show that the DNA coupling is most efficient for short (15 mer) single-stranded DNA. The DNA coupling has been performed on QDs emitting at four different wavelengths, as well as on gold nanoparticles, suggesting that this technique can be generalized to a wide range of nanoparticles.
authors.affiliation: †Laboratoire de Physique et d'Etude des Matériaux, ESPCI ParisTech, CNRS UMR 8213, Université Pierre et Marie Curie, 10 Rue Vauquelin, 75005 Paris, France.
authors.forename: Anusuya
authors.initials: A
authors.lastname: Banerjee
authors.affiliation: ‡Nexdot, 10 rue Vauquelin, 75005 Paris, France.
authors.forename: Chloé
authors.initials: C
authors.lastname: Grazon
authors.affiliation: ‡Nexdot, 10 rue Vauquelin, 75005 Paris, France.
authors.forename: Brice
authors.initials: B
authors.lastname: Nadal
authors.affiliation: †Laboratoire de Physique et d'Etude des Matériaux, ESPCI ParisTech, CNRS UMR 8213, Université Pierre et Marie Curie, 10 Rue Vauquelin, 75005 Paris, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Pons
authors.affiliation: §E305A, Physical Sciences Division, Gordon Centre for Integrative Science, 929 57th Street, Chicago, Illinois 60637 United States.
authors.forename: Yamuna
authors.initials: Y
authors.lastname: Krishnan
authors.affiliation: †Laboratoire de Physique et d'Etude des Matériaux, ESPCI ParisTech, CNRS UMR 8213, Université Pierre et Marie Curie, 10 Rue Vauquelin, 75005 Paris, France.
authors.forename: Benoit
authors.initials: B
authors.lastname: Dubertret
mesh_terms: D003102:Colloids / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D045663:Quantum Dots
chemical_list: D003102:Colloids; D006046:Gold; D004247:DNA