title: Detection and quantification of bovine papillomavirus type 2 in urinary bladders and lymph nodes in cases of Bovine Enzootic Hematuria from the endemic region of Azores.
abstract: Bovine Enzootic Hematuria (BEH) is a disease with a severe impact on production indexes and characterized by the development of bovine urinary bladder tumors, particularly in the Azores archipelago. The purpose of this study was to investigate and quantify BPV2 tissue distribution in bovine urinary bladder tumors, normal bladders, and iliac lymph nodes of cattle from the Azores. A real-time PCR system targeting the L1 gene was developed and allowed for the specific detection of the virus. BPV2 DNA was detected in a large proportion of the samples tested, both from neoplastic and healthy tissues, indicating that this virus is very prevalent in the bovine population of the Azores. Moreover, all types of tissues tested were positive, confirming a wide viral distribution within the infected animal. Bovine cutaneous papillomas sampled from Portuguese mainland dairy cattle were used as controls. Viral load ranged between 2.2×10(4) copies/cell in the skin papillomas, and 0.0002 copies/cell in the urinary bladders tumors from the Azores. This is the first report presenting quantitative data on BPV2 infection in bovine urinary bladder lesions from the Azores. This approach will provide a useful tool to evaluate the role of BPV2 not only in the pathogenesis BEH but also in cell transformation mechanisms.
authors.affiliation: Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA), Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, 1300-477 Lisboa, Portugal. Electronic address: adjoaobcota@fmv.ulisboa.pt.
authors.forename: João B
authors.initials: JB
authors.lastname: Cota
authors.affiliation: Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA), Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, 1300-477 Lisboa, Portugal.
authors.forename: Maria C
authors.initials: MC
authors.lastname: Peleteiro
authors.affiliation: Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA.
authors.forename: Lisa
authors.initials: L
authors.lastname: Petti
authors.affiliation: Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA), Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, 1300-477 Lisboa, Portugal.
authors.forename: Luís
authors.initials: L
authors.lastname: Tavares
authors.affiliation: Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA), Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, 1300-477 Lisboa, Portugal.
authors.forename: Ana
authors.initials: A
authors.lastname: Duarte
mesh_terms: D000818:Animals; D001394:Azores; D052688:Bovine papillomavirus 1 / Q000235:genetics / Q000378:metabolism*; D036022:Capsid Proteins / Q000235:genetics; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D006417:Hematuria / Q000662:veterinary* / Q000821:virology; D008198:Lymph Nodes / Q000821:virology*; D010212:Papilloma / Q000821:virology; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D001749:Urinary Bladder Neoplasms / Q000662:veterinary* / Q000821:virology; D019562:Viral Load
chemical_list: D036022:Capsid Proteins; C085217:L1 protein, Bovine papillomavirus
keywords: BPV2; Cell transformation; Quantitative polymerase chain reaction; Viral load