title: Isolation and identification of minor secoiridoids and phenolic components from thermally treated olive oil by-products.
abstract: The application of an industrial process based on the hydrothermal treatment of 160 °C/60 min of alperujo, a by-product of olive oil extraction, allows the formation of a liquid phase containing a high concentration of phenolic and secoiridoid compounds. Ethyl acetate was used to extract these phenolic compounds from the aqueous matrix. In this study, the isolation with polyamide and XAD resin allowed detection of the presence of phenolic compounds in minor concentrations. These minor phenols were several oleuropein derivatives that had not been identified in these phenolic extracts previously. The polar compounds, acteosides, secoiridoids, and flavonoids, that remain in the aqueous fraction after extraction with ethyl acetate were identified. We report the presence of known compounds and also detected a novel molecule in alperujo with a molecular weight of 408 whose structure was characterized for first time. This new secoiridoid glucoside was identified as 1-β-D-glucopyranosyl acyclodihydroelenolic acid.
authors.affiliation: Instituto de la Grasa, Consejo Superior de Investigaciones Científicas (CSIC), Ctra Utrera km 1, Campus Universitario Pablo de Olavide, Edificio 46, Seville 41013, Spain.
authors.forename: Fátima
authors.initials: F
authors.lastname: Rubio-Senent
authors.affiliation: Universidad de Sevilla, Departamento de Química Orgánica, Facultad de Química, Profesor García González 1, Seville 41012, Spain.
authors.forename: Sergio
authors.initials: S
authors.lastname: Martos
authors.affiliation: Instituto de la Grasa, Consejo Superior de Investigaciones Científicas (CSIC), Ctra Utrera km 1, Campus Universitario Pablo de Olavide, Edificio 46, Seville 41013, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Lama-Muñoz
authors.affiliation: Universidad de Sevilla, Departamento de Química Orgánica, Facultad de Química, Profesor García González 1, Seville 41012, Spain.
authors.forename: José G
authors.initials: JG
authors.lastname: Fernández-Bolaños
authors.affiliation: Instituto de la Grasa, Consejo Superior de Investigaciones Científicas (CSIC), Ctra Utrera km 1, Campus Universitario Pablo de Olavide, Edificio 46, Seville 41013, Spain.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Rodríguez-Gutiérrez
authors.affiliation: Instituto de la Grasa, Consejo Superior de Investigaciones Científicas (CSIC), Ctra Utrera km 1, Campus Universitario Pablo de Olavide, Edificio 46, Seville 41013, Spain. Electronic address: jfbg@cica.es.
authors.forename: Juan
authors.initials: J
authors.lastname: Fernández-Bolaños
mesh_terms: D005419:Flavonoids / Q000737:chemistry; D006358:Hot Temperature; D039823:Iridoids / Q000737:chemistry*; D015394:Molecular Structure; D000069463:Olive Oil / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry*; D014866:Waste Products / Q000032:analysis*
chemical_list: D005419:Flavonoids; D039823:Iridoids; D000069463:Olive Oil; D010636:Phenols; D014866:Waste Products
keywords: Acteosides; Ethyl acetate extract; Flavonoids; Phenolic compounds; Pomace olive waste (alperujo); Secoiridoid derivatives; Steam-treatment