title: Human Platelet Polymorphism can be a genetic marker associated with HIV/HCV coinfection.
abstract: To evaluate the associations of HPA polymorphisms -1, -3, and -5 with HIV/HCV coinfection were included in this study 60 HIV/HCV-coinfected patients from the Sao Paulo State health service centers. Data reported by Verdichio-Moraes et al. (2009: J. Med Virol 81:757-759) were used as the non-infected and HCV monoinfected groups. Human Platelet Polymorphism genotyping was performed in 60 Patients co-infected with HIV/HCV by PCR-SSP or PCR-RFLP. HIV subtyping and HCV genotyping was performed by RT-PCR followed sequencing. The data analyses were performed using the χ2 test or Fisher's Exact Test and the logistic regression model. Patients coinfected with HIV/HCV presented HCV either genotype 1 (78.3%) or non-1 (21.7%) and HIV either subtype B (85.0%) or non-B (15%). The Human Platelet Polymorphism-1a/1b genotype was more frequent (P < 0.05) in HIV/HCV coinfection than in HCV monoinfection and the allelic frequency of Human Platelet Polymorphism-5b in the Patients coinfected with HIV/HCV was higher (P < 0.05) than in HCV monoinfected cases and non-infected individuals. These data suggest that the presence of specific HPA allele on platelets could favor the existence of coinfection. On the other hand, Human Platelet Polymorphism-5a/5b was more frequent (P < 0.05) in HIV/HCV coinfected and HCV monoinfected groups than in the non-infected individuals, suggesting that this platelet genotype is related to HCV infection, regardless of HIV presence. Results suggest that the Human Platelet Polymorphism profile in HIV/HCV coinfected individuals differs from the one of both HCV monoinfected and non-infected population. So, the Human Platelet Polymorphism can be a genetic marker associated with HIV/HCV coinfection.
authors.affiliation: Molecular Biology Laboratory, Blood Transfusion Center, Botucatu Medical School, Sao Paulo State University, UNESP, São Paulo, Brazil.|Department of Bioprocess and Biotechnology, School of Agricultural Sciences, Lageado Experiment Station. Sao Paulo State University, UNESP, Botucatu-SP, Brazil.
authors.forename: Rejane Maria Tommasini
authors.initials: RM
authors.lastname: Grotto
authors.affiliation: Molecular Biology Laboratory, Blood Transfusion Center, Botucatu Medical School, Sao Paulo State University, UNESP, São Paulo, Brazil.
authors.forename: Natália
authors.initials: N
authors.lastname: Picelli
authors.affiliation: Tropical Diseases Department, Botucatu Medical School, Sao Paulo State University UNESP, São Paulo, Brazil.
authors.forename: Lenice do Rosário
authors.initials: Ldo R
authors.lastname: de Souza
authors.affiliation: Department of Internal Medicine, Botucatu Medical School, Sao Paulo State University UNESP, São Paulo, Brazil.
authors.forename: Giovanni Faria
authors.initials: GF
authors.lastname: Silva
authors.affiliation: Molecular Biology Laboratory, Blood Transfusion Center, Botucatu Medical School, Sao Paulo State University, UNESP, São Paulo, Brazil.|Department of Internal Medicine, Botucatu Medical School, Sao Paulo State University UNESP, São Paulo, Brazil.
authors.forename: Adriana Camargo
authors.initials: AC
authors.lastname: Ferrasi
authors.affiliation: Department of Biostatistics, Institute of Bioscience, UNESP, São Paulo, Brazil.
authors.forename: Liciana Vaz de Arruda
authors.initials: LV
authors.lastname: Silveira
authors.affiliation: Molecular Biology Laboratory, Blood Transfusion Center, Botucatu Medical School, Sao Paulo State University, UNESP, São Paulo, Brazil.|Department of Internal Medicine, Botucatu Medical School, Sao Paulo State University UNESP, São Paulo, Brazil.
authors.forename: Maria Inês de Moura Campos
authors.initials: MI
authors.lastname: Pardini
mesh_terms: D000328:Adult; D016824:Antigens, Human Platelet / Q000235:genetics*; D060085:Coinfection / Q000235:genetics; D005260:Female; D005819:Genetic Markers; D005838:Genotype; D015658:HIV Infections / Q000150:complications* / Q000235:genetics; D006526:Hepatitis C / Q000150:complications* / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012367:RNA, Viral / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D016824:Antigens, Human Platelet; D005819:Genetic Markers; D012367:RNA, Viral
keywords: HIV/HCV coinfection; human platelet polymorphism -1; human platelet polymorphism -3; human platelet polymorphism -5; polymorphism