title: Genome evolution of intermediate wheatgrass as revealed by EST-SSR markers developed from its three progenitor diploid species.
abstract: Intermediate wheatgrass (Thinopyrum intermedium (Host) Barkworth & D.R. Dewey), a segmental autoallohexaploid (2n = 6x = 42), is not only an important forage crop but also a valuable gene reservoir for wheat (Triticum aestivum L.) improvement. Throughout the scientific literature, there continues to be disagreement as to the origin of the different genomes in intermediate wheatgrass. Genotypic data obtained from newly developed EST-SSR primers derived from the putative progenitor diploid species Pseudoroegneria spicata (Pursh) Á. Löve (St genome), Thinopyrum bessarabicum (Savul. & Rayss) Á. Löve (J = J(b) = E(b)), and Thinopyrum elongatum (Host) D. Dewey (E = J(e) = E(e)) indicate that the V genome of Dasypyrum (Coss. & Durieu) T. Durand is not one of the three genomes in intermediate wheatgrass. Based on all available information in the literature and findings in this study, the genomic designation of intermediate wheatgrass should be changed to J(vs)J(r)St, where J(vs) and J(r) represent ancestral genomes of present-day J(b) of Th. bessarabicum and J(e) of Th. elongatum, with J(vs) being more ancient. Furthermore, the information suggests that the St genome in intermediate wheatgrass is most similar to the present-day St found in diploid species of Pseudoroegneria from Eurasia.
authors.affiliation: a US Department of Agriculture, Agricultural Research Services, Forage and Range Research Laboratory, Logan, UT 84322-6300, USA.
authors.forename: Richard R-C
authors.initials: RR
authors.lastname: Wang
authors.affiliation: a US Department of Agriculture, Agricultural Research Services, Forage and Range Research Laboratory, Logan, UT 84322-6300, USA.
authors.forename: Steve R
authors.initials: SR
authors.lastname: Larson
authors.affiliation: a US Department of Agriculture, Agricultural Research Services, Forage and Range Research Laboratory, Logan, UT 84322-6300, USA.
authors.forename: Kevin B
authors.initials: KB
authors.lastname: Jensen
authors.affiliation: a US Department of Agriculture, Agricultural Research Services, Forage and Range Research Laboratory, Logan, UT 84322-6300, USA.
authors.forename: B Shaun
authors.initials: BS
authors.lastname: Bushman
authors.affiliation: b The Land Institute, 2440 E. Water Well Road, Salina, KS 67401, USA.
authors.forename: Lee R
authors.initials: LR
authors.lastname: DeHaan
authors.affiliation: b The Land Institute, 2440 E. Water Well Road, Salina, KS 67401, USA.
authors.forename: Shuwen
authors.initials: S
authors.lastname: Wang
authors.affiliation: a US Department of Agriculture, Agricultural Research Services, Forage and Range Research Laboratory, Logan, UT 84322-6300, USA.|c College of Animal Husbandry and Veterinary Science, Henan Agricultural University, Zhengzhou, 450002, China.
authors.forename: Xuebing
authors.initials: X
authors.lastname: Yan
mesh_terms: D016000:Cluster Analysis; D018744:DNA, Plant / Q000235:genetics; D004171:Diploidy; D019143:Evolution, Molecular; D020224:Expressed Sequence Tags; D005819:Genetic Markers; D018745:Genome, Plant; D005838:Genotype; D018895:Microsatellite Repeats; D006109:Poaceae / Q000145:classification / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers
keywords: Dasypyrum; Pseudoroegneria; Thinopyrum; formule génomique; genome constitution; marqueur moléculaire; molecular marker; phylogeny; phylogénie