title: Accumulation of indium-111-labeled human low density lipoprotein in the rabbit aorta: Implications for nuclear imaging of vascular lesions.
abstract: Nuclear imaging of atheromata must distinguish lesions from both blood pool and normal arterial tissue. We have examined spatial and temporal variations of indium-111-labeled human low density lipoprotein (LDL) accumulation in rabbit aortas. LDL-derived In-111 activity was time-independent in lesion-resistant regions of aortas from normal and hypercholesterolemic animals (mean 2.9 × 10(-6) percent injected activity per milligram tissue [%IA/mg]) and in lesion-prone regions of normal aortas (mean 7.1 × 10(-6) %IA/mg). In contrast, activity in sudanophilic lesions of hypercholesterolemic rabbit aortas reached a peak of 31 × 10(-6) %IA/mg at 92 hours postinjection. The mean ratio between activity in lesions versus lesion-resistant regions described a broad convex curve with minima of 4:1 at 14 hours and 136 hours and a peak of 14:1 measured at 72 hours postinjection. The mean ratio between In-111 in lesions and blood followed a sigmoid curve, rising exponentially from 1:25 at 14 hours to 1:3 by 72 hours postinjection. We conclude that optimal signal-to-noise ratios for monitoring atheroma-associated LDL-derived radioactivity occur late, not before about 3 days postinjection. Therefore, LDL labeled with In-111 or even longer-lived radionuclides holds the greatest promise for effective clinical nuclear imaging of atherosclerosis.
authors.affiliation: Departments of Pathology, Medicine, Radiology, Surgery, and Radiation and Cellular Oncology, The University of Chicago, Chicago, Illinois, USA; Chemistry Division, Argonne National Laboratory, Argonne, Illinois, USA; Radiation Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: R V
authors.initials: RV
authors.lastname: Hay
authors.affiliation: Departments of Pathology, Medicine, Radiology, Surgery, and Radiation and Cellular Oncology, The University of Chicago, Chicago, Illinois, USA; Chemistry Division, Argonne National Laboratory, Argonne, Illinois, USA; Radiation Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: D D
authors.initials: DD
authors.lastname: Casalino
authors.affiliation: Departments of Pathology, Medicine, Radiology, Surgery, and Radiation and Cellular Oncology, The University of Chicago, Chicago, Illinois, USA; Chemistry Division, Argonne National Laboratory, Argonne, Illinois, USA; Radiation Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: L
authors.initials: L
authors.lastname: Kordylewski
authors.affiliation: Departments of Pathology, Medicine, Radiology, Surgery, and Radiation and Cellular Oncology, The University of Chicago, Chicago, Illinois, USA; Chemistry Division, Argonne National Laboratory, Argonne, Illinois, USA; Radiation Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: R W
authors.initials: RW
authors.lastname: Atcher
authors.affiliation: Departments of Pathology, Medicine, Radiology, Surgery, and Radiation and Cellular Oncology, The University of Chicago, Chicago, Illinois, USA; Chemistry Division, Argonne National Laboratory, Argonne, Illinois, USA; Radiation Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: M W
authors.initials: MW
authors.lastname: Brechbiel
authors.affiliation: Departments of Pathology, Medicine, Radiology, Surgery, and Radiation and Cellular Oncology, The University of Chicago, Chicago, Illinois, USA; Chemistry Division, Argonne National Laboratory, Argonne, Illinois, USA; Radiation Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: O A
authors.initials: OA
authors.lastname: Gansow
authors.affiliation: Departments of Pathology, Medicine, Radiology, Surgery, and Radiation and Cellular Oncology, The University of Chicago, Chicago, Illinois, USA; Chemistry Division, Argonne National Laboratory, Argonne, Illinois, USA; Radiation Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: U
authors.initials: U
authors.lastname: Sharokhizadeh
authors.affiliation: Departments of Pathology, Medicine, Radiology, Surgery, and Radiation and Cellular Oncology, The University of Chicago, Chicago, Illinois, USA; Chemistry Division, Argonne National Laboratory, Argonne, Illinois, USA; Radiation Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: R M
authors.initials: RM
authors.lastname: Fleming
authors.affiliation: Departments of Pathology, Medicine, Radiology, Surgery, and Radiation and Cellular Oncology, The University of Chicago, Chicago, Illinois, USA; Chemistry Division, Argonne National Laboratory, Argonne, Illinois, USA; Radiation Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: K A
authors.initials: KA
authors.lastname: Lathrop
authors.affiliation: Departments of Pathology, Medicine, Radiology, Surgery, and Radiation and Cellular Oncology, The University of Chicago, Chicago, Illinois, USA; Chemistry Division, Argonne National Laboratory, Argonne, Illinois, USA; Radiation Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: V J
authors.initials: VJ
authors.lastname: Stark
authors.affiliation: Departments of Pathology, Medicine, Radiology, Surgery, and Radiation and Cellular Oncology, The University of Chicago, Chicago, Illinois, USA; Chemistry Division, Argonne National Laboratory, Argonne, Illinois, USA; Radiation Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: P V
authors.initials: PV
authors.lastname: Harper