title: New ambuic acid derivatives from the solid culture of Pestalotiopsis neglecta and their nitric oxide inhibitory activity.
abstract: Four new ambuic acid derivatives (1-4), and four known derivatives (5-8), were isolated from the solid culture of a plant pathogenic fungus Pestalotiopsis neglecta. Their structures were elucidated by extensive NMR experiments. The absolute configuration of the C-16 secondary alcohol in 1 was deduced via the CD data of the in situ formed [Rh2(OCOCF3)4] complex with the acetonide derivative of 1. The absolute configuration in 3 was assigned by comparison of the experimental and simulated electronic circular dichroism (ECD) spectrum. The NMR data of compound 5 was reported for the first time. In the nitric oxide (NO) inhibition assay, compounds 4, 6 and 7 showed inhibitory activity against the NO production in the lipopolysaccharide (LPS)-induced macrophage with IC50 values of 88.66, 11.20, and 20.80 µM, respectively.
authors.affiliation: 1] State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, NO. 1 Beichen West Road, Chaoyang District, Beijing 100101, People's Republic of China [2] University of Chinese Academy of Sciences, No.19A Yuquan Road, Beijing 100049, People's Republic of China.
authors.forename: Qiu-Yue
authors.initials: QY
authors.lastname: Qi
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, NO. 1 Beichen West Road, Chaoyang District, Beijing 100101, People's Republic of China.
authors.forename: Er-Wei
authors.initials: EW
authors.lastname: Li
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, NO. 1 Beichen West Road, Chaoyang District, Beijing 100101, People's Republic of China.
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Han
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, NO. 1 Beichen West Road, Chaoyang District, Beijing 100101, People's Republic of China.
authors.forename: Yun-Fei
authors.initials: YF
authors.lastname: Pei
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, NO. 1 Beichen West Road, Chaoyang District, Beijing 100101, People's Republic of China.
authors.forename: Ke
authors.initials: K
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, NO. 1 Beichen West Road, Chaoyang District, Beijing 100101, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Bao
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, NO. 1 Beichen West Road, Chaoyang District, Beijing 100101, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.affiliation: School of Pharmacy, Yantai University, No. 32 Qingquan Road, Laishan District, Yantai, 264005, People's Republic of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, NO. 1 Beichen West Road, Chaoyang District, Beijing 100101, People's Republic of China.
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Liu
mesh_terms: D001203:Ascomycota / Q000378:metabolism*; D003512:Cyclohexanones / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy; D009569:Nitric Oxide / Q000037:antagonists & inhibitors*
chemical_list: D003512:Cyclohexanones; C429363:ambuic acid; D009569:Nitric Oxide