title: The first identification of a blood-sucking abomasal nematode Ashworthius sidemi in cattle (Bos taurus) using simple polymerase chain reaction (PCR).
abstract: A simple polymerase chain reaction (PCR) test was used to identify Ashworthius sidemi, a blood-sucking gastrointestinal nematode that commonly infects bison, red and roe deer, and moose in Poland. The present study uses this technique to confirm the possibility of transmission of A. sidemi infection from wildlife to domestic animals, such as cattle and sheep, grazing on the same natural pastures. A 406 bp fragment of genomic A. sidemi DNA was actually detected in DNA isolated from larval cultures derived from feces from cattle. A. sidemi DNA has been detected in cattle which represent a new host for this parasite. This is the first evidence of A. sidemi in cattle. The results reveal that a PCR test based on DNA from L3 larvae can be used for in vivo detection of A. sidemi invasions in breeding animals. In conclusion, the transfer of A. sidemi infection from wildlife to the farm animals sharing the same pastures appears possible.
authors.affiliation: Witold Stefański Institute of Parasitology of the Polish Academy of Sciences, Twarda Street 51/55, 00-818 Warsaw, Poland. Electronic address: moskwa@twarda.pan.pl.
authors.forename: Bożena
authors.initials: B
authors.lastname: Moskwa
authors.affiliation: Witold Stefański Institute of Parasitology of the Polish Academy of Sciences, Twarda Street 51/55, 00-818 Warsaw, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Bień
authors.affiliation: Witold Stefański Institute of Parasitology of the Polish Academy of Sciences, Twarda Street 51/55, 00-818 Warsaw, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Cybulska
authors.affiliation: Witold Stefański Institute of Parasitology of the Polish Academy of Sciences, Twarda Street 51/55, 00-818 Warsaw, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Kornacka
authors.affiliation: Białowieża National Park, Park Pałacowy 11, 17-230 Białowieża, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Krzysiak
authors.affiliation: National Veterinary Research Institute, Aleja Partyzantów 57, 24-100 Puławy, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Cencek
authors.affiliation: Witold Stefański Institute of Parasitology of the Polish Academy of Sciences, Twarda Street 51/55, 00-818 Warsaw, Poland.
authors.forename: Władysław
authors.initials: W
authors.lastname: Cabaj
mesh_terms: D000018:Abomasum / Q000469:parasitology; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D005243:Feces / Q000469:parasitology; D007814:Larva; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D017422:Sequence Analysis, DNA / Q000662:veterinary; D014251:Trichostrongyloidea / Q000235:genetics / Q000302:isolation & purification*; D014252:Trichostrongyloidiasis / Q000469:parasitology / Q000662:veterinary*
keywords: Ashworthius sidemi; Bos taurus; Cattle; L3 DNA; PCR