title: Expression of relative-protein of hypoxia-inducible factor-1α in vasculogenesis of mouse embryo.
abstract: BACKGROUND
  Physiological vasculogenesis in embryonic tissues share some important features with pathological neoangiogenesis in tumors. Linearly Patterned Programmed Cell Necrosis (LPPCN) and Vasculogenic Mimicry (VM) have been reported in tumors. The term VM refers to the aggressive tumor cells with CD31-negative phenotype to form Periodic Αcid Schiff (PAS)-positive network, that mimics the pattern of embryonic vasculogenic networks. LPPCN had been observed in our laboratory, and served as a spatial infrastructure for VM and endothelium-dependent vessel formation. Studies have been shown that hypoxia-inducible factor-1α (HIF-1α) can induce tumor cells to form vessel-like tubes and express genes associated with VM. Therefore, an analogous investigation has been carried out to determine if these patterns existed in mouse embryonic vasculogenesis.
  
  
  RESULTS
  In this essay, the results demonstrated that the number of Linearly Patterned Cell Αpoptosis (LPCA), embryo Vasculogenic Μimicry (embryo VM), endothelium-dependent vessels, and relative-protein of HIF-1α expression all showed time-dependent tendencies on E5.5-E9.5 (p < 0.05). The proteins CD133, VEGF, Twist, E-cadherin, and Vimentin showed local plexus distribution on E6.5-E7.5 (p < 0.05).
  
  
  CONCLUSIONS
  LPCA and embryo VM existed in embryonic vasculogenesis. The relative protein of HIF-1α regulated the mouse embryonic vasculogenesis.
authors.affiliation: Department of Pathology, Tianjin Medical University, Tianjin, 300070 China ; Department of Pathology, Tianjin General Hospital, Tianjin Medical University, Tianjin, 300052 China.
authors.forename: Xueyi
authors.initials: X
authors.lastname: Dong
authors.affiliation: Department of Pathology, Tianjin Medical University, Tianjin, 300070 China ; Department of Pathology, Tianjin Cancer Hospital, Tianjin Medical University, Tianjin, 300060 China ; Department of Pathology, Tianjin General Hospital, Tianjin Medical University, Tianjin, 300052 China.
authors.forename: Baocun
authors.initials: B
authors.lastname: Sun
authors.affiliation: Department of Pathology, Tianjin Medical University, Tianjin, 300070 China ; Department of Pathology, Tianjin General Hospital, Tianjin Medical University, Tianjin, 300052 China.
authors.forename: Xiulan
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Pathology, Tianjin Cancer Hospital, Tianjin Medical University, Tianjin, 300060 China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Pathology, Tianjin Medical University, Tianjin, 300070 China ; Department of Pathology, Tianjin General Hospital, Tianjin Medical University, Tianjin, 300052 China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Gu
authors.affiliation: Department of Pathology, Tianjin Medical University, Tianjin, 300070 China ; Department of Pathology, Tianjin General Hospital, Tianjin Medical University, Tianjin, 300052 China.
authors.forename: Danfang
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Tianjin Medical University, Tianjin, 300070 China ; Department of Pathology, Tianjin General Hospital, Tianjin Medical University, Tianjin, 300052 China.
authors.forename: Nan
authors.initials: N
authors.lastname: Zhao
authors.affiliation: Department of Pathology, Tianjin Medical University, Tianjin, 300070 China ; Department of Pathology, Tianjin General Hospital, Tianjin Medical University, Tianjin, 300052 China.
authors.forename: Jinjing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Pathology, Tianjin Medical University, Tianjin, 300070 China.
authors.forename: Jiadong
authors.initials: J
authors.lastname: Chi
keywords: Embryo vasculogenic mimicry; Hypoxia; Hypoxia-inducible factor-1α; Linearly patterned cell apoptosis