title: Genome analysis of Excretory/Secretory proteins in Taenia solium reveals their Abundance of Antigenic Regions (AAR).
abstract: Excretory/Secretory (ES) proteins play an important role in the host-parasite interactions. Experimental identification of ES proteins is time-consuming and expensive. Alternative bioinformatics approaches are cost-effective and can be used to prioritize the experimental analysis of therapeutic targets for parasitic diseases. Here we predicted and functionally annotated the ES proteins in T. solium genome using an integration of bioinformatics tools. Additionally, we developed a novel measurement to evaluate the potential antigenicity of T. solium secretome using sequence length and number of antigenic regions of ES proteins. This measurement was formalized as the Abundance of Antigenic Regions (AAR) value. AAR value for secretome showed a similar value to that obtained for a set of experimentally determined antigenic proteins and was different to the calculated value for the non-ES proteins of T. solium genome. Furthermore, we calculated the AAR values for known helminth secretomes and they were similar to that obtained for T. solium. The results reveal the utility of AAR value as a novel genomic measurement to evaluate the potential antigenicity of secretomes. This comprehensive analysis of T. solium secretome provides functional information for future experimental studies, including the identification of novel ES proteins of therapeutic, diagnosis and immunological interest.
authors.affiliation: Instituto Nacional de Neurología y Neurocirugía, México, D.F., C.P. 14269, México.
authors.forename: Sandra
authors.initials: S
authors.lastname: Gomez
authors.affiliation: 1] Instituto Nacional de Neurología y Neurocirugía, México, D.F., C.P. 14269, México [2] Unidad Periférica del Instituto de Investigaciones Biomédicas en el Instituto Nacional de Neurología y Neurocirugía, México, D.F., C.P. 14269, México.
authors.forename: Laura
authors.initials: L
authors.lastname: Adalid-Peralta
authors.affiliation: Instituto Nacional de Neurología y Neurocirugía, México, D.F., C.P. 14269, México.
authors.forename: Hector
authors.initials: H
authors.lastname: Palafox-Fonseca
authors.affiliation: Instituto Nacional de Medicina Genómica, Periférico Sur No. 4809, Col. Arenal Tepepan, Delegación Tlalpan, México, D.F. C.P. 14610, México.
authors.forename: Vito Adrian
authors.initials: VA
authors.lastname: Cantu-Robles
authors.affiliation: 1] Instituto Nacional de Medicina Genómica, Periférico Sur No. 4809, Col. Arenal Tepepan, Delegación Tlalpan, México, D.F. C.P. 14610, México [2] Instituto de Biotecnología, Universidad Nacional Autónoma de México, Avenida Universidad 2001, Cuernavaca, Morelos, C.P. 62210, México.
authors.forename: Xavier
authors.initials: X
authors.lastname: Soberón
authors.affiliation: Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México, D.F., C.P. 04510, México.
authors.forename: Edda
authors.initials: E
authors.lastname: Sciutto
authors.affiliation: Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México, D.F., C.P. 04510, México.
authors.forename: Gladis
authors.initials: G
authors.lastname: Fragoso
authors.affiliation: Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México, D.F., C.P. 04510, México.
authors.forename: Raúl J
authors.initials: RJ
authors.lastname: Bobes
authors.affiliation: Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México, D.F., C.P. 04510, México.
authors.forename: Juan P
authors.initials: JP
authors.lastname: Laclette
authors.affiliation: Instituto Nacional de Medicina Genómica, Periférico Sur No. 4809, Col. Arenal Tepepan, Delegación Tlalpan, México, D.F. C.P. 14610, México.
authors.forename: Luis del Pozo
authors.initials: Ldel P
authors.lastname: Yauner
authors.affiliation: Unidad de Genómica de Poblaciones Aplicada a la Salud, Facultad de Química, UNAM-Instituto Nacional de Medicina Genómica (INMEGEN), Periférico Sur No. 4809, Col. Arenal Tepepan, Delegación Tlalpan México, D.F. C.P. 14610, México.
authors.forename: Adrián
authors.initials: A
authors.lastname: Ochoa-Leyva
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics* / Q000276:immunology; D019295:Computational Biology; D023281:Genomics; D015801:Helminth Proteins / Q000235:genetics* / Q000276:immunology; D058977:Molecular Sequence Annotation; D020543:Proteome; D040901:Proteomics; D041201:Taenia solium / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism
chemical_list: D000947:Antigens, Helminth; D015801:Helminth Proteins; D020543:Proteome