title: In vivo effects of UV radiation on multiple endpoints and expression profiles of DNA repair and heat shock protein (Hsp) genes in the cycloid copepod Paracyclopina nana.
abstract: To evaluate the effects of ultraviolet (UV) radiation on energy acquisition and consumption, the copepod Paracyclopina nana was irradiated with several doses (0-3kJ/m(2)) of UV. After UV radiation, we measured the re-brooding success, growth pattern of newly hatched nauplii, ingestion rate, and assimilation of diet. In addition, we checked the modulated patterns of DNA repair and heat shock protein (hsp) chaperoning genes of P. nana. UV-B radiation induced a significant reduction (7-87%) of the re-brooding rate of ovigerous females, indicating that UV-induced egg sac damage is closely correlated with a reduction in the hatching rate of UV-irradiated ovigerous female offspring. Using chlorophyll a and stable carbon isotope incubation experiments, we found a dose-dependent decrease (P<0.05) in food ingestion and the rate of assimilation to the body in response to UV radiation, implying that P. nana has an underlying ability to shift its balanced-energy status from growth and reproduction to DNA repair and adaptation. Also, expression of P. nana base excision repair (BER)-associated genes and hsp chaperoning genes was significantly increased in response to UV radiation in P. nana. These findings indicate that even 1kJ/m(2) of UV radiation induces a reduction in reproduction and growth patterns, alters the physiological balance and inhibits the ability to cope with UV-induced damage in P. nana.
authors.affiliation: Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 440-746, South Korea.
authors.forename: Eun-Ji
authors.initials: EJ
authors.lastname: Won
authors.affiliation: Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 440-746, South Korea.
authors.forename: Jeonghoon
authors.initials: J
authors.lastname: Han
authors.affiliation: Department of Marine Sciences and Convergent Technology, College of Science and Technology, Hanyang University, Ansan 426-791, South Korea.
authors.forename: Yeonjung
authors.initials: Y
authors.lastname: Lee
authors.affiliation: Department of Marine Sciences and Convergent Technology, College of Science and Technology, Hanyang University, Ansan 426-791, South Korea.
authors.forename: K Suresh
authors.initials: KS
authors.lastname: Kumar
authors.affiliation: Department of Marine Sciences and Convergent Technology, College of Science and Technology, Hanyang University, Ansan 426-791, South Korea.
authors.forename: Kyung-Hoon
authors.initials: KH
authors.lastname: Shin
authors.affiliation: Department of Life Sciences, College of Natural Sciences, Hanyang University, Seoul 133-791, South Korea.
authors.forename: Su-Jae
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Department of Marine Resource Development, College of Life Sciences, Gangneung-Wonju National University, Gangneung 210-702, South Korea. Electronic address: hgpark@gwnu.ac.kr.
authors.forename: Heum Gi
authors.initials: HG
authors.lastname: Park
authors.affiliation: Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 440-746, South Korea. Electronic address: jslee2@skku.edu.
authors.forename: Jae-Seong
authors.initials: JS
authors.lastname: Lee
mesh_terms: D000818:Animals; D033342:Copepoda / Q000235:genetics / Q000528:radiation effects*; D004260:DNA Repair / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation / Q000528:radiation effects*; D006360:Heat-Shock Proteins / Q000235:genetics*; D012098:Reproduction / Q000528:radiation effects; D014466:Ultraviolet Rays; D014875:Water Pollutants, Radioactive / Q000633:toxicity*
chemical_list: D006360:Heat-Shock Proteins; D014875:Water Pollutants, Radioactive
keywords: Copepod Paracyclopina nana; DNA repair; Heat shock protein (Hsp); Multiple endpoints; Stable isotope; UV-radiation