title: Is the cellular initiation of translation an exclusive property of the initiator tRNAs?
abstract: Translation of mRNAs is the primary function of the ribosomal machinery. Although cells allow for a certain level of translational errors/mistranslation (which may well be a strategic need), maintenance of the fidelity of translation is vital for the cellular function and fitness. The P-site bound initiator tRNA selects the start codon in an mRNA and specifies the reading frame. A direct P-site binding of the initiator tRNA is a function of its special structural features, ribosomal elements, and the initiation factors. A highly conserved feature of the 3 consecutive G:C base pairs (3 GC pairs) in the anticodon stem of the initiator tRNAs is vital in directing it to the P-site. Mutations in the 3 GC pairs diminish/abolish initiation under normal physiological conditions. Using molecular genetics approaches, we have identified conditions that allow initiation with the mutant tRNAs in Escherichia coli. During our studies, we have uncovered a novel phenomenon of in vivo initiation by elongator tRNAs. Here, we recapitulate how the cellular abundance of the initiator tRNA, and nucleoside modifications in rRNA are connected with the tRNA selection in the P-site. We then discuss our recent finding of how a conserved feature in the mRNA, the Shine-Dalgarno sequence, influences tRNA selection in the P-site.
authors.affiliation: a Department of Microbiology and Cell Biology; Indian Institute of Science ; Bangalore , India.
authors.forename: Sunil
authors.initials: S
authors.lastname: Shetty
authors.forename: Souvik
authors.initials: S
authors.lastname: Bhattacharyya
authors.forename: Umesh
authors.initials: U
authors.lastname: Varshney
mesh_terms: D003593:Cytoplasm / Q000378:metabolism*; D004926:Escherichia coli; D010442:Peptide Chain Initiation, Translational; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism; D012358:RNA, Transfer, Met / Q000378:metabolism*; D012270:Ribosomes / Q000737:chemistry / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D012358:RNA, Transfer, Met
keywords: 3GC base pairs; 70S initiation; SD; Shine-Dalgarno; aSD; anti-Shine-Dalgarno; initiation with elongator tRNAs; proteome diversity/plasticity; ribosomal heterogeneity