title: Over-expression of recombinant proteins with N-terminal His-tag via subcellular uneven distribution in Escherichia coli.
abstract: Specific tags with defined amino acid residues are widely used to purify or probe target proteins. Interestingly, the tagging system occasionally results in an increase of the recombinant protein expression in vivo. Here, we systematically examined this phenomenon using a poly-histidine (His)-tag fused to N- or C-terminal region of green, red, and blue fluorescent proteins by quantification and uneven distribution in cytoplasm of Escherichia coli. This effect was further supported by the distinct over-expression of several unrelated proteins, such as esterase, neopullulanase, and chloramphenicol acetyltransferase, tagging with the same tag. These results suggest that a poly-His-tag placed at N-terminal region can induce over-expression of recombinant protein via subcellular uneven distribution in vivo.
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Won-Ji
authors.initials: WJ
authors.lastname: Park
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Sung-Hwan
authors.initials: SH
authors.lastname: You
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Hyoung-An
authors.initials: HA
authors.lastname: Choi
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Yeon-Jin
authors.initials: YJ
authors.lastname: Chu
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea gjkim@chonnam.ac.kr.
authors.forename: Geun-Joong
authors.initials: GJ
authors.lastname: Kim
mesh_terms: D004926:Escherichia coli / Q000378:metabolism*; D006639:Histidine / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics; D013347:Subcellular Fractions / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D006639:Histidine
keywords: His-tag; immunofluorescence; over-expression; uneven distribution