title: Continuous expression of CD83 on activated human CD4⁺ T cells is correlated with their differentiation into induced regulatory T cells.
abstract: CD83 is a widely recognized surface marker for mature dendritic cells, which are essential for priming naïve CD4+ T cells into effector cells. However, CD83 is also expressed on activated CD4+ T cells, which remains an enigma in T‑cell mediated immunity. Therefore, the identification of the biological features and regulation of the expression of CD83 on activated CD4+ T cells is important in understanding the function of CD83 in the adaptive immune response. The present study revealed a time‑dependent manner of the expression of CD83 on anti‑CD3/CD28‑stimulated human CD4+ T cells, which is characterized by the maximum expression at day 2 and a significant decrease at day 3. The reduced expression is not a result of a reduced rate of cell proliferation. The activation of interleukin‑2 and secretion of interferon‑γ accumulated progressively from day 1 to 3. Of note, sustained expression of CD83 was observed when CD4+ T cells were induced by transforming growth factor-β to differentiate into CD4+CD25+ forkhead box P3+ regulatory T (iTreg) cells. Confocal immunofluorescence microscopy analysis demonstrated that CD83 was highly co‑localized with CD25 on activated CD4+ T cells. In conclusion, the findings of the present study suggested that the continuous expression of CD83 on activated human CD4+ T cells is correlated with their differentiation into iTreg cells.
authors.affiliation: Department of Laboratory Medicine, The Second Hospital of Anhui Medical University, Hefei, Anhui 230601, P.R. China.
authors.forename: Liwen
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Laboratory Medicine, The Second Hospital of Anhui Medical University, Hefei, Anhui 230601, P.R. China.
authors.forename: Shihe
authors.initials: S
authors.lastname: Guan
authors.affiliation: Department of Laboratory Medicine, The Second Hospital of Anhui Medical University, Hefei, Anhui 230601, P.R. China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Department of Medical Research Center, The Second Hospital of Anhui Medical University, Hefei, Anhui 230601, P.R. China.
authors.forename: Shouqin
authors.initials: S
authors.lastname: Sheng
authors.affiliation: Department of Medical Oncology, The First Hospital of Anhui Medical University, Hefei, Anhui 230022, P.R. China.
authors.forename: Fei
authors.initials: F
authors.lastname: Zhong
authors.affiliation: Department of Laboratory Medicine, The Second Hospital of Anhui Medical University, Hefei, Anhui 230601, P.R. China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
mesh_terms: D015703:Antigens, CD / Q000032:analysis / Q000276:immunology*; D015704:CD4 Antigens / Q000032:analysis / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis / Q000276:immunology*; D053645:Interleukin-2 Receptor alpha Subunit / Q000032:analysis / Q000276:immunology; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000032:analysis / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000166:cytology / Q000276:immunology*; D000097802:CD83 Antigen
chemical_list: D015703:Antigens, CD; D015704:CD4 Antigens; D007136:Immunoglobulins; D053645:Interleukin-2 Receptor alpha Subunit; D008562:Membrane Glycoproteins; D000097802:CD83 Antigen