title: Identification of chemical constituents and rat metabolites of Kangxianling granule by HPLC-Q-TOF-MS/MS.
abstract: A high-performance liquid chromatography coupled with quadrupole time-of-flight mass tandem mass spectrometry method was established to characterize the chemical constituents of Kangxianling granule (KXL), a traditional Chinese medicine formula, and the metabolic profile in rat urine and plasma after oral administration of KXL. A total of 27 compounds in KXL extract and 13 prototype compounds with 12 metabolites in rat urine and plasma were identified. Among the 27 detected compounds, 15 were identified by comparing the retention time and MS data with that of reference compounds and the other 12 compounds were tentatively assigned based on the MS data and reference literature. The main prototype components absorbed in rat were amygdalin, salvianolic acid B, tanshinones and anthraquinones. Hydroxylation, glucuronidation and sulfation were the principal metabolic pathways in rat. The results revealed that the 25 compounds identified in rat urine and plasma were the potential active ingredients of KXL, which provides helpful chemical information for further study of the pharmacology mechanism of KXL.
authors.affiliation: Yangtze River Pharmaceutical Group Co. Ltd, Taizhou, 225321, People's Republic of China.|Shanghai Haini Pharmaceutical Co. Ltd, Yangtze River Pharmaceutical Group, Shanghai, 201318, People's Republic of China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Shanghai Haini Pharmaceutical Co. Ltd, Yangtze River Pharmaceutical Group, Shanghai, 201318, People's Republic of China.
authors.forename: Lianxiang
authors.initials: L
authors.lastname: Fang
authors.affiliation: Shanghai Haini Pharmaceutical Co. Ltd, Yangtze River Pharmaceutical Group, Shanghai, 201318, People's Republic of China.
authors.forename: Haijian
authors.initials: H
authors.lastname: Cong
authors.affiliation: Yangtze River Pharmaceutical Group Co. Ltd, Taizhou, 225321, People's Republic of China.
authors.forename: Ting
authors.initials: T
authors.lastname: Xiang
authors.affiliation: Yangtze River Pharmaceutical Group Co. Ltd, Taizhou, 225321, People's Republic of China.
authors.forename: Ming
authors.initials: M
authors.lastname: Xue
authors.affiliation: Yangtze River Pharmaceutical Group Co. Ltd, Taizhou, 225321, People's Republic of China.
authors.forename: Zhongqing
authors.initials: Z
authors.lastname: Yao
authors.affiliation: Yangtze River Pharmaceutical Group Co. Ltd, Taizhou, 225321, People's Republic of China.|Shanghai Haini Pharmaceutical Co. Ltd, Yangtze River Pharmaceutical Group, Shanghai, 201318, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
authors.affiliation: School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai, 201418, People's Republic of China.
authors.forename: Wenhui
authors.initials: W
authors.lastname: Lin
mesh_terms: D000818:Animals; D000880:Anthraquinones / Q000097:blood / Q000378:metabolism / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004224:Diterpenes / Q000097:blood / Q000378:metabolism / Q000652:urine; D004365:Drugs, Chinese Herbal / Q000378:metabolism*; D006027:Glycosides / Q000097:blood / Q000378:metabolism / Q000652:urine; D062385:Hydroxybenzoates / Q000097:blood / Q000378:metabolism / Q000652:urine; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000880:Anthraquinones; D004224:Diterpenes; D004365:Drugs, Chinese Herbal; D006027:Glycosides; D062385:Hydroxybenzoates; C533464:kangxianling; C017616:phenolic acid
keywords: HPLC-Q-TOF-MS/MS; Kangxianling granule; chemical constituents; rats metabolites