title: Sterilization of polydimethylsiloxane surface with Chinese herb extract: a new antibiotic mechanism of chlorogenic acid.
abstract: Coating of polydimethylsiloxane (PDMS) surface with a traditional Chinese herb extract chlorogenic acid (CA) solves the contemporary problem of sterilization of PDMS surface. The E. coli grows slower and has a higher death rate on the CA-coated PDMS surfaces. A smoother morphology of these E. coli cell wall is observed by atomic force microscopy (AFM). Unlike the reported mechanism, where CA inhibits bacterial growth by damaging the cell membrane in the bulk solution, we find the CA-coated PDMS surface also decreases the stiffness of the cell wall. A decrease in the Young's modulus of the cell wall from 3 to 0.8 MPa is reported. Unexpectedly, the CA effect on the swarming ability and the biofilm stability of the bacteria can be still observed, even after they have been removed from the CA environment, indicating a decrease in their resistance to antibiotics for a prolonged time. The CA-coated PDMS surface shows better antibiotic effect against three types of both Gram-positive and Gran-negative bacteria than the gentamicin-coated PDMS surface. Coating of CA on PDMS surface not only solves the problem of sterilization of PDMS surface, but also shines light on the application of Chinese traditional herbs in scientific research.
authors.affiliation: Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin 300071, China.
authors.forename: Song
authors.initials: S
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Life Science, Nankai University, Tianjin, 300071, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Wu
authors.affiliation: Institute of Robotics and Automatic Information Systems, Nankai University, Tianjin 300071, China.
authors.forename: Jiayu
authors.initials: J
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Life Science, Nankai University, Tianjin, 300071, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Institute of Robotics and Automatic Information Systems, Nankai University, Tianjin 300071, China.
authors.forename: Xiaohan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Institute of Physical Chemistry Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.
authors.forename: Lili
authors.initials: L
authors.lastname: Sun
authors.affiliation: Institute of Physical Chemistry Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.
authors.forename: Robert
authors.initials: R
authors.lastname: Holyst
authors.affiliation: 1] State Key Laboratory of Medicinal Chemical Biology, College of Life Science, Nankai University, Tianjin, 300071, China [2] Institute of Physical Chemistry Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.
authors.forename: Sen
authors.initials: S
authors.lastname: Hou
authors.affiliation: Institute of Robotics and Automatic Information Systems, Nankai University, Tianjin 300071, China.
authors.forename: Yongchun
authors.initials: Y
authors.lastname: Fang
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Life Science, Nankai University, Tianjin, 300071, China.
authors.forename: Xizeng
authors.initials: X
authors.lastname: Feng
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects / Q000502:physiology; D018441:Biofilms / Q000187:drug effects; D002473:Cell Wall / Q000737:chemistry; D002726:Chlorogenic Acid / Q000737:chemistry* / Q000494:pharmacology; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology; D055119:Elastic Modulus; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development / Q000502:physiology; D018625:Microscopy, Atomic Force; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000502:physiology; D013499:Surface Properties
chemical_list: D000900:Anti-Bacterial Agents; D004129:Dimethylpolysiloxanes; D004365:Drugs, Chinese Herbal; D002726:Chlorogenic Acid; C013830:baysilon