title: [Expression of CDK4 and p16 in laryngeal squamous cell carcinoma].
abstract: OBJECTIVE
  To investigate the expression of CDK4 and p16 in laryngeal squamous cell carcinoma (LSCC) tissues.
  
  
  METHODS
  The expressions of CDK4 and p16 in 30 cases of LSCC tissues and 20 cases of edge tissues were detected by immunohistochemical technology SP method, and discuss their correlation with clincial pathology and clinical stage of LSCC.
  
  
  RESULTS
  The positive rates of CDK4 and p16 were 63. 3 %,46. 7% in LSCC tissues, and the positive rates were 25%, 90% in edge tissues. The expression of CDK4 in LSCC tissues was significantly higher than that in edge tissues(P<0. 05), which was not associated with the clincial pathology and clinical stage(P> 0. 05); The expression of p16 in LSCC tissues was significantly lower than that in edge tissues(P<0. 05), it was associated with the clincial pathology (P<0. 05), but not associated with clinical stage(P>0. 05) ;there is a negative correlation between CDK4 and p16 (r= -0. 786, P<0. 05).
  
  
  CONCLUSIONS
  Low expression of p16 and high expression of CDK4 may play an important role in the development of LSCC and the low expression of p16 in LSCC tissue could be used as important reference markers of malignant degree of tumour.
authors.forename: Junxing
authors.initials: J
authors.lastname: Zhang
authors.forename: Manying
authors.initials: M
authors.lastname: Geng
authors.forename: Lei
authors.initials: L
authors.lastname: Su
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Xing
authors.initials: X
authors.lastname: Lu
mesh_terms: D014408:Biomarkers, Tumor; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D051358:Cyclin-Dependent Kinase 4 / Q000096:biosynthesis*; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000096:biosynthesis*; D006258:Head and Neck Neoplasms / Q000378:metabolism*; D007822:Laryngeal Neoplasms / Q000378:metabolism*; D011379:Prognosis; D000077195:Squamous Cell Carcinoma of Head and Neck
chemical_list: D014408:Biomarkers, Tumor; D019941:Cyclin-Dependent Kinase Inhibitor p16; C495900:CDK4 protein, human; D051358:Cyclin-Dependent Kinase 4