title: Various effects of fluorescent bacteria of the genus Pseudomonas containing ACC deaminase on wheat seedling growth.
abstract: The study evaluates the effect of rhizobacteria having 1-aminocyclopropane-1-carboxylate deaminase (ACCd) on the development of wheat seedlings. This enzyme has been proposed to play a key role in microbe-plant association. Three fluorescent pseudomonads containing this deaminase were selected from 70 strains of pseudomonads isolated from rhizosphere of wheat (Triticum aestivum L.) and rape (Brassica napus L.). These bacteria, varied significantly in the ability to both biosynthesize auxins and hydrolyze ACC. Among them, Pseudomonas brassicacearum subsp. brassicacearum strain RZ310 presented the highest activities of ACC deaminase during 96h of growth in liquid Dworkin and Foster (DF) salt medium. Additionally, this rape rhizosphere strain did not produce indoles. Two other isolates, Pseudomonas sp. PO283 and Pseudomonas sp. PO366, secreted auxins only in the presence of their precursor. Phylogenetic analysis of the 16S rRNA gene and four other protein-encoding genes indicated that these wheat rhizosphere isolates belonged to the fluorescent Pseudomonas group. Moreover, the effects of these strains on wheat seedling growth under in vitro conditions were markedly dependent on both their cell suspensions used to grain inoculation and nutrient conditions. Strains tested had beneficial influence on wheat seedlings mainly at low cell densities. In addition, access to nutrients markedly changed bacteria action on cereal growth. Their presence generally favored the positive effects of pseudomonads on length and the estimated biomasses of wheat coleoptiles. Despite these general rules, impacts of each isolate on the growth parameters of cereal seedlings were unique.
authors.affiliation: Agricultural Microbiology Lab, Department of Plant Protection, Wrocław University of Environmental and Life Sciences, Grunwaldzka 53, 50-375 Wrocław, Poland. Electronic address: elzbieta.magnucka@up.wroc.pl.
authors.forename: Elżbieta G
authors.initials: EG
authors.lastname: Magnucka
authors.affiliation: Agricultural Microbiology Lab, Department of Plant Protection, Wrocław University of Environmental and Life Sciences, Grunwaldzka 53, 50-375 Wrocław, Poland.
authors.forename: Stanisław J
authors.initials: SJ
authors.lastname: Pietr
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D001483:Base Sequence; D018533:Biomass; D019755:Carbon-Carbon Lyases / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology; D016133:Polymerase Chain Reaction; D011549:Pseudomonas / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D058441:Rhizosphere; D036226:Seedlings / Q000254:growth & development / Q000382:microbiology*; D012988:Soil Microbiology; D014908:Triticum / Q000254:growth & development / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; C033634:1-aminocyclopropane-1-carboxylate deaminase; D019755:Carbon-Carbon Lyases
keywords: 1-Aminocyclopropane-1-carboxylate deaminase; Auxins; P. brassicacearum subsp. brassicacearum; Pseudomonads; Triticum aestivum L.