title: Antibodies against human platelet alloantigens and human leucocyte antigen class 1 in Saudi Arabian multiparous women and multi-transfused patients.
abstract: OBJECTIVE
  To determine the frequency of alloimmunization against human platelet antigens (HPAs) and human leucocyte antigen class 1 (HLA1) in multiparous women and multi-transfused patients.
  
  
  METHODS
  This prospective study was conducted between January and August 2013, on 50 multiparous women with no history of previous blood transfusion recruited from the Obstetrics and Gynecology Clinic, and 50 patients, who received multiple platelet transfusions, recruited from the Hematology/Oncology Ward, King Khalid University Hospital, Riyadh, Saudi Arabia.
  
  
  RESULTS
  The frequency of alloimmunization among multiparous pregnant women was 76%, as follows: 16% against HLA1 only, 8% against HPAs only, 52% against both HPAs and HLA1 antigens. In multi-transfused patients, the rate of alloimmunization was 42% as follows: 2% against HLA1 only, 22% against HPAs only, 18% against both HPAs and HLA1 antigens. The frequency of alloimmunization increases with the number of pregnancies, but not with the number of platelet transfusions.
  
  
  CONCLUSIONS
  Alloimmunization against HPAs and HLA1 is very common among Saudi multiparous women and multi-transfused patients, which encourages the search for the extent of the possible complications in the fetus and newborn and in multitransfused patients and how to prevent their occurrence.
authors.affiliation: Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Kingdom of Saudi Arabia. E-mail. salouda@ksu.edu.sa.
authors.forename: Sarah K
authors.initials: SK
authors.lastname: Al-Ouda
authors.forename: Abdulmajeed A
authors.initials: AA
authors.lastname: Al-Banyan
authors.forename: Farjah H
authors.initials: FH
authors.lastname: Al-Gahtani
authors.forename: Abdel-Galil M
authors.initials: AG
authors.lastname: Abdel-Gader
authors.forename: Lateefa O
authors.initials: LO
authors.lastname: Al-Dakhil
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D016824:Antigens, Human Platelet / Q000276:immunology*; D018955:CD36 Antigens / Q000276:immunology; D005260:Female; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D006801:Humans; D038982:Integrin alpha2beta1 / Q000276:immunology; D007518:Isoantibodies / Q000276:immunology*; D007519:Isoantigens / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D010298:Parity / Q000276:immunology*; D017713:Platelet Transfusion; D011446:Prospective Studies; D012529:Saudi Arabia; D055815:Young Adult
chemical_list: D016824:Antigens, Human Platelet; D018955:CD36 Antigens; D015395:Histocompatibility Antigens Class I; D038982:Integrin alpha2beta1; D007518:Isoantibodies; D007519:Isoantigens