title: Regulation of Protein Binding Capability of Surfaces via Host-Guest Interactions: Effects of Localized and Average Ligand Density.
abstract: The protein binding capability of biomaterial surfaces can significantly affect subsequent biological responses, and appropriate ligand presentation is often required to guarantee the best functions. Herein, a new facile method for regulating this capability by varying the localized and average ligand density is presented. Binding between lysine and plasminogen relevant to a fibrinolysis system was chosen as a model. We integrated different lysine-modified β-cyclodextrin (β-CD) derivatives onto bioinert copolymer brushes via host-guest interactions. The localized and average lysine density can be conveniently modulated by changing the lysine valency on β-CD scaffolds and by diluting lysine-persubstituted β-CD with pure β-CD, respectively. Both the plasminogen adsorption and the plasminogen binding affinity were enhanced by lysine-persubstituted β-CD compared with those of lysine-monosubstituted β-CD, which is possibly due to the higher localized lysine density and the multivalent binding of plasminogen on lysine-persubstituted β-CD surfaces. With a change in the ratio of lysine-persubstituted β-CD to β-CD, the average lysine density can be tuned, leading to the linear regulation of the adsorption of plasminogen on surfaces.
authors.affiliation: †College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, P. R. China.
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Shi
authors.affiliation: †College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, P. R. China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Zhan
authors.affiliation: †College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, P. R. China.|‡Center for Soft Condensed Matter Physics and Interdisciplinary Research, Soochow University, Suzhou 215006, P. R. China.
authors.forename: Gaojian
authors.initials: G
authors.lastname: Chen
authors.affiliation: †College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, P. R. China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Yu
authors.affiliation: †College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, P. R. China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
authors.affiliation: †College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, P. R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Du
authors.affiliation: †College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, P. R. China.
authors.forename: Limin
authors.initials: L
authors.lastname: Cao
authors.affiliation: †College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, P. R. China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Liu
authors.affiliation: †College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, P. R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Yuan
authors.affiliation: †College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, P. R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
mesh_terms: D001665:Binding Sites; D008024:Ligands; D008239:Lysine / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure; D010958:Plasminogen / Q000737:chemistry / Q000378:metabolism*; D013499:Surface Properties; D047392:beta-Cyclodextrins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D008024:Ligands; D047392:beta-Cyclodextrins; D010958:Plasminogen; C031215:betadex; D008239:Lysine