title: Application of avidin-biotin technology to improve cell adhesion on nanofibrous matrices.
abstract: BACKGROUND
  Electrospinning is an easy and effective technique to produce submicron fibers possessing a range of attractive characteristics such as interconnected porous structures similar to natural ECM and good resilience to movement. Rapid and efficient cell attachment to nanofibrous matrices is a necessary prerequisite in tissue engineering. Thus, the aim of this study is to evaluate poly(ε-caprolactone-co-lactide)/Pluronic (PLCL/Pluronic) nanofibrous matrices with avidin-biotin technology for improving cell adhesion for the first time.
  
  
  RESULTS
  PLCL/Pluronic nanofibers had relatively homogeneous fibers and interconnected porous structures. Pluronic significantly modified the hydrophilicity of nanofibrous matrices and PLCL/Pluronic nanofibrous matrices had better performance on maintaining cell proliferation. Avidin-biotin technology had no negative effect on the hydrophilic property, mechanical property and cell proliferation. Meanwhile, the attachment and spreading of adipose-derived stem cells (ADSCs) onto PLCL/Pluronic nanofibrous matrices with avidin-biotin technology was promoted obviously.
  
  
  CONCLUSIONS
  PLCL/Pluronic nanofibrous matrices inheriting the excellent characteristics of both PLCL and Pluronic have the better cell adhesion ability through avidin-biotin technology, implying a promising application in skin care, tissue regeneration and other related area.
authors.affiliation: Department of Orthopaedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 YiShan Road, Shanghai, 200233, China. pansmith@163.com.
authors.forename: Jian-feng
authors.initials: JF
authors.lastname: Pan
authors.affiliation: Department of Orthopaedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 YiShan Road, Shanghai, 200233, China. lnhdoc@126.com.
authors.forename: Ning-hua
authors.initials: NH
authors.lastname: Liu
authors.affiliation: Department of Emergency, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 YiShan Road, Shanghai, 200233, China. 13661549752@163.com.
authors.forename: Lin-yuan
authors.initials: LY
authors.lastname: Shu
authors.affiliation: Department of Orthopaedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 YiShan Road, Shanghai, 200233, China. sunshine20002000@126.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Sun
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D001360:Avidin / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry*; D001710:Biotin / Q000737:chemistry*; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D020442:Poloxamer / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D017207:Rats, Sprague-Dawley; D017768:Skin, Artificial; D013234:Stem Cells / Q000166:cytology*; D013314:Stress, Mechanical; D013718:Tensile Strength; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; C505727:poly(epsilon-caprolactone-co-lactide); D014867:Water; D020442:Poloxamer; D001360:Avidin; D001710:Biotin