title: [Cloning and functional characterization of pathogenesis-related PR10-1 gene in Panax notoginseng].
abstract: With homology cloning approaches coupling with RACE (rapid-amplification of cDNA ends) techniques, the full-length coding sequence of pathogenesis-related protein PR10-1 with differential expression was cloned from the total RNA of the root of Panax notoginseng, and its function was explored furtherly. As a result, the longest 465 bp ORF (named as PnPR10-1 with the Accession No. KJ741402 in GenBank) was detected from the cloned sequence with full-length of cDNA of 863 bp. The corresponding peptide encoded consisted of 155 amino acids, contained some domains such as Bet-v-I, and showed high similarity with that from Panax ginseng by analysis of phylogenetic trees created from the alignments. Real-time quantitative PCR showed that the expression of PnPR10-1 gene was constitutive in different tissues of 1-3 year old plant, suggesting that it might be involved in growth, development, and secondary metabolism; yet it was up-regulated significantly with the infection of Fusarium oxysporum in root, suggesting that it might be involved in defense against many diseases including root rot in P. notoginseng.
authors.forename: Mei-Qiong
authors.initials: MQ
authors.lastname: Tang
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Min
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.forename: Ni
authors.initials: N
authors.lastname: Jiang
authors.forename: Yun-Feng
authors.initials: YF
authors.lastname: Ye
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D017343:Genes, Plant; D006026:Glycoside Hydrolases / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D053723:Panax notoginseng / Q000235:genetics*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*; D018517:Plant Roots
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins; C053376:pathogenesis-related proteins, plant; D006026:Glycoside Hydrolases