title: Retrograde Trafficking Inhibitor of Shiga Toxins Reduces Morbidity and Mortality of Mice Infected with Enterohemorrhagic Escherichia coli.
abstract: The most deadly outbreak of Escherichia coli O104:H4 occurred in Europe in 2011. Here, we evaluated the effects of the retrograde trafficking inhibitor Retro-2(cycl) in a murine model of E. coli O104:H4 infection. Systemic treatment with Retro-2(cycl) significantly reduced body weight loss and improved clinical scores and survival rates for O104:H4-infected mice. The present data established that Retro-2(cycl) contributes to the protection of mice against O104:H4 infection and may represent a novel approach to limit Shiga toxin-producing Escherichia coli (STEC)-induced toxicity.
authors.affiliation: INRA, USC 1360, Toulouse, France INSERM, UMR 1043, Toulouse, France CNRS, UMR 5282, Toulouse, France Université de Toulouse, Université Paul Sabatier, Centre de Physiopathologie Toulouse Purpan, Toulouse, France.
authors.forename: T
authors.initials: T
authors.lastname: Secher
authors.affiliation: INRA, USC 1360, Toulouse, France INSERM, UMR 1043, Toulouse, France CNRS, UMR 5282, Toulouse, France Université de Toulouse, Université Paul Sabatier, Centre de Physiopathologie Toulouse Purpan, Toulouse, France.
authors.forename: A
authors.initials: A
authors.lastname: Shima
authors.affiliation: CEA, iBiTecS, Service de Chimie Bioorganique et de Marquage, LabEx LERMIT, Gif-sur-Yvette, France.
authors.forename: K
authors.initials: K
authors.lastname: Hinsinger
authors.affiliation: CEA, iBiTecS, Service de Chimie Bioorganique et de Marquage, LabEx LERMIT, Gif-sur-Yvette, France.
authors.forename: J C
authors.initials: JC
authors.lastname: Cintrat
authors.affiliation: Institut Curie, Centre de Recherche, Endocytic Trafficking and Therapeutic Delivery Group, Paris, France CNRS UMR 3666, Paris, France U1143 INSERM, Paris, France.
authors.forename: L
authors.initials: L
authors.lastname: Johannes
authors.affiliation: CEA, iBiTecS, Service d'Ingénierie Moléculaire des Protéines, LabEx LERMIT, Gif-sur-Yvette, France.
authors.forename: J
authors.initials: J
authors.lastname: Barbier
authors.affiliation: CEA, iBiTecS, Service d'Ingénierie Moléculaire des Protéines, LabEx LERMIT, Gif-sur-Yvette, France.
authors.forename: D
authors.initials: D
authors.lastname: Gillet
authors.affiliation: INRA, USC 1360, Toulouse, France INSERM, UMR 1043, Toulouse, France CNRS, UMR 5282, Toulouse, France Université de Toulouse, Université Paul Sabatier, Centre de Physiopathologie Toulouse Purpan, Toulouse, France CHU Toulouse, Hôpital Purpan, Service de Bactériologie-Hygiène, Toulouse, France eric.oswald@inserm.fr.
authors.forename: E
authors.initials: E
authors.lastname: Oswald
mesh_terms: D000818:Animals; D001549:Benzamides / Q000494:pharmacology* / Q000627:therapeutic use; D002522:Chlorocebus aethiops; D004195:Disease Models, Animal; D004196:Disease Outbreaks; D054324:Enterohemorrhagic Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000188:drug therapy* / Q000453:epidemiology; D005060:Europe; D006367:HeLa Cells; D006463:Hemolytic-Uremic Syndrome / Q000188:drug therapy* / Q000517:prevention & control; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D022641:Shiga Toxin 2 / Q000037:antagonists & inhibitors*; D013876:Thiophenes / Q000494:pharmacology* / Q000627:therapeutic use; D014709:Vero Cells
chemical_list: C549073:2-(((5-methyl-2-thienyl)methylene)amino)-N-phenylbenzamide; D001549:Benzamides; D022641:Shiga Toxin 2; D013876:Thiophenes