title: Involvement of the CasK/R two-component system in optimal unsaturation of the Bacillus cereus fatty acids during low-temperature growth.
abstract: Bacillus cereus sensu lato is composed of a set of ubiquitous strains including human pathogens that can survive a range of food processing conditions, grow in refrigerated food, and sometimes cause food poisoning. We previously identified the two-component system CasK/R that plays a key role in cold adaptation. To better understand the CasK/R-controlled mechanisms that support low-temperature adaptation, we performed a transcriptomic analysis on the ATCC 14579 strain and its isogenic ∆casK/R mutant grown at 12°C. Several genes involved in fatty acid (FA) metabolism were downregulated in the mutant, including desA and desB encoding FA acyl-lipid desaturases that catalyze the formation of a double-bond on the FA chain in positions ∆5 and ∆10, respectively. A lower proportion of FAs presumably unsaturated by DesA was observed in the ΔcasK/R strain compared to the parental strain while no difference was found for FAs presumably unsaturated by DesB. Addition of phospholipids from egg yolk lecithin rich in unsaturated FAs, to growth medium, abolished the cold-growth impairment of ΔcasK/R suggesting that exogenous unsaturated FAs can support membrane-level modifications and thus compensate for the decreased production of these FAs in the B. cereus ∆casK/R mutant during growth at low temperature. Our findings indicate that CasK/R is involved in the regulation of FA metabolism, and is necessary for cold adaptation of B. cereus unless an exogenous source of unsaturated FAs is available.
authors.affiliation: INRA, UMR408 Sécurité et Qualité des Produits d'Origine Végétale, 84000 Avignon, France; Université d'Avignon, UMR408 Sécurité et Qualité des Produits d'Origine Végétale, 84000 Avignon, France.
authors.forename: Sara Esther
authors.initials: SE
authors.lastname: Diomandé
authors.affiliation: INRA, UMR408 Sécurité et Qualité des Produits d'Origine Végétale, 84000 Avignon, France; Université d'Avignon, UMR408 Sécurité et Qualité des Produits d'Origine Végétale, 84000 Avignon, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Nguyen-the
authors.affiliation: Top Institute Food and Nutrition, NieuweKanaal 9A, 6709 PA, Wageningen, The Netherlands; Food Microbiology Laboratory, Wageningen University, BornseWeilanden 9, 6708 WG, Wageningen, The Netherlands.
authors.forename: Tjakko
authors.initials: T
authors.lastname: Abee
authors.affiliation: Food Microbiology Laboratory, Wageningen University, BornseWeilanden 9, 6708 WG, Wageningen, The Netherlands.
authors.forename: Marcel H
authors.initials: MH
authors.lastname: Tempelaars
authors.affiliation: INRA, UMR408 Sécurité et Qualité des Produits d'Origine Végétale, 84000 Avignon, France; Université d'Avignon, UMR408 Sécurité et Qualité des Produits d'Origine Végétale, 84000 Avignon, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Broussolle
authors.affiliation: INRA, UMR408 Sécurité et Qualité des Produits d'Origine Végétale, 84000 Avignon, France; Université d'Avignon, UMR408 Sécurité et Qualité des Produits d'Origine Végétale, 84000 Avignon, France; INRA, Université Montpellier, UMR1333 Diversité Génomes et Interactions Microorganismes-Insectes (DGIMI), Montpellier, France. Electronic address: julien.brillard@avignon.inra.fr.
authors.forename: Julien
authors.initials: J
authors.lastname: Brillard
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D001409:Bacillus cereus / Q000201:enzymology* / Q000235:genetics; D003080:Cold Temperature; D003470:Culture Media / Q000737:chemistry; D044943:Fatty Acid Desaturases / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis / Q000378:metabolism*; D005516:Food Microbiology; D005517:Foodborne Diseases / Q000382:microbiology*; D020869:Gene Expression Profiling; D051528:Guanylate Kinases / Q000235:genetics / Q000502:physiology*; D006801:Humans; D010743:Phospholipids / Q000378:metabolism; D044169:Receptors, Calcium-Sensing / Q000235:genetics / Q000502:physiology*
chemical_list: D003470:Culture Media; D005227:Fatty Acids; D010743:Phospholipids; D044169:Receptors, Calcium-Sensing; D044943:Fatty Acid Desaturases; C106868:CASK kinases; D051528:Guanylate Kinases
keywords: B. cereus; Cold adaptation; Desaturase; TCS; Unsaturated FA