title: Native Liquid Extraction Surface Analysis Mass Spectrometry: Analysis of Noncovalent Protein Complexes Directly from Dried Substrates.
abstract: Liquid extraction surface analysis (LESA) mass spectrometry is a promising tool for the analysis of intact proteins from biological substrates. Here, we demonstrate native LESA mass spectrometry of noncovalent protein complexes of myoglobin and hemoglobin from a range of surfaces. Holomyoglobin, in which apomyoglobin is noncovalently bound to the prosthetic heme group, was observed following LESA mass spectrometry of myoglobin dried onto glass and polyvinylidene fluoride surfaces. Tetrameric hemoglobin [(αβ)2(4H)] was observed following LESA mass spectrometry of hemoglobin dried onto glass and polyvinylidene fluoride (PVDF) surfaces, and from dried blood spots (DBS) on filter paper. Heme-bound dimers and monomers were also observed. The 'contact' LESA approach was particularly suitable for the analysis of hemoglobin tetramers from DBS.
authors.affiliation: School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Martin
authors.forename: Rian L
authors.initials: RL
authors.lastname: Griffiths
authors.forename: Rebecca L
authors.initials: RL
authors.lastname: Edwards
authors.forename: Helen J
authors.initials: HJ
authors.lastname: Cooper
mesh_terms: D000328:Adult; D001798:Blood Proteins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D059788:Dried Blood Spot Testing / Q000379:methods*; D005260:Female; D005898:Glass; D006801:Humans; D059625:Liquid-Liquid Extraction / Q000379:methods*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D011145:Polyvinyls; D005465:Fluorocarbon Polymers
chemical_list: D001798:Blood Proteins; D011145:Polyvinyls; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers