title: Methods for Derivation of Multipotent Neural Crest Cells Derived from Human Pluripotent Stem Cells.
abstract: Multipotent, neural crest cells (NCCs) produce a wide range of cell types during embryonic development. This includes melanocytes, peripheral neurons, smooth muscle cells, osteocytes, chondrocytes, and adipocytes. The protocol described here allows for highly efficient differentiation of human pluripotent stem cells to a neural crest fate within 15 days. This is accomplished under feeder-free conditions, using chemically defined medium supplemented with two small molecule inhibitors that block glycogen synthase kinase 3 (GSK3) and bone morphogenic protein (BMP) signaling. This technology is well suited as a platform to understand in greater detail the pathogenesis of human disease associated with impaired neural crest development/migration.
authors.affiliation: Department of Biochemistry and Molecular Biology, Center for Molecular Medicine, Paul D. Coverdell Center for Biomedical and Health Sciences, University of Georgia, 500 DW Brooks Drive, Athens, GA, 30602, USA.
authors.forename: John
authors.initials: J
authors.lastname: Avery
authors.affiliation: Department of Biochemistry and Molecular Biology, Center for Molecular Medicine, Paul D. Coverdell Center for Biomedical and Health Sciences, University of Georgia, 500 DW Brooks Drive, Athens, GA, 30602, USA. sdalton@uga.edu.
authors.forename: Stephen
authors.initials: S
authors.lastname: Dalton
mesh_terms: D019485:Bone Morphogenetic Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D038362:Glycogen Synthase Kinase 3 / Q000037:antagonists & inhibitors / Q000378:metabolism; D000066449:Human Embryonic Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D009432:Neural Crest / Q000166:cytology*; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D019485:Bone Morphogenetic Proteins; D003470:Culture Media; D038362:Glycogen Synthase Kinase 3
keywords: Human embryonic stem cells; Human induced pluripotent stem cells; Human pluripotent stem cells; Neural crest cells