title: High dose of epigallocatechin-3-gallate inhibits proliferation and induces apoptosis of H9C2 cardiomyocytes through down-regulation of SIRT1.
abstract: BACKGROUND
  Previous studies have suggested that high doses of (-)-epigallocatechin-3-gallate (EGCG) can induce toxicity in the liver, kidneys, and intestine. However, there have been no reports of myocardiotoxicity following treatment with EGCG. In this study, we investiged the proliferation and apoptosis of H9C2 cardiomyocytes treated with high dose of EGCG.
  
  
  METHODS
  Cell proliferation was measured by CCK8 assay, cell apoptosis rate was evaluated by TUNEL assay, and the expression alterations of Sirtuin 1 (SIRT1) protein was detected by Western blotting.
  
  
  RESULTS
  EGCG inhibits proliferation and induces apoptosis in time- and dose-dependent manner in H9C2 cardiomyocytes. SIRT1 participates in the inhibitory effect of EGCG on cell proliferation and apoptosis induction in H9C2 cardiomyocytes.
  
  
  CONCLUSIONS
  This study demonstrates that high doses of EGCG inhibit proliferation and induce apoptosis in H9C2 cardiomyocytes. Down-regulation of SIRT 1 protein expression may be involved.
authors.forename: Yi
authors.initials: Y
authors.lastname: Cai
authors.forename: Li
authors.initials: L
authors.lastname: Zhao
authors.forename: Yuan
authors.initials: Y
authors.lastname: Qin
authors.forename: Yanhuan
authors.initials: Y
authors.lastname: He
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D020287:In Situ Nick-End Labeling; D032383:Myocytes, Cardiac / Q000187:drug effects*; D051381:Rats; D056564:Sirtuin 1 / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D000975:Antioxidants; D002392:Catechin; C045651:epigallocatechin gallate; C488368:Sirt1 protein, rat; D056564:Sirtuin 1