title: Pichia dushanensis sp. nov. and Hyphopichia paragotoi sp. nov., two sexual yeast species associated with insects and rotten wood.
abstract: Seven yeast strains were isolated from the gut of insect larvae and decayed wood, which were collected from three localities near Nanyang, Henan Province, China. These strains were identified as two novel species through comparison of sequences in the D1/D2 domains of the large subunit (LSU) rRNA gene and other taxonomic characteristics. Pichia dushanensis sp. nov. was closely related to species in the Pichia clade and produced one to four spheroid ascospores in a deliquescent ascus. The D1/D2 sequence of P. dushanensis sp. nov. differed from its closest relative, Issatchenkia (Pichia) sp. NRRL Y-12824, by 3.6% sequence divergence (16 substitutions and 4 gaps). The species also differed from its four closest known species, Candida rugopelliculosa, Pichia occidentalis, Pichia exigua and Candida phayaonensis, by 4.1-4.4% sequence divergence (22-24 substitutions and 0-2 gaps) in the D1/D2 sequences. Hyphopichia paragotoi sp. nov. belonged to the Hyphopichia clade, and its nearest phylogenetic neighbours were Candida gotoi, Candida pseudorhagii, Candida rhagii and Hyphopichia heimii with 3.2-4.2% sequence divergence (16-21 substitutions and 1 gap) in the D1/D2 sequences. In comparison with previously established species, H. paragotoi sp. nov. formed one hat-shaped ascospore in a persistent ascus. The type strain of P. dushanensis sp. nov. is NYNU 14658(T) ( = CICC 33049(T) = CBS 13912(T)), and the type strain of H. paragotoi sp. nov. is NYNU 14666(T) ( = CICC 33048(T) = CBS 13913(T)).
authors.affiliation: School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, PR China.
authors.forename: Yong-Cheng
authors.initials: YC
authors.lastname: Ren
authors.affiliation: School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, PR China.
authors.forename: Si-Tong
authors.initials: ST
authors.lastname: Liu
authors.affiliation: School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, PR China.
authors.forename: Feng-Li
authors.initials: FL
authors.lastname: Hui
mesh_terms: D000818:Animals; D002681:China; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D007313:Insecta / Q000382:microbiology*; D007814:Larva / Q000382:microbiology; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010802:Phylogeny; D010843:Pichia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012335:RNA, Ribosomal / Q000235:genetics; D004718:Saccharomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D014934:Wood / Q000382:microbiology*
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D012335:RNA, Ribosomal