title: [Epidermal growth factor-like domain 7 promotes endothelial cell migration and angiogenesis by activating ERK signaling pathway].
abstract: OBJECTIVE
  To explore the effect of epidermal growth factor-like domain 7(EGFL7) on the migration and angiogenesis of endothelial cells.
  
  
  METHODS
  EGFL7 overexpression vectors were constructed and transfected into human microvascular endothelial cells. The expression levels of EGFL7-mRNA and EGFL7 protein were examined by real-time RT-PCR and Western blot. Cell migration was analyzed by the wound healing. The capability of cell to form capillary-like tubes in vitro was evaluated on matrigel assay. Protein expression of p-AKT, AKT, p-ERK and ERK in endothelial cells was detected by Western blot upon transfection with EGFL7 overexpression vectors and vehicle control for 0, 10, 30 and 60 min.
  
  
  RESULTS
  Migration and angiogenesis of endothelial cells were notably enhanced by EGFL7 overexpression. ERK pathway was strongly activated by EGFL7, whereas AKT remained constant in endothelial cells. Inhibition of ERK impaired EGFL7 induced ERK activation and endothelial cell migration and angiogenesis.
  
  
  CONCLUSIONS
  EGFL7 effectively promotes migration and angiogenesis through ERK signaling pathway in endothelial cells.
authors.affiliation: Department of Traumatic Orthopedic, Henan Provincial Hospital of Traditional Chinese Medicine, Zhengzhou 450002, China.
authors.forename: Yujing
authors.initials: Y
authors.lastname: Cao
authors.affiliation: E-mail: changliuxian@gmail.com.
authors.forename: Qiuxia
authors.initials: Q
authors.lastname: Lyu
mesh_terms: D015153:Blotting, Western; D002135:Calcium-Binding Proteins; D002465:Cell Movement; D066255:EGF Family of Proteins; D042783:Endothelial Cells / Q000502:physiology*; D016228:Endothelial Growth Factors / Q000235:genetics / Q000502:physiology*; D006801:Humans; D020935:MAP Kinase Signaling System / Q000502:physiology*; D018919:Neovascularization, Physiologic; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D002135:Calcium-Binding Proteins; D066255:EGF Family of Proteins; C527877:EGFL7 protein, human; D016228:Endothelial Growth Factors; D012333:RNA, Messenger