title: Probing a cell-embedded megadalton protein complex by DNP-supported solid-state NMR.
abstract: Studying biomolecules at atomic resolution in their native environment is the ultimate aim of structural biology. We investigated the bacterial type IV secretion system core complex (T4SScc) by cellular dynamic nuclear polarization-based solid-state nuclear magnetic resonance spectroscopy to validate a structural model previously generated by combining in vitro and in silico data. Our results indicate that T4SScc is well folded in the cellular setting, revealing protein regions that had been elusive when studied in vitro.
authors.affiliation: NMR Spectroscopy, Utrecht University, Utrecht, the Netherlands.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Kaplan
authors.affiliation: NMR Spectroscopy, Utrecht University, Utrecht, the Netherlands.
authors.forename: Abhishek
authors.identifier: 0000000215326869
authors.initials: A
authors.lastname: Cukkemane
authors.affiliation: NMR Spectroscopy, Utrecht University, Utrecht, the Netherlands.
authors.forename: Gydo C P
authors.initials: GC
authors.lastname: van Zundert
authors.affiliation: NMR Spectroscopy, Utrecht University, Utrecht, the Netherlands.
authors.forename: Siddarth
authors.initials: S
authors.lastname: Narasimhan
authors.affiliation: NMR Spectroscopy, Utrecht University, Utrecht, the Netherlands.
authors.forename: Mark
authors.initials: M
authors.lastname: Daniëls
authors.affiliation: NMR Spectroscopy, Utrecht University, Utrecht, the Netherlands.
authors.forename: Deni
authors.initials: D
authors.lastname: Mance
authors.affiliation: Institute of Structural and Molecular Biology, University College London and Birkbeck, London, UK.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Waksman
authors.affiliation: NMR Spectroscopy, Utrecht University, Utrecht, the Netherlands.
authors.forename: Alexandre M J J
authors.initials: AM
authors.lastname: Bonvin
authors.affiliation: Groupe à 5 ans, Biologie structurale de la secretion bacterienne, Unité mixte de recherche Centre National de la Recherche Scientifique-Institut Pasteur 3528, Institut Pasteur, Paris, France.
authors.forename: Rémi
authors.initials: R
authors.lastname: Fronzes
authors.affiliation: NMR Spectroscopy, Utrecht University, Utrecht, the Netherlands.
authors.forename: Gert E
authors.initials: GE
authors.lastname: Folkers
authors.affiliation: NMR Spectroscopy, Utrecht University, Utrecht, the Netherlands.
authors.forename: Marc
authors.initials: M
authors.lastname: Baldus
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017510:Protein Folding
chemical_list: D001426:Bacterial Proteins