title: Ultrastructural calcium distribution and myocardial calcium content in human idiopathic dilated cardiomyopathy.
abstract: Myocardial calcium overload in chronic heart failure is still a debatable issue. The aim of this study was to investigate the myocardial calcium content and intracellular calcium distribution in end-stage dilated cardiomyopathy. The explanted hearts of 13 patients (9 male, 4 female, mean age 49 ± 12 years) undergoing heart transplantation because of end-stage dilated cardiomyopathy were examined. Samples were obtained from the right and left ventricular free wall and from the septum. Calcium and magnesium content were measured by atomic absorption spectrophotometry. Ultrastructural calcium distribution was examined in dilated cardiomyopathy using the phosphate-pyroantimonate method. Ultrastructural calcium distribution was also examined in left ventricular biopsies obtained from 3 patients (male, mean age 47 ± 3.6 years) with nonfailing hearts. The number of mitochondrial calcium precipitates was estimated morphometrically by a point counting method. Myocardial calcium and magnesium content in dilated cardiomyopathy did not differ significantly among the right and left ventricles and septum ranging from 8.5 to 10.8 mmol/kg dry weight. The phosphate-pyroantimonate method visualized calcium precipitates being confined to the sarcolemma, T-tubules, intercalated disks, and mitochondria in both nonfailing myocardium and dilated cardiomyopathy. Because mitochondria may act as buffers of cytoplasmic calcium, mitochondrial calcium precipitates served as a criterion for a possible cellular calcium overload. No differences in the amount of mitochondrial calcium deposits were observed between dilated cardiomyopathy and nonfailing hearts. The data suggest that there is no global myocardial calcium overload in human eng-stage dilated cardiomyopathy.
authors.affiliation: Zentrum der Inneren Medizin, Universität Frankfurt, Germany.
authors.forename: H G
authors.initials: HG
authors.lastname: Olbrich
authors.affiliation: Zentrum der Inneren Medizin, Universität Frankfurt, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Michaelis
authors.forename: G
authors.initials: G
authors.lastname: Vandeplassche
authors.affiliation: Zentrum der Chirurgie, Universität Frankfurt, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Borgers
authors.affiliation: Zentrum der Inneren Medizin, Universität Frankfurt, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Oremek
authors.affiliation: Janssen Research Foundation, Beerse, Belgium.
authors.forename: E
authors.initials: E
authors.lastname: Krause
authors.affiliation: Janssen Research Foundation, Beerse, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: Satter
authors.affiliation: Zentrum der Inneren Medizin, Universität Frankfurt, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Kober
authors.affiliation: Pharmakologisches Institut für Naturwissenschaftler, Universität Frankfurt, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Mutschler
authors.affiliation: Zentrum der Inneren Medizin, Universität Frankfurt, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Kaltenbach