title: Comparative study of the biochemical properties of proteasomes in domestic animals.
abstract: Information on the biochemical properties of proteasomes is lacking or, at best, only fragmentary for most species of veterinary interest. Moreover, direct comparison of the limited data available on the enzymatic features of proteasomes in domestic animals is rendered difficult due to the heterogeneity of the experimental settings used. This represents a clear drawback in veterinary research, given the crucial involvement of proteasomes in control of several physiological and pathological processes. We performed the first comparative analysis of key biochemical properties of proteasomes obtained from 8 different domestic mammals. Specifically, we investigated the three main peptidase activities of constitutive and immunoproteasomes in parallel and systematically checked the sensitivity of the chymotryptic site to three of the most potent and selective inhibitors available. Overall, there was substantial similarity in the enzymatic features of proteasomes among the species examined, although some interesting species-specific features were observed.
authors.affiliation: Department of Veterinary Sciences, University of Turin, Largo Braccini, 2, Grugliasco, Italy, 10095. Electronic address: mary.raule@unito.it.
authors.forename: Mary
authors.initials: M
authors.lastname: Raule
authors.affiliation: Department of Veterinary Sciences, University of Turin, Largo Braccini, 2, Grugliasco, Italy, 10095. Electronic address: fulvia.cerruti@unito.it.
authors.forename: Fulvia
authors.initials: F
authors.lastname: Cerruti
authors.affiliation: Department of Veterinary Sciences, University of Turin, Largo Braccini, 2, Grugliasco, Italy, 10095. Electronic address: paolo.cascio@unito.it.
authors.forename: Paolo
authors.initials: P
authors.lastname: Cascio
mesh_terms: D000818:Animals; D000829:Animals, Domestic / Q000378:metabolism*; D006801:Humans; D051379:Mice; D046988:Proteasome Endopeptidase Complex / Q000737:chemistry / Q000378:metabolism*; D021122:Protein Subunits; D013045:Species Specificity
chemical_list: D021122:Protein Subunits; D046988:Proteasome Endopeptidase Complex
keywords: Domestic animals; Immunoproteasome; Proteasome; Proteasome inhibitors; Proteasome peptidase activities; Proteolysis