title: Sevoflurane preconditioning improving cerebral focal ischemia-reperfusion damage in a rat model via PI3K/Akt signaling pathway.
abstract: In this study, we aimed to assess the neuroprotective effect of sevoflurane preconditioning in a cerebral focal ischemia-reperfusion rat model. Sixty Sprague Dawley rats were divided into six groups: sham operated group, cerebral focal ischemia-reperfusion (CIR) group, CIR+sevoflurane preconditioning (SP) (2%) group, CIR+sevoflurane preconditioning (2.5%) group, CIR+sevoflurane preconditioning (3%) group, and CIR+sevoflurane preconditioning (3.5%) group. All subjects were euthanized 2days post-surgery and their hippocampus tissues were removed. Tissue malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH) and glutathione peroxidase (GSH-Px) levels were measured and hippocampus tissue samples were examined histopathologically. Results showed that significant difference in antioxidant, immunity indexes, and apoptosis-related protein expression was detected in hippocampus tissue between sham-operated control and CIR groups. Sevoflurane preconditioning significantly dose-dependently reduced MDA, IL-1β, IL-6, IL-10 and TNF-α levels and enhanced antioxidant enzyme activities in hippocampus tissue of CIR+SP groups compared to CIR group. In addition, sevoflurane preconditioning significantly dose-dependently upregulated PI3K, p-Akt and Bcl-2 levels and downregulated caspase-3 and Bax levels in hippocampus tissue of CIR+SP groups compared to CIR group. It can be concluded that sevoflurane preconditioning demonstrates a strong and ameliorative effect on cerebral I/R damage in rats. The neuroprotective mechanisms of sevoflurane preconditioning are associated with its properties of anti-apoptosis and anti-oxidation as well as regulation of PI3K and p-Akt signal activation.
authors.affiliation: Department of Anesthesiology, Tianjin Huanhu Hospital, Tianjin Neurosurgery Institute, Tianjin 300060, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology, The First Hospital Affiliated Shanxi Medical University, Taiyuan, Shanxi 030001, China. Electronic address: shouyuantian5638@163.com.
authors.forename: Shou-Yuan
authors.initials: SY
authors.lastname: Tian
authors.affiliation: Department of Nephrology, The Second Hospital of Tianjin Medical University, Tianjin 300211, China.
authors.forename: Yan-Wei
authors.initials: YW
authors.lastname: Li
authors.affiliation: Department of Pharmacy, Tianjin Huanhu Hospital, Tianjin 300060, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology, Tianjin Nan Kai Hospital, Tianjin 300100, China. Electronic address: jianboyu5sk@163.com.
authors.forename: Jian-Bo
authors.initials: JB
authors.lastname: Yu
authors.affiliation: Department of Anesthesiology, Tianjin Medical University General Hospital, Tianjin 300052, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Tianjin Huanhu Hospital, Tianjin 300060, China.
authors.forename: Yi-Yang
authors.initials: YY
authors.lastname: Chen
authors.affiliation: Department of Anesthesiology, Tianjin Huanhu Hospital, Tianjin Neurosurgery Institute, Tianjin 300060, China.
authors.forename: Ya-Xin
authors.initials: YX
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, Tianjin Huanhu Hospital, Tianjin Neurosurgery Institute, Tianjin 300060, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Department of Anesthesiology, Tianjin Huanhu Hospital, Tianjin Neurosurgery Institute, Tianjin 300060, China.
authors.forename: Xiu-Shan
authors.initials: XS
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology, Tianjin Huanhu Hospital, Tianjin Neurosurgery Institute, Tianjin 300060, China.
authors.forename: Wen-Sheng
authors.initials: WS
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, Tianjin Huanhu Hospital, Tianjin Neurosurgery Institute, Tianjin 300060, China.
authors.forename: Hai-Gen
authors.initials: HG
authors.lastname: Liu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D002545:Brain Ischemia / Q000188:drug therapy* / Q000473:pathology; D005786:Gene Expression Regulation / Q000187:drug effects; D008738:Methyl Ethers / Q000008:administration & dosage*; D051058:Oncogene Protein v-akt / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051381:Rats; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000473:pathology; D000077149:Sevoflurane; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000975:Antioxidants; D008738:Methyl Ethers; D000077149:Sevoflurane; D019869:Phosphatidylinositol 3-Kinases; D051058:Oncogene Protein v-akt
keywords: Apoptosis; Cerebral focal ischemia–reperfusion; PI3K; Sevoflurane preconditioning