title: A novel NAP member GhNAP is involved in leaf senescence in Gossypium hirsutum.
abstract: Premature leaf senescence has a negative influence on the yield and quality of cotton, and several genes have been found to regulate leaf senescence. Howeer, many underlying transcription factors are yet to be identified. In this study, a NAP-like transcription factor (GhNAP) was isolated from Gossypium hirsutum. GhNAP has the typical NAC structure and a conserved novel subdomain in its divergent transcription activation region (TAR). GhNAP was demonstrated to be a nuclear protein, and it showed transcriptional activation activity in yeast. Furthermore, the expression of GhNAP was closely associated with leaf senescence. GhNAP could rescue the delayed-senescence phenotype of the atnap null mutant. Overexpression of GhNAP could cause precocious senescence in Arabidopsis. However, down-regulation of GhNAP delayed leaf senescence in cotton, and affected cotton yield and its fibre quality. Moreover, the expression of GhNAP can be induced by abscisic acid (ABA), and the delayed leaf senescence phenotype in GhNAPi plants might be caused by the decreased ABA level and reduced expression level of ABA-responsive genes. All of the results suggested that GhNAP could regulate the leaf senescence via the ABA-mediated pathways and was further related to the yield and quality in cotton.
authors.affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Kai
authors.initials: K
authors.lastname: Fan
authors.affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Noreen
authors.initials: N
authors.lastname: Bibi
authors.affiliation: Plant Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, NY 14853-5904, USA.
authors.forename: Susheng
authors.initials: S
authors.lastname: Gan
authors.affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Shuna
authors.initials: S
authors.lastname: Yuan
authors.affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Mi
authors.initials: M
authors.lastname: Ni
authors.affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Wang
authors.affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Shen
authors.affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, PR China xdwang@zju.edu.cn.
authors.forename: Xuede
authors.initials: X
authors.lastname: Wang
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D003368:Gossypium / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D009154:Mutation; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; C110811:NAP protein, Arabidopsis; D010940:Plant Proteins; D014157:Transcription Factors; D000040:Abscisic Acid
keywords: Abscisic acid; GhNAP; Gossypium hirsutum; NAP subfamily; leaf senescence; transcription factor.