title: Zoonotic Enterocytozoon bieneusi genotypes in Pere David's deer (Elaphurus davidianus) in Henan, China.
abstract: Enterocytozoon bieneusi is a zoonotic pathogen of the phylum Microspora that infects humans as well as a variety of animal species worldwide. While molecular epidemiologic studies have characterized this parasite in various hosts, isolates from many susceptible hosts have not yet been examined. In this study, E. bieneusi was isolated from 47 Pere David's deer (Elaphurus davidianus) in Henan, China and characterized via PCR analysis of the internal transcribed spacer (ITS) gene. E. bieneusi was detected in 16 out of 47 (34.0%) fecal specimens examined. Sequence analysis of the ITS revealed six known genotypes: type IV (4), EbpC (4), EbpA (4), BEB6 (2), COS-I (1), and COS-II (1). Of these, type IV, EbpC, and EbpA are known to cause human microsporidiosis worldwide, whereas the remaining genotypes are generally specific to ruminants. The present study indicated that Pere David's deer are naturally infected with E. bieneusi, predominantly with zoonotic genotypes, and may pose a risk for human transmission.
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Zhenjie
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Jianying
authors.initials: J
authors.lastname: Huang
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Md Robiul
authors.initials: MR
authors.lastname: Karim
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Zhao
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Haiju
authors.initials: H
authors.lastname: Dong
authors.affiliation: The Wild Animals Rescue Center of Henan Province, Zhengzhou 450044, China.
authors.forename: Weichang
authors.initials: W
authors.lastname: Ai
authors.affiliation: College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Fuhuang
authors.initials: F
authors.lastname: Li
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Longxian
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China. Electronic address: wrj-1978@163.com.
authors.forename: Rongjun
authors.initials: R
authors.lastname: Wang
mesh_terms: D000818:Animals; D002681:China / Q000453:epidemiology; D003670:Deer; D021862:Enterocytozoon / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology; D005838:Genotype; D016881:Microsporidiosis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D015047:Zoonoses
keywords: Enterocytozoon bieneusi; Genotyping; ITS; Pere David's deer