title: Pseudomonas coleopterorum sp. nov., a cellulase-producing bacterium isolated from the bark beetle Hylesinus fraxini.
abstract: We isolated a strain coded Esc2Am(T) during a study focused on the microbial diversity of adult specimens of the bark beetle Hylesinus fraxini. Its 16S rRNA gene sequence had 99.4% similarity with respect to its closest relative, Pseudomonas rhizosphaerae IH5(T). The analysis of partial sequences of the housekeeping genes rpoB, rpoD and gyrB confirmed that strain Esc2Am(T) formed a cluster with P. rhizosphaerae IH5(T) clearly separated from the remaining species of the genus Pseudomonas. Strain Esc2Am(T) had polar flagella and could grow at temperatures from 4 °C to 30 °C. The respiratory quinone was Q9 and the main fatty acids were C16 : 0, C18 : 1ω7c and/or C18 : 1ω6c in summed feature 8 and C16 : 1ω7c and/or C16 : 1ω6c in summed feature 3. DNA-DNA hybridization results showed 51% relatedness with respect to P. rhizosphaerae IH5(T). Oxidase, catalase and urease-positive, the arginine dihydrolase system was present but nitrate reduction and β-galactosidase production were negative. Aesculin hydrolysis was positive. Based on the results from the genotypic, phenotypic and chemotaxonomic analyses, we propose the classification of strain Esc2Am(T) as representing a novel species of the genus Pseudomonas, for which we propose the name Pseudomonas coleopterorum sp. nov. The type strain is Esc2Am(T) ( = LMG 28558(T)= CECT 8695(T)).
authors.affiliation: Microbiology and Genetics Department, University of Salamanca, Spain.
authors.forename: Esther
authors.initials: E
authors.lastname: Menéndez
authors.affiliation: Institute of Natural Resources and Agrobiology. IRNASA-CSIC, Salamanca, Spain.
authors.forename: Martha H
authors.initials: MH
authors.lastname: Ramírez-Bahena
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Anna
authors.initials: A
authors.lastname: Fabryová
authors.affiliation: Institute of Natural Resources and Agrobiology. IRNASA-CSIC, Salamanca, Spain.
authors.forename: José M
authors.initials: JM
authors.lastname: Igual
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Oldrich
authors.initials: O
authors.lastname: Benada
authors.affiliation: Microbiology and Genetics Department, University of Salamanca, Spain.
authors.forename: Pedro F
authors.initials: PF
authors.lastname: Mateos
authors.affiliation: Institute of Natural Resources and Agrobiology. IRNASA-CSIC, Salamanca, Spain.
authors.forename: Alvaro
authors.initials: A
authors.lastname: Peix
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Miroslav
authors.initials: M
authors.lastname: Kolařík
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Paula
authors.initials: P
authors.lastname: García-Fraile
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002480:Cellulase; D001517:Coleoptera / Q000382:microbiology*; D018153:Czech Republic; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011549:Pseudomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D002480:Cellulase