title: In vitro generation of functional dendritic cells differentiated from CD34 negative cells isolated from human umbilical cord blood.
abstract: Dendritic cells (DCs) are the most potent antigen-presenting cells that play a crucial role in the initiation of an immune response. As DC-based therapeutic applications is increasing, large-scale DC production is required for transplantation. Human umbilical cord blood (UCB) has been shown to contain a rare and precious population of hematopoietic stem cells (HSCs), which can give rise to DCs. The CD34 antigen has been widely used as a cell surface marker to identify HSCs. In this study, we used CD34 antibody to isolate CD34(+) and CD34(-) cells and compared the ability to differentiate into DCs. We used a two-step method combined with the magnetic bead sorting system to isolate CD34(+) and CD34(-) cells from human UCB. Analysis of cellular properties and functionality using a migration assay and T cell proliferation assay revealed no significant differences between CD34(+) cells and CD34(-) cells in their ability to generate DCs.
authors.affiliation: Department of Stem Cell Biology, School of Medicine, Konkuk University, Seoul, 143-701, Korea.|Center for Stem Cell Research, Institute of Advanced Biomedical Science, Konkuk University, Seoul, 143-701, Korea.
authors.forename: Yo Seph
authors.initials: YS
authors.lastname: Park
authors.affiliation: School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), UNIST-gil, 50, Ulsan 689-798, Korea.
authors.forename: Changsik
authors.initials: C
authors.lastname: Shin
authors.affiliation: Department of Obstetrics and Gynecology, Konkuk University School of Medicine, Seoul 143-729, Korea.
authors.forename: Han Sung
authors.initials: HS
authors.lastname: Hwang
authors.affiliation: Institute for Biomedical Engineering, Department of Cell Biology, RWTH Aachen University Medical School, Aachen University, Aachen, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Zenke
authors.affiliation: Department of Stem Cell Biology, School of Medicine, Konkuk University, Seoul, 143-701, Korea.
authors.forename: Dong Wook
authors.initials: DW
authors.lastname: Han
authors.affiliation: Department of Biomedical Science and Technology, Institute of Advanced Biomedical Science, Konkuk University, Seoul, 143-701, Korea.
authors.forename: Young Sun
authors.initials: YS
authors.lastname: Kang
authors.affiliation: Department of Medicine, College of Medicine, Chung-Ang University, Seoul, 156-756, Korea.
authors.forename: Kisung
authors.initials: K
authors.lastname: Ko
authors.affiliation: School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), UNIST-gil, 50, Ulsan 689-798, Korea.
authors.forename: Yoonkyung
authors.initials: Y
authors.lastname: Do
authors.affiliation: Department of Stem Cell Biology, School of Medicine, Konkuk University, Seoul, 143-701, Korea.|Center for Stem Cell Research, Institute of Advanced Biomedical Science, Konkuk University, Seoul, 143-701, Korea.|Research Institute of Medical Science, Konkuk University, Seoul, 143-701, Korea.
authors.forename: Kinarm
authors.initials: K
authors.lastname: Ko
mesh_terms: D018952:Antigens, CD34 / Q000276:immunology; D002454:Cell Differentiation / Q000276:immunology; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology; D005312:Fetal Blood / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D006801:Humans; D008213:Lymphocyte Activation
chemical_list: D018952:Antigens, CD34
keywords: CD34; dendritic cells; differentiation; hematopoietic stem cells; human umbilical cord blood