title: Lampropedia puyangensis sp. nov., isolated from symptomatic bark of Populus × euramericana canker and emended description of Lampropedia hyalina (Ehrenberg 1832) Lee et al. 2004.
abstract: A Gram-stain negative, Neisser-stain negative, aerobic, non-motile, non-spore-forming, slimy, glossy bacterial strain with single or clustered coccoid cells and white colony colour, designated as 2-bin(T), was isolated from cankered bark tissue of Populus × euramericana. The strain was found to grow at 15-40 °C and pH 5-10, with an optimum of 30 °C and pH 8.0. The strain was found to be negative with respect to catalase and positive for oxidase activity, nitrate reduction and Voges-Proskauer reaction. Analysis of 16S rRNA gene sequence data indicated that the isolate belongs to the genus Lampropedia, having sequence similarity of 96.24 % with Lampropedia hyalina ATCC11041(T). DNA-DNA relatedness of strain 2-bin(T) with L. hyalina JCM 21380(T) was 26.7 ± 4.6 %. The DNA G+C content of strain 2-bin(T) was determined to be 57 % and the major cellular fatty acids were identified as C16:0, C16:1 ω7c/C16:1 ω6c and C18:1 ω7c. The polar lipid profile of strain 2-bin(T) was found to contain diphosphatidylglycerol, a glycolipid, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, a phospholipid, phosphatidylmonomethylethanolamine and three unidentified lipids (L1, L2, L3). Based on molecular data and physiological and biochemical characteristics, strain 2-bin(T) is considered to represent a novel species in the genus Lampropedia, for which the name Lampropedia puyangensis sp. nov. is proposed. The type strain is 2-bin(T) (= CFCC 10925(T) = KCTC 32235(T)).
authors.affiliation: The Key Laboratory of State Forestry Administration on Forest Protection, Research Institute of Forest Ecology Environment and Protection, Chinese Academy of Forestry, Beijing, 100091, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Chun-Gen
authors.initials: CG
authors.lastname: Piao
authors.forename: Lai-Fa
authors.initials: LF
authors.lastname: Wang
authors.forename: Guo-Zhong
authors.initials: GZ
authors.lastname: Tian
authors.forename: Tian-Hui
authors.initials: TH
authors.lastname: Zhu
authors.forename: Min-Wei
authors.initials: MW
authors.lastname: Guo
mesh_terms: D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D042621:Comamonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006017:Glycolipids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D024301:Plant Bark / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology*; D032107:Populus / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D006017:Glycolipids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S