title: Cold adaptation of a psychrophilic chaperonin from Psychrobacter sp. and its application for heterologous protein expression.
abstract: OBJECTIVE
  A chaperonin, PsyGroELS, from the Antarctic psychrophilic bacterium Psychrobacter sp. PAMC21119, was examined for its role in cold adaptation when expressed in a mesophilic Escherichia coli strain.
  
  
  RESULTS
  Growth of E. coli harboring PsyGroELS at 10 °C was increased compared to the control strain. A co-expression system using PsyGroELS was developed to increase productivity of the psychrophilic enzyme PsyEst9. PsyEst9 was cloned and expressed using three E. coli variants that co-expressed GroELS from PAMC21119, E. coli, or Oleispira antarctica RB8(T). Co-expression with PsyGroELS was more effective for the production of PsyEst9 compared tothe other chaperonins.
  
  
  CONCLUSIONS
  PsyGroELS confers cold tolerance to E. coli, and shows potential as an effective co-expression system for the stable production of psychrophilic proteins.
authors.affiliation: Division of Polar Life Sciences, Korea Polar Research Institute (KOPRI), 26, Songdomirae-ro, Yeonsu-gu, Incheon, 406-840, Republic of Korea, hwkim@kopri.re.kr.
authors.forename: Han-Woo
authors.initials: HW
authors.lastname: Kim
authors.forename: Ah Ram
authors.initials: AR
authors.lastname: Wi
authors.forename: Byoung Wook
authors.initials: BW
authors.lastname: Jeon
authors.forename: Jun Hyuck
authors.initials: JH
authors.lastname: Lee
authors.forename: Seung Chul
authors.initials: SC
authors.lastname: Shin
authors.forename: Hyun
authors.initials: H
authors.lastname: Park
authors.forename: Sung-Jong
authors.initials: SJ
authors.lastname: Jeon
mesh_terms: D000222:Adaptation, Physiological; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D018833:Chaperonins / Q000235:genetics / Q000378:metabolism*; D003080:Cold Temperature; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D044096:Psychrobacter / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D018833:Chaperonins