title: [Flavonoids of Echinps latifolius suppress Wnt signaling in adjuvant arthritis rats].
abstract: The role of flavonoids of Echinps latifolius (FELT) in Wnt signaling was investigated in adjuvant arthritis (AA) rats. The therapeutic effects of FELT on AA rats were detected by rat arthritis score and MTT. The effect of FELT gavage treatment on the Wnt signaling key gene β-catenin, C-myc and cyclin D1 in synovium from AA rats was detected by Real-time qPCR, and the effects of FELT gavage treatment on the upstream negative regulation gene SFRP 1,2,4,5 in synovium from AA rats were detected by Real-time qPCR. The results showed that FELT gavage treatment significantly inhibited arthritis score and MTT values in AA rats, significantly inhibited the expression of the Wnt signaling gene β-catenin, C-myc and cyclin D1, significantly up-regulated the expression of the up- stream negative regulation gene SFRP 1,2,4. FELT has a better therapeutic effect for AA rats.
authors.forename: Cheng-Gui
authors.initials: CG
authors.lastname: Miao
authors.forename: Jian
authors.initials: J
authors.lastname: Xu
authors.forename: Hu
authors.initials: H
authors.lastname: Gao
authors.forename: Liang-Liang
authors.initials: LL
authors.lastname: Liu
authors.forename: Guo-Liang
authors.initials: GL
authors.lastname: Zhou
authors.forename: Mei-Song
authors.initials: MS
authors.lastname: Qin
authors.forename: Jian-Zhong
authors.initials: JZ
authors.lastname: Chen
authors.forename: Cheng-Feng
authors.initials: CF
authors.lastname: Li
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism*; D019659:Asteraceae / Q000737:chemistry*; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D005419:Flavonoids / Q000008:administration & dosage*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects*; D013583:Synovial Membrane / Q000187:drug effects / Q000378:metabolism; D060449:Wnt Signaling Pathway / Q000187:drug effects*; D051176:beta Catenin / Q000378:metabolism; D000094742:Secreted Frizzled-Related Proteins
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D036341:Intercellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D051176:beta Catenin; C500646:Sfrp1 protein, rat; D000094742:Secreted Frizzled-Related Proteins