title: Endomycorrhizal association ofFunneliformis mosseaewith transformed roots ofLinum usitatissimum: germination, colonization, and sporulation studies.
abstract: Spores of arbuscular mycorrhizal (AM) fungusFunneliformis mosseae(Nicolson & Gerdemann) Walker & Schuessler were cultured in association with transformed roots ofLinum usitatissimumL. (Linaceae) for the first time on modified Strullu-Romand medium (pH 5.5) in monoxenic culture. Germ tubes emerged through the spore wall in 88% of spores after 5 days. Hyphal contact with transformed linum roots was observed 5 days after co-cultivation.Paris-type arbuscules and hyphal coils were seen. Extra-radical branched absorbing structures were common. Terminal and intercalary secondary spores were also formed. Spore viability when assessed using vital dye staining (MTT test) was 83%. Secondary spores that proved viable were subsequently transferred fromin vitrotoin vivoculture whereArum-type arbuscules, intra-radical intercellular hyphae, and extra-radical spores were observed. The procedure established shows potential in AM inoculum mass production and possibility in application.
authors.affiliation: Department of Botany, Goa University , Goa , India.
authors.forename: Kim Maria
authors.initials: KM
authors.lastname: Rodrigues
authors.affiliation: Department of Botany, Goa University , Goa , India.
authors.forename: Bernard Felinov
authors.initials: BF
authors.lastname: Rodrigues
keywords: Funneliformis mosseae; MSR; in vivo; monoxenic culture; transformed linum roots