title: Method for sectioning and sampling hearts for histologic evaluation after delivery of biological agents by transendocardial injection.
abstract: The use of transendocardial (TE) injection as a validated method for delivering therapeutic agents to the diseased heart is increasing. Of the catheter systems currently available, TE injections guided by electromechanical mapping are attractive due to their minimal use of fluoroscopy and three-dimensional reconstruction capabilities that allow precise targeting of injections. We propose a method of cardiac sampling that takes advantage of the spatial accuracy of this system. Our preclinical experience with this methodology has yielded encouraging results, allowing a thorough examination of the injected areas through limited sampling.
authors.affiliation: Cardiovascular Pathology Research Department, Texas Heart Institute at CHI St. Luke's Health-Baylor St. Luke's, Houston, TX, USA. Electronic address: dvela@texasheart.org.
authors.forename: Deborah
authors.initials: D
authors.lastname: Vela
authors.affiliation: Stem Cell Center, Texas Heart Institute at CHI St. Luke's Health-Baylor St. Luke's, Houston, TX, USA.
authors.forename: Amir
authors.initials: A
authors.lastname: Gahremanpour
authors.affiliation: Cardiovascular Pathology Research Department, Texas Heart Institute at CHI St. Luke's Health-Baylor St. Luke's, Houston, TX, USA; The University of Texas Health Science Center at Houston, Houston, TX, USA.
authors.forename: L Maximilian
authors.initials: LM
authors.lastname: Buja
mesh_terms: D000818:Animals; D002309:Cardiology / Q000379:methods*; D002317:Cardiovascular Agents / Q000008:administration & dosage; D006321:Heart; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D007267:Injections / Q000379:methods
chemical_list: D002317:Cardiovascular Agents
keywords: Electromechanical mapping; Pathology; Transendocardial injections