title: miR-25 targets the modulator of apoptosis 1 gene in lung cancer.
abstract: To determine the role of miR-25 in non-small cell lung cancer (NSCLC), we first detected miR-25 expression in clinical specimens and lung cancer cell lines by quantitative real-time polymerase chain reaction. The levels of miR-25 were elevated in the plasma of NSCLC patients and NSCLC cell lines. Transfection of A549 and 95-D cells with a miR-25 inhibitor resulted in reduced cell proliferation and enhanced apoptosis. Moreover, the modulator of apoptosis 1 (MOAP1) gene was identified as a novel target of miR-25. The ability of miR-25 to promote cell proliferation and block apoptosis is attributable to its effect on MOAP1 suppression. In addition, miR-25 antagomir significantly inhibited lung cancer growth via upregulation of MOAP1 in a mouse xenograft model. Collectively, these data demonstrate that miR-25 is an important biomarker for lung cancer, and miR-25 promotes cell proliferation and inhibits apoptosis in NSCLC cells by negatively regulating MOAP1 expression.
authors.affiliation: Department of Medical Laboratory.
authors.forename: Tangwei
authors.initials: T
authors.lastname: Wu
authors.affiliation: Department of Medical Laboratory, Department of Central Laboratory, Cancer Research Institute of Wuhan and.
authors.forename: Weiqun
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Medical Laboratory.
authors.forename: Deyong
authors.initials: D
authors.lastname: Kong
authors.affiliation: Department of Medical Laboratory.
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Li
authors.affiliation: Cancer Research Institute of Wuhan and Department of Oncology, Central Hospital of Wuhan, 26 Shengli St., Jiangan District, Wuhan 430014, China.
authors.forename: Hongda
authors.initials: H
authors.lastname: Lu
authors.affiliation: Cancer Research Institute of Wuhan and.
authors.forename: Shuiyi
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Medical Laboratory.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Medical Laboratory.
authors.forename: Lili
authors.initials: L
authors.lastname: Du
authors.affiliation: Department of Central Laboratory, Cancer Research Institute of Wuhan and Department of Oncology, Central Hospital of Wuhan, 26 Shengli St., Jiangan District, Wuhan 430014, China.
authors.forename: Qingzhi
authors.initials: Q
authors.lastname: Kong
authors.affiliation: Department of Central Laboratory, Cancer Research Institute of Wuhan and.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Huang
authors.affiliation: Department of Medical Laboratory, Department of Central Laboratory, Cancer Research Institute of Wuhan and lzx71@yahoo.com.
authors.forename: Zhongxin
authors.initials: Z
authors.lastname: Lu
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics*; D000368:Aged; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D051017:Apoptosis Regulatory Proteins / Q000235:genetics*; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D023041:Xenograft Model Antitumor Assays
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; C543351:MIRN25 microRNA, human; C428449:MOAP1 protein, human; D035683:MicroRNAs