title: Creation and Analysis of a Virome: Using CRISPR Spacers.
abstract: Advances in sequencing technology have allowed for the study of complex and previously unexplored microbial and viral populations; however, linking host-phage partners using in silico techniques has been challenging. Here, we describe the flow-through for creation of a virome, and its subsequent analysis with the viral assembly and analysis module "Viritas," which we have recently developed. This module allows for binning of contigs based on tetranucleotide frequencies, putative phage-host partner identification by CRISPR spacer matching, and identification of ORFs.
authors.affiliation: Department of Plant Biology, Carnegie Institution for Science, Stanford University, 260 Panama Street, Stanford, CA, 94305, USA.
authors.forename: Michelle
authors.initials: M
authors.lastname: Davison
authors.forename: Devaki
authors.initials: D
authors.lastname: Bhaya
mesh_terms: D001435:Bacteriophages / Q000235:genetics* / Q000502:physiology; D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics*; D023281:Genomics