title: Developing a genetically encoded green fluorescent protein mutant for sensitive light-up fluorescent sensing and cellular imaging of Hg(II).
abstract: Hg(II) is well-known for quenching fluorescence in a distance dependent manner. Nevertheless, when we exposed the fluorophore of a green fluorescent protein (GFP) toward Hg(II), through H148C mutation, the GFP fluorescence could be "lighted up" by Hg(II) down to sub-nM level. The detection linear range is 0.5-3.0 nM for protein solutions at 8.0 nM. The GFPH148C protein displayed a promising selectivity toward Hg(II) and also the cellular imaging capacity. Spectra measurements suggested that the ground-state redistribution of protein contributed to the fluorescence enhancement, which was found not limited to Hg(II), and thus presented an opening for building a pool of GFP-based chemosensors toward other heavy metal ions.
authors.affiliation: Department of Chemistry, College of Chemistry and Chemical Engineering, the MOE Key Laboratory of Analytical Sciences, Xiamen University, Xiamen 361005, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.affiliation: Department of Chemistry, College of Chemistry and Chemical Engineering, the MOE Key Laboratory of Analytical Sciences, Xiamen University, Xiamen 361005, China.
authors.forename: Daiping
authors.initials: D
authors.lastname: Guo
authors.affiliation: Department of Chemistry, College of Chemistry and Chemical Engineering, the MOE Key Laboratory of Analytical Sciences, Xiamen University, Xiamen 361005, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Chemistry, College of Chemistry and Chemical Engineering, the MOE Key Laboratory of Analytical Sciences, Xiamen University, Xiamen 361005, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Chemistry, College of Chemistry and Chemical Engineering, the MOE Key Laboratory of Analytical Sciences, Xiamen University, Xiamen 361005, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Li
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Disease, School of Life Science, Xiamen University, Xiamen 361005, China.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Disease, School of Life Science, Xiamen University, Xiamen 361005, China.
authors.forename: Boyuan
authors.initials: B
authors.lastname: Yin
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Disease, School of Life Science, Xiamen University, Xiamen 361005, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Xia
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Disease, School of Life Science, Xiamen University, Xiamen 361005, China.
authors.forename: Jixian
authors.initials: J
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Disease, School of Life Science, Xiamen University, Xiamen 361005, China.
authors.forename: Wenxin
authors.initials: W
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Disease, School of Life Science, Xiamen University, Xiamen 361005, China.
authors.forename: Ningshao
authors.initials: N
authors.lastname: Xia
authors.affiliation: Department of Chemistry, College of Chemistry and Chemical Engineering, the MOE Key Laboratory of Analytical Sciences, Xiamen University, Xiamen 361005, China. Electronic address: ybjiang@xmu.edu.cn.
authors.forename: Yunbao
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D002413:Cations, Divalent / Q000032:analysis; D004926:Escherichia coli / Q000737:chemistry / Q000166:cytology* / Q000235:genetics; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000737:chemistry* / Q000235:genetics; D008628:Mercury / Q000032:analysis*; D008856:Microscopy, Fluorescence; D016297:Mutagenesis, Site-Directed; D061848:Optical Imaging; D017354:Point Mutation; D013050:Spectrometry, Fluorescence; D015854:Up-Regulation
chemical_list: D002413:Cations, Divalent; D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins; D008628:Mercury
keywords: Cellular imaging; Fluorescence light-up; Green fluorescent protein-based sensor; Mercury ion