title: Interleukin-1β, Interleukin1-Ra, Interleukin-10, and tumor necrosis factor-α polymorphisms in Tunisian patients with rheumatoid arthritis.
abstract: OBJECTIVE
  The aim of this study was to investigate the role of IL-1β (-511C>T), TNFα (-308 G>A), IL-10 (-1082 G>A) and IL-1RN VNTR polymorphisms in the susceptibility to rheumatoid arthritis (RA) in Tunisians.
  
  
  METHODS
  Using PCR-based methods, 104 RA patients and 150 healthy controls were investigated. We compared allele and genotype frequencies in RA patients versus controls and analyzed their correlations with erosive form (EF).
  
  
  RESULTS
  IL1-RN VNTR A1A3 genotype is associated with higher risk of RA (P=0.012, OR=4.31). Among the cases, males who carry this genotype were more exposed to RA (P=0.044, OR=8, 47). For IL1- β gene, a significantly higher frequency of the -511C/C genotype was observed in RA patients in comparison to controls (P=0.013, OR=2.45). This higher frequency was especially observed in women (P=0,003, OR=3.42). In contrast, IL10-1082G/G genotype was less common in patients (P=0.046, OR=0.46). According to EF, men carrying IL1-RN VNTR A1A3 (P=0.005 OR=5.28) and IL1-β-511C/C (P=0.015 OR=2.61) genotypes develop non EF of RA. Moreover, TNFα-308 A allele (P=0.024, OR=1.84) and A/A genotype (P=0.033, OR=3.1) were positively associated to EF of RA. However, G allele (P=0.024, OR=0.31) and GG genotype (P=0.31, OR=0.031) of the TNFα-308 were protectors.
  
  
  CONCLUSIONS
  Our results indicated that IL-1RN VNTR, IL-1β (-511C>T) and IL-10 (-1082 G>A) are associated with susceptibility to RA, and that IL-1RN VNTR, IL-1β (-511C>T) and TNFα (-308 G>A) are associated with severity of RA.
authors.affiliation: Laboratory of Micro-Organisms and Active Biomolecules, Faculty of Sciences of Tunis, El Manar University, 1092 Tunis, Tunisia; Laboratory of Immunology, Military Hospital of Tunis, Tunis, Tunisia.
authors.forename: A
authors.initials: A
authors.lastname: Lagha
authors.affiliation: Laboratory of Micro-Organisms and Active Biomolecules, Faculty of Sciences of Tunis, El Manar University, 1092 Tunis, Tunisia.
authors.forename: S
authors.initials: S
authors.lastname: Zidi
authors.affiliation: Research Unit of Hematological and Autoimmune Diseases, Faculty of Pharmacy, University of Monastir, Tunisia.
authors.forename: M
authors.initials: M
authors.lastname: Stayoussef
authors.affiliation: Laboratory of Immunology, Military Hospital of Tunis, Tunis, Tunisia.
authors.forename: E
authors.initials: E
authors.lastname: Gazouani
authors.affiliation: Laboratory of Immunology, Military Hospital of Tunis, Tunis, Tunisia.
authors.forename: R
authors.initials: R
authors.lastname: Kochkar
authors.affiliation: Habib Thameur Hospital. Tunis, Tunisia.
authors.forename: S
authors.initials: S
authors.lastname: Kochbati
authors.affiliation: Department of Medical Biochemistry, Arabian Gulf University, Manama, Bahrain.
authors.forename: W Y
authors.initials: WY
authors.lastname: Almawi
authors.affiliation: Laboratory of Micro-Organisms and Active Biomolecules, Faculty of Sciences of Tunis, El Manar University, 1092 Tunis, Tunisia. Electronic address: loueslatibesma@gmail.com.
authors.forename: B
authors.initials: B
authors.lastname: Yacoubi-Loueslati
mesh_terms: D000328:Adult; D000483:Alleles; D001172:Arthritis, Rheumatoid / Q000453:epidemiology / Q000208:ethnology / Q000235:genetics*; D005006:Ethnicity / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein / Q000235:genetics*; D016753:Interleukin-10 / Q000235:genetics*; D053583:Interleukin-1beta / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D018598:Minisatellite Repeats; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D011446:Prospective Studies; D012720:Severity of Illness Index; D017678:Sex Distribution; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D014416:Tunisia / Q000453:epidemiology
chemical_list: C508609:IL10 protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10
keywords: Arthrite rhumatoïde; Gene polymorphism; IL-10; IL-1β; IL1-Ra; Polymorphisme du gène; Rheumatoid arthritis; Susceptibility; Susceptibilité; TNF-α