title: Probing the binding interaction of human serum albumin with three bioactive constituents of Eriobotrta japonica leaves: Spectroscopic and molecular modeling approaches.
abstract: Corosolic acid (CRA), maslinic acid (MA), and tormentic acid (TA) are three kind of bioactive constituents of Eriobotrta japonica leaves. In this study, plasma protein binding model prediction suggested that the binding ability to HSA was CRA>MA>TA. Furthermore, fluorescence spectroscopy confirmed this prediction. The results from emission and time resolved fluorescence studies revealed that the emission quenching of HSA with CRA, MA, and TA were all initiated by static quenching mechanism. From molecular docking results and site marker competitive experimental results it was possible to make good estimates about CRA, MA, and TA mainly bound to subdomain IIA of HSA. 3D fluorescence, FT-IR and CD spectra indicated that the local conformation of HSA molecules was affected by the presence of CRA, MA, and TA, but at different extents.
authors.affiliation: College of Chemical Engineering, Sichuan University, Chengdu 610065, People's Republic of China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.affiliation: College of Chemical Engineering, Sichuan University, Chengdu 610065, People's Republic of China.
authors.forename: Qiaomei
authors.initials: Q
authors.lastname: Sun
authors.affiliation: College of Chemical Engineering, Sichuan University, Chengdu 610065, People's Republic of China.
authors.forename: Xiangling
authors.initials: X
authors.lastname: Ma
authors.affiliation: College of Chemical Engineering, Sichuan University, Chengdu 610065, People's Republic of China.
authors.forename: Zaisheng
authors.initials: Z
authors.lastname: Rao
authors.affiliation: College of Chemical Engineering, Sichuan University, Chengdu 610065, People's Republic of China. Electronic address: lihuilab@sina.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
mesh_terms: D001665:Binding Sites; D002942:Circular Dichroism; D031983:Eriobotrya / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D062105:Molecular Docking Simulation; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D017550:Spectroscopy, Fourier Transform Infrared; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D009828:Oleanolic Acid / Q000031:analogs & derivatives
chemical_list: D012709:Serum Albumin; D014315:Triterpenes; C007695:euscaphic acid; C113861:corosolic acid; C412811:maslinic acid; D009828:Oleanolic Acid
keywords: Corosolic acid; Human serum albumin; Maslinic acid; Molecular modeling; Tormentic acid