title: JAM-A promotes wound healing by enhancing both homing and secretory activities of mesenchymal stem cells.
abstract: The homing ability and secretory function of mesenchymal stem cells (MSCs) are key factors that influence cell involvement in wound repair. These factors are controlled by multilayer regulatory circuitry, including adhesion molecules, core transcription factors (TFs) and certain other regulators. However, the role of adhesion molecules in this regulatory circuitry and their underlying mechanism remain undefined. In the present paper, we demonstrate that an adhesion molecule, junction adhesion molecule A (JAM-A), may function as a key promoter molecule to regulate skin wound healing by MSCs. In in vivo experiments, we show that JAM-A up-regulation promoted both MSC homing to full-thickness skin wounds and wound healing-related cytokine secretion by MSCs. In vitro experiments also showed that JAM-A promoted MSC proliferation and migration by activating T-cell lymphoma invasion and metastasis 1 (Tiam1). We suggest that JAM-A up-regulation can increase the proliferation, cytokine secretion and wound-homing ability of MSCs, thus accelerating the repair rate of full-thickness skin defects. These results may provide insights into a novel and potentially effective approach to improve the efficacy of MSC treatment.
authors.affiliation: Department of Histology and Embryology, Second Military Medical University, Shanghai, China Burns Institute of People's Liberation Army, Changhai Hospital, Second Military Medical University, Shanghai, China.
authors.forename: Minjuan
authors.initials: M
authors.lastname: Wu
authors.affiliation: Burns Institute of People's Liberation Army, Changhai Hospital, Second Military Medical University, Shanghai, China.
authors.forename: Shizhao
authors.initials: S
authors.lastname: Ji
authors.affiliation: Burns Institute of People's Liberation Army, Changhai Hospital, Second Military Medical University, Shanghai, China.
authors.forename: Shichu
authors.initials: S
authors.lastname: Xiao
authors.affiliation: Burns Institute of People's Liberation Army, Changhai Hospital, Second Military Medical University, Shanghai, China.
authors.forename: Zhengdong
authors.initials: Z
authors.lastname: Kong
authors.affiliation: Burns Institute of People's Liberation Army, Changhai Hospital, Second Military Medical University, Shanghai, China.
authors.forename: He
authors.initials: H
authors.lastname: Fang
authors.affiliation: Burns Institute of People's Liberation Army, Changhai Hospital, Second Military Medical University, Shanghai, China.
authors.forename: Yunqing
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Histology and Embryology, Second Military Medical University, Shanghai, China.
authors.forename: Kaihong
authors.initials: K
authors.lastname: Ji
authors.affiliation: Burns Institute of People's Liberation Army, Changhai Hospital, Second Military Medical University, Shanghai, China.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Department of Histology and Embryology, Second Military Medical University, Shanghai, China xiazhaofansmmu@163.com houqiliusmmu02@163.com.
authors.forename: Houqi
authors.initials: H
authors.lastname: Liu
authors.affiliation: Burns Institute of People's Liberation Army, Changhai Hospital, Second Military Medical University, Shanghai, China xiazhaofansmmu@163.com houqiliusmmu02@163.com.
authors.forename: Zhaofan
authors.initials: Z
authors.lastname: Xia
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002454:Cell Differentiation; D002465:Cell Movement; D002633:Chemotaxis; D018920:Coculture Techniques; D004817:Epidermis / Q000378:metabolism; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011956:Receptors, Cell Surface / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism; D014945:Wound Healing
chemical_list: D015815:Cell Adhesion Molecules; C407243:F11R protein, human; C498686:F11r protein, mouse; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D011956:Receptors, Cell Surface; D014157:Transcription Factors
keywords: JAM-A; homing; mesenchymal stem cells; secretion; wound repair