title: Diagnostics of Tree Diseases Caused by Phytophthora austrocedri Species.
abstract: We present methods for the detection and quantification of four Phytophthora species which are pathogenic on trees; Phytophthora ramorum, Phytophthora kernoviae, Phytophthora lateralis, and Phytophthora austrocedri. Nucleic acid extraction methods are presented for phloem tissue from trees, soil, and pure cultures on agar plates. Real-time PCR methods are presented and include primer and probe sets for each species, general advice on real-time PCR setup and data analysis. A method for sequence-based identification, useful for pure cultures, is also included.
authors.affiliation: Science and Advice for Scottish Agriculture, Roddinglaw Road, Edinburgh, EH12 9FJ, UK, vince.mulholland@sasa.gsi.gov.uk.
authors.forename: Vincent
authors.initials: V
authors.lastname: Mulholland
authors.forename: Matthew
authors.initials: M
authors.lastname: Elliot
authors.forename: Sarah
authors.initials: S
authors.lastname: Green
mesh_terms: D004271:DNA, Fungal / Q000032:analysis* / Q000235:genetics; D052585:Phloem / Q000235:genetics* / Q000382:microbiology; D010838:Phytophthora / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D010935:Plant Diseases / Q000382:microbiology*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity; D014197:Trees / Q000235:genetics* / Q000382:microbiology
chemical_list: D004271:DNA, Fungal