title: Oxaliplatin vs. cisplatin: competition experiments on their binding to lysozyme.
abstract: The model protein hen egg white lysozyme was challenged with oxaliplatin and cisplatin. ESI mass spectrometry, surface plasmon resonance and thermal shift analyses demonstrate the formation of a bis-platinum adduct, though in very small amounts. Crystals of the bis-platinum adduct were obtained using two different preparations and the X-ray structures were solved at 1.85 Å and 1.95 Å resolution. Overall, the obtained data point out that, under the analyzed conditions, the two Pt drugs have similar affinities for the protein, but bind on its surface at two non-overlapping sites. In other words, these two drugs manifest a significantly different reactivity with this model protein and do not compete for the same protein binding sites.
authors.affiliation: Department of Pharmacy, University of Naples Federico II, via Montesano 12, 80120, Napoli, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Marasco
authors.forename: Luigi
authors.initials: L
authors.lastname: Messori
authors.forename: Tiziano
authors.initials: T
authors.lastname: Marzo
authors.forename: Antonello
authors.initials: A
authors.lastname: Merlino
mesh_terms: D000970:Antineoplastic Agents / Q000378:metabolism*; D001665:Binding Sites; D002942:Circular Dichroism; D002945:Cisplatin / Q000378:metabolism*; D003461:Crystallography; D009113:Muramidase / Q000378:metabolism*; D009944:Organoplatinum Compounds / Q000378:metabolism*; D000077150:Oxaliplatin; D011485:Protein Binding; D021241:Spectrometry, Mass, Electrospray Ionization; D020349:Surface Plasmon Resonance
chemical_list: D000970:Antineoplastic Agents; D009944:Organoplatinum Compounds; D000077150:Oxaliplatin; C421864:hen egg lysozyme; D009113:Muramidase; D002945:Cisplatin