title: Bioeffects of micron-size magnesium particles on inflammatory cells and bone turnover in vivo and in vitro.
abstract: Magnesium (Mg) is a promising biodegradable metal offering many potential advantages over current scaffold technologies. Many studies have reported on the corrosion characteristics the Mg and its bioeffects in vitro and in vivo, but there are few studies on the biological effects of the corrosive products of Mg - the micron-size Mg particles (MgMPs). In this study, the effects of size-selected commercial MgMPs on bone turnover and macrophages were investigated in vivo and in vitro. We found that MgMPs were susceptible to engulfment by macrophages, leading to cell lysis, likely resulting from H2 gas production. We also found that the inflammatory cytokines IL-1, IL-6, and TNF-α were induced more strongly by titanium particles (TiMPs) group than by either MgMPs or control. Examination of the expression of bone remodeling markers revealed that MgMPs are beneficial for bone regeneration. Micro-CT scanning indicated that, 30 days postimplantation, unlike TiMPs, MgMPs had no adverse effect on either bone quality or quantity. We have investigated the bioeffects of micron-size MgMPs in vivo and in vitro, and our results indicate that MgMPs may promote bone regeneration without inducing inflammation. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 923-931, 2016.
authors.affiliation: Center for Translational Medicine Research and Development, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, 518055, China.
authors.forename: Chengzhi
authors.initials: C
authors.lastname: Guo
authors.affiliation: Center for Translational Medicine Research and Development, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, 518055, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.affiliation: Center for Translational Medicine Research and Development, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, 518055, China.|Orthopedics Department, The second affiliated hospital of Soochow university, Suzhou, Jiangsu, 215000, China.
authors.forename: Chang
authors.initials: C
authors.lastname: She
authors.affiliation: Center for Translational Medicine Research and Development, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, 518055, China.|Hunan University of Chinese Medicine, Changsha, Hunan, 410000, China.
authors.forename: Xiangzhi
authors.initials: X
authors.lastname: Shao
authors.affiliation: Center for Translational Medicine Research and Development, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, 518055, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Teng
authors.affiliation: Orthopedics Department, Wuhan No.1 Hospital, Wuhan, Hubei, 430022, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Feng
authors.affiliation: Laboratory for Reproductive Health, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, 518055, China.
authors.forename: Jian V
authors.initials: JV
authors.lastname: Zhang
authors.affiliation: Center for Translational Medicine Research and Development, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, 518055, China.
authors.forename: Pei-Gen
authors.initials: PG
authors.lastname: Ren
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects*; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism*; D008274:Magnesium / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D015846:Monokines / Q000096:biosynthesis; D010316:Particle Size; D000067996:RAW 264.7 Cells; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology
chemical_list: D015846:Monokines; D014025:Titanium; D008274:Magnesium
keywords: bone remodeling; corrosion; inflammation; magnesium; particles