title: Cancer targeting propensity of folate conjugated surface engineered multi-walled carbon nanotubes.
abstract: Our main aim in the present investigation was to investigate the cancer targeting potential of docetaxel (DTX) loaded, folic acid (FA) terminated, poly (ethylene glycol) (PEG) conjugated, surface engineered multi walled carbon nanotubes (DTX/FA-PEG-MWCNTs) in tumor bearing Balb/c mice. The percent loading efficiency of DTX/FA-PEG-MWCNTs and DTX loaded MWCNTS (DTX/MWCNTs) was calculated to be 93.40±3.82% and 76.30±2.62%, respectively. Flow cytometry analysis suggested that the DTX/FA-PEG-MWCNTs arrested MCF-7 cells' cycle in the G2 phase and was more cytotoxic as compared to DTX/MWCNTs as well as free drug solution. The obtained pharmacokinetic parameters clearly describe the biocompatibility of engineered nanotubes to degree of functionalization and ability for prolonged residence inside the body. DTX/FA-PEG-MWCNTs was found to be significantly more efficient in tumor suppression as compared with plain MWCNTs (non-targeted) as well as drug solution owing to the enhanced drug release from endosomes after internalization. The DTX/FA-PEG-MWCNTs showed highly significant prolonged survival span (40 days) as compared to DTX/MWCNTs (24 days), free DTX (19 days) and control group (12 days). Overall, we can conclude that the DTX/FA-PEG-MWCNTs shows higher cancer targeting propensity vis a vis minimal side effects in tumor bearing Balb/c mice.
authors.affiliation: Pharmaceutics Research Laboratory, Department of Pharmaceutical Sciences, Dr. H. S. Gour University, Sagar 470 003, India; Pharmaceutical Nanotechnology Research Laboratory, ISF College of Pharmacy, Moga 142 001, India. Electronic address: neelesh81mph@gmail.com.
authors.forename: Neelesh Kumar
authors.initials: NK
authors.lastname: Mehra
authors.affiliation: Pharmaceutics Research Laboratory, Department of Pharmaceutical Sciences, Dr. H. S. Gour University, Sagar 470 003, India; Pharmaceutical Nanotechnology Research Laboratory, ISF College of Pharmacy, Moga 142 001, India. Electronic address: jnarendr@yahoo.co.in.
authors.forename: N K
authors.initials: NK
authors.lastname: Jain
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D005492:Folic Acid / Q000737:chemistry*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D018625:Microscopy, Atomic Force; D008854:Microscopy, Electron; D037742:Nanotubes, Carbon; D009369:Neoplasms / Q000473:pathology*; D013499:Surface Properties; D014961:X-Ray Diffraction
chemical_list: D037742:Nanotubes, Carbon; D005492:Folic Acid
keywords: Anticancer activity; Carbon nanotubes; Docetaxel; Kaplan–Meier survival; Pharmacokinetic; Tumor growth inhibition