title: Protein and RNA expression of acid-sensing ion channels 2 and 3 in myocardial ischemia rats induced by isoproterenol.
abstract: OBJECTIVE
  To investigate the impact of electro-acupuncture at the Neiguan (PC 6) acupoint on protein and RNA expression of acid-sensing ion channel 2 (ASIC2) and ASIC3 in myocardial ischemia rats.
  
  
  METHODS
  Fifty male Sprague-Dawley rats were used, weighing (230 ± 50) g. The rats were randomized into a normal group A, model group B, Neiguan (PC 6) group C, Lieque (LU 7) group D, and A-shi points group E. There were 10 rats in each group. Rats were continuously administered 85 mg/ kg intravenous isoproterenol daily to establish the model. Successfully modeled rats in groups C, D, and E were given electro-acupuncture treatment. Each group of rats was sacrificed with chloral hydrate (1 mL/100 g) intraperitoneal injection. The left ventricular myocardium was extracted and placed at -70 °C until use. Western blot analysis and real-time PCR were performed to assay protein and RNA expressions of ASIC2 and ASIC3, respectively. Fold changes in RNA expression were quantified with the 2^ (-ΔΔCt) method. Blood samples were drawn from the aorta abdominalis and tested for creatine kinase-MB (CK-MB) and lactate dehydrogenase (LDH) levels using enzyme-linked immunosorbent assay.
  
  
  RESULTS
  Myocardial ischemia rats given electro-acupuncture at the Neiguan (PC 6) acupoint had significantly lower protein and RNA expression of ASIC2 and ASIC3, and CK-MB and LDH levels, compared with model rats (P < 0.01).
  
  
  CONCLUSIONS
  Electro-acupuncture at the Neiguan (PC 6) acupoint can not only decrease the protein and RNA expression of ASIC2 and ASIC3, but also inhibit the opening of ASICs and reduce the cardiomyocyte damage in myocardial ischemia rats.
authors.forename: Wang
authors.initials: W
authors.lastname: Shudong
authors.forename: Zhang
authors.initials: Z
authors.lastname: Lide
authors.forename: Dong
authors.initials: D
authors.lastname: Baoqiang
authors.forename: Zhang
authors.initials: Z
authors.lastname: Wenshun
authors.forename: Ho Chin
authors.initials: HC
authors.lastname: Ee
authors.forename: Guo
authors.initials: G
authors.lastname: Na
authors.forename: Chen
authors.initials: C
authors.lastname: Yiguo
mesh_terms: D062565:Acid Sensing Ion Channels / Q000235:genetics* / Q000378:metabolism; D015669:Acupuncture Points; D000818:Animals; D015671:Electroacupuncture; D006801:Humans; D007545:Isoproterenol / Q000009:adverse effects; D008297:Male; D017202:Myocardial Ischemia / Q000139:chemically induced / Q000235:genetics* / Q000378:metabolism / Q000628:therapy*; D012313:RNA / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C568269:ASIC3 protein, rat; D062565:Acid Sensing Ion Channels; C568425:Asic2 protein, rat; D012313:RNA; D007545:Isoproterenol