title: GENERAL CONTROL NONREPRESSED PROTEIN5-Mediated Histone Acetylation of FERRIC REDUCTASE DEFECTIVE3 Contributes to Iron Homeostasis in Arabidopsis.
abstract: Iron homeostasis is essential for plant growth and development. Here, we report that a mutation in GENERAL CONTROL NONREPRESSED PROTEIN5 (GCN5) impaired iron translocation from the root to the shoot in Arabidopsis (Arabidopsis thaliana). Illumina high-throughput sequencing revealed 879 GCN5-regulated candidate genes potentially involved in iron homeostasis. Chromatin immunoprecipitation assays indicated that five genes (At3G08040, At2G01530, At2G39380, At2G47160, and At4G05200) are direct targets of GCN5 in iron homeostasis regulation. Notably, GCN5-mediated acetylation of histone 3 lysine 9 and histone 3 lysine 14 of FERRIC REDUCTASE DEFECTIVE3 (FRD3) determined the dynamic expression of FRD3. Consistent with the function of FRD3 as a citrate efflux protein, the iron retention defect in gcn5 was rescued and fertility was partly restored by overexpressing FRD3. Moreover, iron retention in gcn5 roots was significantly reduced by the exogenous application of citrate. Collectively, these data suggest that GCN5 plays a critical role in FRD3-mediated iron homeostasis. Our results provide novel insight into the chromatin-based regulation of iron homeostasis in Arabidopsis.
authors.affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement (J.Xi., T.W., Z.L., Y.Y., Z.H., H.P., M.X., Z.N.), and College of Resources and Environmental Sciences (J.Xu, F.Y.), China Agricultural University, Beijing 100193, China;National Plant Gene Research Centre, Beijing 100193, China (J.Xi., T.W., Z.L., Y.Y., Z.H., H.P., M.X., Z.N.); andInstitut de Biologie des Plantes, Unité Mixte de Recherche 8618, Université Paris Sud, 91405 Orsay, France (D.Z.).
authors.forename: Jiewen
authors.identifier: http://orcid.org/0000-0003-3503-0739
authors.initials: J
authors.lastname: Xing
authors.affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement (J.Xi., T.W., Z.L., Y.Y., Z.H., H.P., M.X., Z.N.), and College of Resources and Environmental Sciences (J.Xu, F.Y.), China Agricultural University, Beijing 100193, China;National Plant Gene Research Centre, Beijing 100193, China (J.Xi., T.W., Z.L., Y.Y., Z.H., H.P., M.X., Z.N.); andInstitut de Biologie des Plantes, Unité Mixte de Recherche 8618, Université Paris Sud, 91405 Orsay, France (D.Z.).
authors.forename: Tianya
authors.initials: T
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement (J.Xi., T.W., Z.L., Y.Y., Z.H., H.P., M.X., Z.N.), and College of Resources and Environmental Sciences (J.Xu, F.Y.), China Agricultural University, Beijing 100193, China;National Plant Gene Research Centre, Beijing 100193, China (J.Xi., T.W., Z.L., Y.Y., Z.H., H.P., M.X., Z.N.); andInstitut de Biologie des Plantes, Unité Mixte de Recherche 8618, Université Paris Sud, 91405 Orsay, France (D.Z.).
authors.forename: Zhenshan
authors.initials: Z
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement (J.Xi., T.W., Z.L., Y.Y., Z.H., H.P., M.X., Z.N.), and College of Resources and Environmental Sciences (J.Xu, F.Y.), China Agricultural University, Beijing 100193, China;National Plant Gene Research Centre, Beijing 100193, China (J.Xi., T.W., Z.L., Y.Y., Z.H., H.P., M.X., Z.N.); andInstitut de Biologie des Plantes, Unité Mixte de Recherche 8618, Université Paris Sud, 91405 Orsay, France (D.Z.).
authors.forename: Jianqin
authors.initials: J
authors.lastname: Xu
authors.affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement (J.Xi., T.W., Z.L., Y.Y., Z.H., H.P., M.X., Z.N.), and College of Resources and Environmental Sciences (J.Xu, F.Y.), China Agricultural University, Beijing 100193, China;National Plant Gene Research Centre, Beijing 100193, China (J.Xi., T.W., Z.L., Y.Y., Z.H., H.P., M.X., Z.N.); andInstitut de Biologie des Plantes, Unité Mixte de Recherche 8618, Université Paris Sud, 91405 Orsay, France (D.Z.).
authors.forename: Yingyin
authors.initials: Y
authors.lastname: Yao
authors.affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement (J.Xi., T.W., Z.L., Y.Y., Z.H., H.P., M.X., Z.N.), and College of Resources and Environmental Sciences (J.Xu, F.Y.), China Agricultural University, Beijing 100193, China;National Plant Gene Research Centre, Beijing 100193, China (J.Xi., T.W., Z.L., Y.Y., Z.H., H.P., M.X., Z.N.); andInstitut de Biologie des Plantes, Unité Mixte de Recherche 8618, Université Paris Sud, 91405 Orsay, France (D.Z.).
authors.forename: Zhaorong
authors.identifier: http://orcid.org/0000-0002-1815-648X
authors.initials: Z
authors.lastname: Hu
authors.affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement (J.Xi., T.W., Z.L., Y.Y., Z.H., H.P., M.X., Z.N.), and College of Resources and Environmental Sciences (J.Xu, F.Y.), China Agricultural University, Beijing 100193, China;National Plant Gene Research Centre, Beijing 100193, China (J.Xi., T.W., Z.L., Y.Y., Z.H., H.P., M.X., Z.N.); andInstitut de Biologie des Plantes, Unité Mixte de Recherche 8618, Université Paris Sud, 91405 Orsay, France (D.Z.).
authors.forename: Huiru
authors.initials: H
authors.lastname: Peng
authors.affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement (J.Xi., T.W., Z.L., Y.Y., Z.H., H.P., M.X., Z.N.), and College of Resources and Environmental Sciences (J.Xu, F.Y.), China Agricultural University, Beijing 100193, China;National Plant Gene Research Centre, Beijing 100193, China (J.Xi., T.W., Z.L., Y.Y., Z.H., H.P., M.X., Z.N.); andInstitut de Biologie des Plantes, Unité Mixte de Recherche 8618, Université Paris Sud, 91405 Orsay, France (D.Z.).
authors.forename: Mingming
authors.initials: M
authors.lastname: Xin
authors.affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement (J.Xi., T.W., Z.L., Y.Y., Z.H., H.P., M.X., Z.N.), and College of Resources and Environmental Sciences (J.Xu, F.Y.), China Agricultural University, Beijing 100193, China;National Plant Gene Research Centre, Beijing 100193, China (J.Xi., T.W., Z.L., Y.Y., Z.H., H.P., M.X., Z.N.); andInstitut de Biologie des Plantes, Unité Mixte de Recherche 8618, Université Paris Sud, 91405 Orsay, France (D.Z.).
authors.forename: Futong
authors.identifier: http://orcid.org/0000-0001-8672-3610
authors.initials: F
authors.lastname: Yu
authors.affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement (J.Xi., T.W., Z.L., Y.Y., Z.H., H.P., M.X., Z.N.), and College of Resources and Environmental Sciences (J.Xu, F.Y.), China Agricultural University, Beijing 100193, China;National Plant Gene Research Centre, Beijing 100193, China (J.Xi., T.W., Z.L., Y.Y., Z.H., H.P., M.X., Z.N.); andInstitut de Biologie des Plantes, Unité Mixte de Recherche 8618, Université Paris Sud, 91405 Orsay, France (D.Z.).
authors.forename: Daoxiu
authors.identifier: http://orcid.org/0000-0002-1540-0598
authors.initials: D
authors.lastname: Zhou
authors.affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement (J.Xi., T.W., Z.L., Y.Y., Z.H., H.P., M.X., Z.N.), and College of Resources and Environmental Sciences (J.Xu, F.Y.), China Agricultural University, Beijing 100193, China;National Plant Gene Research Centre, Beijing 100193, China (J.Xi., T.W., Z.L., Y.Y., Z.H., H.P., M.X., Z.N.); andInstitut de Biologie des Plantes, Unité Mixte de Recherche 8618, Université Paris Sud, 91405 Orsay, France (D.Z.) nizf@cau.edu.cn.
authors.forename: Zhongfu
authors.initials: Z
authors.lastname: Ni
mesh_terms: D000107:Acetylation; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D018506:Gene Expression Regulation, Plant; D059014:High-Throughput Nucleotide Sequencing; D051548:Histone Acetyltransferases / Q000235:genetics* / Q000378:metabolism; D006657:Histones / Q000235:genetics* / Q000378:metabolism; D006706:Homeostasis; D007501:Iron / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D018517:Plant Roots / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D017422:Sequence Analysis, DNA; D000099224:p300-CBP-Associated Factor
chemical_list: D029681:Arabidopsis Proteins; D051548:Histone Acetyltransferases; D006657:Histones; D007501:Iron; D026901:Membrane Transport Proteins; C469252:FRD3 protein, Arabidopsis; C474636:GCN5 protein, Arabidopsis; D000099224:p300-CBP-Associated Factor