title: DIANA-miRPath v3.0: deciphering microRNA function with experimental support.
abstract: The functional characterization of miRNAs is still an open challenge. Here, we present DIANA-miRPath v3.0 (http://www.microrna.gr/miRPathv3) an online software suite dedicated to the assessment of miRNA regulatory roles and the identification of controlled pathways. The new miRPath web server renders possible the functional annotation of one or more miRNAs using standard (hypergeometric distributions), unbiased empirical distributions and/or meta-analysis statistics. DIANA-miRPath v3.0 database and functionality have been significantly extended to support all analyses for KEGG molecular pathways, as well as multiple slices of Gene Ontology (GO) in seven species (Homo sapiens, Mus musculus, Rattus norvegicus, Drosophila melanogaster, Caenorhabditis elegans, Gallus gallus and Danio rerio). Importantly, more than 600 000 experimentally supported miRNA targets from DIANA-TarBase v7.0 have been incorporated into the new schema. Users of DIANA-miRPath v3.0 can harness this wealth of information and substitute or combine the available in silico predicted targets from DIANA-microT-CDS and/or TargetScan v6.2 with high quality experimentally supported interactions. A unique feature of DIANA-miRPath v3.0 is its redesigned Reverse Search module, which enables users to identify and visualize miRNAs significantly controlling selected pathways or belonging to specific GO categories based on in silico or experimental data. DIANA-miRPath v3.0 is freely available to all users without any login requirement.
authors.affiliation: DIANA-Lab, Department of Electrical & Computer Engineering, University of Thessaly, 382 21 Volos, Greece Laboratory for Experimental Surgery and Surgical Research 'N.S. Christeas', Medical School of Athens, University of Athens, 11527 Athens, Greece ivlachos@lessr.eu.
authors.forename: Ioannis S
authors.initials: IS
authors.lastname: Vlachos
authors.affiliation: 'Athena' Research and Innovation Center, 11524 Athens, Greece University of Peloponnisos, Department of Informatics and Telecommunications, 22100 Tripoli, Greece.
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Zagganas
authors.affiliation: DIANA-Lab, Department of Electrical & Computer Engineering, University of Thessaly, 382 21 Volos, Greece.
authors.forename: Maria D
authors.initials: MD
authors.lastname: Paraskevopoulou
authors.affiliation: DIANA-Lab, Department of Electrical & Computer Engineering, University of Thessaly, 382 21 Volos, Greece.
authors.forename: Georgios
authors.initials: G
authors.lastname: Georgakilas
authors.affiliation: DIANA-Lab, Department of Electrical & Computer Engineering, University of Thessaly, 382 21 Volos, Greece.
authors.forename: Dimitra
authors.initials: D
authors.lastname: Karagkouni
authors.affiliation: 'Athena' Research and Innovation Center, 11524 Athens, Greece.
authors.forename: Thanasis
authors.initials: T
authors.lastname: Vergoulis
authors.affiliation: 'Athena' Research and Innovation Center, 11524 Athens, Greece ivlachos@lessr.eu.
authors.forename: Theodore
authors.initials: T
authors.lastname: Dalamagas
authors.affiliation: DIANA-Lab, Department of Electrical & Computer Engineering, University of Thessaly, 382 21 Volos, Greece 'Athena' Research and Innovation Center, 11524 Athens, Greece ivlachos@lessr.eu.
authors.forename: Artemis G
authors.initials: AG
authors.lastname: Hatzigeorgiou
mesh_terms: D000465:Algorithms; D000818:Animals; D003198:Computer Simulation; D006801:Humans; D020407:Internet; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D058977:Molecular Sequence Annotation; D051381:Rats; D012984:Software
chemical_list: D035683:MicroRNAs