title: Dietary Sugars Analysis: Quantification of Fructooligossacharides during Fermentation by HPLC-RI Method.
abstract: In this work, a simple chromatographic method is proposed and in-house validated for the quantification of total and individual fructooligossacharides (e.g., 1-kestose, nystose, and 1(F)-fructofuranosylnystose). It was shown that a high-performance liquid chromatography with refractive index detector could be used to monitor the dynamic of fructooligossacharides production via sucrose fermentation using Aspergillus aculeatus. This analytical technique may be easily implemented at laboratorial or industrial scale for fructooligossacharides mass-production monitoring allowing also controlling the main substrate (sucrose) and the secondary by-products (glucose and fructose). The proposed chromatographic method had a satisfactory intra- and inter-day variability (in general, with a relative standard deviation lower than 5%), high sensitivity for each sugar (usually, with a relative error lower than 5%), and low detection (lower than 0.06 ± 0.04 g/L) and quantification (lower than 0.2 ± 0.1 g/L) limits. The correct quantification of fructooligossacharides in fermentative media may allow a more precise nutritional formulation of new functional foods, since it is reported that different fructooligossacharides exhibit different biological activities and effects.
authors.affiliation: Centre of Biological Engineering (CEB), University of Minho , Braga , Portugal.
authors.forename: Daniela M
authors.initials: DM
authors.lastname: Correia
authors.affiliation: Centro de Investigação de Montanha (CIMO), Escola Superior Agrária, Instituto Politécnico de Bragança , Bragança , Portugal.
authors.forename: Luís G
authors.initials: LG
authors.lastname: Dias
authors.affiliation: Centre of Biological Engineering (CEB), University of Minho , Braga , Portugal ; Departamento de Engenharia Química e Biológica (DEQB), Instituto Superior de Engenharia de Coimbra, Instituto Politécnico de Coimbra , Coimbra , Portugal.
authors.forename: Ana C A
authors.initials: AC
authors.lastname: Veloso
authors.affiliation: Centro de Investigação de Montanha (CIMO), Escola Superior Agrária, Instituto Politécnico de Bragança , Bragança , Portugal.
authors.forename: Teresa
authors.initials: T
authors.lastname: Dias
authors.affiliation: Centre of Biological Engineering (CEB), University of Minho , Braga , Portugal.
authors.forename: Isabel
authors.initials: I
authors.lastname: Rocha
authors.affiliation: Centre of Biological Engineering (CEB), University of Minho , Braga , Portugal.
authors.forename: Lígia R
authors.initials: LR
authors.lastname: Rodrigues
authors.affiliation: Laboratory of Separation and Reaction Engineering (LSRE) - Associate Laboratory LSRE/LCM, Escola Superior Agrária, Instituto Politécnico de Bragança , Bragança , Portugal.
authors.forename: António M
authors.initials: AM
authors.lastname: Peres
keywords: HPLC-RI method; dietary sugars; fructooligossacharides; in-house validation; yield fermentation process