title: Effects of CaCl2 hydrothermal treatment of titanium implant surfaces on early epithelial sealing.
abstract: Improvement of oral epithelial adhesion to titanium (Ti) may significantly enhance the efficacy of dental implants. We aimed to investigate whether calcium chloride (CaCl2) hydrothermally treated (HT) Ti could promote sealing of the peri-implant epithelium (PIE) around the implant. Right maxillary first molars were extracted from rats and replaced with either CaCl2-HT implants (Ca-HT group), distilled water-HT implants (DW-HT group), or untreated implants (Cont group). After 4 weeks, the implant-PIE interface of the Ca-HT group exhibited a band of immunoreactive laminin-332, similar to the tooth-junctional epithelium interface, which was absent in the Cont and DW-HT groups at the upper portion. We also investigated the effect of Ca-HT on the attachment of rat oral epithelial cells (OECs). OEC adherence onto Ca-HT Ti plates was stronger with higher expression levels of adhesion proteins compared with Cont and DW-HT groups. These results indicate that HT with CaCl2 improves the integration of soft tissue cells with the Ti implant at 4 weeks after implantation, which might facilitate the development of a soft tissue barrier around the implant.
authors.affiliation: Section of Implant and Rehabilitative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan.
authors.forename: Wakana
authors.initials: W
authors.lastname: Oshiro
authors.affiliation: Section of Implant and Rehabilitative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan. Electronic address: ayukawa@dent.kyushu-u.ac.jp.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Ayukawa
authors.affiliation: Section of Implant and Rehabilitative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan.
authors.forename: Ikiru
authors.initials: I
authors.lastname: Atsuta
authors.affiliation: Section of Implant and Rehabilitative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Furuhashi
authors.affiliation: Section of Implant and Rehabilitative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan.
authors.forename: Jyunichi
authors.initials: J
authors.lastname: Yamazoe
authors.affiliation: Section of Implant and Rehabilitative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Kondo
authors.affiliation: Section of Implant and Rehabilitative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan.
authors.forename: Mami
authors.initials: M
authors.lastname: Sakaguchi
authors.affiliation: Section of Implant and Rehabilitative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Matsuura
authors.affiliation: Section of Implant and Rehabilitative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Tsukiyama
authors.affiliation: Section of Implant and Rehabilitative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Koyano
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002122:Calcium Chloride / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002478:Cells, Cultured; D015921:Dental Implants; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D007150:Immunohistochemistry; D020007:Implants, Experimental; D039663:Integrin beta4 / Q000378:metabolism; D008297:Male; D051190:Plectin / Q000378:metabolism; D017208:Rats, Wistar; D013499:Surface Properties / Q000187:drug effects; D013997:Time Factors; D014025:Titanium / Q000737:chemistry*; D014945:Wound Healing / Q000187:drug effects; D000097794:Kalinin
chemical_list: D002122:Calcium Chloride; D015815:Cell Adhesion Molecules; D015921:Dental Implants; D039663:Integrin beta4; D051190:Plectin; D014025:Titanium; D000097794:Kalinin
keywords: Adhesion structure; Calcium hydrothermal treatment; Dental implant; Epithelial cell; Titanium