title: Real-Time LAMP for Chalara fraxinea Diagnosis.
abstract: Chalara fraxinea is the causal agent of ash dieback, a disease affecting Fraxinus excelsior and F. angustifolia across Europe. Loop-mediated isothermal amplification (LAMP) is a rapid, DNA-based method which can be used for specific detection of plant pathogens in infected material. The combination of a rapid LAMP assay for C. fraxinea with a simple sample preparation method in a user-friendly kit format raises the potential for testing to be carried out away from conventional laboratory facilities, to expedite measure to manage this damaging disease.
authors.affiliation: The Food and Environment Research Agency, Sand Hutton, York, YO41 1LZ, UK, jenny.tomlinson@fera.gsi.gov.uk.
authors.forename: Jenny
authors.initials: J
authors.lastname: Tomlinson
authors.forename: Neil
authors.initials: N
authors.lastname: Boonham
mesh_terms: D001203:Ascomycota / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D004271:DNA, Fungal / Q000032:analysis* / Q000235:genetics; D031661:Fraxinus / Q000235:genetics* / Q000382:microbiology; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D010935:Plant Diseases / Q000382:microbiology*; D013045:Species Specificity
chemical_list: D004271:DNA, Fungal