title: Evaluation of arboviruses of public health interest in free-living non-human primates (Alouatta spp., Callithrix spp., Sapajus spp.) in Brazil.
abstract: BACKGROUND
  The aim of the present study was to evaluate the presence of arboviruses from the Flavivirus genus in asymptomatic free-living non-human primates (NHPs) living in close contact with humans and vectors in the States of Paraná and Mato Grosso do Sul, Brazil.
  
  
  METHODS
  NHP sera samples (total n = 80, Alouatta spp. n = 07, Callithrix spp. n = 29 and Sapajus spp. n = 44) were screened for the presence of viral genomes using reverse transcription polymerase chain reaction and 10% polyacrylamide gel electrophoresis techniques.
  
  
  RESULTS
  All of the samples were negative for the Flavivirus genome following the 10% polyacrylamide gel electrophoresis analysis.
  
  
  CONCLUSIONS
  These negative results indicate that the analyzed animals were not infected with arboviruses from the Flavivirus genus and did not represent a risk for viral transmission through vectors during the period in which the samples were collected.
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Paraná, Curitiba, Paraná, Brazil.
authors.forename: Tatiana Carneiro da
authors.initials: TC
authors.lastname: Rocha
authors.affiliation: Coordenadoria Estadual de Vigilância Epidemiológica, Secretaria Estadual do Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil.
authors.forename: Paulo Mira
authors.initials: PM
authors.lastname: Batista
authors.affiliation: Embrapa Gado de Corte, Embrapa, Campo Grande, Mato Grosso do Sul, Brazil.
authors.forename: Renato
authors.initials: R
authors.lastname: Andreotti
authors.affiliation: Departamento de Zoologia, Setor de Ciências Biológicas, Universidade Federal do Paraná, Curitiba, Paraná, Brazil.
authors.forename: Ana Caroline Dalla
authors.initials: AC
authors.lastname: Bona
authors.affiliation: Departamento de Zoologia, Setor de Ciências Biológicas, Universidade Federal do Paraná, Curitiba, Paraná, Brazil.
authors.forename: Mário Antônio Navarro da
authors.initials: MA
authors.lastname: Silva
authors.affiliation: Departamento de Medicina Veterinária, Setor de Ciências Agrárias, Universidade Federal do Paraná, Curitiba, Paraná, Brazil.
authors.forename: Rogério
authors.initials: R
authors.lastname: Lange
authors.affiliation: Instituto Latino-Americano de Ciências da Vida e da Natureza, Universidade Federal da Integração Latino-Americana, Foz do Iguaçu, Paraná, Brazil.
authors.forename: Walfrido Kühl
authors.initials: WK
authors.lastname: Svoboda
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Paraná, Curitiba, Paraná, Brazil.
authors.forename: Eliane Carneiro
authors.initials: EC
authors.lastname: Gomes
mesh_terms: D000508:Alouatta / Q000821:virology*; D000818:Animals; D000835:Animals, Wild; D001103:Arboviruses / Q000235:genetics / Q000302:isolation & purification*; D001938:Brazil; D002144:Callithrix / Q000821:virology*; D002353:Carrier State / Q000662:veterinary / Q000821:virology; D002428:Cebus / Q000821:virology*; D004591:Electrophoresis, Polyacrylamide Gel; D008992:Monkey Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012367:RNA, Viral