title: Sensitive determination of four camptothecins by solid-phase microextraction-HPLC based on a boronic acid contained polymer monolithic layer.
abstract: Camptothecin (CPT) and its derivative have been revealed to possess special anti-cancer activity, extraction methods are necessary for trace determination of CPTs in complex samples. In this work, we prepared a high efficient boronic acid-based polymer monolithic layer for microextraction of CPTs. A disposable membrane filter-based extraction device was developed, and boronic acid groups were co-polymerized into a polyporous polymer skeleton and served as the monolithic sorbent. The prepared poly(4-VB-MA-TRIM) showed good stability and great extraction efficiency toward four CPTs. After optimization of extraction conditions, poly(4-VB-MA-TRIM)-based solid-phase microextraction was coupled HPLC for determination of CPTs in biological samples. The method exhibited low limits of detection of 0.05-0.2 ng mL(-1), which is significantly more sensitive than reported HPLC methods. The method also showed wide linear range (0.1-100 and 0.5-200 ng mL(-1)), good linearity (R(2)≥0.9981) and good reproducibility (RSD ≤3.76%). The method has been applied in plasma samples, with good selectivity and good recoveries ranging from 85.1 to 104.7%.
authors.affiliation: Affiliated Dongfeng Hospital, Hubei University of Medicine, Hubei 442008, China.
authors.forename: Jishun
authors.initials: J
authors.lastname: Chen
authors.affiliation: Affiliated Dongfeng Hospital, Hubei University of Medicine, Hubei 442008, China.
authors.forename: Xinwen
authors.initials: X
authors.lastname: Min
authors.affiliation: Affiliated Dongfeng Hospital, Hubei University of Medicine, Hubei 442008, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.affiliation: Affiliated Dongfeng Hospital, Hubei University of Medicine, Hubei 442008, China.
authors.forename: Wu
authors.initials: W
authors.lastname: Chen
authors.affiliation: Affiliated Dongfeng Hospital, Hubei University of Medicine, Hubei 442008, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Tian
authors.affiliation: Affiliated Dongfeng Hospital, Hubei University of Medicine, Hubei 442008, China. Electronic address: cqh77@163.com.
authors.forename: Qinhua
authors.initials: Q
authors.lastname: Chen
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000097:blood* / Q000302:isolation & purification; D001897:Boronic Acids / Q000737:chemistry*; D002166:Camptothecin / Q000097:blood* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D006801:Humans; D057230:Limit of Detection; D011108:Polymers / Q000737:chemistry*; D052617:Solid Phase Microextraction / Q000295:instrumentation / Q000379:methods*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D001897:Boronic Acids; D011108:Polymers; D002166:Camptothecin
keywords: Boronic acid; Camptothecin; HPLC; Plasma; Solid-phase microextraction