title: Comparative pharmacokinetic profiles of tectorigenin in rat plasma by UPLC-MS/MS after oral administration of Iris tectorum Maxim extract and pure tectoridin.
abstract: Iris tectorum Maxim, a well-known herb medicine, is commonly used for treatment of inflammation, cough, and pharyngitis for a long time in China. Tectoridin, main active ingredient of Iris tectorum Maxim, is often used for its quality control. This study was aimed to analyze the pharmacokinetic profile of tectorigenin (the metabolite of tectoridin) after oral administration of I. tectorum Maxim extract, and to compare the pharmacokinetic characterization of tectorigenin after oral administration of I. tectorum Maxim extract (ITME) and pure tectoridin (PT) in rats. In addition, a simple, reliable and sensitive UPLC-MS/MS method was developed for determination of tectorigenin in rat plasma, using kaempferol as internal standard. The processed samples were separated on a Poroshell 120 SB-C₁₈ column and detected by positive electrospray ionization in multiple reaction monitoring (MRM) mode. The method validation results indicated that the established method was simple, specific and reliable. The pharmacokinetic results showed that the plasma concentration of tectorigenin in ITME group was much higher than that of the PT group (p<0.01). Moreover, compared to PT group, t₁/₂ value and AUC(0-∞) value were also notably increased in ITME group (p<0.01). In conclusion, potential interaction exists between those chemical components in ITME, and the co-existing components in ITME could notably promote the absorption of tectoridin in rats, however, the exact compound(s) which enhance the absorption of tectoridin should be investigated in future study.
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Yang
authors.affiliation: Jiangsu Provincial Center for Research and Development of Marine Drugs, Nanjing University of Traditional Chinese Medicine, Nanjing 210029, PR China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, PR China.
authors.forename: Jinmeng
authors.initials: J
authors.lastname: An
authors.affiliation: Jiangsu Kanion Pharmaceutical Co. Ltd., Lianyungang 222001, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xiao
authors.affiliation: Jiangsu Kanion Pharmaceutical Co. Ltd., Lianyungang 222001, PR China.
authors.forename: Zhenzhong
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Jiangsu Kanion Pharmaceutical Co. Ltd., Lianyungang 222001, PR China.
authors.forename: Wenzhe
authors.initials: W
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, PR China. Electronic address: yzl1950@126.com.
authors.forename: Zhonglin
authors.initials: Z
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, PR China. Electronic address: Lifeicpu@163.com.
authors.forename: Fei
authors.initials: F
authors.lastname: Li
mesh_terms: D000284:Administration, Oral; D000818:Animals; D019540:Area Under Curve; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000032:analysis; D006868:Hydrolysis; D007498:Iris / Q000737:chemistry*; D007529:Isoflavones / Q000097:blood* / Q000737:chemistry; D044949:Kaempferols / Q000032:analysis; D057230:Limit of Detection; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry*; D011786:Quality Control; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; D007529:Isoflavones; D044949:Kaempferols; D010936:Plant Extracts; C006552:kaempferol; C120039:tectorigenin; C120040:tectoridin
keywords: Iris tectorum Maxim; Pharmacokinetics; Tectoridin; Tectorigenin; UPLC–MS/MS