title: Long non-coding RNA HOTAIR modulates c-KIT expression through sponging miR-193a in acute myeloid leukemia.
abstract: HOTAIR is significantly overexpressed in various cancers and facilitates tumor invasion and metastasis. However, whether HOTAIR plays oncogenic roles in acute myeloid leukemia (AML) is still unknown. Here, we report that HOTAIR expression was obviously increased in leukemic cell lines and primary AML blasts. Clinically, AML patients with higher HOTAIR predicted worse clinical outcome compared with those with lower HOTAIR. Importantly, HOTAIR knockdown by small hairpin RNA inhibited cell growth, induced apoptosis, and decreased number of colony formation. Finally, HOTAIR modulated c-KIT expression by competitively binding miR-193a. Collectively, our data suggest that HOTAIR plays an important oncogenic role in AML and might serve as a marker for AML prognosis and a potential target for therapeutic intervention.
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Wenzhou Medical University, Shangcai Village, Nanbaixiang, Ouhai District, Wenzhou, China.
authors.forename: Chong-yun
authors.initials: CY
authors.lastname: Xing
authors.affiliation: Surgical Oncology, The First Affiliated Hospital of Wenzhou Medical University, Shangcai Village, Nanbaixiang, Ouhai District, Wenzhou, China.
authors.forename: Xiao-qu
authors.initials: XQ
authors.lastname: Hu
authors.affiliation: Surgical Oncology, The First Affiliated Hospital of Wenzhou Medical University, Shangcai Village, Nanbaixiang, Ouhai District, Wenzhou, China.
authors.forename: Fei-yan
authors.initials: FY
authors.lastname: Xie
authors.affiliation: Laboratory of Internal Medicine, The First Affiliated Hospital of Wenzhou Medical University, Shangcai Village, Nanbaixiang, Ouhai District, Wenzhou, China.
authors.forename: Zhi-jie
authors.initials: ZJ
authors.lastname: Yu
authors.affiliation: Laboratory of Internal Medicine, The First Affiliated Hospital of Wenzhou Medical University, Shangcai Village, Nanbaixiang, Ouhai District, Wenzhou, China.
authors.forename: Hai-ying
authors.initials: HY
authors.lastname: Li
authors.affiliation: Laboratory of Internal Medicine, The First Affiliated Hospital of Wenzhou Medical University, Shangcai Village, Nanbaixiang, Ouhai District, Wenzhou, China.
authors.lastname: Bin-Zhou
authors.affiliation: Laboratory of Internal Medicine, The First Affiliated Hospital of Wenzhou Medical University, Shangcai Village, Nanbaixiang, Ouhai District, Wenzhou, China.
authors.forename: Jian-bo
authors.initials: JB
authors.lastname: Wu
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Wenzhou Medical University, Shangcai Village, Nanbaixiang, Ouhai District, Wenzhou, China.
authors.forename: Li-yuan
authors.initials: LY
authors.lastname: Tang
authors.affiliation: Laboratory of Internal Medicine, The First Affiliated Hospital of Wenzhou Medical University, Shangcai Village, Nanbaixiang, Ouhai District, Wenzhou, China. Electronic address: wzgaosm@126.com.
authors.forename: Shen-meng
authors.initials: SM
authors.lastname: Gao
mesh_terms: D045744:Cell Line, Tumor; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000473:pathology; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics / Q000502:physiology*; D011379:Prognosis; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics*; D062085:RNA, Long Noncoding / Q000378:metabolism / Q000502:physiology*; D060888:Real-Time Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: C561566:HOTAIR long untranslated RNA, human; C546818:MIRN193 microRNA, human; D035683:MicroRNAs; D062085:RNA, Long Noncoding; D008156:Luciferases; D019009:Proto-Oncogene Proteins c-kit
keywords: Acute myeloid leukemia; HOTAIR; c-KIT; miR-193a