title: Effects of inefficient transcription termination of rbcL on the expression of accD in plastids of Arabidopsis thaliana.
abstract: The plastid accD gene encodes one subunit of a multimeric acetyl-CoA carboxylase that is required for fatty acid biosynthesis. In Arabidopsis thaliana, the accD gene is transcribed by the nuclear-encoded phage-type RNA polymerase, and the accumulation of accD transcripts is subjected to a dynamic pattern during chloroplast development. However, the mechanisms underlying the regulation of accD expression remain unknown. Here, we showed that the inefficient transcription termination of rbcL due to the absence of RHON1 impaired the developmental profile of accD, resulting in the constitutive expression of accD during chloroplast development. Moreover, the accumulation of accD transcripts accordingly resulted in an increase in accD protein levels, suggesting that transcript abundance is critical for accD gene production. Our study demonstrates that the interplay between accD and upstream rbcL regulates the expression of accD and highlights the significance of transcriptional regulation in plastid gene expression in higher plants.
authors.affiliation: Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Nanxincun 20, Xiangshan, Beijing, 100093, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Baoye
authors.initials: B
authors.lastname: He
authors.affiliation: Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Nanxincun 20, Xiangshan, Beijing, 100093, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Mu
authors.affiliation: Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Nanxincun 20, Xiangshan, Beijing, 100093, China. chiweimr@ibcas.ac.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Chi
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000235:genetics*; D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics*; D002736:Chloroplasts / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D018087:Plastids / Q000235:genetics; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics*; D061806:Transcription Termination, Genetic; D014158:Transcription, Genetic
chemical_list: D029681:Arabidopsis Proteins; C101625:RbcL protein, plastid; D012273:Ribulose-Bisphosphate Carboxylase; D000103:Acetyl-CoA Carboxylase
keywords: Plastid; Plastid transcription termination; Transcriptional regulation; accD