title: Improvement of cell membrane permeability using a cell-solution electrode for generating atmospheric-pressure plasma.
abstract: The cell membrane permeability, which is strongly related to gene transfection, is improved using a cell-solution electrode for generating atmospheric-pressure plasma (APP) just above the solution. In the case of the floating cells, the cell membrane permeability is significantly improved by the cell-solution electrode APP compared with the conventional diffusion type APP, because the distance between the plasma generation area and the cell solution surface becomes short, which could reduce the radial diffusion loss of the plasma irradiated to the cell suspended solution. In the case of the adherent cells, cell membrane permeability is found to be enhanced by the shorter distance between the solution surface and the adherent cells as well as using the cell-solution electrode, which means that the short-lived reactive oxygen species generated at the solution surface are essential for the improvement of cell membrane permeability.
authors.affiliation: Department of Electronic Engineering, Tohoku University, 6-6-05 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan.
authors.forename: Toshiro
authors.initials: T
authors.lastname: Kaneko
authors.affiliation: Department of Electronic Engineering, Tohoku University, 6-6-05 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan.
authors.forename: Shota
authors.initials: S
authors.lastname: Sasaki
authors.affiliation: Department of Electronic Engineering, Tohoku University, 6-6-05 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan.
authors.forename: Yutaro
authors.initials: Y
authors.lastname: Hokari
authors.affiliation: Department of Electronic Engineering, Tohoku University, 6-6-05 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Horiuchi
authors.affiliation: Department of Electronic Engineering, Tohoku University, 6-6-05 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan.
authors.forename: Ryusuke
authors.initials: R
authors.lastname: Honda
authors.affiliation: Department of Biomedical Engineering, Tohoku University, 6-6-04 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Kanzaki
mesh_terms: D000818:Animals; D001274:Atmospheric Pressure; D018929:Cell Culture Techniques / Q000379:methods; D002463:Cell Membrane Permeability / Q000187:drug effects*; D004566:Electrodes; D005347:Fibroblasts / Q000187:drug effects* / Q000502:physiology*; D051379:Mice; D058626:Plasma Gases; D017382:Reactive Oxygen Species / Q000378:metabolism; D014162:Transfection / Q000379:methods
chemical_list: D058626:Plasma Gases; D017382:Reactive Oxygen Species