title: Differential expression of the pr1A gene in Metarhizium anisopliae and Metarhizium acridum across different culture conditions and during pathogenesis.
abstract: The entomopathogenic fungi of the genus Metarhizium have several subtilisin-like proteases that are involved in pathogenesis and these have been used to investigate genes that are differentially expressed in response to different growth conditions. The identification and characterization of these proteases can provide insight into how the fungus is capable of infecting a wide variety of insects and adapt to different substrates. In addition, the pr1A gene has been used for the genetic improvement of strains used in pest control. In this study we used quantitative RT-PCR to assess the relative expression levels of the pr1A gene in M. anisopliae and M. acridum during growth in different culture conditions and during infection of the sugar cane borer, Diatraea saccharalis Fabricius. We also carried out a pathogenicity test to assess the virulence of both species against D. saccharalis and correlated the results with the pattern of pr1A gene expression. This analysis revealed that, in both species, the pr1A gene was differentially expressed under the growth conditions studied and during the pathogenic process. M. anisopliae showed higher expression of pr1A in all conditions examined, when compared to M. acridum. Furthermore, M. anisopliae showed a greater potential to control D. saccharalis. Taken together, our results suggest that these species have developed different strategies to adapt to different growing conditions.
authors.affiliation: Departamento de Micologia, Universidade Federal de Pernambuco, Recife, PE, Brazil .
authors.forename: Mariele Porto Carneiro
authors.initials: MP
authors.lastname: Leão
authors.affiliation: Departamento de Micologia, Universidade Federal de Pernambuco, Recife, PE, Brazil .
authors.forename: Patricia Vieira
authors.initials: PV
authors.lastname: Tiago
authors.affiliation: Departamento de Ciência do Solo, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba, SP, Brazil .
authors.forename: Fernando Dini
authors.initials: FD
authors.lastname: Andreote
authors.affiliation: Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brazil .
authors.forename: Welington Luiz
authors.initials: WL
authors.lastname: de Araújo
authors.affiliation: Departamento de Micologia, Universidade Federal de Pernambuco, Recife, PE, Brazil .
authors.forename: Neiva Tinti
authors.initials: NT
authors.lastname: de Oliveira
keywords: Diatraea saccharalis; entomopathogen; expression pattern; quantitative RT-PCR