title: G-quadruplex induced chirality of methylazacalix[6]pyridine via unprecedented binding stoichiometry: en route to multiplex controlled molecular switch.
abstract: Nucleic acid based molecular device is a developing research field which attracts great interests in material for building machinelike nanodevices. G-quadruplex, as a new type of DNA secondary structures, can be harnessed to construct molecular device owing to its rich structural polymorphism. Herein, we developed a switching system based on G-quadruplexes and methylazacalix[6]pyridine (MACP6). The induced circular dichroism (CD) signal of MACP6 was used to monitor the switch controlled by temperature or pH value. Furthermore, the CD titration, Job-plot, variable temperature CD and (1)H-NMR experiments not only confirmed the binding mode between MACP6 and G-quadruplex, but also explained the difference switching effect of MACP6 and various G-quadruplexes. The established strategy has the potential to be used as the chiral probe for specific G-quadruplex recognition.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Center for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190 (P. R. China).
authors.forename: Ai-Jiao
authors.initials: AJ
authors.lastname: Guan
authors.affiliation: Department of Chemistry, Renmin University of China, Beijing, 100872 (P. R. China).
authors.forename: Meng-Jie
authors.initials: MJ
authors.lastname: Shen
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Center for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190 (P. R. China).
authors.forename: Jun-Feng
authors.initials: JF
authors.lastname: Xiang
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Center for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190 (P. R. China).
authors.forename: En-Xuan
authors.initials: EX
authors.lastname: Zhang
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Center for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190 (P. R. China).
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Center for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190 (P. R. China).
authors.forename: Hong-Xia
authors.initials: HX
authors.lastname: Sun
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Center for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190 (P. R. China).
authors.forename: Li-Xia
authors.initials: LX
authors.lastname: Wang
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Center for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190 (P. R. China).
authors.forename: Guang-Zhi
authors.initials: GZ
authors.lastname: Xu
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Center for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190 (P. R. China).
authors.forename: Ya-Lin
authors.initials: YL
authors.lastname: Tang
authors.affiliation: Department of Chemistry, Renmin University of China, Beijing, 100872 (P. R. China).
authors.forename: Li-Jin
authors.initials: LJ
authors.lastname: Xu
authors.affiliation: College of Chemistry, Beijing Normal University, Beijing, 100875 (P. R. China).
authors.forename: Han-Yuan
authors.initials: HY
authors.lastname: Gong
mesh_terms: D047250:Calixarenes / Q000737:chemistry*; D002942:Circular Dichroism; D039301:Computers, Molecular; D004247:DNA / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000737:chemistry; D054856:G-Quadruplexes; D006863:Hydrogen-Ion Concentration; D008958:Models, Molecular; D062105:Molecular Docking Simulation; D019906:Nuclear Magnetic Resonance, Biomolecular; D009841:Oligonucleotides / Q000737:chemistry*; D013696:Temperature
chemical_list: D004268:DNA-Binding Proteins; D009841:Oligonucleotides; C570637:methylazacalix(6)pyridine; D047250:Calixarenes; D004247:DNA