title: Effect of Resveratrol Supplementation on the SNARE Proteins Expression in Adipose Tissue of Stroptozotocin-Nicotinamide Induced Type 2 Diabetic Rats.
abstract: BACKGROUND
  Glucose uptake by muscles and fat cells is carried out by the GLUT4 system. Isoforms of the SNAP23, syntaxin-4 and VAMP-2 play an important role in regulating GLUT-4 trafficking and fusion in adipocytes. The changes of SNARE proteins levels and thus impaired GLUT-4 displacement can be one of the etiological causes of type 2 diabetes. Due to changes in the expression of these proteins in diabetes, the aim of this study was to investigate the effect of the natural compound resveratrol with anti-diabetic properties on impaired expression of SNARE proteins in type 2 diabetes.
  
  
  METHODS
  Forty male Wistar rats were used in this study. Type 2 diabetes was induced by administering a single dose of streptozotocin and nicotinamide. The expression of SNAP-23, syntaxin-4 and VAMP-2 proteins were assessed using real-time qRT-PCR. Also, some biochemical parameters were examined, including fasting blood glucose, insulin levels and insulin resistance.
  
  
  RESULTS
  The results of this study showed that, resveratrol supplementation increased blood insulin level, reduced the fasting blood glucose, and improved the insulin resistance. In addition, resveratrol supplementation increased the expression of SNAP-23, syntaxin-4 and VAMP-2 proteins that involved in GLUT-4 transport in adipose tissue of diabetic rats.
  
  
  CONCLUSIONS
  Final results showed that SNARE proteins expression is significantly reduced in diabetic rats and treatment with resveratrol supplementation is associated with the increased expression of these proteins.
authors.affiliation: Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran;
authors.forename: Azam
authors.initials: A
authors.lastname: Rezaei Farimani
authors.affiliation: Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan Iran;
authors.forename: Massoud
authors.initials: M
authors.lastname: Saidijam
authors.affiliation: Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan Iran;
authors.forename: Mohammad Taghi
authors.initials: MT
authors.lastname: Goodarzi
authors.affiliation: Department of Genetic and Molecular Medicine, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Yadegar Azari
authors.affiliation: Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran;
authors.forename: Soheila
authors.initials: S
authors.lastname: Asadi
authors.affiliation: Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran;
authors.forename: Sadegh
authors.initials: S
authors.lastname: Zarei
authors.affiliation: Department of Genetic and Molecular Medicine, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Nooshin
authors.initials: N
authors.lastname: Shabab
keywords: GLUT4; Resveratrol; SNAP23 Protein; Syntaxin-4 Protein; Vesicle-associated membrane Protein 2