title: Evaluation of the combined use of the recombinant Brucella abortus Omp10, Omp19 and Omp28 proteins for the clinical diagnosis of bovine brucellosis.
abstract: Currently, there are several serodiagnostic tools available for brucellosis, however, it is difficult to differentiate an active infection from vaccination. Hence, there is a great need to develop alternative means that can distinguish between these two conditions without utilizing lipopolysaccharide (LPS). This study was an attempt to determine the efficacy of combined recombinant Brucella (B.) abortus outer membrane proteins (rOmps) and individual rOmps in the serodiagnosis of brucellosis by enzyme linked immunosorbent assay (ELISA), utilizing both that standard tube agglutination test (TAT)-positive and -negative serum samples from Korean native cattle. The results are very interesting and promising because the combined rOmp antigens used in the study were highly reactive with the TAT-positive serum samples. The combined rOmps sensitivity, specificity and accuracy were 215/232 (92.67%), 294/298 (98.66%) and 509/530 (96.04%), respectively. While these results are preliminary, the tests performed have very high potential in the serodiagnosis of brucellosis and likewise, the combined rOmps can be used for future vaccine production.
authors.affiliation: Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, 660-701, Republic of Korea.
authors.forename: Hannah Leah Tadeja
authors.initials: HL
authors.lastname: Simborio
authors.affiliation: Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do, 430-757, Republic of Korea.
authors.forename: Jin Ju
authors.initials: JJ
authors.lastname: Lee
authors.affiliation: Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, 660-701, Republic of Korea.
authors.forename: Alisha Wehdnesday
authors.initials: AW
authors.lastname: Bernardo Reyes
authors.affiliation: Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, 660-701, Republic of Korea.
authors.forename: Huynh Tan
authors.initials: HT
authors.lastname: Hop
authors.affiliation: Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, 660-701, Republic of Korea.
authors.forename: Lauren Togonon
authors.initials: LT
authors.lastname: Arayan
authors.affiliation: Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, 660-701, Republic of Korea.
authors.forename: Wongi
authors.initials: W
authors.lastname: Min
authors.affiliation: Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, 660-701, Republic of Korea.
authors.forename: Hu Jang
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul, 151-742, Republic of Korea.
authors.forename: Han Sang
authors.initials: HS
authors.lastname: Yoo
authors.affiliation: Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, 660-701, Republic of Korea; Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 660-701, Republic of Korea. Electronic address: kimsuk@gnu.ac.kr.
authors.forename: Suk
authors.initials: S
authors.lastname: Kim
mesh_terms: D000372:Agglutination Tests; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology*; D002007:Brucellosis, Bovine / Q000175:diagnosis*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology*; D056910:Republic of Korea; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods*; D014730:Veterinary Medicine / Q000379:methods*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D011994:Recombinant Proteins
keywords: Brucella abortus; Outer membrane protein; Recombinant protein; Serodiagnosis