title: The expression of CXCL13 and its relation to unfavorable clinical characteristics in young breast cancer.
abstract: BACKGROUND
  Young breast cancer occupies a higher and higher proportion of breast cancer, especially in Asia, and is associated with a more unfavorable prognosis compared with the disease arising in older women. However, the poor prognosis of young breast cancer cannot be fully explained by the clinical and molecular factors.
  
  
  METHODS
  This study investigated 1125 Chinese breast cancer patients diagnosed from 2009 to 2013. A data mining of gene expression profiles was performed for the young and older breast cancer patients, identifying significantly differentially expressed genes. Quantitative RT-PCR, Western blotting and immunohistochemistry assay were carried out for the clinical sample validations.
  
  
  RESULTS
  The investigation firstly displayed that young patients (≤45 years) accounted for 47.6 % (535/1125) of breast cancer, and clinically associated with some unfavorable factors related to poor prognosis, such as invasive pathological type, high tumor grade, lymph node positive, ER negative and triple-negative subtype. Subsequently, 553 significantly differentially expressed genes were identified by the data mining. Of them, a set of genes related to immune function were observed to be up-regulated in young patients with breast cancer. Impressively, the CXCL13 (C-X-C motif chemokine 13) expression level showed the most significant difference (FC = 2.64, P = 8.2 × 10(-4)). Furthermore, the validations with clinical samples and correlation analysis demonstrated that CXCL13 was indeed highly expressed in young breast cancer and closely associated with some prognostic factors including lymph node positive and ER negative.
  
  
  CONCLUSIONS
  This is the first to indicate the clinical relevance of CXCL13 to young breast cancer and represents a potential therapeutic target for young breast cancer.
authors.affiliation: Breast Center, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, 510515, People's Republic of China. Dr.Looker@hotmail.com.
authors.forename: Lujia
authors.initials: L
authors.lastname: Chen
authors.affiliation: Cancer Research Institute and the Provincial Key Laboratory of Functional Proteomics, Southern Medical University, Guangzhou, Guangdong, 510515, People's Republic of China. zxhuang@smu.edu.cn.
authors.forename: Zhongxi
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Breast Center, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, 510515, People's Republic of China. ygy531@163.com.
authors.forename: Guangyu
authors.initials: G
authors.lastname: Yao
authors.affiliation: Department of Laboratory Medicine, the Third Affiliated Hospital, Southern Medical University, Guangzhou, Guangdong, 510630, People's Republic of China. lvxiaoming6108@126.com.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Lyu
authors.affiliation: Cancer Research Institute and the Provincial Key Laboratory of Functional Proteomics, Southern Medical University, Guangzhou, Guangdong, 510515, People's Republic of China. lzcy2008@126.com.
authors.forename: Jinbang
authors.initials: J
authors.lastname: Li
authors.affiliation: Breast Center, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, 510515, People's Republic of China. 4991132@qq.com.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Hu
authors.affiliation: Breast Center, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, 510515, People's Republic of China. choiyh@126.com.
authors.forename: Yahong
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Breast Center, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, 510515, People's Republic of China. liwenji1979@163.com.
authors.forename: Wenji
authors.initials: W
authors.lastname: Li
authors.affiliation: Cancer Research Institute and the Provincial Key Laboratory of Functional Proteomics, Southern Medical University, Guangzhou, Guangdong, 510515, People's Republic of China. xinli268@gmail.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: Breast Center, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, 510515, People's Republic of China. yechsh2014@hotmail.com.
authors.forename: Changsheng
authors.initials: C
authors.lastname: Ye
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D054382:Chemokine CXCL13 / Q000235:genetics* / Q000378:metabolism; D057225:Data Mining; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D015854:Up-Regulation / Q000235:genetics; D055815:Young Adult
chemical_list: C110663:CXCL13 protein, human; D054382:Chemokine CXCL13; D012333:RNA, Messenger