title: Hepatic metallothioneins in molecular responses to cobalt, zinc, and their nanoscale polymeric composites in frog Rana ridibunda.
abstract: Despite numerous studies suggesting a dramatic decline of amphibians, the biochemical mechanisms of adaptation in these animals to polluted environment are poorly studied. The aim of this study was to elucidate the ability to release cobalt (Co) and zinc (Zn) from their nanoscale complexes (NCs) derived from the polymeric substance of N-vinylpyrrolidone (PS) in the liver of amphibian (Rana ridibunda). Frog males were subjected to 14days exposure to waterborne Co(2+) (50μg/L), Zn(2+) (100μg/L), as well as corresponding concentrations of Co-NC, Zn-NC or PS. Main attention was paid to MT's interrelations with indices of stress and toxicity. Only Co(2+) and Zn(2+) caused elevation of the correspondent metal in MTs. Co(2+) caused down-regulation of cathepsin D activity, while Zn(2+), Zn-NC and the PS up-regulated this activity. Zn(2+) provoked 1.6 times increase of metal-bounded form of the MT (MT-Me), while all other exposures caused the elevation of the ratio of MT total protein concentration (MT-SH) and concentrations of the MT-Me and/or immunoreactive (MTi) form (up to ~10 times) accompanied by a decrease in the levels of oxyradicals. The increased DNA fragmentation and down-regulation of caspase-3 activity in relation to the redox state of glutathione and/or lactate/pyruvate were shown at all exposures. These data indicate the vulnerability of the redox state of cellular thiols and inability to release Co and Zn from NCs in frog's liver.
authors.affiliation: Ternopil National Pedagogical University (TNPU), Ternopil, Ukraine; I.Ya. Horbachevsky Ternopil State Medical University, Ternopil, Ukraine.
authors.forename: Halina
authors.initials: H
authors.lastname: Falfushynska
authors.affiliation: Ternopil National Pedagogical University (TNPU), Ternopil, Ukraine.
authors.forename: Lesya
authors.initials: L
authors.lastname: Gnatyshyna
authors.affiliation: Ternopil National Pedagogical University (TNPU), Ternopil, Ukraine.
authors.forename: Olga
authors.initials: O
authors.lastname: Fedoruk
authors.affiliation: Lviv National Polytechnic University (LNPU), Lviv, Ukraine.
authors.forename: Natalia
authors.initials: N
authors.lastname: Mitina
authors.affiliation: Lviv National Polytechnic University (LNPU), Lviv, Ukraine.
authors.forename: Alexander
authors.initials: A
authors.lastname: Zaichenko
authors.affiliation: Ternopil National Pedagogical University (TNPU), Ternopil, Ukraine.
authors.forename: Oksana
authors.initials: O
authors.lastname: Stoliar
authors.affiliation: Institute of Cell Biology (ICB), NAS of Ukraine, Lviv, Ukraine. Electronic address: stoika@cellbiol.lviv.ua.
authors.forename: Rostyslav
authors.initials: R
authors.lastname: Stoika
mesh_terms: D000818:Animals; D003035:Cobalt / Q000737:chemistry / Q000633:toxicity*; D005786:Gene Expression Regulation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D008668:Metallothionein / Q000378:metabolism*; D049329:Nanostructures / Q000737:chemistry / Q000633:toxicity*; D011898:Ranidae; D013312:Stress, Physiological / Q000187:drug effects; D015032:Zinc / Q000737:chemistry / Q000633:toxicity*
chemical_list: D003035:Cobalt; D008668:Metallothionein; D015032:Zinc
keywords: Cobalt; Metallothioneins; Nanoscale composite; Rana ridibunda; Zinc