title: OmpA family proteins and Pmp-like autotransporter: new adhesins of Waddlia chondrophila.
abstract: Waddlia chondrophila is a obligate intracellular bacterium belonging to the Chlamydiales order, a clade that also includes the well-known classical Chlamydia responsible for a number of severe human and animal diseases. Waddlia is an emerging pathogen associated with adverse pregnancy outcomes in humans and abortion in ruminants. Adhesion to the host cell is an essential prerequisite for survival of every strict intracellular bacteria and, in classical Chlamydia, this step is partially mediated by polymorphic outer membrane proteins (Pmps), a family of highly diverse autotransporters that represent about 15% of the bacterial coding capacity. Waddlia chondrophila genome however only encodes one putative Pmp-like protein. Using a proteomic approach, we identified several bacterial proteins potentially implicated in the adhesion process and we characterized their expression during the replication cycle of the bacteria. In addition, we demonstrated that the Waddlia Pmp-like autotransporter as well as OmpA2 and OmpA3, two members of the extended Waddlia OmpA protein family, exhibit adhesive properties on epithelial cells. We hypothesize that the large diversity of the OmpA protein family is linked to the wide host range of these bacteria that are able to enter and multiply in various host cells ranging from protozoa to mammalian and fish cells.
authors.affiliation: Center for Research on Intracellular Bacteria (CRIB), Institute of Microbiology, University Hospital Center and University of Lausanne, 1011 Lausanne, Switzerland.
authors.forename: Carole
authors.initials: C
authors.lastname: Kebbi-Beghdadi
authors.affiliation: Institut für Funktionelle Genomforschung der Mikroorganismen, Heinrich-Heine-Universität, 40225 Düsseldorf, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Domröse
authors.affiliation: Institut für Funktionelle Genomforschung der Mikroorganismen, Heinrich-Heine-Universität, 40225 Düsseldorf, Germany.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Becker
authors.affiliation: Center for Research on Intracellular Bacteria (CRIB), Institute of Microbiology, University Hospital Center and University of Lausanne, 1011 Lausanne, Switzerland.
authors.forename: Ousmane H
authors.initials: OH
authors.lastname: Cisse
authors.affiliation: Institut für Funktionelle Genomforschung der Mikroorganismen, Heinrich-Heine-Universität, 40225 Düsseldorf, Germany.
authors.forename: Johannes H
authors.initials: JH
authors.lastname: Hegemann
authors.affiliation: Center for Research on Intracellular Bacteria (CRIB), Institute of Microbiology, University Hospital Center and University of Lausanne, 1011 Lausanne, Switzerland gilbert.greub@chuv.ch.
authors.forename: Gilbert
authors.initials: G
authors.lastname: Greub
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002695:Chlamydiales / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D004847:Epithelial Cells / Q000382:microbiology; D006801:Humans; D000069320:Type V Secretion Systems / Q000235:genetics / Q000378:metabolism*
chemical_list: D018829:Adhesins, Bacterial; D001425:Bacterial Outer Membrane Proteins; D000069320:Type V Secretion Systems; C078167:OMPA outer membrane proteins
keywords: adhesins; autotransporter; chlamydia; intracellular bacteria; virulence