title: Changes in Functional Activity of JEG-3 Trophoblast Cell Line in the Presence of Factors Secreted by Placenta.
abstract: OBJECTIVE
  Cells in the maternal-fetal interface secrete cytokines that regulate proliferation, migration, and trophoblast invasion during the first trimester of pregnancy and the limitation of these processes during the third trimester. The aim of the study was to evaluate the influence of factors secreted by human placenta during the first and third trimester of pregnancy on cytokine receptor expression and proliferative and migratory activity of JEG-3 trophoblast cells.
  
  
  METHODS
  The research was conducted using the explant conditioned media of placentas obtained from healthy women with elective termination of pregnancy at 9-11 weeks and placentas of women whose pregnancy progressed without complications at 38-39 weeks. Assessment of surface molecule expression was performed using FACS Canto II flow cytometer (BD, USA). The proliferative activity of JEG-3 trophoblast cells was evaluated by dyeing with crystal violet vital dye. The migration activity of JEG-3 was evaluated using 24-well insert plates with polycarbonate inserts (pore size 8 microns).
  
  
  RESULTS
  Expression of CD116, CD118, CD119, IFNγ-R2, CD120b, CD183, CD192, CD295, EGFR, and TGFβ-R2 on JEG-3 was higher when the cells were incubated in the presence of the third trimester placental factors in comparison with the first trimester placental factors. Factors secreted by the placenta during the third trimester of pregnancy had more pronounced stimulatory effect on the proliferation and migration of trophoblast in comparison with baseline levels and with the effect of the first trimester placental factors.
  
  
  CONCLUSIONS
  The findings suggest that the behavior of trophoblasts in vitro might not be representative of in vivo behavior in the absence of additional local factors that influence the trophoblast in vivo.
authors.affiliation: Department of Immunology, D.O. Ott Research Institute for Obstetrics and Gynecology, Saint Petersburg, Russia.
authors.forename: Dmitry I
authors.initials: DI
authors.lastname: Sokolov
authors.affiliation: Department of Immunology, D.O. Ott Research Institute for Obstetrics and Gynecology, Saint Petersburg, Russia.
authors.forename: Ksenya N
authors.initials: KN
authors.lastname: Furaeva
authors.affiliation: Department of Immunology, D.O. Ott Research Institute for Obstetrics and Gynecology, Saint Petersburg, Russia.
authors.forename: Olga I
authors.initials: OI
authors.lastname: Stepanova
authors.affiliation: Department of Immunology, D.O. Ott Research Institute for Obstetrics and Gynecology, Saint Petersburg, Russia.
authors.forename: Olga M
authors.initials: OM
authors.lastname: Ovchinnikova
authors.affiliation: Department of Immunology, D.O. Ott Research Institute for Obstetrics and Gynecology, Saint Petersburg, Russia.
authors.forename: Larisa P
authors.initials: LP
authors.lastname: Viazmina
authors.affiliation: Department of Immunology, D.O. Ott Research Institute for Obstetrics and Gynecology, Saint Petersburg, Russia.
authors.forename: Georgy R
authors.initials: GR
authors.lastname: Kozonov
authors.affiliation: Department of Immunology, D.O. Ott Research Institute for Obstetrics and Gynecology, Saint Petersburg, Russia.
authors.forename: Tatyana U
authors.initials: TU
authors.lastname: Kuzminykh
authors.affiliation: Department of Immunology, D.O. Ott Research Institute for Obstetrics and Gynecology, Saint Petersburg, Russia. Electronic address: selkovsa@mail.ru.
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Selkov
mesh_terms: D000293:Adolescent; D000328:Adult; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D005260:Female; D006801:Humans; D008264:Macrophages / Q000378:metabolism; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy / Q000378:metabolism*; D011261:Pregnancy Trimester, First / Q000378:metabolism; D011263:Pregnancy Trimester, Third / Q000378:metabolism; D011264:Pregnancy Trimesters / Q000378:metabolism*; D018121:Receptors, Cytokine / Q000096:biosynthesis*; D014327:Trophoblasts / Q000378:metabolism; D055815:Young Adult
chemical_list: D018121:Receptors, Cytokine
keywords: Cytokine; Migration; Pregnancy; Proliferation; Receptor; Trophoblast