title: Inhibitory effects of eucalyptus and banaba leaf extracts on nonalcoholic steatohepatitis induced by a high-fructose/high-glucose diet in rats.
abstract: Nonalcoholic steatohepatitis (NASH) is a liver disease associated with metabolic syndrome. The aim of this work was to examine whether eucalyptus (Eucalyptus globulus) leaf extract (ELE) and banaba (Lagerstroemia speciosa L.) leaf extract (BLE) inhibited NASH induced by excessive ingestion of fructose in rats. Wistar rats were divided into four groups according to four distinct diets: starch diet (ST), high-fructose/high-glucose diet (FG), FG diet supplemented with ELE, or FG diet supplemented with BLE. All rats were killed after 5 weeks of treatment. Serum alanine aminotransferase and total cholesterol levels were significantly lower in the BLE group than in the FG group. Liver histopathology, including steatosis, lipogranulomas, and perisinusoidal fibrosis, was significantly attenuated in the ELE and BLE groups compared with the FG group. Levels of 2-thiobarbituric acid reactive substances (TBARS), which reflect oxidative injury to the liver, were significantly suppressed by ELE and BLE. Western blotting analysis indicated that interleukin-6 expression levels were significantly lower in the ELE and BLE groups than in the FG group. These results suggest that ELE and BLE reduced lipogenesis, oxidative stress, and inflammatory cytokine expression and thus inhibited NASH induced by excessive ingestion of fructose in rats.
authors.affiliation: Department of Pathology, Teikyo University School of Medicine, Tokyo 173-8605, Japan.
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Takahashi
authors.affiliation: Research and Development Center, Nagaoka Perfumery Co. Ltd., Ibaraki, Osaka 567-0005, Japan ; Center for Research and Development of Bioresources, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan.
authors.forename: Keiichiro
authors.initials: K
authors.lastname: Sugimoto
authors.affiliation: Department of Pathology, Teikyo University School of Medicine, Tokyo 173-8605, Japan.
authors.forename: Yurie
authors.initials: Y
authors.lastname: Soejima
authors.affiliation: Department of Pathology, Teikyo University School of Medicine, Tokyo 173-8605, Japan.
authors.forename: Arisa
authors.initials: A
authors.lastname: Kumagai
authors.affiliation: Division of Applied Life Sciences, Graduate School of Life and Environment Sciences, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan.
authors.forename: Tatsuki
authors.initials: T
authors.lastname: Koeda
authors.affiliation: Center for Research and Development of Bioresources, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan ; Department of Nutrition and Health, Faculty of Human Development, Soai University, Suminoe-ku, Osaka 559-0033, Japan.
authors.forename: Aiko
authors.initials: A
authors.lastname: Shojo
authors.affiliation: Research and Development Center, Nagaoka Perfumery Co. Ltd., Ibaraki, Osaka 567-0005, Japan.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Nakagawa
authors.affiliation: Division of Applied Life Sciences, Graduate School of Life and Environment Sciences, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Harada
authors.affiliation: Division of Applied Life Sciences, Graduate School of Life and Environment Sciences, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan.
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Yamaji
authors.affiliation: Center for Research and Development of Bioresources, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan ; Department of Clinical Nutrition, College of Health and Human Sciences, Osaka Prefecture University, Habikino, Osaka 583-8555, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Inui
authors.affiliation: Department of Internal Medicine, Teikyo University School of Medicine, Tokyo 173-8605, Japan.
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Yamanouchi
authors.affiliation: Department of Pathology, Teikyo University School of Medicine, Tokyo 173-8605, Japan.
authors.forename: Toshio
authors.initials: T
authors.lastname: Fukusato
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D004032:Diet; D005052:Eucalyptus / Q000737:chemistry*; D005247:Feeding Behavior / Q000187:drug effects; D005632:Fructose / Q000009:adverse effects*; D005947:Glucose / Q000009:adverse effects*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D018836:Inflammation Mediators / Q000378:metabolism; D031563:Lagerstroemia / Q000737:chemistry*; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D065626:Non-alcoholic Fatty Liver Disease / Q000097:blood / Q000188:drug therapy* / Q000201:enzymology; D009929:Organ Size / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D018515:Plant Leaves / Q000737:chemistry*; D017208:Rats, Wistar; D018121:Receptors, Cytokine / Q000235:genetics / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014280:Triglycerides / Q000097:blood
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D010936:Plant Extracts; D018121:Receptors, Cytokine; D017392:Thiobarbituric Acid Reactive Substances; D014280:Triglycerides; D005632:Fructose; D005954:Glucosephosphate Dehydrogenase; D005947:Glucose