title: Discrimination between streptavidin and avidin with fluorescent affinity-based probes.
abstract: Two biotinylated coumarin-based fluorescent probes SPS3 and RC3 were designed for differentiating between structurally similar proteins streptavidin (SA) and avidin (AV). A substituted phenyl group is introduced onto SPS3, which may quench the fluorescence through twist intramolecular charge transfer (TICT). The fluorescence of SPS3 is turned on, by restraining the TICT process, when the fluorophore is buried at the surface of SA. RC3 is constructed by incorporating a biotin molecule to a coumarin fluorophore through a 4-atom spacer. The fluorescence intensity of RC3 is enhanced significantly when its fluorophore enters into the less polar binding pocket of AV. SPS3 and RC3 could be applied in distinguishing between SA and AV as well as in fluorescence imaging of biotin receptor over-expressed Hela cells.
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, China. junhongqian@ecust.edu.cn weibingzhang@ecust.edu.cn.
authors.forename: Qian
authors.initials: Q
authors.lastname: Sun
authors.forename: Haiyu
authors.initials: H
authors.lastname: Tian
authors.forename: Haoran
authors.initials: H
authors.lastname: Qu
authors.forename: Deheng
authors.initials: D
authors.lastname: Sun
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Chen
authors.forename: Liping
authors.initials: L
authors.lastname: Duan
authors.forename: Weibing
authors.initials: W
authors.lastname: Zhang
authors.forename: Junhong
authors.initials: J
authors.lastname: Qian
mesh_terms: D000818:Animals; D001360:Avidin / Q000032:analysis* / Q000737:chemistry*; D002417:Cattle; D003374:Coumarins / Q000737:chemistry; D004579:Electron Transport; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D019809:Streptavidin / Q000032:analysis* / Q000737:chemistry*
chemical_list: D003374:Coumarins; D005456:Fluorescent Dyes; D001360:Avidin; D019809:Streptavidin; C030123:coumarin