title: A transportable, inexpensive electroporator for in utero electroporation.
abstract: Electroporation is a useful technique to study gene function during development but its broad application is hampered due to the expensive equipment needed. We describe the construction of a transportable, simple and inexpensive electroporator delivering square pulses with varying length and amplitude. The device was successfully used for in utero electroporation in mouse with a performance comparable to that of commercial products.
authors.affiliation: Frey Initiative Research Unit, RIKEN Quantitative Biology Center, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe, 650-0047, Japan.|Laboratory for Neocortical Development, RIKEN Center for Developmental Biology, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe, 650-0047, Japan.|Department of Molecular and Cellular Mechanisms of Neurodegeneration, Paul Flechsig Institute of Brain Research, University of Leipzig, Liebigstraβe 19, 04103, Leipzig, Germany.
authors.forename: Torsten
authors.initials: T
authors.lastname: Bullmann
authors.affiliation: Department of Molecular and Cellular Mechanisms of Neurodegeneration, Paul Flechsig Institute of Brain Research, University of Leipzig, Liebigstraβe 19, 04103, Leipzig, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Arendt
authors.affiliation: Frey Initiative Research Unit, RIKEN Quantitative Biology Center, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe, 650-0047, Japan.
authors.forename: Urs
authors.initials: U
authors.lastname: Frey
authors.affiliation: Laboratory for Neocortical Development, RIKEN Center for Developmental Biology, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe, 650-0047, Japan.
authors.forename: Carina
authors.initials: C
authors.lastname: Hanashima
keywords: cortex; electronic circuit; electroporation; hippocampus