title: Proteomic analysis of three gonad types of swamp eel reveals genes differentially expressed during sex reversal.
abstract: A variety of mechanisms are engaged in sex determination in vertebrates. The teleost fish swamp eel undergoes sex reversal naturally and is an ideal model for vertebrate sexual development. However, the importance of proteome-wide scanning for gonad reversal was not previously determined. We report a 2-D electrophoresis analysis of three gonad types of proteomes during sex reversal. MS/MS analysis revealed a group of differentially expressed proteins during ovary to ovotestis to testis transformation. Cbx3 is up-regulated during gonad reversal and is likely to have a role in spermatogenesis. Rab37 is down-regulated during the reversal and is mainly associated with oogenesis. Both Cbx3 and Rab37 are linked up in a protein network. These datasets in gonadal proteomes provide a new resource for further studies in gonadal development.
authors.affiliation: Department of Genetics, College of Life Sciences, Wuhan University, Wuhan 430072, P. R. China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Sheng
authors.affiliation: Department of Genetics, College of Life Sciences, Wuhan University, Wuhan 430072, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Genetics, College of Life Sciences, Wuhan University, Wuhan 430072, P. R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Genetics, College of Life Sciences, Wuhan University, Wuhan 430072, P. R. China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Genetics, College of Life Sciences, Wuhan University, Wuhan 430072, P. R. China.
authors.forename: Majing
authors.initials: M
authors.lastname: Luo
authors.affiliation: Department of Genetics, College of Life Sciences, Wuhan University, Wuhan 430072, P. R. China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Lei
authors.affiliation: Department of Genetics, College of Life Sciences, Wuhan University, Wuhan 430072, P. R. China.
authors.forename: Hanhua
authors.initials: H
authors.lastname: Cheng
authors.affiliation: Department of Genetics, College of Life Sciences, Wuhan University, Wuhan 430072, P. R. China.
authors.forename: Rongjia
authors.initials: R
authors.lastname: Zhou
mesh_terms: D000818:Animals; D005260:Female; D005786:Gene Expression Regulation; D006066:Gonads / Q000378:metabolism*; D008297:Male; D025941:Protein Interaction Mapping; D060066:Protein Interaction Maps; D040901:Proteomics / Q000379:methods; D019849:Sex Determination Processes; D023701:Smegmamorpha / Q000235:genetics* / Q000378:metabolism*