title: Vascular endothelial growth factor (-460 C/T, +405 G/C, and +936 C/T) polymorphisms and endometriosis risk in Tunisian population.
abstract: The vascular endothelial growth factor (VEGF), a major angiogenic factor, is known to play an important role in the development of endometriosis. The aim of this study was to investigate the association of three VEGF (-460 C/T, +405 G/C, and +936 C/T) polymorphisms with the risk of endometriosis in the Tunisian population. This study includes 105 women with endometriosis and 150 women with no laparoscopic evidence of disease. Genotyping of the VEGF -460 C/T, +405 G/C, and +936 C/T polymorphisms were performed by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). The distribution of genotypes (P = 0.006) and allele (P = 0.0009) frequencies of the +936 C/T polymorphism was significantly different between patients and controls. Patients with stages III-IV endometriosis showed a higher VEGF + 936T allele frequency than controls (P = 0.0001). However, the distribution of genotypes and allele frequencies of the VEGF -460 C/T and +405 G/C polymorphisms did not differ significantly between endometriosis patients and controls. These findings suggest that the VEGF +936 C/T polymorphism may be a risk factor for endometriosis development and the VEGF +936 T allele is associated with an increased risk of stages III-IV endometriosis in the Tunisian population.
authors.affiliation: Department of Basic Sciences, Division of Histology and Immunology, Medicine School of Tunis, Tunis El Manar University , Unit Research UR12SP15 and.
authors.forename: Belkis
authors.initials: B
authors.lastname: Henidi
authors.forename: Wajih
authors.initials: W
authors.lastname: Kaabachi
authors.forename: Abir
authors.initials: A
authors.lastname: Naouali
authors.forename: Safa
authors.initials: S
authors.lastname: Kaabachi
authors.forename: Amel
authors.initials: A
authors.lastname: Zhioua
authors.forename: Faycal
authors.initials: F
authors.lastname: Haj Sassi
authors.forename: Kamel
authors.initials: K
authors.lastname: Hamzaoui
mesh_terms: D016022:Case-Control Studies; D004715:Endometriosis / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D011110:Polymorphism, Genetic; D014416:Tunisia; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: D042461:Vascular Endothelial Growth Factor A
keywords: Endometriosis; VEGF; gene; polymorphism; vascular endothelial growth factor