title: Human Umbilical Cord Mesenchymal Stem Cells Inhibit the Function of Allogeneic Activated Vγ9Vδ2 T Lymphocytes In Vitro.
abstract: BACKGROUND
  Human umbilical cord mesenchymal stem cells (UC-MSCs) can regulate the function of immune cells. However, whether and how UC-MSCs can modulate the function of Vγ9Vδ2 T cells has not been fully understood.
  
  
  METHODS
  The PBMCs or Vγ9Vδ2 T cells were activated and expanded with pamidronate (PAM) and interleukin-2 (IL-2) with or without the presence UC-MSCs. The effects of UC-MSCs on the proliferation, cytokine expression, and cytotoxicity of Vγ9Vδ2 T cells were determined by flow cytometry. The effects of UC-MSCs on Fas-L, TRAIL-expressing Vγ9Vδ2 T cells, and Vγ9Vδ2 T cell apoptosis were determined by flow cytometry.
  
  
  RESULTS
  UC-MSCs inhibited Vγ9Vδ2 T cell proliferation in a dose-dependent but cell-contact independent manner. Coculture with UC-MSCs reduced the frequency of IFNγ+ but increased granzyme B+ Vγ9Vδ2 T cells. UC-MSCs inhibited the cytotoxicity of Vγ9Vδ2 T cells against influenza virus H1N1 infected A549 cells and also reduced the frequency of Fas-L+, TRAIL+ Vγ9Vδ2 T cells but failed to modulate the apoptosis of Vγ9Vδ2 T cells.
  
  
  CONCLUSIONS
  These results indicated that UC-MSCs efficiently suppressed the proliferation and cytotoxicity of Vγ9Vδ2 T cells and modulated their cytokine production. Fas-L and TRAIL were involved in the regulation. Cell contact and apoptosis of Vγ9Vδ2 T cells were not necessary for the inhibition.
authors.affiliation: Key Laboratory of Obstetrics & Gynecology and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second Hospital, Sichuan University, Chengdu 610041, China ; Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Xiaohuan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Key Laboratory of Obstetrics & Gynecology and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Feng
authors.affiliation: Chengdu Blood Center, Chengdu 610041, China.
authors.forename: Tianxiang
authors.initials: T
authors.lastname: Gong
authors.affiliation: Key Laboratory of Obstetrics & Gynecology and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Chongyang
authors.initials: C
authors.lastname: Shen
authors.affiliation: Key Laboratory of Obstetrics & Gynecology and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Obstetrics & Gynecology and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Qihong
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: Key Laboratory of Obstetrics & Gynecology and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Li
mesh_terms: D017209:Apoptosis; D002450:Cell Communication; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D016207:Cytokines / Q000096:biosynthesis; D053222:Fas Ligand Protein / Q000378:metabolism; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology; D007251:Influenza, Human / Q000276:immunology / Q000821:virology; D008213:Lymphocyte Activation / Q000276:immunology*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000378:metabolism; D014470:Umbilical Cord / Q000166:cytology*
chemical_list: D016207:Cytokines; D053222:Fas Ligand Protein; D016692:Receptors, Antigen, T-Cell, gamma-delta; D053221:TNF-Related Apoptosis-Inducing Ligand