title: Silver Nanoscale Hexagonal Column Chips for Detecting Cell-free DNA and Circulating Nucleosomes in Cancer Patients.
abstract: Blood tests, which are commonly used for cancer screening, generally have low sensitivity. Here, we developed a novel rapid and simple method to generate silver nanoscale hexagonal columns (NHCs) for use in surface-enhanced Raman scattering (SERS). We reported that the intensity of SERS spectra of clinical serum samples obtained from gastrointestinal cancer patients is was significantly higher than that of SERS spectra of clinical serum samples obtained from non-cancer patients. We estimated the combined constituents on silver NHCs by using a field emission-type scanning electron microscope, Raman microscopes, and a 3D laser scanning confocal microscope. We obtained the Raman scattering spectra of samples of physically fractured cells and clinical serum. No spectra were obtained for chemically lysed cultured cells and DNA, RNA, and protein extracted from cultured cells. We believe that our method, which uses SERS with silver NHCs to detect circulating nucleosomes bound by methylated cell-free DNA, may be successfully implemented in blood tests for cancer screening.
authors.affiliation: Digestive Disease Center, Showa University Koto Toyosu Hospital, Tokyo, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ito
authors.affiliation: Mytech Inc., Kobe, Japan.
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Hasegawa
authors.affiliation: Mytech Inc., Kobe, Japan.
authors.forename: Yuuki
authors.initials: Y
authors.lastname: Hasegawa
authors.affiliation: Unit of Organ Oriented Medicine, Division of Digestive and General Surgery, Department of Medicine, Ryukyu University, Okinawa, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Nishimaki
authors.affiliation: Division of Human Genetics, National Institute of Genetics, Mishima, Japan.
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Hosomichi
authors.affiliation: Department of Laboratory Medicine and Central Clinical Laboratory, Showa University Northern Yokohama Hospital, Yokohama, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kimura
authors.affiliation: Institute of Molecular Oncology, Showa University, Tokyo, Japan.
authors.forename: Motoi
authors.initials: M
authors.lastname: Ohba
authors.affiliation: Graduate School of Material Science, University of Hyogo, Hyogo, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yao
authors.affiliation: Digestive Disease Center, Showa University Koto Toyosu Hospital, Tokyo, Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Onimaru
authors.affiliation: Division of Human Genetics, National Institute of Genetics, Mishima, Japan.
authors.forename: Ituro
authors.initials: I
authors.lastname: Inoue
authors.affiliation: Digestive Disease Center, Showa University Koto Toyosu Hospital, Tokyo, Japan.
authors.forename: Haruhiro
authors.initials: H
authors.lastname: Inoue
mesh_terms: D045744:Cell Line, Tumor; D004247:DNA / Q000032:analysis* / Q000097:blood; D019175:DNA Methylation; D055088:Early Detection of Cancer; D006801:Humans; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures / Q000737:chemistry*; D009369:Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000473:pathology; D009707:Nucleosomes / Q000378:metabolism*; D012834:Silver / Q000737:chemistry*; D013059:Spectrum Analysis, Raman
chemical_list: D009707:Nucleosomes; D012834:Silver; D004247:DNA