title: Hemagglutinating/Hemolytic activities in extracts of marine invertebrates from the Brazilian coast and isolation of two lectins from the marine sponge Cliona varians and the sea cucumber Holothuria grisea.
abstract: Twenty species of marine invertebrates from the Brazilian coast were screened for hemagglutinating/hemolytic activity. In at least twelve tested species, hemagglutinating activity was different for different blood types, suggesting the presence of lectins. Extracts from four species showed hemolytic activity. Two new lectins were purified from the marine sponge Cliona varians (CvL-2) and sea cucumber Holothuria grisea (HGL). CvL-2 was able to agglutinate rabbit erythrocytes and was inhibited by galactosides. The hemagglutinating activity was optimal in pH neutral and temperatures below 70 °C. CvL-2 is a trimeric protein with subunits of 175 kDa. On the other hand, HGL showed both hemagglutinating and hemolytic activity in human and rabbit erythrocytes, but hemolysis could be inhibited by osmotic protection, and agglutination was inhibited by mucin. HGL was stable in pH values ranging from 4 to 10 and temperatures up to 90 °C. In electrophoresis and gel filtration, HGL was a monomeric protein with 15 kDa. CvL-2 and HGL showed different levels of toxicity to Artemia naplii. CvL-2 showed LC50 of 850.1 μg/mL, whereas HGL showed LC50 of 9.5 µg/mL.
authors.affiliation: Escola de Medicina, Universidade Potiguar, Natal, RN, Brasil.
authors.forename: Raniere M
authors.initials: RM
authors.lastname: Moura
authors.affiliation: Departamento de Engenharia de Pesca, Escola de Medicina, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
authors.forename: Arthur A
authors.initials: AA
authors.lastname: Melo
authors.affiliation: Departamento de Engenharia de Pesca, Escola de Medicina, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
authors.forename: Rômulo F
authors.initials: RF
authors.lastname: Carneiro
authors.affiliation: Faculdade Leão Sampaio, Juazeiro do Norte, CE, Brasil.
authors.forename: Cícera R F
authors.initials: CR
authors.lastname: Rodrigues
authors.affiliation: Departamento de Biologia Molecular, Universidade Federal da Paraíba, João Pessoa, PB, Brasil.
authors.forename: Plínio
authors.initials: P
authors.lastname: Delatorre
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
authors.forename: Kyria S
authors.initials: KS
authors.lastname: Nascimento
authors.affiliation: Departamento de Engenharia de Pesca, Escola de Medicina, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
authors.forename: Silvana
authors.initials: S
authors.lastname: Saker-Sampaio
authors.affiliation: Departamento de Engenharia de Pesca, Escola de Medicina, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
authors.forename: Celso S
authors.initials: CS
authors.lastname: Nagano
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
authors.forename: Benildo S
authors.initials: BS
authors.lastname: Cavada
authors.affiliation: Departamento de Engenharia de Pesca, Escola de Medicina, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
authors.forename: Alexandre H
authors.initials: AH
authors.lastname: Sampaio
mesh_terms: D000818:Animals; D001156:Artemia / Q000187:drug effects; D001938:Brazil; D004912:Erythrocytes / Q000187:drug effects*; D006384:Hemagglutination / Q000187:drug effects*; D006386:Hemagglutination Tests; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D037102:Lectins / Q000145:classification / Q000302:isolation & purification / Q000494:pharmacology*; D011161:Porifera / Q000737:chemistry* / Q000145:classification; D011817:Rabbits; D012616:Sea Cucumbers / Q000737:chemistry* / Q000145:classification
chemical_list: D037102:Lectins