title: Effects of postweaning administration of conjugated linoleic acid on development of obesity in nescient basic helix-loop-helix 2 knockout mice.
abstract: Conjugated linoleic acid (CLA) has been reported to prevent body weight gain and fat accumulation in part by improving physical activity in mice. However, the effects of postweaning administration of CLA on the development of obesity later in life have not yet been demonstrated. The current study investigated the role of postweaning CLA treatment on skeletal muscle energy metabolism in genetically induced inactive adult-onset obese model, nescient basic helix-loop-helix 2 knockout (N2KO) mice. Four-week-old male N2KO and wild type mice were fed either control or a CLA-containing diet (0.5%) for 4 weeks, and then CLA was withdrawn and control diet provided to all mice for the following 8 weeks. Postweaning CLA supplementation in wild type animals, but not N2KO mice, may activate AMP-activated protein kinase (AMPK) and peroxisome proliferator-activated receptor-δ (PPARδ) as well as promote desensitization of phosphatase and tensin homologue (PTEN) and sensitization of protein kinase B (AKT) at threonine 308 in gastrocnemius skeletal muscle, improving voluntary activity and glucose homeostasis. We suggest that postweaning administration of CLA may in part stimulate the underlying molecular targets involved in muscle energy metabolism to reduce weight gain in normal animals, but not in the genetically induced inactive adult-onset animal model.
authors.forename: Yoo
authors.initials: Y
authors.lastname: Kim
authors.forename: Daeyoung
authors.initials: D
authors.lastname: Kim
authors.affiliation: §Department of Human Nutrition, Foods and Exercise, Virginia Tech, Blacksburg, Virginia 24061, United States.
authors.forename: Deborah J
authors.initials: DJ
authors.lastname: Good
authors.forename: Yeonhwa
authors.initials: Y
authors.lastname: Park
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000172:deficiency / Q000235:genetics*; D019587:Dietary Supplements / Q000032:analysis; D004734:Energy Metabolism; D006801:Humans; D044243:Linoleic Acids, Conjugated / Q000008:administration & dosage*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D018482:Muscle, Skeletal / Q000254:growth & development / Q000378:metabolism; D009765:Obesity / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D051059:PTEN Phosphohydrolase / Q000235:genetics / Q000378:metabolism; D047492:Peroxisome Proliferator-Activated Receptors / Q000235:genetics / Q000378:metabolism
chemical_list: D055372:AMP-Activated Protein Kinases; D051792:Basic Helix-Loop-Helix Proteins; D044243:Linoleic Acids, Conjugated; D051059:PTEN Phosphohydrolase; D047492:Peroxisome Proliferator-Activated Receptors; C484104:Nhlh2 protein, mouse; C487757:Pten protein, mouse
keywords: AMP-activated protein kinase; childhood obesity; conjugated linoleic acid; glucose homeostasis; voluntary activity