title: A method for estimating the glomerular filtration rate in conscious monkeys.
abstract: To establish a method for estimating the glomerular filtration rate (GFR) in conscious monkeys, the radiographic contrast medium iodixanol and the standard agent inulin were coadministered as tracers to male cynomolgus monkeys (Macaca fascicularis) as a bolus injection; blood was collected after 60, 90 and 120 min. An equation based on a single-blood-sample method derived from Jacobsson's formula was prepared using the data from healthy and saline- and gentamicin-treated monkeys by a multisample strategy with iodixanol. The GFR using the equation with iodixanol was in agreement with that from the multisample method with inulin or iodixanol. When the GFR decreased to more than 60% of the basal reference level, serum creatinine concentrations tended to increase, whereas serum blood urea nitrogen concentrations fluctuated. The results suggest that the single-sample-blood method with iodixanol is a practical tool for estimating the monkey GFR in a toxicological research setting therefore minimizing animal sufferings.
authors.affiliation: Cooperative Department of Veterinary Medicine, Iwate University, 3-18-8 Ueda, Morioka, Iwate, 020-8550, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Satoh
authors.affiliation: Cooperative Department of Veterinary Medicine, Iwate University, 3-18-8 Ueda, Morioka, Iwate, 020-8550, Japan.
authors.forename: Nana
authors.initials: N
authors.lastname: Nomiya
authors.affiliation: Cooperative Department of Veterinary Medicine, Iwate University, 3-18-8 Ueda, Morioka, Iwate, 020-8550, Japan.
authors.forename: Daiki
authors.initials: D
authors.lastname: Imai
authors.affiliation: Cooperative Department of Veterinary Medicine, Iwate University, 3-18-8 Ueda, Morioka, Iwate, 020-8550, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Sato
authors.affiliation: Medicinal Safety Research Laboratories, Daiichi Sankyo Co., Ltd., 1-16-13 Kita-Kasai, Edogawa-ku, Tokyo, 134-0081, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Sakurai
authors.affiliation: Medicinal Safety Research Laboratories, Daiichi Sankyo Co., Ltd., 1-16-13 Kita-Kasai, Edogawa-ku, Tokyo, 134-0081, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Takasuna
authors.affiliation: Cooperative Department of Veterinary Medicine, Iwate University, 3-18-8 Ueda, Morioka, Iwate, 020-8550, Japan.
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Furuhama
mesh_terms: D000818:Animals; D003287:Contrast Media / Q000032:analysis*; D003404:Creatinine / Q000097:blood*; D005919:Glomerular Filtration Rate / Q000502:physiology*; D007444:Inulin / Q000097:blood*; D007677:Kidney Function Tests / Q000379:methods*; D008252:Macaca fascicularis / Q000502:physiology*; D008297:Male; D011849:Radioactive Tracers; D014283:Triiodobenzoic Acids / Q000097:blood*
chemical_list: D003287:Contrast Media; D011849:Radioactive Tracers; D014283:Triiodobenzoic Acids; D007444:Inulin; D003404:Creatinine; C044834:iodixanol
keywords: cynomolgus monkey; glomerular filtration rate; iodixanol; serum clearance