title: Microarray Expression Profile Analysis of Long Non-Coding RNAs in Umbilical Cord Plasma Reveals their Potential Role in Gestational Diabetes-Induced Macrosomia.
abstract: BACKGROUND
  Fetal macrosomia and its associated complications are the most frequent and serious morbidities for infants associated with gestational diabetes mellitus (GDM). The associations between long non-coding RNAs (lncRNAs) and macrosomia have been rarely reported; therefore, we investigated the umbilical cord lncRNA profiles in GDM macrosomia.
  
  
  METHODS
  Thirty pairs of GDM macrosomia and normal controls were divided into three subgroups randomly, and the umbilical cord vein blood from each subgroup was mixed, and hybridized to a microarray containing probes representing 33,000 lncRNA genes. Quantitative real-time polymerase chain reaction (qPCR) was used to validate selected differentially expressed lncRNAs. The gene ontology (GO), pathway and network analysis were performed.
  
  
  RESULTS
  The microarray identified 8814 lncRNAs that were expressed in the umbilical cord blood, of which 349 were significantly upregulated and 892 were significantly downregulated (fold-change ≥ 2.0) in GDM group. The highest enriched GOs targeted by downregulated transcripts were biological regulation. Pathway analysis indicated that nine pathways corresponded to downregulated transcripts.
  
  
  CONCLUSIONS
  Certain lncRNAs that were aberrantly expressed in the umbilical cord blood from GDM macrosomia might play a partial or key role in GDM macrosomia development. This study provided potential targets for treatment of macrosomia and novel insights into macrosomia biology.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Shi
authors.forename: Chun
authors.initials: C
authors.lastname: Zhao
authors.forename: Wei
authors.initials: W
authors.lastname: Long
authors.forename: Hongjuan
authors.initials: H
authors.lastname: Ding
authors.forename: Rong
authors.initials: R
authors.lastname: Shen
mesh_terms: D000328:Adult; D016640:Diabetes, Gestational / Q000097:blood / Q000235:genetics*; D005260:Female; D005312:Fetal Blood / Q000378:metabolism; D005320:Fetal Macrosomia / Q000097:blood / Q000235:genetics*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D011247:Pregnancy; D062085:RNA, Long Noncoding / Q000097:blood / Q000235:genetics*
chemical_list: D062085:RNA, Long Noncoding