title: Selective One-Dimensional Total Correlation Spectroscopy Nuclear Magnetic Resonance Experiments for a Rapid Identification of Minor Components in the Lipid Fraction of Milk and Dairy Products: Toward Spin Chromatography?
abstract: We report a rapid, direct, and unequivocal spin-chromatographic separation and identification of minor components in the lipid fraction of milk and common dairy products with the use of selective one-dimensional (1D) total correlation spectroscopy (TOCSY) nuclear magnetic resonance (NMR) experiments. The method allows for the complete backbone spin-coupling network to be elucidated even in strongly overlapped regions and in the presence of major components from 4 × 10(2) to 3 × 10(3) stronger NMR signal intensities. The proposed spin-chromatography method does not require any derivatization steps for the lipid fraction, is selective with excellent resolution, is sensitive with quantitation capability, and compares favorably to two-dimensional (2D) TOCSY and gas chromatography-mass spectrometry (GC-MS) methods of analysis. The results of the present study demonstrated that the 1D TOCSY NMR spin-chromatography method can become a procedure of primary interest in food analysis and generally in complex mixture analysis.
authors.forename: Christina
authors.initials: C
authors.lastname: Papaemmanouil
authors.forename: Constantinos G
authors.initials: CG
authors.lastname: Tsiafoulis
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Alivertis
authors.affiliation: ⊥Department of Agricultural Sciences, Biotechnology and Food Sciences, Cyprus University of Technology, Post Office Box 50329, Limassol 3603, Cyprus.
authors.forename: Ouranios
authors.initials: O
authors.lastname: Tzamaloukas
authors.affiliation: ⊥Department of Agricultural Sciences, Biotechnology and Food Sciences, Cyprus University of Technology, Post Office Box 50329, Limassol 3603, Cyprus.
authors.forename: Despoina
authors.initials: D
authors.lastname: Miltiadou
authors.forename: Andreas G
authors.initials: AG
authors.lastname: Tzakos
authors.forename: Ioannis P
authors.initials: IP
authors.lastname: Gerothanassis
mesh_terms: D000818:Animals; D002417:Cattle; D002845:Chromatography; D003611:Dairy Products / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D008055:Lipids / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation / Q000379:methods*; D008892:Milk / Q000737:chemistry*
chemical_list: D008055:Lipids
keywords: dairy products; lipid fraction; spin chromatography; totally correlated NMR spectroscopy (TOCSY)