title: Synergistic Effect of PEI and PDMAEMA on Transgene Expression in Vitro.
abstract: Polyethylenimine (PEI) and poly(2-(dimethylamino) ethyl methacrylate) (PDMAEMA) have both been used for DNA delivery. PDMAEMA has been shown to exhibit better gene transfection efficiency but lower expression ability than PEI. We mixed the two polymers at different ratios to investigate whether the resulting "dual" polyplex (PEI/PDMAEMA/DNA) could enhance both gene transfection efficiency and DNA expression ability. Experimental results showed a significant increase in DNA internalization and DNA expression for the PDMAEMA/PEI/DNA polyplexes at a ratio of 1:3 or 1:9 (PDMAEMA: PEI), depending on cell type, in comparison with PEI/DNA, PDMAEMA/DNA, and PDMAEMA/PEI/DNA at other ratios. PDMAEMA/PEI/DNA polyplexes did not reduce cell viability. In contrast to with the conventional approach using covalently modified PEI, the proposed "combination" approach provided a more convenient and effective way to improve transgene expression efficiency.
authors.affiliation: †Department of Physical Medicine and Rehabilitation, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan ROC.
authors.forename: Chia-Wen
authors.initials: CW
authors.lastname: Lo
authors.affiliation: †Department of Physical Medicine and Rehabilitation, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan ROC.
authors.forename: Wei-Hao
authors.initials: WH
authors.lastname: Liao
authors.affiliation: †Department of Physical Medicine and Rehabilitation, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan ROC.|‡Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan ROC.
authors.forename: Chueh-Hung
authors.initials: CH
authors.lastname: Wu
authors.affiliation: †Department of Physical Medicine and Rehabilitation, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan ROC.
authors.forename: Jyun-Lin
authors.initials: JL
authors.lastname: Lee
authors.affiliation: ⊥Division of Medical Engineering Research, National Health Research Institutes, Miaoli, Taiwan.
authors.forename: Ming-Kuan
authors.initials: MK
authors.lastname: Sun
authors.forename: Hui-Shan
authors.initials: HS
authors.lastname: Yang
authors.affiliation: §Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan ROC.
authors.forename: Wei-Bor
authors.initials: WB
authors.lastname: Tsai
authors.forename: Yung
authors.initials: Y
authors.lastname: Chang
authors.affiliation: †Department of Physical Medicine and Rehabilitation, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan ROC.|⊥Division of Medical Engineering Research, National Health Research Institutes, Miaoli, Taiwan.
authors.forename: Wen-Shiang
authors.initials: WS
authors.lastname: Chen
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000235:genetics*; D018014:Gene Transfer Techniques; D008689:Methacrylates / Q000737:chemistry* / Q000494:pharmacology; D051379:Mice; D015394:Molecular Structure; D041681:NIH 3T3 Cells; D009757:Nylons / Q000737:chemistry* / Q000494:pharmacology; D011094:Polyethyleneimine / Q000737:chemistry* / Q000494:pharmacology; D011401:Promoter Regions, Genetic / Q000235:genetics; D019076:Transgenes / Q000235:genetics*
chemical_list: D008689:Methacrylates; D009757:Nylons; C407037:poly(2-(dimethylamino)ethyl methacrylate); D011094:Polyethyleneimine; D004247:DNA