title: Nerve injury induces the expression of syndecan-1 heparan sulfate proteoglycan in primary sensory neurons.
abstract: Heparan sulfate proteoglycans (HSPGs) have important functions in development of the central nervous system; however, their functions in nerve injury are not yet fully understood. We previously reported the expression of syndecan-1, a type of HSPG, in cranial motor neurons after nerve injury, suggesting the importance of syndecan-1 in the pathology of motor nerve injury. In this study, we examined the expression of syndecan-1, a type of HSPG, in primary sensory neurons after nerve injury in mice. Sciatic nerve axotomy strongly induced the expression of syndecan-1 in a subpopulation of injured dorsal root ganglion (DRG) neurons, which were small in size and had CGRP- or isolectin B4-positive fibers. Syndecan-1 was also distributed in the dorsal horn of the spinal cord ipsilateral to the axotomy, and located on the membrane of axons in lamina II of the dorsal horn. Not only sciatic nerve axotomy, infraorbital nerve axotomy also induced the expression of syndecan-1 in trigeminal ganglion neurons. Moreover, syndecan-1 knockdown in cultured DRG neurons induced a shorter neurite extension. These results suggest that syndecan-1 expression in injured primary sensory neurons may have functional roles in nerve regeneration and synaptic plasticity, resulting in the development of neuropathic pain.
authors.affiliation: Department of Functional Anatomy and Neuroscience, Asahikawa Medical University, Japan. Electronic address: kmura@asahikawa-med.ac.jp.
authors.forename: K
authors.initials: K
authors.lastname: Murakami
authors.affiliation: Department of Functional Anatomy and Neuroscience, Asahikawa Medical University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
authors.affiliation: Department of Functional Anatomy and Neuroscience, Asahikawa Medical University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Bando
authors.affiliation: Department of Functional Anatomy and Neuroscience, Asahikawa Medical University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Yoshida
mesh_terms: D000818:Animals; D019771:Axotomy; D019556:COS Cells; D002522:Chlorocebus aethiops; D004195:Disease Models, Animal; D005727:Ganglia, Spinal / Q000378:metabolism* / Q000473:pathology; D019812:Heparan Sulfate Proteoglycans / Q000378:metabolism*; D008297:Male; D008807:Mice, Inbred BALB C; D059348:Peripheral Nerve Injuries / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D012584:Sciatic Nerve / Q000293:injuries; D011984:Sensory Receptor Cells / Q000378:metabolism* / Q000473:pathology; D053668:Syndecan-1 / Q000235:genetics / Q000378:metabolism*; D046509:Tissue Culture Techniques; D012668:Trigeminal Ganglion / Q000378:metabolism* / Q000473:pathology
chemical_list: D019812:Heparan Sulfate Proteoglycans; D012333:RNA, Messenger; C508242:Sdc1 protein, mouse; D053668:Syndecan-1
keywords: C-fiber; heparan sulfate proteoglycan; nerve injury; primary sensory neuron; syndecan-1