title: Immunoglobulin isotype isolated from human placental extract does not interfere in complement-mediated bacterial opsonization within the wound milieu.
abstract: The wound healing potency of an aqueous extract of placenta can be evaluated through the presence of numerous regulatory components. The presence of glycans was detected by thin layer chromatography and fluorophore-assisted carbohydrate electrophoresis. Mass spectrometric analysis revealed the existence of multiple fragments of immunoglobulin G (IgG). IgG was present in the extract at a concentration of 25.2 ± 3.97 μg/ml. IgG possesses anti-complementary activity by diverting the complement activation from target surface. Thus, effect of placental IgG on complement-bacteria interaction was investigated through classical and alternative pathway and the preparation was ascertained to be safe with respect to their interference in the process of bacterial opsonization.
authors.affiliation: Division of Structural Biology and Bioinformatics, CSIR-Indian Institute of Chemical Biology, 4, Raja S.C.Mullick Road, Jadavpur, Kolkata 7000032, India.
authors.forename: Kanika
authors.initials: K
authors.lastname: Sharma
authors.affiliation: Division of Structural Biology and Bioinformatics, CSIR-Indian Institute of Chemical Biology, 4, Raja S.C.Mullick Road, Jadavpur, Kolkata 7000032, India.
authors.forename: Debasish
authors.initials: D
authors.lastname: Bhattacharyya
keywords: ANTS, 8-aminonaphthalene-1,3,6-trisulfonate; ATP, adenosine triphosphate; BCIP, 5-Bromo 4-Chloro 3′ indolylphosphate; BHI, Brain–Heart Infusion; BSA, bovine serum albumin; CNBr, cyanogens bromide; Complement; EDTA, ethylenediamine tetra acetic acid; EGTA, ethylene glycol tetra acetic acid; G6PDH, glucose-6-phosphate dehydrogenase; Human placental extract; IgG, immunoglobulin G; Immunoglobulin; NAD+, nicotinamide adenine dinucleotide; NBT, nitro-blue tetrazolium chloride; PNGase F, peptide N-glycosidase F; Pseudomonas aeruginosa wound physiology