title: (131)I-Nimotuzumab - A potential radioimmunotherapeutic agent in treatment of tumors expressing EGFR.
abstract: The anti-EGFR antibody Nimotuzumab was radioiodinated with I-131 by Chloramine T and Iodogen methods. The (131)I-Nimotuzumab was purified and characterized by HPLC. Purified (131)I-Nimotuzumab exhibited radiochemical purity of >95% and retained good in vitro stability upto 24h at room temperature by both the methods. Cell binding studies carried out in A431 cells expressing EGF receptors showed good immunoreactivity of the product upto 5 days post radioiodination. Biodistribution studies in normal Swiss mice showed fast clearance by both renal and gastrointestinal routes with minimal thyroid uptake.
authors.affiliation: Isotope Production and Application Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India. Electronic address: kmythili@barc.gov.in.
authors.forename: Mythili
authors.initials: M
authors.lastname: Kameswaran
authors.affiliation: Isotope Production and Application Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India.
authors.forename: Grace
authors.initials: G
authors.lastname: Samuel
authors.affiliation: Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Mumbai 400 085, India.
authors.forename: Haladhar
authors.initials: H
authors.lastname: Dev Sarma
authors.affiliation: Isotope Production and Application Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India.
authors.forename: Swamirao N
authors.initials: SN
authors.lastname: Shinde
authors.affiliation: Isotope Production and Application Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India.
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Dash
authors.affiliation: Physical and Chemical Sciences, IAEA, Vienna, Austria.
authors.forename: Meera
authors.initials: M
authors.lastname: Venkatesh
mesh_terms: D000818:Animals; D061067:Antibodies, Monoclonal, Humanized / Q000493:pharmacokinetics / Q000627:therapeutic use*; D066246:ErbB Receptors / Q000378:metabolism*; D007457:Iodine Radioisotopes / Q000493:pharmacokinetics / Q000627:therapeutic use*; D051379:Mice; D009374:Neoplasms, Experimental / Q000378:metabolism / Q000532:radiotherapy*; D016499:Radioimmunotherapy; D014018:Tissue Distribution
chemical_list: D061067:Antibodies, Monoclonal, Humanized; D007457:Iodine Radioisotopes; C501466:nimotuzumab; D066246:ErbB Receptors
keywords: (131)I-Nimotuzumab; EGFR; Head and neck cancer; Radioimmunotherapy