title: Bioactive Metabolites from Mangrove Endophytic Fungus Aspergillus sp. 16-5B.
abstract: Chemical investigation of the endophytic fungus Aspergillus sp. 16-5B cultured on Czapek's medium led to the isolation of four new metabolites, aspergifuranone (1), isocoumarin derivatives (±) 2 and (±) 3, and (R)-3-demethylpurpurester A (4), together with the known purpurester B (5) and pestaphthalides A (6). Their structures were determined by analysis of 1D and 2D NMR spectroscopic data. The absolute configuration of Compound 1 was determined by comparison of the experimental and calculated electronic circular dichroism (ECD) spectra, and that of Compound 4 was revealed by comparing its optical rotation data and CD with those of the literature. The structure of Compound 6 was further confirmed by single-crystal X-ray diffraction experiment using CuKα radiation. All isolated compounds were evaluated for their α-glucosidase inhibitory activities, and Compound 1 showed significant inhibitory activity with IC50 value of 9.05 ± 0.60 μM. Kinetic analysis showed that Compound 1 was a noncompetitive inhibitor of α-glucosidase. Compounds 2 and 6 exhibited moderate inhibitory activities.
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China. liuyayue@mail2.sysu.edu.cn.
authors.forename: Yayue
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China. chensh65@mail2.sysu.edu.cn.
authors.forename: Senhua
authors.initials: S
authors.lastname: Chen
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China. liuzhaom@mail2.sysu.edu.cn.
authors.forename: Zhaoming
authors.initials: Z
authors.lastname: Liu
authors.affiliation: School of Life Sciences and Biomedical Center, Sun Yat-Sen University, 135 Xin gang West Road, Guangzhou 510275, China. luyj@mail.sysu.edu.cn.|Key Laboratory of Functional Molecules from Oceanic Microorganism, Department of Education of Guangdong Province, Sun Yat-Sen University, Guangzhou 510080, China. luyj@mail.sysu.edu.cn.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Lu
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China. xiagp@mail2.sysu.edu.cn.
authors.forename: Guoping
authors.initials: G
authors.lastname: Xia
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China. liuhj8@mail2.sysu.edu.cn.
authors.forename: Hongju
authors.initials: H
authors.lastname: Liu
authors.affiliation: School of Life Sciences and Biomedical Center, Sun Yat-Sen University, 135 Xin gang West Road, Guangzhou 510275, China. helei8688@126.com.
authors.forename: Lei
authors.initials: L
authors.lastname: He
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China. cesshzhg@mail.sysu.edu.cn.|Key Laboratory of Functional Molecules from Oceanic Microorganism, Department of Education of Guangdong Province, Sun Yat-Sen University, Guangzhou 510080, China. cesshzhg@mail.sysu.edu.cn.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: She
mesh_terms: D001230:Aspergillus / Q000737:chemistry* / Q000378:metabolism*; D018360:Crystallography, X-Ray / Q000379:methods; D049934:Isocoumarins / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D014961:X-Ray Diffraction / Q000379:methods; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D049934:Isocoumarins; D000520:alpha-Glucosidases
keywords: Aspergillus sp.; ECD; marine fungi; optical rotation; theoretical calculations; α-glucosidase inhibitor