title: Inhibitory activities of the marine streptomycete-derived compound SF2446A2 against Chlamydia trachomatis and Schistosoma mansoni.
abstract: Infectious diseases caused by chlamydia or schistosomes are a major health problem worldwide, and particularly so in developing countries. The lack of appropriate vaccines renders the search for potent natural products against these disease-causing agents an urgent endeavor. Sponge-associated actinomycetes represent a rich reservoir for natural products. Among them, members of the genus Streptomyces are capable of synthesizing an impressive array of diverse natural products with a wide variety of biological activities. The naphthacene glycoside SF2446A2 was isolated from the calcium alginate beads culture of Streptomyces sp. strain RV15 that had originally been obtained from the Mediterranean sponge Dysidea tupha. Its structure was identified by spectroscopic analysis and MS and comparison with the literature data. SF2446A2 showed inhibitory activity against Chlamydia trachomatis and was able to inhibit the primary infection in a dose-dependent manner, as well as progeny formation. Moreover, it caused disruptive effects on the surface area of Schistosoma mansoni and affected the gonads by impairing oogenesis and spermatogenesis. Our current study demonstrates that sponge-associated actinomycetes are capable of providing compounds with new pharmacological activities and with relevance to drug discovery.
authors.affiliation: Department of Microbiology, Biocenter, University of Würzburg, Würzburg, Germany.
authors.forename: Anastasija
authors.initials: A
authors.lastname: Reimer
authors.affiliation: Biomedizinisches Forschungszentrum Seltersberg, Institute for Parasitology, Justus-Liebig-Universität Giessen, Giessen, Germany.
authors.forename: Ariane
authors.initials: A
authors.lastname: Blohm
authors.affiliation: Biomedizinisches Forschungszentrum Seltersberg, Institute for Parasitology, Justus-Liebig-Universität Giessen, Giessen, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Quack
authors.affiliation: Biomedizinisches Forschungszentrum Seltersberg, Institute for Parasitology, Justus-Liebig-Universität Giessen, Giessen, Germany.
authors.forename: Christoph G
authors.initials: CG
authors.lastname: Grevelding
authors.affiliation: Department of Microbiology, Biocenter, University of Würzburg, Würzburg, Germany.
authors.forename: Vera
authors.initials: V
authors.lastname: Kozjak-Pavlovic
authors.affiliation: Department of Microbiology, Biocenter, University of Würzburg, Würzburg, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Rudel
authors.affiliation: Department of Botany II, Julius-von-Sachs Institute for Biological Sciences, University of Würzburg, Würzburg, Germany.
authors.forename: Ute
authors.initials: U
authors.lastname: Hentschel
authors.affiliation: Department of Botany II, Julius-von-Sachs Institute for Biological Sciences, University of Würzburg, Würzburg, Germany.
authors.forename: Usama Ramadan
authors.initials: UR
authors.lastname: Abdelmohsen
mesh_terms: D000617:Aminoglycosides / Q000494:pharmacology*; D000818:Animals; D002692:Chlamydia trachomatis / Q000187:drug effects*; D006367:HeLa Cells; D006801:Humans; D011161:Porifera / Q000382:microbiology*; D012550:Schistosoma mansoni / Q000187:drug effects*; D013302:Streptomyces / Q000737:chemistry*; D014871:Water Microbiology
chemical_list: D000617:Aminoglycosides; C056022:SF 2446A2