title: [Optical surface plasmon resonance biosensors in molecular fishing].
abstract: An optical biosensor employing surface plasmon resonance is a highly efficient instrument applicable for direct real time registration of molecular interactions without additional use of any labels or coupled processes. As an independent approach it is especially effective in analysis of various ligand receptor interactions. SPR-biosensors are used for validation of studies on intermolecular interactions in complex biological systems (affinity profiling of various groups of proteins, etc.). Recently, potential application of the SPR-biosensor for molecular fishing (direct affinity binding of target molecules from complex biological mixtures on the optical biosensor surface followed by their elution for identification by LC-MS/MS) has been demonstrated. Using SPR-biosensors in such studies it is possible to solve the following tasks: (a) SPR-based selection of immobilization conditions required for the most effective affinity separation of a particular biological sample; (b) SPR-based molecular fishing for subsequent protein identification by mass spectrometry; (c) SPR-based validation of the interaction of identified proteins with immobilized ligand. This review considers practical application of the SPR technology in the context of recent studies performed in the Institute of Biomedical Chemistry on molecular fishing of real biological objects.
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia; Engelhardt Institute of Molecular Biology of RAS, Moscow, Russia.
authors.forename: A S
authors.initials: AS
authors.lastname: Ivanov
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: A E
authors.initials: AE
authors.lastname: Medvedev
mesh_terms: D016229:Amyloid beta-Peptides / Q000378:metabolism; D000818:Animals; D015374:Biosensing Techniques / Q000379:methods; D002853:Chromatography, Liquid; D015786:Cytochromes b5 / Q000378:metabolism; D006801:Humans; D007510:Isatin / Q000378:metabolism; D008024:Ligands; D013058:Mass Spectrometry; D055095:Optics and Photonics / Q000295:instrumentation; D025941:Protein Interaction Mapping / Q000379:methods; D011506:Proteins / Q000032:analysis* / Q000378:metabolism; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*
chemical_list: D016229:Amyloid beta-Peptides; D008024:Ligands; D011506:Proteins; D007510:Isatin; D015786:Cytochromes b5
keywords: amyloid-binding proteins; cytochrome b5; isatin binding proteins; molecular fishing; optical surface plasmon resonance based biosensor