title: Chromosomal rearrangements involving the NTRK1 gene in colorectal carcinoma.
abstract: Chromosomal rearrangements of the NTRK1 gene, which encodes the high affinity nerve growth factor receptor (tropomyosin related kinase, TRKA), have been observed in several epithelial cancers, such as colon cancer, papillary thyroid carcinoma or non small cell lung cancer. The various NTRK1 fusions described so far lead to constitutive activation of TRKA kinase activity and are oncogenic. We further investigated here the existence and the frequency of NTRK1 gene rearrangements in colorectal cancer. Using immunohistochemistry and quantitative reverse transcriptase PCR, we analyzed a series of human colorectal cancers. We identified two TRKA positive cases over 408, with NTRK1 chromosomal rearrangements. One of these rearrangements is a TPM3-NTRK1 fusion already observed in colon cancer, while the second one is a TPR-NTRK1 fusion never described in this type of cancer. These findings further confirm that translocations in the NTRK1 gene are recurring events in colorectal cancer, although occurring at a low frequency (around 0.5%).
authors.affiliation: Centre de Recherche en Oncologie Expérimentale, Institut de Recherche Pierre Fabre, 3 Avenue Hubert Curien BP13562, 31035 Toulouse Cedex 1, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Créancier
authors.affiliation: Centre de Recherche en Oncologie Expérimentale, Institut de Recherche Pierre Fabre, 3 Avenue Hubert Curien BP13562, 31035 Toulouse Cedex 1, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Vandenberghe
authors.affiliation: Centre de Recherche en Oncologie Expérimentale, Institut de Recherche Pierre Fabre, 3 Avenue Hubert Curien BP13562, 31035 Toulouse Cedex 1, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Gomes
authors.affiliation: Centre de Recherche en Oncologie Expérimentale, Institut de Recherche Pierre Fabre, 3 Avenue Hubert Curien BP13562, 31035 Toulouse Cedex 1, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Dejean
authors.affiliation: Centre de Recherche en Oncologie Expérimentale, Institut de Recherche Pierre Fabre, 3 Avenue Hubert Curien BP13562, 31035 Toulouse Cedex 1, France.
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Blanchet
authors.affiliation: Centre Hospitalier Universitaire de Toulouse, F-31300 France.
authors.forename: Julie
authors.initials: J
authors.lastname: Meilleroux
authors.affiliation: Centre Hospitalier Universitaire de Toulouse, F-31300 France; Centre de Recherche en Cancérologie de Toulouse, Unité Mixte de Recherche 1037 INSERM - Université Toulouse III, France.
authors.forename: Rosine
authors.initials: R
authors.lastname: Guimbaud
authors.affiliation: Centre Hospitalier Universitaire de Toulouse, F-31300 France; Centre de Recherche en Cancérologie de Toulouse, Unité Mixte de Recherche 1037 INSERM - Université Toulouse III, France.
authors.forename: Janick
authors.initials: J
authors.lastname: Selves
authors.affiliation: Centre de Recherche en Oncologie Expérimentale, Institut de Recherche Pierre Fabre, 3 Avenue Hubert Curien BP13562, 31035 Toulouse Cedex 1, France. Electronic address: anna.kruczynski@pierre-fabre.com.
authors.forename: Anna
authors.initials: A
authors.lastname: Kruczynski
mesh_terms: D000818:Animals; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics*; D002277:Carcinoma / Q000737:chemistry / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000737:chemistry / Q000235:genetics* / Q000473:pathology; D050939:Gene Fusion; D015321:Gene Rearrangement; D006801:Humans; D007150:Immunohistochemistry; D008819:Mice, Nude; D008969:Molecular Sequence Data; D028861:Nuclear Pore Complex Proteins / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics; D020917:Receptor, trkA / Q000032:analysis / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D046888:Tissue Array Analysis; D014178:Translocation, Genetic; D014335:Tropomyosin / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; D028861:Nuclear Pore Complex Proteins; D011518:Proto-Oncogene Proteins; C498783:TPM3 protein, human; C073597:TPR protein, human; D014335:Tropomyosin; D020917:Receptor, trkA
keywords: Colon cancer; Gene rearrangement; NTRK1; TRKA