title: JBP485 promotes tear and mucin secretion in ocular surface epithelia.
abstract: Dry eye syndrome (DES), a multifactorial disease of the tears and ocular surface, is one of the most common ocular disorders. Tear film contains ocular mucins and is essential for maintaining the homeostasis of the wet ocular surface. Since there are a limited number of clinical options for the treatment of DES, additional novel treatments are needed to improve the clinical results. In this study, we found that placental extract-derived dipeptide (JBP485) clearly promoted the expression and secretion of gel-forming mucin 5ac (Muc5ac) in rabbit conjunctival epithelium. JBP485 also elevated the expression level of cell surface-associated mucins (Muc1/4/16) in rabbit corneal epithelium. The Schirmer tear test results indicated that JBP485 induced tear secretion in the rabbit model. Moreover, JBP485 clinically improved corneal epithelial damage in a mouse dry eye model. Thus, our data indicate that JBP485 efficiently promoted mucin and aqueous tear secretion in rabbit ocular surface epithelium and has the potential to be used as a novel treatment for DES.
authors.affiliation: 1] Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan [2] Research Center for Inflammation and Regenerative Medicine, Doshisha University, Kyoto, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Nakamura
authors.affiliation: Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
authors.forename: Yuiko
authors.initials: Y
authors.lastname: Hata
authors.affiliation: Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
authors.forename: Maho
authors.initials: M
authors.lastname: Nagata
authors.affiliation: Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
authors.forename: Norihiko
authors.initials: N
authors.lastname: Yokoi
authors.affiliation: Japan Bio Products Co., Ltd, Tokyo, Japan.
authors.forename: Shumpei
authors.initials: S
authors.lastname: Yamaguchi
authors.affiliation: Japan Bio Products Co., Ltd, Tokyo, Japan.
authors.forename: Taiichi
authors.initials: T
authors.lastname: Kaku
authors.affiliation: Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kinoshita
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004195:Disease Models, Animal; D015352:Dry Eye Syndromes / Q000378:metabolism / Q000473:pathology; D019573:Epithelium, Corneal / Q000187:drug effects* / Q000378:metabolism; D037502:Galectin 3 / Q000235:genetics / Q000378:metabolism; D051379:Mice; D055271:Mucin 5AC / Q000378:metabolism*; D018396:Mucin-1 / Q000235:genetics / Q000378:metabolism; D055264:Mucin-4 / Q000235:genetics / Q000378:metabolism; D010456:Peptides, Cyclic / Q000494:pharmacology*; D011817:Rabbits; D060888:Real-Time Polymerase Chain Reaction; D013666:Tears / Q000378:metabolism
chemical_list: D037502:Galectin 3; C412308:JBP 485; D055271:Mucin 5AC; D018396:Mucin-1; D055264:Mucin-4; D010456:Peptides, Cyclic