title: The fibroblast growth factor-2 arrests Mycobacterium avium sp. paratuberculosis growth and immunomodulates host response in macrophages.
abstract: Mycobacterium tuberculosisis (M. tb) epidemic is one of the most severe health problem worldwide, while mechanisms underlying its pathogenesis and host immune responses remain unclear. Mycobacterium avium (M. avium), a mycobacterial species related to M. tb, shares similarities with M. tb in many ways. In this study, using M. avium infection of macrophages as a model, we systematically studied the effect of fibroblast growth factor-2 (FGF-2) on M. avium infection of macrophages. Our results showed that M. avium infection could increase FGF-2 expression on both mRNA and protein levels. M. avium infection elevated TNF-α and IFN-γ production while the addition of FGF-2 could further increase TNF-α but not IFN-γ level. M. avium infection could increase the expression of oxygen/nitrogen metabolism proteins iNOS and SOD-1, and FGF-2 had additive effect on the expression of these two proteins. M. avium infection had inhibitive effect on actin expression while FGF-2 could partly counteract such inhibition. Moreover, FGF-2 could inhibit M. avium proliferation in macrophages. Our results together indicate that macrophage-secreted FGF-2 upon M. avium infection could suppress M. avium proliferation through various ways including cytokine production, enhancement of phagocytosis as well as oxygen/nitrogen metabolism.
authors.affiliation: Department of Clinical Laboratory, Kunshan First People's Hospital, Affiliated to JiangSu University, Kunshan 215300, China. Electronic address: wangjianjun0520@163.com.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, The Second Xiangya Hospital of Central South University, Changsha 410078, China. Electronic address: 527202894@qq.com.
authors.forename: Zeyou
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, Kunshan First People's Hospital, Affiliated to JiangSu University, Kunshan 215300, China. Electronic address: 343580430@qq.com.
authors.forename: Yongliang
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Department of Clinical Laboratory, Kunshan First People's Hospital, Affiliated to JiangSu University, Kunshan 215300, China. Electronic address: 365342338@qq.com.
authors.forename: Jianhong
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Clinical Laboratory, Kunshan First People's Hospital, Affiliated to JiangSu University, Kunshan 215300, China. Electronic address: 1764540915@qq.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Tang
authors.affiliation: Department of Clinical Laboratory, Kunshan First People's Hospital, Affiliated to JiangSu University, Kunshan 215300, China. Electronic address: 1148519116@qq.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Gu
authors.affiliation: Department of Pathology, ChongQing Cancer Institute, ChongQing 404100, China. Electronic address: lgxin34147@126.com.
authors.forename: Guangxin
authors.initials: G
authors.lastname: Li
mesh_terms: D045744:Cell Line, Tumor; D003598:Cytoskeletal Proteins / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D005786:Gene Expression Regulation; D054884:Host-Pathogen Interactions; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D016927:Mycobacterium avium subsp. paratuberculosis / Q000187:drug effects* / Q000254:growth & development / Q000276:immunology / Q000472:pathogenicity; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D000072105:Superoxide Dismutase-1; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D003598:Cytoskeletal Proteins; C573415:IFNG protein, human; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; C000606290:SOD1 protein, human; D014409:Tumor Necrosis Factor-alpha; D016222:Fibroblast Growth Factor 2; D009569:Nitric Oxide; D007371:Interferon-gamma; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1
keywords: Cytokines; Cytoskeleton; Fibroblast growth factor-2; Macrophage; Mycobacterium avium