title: Copper sulfide nanoparticle-decorated graphene as a catalytic amplification platform for electrochemical detection of alkaline phosphatase activity.
abstract: Copper sulfide nanoparticle-decorated graphene sheet (CuS/GR) was successfully synthesized and used as a signal amplification platform for electrochemical detection of alkaline phosphatase activity. First, CuS/GR was prepared through a microwave-assisted hydrothermal approach. The CuS/GR nanocomposites exhibited excellent electrocatalytic activity toward the oxidation of ALP hydrolyzed products such as 1-naphthol, which produced a current response. Thus, a catalytic amplification platform based on CuS/GR nanocomposite for electrochemical detection of ALP activity was designed using 1-naphthyl phosphate as a model substrate. The current response increased linearly with ALP concentration from 0.1 to 100 U L(-1) with a detection limit of 0.02 U L(-1). The assay was applied to estimate ALP activity in human serum samples with satisfactory results. This strategy may find widespread and promising applications in other sensing systems that involves ALP.
authors.affiliation: School of Chemistry and Chemical Engineering, Ningxia University, Yinchuan 750021, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Peng
authors.affiliation: School of Chemistry and Chemical Engineering, Ningxia University, Yinchuan 750021, PR China.
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Han
authors.affiliation: School of Chemistry and Chemical Engineering, Ningxia University, Yinchuan 750021, PR China.
authors.forename: Qing-Chun
authors.initials: QC
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, Ningxia Medical University, Yinchuan 750004, PR China.
authors.forename: Hui-Qin
authors.initials: HQ
authors.lastname: Yao
authors.affiliation: School of Chemistry and Chemical Engineering, Ningxia University, Yinchuan 750021, PR China. Electronic address: gaozn@nxu.edu.cn.
authors.forename: Zuo-Ning
authors.initials: ZN
authors.lastname: Gao
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood* / Q000378:metabolism; D003300:Copper / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods; D057075:Enzyme Assays / Q000379:methods; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D008958:Models, Molecular; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D013440:Sulfides / Q000737:chemistry*
chemical_list: D013440:Sulfides; D006108:Graphite; D003300:Copper; D000469:Alkaline Phosphatase
keywords: Alkaline phosphatase; Catalysis; Copper sulfide nanoparticles; Electrochemical; Graphene; Nanocomposite