title: Electron microscopic examination of podosomes induced by phorbol 12, 13 dibutyrate on the surface of A7r5 cells.
abstract: The role of myosin light chain kinase (MLCK) in inducing podosomes was examined by confocal and electron microscopy. Removal of myosin from the actin core of podosomes using blebbistatin, a myosin inhibitor, resulted in the formation of smaller podosomes. Downregulation of MLCK by the transfection of MLCK small interfering RNA (siRNA) led to the failure of podosome formation. However, ML-7, an inhibitor of the kinase activity of MLCK, failed to inhibit podosome formation. Based on our previous report (Thatcher et al. J.Pharm.Sci. 116 116-127, 2011), we outlined the important role of the actin-binding activity of MLCK in producing smaller podosomes.
authors.affiliation: Department of Anatomy, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Tanaka
authors.affiliation: Department of Cellular and Molecular Medicine, University of California San Diego, 9500 Gillman Drive 0651, La Jolla, CA 92093-0651, USA; College of Biology, Hunan University, No.1 Denggao Road, Yuelushan, Changsha, Hunan 410082, PR China.
authors.forename: Hong-Hui
authors.initials: HH
authors.lastname: Wang
authors.affiliation: Department of Nutritional Sciences, University of Kentucky, Charles T.Wethington Bldg, 900 South Limestone, Lexington, KY 40536-0200, USA.
authors.forename: Sean E
authors.initials: SE
authors.lastname: Thatcher
authors.affiliation: Department of Anatomy, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605, Japan.
authors.forename: Haruo
authors.initials: H
authors.lastname: Hagiwara
authors.affiliation: Research Institute of Pharmaceutical Sciences, Musashino University, Nishitokyo, Tokyo 202-8585, Japan.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Takano-Ohmuro
authors.affiliation: Research Institute of Pharmaceutical Sciences, Musashino University, Nishitokyo, Tokyo 202-8585, Japan. Electronic address: kkohama@makikai.jp.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kohama
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D001381:Azepines / Q000494:pharmacology; D002478:Cells, Cultured; D015536:Down-Regulation; D016253:Microscopy, Immunoelectron; D009219:Myosin-Light-Chain Kinase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D009281:Naphthalenes / Q000494:pharmacology; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology*; D000069261:Podosomes / Q000187:drug effects* / Q000235:genetics / Q000648:ultrastructure*; D011485:Protein Binding; D034741:RNA, Small Interfering; D051381:Rats
chemical_list: D000199:Actins; D001381:Azepines; D009281:Naphthalenes; D034741:RNA, Small Interfering; C070571:ML 7; D015240:Phorbol 12,13-Dibutyrate; D009219:Myosin-Light-Chain Kinase
keywords: Immunoelectron microscopy; Myosin light chain kinase; Podosome