title: Comprehensive two-dimensional liquid chromatography coupled with quadrupole time-of-flight mass spectrometry for chemical constituents analysis of tripterygium glycosides tablets.
abstract: Comprehensive two-dimensional liquid chromatography platform (LC×LC) coupled with quadrupole time-of-flight (QTOF) mass spectrometry (MS) is developed to separate, identify and relatively determine the chemical constituents of two types of tripterygium glycosides tablets (TGT). The types and relative contents of the constituents discovered in two kinds of TGT tablets were subsequently compared. C8 and C18 column were used for the separation of the first and second dimensional chromatography ((1)D and (2)D) respectively, and an integrated shift and full gradient mode was used in (2)D. Using this LC×LC-QTOF-MS platform, 92 and 132 constituents were detected in TGT preparations from Hubei and Hunan manufacturers respectively (HB-TGT and HN-TGT), most of which belonged to the diterpenoid, triterpenoid and alkaloid families. 50 and 90 compounds were unique in HB-TGT and HN-TGT, respectively, and their relative contents proportion were 52.0% and 54.2% of HB-TGT and HN-TGT, respectively. Furthermore, two TGT tablets could both lead to obvious change in biochemical parameters, oxidative stress related parameters and histopathological status to different degree. In all, the LC×LC-QTOF-MS platform offer a powerful and efficient method for characterizing, identifying and semi-quantifying chemical components in TGT preparations.
authors.affiliation: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Qu
authors.affiliation: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
authors.forename: Zhixin
authors.initials: Z
authors.lastname: Jia
authors.affiliation: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
authors.forename: Caihong
authors.initials: C
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
authors.forename: Caisheng
authors.initials: C
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China. Electronic address: zhjl@imm.ac.cn.
authors.forename: Jinlan
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000470:Alkaloids / Q000032:analysis; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002853:Chromatography, Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D006027:Glycosides / Q000032:analysis; D013058:Mass Spectrometry; D013607:Tablets / Q000737:chemistry*; D029757:Tripterygium / Q000737:chemistry*
chemical_list: D000470:Alkaloids; D004365:Drugs, Chinese Herbal; D006027:Glycosides; D013607:Tablets
keywords: Accurate mass; Comprehensive two-dimensional chromatography (LC×LC); Tripterygium glycosides tablets (TGT)