title: Genomes of diverse isolates of the marine cyanobacterium Prochlorococcus.
abstract: The marine cyanobacterium Prochlorococcus is the numerically dominant photosynthetic organism in the oligotrophic oceans, and a model system in marine microbial ecology. Here we report 27 new whole genome sequences (2 complete and closed; 25 of draft quality) of cultured isolates, representing five major phylogenetic clades of Prochlorococcus. The sequenced strains were isolated from diverse regions of the oceans, facilitating studies of the drivers of microbial diversity-both in the lab and in the field. To improve the utility of these genomes for comparative genomics, we also define pre-computed clusters of orthologous groups of proteins (COGs), indicating how genes are distributed among these and other publicly available Prochlorococcus genomes. These data represent a significant expansion of Prochlorococcus reference genomes that are useful for numerous applications in microbial ecology, evolution and oceanography.
authors.affiliation: Department of Civil and Environmental Engineering, Massachusetts Institute of Technology , Cambridge, Massachusetts, USA.
authors.forename: Steven J
authors.identifier: 0000-0002-2638-823X
authors.initials: SJ
authors.lastname: Biller
authors.affiliation: Department of Civil and Environmental Engineering, Massachusetts Institute of Technology , Cambridge, Massachusetts, USA.
authors.forename: Paul M
authors.initials: PM
authors.lastname: Berube
authors.affiliation: Department of Civil and Environmental Engineering, Massachusetts Institute of Technology , Cambridge, Massachusetts, USA ; Microbiology Graduate Program, Massachusetts Institute of Technology , Cambridge, Massachusetts, USA.
authors.forename: Jessie W
authors.initials: JW
authors.lastname: Berta-Thompson
authors.affiliation: Department of Civil and Environmental Engineering, Massachusetts Institute of Technology , Cambridge, Massachusetts, USA.
authors.forename: Libusha
authors.initials: L
authors.lastname: Kelly
authors.affiliation: Department of Civil and Environmental Engineering, Massachusetts Institute of Technology , Cambridge, Massachusetts, USA.
authors.forename: Sara E
authors.initials: SE
authors.lastname: Roggensack
authors.affiliation: Department of Civil and Environmental Engineering, Massachusetts Institute of Technology , Cambridge, Massachusetts, USA.
authors.forename: Lana
authors.initials: L
authors.lastname: Awad
authors.affiliation: Department of Biological Sciences, University of Southern Maine , Portland, Maine, USA.
authors.forename: Kathryn H
authors.initials: KH
authors.lastname: Roache-Johnson
authors.affiliation: Department of Civil and Environmental Engineering, Massachusetts Institute of Technology , Cambridge, Massachusetts, USA ; Department of Biology, Massachusetts Institute of Technology , Cambridge, Massachusetts, USA.
authors.forename: Huiming
authors.initials: H
authors.lastname: Ding
authors.affiliation: Department of Microbiology, Oregon State University , Corvallis, Oregon, USA.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Giovannoni
authors.affiliation: School of Oceanography, Center for Environmental Genomics, University of Washington , Seattle, Washington, USA.
authors.forename: Gabrielle
authors.initials: G
authors.lastname: Rocap
authors.affiliation: Department of Biological Sciences, University of Southern Maine , Portland, Maine, USA.
authors.forename: Lisa R
authors.initials: LR
authors.lastname: Moore
authors.affiliation: Department of Civil and Environmental Engineering, Massachusetts Institute of Technology , Cambridge, Massachusetts, USA ; Department of Biology, Massachusetts Institute of Technology , Cambridge, Massachusetts, USA.
authors.forename: Sallie W
authors.initials: SW
authors.lastname: Chisholm
mesh_terms: D016680:Genome, Bacterial; D023281:Genomics; D009792:Oceans and Seas; D010802:Phylogeny; D048929:Prochlorococcus / Q000235:genetics* / Q000302:isolation & purification