title: The effects of pentoxifylline and caffeic acid phenethyl ester in the treatment of d-galactosamine-induced acute hepatitis in rats.
abstract: The aim of this study was to investigate histological changes in hepatic tissue and effects of pentoxifylline (PTX) and caffeic acid phenethyl ester (CAPE) on these changes using histochemical and biochemical methods in rats, in which hepatitis was established by D-galactosamine (D-GAL). Rats were divided into five groups as follows: control group, D-GAL (24 h) group, D-GAL group, d-GAL + PTX group, and D-GAL + CAPE group. In histological evaluations, the control group showed normal appearance of the liver cells. However in the d-GAL groups, focal areas consisting of inflammatory, necrotic, and apoptotic cells were detected in parenchyma. Glycogen loss was observed in the hepatocytes localized at the periphery of lobule. It was found that number of mast cells of portal areas were significantly higher in D-GAL groups compared with other groups (p = 0.0001). In addition, the number of cells with positive staining by Ki-67 and caspase-3 were significantly increased in GAL groups compared with the control group (p = 0.0001). In biochemical analysis, there was an increase in malondialdehyde and myeloperoxidase levels, while a decrease was observed in glutathione level and glutathione peroxidase activity in groups treated with d-GAL compared with the control group. On the other hand, it was seen that, in the groups treated with D-GAL, histological and biochemical injuries in the liver were reduced by administration of PTX and CAPE. In this study, we demonstrated the ameliorative effects of PTX and CAPE on D-GAL-induced liver injury.
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Inonu University, Malatya, Turkey eliftaslidere@hotmail.com.
authors.forename: E
authors.initials: E
authors.lastname: Taslidere
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Inonu University, Malatya, Turkey.
authors.forename: N
authors.initials: N
authors.lastname: Vardi
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Bezmialem University, Istanbul, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Esrefoglu
authors.affiliation: Department of Chemistry, Faculty of Science and Art, Inonu University, Malatya, Turkey.
authors.forename: B
authors.initials: B
authors.lastname: Ates
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Inonu University, Malatya, Turkey.
authors.forename: C
authors.initials: C
authors.lastname: Taskapan
authors.affiliation: Department of Biostatistics, Faculty of Medicine, Inonu University, Malatya, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Yologlu
mesh_terms: D000818:Animals; D002109:Caffeic Acids / Q000494:pharmacology*; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000473:pathology; D005688:Galactosamine / Q000633:toxicity*; D006003:Glycogen / Q000378:metabolism; D019394:Ki-67 Antigen / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008407:Mast Cells / Q000502:physiology; D010431:Pentoxifylline / Q000494:pharmacology*; D009195:Peroxidase / Q000378:metabolism; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002109:Caffeic Acids; D019394:Ki-67 Antigen; D008315:Malondialdehyde; D005688:Galactosamine; D006003:Glycogen; D009195:Peroxidase; D053148:Caspase 3; C055494:caffeic acid phenethyl ester; D010626:Phenylethyl Alcohol; D010431:Pentoxifylline
keywords: Hepatitis; caffeic acid phenethyl ester; d-galactosamine; pentoxifylline