title: Vitamin D metabolites and bone mineral density: The multi-ethnic study of atherosclerosis.
abstract: Previous studies demonstrate associations of low 25-hydroxyvitamin D (25(OH)D) concentrations with low bone mineral density (BMD) and fractures, motivating widespread use of vitamin D supplements for bone health. However, previous studies have been limited to predominantly White populations despite differences in the distribution and metabolism of 25(OH)D by race/ethnicity. We determined associations of serum 25(OH)D, 24,25-dihydroxyvitamin D (24,25(OH2)D3), and parathyroid hormone (PTH) with BMD among 1773 adult participants in the Multi-Ethnic Study of Atherosclerosis (MESA) in a staggered cross-sectional study design. Vitamin D metabolites were measured using liquid chromatography-mass spectroscopy and PTH using a 2-site immunoassay from serum collected in 2000-2002. Volumetric trabecular lumbar BMD was measured from computed tomography scans performed in 2002-2005 expressed as g/cm(3). We used linear regression and graphical methods to compare associations of vitamin D metabolite and PTH concentrations with BMD as the outcomes measure among White (n=714), Black (n=353), Chinese (n=249), and Hispanic (n=457) participants. Serum 25(OH)D and 24,25(OH2)D3 concentrations were highest among Whites and lowest among Blacks. BMD was greatest among Black participants. Higher serum 25(OH)D was only associated with higher BMD among Whites and Chinese participants (P-for-interaction=0.054). Comparing the lowest category of 25(OH)D (<20 ng/ml) to the highest (≥30 ng/ml), the adjusted mean difference in BMD was -8.1g/cm3 (95% CI -14.8, -1.4) for Whites; -10.2g/cm3 (-20.4, 0.0) for Chinese vs. 8.8 g/cm3 (-2.8, 20.5) for Black and -1.1g/cm3 (-8.3, 6.2) for Hispanic. Similar results were observed for serum 24,25(OH2)D3. Serum PTH was not associated with BMD. In a multi-ethnic population, associations of 25(OH)D with BMD were strongest among White and Chinese participants and null among Black and Hispanic participants. Further studies are needed to determine optimal biomarkers for bone health for multiple ethnic groups.
authors.affiliation: University of Washington, Kidney Research Institute, Seattle, WA, USA. Electronic address: hvb2@uw.edu.
authors.forename: Adriana J
authors.initials: AJ
authors.lastname: van Ballegooijen
authors.affiliation: University of Washington, Kidney Research Institute, Seattle, WA, USA.
authors.forename: Cassianne
authors.initials: C
authors.lastname: Robinson-Cohen
authors.affiliation: University of Washington, Kidney Research Institute, Seattle, WA, USA.
authors.forename: Ronit
authors.initials: R
authors.lastname: Katz
authors.affiliation: Department of Medicine, University of California San Diego, San Diego, CA, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Criqui
authors.affiliation: Department of Medicine, University of California San Diego, San Diego, CA, USA.
authors.forename: Matthew
authors.initials: M
authors.lastname: Budoff
authors.affiliation: Los Angeles Biomedical Research Institute at Harbor-UCLA, Los Angeles, CA, USA.
authors.forename: Dong
authors.initials: D
authors.lastname: Li
authors.affiliation: New York Academy of Medicine, New York, NY, USA.
authors.forename: David
authors.initials: D
authors.lastname: Siscovick
authors.affiliation: University of Washington, Kidney Research Institute, Seattle, WA, USA; Department of Laboratory Medicine, University of Washington, Seattle, WA, USA.
authors.forename: Andy
authors.initials: A
authors.lastname: Hoofnagle
authors.affiliation: Department of Epidemiology, Columbia University, New York, NY, USA.
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Shea
authors.affiliation: Department of Public Health Sciences, Wake Forest University, Winston-Salem, NC, USA.
authors.forename: Gregory
authors.initials: G
authors.lastname: Burke
authors.affiliation: University of Washington, Kidney Research Institute, Seattle, WA, USA.
authors.forename: Ian H
authors.initials: IH
authors.lastname: de Boer
authors.affiliation: University of Washington, Kidney Research Institute, Seattle, WA, USA.
authors.forename: Bryan
authors.initials: B
authors.lastname: Kestenbaum
mesh_terms: D001741:Black or African American; D000368:Aged; D000369:Aged, 80 and over; D050197:Atherosclerosis / Q000097:blood / Q000208:ethnology* / Q000503:physiopathology; D044383:Black People; D015519:Bone Density; D001842:Bone and Bones / Q000473:pathology; D002853:Chromatography, Liquid; D003430:Cross-Sectional Studies; D005006:Ethnicity; D005260:Female; D017102:Fracture Healing; D050723:Fractures, Bone / Q000150:complications; D006630:Hispanic or Latino; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D010281:Parathyroid Hormone / Q000097:blood; D012044:Regression Analysis; D014481:United States; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism*; D014808:Vitamin D Deficiency / Q000097:blood; D044465:White People
chemical_list: D010281:Parathyroid Hormone; D014807:Vitamin D; C104450:25-hydroxyvitamin D
keywords: Aging; General population studies; Parathyroid hormone; Quantitation of bone; Vitamin D