title: Expanding the MDx toolbox for filarial diagnosis and surveillance.
abstract: Filarial parasites are tissue-dwelling nematodes responsible for some of the most important neglected tropical diseases. All are transmitted by blood-sucking arthropod. Onchocerciasis and lymphatic filariasis in particular are the cause of much disfigurement and morbidity. Accurate parasite detection is essential for the success of filariasis control programs. The current toolbox for diagnosis and surveillance is limited because many of the available tools suffer from lack of sensitivity and specificity, and/or are cost-prohibitive. We review the methods currently in use and discuss the prospects for developing new molecular diagnostic (MDx) tools based on nucleic acid detection. We briefly describe recent developments in isothermal nucleic acid amplification and detection, and focus on emerging technologies that are field-deployable or suitable for low-resource settings.
authors.affiliation: Division of Genome Biology, New England Biolabs, Ipswich, MA 01938, USA.
authors.forename: Andy
authors.initials: A
authors.lastname: Alhassan
authors.affiliation: Division of Genome Biology, New England Biolabs, Ipswich, MA 01938, USA.
authors.forename: Zhiru
authors.initials: Z
authors.lastname: Li
authors.affiliation: Division of Genome Biology, New England Biolabs, Ipswich, MA 01938, USA.
authors.forename: Catherine B
authors.initials: CB
authors.lastname: Poole
authors.affiliation: Division of Genome Biology, New England Biolabs, Ipswich, MA 01938, USA. Electronic address: carlow@neb.com.
authors.forename: Clotilde K S
authors.initials: CK
authors.lastname: Carlow
mesh_terms: D000818:Animals; D005368:Filariasis / Q000175:diagnosis* / Q000469:parasitology / Q000517:prevention & control*; D006801:Humans; D007159:Immunologic Tests; D025202:Molecular Diagnostic Techniques / Q000592:standards / Q000639:trends*; D021141:Nucleic Acid Amplification Techniques; D011159:Population Surveillance
keywords: filariasis; isothermal amplification; molecular diagnostics