title: Protective Effects of Pyridoxamine Against UVC-induced Programmed Cell Death in HaCaT Cells.
abstract: BACKGROUND
  Exposure to ultraviolet (UV) light is closely related to human diseases, such as skin cancer, due to irreversible injuries to the skin cells. The UV-induced DNA damage and programmed cell death are important determinants for skin carcinogenesis. The aim of the present study was to investigate the anti-ultraviolet-C (UVC) effects of pyridoxamine in human keratinocyte HaCaT cells and its mechanisms of action.
  
  
  RESULTS
  UVC-induced programmed cell death in HaCaT cells was abrogated by treated the cells immediately after UVC irradiation with 40, 80 and 160 μM of pyridoxamine. Monitoring the UVC-induced-specific reactive oxygen species, we found that 20, 40, 80 and 160 μM of pyridoxamine was also effective in suppressing the induction of reactive oxygen species by UVC.
  
  
  CONCLUSIONS
  Overall, our results provided evidence showing that pyridoxamine was effective in protecting HaCaT cells from UVC-induced programmed cell death and may be a potential anti-UVC agent in life and clinical practice.
authors.affiliation: Department of Medical Imaging and Radiological Sciences, Central-Taiwan University of Science and Technology, Taichung, Taiwan, R.O.C. Department of Internal Medicine, Division of Pulmonary and Critical Care Medicine, Taichung Armed Forces General Hospital, Taichung, Taiwan, R.O.C.
authors.forename: Shou-Cheng
authors.initials: SC
authors.lastname: Wang
authors.affiliation: Terry Fox Cancer Research Laboratory, China Medical University and Hospital, Taichung, Taiwan, R.O.C. Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan, R.O.C.
authors.forename: Hong-Xue
authors.initials: HX
authors.lastname: Ji
authors.affiliation: Terry Fox Cancer Research Laboratory, China Medical University and Hospital, Taichung, Taiwan, R.O.C. Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan, R.O.C.
authors.forename: Chieh-Lun
authors.initials: CL
authors.lastname: Hsiao
authors.affiliation: Terry Fox Cancer Research Laboratory, China Medical University and Hospital, Taichung, Taiwan, R.O.C.
authors.forename: Tzu-Chia
authors.initials: TC
authors.lastname: Wang
authors.affiliation: Terry Fox Cancer Research Laboratory, China Medical University and Hospital, Taichung, Taiwan, R.O.C.
authors.forename: Yun-Ru
authors.initials: YR
authors.lastname: Syu
authors.affiliation: Terry Fox Cancer Research Laboratory, China Medical University and Hospital, Taichung, Taiwan, R.O.C.
authors.forename: Chia-En
authors.initials: CE
authors.lastname: Miao
authors.affiliation: Terry Fox Cancer Research Laboratory, China Medical University and Hospital, Taichung, Taiwan, R.O.C.
authors.forename: Lin-Lin
authors.initials: LL
authors.lastname: Hou
authors.affiliation: Department of Medical Imaging and Radiological Sciences, Central-Taiwan University of Science and Technology, Taichung, Taiwan, R.O.C.
authors.forename: Song-Shei
authors.initials: SS
authors.lastname: Lin
authors.affiliation: Terry Fox Cancer Research Laboratory, China Medical University and Hospital, Taichung, Taiwan, R.O.C. Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan, R.O.C.
authors.forename: Wen-Shin
authors.initials: WS
authors.lastname: Chang
authors.affiliation: Terry Fox Cancer Research Laboratory, China Medical University and Hospital, Taichung, Taiwan, R.O.C. artbau2@gmail.com wenwen816@gmail.com.
authors.forename: Chia-Wen
authors.initials: CW
authors.lastname: Tsai
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D004353:Drug Evaluation, Preclinical; D006801:Humans; D011733:Pyridoxamine / Q000494:pharmacology*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D011837:Radiation-Protective Agents; D017382:Reactive Oxygen Species; D011733:Pyridoxamine
keywords: Pyridoxamine; UVC; cyclobutane pyrimidine dimer; keratinocytes; reactive oxygen species