title: Xylose fermentation by Saccharomyces cerevisiae using endogenous xylose-assimilating genes.
abstract: OBJECTIVE
  To genetically engineer Saccharomyces cerevisiae for improved ethanol productivity from glucose/xylose mixtures.
  
  
  RESULTS
  An endogenous gene cassette composed of aldose reductase (GRE3), sorbitol dehydrogenase (SOR1) and xylulose kinase (XKS1) with a PGK1 promoter and a terminator was introduced into two S. cerevisiae strains, a laboratory strain (CEN.PK2-1C) and an industrial strain (Kyokai No. 7). The engineered Kyokai No. 7 strain (K7-XYL) exhibited a higher sugar consumption rate (1.03 g l(-1) h(-1)) and ethanol yield (63.8 %) from a glucose and xylose mixture compared to the engineered CEN.PK2-1C strain. Furthermore, K7-XYL produced a larger amount of ethanol (39.6 g l(-1)) compared to K7-SsXYL (32 g l(-1)) with integrated xylose reductase and xylitol dehydrogenase from a xylose-assimilating yeast Scheffersomyces stipitis instead of GRE3 and SOR1.
  
  
  CONCLUSIONS
  The created S. cerevisiae strain showed sufficient xylose-fermenting ability to be used for efficient ethanol production from glucose/xylose.
authors.affiliation: Bio Fuels & Chemicals R&D Group, Frontier Research Laboratory, Central Technical Research Laboratory, JX Nippon Oil & Energy Corporation, 8, Chidori-cho, Naka-Ku, Yokohama, 231-0815, Japan, jin.konishi@noe.jx-group.co.jp.
authors.forename: Jin
authors.initials: J
authors.lastname: Konishi
authors.forename: Akira
authors.initials: A
authors.lastname: Fukuda
authors.forename: Kozue
authors.initials: K
authors.lastname: Mutaguchi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Uemura
mesh_terms: D000449:Aldehyde Reductase / Q000235:genetics / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D007064:L-Iditol 2-Dehydrogenase / Q000235:genetics / Q000378:metabolism; D060847:Metabolic Engineering; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D061806:Transcription Termination, Genetic; D014994:Xylose / Q000378:metabolism*
chemical_list: D000431:Ethanol; D014994:Xylose; D007064:L-Iditol 2-Dehydrogenase; D000449:Aldehyde Reductase; D017853:Phosphotransferases (Alcohol Group Acceptor); C032700:xylulokinase