title: A Plant Immune Receptor Detects Pathogen Effectors that Target WRKY Transcription Factors.
abstract: Defense against pathogens in multicellular eukaryotes depends on intracellular immune receptors, yet surveillance by these receptors is poorly understood. Several plant nucleotide-binding, leucine-rich repeat (NB-LRR) immune receptors carry fusions with other protein domains. The Arabidopsis RRS1-R NB-LRR protein carries a C-terminal WRKY DNA binding domain and forms a receptor complex with RPS4, another NB-LRR protein. This complex detects the bacterial effectors AvrRps4 or PopP2 and then activates defense. Both bacterial proteins interact with the RRS1 WRKY domain, and PopP2 acetylates lysines to block DNA binding. PopP2 and AvrRps4 interact with other WRKY domain-containing proteins, suggesting these effectors interfere with WRKY transcription factor-dependent defense, and RPS4/RRS1 has integrated a "decoy" domain that enables detection of effectors that target WRKY proteins. We propose that NB-LRR receptor pairs, one member of which carries an additional protein domain, enable perception of pathogen effectors whose function is to target that domain.
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK. Electronic address: panagiotis.sarris@tsl.ac.uk.
authors.forename: Panagiotis F
authors.initials: PF
authors.lastname: Sarris
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK.
authors.forename: Zane
authors.initials: Z
authors.lastname: Duxbury
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK.
authors.forename: Sung Un
authors.initials: SU
authors.lastname: Huh
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK.
authors.forename: Yan
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Bio-protection Research Centre, Institute of Agriculture and Environment, Massey University, Private Bag 11222, Palmerston North 4442, New Zealand.
authors.forename: Cécile
authors.initials: C
authors.lastname: Segonzac
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK.
authors.forename: Jan
authors.initials: J
authors.lastname: Sklenar
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK.
authors.forename: Paul
authors.initials: P
authors.lastname: Derbyshire
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK.
authors.forename: Volkan
authors.initials: V
authors.lastname: Cevik
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK.
authors.forename: Ghanasyam
authors.initials: G
authors.lastname: Rallapalli
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK.
authors.forename: Simon B
authors.initials: SB
authors.lastname: Saucet
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK.
authors.forename: Lennart
authors.initials: L
authors.lastname: Wirthmueller
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK.
authors.forename: Frank L H
authors.initials: FLH
authors.lastname: Menke
authors.affiliation: Bio-protection Research Centre, Institute of Agriculture and Environment, Massey University, Private Bag 11222, Palmerston North 4442, New Zealand.
authors.forename: Kee Hoon
authors.initials: KH
authors.lastname: Sohn
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK. Electronic address: jonathan.jones@tsl.ac.uk.
authors.forename: Jonathan D G
authors.initials: JDG
authors.lastname: Jones
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000276:immunology* / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000378:metabolism*; D001426:Bacterial Proteins / Q000276:immunology; D007113:Immunity, Innate; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D011551:Pseudomonas fluorescens / Q000378:metabolism / Q000472:pathogenicity; D044224:Pseudomonas syringae / Q000276:immunology / Q000378:metabolism; D014026:Nicotiana / Q000276:immunology / Q000382:microbiology
chemical_list: D029681:Arabidopsis Proteins; C098294:AvrRps4 protein, Pseudomonas syringae; D001426:Bacterial Proteins; D010940:Plant Proteins; C582898:RRS1 protein, Arabidopsis; C097421:rps4 protein, plant