title: IL-10 supplementation increases Tregs and decreases hypertension in the RUPP rat model of preeclampsia.
abstract: OBJECTIVE
  The reduced uterine perfusion pressure (RUPP) rat model of preeclampsia was used to determine the effects of added interleukin-10 (IL-10) on Tregs and hypertension in response to placental ischemia and how the decrease in these anti-inflammatory factors mediates the pathophysiology of preeclampsia.
  
  
  METHODS
  IL-10 (2.5 ng/kg/d) was infused via osmotic mini-pump implanted intraperitoneally on day 14 of gestation and, at the same time, the RUPP procedure was performed.
  
  
  RESULTS
  IL-10 reduced mean arterial pressure (p < 0.001), decreased CD4(+) T cells (p = 0.044), while increasing Tregs (p = 0.043) which led to lower IL-6 and TNF-α (p = 0.008 and p = 0.003), reduced AT1-AA production (p < 0.001), and decreased oxidative stress (p = 0.029) in RUPP rats.
  
  
  CONCLUSIONS
  These data indicate that IL-10 supplementation increases Tregs and helps to balance the altered immune system seen during preeclampsia.
authors.affiliation: Pharmacology, University of Mississippi Medical Center , Jackson, MS , USA .
authors.forename: Ashlyn
authors.initials: A
authors.lastname: Harmon
authors.forename: Denise
authors.initials: D
authors.lastname: Cornelius
authors.forename: Lorena
authors.initials: L
authors.lastname: Amaral
authors.forename: Adrienne
authors.initials: A
authors.lastname: Paige
authors.forename: Florian
authors.initials: F
authors.lastname: Herse
authors.forename: Tarek
authors.initials: T
authors.lastname: Ibrahim
authors.forename: Gerd
authors.initials: G
authors.lastname: Wallukat
authors.forename: Jessica
authors.initials: J
authors.lastname: Faulkner
authors.forename: Janae
authors.initials: J
authors.lastname: Moseley
authors.forename: Ralf
authors.initials: R
authors.lastname: Dechend
authors.forename: Babbette
authors.initials: B
authors.lastname: LaMarca
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D004195:Disease Models, Animal; D005260:Female; D006973:Hypertension / Q000097:blood / Q000188:drug therapy* / Q000503:physiopathology; D016753:Interleukin-10 / Q000494:pharmacology* / Q000627:therapeutic use; D015850:Interleukin-6 / Q000097:blood; D007511:Ischemia / Q000188:drug therapy / Q000378:metabolism; D010920:Placenta / Q000098:blood supply / Q000378:metabolism; D011225:Pre-Eclampsia / Q000097:blood / Q000188:drug therapy* / Q000503:physiopathology; D011247:Pregnancy; D017207:Rats, Sprague-Dawley; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10
keywords: Hypertension; inflammation; pregnancy.