title: Gossypetin ameliorates ionizing radiation-induced oxidative stress in mice liver--a molecular approach.
abstract: Radioprotective action of gossypetin (GTIN) against gamma (γ)-radiation-induced oxidative stress in liver was explored in the present article. Our main aim was to evaluate the protective efficacy of GTIN against radiation-induced alteration of liver in murine system. To evaluate the effect of GTIN, it was orally administered to mice at a dose of 30 mg/kg body weight for three consecutive days prior to γ-radiation at a dose of 5 Gy. Radioprotective efficacy of GTIN were evaluated at physiological, cellular, and molecular level using biochemical analysis, comet assay, flow cytometry, histopathology, immunofluorescence, and immunoblotting techniques. Ionizing radiation was responsible for augmentation of hepatic oxidative stress in terms of lipid peroxidation and depletion of endogenous antioxidant enzymes. Immunoblotting and immunofluorescence studies showed that irradiation enhanced the nuclear translocation of nuclear factor kappa B (NF-κB) level, which leads to hepatic inflammation. To investigate further, we found that radiation induced the activation of stress-activated protein kinase/c-Jun NH2-terminal kinase (SAPK/JNK)-mediated apoptotic pathway and deactivation of the NF-E2-related factor 2 (Nrf2)-mediated redox signaling pathway, whereas GTIN pretreatment ameliorated these radiation-mediated effects. This is the novel report where GTIN rationally validated the molecular mechanism in terms of the modulation of cellular signaling system' instead of ' This is the novel report where GTIN is rationally validated in molecular terms to establish it as promising radioprotective agents. This might be fruitful especially for nuclear workers and defense personnel assuming the possibility of radiation exposure.
authors.affiliation: a Department of Physiology , DST-PURSE & UGC-CPEPA supported Department, Centre for Research in Nanoscience and Nanotechnology (CRNN), University of Calcutta , Kolkata , West Bengal , India.
authors.forename: Amitava
authors.initials: A
authors.lastname: Khan
authors.forename: Krishnendu
authors.initials: K
authors.lastname: Manna
authors.forename: Dipesh Kr
authors.initials: DK
authors.lastname: Das
authors.forename: Swaraj Bandhu
authors.initials: SB
authors.lastname: Kesh
authors.forename: Mahuya
authors.initials: M
authors.lastname: Sinha
authors.forename: Ujjal
authors.initials: U
authors.lastname: Das
authors.forename: Sushobhan
authors.initials: S
authors.lastname: Biswas
authors.forename: Aaveri
authors.initials: A
authors.lastname: Sengupta
authors.forename: Kunal
authors.initials: K
authors.lastname: Sikder
authors.forename: Sanjukta
authors.initials: S
authors.lastname: Datta
authors.forename: Mahua
authors.initials: M
authors.lastname: Ghosh
authors.forename: Anindita
authors.initials: A
authors.lastname: Chakrabarty
authors.forename: Asoke
authors.initials: A
authors.lastname: Banerji
authors.forename: Sanjit
authors.initials: S
authors.lastname: Dey
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D001219:Aspartate Aminotransferases / Q000097:blood; D001682:Biological Availability; D002374:Catalase / Q000378:metabolism; D053903:DNA Breaks, Double-Stranded; D004353:Drug Evaluation, Preclinical; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D005720:Gamma Rays / Q000009:adverse effects*; D005978:Glutathione / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects / Q000528:radiation effects; D015850:Interleukin-6 / Q000097:blood; D015227:Lipid Peroxidation / Q000187:drug effects / Q000528:radiation effects; D008099:Liver / Q000187:drug effects* / Q000528:radiation effects / Q000648:ultrastructure; D008297:Male; D051379:Mice; D015394:Molecular Structure; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D011837:Radiation-Protective Agents / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D015398:Signal Transduction / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D016166:Free Radical Scavengers; D015850:Interleukin-6; D051267:NF-E2-Related Factor 2; C495636:Nfe2l2 protein, mouse; D011837:Radiation-Protective Agents; D014409:Tumor Necrosis Factor-alpha; C577686:interleukin-6, mouse; D002374:Catalase; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D019869:Phosphatidylinositol 3-Kinases; C514862:Akt1 protein, mouse; D051057:Proto-Oncogene Proteins c-akt; D000469:Alkaline Phosphatase; D005978:Glutathione; C059922:gossypetin
keywords: antioxidant; cell signaling; gamma radiation; gossypetin; inflammation; lipid peroxidation