title: Toward Whole-Transcriptome Editing with CRISPR-Cas9.
abstract: Targeted regulation of gene expression holds huge promise for biomedical research. In a series of recent publications (Gilbert et al., 2014; Konermann et al., 2015; Zalatan et al., 2015), sophisticated, multiplex-compatible transcriptional activator systems based on the CRISPR-Cas9 technology and genome-scale libraries advance the field toward whole-transcriptome control.
authors.affiliation: Department of Pediatric Hematology and Oncology, Hannover Medical School, Hannover 30625, Germany. Electronic address: heckl.dirk@mh-hannover.de.
authors.forename: Dirk
authors.initials: D
authors.lastname: Heckl
authors.affiliation: Department of Regulation in Infection Biology, Helmholtz Centre for Infection Research, Braunschweig 38124, Germany; The Laboratory for Molecular Infection Medicine Sweden (MIMS), Department of Molecular Biology, Umeå Centre for Microbial Research (UCMR), Umeå University, Umeå 90187, Sweden; Department of Regulation in Infection Biology, Hannover Medical School, Hannover 30625, Germany. Electronic address: emmanuelle.charpentier@helmholtz-hzi.de.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Charpentier
mesh_terms: D064113:CRISPR-Cas Systems / Q000235:genetics*; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D005821:Genetic Techniques; D015894:Genome, Human / Q000235:genetics*; D006801:Humans; D008545:Melanoma / Q000235:genetics*; D014158:Transcription, Genetic; D015533:Transcriptional Activation / Q000235:genetics*