title: DNA Barcoding Survey of Anurans across the Eastern Cordillera of Colombia and the Impact of the Andes on Cryptic Diversity.
abstract: Colombia hosts the second highest amphibian species diversity on Earth, yet its fauna remains poorly studied, especially using molecular genetic techniques. We present the results of the first wide-scale DNA barcoding survey of anurans of Colombia, focusing on a transect across the Eastern Cordillera. We surveyed 10 sites between the Magdalena Valley to the west and the eastern foothills of the Eastern Cordillera, sequencing portions of the mitochondrial 16S ribosomal RNA and cytochrome oxidase subunit 1 (CO1) genes for 235 individuals from 52 nominal species. We applied two barcode algorithms, Automatic Barcode Gap Discovery and Refined Single Linkage Analysis, to estimate the number of clusters or "unconfirmed candidate species" supported by DNA barcode data. Our survey included ~7% of the anuran species known from Colombia. While barcoding algorithms differed slightly in the number of clusters identified, between three and ten nominal species may be obscuring candidate species (in some cases, more than one cryptic species per nominal species). Our data suggest that the high elevations of the Eastern Cordillera and the low elevations of the Chicamocha canyon acted as geographic barriers in at least seven nominal species, promoting strong genetic divergences between populations associated with the Eastern Cordillera.
authors.affiliation: Departamento de Zoologia, Universidade de Brasília, 70910-900, Brasília, DF, Brazil.
authors.forename: Carlos E
authors.initials: CE
authors.lastname: Guarnizo
authors.affiliation: Department of Biological Sciences, Universidad de los Andes, A.A. 4976, Bogotá, Colombia.
authors.forename: Andrea
authors.initials: A
authors.lastname: Paz
authors.affiliation: Department of Biological Sciences, Universidad de los Andes, A.A. 4976, Bogotá, Colombia; Department of Basic Sciences, Universidad de la Salle, Bogotá, Colombia.
authors.forename: Astrid
authors.initials: A
authors.lastname: Muñoz-Ortiz
authors.affiliation: Department of Biological Sciences, Universidad de los Andes, A.A. 4976, Bogotá, Colombia.
authors.forename: Sandra V
authors.initials: SV
authors.lastname: Flechas
authors.affiliation: Department of Biological Sciences, Universidad de los Andes, A.A. 4976, Bogotá, Colombia.
authors.forename: Javier
authors.initials: J
authors.lastname: Méndez-Narváez
authors.affiliation: Department of Biological Sciences, Universidad de los Andes, A.A. 4976, Bogotá, Colombia; Smithsonian Tropical Research Institute, Apartado, 0843-03092, Panamá, Republic of Panama.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Crawford
mesh_terms: D000818:Animals; D001001:Anura / Q000235:genetics*; D003105:Colombia; D058893:DNA Barcoding, Taxonomic; D014644:Genetic Variation; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D012336:RNA, Ribosomal, 16S