title: Genome Engineering in Cyanobacteria: Where We Are and Where We Need To Go.
abstract: Genome engineering of cyanobacteria is a promising area of development in order to produce fuels, feedstocks, and value-added chemicals in a sustainable way. Unfortunately, the current state of genome engineering tools for cyanobacteria lags far behind those of model organisms such as Escherichia coli and Saccharomyces cerevisiae. In this review, we present the current state of synthetic biology tools for genome engineering efforts in the most widely used cyanobacteria strains and areas that need concerted research efforts to improve tool development. Cyanobacteria pose unique challenges to genome engineering efforts because their cellular biology differs significantly from other eubacteria; therefore, tools developed for other genera are not directly transferrable. Standardized parts, such as promoters and ribosome binding sites, which control gene expression, require characterization in cyanobacteria in order to have fully predictable results. The application of these tools to genome engineering efforts is also discussed; the ability to do genome-wide searching and to introduce multiple mutations simultaneously is an area that needs additional research in order to enable fast and efficient strain engineering.
authors.affiliation: Chemical and Biological Engineering Department, Colorado School of Mines , Golden, Colorado 80401, United States.
authors.forename: C Josh
authors.initials: CJ
authors.lastname: Ramey
authors.affiliation: Chemical and Biological Engineering Department, Colorado School of Mines , Golden, Colorado 80401, United States.
authors.forename: Ángel
authors.initials: Á
authors.lastname: Barón-Sola
authors.affiliation: Chemical and Biological Engineering Department, Colorado School of Mines , Golden, Colorado 80401, United States.
authors.forename: Hanna R
authors.initials: HR
authors.lastname: Aucoin
authors.affiliation: Chemical and Biological Engineering Department, Colorado School of Mines , Golden, Colorado 80401, United States.
authors.forename: Nanette R
authors.initials: NR
authors.lastname: Boyle
mesh_terms: D000458:Cyanobacteria / Q000235:genetics*; D005818:Genetic Engineering; D016680:Genome, Bacterial; D011499:Protein Processing, Post-Translational; D058615:Synthetic Biology