title: Effects of electrolysis by low-amperage electric current on the chlorophyll fluorescence characteristics of Microcystis aeruginosa.
abstract: Effects of electrolysis by low-amperage electric current on the chlorophyll fluorescence characteristics of Microcystis aeruginosa were investigated in order to reveal the mechanisms of electrolytic inhibition of algae. Threshold of current density was found under a certain initial no. of algae cell. When current density was equal to or higher than the threshold (fixed electrolysis time), growth of algae was inhibited completely and the algae lost the ability to survive. Effect of algal solution volume on algal inhibition was insignificant. Thresholds of current density were 8, 10, 14, 20, and 22 mA cm(-2) at 2.5 × 10(7), 5 × 10(7), 1 × 10(8), 2.5 × 10(8), and 5 × 10(8) cells mL(-1) initial no. of algae cell, respectively. Correlativity between threshold of current and initial no. of algae cells was established for scale-up and determining operating conditions. Changes of chlorophyll fluorescence parameters demonstrated that photosystem (PS) II of algae was damaged by electrolysis but still maintained relatively high activity when algal solution was treated by current densities lower than the threshold. The activity of algae recovered completely after 6 days of cultivation. On the contrary, when current density was higher than the threshold, connection of phycobilisome (PBS) and PS II core complexes was destroyed, PS II system of algae was damaged irreversibly, and algae could not survive thoroughly. The inactivation of M. aeruginosa by electrolysis can be attributed to irreversible separation of PBS from PS II core complexes and the damage of PS II of M. aeruginosa.
authors.affiliation: Department of Basin Water Environmental Research, Changjiang River Scientific Research Institute, Wuhan, 430010, China. linli1229@hotmail.com.|Key Laboratory of Water Resources in River Basins and Eco-Environmental Science of Hubei Province, Wuhan, 430010, China. linli1229@hotmail.com.
authors.forename: Li
authors.initials: L
authors.lastname: Lin
authors.affiliation: Department of Basin Water Environmental Research, Changjiang River Scientific Research Institute, Wuhan, 430010, China.|Key Laboratory of Water Resources in River Basins and Eco-Environmental Science of Hubei Province, Wuhan, 430010, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Feng
authors.affiliation: Department of Basin Water Environmental Research, Changjiang River Scientific Research Institute, Wuhan, 430010, China.|Key Laboratory of Water Resources in River Basins and Eco-Environmental Science of Hubei Province, Wuhan, 430010, China.
authors.forename: Qingyun
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Basin Water Environmental Research, Changjiang River Scientific Research Institute, Wuhan, 430010, China.|Key Laboratory of Water Resources in River Basins and Eco-Environmental Science of Hubei Province, Wuhan, 430010, China.
authors.forename: Min
authors.initials: M
authors.lastname: Wu
authors.affiliation: Department of Basin Water Environmental Research, Changjiang River Scientific Research Institute, Wuhan, 430010, China.|Key Laboratory of Water Resources in River Basins and Eco-Environmental Science of Hubei Province, Wuhan, 430010, China.
authors.forename: Liangyuan
authors.initials: L
authors.lastname: Zhao
mesh_terms: D002734:Chlorophyll / Q000737:chemistry* / Q000378:metabolism; D004553:Electric Conductivity; D004572:Electrolysis; D046931:Microcystis / Q000737:chemistry* / Q000201:enzymology / Q000378:metabolism; D045332:Photosystem II Protein Complex / Q000378:metabolism; D013050:Spectrometry, Fluorescence
chemical_list: D045332:Photosystem II Protein Complex; D002734:Chlorophyll
keywords: Algae; Chlorophyll fluorescence; Electrolysis; Eutrophication; Mechanism; Thresholds of current density