title: Photodynamic inactivation of bacteria using novel electrogenerated porphyrin-fullerene C60 polymeric films.
abstract: A porphyrin-fullerene C60 dyad (TCP-C60) substituted by carbazoyl groups was used to obtain electrogenerated polymeric films on optically transparent indium tin oxide (ITO) electrodes. This approach produced stable and reproducible polymers, holding fullerene units. The properties of this film were compared with those formed by layers of TCP/TCP-C60 and TCP/ZnTCP. Absorption spectra of the films presented the Soret and Q bands of the corresponding porphyrins. The TCP-C60 film produced a high photodecomposition of 2,2-(anthracene-9,10-diyl)bis(methylmalonate), which was used to detect singlet molecular oxygen O2((1)Δg) production in water. In addition, the TCP-C60 film induced the reduction of nitro blue tetrazolium to diformazan in the presence of NADH, indicating the formation of superoxide anion radical. Moreover, photooxidation of L-tryptophan mediated by TCP-C60 films was found in water. In biological media, photoinactivation of Staphylococcus aureus was evaluated depositing a drop with 2.5 × 10(3) cells on the films. After 30 min irradiation, no colony formation was detected using TCP-C60 or TCP/TCP-C60 films. Furthermore, photocytotoxic activity was observed in cell suspensions of S. aureus and Escherichia coli. The irradiated TCP-C60 film produced a 4 log decrease of S. aureus survival after 30 min. Also, a 4 log reduction of E. coli viability was obtained using the TCP-C60 film after 60 min irradiation. Therefore, the TCP-C60 film is an interesting and versatile photodynamic active surface to eradicate bacteria.
authors.affiliation: Departamento de Química, Facultad de Ciencias Exactas Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Agencia Postal Nro 3, X5804BYA Río Cuarto, Córdoba, Argentina.
authors.forename: M Belén
authors.initials: MB
authors.lastname: Ballatore
authors.affiliation: Departamento de Química, Facultad de Ciencias Exactas Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Agencia Postal Nro 3, X5804BYA Río Cuarto, Córdoba, Argentina.
authors.forename: Javier
authors.initials: J
authors.lastname: Durantini
authors.affiliation: Departamento de Química, Facultad de Ciencias Exactas Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Agencia Postal Nro 3, X5804BYA Río Cuarto, Córdoba, Argentina.
authors.forename: Natalia S
authors.initials: NS
authors.lastname: Gsponer
authors.affiliation: Departamento de Química, Facultad de Ciencias Exactas Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Agencia Postal Nro 3, X5804BYA Río Cuarto, Córdoba, Argentina.
authors.forename: María B
authors.initials: MB
authors.lastname: Suarez
authors.affiliation: Departamento de Química, Facultad de Ciencias Exactas Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Agencia Postal Nro 3, X5804BYA Río Cuarto, Córdoba, Argentina.
authors.forename: Miguel
authors.initials: M
authors.lastname: Gervaldo
authors.affiliation: Departamento de Química, Facultad de Ciencias Exactas Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Agencia Postal Nro 3, X5804BYA Río Cuarto, Córdoba, Argentina.
authors.forename: Luis
authors.initials: L
authors.lastname: Otero
authors.affiliation: Departamento de Química, Facultad de Ciencias Exactas Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Agencia Postal Nro 3, X5804BYA Río Cuarto, Córdoba, Argentina.
authors.forename: Mariana B
authors.initials: MB
authors.lastname: Spesia
authors.affiliation: Departamento de Química, Facultad de Ciencias Exactas Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Agencia Postal Nro 3, X5804BYA Río Cuarto, Córdoba, Argentina.
authors.forename: M Elisa
authors.initials: ME
authors.lastname: Milanesio
authors.affiliation: Departamento de Química, Facultad de Ciencias Exactas Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Agencia Postal Nro 3, X5804BYA Río Cuarto, Córdoba, Argentina.
authors.forename: Edgardo N
authors.initials: EN
authors.lastname: Durantini
mesh_terms: D004560:Electricity; D004566:Electrodes; D004926:Escherichia coli / Q000187:drug effects*; D037741:Fullerenes / Q000737:chemistry*; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects*; D010084:Oxidation-Reduction / Q000187:drug effects; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011108:Polymers / Q000737:chemistry*; D011166:Porphyrins / Q000737:chemistry*; D013057:Spectrum Analysis; D013211:Staphylococcus aureus / Q000187:drug effects*; D013997:Time Factors; D014364:Tryptophan / Q000378:metabolism
chemical_list: C558421:C60-porphyrin; D037741:Fullerenes; D017319:Photosensitizing Agents; D011108:Polymers; D011166:Porphyrins; D014364:Tryptophan