title: Colorimetric detection of clinical DNA samples using an intercalator-conjugated polydiacetylene sensor.
abstract: We herein developed a novel colorimetric polydiacetylene (PDA) sensor for very convenient detection of clinical DNA samples based on the interaction between an intercalator and dsDNA. We modified the terminal carboxyl group of a diacetylene monomer (10,12-pentacosadiynoic acid; PCDA) with the intercalator 9-aminoacridine (9AA) and prepared 9AA-modified PDA liposomes containing PCDA-9AA/PCDA/phospholipid (1,2-dimyristoyl-rac-glycero-3-phosphocholine) at a molar ratio of 1.5:6.5:2.0. The PDA sensor underwent an obvious color transition from blue to red in the presence of dsDNA molecules that were PCR-amplified from genomic DNA due to the insertion of the 9AA head group of PDA into the dsDNA. DNA concentrations as low as 20 nM and relatively small molecules (around 100 base pairs) could be detected by the sensor within 1h without DNA electrophoresis. This novel colorimetric method is simple, does not require any instrument, and is therefore appropriate for POCT or portable molecular diagnostic kit.
authors.affiliation: Department of Chemical and Biomolecular Engineering (BK21+Program), KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea; School of Natural Science, Ulsan National Institute of Science and Technology, Ulsan 689-798, Republic of Korea.
authors.forename: Yun Kyung
authors.initials: YK
authors.lastname: Jung
authors.affiliation: Department of Chemical and Biomolecular Engineering (BK21+Program), KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea. Electronic address: hgpark@kaist.ac.kr.
authors.forename: Hyun Gyu
authors.initials: HG
authors.lastname: Park
mesh_terms: D000585:Aminacrine / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods; D003124:Colorimetry / Q000379:methods*; D004396:Coloring Agents / Q000737:chemistry*; D004247:DNA / Q000032:analysis*; D005231:Fatty Acids, Unsaturated / Q000737:chemistry; D007364:Intercalating Agents / Q000737:chemistry*; D057230:Limit of Detection; D008081:Liposomes / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D000078789:Polyacetylene Polymer; D011108:Polymers / Q000737:chemistry*; D053279:Polyynes / Q000737:chemistry*; D013053:Spectrophotometry / Q000379:methods
chemical_list: C493048:10, 12-pentacosadiynoic acid; D004396:Coloring Agents; D005231:Fatty Acids, Unsaturated; D007364:Intercalating Agents; D008081:Liposomes; D010743:Phospholipids; D000078789:Polyacetylene Polymer; D011108:Polymers; D053279:Polyynes; C082361:polydiacetylene; D000585:Aminacrine; D004247:DNA
keywords: 9-Aminoacridine; Colorimetric biosensor; DNA detection; Intercalation; Polydiacetylene (PDA)