title: A luminescence switch-on probe for terminal deoxynucleotidyl transferase (TdT) activity detection by using an iridium(III)-based i-motif probe.
abstract: An iridium(III) complex exhibiting higher responce towards i-motif DNA over dsDNA and ssDNA was employed for the construction of a TdT activity detection platform. The assay exhibited a linear signal enhancement for TdT in the concentration range of 0 to 8 U mL(-1), and the limit of detection for TdT was 0.25 U mL(-1).
authors.affiliation: Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China. edmondma@hkbu.edu.hk.
authors.forename: Lihua
authors.initials: L
authors.lastname: Lu
authors.forename: Modi
authors.initials: M
authors.lastname: Wang
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Liu
authors.forename: Chun-Yuen
authors.initials: CY
authors.lastname: Wong
authors.forename: Chung-Hang
authors.initials: CH
authors.lastname: Leung
authors.forename: Dik-Lung
authors.initials: DL
authors.lastname: Ma
mesh_terms: D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004253:DNA Nucleotidylexotransferase / Q000378:metabolism*; D057075:Enzyme Assays / Q000379:methods*; D007495:Iridium / Q000737:chemistry*; D049408:Luminescent Agents / Q000737:chemistry*; D008163:Luminescent Measurements; D059372:Nucleotide Motifs; D009942:Organometallic Compounds / Q000737:chemistry; D010618:Phenanthrolines / Q000737:chemistry
chemical_list: D049408:Luminescent Agents; D009942:Organometallic Compounds; D010618:Phenanthrolines; D007495:Iridium; D004247:DNA; D004253:DNA Nucleotidylexotransferase