title: Rapid screening of aflatoxin B1 in beer by fluorescence polarization immunoassay.
abstract: This manuscript describes the development of a sensitive, fast and easily-performed fluorescence polarization immunoassay (FPIA) for the mycotoxin aflatoxin B1 (AFB1) in various beer samples, both lager and dark. The highest sensitivity was determined for six poly- and monoclonal antibodies selective towards aflatoxins. The sample pretreatment design was emphasized since beer samples are characterized by extremely diverse matrices. Herein, the choice of sorbent for effective removal of matrix interferences prior to analysis was crucial. The samples were diluted with a borate buffer solution containing 1% PEG 6000 and passed through the clean-up column packed with NH2-derivated silica. This sample pretreatment technique was perfectly suitable for the FPIA of lager beer samples, but for dark beer and ale it did not suffice. An artificial matrix was constructed to plot a calibration curve and quantify the results of the latter samples. The developed immunoassay was characterized by a limit of detection of 1 ng mL(-1). Apparent recovery values of 89-114% for lager and 80-125% for dark beer were established. The FPIA data for AFB1 was characterized by elevated linear regression coefficients, 0.9953 for spiked lager and 0.9895 for dark beer samples respectively.
authors.affiliation: Ghent University, Faculty of Pharmaceutical Sciences, Laboratory of Food Analysis, Harelbekestraat 72, 9000 Ghent, Belgium. Electronic address: beloglazovanv@gmail.com.
authors.forename: N V
authors.initials: NV
authors.lastname: Beloglazova
authors.affiliation: M.V. Lomonosov Moscow State University, Faculty of Chemistry, Department of Chemical Enzymology, Leninsky Gory 1, 119991 Moscow, Russia; A.N. Bach Institute of Biochemistry of the Russian Academy of Sciences, Leninsky prospect 33, 119071 Moscow, Russia.
authors.forename: S A
authors.initials: SA
authors.lastname: Eremin
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D000906:Antibodies / Q000276:immunology; D001515:Beer / Q000032:analysis*; D001881:Borates / Q000737:chemistry; D002021:Buffers; D016231:Fluorescence Polarization Immunoassay; D005506:Food Contamination / Q000032:analysis*; D011092:Polyethylene Glycols / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D000906:Antibodies; D001881:Borates; D002021:Buffers; D011092:Polyethylene Glycols; D012822:Silicon Dioxide; D016604:Aflatoxin B1
keywords: Aflatoxin B1; Beer; Fluorescence polarization immunoassay; Mycotoxins; Sample pretreatment.; Solid phase extraction