title: Encapsulation of 10-hydroxy camptothecin in supramolecular hydrogel as an injectable drug delivery system.
abstract: 10-Hydroxy camptothecin (HCPT) has been proven to be a cell cycle-specific chemotherapeutic agent, which is a necessary choice to inhibit tumor residue growth and prevent tumor metastasis after surgery. But it suffers from light decomposition, poor solubility, relatively low bioavailability, and some side effects, which are the major obstacles toward its clinical use. Integration of hydrophobic HCPT with hydrophilic hydrogel is a facile approach to change the disadvantageous situation of HCPT. In this study, a novel supramolecular hydrogelator with improved synthetic strategy was triggered by chemical hydrolysis, and then self-assembled to hydrogel. Taking advantage of the high-equilibrium solubility of HCPT in hydrogelator solution, this hydrogel was utilized to load HCPT via encapsulation as an effective carrier. HCPT hydrogels were characterized by several techniques including transmission electronic microscopy, rheology, and UV spectroscopy. In vitro release experiment indicated HCPT hydrogel could maintain long term and sustained release of HCPT at high accumulated rate. 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay showed that HCPT hydrogel had an optimized anticancer efficacy. Besides, with prominent physical properties of carrier, HCPT hydrogel possessed satisfactory stability, syringeability, and recoverability, demonstrating itself as a potential localized injectable drug delivery system.
authors.affiliation: Department of Analytical Chemistry, China Pharmaceutical University, Nanjing, 210009, People's Republic of China.|Key Laboratory of Biomedical Functional Materials, China Pharmaceutical University, Nanjing, 210009, People's Republic of China.
authors.forename: Ruixin
authors.initials: R
authors.lastname: Li
authors.affiliation: Department of Analytical Chemistry, China Pharmaceutical University, Nanjing, 210009, People's Republic of China.|Key Laboratory of Biomedical Functional Materials, China Pharmaceutical University, Nanjing, 210009, People's Republic of China.
authors.forename: Chang
authors.initials: C
authors.lastname: Shu
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Polymer Science and Engineering, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, People's Republic of China.
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, People's Republic of China.
authors.forename: Danke
authors.initials: D
authors.lastname: Xu
authors.affiliation: Department of Analytical Chemistry, China Pharmaceutical University, Nanjing, 210009, People's Republic of China.|Key Laboratory of Biomedical Functional Materials, China Pharmaceutical University, Nanjing, 210009, People's Republic of China.
authors.forename: Wenying
authors.initials: W
authors.lastname: Zhong
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry; D002166:Camptothecin / Q000737:chemistry*; D045744:Cell Line, Tumor; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods; D004355:Drug Stability; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D061986:MCF-7 Cells; D012995:Solubility
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D020100:Hydrogels; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D002166:Camptothecin
keywords: 10-hydroxy camptothecin; Cancer chemotherapy; Drug delivery systems; Encapsulation; Hydrogels; Peptides; chemical hydrolysis; injectable drug delivery; supramolecular hydrogel