title: Raman spectroscopy as a diagnostic tool for monitoring acute nephritis.
abstract: Both acute nephritis and chronic nephritis account for substantial morbidity and mortality worldwide, partly due to the lack of reliable tools for detecting disease early and monitoring its progression non-invasively. In this work, Raman spectroscopy coupled with multivariate analysis are employed for the first time to study the accelerated progression of nephritis in anti-GBM mouse model. Preliminary results show up to 98% discriminant accuracy for the severe and midly diseased and the healthy among two strains of mice with different susceptibility to acute glomerulonephritis. This technique has the potential for non-invasive or minimally-invasive early diagnosis, prognosis, and monitoring of renal disease progression.
authors.affiliation: Department of Electrical and Computer Engineering, USA.
authors.forename: Jingting
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Biomedical Engineering, USA.
authors.forename: Yong
authors.initials: Y
authors.lastname: Du
authors.affiliation: Department of Electrical and Computer Engineering, USA.
authors.forename: Ji
authors.initials: J
authors.lastname: Qi
authors.affiliation: Department of Biomedical Engineering, USA.
authors.forename: Ravikumar
authors.initials: R
authors.lastname: Sneha
authors.affiliation: Department of Pathology, The University of Chicago, USA.
authors.forename: Anthony
authors.initials: A
authors.lastname: Chang
authors.affiliation: Department of Biomedical Engineering, USA. wshih@uh.edu.
authors.forename: Chandra
authors.initials: C
authors.lastname: Mohan
authors.affiliation: Department of Electrical and Computer Engineering, USA. wshih@uh.edu.|Department of Biomedical Engineering, USA. wshih@uh.edu.|Department of Chemistry, University of Houston, 4800, Calhoun, Rd. Houston, TX, 77204, USA. wshih@uh.edu.
authors.forename: Wei-Chuan
authors.initials: WC
authors.lastname: Shih
mesh_terms: D000208:Acute Disease; D000818:Animals; D000906:Antibodies / Q000276:immunology; D018450:Disease Progression; D005260:Female; D050533:Glomerular Basement Membrane / Q000276:immunology; D008297:Male; D051379:Mice; D015999:Multivariate Analysis; D009393:Nephritis / Q000150:complications / Q000175:diagnosis* / Q000473:pathology; D011507:Proteinuria / Q000150:complications; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D000906:Antibodies
keywords: Raman spectroscopy; acute nephritis; anti-glomerular basement membrane nephritis; linear discriminant analysis; optical diagnosis; principal component analysis