title: Portable gliadin-immunochip for contamination control on the food production chain.
abstract: Celiac disease (CD) is one of the most common digestive disorders caused by an abnormal immune reaction to gluten. So far there are no available therapies, the only solution is a strict gluten-free diet, which however could be very challenging as gluten can be hidden in many food products. Furthermore an additional problem is related to cross-contamination of nominal gluten-free foods with gluten-based ones during manufacturing. Here we propose a lab on chip platform as a powerful tool to help food manufacturers to evaluate the real amount of gluten in their products by an accurate in-situ control of the production chain and maybe to specify the real gluten content in packages labeling. Our portable gliadin-immunochips, based on an electrochemical impedance spectroscopy transduction method, were first calibrated and then validated for both liquid and solid food matrixes by analyzing different beers and flours. The high specificity of our assay was also demonstrated by performing control experiments on rice and potatoes flours containing prolamin-like proteins. We achieved limit of quantification of 0.5 ppm for gliadin that is 20 times lower than the worldwide limit established for gluten-free food while the method of analysis is faster and cheaper than currently employed ELISA-based methods. Moreover our results on food samples were validated through a mass spectrometry standard analysis.
authors.affiliation: NNL Istituto Nanoscienze - CNR and Dipartimento di Matematica e Fisica "Ennio De Giorgi", via per Arnesano, 73100 Lecce, Italy. Electronic address: serena.chiriaco@unisalento.it.
authors.forename: Maria Serena
authors.initials: MS
authors.lastname: Chiriacò
authors.affiliation: NNL Istituto Nanoscienze - CNR and Dipartimento di Matematica e Fisica "Ennio De Giorgi", via per Arnesano, 73100 Lecce, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: de Feo
authors.affiliation: NNL Istituto Nanoscienze - CNR and Dipartimento di Matematica e Fisica "Ennio De Giorgi", via per Arnesano, 73100 Lecce, Italy.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Primiceri
authors.affiliation: NNL Istituto Nanoscienze - CNR and Dipartimento di Matematica e Fisica "Ennio De Giorgi", via per Arnesano, 73100 Lecce, Italy.
authors.forename: Anna Grazia
authors.initials: AG
authors.lastname: Monteduro
authors.affiliation: Laboratorio di spettrometria di massa analitica ed isotopica, Dipartimento di Beni Culturali, Università del Salento, 73100 Lecce, Italy.
authors.forename: Giuseppe Egidio
authors.initials: GE
authors.lastname: de Benedetto
authors.affiliation: Laboratorio di spettrometria di massa analitica ed isotopica, Dipartimento di Beni Culturali, Università del Salento, 73100 Lecce, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Pennetta
authors.affiliation: NNL Istituto Nanoscienze - CNR and Dipartimento di Matematica e Fisica "Ennio De Giorgi", via per Arnesano, 73100 Lecce, Italy.
authors.forename: Ross
authors.initials: R
authors.lastname: Rinaldi
authors.affiliation: NNL Istituto Nanoscienze - CNR and Dipartimento di Matematica e Fisica "Ennio De Giorgi", via per Arnesano, 73100 Lecce, Italy. Electronic address: giuseppe.maruccio@unisalento.it.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Maruccio
mesh_terms: D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D001515:Beer / Q000032:analysis*; D015374:Biosensing Techniques; D058266:Dielectric Spectroscopy; D004566:Electrodes; D005433:Flour / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D005903:Gliadin / Q000032:analysis* / Q000276:immunology; D055767:Immobilized Proteins / Q000737:chemistry / Q000276:immunology; D007118:Immunoassay; D056656:Lab-On-A-Chip Devices; D013058:Mass Spectrometry; D015203:Reproducibility of Results
chemical_list: D000906:Antibodies; D055767:Immobilized Proteins; D005903:Gliadin
keywords: Celiac disease; Electrochemical impedance spectroscopy; Gliadin-Immunochip; Lab on chip; Microfluidics; On-chip contamination control