title: Multipotent stromal cells derived from common marmoset Callithrix jacchus within alginate 3D environment: Effect of cryopreservation procedures.
abstract: Multipotent stromal cells derived from the common marmoset monkey Callithrix jacchus (cjMSCs) possess high phylogenetic similarity to humans, with a great potential for preclinical studies in the field of regenerative medicine. Safe and effective long-term storage of cells is of great significance to clinical and research applications. Encapsulation of such cell types within alginate beads that can mimic an extra-cellular matrix and provide a supportive environment for cells during cryopreservation, has several advantages over freezing of cells in suspension. In this study we have analysed the effect of dimethyl sulfoxide (Me2SO, 2.5-10%, v/v) and pre-freeze loading time of alginate encapsulated cjMSCs in Me2SO (0-45 min) on the viability and metabolic activity of the cells after freezing using a slow cooling rate (-1°C/min). It was found that these parameters affect the stability and homogeneity of alginate beads after thawing. Moreover, the cjMSCs can be frozen in alginate beads with lower Me2SO concentration of 7.5% after 30 min of loading, while retaining high cryopreservation outcome. We demonstrated the maximum viability, membrane integrity and metabolic activity of the cells under optimized, less cytotoxic conditions. The results of this study are another step forward towards the application of cryopreservation for the long-term storage and subsequent applications of transplants in cell-based therapies.
authors.affiliation: Institute for Multiphase Processes, Leibniz Universitaet Hannover, Callinstrasse 36, 30167 Hannover, Germany. Electronic address: gryshkov@imp.uni-hannover.de.
authors.forename: Oleksandr
authors.initials: O
authors.lastname: Gryshkov
authors.affiliation: Institute for Multiphase Processes, Leibniz Universitaet Hannover, Callinstrasse 36, 30167 Hannover, Germany. Electronic address: hofmann@imp.uni-hannover.de.
authors.forename: Nicola
authors.initials: N
authors.lastname: Hofmann
authors.affiliation: Institute for Multiphase Processes, Leibniz Universitaet Hannover, Callinstrasse 36, 30167 Hannover, Germany. Electronic address: lauterboeck@imp.uni-hannover.de.
authors.forename: Lothar
authors.initials: L
authors.lastname: Lauterboeck
authors.affiliation: Institute for Transfusion Medicine, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany. Electronic address: Pogozhykh.Denys@mh-hannover.de.
authors.forename: Denys
authors.initials: D
authors.lastname: Pogozhykh
authors.affiliation: Institute for Transfusion Medicine, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany. Electronic address: mueller.thomas@mh-hannover.de.
authors.forename: Thomas
authors.initials: T
authors.lastname: Mueller
authors.affiliation: Institute for Multiphase Processes, Leibniz Universitaet Hannover, Callinstrasse 36, 30167 Hannover, Germany. Electronic address: glasmacher@imp.uni-hannover.de.
authors.forename: Birgit
authors.initials: B
authors.lastname: Glasmacher
mesh_terms: D000464:Alginates / Q000494:pharmacology; D000818:Animals; D002144:Callithrix / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects; D015925:Cryopreservation / Q000379:methods*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005615:Freezing; D020723:Glucuronic Acid / Q000494:pharmacology; D006603:Hexuronic Acids / Q000494:pharmacology; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D039902:Multipotent Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D010802:Phylogeny
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D004121:Dimethyl Sulfoxide
keywords: Alginate bead; Common marmoset; Cryopreservation; Dimethyl sulfoxide; Encapsulation; High voltage; Ice formation; Multipotent stromal cells; Stem cells; Viability