title: Evaluation of individual sensitivity of head and neck squamous cell carcinoma to cetuximab by short-term culture of tumor slices.
abstract: Cetuximab is a targeted therapy with demonstrated efficacy in the management of head and neck squamous cell carcinoma (HNSCC). However, no laboratory assay is available to predict its efficacy in an individual patient.
  
  
  
  Short-term cultures of tumor slices were performed on 9 tumor samples obtained after surgical resection in patients. Cancer cell proliferation was evaluated by immunohistochemistry and the impact of cetuximab on cell proliferation was examined.
  
  
  
  Tumor architecture and the heterogeneous composition of HNSCC were preserved for at least 48 hours during short-term culture of tumor slices. HNSCC cells demonstrated a heterogeneous individual response to cetuximab.
  
  
  
  Short-term culture of tumor slices is a strategy to estimate the clinical activity of cetuximab in individual patients with HNSCC. Further studies are required to correlate the results obtained with the clinical response of individual patients to cetuximab. © 2015 Wiley Periodicals, Inc. Head Neck 38: E911-E915, 2016.
authors.affiliation: Department of Head and Neck Surgery, University Hospital, Amiens, France.
authors.forename: Marine
authors.initials: M
authors.lastname: Peria
authors.affiliation: Department of Head and Neck Surgery, University Hospital, Amiens, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Donnadieu
authors.affiliation: Department of Maxillofacial Surgery, University Hospital, Amiens, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Racz
authors.affiliation: Department of Pathology, University Hospital, Amiens, France.
authors.forename: Jean-Fortuné
authors.initials: JF
authors.lastname: Ikoli
authors.affiliation: Department of Biochemistry, University Hospital, Amiens, France.|EA 4666, University Picardie-Jules Verne, Amiens, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Galmiche
authors.affiliation: Department of Oncology, University Hospital, Amiens, France.|EA 4666, University Picardie-Jules Verne, Amiens, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Chauffert
authors.affiliation: Department of Head and Neck Surgery, University Hospital, Amiens, France.
authors.forename: Cyril
authors.initials: C
authors.lastname: Page
mesh_terms: D061067:Antibodies, Monoclonal, Humanized; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy*; D049109:Cell Proliferation; D000068818:Cetuximab / Q000627:therapeutic use*; D006258:Head and Neck Neoplasms / Q000188:drug therapy*; D006801:Humans; D014407:Tumor Cells, Cultured
chemical_list: D061067:Antibodies, Monoclonal, Humanized; D000068818:Cetuximab
keywords: cellular culture; cetuximab; head and neck squamous cell carcinoma; head and neck surgery; oral oncology