title: Use of a mannitol rich ensiled grass press juice (EGPJ) as a sole carbon source for polyhydroxyalkanoates (PHAs) production through high cell density cultivation.
abstract: This study demonstrates the use of a mannitol rich ensiled grass press juice (EGPJ) as a renewable carbon substrate for polyhydroxyalkanoates (PHA) production in shaking flask experiments and fed-batch stirred tank reactor cultivations. Fed-batch cultivations of Burkholderia sacchari IPT101 using EGPJ as sole carbon source produced 44.5 g/L CDW containing 33% polyhydroxybutyrate (PHB) in 36 h, while Pseudomonas chlororaphis IMD555 produced a CDW of 37 g/L containing 10% of medium chain length polyhydroxyalkanoates (mcl-PHA) in 34 h. PHB and mcl-PHA extracted from B. sacchari IPT101 and P. chlororaphis IMD555, grown on EGPJ, had a molecular weight of 548 kg/mol and 115.4 kg/mol, respectively. While mcl-PHA can be produced from EGPJ, PHB production is more interesting as there is a 4-fold higher volumetric productivity compared to mcl-PHA.
authors.affiliation: School of Biomolecular and Biomedical Sciences, University College Dublin, Belfield, Dublin 4, Ireland; Earth Institute, University College Dublin, Belfield, Dublin 4, Ireland; Technology Centre for Biorefining and Bioenergy, Orbsen Building, NUIG, Galway, Ireland.
authors.forename: Federico
authors.initials: F
authors.lastname: Cerrone
authors.affiliation: Earth Institute, University College Dublin, Belfield, Dublin 4, Ireland; Technology Centre for Biorefining and Bioenergy, Orbsen Building, NUIG, Galway, Ireland; Centre for Research on Adaptive Nanostructure and Nanodevices, Trinity College Dublin, Dublin 2, Ireland.
authors.forename: Reeta
authors.initials: R
authors.lastname: Davis
authors.affiliation: School of Biomolecular and Biomedical Sciences, University College Dublin, Belfield, Dublin 4, Ireland; Earth Institute, University College Dublin, Belfield, Dublin 4, Ireland.
authors.forename: Shane T
authors.initials: ST
authors.lastname: Kenny
authors.affiliation: Centre for Research on Adaptive Nanostructure and Nanodevices, Trinity College Dublin, Dublin 2, Ireland; School of Physics, Trinity College Dublin, Dublin 2, Ireland.
authors.forename: Trevor
authors.initials: T
authors.lastname: Woods
authors.affiliation: Molecular Glycobiotechnology Group, Biochemistry, School of Natural Sciences, National University of Ireland, Galway, Ireland.
authors.forename: Anthonia
authors.initials: A
authors.lastname: O'Donovan
authors.affiliation: Molecular Glycobiotechnology Group, Biochemistry, School of Natural Sciences, National University of Ireland, Galway, Ireland.
authors.forename: Vijai Kumar
authors.initials: VK
authors.lastname: Gupta
authors.affiliation: Molecular Glycobiotechnology Group, Biochemistry, School of Natural Sciences, National University of Ireland, Galway, Ireland.
authors.forename: Maria
authors.initials: M
authors.lastname: Tuohy
authors.affiliation: Technology Centre for Biorefining and Bioenergy, Orbsen Building, NUIG, Galway, Ireland; Centre for Research on Adaptive Nanostructure and Nanodevices, Trinity College Dublin, Dublin 2, Ireland; School of Physics, Trinity College Dublin, Dublin 2, Ireland.
authors.forename: Ramesh P
authors.initials: RP
authors.lastname: Babu
authors.affiliation: Animal & Grassland Research and Innovation Centre, Teagasc, Grange, Dunsany, Co. Meath, Ireland.
authors.forename: Padraig
authors.initials: P
authors.lastname: O'Kiely
authors.affiliation: School of Biomolecular and Biomedical Sciences, University College Dublin, Belfield, Dublin 4, Ireland; Earth Institute, University College Dublin, Belfield, Dublin 4, Ireland; Technology Centre for Biorefining and Bioenergy, Orbsen Building, NUIG, Galway, Ireland. Electronic address: kevin.oconnor@ucd.ie.
authors.forename: Kevin
authors.initials: K
authors.lastname: O'Connor
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D061249:Batch Cell Culture Techniques / Q000379:methods; D002244:Carbon / Q000378:metabolism*; D002452:Cell Count / Q000379:methods; D008353:Mannitol / Q000378:metabolism*; D006109:Poaceae / Q000378:metabolism*; D054813:Polyhydroxyalkanoates / Q000378:metabolism*
chemical_list: D054813:Polyhydroxyalkanoates; D008353:Mannitol; D002244:Carbon
keywords: Biorefining; Ensiled grass press juice; Mannitol; Polyhydroxyalkanoates; Pulse feed