title: Winogradskyella crassostreae sp. nov., isolated from an oyster (Crassostrea gigas).
abstract: A Gram-stain-negative, non-flagellated, non-gliding, aerobic and rod-shaped bacterium, designated TYO-19(T), was isolated from an oyster collected from the South Sea in South Korea, and was subjected to a taxonomic study using a polyphasic approach. Strain TYO-19(T) grew optimally at 30 °C, at pH 7.0-8.0 and in the presence of 1.0-2.0% (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences showed that strain TYO-19(T) belonged to the genus Winogradskyella, clustering coherently with the type strain of Winogradskyella epiphytica. Strain TYO-19(T) exhibited a 16S rRNA gene sequence similarity value of 99.7% to W. epiphytica KMM 3906(T) and 94.2-96.9% to the type strains of other species of the genus Winogradskyella. Strain TYO-19(T) contained MK-6 as the predominant menaquinone and iso-C15 : 1 G, iso-C17 : 0 3-OH, iso-C15 : 0 and anteiso-C15 : 0 as the major fatty acids. The major polar lipids detected in strain TYO-19(T) were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content was 39.0 mol% and the mean DNA-DNA relatedness value with the type strain of W. epiphytica was 59 ± 4.3%. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strain TYO-19(T) is separated from recognized species of the genus Winogradskyella. On the basis of the data presented, strain TYO-19(T) is considered to represent a novel species of the genus Winogradskyella, for which the name Winogradskyella crassostreae sp. nov. is proposed. The type strain is TYO-19(T) ( = KCTC 42462(T) = NBRC 110924(T)).
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors.forename: Sooyeon
authors.initials: S
authors.lastname: Park
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors.forename: Ji-Min
authors.initials: JM
authors.lastname: Park
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors.forename: Sung-Min
authors.initials: SM
authors.lastname: Won
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D049896:Crassostrea / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C042455:menaquinone 6