title: Retrieving nitrogen isotopic signatures from fresh leaf reflectance spectra: disentangling δ(15)N from biochemical and structural leaf properties.
abstract: Linking remote sensing methodology to stable isotope ecology provides a promising approach to study ecological processes from small to large spatial scales. Here, we show that δ(15)N can be detected in fresh leaf reflectance spectra of field samples along a spatial gradient of increasing nitrogen input from an N2-fixing invasive species. However, in field data it is unclear whether δ(15)N directly influences leaf reflectance spectra or if the relationship is based on covariation between δ(15)N and foliar nitrogen content or other leaf properties. Using a (15)N-labeling approach, we experimentally varied δ(15)N independently of any other leaf properties in three plant species across different leaf developmental and physiological states. δ(15)N could successfully be modeled by means of partial least squares (PLSs) regressions, using leaf reflectance spectra as predictor variables. PLS models explained 53-73% of the variation in δ(15)N within species. Several wavelength regions important for predicting δ(15)N were consistent across species and could furthermore be related to known absorption features of N-containing molecular bonds. By eliminating covariation with other leaf properties as an explanation for the relationship between reflectance and δ(15)N, our results demonstrate that (15)N itself has an inherent effect on leaf reflectance spectra. Thus, our study substantiates the use of spectroscopic measurements to retrieve isotopic signatures for ecological studies and encourages future development. Furthermore, our results highlight the great potential of optical measurements for up-scaling isotope ecology to larger spatial scales.
authors.affiliation: Experimental and Systems Ecology, University of Bielefeld, Bielefeld Germany ; AgroEcosystem Research, Bayreuth Center of Ecology and Ecosystem Research, University of Bayreuth, Bayreuth Germany.
authors.forename: Christine
authors.initials: C
authors.lastname: Hellmann
authors.affiliation: Institute of Landscape Ecology, University of Münster, Münster Germany.
authors.forename: André
authors.initials: A
authors.lastname: Große-Stoltenberg
authors.affiliation: Experimental and Systems Ecology, University of Bielefeld, Bielefeld Germany ; Plant Ecology and Conservation, Institute of Botany, Geisenheim University, Geisenheim Germany.
authors.forename: Verena
authors.initials: V
authors.lastname: Lauströ
authors.affiliation: Biodiversity, Evolution and Ecology of Plants, Biocentre Klein Flottbek and Botanical Garden, University of Hamburg, Hamburg Germany.
authors.forename: Jens
authors.initials: J
authors.lastname: Oldeland
authors.affiliation: AgroEcosystem Research, Bayreuth Center of Ecology and Ecosystem Research, University of Bayreuth, Bayreuth Germany.
authors.forename: Christiane
authors.initials: C
authors.lastname: Werner
keywords: PLS; labeling; leaf reflectance spectra; spatial scale; spectroscopy; stable isotopes; δ15N