title: Hepatoprotective activity of hepatoplus on isonaizid and rifampicin induced hepatotoxicity in rats.
abstract: Present study deals with the hepatoprotective activity of polyherbal formulation Hepatoplus (HP) as an oral supplement to the INH and RIF induced hepatitis in experimental rats. Rats treated with INH and RIF show abnormal liver function with significant increase in serum transaminases, bilirubin and clotting time (CT) and significant decrease in total protein and Albumin, which is brings to near normal levels by HP and LIV 52 treatments. Rats treated with INH and RIF suffer from oxidative stress in the hepatocytes, due to the decrease in Glutathione (GSH), Glutathione peroxidase (GPX), Catalase (CAT), Super oxide dismutase (SOD) and significant increase in Lipid Per oxidation (LPO). HP decreases the oxidative stress and protects the liver cells membrane from LPO. 85% of DNA damage (comet tail) seen with RIF and INH treatment is reduced to 34.1% on HP application. A decrease of hepatocytes mitochondrial dehydrogenase activity is observed in INH and RIF treatment is restored by HP supplementation. Hepatic apoptotic and CYP2E1 gene expressions were also studied, BAX, p53, Caspase 3 and CYP2E1 were significantly up regulated and Bcl2 was down- regulated in INH and RIF treated rats. Concomitant application of HP prevents the modulation of these gene expressions. It is concluded that high dose of HP (100mg/kg) supplemented along with INH and RIF effectively prevents the toxicity induced by INH and RIF, as effective as 100mg/kg of LIV52.
authors.affiliation: Department of Biotechnology, Rajalakshmi Engineering College, Chennai, India.
authors.forename: Madhavan
authors.initials: M
authors.lastname: Sankar
authors.affiliation: Department of Biotechnology, Rajalakshmi Engineering College, Chennai, India.
authors.forename: Johanna
authors.initials: J
authors.lastname: Rajkumar
authors.affiliation: Department of Biotechnology, Rajalakshmi Engineering College, Chennai, India.
authors.forename: Jamuna
authors.initials: J
authors.lastname: Devi
mesh_terms: D000818:Animals; D053148:Caspase 3 / Q000187:drug effects / Q000235:genetics; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology / Q000235:genetics* / Q000378:metabolism; D030024:Curcuma; D029784:Cycas; D019392:Cytochrome P-450 CYP2E1 / Q000187:drug effects / Q000235:genetics; D036601:Eclipta; D015870:Gene Expression / Q000187:drug effects*; D016158:Genes, p53 / Q000187:drug effects; D007538:Isoniazid / Q000633:toxicity*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008515:Medicine, Ayurvedic; D029595:Orchidaceae; D029803:Phyllanthus; D032261:Picrorhiza; D028223:Pinus; D027927:Pistacia; D028321:Plant Preparations / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D012293:Rifampin / Q000633:toxicity*; D031304:Tephrosia; D032341:Withania; D051028:bcl-2-Associated X Protein / Q000187:drug effects / Q000235:genetics
chemical_list: C494828:Bax protein, rat; D028321:Plant Preparations; D020011:Protective Agents; D051028:bcl-2-Associated X Protein; D019392:Cytochrome P-450 CYP2E1; C505410:Casp3 protein, rat; D053148:Caspase 3; D007538:Isoniazid; D012293:Rifampin