title: Layer-resolved colorectal tissues using nonlinear microscopy.
abstract: In this work, multiphoton microscopy (MPM), based on the nonlinear optical processes two-photon excited fluorescence (TPEF) and second harmonic generation (SHG), was extended to evaluate the feasibility of using MPM to distinguish layers of the bowel wall. It was found that MPM has the ability to identify the four-layer microstructures of colorectal tissues including mucosa, submucosa, muscularis propria, and serosa as there are many intrinsic signal sources in each layer. Our results also showed the capability of using the quantitative analyses of MPM images for quantifying some feature parameters including the nuclear area, nuclear-to-cytoplasmic ratio, and optical redox ratio. This work demonstrates that MPM has the potential in noninvasively monitoring the development and progression of colorectal diseases and then guiding effective treatment.
authors.affiliation: Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, 350007, China.
authors.forename: Lianhuang
authors.initials: L
authors.lastname: Li
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Li
authors.forename: Zhifen
authors.initials: Z
authors.lastname: Chen
authors.forename: Shuangmu
authors.initials: S
authors.lastname: Zhuo
authors.forename: Changyin
authors.initials: C
authors.lastname: Feng
authors.forename: Yinghong
authors.initials: Y
authors.lastname: Yang
authors.forename: Guoxian
authors.initials: G
authors.lastname: Guan
authors.forename: Jianxin
authors.initials: J
authors.lastname: Chen
mesh_terms: D003106:Colon / Q000473:pathology*; D015179:Colorectal Neoplasms / Q000175:diagnosis; D006801:Humans; D007413:Intestinal Mucosa / Q000473:pathology; D036641:Microscopy, Fluorescence, Multiphoton; D012007:Rectum / Q000473:pathology*