title: MicroRNA-130a Targets MAP3K12 to Modulate Diabetic Endothelial Progenitor Cell Function.
abstract: OBJECTIVE
  The aim of the present study was to explore the influence of microRNA (miR)-130a dysregulation on the JNK signal pathway through its target MAP3K12 in diabetic endothelial progenitor cells (EPCs).
  
  
  METHODS
  The expression of miR-130a was compared between diabetic and normal EPCs. Computational target prediction was performed to identify MAP3K12 as a functionally relevant target of miR-130a in EPCs. The role of miR-130a was investigated regarding its anti-apoptotic effects and its role on the regulation of EPC function was evaluated through the negative regulation of the JNK signal pathway
  
  
  RESULTS
  MiR-130a expression was significantly downregulated in diabetic EPCs, and cell proliferation was reduced in EPCs under high glucose condition. miR-130a inhibited the JNK pathway by targeting MAP3K12, contributing to its anti-apoptotic effect and the maintenance of EPC function. In diabetic EPCs, high glucose affects the expression of miR-130a, inducing sustained JNK activation and promoting EPC apoptosis and dysfunction.
  
  
  CONCLUSIONS
  Downregulation of miR-130a may underlie endothelial dysfunction in diabetes through the activation of JNK signal pathway.
authors.forename: Meng
authors.initials: M
authors.lastname: Ye
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
authors.forename: Jing
authors.initials: J
authors.lastname: Xie
authors.forename: Fei
authors.initials: F
authors.lastname: Yu
authors.forename: Yushui
authors.initials: Y
authors.lastname: Ma
authors.forename: Xuchao
authors.initials: X
authors.lastname: Zhu
authors.forename: Jinwei
authors.initials: J
authors.lastname: Zhao
authors.forename: Zhongwei
authors.initials: Z
authors.lastname: Lv
mesh_terms: D000368:Aged; D017209:Apoptosis; D049109:Cell Proliferation; D002478:Cells, Cultured; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D015536:Down-Regulation; D066026:Endothelial Progenitor Cells / Q000166:cytology / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D020930:MAP Kinase Kinase Kinases / Q000235:genetics* / Q000378:metabolism; D020935:MAP Kinase Signaling System; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D015854:Up-Regulation
chemical_list: C554019:MIRN130 microRNA, human; D035683:MicroRNAs; D020930:MAP Kinase Kinase Kinases; C090757:mitogen-activated protein kinase kinase kinase 12