title: Biosurfactant produced from Actinomycetes nocardiopsis A17: Characterization and its biological evaluation.
abstract: This investigation aims to isolate an Actinomycetes strain producing a biosurfactant from the unexplored region of industrial and coal mine areas. Actinomycetes are selected for this study as their novel chemistry was not exhausted and they have tremendous potential to produce bioactive secondary metabolites. The biosurfactant was characterized and further needed to be utilized for pharmaceutical dosage form. Isolation, purification, screening, and characterization of the Actinomycetes A17 were done followed by its fermentation in optimized conditions. The cell-free supernatant was used for the extraction of the biosurfactant and precipitated by cold acetone. The dried precipitate was purified by TLC and the emulsification index, surface tension and CMC were determined. The isolated strain with preferred results was identified as Actinomycetes nocardiopsis A17 with high foam-forming properties. It gives lipase, amylase, gelatinase, and protease activity. The emulsification index was found to be 93±0.8 with surface tension 66.67 dyne/cm at the lowest concentration and cmc 0.6 μg/ml. These biosurfactants were characterized by Fourier transform infra red (FT-IR) spectroscopy and liquid chromatography-mass spectrometry (LC-MS). Therefore, it can be concluded that the biosurfactant produced by Actinomycetes nocardiopsis sp. strain A17 was found to have satisfactory results with high surface activity and emulsion-forming ability.
authors.affiliation: Department of Pharmaceutics, Gupta College of Technological Sciences, Asansol 713301, WB, India.
authors.forename: Samrat
authors.initials: S
authors.lastname: Chakraborty
authors.affiliation: Department of Pharmaceutics, Gupta College of Technological Sciences, Asansol 713301, WB, India.
authors.forename: Mandakini
authors.initials: M
authors.lastname: Ghosh
authors.affiliation: Department of Pharmaceutics, Gupta College of Technological Sciences, Asansol 713301, WB, India.
authors.forename: Srijita
authors.initials: S
authors.lastname: Chakraborti
authors.affiliation: Department of Pharmaceutics, Gupta College of Technological Sciences, Asansol 713301, WB, India. Electronic address: janapharmacy@rediffmail.com.
authors.forename: Sougata
authors.initials: S
authors.lastname: Jana
authors.affiliation: Department of Pharmaceutics, Gupta College of Technological Sciences, Asansol 713301, WB, India.
authors.forename: Kalyan Kumar
authors.initials: KK
authors.lastname: Sen
authors.affiliation: Department of Pharmaceutics, Sinhgad Institute of Pharmacy, Narhe, Pune 411 041, India.
authors.forename: Chandrakant
authors.initials: C
authors.lastname: Kokare
authors.affiliation: Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Lixin
authors.initials: L
authors.lastname: Zhang
mesh_terms: D039903:Actinobacteria / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D000681:Amylases / Q000096:biosynthesis / Q000302:isolation & purification; D004655:Emulsions; D005285:Fermentation; D018093:Gelatinases / Q000096:biosynthesis / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000096:biosynthesis / Q000302:isolation & purification; D008823:Micelles; D010447:Peptide Hydrolases / Q000096:biosynthesis / Q000302:isolation & purification; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D013500:Surface Tension; D013501:Surface-Active Agents / Q000302:isolation & purification / Q000378:metabolism*; D013696:Temperature
chemical_list: D004655:Emulsions; D008823:Micelles; D012336:RNA, Ribosomal, 16S; D013501:Surface-Active Agents; D008049:Lipase; D000681:Amylases; D010447:Peptide Hydrolases; D018093:Gelatinases
keywords: Actinomycetes A17; Biosurfactant; Critical micelle concentration (CMC)