title: Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections.
abstract: An increasing amount of research has been conducted on immunoglobulin Y (IgY) because the use of IgY offers several advantages with respect to diagnostic testing, including its easy accessibility, low cost and translatability to large-scale production, in addition to the fact that it can be ethically produced. In a previous work, immunoglobulin was produced and purified from egg yolks (IgY) reactive to hepatitis A virus (HAV) antigens. In the present work, this anti-HAV-specific IgY was used in an indirect immunofluorescence assay to detect viral antigens in liver biopsies that were obtained from experimentally infected cynomolgus monkeys. Fields that were positive for HAV antigen were detected in liver sections using confocal microscopy. In conclusion, egg yolks from immunised hens may be a reliable source for antibody production, which can be employed for immunological studies.
authors.affiliation: Laboratório de Desenvolvimento Tecnológico em Virologia, Instituto Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Gentil Arthur
authors.initials: GA
authors.lastname: Bentes
authors.affiliation: Laboratório de Desenvolvimento Tecnológico em Virologia, Instituto Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Natália Maria
authors.initials: NM
authors.lastname: Lanzarini
authors.affiliation: Laboratório de Desenvolvimento Tecnológico em Virologia, Instituto Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Lyana Rodrigues Pinto
authors.initials: LR
authors.lastname: Lima
authors.affiliation: Laboratório de Patologia, Instituto Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Pedro Paulo de Abreu
authors.initials: PP
authors.lastname: Manso
authors.affiliation: Laboratório de Desenvolvimento Tecnológico em Virologia, Instituto Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Alexandre Dos Santos
authors.initials: Ados S
authors.lastname: da Silva
authors.affiliation: Laboratório de Virologia Comparada e Ambiental, Instituto Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Sergio da Silva E
authors.initials: Sda S
authors.lastname: Mouta Junior
authors.affiliation: Laboratório de Desenvolvimento Tecnológico em Virologia, Instituto Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Juliana Rodrigues
authors.initials: JR
authors.lastname: Guimarães
authors.affiliation: Laboratório de Virologia Comparada e Ambiental, Instituto Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Marcia Terezinha Baroni
authors.initials: MT
authors.lastname: de Moraes
authors.affiliation: Laboratório de Patologia, Instituto Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Pelajo-Machado
authors.affiliation: Laboratório de Desenvolvimento Tecnológico em Virologia, Instituto Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Marcelo Alves
authors.initials: MA
authors.lastname: Pinto
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D019084:Fluorescent Antibody Technique, Indirect; D006506:Hepatitis A / Q000175:diagnosis* / Q000276:immunology; D035922:Hepatitis A Antibodies / Q000276:immunology; D035921:Hepatitis A Antigens / Q000276:immunology; D030041:Hepatitis A virus / Q000276:immunology*; D007136:Immunoglobulins / Q000032:analysis*; D008099:Liver / Q000821:virology*; D008252:Macaca fascicularis; D012680:Sensitivity and Specificity
chemical_list: D035922:Hepatitis A Antibodies; D035921:Hepatitis A Antigens; C016512:IgY; D007136:Immunoglobulins