title: Study of molecule variations in human xanthelasma skin based on confocal micro-raman spectroscopy.
abstract: Confocal micro-Raman spectroscopy was used to distinguish human xanthelasma skin (HXS) from the human normal skin (HNS). Results showed that intensive Raman peaks at 1,269, 1,336, 1,448, and 1,656 cm(-1) increased obviously. Both 1,269 and 1,656 cm(-1) peaks showed that the proteins in HXS were mostly in the anti-parallel ß sheet conformation. While the intensities of bands at 1,032, 1,087, 1,300, and 1,448 cm(-1) belonged to lipids were enhanced in HXS spectrum compared to those in HNS spectrum. There were main intercellular lipids alkyl chains with minor proteins contribution at 1,087 cm(-1) and phenylalanine at 1,032 cm(-1) . To quantitative analysis of the difference, the ratio of I852/I829 was calculated, which was 1:1.04 ± 0.04 and 1:1.11 ± 0.02 for HNS and HXS (p < 0.01), respectively. The data indicated that some tyrosine residues, which form a hydrogen bond with H2 O prior to aggregation, were captured by strong hydrogen-bond acceptors in the aggregate. The decreased ratio of I852/I829 indicated more hydrophobic in HXS than HNS. Principal component analysis showed a one-to-one relationship between human xanthelasma skin and the corresponding Raman spectra. It could be given useful help for the diagnostication of xanthelasma.
authors.affiliation: MOE Key Laboratory of Laser Life Science & Laboratory of Photonic Chinese Medicine, College of Biophotonics, South China Normal University, Guangzhou, China.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Qiu
authors.affiliation: Department of Ophthalmology, The Third Affiliated Hospital of Southern Medical University, Guangzhou, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Xiong
authors.affiliation: National Testing Center for Optical Radiation Safety of Photoelectric Products, Huizhou, China.
authors.forename: Xiangping
authors.initials: X
authors.lastname: Ye
authors.affiliation: Guzhen Productivity Promotion Center, Zhongshan, China.
authors.forename: Zhitong
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Department of Biological Engineering, Changsha University, Changsha, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: MOE Key Laboratory of Laser Life Science & Laboratory of Photonic Chinese Medicine, College of Biophotonics, South China Normal University, Guangzhou, China.
authors.forename: Chengkang
authors.initials: C
authors.lastname: Su
authors.affiliation: MOE Key Laboratory of Laser Life Science & Laboratory of Photonic Chinese Medicine, College of Biophotonics, South China Normal University, Guangzhou, China.
authors.forename: Zhengfei
authors.initials: Z
authors.lastname: Zhuang
authors.affiliation: MOE Key Laboratory of Laser Life Science & Laboratory of Photonic Chinese Medicine, College of Biophotonics, South China Normal University, Guangzhou, China.
authors.forename: Zhouyi
authors.initials: Z
authors.lastname: Guo
authors.affiliation: MOE Key Laboratory of Laser Life Science & Laboratory of Photonic Chinese Medicine, College of Biophotonics, South China Normal University, Guangzhou, China.
authors.forename: Songhao
authors.initials: S
authors.lastname: Liu
mesh_terms: D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012867:Skin / Q000737:chemistry* / Q000473:pathology*; D012871:Skin Diseases / Q000473:pathology*; D013059:Spectrum Analysis, Raman / Q000379:methods*
keywords: Raman spectroscopy; lipids; molecule variation; xanthelasma tissue