title: Performance of lateral flow device and galactomannan for the detection of Aspergillus species in bronchoalveolar fluid of patients at risk for invasive pulmonary aspergillosis.
abstract: Early diagnosis of invasive pulmonary aspergillosis (IPA) remains difficult due to the variable performance of the tests used. We compared the performance characteristics of Aspergillus lateral flow device (LFD) in bronchoalveolar lavage (BAL) vs. BAL-galactomannan (GM), for the diagnosis of IPA. 311 BAL specimens were prospectively collected from patients who underwent bronchoscopy from January to May 2013. Patients at risk for IPA were divided into haematological malignancy (HEM) and non-HEM groups: solid organ transplants (SOT) (lung transplant (LT) and non-LT SOT); chronic steroid use (CSU); solid tumour (STU) and others. We identified 96 patients at risk for IPA; 89 patients (93%) were in the non-HEM groups: SOT 57 (LT, 46, non-LT SOT, 11); CSU 21; STU 6, other 5. Only three patients met criteria for IA (two probable; one possible). Overall sensitivity (SS) was 66% for both and specificity (SP) was 94% vs. 52% for LFD and GM respectively. LFD and GM performance was similar in the HEM group (SS 100% for both and SP 83% vs. 100% respectively). LFD performance was better than GM among non-HEM SOT patients (P = 0.02). Most false-positive GM results occurred in the SOT group (50.8%), especially among LT patients (56.5%). LFD performance was superior with an overall SP of 95.6% in SOT (P < 0.002) and 97% in LT patients (P = 0.0008). LFD is a rapid and simple test that can be performed on BAL to rule out IPA.
authors.affiliation: Division of Infectious Diseases, Department of Internal Medicine, University of Michigan, Ann Arbor, MI, USA.
authors.forename: Marisa H
authors.initials: MH
authors.lastname: Miceli
authors.affiliation: Division of Infectious Diseases, Department of Internal Medicine, Henry Ford Health System, Detroit, MI, USA.
authors.forename: Michael I
authors.initials: MI
authors.lastname: Goggins
authors.affiliation: Division of Infectious Diseases, Department of Internal Medicine, Henry Ford Health System, Detroit, MI, USA.
authors.forename: Pranay
authors.initials: P
authors.lastname: Chander
authors.affiliation: Division of Infectious Diseases, Department of Internal Medicine, Henry Ford Health System, Detroit, MI, USA.
authors.forename: Archana K
authors.initials: AK
authors.lastname: Sekaran
authors.affiliation: Division of Infectious Diseases, Department of Internal Medicine, Henry Ford Health System, Detroit, MI, USA.
authors.forename: Anne E
authors.initials: AE
authors.lastname: Kizy
authors.affiliation: Department of Microbiology and Pathology, Henry Ford Health System, Detroit, MI, USA.
authors.forename: Linoj
authors.initials: L
authors.lastname: Samuel
authors.affiliation: Department of Biostatistics, University of Michigan, Ann Arbor, MI, USA.
authors.forename: Hui
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Biosciences, College of Life and Environmental Sciences, University of Exeter, Exeter, UK.
authors.forename: Christopher R
authors.initials: CR
authors.lastname: Thornton
authors.affiliation: Division of Infectious Diseases, Department of Internal Medicine, Henry Ford Health System, Detroit, MI, USA.
authors.forename: Mayur
authors.initials: M
authors.lastname: Ramesh
authors.affiliation: Division of Infectious Diseases, Department of Internal Medicine, Henry Ford Health System, Detroit, MI, USA.
authors.forename: George
authors.initials: G
authors.lastname: Alangaden
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000946:Antigens, Fungal / Q000032:analysis*; D001230:Aspergillus / Q000737:chemistry*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D005189:False Positive Reactions; D005260:Female; D005690:Galactose / Q000031:analogs & derivatives; D006801:Humans; D055744:Invasive Pulmonary Aspergillosis / Q000175:diagnosis*; D008297:Male; D008351:Mannans / Q000032:analysis*; D008875:Middle Aged; D011446:Prospective Studies; D012680:Sensitivity and Specificity
chemical_list: D000946:Antigens, Fungal; D008351:Mannans; C012990:galactomannan; D005690:Galactose
keywords: Aspergillus; brochoalveolar lavage; diagnosis; lateral flow device