title: The influence of budA deletion on glucose metabolism related in 2,3-butanediol production by Klebsiella pneumoniae.
abstract: Klebsiella pneumoniae (K. pneumoniae), which is a promising microorganism for industrial bulk production of 2,3-butanediol (2,3-BDO), naturally converts glucose to 2,3-BDO. The 2,3-BDO biosynthesis from glucose is composed of three steps; α-acetolactate biosynthesis by α-acetolactate synthase (budB); acetoin biosynthesis by α-acetolactate decarboxylase (budA); and 2,3-BDO biosynthesis by acetoin reductase (budC). In an effort to understand the influence of blocked 2,3-BDO pathway on K. pneumoniae glucose metabolism by budA deletion, we constructed K. pneumoniaeΔwabGΔbudA (SGSB106). Carbon flux distribution analysis, transcriptome analysis and extracellular amino acid concentration analysis were carried out to understand the effects of the budA deletion, and K. pneumoniaeΔwabG (SGSB100) was used as a control strain. Approximately 50.3% decrease in CO2 emission; and approximately 3.8-fold increase in amino acid production was observed in SGSB106. In addition to, among the amino acids, valine production significantly increased, suggesting that the branched-chain amino acid biosynthesis (BACC) in SGSB106 was activated by deletion of budA. Furthermore, whole genome transcriptome analysis of SGSB106 and SGSB100, correlates with the results from carbon distribution and amino acids concentration analyses.
authors.affiliation: Department of Chemical and Biomolecular Engineering, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul 121-742, South Korea.
authors.forename: Borim
authors.initials: B
authors.lastname: Kim
authors.affiliation: Department of Chemical and Biomolecular Engineering, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul 121-742, South Korea.
authors.forename: Soojin
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Chemical and Biomolecular Engineering, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul 121-742, South Korea.
authors.forename: Jeongmo
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Chemical and Biomolecular Engineering, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul 121-742, South Korea.
authors.forename: Daun
authors.initials: D
authors.lastname: Jeong
authors.affiliation: Macrogen Inc., 254 Beotkkot-ro, Geumcheon-gu, Seoul, South Korea.
authors.forename: Sang Heum
authors.initials: SH
authors.lastname: Shin
authors.affiliation: Macrogen Inc., 254 Beotkkot-ro, Geumcheon-gu, Seoul, South Korea.
authors.forename: Jun Ho
authors.initials: JH
authors.lastname: Kook
authors.affiliation: Macrogen Inc., 254 Beotkkot-ro, Geumcheon-gu, Seoul, South Korea.
authors.forename: Kap-Seok
authors.initials: KS
authors.lastname: Yang
authors.affiliation: Department of Chemical and Biomolecular Engineering, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul 121-742, South Korea. Electronic address: jinwonlee@sogang.ac.kr.
authors.forename: Jinwon
authors.initials: J
authors.lastname: Lee
mesh_terms: D000596:Amino Acids / Q000096:biosynthesis*; D000597:Amino Acids, Branched-Chain / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002072:Butylene Glycols / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D002262:Carboxy-Lyases; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005947:Glucose / Q000378:metabolism*; D007711:Klebsiella pneumoniae / Q000235:genetics* / Q000378:metabolism; D007773:Lactates / Q000378:metabolism
chemical_list: D000596:Amino Acids; D000597:Amino Acids, Branched-Chain; D001426:Bacterial Proteins; D002072:Butylene Glycols; D007773:Lactates; D002245:Carbon Dioxide; C026978:2,3-butylene glycol; C006359:alpha-acetolactate; D002262:Carboxy-Lyases; C023746:acetolactate decarboxylase; D005947:Glucose
keywords: 2 ;3-butanediol; Amino acid analysis; Carbon distribution analysis; Metabolome analysis; Transcriptome analysis; α-acetolactate decarboxylase