title: Glyco-Nanoparticles Made from Self-Assembly of Maltoheptaose-block-Poly(methyl methacrylate): Micelle, Reverse Micelle, and Encapsulation.
abstract: The synthesis and the solution-state self-assembly of the "hybrid" diblock copolymers, maltoheptaose-block-poly(methyl methacrylate) (MH-b-PMMA), into large compound micelles (LCMs) and reverve micelle-type nanoparticles, are reported in this paper. The copolymers were self-assembled in water and acetone by direct dissolution method, and the morphologies of the nanoparticles were investigated by dynamic light scattering (DLS), nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), atomic force microscopy (AFM), proton nuclear magnetic resonance ((1)H NMR), and fluorescence spectroscopy as a function of the volume fraction of the copolymer hydrophobic block, copolymer concentration, stirring speed, and solvent polarity. The DLS measurements and TEM images showed that the hydrodynamic radius (Rh) of the LCMs obtained in water increases with the copolymer concentration. Apart from that, increasing the stirring speed leads to polydispersed aggregations of the LCMs. On the other hand, in acetone, the copolymers self-assembled into reverse micelle-type nanoparticles having Rh values of about 6 nm and micellar aggregates, as revealed the results obtained from DLS, AFM, and (1)H NMR analyses. The variation in micellar structure, that is, conformational inversion from LCMs to reverse micelle-type structures in response to polarity of the solvent, was investigated by apparent water contact angle (WCA) and (1)H NMR analyses. This conformational inversion of the nanoparticles was further confirmed by encapsulation and release of hydrophobic guest molecule, Nile red, characterized by fluorescence spectroscopy.
authors.affiliation: †University Grenoble Alpes, CERMAV, F-38000 Grenoble, France.|‡CNRS, CERMAV, F-38000 Grenoble, Cedex 9, France.|§Departamento de Química, Universidade Federal de Santa Catarina, CEP 88040-900, Florianópolis, Santa Catarina, Brazil.
authors.forename: Karine M
authors.initials: KM
authors.lastname: Zepon
authors.affiliation: †University Grenoble Alpes, CERMAV, F-38000 Grenoble, France.|‡CNRS, CERMAV, F-38000 Grenoble, Cedex 9, France.
authors.forename: Issei
authors.initials: I
authors.lastname: Otsuka
authors.affiliation: ∥Institut Charles Gerhardt Montpellier UMR5253 CNRS-UM-ENSCM, Equipe Ingénierie et Architectures Macromoléculaires, Université Montpellier, Bâtiment 17-cc1702, Place Eugène Bataillon, 34095 Montpellier, Cedex 5, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Bouilhac
authors.affiliation: ⊥Grupo de Materiais Poliméricos e Compósitos, GMPC-Departamento de Química, Universidade Estadual de Maringá, CEP 87020-900, Maringá, Paraná, Brazil.
authors.forename: Edvani C
authors.initials: EC
authors.lastname: Muniz
authors.affiliation: §Departamento de Química, Universidade Federal de Santa Catarina, CEP 88040-900, Florianópolis, Santa Catarina, Brazil.
authors.forename: Valdir
authors.initials: V
authors.lastname: Soldi
authors.affiliation: †University Grenoble Alpes, CERMAV, F-38000 Grenoble, France.|‡CNRS, CERMAV, F-38000 Grenoble, Cedex 9, France.
authors.forename: Redouane
authors.initials: R
authors.lastname: Borsali
mesh_terms: D000067493:Dynamic Light Scattering; D005936:Glucans / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008823:Micelles; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D014867:Water
chemical_list: D005936:Glucans; D008823:Micelles; D014867:Water; C031146:maltoheptaose; D011092:Polyethylene Glycols; D019904:Polymethyl Methacrylate