title: MicroRNA-19a regulates lipopolysaccharide-induced endothelial cell apoptosis through modulation of apoptosis signal-regulating kinase 1 expression.
abstract: BACKGROUND
  MicroRNAs, small non-encoding RNAs that post-transcriptionally modulate expression of their target genes, have been implicated as critical regulatory molecules in endothelial cells.
  
  
  RESULTS
  In the present study, we found that overexpression of miR-19a protects endothelial cells from lipopolysaccharide (LPS)-induced apoptosis through the apoptosis signal-regulating kinase 1 (ASK1)/p38 pathway. Quantitative real-time PCR demonstrated that the expression of miR-19a in endothelial cell was markedly down-regulated by LPS stimulation. Furthermore, LPS-induced apoptosis was significantly inhibited by over-expression of miR-19a. Finally, both a luciferase reporter assay and western blot analysis showed that ASK1 is a direct target of miR-19a.
  
  
  CONCLUSIONS
  MiR-19a regulates ASK1 expression by targeting specific binding sites in the 3' untranslated region of ASK1 mRNA. Overexpression of miR-19a is an effective method to protect against LPS-induced apoptosis of endothelial cells.
authors.affiliation: Department of Respiration, Jiangyin Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Jiangyin City, Jiangsu Province, 214400, China. jwljytcm@163.com.
authors.forename: Wei-Long
authors.initials: WL
authors.lastname: Jiang
authors.affiliation: Department of Respiration, Jiangyin Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Jiangyin City, Jiangsu Province, 214400, China. zyfjytcm@163.com.
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Zhang
authors.affiliation: Department of Respiration, Jiangyin Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Jiangyin City, Jiangsu Province, 214400, China. xqqjytcm@163.com.
authors.forename: Qing-Qing
authors.initials: QQ
authors.lastname: Xia
authors.affiliation: Department of Neurology, Jiangyin Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Jiangyin City, Jiangsu Province, 214400, China. zjjytcm@163.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Internal Medicine, Jiangyin Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Jiangyin City, Jiangsu Province, 214400, China. yxjytcm@163.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of Neurology, Jiangyin Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Jiangyin City, Jiangsu Province, 214400, China. ftjytcm@163.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Fan
authors.affiliation: Department of Neurology, Shanghai First People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200080, China. wfwangfeng@yahoo.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
mesh_terms: D017209:Apoptosis; D001665:Binding Sites; D002460:Cell Line; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005786:Gene Expression Regulation; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology*; D048848:MAP Kinase Kinase Kinase 5 / Q000737:chemistry / Q000235:genetics*; D020935:MAP Kinase Signaling System / Q000187:drug effects; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics
chemical_list: D008070:Lipopolysaccharides; C549214:MIRN19A microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D048848:MAP Kinase Kinase Kinase 5; C482858:MAP3K5 protein, human