title: Association study of genetic variants at newly identified lipid gene TRIB1 with coronary heart disease in Chinese Han population.
abstract: BACKGROUND
  Recent genome-wide association studies (GWAS) have identified the variants near TRIB1 gene affecting blood lipid levels. However, the association between the reported variants and risk of coronary heart disease (CHD) was not confirmed.
  
  
  METHODS
  We conducted two independent case-control studies. The first study consisted of 300 CHD patients and 300 controls and the second study had 1,332 CHD patients and 2,811 controls. The genotypes of two variants rs3201475 and rs17321515 in TRIB1 were determined by TaqMan assay. The dual-luciferase reporter assay was performed for evaluating the function of the SNP rs3201475.
  
  
  RESULTS
  The statistical analysis indicated that single nucleotide polymorphism (SNP) rs17321515 was replicated to be associated with triglyceride (TG) level, which was also significantly associated with CHD risk when using the stratified analysis after adjusting for conventional risk factors. Compared with GG genotype, AA carriers of SNP rs17321515 had higher risk in males (odds ratio (OR)=1.28, 95%CI=1.01-1.61; P=0.03) and smokers (OR=1.41, 95%CI=1.09-1.88; P=0.01). We did not find significantly association between genotypes of rs3201475 and CHD risk. In addition, no significant difference was found in the luciferase activity assay of SNP rs3201475.
  
  
  CONCLUSIONS
  Our findings indicated that SNP rs17321515 is significantly associated with plasma TG level and the increasing risk of CHD among males and smokers in Chinese, whereas there is no positive association between SNP rs3201475 and CHD risk. Smoking could modify the effects of TRIB1 on CHD risk.
authors.affiliation: Department of Epidemiology, the Innovation Center for Social Risk Governance in Health, School of Public Health and Management, Chongqing Medical University, Chongqing, 400016, China. 616786539@qq.com.
authors.forename: Long
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Cardiology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010, China. 553072770@qq.com.
authors.forename: Jinjin
authors.initials: J
authors.lastname: Jing
authors.affiliation: Department of Epidemiology, the Innovation Center for Social Risk Governance in Health, School of Public Health and Management, Chongqing Medical University, Chongqing, 400016, China. 598812520@qq.com.
authors.forename: Qianxi
authors.initials: Q
authors.lastname: Fu
authors.affiliation: Department of Epidemiology, the Innovation Center for Social Risk Governance in Health, School of Public Health and Management, Chongqing Medical University, Chongqing, 400016, China. 362054677@qq.com.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Tang
authors.affiliation: Department of Cardiology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010, China. 12850031@qq.com.
authors.forename: Li
authors.initials: L
authors.lastname: Su
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health and Management, Chongqing Medical University, Chongqing, 400016, China. 984975447@qq.com.
authors.forename: Shishi
authors.initials: S
authors.lastname: Wu
authors.affiliation: Department of Epidemiology, the Innovation Center for Social Risk Governance in Health, School of Public Health and Management, Chongqing Medical University, Chongqing, 400016, China. lige@vip.sina.com.|Department of Epidemiology, School of Public Health and Management, Chongqing Medical University, Chongqing, 400016, China. lige@vip.sina.com.
authors.forename: Ge
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Epidemiology, the Innovation Center for Social Risk Governance in Health, School of Public Health and Management, Chongqing Medical University, Chongqing, 400016, China. zhouli_tj@163.com.|Department of Epidemiology, School of Public Health and Management, Chongqing Medical University, Chongqing, 400016, China. zhouli_tj@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
mesh_terms: D000368:Aged; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D002681:China; D003327:Coronary Disease / Q000235:genetics*; D005260:Female; D056726:Genetic Association Studies; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors* / Q000235:genetics; D012737:Sex Factors; D014280:Triglycerides / Q000097:blood
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; C493170:TRIB1 protein, human; D014280:Triglycerides; D017346:Protein Serine-Threonine Kinases