title: Liquid chromatography-tandem mass spectrometry for the quantification of flurbiprofen in human plasma and its application in a study of bioequivalence.
abstract: A simple, quick and accurate LC-MS/MS method for the quantification of flurbiprofen in human plasma with indomethacin as internal standard (IS) was developed and validated. Samples were treated with methanol to precipitate proteins, then separated on a Ultimate C18 column (5μm, 2.1×50mm) with a gradient elusion process. Mobile phase A was comprised of water and formic acid, mobile phase B was comprised of acetonitrile and formic acid. Multi reaction monitoring (MRM) signals were saved on a negative ionization electrospray mass spectrometer. The calibration curve showed good linearity in the range of 40.00-10000.00μg/L (r(2)=0.998). Intra-day RE was 0.2-2.2%. Inter-day RE was 0.5-3.4%. The samples showed good stability under the study conditions. No significant matrix effect was observed. The established method was then applied to a bioequivalence study of a flurbiprofen axetil formulation.
authors.affiliation: Department of Pharmacology, Institute of Clinical Pharmacology, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, PR China. Electronic address: chenhuitjmu@163.com.
authors.forename: Chenghan
authors.initials: C
authors.lastname: Mei
authors.affiliation: Department of Pharmacology, Institute of Clinical Pharmacology, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: Institute of Clinical Pharmacy, Union Hospital Affiliated to Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430022, PR China.
authors.forename: Qiangfeng
authors.initials: Q
authors.lastname: Yin
authors.affiliation: Department of Pharmacology, Institute of Clinical Pharmacology, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Jin
authors.affiliation: Department of Pharmacology, Institute of Clinical Pharmacology, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, PR China.
authors.forename: Ting
authors.initials: T
authors.lastname: Xiong
authors.affiliation: Department of Pharmacology, Institute of Clinical Pharmacology, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, PR China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: He
authors.affiliation: Department of Pharmacology, Institute of Clinical Pharmacology, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, PR China.
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Gao
authors.affiliation: Department of Pharmacology, Institute of Clinical Pharmacology, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, PR China.
authors.forename: Rong
authors.initials: R
authors.lastname: Xu
authors.affiliation: Institute of Clinical Pharmacy, Union Hospital Affiliated to Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430022, PR China.
authors.forename: Piqi
authors.initials: P
authors.lastname: Zhou
authors.affiliation: Department of Pharmacology, Institute of Clinical Pharmacology, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, PR China.
authors.forename: Heng
authors.initials: H
authors.lastname: Zheng
authors.affiliation: Department of Pharmacology, Institute of Clinical Pharmacology, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, PR China; Institute of Clinical Pharmacy, Union Hospital Affiliated to Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430022, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
mesh_terms: D000328:Adult; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005480:Flurbiprofen / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D008297:Male; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013810:Therapeutic Equivalency
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D005480:Flurbiprofen
keywords: Bioequivalence; Flurbiprofen; Human plasma; LC-MS/MS; Pharmacokinetics