title: Complete genome sequence and intracellular protein localization of Datura yellow vein nucleorhabdovirus.
abstract: A limited number of plant rhabdovirus genomes have been fully sequenced, making taxonomic classification, evolutionary analysis and molecular characterization of this virus group difficult. We have for the first time determined the complete genome sequence of 13,188 nucleotides of Datura yellow vein nucleorhabdovirus (DYVV). DYVV genome organization resembles that of its closest relative, Sonchus yellow net virus (SYNV), with six ORFs in antigenomic orientation, separated by highly conserved intergenic regions and flanked by complementary 3' leader and 5' trailer sequences. As is typical for nucleorhabdoviruses, all viral proteins, except the glycoprotein, which is targeted to the endoplasmic reticulum, are localized to the nucleus. Nucleocapsid (N) protein, matrix (M) protein and polymerase, as components of nuclear viroplasms during replication, have predicted strong canonical nuclear localization signals, and N and M proteins exclusively localize to the nucleus when transiently expressed as GFP fusions. As in all nucleorhabdoviruses studied so far, N and phosphoprotein P interact when co-expressed, significantly increasing P nuclear localization in the presence of N protein. This research adds to the list of complete genomes of plant-infecting rhabdoviruses, provides molecular tools for further characterization and supports classification of DYVV as a nucleorhabdovirus closely related to but with some distinct differences from SYNV.
authors.affiliation: Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, QLD 4072, Australia. Electronic address: r.dietzgen@uq.edu.au.
authors.forename: Ralf G
authors.initials: RG
authors.lastname: Dietzgen
authors.affiliation: Queensland Department of Agriculture and Fisheries, Agri-Science Queensland, Crop and Food Science, Health and Food Sciences Precinct, Coopers Plains, QLD 4108, Australia. Electronic address: david.innes@daf.qld.gov.au.
authors.forename: David J
authors.initials: DJ
authors.lastname: Innes
authors.affiliation: Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, QLD 4072, Australia. Electronic address: n.bejerman@uq.edu.au.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Bejerman
mesh_terms: D001483:Base Sequence; D002467:Cell Nucleus / Q000821:virology*; D032302:Datura / Q000821:virology; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology; D021381:Protein Transport; D012209:Rhabdoviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D014764:Viral Proteins
keywords: Genome structure; Nucleorhabdovirus; Plant virus; Protein localization