title: Study of perfusion changes in the optic disc of patients with fibromyalgia syndrome using new colorimetric analysis software.
abstract: OBJECTIVE
  We measured the amount of hemoglobin at the optic nerve head of fibromyalgia (FM) patients using new colorimetric analysis software. We also investigated whether perfusion defects of the optic nerve head in patients with FM lead to tissue atrophy and corresponding retinal nerve fiber layer (RNFL) thinning measured by optical coherence tomography (OCT).
  
  
  METHODS
  We recruited for this cross-sectional study 118 FM patients and 76 sex- and age-matched healthy controls. All subjects underwent a complete neuro-ophthalmologic examination, which also included visual field testing using the Spark strategy in an Easyfield perimeter, and OCT examinations using the Spectralis. One photograph of the optic disc was obtained using a Cirrus™ Photo 800 multi-modality imager. We analyzed fundus photographs using Laguna ONhE software, a new method that allows hemoglobin levels to be measured at the optic nerve head. We compared hemoglobin percentages in different sectors of the nerve head and RNFL thicknesses between the two groups.
  
  
  RESULTS
  Mean hemoglobin percentages and hemoglobin content in all optic nerve head sectors calculated by the Laguna ONhE program were significantly lower in FM patients than in healthy controls, and the main differences were detected in the outer ring, which corresponds with the neuroretinal rim. However, only the differences in the superotemporal RNFL were statistically significant. Correlations between the RNFL thickness and the percentage of hemoglobin in the different sectors were weak.
  
  
  CONCLUSIONS
  Optic disc perfusion was decreased in patients with FM, especially within the neuroretinal rim, without clear involvement in the RNFL.
authors.affiliation: Ophthalmology Department, Miguel Servet University Hospital, Isabel la Catolica 1-3, 50009 Zaragoza, Spain; Aragones Institute of Health Sciences, Service, Lozano Blesa University Hospital Clinic, Avenida San Juan Bosco, C/Domingo Miral, s/n. 15, 50009 Zaragoza, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Pilar Bambo
authors.affiliation: Ophthalmology Department, Miguel Servet University Hospital, Isabel la Catolica 1-3, 50009 Zaragoza, Spain; Aragones Institute of Health Sciences, Service, Lozano Blesa University Hospital Clinic, Avenida San Juan Bosco, C/Domingo Miral, s/n. 15, 50009 Zaragoza, Spain. Electronic address: egmvivax@yahoo.com.
authors.forename: E
authors.initials: E
authors.lastname: Garcia-Martin
authors.affiliation: Ophthalmology Department, Miguel Servet University Hospital, Isabel la Catolica 1-3, 50009 Zaragoza, Spain; Aragones Institute of Health Sciences, Service, Lozano Blesa University Hospital Clinic, Avenida San Juan Bosco, C/Domingo Miral, s/n. 15, 50009 Zaragoza, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Gutierrez-Ruiz
authors.affiliation: Aragones Institute of Health Sciences, Service, Lozano Blesa University Hospital Clinic, Avenida San Juan Bosco, C/Domingo Miral, s/n. 15, 50009 Zaragoza, Spain; Centro Salud Arrabal, Red de Investigación en Atención primaria (REDIAPP), Zaragoza, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Magallon
authors.affiliation: Institut Universitari d'Investigació en Ciències de la Salut (IUNICS), University of Balearic Islands, Palma de Mallorca, Mallorca, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Roca
authors.affiliation: Aragones Institute of Health Sciences, Service, Lozano Blesa University Hospital Clinic, Avenida San Juan Bosco, C/Domingo Miral, s/n. 15, 50009 Zaragoza, Spain; Department of Psychiatry, Miguel Servet Hospital, University of Zaragoza, Red de Investigación en Atención primaria (REDIAPP), Zaragoza, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Garcia-Campayo
authors.affiliation: Ophthalmology Department, Miguel Servet University Hospital, Isabel la Catolica 1-3, 50009 Zaragoza, Spain; Aragones Institute of Health Sciences, Service, Lozano Blesa University Hospital Clinic, Avenida San Juan Bosco, C/Domingo Miral, s/n. 15, 50009 Zaragoza, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Perez-Olivan
authors.affiliation: Ophthalmology Department, Miguel Servet University Hospital, Isabel la Catolica 1-3, 50009 Zaragoza, Spain; Aragones Institute of Health Sciences, Service, Lozano Blesa University Hospital Clinic, Avenida San Juan Bosco, C/Domingo Miral, s/n. 15, 50009 Zaragoza, Spain.
authors.forename: V
authors.initials: V
authors.lastname: Polo
authors.affiliation: Ophthalmology Department, Miguel Servet University Hospital, Isabel la Catolica 1-3, 50009 Zaragoza, Spain; Aragones Institute of Health Sciences, Service, Lozano Blesa University Hospital Clinic, Avenida San Juan Bosco, C/Domingo Miral, s/n. 15, 50009 Zaragoza, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Larrosa
authors.affiliation: Ophthalmology Department, Miguel Servet University Hospital, Isabel la Catolica 1-3, 50009 Zaragoza, Spain; Aragones Institute of Health Sciences, Service, Lozano Blesa University Hospital Clinic, Avenida San Juan Bosco, C/Domingo Miral, s/n. 15, 50009 Zaragoza, Spain.
authors.forename: L E
authors.initials: LE
authors.lastname: Pablo
mesh_terms: D000328:Adult; D001775:Blood Circulation; D016022:Case-Control Studies; D003124:Colorimetry / Q000379:methods*; D005260:Female; D005356:Fibromyalgia / Q000150:complications / Q000503:physiopathology*; D006452:Hemoglobinometry / Q000379:methods*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007429:Intraocular Pressure; D008297:Male; D008875:Middle Aged; D009896:Optic Atrophy / Q000175:diagnosis* / Q000209:etiology / Q000503:physiopathology; D009898:Optic Disk / Q000098:blood supply* / Q000473:pathology; D018917:Optic Neuropathy, Ischemic / Q000175:diagnosis* / Q000209:etiology / Q000503:physiopathology; D010781:Photography; D012907:Smoking / Q000503:physiopathology; D012984:Software; D041623:Tomography, Optical Coherence
keywords: Disque optique; Fibres nerveuses rétiniennes; Fibromyalgia; Fibromyalgie; Hemoglobin levels; Optic disc; Optic nerve head; Optical coherence tomography; Retinal nerve fiber layer; Taux d’hémoglobine; Tomographie par cohérence optique; Tête du nerf optique