title: Genetic encoding of the post-translational modification 2-hydroxyisobutyryl-lysine.
abstract: We report the synthesis and genetic encoding of a recently discovered post-translational modification, 2-hydroxyisobutyryl-lysine, to the genetic code of E. coli. The production of homogeneous proteins containing this amino acid will facilitate the study of modification in full-length proteins.
authors.affiliation: Department of Chemistry, Virginia Commonwealth University, 1001 W. Main Street, Richmond, Virginia 23284, USA. tacropp@vcu.edu.
authors.forename: William A
authors.initials: WA
authors.lastname: Knight
authors.forename: T Ashton
authors.initials: TA
authors.lastname: Cropp
mesh_terms: D000595:Amino Acid Sequence; D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008239:Lysine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008250:Lysine-tRNA Ligase / Q000235:genetics / Q000378:metabolism*; D017020:Methanosarcina / Q000201:enzymology; D008969:Molecular Sequence Data; D011499:Protein Processing, Post-Translational; D013379:Substrate Specificity
chemical_list: C000605889:2-hydroxyisobutyryl-lysine; D029968:Escherichia coli Proteins; D049452:Green Fluorescent Proteins; D000604:Amino Acyl-tRNA Synthetases; D008250:Lysine-tRNA Ligase; D008239:Lysine