title: Effect of a combination of calorie-restriction therapy and Lingguizhugan decoction on levels of fasting blood lipid and inflammatory cytokines in a high-fat diet induced hyperlipidemia rat model.
abstract: OBJECTIVE
  To combine calorie-restriction therapy (CRT) with Traditional Chinese Medicine (TCM) using Lingguizhugan Decoction and note the effects on expression of inflammatory cytokines [tumor necrosis factor (TNF)-α, high-sensitivity C-reactive protein (hs-CRP), interleukin (IL)-6] in high-fat-diet induced hyperlipidemia in rats.
  
  
  METHODS
  Forty rats were divided randomly and equally into four groups: control group A (regular feeding for 5 weeks); model group B (high-fat diet for 5 weeks), calorie-limited group C (high-fat diet for 5 weeks) and TCM calorie-limit group D (high-fat diet for 5 weeks). After modeling, groups C and D were fasted for 3 days: group C with simple fasting, and group D with TCM fasting. The motion as well as changes in color, body weight, food intake, plasma lipids [low-density lipoprotein-cholesterol (LDL-C), high-density lipoprotein-cholesterol, total cholesterol (TC) and triglyceride (TG) along with TNF-α, hs-CRP and IL-6] were measured before and after intervention.
  
  
  RESULTS
  Modeled rats were established after five weeks. After 3 days of fasting, compared with group B, blood lipid levels (TG, TC, LDL-C) of groups C and D decreased dramatically. Those of group D decreased more obviously than those of group C. However, the values of TNF-α, hs-CRP and IL-6 showed no obvious difference between each of the three treatment groups.
  
  
  CONCLUSIONS
  CRT can reduce fasting blood lipid levels in rats, but not by reducing the levels of inflammatory cytokines.
authors.forename: Wang
authors.initials: W
authors.lastname: Yuanyuan
authors.forename: Jin
authors.initials: J
authors.lastname: Minghua
authors.forename: Zhang
authors.initials: Z
authors.lastname: Lina
authors.forename: Li
authors.initials: L
authors.lastname: Suhua
authors.forename: Zhai
authors.initials: Z
authors.lastname: Jiayu
authors.forename: Shen
authors.initials: S
authors.lastname: Yongzhi
authors.forename: Chen
authors.initials: C
authors.lastname: Chunyu
authors.forename: Qin
authors.initials: Q
authors.lastname: Jian
mesh_terms: D000818:Animals; D002097:C-Reactive Protein / Q000032:analysis; D031204:Caloric Restriction; D002784:Cholesterol / Q000097:blood; D003131:Combined Modality Therapy; D016207:Cytokines / Q000097:blood*; D059305:Diet, High-Fat / Q000009:adverse effects; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood / Q000178:diet therapy* / Q000188:drug therapy*; D018836:Inflammation Mediators / Q000097:blood*; D015850:Interleukin-6 / Q000097:blood; D008055:Lipids / Q000097:blood*; D008297:Male; D051381:Rats; D014280:Triglycerides / Q000097:blood
chemical_list: D016207:Cytokines; D004365:Drugs, Chinese Herbal; D018836:Inflammation Mediators; D015850:Interleukin-6; D008055:Lipids; D014280:Triglycerides; D002097:C-Reactive Protein; D002784:Cholesterol