title: Effect of in vitro and in vivo conditions on development of parthenogenetic rabbit embryos after vitrification.
abstract: Parthenote embryos offer multiple opportunities in biotechnological research, so it is important to analyse the possibilities for their cryopreservation in order to establish a biobank. The aim of this experiment was to determine the effect of culture conditions and vitrification on rabbit parthenogenetic embryos. Parthenotes were cultured under in vivo and in vitro conditions until day 3 (late morula/early blastocyst), when they were vitrified. Immediately after warming, they were newly cultured under in vivo and in vitro conditions till day 6 (blastocyst stage). Both culture conditions showed similar late morula/early blastocyst (0.39±0.056 vs. 0.46±0.043, for in vivo and in vitro, respectively) and blastocyst rates (0.12±0.068 vs. 0.13±0.070, for in vivo and in vitro, respectively). However, no parthenote was recovered when a combination of culture conditions was performed. To our best knowledge, this is the first demonstration of the ability of rabbit parthenogenetic embryos to develop after vitrification, with similar embryo development after in vivo or in vitro culture. Nevertheless, our results highlight the importance of culture conditions on the morphology of parthenote embryos. Therefore, we have described that special attention should be paid on culture conditions to generate parthenote embryos, with a view to their subsequent use, for example in embryonic stem cell production.
authors.affiliation: Instituto de Ciencia y Tecnología Animal, Laboratorio de Biotecnología de la Reproducción, Universidad Politécnica de Valencia, 46022 Valencia, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Naturil-Alfonso
authors.affiliation: Instituto de Ciencia y Tecnología Animal, Laboratorio de Biotecnología de la Reproducción, Universidad Politécnica de Valencia, 46022 Valencia, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Jiménez-Trigos
authors.affiliation: Instituto de Ciencia y Tecnología Animal, Laboratorio de Biotecnología de la Reproducción, Universidad Politécnica de Valencia, 46022 Valencia, Spain.
authors.forename: J S
authors.initials: JS
authors.lastname: Vicente
authors.affiliation: Instituto de Ciencia y Tecnología Animal, Laboratorio de Biotecnología de la Reproducción, Universidad Politécnica de Valencia, 46022 Valencia, Spain. Electronic address: fmarco@dca.upv.es.
authors.forename: F
authors.initials: F
authors.lastname: Marco-Jiménez
mesh_terms: D000818:Animals; D018070:Biological Specimen Banks; D001755:Blastocyst / Q000166:cytology; D015925:Cryopreservation / Q000379:methods*; D046149:Embryo Culture Techniques / Q000379:methods*; D004622:Embryo, Mammalian / Q000196:embryology; D047108:Embryonic Development / Q000502:physiology*; D009028:Morula / Q000166:cytology; D010312:Parthenogenesis / Q000502:physiology*; D011817:Rabbits; D058989:Vitrification
keywords: In vitro; In vivo; Parthenogenesis; Rabbit; Vitrification