title: Antimicrobial, antitumor and brine shrimp lethality assay of Ranunculus arvensis L. extracts.
abstract: To investigate the antitumor activity, brine shrimp lethality assay, antibacterial and antifungal activity of Methanol Extract (ME), Water Extract (WE), Acetone Extract (AE), Chloroform Extract (CE), Methanol-Water Extract (MWE), Methanol-Acetone Extract (MAE), Methanol-Chloroform Extract (MCE) of Ranunculus arvensis (L.). Antitumor activity was evaluated with Agrobacterium tumefaciens (At10) induced potato disc assay. Cytotoxicity was evaluated with brine shrimp lethality assay. Antibacterial activity was evaluated with six bacterial strains including Escherichia coli, Enterobacter aerogenes, Bordetella bronchiseptica, Klebsiella pneumoniae, Micrococcus luteus and Streptococcus anginosus and antifungal screening was done against five fungal strains including Aspergillus niger, A. flavus, A. fumigates, Fusarium solani and Mucor species by using disc diffusion method. Best antitumor activity was obtained with ME and WE, having highest IC50 values 20.27 ± 1.62 and 93.01 ± 1.33μg/disc. Brine shrimp lethality assay showed LC50 values of AE, MAE and ME were obtained as 384.66 ± 9.42μg/ml, 724.11 ± 8.01μg/ml and 978.7 ±8.01 μg/ml respectively. WE of R. arvensis revealed weak antimicrobial result against the tested microorganisms. On the other hand, the antifungal activity of the plant extracts was found to be insignificant. These findings demonstrate that extracts of R. arvensis possesses significant antitumor activity. Further extensive study is necessary to assess the therapeutic potential of the plant.
authors.affiliation: Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan / Institute of Biomedical Sciences, School of Life Science, East China Normal University, Dongchuan Road, Shanghai, China.
authors.forename: Muhammad Zeeshan
authors.initials: MZ
authors.lastname: Bhatti
authors.affiliation: Institute of Biomedical Sciences, School of Life Science, East China Normal University, Dongchuan Road, Shanghai, China.
authors.forename: Amjad
authors.initials: A
authors.lastname: Ali
authors.affiliation: Department of Biological Sciences, Gomal University, Dera Ismail Khan, Pakistan.
authors.forename: Asma
authors.initials: A
authors.lastname: Saeed
authors.affiliation: Department of Chemistry, University of Science & Technology, Bannu, Pakistan.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Saeed
authors.affiliation: Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
authors.forename: Salman Akbar
authors.initials: SA
authors.lastname: Malik
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D001156:Artemia / Q000187:drug effects*; D001230:Aspergillus / Q000187:drug effects; D016950:Bordetella bronchiseptica / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D021902:Enterobacter aerogenes / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D005670:Fusarium / Q000187:drug effects; D007711:Klebsiella pneumoniae / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D016982:Micrococcus luteus / Q000187:drug effects; D009089:Mucor / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D031947:Ranunculus; D034366:Streptococcus anginosus / Q000187:drug effects
chemical_list: D000890:Anti-Infective Agents; D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts