title: [Isolation and identification of mosquito-borne arboviruses in Yuncheng city, Shanxi province, 2012].
abstract: OBJECTIVE
  To investigate the species and distribution of mosquitoes and mosquito-borne arboviruses in Yuncheng city of Shanxi province, China.
  
  
  METHODS
  Mosquito samples were collected in 19 collection sites from Linyi county and Yongji city in Yuncheng city, in August, 2012. After identification and classification, all the specimens were homogenized and centrifuged to acquire supernatant before being inoculated to both C6/36 and BHK21 cells for viral isolation. Positive isolates were identified with arbovirus species-specific primers under RT-PCR, for further sequencing and phylogenetic analysis.
  
  
  RESULTS
  A total of 10 455 mosquitoes of 7 species in 4 genuese were collected. The predominant mosquito species in Linyi county was Culex pipens pallens (91.96%, 3 911/4 253), but the one in Yongji city was Culex tritaeniorhynchus (72.85%, 4 518/6 202). A total of 23 strains of viruses were isolated from the mosquito pools. 15 strains from Culex tritaeniorhynchus and Culex pipens pallens were identified as genotype I Japanese encephalitis virus (JEV). Four strains from Culex pipens pallens were identified as Culex flavivirus (CxFV). Three strains from Culex pipens pallens were identified as Culex pipiens pallens densovirus (CppDNV). One strain from Armigeres subalbatus and Aedes albopictus was identified as Getah virus (GETV).
  
  
  CONCLUSIONS
  Four kinds of arboviruses were isolated from the mosquito pools, including GETV and CxFV, which were isolated and documented in Shanxi province for the first time. In the city of Yuncheng, Culex tritaeniorhynchus had been the predominant species and major vector for transmitting JEV. Genotype I JEV remained the major JEV circulating in the local natural environment.
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
authors.forename: Yayun
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
authors.forename: Yuxi
authors.initials: Y
authors.lastname: Cao
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
authors.forename: Shihong
authors.initials: S
authors.lastname: Fu
authors.affiliation: Shanxi Provincial Center for Disease Control and Prevention.
authors.forename: Jingxia
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Shanxi Provincial Center for Disease Control and Prevention.
authors.forename: Junying
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Shanxi Provincial Center for Disease Control and Prevention.
authors.forename: Peifang
authors.initials: P
authors.lastname: Dai
authors.affiliation: Shanxi Provincial Center for Disease Control and Prevention.
authors.forename: Xiangsheng
authors.initials: X
authors.lastname: Kong
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China; Email: gdliang@hotmail.com.
authors.forename: Guodong
authors.initials: G
authors.lastname: Liang
mesh_terms: D000818:Animals; D001103:Arboviruses / Q000235:genetics* / Q000302:isolation & purification*; D002681:China; D002947:Cities; D009033:Culicidae / Q000821:virology*; D004664:Encephalitis Virus, Japanese / Q000235:genetics; D010802:Phylogeny; D013045:Species Specificity