title: Toll-like Receptor 10 in Helicobacter pylori Infection.
abstract: Innate immunity plays important roles in the primary defense against pathogens, and epidemiological studies have suggested a role for Toll-like receptor 1 (TLR1) in Helicobacter pylori susceptibility. Microarray analysis of gastric biopsy specimens from H. pylori-positive and uninfected subjects showed that TLR10 messenger RNA (mRNA) levels were upregulated approximately 15-fold in infected subjects; these findings were confirmed by real-time quantitative polymerase chain reaction analysis. Immunohistochemical investigation showed increased TLR10 expression in the gastric epithelial cells of infected individuals. When H. pylori was cocultured with NCI-N87 gastric cells, both TLR10 and TLR2 mRNA levels were upregulated. We compared the ability of TLR combinations to mediate nuclear factor-κB (NF-κB) activation. Compared with other TLR2 subfamily heterodimers, the TLR2/TLR10 heterodimer mediated the greatest NF-κB activation following exposure to heat-killed H. pylori or H. pylori lipopolysaccharide. We conclude that TLR10 is a functional receptor involved in the innate immune response to H. pylori infection and that the TLR2/TLR10 heterodimer functions in H. pylori lipopolysaccharide recognition.
authors.affiliation: Department of Environmental and Preventive Medicine Department of Medicine-Gastroenterology, Michael E. DeBakey Veterans Affairs Medical Center and Baylor College of Medicine, Houston, Texas.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nagashima
authors.affiliation: Department of Environmental and Preventive Medicine Department of Medicine-Gastroenterology, Michael E. DeBakey Veterans Affairs Medical Center and Baylor College of Medicine, Houston, Texas.
authors.forename: Shun
authors.initials: S
authors.lastname: Iwatani
authors.affiliation: Institute of Microbiology and Parasitology, Department of Science, Autonomous University of Santo Domingo Department of Biomedical Research, School of Medicine, Santiago Technological University.
authors.forename: Modesto
authors.initials: M
authors.lastname: Cruz
authors.affiliation: Dominican-Japanese Digestive Disease Center, Dr Luis E. Aybar Health and Hygiene City, Santo Domingo, Dominican Republic.
authors.forename: José A
authors.initials: JA
authors.lastname: Jiménez Abreu
authors.affiliation: Department of Molecular Pathology, Oita University Faculty of Medicine, Yufu, Japan.
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Uchida
authors.affiliation: Gastroenterology Unit, Department of Medicine, Thammasat University Hospital, Pathumthani.
authors.forename: Varocha
authors.initials: V
authors.lastname: Mahachai
authors.affiliation: Department of Gastroenterology, Bangkok Hospital, Thailand.
authors.forename: Ratha-Korn
authors.initials: RK
authors.lastname: Vilaichone
authors.affiliation: Department of Medicine-Gastroenterology, Michael E. DeBakey Veterans Affairs Medical Center and Baylor College of Medicine, Houston, Texas.
authors.forename: David Y
authors.initials: DY
authors.lastname: Graham
authors.affiliation: Department of Environmental and Preventive Medicine Department of Medicine-Gastroenterology, Michael E. DeBakey Veterans Affairs Medical Center and Baylor College of Medicine, Houston, Texas.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Yamaoka
mesh_terms: D002460:Cell Line; D018920:Coculture Techniques; D004847:Epithelial Cells / Q000737:chemistry; D005753:Gastric Mucosa / Q000473:pathology; D020869:Gene Expression Profiling; D016481:Helicobacter Infections / Q000276:immunology*; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D008070:Lipopolysaccharides / Q000276:immunology; D046228:Microarray Analysis; D016328:NF-kappa B / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D051201:Toll-Like Receptor 10 / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D008070:Lipopolysaccharides; D016328:NF-kappa B; C475955:TLR10 protein, human; D051201:Toll-Like Receptor 10
keywords: H. pylori LPS; Helicobacter pylori; TLR10; TLR2/TLR10 heterodimer; innate immunity