title: Green synthesis of peptide-templated gold nanoclusters as novel fluorescence probes for detecting protein kinase activity.
abstract: A green method was employed for synthesizing peptide-templated nanoclusters without requiring strong reducing agents. Using synthetic peptide-gold nanoclusters as fluorescence probes, a novel assay for detecting protein kinase is developed based on phosphorylation against carboxypeptidase Y digestion.
authors.affiliation: Department of Chemistry, Nanchang University, Nanchang 330031, China. jdqiu@ncu.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Song
authors.forename: Ru-Ping
authors.initials: RP
authors.lastname: Liang
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Jian-Ding
authors.initials: JD
authors.lastname: Qiu
mesh_terms: D000595:Amino Acid Sequence; D043402:Cathepsin A / Q000378:metabolism; D060326:Chemistry Techniques, Synthetic; D057075:Enzyme Assays / Q000379:methods*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D055772:Green Chemistry Technology; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D008958:Models, Molecular; D008968:Molecular Conformation; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000737:chemistry* / Q000378:metabolism; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D009842:Oligopeptides; D006046:Gold; D011494:Protein Kinases; D043402:Cathepsin A