title: Development of a quantitative method for the simultaneous analysis of the boar taint compounds androstenone, skatole and indole in porcine serum and plasma by means of ultra-high performance liquid chromatography coupled to high resolution mass spectrometry.
abstract: Boar taint is an off-odour occurring while heating meat or fat from boars. A method detecting the three compounds (androstenone, skatole and indole) simultaneously in blood would offer substantial advantages since it would allow monitoring the impact of rearing strategies. Therefore, a UHPLC-HR-Orbitrap-MS analysis method is optimized and validated for the quantification of these compounds in plasma or serum. Sample pre-treatment involved an extraction with diethylether followed by a centrifugal filtration (30 kDa). Limits of detection and quantification varied between 0.5 and 1 μg L(-1) and 2 and 3 μg L(-1) for the three compounds, respectively. Besides, an excellent repeatability (RSD < 7.6%), within-laboratory reproducibility (RSD<10.5%), recovery (87-97%) and linearity (R(2)>0.99) were recorded. Correlations between serum/plasma and fat levels of the boar taint compounds were positive for skatole (r(serum) = 0.39 and r(plasma) = 0.84) and androstenone (r(serum) = 0.73-0.78 and r(plasma) = 0.32-0.80).
authors.affiliation: Ghent University, Faculty of Veterinary Medicine, Department of Veterinary Public Health and Food Safety, Laboratory of Chemical Analysis, Salisburylaan 133, B-9820 Merelbeke, Belgium. Electronic address: Jella.Wauters@ugent.be.
authors.forename: J
authors.initials: J
authors.lastname: Wauters
authors.affiliation: Ghent University, Faculty of Veterinary Medicine, Department of Veterinary Public Health and Food Safety, Laboratory of Chemical Analysis, Salisburylaan 133, B-9820 Merelbeke, Belgium. Electronic address: Julie.VandenBussche@ugent.be.
authors.forename: J
authors.initials: J
authors.lastname: Vanden Bussche
authors.affiliation: Ghent University, Faculty of Veterinary Medicine, Department of Veterinary Public Health and Food Safety, Laboratory of Chemical Analysis, Salisburylaan 133, B-9820 Merelbeke, Belgium. Electronic address: Kaat.Verplanken@ugent.be.
authors.forename: K
authors.initials: K
authors.lastname: Verplanken
authors.affiliation: Ghent University, Faculty of Veterinary Medicine, Department of Veterinary Public Health and Food Safety, Laboratory of Chemical Analysis, Salisburylaan 133, B-9820 Merelbeke, Belgium. Electronic address: Karenbekaert@gmail.com.
authors.forename: K M
authors.initials: KM
authors.lastname: Bekaert
authors.affiliation: Institute of Agricultural and Fisheries Research (ILVO), Animal Sciences Unit, Scheldeweg 68, B-9090 Melle, Belgium. Electronic address: Marijke.Aluwe@ilvo.vlaanderen.be.
authors.forename: M
authors.initials: M
authors.lastname: Aluwé
authors.affiliation: Institute of Agricultural and Fisheries Research (ILVO), Animal Sciences Unit, Scheldeweg 68, B-9090 Melle, Belgium. Electronic address: Alice.Vandenbroeke@ilvo.vlaanderen.be.
authors.forename: A
authors.initials: A
authors.lastname: Van den Broeke
authors.affiliation: KU Leuven University, Science, Engineering and Technology Group, Department of Biosystems (BIOSYST), Division of Gene Technology, Kasteelpark Arenberg 21, B-3001 Heverlee, Belgium. Electronic address: Annelies.Cousse@biw.kuleuven.be.
authors.forename: A
authors.initials: A
authors.lastname: Coussé
authors.affiliation: KU Leuven University, Science, Engineering and Technology Group, Department of Biosystems (BIOSYST), Division of Gene Technology, Kasteelpark Arenberg 21, B-3001 Heverlee, Belgium. Electronic address: Nadine.Buys@biw.kuleuven.be.
authors.forename: N
authors.initials: N
authors.lastname: Buys
authors.affiliation: Ghent University, Faculty of Veterinary Medicine, Department of Veterinary Public Health and Food Safety, Laboratory of Chemical Analysis, Salisburylaan 133, B-9820 Merelbeke, Belgium. Electronic address: Lynn.Vanhaecke@ugent.be.
authors.forename: L
authors.initials: L
authors.lastname: Vanhaecke
mesh_terms: D000736:Androstenes / Q000097:blood*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007211:Indoles / Q000097:blood*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D008460:Meat / Q000032:analysis; D009812:Odorants / Q000032:analysis*; D012862:Skatole / Q000097:blood*; D013552:Swine / Q000097:blood*
chemical_list: D000736:Androstenes; D007211:Indoles; C000214:androst-16-en-3-one; C030374:indole; D012862:Skatole
keywords: Blood; Correlation; Fat; LC–MS; Off-odour; Orbitrap; Pig