title: Identification of target antigens of naturally occurring autoantibodies in cerebrospinal fluid.
abstract: Naturally occurring autoantibodies have natural physiologic functions related to normal cell processes. However, the repertoire of naturally occurring autoantibodies against neuronal antigens in CSF is unclear. The purpose of this study was to identify naturally occurring autoantibodies against neuronal antigens in CSF from patients with various neurologic diseases by proteomics-based analysis. The CSF samples were collected from 77 patients with various neurologic disorders. The antigen source for 2-dimensional immunoblotting was the SH-SY5Y human neuroblastoma cell line. There were 8 spots recognized in CSF from more than one-fourth of the 77 patients including all patient groups and these spots were recognized in intravenous immunoglobulin preparations. These antigen spots were identified as heat shock 105-kDa/110-kDa protein 1, isoform CRA_b, 78-kDa glucose-regulated protein, heat shock cognate 71-kDa protein, tubulin beta chain, vimentin (2 spots), and 60-kDa heat shock protein, mitochondrial; we could not identify the protein name corresponding to 1 of the 8 spots. In summary, there were 6 proteins identified that were main target antigens that reacted with naturally occurring autoantibodies in CSF from patients with varied neurologic disorders; the functions of autoantibodies against the identified antigens are unknown and may be clarified with further studies.
  
  
  CONCLUSIONS
  Naturally occurring autoantibodies may have important functions in tissue homeostasis. In this study, we identified 6 common target antigens that reacted with autoantibodies in cerebrospinal fluid (CSF) from patients, independent of disease type. These findings may clarify the importance of naturally occurring autoantibodies in CSF and the use of these antibodies potentially may be a novel therapy for various neurologic disorders.
authors.affiliation: Department of Neurology and Geriatrics, Gifu University Graduate School of Medicine, Gifu City, Gifu, Japan. Electronic address: kimura1@gifu-u.ac.jp.
authors.forename: Akio
authors.initials: A
authors.lastname: Kimura
authors.affiliation: Department of Neurology and Geriatrics, Gifu University Graduate School of Medicine, Gifu City, Gifu, Japan.
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Yoshikura
authors.affiliation: Department of Neurology and Geriatrics, Gifu University Graduate School of Medicine, Gifu City, Gifu, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Koumura
authors.affiliation: Department of Neurology and Geriatrics, Gifu University Graduate School of Medicine, Gifu City, Gifu, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Hayashi
authors.affiliation: Life Science Research Center, Center for Molecular Biology and Genetics, Mie University, Tsu City, Mie, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Kobayashi
authors.affiliation: Life Science Research Center, Center for Molecular Biology and Genetics, Mie University, Tsu City, Mie, Japan.
authors.forename: Issei
authors.initials: I
authors.lastname: Kobayashi
authors.affiliation: Graduate School of Regional Innovation Studies, Mie University, Tsu City, Mie, Japan.
authors.forename: Takeo
authors.initials: T
authors.lastname: Yano
authors.affiliation: Department of Neurology and Geriatrics, Gifu University Graduate School of Medicine, Gifu City, Gifu, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Inuzuka
mesh_terms: D000941:Antigens / Q000737:chemistry* / Q000276:immunology*; D001323:Autoantibodies / Q000737:chemistry* / Q000276:immunology*; D002555:Cerebrospinal Fluid / Q000737:chemistry / Q000276:immunology*; D005260:Female; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D008297:Male; D009422:Nervous System Diseases / Q000134:cerebrospinal fluid*
chemical_list: D000941:Antigens; D001323:Autoantibodies
keywords: Heat shock protein; Neuron; Proteomics; Tubulin; Vimentin