title: Different serotypes of dengue viruses differently regulate the expression of the host cell antigen processing machinery.
abstract: Dengue virus (DV) infection demonstrates an intriguing virus-induced intracellular membrane alteration that results in the augmentation of major histocompatibility complex (MHC) class I-restricted antigen presentation. As oppose to its biological function in attracting CD8(+) T-cells, this phenomenon appears to facilitate the immune evasion. However, the molecular events that attribute to the dysregulation of the antigen presenting mechanism (APM) by DV remain obscure. In this study, we aimed to characterize the host cell APM upon infection with all serotypes of whole DV. Cellular RNA were isolated from infected cells and the gene expressions of LMP2, LMP7, TAP1, TAP2, TAPBP, CALR, CANX, PDIA3, HLA-A and HLA-B were analyzed via quantitative PCR. The profiles of the gene expression were further validated. We showed that all four DV serotypes modulate host APM at the proteasomal level with DV2 showing the most prominent expression profile.
authors.affiliation: Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia; Drug Design and Development Research Group, University of Malaya, 50603 Kuala Lumpur, Malaysia. Electronic address: mr.dennisgan@gmail.com.
authors.forename: Chye Sheng
authors.initials: CS
authors.lastname: Gan
authors.affiliation: Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia; Drug Design and Development Research Group, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Rohana
authors.initials: R
authors.lastname: Yusof
authors.affiliation: Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia; Drug Design and Development Research Group, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Shatrah
authors.initials: S
authors.lastname: Othman
mesh_terms: D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D000071450:ATP Binding Cassette Transporter, Subfamily B, Member 3; D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D017951:Antigen Presentation / Q000235:genetics* / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes; D003716:Dengue Virus / Q000276:immunology*; D005786:Gene Expression Regulation; D015395:Histocompatibility Antigens Class I / Q000235:genetics* / Q000276:immunology; D006801:Humans; D057131:Immune Evasion / Q000235:genetics / Q000276:immunology; D026901:Membrane Transport Proteins / Q000235:genetics; D046988:Proteasome Endopeptidase Complex / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*; D065288:Serogroup
chemical_list: D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D000071450:ATP Binding Cassette Transporter, Subfamily B, Member 3; D018528:ATP-Binding Cassette Transporters; D015395:Histocompatibility Antigens Class I; D026901:Membrane Transport Proteins; D012333:RNA, Messenger; C073119:TAP1 protein, human; C107762:tapasin; C071692:TAP2 protein, human; D046988:Proteasome Endopeptidase Complex
keywords: Antigen presentation mechanism; Class I; Dengue; Major histocompatibility complex; Virus