title: Urinary metabolomic strategy to evaluate Compound Danshen Dripping Pills for myocardial ischaemia in rats.
abstract: Compound Danshen Dripping Pill (CDDP) has been used for the treatment of coronary heart disease for decades. We aimed to increase the understanding of the mechanisms by evaluating the urinary metabolomics of CDDP using Gas Chromatography-Mass Spectrometer (GC-MS) in a myocardial ischaemia (MI) rat model. One hundred Sprague-Dawley rats were divided into Con (normal saline and no surgery), Con+ (107 mg/kg d CDDP solution and no surgery), Sham (normal saline and surgery without aorta ligation), Mod (normal saline and surgery with aorta ligation), and Mod+ (107 mg/kg d CDDP solution and surgery with aorta ligation) groups. Urine samples on days 0, 3, 14, and 28 were tested using GC-MS and analyzed with PCA and partial least squares-discriminant analysis models. In the Mod group, creatine kinase and malondialdehyde levels were higher, and superoxide-dismutase levels were lower; the same variables normalized in the Mod+ group. CDDP resulted in improvement in the Mod+ group, as indicated by the reduced necrosis in the myocardial tissue. A total of 36 metabolites were identified in the urine samples, and 8 metabolites (malate, succinate, creatinine, methionine, cysteine, serine, phenylalanine, and tyrosine) were increased remarkably and recovered to normal levels after treatment with CDDP. Differentially expressed metabolites implied that energy, amino acid, fatty acid, and polyol metabolism might be disrupted by MI and reversed by CDDP. Urinary metabolomics provide a dynamic monitoring approach that highlights interference by MI and the therapeutic effects of CDDP on MI in rats throughout the recovery process.
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China; School of Pharmacy, Shandong Wanjie Medical Institute, Zibo 255213, China.
authors.forename: Hai-Miao
authors.initials: HM
authors.lastname: Zou
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Biao
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Traditional Chinese Medicine, Shanghai Municipal Xuhui District Central Hospital, Shanghai 201620, China.
authors.forename: Xin-Chun
authors.initials: XC
authors.lastname: Xu
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Su
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Sun
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Song
authors.initials: S
authors.lastname: Xue
authors.affiliation: Ministry of Education Key Laboratory of Systems Biomedicine, Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: cathywxy@sjtu.edu.cn.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: mfqiu@sjtu.edu.cn.
authors.forename: Ming-Feng
authors.initials: MF
authors.lastname: Qiu
mesh_terms: D000818:Animals; D003402:Creatine Kinase / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D055432:Metabolomics / Q000379:methods; D017202:Myocardial Ischemia / Q000378:metabolism* / Q000652:urine*; D009206:Myocardium / Q000378:metabolism*; D009336:Necrosis / Q000378:metabolism / Q000652:urine; D051381:Rats; D017207:Rats, Sprague-Dawley; D027561:Salvia miltiorrhiza; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; C028972:dan-shen root extract; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D003402:Creatine Kinase
keywords: Compound Danshen Dripping Pills; Gas Chromatography-Mass Spectrometer; Myocardial ischaemia; Traditional Chinese medicine; Urinary metabolomics