title: Bioactivities of phytochemicals in Araiostegia yunnanensis (Christ).
abstract: The profile and bioactivity of phytochemicals in Araiostegia yunnanensis (Christ) Cop were investigated. The total flavonoids content in A. yunnanensis is about 84.90 mg/g. By means of HPLC-DAD-ESI-MS, the main flavonoids in A. yunnanensis were tentatively identified as myricetin 3-O-rhamnosylglucoside, eriodictyol 7-O-rutinoside, quercetin 3-O-rutinoside, luteolin-7-O-apiosylglucoside, quercetin 3-O-rhamnosylgalactoside, and luteolin 7-O-glucoside. The extract (0.268 mg/ml total flavonoids) from A. yunnanensis showed very strong superoxide anion radical scavenging potential and reducing power, which are higher than those of rutin (0.25 mg/ml). The extract (0.268 mg/ml total flavonoids) from A. yunnanensis exhibited similar DPPH scavenging activity with rutin (0.25 mg/ml). However, rutin (0.25 mg/ml) showed a significantly higher ABTS radical scavenging effect than that of the extract (0.268 mg/ml total flavonoids) from A. yunnanensis. The methanol extract from A. yunnanensis showed obviously cytotoxic effects on A549 cells and it had no effect against acetylcholinesterase.
authors.affiliation: College of Tea and Food Science & Technology, Anhui Agricultural University, 230036 Hefei, Anhui, China; Research Center of Engineering Technology for Extraction of Bioactive Compounds, Anhui Academy of Applied Technology, Suixi Road 312, 230031 Hefei, Anhui, China.
authors.forename: Longsheng
authors.initials: L
authors.lastname: Chen
authors.affiliation: Yancheng Institute of Technology, 224051 Yancheng, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.affiliation: Yancheng Institute of Technology, 224051 Yancheng, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Shao
authors.affiliation: College of Tea and Food Science & Technology, Anhui Agricultural University, 230036 Hefei, Anhui, China. Electronic address: aatanhui@yahoo.com.
authors.forename: Xianfeng
authors.initials: X
authors.lastname: Du
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry; D000975:Antioxidants / Q000737:chemistry; D045744:Cell Line, Tumor; D002800:Cholinesterase Inhibitors / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004354:Drug Screening Assays, Antitumor; D019669:Embryophyta / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry; D006801:Humans; D047311:Luteolin / Q000737:chemistry; D000432:Methanol / Q000737:chemistry; D064209:Phytochemicals / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D012431:Rutin / Q000737:chemistry; D013481:Superoxides / Q000737:chemistry
chemical_list: D000975:Antioxidants; D002800:Cholinesterase Inhibitors; D005419:Flavonoids; D064209:Phytochemicals; D010936:Plant Extracts; D013481:Superoxides; D012431:Rutin; C040015:myricetin; D000110:Acetylcholinesterase; D047311:Luteolin; D000432:Methanol
keywords: Anticancer; Antioxidant; Araiostegia yunnanensis; Flavonoids; HPLC–DAD–ESI-MS