title: Target of Rapamycin Complex 2 regulates cell growth via Myc in Drosophila.
abstract: Target of rapamycin (TOR) is an evolutionarily conserved serine/threonine protein kinase that functions as a central regulator of cellular growth and metabolism by forming two distinct complexes: TOR complex 1 (TORC1) and TORC2. As well as TORC1, TORC2 plays a key role in regulation of cell growth. But little is known about how TORC2 regulates cell growth. The transcription factor Myc also plays a critical role in cell proliferation and growth. Here we report that TORC2 and Myc regulate cell growth via a common pathway. Expression of Myc fully rescued growth defects associated with lst8 and rictor mutations, both of which encode essential components of TORC2. Furthermore, loss of TORC2 disrupted the nuclear localization of Myc, and inhibited Myc-dependent transcription. Together, our results reveal a Myc-dependent pathway by which TORC2 regulates cell growth.
authors.affiliation: National Institute of Biological Sciences, Beijing, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Kuo
authors.affiliation: National Institute of Biological Sciences, Beijing, China.
authors.forename: Huanwei
authors.initials: H
authors.lastname: Huang
authors.affiliation: National Institute of Biological Sciences, Beijing, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Cai
authors.affiliation: National Institute of Biological Sciences, Beijing, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D004330:Drosophila / Q000254:growth & development* / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D007814:Larva / Q000378:metabolism; D000076225:Mechanistic Target of Rapamycin Complex 2; D046912:Multiprotein Complexes / Q000235:genetics / Q000378:metabolism*; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D000076226:Rapamycin-Insensitive Companion of mTOR Protein; D058570:TOR Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D014921:Wings, Animal / Q000033:anatomy & histology / Q000502:physiology
chemical_list: D002352:Carrier Proteins; D029721:Drosophila Proteins; C578380:LST8 protein, Drosophila; D046912:Multiprotein Complexes; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D000076226:Rapamycin-Insensitive Companion of mTOR Protein; C496922:rictor protein, Drosophila; D000076225:Mechanistic Target of Rapamycin Complex 2; D058570:TOR Serine-Threonine Kinases