title: Phylogeny and expression pattern of starch branching enzyme family genes in cassava (Manihot esculenta Crantz) under diverse environments.
abstract: Starch branching enzyme (SBE) is one of the key enzymes involved in starch biosynthetic metabolism. In this study, six SBE family genes were identified from the cassava genome. Phylogenetic analysis divided the MeSBE family genes into dicot family A, B, C, and the new group. Tissue-specific analysis showed that MeSBE2.2 was strongly expressed in leaves, stems cortex, and root stele, and MeSBE3 had high expression levels in stem cortex and root stele of plants in the rapid growth stage under field condition, whereas the expression levels of MeSBE2.1, MeSBE4, and MeSBE5 were low except for in stems cortex. The transcriptional activity of MeSBE2.2 and MeSBE3 was higher compared with other members and gradually increased in the storage roots during root growth process, while the other MeSBE members normally remained low expression levels. Expression of MeSBE2.2 could be induced by salt, drought, exogenous abscisic acid, jasmonic acid, and salicylic acid signals, while MeSBE3 had positive response to drought, salt, exogenous abscisic acid, and salicylic acid in leaves but not in storage root, indicating that they might be more important in starch biosynthesis pathway under diverse environments.
authors.affiliation: College of Agriculture, Hainan University, Renmin Road No. 58, Haikou, 570228, People's Republic of China.
authors.forename: Jinli
authors.initials: J
authors.lastname: Pei
authors.forename: Huijun
authors.initials: H
authors.lastname: Wang
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Xia
authors.forename: Chen
authors.initials: C
authors.lastname: Liu
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.forename: Pingan
authors.initials: P
authors.lastname: Ma
authors.forename: Cheng
authors.initials: C
authors.lastname: Lu
authors.forename: Wenquan
authors.initials: W
authors.lastname: Wang
mesh_terms: D015061:1,4-alpha-Glucan Branching Enzyme / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D017124:Conserved Sequence; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D002365:Manihot / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D013312:Stress, Physiological
chemical_list: D010940:Plant Proteins; D015061:1,4-alpha-Glucan Branching Enzyme