title: Hydrogen Suppresses Hypoxia/Reoxygenation-Induced Cell Death in Hippocampal Neurons Through Reducing Oxidative Stress.
abstract: OBJECTIVE
  Deep hypothermic circulatory arrest (DHCA) is a cerebral protection technique that has been used in the operations involving the aortic arch and brain aneurysm for decades. We previous showed that DHCA treated rats developed a significant oxidative stress and apoptosis in neurons. We here intend to investigate the protective the effect of hydrogen against oxidative stress-induced cell injury and the involved mechanisms using an in vitro experimental model of hypoxia/reoxygenation (H/R) on HT-22 cells.
  
  
  METHODS
  The model of H/R was established using an airtight culture container and the anaeropack. Measurement of mitochondrial membrane potential (MMP) and reactive oxygen species (ROS) production was used H2DCFDA and JC-1 staining. Western blot was used for the quantification of Akt, p-Akt, Bcl-2, Bax and cleaved caspase-3 proteins. The microRNA (miRNA) profile in hippocampal neurons from rat model of DHCA was determined by miRNA deep sequencing.
  
  
  RESULTS
  The elevation of ROS and reduction of MMP were significantly induced by the treatment with hypoxia for 18 h followed by reoxygenation for 6 h. Hydrogen treatment significantly reduced H/R-caused cell death. The levels of p-Akt (Ser 473) and Bcl-2 were significantly increased while Bax and cleaved caspase-3 were decreased by hydrogen treatment on the model of H/R. The expression of miR-200 family was significantly elevated in model of DHCA and H/R. Hydrogen administration inhibited the H/R-induced expression of miR-200 family in HT-22 cells. In addition, inhibition of miR-200 family suppressed H/R-caused cell death through reducing ROS production.
  
  
  CONCLUSIONS
  These results suggest that H/R causes oxidative stress-induced cell death and that the hydrogen protects against H/R-induced cell death in HT22 cells, in part, due to reducing expression of miR-200 family.
authors.forename: Rong
authors.initials: R
authors.lastname: Wei
authors.forename: Rufang
authors.initials: R
authors.lastname: Zhang
authors.forename: Yewei
authors.initials: Y
authors.lastname: Xie
authors.forename: Li
authors.initials: L
authors.lastname: Shen
authors.forename: Fang
authors.initials: F
authors.lastname: Chen
mesh_terms: D000818:Animals; D016923:Cell Death; D015687:Cell Hypoxia; D002460:Cell Line; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D006859:Hydrogen / Q000627:therapeutic use*; D007036:Hypothermia, Induced / Q000009:adverse effects*; D000860:Hypoxia / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D008297:Male; D053078:Membrane Potential, Mitochondrial; D009474:Neurons / Q000378:metabolism / Q000473:pathology*; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D006859:Hydrogen; D010100:Oxygen