title: Role of rhIL-17 in regulating the mitochondrial pathway proteins in peripheral blood neutrophils.
abstract: BACKGROUND
  IL-17, classified as an inflammatory cytokine, plays a key role in the activation of inflammatory processes involving neutrophils.
  
  
  METHODS
  Twenty healthy voluntary blood donors were controlled in the study. The granulocyte suspensions were stimulated with rhIL-17 and fMLP. Expression of Bcl-xl, Smac/DIABLO, and Omi/HtrA2 in neutrophil lysates were assessed by Western blot. The level of cytochrome c and activity of caspase 9 was also assayed in these cells.
  
  
  RESULTS
  The results of existing research highlight the importance of rhIL-17 in reducing the survival of neutrophils via the mitochondria, depending on the Bcl-2 protein family. Our research has indicated that rhIL-17 regulates the mutual relationships between the proteins of that family. The proapoptotic effect observed in neutrophils affected by rhIL-17 is a result of a decreased expression of Bcl-xl. Consequently, the expression of apoptogenic proteins, including cytochrome c, Smac/DIABLO, and Omi/HtrA2, is elevated. Surprisingly, there have been no observations of the cytokine influencing the activity of caspase 9.
  
  
  CONCLUSIONS
  Results have shown for the first time that IL-17 has a direct effect on the decrease of Bcl-xl. In conclusion, the results of the research presented in this article confirm the dual action of IL-17, which, on the one hand, leads to an array of proinflammatory mechanisms regarding neutrophils and, on the other hand, reduces the survival of those cells via an immediate influence on the Bcl-2 family of proteins and apoptogenic factors.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Kakareko
authors.forename: Ewa
authors.initials: E
authors.lastname: Jabłońska
authors.forename: Wioletta
authors.initials: W
authors.lastname: Ratajczak-Wrona
mesh_terms: D000328:Adult; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins; D001782:Blood Donors; D002470:Cell Survival; D045304:Cytochromes c / Q000097:blood; D006098:Granulocytes / Q000378:metabolism; D064368:Healthy Volunteers; D000074923:High-Temperature Requirement A Serine Peptidase 2; D006801:Humans; D007249:Inflammation; D020381:Interleukin-17 / Q000097:blood*; D047908:Intracellular Signaling Peptides and Proteins / Q000097:blood; D008875:Middle Aged; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000097:blood; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism*; D012697:Serine Endopeptidases / Q000097:blood; D055815:Young Adult; D051020:bcl-X Protein / Q000097:blood*
chemical_list: D051017:Apoptosis Regulatory Proteins; C494811:BCL2L1 protein, human; C412403:DIABLO protein, human; D020381:Interleukin-17; D047908:Intracellular Signaling Peptides and Proteins; D024101:Mitochondrial Proteins; D051020:bcl-X Protein; D009240:N-Formylmethionine Leucyl-Phenylalanine; D045304:Cytochromes c; D012697:Serine Endopeptidases; C000615914:HTRA2 protein, human; D000074923:High-Temperature Requirement A Serine Peptidase 2