title: Characterisation of polyphenol constituents of Linderae aggregate leaves using HPLC fingerprint analysis and their antioxidant activities.
abstract: The leaves of Linderae aggregate (LAL) has been used as a type of tea in China and other Southeast Asian countries. In this study, 11 polyphenols in LAL were clarified for the first time using multiple high-performance liquid chromatographic techniques. An optimal extracting method was developed through the comparison of the amount of quercetin-3-O-α-L-rhamnoside using a uniform design method. From the fingerprint liquid chromatography data, 11 common peaks in the 8 samples collected from April to November were semi-determined. The antioxidant capacities were examined using the 2,2-diphenyl-1-picrylhydrazyl free radical scavenging assay and the ferric reducing/antioxidant power assay. All 8 samples contained the same 11 polyphenols in similar ratios. Three samples, S2, S5 and S6 contained higher amount of quercetin-3-O-α-L-rhamnoside and were demonstrated to have stronger antioxidant capacities in both antioxidant assays. These results are critical in optimising harvest time and quality control of LAL.
authors.affiliation: College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Chanjuan
authors.initials: C
authors.lastname: Xu
authors.affiliation: College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Bingxian
authors.initials: B
authors.lastname: Yang
authors.affiliation: College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Changshu Qiushi Technology Co. Ltd., Changshu, Jiangsu, China.
authors.forename: Ximin
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Jingkui
authors.initials: J
authors.lastname: Tian
authors.affiliation: College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou, Zhejiang, China. Electronic address: zhanglin@zju.edu.cn.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005419:Flavonoids / Q000737:chemistry; D027443:Lindera / Q000737:chemistry*; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D059808:Polyphenols / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010936:Plant Extracts; D059808:Polyphenols
keywords: Antioxidant assays; Flavonoid; Linderae aggregate; Polyphenol; Quality control