title: Impact of a phosphate fertilizer plant on the contamination of marine biota by heavy elements.
abstract: Due to their toxicity, persistence, and bioaccumulation, metals are important marine environment pollutants, especially in low renewal rate water such as the Mediterranean Sea, receiving a lot of untreated industrial waste. The impact of a phosphate fertilizer plant on the marine biota metal contamination was studied. Several types of organisms: crabs, mussels, patella and fish were collected from two areas of the Lebanese coast, one subjected to the impact of the plant and another away from it; samples were analyzed for Zn, U, Cr, V, Mn, Ni, Co, Cu, As, Cd and Pb by ICP-MS. Higher accumulation was in crabs, patella, and mussels. Fish accumulated principally Zn, Cu, and Cd; a difference was observed between species and tissues. Cytosol metal fractionation using size-exclusion LC-ICP-MS showed principally Pb, As, Co, and Mn in the low molecular weight fraction (<1.8 Da); Cd, Zn, and Cu in the metallothionein fraction (1.8--18 k Da), and Ni in high molecular weight fraction (>20 kDa).
authors.affiliation: Lebanese Atomic Energy Commission, National Council for Scientific Research, P.O. Box: 11-8281, Beirut, Lebanon. maria.aoun@cnrs.edu.lb.|Laboratoire de Chimie Analytique Bio-inorganique et Environnement, CNRS/UPPA, Hélioparc, Pau, France. maria.aoun@cnrs.edu.lb.
authors.forename: Maria
authors.initials: M
authors.lastname: Aoun
authors.affiliation: Laboratoire de Chimie Analytique Bio-inorganique et Environnement, CNRS/UPPA, Hélioparc, Pau, France.
authors.forename: Carine
authors.initials: C
authors.lastname: Arnaudguilhem
authors.affiliation: Lebanese Atomic Energy Commission, National Council for Scientific Research, P.O. Box: 11-8281, Beirut, Lebanon.
authors.forename: Omar
authors.initials: O
authors.lastname: El Samad
authors.affiliation: Lebanese Atomic Energy Commission, National Council for Scientific Research, P.O. Box: 11-8281, Beirut, Lebanon.
authors.forename: Rola Bou
authors.initials: RB
authors.lastname: Khozam
authors.affiliation: Laboratoire de Chimie Analytique Bio-inorganique et Environnement, CNRS/UPPA, Hélioparc, Pau, France.
authors.forename: Ryszard
authors.initials: R
authors.lastname: Lobinski
mesh_terms: D000818:Animals; D059001:Aquatic Organisms / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D058448:Biota / Q000187:drug effects*; D005308:Fertilizers; D019015:Geologic Sediments / Q000737:chemistry; D007220:Industrial Waste / Q000032:analysis; D008522:Mediterranean Sea; D019216:Metals, Heavy / Q000378:metabolism*; D010710:Phosphates / Q000494:pharmacology*; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D005308:Fertilizers; D007220:Industrial Waste; D019216:Metals, Heavy; D010710:Phosphates; D014874:Water Pollutants, Chemical
keywords: Bioaccumulation; Biota; Heavy metals; LC-ICP-MS; Metal fractionation; Size exclusion