title: Intestinal amebae.
abstract: Among the Entamoeba species that infect humans, Entamoeba histolytica causes diseases, Entamoeba dispar is a harmless commensal, Entamoeba moshkovskii seems to be a pathogen, and the pathogenicity of Entamoeba bangladeshi remains to be investigated. Species-specific detection needed for treatment decisions and for understanding the epidemiology and pathogenicity of these amebae. Antigen-based detection methods are needed for E dispar, E moshkovskii, and E bangladeshi; and molecular diagnostic test capable of detecting E histolytica, E dispar, E moshkovskii, and E bangladeshi simultaneously in clinical samples. Next-generation sequencing of DNA from stool is needed to identify novel species of Entamoeba.
authors.affiliation: Division of Foodborne Waterborne and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road Northeast, Mailstop D-66, Atlanta, GA 30329, USA. Electronic address: xzn5@cdc.gov.
authors.forename: Ibne Karim M
authors.initials: IK
authors.lastname: Ali
mesh_terms: D000953:Antigens, Protozoan / Q000032:analysis; D004747:Entamoeba / Q000145:classification* / Q000302:isolation & purification / Q000472:pathogenicity; D004749:Entamoebiasis / Q000175:diagnosis*; D005243:Feces / Q000469:parasitology; D006801:Humans; D000067716:Point-of-Care Testing; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods; D013045:Species Specificity; D013048:Specimen Handling
chemical_list: D000953:Antigens, Protozoan
keywords: Amebiasis laboratory diagnosis; Antigen detection; Entamoeba Bangladeshi; Entamoeba dispar; Entamoeba histolytica; Entamoeba moshkovskii; Molecular detection; Serology