title: Accurate flow cytometric gating of the large lymphocyte region is a powerful screening method for detecting hairy cell leukemia presenting with a low tumor burden.
abstract: Hairy cell leukemia typically presents with pancytopenia and often mimics aplastic anemia. Making an accurate diagnosis is crucial, as treatment with the purine analogues cladribine and pentostatin brings about durable complete remission in the majority of patients. Surface kappa and lambda flow cytometric analyses of peripheral blood or bone marrow are a powerful screening tool, although routine gating of the entire lymphocyte region may fail to show light chain restriction due to a low tumor burden. We herein demonstrate that accurate subgating of the large lymphocyte region is essential and recommend the application of this method in all cases of pancytopenia of unknown etiology.
authors.affiliation: Division of Hematology, Department of Medicine, Juntendo University School of Medicine, Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Yasuda
authors.forename: Miyuki
authors.initials: M
authors.lastname: Tsutsui
authors.forename: Masaru
authors.initials: M
authors.lastname: Tanaka
authors.forename: Marito
authors.initials: M
authors.lastname: Araki
authors.forename: Soji
authors.initials: S
authors.lastname: Morishita
authors.forename: Makoto
authors.initials: M
authors.lastname: Sasaki
authors.forename: Akimichi
authors.initials: A
authors.lastname: Ohsaka
authors.forename: Norio
authors.initials: N
authors.lastname: Komatsu
mesh_terms: D000741:Anemia, Aplastic / Q000175:diagnosis; D000970:Antineoplastic Agents / Q000627:therapeutic use; D001853:Bone Marrow / Q000473:pathology; D003937:Diagnosis, Differential; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007943:Leukemia, Hairy Cell / Q000175:diagnosis* / Q000188:drug therapy / Q000473:pathology*; D008214:Lymphocytes / Q000473:pathology; D008297:Male; D008875:Middle Aged; D010198:Pancytopenia / Q000175:diagnosis; D047368:Tumor Burden
chemical_list: D000970:Antineoplastic Agents