title: Structural and functional studies of membrane remodeling machines.
abstract: Building cells from their component parts will hinge upon our ability to reconstitute biochemical compartmentalization and exchange between membrane-delimited organelles. By contrast with our understanding of other cellular events, the mechanisms that govern membrane trafficking has lagged because the presence of phospholipid bilayers complicates the use of standard methods. This chapter describes in vitro methods for purifying, reconstituting, and visualizing membrane remodeling activities directly by electron cryomicroscopy.
authors.affiliation: Department of Biochemistry, University of Utah, School of Medicine, Salt Lake City, UT, USA; Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA, USA.
authors.forename: Raghav
authors.initials: R
authors.lastname: Kalia
authors.affiliation: Department of Biochemistry, University of Utah, School of Medicine, Salt Lake City, UT, USA; Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA, USA.
authors.forename: Nathaniel
authors.initials: N
authors.lastname: Talledge
authors.affiliation: Department of Biochemistry, University of Utah, School of Medicine, Salt Lake City, UT, USA; Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA, USA.
authors.forename: Adam
authors.initials: A
authors.lastname: Frost
mesh_terms: D000217:Acyltransferases / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D020285:Cryoelectron Microscopy / Q000379:methods*; D034281:Dynamins / Q000096:biosynthesis / Q000378:metabolism*; D004705:Endocytosis / Q000502:physiology; D004926:Escherichia coli / Q000378:metabolism; D008051:Lipid Bilayers; D017434:Protein Structure, Tertiary; D061987:Sf9 Cells; D018411:Spodoptera
chemical_list: D008051:Lipid Bilayers; D000217:Acyltransferases; C018764:2-acylglycerophosphate acyltransferase; D034281:Dynamins
keywords: Bilayer; Dynamin; Electron cryomicroscopy; Endocytosis; Endophilin; Lipid-binding; Membrane remodeling; Mitochondria; cryo-EM