title: Raphidascaris (Raphidascaris) macrouri n. sp. (Nematoda: Anisakidae) from two deep-sea macrourid fishes in the Western Mediterranean: Morphological and molecular characterisations.
abstract: A new nematode species, Raphidascaris (Raphidascaris) macrouri n. sp. (Anisakidae), is described from male and female specimens found in the intestine, and occasionally in stomach and pyloric caeca, of two deep-water macrourid fishes (Gadiformes) off Barcelona, Mediterranean Sea: Nezumia aequalis (Günther) (type-host) and Trachyrincus scabrus (Rafinesque). Based on light and scanning electron microscopy examination, the new species shows similar morphological features as the other four valid species of the subgenus Raphidascaris Railliet & Henry, 1915, but it differs from Raphidascaris (Raphidascaris) acus (Bloch, 1779), Raphidascaris (Raphidascaris) lutjani Olsen, 1952 and Raphidascaris (Raphidascaris) mediterraneus Lèbre & Petter, 1983 in the high number of precloacal papillae (23-32) and from Raphidascaris (Raphidascaris) gigi Fujita, 1928 in the length of the spicules. Moreover, Raphidascaris (Raphidascaris) macrouri n. sp. exhibits a high variability on the number and distribution of caudal papillae, which was not recorded in the other four mentioned species. This is the first species of this subgenus reported from the family Macrouridae. Sequences of ITS1-5.8S-ITS2 region are analysed and compared with closely related nematode species. Molecular analysis confirmed the uniformity of the R. (R.) macrouri n. sp. between hosts.
authors.affiliation: Departament de Biologia Animal, Biologia Vegetal i Ecologia, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, 08193 Barcelona, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Pérez-i-García
authors.affiliation: Departament de Biologia Animal, Biologia Vegetal i Ecologia, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, 08193 Barcelona, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Constenla
authors.affiliation: Departament de Biologia Animal, Biologia Vegetal i Ecologia, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, 08193 Barcelona, Spain. Electronic address: Maite.Carrasson@uab.cat.
authors.forename: Maite
authors.initials: M
authors.lastname: Carrassón
authors.affiliation: Institut Cavanilles de Biodiversitat i Biologia Evolutiva, Parc Científic, Universitat de València, PO Box 22085, 46071 Valencia, Spain.
authors.forename: Francisco E
authors.initials: FE
authors.lastname: Montero
authors.affiliation: Departament de Biologia Animal, Biologia Vegetal i Ecologia, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, 08193 Barcelona, Spain.
authors.forename: Anna
authors.initials: A
authors.lastname: Soler-Membrives
authors.affiliation: Laboratorio de Necton, El Colegio de la Frontera Sur (ECOSUR), Unidad Chetumal, Chetumal, Quintana Roo, Mexico; Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Branišovska 31, 370 05 České Budějovice, Czech Republic.
authors.forename: David
authors.initials: D
authors.lastname: González-Solís
mesh_terms: D000818:Animals; D005260:Female; D005393:Fish Diseases / Q000453:epidemiology / Q000469:parasitology*; D005399:Fishes; D008297:Male; D008522:Mediterranean Sea / Q000453:epidemiology; D009348:Nematoda / Q000145:classification* / Q000648:ultrastructure*; D009349:Nematode Infections / Q000662:veterinary*; D013045:Species Specificity
keywords: Deep-water; Macrouridae; Nezumia aequalis; Raphidascaris (Raphidascaris) macrouri; Spain; Trachyrincus scabrus