title: Recombinogenic activity of Pantoprazole(®) in somatic cells of Drosophila melanogaster.
abstract: Pantoprazole(®) is one of the leading proton pump inhibitors (PPIs) used in the treatment of a variety of diseases related to the upper gastrointestinal tract. However, studies have shown an increased risk of developing gastric cancer, intestinal metaplasia and hyperplasia of endocrine cells with prolonged use. In the present study, the somatic mutation and recombination test (SMART) was employed to determine the mutagenic effects of Pantoprazole on Drosophila melanogaster. Repeated treatments with Pantoprazole were performed on 72-hour larvae of the standard (ST) and high bioactivation (HB) crosses at concentrations of 2.5, 5.0, and 10.0 μM. In addition, doxorubicin (DXR) was administered at 0.4 mM, as a positive control. When administered to ST descendants, total number of spots were statistically significant at 2.5 and 5.0 μM concentrations. For HB descendants, a significant increase in the total number of spots was observed among the marked transheterozygous (MH) flies. Through analysis of balancer heterozygous (BH) descendants, recombinogenic effects were observed at all concentrations in descendants of the HB cross. In view of these experimental conditions and results, it was concluded that Pantoprazole is associated with recombinogenic effects in Drosophila melanogaster.
authors.affiliation: Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Campus Umuarama, Uberlândia, MG, Brazil .
authors.forename: Jeyson Césary
authors.initials: JC
authors.lastname: Lopes
authors.affiliation: Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Campus Umuarama, Uberlândia, MG, Brazil .
authors.forename: Nayane Moreira
authors.initials: NM
authors.lastname: Machado
authors.affiliation: Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Campus Umuarama, Uberlândia, MG, Brazil .
authors.forename: Rosiane Soares
authors.initials: RS
authors.lastname: Saturnino
authors.affiliation: Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Campus Umuarama, Uberlândia, MG, Brazil . ; Laboratório de Citogenética e Mutagênese, Centro Universitário de Patos de Minas, Patos de Minas, MG, Brazil .
authors.forename: Júlio César
authors.initials: JC
authors.lastname: Nepomuceno
keywords: Drosophila melanogaster; Pantoprazole; SMART; mutagenicity; proton pump inhibitors; recombinogenicity