title: Lipid nanoparticles as an emerging platform for cannabinoid delivery: physicochemical optimization and biocompatibility.
abstract: This work aims at developing and optimizing a valuable oral delivery carrier for the cannabinoid derivative CB13, which presents a high therapeutic potential in chronic pain states that respond poorly to conventional analgesics, but also shows highly unfavorable physicochemical properties. CB13-loaded lipid nanoparticles (LNP) formulations were developed through solvent-emulsion evaporation and optimized in terms of physicochemical properties, long-term stability, integrity under gastric simulated conditions and in vitro interaction with NIH 3T3, HEK 293T and Caco-2 cells. An optimized formulation of LNP containing CB13 was obtained from a wide range of conditions assayed and analyzed. The selection of the lipid core, production conditions and the inclusion of lecithin proved to be key factors for the final properties of encapsulation, integrity and performance of the carriers. The LNP formulation proposed proved to be a promising carrier for the oral delivery of CB13, a cannabinoid with high therapeutic potential in chronic pain states that currently lack a valid oral treatment.
authors.affiliation: a Facultad de Farmacia, Universidad de Sevilla, Profesor García González , Sevilla , Spain and.|b Research Institute for Medicines and Pharmaceutical Sciences (iMed.UL), Faculdade de Farmácia da Universidade de Lisboa , Lisboa , Portugal.
authors.forename: M
authors.initials: M
authors.lastname: Durán-Lobato
authors.affiliation: a Facultad de Farmacia, Universidad de Sevilla, Profesor García González , Sevilla , Spain and.
authors.forename: L
authors.initials: L
authors.lastname: Martín-Banderas
authors.affiliation: b Research Institute for Medicines and Pharmaceutical Sciences (iMed.UL), Faculdade de Farmácia da Universidade de Lisboa , Lisboa , Portugal.
authors.forename: R
authors.initials: R
authors.lastname: Lopes
authors.affiliation: b Research Institute for Medicines and Pharmaceutical Sciences (iMed.UL), Faculdade de Farmácia da Universidade de Lisboa , Lisboa , Portugal.
authors.forename: L M D
authors.initials: LM
authors.lastname: Gonçalves
authors.affiliation: a Facultad de Farmacia, Universidad de Sevilla, Profesor García González , Sevilla , Spain and.
authors.forename: M
authors.initials: M
authors.lastname: Fernández-Arévalo
authors.affiliation: b Research Institute for Medicines and Pharmaceutical Sciences (iMed.UL), Faculdade de Farmácia da Universidade de Lisboa , Lisboa , Portugal.
authors.forename: A J
authors.initials: AJ
authors.lastname: Almeida
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002186:Cannabinoids / Q000008:administration & dosage / Q000737:chemistry; D002626:Chemistry, Pharmaceutical / Q000379:methods; D004337:Drug Carriers / Q000737:chemistry*; D004339:Drug Compounding / Q000379:methods; D016503:Drug Delivery Systems; D004355:Drug Stability; D057809:HEK293 Cells; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D051379:Mice; D041681:NIH 3T3 Cells; D053758:Nanoparticles; D009281:Naphthalenes / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D002186:Cannabinoids; D004337:Drug Carriers; D008055:Lipids; D009281:Naphthalenes; C524107:naphthalen-1-yl-(4-pentyloxynaphthalen-1-yl)methanone
keywords: Lipid carriers; nanoparticle formulation; neuropathic pain; oral delivery; poor soluble drugs