title: In vivo single-cell labeling by confined primed conversion.
abstract: Spatially confined green-to-red photoconversion of fluorescent proteins with high-power, pulsed laser illumination is negligible, thus precluding optical selection of single cells in vivo. We report primed conversion, in which low-power, dual-wavelength, continuous-wave illumination results in pronounced photoconversion. With a straightforward addition to a conventional confocal microscope, we show confined primed conversion in living zebrafish and reveal the complex anatomy of individual neurons packed between neighboring cells.
authors.affiliation: Department of Biosystems Science and Engineering (D-BSSE), ETH Zurich, Basel, Switzerland.
authors.forename: William P
authors.initials: WP
authors.lastname: Dempsey
authors.affiliation: Department of Biosystems Science and Engineering (D-BSSE), ETH Zurich, Basel, Switzerland.
authors.forename: Lada
authors.initials: L
authors.lastname: Georgieva
authors.affiliation: Department of Biosystems Science and Engineering (D-BSSE), ETH Zurich, Basel, Switzerland.
authors.forename: Patrick M
authors.initials: PM
authors.lastname: Helbling
authors.affiliation: Department of Biosystems Science and Engineering (D-BSSE), ETH Zurich, Basel, Switzerland.
authors.forename: Ali Y
authors.initials: AY
authors.lastname: Sonay
authors.affiliation: Department of Molecular and Computational Biology, University of Southern California, Los Angeles, California, USA.
authors.forename: Thai V
authors.initials: TV
authors.lastname: Truong
authors.affiliation: Department of Biosystems Science and Engineering (D-BSSE), ETH Zurich, Basel, Switzerland.
authors.forename: Michel
authors.initials: M
authors.lastname: Haffner
authors.affiliation: Department of Biosystems Science and Engineering (D-BSSE), ETH Zurich, Basel, Switzerland.
authors.forename: Periklis
authors.identifier: 0000000283679332
authors.initials: P
authors.lastname: Pantazis
mesh_terms: D000818:Animals; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D009474:Neurons / Q000166:cytology*; D015027:Zebrafish