title: Photo-triggered fluorescent labelling of recombinant proteins in live cells.
abstract: A method to photo-chemically trigger fluorescent labelling of proteins in live cells is developed. The approach is based on photo-caged split-intein mediated conditional protein trans-splicing reaction and enabled background-free fluorescent labelling of target proteins with the necessary spatiotemporal control.
authors.affiliation: Department of Biomedical Engineering, Dongguk University-Seoul, Pildong 3-ga, Seoul, Korea. ykwon@dongguk.edu.
authors.forename: Deokho
authors.initials: D
authors.lastname: Jung
authors.forename: Kohei
authors.initials: K
authors.lastname: Sato
authors.forename: Kyoungmi
authors.initials: K
authors.lastname: Min
authors.forename: Akira
authors.initials: A
authors.lastname: Shigenaga
authors.forename: Juyeon
authors.initials: J
authors.lastname: Jung
authors.forename: Akira
authors.initials: A
authors.lastname: Otaka
authors.forename: Youngeun
authors.initials: Y
authors.lastname: Kwon
mesh_terms: D005453:Fluorescence; D006367:HeLa Cells; D006801:Humans; D047668:Inteins / Q000502:physiology*; D008027:Light; D058619:Maltose-Binding Proteins / Q000378:metabolism*; D015202:Protein Engineering; D019154:Protein Splicing / Q000502:physiology*; D011994:Recombinant Proteins / Q000737:chemistry* / Q000378:metabolism* / Q000528:radiation effects
chemical_list: D058619:Maltose-Binding Proteins; D011994:Recombinant Proteins