title: Second generation physical and linkage maps of yellowtail (Seriola quinqueradiata) and comparison of synteny with four model fish.
abstract: BACKGROUND
  Physical and linkage maps are important aids for the assembly of genome sequences, comparative analyses of synteny, and to search for candidate genes by quantitative trait locus analysis. Yellowtail, Seriola quinqueradiata, is an economically important species in Japanese aquaculture, and genetic information will be useful for DNA-assisted breeding. We report the construction of a second generation radiation hybrid map, its synteny analysis, and a second generation linkage map containing SNPs (single nucleotide polymorphisms) in yellowtail.
  
  
  RESULTS
  Approximately 1.4 million reads were obtained from transcriptome sequence analysis derived from 11 tissues of one individual. To identify SNPs, cDNA libraries were generated from a pool of 500 whole juveniles, and the gills and kidneys of 100 adults. 9,356 putative SNPs were detected in 6,025 contigs, with a minor allele frequency ≥ 25%. The linkage and radiation hybrid maps were constructed based on these contig sequences. 2,081 markers, including 601 SNPs markers, were mapped onto the linkage map, and 1,532 markers were mapped in the radiation hybrid map.
  
  
  CONCLUSIONS
  The second generation linkage and physical maps were constructed using 6,025 contigs having SNP markers. These maps will aid the de novo assembly of sequencing reads, linkage studies and the identification of candidate genes related to important traits. The comparison of marker contigs in the radiation hybrid map indicated that yellowtail is evolutionarily closer to medaka than to green-spotted pufferfish, three-spined stickleback or zebrafish. The synteny analysis may aid studies of chromosomal evolution in yellowtail compared with model fish.
authors.affiliation: National Research Institute of Aquaculture, Fisheries Research Agency, 224-1 Hiruta, Tamaki-cho, Watarai-gun, Mie, 519-0423, Japan. junaoki@affrc.go.jp.
authors.forename: Jun-ya
authors.initials: JY
authors.lastname: Aoki
authors.affiliation: National Research Institute of Aquaculture, Fisheries Research Agency, 224-1 Hiruta, Tamaki-cho, Watarai-gun, Mie, 519-0423, Japan. wataru.kai@fluidigm.com.
authors.forename: Wataru
authors.initials: W
authors.lastname: Kai
authors.affiliation: National Research Institute of Aquaculture, Fisheries Research Agency, 224-1 Hiruta, Tamaki-cho, Watarai-gun, Mie, 519-0423, Japan. yumi_8674@hotmail.com.
authors.forename: Yumi
authors.initials: Y
authors.lastname: Kawabata
authors.affiliation: National Research Institute of Aquaculture, Fisheries Research Agency, 422-1 Nakatsuhamaura, Minamiise-cho, Watarai-gun, Mie, 516-0193, Japan. aozaki@affrc.go.jp.
authors.forename: Akiyuki
authors.initials: A
authors.lastname: Ozaki
authors.affiliation: Goto Laboratory, Seikai National Fisheries Research Institute, Fisheries Research Agency, 122-7, Nunoura, Tamanoura-cho, Goto, Nagasaki, 853-0508, Japan. kyosida@affrc.go.jp.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Yoshida
authors.affiliation: Faculty of Marine Science, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo, 108-8477, Japan. takashi_k1201@hotmail.com.
authors.forename: Takashi
authors.initials: T
authors.lastname: Koyama
authors.affiliation: Faculty of Marine Science, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo, 108-8477, Japan. takashis@kaiyodai.ac.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Sakamoto
authors.affiliation: National Research Institute of Aquaculture, Fisheries Research Agency, 224-1 Hiruta, Tamaki-cho, Watarai-gun, Mie, 519-0423, Japan. arakin@affrc.go.jp.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Araki
mesh_terms: D000818:Animals; D019143:Evolution, Molecular; D020869:Gene Expression Profiling; D008040:Genetic Linkage; D016678:Genome; D023421:Models, Animal; D009990:Oryzias / Q000235:genetics*; D010473:Perciformes / Q000235:genetics*; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide; D021101:Radiation Hybrid Mapping / Q000379:methods*; D026801:Synteny; D023721:Tetraodontiformes / Q000235:genetics*; D015027:Zebrafish / Q000235:genetics*