title: [Methylation Status of miR-182 Promoter in Lung Cancer Cell Lines].
abstract: OBJECTIVE
  It has been proven that the abnormal expression of miR-182 was related to the occurrence and development of tumors. The aim of this study is to explore the relationship between the methylation of miR-182 promoter and its expression in lung cancer cell lines.
  
  
  METHODS
  Real-time quantitative PCR and methylation-specific PCR were used to detect the expression level of miR-182 and its promoter methylation status in five lung cancer cell lines (A549, L9981, NL9980, 95C and 95D). DNA sequencing was used to confirm the methylation results.
  
  
  RESULTS
  The level of miR-182 expression significantly differs among these lung cancer cell lines. The highly metastatic human lung cancer cell lines, namely, A549 and L9981, demonstrate a relatively lower expression level of miR-182 compared with the lowly metastatic human lung cancer cell line 95C. Methylation-specific PCR and DNA sequencing assay results indicate that these lung cancer cell lines present different levels of miR-182 promoter methylation, and the highest methylation level is observed in A549 cells. Furthermore, the expression of miR-182 in these cell lines significantly increases when treated with 10 μM 5'-Aza-dC.
  
  
  CONCLUSIONS
  DNA methylation occurs in the miR-182 promoter region in lung cancer cell lines. This methylation can regulate the expression level of miR-182. Further study must be conducted to explore the function of miR-182 promoter methylation in lung cancer occurrence and development. .
authors.affiliation: Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital,  Tianjin 300052, China.
authors.forename: Yongwen
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Lung Cancer Surgery, Tianjin Medical University General Hospital,  Tianjin 300052, China.
authors.forename: Yonglin
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Lung Cancer Surgery, Tianjin Medical University General Hospital,  Tianjin 300052, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Ren
authors.affiliation: Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital,  Tianjin 300052, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Lung Cancer Surgery, Tianjin Medical University General Hospital,  Tianjin 300052, China.
authors.forename: Minghui
authors.initials: M
authors.lastname: Liu
authors.affiliation: Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital,  Tianjin 300052, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Liu
authors.affiliation: Tianjin Lung Cancer Institute;Department of Lung Cancer Surgery, Tianjin Medical University General Hospital,  Tianjin 300052, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
mesh_terms: D045744:Cell Line, Tumor; D019175:DNA Methylation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic
chemical_list: D035683:MicroRNAs; C538984:Mirn182 microRNA, human