title: Winogradskyella eckloniae sp. nov., a marine bacterium isolated from the brown alga Ecklonia cava.
abstract: A novel bacterial strain, designated EC29(T), was isolated from the brown alga Ecklonia cava collected on Jeju Island, Republic of Korea. Cells of strain EC29(T) were Gram-stain-negative, aerobic, rod-shaped and motile by gliding. Growth was observed at 10-30 °C (optimum, 20-25 °C), at pH 6.0-9.5 (optimum, pH 7.5) and in the presence of 1-5% (w/v) NaCl. Phylogenetic analyses based on the 16S rRNA gene sequence revealed that the strain belonged to the genus Winogradskyella. Strain EC29(T) exhibited the highest 16S rRNA gene sequence similarities, of 96.5-97.8%, to the type strains of Winogradskyella pulchriflava EM106(T), Winogradskyella echinorum KMM 6211(T) and Winogradskyella ulvae KMM 6390(T). Strain EC29(T) exhibited < 27% DNA-DNA relatedness with Winogradskyella pulchriflava EM106(T) and Winogradskyella echinorum KMM 6211(T). The predominant fatty acids of strain EC29(T) were iso-C15 : 0, iso-C15 : 1 G, C15 : 0, iso-C17 : 0 3-OH, iso-C15 : 0 3-OH and anteiso-C15 : 0. The DNA G+C content was 31.1 mol% and the major respiratory quinone was menaquinone-6 (MK-6). Based on a polyphasic study, strain EC29(T) is considered to represent a novel species of the genus Winogradskyella, for which the name Winogradskyella eckloniae sp. nov. is proposed. The type strain is EC29(T) ( = KCTC 32172(T) = JCM 18703(T)).
authors.affiliation: Department of Biology, Jeju National University, Jeju 690-756, Republic of Korea.|Jeju Biological Resource Co., Ltd, CTC Business Incubator, Jeju Tourism College, Jeju 690-756, Republic of Korea.
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Kim
authors.affiliation: Department of Aquatic Life Medicine, College of Ocean Science, Jeju 690-756, Republic of Korea.
authors.forename: So-Hyun
authors.initials: SH
authors.lastname: Park
authors.affiliation: Department of Biology, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Ga-Young
authors.initials: GY
authors.lastname: Seo
authors.affiliation: Jeju Biological Resource Co., Ltd, CTC Business Incubator, Jeju Tourism College, Jeju 690-756, Republic of Korea.
authors.forename: Young-Ju
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: Department of Biology, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Duck-Chul
authors.initials: DC
authors.lastname: Oh
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D000459:Phaeophyceae / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C042455:menaquinone 6