title: On chip analysis of CNS lymphoma in cerebrospinal fluid.
abstract: Molecular profiling of central nervous system lymphomas in cerebrospinal fluid (CSF) samples can be challenging due to the paucicellular and limited nature of the samples. Presented herein is a microfluidic platform for complete CSF lymphoid cell analysis, including single cell capture in sub-nanoliter traps, and molecular and chemotherapeutic response profiling via on-chip imaging, all in less than one hour. The system can detect scant lymphoma cells and quantitate their kappa/lambda immunoglobulin light chain restriction patterns. The approach can be further customized for measurement of additional biomarkers, such as those for differential diagnosis of lymphoma subtypes or for prognosis, as well as for imaging exposure to experimental drugs.
authors.affiliation: 1. Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA 02114, USA.
authors.forename: Anna
authors.initials: A
authors.lastname: Turetsky
authors.affiliation: 1. Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA 02114, USA.
authors.forename: Kyungheon
authors.initials: K
authors.lastname: Lee
authors.affiliation: 1. Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA 02114, USA ; 2. School of Engineering and Applied Sciences, Harvard University, Cambridge, MA.
authors.forename: Jun
authors.initials: J
authors.lastname: Song
authors.affiliation: 1. Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA 02114, USA.
authors.forename: Randy J
authors.initials: RJ
authors.lastname: Giedt
authors.affiliation: 1. Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA 02114, USA.
authors.forename: Eunha
authors.initials: E
authors.lastname: Kim
authors.affiliation: 3. Department of Pathology, Massachusetts General Hospital, Boston, MA 02114, USA.
authors.forename: Alexandra E
authors.initials: AE
authors.lastname: Kovach
authors.affiliation: 4. Cancer Center, Massachusetts General Hospital, Boston, MA 02114, USA.
authors.forename: Ephraim P
authors.initials: EP
authors.lastname: Hochberg
authors.affiliation: 1. Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA 02114, USA ; 4. Cancer Center, Massachusetts General Hospital, Boston, MA 02114, USA.
authors.forename: Cesar M
authors.initials: CM
authors.lastname: Castro
authors.affiliation: 1. Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA 02114, USA.
authors.forename: Hakho
authors.initials: H
authors.lastname: Lee
authors.affiliation: 1. Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA 02114, USA ; 5. Department of Systems Biology, Harvard Medical School, 200 Longwood Ave, Boston, MA 02115, USA.
authors.forename: Ralph
authors.initials: R
authors.lastname: Weissleder
mesh_terms: D016543:Central Nervous System Neoplasms / Q000175:diagnosis* / Q000473:pathology; D002555:Cerebrospinal Fluid / Q000166:cytology*; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D006801:Humans; D008223:Lymphoma / Q000175:diagnosis* / Q000473:pathology; D044085:Microfluidics / Q000379:methods*
keywords: cerebrospinal fluid; drug testing; lymphoma; microfluidics; point-of-care