title: Normal Taste Acceptance and Preference of PANX1 Knockout Mice.
abstract: Taste compounds detected by G protein-coupled receptors on the apical surface of Type 2 taste cells initiate an intracellular molecular cascade culminating in the release of ATP. It has been suggested that this ATP release is accomplished by pannexin 1 (PANX1). However, we report here that PANX1 knockout mice do not differ from wild-type controls in response to representative taste solutions, measured using 5-s brief-access tests or 48-h two-bottle choice tests. This implies that PANX1 is unnecessary for taste detection and consequently that ATP release from Type 2 taste cells does not require PANX1.
authors.affiliation: Monell Chemical Senses Center, 3500 Market Street, Philadelphia, PA 19104, USA, tordoff@monell.org.
authors.forename: Michael G
authors.initials: MG
authors.lastname: Tordoff
authors.affiliation: Monell Chemical Senses Center, 3500 Market Street, Philadelphia, PA 19104, USA.
authors.forename: Tiffany R
authors.initials: TR
authors.lastname: Aleman
authors.affiliation: Monell Chemical Senses Center, 3500 Market Street, Philadelphia, PA 19104, USA.
authors.forename: Hillary T
authors.initials: HT
authors.lastname: Ellis
authors.affiliation: Monell Chemical Senses Center, 3500 Market Street, Philadelphia, PA 19104, USA.
authors.forename: Makoto
authors.initials: M
authors.lastname: Ohmoto
authors.affiliation: Monell Chemical Senses Center, 3500 Market Street, Philadelphia, PA 19104, USA.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Matsumoto
authors.affiliation: Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami School of Medicine, Miami, FL 33136, USA.
authors.forename: Val I
authors.initials: VI
authors.lastname: Shestopalov
authors.affiliation: Department of Anatomy and Cell Biology, University of Pennsylvania, 240 South 40th Street, PA 19104, USA, and.
authors.forename: Claire H
authors.initials: CH
authors.lastname: Mitchell
authors.affiliation: Department of Physiology, University of Pennsylvania, 415 Curie Boulevard, Philadelphia, PA 19104, USA.
authors.forename: J Kevin
authors.initials: JK
authors.lastname: Foskett
authors.affiliation: Monell Chemical Senses Center, 3500 Market Street, Philadelphia, PA 19104, USA.
authors.forename: Rachel L
authors.initials: RL
authors.lastname: Poole
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D017630:Connexins / Q000032:analysis / Q000172:deficiency* / Q000378:metabolism*; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000172:deficiency* / Q000378:metabolism*; D013649:Taste / Q000502:physiology*; D055697:Taste Perception / Q000502:physiology
chemical_list: D017630:Connexins; D009419:Nerve Tissue Proteins; C503631:Panx1 protein, mouse; D000255:Adenosine Triphosphate
keywords: ATP release; CALHM1; gustometer; pannexin 1; two-bottle choice test