title: Enzyme-activated intracellular drug delivery with tubule clay nanoformulation.
abstract: Fabrication of stimuli-triggered drug delivery vehicle s is an important milestone in treating cancer. Here we demonstrate the selective anticancer drug delivery into human cells with biocompatible 50-nm diameter halloysite nanotube carriers. Physically-adsorbed dextrin end stoppers secure the intercellular release of brilliant green. Drug-loaded nanotubes penetrate through the cellular membranes and their uptake efficiency depends on the cells growth rate. Intercellular glycosyl hydrolases-mediated decomposition of the dextrin tube-end stoppers triggers the release of the lumen-loaded brilliant green, which allowed for preferable elimination of human lung carcinoma cells (А549) as compared with hepatoma cells (Hep3b). The enzyme-activated intracellular delivery of brilliant green using dextrin-coated halloysite nanotubes is a promising platform for anticancer treatment.
authors.affiliation: Bionanotechnology Lab, Institute of Fundamental Medicine and Biology, Kazan Federal University, Kreml uramı 18, Kazan, Republic of Tatarstan, Russian Federation, 420008.
authors.forename: Maria R
authors.initials: MR
authors.lastname: Dzamukova
authors.affiliation: Bionanotechnology Lab, Institute of Fundamental Medicine and Biology, Kazan Federal University, Kreml uramı 18, Kazan, Republic of Tatarstan, Russian Federation, 420008.
authors.forename: Ekaterina A
authors.initials: EA
authors.lastname: Naumenko
authors.affiliation: Institute for Micromanufacturing, Louisiana Tech University, 911 Hergot Ave., Ruston, LA, 71272, USA.
authors.forename: Yuri M
authors.initials: YM
authors.lastname: Lvov
authors.affiliation: 1] Bionanotechnology Lab, Institute of Fundamental Medicine and Biology, Kazan Federal University, Kreml uramı 18, Kazan, Republic of Tatarstan, Russian Federation, 420008 [2] Institute for Micromanufacturing, Louisiana Tech University, 911 Hergot Ave., Ruston, LA, 71272, USA.
authors.forename: Rawil F
authors.initials: RF
authors.lastname: Fakhrullin
mesh_terms: D000199:Actins / Q000737:chemistry; D000538:Aluminum Silicates / Q000737:chemistry; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D000077215:Clay; D003912:Dextrins / Q000737:chemistry; D004337:Drug Carriers / Q000494:pharmacology*; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy*; D008175:Lung Neoplasms / Q000188:drug therapy*; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D008928:Mitochondria / Q000187:drug effects; D043942:Nanotubes / Q000737:chemistry*; D004364:Pharmaceutical Preparations; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*
chemical_list: D000199:Actins; D000538:Aluminum Silicates; D003912:Dextrins; D004337:Drug Carriers; D004364:Pharmaceutical Preparations; D000644:Quaternary Ammonium Compounds; C006798:brilliant green; D000077215:Clay