title: In vitro effects induced by diesel exhaust at an air-liquid interface in a human lung alveolar carcinoma cell line A549.
abstract: The present study examined the effects induced in vitro in human adenocarcinoma-derived alveolar basal epithelial A549 cells by diesel particulate matter (DPM) administered into the culture medium or by diesel exhaust administered at an air-liquid interface. When A549 cells were exposed to DPM in the culture medium, cell proliferation was inhibited at doses of 10-100 μg/mL; generation of interleukin (IL)-8 and the antioxidant enzyme, heme oxygenase-1 (HO-1), were inhibited at a dose of 100 μg/mL, and hydroxyl radicals were produced, but could be inhibited by catalase or superoxide dismutase. In contrast, when A549 cells were exposed to diesel exhaust, cell proliferation was inhibited in the absence, but not in the presence, of a diesel particulate filter (DPF); in the absence of a DPF IL-8 was produced in the same amount as in the control cells but was suppressed in the presence of a DPF; HO-1 mRNA was transiently over-expressed in the presence of a DPF, and it was also increased slightly produced in the absence of a DPF but statistically not significant in the presence of a DPF, and it was also increased slightly produced in the absence of a DPF but statistically not significant; HO-1 was transiently produced independent of the absence or the presence of a DPF; and hydroxyl radicals were weakly produced, even in the presence of a DPF but could be inhibited by catalase or superoxide dismutase. It is thus suggested that oxidative stress may be induced by exposure to DPM or diesel exhaust and thereby exerts cytotoxic effect. The introduction of a DPF is effective to protect cells from the toxicity of diesel exhaust presumably by suppression of an oxidative stress.
authors.affiliation: Division of Environmental Health, Tokyo Metropolitan Institute of Public Health, 3-24-1 Hyakunin'cho, Shin'juku-ku, Tokyo 169-0073, Japan. Electronic address: Tomoko_Ookubo@member.metro.tokyo.jp.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Okubo
authors.affiliation: Division of Environmental Health, Tokyo Metropolitan Institute of Public Health, 3-24-1 Hyakunin'cho, Shin'juku-ku, Tokyo 169-0073, Japan.
authors.forename: Mitsugu
authors.initials: M
authors.lastname: Hosaka
authors.affiliation: Department of Nutritional Science and Food Safety, Faculty of Applied Biosciences, Tokyo University of Agriculture, 1-1-1 Sakura-ga-Oka, Setagtaya, Tokyo 156-8502, Japan. Electronic address: agalennde.dai@nifty.com.
authors.forename: Dai
authors.initials: D
authors.lastname: Nakae
mesh_terms: D002282:Adenocarcinoma, Bronchiolo-Alveolar / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology; D001335:Vehicle Emissions / Q000633:toxicity*
chemical_list: D001335:Vehicle Emissions
keywords: A549 cell; Diesel exhaust; Diesel particulate filter; Heme oxygenase-1; Interleukin-8