title: Two proteins of Cucurbit chlorotic yellows virus, P59 and P9, are self-interacting.
abstract: The yeast two-hybrid (Y2H) assay, a powerful tool for identifying protein-protein interactions, has been widely used to study viral protein interactions and to elucidate the functions of viral proteins. In this study, Cucurbit chlorotic yellows virus-encoded proteins were investigated by Y2H assays in all possible pairwise combinations, and the self-interactions of P59 and P9 were detected. The interacting domains of P59 and P9 were identified using vectors carrying an activation domain fused to a truncated version of P59 or P9. We found that the middle region (amino acids 173-344) of P59 was necessary for this self-interaction, while three different truncated versions of P9 showed no interaction with full-length P9. This is the first report of the self-interaction of P59 in the genus Crinivirus.
authors.affiliation: College of Plant Protection, Henan Agricultural University, Zhengzhou, 450002, China.
authors.forename: Zhenyue
authors.initials: Z
authors.lastname: Wang
authors.forename: Yingzhi
authors.initials: Y
authors.lastname: Wang
authors.forename: Hu
authors.initials: H
authors.lastname: Sun
authors.forename: Qinsheng
authors.initials: Q
authors.lastname: Gu
authors.forename: Honglian
authors.initials: H
authors.lastname: Li
authors.forename: Bingjian
authors.initials: B
authors.lastname: Sun
authors.forename: Yajuan
authors.initials: Y
authors.lastname: Shi
authors.forename: Yan
authors.initials: Y
authors.lastname: Shi
mesh_terms: D029383:Crinivirus / Q000502:physiology*; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D025941:Protein Interaction Mapping; D020798:Two-Hybrid System Techniques; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D014764:Viral Proteins