title: Supramolecular Control in Nanostructured Film Architectures for Detecting Breast Cancer.
abstract: The need for early detection of various diseases, including breast cancer, has motivated research into nanomaterials that can be assembled in organized films which serve as biosensors. Owing to the variety of possible materials and film architectures, procedures are required to design optimized biosensors. In this study, we combine surface-specific methods to monitor the assembly of antibodies on nanostructured films with two distinct architectures. In the first, a layer of the antibody type mouse anti-HER2 (clone tab250) was immobilized on a self-assembled monolayer (SAM) of 11-mercaptoundecanoic acid modified with N-hydroxysuccinimide (NHS) and 1-ethyl-3-(3-(dimethylamino)propyl)carbodiimide (EDC). In the second approach, a SAM of cysteamine was coated with a biotin/spreptavidin bilayer on which a layer of biotinylated antibody type MSx2HUp185/her biotin was adsorbed. The rougher, less passivating coating with cysteamine determined from cyclic voltammetry and scanning electron microscopy led to biosensors that are more sensitive to detect the breast cancer ERBB2 (HER2) biomarker in impedance spectroscopy measurements. This higher distinguishing ability of the cysteamine-containing film architecture was proven with information visualization methods to treat the impedance data. Polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) confirmed that biosensing resulted from the antibody-ERBB2 antigen affinity.
authors.affiliation: †São Carlos Institute of Physics, University of São Paulo, 369, 13560-970 São Carlos, São Paulo, Brazil.
authors.forename: Juliana Coatrini
authors.initials: JC
authors.lastname: Soares
authors.affiliation: †São Carlos Institute of Physics, University of São Paulo, 369, 13560-970 São Carlos, São Paulo, Brazil.
authors.forename: Flavio Makoto
authors.initials: FM
authors.lastname: Shimizu
authors.affiliation: †São Carlos Institute of Physics, University of São Paulo, 369, 13560-970 São Carlos, São Paulo, Brazil.
authors.forename: Andrey Coatrini
authors.initials: AC
authors.lastname: Soares
authors.affiliation: ‡Institute of Environmental, Chemical and Pharmaceutical Sciences, Federal University of Sao Paulo, 09972-970 Diadema, São Paulo, Brazil.
authors.forename: Luciano
authors.initials: L
authors.lastname: Caseli
authors.affiliation: §Institute of Chemistry, Federal University of Rio Grande do Sul, 15003, 91501-970 Porto Alegre, Rio Grande do Sul, Brazil.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Ferreira
authors.affiliation: †São Carlos Institute of Physics, University of São Paulo, 369, 13560-970 São Carlos, São Paulo, Brazil.
authors.forename: Osvaldo N
authors.initials: ON
authors.lastname: Oliveira
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry* / Q000276:immunology / Q000494:pharmacology; D015374:Biosensing Techniques; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000473:pathology; D002234:Carbodiimides / Q000737:chemistry; D058266:Dielectric Spectroscopy; D004123:Dimethylamines / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D005260:Female; D006046:Gold / Q000737:chemistry; D006801:Humans; D051379:Mice; D049329:Nanostructures / Q000737:chemistry* / Q000627:therapeutic use; D013388:Succinimides / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: C505222:11-mercaptoundecanoic acid; D000906:Antibodies; D002234:Carbodiimides; D004123:Dimethylamines; D005227:Fatty Acids; C569266:N-ethyl-N'-(3-(dimethylamino)-propyl)carbodiimide; D013388:Succinimides; D013438:Sulfhydryl Compounds; D006046:Gold; C001426:N-hydroxysuccinimide
keywords: biosensors; breast cancer; impedance spectroscopy; information visualization; nanostructured films