title: Automated acquisition and analysis of airway surface liquid height by confocal microscopy.
abstract: The airway surface liquid (ASL) is a thin-liquid layer that lines the luminal side of airway epithelia. ASL contains many molecules that are involved in primary innate defense in the lung. Measurement of ASL height on primary airway cultures by confocal microscopy is a powerful tool that has enabled researchers to study ASL physiology and pharmacology. Previously, ASL image acquisition and analysis were performed manually. However, this process is time and labor intensive. To increase the throughput, we have developed an automatic ASL measurement technique that combines a fully automated confocal microscope with novel automatic image analysis software that was written with image processing techniques derived from the computer science field. We were able to acquire XZ ASL images at the rate of ∼ 1 image/s in a reproducible fashion. Our automatic analysis software was able to analyze images at the rate of ∼ 32 ms/image. As proofs of concept, we generated a time course for ASL absorption and a dose response in the presence of SPLUNC1, a known epithelial sodium channel inhibitor, on human bronchial epithelial cultures. Using this approach, we determined the IC50 for SPLUNC1 to be 6.53 μM. Furthermore, our technique successfully detected a difference in ASL height between normal and cystic fibrosis (CF) human bronchial epithelial cultures and detected changes in ATP-stimulated Cl(-)/ASL secretion. We conclude that our automatic ASL measurement technique can be applied for repeated ASL height measurements with high accuracy and consistency and increased throughput.
authors.affiliation: Department of Electronic Engineering, Yeungnam University, Kyungsan, Kyungbuk, South Korea; and.
authors.forename: Hyun-Chul
authors.initials: HC
authors.lastname: Choi
authors.affiliation: Cystic Fibrosis Center/Marsico Lung Institute, University of North Carolina, Chapel Hill, North Carolina.
authors.forename: Christine Seul Ki
authors.initials: CS
authors.lastname: Kim
authors.affiliation: Cystic Fibrosis Center/Marsico Lung Institute, University of North Carolina, Chapel Hill, North Carolina tarran@med.unc.edu.
authors.forename: Robert
authors.initials: R
authors.lastname: Tarran
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D001980:Bronchi / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D003550:Cystic Fibrosis / Q000378:metabolism*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D017136:Ion Transport; D018613:Microscopy, Confocal; D010750:Phosphoproteins / Q000378:metabolism*; D020545:Respiratory Mucosa / Q000378:metabolism* / Q000648:ultrastructure; D012964:Sodium / Q000378:metabolism
chemical_list: C485599:BPIFA1 protein, human; D006023:Glycoproteins; D010750:Phosphoproteins; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D000255:Adenosine Triphosphate; D012964:Sodium
keywords: CFTR; COPD; ENaC; airway surface liquid; cystic fibrosis; fluorescent microscopy