title: ISAC's Gating-ML 2.0 data exchange standard for gating description.
abstract: The lack of software interoperability with respect to gating has traditionally been a bottleneck preventing the use of multiple analytical tools and reproducibility of flow cytometry data analysis by independent parties. To address this issue, ISAC developed Gating-ML, a computer file format to encode and interchange gates. Gating-ML 1.5 was adopted and published as an ISAC Candidate Recommendation in 2008. Feedback during the probationary period from implementors, including major commercial software companies, instrument vendors, and the wider community, has led to a streamlined Gating-ML 2.0. Gating-ML has been significantly simplified and therefore easier to support by software tools. To aid developers, free, open source reference implementations, compliance tests, and detailed examples are provided to stimulate further commercial adoption. ISAC has approved Gating-ML as a standard ready for deployment in the public domain and encourages its support within the community as it is at a mature stage of development having undergone extensive review and testing, under both theoretical and practical conditions.
authors.affiliation: Terry Fox Laboratory, BC Cancer Agency, Vancouver, British Columbia, Canada.
authors.forename: Josef
authors.initials: J
authors.lastname: Spidlen
authors.affiliation: Genetics Department, Stanford University School of Medicine, Stanford, California.
authors.forename: Wayne
authors.initials: W
authors.lastname: Moore
authors.affiliation: Terry Fox Laboratory, BC Cancer Agency, Vancouver, British Columbia, Canada.|Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia, Canada.
authors.forename: Ryan R
authors.initials: RR
authors.lastname: Brinkman
mesh_terms: D019295:Computational Biology / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods* / Q000592:standards*; D012015:Reference Standards; D015203:Reproducibility of Results; D012984:Software / Q000592:standards*
keywords: bioinformatics; data standard; file format; flow cytometry; gating