title: [Effect of total saponins from rhizoma dioscoreae nipponicae on the expression of SDF1 and HCIKB kinase in rIK-1beta induced fibroblast-like synoviocytes].
abstract: OBJECTIVE
  To study the effect of total saponins from Rhizoma Dioscoreae nipponicae (RDN) on the expression of stroma cell derived factor 1 (SDF1) and IKB kinase (IKK) in rIL-1beta induced fibroblast-like synoviocytes (FLS).
  
  
  METHODS
  FLS were primarily cultured and the 3rd generation log phase growth FLS were divided into the normal control group, the model group, and the medication group. 10 microg/L rIL-1beta was used to induce the proliferation of FLS in the model group.10 microg/L rIL-1beta and 100 microg/L RDN were administered to co-incubate FLS in the medication group. No treatment was given to FLS in the normal control group. Expression levels of SDF1 and IkapaB kinase proteins (p-IKK) were detected using Western blot.
  
  
  RESULTS
  Expression levels of SDF1 and p-IKK increased significantly higher in the model group than in the normal control group (P<0.01). Compared with the model group, expression levels of SDF-1 and p-IKK significantly decreased in the medication group (P <0.01).
  
  
  CONCLUSIONS
  Total saponins from RDN could inhibit the activation of both SDF1 and p-IKK. It might further regulate the expression of IKB kinase by regulating the expression of SDF1.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhou
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.forename: Fang
authors.initials: F
authors.lastname: Lu
authors.forename: Shu-min
authors.initials: SM
authors.lastname: Liu
mesh_terms: D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000378:metabolism; D027742:Dioscorea; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D004847:Epithelial Cells; D005347:Fibroblasts; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism; D012503:Saponins / Q000378:metabolism; D013583:Synovial Membrane / Q000378:metabolism*
chemical_list: D054377:Chemokine CXCL12; D004365:Drugs, Chinese Herbal; D053583:Interleukin-1beta; D012503:Saponins