title: Identification of human basic fetoprotein as glucose-6-phosphate isomerase by using N- and C-terminal sequence tags and terminal tag database.
abstract: Human basic fetoprotein (BFP), found in fetal serum and tissue extracts as well as in extracts of various cancer tissues, has long been known as a marker protein for cancers; however, the primary sequence has not yet been reported. This paper describes the identification of BFP using the N- and C-terminal amino acid sequence tags (Ac-AALTRDPQFQ and QQREARVQ, respectively) clarified by mass spectrometry-based methods, and a terminal tag database (ProteinCarta). In this study, BFP was identified as glucose-6-phosphate isomerase (G6PI_HUMAN).
authors.affiliation: Koichi Tanaka Laboratory of Advanced Science and Technology, Shimadzu Corporation, 1, Nishinokyo-Kuwabaracho, Nakagyo-ku, Kyoto 604-8511, Japan. Electronic address: kuyama@shimadzu.co.jp.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kuyama
authors.affiliation: Koichi Tanaka Laboratory of Advanced Science and Technology, Shimadzu Corporation, 1, Nishinokyo-Kuwabaracho, Nakagyo-ku, Kyoto 604-8511, Japan. Electronic address: acyshzw@shimadzu.co.jp.
authors.forename: Akiyasu C
authors.initials: AC
authors.lastname: Yoshizawa
authors.affiliation: Koichi Tanaka Laboratory of Advanced Science and Technology, Shimadzu Corporation, 1, Nishinokyo-Kuwabaracho, Nakagyo-ku, Kyoto 604-8511, Japan.
authors.forename: Chihiro
authors.initials: C
authors.lastname: Nakajima
authors.affiliation: Koichi Tanaka Laboratory of Advanced Science and Technology, Shimadzu Corporation, 1, Nishinokyo-Kuwabaracho, Nakagyo-ku, Kyoto 604-8511, Japan.
authors.forename: Mutsumi
authors.initials: M
authors.lastname: Hosako
authors.affiliation: Koichi Tanaka Laboratory of Advanced Science and Technology, Shimadzu Corporation, 1, Nishinokyo-Kuwabaracho, Nakagyo-ku, Kyoto 604-8511, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Tanaka
mesh_terms: D000595:Amino Acid Sequence; D030562:Databases, Protein; D005326:Fetal Proteins / Q000737:chemistry*; D019298:Glucose-6-Phosphate / Q000737:chemistry*; D006801:Humans; D007535:Isomerases / Q000737:chemistry*; D008969:Molecular Sequence Data; D020539:Sequence Analysis, Protein / Q000379:methods
chemical_list: D005326:Fetal Proteins; D019298:Glucose-6-Phosphate; D007535:Isomerases
keywords: Basic fetoprotein; MALDI mass spectrometry; N- and C-terminal sequencing; Terminal tag database; protein identification