title: Occurrence and new mutations involved in rifampicin-resistant Propionibacterium acnes strains isolated from biofilm or device-related infections.
abstract: We described for the first time the amino acid substitutions conferring rifampicin resistance in eight Propionibacterium acnes strains isolated from patients with biofilm or device-related infections. We identified different mutations in cluster I and one mutation, never reported, in cluster II of the rpoB gene (I480V) associated with the most frequent one in cluster I (S442L). Half of the patients previously received treatment with rifampicin.
authors.affiliation: Septic Surgical Unit, Department of Surgery and Anaesthesiology, Lausanne University Hospital, Lausanne, Switzerland.
authors.forename: Ulrika
authors.initials: U
authors.lastname: Furustrand Tafin
authors.affiliation: Service de Bactériologie-Hygiène Hospitalière, Nantes University Hospital, Nantes, France; Université de Nantes, EA3826 Thérapeutiques cliniques et expérimentales des infections, Nantes Medicine School, F-44000 Nantes, France.
authors.forename: Guillaume Ghislain
authors.initials: GG
authors.lastname: Aubin
authors.affiliation: Infectious Diseases Service, Zurich University Hospital, Zurich, Switzerland.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Eich
authors.affiliation: Center for Musculoskeletal Surgery, Charité - University Medicine Berlin, Berlin, Germany.
authors.forename: Andrej
authors.initials: A
authors.lastname: Trampuz
authors.affiliation: Service de Bactériologie-Hygiène Hospitalière, Nantes University Hospital, Nantes, France; Université de Nantes, EA3826 Thérapeutiques cliniques et expérimentales des infections, Nantes Medicine School, F-44000 Nantes, France. Electronic address: stephane.corvec@chu-nantes.fr.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Corvec
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019943:Amino Acid Substitution; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D018441:Biofilms; D055499:Catheter-Related Infections / Q000382:microbiology*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D024881:Drug Resistance, Bacterial; D005260:Female; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D011425:Propionibacterium acnes / Q000187:drug effects* / Q000302:isolation & purification; D012293:Rifampin / Q000494:pharmacology*; D017422:Sequence Analysis, DNA
chemical_list: D000900:Anti-Bacterial Agents; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit; D012293:Rifampin
keywords: Biofilm; Foreign-body infection; Propionibacterium acnes; Resistance; Rifampicin