title: Fast size-determination of intact bacterial plasmids using nanofluidic channels.
abstract: We demonstrate how nanofluidic channels can be used as a tool to rapidly determine the number and sizes of plasmids in bacterial isolates. Each step can be automated at low cost, opening up opportunities for general use in microbiology labs.
authors.affiliation: Department of Biology and Biological Engineering, Chalmers University of Technology, SE-412 96, Gothenburg, Sweden. fredrik.westerlund@chalmers.se.
authors.forename: K
authors.initials: K
authors.lastname: Frykholm
authors.forename: L K
authors.initials: LK
authors.lastname: Nyberg
authors.forename: E
authors.initials: E
authors.lastname: Lagerstedt
authors.forename: C
authors.initials: C
authors.lastname: Noble
authors.forename: J
authors.initials: J
authors.lastname: Fritzsche
authors.forename: N
authors.initials: N
authors.lastname: Karami
authors.forename: T
authors.initials: T
authors.lastname: Ambjörnsson
authors.forename: L
authors.initials: L
authors.lastname: Sandegren
authors.forename: F
authors.initials: F
authors.lastname: Westerlund
mesh_terms: D001419:Bacteria / Q000235:genetics*; D001583:Benzoxazoles / Q000737:chemistry; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry; D036103:Nanotechnology / Q000295:instrumentation; D010957:Plasmids / Q000378:metabolism*; D011806:Quinolinium Compounds / Q000737:chemistry
chemical_list: D001583:Benzoxazoles; D004269:DNA, Bacterial; D011806:Quinolinium Compounds; C075296:1,1'-((4,4,7,7-tetramethyl)-4,7-diazaundecamethylene)bis-4-(3-methyl-2,3-dihydro(benzo-1,3-oxazole)-2-methylidene)quinolinium