title: Virus testing by PCR and RT-PCR amplification in berry fruit.
abstract: Berry fruit crops are prone to infection by a wide range of viruses, with the list expanding every year, primarily because of the expansion of the crops to new geographic regions. Although some methods allow for virus detection in a nonspecific manner, the advent of cheap and effective nucleic acid sequencing technologies has allowed for the development of species-specific tests. This chapter describes methods for extraction of nucleic acids for molecular testing from a range of different berry fruit crops and lists oligonucleotide primers that have been developed for amplification of a large number of berry fruit viruses.
authors.affiliation: Cell and Molecular Sciences Group, The James Hutton Institute, Invergowrie, Dundee, DD2 5DA, UK, s.macfarlane@hutton.ac.uk.
authors.forename: Stuart
authors.initials: S
authors.lastname: MacFarlane
authors.forename: Wendy
authors.initials: W
authors.lastname: McGavin
authors.forename: Ioannis
authors.initials: I
authors.lastname: Tzanetakis
mesh_terms: D005638:Fruit / Q000235:genetics / Q000382:microbiology* / Q000821:virology*; D010935:Plant Diseases / Q000821:virology; D010942:Plant Viruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000032:analysis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity
chemical_list: D012367:RNA, Viral