title: Effects of dietary selenium on apoptosis of germ cells in the testis during spermatogenesis in roosters.
abstract: The aim of this study was to determine the effects of dietary selenium (Se) supplementation on apoptosis of germ cells in the testis during spermatogenesis in roosters. Eighty 12-week-old Hy-Line Variety white roosters with an averaged body weight of 1.38 ± 0.2 kg were selected and randomly divided into four experimental groups. They were fed the basal diet (0.044 mg/kg Se dry matter) supplemented with 0 (control), 0.5, 1.0, or 2.0 mg/kg of Se dry matter (from sodium selenite). After the 45-day feeding experiment, testis samples were collected from the roosters of each treatment group to detect the population of apoptotic germ cells using the terminal deoxynucleotidy1 transferase dUTP nick end labeling assay. The protein expression of cell cycle-related genes and the messenger RNA (mRNA) expression of apoptosis and cell cycle-related genes had also been detected. The results show that the population of apoptotic germ cells in the control and 2.0 mg/kg groups was increased (P < 0.05) compared with that in the 0.5 mg/kg and 1.0 mg/kg groups. Expressions of CDC2 and CCNB1 protein in the control and 2.0 mg/kg groups were lower (P < 0.05) than those in the 0.5 mg/kg and 1.0 mg/kg groups. The mRNA level of CDC2 in the 0.5 mg/kg group was higher (P < 0.05) than that in other groups. The lowest (P < 0.05) mRNA expressions of apoptosis-related genes (BCL-2, CASPASE 3, CASPASE 8) were also obtained in the 0.5 mg/kg group. These results show that dietary Se of roosters can affect apoptosis of germ cells by regulating the mRNA expressions of apoptosis- and cell cycle-related genes in the testis during spermatogenesis.
authors.affiliation: College of Animal Science and Technology, Shanxi Agricultural University, Taigu, PR China.
authors.forename: Ruigao
authors.initials: R
authors.lastname: Song
authors.affiliation: College of Animal Science and Technology, Shanxi Agricultural University, Taigu, PR China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Yao
authors.affiliation: College of Animal Science and Technology, Shanxi Agricultural University, Taigu, PR China; Lab of Animal Reproduction, Shanxi Agricultural University, Taigu, PR China. Electronic address: regan711@hotmail.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
authors.affiliation: College of Animal Science and Technology, Shanxi Agricultural University, Taigu, PR China; Lab of Animal Reproduction, Shanxi Agricultural University, Taigu, PR China.
authors.forename: Youshe
authors.initials: Y
authors.lastname: Ren
authors.affiliation: College of Animal Science and Technology, Shanxi Agricultural University, Taigu, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
mesh_terms: D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D002645:Chickens / Q000502:physiology*; D004032:Diet / Q000662:veterinary; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000502:physiology; D005854:Germ Cells / Q000187:drug effects*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012643:Selenium / Q000008:administration & dosage / Q000494:pharmacology*; D013091:Spermatogenesis / Q000502:physiology*; D013737:Testis / Q000502:physiology*
chemical_list: D018797:Cell Cycle Proteins; D012333:RNA, Messenger; D012643:Selenium
keywords: Apoptosis; Germ cell; Rooster; Selenium; Spermatogenesis