title: Immune modulation of glycosaminoglycan derived from P. lewisi in TNF-α stimulated cells.
abstract: Poecilocoris lewisi (Korean name: "Kwangdaenolinjae") is a red-striped gold stink bug (insect) which has been used as a crude drug in traditional medicine of East Asia and Korea. In this study, ethanol extract and glycosaminoglycan from P. lewisi (Pl GAG), as an active substance among its components, were investigated for their potential anti-inflammatory actions. They were found to be a potent inducer of nitric oxide (NO) production from calf pulmonary artery endothelial (CPAE) cells and a stimulator of endothelial nitric oxide synthase in a dose-dependent manner. The anti-inflammatory activities were also evaluated by determining the level of adhesion molecules related to atherogenesis and pro-inflammatory cytokines, such as vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1), secretory phospholipase A2, and prostaglandin E2, stimulated by tumor necrosis factor (TNF)-α in human umbilical vein endothelial cells (HUVEC). They also showed inhibitory effects on vascular endothelial growth factor (VEGF) production in HUVECs. Matrix metalloproteinases (MMP-2 and 9) were also inhibited by treatment with this extract or glycosaminoglycan. Furthermore, this GAG showed cytotoxicity against CT-26 colon cancer cells whereas having no cytotoxicity in CHO normal cells. Monosaccharide (amino, acidic, neutral monosaccharides) composition of used GAG was characterized by trimethylsilylated GC-MS analysis method.
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, RDA, 166 Nongsaengmyung-Ro, Iseo-Myun, Wanju-gun, 585-851, South Korea. amy@korea.kr.
authors.forename: Mi-Young
authors.initials: MY
authors.lastname: Ahn
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, RDA, 166 Nongsaengmyung-Ro, Iseo-Myun, Wanju-gun, 585-851, South Korea.
authors.forename: Soon-Ja
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, RDA, 166 Nongsaengmyung-Ro, Iseo-Myun, Wanju-gun, 585-851, South Korea.
authors.forename: NamJung
authors.initials: N
authors.lastname: Kim
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, RDA, 166 Nongsaengmyung-Ro, Iseo-Myun, Wanju-gun, 585-851, South Korea.
authors.forename: Jae Sam
authors.initials: JS
authors.lastname: Hwang
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, RDA, 166 Nongsaengmyung-Ro, Iseo-Myun, Wanju-gun, 585-851, South Korea.
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Yun
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D016466:CHO Cells; D002417:Cattle; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000188:drug therapy / Q000473:pathology; D006224:Cricetinae; D003412:Cricetulus; D016207:Cytokines / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000187:drug effects* / Q000276:immunology; D008401:Gas Chromatography-Mass Spectrometry; D006025:Glycosaminoglycans / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D020062:Heteroptera / Q000378:metabolism*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D009569:Nitric Oxide / Q000378:metabolism; D011651:Pulmonary Artery / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000008:administration & dosage; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D006025:Glycosaminoglycans; D014409:Tumor Necrosis Factor-alpha; D042461:Vascular Endothelial Growth Factor A; D009569:Nitric Oxide
keywords: Ethanol extract; Glycosaminoglycan; Immune modulation; P. lewisi