title: Keratitis caused by moulds in Santa Lucía Ophthalmology Hospital in Buenos Aires, Argentina.
abstract: BACKGROUND
  Mycotic keratitis by moulds (MKM) is an important cause for corneal blindness and usually carries an unfavorable prognosis.
  
  
  OBJECTIVE
  This study describes the risk factors and demographic and microbiological features of all MKM cases in Santa Lucía Ophthalmology Hospital during a period of 6 years.
  
  
  METHODS
  A prospective study was performed for all MKM cases diagnosed between October 2007 and September 2013.
  
  
  RESULTS
  Among 157 diagnosed cases, direct microscopic examination and culture were positive in 97 and 96% of the cases respectively. MKM represents 17% of all microbiologically confirmed corneal abscesses. No significant differences were detected in annual MKM frequencies across the study period, suggesting that MKM incidence remains constant over time. A male-to-female ratio was observed (2.8:1); the most affected age groups ranged from 31 to 40 years old (males) and 61-70 years old (females). The most frequent predisposing factor was trauma (40%) followed by the use of contact lenses (9%), herpetic abscesses (5%) and diabetes (4%). The predominant genera were Fusarium (66%), Aspergillus (10%), Curvularia (6%) and Alternaria (4%). The most frequent agent was Fusarium solani species complex (52%). More than two-thirds of the cases were produced by only 3 species or complexes. However, at least 29 different species were detected in the remaining cases. This is the first report of Pholiota sp. as causative agent of human MKM.
  
  
  CONCLUSIONS
  Argentina lacks extensive epidemiological and clinical data on MKM. This six-year study performed in Argentina is a first step leading to a better understanding of MKM epidemiology in our country.
authors.affiliation: Departamento de Micología, Instituto Nacional de Enfermedades Infecciosas (INEI) Dr. Carlos G. Malbrán, Administración Nacional de Laboratorios e Institutos de Salud (ANLIS), Ciudad Autónoma de Buenos Aires, Argentina. Electronic address: nrefojo@anlis.gov.ar.
authors.forename: Nicolás
authors.initials: N
authors.lastname: Refojo
authors.affiliation: Hospital Oftalmológico Santa Lucía, Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Patricia
authors.initials: P
authors.lastname: Minervini
authors.affiliation: Departamento de Micología, Instituto Nacional de Enfermedades Infecciosas (INEI) Dr. Carlos G. Malbrán, Administración Nacional de Laboratorios e Institutos de Salud (ANLIS), Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Alejandra I
authors.initials: AI
authors.lastname: Hevia
authors.affiliation: Departamento de Micología, Instituto Nacional de Enfermedades Infecciosas (INEI) Dr. Carlos G. Malbrán, Administración Nacional de Laboratorios e Institutos de Salud (ANLIS), Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Ruben A
authors.initials: RA
authors.lastname: Abrantes
authors.affiliation: Departamento de Micología, Instituto Nacional de Enfermedades Infecciosas (INEI) Dr. Carlos G. Malbrán, Administración Nacional de Laboratorios e Institutos de Salud (ANLIS), Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Julián
authors.initials: J
authors.lastname: Fernández
authors.affiliation: Hospital Oftalmológico Santa Lucía, Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Norma
authors.initials: N
authors.lastname: Apestey
authors.affiliation: Hospital Oftalmológico Santa Lucía, Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Mariana
authors.initials: M
authors.lastname: Garnero
authors.affiliation: Hospital Oftalmológico Santa Lucía, Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Margarita
authors.initials: M
authors.lastname: Villada
authors.affiliation: Departamento de Micología, Instituto Nacional de Enfermedades Infecciosas (INEI) Dr. Carlos G. Malbrán, Administración Nacional de Laboratorios e Institutos de Salud (ANLIS), Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Graciela
authors.initials: G
authors.lastname: Davel
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001118:Argentina; D002648:Child; D015821:Eye Infections, Fungal / Q000453:epidemiology; D005260:Female; D006782:Hospitals, Special; D006801:Humans; D007634:Keratitis / Q000453:epidemiology / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D009885:Ophthalmology; D011446:Prospective Studies; D012307:Risk Factors; D013997:Time Factors; D055815:Young Adult
keywords: Factores predisponentes; Fungal keratitis; Fusarium; Predisposing factors; Queratitis fúngica