title: Implantable vaccine development using in vitro antigen-pulsed macrophages absorbed on laser micro-structured Si scaffolds.
abstract: To overcome the limiting antigenic repertoire of protein sub-units and the side effects of adjuvants applied in second generation vaccines, the present work combined in vitro and in vivo manipulations to develop biomaterials allowing natural antigen-loading and presentation in vitro and further activation of the immune response in vivo. 3-dimensional laser micro-textured implantable Si-scaffolds supported mouse macrophage adherence, allowed natural seeding with human serum albumin (antigen) and specific antibody and inflammatory cytokine production in vitro. Implantation of Si-scaffolds loaded with antigen-activated macrophages induced an inflammatory reaction along with antigen-specific antibody production in vivo, which could be detected even 30 days post implantation. Analysis of implant histology using scanning electron microscopy showed that Si-scaffolds could be stable for a 6-month period. Such technology leads to personalized implantable vaccines, opening novel areas of research and treatment.
authors.affiliation: Department of Biology, University of Crete, Vassilika Vouton, Heraklion 71409, Crete, Greece.
authors.forename: Ioanna
authors.initials: I
authors.lastname: Zerva
authors.affiliation: Department of Biology, University of Crete, Vassilika Vouton, Heraklion 71409, Crete, Greece; Institute of Electronic Structure and Laser (IESL), Foundation for Research and Technology, Vassilika Vouton, Heraklion 71409, Crete, Greece.
authors.forename: Chara
authors.initials: C
authors.lastname: Simitzi
authors.affiliation: Department of Biology, University of Crete, Vassilika Vouton, Heraklion 71409, Crete, Greece.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Siakouli-Galanopoulou
authors.affiliation: Institute of Electronic Structure and Laser (IESL), Foundation for Research and Technology, Vassilika Vouton, Heraklion 71409, Crete, Greece.
authors.forename: Anthi
authors.initials: A
authors.lastname: Ranella
authors.affiliation: Institute of Electronic Structure and Laser (IESL), Foundation for Research and Technology, Vassilika Vouton, Heraklion 71409, Crete, Greece.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Stratakis
authors.affiliation: Institute of Electronic Structure and Laser (IESL), Foundation for Research and Technology, Vassilika Vouton, Heraklion 71409, Crete, Greece; Department of Physics, University of Crete, Vassilika Vouton, Heraklion 71409, Crete, Greece.
authors.forename: Costas
authors.initials: C
authors.lastname: Fotakis
authors.affiliation: Department of Biology, University of Crete, Vassilika Vouton, Heraklion 71409, Crete, Greece. Electronic address: athan@biology.uoc.gr.
authors.forename: Irene
authors.initials: I
authors.lastname: Athanassakis
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology / Q000378:metabolism; D002448:Cell Adhesion; D017690:Cell Transplantation; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology* / Q000502:physiology*; D008297:Male; D008807:Mice, Inbred BALB C; D054457:Tissue Scaffolds; D016896:Treatment Outcome; D014611:Vaccination / Q000379:methods*; D014612:Vaccines / Q000008:administration & dosage*
chemical_list: D000941:Antigens; D014612:Vaccines
keywords: Immune response; Laser manufacturing; Macrophage; Scaffold implantation; Silicon scaffold; Vaccination