title: Immunostimulatory Effects Triggered by Enterococcus faecalis CECT7121 Probiotic Strain Involve Activation of Dendritic Cells and Interferon-Gamma Production.
abstract: Probiotics can modulate the immune system, conferring beneficial effects on the host. Understanding how these microorganisms contribute to improve the health status is still a challenge. Previously, we have demonstrated that Enterococcus faecalis CECT7121 implants itself and persists in the murine gastrointestinal tract, and enhances and skews the profile of cytokines towards the Th1 phenotype in several biological models. Given the importance of dendritic cells (DCs) in the orchestration of immunity, the aim of this work was to elucidate the influence of E. faecalis CECT7121 on DCs and the outcome of the immune responses. In this work we show that E. faecalis CECT7121 induces a strong dose-dependent activation of DCs and secretion of high levels of IL-12, IL-6, TNFα, and IL-10. This stimulation is dependent on TLR signaling, and skews the activation of T cells towards the production of IFNγ. The influence of this activation in the establishment of Th responses in vivo shows the accumulation of specific IFNγ-producing cells. Our findings indicate that the activation exerted by E. faecalis CECT7121 on DCs and its consequence on the cellular adaptive immune response may have broad therapeutic implications in immunomodulation.
authors.affiliation: Laboratorio de Modulación de la Respuesta Inmune, IDEHU (CONICET-UBA). Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Matías Alejandro
authors.initials: MA
authors.lastname: Molina
authors.affiliation: Laboratorio de Modulación de la Respuesta Inmune, IDEHU (CONICET-UBA). Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Ailén Magalí
authors.initials: AM
authors.lastname: Díaz
authors.affiliation: Institute of Infection Immunology, TWINCORE, Centre for Experimental and Clinical Infection Research, a Joint Venture between the Medical School Hanover (MHH) and the Helmholtz Centre for Infection Research (HZI), Hannover, Germany.
authors.forename: Christina
authors.initials: C
authors.lastname: Hesse
authors.affiliation: Institute of Infection Immunology, TWINCORE, Centre for Experimental and Clinical Infection Research, a Joint Venture between the Medical School Hanover (MHH) and the Helmholtz Centre for Infection Research (HZI), Hannover, Germany.
authors.forename: Wiebke
authors.initials: W
authors.lastname: Ginter
authors.affiliation: Instituto de Inmunología, Genética y Metabolismo, INIGEM (CONICET-UBA). Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: María Virginia
authors.initials: MV
authors.lastname: Gentilini
authors.affiliation: Laboratorio de Modulación de la Respuesta Inmune, IDEHU (CONICET-UBA). Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Guillermo Gabriel
authors.initials: GG
authors.lastname: Nuñez
authors.affiliation: Laboratorio de Anticuerpos Asimétricos e Inmunología de la Reproducción, IDEHU (CONICET-UBA). Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Andrea Mercedes
authors.initials: AM
authors.lastname: Canellada
authors.affiliation: Institute of Infection Immunology, TWINCORE, Centre for Experimental and Clinical Infection Research, a Joint Venture between the Medical School Hanover (MHH) and the Helmholtz Centre for Infection Research (HZI), Hannover, Germany.
authors.forename: Tim
authors.initials: T
authors.lastname: Sparwasser
authors.affiliation: Institute of Infection Immunology, TWINCORE, Centre for Experimental and Clinical Infection Research, a Joint Venture between the Medical School Hanover (MHH) and the Helmholtz Centre for Infection Research (HZI), Hannover, Germany.
authors.forename: Luciana
authors.initials: L
authors.lastname: Berod
authors.affiliation: Laboratorio de Modulación de la Respuesta Inmune, IDEHU (CONICET-UBA). Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Marisa Silvia
authors.initials: MS
authors.lastname: Castro
authors.affiliation: Laboratorio de Modulación de la Respuesta Inmune, IDEHU (CONICET-UBA). Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Marcela Alejandra
authors.initials: MA
authors.lastname: Manghi
mesh_terms: D000818:Animals; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology*; D013293:Enterococcus faecalis / Q000276:immunology*; D041981:Gastrointestinal Tract / Q000187:drug effects / Q000276:immunology / Q000382:microbiology; D007109:Immunity / Q000187:drug effects / Q000276:immunology; D056747:Immunomodulation / Q000187:drug effects* / Q000276:immunology*; D007371:Interferon-gamma / Q000276:immunology*; D016753:Interleukin-10 / Q000276:immunology; D018664:Interleukin-12 / Q000276:immunology; D015850:Interleukin-6 / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D019936:Probiotics / Q000008:administration & dosage*; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D018664:Interleukin-12; D007371:Interferon-gamma