title: Delineation of genetic relatedness and population structure of oral and enteric Campylobacter concisus strains by analysis of housekeeping genes.
abstract: Campylobacter concisus is an oral bacterium that has been shown to be associated with inflammatory bowel disease (IBD). In this study we examined clusters of oral C. concisus strains isolated from patients with IBD and healthy controls by analysing six housekeeping genes. In addition, we investigated the population structure of C. concisus strains. Whether oral and enteric strains form distinct clusters based on the sequences of these housekeeping genes was also investigated. The oral C. concisus strains were found to contain two genomospecies, which belong to the two genomospecies previously found in enteric C. concisus strains. C. concisus clusters formed based on the sequences of a single aspA gene were the same as that formed by using previously reported MLST schemes. The analysis of combined oral and enteric C. concisus strains found that enteric C. concisus strains did not form distinct clusters. Genetic structure analysis identified five subpopulations of C. concisus and showed that genetic recombination between C. concisus strains was common. However, genetic recombination was significantly less in oral strains isolated from patients with IBD than from healthy individuals. Previously reported oral and enteric intestinal epithelial invasive C. concisus strains were in cluster II and subpopulation III. Furthermore, this study shows that there are no distinct enteric C. concisus strain clusters or subpopulations.
authors.affiliation: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia.
authors.forename: Vikneswari
authors.initials: V
authors.lastname: Mahendran
authors.affiliation: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia.
authors.forename: Sophie
authors.initials: S
authors.lastname: Octavia
authors.affiliation: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia.
authors.forename: Omer Faruk
authors.initials: OF
authors.lastname: Demirbas
authors.affiliation: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia.
authors.forename: Sheryl
authors.initials: S
authors.lastname: Sabrina
authors.affiliation: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia.
authors.forename: Rena
authors.initials: R
authors.lastname: Ma
authors.affiliation: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia.
authors.forename: Ruiting
authors.initials: R
authors.lastname: Lan
authors.affiliation: Gastrointestinal and Liver Unit, Prince of Wales Hospital, Sydney, Australia.
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Riordan
authors.affiliation: St George and Sutherland Clinical School, University of New South Wales, Sydney, Australia.
authors.forename: Michael C
authors.initials: MC
authors.lastname: Grimm
authors.affiliation: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002167:Campylobacter / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D002169:Campylobacter Infections / Q000382:microbiology*; D016022:Case-Control Studies; D003967:Diarrhea / Q000382:microbiology; D005260:Female; D020043:Genes, Essential; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000382:microbiology*; D008297:Male; D008969:Molecular Sequence Data; D009055:Mouth / Q000382:microbiology*; D058885:Multilocus Sequence Typing; D010802:Phylogeny
chemical_list: D001426:Bacterial Proteins