title: MicroRNA expression profiles in muscle-invasive bladder cancer: identification of a four-microRNA signature associated with patient survival.
abstract: Bladder cancer ranks the second most common genitourinary tract cancer, and muscle-invasive bladder cancer (MIBC) accounts for approximately 25 % of all bladder cancer cases with high mortality. In the current study, with a total of 202 treatment-naïve primary MIBC patients identified from The Cancer Genome Atlas dataset, we comprehensively analyzed the genome-wide microRNA (miRNA) expression profiles in MIBC, with the aim to investigate the relationship of miRNA expression with the progression and prognosis of MIBC, and generate a miRNA signature of prognostic capabilities. In the progression-related miRNA profiles, a total of 47, 16, 3, and 84 miRNAs were selected for pathologic T, N, M, and histologic grade, respectively. Of the eight most important progression-related miRNAs, four (let-7c, mir-125b-1, mir-193a, and mir-99a) were significantly associated with survival of patients with MIBC. Finally, a four-miRNA signature was generated and proven as a promising prognostic parameter. In summary, this study identified the specific miRNAs associated with the progression and aggressiveness of MIBC and a four-miRNA signature as a promising prognostic parameter of MIBC.
authors.affiliation: Department of Urology, Nanjing First Hospital, Nanjing Medical University, 68 Changle Road, Nanjing, 210006, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of Gastroenterology, The Affiliated Jiangning Hospital of Nanjing Medical University, 168 Gushan Road, Nanjing, 211100, China.
authors.forename: Yan-Qiu
authors.initials: YQ
authors.lastname: Yu
authors.affiliation: Department of Urology, Nanjing First Hospital, Nanjing Medical University, 68 Changle Road, Nanjing, 210006, China.
authors.forename: Yu-Zheng
authors.initials: YZ
authors.lastname: Ge
authors.affiliation: Department of Urology, Nanjing First Hospital, Nanjing Medical University, 68 Changle Road, Nanjing, 210006, China.
authors.forename: Jia-Geng
authors.initials: JG
authors.lastname: Zhu
authors.affiliation: Department of Epidemiology and Biostatistics and Ministry of Education Key Laboratory for Modern Toxicology, School of Public Health, Nanjing Medical University, 140 Hanzhong Road, Nanjing, 210029, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Department of Pathology, Nanjing First Hospital, Nanjing Medical University, 68 Changle Road, Nanjing, 210006, China.
authors.forename: You-Cai
authors.initials: YC
authors.lastname: Zhao
authors.affiliation: Department of Urology, Nanjing First Hospital, Nanjing Medical University, 68 Changle Road, Nanjing, 210006, China.
authors.forename: Lu-Wei
authors.initials: LW
authors.lastname: Xu
authors.affiliation: Department of Pathology, Nanjing First Hospital, Nanjing Medical University, 68 Changle Road, Nanjing, 210006, China.
authors.forename: Xiao-Bing
authors.initials: XB
authors.lastname: Yang
authors.affiliation: Department of Epidemiology and Biostatistics and Ministry of Education Key Laboratory for Modern Toxicology, School of Public Health, Nanjing Medical University, 140 Hanzhong Road, Nanjing, 210029, China.
authors.forename: Li-Guo
authors.initials: LG
authors.lastname: Geng
authors.affiliation: Department of Urology, Nanjing First Hospital, Nanjing Medical University, 68 Changle Road, Nanjing, 210006, China.
authors.forename: Quan-Liang
authors.initials: QL
authors.lastname: Dou
authors.affiliation: Department of Urology, Nanjing First Hospital, Nanjing Medical University, 68 Changle Road, Nanjing, 210006, China. urojiarp@126.com.
authors.forename: Rui-Peng
authors.initials: RP
authors.lastname: Jia
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics*; D002291:Carcinoma, Papillary / Q000235:genetics / Q000401:mortality / Q000473:pathology; D018450:Disease Progression; D005260:Female; D005500:Follow-Up Studies; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D019042:Muscle Neoplasms / Q000235:genetics* / Q000401:mortality* / Q000473:pathology; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D011379:Prognosis; D015996:Survival Rate; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000401:mortality* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs
keywords: Biomarker; Muscle-invasive bladder cancer; Prognosis; microRNA