title: The inhibitory effects of a monoclonal antibody targeting neuropilin-1 on adhesion of glioma cells to fibronectin.
abstract: We investigated the inhibitory effects of anti-Neuropilin-1 monoclonal antibody (NRP-1 MAb) on adhesion of glioma cells to Fibronectin (FN) and the associated mechanisms. The effects of NRP-1 MAb on adhesion of U251 glioma cells and formation of stress fibers were studied using adhesion assays and confocal fluorescence microscopy. After treatment of U251 glioma cells with NRP-1 MAb, changes of related proteins adhering to FN were detected by co-immunoprecipitation and Western blot. Adhesion assays showed that NRP-1 MAb played an inhibitory role in adhesion of U251 glioma cells. Confocal fluorescence microscopy, co-immunoprecipitation and Western blot showed that NRP-1 MAb can inhibit the formation of stress fibers adhering to U251 cells. NRP-1 MAb can also affect the formation of Integrin α5β1-NRP-1 complex, and also reduce the level of phosphorylation of FAK and p130cas. In conclusion, NRP-1 MAb has the inhibitory effects on adhesion of glioma cells to FN. This effect is related to the inhibition of NRP-1 MAb on the formation of Integrin α5β1-NRP-1 complex, which leads to the inhibition of FAK/p130cas signaling pathway.
authors.forename: Lukui
authors.initials: L
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Miao
authors.forename: Haipeng
authors.initials: H
authors.lastname: Zhang
authors.forename: Fanwei
authors.initials: F
authors.lastname: Zeng
authors.forename: Chang
authors.initials: C
authors.lastname: Cao
authors.forename: Rong
authors.initials: R
authors.lastname: Qiu
authors.forename: Jinqiu
authors.initials: J
authors.lastname: Yang
authors.forename: Fanghong
authors.initials: F
authors.lastname: Luo
authors.forename: Jianghua
authors.initials: J
authors.lastname: Yan
authors.forename: Heli
authors.initials: H
authors.lastname: Lv
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xu
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects / Q000276:immunology*; D045744:Cell Line, Tumor; D005353:Fibronectins / Q000276:immunology*; D005910:Glioma / Q000276:immunology* / Q000473:pathology*; D006801:Humans; D039942:Neuropilin-1 / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D005353:Fibronectins; D039942:Neuropilin-1