title: Evaluation of an indirect ELISA using recombinant granule antigen GRA1, GRA7 and soluble antigens for serodiagnosis of Toxoplasma gondii infection in chickens.
abstract: In the present study, recombinant granule antigen proteins GRA1, GRA7 and Toxoplasma soluble antigens (TSA) were evaluated as potential diagnostic markers for T. gondii infection in chickens by an indirect enzyme-linked immunosorbent assay (ELISA), showing a seroprevalence of 16.4% by GRA1-ELISA, and 15.5% by both GRA7- and TSA-ELISA in chickens. No significant difference was observed in the inconsistent results (P > 0.05), and a substantial agreement was found in the consistent results of the 3 tests (92.7-97.3%). Compared with the reference test Western blot, GRA7-ELISA showed the highest co-positivity and co-negativity rates. Receiver operating characteristic (ROC) analysis revealed a largest area under curve (AUC) of 0.99 (95% CI, 98-1.0), and a highest relative sensitivity (100%) and specificity (97.9%) for a cut-off value of 0.34 in GRA7-ELISA. These results of the present study showed that GRA7 is a potential diagnostic marker for the detection of T. gondii infection in chickens.
authors.affiliation: College of Life Science, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, Jilin Province, China.
authors.forename: Xuefei
authors.initials: X
authors.lastname: Sun
authors.affiliation: Military Veterinary Institute, Academy of Military Medical Sciences, Changchun 130122, Jilin Province, China.
authors.forename: Zedong
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Life Science, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, Jilin Province, China.
authors.forename: Jiping
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Life Science, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, Jilin Province, China. Electronic address: jlccwf@126.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Wei
authors.affiliation: Military Veterinary Institute, Academy of Military Medical Sciences, Changchun 130122, Jilin Province, China. Electronic address: liuquan1974@hotmail.com.
authors.forename: Quan
authors.initials: Q
authors.lastname: Liu
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D011201:Poultry Diseases / Q000175:diagnosis* / Q000469:parasitology; D015800:Protozoan Proteins; D012372:ROC Curve; D011994:Recombinant Proteins; D012680:Sensitivity and Specificity; D012698:Serologic Tests; D014122:Toxoplasma / Q000302:isolation & purification*; D014124:Toxoplasmosis, Animal / Q000175:diagnosis* / Q000469:parasitology
chemical_list: D000953:Antigens, Protozoan; C412913:GRA1 antigen; C112573:GRA7 protein, Toxoplasma gondii; D015800:Protozoan Proteins; D011994:Recombinant Proteins
keywords: Chickens; Diagnostic marker; ELISA; GRA1; GRA7; Toxoplasma gondii