title: HS-4, a highly potent inhibitor of cell proliferation of human cancer cell.
abstract: OBJECTIVE
  To investigate the antitumor activity of the compound HS-4 and the action mechanism.
  
  
  METHODS
  MTT method was used to test in vitro antitumor activity of the compound HS-4. Orthotopic xenotransplantation tumor model of liver cancer was established in nude mice, and, in vivo antitumor activity of compound HS-4 was tested with a small animal in-vivo imaging system. Sequencing of small RNA library and RNA library was performed in HS-4 treated tumor cell group and control group to investigate the anti-cancer mechanism of HS-4 at level of functional genomics, using high-throughput sequencing technology.
  
  
  RESULTS
  HS-4 was found to have relatively high in-vitro antitumor activity against liver cancer cells, gastric cancer cells, renal cancer cells, lung cancer cells, breast cancer cells and colon cancer cells. The IC50 values against SMMC-7721 and Bel-7402 of liver cancer cells were 0.14 and 0.13 nmol/L respectively, while the IC50 values against MGC-803 and SGC-7901 of gastric cancer cells were 0.19 and 0.21 nmol/L, respectively. It was demonstrated that HS-4 possessed a better therapeutic effect in liver cancer.
  
  
  CONCLUSIONS
  A new reliable orthotopic xenotransplantation tumor model of liver cancer in nude mice is established. The new compounds HS-4 was found to possess relatively high in vivo and in vitro antitumor activity against liver cancer cells.
authors.affiliation: Hainan Province Drug Safety Evaluation and Research Center, Hainan Medical College, Haikou, 571199, China.
authors.forename: Gui-Lan
authors.initials: GL
authors.lastname: Xing
authors.affiliation: Hainan Province Drug Safety Evaluation and Research Center, Hainan Medical College, Haikou, 571199, China.
authors.forename: Shu-Hong
authors.initials: SH
authors.lastname: Tian
authors.affiliation: Hainan Province Drug Safety Evaluation and Research Center, Hainan Medical College, Haikou, 571199, China. Electronic address: xie_xueli@126.com.
authors.forename: Xue-Li
authors.initials: XL
authors.lastname: Xie
authors.affiliation: Hainan Province Drug Safety Evaluation and Research Center, Hainan Medical College, Haikou, 571199, China. Electronic address: Fujian.hnmc@163.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Fu
keywords: Antitumor; BeL-7402 cells; HS-4; In vivo; Orthotopic xenotransplantation