title: Clinical significance of miR-140-5p and miR-193b expression in patients with breast cancer and relationship to IGFBP5.
abstract: The functional role of IGFBP5 in breast cancer is complicated. Experimental and bioinformatics studies have shown that IGFBP5 is targeted by miR-140-5p and miR-193b, although this has not yet been proven in clinical samples. The aim of this study was to evaluate the expression of miR-140-5p and miR-193b in breast cancer and adjacent normal tissue and assess its correlation with IGFBP5 and the clinicopathological characteristics of the tumors. IGFBP5 protein expression was analyzed immunohistochemically and IGFBP5, miR-140 and miR-193b mRNA expression levels were analyzed with real-time RT-PCR. Tumor tissue had higher miR-140-5p expression than adjacent normal tissue (p = 0.015). Samples with no immunohistochemical staining for IGFBP5 showed increased miR-140-5p expression (p = 0.009). miR-140-5p expression was elevated in invasive ductal carcinomas (p = 0.002), whereas basal-like tumors had decreased expression of miR-140-5p compared to other tumors (p = 0.008). Lymph node-positive samples showed an approximately 13-fold increase in miR-140-5p expression compared to lymph node-negative tissue (p = 0.049). These findings suggest that miR-140-5p, but not miR-193b, could be an important determinant of IGFBP5 expression and clinical phenotype in breast cancer patients. Further studies are needed to clarify the expressional regulation of IGFBP5 by miR-140-5p.
authors.affiliation: Department of Medical Biology, School of Medicine, Marmara University, Istanbul, Turkey .
authors.forename: Gökçe
authors.initials: G
authors.lastname: Güllü
authors.affiliation: Department of Medical Biology, School of Medicine, Marmara University, Istanbul, Turkey .
authors.forename: Irem
authors.initials: I
authors.lastname: Peker
authors.affiliation: Department of Pathology, Haydarpasa Training Hospital, Gülhane Military Medical Academy, Istanbul, Turkey .
authors.forename: Aptullah
authors.initials: A
authors.lastname: Haholu
authors.affiliation: Department of Medical Biology, School of Medicine, Marmara University, Istanbul, Turkey .
authors.forename: Fatih
authors.initials: F
authors.lastname: Eren
authors.affiliation: Department of Pathology, Haydarpasa Training Hospital, Gülhane Military Medical Academy, Istanbul, Turkey .
authors.forename: Zafer
authors.initials: Z
authors.lastname: Küçükodaci
authors.affiliation: Department of General Surgery, Haydarpasa Training Hospital, Gülhane Military Medical Academy, Istanbul, Turkey .
authors.forename: Bülent
authors.initials: B
authors.lastname: Güleç
authors.affiliation: Department of Pathology, Anadolu Medical Center, Istanbul, Turkey .
authors.forename: Hüseyin
authors.initials: H
authors.lastname: Baloglu
authors.affiliation: Department of Medical Biology, School of Medicine, Marmara University, Istanbul, Turkey .
authors.forename: Can
authors.initials: C
authors.lastname: Erzik
authors.affiliation: Department of Medical Biology, School of Medicine, Marmara University, Istanbul, Turkey .
authors.forename: Ayse
authors.initials: A
authors.lastname: Özer
authors.affiliation: Department of Medical Biology, School of Medicine, Marmara University, Istanbul, Turkey .
authors.forename: Mustafa
authors.initials: M
authors.lastname: Akkiprik
keywords: ER alpha; IGFBP5; breast cancer; miR-140; miR-193b; micro RNA