title: Metagenomics Approaches Based on Virion-Associated Nucleic Acids (VANA): An Innovative Tool for Assessing Without A Priori Viral Diversity of Plants.
abstract: This chapter describes an efficient approach that combines quality and yield extraction of viral nucleic acids from plants containing high levels of secondary metabolites and a sequence-independent amplification procedure for both the inventory of known plant viruses and the discovery of unknown ones. This approach turns out to be a useful tool for assessing the virome (the genome of all the viruses that inhabit a particular organism) of plants of interest. We here show that this approach enables the identification of a novel Potyvirus member within a single plant already known to be infected by two other Potyvirus species.
authors.affiliation: Cirad-INRA-Montpellier SupAgro, UMR BGPI, TA A-54/K, Campus International de Montferrier-Baillarguet, Montpellier Cedex 5, 34398, France.
authors.forename: Denis
authors.initials: D
authors.lastname: Filloux
authors.forename: Sylvie
authors.initials: S
authors.lastname: Dallot
authors.forename: Agnès
authors.initials: A
authors.lastname: Delaunay
authors.forename: Serge
authors.initials: S
authors.lastname: Galzi
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Jacquot
authors.forename: Philippe
authors.initials: P
authors.lastname: Roumagnac
mesh_terms: D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D027742:Dioscorea / Q000821:virology*; D016679:Genome, Viral; D056186:Metagenomics; D010935:Plant Diseases / Q000821:virology; D010942:Plant Viruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012367:RNA, Viral / Q000032:analysis* / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity; D014771:Virion / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral