title: TGF-β induced miR-132 enhances the activation of TGF-β signaling through inhibiting SMAD7 expression in glioma cells.
abstract: Transforming growth factors β (TGF-β) pathway has been proven to play important roles in oncogenesis and angiogenesis of gliomas. MiR-132 might be related to TGF-β signaling pathway and high miR-132 expression was reported to be a biomarker of poor prognosis in patients diagnosed with glioma. However, the expression regulation way involved in TGF-β pathway and clinical significance of miR-132 have not been investigated in glioma cells. Here we reported that the mRNA level of miR-132 and TGF-β concentration were both increased in patients with brain glioma. Correlation analysis revealed that TGF-β concentration was positively correlated with mRNA level of miR-132. In addition, the mRNA level of miR-132 was up-regulated by TGF-β in a concentration-dependent and time-dependent manner. Furthermore, we found that miR-132 was involved in modulation of the TGF-β signaling pathway and down-regulation of SMAD7 expression by directly targeting the SMAD7 3'-UTR. MiR-132 was negatively correlated with SMAD7 in patients with brain glioma. Taken together, our results suggest that miR-132 could be stimulated by TGF-β and might enhance the activation of TGF-β signaling through inhibiting SMAD7 expression in glioma cells. These findings contribute to a better understanding of the mechanism of the activation of TGF-β signaling by miR-132.
authors.affiliation: Department of Internal Neurology, Huaihe Hospital of Henan University, Kaifeng, 475000, China. Electronic address: wzhkf0526@163.com.
authors.forename: Zhao-hui
authors.initials: ZH
authors.lastname: Wang
authors.affiliation: Department of Internal Neurology, Huaihe Hospital of Henan University, Kaifeng, 475000, China.
authors.forename: Qi-shun
authors.initials: QS
authors.lastname: Zhang
authors.affiliation: Department of Ultrasonography, Obstetrics and Gynecology Hospital of Kaifeng, Kaifeng, 475000, China.
authors.forename: Yan-li
authors.initials: YL
authors.lastname: Duan
authors.affiliation: Department of Internal Neurology, Huaihe Hospital of Henan University, Kaifeng, 475000, China.
authors.forename: Jian-lei
authors.initials: JL
authors.lastname: Zhang
authors.affiliation: Department of Internal Neurology, Huaihe Hospital of Henan University, Kaifeng, 475000, China.
authors.forename: Guo-fei
authors.initials: GF
authors.lastname: Li
authors.affiliation: Department of Internal Neurology, Huaihe Hospital of Henan University, Kaifeng, 475000, China.
authors.forename: Dong-lin
authors.initials: DL
authors.lastname: Zheng
mesh_terms: D001921:Brain / Q000378:metabolism; D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D051906:Smad7 Protein / Q000235:genetics*; D016212:Transforming Growth Factor beta / Q000378:metabolism*
chemical_list: C547077:MIRN132 microRNA, human; D035683:MicroRNAs; D051906:Smad7 Protein; D016212:Transforming Growth Factor beta
keywords: Glioma cells; SMAD2; SMAD7; Transforming growth factors β; miR-132