title: Antigenotoxic and antimutagenic effects of diphenyl ditelluride against several known mutagens in Chinese hamster lung fibroblasts.
abstract: The present study evaluates antigenotoxic and antimutagenic properties of diphenyl ditelluride (DPDT) against several known mutagens in Chinese hamster lung fibroblasts (V79 cells). DPDT was not cytotoxic and genotoxic at concentrations ranging from 0.01 to 0.1 μM. The pre-treatment for 2h with this organotellurium compound at non-cytotoxic dose range (0.01, 0.05 and 0.1 μM) increased cell survival after challenge with hydrogen peroxide (H2O2), t-butyl hydroperoxide (t-BOOH), methylmethanesulphonate (MMS) or ultraviolet (UV)C radiation. In addition, the pre-treatment with DPDT decreased the DNA damage and Formamidopyrimidine DNA-glycosylase (Fpg)- and Endonuclease III (Endo III) sensitive sites induction by the studied genotoxic agents, as verified by comet assay and modified comet assay, respectively. The pre-treatment also reduced micronucleus frequency, revealing the protector effect of DPDT against MMS and UVC-induced mutagenesis. Our results demonstrate that DPDT-treated cells at concentration range of 0.01-0.1 μM do not change thiobarbituric acid reactive species (TBARS) levels and ROS generation. Moreover, DPDT pre-treatment at this concentration range decreases the ROS induction by H2O2 and t-BOOH treatment indicating antioxidant potential. On the other hand, concentrations higher than 0.1 μM increase TBARS formation and inhibited superoxide dismutase (SOD) activity, suggesting pro-oxidative effect of this compound at high concentrations. Our results suggest that DPDT presents antigenotoxic and antimutagenic properties at concentration range of 0.01-0.1 μM. The protection effect could be attributed to antioxidant capacity of DPDT at this concentration range in V79 cells.
authors.affiliation: Department of Biophysics, Federal University of Rio Grande do Sul - UFRGS, Porto Alegre - RS, Brazil.
authors.forename: Cristiano
authors.initials: C
authors.lastname: Trindade
authors.affiliation: Department of Biophysics, Federal University of Rio Grande do Sul - UFRGS, Porto Alegre - RS, Brazil.
authors.forename: André L M
authors.initials: AL
authors.lastname: Juchem
authors.affiliation: Department of Biophysics, Federal University of Rio Grande do Sul - UFRGS, Porto Alegre - RS, Brazil.
authors.forename: Nathália R M
authors.initials: NR
authors.lastname: de Albuquerque
authors.affiliation: Department of Biophysics, Federal University of Rio Grande do Sul - UFRGS, Porto Alegre - RS, Brazil.
authors.forename: Iuri M
authors.initials: IM
authors.lastname: de Oliveira
authors.affiliation: Department of Biophysics, Federal University of Rio Grande do Sul - UFRGS, Porto Alegre - RS, Brazil.
authors.forename: Renato M
authors.initials: RM
authors.lastname: Rosa
authors.affiliation: Department of Biophysics, Federal University of Rio Grande do Sul - UFRGS, Porto Alegre - RS, Brazil.
authors.forename: Temenouga N
authors.initials: TN
authors.lastname: Guecheva
authors.affiliation: Department of Basic Health Sciences, Federal University of Health Sciences of Porto Alegre - UFCSPA, Porto Alegre - RS, Brazil and.
authors.forename: Jenifer
authors.initials: J
authors.lastname: Saffi
authors.affiliation: Department of Biophysics, Federal University of Rio Grande do Sul - UFRGS, Porto Alegre - RS, Brazil, Institute of Biotechnology, University of Caxias do Sul - UCS, Caxias do Sul - RS, Brazil pegas@cbiot.ufrgs.br.
authors.forename: João A P
authors.initials: JA
authors.lastname: Henriques
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D001555:Benzene Derivatives / Q000494:pharmacology*; D015415:Biomarkers; D002374:Catalase / Q000378:metabolism; D002460:Cell Line; D020552:Comet Assay; D006224:Cricetinae; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008168:Lung; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced; D009152:Mutagenicity Tests; D009153:Mutagens / Q000494:pharmacology*; D009942:Organometallic Compounds / Q000494:pharmacology*; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D016587:Antimutagenic Agents; D001555:Benzene Derivatives; D015415:Biomarkers; D009153:Mutagens; D009942:Organometallic Compounds; D017382:Reactive Oxygen Species; C434247:diphenylditelluride; D007770:L-Lactate Dehydrogenase; D002374:Catalase; D013482:Superoxide Dismutase