title: Nanomanufacturing of Tobacco Mosaic Virus-Based Spherical Biomaterials Using a Continuous Flow Method.
abstract: Nanomanufacturing of nanoparticles is critical for potential translation and commercialization. Continuous flow devices can alleviate this need through unceasing production of nanoparticles. Here we demonstrate the scaled-up production of spherical nanoparticles functionalized with biomedical cargos from the rod-shaped plant virus tobacco mosaic virus (TMV) using a mesofluidic, continued flow method. Production yields were increased 30-fold comparing the mesofluidic device versus batch methods. Finally, we produced MRI contrast agents of select sizes, with per particle relaxivity reaching 979,218 mM-1s-1at 60 MHz. These TMV-based spherical nanoparticle MRI contrast agents are in the top echelon of relaxivity per nanoparticle.
authors.affiliation: Department of Biomedical Engineering, Department of Radiology, Department of Materials Science and Engineering, and Department of Macromolecular Engineering, Case Western Reserve University Schools of Medicine and Engineering , 10900 Euclid Avenue, Cleveland, Ohio 44106, United States.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Bruckman
authors.affiliation: Department of Biomedical Engineering, Department of Radiology, Department of Materials Science and Engineering, and Department of Macromolecular Engineering, Case Western Reserve University Schools of Medicine and Engineering , 10900 Euclid Avenue, Cleveland, Ohio 44106, United States.
authors.forename: Allen
authors.initials: A
authors.lastname: VanMeter
authors.affiliation: Department of Biomedical Engineering, Department of Radiology, Department of Materials Science and Engineering, and Department of Macromolecular Engineering, Case Western Reserve University Schools of Medicine and Engineering , 10900 Euclid Avenue, Cleveland, Ohio 44106, United States.
authors.forename: Nicole F
authors.initials: NF
authors.lastname: Steinmetz
keywords: MRI contrast agents; mesofluidic device; nanomanufacturing; nanoparticle shape; tobacco mosaic virus