title: Spatial Compartmentalization Specializes the Function of Aurora A and Aurora B.
abstract: Aurora kinase A and B share great similarity in sequences, structures, and phosphorylation motif, yet they show different localizations and play distinct crucial roles. The factors that determine such differences are largely unknown. Here we targeted Aurora A to the localization of Aurora B and found that Aurora A phosphorylates the substrate of Aurora B and substitutes its function in spindle checkpoint. In return, the centrosome targeting of Aurora B substitutes the function of Aurora A in the mitotic entry. Expressing the chimera proteins of the Auroras with exchanged N termini in cells indicates that the divergent N termini are also important for their spatiotemporal localizations and functions. Collectively, we demonstrate that functional divergence of Aurora kinases is determined by spatial compartmentalization, and their divergent N termini also contribute to their spatial and functional differentiation.
authors.affiliation: From the Ministry of Education Key Laboratory of Bio-resources and Eco-environment, College of Life Sciences, Sichuan University, Chengdu, Sichuan 610064 and Ministry of Education Key Laboratory of Cell Proliferation and Differentiation and State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Si
authors.initials: S
authors.lastname: Li
authors.affiliation: Ministry of Education Key Laboratory of Cell Proliferation and Differentiation and State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Zhaoxuan
authors.initials: Z
authors.lastname: Deng
authors.affiliation: Ministry of Education Key Laboratory of Cell Proliferation and Differentiation and State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Jingyan
authors.initials: J
authors.lastname: Fu
authors.affiliation: Ministry of Education Key Laboratory of Cell Proliferation and Differentiation and State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Caiyue
authors.initials: C
authors.lastname: Xu
authors.affiliation: Ministry of Education Key Laboratory of Cell Proliferation and Differentiation and State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Guangwei
authors.initials: G
authors.lastname: Xin
authors.affiliation: Ministry of Education Key Laboratory of Cell Proliferation and Differentiation and State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Zhige
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Ministry of Education Key Laboratory of Cell Proliferation and Differentiation and State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Luo
authors.affiliation: Ministry of Education Key Laboratory of Cell Proliferation and Differentiation and State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Wang
authors.affiliation: Ministry of Education Key Laboratory of Cell Proliferation and Differentiation and State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Shuli
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Ministry of Education Key Laboratory of Cell Proliferation and Differentiation and State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Boyan
authors.initials: B
authors.lastname: Zhang
authors.affiliation: From the Ministry of Education Key Laboratory of Bio-resources and Eco-environment, College of Life Sciences, Sichuan University, Chengdu, Sichuan 610064 and fundzou@scu.edu.cn.
authors.forename: Fangdong
authors.initials: F
authors.lastname: Zou
authors.affiliation: Ministry of Education Key Laboratory of Cell Proliferation and Differentiation and State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing 100871, China jiangqing@pku.edu.cn.
authors.forename: Qing
authors.initials: Q
authors.lastname: Jiang
authors.affiliation: Ministry of Education Key Laboratory of Cell Proliferation and Differentiation and State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing 100871, China zhangcm@pku.edu.cn.
authors.forename: Chuanmao
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D064096:Aurora Kinase A / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D064107:Aurora Kinase B / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002451:Cell Compartmentation; D059447:Cell Cycle Checkpoints; D018385:Centrosome / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D019143:Evolution, Molecular; D006367:HeLa Cells; D006657:Histones / Q000378:metabolism; D006801:Humans; D018386:Kinetochores / Q000378:metabolism; D008938:Mitosis; D008954:Models, Biological; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D008941:Spindle Apparatus / Q000378:metabolism
chemical_list: D002843:Chromatin; D006657:Histones; D011993:Recombinant Fusion Proteins; C576917:AURKA protein, human; C576921:AURKB protein, human; D064096:Aurora Kinase A; D064107:Aurora Kinase B
keywords: Aurora kinase; INCENP; TPX2; cell cycle; checkpoint control; mitotic spindle; protein kinase; protein phosphorylation; spindle assembly.