title: Correlation between Either Cupriavidus or Porphyromonas and Primary Pulmonary Tuberculosis Found by Analysing the Microbiota in Patients' Bronchoalveolar Lavage Fluid.
abstract: Pulmonary tuberculosis (TB) has gained attention in recent decades because of its rising incidence trend; simultaneously, increasing numbers of studies have identified the relationship between microbiota and chronic infectious diseases. In our work, we enrolled 32 patients with primary TB characterised by unilateral TB lesion formation diagnosed by chest radiographic exam. Bronchoalveolar lavage fluid was taken from both lungs. Twenty-four healthy people were chosen as controls. Pyrosequencing was performed on the V3 hypervariable region of 16S rDNA in all bacterial samples and used as a culture-independent method to describe the phylogenetic composition of the microbiota. Through pyrosequencing, 271,764 amplicons were detected in samples and analysed using tools in the Ribosomal Database Project (RDP) and bioinformatics. These analyses revealed significant differences in the microbiota in the lower respiratory tract (LRT) of TB patients compared with healthy controls; in contrast, the microbiota of intra/extra-TB lesions were similar. These results showed that the dominant bacterial genus in the LRT of TB patients was Cupriavidus and not Streptococcus, which resulted in a significant change in the microbiota in TB patients. The abundance of Mycobacteria and Porphyromonas significantly increased inside TB lesions when compared with non-lesion-containing contralateral lungs. From these data, it can be concluded that Cupriavidus plays an important role in TB's secondary infection and that in addition to Mycobacteria, Porphyromonas may also be a co-factor in lesion formation. The mechanisms underlying this connection warrant further research.
authors.affiliation: Department of Medical Microbiology and Parasitology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Department of Emergency Medicine, Rui Jin Hospital Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Tuberculosis, Nanjing Chest Hospital, Nanjing, Jiangsu, China.
authors.forename: Feishen
authors.initials: F
authors.lastname: Lin
authors.affiliation: Department of Medical Microbiology and Parasitology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Zelin
authors.initials: Z
authors.lastname: Cui
authors.affiliation: Department of Tuberculosis, Nanjing Chest Hospital, Nanjing, Jiangsu, China.
authors.forename: Xiangrong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Tuberculosis, Nanjing Chest Hospital, Nanjing, Jiangsu, China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Hu
authors.affiliation: Department of Preventive Medicine, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Shen
authors.affiliation: Department of Medical Microbiology and Parasitology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Tuberculosis, Nanjing Chest Hospital, Nanjing, Jiangsu, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Medical Microbiology and Parasitology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Xiaokui
authors.initials: X
authors.lastname: Guo
mesh_terms: D000293:Adolescent; D000328:Adult; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology*; D049921:Cupriavidus / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D064307:Microbiota / Q000502:physiology*; D008875:Middle Aged; D017861:Porphyromonas / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D014397:Tuberculosis, Pulmonary / Q000382:microbiology*; D055815:Young Adult
chemical_list: D004269:DNA, Bacterial