title: Designed Intercalators for Modification of DNA Origami Surface Properties.
abstract: The modification of the backbone properties of DNA origami nanostructures through noncovalent interactions with designed intercalators, based on acridine derivatized with side chains containing esterified fatty acids or oligo(ethylene glycol) residues is reported. Spectroscopic analyses indicate that these intercalators bind to DNA origami structures. Atomic force microscopy studies reveal that intercalator binding does not affect the structural intactness but leads to altered surface properties of the highly negatively charged nanostructures, as demonstrated by their interaction with solid mica or graphite supports. Moreover, the noncovalent interaction between the intercalators and the origami structures leads to alteration in cellular uptake, as shown by confocal microscopy studies using two different eukaryotic cell lines. Hence, the intercalator approach offers a potential means for tailoring the surface properties of DNA nanostructures.
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute for Biological Interfaces (IBG 1), Hermann-von-Helmholtz-Platz, 76344 Eggenstein-Leopoldshafen (Germany).
authors.forename: Josipa
authors.initials: J
authors.lastname: Brglez
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute for Biological Interfaces (IBG 1), Hermann-von-Helmholtz-Platz, 76344 Eggenstein-Leopoldshafen (Germany).
authors.forename: Pavel
authors.initials: P
authors.lastname: Nikolov
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute for Biological Interfaces (IBG 1), Hermann-von-Helmholtz-Platz, 76344 Eggenstein-Leopoldshafen (Germany).
authors.forename: Alessandro
authors.initials: A
authors.lastname: Angelin
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute for Biological Interfaces (IBG 1), Hermann-von-Helmholtz-Platz, 76344 Eggenstein-Leopoldshafen (Germany). niemeyer@kit.edu.
authors.forename: Christof M
authors.initials: CM
authors.lastname: Niemeyer
mesh_terms: D004247:DNA / Q000737:chemistry*; D004951:Esterification; D019855:Ethylene Glycol / Q000737:chemistry; D005057:Eukaryotic Cells / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D006801:Humans; D007364:Intercalating Agents / Q000138:chemical synthesis* / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry*; D009696:Nucleic Acids / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D005227:Fatty Acids; D007364:Intercalating Agents; D009696:Nucleic Acids; D004247:DNA; D019855:Ethylene Glycol
keywords: DNA nanostructures; intercalators; nucleic acids; self-assembly; surface binding