title: The transcriptomic response to copper exposure in the digestive gland of Japanese scallops (Mizuhopecten yessoensis).
abstract: The present study was conducted to elucidate the effects of copper exposure on the immune system and lipid metabolism of the Japanese scallop, Mizuhopecten yessoensis. Transcriptional levels of differentially expressed genes (DEGs)in M. yessoensis digestive gland tissue were analyzed using the deep-sequencing platform Illumina HiSeq™ 2000. In total, 841 and 877 genes were identified as significantly up- or down-regulated, respectively. In addition, significant enrichment analysis identified 3 gene ontology terms and 15 pathways involved in the response to copper exposure. Analysis of transcripts related to the immune response revealed a complex pattern of innate recognition receptors, including toll-like receptors, NOD-like receptors and downstream pathway effectors, including those involved in apoptosis. Furthermore, genomic analysis revealed that genes involved in extracellular matrix (ECM)-receptor interactions were enriched in Cu-exposed scallop glands. These results will provide a resource for subsequent gene expression studies regarding heavy metal exposure and the identification of copper-sensitive biomarkers for the aquaculture of M. yessoensis.
authors.affiliation: College of Fisheries, Henan Normal University, Xinxiang 453007, PR China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Meng
authors.affiliation: College of Fisheries, Henan Normal University, Xinxiang 453007, PR China.
authors.forename: Xue
authors.initials: X
authors.lastname: Tian
authors.affiliation: College of Fisheries, Henan Normal University, Xinxiang 453007, PR China. Electronic address: niegx@htu.cn.
authors.forename: Guoxing
authors.initials: G
authors.lastname: Nie
authors.affiliation: College of Fisheries, Henan Normal University, Xinxiang 453007, PR China.
authors.forename: Junli
authors.initials: J
authors.lastname: Wang
authors.affiliation: R&D Center of Marine, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China.
authors.forename: Mei
authors.initials: M
authors.lastname: Liu
authors.affiliation: R&D Center of Marine, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China.
authors.forename: Keyong
authors.initials: K
authors.lastname: Jiang
authors.affiliation: R&D Center of Marine, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China.
authors.forename: Baojie
authors.initials: B
authors.lastname: Wang
authors.affiliation: College of Fisheries, Henan Normal University, Xinxiang 453007, PR China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Guo
authors.affiliation: College of Fisheries, Henan Normal University, Xinxiang 453007, PR China.
authors.forename: Jianrong
authors.initials: J
authors.lastname: Huang
authors.affiliation: R&D Center of Marine, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China. Electronic address: wanglei@ms.qdio.ac.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
mesh_terms: D000818:Animals; D003300:Copper / Q000633:toxicity*; D004064:Digestive System / Q000187:drug effects; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D059014:High-Throughput Nucleotide Sequencing; D050356:Lipid Metabolism / Q000187:drug effects*; D008297:Male; D009928:Organ Specificity; D049897:Pectinidae / Q000187:drug effects* / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D017422:Sequence Analysis, DNA; D059467:Transcriptome / Q000187:drug effects*; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D014874:Water Pollutants, Chemical; D003300:Copper
keywords: Copper; Deep-sequencing; Digestive gland; Mizuhopecten yessoensis