title: Identification of anti-biofilm components in Withania somnifera and their effect on virulence of Streptococcus mutans biofilms.
abstract: OBJECTIVE
  The aim of this study was to identify components of the Withania somnifera that could show anti-virulence activity against Streptococcus mutans biofilms.
  
  
  RESULTS
  The anti-acidogenic activity of fractions separated from W. somnifera was compared, and then the most active anti-acidogenic fraction was chemically characterized using gas chromatography-mass spectroscopy. The effect of the identified components on the acidogenicity, aciduricity and extracellular polymeric substances (EPS) formation of S. mutans UA159 biofilms was evaluated. The change in accumulation and acidogenicity of S. mutans UA159 biofilms by periodic treatments (10 min per treatment) with the identified components was also investigated. Of the fractions, n-hexane fraction showed the strongest anti-acidogenic activity and was mainly composed of palmitic, linoleic and oleic acids. Of the identified components, linoleic and oleic acids strongly affected the acid production rate, F-ATPase activity and EPS formation of the biofilms. Periodic treatment with linoleic and oleic acids during biofilm formation also inhibited the biofilm accumulation and acid production rate of the biofilms without killing the biofilm bacteria.
  
  
  CONCLUSIONS
  These results suggest that linoleic and oleic acids may be effective agents for restraining virulence of S. mutans biofilms.
  
  
  CONCLUSIONS
  Linoleic and oleic acids may be promising agents for controlling virulence of cariogenic biofilms and subsequent dental caries formation.
authors.affiliation: Department of Preventive Dentistry, School of Dentistry, Institute of Oral Bioscience and BK 21 Plus Program, Chonbuk National University, Jeonju, Korea.
authors.forename: S
authors.initials: S
authors.lastname: Pandit
authors.affiliation: Department of Preventive Dentistry, School of Dentistry, Institute of Oral Bioscience and BK 21 Plus Program, Chonbuk National University, Jeonju, Korea.
authors.forename: J N
authors.initials: JN
authors.lastname: Cai
authors.affiliation: Research Institute of Clinical Medicine of Chonbuk National University, Biomedical Research Institute of Chonbuk National University Hospital, Jeonju, Korea.
authors.forename: K Y
authors.initials: KY
authors.lastname: Song
authors.affiliation: Department of Preventive Dentistry, School of Dentistry, Institute of Oral Bioscience and BK 21 Plus Program, Chonbuk National University, Jeonju, Korea.|Research Institute of Clinical Medicine of Chonbuk National University, Biomedical Research Institute of Chonbuk National University Hospital, Jeonju, Korea.
authors.forename: J G
authors.initials: JG
authors.lastname: Jeon
mesh_terms: D018441:Biofilms / Q000187:drug effects*; D003731:Dental Caries / Q000382:microbiology; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D013290:Streptococcal Infections / Q000382:microbiology*; D013295:Streptococcus mutans / Q000187:drug effects / Q000472:pathogenicity* / Q000502:physiology*; D014774:Virulence / Q000187:drug effects; D032341:Withania / Q000737:chemistry*
chemical_list: D010936:Plant Extracts
keywords: Streptococcus mutans biofilm; Withania somnifera; acidogenicity; aciduricity; dental caries; linoleic acid