title: HIFs genes expression and hematology indices responses to different oxygen treatments in an ovoviviparous teleost species Sebastes schlegelii.
abstract: Hypoxia-inducible factors (HIFs) are transcription factors considered as a respond factor to oxygen tension. By using quantitative real-time PCR, expression files of HIF-1α and HIF-2α mRNA were detected in the Korean rockfish ovary, liver, gill and spleen after 30 min and 60 min acute hypoxia exposure. Meanwhile, the cortisol levels, white blood cells and several serum biochemical values of Korean rockfish under different oxygen concentration treatments were also detected. All the results might be helpful for further understanding of the potential effect of hypoxia in ovoviviparous fish.
authors.affiliation: Fisheries College, Ocean University of China, Qingdao 266003, China; College of Life Science and Technology, Harbin Normal University, Harbin 150025, China.
authors.forename: Weijie
authors.initials: W
authors.lastname: Mu
authors.affiliation: Fisheries College, Ocean University of China, Qingdao 266003, China. Electronic address: wenhaishen@ouc.edu.cn.
authors.forename: Haishen
authors.initials: H
authors.lastname: Wen
authors.affiliation: Fisheries College, Ocean University of China, Qingdao 266003, China.
authors.forename: Jifang
authors.initials: J
authors.lastname: Li
authors.affiliation: Fisheries College, Ocean University of China, Qingdao 266003, China.
authors.forename: Feng
authors.initials: F
authors.lastname: He
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D005399:Fishes / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010100:Oxygen / Q000378:metabolism*; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA; D000099162:Endothelial PAS Domain-Containing Protein 1
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D018076:DNA, Complementary; D029941:Fish Proteins; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D010100:Oxygen; D012333:RNA, Messenger; D000099162:Endothelial PAS Domain-Containing Protein 1
keywords: Aquaculture; Cortisol; Hematology indices; Hypoxia; Real-time-PCR; Tissue analysis; mRNA expression