title: Encouragement of Enzyme Reaction Utilizing Heat Generation from Ferromagnetic Particles Subjected to an AC Magnetic Field.
abstract: We propose a method of activating an enzyme utilizing heat generation from ferromagnetic particles under an ac magnetic field. We immobilize α-amylase on the surface of ferromagnetic particles and analyze its activity. We find that when α-amylase/ferromagnetic particle hybrids, that is, ferromagnetic particles, on which α-amylase molecules are immobilized, are subjected to an ac magnetic field, the particles generate heat and as a result, α-amylase on the particles is heated up and activated. We next prepare a solution, in which α-amylase/ferromagnetic particle hybrids and free, nonimmobilized chitinase are dispersed, and analyze their activities. We find that when the solution is subjected to an ac magnetic field, the activity of α-amylase immobilized on the particles increases, whereas that of free chitinase hardly changes; in other words, only α-amylase immobilized on the particles is selectively activated due to heat generation from the particles.
authors.affiliation: Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Suzuki
authors.affiliation: Bio-Nano Electronics Research Center, Toyo University, Kawagoe, Saitama, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Aki
authors.affiliation: Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama, Japan; Bio-Nano Electronics Research Center, Toyo University, Kawagoe, Saitama, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Mizuki
authors.affiliation: Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama, Japan; Bio-Nano Electronics Research Center, Toyo University, Kawagoe, Saitama, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Maekawa
authors.affiliation: Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama, Japan; Bio-Nano Electronics Research Center, Toyo University, Kawagoe, Saitama, Japan.
authors.forename: Ron
authors.initials: R
authors.lastname: Usami
authors.affiliation: Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama, Japan; Bio-Nano Electronics Research Center, Toyo University, Kawagoe, Saitama, Japan.
authors.forename: Hisao
authors.initials: H
authors.lastname: Morimoto
mesh_terms: D004789:Enzyme Activation; D005296:Ferrous Compounds / Q000737:chemistry*; D006358:Hot Temperature; D008280:Magnetics; D008855:Microscopy, Electron, Scanning; D000516:alpha-Amylases / Q000378:metabolism*
chemical_list: D005296:Ferrous Compounds; D000516:alpha-Amylases