title: Parkin-mediated responses against infection and wound involve TSPO-VDAC complex in Drosophila.
abstract: Parkin, an E3 ubuquitin ligase associated with Parkinson's disease (PD), has recently been implicated in mediating innate immunity. However, molecular details regarding parkin-mediated immune response remain to be elucidated. Here, we identified mitochondrial TSPO-VDAC complex to genetically interact with parkin in mediating responses against infection and wound in Drosophila. The loss-of-function mutation in parkin results in defective immune response against bacterial infection. Additionally, parkin mutant larvae showed hypersensitivity against wound regardless of bacterial infection. Interestingly, the combinatorial trans-heterozygotic mutations in parkin and TSPO, or parkin and VDAC showed similar lethal tendency with parkin homozygous mutants. Furthermore, knockdown of TSPO alone also resulted in defective responses to infection and wound analogously to parkin mutants. Taken together, we propose that parkin cooperates with TSPO-VDAC complex to mediate responses against infection and wound.
authors.affiliation: Department of Brain & Cognitive Sciences, DGIST, Daegu 711-873, Republic of Korea.
authors.forename: Jae Ho
authors.initials: JH
authors.lastname: Cho
authors.affiliation: Department of Brain & Cognitive Sciences, DGIST, Daegu 711-873, Republic of Korea.
authors.forename: Jeong Hyang
authors.initials: JH
authors.lastname: Park
authors.affiliation: Department of Brain & Cognitive Sciences, DGIST, Daegu 711-873, Republic of Korea.
authors.forename: Chang Geon
authors.initials: CG
authors.lastname: Chung
authors.affiliation: Department of Brain & Cognitive Sciences, DGIST, Daegu 711-873, Republic of Korea.
authors.forename: Hyun-Jung
authors.initials: HJ
authors.lastname: Shim
authors.affiliation: Department of Brain & Cognitive Sciences, DGIST, Daegu 711-873, Republic of Korea.
authors.forename: Keun Hye
authors.initials: KH
authors.lastname: Jeon
authors.affiliation: Department of Brain & Cognitive Sciences, DGIST, Daegu 711-873, Republic of Korea.
authors.forename: Seong-Woon
authors.initials: SW
authors.lastname: Yu
authors.affiliation: Department of Brain & Cognitive Sciences, DGIST, Daegu 711-873, Republic of Korea. Electronic address: sblee@dgist.ac.kr.
authors.forename: Sung Bae
authors.initials: SB
authors.lastname: Lee
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D029721:Drosophila Proteins / Q000235:genetics / Q000276:immunology*; D004331:Drosophila melanogaster / Q000235:genetics / Q000276:immunology*; D055785:Gene Knockdown Techniques; D017344:Genes, Insect; D007113:Immunity, Innate / Q000235:genetics; D007239:Infections / Q000235:genetics / Q000276:immunology; D024101:Mitochondrial Proteins / Q000235:genetics / Q000276:immunology; D009154:Mutation; D018079:Receptors, GABA / Q000276:immunology; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000276:immunology*; D050994:Voltage-Dependent Anion Channels / Q000276:immunology; D014947:Wounds and Injuries / Q000235:genetics / Q000276:immunology
chemical_list: D029721:Drosophila Proteins; D024101:Mitochondrial Proteins; D018079:Receptors, GABA; D050994:Voltage-Dependent Anion Channels; C494761:porin protein, Drosophila; D044767:Ubiquitin-Protein Ligases; C487430:park protein, Drosophila
keywords: Innate immune response; Parkin; Septic injury; TSPO; VDAC (porin); Wound