title: Pyrethroid resistance in Culex pipiens mosquitoes.
abstract: Mosquitoes within the Culex pipiens complex are widely distributed and important in the transmission of many human diseases. Insecticides, pyrethroids in particular, remain a mainstay for control of these important vectors. In this paper we review what is known about the levels, mechanisms and fitness costs of pyrethroid resistance in Cx. pipiens. Pyrethroid resistance in Cx. pipiens is a global problem, and resistance ratios of up to 7000-fold have been found in larvae of field collected mosquitoes. However, there is considerable variation between populations, indicating significant geographic heterogeneity of the resistance. The two major mechanisms of resistance to pyrethroids in Culex are mutations in Vssc (target site insensitivity) and overexpression of cytochrome P450(s) (increased detoxification). The most frequently reported Vssc mutation is L1014F (i.e. kdr), which has been found throughout the world. The L1014S mutation has been found in Cx. p. pallens from Japan and China, and in Cx. p. pipiens from China. The L1014C mutation has only been reported for Cx. p. pipens molestus from China and the V1016G mutation has only been reported from Saudi Arabia. Studies on the P450s of Cx. pipiens have identified several that are overexpressed (measured as transcript levels) in pyrethroid resistant strains. CYP9M10 is consistently overexpressed in pyrethroid resistant Cx. pipiens from at least seven countries, suggesting this P450 might be of global importance in resistance. Both CYP9M10-mediated pyrethroid resistance and kdr have fitness costs in the absence of insecticides under certain environmental conditions. Research needs and future directions are discussed.
authors.affiliation: Department of Entomology, Comstock Hall, Cornell University, Ithaca, New York, USA. Electronic address: JGS5@cornell.edu.
authors.forename: Jeffrey G
authors.initials: JG
authors.lastname: Scott
authors.affiliation: Department of Entomology, Comstock Hall, Cornell University, Ithaca, New York, USA; California Department of Public Health, Vector-Borne Disease Section, 850 Marina Bay Pkwy, Richmond, California, USA.
authors.forename: Melissa Hardstone
authors.initials: MH
authors.lastname: Yoshimizu
authors.affiliation: Department of Entomology, Comstock Hall, Cornell University, Ithaca, New York, USA; Department of Medical Entomology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjukuku, Tokyo, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Kasai
mesh_terms: D000818:Animals; D003465:Culex / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D006801:Humans; D007303:Insect Vectors; D007305:Insecticide Resistance / Q000235:genetics; D007306:Insecticides / Q000633:toxicity*; D011722:Pyrethrins / Q000633:toxicity*
chemical_list: D007306:Insecticides; D011722:Pyrethrins
keywords: Cytochrome P450 monooxygenase; Fitness costs; Knockdown resistance; Mechanisms of resistance; Pyrethroid insecticides; kdr