title: Feasible quantitative detection of cytomegalovirus from urine sediment in stem cell transplant patients.
abstract: BACKGROUND
  Urine is an important source for the detection of infections caused by CMV in stem cell transplant patients. Currently, there is no agreement about the type of urine specimen. In order to investigate which is the better specimen type for quantitative detection of CMV, we compared the results from urine supernatant and sediment from the same patients.
  
  
  METHODS
  Seventy urine specimens were collected from patients with hematological disorders or solid tumors. After performing shell vial culture, residual urine specimens were centrifuged. Then, 10 mL of each urine supernatant and sediment were taken and immediately frozen at -70 degrees C. Afterwards, archived urine specimens were thawed at room temperature and CMV-quantitative PCR was performed on both the supernatant and sediment fraction of urine. The results from each patient were reviewed for CMV antigenemia, blood shell vial culture, CMV-IgM or IgG, and clinical symptoms.
  
  
  RESULTS
  CMV-qPCR results for the urine sediment fraction revealed a significant difference (p = 0.012) between the active CMV infection group and the latent CMV infection group. In addition, receiver operating characteristic curves for active CMV infection revealed that CMV-qPCR using urine sediment produced more accurate results than urine supernatant.
  
  
  CONCLUSIONS
  These findings suggest that the sediment fraction of urine is a more suitable specimen in CMV-qPCR testing.
authors.forename: Min-Gu
authors.initials: MG
authors.lastname: Kang
authors.forename: Min-Joong
authors.initials: MJ
authors.lastname: Jang
authors.forename: Seung-Yeob
authors.initials: SY
authors.lastname: Lee
authors.forename: Hye-Ran
authors.initials: HR
authors.lastname: Kim
authors.forename: Seok-Yong
authors.initials: SY
authors.lastname: Choi
authors.forename: Jong-Hee
authors.initials: JH
authors.lastname: Shin
authors.forename: Soon-Pal
authors.initials: SP
authors.lastname: Suh
authors.forename: Dong-Wook
authors.initials: DW
authors.lastname: Ryang
authors.forename: Myung-Geun
authors.initials: MG
authors.lastname: Shin
mesh_terms: D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000652:urine; D000956:Antigens, Viral / Q000652:urine; D002648:Child; D002675:Child, Preschool; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000652:urine*; D005260:Female; D019337:Hematologic Neoplasms / Q000628:therapy / Q000652:urine; D006801:Humans; D007074:Immunoglobulin G / Q000652:urine; D007075:Immunoglobulin M / Q000652:urine; D007223:Infant; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000628:therapy / Q000652:urine; D060888:Real-Time Polymerase Chain Reaction; D013048:Specimen Handling; D033581:Stem Cell Transplantation / Q000009:adverse effects*; D016482:Urinalysis / Q000379:methods*; D014766:Viremia / Q000652:urine*; D055815:Young Adult
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M