title: Short Communication: Contribution of Voltage-Gated Sodium Channels to the Rabbit Cone Electroretinograms.
abstract: Although the rabbit eye is of a similar size to the human eye, our limited understanding of the differences in retinal physiology to other species hinders its use in retinal research. The role of voltage-gated sodium channels (Nav) in the propagation of excitatory potentials along bipolar cells remains unclear, as conflicting data have been reported in the rabbit. The present study assesses the relative contributions of Nav to the scotopic and photopic flash ERGs as well as the wavelength-dependence of Nav blockade on the rabbit flicker ERG.
  
  
  
  Tetrodotoxin (TTX, 1 μM) was injected into the vitreous cavity of Chinchilla bastard rabbits. Scotopic ERGs were evoked by white flashes ranging from 10(-5) to 10 cds m(-2), photopic ERGs on a background of 25 cdm(-2) using flash intensities of 0.032-25 cds m(-2). Flicker ERGs (3-50 Hz) were elicited by blue, green and yellow stimuli at 2.34 cds m(-2) on a white background of 30 cdm(-2).
  
  
  
  The a- and b-waves of the scotopic ERG were unaffected by intravitreal injection of the Nav blocker TTX. In contrast, the b-wave, but not the a-wave, of the photopic ERG was selectively blocked by TTX. The reduction by TTX of the flicker ERG was greater for blue than for green and yellow stimuli.
  
  
  
  The data suggest that Nav selectively contribute to the generation of the photopic b-wave in the rabbit, indicating that they play an important role in the propagation of excitatory signals on bipolar cells in the cone, but not rod pathways. Importantly, the present study resolves conflicting previous reports into the role of Nav in the retinal function of the rabbit.
authors.affiliation: a Division of Ocular Biology and Therapeutics , UCL Institute of Ophthalmology , London , UK and.
authors.forename: Silke
authors.initials: S
authors.lastname: Becker
authors.affiliation: b NIHR Biomedical Research Centre for at Moorfields Eye Hospital and UCL Institute of Ophthalmology , London , UK.
authors.forename: Hari
authors.initials: H
authors.lastname: Jayaram
authors.affiliation: b NIHR Biomedical Research Centre for at Moorfields Eye Hospital and UCL Institute of Ophthalmology , London , UK.
authors.forename: Graham E
authors.initials: GE
authors.lastname: Holder
authors.affiliation: b NIHR Biomedical Research Centre for at Moorfields Eye Hospital and UCL Institute of Ophthalmology , London , UK.
authors.forename: G Astrid
authors.initials: GA
authors.lastname: Limb
mesh_terms: D000818:Animals; D004596:Electroretinography / Q000379:methods*; D005260:Female; D023421:Models, Animal; D010775:Photic Stimulation; D011817:Rabbits; D017949:Retinal Cone Photoreceptor Cells / Q000502:physiology*; D061566:Voltage-Gated Sodium Channels / Q000378:metabolism*
chemical_list: D061566:Voltage-Gated Sodium Channels
keywords: Electroretinogram; rabbit; retina; tetrodotoxin; voltage-gated sodium channels