title: Favourable effects of grape seed extract on intestinal epithelial differentiation and barrier function in IL10-deficient mice.
abstract: The impairment in the rate of cell proliferation and differentiation leads to a negative consequence on the renewal of the intestinal epithelium, which is the aetiological factor of a number of digestive diseases. Grape seed extract (GSE), a rich source of proanthocyanidins, is known for its beneficial health effects. The present study evaluated the beneficial effects of GSE on colonic cell differentiation and barrier function in IL10-deficient mice. Female mice aged 6 weeks were randomised into two groups and given drinking-water containing 0 or 0.1 % GSE (w/v) for 12 weeks. GSE supplementation decreased serum TNF-α level and intestinal permeability, and increased the colonic goblet cell density that was associated with increased mRNA expression of mucin (Muc)-2. Immunohistochemical analyses showed lower accumulation of β-catenin in the crypts of colon tissues of the GSE-supplemented mice, which was associated with a decreased mRNA expression of two downstream effectors of Wingless and Int (Wnt)/catenin signalling, myelocytomatosis oncogene protein (Myc) and cyclin D1 (Ccnd1). Consistently, GSE supplementation decreased the number of colonic proliferating cell nuclear antigen-positive cells, a well-known cell proliferation marker, and a weakened extracellular signal-regulated kinases 1 and 2 (ERK1/2) signalling. In summary, these data indicate that supplementation of 0.1 % GSE for 12 weeks improved gut barrier function and colonic cell differentiation in the IL10-deficient mice probably via inhibiting Wnt/β-catenin pathway.
authors.affiliation: School of Food Science, Washington State University,Pullman,WA99164,USA.
authors.forename: Guan
authors.initials: G
authors.lastname: Yang
authors.affiliation: School of Food Science, Washington State University,Pullman,WA99164,USA.
authors.forename: Yansong
authors.initials: Y
authors.lastname: Xue
authors.affiliation: School of Food Science, Washington State University,Pullman,WA99164,USA.
authors.forename: Hanying
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Animal Science,Washington State University,Pullman,WA99164,USA.
authors.forename: Min
authors.initials: M
authors.lastname: Du
authors.affiliation: School of Food Science, Washington State University,Pullman,WA99164,USA.
authors.forename: Mei-Jun
authors.initials: MJ
authors.lastname: Zhu
mesh_terms: D000818:Animals; D002452:Cell Count; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation; D003106:Colon / Q000737:chemistry / Q000166:cytology*; D004032:Diet; D060766:Drinking Water; D004847:Epithelial Cells / Q000737:chemistry / Q000166:cytology*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005260:Female; D020397:Goblet Cells / Q000166:cytology; D056604:Grape Seed Extract / Q000008:administration & dosage*; D007150:Immunohistochemistry; D016753:Interleukin-10 / Q000172:deficiency*; D007422:Intestines / Q000502:physiology*; D051379:Mice; D055262:Mucin-2 / Q000235:genetics; D044945:Proanthocyanidins; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D015398:Signal Transduction / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D051176:beta Catenin / Q000032:analysis
chemical_list: D060766:Drinking Water; D056604:Grape Seed Extract; C524626:Muc2 protein, mouse; D055262:Mucin-2; D044945:Proanthocyanidins; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D051176:beta Catenin; D016753:Interleukin-10; D048049:Extracellular Signal-Regulated MAP Kinases
keywords: Catenin; Grape seed extract; Gut epithelium barrier; IL10; Polyphenol