title: Transcutaneous Peptide Immunotherapy of Japanese Cedar Pollinosis Using Solid-in-Oil Nanodispersion Technology.
abstract: Peptide immunotherapy is an attractive approach to relieve allergic symptoms such as rhinitis and asthma. Treatment of Japanese cedar pollinosis (Cryptomeria japonica; Cj), from which over one quarter of Japanese population suffer, is becoming a great concern. Recently, oral feeding of a peptide (7crp) consisting of seven immunodominant human T cell epitopes derived from two enzymes present in Cj pollen was demonstrated to have a benefit in treating Cj pollinosis. In this work, we aimed to apply a novel transcutaneous administration system as a simple and easy peptide delivery for an immunotherapy using a T cell epitope peptide. A modified 7crp peptide (7crpR) which contained triarginine linkers between each epitopes was designed to increase water solubility and was encapsulated in a unique solid-in-oil (S/O) nanodispersion. The S/O nanodispersion consists of a nano-sized peptide-surfactant complex dispersed in an oil vehicle. The S/O nanopartilces having an average diameter of 230 nm facilitated the permeation of the peptide 7crpR into the skin and suppressed serum total IgE and antigen-specific IgE levels in a Cj pollinosis mouse model. Transcutaneous administration of the T cell epitope peptide using the S/O nanodispersion system has potential for future simple and easy immunotherapy of Cj pollinosis.
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Fukuoka, 819-0395, Japan.
authors.forename: Momoko
authors.initials: M
authors.lastname: Kitaoka
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Fukuoka, 819-0395, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Shin
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Fukuoka, 819-0395, Japan.|Center for Future Chemistry, Kyushu University, 744 Moto-oka, Fukuoka, 819-0395, Japan.
authors.forename: Noriho
authors.initials: N
authors.lastname: Kamiya
authors.affiliation: Department of Chemical Engineering, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Fukuoka, 819-0395, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Kawabe
authors.affiliation: Department of Chemical Engineering, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Fukuoka, 819-0395, Japan.
authors.forename: Masamichi
authors.initials: M
authors.lastname: Kamihira
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Fukuoka, 819-0395, Japan. m-goto@mail.cstm.kyushu-u.ac.jp.|Center for Future Chemistry, Kyushu University, 744 Moto-oka, Fukuoka, 819-0395, Japan. m-goto@mail.cstm.kyushu-u.ac.jp.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Goto
mesh_terms: D000279:Administration, Cutaneous; D000485:Allergens / Q000737:chemistry; D000595:Amino Acid Sequence; D000818:Animals; D028203:Cedrus / Q000737:chemistry*; D032485:Cryptomeria / Q000737:chemistry*; D018984:Epitopes, T-Lymphocyte / Q000008:administration & dosage / Q000737:chemistry; D005260:Female; D007167:Immunotherapy / Q000379:methods; D051379:Mice; D008969:Molecular Sequence Data; D009821:Oils / Q000737:chemistry*; D010455:Peptides / Q000008:administration & dosage / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry; D011058:Pollen / Q000737:chemistry; D006255:Rhinitis, Allergic, Seasonal / Q000188:drug therapy*; D013678:Technology, Pharmaceutical / Q000379:methods
chemical_list: D000485:Allergens; D018984:Epitopes, T-Lymphocyte; D009821:Oils; D010455:Peptides; D010940:Plant Proteins
keywords: epitope peptide; immunotherapy; pollinosis; solid-in-oil nanodispersion; transdermal drug delivery