title: An innovative method for immobilizing sucrose isomerase on ε-poly-L-lysine modified mesoporous TiO2.
abstract: Sucrose isomerase (SIase) is the key enzyme in the enzymatic synthesis of isomaltulose. Mesoporous titanium dioxide (M-TiO2) and ε-poly-L-lysine-functionalized M-TiO2 (EPL-M-TiO2) were prepared as carriers for immobilizing SIase. SIase was effectively immobilized on EPL-M-TiO2 (SI-EPL-M-TiO2) with an enzyme activity of 39.41 U/g, and the enzymatic activity recovery rate up to 93.26%. The optimal pH and temperature of immobilized SIase were 6.0 and 30° C, respectively. SI-EPL-M-TiO2 was more stable in pH and thermal tests than SIase immobilized on M-TiO2 and free SIase. K(m) of SI-EPL-M-TiO2 was 204.92 mmol/L, and vmax was 45.7 μmol/L/s. Batch catalysis reaction of sucrose by SI-EPL-M-TiO2 was performed under the optimal conditions. The half-life period of SI-EPL-M-TiO2 under continuous reaction was 114 h, and the conversion rate of sucrose after 16 batches consistently remained at around 95%, which indicates that SI-EPL-M-TiO2 has good operational stability. Thus, SI-EPL-M-TiO2 can be used as a biocatalyst in food industries.
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing Tech University, 30 Puzhu South Road, Nanjing 211816, China.
authors.forename: Lingtian
authors.initials: L
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing Tech University, 30 Puzhu South Road, Nanjing 211816, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing Tech University, 30 Puzhu South Road, Nanjing 211816, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Chi
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing Tech University, 30 Puzhu South Road, Nanjing 211816, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing Tech University, 30 Puzhu South Road, Nanjing 211816, China.
authors.forename: Xiaohai
authors.initials: X
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing Tech University, 30 Puzhu South Road, Nanjing 211816, China. Electronic address: lisha@njtech.edu.cn.
authors.forename: Sha
authors.initials: S
authors.lastname: Li
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing Tech University, 30 Puzhu South Road, Nanjing 211816, China. Electronic address: xuh@njtech.edu.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
mesh_terms: D001671:Biochemistry / Q000379:methods*; D004800:Enzymes, Immobilized / Q000737:chemistry; D005964:Glucosyltransferases / Q000737:chemistry*; D006207:Half-Life; D007536:Isomerism; D011107:Polylysine / Q000737:chemistry*; D016062:Porosity; D013696:Temperature
chemical_list: D004800:Enzymes, Immobilized; D011107:Polylysine; D005964:Glucosyltransferases; C116853:sucrose isomerase
keywords: Immobilization; Isomaltulose; Isomaltulose (PubChem CID: 439559); M-TiO(2); Sucrose (PubChem CID: 5988); Sucrose isomerase; Titanium dioxide (PubChem CID: 26042); Trehalulose (PubChem CID: 162104); ε-Poly-l-lysine