title: Trolox enhances follicular survival after ovarian tissue autograft in squirrel monkey (Saimiri collinsi).
abstract: The aim of this study was to evaluate ovarian tissue pre-treatment with 50 µM Trolox followed by heterotopic transplantation in squirrel monkeys (Saimiri collinsi) and to assess tissue functionality via immunohistochemical analysis of the stroma and ovarian follicles. Five healthy and sexually mature squirrel monkey (Saimiri collinsi) females were used. Heterotopic autografting of fresh ovarian tissue with or without previous exposure to the antioxidant Trolox was performed and grafts were recovered for analysis 7 days later. Tissue vascularisation was confirmed by both macroscopic inspection and cluster of differentiation 31 (CD31) staining. Trolox prevented massive follicular activation and kept the percentages of morphologically normal follicles higher than in untreated grafts. Expression of anti-Müllerian hormone in developing follicles was observed only in controls and Trolox-treated grafts. Also, immunostaining for growth differentiation factor-9 was positive only in primordial follicles from controls and from Trolox-treated grafts. Although Trolox improved follicular quality and avoided apoptosis in stromal cells, ovarian tissue fibrosis was increased in Trolox-treated grafts, mainly due to an increase in collagen Type I synthesis.
authors.affiliation: Laboratory of Wild Animal Biology and Medicine, Federal University of Pará, Rua Augusto Correa, Campus Básico, CEP 66075-110, Castanhal, Pará, Brazil.|National Primate Centre, Secretary of Health Policy, Ministry of Health, Conj. Julia Seffer, Rua Coletora Leste, S/N, Águas Lindas, CEP 67020-380, Ananindeua, Pará, Brazil.
authors.forename: Sarah R
authors.initials: SR
authors.lastname: Scalercio
authors.affiliation: Pôle de Recherche en Gynécologie, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Avenue Mounier 52 bte Bl.52.02, 1200, Brussels, Belgium.
authors.forename: Christiani A
authors.initials: CA
authors.lastname: Amorim
authors.affiliation: Laboratory of Wild Animal Biology and Medicine, Federal University of Pará, Rua Augusto Correa, Campus Básico, CEP 66075-110, Castanhal, Pará, Brazil.
authors.forename: Danielle C
authors.initials: DC
authors.lastname: Brito
authors.affiliation: Oxidative Stress Research Laboratory, Federal University of Pará, Av. Generalíssimo Deodoro, 92, CEP 66055-240. Belém, Pará, Brazil.
authors.forename: Sandro
authors.initials: S
authors.lastname: Percário
authors.affiliation: Faculty of Veterinary Medicine, Ullevalsvn 71, P.O. Box 8146, Dep. N-0033, Oslo, Norway.
authors.forename: Irma C
authors.initials: IC
authors.lastname: Oskam
authors.affiliation: Laboratory of Wild Animal Biology and Medicine, Federal University of Pará, Rua Augusto Correa, Campus Básico, CEP 66075-110, Castanhal, Pará, Brazil.
authors.forename: Sheyla F S
authors.initials: SFS
authors.lastname: Domingues
authors.affiliation: Laboratory of Wild Animal Biology and Medicine, Federal University of Pará, Rua Augusto Correa, Campus Básico, CEP 66075-110, Castanhal, Pará, Brazil.|Faculty of Veterinary Medicine, Utrecht University, Yalelaan 104, 3584CM, Utrecht, The Netherlands.|Corresponding author. Email: r.rodriguesdossantos@pq.cnpq.br.
authors.forename: Regiane R
authors.initials: RR
authors.lastname: Santos
keywords: apoptosis; fibrosis; grafting; pre-antral follicles; vascularisation