title: Production of gamma-aminobutyric acid from glucose by introduction of synthetic scaffolds between isocitrate dehydrogenase, glutamate synthase and glutamate decarboxylase in recombinant Escherichia coli.
abstract: Escherichia coli were engineered for the direct production of gamma-aminobutyric acid from glucose by introduction of synthetic protein scaffold. In this study, three enzymes consisting GABA pathway (isocitrate dehydrogenase, glutamate synthase and glutamate decarboxylase) were connected via synthetic protein scaffold. By introduction of scaffold, 0.92g/L of GABA was produced from 10g/L of glucose while no GABA was produced in wild type E. coli. The optimum pH and temperature for GABA production were 4.5 and 30°C, respectively. When competing metabolic network was inactivated by knockout mutation, maximum GABA concentration of 1.3g/L was obtained from 10g/L glucose. The recombinant E. coli strain which produces GABA directly from glucose was successfully constructed by introduction of protein scaffold.
authors.affiliation: Department of Chemical Engineering, University of Ulsan, 93 Daehakro, Nam-gu, Ulsan 680-749, Republic of Korea.
authors.forename: Van Dung
authors.initials: VD
authors.lastname: Pham
authors.affiliation: Department of Biotechnology&Bioengineering, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 500-757, Republic of Korea.
authors.forename: Seung Hwan
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Environmental Engineering and Energy, Myongji University, San 38-2, Nam-dong, Cheoin-gu, Gyeonggido, Yongin-si 449-728, Republic of Korea.
authors.forename: Si Jae
authors.initials: SJ
authors.lastname: Park
authors.affiliation: Department of Chemical Engineering, University of Ulsan, 93 Daehakro, Nam-gu, Ulsan 680-749, Republic of Korea. Electronic address: shhong@ulsan.ac.kr.
authors.forename: Soon Ho
authors.initials: SH
authors.lastname: Hong
mesh_terms: D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D005968:Glutamate Decarboxylase / Q000235:genetics / Q000378:metabolism; D005970:Glutamate Synthase / Q000235:genetics / Q000378:metabolism; D007521:Isocitrate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D015202:Protein Engineering / Q000379:methods*; D058615:Synthetic Biology; D005680:gamma-Aminobutyric Acid / Q000096:biosynthesis*
chemical_list: D005680:gamma-Aminobutyric Acid; D007521:Isocitrate Dehydrogenase; D005970:Glutamate Synthase; D005968:Glutamate Decarboxylase; D005947:Glucose
keywords: GABA, Escherichia coli, Synthetic scaffold protein, Synthetic biology