title: Intratesticular alpha1-adrenergic receptors mediate stress-disturbed transcription of steroidogenic stimulator NUR77 as well as steroidogenic repressors DAX1 and ARR19 in Leydig cells of adult rats.
abstract: The aim of the present study was to define the role of testicular α1-adrenergic receptors (α1-ADRs) in stress-triggered adaptation of testosterone-producing Leydig cells of adult rats. Results showed that in vivo blockade of testicular α1-ADRs prevented partial recovery of circulating androgen levels registered after 10× repeated immobilization stress (10 × IMO). Moreover, α1-ADR-blockade diminished 10 × IMO-triggered recovery of Leydig cell androgen production, and abolished mitochondrial membrane potential recovery. In the same cells, 10 × IMO-induced increase in Star transcript was abolished, Lhcgr transcript decreased, while transcription of other steroidogenic proteins was not changed. α1-ADR-blockade recovered stress-induced decrease of Nur77, one of the main steroidogenic stimulator, while significantly reduced 10 × IMO-increased in the transcription of the main steroidogenic repressors, Arr19 and Dax1. In vitro experiments revealed an adrenaline-induced α1-ADR-mediated decrease in Nur77 transcription in Leydig cells. Adrenaline-induced increase of repressor Dax1 also involves ADRs in Leydig cells. Accordingly, α1-ADRs participate in some of the stress-triggered effects on the steroidogenic machinery of Leydig cells.
authors.affiliation: Reproductive Endocrinology and Signaling Group, Faculty of Sciences, University of Novi Sad, Novi Sad, Serbia.
authors.forename: Natasa J
authors.initials: NJ
authors.lastname: Stojkov-Mimic
authors.affiliation: Reproductive Endocrinology and Signaling Group, Faculty of Sciences, University of Novi Sad, Novi Sad, Serbia.
authors.forename: Maja M
authors.initials: MM
authors.lastname: Bjelic
authors.affiliation: Reproductive Endocrinology and Signaling Group, Faculty of Sciences, University of Novi Sad, Novi Sad, Serbia.
authors.forename: Sava M
authors.initials: SM
authors.lastname: Radovic
authors.affiliation: Reproductive Endocrinology and Signaling Group, Faculty of Sciences, University of Novi Sad, Novi Sad, Serbia.
authors.forename: Aleksandar I
authors.initials: AI
authors.lastname: Mihajlovic
authors.affiliation: Reproductive Endocrinology and Signaling Group, Faculty of Sciences, University of Novi Sad, Novi Sad, Serbia.
authors.forename: Srdjan J
authors.initials: SJ
authors.lastname: Sokanovic
authors.affiliation: Reproductive Endocrinology and Signaling Group, Faculty of Sciences, University of Novi Sad, Novi Sad, Serbia.
authors.forename: Aleksandar Z
authors.initials: AZ
authors.lastname: Baburski
authors.affiliation: Reproductive Endocrinology and Signaling Group, Faculty of Sciences, University of Novi Sad, Novi Sad, Serbia.
authors.forename: Marija M
authors.initials: MM
authors.lastname: Janjic
authors.affiliation: Reproductive Endocrinology and Signaling Group, Faculty of Sciences, University of Novi Sad, Novi Sad, Serbia.
authors.forename: Tatjana S
authors.initials: TS
authors.lastname: Kostic
authors.affiliation: Reproductive Endocrinology and Signaling Group, Faculty of Sciences, University of Novi Sad, Novi Sad, Serbia. Electronic address: silvana.andric@dbe.uns.ac.rs.
authors.forename: Silvana A
authors.initials: SA
authors.lastname: Andric
mesh_terms: D000728:Androgens / Q000096:biosynthesis / Q000097:blood; D000818:Animals; D053898:Biosynthetic Pathways; D056970:Co-Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D057137:DAX-1 Orphan Nuclear Receptor / Q000235:genetics / Q000378:metabolism*; D007985:Leydig Cells / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D057105:Nuclear Receptor Subfamily 4, Group A, Member 1 / Q000235:genetics* / Q000378:metabolism; D017208:Rats, Wistar; D018340:Receptors, Adrenergic, alpha-1 / Q000502:physiology*; D013312:Stress, Physiological; D013739:Testosterone / Q000096:biosynthesis / Q000097:blood; D014158:Transcription, Genetic
chemical_list: C000607533:ARR19 protein, rat; D000728:Androgens; D056970:Co-Repressor Proteins; D057137:DAX-1 Orphan Nuclear Receptor; C539967:Nr0b1 protein, rat; C539820:Nr4a1 protein, rat; D057105:Nuclear Receptor Subfamily 4, Group A, Member 1; D018340:Receptors, Adrenergic, alpha-1; D013739:Testosterone; D007986:Luteinizing Hormone
keywords: Arr19; Dax1; Intratesticular α1-ADRs; Leydig cells; Nur77; Stress