title: DNA damage and oxidative status in PFAPA syndrome.
abstract: OBJECTIVE
  PFAPA syndrome is a clinical entity of unknown etiology which presents with periodic episodes of fever, aphthous stomatitis, tonsillitis or pharyngitis, and cervical adenitis. In this study we investigated DNA damage and the oxidative stress parameters in patients diagnosed with PFAPA, to elucidate the underlying pathophysiological mechanism of this syndrome.
  
  
  METHODS
  Thirty-one patients diagnosed with PFAPA (Group 1), 22 patients diagnosed with normal tonsillitis or pharyngitis (Group 2), and 20 healthy volunteers (Group 3) were included in our study. Heparinized peripheral blood samples were drawn from all patients and volunteers. DNA damage was assessed by single cell alkaline electrophoresis assay in peripheral mononuclear leukocytes. Plasma levels of total antioxidant status (TAS) and total oxidative status (TOS) were determined by using a novel automated measurement method, and oxidative stress index (OSI) was calculated.
  
  
  RESULTS
  DNA damage in the mononuclear leukocytes of Group 1 was significantly higher than that of Group 2 and Group 3. The oxidative stress parameters revealed that the TOS and OSI values of Group 1 were significantly higher than those of Group 2 and Group 3. TAS values of Group 1 were significantly lower than those of Group 2 and Group 3. Correlation analysis of Group 1 demonstrated a significant correlation between TOS, one of the oxidative stress parameters, and DNA damage. Correlations between DNA damage and C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR) values were also significant.
  
  
  CONCLUSIONS
  Our study indicated that both the inflammatory and the oxidative stress parameters were significantly increased in patients with PFAPA syndrome, accompanied by a significant positive correlation between DNA damage and oxidative stress.
authors.affiliation: Bezmialem Vakif University, Faculty of Medicine, Department of Otorhinolaryngology, Fatih, Istanbul, Turkey. Electronic address: selahattintugrul@yahoo.com.
authors.forename: Selahattin
authors.initials: S
authors.lastname: Tuğrul
authors.affiliation: Bayrampasa State Hospital, Department of Otorhinolaryngology, Bayrampasa, Istanbul, Turkey. Electronic address: dr.remzidogan@gmail.com.
authors.forename: Remzi
authors.initials: R
authors.lastname: Doğan
authors.affiliation: Bezmialem Vakif University, Faculty of Medicine, Department of Biochemistry, Fatih, Istanbul, Turkey. Electronic address: abdurrahimkocyigit@yahoo.com.
authors.forename: Abdurrahim
authors.initials: A
authors.lastname: Kocyigit
authors.affiliation: Bezmialem Vakif University, Faculty of Medicine, Department of Paediatrics, Fatih, Istanbul, Turkey. Electronic address: dr.emeltorun@gmail.com.
authors.forename: Emel
authors.initials: E
authors.lastname: Torun
authors.affiliation: Bezmialem Vakif University, Faculty of Medicine, Department of Otorhinolaryngology, Fatih, Istanbul, Turkey. Electronic address: erolsent@gmail.com.
authors.forename: Erol
authors.initials: E
authors.lastname: Senturk
authors.affiliation: Bezmialem Vakif University, Faculty of Medicine, Department of Otorhinolaryngology, Fatih, Istanbul, Turkey. Electronic address: orhanent@yahoo.com.
authors.forename: Orhan
authors.initials: O
authors.lastname: Ozturan
mesh_terms: D001799:Blood Sedimentation; D002097:C-Reactive Protein / Q000378:metabolism; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D004249:DNA Damage; D005260:Female; D005334:Fever / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008199:Lymphadenitis / Q000235:genetics / Q000378:metabolism*; D008297:Male; D009333:Neck; D018384:Oxidative Stress; D010507:Periodicity; D010612:Pharyngitis / Q000235:genetics / Q000378:metabolism*; D013281:Stomatitis, Aphthous / Q000235:genetics / Q000378:metabolism*; D013577:Syndrome; D014069:Tonsillitis / Q000235:genetics / Q000378:metabolism*
chemical_list: D002097:C-Reactive Protein
keywords: DNA damage; Oxidative status; PFAPA