title: A comparison of high-throughput techniques for assaying circadian rhythms in plants.
abstract: Over the last two decades, the development of high-throughput techniques has enabled us to probe the plant circadian clock, a key coordinator of vital biological processes, in ways previously impossible. With the circadian clock increasingly implicated in key fitness and signalling pathways, this has opened up new avenues for understanding plant development and signalling. Our tool-kit has been constantly improving through continual development and novel techniques that increase throughput, reduce costs and allow higher resolution on the cellular and subcellular levels. With circadian assays becoming more accessible and relevant than ever to researchers, in this paper we offer a review of the techniques currently available before considering the horizons in circadian investigation at ever higher throughputs and resolutions.
authors.affiliation: Institute of Integrative Biology, University of Liverpool, Crown Street, Liverpool, UK.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Tindall
authors.affiliation: Institute of Integrative Biology, University of Liverpool, Crown Street, Liverpool, UK.
authors.forename: Jade
authors.initials: J
authors.lastname: Waller
authors.affiliation: Institute of Integrative Biology, University of Liverpool, Crown Street, Liverpool, UK.
authors.forename: Mark
authors.initials: M
authors.lastname: Greenwood
authors.affiliation: Institute of Integrative Biology, University of Liverpool, Crown Street, Liverpool, UK.
authors.forename: Peter D
authors.initials: PD
authors.lastname: Gould
authors.affiliation: Institute of Integrative Biology, University of Liverpool, Crown Street, Liverpool, UK.
authors.forename: James
authors.initials: J
authors.lastname: Hartwell
authors.affiliation: Institute of Integrative Biology, University of Liverpool, Crown Street, Liverpool, UK.
authors.forename: Anthony
authors.initials: A
authors.lastname: Hall
keywords: Circadian clock; Delayed fluorescence; High-throughput assay; Infra-red gas exchange; Leaf movement; Luciferase