title: The cold response of CBF genes in barley is regulated by distinct signaling mechanisms.
abstract: Cold acclimation ability is crucial in the winter survival of cereals. In this process CBF transcription factors play key role, therefore understanding the regulation of these genes might provide useful knowledge for molecular breeding. In the present study the signal transduction pathways leading to the cold induction of different CBF genes were investigated in barley cv. Nure using pharmacological approach. Our results showed that the cold induced expression of CBF9 and CBF14 transcription factors is regulated by phospholipase C, phospholipase D pathways and calcium. On the contrary, these pathways have negative effect on the cold induction of CBF12 that is regulated by a different, as yet unidentified pathway. The diversity in the regulation of these transcription factors corresponds to their sequence based phylogenetic relationships suggesting that their evolutionary separation happened on structural, functional and regulational levels as well. On the CBF effector gene level, the signaling regulation is more complex, resultant effect of multiple pathways.
authors.affiliation: Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Department of Plant Molecular Biology, H-2462 Martonvásár, Brunszvik u. 2, Hungary. Electronic address: toth.zsuzsa@agrar.mta.hu.
authors.forename: Zsuzsa
authors.initials: Z
authors.lastname: Marozsán-Tóth
authors.affiliation: Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Department of Plant Molecular Biology, H-2462 Martonvásár, Brunszvik u. 2, Hungary. Electronic address: vashegyi.ildiko@agrar.mta.hu.
authors.forename: Ildikó
authors.initials: I
authors.lastname: Vashegyi
authors.affiliation: Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Department of Plant Molecular Biology, H-2462 Martonvásár, Brunszvik u. 2, Hungary. Electronic address: galiba.gabor@agrar.mta.hu.
authors.forename: Gábor
authors.initials: G
authors.lastname: Galiba
authors.affiliation: Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Department of Plant Molecular Biology, H-2462 Martonvásár, Brunszvik u. 2, Hungary. Electronic address: toth.balazs@agrar.mta.hu.
authors.forename: Balázs
authors.initials: B
authors.lastname: Tóth
mesh_terms: D002118:Calcium / Q000378:metabolism; D003080:Cold Temperature; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D001467:Hordeum / Q000235:genetics* / Q000502:physiology*; D010739:Phospholipase D / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics*; D010738:Type C Phospholipases / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D010738:Type C Phospholipases; D010739:Phospholipase D; D002118:Calcium
keywords: Barley; Cold acclimation; Cold signaling; Freezing tolerance