title: Automated quantitative cytological analysis using portable microfluidic microscopy.
abstract: In this article, a portable microfluidic microscopy based approach for automated cytological investigations is presented. Inexpensive optical and electronic components have been used to construct a simple microfluidic microscopy system. In contrast to the conventional slide-based methods, the presented method employs microfluidics to enable automated sample handling and image acquisition. The approach involves the use of simple in-suspension staining and automated image acquisition to enable quantitative cytological analysis of samples. The applicability of the presented approach to research in cellular biology is shown by performing an automated cell viability assessment on a given population of yeast cells. Further, the relevance of the presented approach to clinical diagnosis and prognosis has been demonstrated by performing detection and differential assessment of malaria infection in a given sample.
authors.affiliation: Optics & Microfluidics Instrumentation Lab, Department of Instrumentation and Applied Physics, Indian Institute of Science, Bangalore, 560012, India.
authors.forename: Veerendra Kalyan
authors.initials: VK
authors.lastname: Jagannadh
authors.affiliation: Optics & Microfluidics Instrumentation Lab, Department of Instrumentation and Applied Physics, Indian Institute of Science, Bangalore, 560012, India.
authors.forename: Rashmi Sreeramachandra
authors.initials: RS
authors.lastname: Murthy
authors.affiliation: Optics & Microfluidics Instrumentation Lab, Department of Instrumentation and Applied Physics, Indian Institute of Science, Bangalore, 560012, India.
authors.forename: Rajesh
authors.initials: R
authors.lastname: Srinivasan
authors.affiliation: Optics & Microfluidics Instrumentation Lab, Department of Instrumentation and Applied Physics, Indian Institute of Science, Bangalore, 560012, India. saisiva.gorthi@iap.iisc.ernet.in.
authors.forename: Sai Siva
authors.initials: SS
authors.lastname: Gorthi
mesh_terms: D003584:Cytological Techniques; D004912:Erythrocytes / Q000469:parasitology; D006801:Humans; D008288:Malaria / Q000175:diagnosis; D050296:Microbial Viability; D044085:Microfluidics / Q000379:methods*; D008853:Microscopy; D012441:Saccharomyces cerevisiae / Q000166:cytology; D013194:Staining and Labeling
keywords: cell survival; cytodiagnosis; diagnostic imaging; microfluidic microscopy; microfluidics; point of care systems