title: A pospiviroid from symptomless portulaca plants closely related to iresine viroid 1.
abstract: In symptomless plants of portulaca a potential new pospiviroid was characterized. Analysis by both double and return PAGE showed the presence of a circular RNA. RT-PCR and sequencing revealed a genome of 351 nt with properties characteristic of members of the genus Pospiviroid and with highest sequence identity (circa 80%) with iresine viroid 1 (IrVd-1). The circular RNA from portulaca was shown to replicate independently in its original host, thus showing that it is indeed a viroid. Based on its molecular characteristics, it should be considered a new species. However, since no biological differences have yet been found with its closest relative IrVd-1, the viroid from portulaca does not fulfil all criteria for species demarcation of the ICTV.
authors.affiliation: National Plant Protection Organization, National Reference Centre, P.O. Box 9102, 6700 HC Wageningen, The Netherlands. Electronic address: j.t.j.verhoeven@nvwa.nl.
authors.forename: J Th J
authors.initials: JT
authors.lastname: Verhoeven
authors.affiliation: National Plant Protection Organization, National Reference Centre, P.O. Box 9102, 6700 HC Wageningen, The Netherlands.
authors.forename: J W
authors.initials: JW
authors.lastname: Roenhorst
authors.affiliation: Naktuinbouw, P.O. Box 40, 2370 AA Roelofarendsveen, The Netherlands.
authors.forename: M
authors.initials: M
authors.lastname: Hooftman
authors.affiliation: Naktuinbouw, P.O. Box 40, 2370 AA Roelofarendsveen, The Netherlands.
authors.forename: E T M
authors.initials: ET
authors.lastname: Meekes
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas (UPV-CSIC), Universidad Politécnica de Valencia, Valencia 46022, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Flores
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas (UPV-CSIC), Universidad Politécnica de Valencia, Valencia 46022, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Serra
mesh_terms: D001483:Base Sequence; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D031822:Portulaca / Q000821:virology*; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D014772:Viroids / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D017735:Virus Latency
chemical_list: D012367:RNA, Viral
keywords: IrVd-1; Iresine viroid 1; PoLVd; Portulaca latent viroid; Pospiviroid