title: Complete mitochondrial genome of Lutzomyia (Nyssomyia) umbratilis (Diptera: Psychodidae), the main vector of Leishmania guyanensis.
abstract: The nearly complete mitochondrial genome of Lutzomyia umbratilis Ward & Fraiha, 1977 (Psychodidae: Phlebotominae), considered as the main vector of Leishmania guyanensis, is presented. The sequencing has been performed on an Illumina Hiseq 2500 platform, with a genome skimming strategy. The full nuclear ribosomal RNA segment was also assembled. The mitogenome of L. umbratilis was determined to be at least 15,717 bp-long and presents an architecture found in many mitogenomes of insect (13 protein-coding genes, 22 transfer RNAs, two ribosomal RNAs, and one non-coding region also referred as the control region). The control region contains a large repeated element of c. 370 bp and a poly-AT region of unknown length. This is the first mitogenome of Psychodidae to be described.
authors.affiliation: a CNRS, Université Toulouse III Paul Sabatier, ENFA; UMR5174 EDB (Laboratoire Evolution et Diversité Biologique) , Toulouse , France.
authors.forename: Arthur
authors.initials: A
authors.lastname: Kocher
authors.affiliation: b Laboratoire des Identifications Fongiques et Entomo-parasitologiques , Mennecy , France.
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Gantier
authors.affiliation: a CNRS, Université Toulouse III Paul Sabatier, ENFA; UMR5174 EDB (Laboratoire Evolution et Diversité Biologique) , Toulouse , France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Holota
authors.affiliation: c INRA, UAR 1209 Département de génétique animale , INRA Auzeville , Castanet-Tolosan , France.|d GeT-PlaGe, Genotoul, INRA Auzeville , Castanet-Tolosan , France.
authors.forename: Céline
authors.initials: C
authors.lastname: Jeziorski
authors.affiliation: e Laboratoire d'Ecologie Alpine , CNRS UMR 5553, Université Joseph Fourier , Grenoble , France.
authors.forename: Eric
authors.initials: E
authors.lastname: Coissac
authors.affiliation: f UMR MIVEGEC (IRD 224 - CNRS 5290), Université de Montpellier) , Montpellier , France , and.
authors.forename: Anne-Laure
authors.initials: AL
authors.lastname: Bañuls
authors.affiliation: g Medical Entomology Unit , Institut Pasteur de la Guyane , Cayenne Cedex , French Guiana.
authors.forename: Romain
authors.initials: R
authors.lastname: Girod
authors.affiliation: g Medical Entomology Unit , Institut Pasteur de la Guyane , Cayenne Cedex , French Guiana.
authors.forename: Pascal
authors.initials: P
authors.lastname: Gaborit
authors.affiliation: a CNRS, Université Toulouse III Paul Sabatier, ENFA; UMR5174 EDB (Laboratoire Evolution et Diversité Biologique) , Toulouse , France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Murienne
mesh_terms: D000818:Animals; D054629:Genome, Mitochondrial; D019476:Insect Proteins / Q000235:genetics; D007303:Insect Vectors / Q000235:genetics* / Q000469:parasitology; D018334:Leishmania guyanensis; D024101:Mitochondrial Proteins / Q000235:genetics; D011576:Psychodidae / Q000235:genetics* / Q000469:parasitology; D012313:RNA / Q000235:genetics; D000077278:RNA, Mitochondrial; D012335:RNA, Ribosomal / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics
chemical_list: D019476:Insect Proteins; D024101:Mitochondrial Proteins; D000077278:RNA, Mitochondrial; D012335:RNA, Ribosomal; D012313:RNA; D012343:RNA, Transfer
keywords: Genome skimming; Sandfly; illumina Hiseq; leishmaniasis; next-generation sequencing; shotgun sequencing