title: Change of MicroRNA-134, CREB and p-CREB expression in epileptic rat.
abstract: OBJECTIVE
  To To investigate the changes of MicroRNA-134, CREB and p-CREB expression in epileptic rat brains in order to elucidate the molecular mechanisms of epilepsy, providing new ideas for clinical treatment.
  
  
  METHODS
  Sixty-four Spraque-Dawley (SD) rats were divided into groups randomly, including control group, six hours after seizure group, 24-hour group, three-day group, one-week group, two-week group, four-week group, and eight-week group. All groups were placed under a pilocarpine-induced epilepsy model except the control group, and all rats were decapitated in different points of time. Brain specimens were taken for quantitative PCR experiments, immunohistochemistry and Western blot experiments. The results of the epilepsy model groups and the control group were compared.
  
  
  RESULTS
  There were no significant differences between the six hours after seizure group, the 24-hour group and the control group about the MicroRNA-134 levels. MicroRNA-134 in the hippocampus tissue of the three-day group significantly reduced compared with the control group; same result was observed with the one-week, two-week, four-week and eight-week groups. The CREB and p-CREB levels in the three-day group's rat hippocampus significantly increased compared with the control group; and the high levels of CREB and p-CREB were constantly maintained in the one-week, two-week, four-week and eight-week groups.
  
  
  CONCLUSIONS
  The MicroRNA-134 level of the epileptic rat hippocampus is significantly lower than normal after three days, and continues to maintain a low level; while CREB and p-CREB levels are rsignificantly increased after three days, and continue to remain at a high level. MicroRNA-134 plays a role in inhibiting synaptic plasticity by inhibiting CREB and p-CREB expressions.
authors.affiliation: Department of ICU, Zaozhuang Municipal Hospital (Jining medical college affiliated hospital), Zaozhuang, Shandong, P. R. China. Electronic address: zhuyan3486@126.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of ICU, Zaozhuang Municipal Hospital (Jining medical college affiliated hospital), Zaozhuang, Shandong, P. R. China.
authors.forename: Cheng-Shan
authors.initials: CS
authors.lastname: Li
authors.affiliation: Department of ICU, Zaozhuang Municipal Hospital (Jining medical college affiliated hospital), Zaozhuang, Shandong, P. R. China.
authors.forename: Yuan-Ye
authors.initials: YY
authors.lastname: Wang
authors.affiliation: Department of Neurology, Qilu Hospital of Shandong University and Brain Science Research Institute, Shandong University, Jinan, Shandong, P. R. China.
authors.forename: Sheng-Nian
authors.initials: SN
authors.lastname: Zhou
keywords: CREB; Immunohistochemistry; MicroRNA-134; Quantitative PCR; Western blot; p-CREB