title: A microfabrication-free nanoliter droplet array for nucleic acid detection combined with isothermal amplification.
abstract: A nanoliter droplet array based on a hydrophilic-hydrophobic patterned chip is developed without using microfabrication technology. Combined with the isothermal amplification technology, it has been applied to perform nucleic acid detection, showing excellent specificity and sensitivity. As a versatile platform, it is used to detect three gene targets successfully.
authors.affiliation: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, P. R. China. zhlu@seu.edu.cn.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Ma
authors.forename: Weiwei
authors.initials: W
authors.lastname: Xu
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
authors.forename: Zuhong
authors.initials: Z
authors.lastname: Lu
authors.forename: Jiong
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D002417:Cattle; D055616:Microtechnology / Q000379:methods*; D036103:Nanotechnology / Q000379:methods; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D009696:Nucleic Acids / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000032:analysis
chemical_list: D009696:Nucleic Acids; D012710:Serum Albumin, Bovine