title: Biological diversity of cytochrome P450 redox partner systems.
abstract: Cytochrome P450 enzymes (P450s or CYPs) catalyze an enormous variety of oxidative reactions in organisms from all major domains of life. Their monooxygenase activity relies on the reductive scission of molecular oxygen (O2) bound to P450 heme iron, and thus on the delivery of two electrons to the heme iron at discrete points in the catalytic cycle. Early studies suggested that P450 redox partner machinery fell into only two major classes: either the eukaryotic diflavin enzyme NADPH-cytochrome P450 oxidoreductase, or bacterial/mitochondrial NAD(P)H-ferredoxin reductase and ferredoxin partners. However, more recent studies, aided by genome sequence data, reveal a much more complex scenario. Several new types of P450 redox partner systems have now been characterized, including P450s naturally linked to their redox partners, or to a component protein of their P450 electron delivery system. Other P450s have evolved to bypass requirements for redox partners, and instead react directly with hydrogen peroxide or NAD(P)H to facilitate oxidative or reductive catalysis. Further P450s are fused to non-redox partner enzymes and can catalyse consecutive reactions in a common pathway. This chapter describes the biochemistry and the enormous natural diversity of P450 redox systems, including descriptions of novel P450s fused to non-redox partner proteins.
authors.affiliation: Manchester Institute of Biotechnology, The University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK.
authors.forename: Kirsty J
authors.initials: KJ
authors.lastname: McLean
authors.forename: Dominika
authors.initials: D
authors.lastname: Luciakova
authors.forename: James
authors.initials: J
authors.lastname: Belcher
authors.forename: Kang Lan
authors.initials: KL
authors.lastname: Tee
authors.forename: Andrew W
authors.initials: AW
authors.lastname: Munro
mesh_terms: D000818:Animals; D001419:Bacteria / Q000201:enzymology / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006801:Humans; D024101:Mitochondrial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D009249:NADP / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010084:Oxidation-Reduction
chemical_list: D001426:Bacterial Proteins; D024101:Mitochondrial Proteins; D009249:NADP; D003577:Cytochrome P-450 Enzyme System