title: Low affinity binding of plasma proteins to lipid-coated quantum dots as observed by in situ fluorescence correlation spectroscopy.
abstract: Protein binding to lipid-coated nanoparticles has been pursued quantitatively by using fluorescence correlation spectroscopy. The binding of three important plasma proteins to lipid-enwrapped quantum dots (QDs) shows very low affinity, with an apparent dissociation coefficient in the range of several hundred micromolar. Thus, the tendency to adsorb is orders of magnitude weaker than for QDs coated with dihydrolipoic acid.
authors.affiliation: Institute of Applied Physics and Center for Functional Nanostructures, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany. uli@illinois.edu.
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Klapper
authors.forename: Pauline
authors.initials: P
authors.lastname: Maffre
authors.forename: Li
authors.initials: L
authors.lastname: Shang
authors.forename: Kristina N
authors.initials: KN
authors.lastname: Ekdahl
authors.forename: Bo
authors.initials: B
authors.lastname: Nilsson
authors.forename: Simon
authors.initials: S
authors.lastname: Hettler
authors.forename: Manuel
authors.initials: M
authors.lastname: Dries
authors.forename: Dagmar
authors.initials: D
authors.lastname: Gerthsen
authors.forename: G Ulrich
authors.initials: GU
authors.lastname: Nienhaus
mesh_terms: D001798:Blood Proteins / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D057446:Hydrodynamics; D008244:Lysophosphatidylcholines / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D011485:Protein Binding; D045663:Quantum Dots / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D001798:Blood Proteins; D008244:Lysophosphatidylcholines; C035255:myristoyllysophosphatidylcholine