title: Silencing SOX2 Expression by RNA Interference Inhibits Proliferation, Invasion and Metastasis, and Induces Apoptosis through MAP4K4/JNK Signaling Pathway in Human Laryngeal Cancer TU212 Cells.
abstract: SRY (sex determining region Y)-box 2 (SOX2) plays an important role in tumor cell metastasis and apoptosis. Laryngeal squamous cell carcinoma (LSCC), responsible for 1.5% of all cancers, is one of the most common head and neck malignancies. Accumulating evidence shows that SOX2 is overexpressed in several human tumors, including lung cancer, esophageal carcinoma, pancreatic carcinoma, breast cancer, ovarian carcinoma and glioma. Our study aimed to investigate the silencing effects of SOX2 expression using RNA interference (RNAi) on various biological processes in laryngeal cancer TU212 cells, including proliferation, apoptosis, invasion and metastasis. We also studied the involvement of the MAPK/JNK signaling pathway in the biological effects of SOX2 siRNA in TU212 cells. We found that silencing SOX2 decreased the proliferation, migration, and invasion of TU212 cells, and induced apoptosis. This effect of silencing SOX2 could be reversed by silencing MAP4K4. Therefore, we consider SOX2 as a key regulator of the upstream MAP4K4/JNK signaling pathways that could be a potential therapeutic target in the treatment of patients with or prevention of laryngeal cancer.
authors.affiliation: Department of Otorhinolaryngology, The First Affiliated Hospital of China Medical University, Shenyang, People’s
  Republic of China (NY, YW, LH, XL, XJ)
authors.forename: Ning
authors.initials: N
authors.lastname: Yang
authors.affiliation: Department of Otorhinolaryngology, The First Affiliated Hospital of China Medical University, Shenyang, People’s
  Republic of China (NY, YW, LH, XL, XJ)
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Otorhinolaryngology, The First Affiliated Hospital of China Medical University, Shenyang, People’s
  Republic of China (NY, YW, LH, XL, XJ)
authors.forename: Lian
authors.initials: L
authors.lastname: Hui
authors.affiliation: Department of Otorhinolaryngology, The First Affiliated Hospital of China Medical University, Shenyang, People’s
  Republic of China (NY, YW, LH, XL, XJ)
authors.forename: Xiaotian
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Otorhinolaryngology, The First Affiliated Hospital of China Medical University, Shenyang, People’s
  Republic of China (NY, YW, LH, XL, XJ)
authors.forename: Xuejun
authors.initials: X
authors.lastname: Jiang
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D002999:Clone Cells / Q000473:pathology; D018450:Disease Progression; D015536:Down-Regulation / Q000235:genetics; D004789:Enzyme Activation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism*; D007822:Laryngeal Neoplasms / Q000235:genetics / Q000473:pathology*; D020935:MAP Kinase Signaling System / Q000235:genetics*; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D010766:Phosphorylation / Q000235:genetics; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D055748:SOXB1 Transcription Factors / Q000172:deficiency / Q000235:genetics*
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D034741:RNA, Small Interfering; D055748:SOXB1 Transcription Factors; C117090:MAP4K4 protein, human; D017346:Protein Serine-Threonine Kinases; D048031:JNK Mitogen-Activated Protein Kinases
keywords: MAP4K4/JNK signaling pathways; SRY (sex determining region Y)-box 2 (SOX2); apoptosis; invasion; laryngeal cancer TU212 cells; metastasis; proliferation; siRNA