title: Functional behavior and gene expression of magnetic nanoparticle-loaded primary endothelial cells for targeting vascular stents.
abstract: OBJECTIVE
  To assess functional competence and gene expression of magnetic nanoparticle (MNP)-loaded primary endothelial cells (ECs) as potential cell-based therapy vectors.
  
  
  METHODS
  A quantitative tube formation, nitric oxide and adhesion assays were conducted to assess functional potency of the MNP-loaded ECs. A quantitative real-time PCR was used to profile genes in both MNP-loaded at static conditions and in vitro targeted ECs.
  
  
  RESULTS
  Functional behavior of MNP-loaded and unloaded cells was comparable. MNPs induce expression of genes involved in EC growth and survival, while repress genes involved in coagulation.
  
  
  CONCLUSIONS
  MNPs do not adversely affect cellular function. Gene expression indicates that targeting MNP-loaded ECs to vascular stents may potentially stimulate re-endothelialization of an implant and attenuate neointimal hyperplasia.
authors.affiliation: 1Department of Surgery, Drexel University College of Medicine, 245 North 15th Street, NCB Suite 7150, Mail Stop 413, Philadelphia, PA 19102, USA.
authors.forename: Fatema Tuj
authors.initials: FT
authors.lastname: Zohra
authors.affiliation: 1Department of Surgery, Drexel University College of Medicine, 245 North 15th Street, NCB Suite 7150, Mail Stop 413, Philadelphia, PA 19102, USA.
authors.forename: Mikhail
authors.initials: M
authors.lastname: Medved
authors.affiliation: 1Department of Surgery, Drexel University College of Medicine, 245 North 15th Street, NCB Suite 7150, Mail Stop 413, Philadelphia, PA 19102, USA.
authors.forename: Nina
authors.initials: N
authors.lastname: Lazareva
authors.affiliation: 1Department of Surgery, Drexel University College of Medicine, 245 North 15th Street, NCB Suite 7150, Mail Stop 413, Philadelphia, PA 19102, USA.
authors.forename: Boris
authors.initials: B
authors.lastname: Polyak
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D020869:Gene Expression Profiling; D008280:Magnetics; D053758:Nanoparticles; D051381:Rats; D015607:Stents
keywords: endothelial integrity and functional potency; gene expression; in-stent restenosis; magnetic cell delivery; magnetic nanoparticles; primary endothelial cells; re-endothelialization