title: Inhibitory activities of biflavonoids against amyloid-β peptide 42 cytotoxicity in PC-12 cells.
abstract: A major hallmark of Alzheimer's disease is the cerebral accumulation and resulting cytotoxicity of amyloid-β peptides, particularly Aβ42. In this study, we used an MTT assay to investigate the inhibitory activity of biflavonoids 1-22 against Aβ42 cytotoxicity in PC-12 cell cultures. Cytoprotective effects were observed for the following amentoflavone type biflavonoids: podocarpusflavone B 8, isoginkgetin 10, sciadopitysin 13, and kayaflavone 15. These biflavonoids exhibited strong activity in tested compounds, with EC50 values of 5.18, 10.77, 9.84, and 5.29 μM, respectively. Cell viability tests of PC-12 cells revealed that biflavonoids 13 and 15 had stronger inhibitory activities than apigenin 23 and (-)-epigallocatechin gallate 24.
authors.affiliation: School of Pharmacy, Shujitsu University, Nishigawara 1-6-1, Naka-ku, Okayama-shi, Okayama 703-8516, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Sasaki
authors.affiliation: Department of Pharmacognosy and Phytochemistry, Meiji Pharmaceutical University, Noshio 2-522-1, Kiyose-shi, Tokyo 204-8588, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Kitoh
authors.affiliation: Department of Pharmacognosy and Phytochemistry, Meiji Pharmaceutical University, Noshio 2-522-1, Kiyose-shi, Tokyo 204-8588, Japan.
authors.forename: Mariko
authors.initials: M
authors.lastname: Tsukada
authors.affiliation: Department of Pharmacognosy and Phytochemistry, Meiji Pharmaceutical University, Noshio 2-522-1, Kiyose-shi, Tokyo 204-8588, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Miki
authors.affiliation: Department of Pharmacognosy and Phytochemistry, Meiji Pharmaceutical University, Noshio 2-522-1, Kiyose-shi, Tokyo 204-8588, Japan.
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Koyama
authors.affiliation: Natural Products Chemistry Research Group, Program Study of Chemistry, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Jalan Ganesha 10, Bandung 40132, Indonesia.
authors.forename: Lia D
authors.initials: LD
authors.lastname: Juliawaty
authors.affiliation: Natural Products Chemistry Research Group, Program Study of Chemistry, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Jalan Ganesha 10, Bandung 40132, Indonesia.
authors.forename: Euis H
authors.initials: EH
authors.lastname: Hakim
authors.affiliation: Department of Pharmacognosy and Phytochemistry, Meiji Pharmaceutical University, Noshio 2-522-1, Kiyose-shi, Tokyo 204-8588, Japan.
authors.forename: Kunio
authors.initials: K
authors.lastname: Takahashi
authors.affiliation: Department of Pharmacognosy and Phytochemistry, Meiji Pharmaceutical University, Noshio 2-522-1, Kiyose-shi, Tokyo 204-8588, Japan. Electronic address: kaoru@my-pharm.ac.jp.
authors.forename: Kaoru
authors.initials: K
authors.lastname: Kinoshita
mesh_terms: D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000818:Animals; D044946:Biflavonoids / Q000737:chemistry* / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects*; D016716:PC12 Cells; D010446:Peptide Fragments / Q000633:toxicity*; D020011:Protective Agents / Q000737:chemistry* / Q000494:pharmacology*; D051381:Rats; D013329:Structure-Activity Relationship
chemical_list: D016229:Amyloid beta-Peptides; D044946:Biflavonoids; D010446:Peptide Fragments; D020011:Protective Agents; C075222:amyloid beta-protein (1-42)
keywords: Alzheimer’s disease; Amentoflavone; Amyloid-β; Biflavonoids; PC-12 cells