title: Selective Cell Adhesion and Biosensing Applications of Bio-Active Block Copolymers Prepared by CuAAC/Thiol-ene Double Click Reactions.
abstract: N-Acetyl-l-cysteine (NAC)-capped poly(methyl methacrylate)-b-polycaprolactone block copolymer (PMMA-b-PCL-NAC) was prepared using the previously described one-pot photoinduced sequential CuAAC/thiol-ene double click procedure. PMMA-b-PCL-NAC had previously shown good applicability as a matrix for cell adhesion of cells from the Vero cell line (African green monkey kidney epithelial). Here, in this work, PMMA-b-PCL-NAC served as an excellent immobilization matrix for biomolecule conjugation. Covalent binding of RGD (R: arginine, G: glycine, and D: aspartic acid) peptide sequence onto the PMMA-b-PCL-NAC-coated surface was performed via EDC chemistry. RGD-modified PMMA-b-PCL-NAC (PMMA-b-PCL-NAC-RGD) as a non-toxic cell proliferation platform was used for selective "integrin αvβ3-mediated cell adhesion and biosensing studies. Both optical and electrochemical techniques were used to monitor the adhesion differences between "integrin αvβ3" receptor positive and negative cell lines on to the designed biofunctional surfaces.
authors.affiliation: Department of Biotechnology, Graduate School of Natural and Applied Sciences, Ege University, 35100, Izmir, Turkey.
authors.forename: Gizem
authors.initials: G
authors.lastname: Oyman Eyrilmez
authors.affiliation: Faculty of Science and Letters, Department of Chemistry, Istanbul Technical University, Maslak, 34469, Istanbul, Turkey.
authors.forename: Sean
authors.initials: S
authors.lastname: Doran
authors.affiliation: Faculty of Science and Letters, Department of Chemistry, Istanbul Technical University, Maslak, 34469, Istanbul, Turkey.
authors.forename: Eljesa
authors.initials: E
authors.lastname: Murtezi
authors.affiliation: Faculty of Science, Department of Biochemistry, Ege University, 35100, Izmir, Turkey.
authors.forename: Bilal
authors.initials: B
authors.lastname: Demir
authors.affiliation: Faculty of Science, Department of Biochemistry, Ege University, 35100, Izmir, Turkey.|Institute on Drug Abuse, Toxicology and Pharmaceutical Science, Ege University, 35100, Izmir, Turkey.
authors.forename: Dilek
authors.initials: D
authors.lastname: Odaci Demirkol
authors.affiliation: Faculty of Medicine, Psychiatry Department, Ege University, 35100, Izmir, Turkey.
authors.forename: Hakan
authors.initials: H
authors.lastname: Coskunol
authors.affiliation: Faculty of Science, Department of Biochemistry, Ege University, 35100, Izmir, Turkey. suna.timur@ege.edu.tr.|Institute on Drug Abuse, Toxicology and Pharmaceutical Science, Ege University, 35100, Izmir, Turkey. suna.timur@ege.edu.tr.
authors.forename: Suna
authors.initials: S
authors.lastname: Timur
authors.affiliation: Faculty of Science and Letters, Department of Chemistry, Istanbul Technical University, Maslak, 34469, Istanbul, Turkey. yusuf@itu.edu.tr.|Faculty of Science, Chemistry Department, Center of Excellence for Advanced Materials Research (CEAMR), King Abdulaziz University, PO Box 80203, Jeddah, 21589, Saudi Arabia. yusuf@itu.edu.tr.
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Yagci
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D057930:Click Chemistry; D006801:Humans; D015603:Keratinocytes / Q000502:physiology; D008768:Methylmethacrylates / Q000737:chemistry*; D009842:Oligopeptides / Q000378:metabolism*; D011091:Polyesters / Q000737:chemistry*; D011485:Protein Binding; D013499:Surface Properties; D054457:Tissue Scaffolds
chemical_list: D008768:Methylmethacrylates; D009842:Oligopeptides; D011091:Polyesters; C000608856:poly(methyl methacrylate)-b-polycaprolactone-N-acetylcysteine; C047981:arginyl-glycyl-aspartic acid
keywords: cell adhesion; cell sensing; electrochemical biosensor; selective surfaces