title: In vitro permeability of silver nanoparticles through porcine oromucosal membrane.
abstract: Silver nanoparticles (AgNPs) can come in contact with human oral mucosa due to their wide use in food industry and hygiene devices. We evaluate transmucosal absorption of 19 nm AgNPs using excised porcine buccal mucosa applied on Franz diffusion cells. Two donor solutions were used: one containing AgNPs (0.5 g/L) and one derived from the ultrafiltration of the former and containing only Ag in its soluble form. Experiments were carried out separately for 4 h. Silver flux permeation was demonstrated through oral mucosa, showing similar values for AgNPs (6.8±4.5 ng cm(-2) h(-1)) and Ag ions (5.2±4.3 ng cm(-2) h(-1)). Our study demonstrates that silver can permeate the oromucosal barrier and that absorption is substantially due to Ag ions, since no permeation difference was found using the two solutions. Mucosal absorption has to be considered in further risk assessment studies.
authors.affiliation: Clinical Unit of Occupational Medicine, Department of Medical Sciences, University of Trieste, Via della Pietà 19, 1-34100 Trieste, Italy.
authors.forename: Marcella
authors.initials: M
authors.lastname: Mauro
authors.affiliation: Department of Chemical and Pharmaceutical Sciences, University of Trieste, via Giorgieri, 1-34127 Trieste, Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Crosera
authors.affiliation: Department of Chemical and Pharmaceutical Sciences, University of Trieste, via Giorgieri, 1-34127 Trieste, Italy.
authors.forename: Carlotta
authors.initials: C
authors.lastname: Bianco
authors.affiliation: Clinical Unit of Occupational Medicine, Department of Medical Sciences, University of Trieste, Via della Pietà 19, 1-34100 Trieste, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Bellomo
authors.affiliation: Clinical Unit of Occupational Medicine, Department of Medical Sciences, University of Trieste, Via della Pietà 19, 1-34100 Trieste, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Bovenzi
authors.affiliation: Department of Chemical and Pharmaceutical Sciences, University of Trieste, via Giorgieri, 1-34127 Trieste, Italy.
authors.forename: Gianpiero
authors.initials: G
authors.lastname: Adami
authors.affiliation: Clinical Unit of Occupational Medicine, Department of Medical Sciences, University of Trieste, Via della Pietà 19, 1-34100 Trieste, Italy. Electronic address: larese@units.it.
authors.forename: Francesca Larese
authors.initials: FL
authors.lastname: Filon
mesh_terms: D000818:Animals; D003102:Colloids; D066298:In Vitro Techniques; D053768:Metal Nanoparticles; D009061:Mouth Mucosa / Q000378:metabolism*; D010539:Permeability; D012834:Silver / Q000737:chemistry*; D013552:Swine
chemical_list: D003102:Colloids; D012834:Silver
keywords: Franz cells; In vitro; Mucosal membrane; Permeation.; Silver nanoparticles