title: A functional HOTAIR rs920778 polymorphism does not contributes to gastric cancer in a Turkish population: a case-control study.
abstract: An aberrant up-regulation of HOX transcript antisense intergenic RNA (HOTAIR), a long non-coding RNA (lncRNA), is associated with human cancers including gastric cancer (GC) and worse clinicopathological features. A naturally occurring functional single nucleotide polymorphism (SNP) rs920,778 (C→T) in the intronic enhancer of HOTAIR gene has been demonstrated to affect HOTAIR expression and cancer susceptibility. To investigate the association of the HOTAIR rs920778 polymorphism on the risk of GC susceptibility in Turkish population, a hospital-based case-control study was carried out consisting of 104 GC and 209 healthy control subjects matched on age and gender. The genotype frequency of HOTAIR rs920778 polymorphism was determined by using TaqMan Real-Time Polymerase Chain Reaction. No statistically significant differences were found in the allele or genotype distributions of the HOTAIR rs920778 polymorphism among GC and healthy control subjects (P > 0.05). Our results demonstrate that the HOTAIR rs920778 polymorphism has not been in any major role in genetic susceptibility to gastric carcinogenesis, at least in the population studied here. Independent studies are needed to validate our findings in a larger series, as well as in patients of different ethnic origins.
authors.affiliation: Department of Nursing, Adıyaman School of Health, Adıyaman University, 02040, Adıyaman, Turkey. slymnbyrm81@gmail.com.
authors.forename: Süleyman
authors.initials: S
authors.lastname: Bayram
authors.affiliation: Department of Gastroenterology, Education and Research Hospital, Adıyaman University, 02040, Adıyaman, Turkey.
authors.forename: Yakup
authors.initials: Y
authors.lastname: Ülger
authors.affiliation: Department of Medical Oncology, Medical Faculty, Mustafa Kemal University, 31000, Hatay, Turkey.
authors.forename: Ahmet Taner
authors.initials: AT
authors.lastname: Sümbül
authors.affiliation: Department of Gastroenterology, Isparta State Hospital, 32100, Isparta, Turkey.
authors.forename: Berrin Yalınbaş
authors.initials: BY
authors.lastname: Kaya
authors.affiliation: Department of General Surgery, Medical Faculty, Çukurova University, 01330, Adana, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Rencüzoğulları
authors.affiliation: Vocational School of Health Services, Adıyaman Univesity, 02040, Adıyaman, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Genç
authors.affiliation: Department of Biology, Faculty of Science and Letters, Adıyaman University, 02040, Adıyaman, Turkey.
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Sevgiler
authors.affiliation: Department of Biology, Institute of Science, Adıyaman University, 02040, Adıyaman, Turkey.
authors.forename: Onur
authors.initials: O
authors.lastname: Bozkurt
authors.affiliation: Department of Biology, Faculty of Science and Letters, Adıyaman University, 02040, Adıyaman, Turkey.
authors.forename: Eyyüp
authors.initials: E
authors.lastname: Rencüzoğulları
mesh_terms: D016022:Case-Control Studies; D005260:Female; D005500:Follow-Up Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011379:Prognosis; D062085:RNA, Long Noncoding / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000453:epidemiology / Q000235:genetics*; D014421:Turkey / Q000453:epidemiology
chemical_list: C561566:HOTAIR long untranslated RNA, human; D062085:RNA, Long Noncoding
keywords: Gastric cancer; Genetic susceptibility; HOTAIR; HOTAIR rs920778 polymorphism; lncRNA