title: One-step purification and concentration of DNA in porous membranes for point-of-care applications.
abstract: The emergence of rapid, user-friendly, point-of-care (POC) diagnostic systems is paving the way for better disease diagnosis and control. Lately, there has been a strong emphasis on developing molecular-based diagnostics due to their potential for greatly increased sensitivity and specificity. One of the most critical steps in developing practical diagnostic systems is the ability to perform sample preparation, especially the purification of nucleic acids (NA), at the POC. As such, we have developed a simple-to-use, inexpensive, and disposable sample preparation system for in-membrane purification and concentration of NAs. This system couples lateral flow in a porous membrane with chitosan, a linear polysaccharide that captures NAs via anion exchange chromatography. The system can also substantially concentrate the NAs. The combination of these capabilities can be used on a wide range of sample types, which are prepared for use in downstream processes, such as qPCR, without further purification.
authors.affiliation: University of Washington, Department of Bioengineering, 3720 15th Ave NE, Seattle, WA 98195, USA. sabyrnes@uw.edu yagerp@uw.edu.
authors.forename: S A
authors.initials: SA
authors.lastname: Byrnes
authors.forename: J D
authors.initials: JD
authors.lastname: Bishop
authors.forename: L
authors.initials: L
authors.lastname: Lafleur
authors.forename: J R
authors.initials: JR
authors.lastname: Buser
authors.forename: B
authors.initials: B
authors.lastname: Lutz
authors.forename: P
authors.initials: P
authors.lastname: Yager
mesh_terms: D048271:Chitosan; D004247:DNA / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D008567:Membranes, Artificial; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D019095:Point-of-Care Systems; D016062:Porosity
chemical_list: D008567:Membranes, Artificial; D004247:DNA; D048271:Chitosan