title: Time-resolved gene expression profiling during reprogramming of C/EBPα-pulsed B cells into iPS cells.
abstract: The reprogramming of somatic cells to induced pluripotent stem cells (iPSCs) is lengthy and inefficient. The development of a reprogramming system that allows rapid and synchronous reprogramming to pluripotency is imperative for understanding the mechanism of iPSC formation and for future therapeutic applications. We have recently reported that a short expression in mouse primary B cells of the transcription factor C/EBPα before the induction of pluripotency factors increases the iPSC reprogramming efficiency >100-fold, involving 95% of the cells within a week. Here we present a dataset containing the time course of gene expression during this process as determined by microarray and RNA-seq techniques.
authors.affiliation: Gene Regulation, Stem Cells and Cancer Programme, Centre for Genomic Regulation (CRG) , Dr Aiguader 88, 08003, Barcelona , Spain ; Universitat Pompeu Fabra (UPF) , Dr Aiguader 88, 08003, Barcelona, Spain.
authors.forename: Bruno
authors.initials: B
authors.lastname: Di Stefano
authors.affiliation: École Normale Supérieure, Institut de Biologie de l'ENS , 45 Rue d'Ulm, Paris, F-75005, France ; Inserm, U1024 , Paris, F-75005, France ; CNRS, UMR 8197 , Paris, F-75005, France.
authors.forename: Samuel
authors.initials: S
authors.lastname: Collombet
authors.affiliation: Gene Regulation, Stem Cells and Cancer Programme, Centre for Genomic Regulation (CRG) , Dr Aiguader 88, 08003, Barcelona , Spain ; Universitat Pompeu Fabra (UPF) , Dr Aiguader 88, 08003, Barcelona, Spain ; Institució Catalana de Recerca i Estudis Avançats (ICREA), Passeig Lluis Companys 23 , 08010, Barcelona, Spain.
authors.forename: Thomas
authors.initials: T
authors.lastname: Graf
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000502:physiology; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics; D065150:Cellular Reprogramming / Q000235:genetics*; D015870:Gene Expression; D020869:Gene Expression Profiling; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000502:physiology; D051379:Mice; D046228:Microarray Analysis
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins; C530718:CEBPA protein, mouse