title: C-di-GMP regulates Pseudomonas aeruginosa stress response to tellurite during both planktonic and biofilm modes of growth.
abstract: Stress response plays an important role on microbial adaptation under hostile environmental conditions. It is generally unclear how the signaling transduction pathway mediates a stress response in planktonic and biofilm modes of microbial communities simultaneously. Here, we showed that metalloid tellurite (TeO3(2-)) exposure induced the intracellular content of the secondary messenger cyclic di-GMP (c-di-GMP) of Pseudomonas aeruginosa. Two diguanylate cyclases (DGCs), SadC and SiaD, were responsible for the increased intracellular content of c-di-GMP. Enhanced c-di-GMP levels by TeO3(2-) further increased P. aeruginosa biofilm formation and resistance to TeO3(2-). P. aeruginosa ΔsadCΔsiaD and PAO1/p(lac)-yhjH mutants with low intracellular c-di-GMP content were more sensitive to TeO3(2-) exposure and had low relative fitness compared to the wild-type PAO1 planktonic and biofilm cultures exposed to TeO3(2-). Our study provided evidence that c-di-GMP level can play an important role in mediating stress response in microbial communities during both planktonic and biofilm modes of growth.
authors.affiliation: 1] Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Singapore 637551 [2] NUS Graduate School of Integrative Sciences and Engineering, National University of Singapore, Singapore 117543.
authors.forename: Song Lin
authors.initials: SL
authors.lastname: Chua
authors.affiliation: 1] Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Singapore 637551 [2] Interdisciplinary Graduate School, Nanyang Technological University, Singapore 637551.
authors.forename: Krishnakumar
authors.initials: K
authors.lastname: Sivakumar
authors.affiliation: Costerton Biofilm Center, Department of International Health, Immunology and Microbiology, University of Copenhagen, 2200 København N, Denmark.
authors.forename: Morten
authors.initials: M
authors.lastname: Rybtke
authors.affiliation: Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Singapore 637551.
authors.forename: Mingjun
authors.initials: M
authors.lastname: Yuan
authors.affiliation: Costerton Biofilm Center, Department of International Health, Immunology and Microbiology, University of Copenhagen, 2200 København N, Denmark.
authors.forename: Jens Bo
authors.initials: JB
authors.lastname: Andersen
authors.affiliation: Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Singapore 637551.
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Nielsen
authors.affiliation: 1] Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Singapore 637551 [2] Costerton Biofilm Center, Department of International Health, Immunology and Microbiology, University of Copenhagen, 2200 København N, Denmark.
authors.forename: Michael
authors.initials: M
authors.lastname: Givskov
authors.affiliation: Costerton Biofilm Center, Department of International Health, Immunology and Microbiology, University of Copenhagen, 2200 København N, Denmark.
authors.forename: Tim
authors.initials: T
authors.lastname: Tolker-Nielsen
authors.affiliation: 1] Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Singapore 637551 [2] School of Civil and Environmental Engineering, Nanyang Technological University, Singapore 639798.
authors.forename: Bin
authors.initials: B
authors.lastname: Cao
authors.affiliation: 1] Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Singapore 637551 [2] Center for Marine Bio-Innovation and School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney 2052, Australia.
authors.forename: Staffan
authors.initials: S
authors.lastname: Kjelleberg
authors.affiliation: 1] Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Singapore 637551 [2] School of Biological Sciences, Nanyang Technological University, Singapore 637551.
authors.forename: Liang
authors.initials: L
authors.lastname: Yang
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D006152:Cyclic GMP / Q000031:analogs & derivatives* / Q000378:metabolism; D024881:Drug Resistance, Bacterial; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D019761:Phosphorus-Oxygen Lyases / Q000235:genetics / Q000378:metabolism; D020543:Proteome / Q000032:analysis; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013052:Spectrometry, X-Ray Emission; D013691:Tellurium / Q000633:toxicity*
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D020543:Proteome; D017382:Reactive Oxygen Species; C062025:bis(3',5')-cyclic diguanylic acid; D019761:Phosphorus-Oxygen Lyases; C060720:diguanylate cyclase; D006152:Cyclic GMP; C026660:tellurous acid; D013691:Tellurium