title: [Virological characteristics of influenza A (H3N2) virus in mainland China during 2013-2014].
abstract: To analyze the antigenic and genetic characteristics of the influenza A (H3N2) virus in mainland China during the surveillance year of 2013-2014, the antigenic characteristics of H3N2 virus were analyzed using reference ferret anti-sera. The nucleotide sequences of the viruses were determined by Sanger dideoxy sequencing, phylogenetic trees were constructed with the neighbor-joining method, and the genetic characteristics of the viruses were determined in comparison to current vaccine strains. The results showed that most of the H3N2 viruses were antigenically closely related to the A/Victoria/361/2011 vaccine strain cell-propagated prototype virus (99.6%). Using the A/Texas/50/2012 egg isolate as the reference antigen, 15.1% of the viruses were found to be closely antigenically related to it, while 11.9% of strains were closely antigenically related to the egg-propagated epidemic strain, A/Shanghai-Changning/1507/2012. Phylogenetic analysis of HA genes indicated that the A(H3N2) viruses in this surveillance year were in the same clade, but no drug resistant mutation was identified in the NA genes. During the 2013-2014 influenza surveillance year, no significant genetic change was detected in either the HA or NA genes of the A(H3N2) viruses, while significant mutations were found in egg isolates resulting from their adaptation during propagation in eggs. The antigenic and genetic changes should be investigated in a timely manner to enable the selection of an appropriate vaccine strain in China.
authors.forename: Xiyan
authors.initials: X
authors.lastname: Li
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Cheng
authors.forename: Minju
authors.initials: M
authors.lastname: Tan
authors.forename: Weijuan
authors.initials: W
authors.lastname: Huang
authors.forename: Junfeng
authors.initials: J
authors.lastname: Guo
authors.forename: Hejiang
authors.initials: H
authors.lastname: Wei
authors.forename: Ning
authors.initials: N
authors.lastname: Xiao
authors.forename: Yu
authors.initials: Y
authors.lastname: Lan
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhao
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.forename: Zhao
authors.initials: Z
authors.lastname: Wang
authors.forename: Dayan
authors.initials: D
authors.lastname: Wang
authors.forename: Yuelong
authors.initials: Y
authors.lastname: Shu
mesh_terms: D000818:Animals; D000940:Antigenic Variation; D001483:Base Sequence; D002642:Chick Embryo; D002681:China; D014644:Genetic Variation; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000276:immunology; D006801:Humans; D053122:Influenza A Virus, H3N2 Subtype / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D007251:Influenza, Human / Q000821:virology*; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus