title: LC-MS-based metabolite profiling of methanolic extracts from the medicinal and aromatic species Mentha pulegium and Origanum majorana.
abstract: BACKGROUND
  There has been increasing interest dedicated to the phenolic compounds with a view to their antioxidant and healthy properties. Recent studies have focused on plants from the Lamiaceae family with special interest in phenolic compounds antioxidant potential.
  
  
  OBJECTIVE
  The metabolite profile of methanolic extracts from two Lamiacea medicinal plants was investigated.
  
  
  METHODS
  Mentha pulegium and Origanum majorana methanolic extracts were analysed using reversed-phase ultra-high-performance liquid chromatography (RP-UHPLC) coupled to electrospray ionisation quadrupole time-of-flight mass spectrometry (ESI-QTOF-MS) detection in the negative ion mode.
  
  
  RESULTS
  A total of 85 metabolites were characterised from different families, such as organic acids and derivatives, amino acids and derivatives, nucleosides, phenolic compounds as well as other polar metabolites, by using the MS and MS/MS information provided by the QTOF-MS. However, the total phenols and flavonoids were also quantified spectrophotometrically and they registered higher amounts in Mentha pulegium than in Origanum majorana extract. Gallocatechin was the major compound in M. pulegium extract whereas quercetin dimethyl ether, jaceidin and dihydrokaempferide were the major ones in O. majorana extract.
  
  
  CONCLUSIONS
  The distribution of phenolic compounds in the methanolic extract showed a variation among studied plants. Mentha pulegium can be considered as a source of gallocatechin.
authors.affiliation: Laboratoire de Biotechnologie de l'Olivier, Centre de Biotechnologie de Borj Cedria, BP 901, 2050, Hammam-Lif, Tunisia.
authors.forename: Amani
authors.initials: A
authors.lastname: Taamalli
authors.affiliation: Department of Analytical Chemistry, University of Granada, Avda. Fuentenueva s/n, 18071, Granada, Spain.|Functional Food Research and Development Centre (CIDAF), Health-Science Technological Park, Avda. Del Conocimiento 3, 18016, Granada, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Arráez-Román
authors.affiliation: Laboratoire de Biotechnologie de l'Olivier, Centre de Biotechnologie de Borj Cedria, BP 901, 2050, Hammam-Lif, Tunisia.
authors.forename: Leila
authors.initials: L
authors.lastname: Abaza
authors.affiliation: Food Technology Study Programme, Life Sciences Faculty, Surya University, Gedung 01 Scientia Business Park, Jl. Boulevard Gading Serpong Blok O/1, SummareconSerpong, Tangerang, 15810, Banten, Indonesia.|Centre for Functional Food, Surya University, Gedung 01 Scientia Business Park, Jl. Boulevard Gading Serpong Blok O/1, Summarecon Serpong, Tangerang, 15810, Banten, Indonesia.
authors.forename: Ihsan
authors.initials: I
authors.lastname: Iswaldi
authors.affiliation: Department of Analytical Chemistry, University of Granada, Avda. Fuentenueva s/n, 18071, Granada, Spain.|Functional Food Research and Development Centre (CIDAF), Health-Science Technological Park, Avda. Del Conocimiento 3, 18016, Granada, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Fernández-Gutiérrez
authors.affiliation: Laboratoire de Biotechnologie de l'Olivier, Centre de Biotechnologie de Borj Cedria, BP 901, 2050, Hammam-Lif, Tunisia.
authors.forename: Mokhtar
authors.initials: M
authors.lastname: Zarrouk
authors.affiliation: Department of Analytical Chemistry, University of Granada, Avda. Fuentenueva s/n, 18071, Granada, Spain.|Functional Food Research and Development Centre (CIDAF), Health-Science Technological Park, Avda. Del Conocimiento 3, 18016, Granada, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Segura-Carretero
mesh_terms: D002392:Catechin / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods; D006841:Hydrocarbons, Aromatic / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D013058:Mass Spectrometry / Q000379:methods*; D031335:Mentha pulegium / Q000737:chemistry* / Q000378:metabolism; D000432:Methanol / Q000737:chemistry; D031341:Origanum / Q000737:chemistry* / Q000378:metabolism; D035261:Plant Components, Aerial / Q000737:chemistry / Q000378:metabolism; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry / Q000378:metabolism; D011794:Quercetin / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D013045:Species Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D014315:Triterpenes / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D006841:Hydrocarbons, Aromatic; D010936:Plant Extracts; D014315:Triterpenes; C548020:jaceidin; D002392:Catechin; D011794:Quercetin; C057580:gallocatechol; D000432:Methanol
keywords: Lamiaceae; QTOF-MS; RP-UHPLC; phenolic compounds