title: An aptasensor for selective, sensitive and fast detection of lead(II) based on polyethyleneimine and gold nanoparticles.
abstract: Lead (Pb), as a major environmental contaminant, could be harmful to humans when inhaled or ingested. In this study, we developed a sensitive, selective and fast colorimetric aptasensor for Pb(+2) based on polyethylenimine (PEI) and gold nanoparticles (AuNPs). In the absence of Pb(+2), aptamer binds to PEI. So the well-dispersed AuNPs remain stable with a wine-red color. Upon the addition of Pb(+2), a conformational change happens and a G-quadruplex aptamer/Pb(+2) complex is formed, leading to the aggregation of AuNPs and a color change to blue. This sensor showed a high selectivity toward Pb(+2) with a limit of detection (LOD) as low as 702pM. Moreover, our fabricated sensor was successfully applied for Pb(+2) detection in rat serum and tap water.
authors.affiliation: Targeted Drug Delivery Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Seyed Mohammad
authors.initials: SM
authors.lastname: Taghdisi
authors.affiliation: Research Institute of Sciences and New Technology, Mashhad, Iran; Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Noor Mohammad
authors.initials: NM
authors.lastname: Danesh
authors.affiliation: Department of Chemistry, Ferdowsi University of Mashhad, Mashhad, Iran.
authors.forename: Parirokh
authors.initials: P
authors.lastname: Lavaee
authors.affiliation: Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: ramezanim@mums.ac.ir.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Ramezani
authors.affiliation: Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: abnouskh@mums.ac.ir.
authors.forename: Khalil
authors.initials: K
authors.lastname: Abnous
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods; D003124:Colorimetry / Q000379:methods*; D004784:Environmental Monitoring / Q000379:methods; D006046:Gold / Q000737:chemistry*; D007854:Lead / Q000032:analysis* / Q000097:blood; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D008956:Models, Chemical; D011094:Polyethyleneimine / Q000378:metabolism*; D051381:Rats; D014874:Water Pollutants, Chemical / Q000032:analysis
chemical_list: D052157:Aptamers, Nucleotide; D014874:Water Pollutants, Chemical; D007854:Lead; D006046:Gold; D011094:Polyethyleneimine
keywords: Aptamer; Gold nanoparticles; Lead; Polyethylenimine; Sensor