title: Phototherapy with blue and green mixed-light is as effective against unconjugated jaundice as blue light and reduces oxidative stress in the Gunn rat model.
abstract: OBJECTIVE
  Phototherapy using blue light-emitting diodes (LED) is effective against neonatal jaundice. However, green light phototherapy also reduces unconjugated jaundice. We aimed to determine whether mixed blue and green light can relieve jaundice with minimal oxidative stress as effectively as either blue or green light alone in a rat model.
  
  
  METHODS
  Gunn rats were exposed to phototherapy with blue (420-520 nm), filtered blue (FB; 440-520 nm without<440-nm wavelengths, FB50 (half the irradiance of filtered blue), mixed (filtered 50% blue and 50% green), and green (490-590 nm) LED irradiation for 24h. The effects of phototherapy are expressed as ratios of serum total (TB) and unbound (UB) bilirubin before and after exposure to each LED. Urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) was measured by HPLC before and after exposure to each LED to determine photo-oxidative stress.
  
  
  RESULTS
  Values < 1.00 indicate effective phototherapy. The ratios of TB and UB were decreased to 0.85, 0.89, 1.07, 0.90, and 1.04, and 0.85, 0.94, 0.93, 0.89, and 1.09 after exposure to blue, filtered blue, FB50, and filtered blue mixed with green LED, respectively. In contrast, urinary 8-OHdG increased to 2.03, 1.25, 0.96, 1.36, 1.31, and 1.23 after exposure to blue, filtered blue, FB50, mixed, green LED, and control, indicating side-effects (> 1.00), respectively.
  
  
  CONCLUSIONS
  Blue plus green phototherapy is as effective as blue phototherapy and it attenuates irradiation-induced oxidative stress.
  
  
  CONCLUSIONS
  Combined blue and green spectra might be effective against neonatal hyperbilirubinemia.
authors.affiliation: Division of Neonatal Intensive Care, Nara Medical University Hospital, Center of Perinatal Medicine, 840 Shijyo-cho, Kashihara, Nara 634-8522, Japan. Electronic address: rmbsh274@yahoo.co.jp.
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Uchida
authors.affiliation: Project Promotion Department, Technology and Engineering Division, Ushio Incorporated, 1194 Sazuchi, Bessyo-cho, Himeji, Hyogo 671-0224, Japan.
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Morimoto
authors.affiliation: Central Clinical Laboratory, Nara Medical University Hospital, 840 Shijyo-cho, Kashihara, Nara 634-8522, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Uchiike
authors.affiliation: Division of Neonatal Intensive Care, Nara Medical University Hospital, Center of Perinatal Medicine, 840 Shijyo-cho, Kashihara, Nara 634-8522, Japan.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Kamamoto
authors.affiliation: Division of Neonatal Intensive Care, Nara Medical University Hospital, Center of Perinatal Medicine, 840 Shijyo-cho, Kashihara, Nara 634-8522, Japan.
authors.forename: Tamaki
authors.initials: T
authors.lastname: Hayashi
authors.affiliation: Division of Neonatal Intensive Care, Nara Medical University Hospital, Center of Perinatal Medicine, 840 Shijyo-cho, Kashihara, Nara 634-8522, Japan.
authors.forename: Ikuyo
authors.initials: I
authors.lastname: Arai
authors.affiliation: Division of Neonatal Intensive Care, Nara Medical University Hospital, Center of Perinatal Medicine, 840 Shijyo-cho, Kashihara, Nara 634-8522, Japan.
authors.forename: Toshiya
authors.initials: T
authors.lastname: Nishikubo
authors.affiliation: Division of Neonatal Intensive Care, Nara Medical University Hospital, Center of Perinatal Medicine, 840 Shijyo-cho, Kashihara, Nara 634-8522, Japan.
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Takahashi
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D001663:Bilirubin / Q000097:blood*; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000652:urine; D004195:Disease Models, Animal; D006801:Humans; D007565:Jaundice / Q000097:blood / Q000628:therapy* / Q000652:urine; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*; D010789:Phototherapy / Q000379:methods*; D051381:Rats; D011911:Rats, Gunn; D016896:Treatment Outcome
chemical_list: D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; D001663:Bilirubin
keywords: Hyperbilirubinemia; Light emitting diode; Neonate; Side effects; Unbound bilirubin