title: A label-free impedimetric immunosensor for direct determination of the textile dye Disperse Orange 1.
abstract: A strategy for a label-free impedimetric immunosensor is described for detection of the textile dye Disperse Orange 1 (DO1). The compounds 1,12-diaminododecane (DADD) and then 1,7-diaminoheptane (DAH) were firstly successively grafted onto a glassy carbon electrode (GCE) surface by electro-oxidation of one amino group, while the other terminal amino group was modified with the antibody anti-DO1. The construction process of the immunosensor was characterized by cyclic voltammetry, electrochemical impedance spectroscopy and capacitance measurements. The electron transfer resistance (Rct) exhibited an effective response to the affinity between the immobilized antibody and the antigen in solution. The linear range for the target compound was from 5.0 nmol L(-1) to 0.5 μmol L(-1) (R=0.9980), and the limit of detection (LOD) was 7.56 nmol L(-1). The proposed impedimetric immunosensor has the advantages of simplicity, cost-effectiveness, and sensitivity.
authors.affiliation: College of Chemistry and Chemical Engineering, Hubei University, 368 Youyi Road, Wuhan CN 430062, Hubei Province, China. Electronic address: 0804yangjing@163.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: UNESP - Universidade Estadual Paulista, Institute of Chemistry, Rua Prof. Francisco Degni 55, 14800-060 Araraquara, SP, Brazil. Electronic address: carolgomesrocha@hotmail.com.
authors.forename: Carolina Gomes
authors.initials: CG
authors.lastname: da Rocha
authors.affiliation: College of Chemistry and Chemical Engineering, Hubei University, 368 Youyi Road, Wuhan CN 430062, Hubei Province, China. Electronic address: wangsf@hubu.edu.cn.
authors.forename: Shengfu
authors.initials: S
authors.lastname: Wang
authors.affiliation: UNESP - Universidade Estadual Paulista, Institute of Chemistry, Rua Prof. Francisco Degni 55, 14800-060 Araraquara, SP, Brazil. Electronic address: antoferr@iq.unesp.br.
authors.forename: Antonio Aparecido Pupim
authors.initials: AA
authors.lastname: Ferreira
authors.affiliation: UNESP - Universidade Estadual Paulista, Institute of Chemistry, Rua Prof. Francisco Degni 55, 14800-060 Araraquara, SP, Brazil. Electronic address: hidekoy@iq.unesp.br.
authors.forename: Hideko
authors.initials: H
authors.lastname: Yamanaka
mesh_terms: D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D001391:Azo Compounds / Q000032:analysis* / Q000276:immunology; D002244:Carbon / Q000737:chemistry; D004396:Coloring Agents / Q000032:analysis*; D003959:Diamines / Q000737:chemistry; D058266:Dielectric Spectroscopy; D025802:Electric Capacitance; D004566:Electrodes; D057230:Limit of Detection; D013784:Textiles; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D000906:Antibodies; D001391:Azo Compounds; D004396:Coloring Agents; D003959:Diamines; D014874:Water Pollutants, Chemical; C556349:Disperse Orange 1; C016417:1,12-dodecamethylenediamine; C015127:1,7-diaminoheptane; D002244:Carbon
keywords: Antibody immobilization; Disperse Orange 1; Impedimetric immunosensor; Textile dye