title: Restoration of the di-myo-inositol-phosphate pathway in the piezo-hyperthermophilic archaeon Thermococcus barophilus.
abstract: Most Thermococcales accumulate di-myo-inositol-phosphate (DIP) as an organic solute as a response to heat stress. We have studied the accumulation of this osmolyte in the high-hydrostatic pressure adapted hyperthermophile Thermococcus barophilus. We found no accumulation of DIP under any of the stress conditions tested, although this archaeon harbors the 3 DIP synthesis genes. Lack of synthesis is due to the lack of expression of TERMP_01135 coding for the second step of DIP synthesis. In contrast to other species, the T. barophilus synthesis operon is interrupted by a four gene locus, in reverse orientation. Restoring an operon like structure at the DIP locus restored DIP synthesis, but did not have an impact on growth characteristics, suggesting that other mechanisms have evolved in this organism to cope with heat stress.
authors.affiliation: Laboratoire de Géologie de Lyon, CNRS UMR 5276, ENS de Lyon, 46 Allée d'Italie, Lyon France.
authors.forename: Anaïs
authors.initials: A
authors.lastname: Cario
authors.affiliation: Laboratoire de Géologie de Lyon, CNRS UMR 5276, ENS de Lyon, 46 Allée d'Italie, Lyon France.
authors.forename: Alex
authors.initials: A
authors.lastname: Mizgier
authors.affiliation: Université Bretagne Occidentale (UBO), IUEM (Institut Universitaire Européen de la mer) - UMR 6197, Technopole Brest-Iroise, Place Nicolas Copernic, F-29280 Plouzané, France; CNRS, IUEM (Institut Universitaire Européen de la mer) - UMR 6197, Technopole Brest-Iroise, Place Nicolas Copernic, F-29280 Plouzané, France; Ifremer, UMR 6197, Technopole Brest-Iroise, Place Nicolas Copernic, F-29280 Plouzané, France.
authors.forename: Axel
authors.initials: A
authors.lastname: Thiel
authors.affiliation: Université Bretagne Occidentale (UBO), IUEM (Institut Universitaire Européen de la mer) - UMR 6197, Technopole Brest-Iroise, Place Nicolas Copernic, F-29280 Plouzané, France; CNRS, IUEM (Institut Universitaire Européen de la mer) - UMR 6197, Technopole Brest-Iroise, Place Nicolas Copernic, F-29280 Plouzané, France; Ifremer, UMR 6197, Technopole Brest-Iroise, Place Nicolas Copernic, F-29280 Plouzané, France.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Jebbar
authors.affiliation: Laboratoire de Géologie de Lyon, CNRS UMR 5276, ENS de Lyon, 46 Allée d'Italie, Lyon France. Electronic address: poger@ens-lyon.fr.
authors.forename: Phil M
authors.initials: PM
authors.lastname: Oger
mesh_terms: D019843:Archaeal Proteins; D019766:Genes, Archaeal; D006358:Hot Temperature; D007295:Inositol Phosphates / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D016133:Polymerase Chain Reaction; D013312:Stress, Physiological / Q000502:physiology*; D019712:Thermococcus / Q000502:physiology*
chemical_list: D019843:Archaeal Proteins; D007295:Inositol Phosphates; C052128:inositol 3-phosphate
keywords: Heat stress; High hydrostatic pressure; Thermococcus barophilus; di-myo-inositol-phosphate