title: Proteomics analysis after traumatic brain injury in rats: the search for potential biomarkers.
abstract: Many studies of protein expression after traumatic brain injury (TBI) have identified biomarkers for diagnosing or determining the prognosis of TBI. In this study, we searched for additional protein markers of TBI using a fluid perfusion impact device to model TBI in S-D rats. Two-dimensional gel electrophoresis and mass spectrometry were used to identify differentially expressed proteins. After proteomic analysis, we detected 405 and 371 protein spots within a pH range of 3-10 from sham-treated and contused brain cortex, respectively. Eighty protein spots were differentially expressed in the two groups and 20 of these proteins were identified. This study validated the established biomarkers of TBI and identified potential biomarkers that could be examined in future work.
authors.affiliation: Department of Neurosurgery, Sixth People's Hospital, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ding
authors.affiliation: Department of Neurosurgery, Chinese Medicine Hospital of Huangdao District, Shandong, China.
authors.forename: Zhengang
authors.initials: Z
authors.lastname: Ding
authors.affiliation: Department of Neurosurgery, Sixth People's Hospital, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Yuan
authors.affiliation: Department of Neurosurgery, Sixth People's Hospital, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Jianyi
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of Neurosurgery, Sixth People's Hospital, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Neurosurgery, Sixth People's Hospital, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Wenwei
authors.initials: W
authors.lastname: Gao
authors.affiliation: Department of Neurosurgery, Sixth People's Hospital, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Ren
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Sixth People's Hospital, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Department of Neurosurgery, Sixth People's Hospital, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Jiong
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Neurosurgery, Sixth People's Hospital, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Neurosurgery, Sixth People's Hospital, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Hengli
authors.initials: H
authors.lastname: Tian
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D001923:Brain Chemistry; D001930:Brain Injuries / Q000175:diagnosis* / Q000378:metabolism; D002540:Cerebral Cortex / Q000737:chemistry*; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D008297:Male; D013058:Mass Spectrometry; D011379:Prognosis; D040901:Proteomics; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D013997:Time Factors
chemical_list: D015415:Biomarkers