title: Nanosized zeolites as a perspective material for conductometric biosensors creation.
abstract: In this work, the method of enzyme adsorption on different zeolites and mesoporous silica spheres (MSS) was investigated for the creation of conductometric biosensors. The conductometric transducers consisted of gold interdigitated electrodes were placed on the ceramic support. The transducers were modified with zeolites and MSS, and then the enzymes were adsorbed on the transducer surface. Different methods of zeolite attachment to the transducer surface were used; drop coating with heating to 200°C turned out to be the best one. Nanozeolites beta and L, zeolite L, MSS, and silicalite-1 (80 to 450 nm) were tested as the adsorbents for enzyme urease. The biosensors with all tested particles except zeolite L had good analytical characteristics. Silicalite-1 (450 nm) was also used for adsorption of glucose oxidase, acetylcholinesterase, and butyrylcholinesterase. The glucose and acetylcholine biosensors were successfully created, whereas butyrylcholinesterase was not adsorbed on silicalite-1. The enzyme adsorption on zeolites and MSS is simple, quick, well reproducible, does not require use of toxic compounds, and therefore can be recommended for the development of biosensors when these advantages are especially important.
authors.affiliation: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Zabolotnogo Street 150, 03680 Kyiv, Ukraine ; Taras Shevchenko National University of Kyiv, Volodymyrska Street, 64, 01601 Kyiv, Ukraine ; Institute of Analytical Sciences, 5 rue de la Doua, 69100 Villeurbanne, France.
authors.forename: Ivan
authors.initials: I
authors.lastname: Kucherenko
authors.affiliation: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Zabolotnogo Street 150, 03680 Kyiv, Ukraine ; Taras Shevchenko National University of Kyiv, Volodymyrska Street, 64, 01601 Kyiv, Ukraine.
authors.forename: Oleksandr
authors.initials: O
authors.lastname: Soldatkin
authors.affiliation: Central Laboratory, Middle East Technical University, Dumlupinar Bulvari, 1, 06800 Ankara, Turkey.
authors.forename: Berna Ozansoy
authors.initials: BO
authors.lastname: Kasap
authors.affiliation: Central Laboratory, Middle East Technical University, Dumlupinar Bulvari, 1, 06800 Ankara, Turkey.
authors.forename: Salih Kaan
authors.initials: SK
authors.lastname: Kirdeciler
authors.affiliation: Central Laboratory, Middle East Technical University, Dumlupinar Bulvari, 1, 06800 Ankara, Turkey ; Micro and Nanotechnology Department, Middle East Technical University, Dumlupinar Bulvari, 1, 06800 Ankara, Turkey.
authors.forename: Burcu Akata
authors.initials: BA
authors.lastname: Kurc
authors.affiliation: Institute of Analytical Sciences, 5 rue de la Doua, 69100 Villeurbanne, France.
authors.forename: Nicole
authors.initials: N
authors.lastname: Jaffrezic-Renault
authors.affiliation: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Zabolotnogo Street 150, 03680 Kyiv, Ukraine ; Taras Shevchenko National University of Kyiv, Volodymyrska Street, 64, 01601 Kyiv, Ukraine.
authors.forename: Alexei
authors.initials: A
authors.lastname: Soldatkin
authors.affiliation: Institute of Analytical Sciences, 5 rue de la Doua, 69100 Villeurbanne, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Lagarde
authors.affiliation: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Zabolotnogo Street 150, 03680 Kyiv, Ukraine ; Taras Shevchenko National University of Kyiv, Volodymyrska Street, 64, 01601 Kyiv, Ukraine.
authors.forename: Sergei
authors.initials: S
authors.lastname: Dzyadevych
keywords: Acetylcholinesterase; Biosensor; Conductometric transducer; Enzyme adsorption; Mesoporous silica spheres; Silicalite-1; Urease; Zeolite