title: Separation of polyphenols from leaves of Malus hupehensis (Pamp.) Rehder by off-line two-dimensional High Speed Counter-Current Chromatography combined with recycling elution mode.
abstract: In this study, off-line two-dimensional High Speed Counter-Current Chromatography (2D HSCCC) strategy combined with recycling elution mode was developed to isolate compounds from the ethyl acetate extract of a common green tea--leaves of Malus hupehensis (Pamp.) Rehder. In the orthogonal separation system, a conventional HSCCC was employed for the first dimension and two recycling HSCCCs were used for the second in parallel. Using a solvent system consisting of n-hexane-ethyl acetate-methanol-water (1:4:0.6:4.4, v/v) in the first and second dimension, four compounds including 3-hydroxy-phlorizin (1), phloretin (2), avicularin (3) and kaempferol 3-O-β-D-glucoside (4) were obtained. The purities of these four compounds were all over 95.0% as determined by HPLC. And their structures were all identified through UV, MS and (1)H NMR. It has been demonstrated that the combination of off-line 2D HSCCC with recycling elution mode is an efficient technique to isolate compounds with similar polarities in natural products.
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
authors.forename: Hualiang
authors.initials: H
authors.lastname: Zeng
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
authors.forename: Shujing
authors.initials: S
authors.lastname: Jiang
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
authors.forename: Fuzhu
authors.initials: F
authors.lastname: Yang
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China; Collaborative Innovation Center of Resource-conserving & Environment-friendly Society and Ecological Civilization, Changsha 410083, China. Electronic address: xqchen@csu.edu.cn.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Chen
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China. Electronic address: hychem@csu.edu.cn.
authors.forename: Hua
authors.initials: H
authors.lastname: Yang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution; D005419:Flavonoids / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006586:Hexanes / Q000737:chemistry; D044949:Kaempferols / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D027845:Malus / Q000737:chemistry*; D009005:Monosaccharides / Q000032:analysis; D010693:Phloretin / Q000032:analysis; D010936:Plant Extracts / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry*; D059808:Polyphenols / Q000032:analysis*; D012997:Solvents / Q000737:chemistry
chemical_list: C115838:4'-dehydroxyphlorizin; D005419:Flavonoids; D006586:Hexanes; D044949:Kaempferols; D009005:Monosaccharides; D010936:Plant Extracts; D059808:Polyphenols; D012997:Solvents; C511963:kaempferol-3-O-glucoside; C026385:n-hexane; C041388:avicularin; D005947:Glucose; D010693:Phloretin
keywords: 3-Hydroxy-phlorizin; Closed-loop recycling; Malus hupehensis (Pamp.) Rehder; Phlorizin; Recycling HSCCC