title: EPR spectroscopy of chlorpromazine-induced free radical formation in normal human melanocytes.
abstract: The purpose of this study was to estimate the effect of chlorpromazine on free radical concentration in HEMn-DP melanocytes using electron paramagnetic resonance (EPR) spectroscopy. It was found that chlorpromazine at concentrations of 1 × 10(-7) and 1 × 10(-6) M contributed to the formation of free radicals (g values ~2) in a dose-dependent manner. The increase in free radical formation was accompanied by an increase in cytotoxicity, as shown by a tetrazolium assay. Homogeneous broadening of EPR lines, slow spin-lattice relaxation processes, and strong dipolar interactions characterized all the tested cellular samples. The performed examination of free radical formation in cells exposed to different chlorpromazine concentrations confirmed the usefulness of electron paramagnetic resonance spectroscopy to determine the effect of a drug on free radical production in a cellular model system in vitro.
authors.affiliation: Chair and Department of Pharmaceutical Chemistry, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Jagiellońska 4, 41-200, Sosnowiec, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Otręba
authors.forename: Magdalena
authors.initials: M
authors.lastname: Zdybel
authors.forename: Barbara
authors.initials: B
authors.lastname: Pilawa
authors.forename: Artur
authors.initials: A
authors.lastname: Beberok
authors.forename: Dorota
authors.initials: D
authors.lastname: Wrześniok
authors.forename: Jakub
authors.initials: J
authors.lastname: Rok
authors.forename: Ewa
authors.initials: E
authors.lastname: Buszman
mesh_terms: D002460:Cell Line; D002746:Chlorpromazine / Q000494:pharmacology*; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals / Q000378:metabolism*; D006801:Humans; D008544:Melanocytes / Q000187:drug effects*
chemical_list: D005609:Free Radicals; D002746:Chlorpromazine