title: Soluble ST2 and IL-33: Potential markers of endometriosis in the Tunisian population.
abstract: Interleukin-33 is an IL-1 family cytokine which signals via its T1/ST2 receptor, and acts as a key regulator of inflammation. This study aims to measure the expression of soluble ST2 (sST2) and IL-33 in endometriosis. We investigated thirty women with laparoscopic and histopathological confirmed endometriosis and 20 control women without pelvic pathology. Peripheral blood mononuclear cells and peritoneal fluid (PF) were assessed for sST2 and IL-33 levels that are measured by sandwich enzyme-linked immunosorbent assay. Peritoneal fluid IL-33 mRNA expression was quantified by real-time reverse transcription polymerase chain reaction assays. We found that IL-33 levels in PF and in serum were significantly higher in patients with endometriosis compared to women without endometriosis (P < 0.05). IL-33 increased levels were significantly more important in PF [10.45 ± 14.33 ng/mL] than in serum [2.68 ± 1.54 ng/mL] from endometriosis patients. Higher levels of IL-33 mRNA expression were detected in PF from patients with endometriosis. Soluble ST2 levels in PF were significantly different between patients [2.96 ± 0.98 ng/mL; P < 0.0001] and controls [0.88 ± 0.076 ng/mL]. Serum sST2 levels were similarly expressed in endometriosis patients and in controls (P > 0.05). Significant correlation was observed between IL-33 and sST2 levels in PF. In conclusion, IL-33 and sST2 values observed in PF were found to correlate with endometriosis severity. Elevated and correlated PF IL-33 and sST2 levels from patients with endometriosis suggested a potential role as surrogate markers of disease activity.
authors.affiliation: Tunis El Manar University, Medicine Faculty of Tunis, Department of Immunology and Histology, Tunisia. Electronic address: marwambarik@gmail.com.
authors.forename: Maroua
authors.initials: M
authors.lastname: Mbarik
authors.affiliation: Tunis El Manar University, Medicine Faculty of Tunis, Department of Immunology and Histology, Tunisia; A. Mami Hospital, Unit Research UR/12SP15 (Homeostasis and cell immune dysfunction), Ariana, Tunisia. Electronic address: kaabachi.wajih@gmail.com.
authors.forename: Wajih
authors.initials: W
authors.lastname: Kaabachi
authors.affiliation: Tunis El Manar University, Medicine Faculty of Tunis, Department of Immunology and Histology, Tunisia. Electronic address: belkis.henidi@yahoo.fr.
authors.forename: Balkis
authors.initials: B
authors.lastname: Henidi
authors.affiliation: Tunis El Manar University, Medicine Faculty of Tunis, Department of Immunology and Histology, Tunisia; A. Mami Hospital, Unit Research UR/12SP15 (Homeostasis and cell immune dysfunction), Ariana, Tunisia. Electronic address: faycalhsassi@yahoo.fr.
authors.forename: Faycal Haj
authors.initials: FH
authors.lastname: Sassi
authors.affiliation: Tunis El Manar University, Medicine Faculty of Tunis, Department of Immunology and Histology, Tunisia; A. Mami Hospital, Unit Research UR/12SP15 (Homeostasis and cell immune dysfunction), Ariana, Tunisia. Electronic address: kamel.hamzaoui@gmail.com.
authors.forename: Kamel
authors.initials: K
authors.lastname: Hamzaoui
mesh_terms: D001202:Ascitic Fluid / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D004715:Endometriosis / Q000175:diagnosis / Q000235:genetics / Q000473:pathology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007249:Inflammation; D000072179:Interleukin-1 Receptor-Like 1 Protein; D000067596:Interleukin-33 / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D011956:Receptors, Cell Surface / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D014416:Tunisia
chemical_list: D015415:Biomarkers; C079241:IL1RL1 protein, human; C506536:IL33 protein, human; D000072179:Interleukin-1 Receptor-Like 1 Protein; D000067596:Interleukin-33; D012333:RNA, Messenger; D011956:Receptors, Cell Surface
keywords: ELISA; Endometriosis; IL-33; Inflammation; RT-qPCR; Soluble ST2