title: Hyaluronic Acid/Poly-l-Lysine Multilayers as Reservoirs for Storage and Release of Small Charged Molecules.
abstract: Polyelectrolyte multilayer films are nowadays very attractive for bioapplications due to their tunable properties and ability to control cellular response. Here we demonstrate that multilayers made of hyaluronic acid and poly-l-lysine act as high-capacity reservoirs for small charged molecules. Strong accumulation within the film is explained by electrostatically driven binding to free charges of polyelectrolytes. Binding and release mechanisms are discussed based on charge balance and polymer dynamics in the film. Our results show that transport of molecules through the film-solution interface limits the release rate. The multilayers might serve as an effective platform for drug delivery and tissue engineering due to high potential for drug loading and controlled release.
authors.affiliation: Fraunhofer IZI-BB, Am Muehlenberg 13, 14476 Potsdam, Germany.|University of Potsdam, Institute for Biochemistry and Biology, Maulbeerallee 2, 14469 Potsdam, Germany.
authors.forename: Vladimir Z
authors.initials: VZ
authors.lastname: Prokopović
authors.affiliation: Fraunhofer IZI-BB, Am Muehlenberg 13, 14476 Potsdam, Germany.
authors.forename: Claus
authors.initials: C
authors.lastname: Duschl
authors.affiliation: Fraunhofer IZI-BB, Am Muehlenberg 13, 14476 Potsdam, Germany. Dmitry.Volodkin@izi-bb.fraunhofer.de.
authors.forename: Dmitry
authors.initials: D
authors.lastname: Volodkin
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry*; D005452:Fluoresceins / Q000737:chemistry*; D006820:Hyaluronic Acid / Q000737:chemistry*; D007700:Kinetics; D018625:Microscopy, Atomic Force; D011107:Polylysine / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D012235:Rhodamines / Q000737:chemistry*
chemical_list: D005452:Fluoresceins; D011108:Polymers; D012235:Rhodamines; C026188:rhodamine 6G; D011107:Polylysine; C024098:6-carboxyfluorescein; D000255:Adenosine Triphosphate; D006820:Hyaluronic Acid
keywords: diffusion; drug delivery; dye; release mechanism