title: Predicting the right spacing between protein immobilization sites on self-assembled monolayers to optimize ligand binding.
abstract: Self-assembled monolayers designed to immobilize capture antibodies are usually prepared using a mixture of functional and inactive linkers. Here, using low molar ratios (1:1 to 1:100) of the two linkers resulted in loss of binding capability of the anti-EGFR (epidermal growth factor receptor) antibody nimotuzumab, as assessed by surface plasmon resonance imaging. We then developed a simple theoretical model to predict the optimal surface density of the functional linker, taking into account the antibody size and linker diameter. A high (1:1000) dilution of the functional linker yielded the best results. As an advantage, this approach does not require chemical modification of the protein.
authors.affiliation: National Center for Nanoscience and Technology, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Javier Batista
authors.initials: JB
authors.lastname: Perez
authors.affiliation: National Center for Nanoscience and Technology, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Deependra
authors.initials: D
authors.lastname: Tyagi
authors.affiliation: National Center for Nanoscience and Technology, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Mo
authors.initials: M
authors.lastname: Yang
authors.affiliation: Center of Molecular Immunology, Havana 16040, Cuba; Biotech Pharmaceutical, Beijing 100176, China.
authors.forename: Loany
authors.initials: L
authors.lastname: Calvo
authors.affiliation: Center of Molecular Immunology, Havana 16040, Cuba; Biotech Pharmaceutical, Beijing 100176, China.
authors.forename: Rolando
authors.initials: R
authors.lastname: Perez
authors.affiliation: Center of Molecular Immunology, Havana 16040, Cuba; Biotech Pharmaceutical, Beijing 100176, China. Electronic address: emoreno@cim.sld.cu.
authors.forename: Ernesto
authors.initials: E
authors.lastname: Moreno
authors.affiliation: National Center for Nanoscience and Technology, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100190, China. Electronic address: jizhu@nanoctr.cn.
authors.forename: Jinsong
authors.initials: J
authors.lastname: Zhu
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry* / Q000276:immunology; D061067:Antibodies, Monoclonal, Humanized / Q000737:chemistry / Q000276:immunology; D066246:ErbB Receptors / Q000276:immunology; D008024:Ligands; D008958:Models, Molecular; D013499:Surface Properties
chemical_list: D055910:Antibodies, Immobilized; D061067:Antibodies, Monoclonal, Humanized; D008024:Ligands; C501466:nimotuzumab; D066246:ErbB Receptors
keywords: Antibody; Nimotuzumab; Protein immobilization; Self-assembled monolayer; Surface plasmon resonance; Theoretical model