title: Salt induced reduction of lysozyme adsorption at charged interfaces.
abstract: A study of lysozyme adsorption below a behenic acid membrane and at the solid-liquid interface between aqueous lysozyme solution and a silicon wafer in the presence of sodium chloride is presented. The salt concentration was varied between 1 mmol L(-1) and 1000 mmol L(-1). X-ray reflectivity data show a clear dependence of the protein adsorption on the salt concentration. Increasing salt concentrations result in a decreased protein adsorption at the interface until a complete suppression at high concentrations is reached. This effect can be attributed to a reduced attractive electrostatic interaction between the positively charged proteins and negatively charged surfaces by charge screening. The measurements at the solid-liquid interfaces show a transition from unoriented order of lysozyme in the adsorbed film to an oriented order with the short protein axis perpendicular to the solid-liquid interface with rising salt concentration.
authors.affiliation: Fakultät Physik/DELTA, TU Dortmund, 44221 Dortmund, Germany.
authors.forename: Holger
authors.initials: H
authors.lastname: Göhring
authors.forename: Michael
authors.initials: M
authors.lastname: Paulus
authors.forename: Paul
authors.initials: P
authors.lastname: Salmen
authors.forename: Florian
authors.initials: F
authors.lastname: Wirkert
authors.forename: Theresa
authors.initials: T
authors.lastname: Kruse
authors.forename: Patrick
authors.initials: P
authors.lastname: Degen
authors.forename: Susan
authors.initials: S
authors.lastname: Stuhr
authors.forename: Heinz
authors.initials: H
authors.lastname: Rehage
authors.forename: Metin
authors.initials: M
authors.lastname: Tolan
mesh_terms: D000327:Adsorption / Q000187:drug effects; D000818:Animals; D004305:Dose-Response Relationship, Drug; D005227:Fatty Acids / Q000737:chemistry; D008567:Membranes, Artificial; D009113:Muramidase / Q000737:chemistry*; D012965:Sodium Chloride / Q000494:pharmacology*; D013499:Surface Properties
chemical_list: D005227:Fatty Acids; D008567:Membranes, Artificial; D012965:Sodium Chloride; D009113:Muramidase; C007547:behenic acid