title: Differential Immune Responses and Protective Effects in Avirulent Mycobacterial Strains Vaccinated BALB/c Mice.
abstract: Screening live mycobacterial vaccine candidates is the important strategy to develop new vaccines against adult tuberculosis (TB). In this study, the immunogenicity and protective efficacy of several avirulent mycobacterial strains including Mycobacterium smegmatis, M. vaccae, M. terrae, M. phlei, M. trivial, and M. tuberculosis H37Ra were compared with M. bovis BCG in BALB/c mice. Our results demonstrated that differential immune responses were induced in different mycobacterial species vaccinated mice. As BCG-vaccinated mice did, M. terrae immunization resulted in Th1-type responses in the lung, as well as splenocytes secreting IFN-γ against a highly conserved mycobacterial antigen Ag85A. M. smegmatis also induced the same splenocytes secreting IFN-γ as BCG and M. terrae did. In addition, M. terrae and M. smegmatis-immunized mice predominantly increased expression of IL-10 and TGF-β in the lung. Most importantly, mice vaccinated with H37Ra and M. vaccae could provide the same protection in the lung against virulent M. tuberculosis challenge as BCG. The result may have important implications in developing adult TB vaccine.
authors.affiliation: Department of Medical Laboratory, Children's Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, 400014, People's Republic of China, laichengliu@126.com.
authors.forename: Laicheng
authors.initials: L
authors.lastname: Liu
authors.forename: Ruiling
authors.initials: R
authors.lastname: Fu
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Yuan
authors.forename: Chunwei
authors.initials: C
authors.lastname: Shi
authors.forename: Shuling
authors.initials: S
authors.lastname: Wang
authors.forename: Xianyu
authors.initials: X
authors.lastname: Lu
authors.forename: Zhao
authors.initials: Z
authors.lastname: Ma
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Zhang
authors.forename: Weiyan
authors.initials: W
authors.lastname: Qin
authors.forename: Xionglin
authors.initials: X
authors.lastname: Fan
mesh_terms: D000217:Acyltransferases / Q000276:immunology; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical / Q000379:methods; D007371:Interferon-gamma / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008168:Lung / Q000276:immunology; D008807:Mice, Inbred BALB C; D009161:Mycobacterium / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D016896:Treatment Outcome; D014376:Tuberculosis / Q000276:immunology / Q000517:prevention & control; D032581:Tuberculosis Vaccines / Q000008:administration & dosage / Q000276:immunology*; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D032581:Tuberculosis Vaccines; D014613:Vaccines, Attenuated; D007371:Interferon-gamma; D000217:Acyltransferases; C121433:antigen 85B, Mycobacterium tuberculosis