title: Vitamin D deficiency contributes to the reduction and impaired function of naïve CD45RA⁺ regulatory T cell in chronic heart failure.
abstract: The effect of vitamin D pertinent to cardiovascular health on the heart itself is considered to shift toward an anti-inflammatory response in chronic heart failure (CHF); however, its underlying mechanism is not completely understood. In this study, we demonstrated that plasma 25(OH)D level, negatively associated with NT-ProBNP, correlated with the decreased Treg in CHF compared to the patients with other cardiovascular diseases and healthy and older donors. Naïve Treg cell (CD4(+)CD45RA(+)Foxp3(lo)T) subset, rather than whole Treg cells, contributes to the reduction of Treg in CHF. 1,25(OH)2D treatment maintained partial expression of CD45RA on CD4(+)T cell after αCD3/CD28 monoclonal antibodies activation and ameliorated the impaired CD4(+)CD45RA(+)T cell function from CHF patients through upregulating Foxp3 expression and IL-10 secretion in vitro. Low level of vitamin D receptor (VDR) was detected in CD4(+)CD45RA(+)T cell of CHF than control, while 1,25(OH)2D treatment increased the VDR expression to exert its immunosuppression on T cell. The results of this study might provide tangible evidence to our knowledge of the impact of vitamin D supplementation on naïve Tregs, which may offer new means of preventing and treating CHF.
authors.affiliation: Department of Laboratory Medicine, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China.
authors.forename: Yan-hui
authors.initials: YH
authors.lastname: Ma
authors.affiliation: Department of Laboratory Medicine, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China.
authors.forename: Yun-lan
authors.initials: YL
authors.lastname: Zhou
authors.affiliation: Department of Laboratory Medicine, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China.
authors.forename: Chao-yan
authors.initials: CY
authors.lastname: Yue
authors.affiliation: Department of Laboratory Medicine, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China.
authors.forename: Guang-hui
authors.initials: GH
authors.lastname: Zhang
authors.affiliation: Department of Laboratory Medicine, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Deng
authors.affiliation: Department of Laboratory Medicine, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China.
authors.forename: Guo-hua
authors.initials: GH
authors.lastname: Xie
authors.affiliation: Department of Internal Cardiology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China.
authors.forename: Wei-ping
authors.initials: WP
authors.lastname: Xu
authors.affiliation: Department of Laboratory Medicine, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China.
authors.forename: Li-song
authors.initials: LS
authors.lastname: Shen
mesh_terms: D015652:25-Hydroxyvitamin D 2 / Q000097:blood / Q000378:metabolism / Q000494:pharmacology*; D000368:Aged; D000893:Anti-Inflammatory Agents / Q000378:metabolism; D015704:CD4 Antigens / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D006333:Heart Failure / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007249:Inflammation / Q000276:immunology; D007371:Interferon-gamma / Q000097:blood; D016753:Interleukin-10 / Q000097:blood; D020381:Interleukin-17 / Q000097:blood; D017493:Leukocyte Common Antigens / Q000378:metabolism; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D058504:Th17 Cells / Q000276:immunology*; D014808:Vitamin D Deficiency / Q000473:pathology*
chemical_list: D000893:Anti-Inflammatory Agents; D015704:CD4 Antigens; C508609:IL10 protein, human; C508717:IL17A protein, human; D020381:Interleukin-17; D016753:Interleukin-10; D015652:25-Hydroxyvitamin D 2; D007371:Interferon-gamma; D017493:Leukocyte Common Antigens