title: Diagnostic ion filtering strategy for chemical characterization of Guge Fengtong Tablet with high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry.
abstract: The present study was designed to characterize the chemical constituents of Guge Fengtong Tablet (GGFTT). Based on the chromatographic retention behavior, fragmentation pathways of chemical components and the published literatures, a diagnostic ion filtering strategy with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (HPLC-ESI-Q-TOF/MS) was established to identify the multiple bioactive constituents of GGFTT. The rapid identification of forty-seven components, including 18 phenolic acids, 8 saponins, 14 gingerol-related compounds, and 7 diarylhepatonoids, was accomplished using this newly developed method. The coupling of HPLC-ESI-Q-TOF/MS with the diagnostic ion filtering strategy was useful and efficient for the in-depth structural elucidation of chemical compounds of GGFTT.
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Su-Ling
authors.initials: SL
authors.lastname: Zeng
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Xin-Guang
authors.initials: XG
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Chang-Jiang-Sheng
authors.initials: CJ
authors.lastname: Lai
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China. Electronic address: liuehu2011@163.com.
authors.forename: E-Hu
authors.initials: EH
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China. Electronic address: liping2004@126.com.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
mesh_terms: D002396:Catechols / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D036381:Diarylheptanoids / Q000302:isolation & purification; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005233:Fatty Alcohols / Q000302:isolation & purification; D062385:Hydroxybenzoates / Q000302:isolation & purification; D012503:Saponins / Q000302:isolation & purification; D021241:Spectrometry, Mass, Electrospray Ionization; D013608:Tablets, Enteric-Coated / Q000737:chemistry; D053719:Tandem Mass Spectrometry
chemical_list: D002396:Catechols; D036381:Diarylheptanoids; D004365:Drugs, Chinese Herbal; D005233:Fatty Alcohols; D062385:Hydroxybenzoates; D012503:Saponins; D013608:Tablets, Enteric-Coated; C540317:Zhengqing Fengtongning; C007845:gingerol; C017616:phenolic acid
keywords: Diagnostic ion filtering strategy; Guge Fengtong Tablet; HPLC-ESI-Q-TOF/MS