title: Milk-soluble formula increases food intake and reduces Il6 expression in elderly rat hypothalami.
abstract: Malnutrition in the elderly is accompanied by several metabolic dysfunctions, especially alterations in energy homeostasis regulation and a loss of insulin responsiveness. Nutritional recommendations aim to enrich food with high protein and energy supplements, and protein composition and lipid quality have been widely studied. Despite the numerous studies that have examined attempts to overcome malnutrition in the elderly through such nutritional supplementation, it is still necessary to study the effects of a combination of protein, lipids, and vitamin D (VitD). This can be done in animal models of elderly malnutrition. In the present study, we investigated the effects of several diet formulae on insulin responsiveness, inflammation, and the hypothalamic expression of key genes that are involved in energy homeostasis control. To mimic elderly malnutrition in humans, elderly Wistar rats were food restricted (R, -50%) for 12 weeks and then refed for 4 weeks with one of four different isocaloric diets: a control diet; a diet where milk soluble protein (MSP) replaced casein; a blend of milk fat, rapeseed, and DHA (MRD); or a full formula (FF) diet that combined MSP and a blend of MRD (FF). All of the refeeding diets contained VitD. We concluded that: (i) food restriction led to the upregulation of insulin receptor in liver and adipose tissue accompanied by increased Tnfα in the hypothalamus; (ii) in all of the refed groups, refeeding led to similar body weight gain during the refeeding period; and (iii) refeeding with MSP and MRD diets induced higher food intake on the fourth week of refeeding, and this increase was associated with reduced hypothalamic interleukin 6 expression.
authors.affiliation: Neuroendocrinologie Moléculaire de la Prise AlimentaireUniversity of Paris-Sud, UMR 8195, F-91405 Orsay, FranceNeuroendocrinologie Moléculaire de la Prise AlimentaireCNRS, Centre de Neurosciences Paris-Sud, UMR 8195, F-91405 Orsay, FranceService NutritionLactalis Recherche et Développement, 8 Fromy, CS 60082, 35240 Retiers, FranceLactalis Nutrition Parc d'Activité de Torcé-secteur Est35370 Torcé, France Neuroendocrinologie Moléculaire de la Prise AlimentaireUniversity of Paris-Sud, UMR 8195, F-91405 Orsay, FranceNeuroendocrinologie Moléculaire de la Prise AlimentaireCNRS, Centre de Neurosciences Paris-Sud, UMR 8195, F-91405 Orsay, FranceService NutritionLactalis Recherche et Développement, 8 Fromy, CS 60082, 35240 Retiers, FranceLactalis Nutrition Parc d'Activité de Torcé-secteur Est35370 Torcé, France.
authors.forename: Hassina
authors.initials: H
authors.lastname: Ould Hamouda
authors.affiliation: Neuroendocrinologie Moléculaire de la Prise AlimentaireUniversity of Paris-Sud, UMR 8195, F-91405 Orsay, FranceNeuroendocrinologie Moléculaire de la Prise AlimentaireCNRS, Centre de Neurosciences Paris-Sud, UMR 8195, F-91405 Orsay, FranceService NutritionLactalis Recherche et Développement, 8 Fromy, CS 60082, 35240 Retiers, FranceLactalis Nutrition Parc d'Activité de Torcé-secteur Est35370 Torcé, France Neuroendocrinologie Moléculaire de la Prise AlimentaireUniversity of Paris-Sud, UMR 8195, F-91405 Orsay, FranceNeuroendocrinologie Moléculaire de la Prise AlimentaireCNRS, Centre de Neurosciences Paris-Sud, UMR 8195, F-91405 Orsay, FranceService NutritionLactalis Recherche et Développement, 8 Fromy, CS 60082, 35240 Retiers, FranceLactalis Nutrition Parc d'Activité de Torcé-secteur Est35370 Torcé, France.
authors.forename: Bernadette
authors.initials: B
authors.lastname: Delplanque
authors.affiliation: Neuroendocrinologie Moléculaire de la Prise AlimentaireUniversity of Paris-Sud, UMR 8195, F-91405 Orsay, FranceNeuroendocrinologie Moléculaire de la Prise AlimentaireCNRS, Centre de Neurosciences Paris-Sud, UMR 8195, F-91405 Orsay, FranceService NutritionLactalis Recherche et Développement, 8 Fromy, CS 60082, 35240 Retiers, FranceLactalis Nutrition Parc d'Activité de Torcé-secteur Est35370 Torcé, France Neuroendocrinologie Moléculaire de la Prise AlimentaireUniversity of Paris-Sud, UMR 8195, F-91405 Orsay, FranceNeuroendocrinologie Moléculaire de la Prise AlimentaireCNRS, Centre de Neurosciences Paris-Sud, UMR 8195, F-91405 Orsay, FranceService NutritionLactalis Recherche et Développement, 8 Fromy, CS 60082, 35240 Retiers, FranceLactalis Nutrition Parc d'Activité de Torcé-secteur Est35370 Torcé, France.
authors.forename: Yacir
authors.initials: Y
authors.lastname: Benomar
authors.affiliation: Neuroendocrinologie Moléculaire de la Prise AlimentaireUniversity of Paris-Sud, UMR 8195, F-91405 Orsay, FranceNeuroendocrinologie Moléculaire de la Prise AlimentaireCNRS, Centre de Neurosciences Paris-Sud, UMR 8195, F-91405 Orsay, FranceService NutritionLactalis Recherche et Développement, 8 Fromy, CS 60082, 35240 Retiers, FranceLactalis Nutrition Parc d'Activité de Torcé-secteur Est35370 Torcé, France Neuroendocrinologie Moléculaire de la Prise AlimentaireUniversity of Paris-Sud, UMR 8195, F-91405 Orsay, FranceNeuroendocrinologie Moléculaire de la Prise AlimentaireCNRS, Centre de Neurosciences Paris-Sud, UMR 8195, F-91405 Orsay, FranceService NutritionLactalis Recherche et Développement, 8 Fromy, CS 60082, 35240 Retiers, FranceLactalis Nutrition Parc d'Activité de Torcé-secteur Est35370 Torcé, France.
authors.forename: Delphine
authors.initials: D
authors.lastname: Crépin
authors.affiliation: Neuroendocrinologie Moléculaire de la Prise AlimentaireUniversity of Paris-Sud, UMR 8195, F-91405 Orsay, FranceNeuroendocrinologie Moléculaire de la Prise AlimentaireCNRS, Centre de Neurosciences Paris-Sud, UMR 8195, F-91405 Orsay, FranceService NutritionLactalis Recherche et Développement, 8 Fromy, CS 60082, 35240 Retiers, FranceLactalis Nutrition Parc d'Activité de Torcé-secteur Est35370 Torcé, France Neuroendocrinologie Moléculaire de la Prise AlimentaireUniversity of Paris-Sud, UMR 8195, F-91405 Orsay, FranceNeuroendocrinologie Moléculaire de la Prise AlimentaireCNRS, Centre de Neurosciences Paris-Sud, UMR 8195, F-91405 Orsay, FranceService NutritionLactalis Recherche et Développement, 8 Fromy, CS 60082, 35240 Retiers, FranceLactalis Nutrition Parc d'Activité de Torcé-secteur Est35370 Torcé, France.
authors.forename: Laure
authors.initials: L
authors.lastname: Riffault
authors.affiliation: Neuroendocrinologie Moléculaire de la Prise AlimentaireUniversity of Paris-Sud, UMR 8195, F-91405 Orsay, FranceNeuroendocrinologie Moléculaire de la Prise AlimentaireCNRS, Centre de Neurosciences Paris-Sud, UMR 8195, F-91405 Orsay, FranceService NutritionLactalis Recherche et Développement, 8 Fromy, CS 60082, 35240 Retiers, FranceLactalis Nutrition Parc d'Activité de Torcé-secteur Est35370 Torcé, France.
authors.forename: Pascale
authors.initials: P
authors.lastname: LeRuyet
authors.affiliation: Neuroendocrinologie Moléculaire de la Prise AlimentaireUniversity of Paris-Sud, UMR 8195, F-91405 Orsay, FranceNeuroendocrinologie Moléculaire de la Prise AlimentaireCNRS, Centre de Neurosciences Paris-Sud, UMR 8195, F-91405 Orsay, FranceService NutritionLactalis Recherche et Développement, 8 Fromy, CS 60082, 35240 Retiers, FranceLactalis Nutrition Parc d'Activité de Torcé-secteur Est35370 Torcé, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Bonhomme
authors.affiliation: Neuroendocrinologie Moléculaire de la Prise AlimentaireUniversity of Paris-Sud, UMR 8195, F-91405 Orsay, FranceNeuroendocrinologie Moléculaire de la Prise AlimentaireCNRS, Centre de Neurosciences Paris-Sud, UMR 8195, F-91405 Orsay, FranceService NutritionLactalis Recherche et Développement, 8 Fromy, CS 60082, 35240 Retiers, FranceLactalis Nutrition Parc d'Activité de Torcé-secteur Est35370 Torcé, France Neuroendocrinologie Moléculaire de la Prise AlimentaireUniversity of Paris-Sud, UMR 8195, F-91405 Orsay, FranceNeuroendocrinologie Moléculaire de la Prise AlimentaireCNRS, Centre de Neurosciences Paris-Sud, UMR 8195, F-91405 Orsay, FranceService NutritionLactalis Recherche et Développement, 8 Fromy, CS 60082, 35240 Retiers, FranceLactalis Nutrition Parc d'Activité de Torcé-secteur Est35370 Torcé, France mohammed.taouis@u-psud.fr.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Taouis
mesh_terms: D000368:Aged; D000375:Aging / Q000235:genetics / Q000473:pathology / Q000502:physiology*; D000818:Animals; D004041:Dietary Fats / Q000008:administration & dosage; D019587:Dietary Supplements / Q000032:analysis; D004195:Disease Models, Animal; D004435:Eating / Q000235:genetics / Q000502:physiology*; D004734:Energy Metabolism / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D007031:Hypothalamus / Q000473:pathology / Q000503:physiopathology*; D007333:Insulin Resistance; D015850:Interleukin-6 / Q000235:genetics*; D008297:Male; D044342:Malnutrition / Q000178:diet therapy* / Q000235:genetics / Q000503:physiopathology; D008892:Milk / Q000737:chemistry; D008894:Milk Proteins / Q000008:administration & dosage; D051381:Rats; D017208:Rats, Wistar; D012995:Solubility; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics; D014807:Vitamin D / Q000008:administration & dosage; D015430:Weight Gain
chemical_list: D004041:Dietary Fats; D015850:Interleukin-6; D008894:Milk Proteins; D014409:Tumor Necrosis Factor-alpha; D014807:Vitamin D
keywords: aging; inflammation; insulin responsiveness; milk fat; milk protein