title: The Role of MicroRNA, miR-24, and Its Target CHI3L1 in Osteomyelitis Caused by Staphylococcus aureus.
abstract: Osteomyelitis is a debilitating infectious disease of the bone which is predominantly caused by Staphylococcus aureus (S. aureus). MicroRNAs (miRNAs) have been shown to play a regulatory role in osteogenesis. In the present study, the expression levels of miRNAs proposed to potentially play a regulatory role in bone formation or differentiation (miR-24, miR-29b, miR-200a, miR-208, miR-322) were analyzed in the whole blood of patients with bacterial osteomyelitis or healthy controls, and in MC3T3-E1 cells infected with S. aureus by qRT-PCR. The expression of miR-24 was significantly down-regulated in osteomyelitis patients and S. aureus-infected MC3T3-E1 cells compared with the healthy controls or untreated control cells. Moreover, our results showed that S. aureus inhibited MC3T3-E1 cell proliferation, induced osteoblast apoptosis and prohibited bone formation and mineralization. We found that overexpression of miR-24 could reduce the effects of S. aureus, while inhibition of miR-24 intensified the effects. We also demonstrated that miR-24 suppressed the expression of chitinase 3-like 1 (CHI3L1) mRNA, thought to mediate multiple signaling pathways, by directly binding to the 3'-untranslated region.
authors.affiliation: Department of Orthopedic Surgery, Kunming General Hospital Chengdu Military Command, Kunming, 650032, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Jin
authors.affiliation: Department of Radiology, School of Medicine, Ruijin Hospital, Shanghai Jiaotong University, Shanghai, 200025, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Orthopedic Surgery, Kunming General Hospital Chengdu Military Command, Kunming, 650032, PR China.
authors.forename: Qing Xiao
authors.initials: QX
authors.lastname: He
authors.affiliation: Department of Orthopedic Surgery, Kunming General Hospital Chengdu Military Command, Kunming, 650032, PR China.
authors.forename: Hai
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Orthopedic Surgery, Kunming General Hospital Chengdu Military Command, Kunming, 650032, PR China.
authors.forename: Bing Fu
authors.initials: BF
authors.lastname: Li
authors.affiliation: Department of Orthopedic Surgery, Kunming General Hospital Chengdu Military Command, Kunming, 650032, PR China.
authors.forename: Liang Yue
authors.initials: LY
authors.lastname: Zhu
authors.affiliation: Department of Orthopedic Surgery, Kunming General Hospital Chengdu Military Command, Kunming, 650032, PR China.
authors.forename: Qing Yong
authors.initials: QY
authors.lastname: Xu
mesh_terms: D054392:Adipokines / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D000328:Adult; D017209:Apoptosis / Q000235:genetics; D001842:Bone and Bones / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D002454:Cell Differentiation / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D000071451:Chitinase-3-Like Protein 1; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D037102:Lectins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D008875:Middle Aged; D010012:Osteogenesis / Q000235:genetics*; D010019:Osteomyelitis / Q000235:genetics* / Q000382:microbiology / Q000473:pathology; D011485:Protein Binding; D012333:RNA, Messenger / Q000096:biosynthesis; D015398:Signal Transduction / Q000235:genetics; D013211:Staphylococcus aureus / Q000472:pathogenicity
chemical_list: D054392:Adipokines; C084533:CHI3L1 protein, human; D000071451:Chitinase-3-Like Protein 1; D037102:Lectins; C543845:MIRN24 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger
keywords: CHI3L1; OSTEOMYELITIS; Staphylococcus aureus; miR-24; miRNA