title: Association between the TCF7L2 rs12255372 (G/T) gene polymorphism and type 2 diabetes mellitus in a Cameroonian population: a pilot study.
abstract: BACKGROUND
  To study the relationship between the rs12255372 (G/T) polymorphism of the transcription factor 7-like 2 (TCF7L2) and type 2 diabetes mellitus (T2DM) in a Cameroonian population.
  
  
  METHODS
  This case-control study included 60 T2DM patients and 60 healthy normoglycemic controls, all unrelated and of Cameroonian origin, aged above 40 years (range 40-87). The Restriction Fragment Length Polymorphism - Polymerase Chain Reaction (RFLP-PCR) was used for genotyping.
  
  
  RESULTS
  The T allele frequency was significantly higher in the diabetic group (0.44) than in the control group (0.17). This allele was significantly associated to a greater risk of developing T2DM as compared to the G allele (OR = 3.92, 95% CI 2.04 - 7.67, p < 0.0001). The codominant (additive) model explained best the risk of developing the disease, as the TT genotype was significantly associated to T2DM when compared to the GG genotype (OR = 4.45, 95% CI 1.64 - 12.83, p = 0.0014). By logistic regression adjusted for age, this OR was 4.33 (95% CI: 1.57 - 11.92, p = 0.005).
  
  
  CONCLUSIONS
  Our findings suggest that the rs12255372 (G/T) polymorphism of the TCF7L2 gene is an important risk factor for T2DM in the Cameroonian population.
authors.affiliation: Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon ; Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon.
authors.forename: Dieudonne
authors.initials: D
authors.lastname: Nanfa
authors.affiliation: Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon ; Department of Internal Medicine and Specialties, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon ; National Obesity Center, Yaoundé Central Hospital, Yaoundé, Cameroon.
authors.forename: Eugene
authors.initials: E
authors.lastname: Sobngwi
authors.affiliation: Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon ; Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon.
authors.forename: Barbara
authors.initials: B
authors.lastname: Atogho-Tiedeu
authors.affiliation: Department of Medicine, Groote Schuur Hospital and University of Cape Town, Cape Town, South Africa ; Medical Diagnostic Center, Yaoundé, Cameroon.
authors.forename: Jean Jacques N
authors.initials: JJ
authors.lastname: Noubiap
authors.affiliation: Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon ; Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon.
authors.forename: Olivier Sontsa
authors.initials: OS
authors.lastname: Donfack
authors.affiliation: Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon ; Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon.
authors.forename: Edith Pascale Mato
authors.initials: EP
authors.lastname: Mofo
authors.affiliation: Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon ; Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon.
authors.forename: Magellan
authors.initials: M
authors.lastname: Guewo-Fokeng
authors.affiliation: Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon.
authors.forename: Aurelie
authors.initials: A
authors.lastname: Nguimmo Metsadjio
authors.affiliation: Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon ; Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon.
authors.forename: Elvis
authors.initials: E
authors.lastname: Ndonwi Ngwa
authors.affiliation: Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon ; Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon.
authors.forename: Priscille
authors.initials: P
authors.lastname: Pokam Fosso
authors.affiliation: Department of Medicine, Groote Schuur Hospital and University of Cape Town, Cape Town, South Africa.
authors.forename: Eric
authors.initials: E
authors.lastname: Djahmeni
authors.affiliation: Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon ; Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon.
authors.forename: Rosine
authors.initials: R
authors.lastname: Djokam-Dadjeu
authors.affiliation: Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon ; Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon.
authors.forename: Marie-Solange
authors.initials: MS
authors.lastname: Evehe
authors.affiliation: The Canadian Pharmacogenomics Network for Drug Safety (CPNDS), Center for Molecular Medicine and Therapeutics, Department of Medical Genetics, University of British Columbia, Vancouver, Canada.
authors.forename: Folefac
authors.initials: F
authors.lastname: Aminkeng
authors.affiliation: Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon ; Laboratory for Public Health Research Biotechnologies, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon.
authors.forename: Wilfred F
authors.initials: WF
authors.lastname: Mbacham
authors.affiliation: Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon ; Department of Internal Medicine and Specialties, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon ; National Obesity Center, Yaoundé Central Hospital, Yaoundé, Cameroon.
authors.forename: Jean Claude
authors.initials: JC
authors.lastname: Mbanya
keywords: Cameroon; Genetic association; Sub-Saharan Africa; Transcription factor 7-like 2 (TCF7L2); Type 2 diabetes