title: Dietary ribonucleic acid suppresses inflammation of adipose tissue and improves glucose intolerance that is mediated by immune cells in C57BL/6 mice fed a high-fat diet.
abstract: Recent evidence suggests that immune cells play an important role in differentiation of inflammatory macrophages in adipose tissue, which contributes to systemic chronic inflammation. Dietary ribonucleic acid (RNA) has been shown to modulate immune function. We hypothesized that RNA affects immune cell function in adipose tissue and then improves inflammatory response in adipose tissue. C57/BL6 mice and recombination activating gene-1 (RAG-1) knockout mice on a C57BL/6 mice background were fed a high-fat diet containing 1% RNA for 12 wk. An oral glucose tolerance test was performed. Supplementation of dietary RNA in C57BL/6 mice fed a high-fat diet resulted in a smaller area under the curve (AUC) after oral glucose administration than that for control mice. The mRNA expression levels of inflammation-related cytokines in adipose tissue and serum interleukin-6 levels were reduced by dietary RNA supplementation. Interestingly, reduction of the AUC value by RNA supplementation was abolished in T and B cell-deficient RAG-1 knockout mice. These results indicate that RNA improves inflammation in adipose tissue and reduces the AUC value following oral glucose administration in a T and B cell-dependent manner.
authors.affiliation: Department of Public Health and Applied Nutrition, Institute of Health Biosciences, The University of Tokushima Graduate School.
authors.forename: Tohru
authors.initials: T
authors.lastname: Sakai
authors.forename: Tomoyo
authors.initials: T
authors.lastname: Taki
authors.forename: Akiko
authors.initials: A
authors.lastname: Nakamoto
authors.forename: Shiho
authors.initials: S
authors.lastname: Tazaki
authors.forename: Mai
authors.initials: M
authors.lastname: Arakawa
authors.forename: Mariko
authors.initials: M
authors.lastname: Nakamoto
authors.forename: Rie
authors.initials: R
authors.lastname: Tsutsumi
authors.forename: Emi
authors.initials: E
authors.lastname: Shuto
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D000818:Animals; D019540:Area Under Curve; D001402:B-Lymphocytes / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D016207:Cytokines / Q000097:blood / Q000235:genetics / Q000378:metabolism; D059305:Diet, High-Fat; D019587:Dietary Supplements; D018149:Glucose Intolerance / Q000178:diet therapy / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics; D007249:Inflammation / Q000178:diet therapy* / Q000209:etiology; D015850:Interleukin-6 / Q000097:blood; D008214:Lymphocytes / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism; D008297:Male; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009765:Obesity / Q000209:etiology / Q000378:metabolism / Q000473:pathology*; D012313:RNA / Q000494:pharmacology / Q000627:therapeutic use*; D012333:RNA, Messenger / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D016207:Cytokines; D018398:Homeodomain Proteins; D015850:Interleukin-6; D012333:RNA, Messenger; C064658:RAG-1 protein; D012313:RNA