title: Attenuation of radiation-induced gastrointestinal damage by epidermal growth factor and bone marrow transplantation in mice.
abstract: OBJECTIVE
  We examined the effect of epidermal growth factor (EGF) and bone marrow transplantation (BMT) on gastrointestinal damage after high-dose irradiation of mice.
  
  
  METHODS
  C57Black/6 mice were used. Two survival experiments were performed (12 and 13 Gy; (60)Co, 0.59-0.57 Gy/min). To evaluate BMT and EGF action, five groups were established - 0 Gy, 13 Gy, 13 Gy + EGF (at 2 mg/kg, first dose 24 h after irradiation and then every 48 h), 13 Gy + BMT (5 × 10(6) cells from green fluorescent protein [GFP] syngenic mice, 4 h after irradiation), and 13 Gy + BMT + EGF. Survival data, blood cell counts, gastrointestine and liver parameters and GFP positive cell migration were measured.
  
  
  RESULTS
  BMT and EGF (three doses, at 2 mg/kg, administered 1, 3 and 5 days after irradiation) significantly increased survival (13 Gy). In blood, progressive cytopenia was observed with BMT, EGF or their combination having no improving effect early after irradiation. In gastrointestinal system, BMT, EGF and their combination attenuated radiation-induced atrophy and increased regeneration during first week after irradiation with the combination being most effective. Signs of systemic inflammatory reaction were observed 30 days after irradiation.
  
  
  CONCLUSIONS
  Our data indicate that BMT together with EGF is a promising strategy in the treatment of high-dose whole-body irradiation damage.
authors.affiliation: a Department of Radiobiology , Faculty of Military Health Sciences, University of Defence , Trebesska, Hradec Kralove , Czech Republic.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Pejchal
authors.affiliation: a Department of Radiobiology , Faculty of Military Health Sciences, University of Defence , Trebesska, Hradec Kralove , Czech Republic.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Šinkorová
authors.affiliation: a Department of Radiobiology , Faculty of Military Health Sciences, University of Defence , Trebesska, Hradec Kralove , Czech Republic.
authors.forename: Aleš
authors.initials: A
authors.lastname: Tichý
authors.affiliation: a Department of Radiobiology , Faculty of Military Health Sciences, University of Defence , Trebesska, Hradec Kralove , Czech Republic.
authors.forename: Adéla
authors.initials: A
authors.lastname: Kmochová
authors.affiliation: a Department of Radiobiology , Faculty of Military Health Sciences, University of Defence , Trebesska, Hradec Kralove , Czech Republic.
authors.forename: Kamila
authors.initials: K
authors.lastname: Ďurišová
authors.affiliation: b Department of Molecular Pathology and Biology , Faculty of Military Health Sciences, University of Defence , Trebesska, Hradec Kralove , Czech Republic.
authors.forename: Klára
authors.initials: K
authors.lastname: Kubelková
authors.affiliation: b Department of Molecular Pathology and Biology , Faculty of Military Health Sciences, University of Defence , Trebesska, Hradec Kralove , Czech Republic.
authors.forename: Miroslav
authors.initials: M
authors.lastname: Pohanka
authors.affiliation: c 2nd Department of Internal Medicine - Gastroenterology , Faculty of Medicine in Hradec Kralove, Charles University in Prague , Simkova, Hradec Kralove , Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Bureš
authors.affiliation: c 2nd Department of Internal Medicine - Gastroenterology , Faculty of Medicine in Hradec Kralove, Charles University in Prague , Simkova, Hradec Kralove , Czech Republic.
authors.forename: Ilja
authors.initials: I
authors.lastname: Tachecí
authors.affiliation: d Biomedical Reseach Centre, University Hospital , Sokolska, Hradec Kralove , Czech Republic.
authors.forename: Kamil
authors.initials: K
authors.lastname: Kuča
authors.affiliation: a Department of Radiobiology , Faculty of Military Health Sciences, University of Defence , Trebesska, Hradec Kralove , Czech Republic.
authors.forename: Jiřina
authors.initials: J
authors.lastname: Vávrová
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D016026:Bone Marrow Transplantation; D003131:Combined Modality Therapy; D004815:Epidermal Growth Factor / Q000008:administration & dosage / Q000627:therapeutic use*; D005260:Female; D041981:Gastrointestinal Tract / Q000293:injuries* / Q000473:pathology / Q000528:radiation effects*; D007249:Inflammation / Q000473:pathology; D051847:Lithostathine / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008938:Mitosis / Q000187:drug effects / Q000528:radiation effects; D011833:Radiation Injuries, Experimental / Q000188:drug therapy / Q000473:pathology / Q000628:therapy*; D014916:Whole-Body Irradiation / Q000009:adverse effects
chemical_list: D051847:Lithostathine; D004815:Epidermal Growth Factor
keywords: Ionizing radiation; acute radiation syndrome; bone marrow transplantation; epidermal growth factor; green fluorescent protein; mice