title: MicroRNAs 9 and 370 Association with Biochemical Markers in T2D and CAD Complication of T2D.
abstract: BACKGROUND
  MicroRNAs (miRNAs) are small non coding RNAs with essential roles, of which any alteration leads to several conditions. Their roles in diabetes (DM) and its vascular complications have not been completely assessed.
  
  
  OBJECTIVE
  to study the association of two miRNAs; 9 and 370, with biochemical parameters of type 2 diabetic (T2D), dyslipidemia and coronary artery disease (CAD).
  
  
  METHODS
  Blood samples were taken from 200 subjects of both genders, in the Outpatient clinic of Al Qasr El-Einy teaching hospitals, in which levels of both miRNAs (using real time PCR) and routine parameters were measured. Subjects were divided over four groups, 50 in each group as follows; patients with T2D, patients with CAD, patients with T2D and CAD, and healthy control subjects.
  
  
  RESULTS
  miRNA 9 levels were expected to be over expressed in diabetic patients, while miRNA 370 levels were expected to be over expressed in those suffering from CAD and their association with CAD complication of T2D.
  
  
  RESULTS
  miRNA 9 levels were significantly higher in T2D patients and T2D patients with CAD, (1.18±0.07, and 1.31±0.08 respectively), while miRNA 370 levels were significantly higher in T2D patients, CAD patients, and T2D patients with CAD (0.59±0.05, 1.00±0.05, and 1.20±0.06 respectively), compared to control group at p = 0.000. In addition both miRNAs were still significantly associated with each other even after conducting multiple regression analysis.
  
  
  CONCLUSIONS
  This study associates the possible role of miRNAs in the diagnosis/prognosis of CAD complication of T2D.
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
authors.forename: Tarek M
authors.initials: TM
authors.lastname: Motawae
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
authors.forename: Manal F
authors.initials: MF
authors.lastname: Ismail
authors.affiliation: Department of Biochemistry, Faculty of Pharmaceutical Sciences and Pharmaceutical Industries, Future University, Cairo, Egypt.
authors.forename: Marwa I
authors.initials: MI
authors.lastname: Shabayek
authors.affiliation: Department of Biochemistry, Faculty of Pharmaceutical Sciences and Pharmaceutical Industries, Future University, Cairo, Egypt.
authors.forename: Mae M
authors.initials: MM
authors.lastname: Seleem
mesh_terms: D000368:Aged; D015415:Biomarkers; D016022:Case-Control Studies; D003324:Coronary Artery Disease / Q000209:etiology / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000235:genetics*; D003925:Diabetic Angiopathies / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics / Q000502:physiology*; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D015415:Biomarkers; C526503:MIRN370 microRNA, human; C541874:MIRN92 microRNA, human; D035683:MicroRNAs