title: An Integrated Metagenomics/Metaproteomics Investigation of the Microbial Communities and Enzymes in Solid-state Fermentation of Pu-erh tea.
abstract: Microbial enzymes during solid-state fermentation (SSF), which play important roles in the food, chemical, pharmaceutical and environmental fields, remain relatively unknown. In this work, the microbial communities and enzymes in SSF of Pu-erh tea, a well-known traditional Chinese tea, were investigated by integrated metagenomics/metaproteomics approach. The dominant bacteria and fungi were identified as Proteobacteria (48.42%) and Aspergillus (94.98%), through pyrosequencing-based analyses of the bacterial 16S and fungal 18S rRNA genes, respectively. In total, 335 proteins with at least two unique peptides were identified and classified into 28 Biological Processes and 35 Molecular Function categories using a metaproteomics analysis. The integration of metagenomics and metaproteomics data demonstrated that Aspergillus was dominant fungus and major host of identified proteins (50.45%). Enzymes involved in the degradation of the plant cell wall were identified and associated with the soft-rotting of tea leaves. Peroxiredoxins, catalase and peroxidases were associated with the oxidation of catechins. In conclusion, this work greatly advances our understanding of the SSF of Pu-erh tea and provides a powerful tool for studying SSF mechanisms, especially in relation to the microbial communities present.
authors.affiliation: 1] College of Longrun Pu-erh Tea, Yunnan Agricultural University, Kunming 650201, Yunnan, China [2] Yunnan Research Center on Good Agricultural Practice for Dominant Chinese Medicinal Materials, Yunnan Agricultural University, Kunming 650201, Yunnan, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhao
authors.affiliation: College of Longrun Pu-erh Tea, Yunnan Agricultural University, Kunming 650201, Yunnan, China.
authors.forename: Dong-Lian
authors.initials: DL
authors.lastname: Zhang
authors.affiliation: College of Longrun Pu-erh Tea, Yunnan Agricultural University, Kunming 650201, Yunnan, China.
authors.forename: Xiao-Qin
authors.initials: XQ
authors.lastname: Su
authors.affiliation: College of Longrun Pu-erh Tea, Yunnan Agricultural University, Kunming 650201, Yunnan, China.
authors.forename: Shuang-Mei
authors.initials: SM
authors.lastname: Duan
authors.affiliation: College of Longrun Pu-erh Tea, Yunnan Agricultural University, Kunming 650201, Yunnan, China.
authors.forename: Jin-Qiong
authors.initials: JQ
authors.lastname: Wan
authors.affiliation: College of Longrun Pu-erh Tea, Yunnan Agricultural University, Kunming 650201, Yunnan, China.
authors.forename: Wen-Xia
authors.initials: WX
authors.lastname: Yuan
authors.affiliation: Tea Research Institute of Yunnan Academy of Agricultural Science, Menghai 666201, China.
authors.forename: Ben-Ying
authors.initials: BY
authors.lastname: Liu
authors.affiliation: College of Longrun Pu-erh Tea, Yunnan Agricultural University, Kunming 650201, Yunnan, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Ying-Hong
authors.initials: YH
authors.lastname: Pan
mesh_terms: D001230:Aspergillus / Q000235:genetics* / Q000378:metabolism; D028241:Camellia sinensis / Q000378:metabolism / Q000382:microbiology*; D002374:Catalase / Q000378:metabolism; D005285:Fermentation / Q000502:physiology; D056186:Metagenomics; D059013:Microbial Consortia / Q000235:genetics; D010544:Peroxidases / Q000378:metabolism; D054464:Peroxiredoxins / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism / Q000382:microbiology; D020560:Proteobacteria / Q000235:genetics* / Q000378:metabolism; D040901:Proteomics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D060327:Solid-Phase Synthesis Techniques / Q000379:methods*; D013662:Tea / Q000378:metabolism* / Q000382:microbiology
chemical_list: D012336:RNA, Ribosomal, 16S; D012337:RNA, Ribosomal, 18S; D013662:Tea; D010544:Peroxidases; D054464:Peroxiredoxins; D002374:Catalase