title: Transcriptional responses of earthworm (Eisenia fetida) exposed to naphthenic acids in soil.
abstract: In this study, earthworms (Eisenia fetida) were exposed to commercial NAs contaminated soil, and changes in the levels of reactive oxygen species (ROS) and gene expressions of their defense system were monitored. The effects on the gene expression involved in reproduction and carcinogenesis were also evaluated. Significant increases in ROS levels was observed in NAs exposure groups, and the superoxide dismutase (SOD) and catalase (CAT) genes were both up-regulated at low and medium exposure doses, which implied NAs might exert toxicity by oxidative stress. The transcription of CRT and HSP70 coincided with oxidative stress, which implied both chaperones perform important functions in the protection against oxidative toxicity. The upregulation of TCTP gene indicated a potential adverse effect of NAs to terrestrial organisms through induction of carcinogenesis, and the downregulation of ANN gene indicated that NAs might potentially result in deleterious reproduction effects.
authors.affiliation: State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Cao
authors.affiliation: Chinese Research Academy of Environmental Sciences, Beijing 100012, China.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Sun
authors.affiliation: State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China.
authors.forename: Liwei
authors.initials: L
authors.lastname: Chai
authors.affiliation: State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China. Electronic address: yhuang@pku.edu.cn.
authors.forename: Yi
authors.initials: Y
authors.lastname: Huang
authors.affiliation: State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Tang
mesh_terms: D000818:Animals; D002264:Carboxylic Acids / Q000633:toxicity*; D002374:Catalase / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D009835:Oligochaeta / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D012989:Soil Pollutants / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000235:genetics; D015854:Up-Regulation
chemical_list: D002264:Carboxylic Acids; D018840:HSP70 Heat-Shock Proteins; D017382:Reactive Oxygen Species; D012989:Soil Pollutants; D002374:Catalase; D013482:Superoxide Dismutase; C046170:naphthenic acid
keywords: Ecotoxicity; Eisenia fetida; Naphthenic acids; Quantitative real-time PCR; ROS