title: Decorin in Human Colon Cancer: Localization In Vivo and Effect on Cancer Cell Behavior In Vitro.
abstract: Decorin is generally recognized as a tumor suppressing molecule. Nevertheless, although decorin has been shown to be differentially expressed in malignant tissues, it has often remained unclear whether, in addition to non-malignant stromal cells, cancer cells also express it. Here, we first used two publicly available databases to analyze the current information about decorin expression and immunoreactivity in normal and malignant human colorectal tissue samples. The analyses demonstrated that decorin expression and immunoreactivity may vary in cancer cells of human colorectal tissues. Therefore, we next examined decorin expression in normal, premalignant and malignant human colorectal tissues in more detail using both in situ hybridization and immunohistochemistry for decorin. Our results invariably demonstrate that malignant cells within human colorectal cancer tissues are devoid of both decorin mRNA and immunoreactivity. Identical results were obtained for cells of neuroendocrine tumors of human colon. Using RT-qPCR, we showed that human colon cancer cell lines are also decorin negative, in accordance with the above in vivo results. Finally, we demonstrate that decorin transduction of human colon cancer cell lines causes a significant reduction in their colony forming capability. Thus, strategies to develop decorin-based adjuvant therapies for human colorectal malignancies are highly rational.
authors.affiliation: Department of Medical Biochemistry and Genetics,  University of Turku, Turku, Finland (MCN, AOS, MMP, HTJ)
authors.forename: Marie C
authors.initials: MC
authors.lastname: Nyman
authors.affiliation: Department of Medical Biochemistry and Genetics,  University of Turku, Turku, Finland (MCN, AOS, MMP, HTJ)
authors.forename: Annele O
authors.initials: AO
authors.lastname: Sainio
authors.affiliation: Department of Medical Biochemistry and Genetics,  University of Turku, Turku, Finland (MCN, AOS, MMP, HTJ)
authors.forename: Mirka M
authors.initials: MM
authors.lastname: Pennanen
authors.affiliation: Turku Centre for Biotechnology , University of Turku, Turku, Finland(RJL, SV)
authors.forename: Riikka J
authors.initials: RJ
authors.lastname: Lund
authors.affiliation: Turku Centre for Biotechnology , University of Turku, Turku, Finland(RJL, SV)
authors.forename: Sanna
authors.initials: S
authors.lastname: Vuorikoski
authors.affiliation: Department of Pathology , University of Turku, Turku, Finland(JTTS)
authors.forename: Jari T T
authors.initials: JT
authors.lastname: Sundström
authors.affiliation: Division of Medicine, Department of Endocrinology, Turku University Hospital, Turku, Finland (HTJ)
authors.forename: Hannu T
authors.initials: HT
authors.lastname: Järveläinen
mesh_terms: D063646:Carcinogenesis; D002277:Carcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D003106:Colon / Q000166:cytology / Q000473:pathology; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D019175:DNA Methylation; D030562:Databases, Protein; D058575:Decorin / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D018358:Neuroendocrine Tumors / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D011401:Promoter Regions, Genetic / Q000235:genetics; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: C546878:DCN protein, human; D058575:Decorin; D012333:RNA, Messenger
keywords: adenoviral transduction; antitumorigenic; colon cancer; decorin; neuroendocrine tumor