title: Adipose-Derived Mesenchymal Stem Cells (ADSCs) With the Potential to Ameliorate Platelet Recovery, Enhance Megakaryopoiesis, and Inhibit Apoptosis of Bone Marrow Cells in a Mouse Model of Radiation-Induced Thrombocytopenia.
abstract: Substantial damage to the bone marrow can be caused by exposure to radiation, which can then develop into severe thrombocytopenia. In this study, we investigated the in vivo impact of adipose-derived mesenchymal stem cells (ADSCs) on megakaryopoiesis and platelet recovery in irradiated mice. Radiation markedly reduced peripheral blood counts. Recovery of both platelets and WBCs was better in the ADSC-treated group compared with the saline group and the fibroblast group 21 days after irradiation. A significant increase in the total CFU and MK-CFU after irradiation was observed in the ADSC group compared with the saline group and the fibroblast group. Further, the proportion of CD41(+) cells in the ADSC group was significantly higher than that in the saline group and the fibroblast group. ADSC treatment significantly improved the cellularity and decreased the apoptotic cells in the bone marrow while normal fibroblasts did not. Administration of ADSCs upregulated protein expression of phosphorylated Akt and Bcl-xL, whereas the expression of Bax, a protein related to apoptosis, was significantly lower in the ADSC group. In conclusion, this study suggests that ADSCs were capable of promoting platelet recovery, improving megakaryopoiesis, and inhibiting apoptosis of bone marrow cells in irradiated mice. The antiapoptotic effect of ADSCs is likely to be mediated via the PI3K/Akt pathway. These findings may provide a scientific basis for using ADSCs as a new therapy after irradiation.
authors.affiliation: Peking University People's Hospital, Peking University Institute of Hematology, Beijing, China.
authors.forename: Jiamin
authors.initials: J
authors.lastname: Zhang
authors.forename: Shiyuan
authors.initials: S
authors.lastname: Zhou
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhou
authors.forename: Feier
authors.initials: F
authors.lastname: Feng
authors.forename: Qianming
authors.initials: Q
authors.lastname: Wang
authors.forename: Xiaolu
authors.initials: X
authors.lastname: Zhu
authors.forename: Jingzhong
authors.initials: J
authors.lastname: Zhao
authors.forename: Haixia
authors.initials: H
authors.lastname: Fu
authors.forename: Meng
authors.initials: M
authors.lastname: Lv
authors.forename: Huisheng
authors.initials: H
authors.lastname: Ai
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Huang
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001792:Blood Platelets / Q000166:cytology*; D001854:Bone Marrow Cells / Q000166:cytology*; D049109:Cell Proliferation / Q000502:physiology; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005347:Fibroblasts / Q000166:cytology; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008807:Mice, Inbred BALB C; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D013921:Thrombocytopenia / Q000276:immunology*
chemical_list: D019869:Phosphatidylinositol 3-Kinases