title: Effect of MTHFR, TGFβ1, and TNFB polymorphisms on osteoporosis in rheumatoid arthritis patients.
abstract: Diseases of the immune and the skeletal systems should be studied together for the deep interaction between them. Many studies consider osteoporosis (OP) as a risk factor for the prediction of disease progression in rheumatoid arthritis (RA). The aim of this research is to study the effect of four single nucleotide polymorphisms (SNPs) on RA patients with and without OP. The examined SNPs (MTHFR (C677T, and A1298C), TGFβ1 (T869C), and TNFB (A252G)) were tested by genotyping 17 RA patients with OP and 72 RA patients without OP. Associations were tested using four models (multiplicative, dominant, recessive, and co-dominant). The studied SNPs were not significantly associated with the risk of OP in RA. MTHFR, TGFβ1, and TNFB polymorphisms don't appear to be clinically useful genetic markers for predicting RA severity in Egyptian women population.
authors.affiliation: Biomedical Engineering Department, Minia University, Minia, Egypt. Electronic address: m.n.saad@ieee.org.
authors.forename: Mohamed N
authors.initials: MN
authors.lastname: Saad
authors.affiliation: Biomedical Engineering Department, MUST, 6th of October, Egypt.
authors.forename: Mai S
authors.initials: MS
authors.lastname: Mabrouk
authors.affiliation: Systems and Biomedical Engineering Department, Cairo University, Giza, Egypt.
authors.forename: Ayman M
authors.initials: AM
authors.lastname: Eldeib
authors.affiliation: Medical Biochemistry and Molecular Biology Department, Cairo University, Cairo, Egypt.
authors.forename: Olfat G
authors.initials: OG
authors.lastname: Shaker
mesh_terms: D000328:Adult; D001172:Arthritis, Rheumatoid / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D008233:Lymphotoxin-alpha / Q000235:genetics*; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D008875:Middle Aged; D010024:Osteoporosis / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D053773:Transforming Growth Factor beta1 / Q000235:genetics*
chemical_list: D008233:Lymphotoxin-alpha; C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1; C573423:MTHFR protein, human; D042965:Methylenetetrahydrofolate Reductase (NADPH2)
keywords: Egyptian population; MTHFR; Osteoporosis; Rheumatoid arthritis; Single nucleotide polymorphism; TGFβ1; TNFB