title: One-Step Formation of "Chain-Armor"-Stabilized DNA Nanostructures.
abstract: DNA-based self-assembled nanostructures are widely used to position organic and inorganic objects with nanoscale precision. A particular promising application of DNA structures is their usage as programmable carrier systems for targeted drug delivery. To provide DNA-based templates that are robust against degradation at elevated temperatures, low ion concentrations, adverse pH conditions, and DNases, we built 6-helix DNA tile tubes consisting of 24 oligonucleotides carrying alkyne groups on their 3'-ends and azides on their 5'-ends. By a mild click reaction, the two ends of selected oligonucleotides were covalently connected to form rings and interlocked DNA single strands, so-called DNA catenanes. Strikingly, the structures stayed topologically intact in pure water and even after precipitation from EtOH. The structures even withstood a temperature of 95 °C when all of the 24 strands were chemically interlocked.
authors.affiliation: baseclick GmbH, Bahnhofstrasse 9-15, 82327 Tutzing (Germany).|Department Chemistry and Biochemistry, Ludwig-Maximilians-Universität (LMU), Butenandtstrasse 5-13, 81377 Munich (Germany).
authors.forename: Valentina
authors.initials: V
authors.lastname: Cassinelli
authors.affiliation: baseclick GmbH, Bahnhofstrasse 9-15, 82327 Tutzing (Germany).
authors.forename: Birgit
authors.initials: B
authors.lastname: Oberleitner
authors.affiliation: baseclick GmbH, Bahnhofstrasse 9-15, 82327 Tutzing (Germany).|Department Applied Chemistry, Technische Hochschule Nürnberg G. S. Ohm, Kesslerplatz 12, 90489 Nürnberg (Germany).
authors.forename: Jessica
authors.initials: J
authors.lastname: Sobotta
authors.affiliation: Physics Department and CeNS, Ludwig-Maximilians-Universität (LMU), Geschwister-Scholl-Platz 1, 80539 Munich (Germany).
authors.forename: Philipp
authors.initials: P
authors.lastname: Nickels
authors.affiliation: Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus (Denmark).
authors.forename: Guido
authors.initials: G
authors.lastname: Grossi
authors.affiliation: Physics Department and CeNS, Ludwig-Maximilians-Universität (LMU), Geschwister-Scholl-Platz 1, 80539 Munich (Germany).
authors.forename: Susanne
authors.initials: S
authors.lastname: Kempter
authors.affiliation: baseclick GmbH, Bahnhofstrasse 9-15, 82327 Tutzing (Germany).
authors.forename: Thomas
authors.initials: T
authors.lastname: Frischmuth
authors.affiliation: Physics Department and CeNS, Ludwig-Maximilians-Universität (LMU), Geschwister-Scholl-Platz 1, 80539 Munich (Germany).
authors.forename: Tim
authors.initials: T
authors.lastname: Liedl
authors.affiliation: baseclick GmbH, Bahnhofstrasse 9-15, 82327 Tutzing (Germany). a.manetto@baseclick.eu.
authors.forename: Antonio
authors.initials: A
authors.lastname: Manetto
mesh_terms: D000480:Alkynes / Q000737:chemistry*; D001386:Azides / Q000737:chemistry*; D057930:Click Chemistry; D004247:DNA / Q000737:chemistry*; D043864:DNA, Catenated / Q000737:chemistry; D006358:Hot Temperature; D036103:Nanotechnology; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D009841:Oligonucleotides / Q000737:chemistry
chemical_list: D000480:Alkynes; D001386:Azides; D043864:DNA, Catenated; D009841:Oligonucleotides; D004247:DNA
keywords: DNA catenanes; DNA nanotechnology; DNA tiles; click chemistry; stability