title: Chemolithoautotrophic arsenite oxidation by a thermophilic Anoxybacillus flavithermus strain TCC9-4 from a hot spring in Tengchong of Yunnan, China.
abstract: A new facultative chemolithoautotrophic arsenite (As(III))-oxidizing bacterium TCC9-4 was isolated from a hot spring microbial mat in Tengchong of Yunnan, China. This strain could grow with As(III) as an energy source, CO2-HCO3 (-) as a carbon source and oxygen as the electron acceptor in a minimal salts medium. Under chemolithoautotrophic conditions, more than 90% of 100 mg/L As(III) could be oxidized by the strain TCC9-4 in 36 h. Temperature was an important environmental factor that strongly influenced the As(III) oxidation rate and As(III) oxidase (Aio) activity; the highest Aio activity was found at the temperature of 40∘C. Addition of 0.01% yeast extract enhanced the growth significantly, but delayed the As(III) oxidation. On the basis of 16S rRNA phylogenetic sequence analysis, strain TCC9-4 was identified as Anoxybacillus flavithermus. To our best knowledge, this is the first report of arsenic (As) oxidation by A. flavithermus. The Aio gene in TCC9-4 might be quite novel relative to currently known gene sequences. The results of this study expand our current understanding of microbially mediated As oxidation in hot springs.
authors.affiliation: State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences Wuhan, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences Wuhan, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.affiliation: State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences Wuhan, China ; School of Environmental Studies, China University of Geosciences Wuhan, China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences Wuhan, China.
authors.forename: Xinyue
authors.initials: X
authors.lastname: Dai
authors.affiliation: State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences Wuhan, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences Wuhan, China ; School of Environmental Studies, China University of Geosciences Wuhan, China.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences Wuhan, China ; School of Environmental Studies, China University of Geosciences Wuhan, China.
authors.forename: Qinghai
authors.initials: Q
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences Wuhan, China ; School of Environmental Studies, China University of Geosciences Wuhan, China.
authors.forename: Yanxin
authors.initials: Y
authors.lastname: Wang
keywords: Anoxybacillus; Tengchong; arsenite oxidation; hot springs; temperature