title: Production of metallo-β-lactamase among Pseudomonas aeruginosa strains isolated in the State of Sergipe, Brazil.
abstract: BACKGROUND
  Acquired production of metallo-β-lactamases is an important mechanism of resistance in Pseudomonas aeruginosa. The objective of this study was to investigate the production of metallo-β-lactamase and the genetic diversity among ceftazidime-resistant P. aeruginosa isolates from State of Sergipe, Brazil.
  
  
  METHODS
  Metallo-β-lactamase was investigated using the disk approximation test and polymerase chain reaction (PCR). Genetic diversity was evaluated by pulsed-field gel electrophoresis (PFGE).
  
  
  RESULTS
  A total of 48 (51.6%) isolates were resistant to ceftazidime. Six (12.2%) of these were positive for metallo-β-lactamase production. Only two (4.1%) of the ceftazidime-resistant isolates carried the bla SPM-1 gene.
  
  
  CONCLUSIONS
  Production of metallo-β-lactamases was not the main mechanism of resistance to ceftazidime and carbapenems among P. aeruginosa strains in Sergipe, Brazil.
authors.affiliation: Pós-Graduação em Patologia, Departamento de Patologia, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil.
authors.forename: Lívia Maria do Amorim
authors.initials: LM
authors.lastname: Costa
authors.affiliation: Departamento de Tecnologia Farmacêutica e Cosméticos, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil.
authors.forename: Maria Emília de Castro Kling
authors.initials: ME
authors.lastname: Fleming
authors.affiliation: Departamento de Tecnologia Farmacêutica e Cosméticos, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil.
authors.forename: Geraldo Renato de
authors.initials: GR
authors.lastname: Paula
authors.affiliation: Departamento de Tecnologia Farmacêutica e Cosméticos, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil.
authors.forename: Lenise Arneiro
authors.initials: LA
authors.lastname: Teixeira
authors.affiliation: Pós-Graduação em Patologia, Departamento de Patologia, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil.
authors.forename: Pedro Juan José
authors.initials: PJ
authors.lastname: Mondino
authors.affiliation: Pós-Graduação em Patologia, Departamento de Patologia, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil.
authors.forename: Sílvia Susana Bona
authors.initials: SS
authors.lastname: de Mondino
authors.affiliation: Pós-Graduação em Patologia, Departamento de Patologia, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil.
authors.forename: Cláudia Rezende de Vieira
authors.initials: CR
authors.lastname: Mendonça-Souza
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001938:Brazil; D052978:Disk Diffusion Antimicrobial Tests; D024881:Drug Resistance, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D014644:Genetic Variation; D006801:Humans; D016133:Polymerase Chain Reaction; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D001618:beta-Lactamases / Q000096:biosynthesis*
chemical_list: D000900:Anti-Bacterial Agents; D001618:beta-Lactamases