title: Detection of glutathione within single erythrocyte of different ages and pathological state using microfluidic chips coupled with laser induced fluorescence.
abstract: As a major factor participating in the organism antioxidation and detoxification process, GSH is of vital importance to human beings. Detecting GSH content in single cells is significant to diagnosis and prevention of many diseases. In this work, the amount of GSH within single erythrocytes was detected and analyzed via statistical analysis. All erythrocytes tested were collected from people in different ages and people of different pathological states. The correlation between GSH level, age and pathological state were investigated. Results showed that the GSH level in erythrocytes decreased with the ages of patients increased. There was little difference between the GSH level in erythrocytes from people who had chronic diseases (hyperglycemia, hyperlipidemia and hypertension) and from healthy people. However, the GSH level in erythrocytes from people who had inflammation (myocarditis, nephritis and gastritis) was generally higher than that from the healthy people. This study provides basic data for researches of cell senescence and cytopathic effect and is helpful to diagnosis and prevention of diseases. In addition, it also provides a simple and effective method for rapid GSH detection within single cell.
authors.affiliation: School of Environmental Science and Engineering, China-America CRC for Environment & Health, Shandong Province, Shandong University, 27# Shanda South Road, Jinan 250100, PR China.
authors.forename: Minglu
authors.initials: M
authors.lastname: Hao
authors.affiliation: School Hospital of Shandong University, 91# Shanda North Road, Jinan 250100, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.affiliation: School of Environmental Science and Engineering, China-America CRC for Environment & Health, Shandong Province, Shandong University, 27# Shanda South Road, Jinan 250100, PR China. Electronic address: rutaoliu@sdu.edu.cn.
authors.forename: Rutao
authors.initials: R
authors.lastname: Liu
authors.affiliation: School of Environmental Science and Engineering, China-America CRC for Environment & Health, Shandong Province, Shandong University, 27# Shanda South Road, Jinan 250100, PR China.
authors.forename: Mingyang
authors.initials: M
authors.lastname: Jing
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism*; D002138:Calibration; D002452:Cell Count; D002908:Chronic Disease; D004912:Erythrocytes / Q000378:metabolism* / Q000473:pathology*; D005453:Fluorescence; D005978:Glutathione / Q000032:analysis*; D006801:Humans; D007249:Inflammation / Q000473:pathology; D007834:Lasers; D044085:Microfluidics / Q000379:methods*; D008875:Middle Aged; D055815:Young Adult
chemical_list: D005978:Glutathione
keywords: GSH detection; Laser induced fluorescence; Pathological state; Single cell