title: [Uncertainty evaluation for determining ginsenosides in ginseng food by HPLC].
abstract: OBJECTIVE
  To discuss the influencing factors for uncertainty and evaluate the measurement uncertainty for the determination of ginsenosides Rg1, Re, Rb1 and Rd in ginseng food by HPLC.
  
  
  METHODS
  According to the regulation of JJF1059. 1 - 2012, the components of uncertainty in the measuring process were studied and quantified for the calculation of combined uncertainties and expanded uncertainties.
  
  
  RESULTS
  The expanded uncertainties for the HPLC determination of ginsenosides Rg1, Re, Rb1 and Rd were 0. 0766, 0. 0700, 0. 0628 and 0. 0722 g/100g (k = 2) respectively from sample of Radix ginseng, and expanded uncertainties of ginsenosides Rg1, Re, Rb1 and Rd were 0. 0476, 0. 0672, 0. 0516 and 0. 0514 g/100g(k = 2) from sample of Red ginseng.
  
  
  CONCLUSIONS
  The uncertainty from pretreatment and standard curve fitting process were the main sources of uncertainty.
authors.forename: Shi
authors.initials: S
authors.lastname: Shen
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Ye
authors.initials: Y
authors.lastname: Bi
authors.forename: Zhu
authors.initials: Z
authors.lastname: Wang
mesh_terms: D002491:Central Nervous System Agents; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D036145:Ginsenosides / Q000032:analysis*; D005894:Panax / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D035501:Uncertainty
chemical_list: D002491:Central Nervous System Agents; D036145:Ginsenosides; C049864:ginsenoside Re; C442759:ginsenoside Rb1; C035054:ginsenoside Rg1