title: Diversity of Intestinal Clostridium coccoides Group in the Japanese Population, as Demonstrated by Reverse Transcription-Quantitative PCR.
abstract: We used sensitive rRNA-targeted reverse transcription-quantitative PCR (RT-qPCR) to quantify the Clostridium coccoides group, which is a major anaerobic population in the human intestine. For this purpose, the C. coccoides group was classified into 3 subgroups and 19 species for expediency in accordance with the existing database, and specific primers were newly developed to evaluate them. Population levels of the C. coccoides group in human feces determined by RT-qPCR were equivalent to those determined by fluorescence in situ hybridization. RT-qPCR analysis of fecal samples from 96 volunteers (32 young children, 32 adults and 32 elderly) by using the 22 new primer sets together with the C. coccoides group-specific primer setm revealed that (i) total counts obtained as the sum of the 3 subgroups and 19 species were equivalent to the results obtained by using the C. coccoides group-specific primer set; (ii) total C. coccoides-group counts in the elderly were significantly lower than those in young children and adults; (iii) genus Blautia was the most common subgroup in the human intestinal C. coccoides-group populations at all age populations tested; (iv) the prevalences of Fusicatenibacter saccharivorans and genus Dorea were significantly higher in adults than in young children and the elderly; and (v) the prevalences of C. scindens and C. hylemonae, both of which produce secondary bile acid in the human intestine, were significantly higher in the elderly than in young children and adults. Hierarchical clustering and principal component analysis showed clear separation of the bacterial components between adult and elderly populations. Taken together, these data suggest that aging plays an important role in the diversity of C. coccoides-group populations in human intestinal microbiota; changes in this diversity likely influence the health of the host.
authors.affiliation: Yakult Central Institute, 5-11 Izumi, Kunitachi, Tokyo, 186-8650, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kurakawa
authors.affiliation: Yakult Central Institute, 5-11 Izumi, Kunitachi, Tokyo, 186-8650, Japan.
authors.forename: Kiyohito
authors.initials: K
authors.lastname: Ogata
authors.affiliation: Yakult Central Institute, 5-11 Izumi, Kunitachi, Tokyo, 186-8650, Japan; Yakult Honsha European Research Center for Microbiology, ESV, Technologiepark 4, Gent-Zwijnaarde, 9052, Belgium.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Matsuda
authors.affiliation: Yakult Central Institute, 5-11 Izumi, Kunitachi, Tokyo, 186-8650, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Tsuji
authors.affiliation: Yakult Central Institute, 5-11 Izumi, Kunitachi, Tokyo, 186-8650, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kubota
authors.affiliation: Yakult Central Institute, 5-11 Izumi, Kunitachi, Tokyo, 186-8650, Japan.
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Takada
authors.affiliation: Yakult Central Institute, 5-11 Izumi, Kunitachi, Tokyo, 186-8650, Japan.
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Kado
authors.affiliation: Yakult Central Institute, 5-11 Izumi, Kunitachi, Tokyo, 186-8650, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Asahara
authors.affiliation: Yakult Central Institute, 5-11 Izumi, Kunitachi, Tokyo, 186-8650, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Takahashi
authors.affiliation: Yakult Central Institute, 5-11 Izumi, Kunitachi, Tokyo, 186-8650, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Nomoto
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002675:Child, Preschool; D003013:Clostridium / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D005243:Feces / Q000382:microbiology; D005260:Female; D014644:Genetic Variation; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D007564:Japan; D008297:Male; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction