title: Sagunja-Tang Improves Lipid Related Disease in a Postmenopausal Rat Model and HepG2 Cells.
abstract: The present study was conducted to investigate the effect of Sagunja-tang on the lipid related disease in a rat model of menopausal hyperlipidemia and lipid accumulation in methyl-β-cyclodextrin-induced HepG2 cells. In in vivo study using menopausal hyperlipidemia rats, Sagunja-tang reduced retroperitoneal and perirenal fat, serum lipids, atherogenic index, cardiac risk factor, media thickness, and nonalcoholic steatohepatitis score, when compared to menopausal hyperlipidemia control rats. In HepG2 cells, Sagunja-tang significantly decreased the lipid accumulation, total cholesterol levels, and low-density/very-low-density lipoprotein levels. Moreover, Sagunja-tang reversed the methyl-β-cyclodextrin-induced decrease in the protein levels of critical molecule involved in cholesterol synthesis, sterol regulatory element binding protein-2, and low-density lipoprotein receptor and inhibited protein levels of 3-hydroxy-3-methylglutaryl coenzyme A reductase as well as activity. Phosphorylation level of AMP-activated protein kinase was stimulated by Sagunja-tang. These results suggest that Sagunja-tang has effect on inhibiting hepatic lipid accumulation through regulation of cholesterol synthesis and AMPK activity in vitro. These observations support the idea that Sagunja-tang is bioavailable both in vivo and in vitro and could be developed as a preventive and therapeutic agent of hyperlipidemia in postmenopausal females.
authors.affiliation: Korean Medicine Convergence Research Division, Korea Institute of Oriental Medicine, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 305-811, Republic of Korea.
authors.forename: Hiroe
authors.initials: H
authors.lastname: Go
authors.affiliation: Korean Medicine Convergence Research Division, Korea Institute of Oriental Medicine, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 305-811, Republic of Korea.
authors.forename: Jin Ah
authors.initials: JA
authors.lastname: Ryuk
authors.affiliation: Korean Medicine Convergence Research Division, Korea Institute of Oriental Medicine, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 305-811, Republic of Korea.
authors.forename: Hye Won
authors.initials: HW
authors.lastname: Lee
authors.affiliation: Korean Medicine Convergence Research Division, Korea Institute of Oriental Medicine, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 305-811, Republic of Korea.
authors.forename: In Sil
authors.initials: IS
authors.lastname: Park
authors.affiliation: College of Oriental Medicine, Joongbu University, 201 Daehak-Ro, Chubu-Myeon, Geumsan-Gun, ChungNam 312-702, Republic of Korea.
authors.forename: Ki-Jung
authors.initials: KJ
authors.lastname: Kil
authors.affiliation: Food and Nutrition, Obesity/Diabetes Center, Hoseo University, 165 Sechul-Ri, Bae Bang-Yup, Asan-Si, Chungcheongnam-do 336-795, Republic of Korea.
authors.forename: Sunmin
authors.initials: S
authors.lastname: Park
authors.affiliation: Dongguk University Ilsan Oriental Hospital, 27 Dongguk-Ro, Ilsandong-Gu, Goyang-Si, Gyeonggi-Do 410-773, Republic of Korea.
authors.forename: Dong Il
authors.initials: DI
authors.lastname: Kim
authors.affiliation: Korean Medicine Convergence Research Division, Korea Institute of Oriental Medicine, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 305-811, Republic of Korea.
authors.forename: Byoung Seob
authors.initials: BS
authors.lastname: Ko