title: Inhibition of leukocyte-type 12-lipoxygenase by guava tea leaves prevents development of atherosclerosis.
abstract: Oxidation of low-density lipoprotein (LDL) is one of the crucial steps for atherosclerosis development, and an essential role of leukocyte-type 12-lipoxygenase expressed in macrophages in this process has been demonstrated. The biochemical mechanism of the oxidation of circulating LDL by leukocyte-type 12-lipoxygenase in macrophages has been proposed. The major ingredients in guava tea leaves which inhibited the catalytic activity of leukocyte-type 12-lipoxygenase were quercetin and ethyl gallate. Administration of extracts from guava tea leaves to apoE-deficient mice significantly attenuated atherogenic lesions in the aorta and aortic sinus. We recently showed that Qing Shan Lu Shui inhibited the catalytic activity of leukocyte-type 12-lipoxygenase. The major components inhibiting the enzyme contained in Qing Shan Lu Shui were identified to be novel monoterpene glycosides. The anti-atherogenic effect of the tea leaves might be attributed to the inhibition of leukocyte-type 12-lipoxygenase by these components.
authors.affiliation: Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, 111 Kuboki, Soja, Okayama 719-1197, Japan. Electronic address: ytaka@fhw.oka-pu.ac.jp.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Takahashi
authors.affiliation: Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, 111 Kuboki, Soja, Okayama 719-1197, Japan.
authors.forename: Akemi
authors.initials: A
authors.lastname: Otsuki
authors.affiliation: Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, 111 Kuboki, Soja, Okayama 719-1197, Japan.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Mori
authors.affiliation: Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, 111 Kuboki, Soja, Okayama 719-1197, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Kawakami
authors.affiliation: Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, 111 Kuboki, Soja, Okayama 719-1197, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Ito
mesh_terms: D000818:Animals; D001011:Aorta / Q000378:metabolism; D001057:Apolipoproteins E / Q000235:genetics; D001092:Arachidonate 12-Lipoxygenase / Q000378:metabolism; D050197:Atherosclerosis / Q000517:prevention & control*; D004305:Dose-Response Relationship, Drug; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000737:chemistry; D006027:Glycosides / Q000737:chemistry; D020128:Inhibitory Concentration 50; D007962:Leukocytes / Q000201:enzymology*; D008077:Lipoproteins, LDL / Q000378:metabolism; D016859:Lipoxygenase Inhibitors / Q000737:chemistry*; D008264:Macrophages / Q000201:enzymology; D051379:Mice; D018345:Mice, Knockout; D039821:Monoterpenes / Q000737:chemistry; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D031647:Psidium / Q000737:chemistry*; D011794:Quercetin / Q000737:chemistry
chemical_list: D001057:Apolipoproteins E; D006027:Glycosides; D008077:Lipoproteins, LDL; D016859:Lipoxygenase Inhibitors; D039821:Monoterpenes; D010936:Plant Extracts; C048734:ethyl gallate; D005707:Gallic Acid; D011794:Quercetin; D001092:Arachidonate 12-Lipoxygenase
keywords: Arachidonic acid; Lipoxygenase; Oxidized LDL