title: Calponin-h2 is upregulated in the tissues and plasma of patients with breast cancer.
abstract: Increasing evidence has demonstrated that changes in plasma nuclear matrix proteins are specific markers of cancer. Furthermore, proteomic analysis has revealed that calponin-h2 is upregulated in human breast cancer tissue, but is absent in healthy and benign controls. However, the roles of levels of plasma calponin-h2 in the diagnosis of breast cancer and its association with clinicopathological parameters remain to be elucidated. In the present study, the plasma levels of calponin-h2 in patients with breast cancer, benign breast disease and in healthy controls were examined using an enzyme-linked immunosorbent assay. The expression levels of calponin-h2 in invasive breast cancer and normal breast tissues were measured using immunohistochemistry. Statistical analyses examined the association between the levels of plasma calponin-h2 and clinicopathological parameters. The results demonstrated that the plasma level of calponin-h2 in breast cancer was significantly higher than those in the healthy control and benign breast disease groups (P<0.05). The combination of calponin-h2, carcinoembryonic antigen, carbohydrate antigen 15-3 improved the diagnosis of breast cancer. The plasma levels of calponin-h2 PR-breast cancers was significantly higher, compared with PR+ breast cancers (P=0.033), and the plasma levels of calponin-h2 in patients with breast cancer aged >50 years was significantly higher than in patients ≤ 50 years of age (P=0.001). No association was found between the level of plasma calponin-h2 and other clinicopathological parameters of breast cancer. Taken together, these results indicated that calponin-h2 may be a useful marker of breast cancer.
authors.affiliation: Department of Clinical Laboratory, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510260, P.R. China.
authors.forename: Tianxing
authors.initials: T
authors.lastname: Ji
authors.affiliation: Department of VIP Obstetrics, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510260, P.R. China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Ma
authors.affiliation: Department of Pathology, Guangdong Women and Children Hospital, Guangzhou, Guangdong 511442, P.R. China.
authors.forename: Leijun
authors.initials: L
authors.lastname: Huo
authors.affiliation: Department of Clinical Laboratory, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510140, P.R. China.
authors.forename: Xuguang
authors.initials: X
authors.lastname: Guo
authors.affiliation: Department of Clinical Laboratory, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510260, P.R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Chen
authors.affiliation: Department of Clinical Laboratory, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510260, P.R. China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhou
mesh_terms: D000328:Adult; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000032:analysis / Q000097:blood; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000503:physiopathology*; D002135:Calcium-Binding Proteins / Q000032:analysis / Q000097:blood / Q000235:genetics*; D002272:Carcinoembryonic Antigen / Q000032:analysis / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008840:Microfilament Proteins / Q000032:analysis / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D018396:Mucin-1 / Q000032:analysis / Q000097:blood; D012372:ROC Curve; D015854:Up-Regulation; D000096985:Calponins
chemical_list: D014408:Biomarkers, Tumor; D002135:Calcium-Binding Proteins; D002272:Carcinoembryonic Antigen; D008840:Microfilament Proteins; D018396:Mucin-1; D000096985:Calponins