title: Altered long noncoding RNA expression profiles in the myocardium of rats with ischemic heart failure.
abstract: OBJECTIVE
  Despite significant advances in the treatment of coronary artery disease, the prevalence of ischemic heart failure is still increasing rapidly. Long noncoding RNAs are a novel class of gene regulators and may contribute to disease cause. The aim of the present study was to investigate the expression profiles of long noncoding RNAs and their potential functional roles in ischemic heart failure.
  
  
  METHODS
  We applied a well-established ischemic heart failure rat model and performed long noncoding RNA microarray experiments on the left ventricular tissue of rats with ischemic heart failure and under sham control. Differentially expressed long noncoding RNAs and mRNAs were identified through fold-change filtering. Bioinformatic analyses were performed to predict the potential biological roles of key long noncoding RNAs.
  
  
  RESULTS
  We found that 1197 long noncoding RNAs and 2066 mRNAs were upregulated, whereas 1403 long noncoding RNAs and 2871 mRNAs were downregulated in failing hearts (fold-change > 2.0). We also identified 331 pairs of differentially expressed long noncoding RNAs and nearby coding genes, which contained 291 long noncoding RNAs and 296 mRNAs. Expression levels of four long noncoding RNA-mRNA pairs, which might be involved in the pathogenesis of ischemic heart failure were confirmed by quantitative real-time PCR.
  
  
  CONCLUSIONS
  Our study identified a set of long noncoding RNAs that were aberrantly expressed in rats with ischemic heart failure and might be involved in the pathogenesis of ischemic heart failure. The results of our study may provide a novel perspective for better understanding the molecular basis of ischemic heart failure.
authors.affiliation: aDepartment of Cardiology, the First Affiliated Hospital of Nanjing Medical University bDepartment of Geriatrics, the Second Affiliated Hospital of Nanjing Medical University, Nanjing, China *Wei Gao and Ze-Mu Wang contributed equally to this work.
authors.forename: Wei
authors.initials: W
authors.lastname: Gao
authors.forename: Ze-Mu
authors.initials: ZM
authors.lastname: Wang
authors.forename: Meng
authors.initials: M
authors.lastname: Zhu
authors.forename: Xiao-Qing
authors.initials: XQ
authors.lastname: Lian
authors.forename: Huan
authors.initials: H
authors.lastname: Zhao
authors.forename: Di
authors.initials: D
authors.lastname: Zhao
authors.forename: Zhi-Jian
authors.initials: ZJ
authors.lastname: Yang
authors.forename: Xiang
authors.initials: X
authors.lastname: Lu
authors.forename: Lian-Sheng
authors.initials: LS
authors.lastname: Wang
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation; D063990:Gene Ontology; D020022:Genetic Predisposition to Disease; D006333:Heart Failure / Q000235:genetics*; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D062085:RNA, Long Noncoding / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D017207:Rats, Sprague-Dawley
chemical_list: D062085:RNA, Long Noncoding; D012333:RNA, Messenger