title: Biogenesis of protein bodies during legumin accumulation in developing olive (Olea europaea L.) seed.
abstract: Much of our current knowledge about seed development and differentiation regarding reserves synthesis and accumulation come from monocot (cereals) plants. Studies in dicotyledonous seeds differentiation are limited to a few species and in oleaginous species are even scarcer despite their agronomic and economic importance. We examined the changes accompanying the differentiation of olive endosperm and cotyledon with a focus on protein bodies (PBs) biogenesis during legumin protein synthesis and accumulation, with the aim of getting insights and a better understanding of the PBs' formation process. Cotyledon and endosperm undergo differentiation during seed development, where an asynchronous time-course of protein synthesis, accumulation, and differential PB formation patterns was found in both tissues. At the end of seed maturation, a broad population of PBs, particularly in cotyledon cells, was distinguishable in terms of number per cell and morphometric and cytochemical features. Olive seed development is a tissue-dependent process characterized by differential rates of legumin accumulation and PB formation in the main tissues integrating seed. One of the main features of the impressive differentiation process is the specific formation of a broad group of PBs, particularly in cotyledon cells, which might depend on selective accumulation and packaging of proteins and specific polypeptides into PBs. The nature and availability of the major components detected in the PBs of olive seed are key parameters in order to consider the potential use of this material as a suitable source of carbon and nitrogen for animal or even human use.
authors.affiliation: The UWA Institute of Agriculture, The University of Western Australia, 35 Stirling Highway, Crawley, Perth, WA, 6009, Australia. jcjimenezl75@gmail.com.|Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, National Council for Scientific Research (CSIC), Profesor Albareda 1, Granada, 18008, Spain. jcjimenezl75@gmail.com.
authors.forename: Jose C
authors.initials: JC
authors.lastname: Jimenez-Lopez
authors.affiliation: Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, National Council for Scientific Research (CSIC), Profesor Albareda 1, Granada, 18008, Spain.|Department of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Toruń, 87-100, Poland.|Centre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University, Toruń, 87-100, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Zienkiewicz
authors.affiliation: Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, National Council for Scientific Research (CSIC), Profesor Albareda 1, Granada, 18008, Spain.|Department of Cell Biology, Nicolaus Copernicus University, Toruń, 87-100, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Zienkiewicz
authors.affiliation: Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, National Council for Scientific Research (CSIC), Profesor Albareda 1, Granada, 18008, Spain.
authors.forename: Juan D
authors.initials: JD
authors.lastname: Alché
authors.affiliation: Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, National Council for Scientific Research (CSIC), Profesor Albareda 1, Granada, 18008, Spain. mariaisabel.rodriguez@eez.csic.es.
authors.forename: Maria I
authors.initials: MI
authors.lastname: Rodríguez-García
mesh_terms: D018548:Cotyledon / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D056625:Endosperm / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D031658:Olea / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D010940:Plant Proteins / Q000096:biosynthesis*; D000097793:Legumins
chemical_list: D010940:Plant Proteins; D000097793:Legumins
keywords: Cotyledon; Development; Endosperm; Legumin; Protein body; Seed