title: Biothiol-triggered, self-disassembled silica nanobeads for intracellular drug delivery.
abstract: Silica-based nanomaterials have demonstrated great potential in biomedical applications due to their chemical inertness. However, the degradability and endosomal trapping issues remain as rate-limiting barriers during their innovation. In this study, we provide a simple yet novel sol-gel approach to construct the redox-responsive silica nanobeads (ReSiNs), which could be rapidly disassembled upon redox gradient for intracellular drug delivery. The disulfide-linked scaffold of the nanobead was synthesized by employing the dithiobis-(succinimidyl propionate) to bridge (3-aminopropyl)-trimethoxysilane. Such silica matrix could be efficiently disrupted in response to intracellular glutathione, resulting in drug release and collapse of entire nanocarrier. Moreover, the ReSiNs exhibited insignificant cytotoxicity before and after the degradation. These results indicated the potential of using ReSiNs as a novel silica-based, biothiol-degradable nanoplatform for future drug delivery.
authors.affiliation: Department of Applied Chemistry, National Chiao-Tung University, No. 1001 Ta-Hsueh Road, Hsinchu 30010, Taiwan.
authors.forename: Xin-Chun
authors.initials: XC
authors.lastname: Huang
authors.affiliation: Department of Applied Chemistry, National Chiao-Tung University, No. 1001 Ta-Hsueh Road, Hsinchu 30010, Taiwan.
authors.forename: Li-Bang
authors.initials: LB
authors.lastname: Wu
authors.affiliation: Institute of Molecular Science, National Chiao-Tung University, No. 1001 Ta-Hsueh Road, Hsinchu 30010, Taiwan.
authors.forename: Jen-Fang
authors.initials: JF
authors.lastname: Hsu
authors.affiliation: Department of Applied Chemistry, National Chiao-Tung University, No. 1001 Ta-Hsueh Road, Hsinchu 30010, Taiwan; Institute of Molecular Science, National Chiao-Tung University, No. 1001 Ta-Hsueh Road, Hsinchu 30010, Taiwan.
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Shigeto
authors.affiliation: Department of Applied Chemistry, National Chiao-Tung University, No. 1001 Ta-Hsueh Road, Hsinchu 30010, Taiwan; Institute of Molecular Science, National Chiao-Tung University, No. 1001 Ta-Hsueh Road, Hsinchu 30010, Taiwan. Electronic address: hyhsu99@nctu.edu.tw.
authors.forename: Hsin-Yun
authors.initials: HY
authors.lastname: Hsu
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D003460:Crystallization / Q000379:methods; D003692:Delayed-Action Preparations / Q000138:chemical synthesis* / Q000633:toxicity; D056945:Hep G2 Cells; D006801:Humans; D053769:Nanocapsules / Q000737:chemistry* / Q000627:therapeutic use / Q000648:ultrastructure; D054118:Nanospheres / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D010316:Particle Size; D012822:Silicon Dioxide / Q000737:chemistry* / Q000633:toxicity; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D003692:Delayed-Action Preparations; D053769:Nanocapsules; D013438:Sulfhydryl Compounds; D012822:Silicon Dioxide
keywords: Biothiols; Drug delivery; Self-disassembly; Silica nanoparticles; Sol–gel processes