title: In vitro antiparasitic activity of microbial pigments and their combination with phytosynthesized metal nanoparticles.
abstract: Bioactive pigments were extracted and purified from cultures of Serratia marcescens and Chromobacterium violaceum. Spectroscopic, FTIR, and HPLC analyses showed prodigiosin and violacein as the principle molecules in the extract. Bioactive microbial pigments prodigiosin, violacein and their combinations with phytosynthesized silver and gold nanoparticles were studied for in vitro growth inhibition of Plasmodium falciparum and Trypanosoma brucei gambiense. Prodigiosin was found to be more effective than violacein for inhibition of both parasites in vitro. Specifically, combinations of the microbial pigment prodigiosin with metal nanoparticles showed a significant decrease in the IC50 values on both parasites (2.7 to 3.6 fold) without increase of cytotoxicity upon mammalian cells. The data may be useful for the microbial pigment based drug designing.
authors.affiliation: School of Life Sciences, North Maharashtra University, Jalgaon, 425001 Maharashtra, India.
authors.forename: Suryawanshi
authors.initials: S
authors.lastname: Rahul
authors.affiliation: School of Life Sciences, North Maharashtra University, Jalgaon, 425001 Maharashtra, India.
authors.forename: Patil
authors.initials: P
authors.lastname: Chandrashekhar
authors.affiliation: School of Life Sciences, North Maharashtra University, Jalgaon, 425001 Maharashtra, India.
authors.forename: Borase
authors.initials: B
authors.lastname: Hemant
authors.affiliation: School of Life Sciences, North Maharashtra University, Jalgaon, 425001 Maharashtra, India.
authors.forename: Salunke
authors.initials: S
authors.lastname: Bipinchandra
authors.affiliation: Muséum National d'Histoire Naturelle, UMR 7245 CNRS Molécules de Communication et Adaptation des Microorganismes, Paris, France.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Mouray
authors.affiliation: Muséum National d'Histoire Naturelle, UMR 7245 CNRS Molécules de Communication et Adaptation des Microorganismes, Paris, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Grellier
authors.affiliation: School of Life Sciences, North Maharashtra University, Jalgaon, 425001 Maharashtra, India. Electronic address: satish.patil7@gmail.com.
authors.forename: Patil
authors.initials: P
authors.lastname: Satish
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D000962:Antimalarials / Q000494:pharmacology; D002861:Chromobacterium / Q000737:chemistry; D004337:Drug Carriers; D006046:Gold; D006801:Humans; D007211:Indoles / Q000737:chemistry / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D053768:Metal Nanoparticles; D010860:Pigments, Biological / Q000008:administration & dosage / Q000494:pharmacology*; D010944:Plants / Q000378:metabolism; D010963:Plasmodium falciparum / Q000187:drug effects*; D011353:Prodigiosin / Q000737:chemistry / Q000494:pharmacology*; D012705:Serratia / Q000737:chemistry; D012834:Silver; D014344:Trypanocidal Agents / Q000494:pharmacology; D014347:Trypanosoma brucei gambiense / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D000962:Antimalarials; D004337:Drug Carriers; D007211:Indoles; D010860:Pigments, Biological; D014344:Trypanocidal Agents; D012834:Silver; D006046:Gold; D011353:Prodigiosin; C063155:violacein
keywords: Antimalarial; Plasmodium falciparum; Prodigiosin; Trypanosoma brucei gambiense; Violacein