title: [Simultaneous determination of flavones and saponins of Rhizoma Anemarrhenae by HPLC-DAD-ELSD].
abstract: This study is to establish an HPLC-DAD-ELSD method for simultaneous determination of 5 flavones and saponins in Rhizoma Anemarrhenae including neo-mangiferin, mangiferin, timosaponin B II, timosaponin B III and timosaponin A III. Samples were analyzed on a Merck Purospher STAR column(4.6 mm x 250 mm, 5 μm). The mobile phase consisted of acetonitrile( A) and 0. 1% formic acid (B) with gradient elution at a flow rate of 1.0 mL · min(-1). The column temperature was set at 40 °C. The DAD detector wavelength was set at 254 nm. The ELSD conditions were as follows: the nebulizing gas flow rate was 2.0 L · min(-1) and temperature of drift tube was 105 °C. The volume was 10 μL. The five compounds were well separated with good linear correlations. The mean recoveries were between 102.0%-104.0%. This method was quick and reliable which provides a foundation for quality control of R. Anemarrhenae.
authors.forename: Xiao-Nan
authors.initials: XN
authors.lastname: Su
authors.forename: De
authors.initials: D
authors.lastname: Ji
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Zhou
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Wang
authors.forename: Wen-Yi
authors.initials: WY
authors.lastname: Zang
authors.forename: Chun-Qin
authors.initials: CQ
authors.lastname: Mao
authors.forename: Tu-Lin
authors.initials: TL
authors.lastname: Lu
mesh_terms: D031403:Anemarrhena / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D047309:Flavones / Q000032:analysis*; D027343:Rhizome / Q000737:chemistry; D012503:Saponins / Q000032:analysis*
chemical_list: D004365:Drugs, Chinese Herbal; D047309:Flavones; D012503:Saponins