title: Hypolipidemic effects and safety of Lactobacillus reuteri 263 in a hamster model of hyperlipidemia.
abstract: We aimed to verify the beneficial effects of probiotic strain Lactobacillus reuteri 263 (Lr263) on hypolipidemic action in hamsters with hyperlipidemia induced by a 0.2% cholesterol and 10% lard diet (i.e., high-cholesterol diet (HCD)). Male Golden Syrian hamsters were randomly divided into two groups: normal (n = 8), standard diet (control), and experimental (n = 32), a HCD. After a two-week induction followed by a six-week supplementation with Lr263, the 32 hyperlipidemic hamsters were divided into four groups (n = 8 per group) to receive vehicle or Lr263 by oral gavage at 2.1, 4.2, or 10.5 × 10(9) cells/kg/day for 6 weeks, designated the HCD, 1X, 2X and 5X groups, respectively. The efficacy and safety of Lr263 supplementation were evaluated by lipid profiles of serum, liver and feces and by clinical biochemistry and histopathology. HCD significantly increased serum levels of total cholesterol (TC), triacylglycerol (TG) cholesterol, high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C), LDL-C/HDL-C ratio, hepatic and fetal TC and TG levels, and degree of fatty liver as compared with controls. Lr263 supplementation dose dependently increased serum HDL-C level and decreased serum TC, TG, LDL-C levels, LDL-C/HDL-C ratio, hepatic TC and TG levels, and fecal TG level. In addition, Lr263 supplementation had few subchronic toxic effects. Lr263 could be a potential agent with a hypolipidemic pharmacological effect.
authors.affiliation: Graduate Institute of Athletics and Coaching Science, National Taiwan Sport University, Taoyuan 33301, Taiwan. 1010503@ntsu.edu.tw.
authors.forename: Wen-Ching
authors.initials: WC
authors.lastname: Huang
authors.affiliation: Graduate Institute of Sports Science, National Taiwan Sport University, Taoyuan 33301, Taiwan. 1021302@ntsu.edu.tw.
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Chen
authors.affiliation: Graduate Institute of Athletics and Coaching Science, National Taiwan Sport University, Taoyuan 33301, Taiwan. kevinkan@tmu.edu.tw.|Center for Liberal Arts, Taipei Medical University, Taipei 11031, Taiwan. kevinkan@tmu.edu.tw.
authors.forename: Nai-Wen
authors.initials: NW
authors.lastname: Kan
authors.affiliation: College of Exercise and Health Sciences, National Taiwan Sport University, Taoyuan 33301, Taiwan. 1031213@ntsu.edu.tw.|Division of Physical Medicine and Rehabilitation, Lo-Hsu foundation, Inc., Lotung Poh-Ai Hospital, Yilan 26546, Taiwan. 1031213@ntsu.edu.tw.
authors.forename: Chun-Sheng
authors.initials: CS
authors.lastname: Ho
authors.affiliation: Department of Neurosurgery, Taipei Medical University-WanFang Hospital, Taipei 11696, Taiwan. nsweili@gmail.com.
authors.forename: Li
authors.initials: L
authors.lastname: Wei
authors.affiliation: Department of Food Science, Nutrition, and Nutraceutical Biotechnology, Shih Chien University, Taipei 10462, Taiwan. llfonly_520@hotmail.com.
authors.forename: Ching-Hung
authors.initials: CH
authors.lastname: Chan
authors.affiliation: Department of Food Science, Nutrition, and Nutraceutical Biotechnology, Shih Chien University, Taipei 10462, Taiwan. maggieh@g2.usc.edu.tw.
authors.forename: Hui-Yu
authors.initials: HY
authors.lastname: Huang
authors.affiliation: Graduate Institute of Sports Science, National Taiwan Sport University, Taoyuan 33301, Taiwan. john5523@ntsu.edu.tw.|College of Exercise and Health Sciences, National Taiwan Sport University, Taoyuan 33301, Taiwan. john5523@ntsu.edu.tw.
authors.forename: Chi-Chang
authors.initials: CC
authors.lastname: Huang
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000097:blood*; D006224:Cricetinae; D059305:Diet, High-Fat; D004195:Disease Models, Animal; D005234:Fatty Liver / Q000517:prevention & control; D005243:Feces / Q000737:chemistry; D006949:Hyperlipidemias / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism; D000960:Hypolipidemic Agents / Q000627:therapeutic use*; D052200:Limosilactobacillus reuteri; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008647:Mesocricetus; D019936:Probiotics / Q000627:therapeutic use*; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D000960:Hypolipidemic Agents; D014280:Triglycerides; D002784:Cholesterol
keywords: cholesterol; high-cholesterol diet; hypolipidemic; lipid-lowering; triglyceride