title: Therapeutic effects of lipo-prostaglandin E1 on angiogenesis and neurogenesis after ischemic stroke in rats.
abstract: Previous studies have demonstrated that prostaglandin E1 (PGE1) has a neuroprotective effect on cerebral ischemia. However, it remains unknown whether PGE1 promotes angiogenesis and neurogenesis after ischemic stroke. In this study, adult male Sprague-Dawley rats were subjected to permanently distal middle cerebral artery occlusion (MCAO). Rats were treated with lipo-prostaglandin E1(lipo-PGE1, 10 μg/kg/d) or the same volume of 0.9% saline starting 24 hours after MCAO daily for 6 consecutive days. All rats were injected 5'-bromo-2'-deoxyuridine (BrdU, 50 mg/kg) intraperitoneally every 12 hours for 3 consecutive days before being sacrificed. At 7 and 14 days after MCAO or sham-operation, rats were sacrificed. Post-stroke neurological outcome, infarction volume, angiogenesis and neurogenesis were evaluated. Treatment with lipo-PGE1 significantly increased the vascular density in the peri-infarct areas at 7 and 14 days after MCAO. The lipo-PGE1 treatment significantly enhanced the proliferation and migration of endogenous neural stem cells in the ipsilateral subventricular zone. The neural stem cells associated with blood vessels closely within a neurovascular niche in lipo-PGE1-treated rats after stroke. The lipo-PGE1 treatment also significantly improved the neurological recovery after MCAO. These results indicate that treatment with lipo-PGE1 promotes post-stroke angiogenesis, neurogenesis and their interaction, which would contribute to neurological recovery after cerebral infarction. Our study provides novel experimental evidences for the neuroprotective roles of PGE1 in ischemic stroke.
authors.affiliation: a 1 Department of Neurology, Guangzhou Red Cross Hospital, Medical College , Jinan University , Guangzhou , P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Ling
authors.affiliation: a 1 Department of Neurology, Guangzhou Red Cross Hospital, Medical College , Jinan University , Guangzhou , P.R. China.
authors.forename: Suping
authors.initials: S
authors.lastname: Zhang
authors.affiliation: a 1 Department of Neurology, Guangzhou Red Cross Hospital, Medical College , Jinan University , Guangzhou , P.R. China.
authors.forename: Zhangge
authors.initials: Z
authors.lastname: Ji
authors.affiliation: a 1 Department of Neurology, Guangzhou Red Cross Hospital, Medical College , Jinan University , Guangzhou , P.R. China.
authors.forename: Huihong
authors.initials: H
authors.lastname: Huang
authors.affiliation: b 2 Department of Ophthalmology , The People's Hospital of Guangxi Zhuang Autonomous Region , Nanning , P.R. China.
authors.forename: Gang
authors.initials: G
authors.lastname: Yao
authors.affiliation: a 1 Department of Neurology, Guangzhou Red Cross Hospital, Medical College , Jinan University , Guangzhou , P.R. China.
authors.forename: Muzhen
authors.initials: M
authors.lastname: Wang
authors.affiliation: a 1 Department of Neurology, Guangzhou Red Cross Hospital, Medical College , Jinan University , Guangzhou , P.R. China.
authors.forename: Rui
authors.initials: R
authors.lastname: He
authors.affiliation: a 1 Department of Neurology, Guangzhou Red Cross Hospital, Medical College , Jinan University , Guangzhou , P.R. China.
authors.forename: Wanqing
authors.initials: W
authors.lastname: Deng
authors.affiliation: c 3 Department of Pathology, Guangzhou Red Cross Hospital, Medical College , Jinan University , Guangzhou , P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Fang
mesh_terms: D000527:Alprostadil / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D002545:Brain Ischemia / Q000188:drug therapy* / Q000503:physiopathology; D004195:Disease Models, Animal; D008297:Male; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D058953:Neural Stem Cells / Q000187:drug effects / Q000502:physiology; D055495:Neurogenesis / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000187:drug effects / Q000502:physiology; D020521:Stroke / Q000188:drug therapy* / Q000503:physiopathology
chemical_list: D018696:Neuroprotective Agents; D000527:Alprostadil
keywords: angiogenesis; ischemic stroke; neurogenesis