title: Paeoniflorin regulates the function of human peripheral blood mononuclear cells stimulated by rhIL-1β by up-regulating Treg expression.
abstract: The aim of the present study was to investigate the effect of paeoniflorin (Pae) on recombinant human interleukin-1β (rhIL-1β)-stimulated human peripheral blood mononuclear cells (PBMCs) in vitro. PBMCs were collected by Ficoll density gradient centrifugation and were co-cultured with rhIL-1β for different time periods. The proliferation response was determined by a cell counting kit-8 (CCK-8) assay. The production of IL-17 and IL-10 was measured by enzyme-linked immunosorbent assay (ELISA). The percentage of CD4(+)CD25(+)Foxp3(+) regulatory T cells (Treg) was detected by flow cytometry analysis. These results indicated that rhIL-1β stimulation induced the proliferation of PBMCs in a concentration- and time-dependent manner; it also increased the level of IL-17 and decreased the level of IL-10 in a concentration-dependent manner. The flow cytometry analysis demonstrated that the stimulation of rhIL-1β significantly downregulated the percentage of CD4(+)CD25(+)Foxp3(+) Treg in CD4(+) T cells. However, administration of Pae significantly suppressed the proliferation response of rhIL-1β-induced PBMCs and regulated the secretion function of IL-17 and IL-10. Additional experiments demonstrated that Pae treatment significantly reduced rhIL-1β-induced decreases in PBMCs CD4(+)CD25(+)Foxp3(+) subpopulation numbers. These results suggest that the anti-inflammatory action of Pae is attributable to its regulation of IL-17/IL-10 secretion and Treg expression.
authors.affiliation: Key Laboratory of Anti-inflammatory and Immune Medicine of the Education Ministry of China, Institute of Clinical Pharmacology, Anhui Medical University , Hefei , China.
authors.forename: Xing
authors.initials: X
authors.lastname: Dai
authors.forename: Li-Wen
authors.initials: LW
authors.lastname: Wang
authors.forename: Xiao-Yi
authors.initials: XY
authors.lastname: Jia
authors.forename: Yan
authors.initials: Y
authors.lastname: Chang
authors.forename: Hua-Xun
authors.initials: HX
authors.lastname: Wu
authors.forename: Chun
authors.initials: C
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
mesh_terms: D000293:Adolescent; D000328:Adult; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000276:immunology; D005960:Glucosides / Q000494:pharmacology*; D006801:Humans; D016753:Interleukin-10 / Q000276:immunology; D020381:Interleukin-17 / Q000276:immunology; D053583:Interleukin-1beta / Q000494:pharmacology*; D008297:Male; D039821:Monoterpenes / Q000494:pharmacology*; D050378:T-Lymphocytes, Regulatory / Q000166:cytology / Q000276:immunology*; D006027:Glycosides
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D005960:Glucosides; D016753:Interleukin-10; D020381:Interleukin-17; D053583:Interleukin-1beta; D039821:Monoterpenes; C508585:IL1B protein, human; C015423:peoniflorin; D006027:Glycosides
keywords: Human peripheral blood mononuclear cells; RhIL-1β; inflammation; paeoniflorin; treg