title: Improved surveillance for carbapenem-resistant Enterobacteriaceae using chromogenic media with a broth enrichment.
abstract: In this study, 238 rectal screening swabs for carbapenem-resistant Enterobacteriaceae (CRE) were compared using chromID CARBA plates and the CDC reference method. Direct inoculation of chromID CARBA plates only identified 53% of CRE, whereas meropenem broth enrichment increased the positive agreement to 88% and identified additional cases of CRE. Additionally, the chromID CARBA method allowed for faster results and decreased the number of colonies further tested.
authors.affiliation: Clinical Microbiology Laboratory, Hospital of the University of Pennsylvania, Philadelphia, PA, 19104; Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104.
authors.forename: Laurel
authors.initials: L
authors.lastname: Glaser
authors.affiliation: Clinical Microbiology Laboratory, Hospital of the University of Pennsylvania, Philadelphia, PA, 19104.
authors.forename: Kathleen
authors.initials: K
authors.lastname: Andreacchio
authors.affiliation: Clinical Microbiology Laboratory, Hospital of the University of Pennsylvania, Philadelphia, PA, 19104.
authors.forename: Mathilda
authors.initials: M
authors.lastname: Lyons
authors.affiliation: Clinical Microbiology Laboratory, Hospital of the University of Pennsylvania, Philadelphia, PA, 19104; Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104. Electronic address: kevin.alby@uphs.upenn.edu.
authors.forename: Kevin
authors.initials: K
authors.lastname: Alby
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000032:analysis*; D001431:Bacteriological Techniques / Q000379:methods*; D015780:Carbapenems / Q000494:pharmacology; D002863:Chromogenic Compounds / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry*; D004755:Enterobacteriaceae / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification*; D062665:Epidemiological Monitoring; D006801:Humans; D012007:Rectum / Q000382:microbiology; D012680:Sensitivity and Specificity; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000032:analysis*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D002863:Chromogenic Compounds; D003470:Culture Media; D001618:beta-Lactamases; C063912:carbapenemase
keywords: Antibiotic resistance; CRE; Surveillance; chromID