title: Metaproteomics reveals major microbial players and their biodegradation functions in a large-scale aerobic composting plant.
abstract: Composting is an appropriate management alternative for municipal solid waste; however, our knowledge about the microbial regulation of this process is still scare. We employed metaproteomics to elucidate the main biodegradation pathways in municipal solid waste composting system across the main phases in a large-scale composting plant. The investigation of microbial succession revealed that Bacillales, Actinobacteria and Saccharomyces increased significantly with respect to abundance in composting process. The key microbiologic population for cellulose degradation in different composting stages was different. Fungi were found to be the main producers of cellulase in earlier phase. However, the cellulolytic fungal communities were gradually replaced by a purely bacterial one in active phase, which did not support the concept that the thermophilic fungi are active through the thermophilic phase. The effective decomposition of cellulose required the synergy between bacteria and fungi in the curing phase.
authors.affiliation: College of Life Sciences, Northeast Agricultural University, Harbin, 150030, China.|Innovation Base of Groundwater and Environmental System Engineering, Chinese Research Academy of Environmental Science, Beijing, 100012, China.
authors.forename: Dongming
authors.initials: D
authors.lastname: Liu
authors.affiliation: Innovation Base of Groundwater and Environmental System Engineering, Chinese Research Academy of Environmental Science, Beijing, 100012, China.
authors.forename: Mingxiao
authors.initials: M
authors.lastname: Li
authors.affiliation: Innovation Base of Groundwater and Environmental System Engineering, Chinese Research Academy of Environmental Science, Beijing, 100012, China.
authors.forename: Beidou
authors.initials: B
authors.lastname: Xi
authors.affiliation: College of Life Sciences, Northeast Agricultural University, Harbin, 150030, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: College of Life Sciences, Northeast Agricultural University, Harbin, 150030, China.
authors.forename: Zimin
authors.initials: Z
authors.lastname: Wei
authors.affiliation: College of Life Sciences, Northeast Agricultural University, Harbin, 150030, China.|Innovation Base of Groundwater and Environmental System Engineering, Chinese Research Academy of Environmental Science, Beijing, 100012, China.
authors.forename: Caihong
authors.initials: C
authors.lastname: Song
authors.affiliation: Innovation Base of Groundwater and Environmental System Engineering, Chinese Research Academy of Environmental Science, Beijing, 100012, China.
authors.forename: Chaowei
authors.initials: C
authors.lastname: Zhu
mesh_terms: D000332:Aerobiosis; D001426:Bacterial Proteins / Q000032:analysis*; D058448:Biota; D001711:Biotransformation; D005656:Fungal Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis*; D012037:Refuse Disposal; D012987:Soil; D012988:Soil Microbiology; D062611:Solid Waste
chemical_list: D001426:Bacterial Proteins; D005656:Fungal Proteins; D020543:Proteome; D012987:Soil; D062611:Solid Waste