title: Plasma and mitochondrial membrane perturbation induced by aluminum in human peripheral blood lymphocytes.
abstract: Aluminum is a redox-inert element that could induce cell damage via activation of oxidative stress. In this work, the effect of aluminum on different cellular compartments of human peripheral blood lymphocytes was studied. The presence of aluminum induced a lipid peroxidation and physico-chemical modifications at the membrane level. A decrease in fluorescence anisotropy of TMA-DPH and in the polarity of the lipid bilayer with a concomitant shift toward a gel phase was observed, while the pyrene excimerization coefficient (Kex) increased. Flow cytometry measurements, using JC-1, Rhodamine 123 and H2-DCFDA as fluorescent probes, indicated that aluminum induces a slight mitochondrial membrane depolarization that was associated with a moderate increase in reactive oxygen species production. A significative influence on these parameters was measured only at high aluminum concentration.
authors.affiliation: Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus, Belarus.
authors.forename: Aliaksandra Sergeevna
authors.initials: AS
authors.lastname: Skarabahatava
authors.affiliation: Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus, Belarus.
authors.forename: Ludmila Michaylovna
authors.initials: LM
authors.lastname: Lukyanenko
authors.affiliation: Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus, Belarus.
authors.forename: Ekaterina Ivanovna
authors.initials: EI
authors.lastname: Slobozhanina
authors.affiliation: School of Advanced Studies "Ageing and Nutrition", University of Camerino, Italy.
authors.forename: Maria Letizia
authors.initials: ML
authors.lastname: Falcioni
authors.affiliation: Department of Clinical Dental Sciences, Polytechnic University of Marche, via Ranieri 60131, Ancona, Italy.
authors.forename: Patrick
authors.initials: P
authors.lastname: Orlando
authors.affiliation: Department of Clinical Dental Sciences, Polytechnic University of Marche, via Ranieri 60131, Ancona, Italy.
authors.forename: Sonia
authors.initials: S
authors.lastname: Silvestri
authors.affiliation: Department of Clinical Dental Sciences, Polytechnic University of Marche, via Ranieri 60131, Ancona, Italy. Electronic address: l.tiano@univpm.it.
authors.forename: Luca
authors.initials: L
authors.lastname: Tiano
authors.affiliation: School of Pharmacy and Health Products, University of Camerino, Italy.
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Falcioni
mesh_terms: D000328:Adult; D000535:Aluminum / Q000097:blood / Q000633:toxicity*; D001562:Benzimidazoles / Q000378:metabolism; D002232:Carbocyanines / Q000378:metabolism; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects*; D002478:Cells, Cultured; D005454:Fluorescence Polarization; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008875:Middle Aged; D051336:Mitochondrial Membranes / Q000737:chemistry / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D020112:Rhodamine 123 / Q000378:metabolism
chemical_list: D001562:Benzimidazoles; D002232:Carbocyanines; D017382:Reactive Oxygen Species; D020112:Rhodamine 123; C068624:5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine; D000535:Aluminum
keywords: Aluminum; Human lymphocytes; Lipid peroxides; Membrane fluidity; Mitochondrial membrane depolarization; Reactive oxygen species