title: miR-103 Regulates Oxidative Stress by Targeting the BCL2/Adenovirus E1B 19 kDa Interacting Protein 3 in HUVECs.
abstract: Oxidative stress plays a critical role in cardiovascular diseases. Salidroside, a glycoside from Rhodiola rosea, has been used as an antioxidative therapy for oxidative injury in cardiac diseases. However, the mechanism underlying its antioxidant effect needs to be elucidated. Treatment of HUVECs with H2O2 significantly decreased the expression of miR-103 in a dose- and time-dependent manner, whereas pretreatment with salidroside significantly inhibited this decrease. Subsequent analysis showed that overexpression of miR-103 abrogated cell activity and ROS production induced by H2O2. Bcl2/adenovirus E1B 19 kDa interacting protein 3 (BNIP3) was determined to be a novel miR-103 target in HUVECs. Interestingly, H2O2 treatment upregulated BNIP3 expression; in turn, this effect was inhibited by pretreatment with salidroside. Further studies confirmed that the knockdown of BNIP3 enhanced cell activity and suppressed the ROS production induced by H2O2. These results demonstrated for the first time that salidroside protects HUVECs in part by upregulating the expression of miR-103, which mediates BNIP3 downregulation and plays an important role in the cytoprotective actions.
authors.affiliation: Department of Cardiology, Shanghai Pudong New Area Gongli Hospital, Shanghai 200135, China.
authors.forename: Mao-Chun
authors.initials: MC
authors.lastname: Xu
authors.affiliation: Department of Cardiology, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors.forename: Xiu-Fang
authors.initials: XF
authors.lastname: Gao
authors.affiliation: Department of Cardiology, Shanghai Pudong New Area Gongli Hospital, Shanghai 200135, China.
authors.forename: Changwu
authors.initials: C
authors.lastname: Ruan
authors.affiliation: Department of Cardiology, Shanghai Pudong New Area Gongli Hospital, Shanghai 200135, China.
authors.forename: Zhi-Ru
authors.initials: ZR
authors.lastname: Ge
authors.affiliation: Department of Cardiology, Shanghai Pudong New Area Gongli Hospital, Shanghai 200135, China.
authors.forename: Ji-De
authors.initials: JD
authors.lastname: Lu
authors.affiliation: Department of Cardiology, Shanghai Pudong New Area Gongli Hospital, Shanghai 200135, China.
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, Shanghai Pudong New Area Gongli Hospital, Shanghai 200135, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, Shanghai Pudong New Area Gongli Hospital, Shanghai 200135, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors.forename: Hai-Ming
authors.initials: HM
authors.lastname: Shi
mesh_terms: D020413:3' Untranslated Regions; D017209:Apoptosis / Q000187:drug effects; D001483:Base Sequence; D015536:Down-Regulation / Q000187:drug effects; D005960:Glucosides / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008565:Membrane Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000494:pharmacology*; D011518:Proto-Oncogene Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D029771:Rhodiola / Q000378:metabolism; D016415:Sequence Alignment; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D020413:3' Untranslated Regions; C090121:BNIP3 protein, human; D005960:Glucosides; C544749:MIRN103 microRNA, human; D008565:Membrane Proteins; D035683:MicroRNAs; D010636:Phenols; D011518:Proto-Oncogene Proteins; D034741:RNA, Small Interfering; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; C009172:rhodioloside