title: A Protocol to Collect Specific Mouse Skeletal Muscles for Metabolomics Studies.
abstract: Due to the highly sensitive nature of metabolic states, the quality of metabolomics data depends on the suitability of the experimental procedure. Metabolism could be affected by factors such as the method of euthanasia of the animals and the sample collection procedures. The effects of these factors on metabolites are tissue-specific. Thus, it is important to select proper methods to sacrifice the animal and appropriate procedures for collecting samples specific to the tissue of interest. Here, we present our protocol to collect specific mouse skeletal muscles with different fiber types for metabolomics studies. We also provide a protocol to measure lactate levels in tissue samples as a way to estimate the metabolic state in collected samples.
authors.affiliation: Department of Bioengineering, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, 92093-0412, USA. zgan@eng.ucsd.edu.
authors.forename: Zhuohui
authors.initials: Z
authors.lastname: Gan
authors.affiliation: Department of Medicine, University of California San Diego, 9500 Gilman Drive, San Diego, CA, 92093-0623, USA.
authors.forename: Zhenxing
authors.initials: Z
authors.lastname: Fu
authors.affiliation: Department of Bioengineering, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, 92093-0412, USA.
authors.forename: Jennifer C
authors.initials: JC
authors.lastname: Stowe
authors.affiliation: Department of Medicine, University of California San Diego, 9500 Gilman Drive, San Diego, CA, 92093-0623, USA.
authors.forename: Frank L
authors.initials: FL
authors.lastname: Powell
authors.affiliation: Department of Bioengineering, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, 92093-0412, USA.
authors.forename: Andrew D
authors.initials: AD
authors.lastname: McCulloch
mesh_terms: D000818:Animals; D019344:Lactic Acid; D055432:Metabolomics / Q000379:methods*; D051379:Mice; D018482:Muscle, Skeletal / Q000378:metabolism*
chemical_list: D019344:Lactic Acid
keywords: Amplex Red; Dissection; Euthanasia; Lactate; Metabolomics; Skeletal muscle