title: [Morphological and molecular characterization of isolates of Macrophomina phaseolina associated with sugarcane in Mexico].
abstract: Charcoal rot caused by Macrophomina phaseolina is an important disease of sugarcane in Mexico. This study was carried out to characterize isolates of M. phaseolina obtained from sugarcane by the combination of morphological and molecular analyses. The morphological characterization of 10 isolates was performed using scanning electron microscopy and light microscopy. To confirm the morphological identification, rDNA from two representative isolates was extracted, and the internal transcribed spacer (ITS) region was amplified by polymerase chain reaction and sequenced using specific primers MpKF1 and MpKR1. Based on their morphological characteristics, all isolates were identified as M. phaseolina. Moreover, the analysis of two ITS sequences showed 100% similarity with the M. phaseolina sequences deposited in the GenBank. To our knowledge, this is the first study in the world aimed at characterizing isolates of M. phaseolina obtained from sugarcane.
authors.affiliation: Departamento de Parasitología Agrícola, Universidad Autónoma Chapingo, Texcoco, Estado de México, México.
authors.forename: Santos G
authors.initials: SG
authors.lastname: Leyva-Mir
authors.affiliation: Departamento de Parasitología Agrícola, Universidad Autónoma Chapingo, Texcoco, Estado de México, México.
authors.forename: Guadalupe C
authors.initials: GC
authors.lastname: Velázquez-Martínez
authors.affiliation: Departamento de Parasitología Agrícola, Universidad Autónoma Chapingo, Texcoco, Estado de México, México.
authors.forename: Bertha
authors.initials: B
authors.lastname: Tlapal-Bolaños
authors.affiliation: Fitopatología, Instituto de Fitosanidad, Colegio de Posgraduados, Texcoco, Estado de México, México. Electronic address: jmtovar@colpos.mx.
authors.forename: Juan M
authors.initials: JM
authors.lastname: Tovar-Pedraza
authors.affiliation: Red de Estudios Moleculares Avanzados, Instituto de Ecología, Xalapa, Veracruz, México.
authors.forename: Greta H
authors.initials: GH
authors.lastname: Rosas-Saito
authors.affiliation: Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de Los Garza, Nuevo León, México.
authors.forename: Omar G
authors.initials: OG
authors.lastname: Alvarado-Gómez
mesh_terms: D001203:Ascomycota / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology / Q000648:ultrastructure; D004271:DNA, Fungal / Q000032:analysis; D021903:DNA, Ribosomal Spacer; D008800:Mexico; D008855:Microscopy, Electron, Scanning; D016533:Mycological Typing Techniques; D010935:Plant Diseases / Q000382:microbiology; D021521:Ribotyping; D031786:Saccharum / Q000382:microbiology*; D013172:Spores, Fungal
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer
keywords: Caracterización morfológica; Macrophomina phaseolina; Microscopia electrónica de barrido; Morphological characterization; Saccharum officinarum; Scanning electron microscopy