title: Imidazolium embedded C8 based stationary phase for simultaneous reversed-phase/hydrophilic interaction mixed-mode chromatography.
abstract: A new imidazolium embedded C8 based stationary phase (SIL-MPS-VOL) was facilely prepared by two steps and characterized by Fourier transform infrared spectrometry and thermogravimetric analysis. Due to the introduction of quaternary imidazolium group to the traditional C8 stationary phase, the developed SIL-MPS-VOL column demonstrated both reversed-phase liquid chromatography (RPLC) and hydrophilic interaction liquid chromatography (HILIC) retention mechanisms. A series of hydrophobic and hydrophilic test samples, including benzene homologues, anilines, positional isomers, nucleosides and nucleotides, were used to evaluate the developed SIL-MPS-VOL stationary phase. A rapid separation time, high separation efficiency and planar selectivity were achieved, compared with the commercially available C8 column. Moreover, the developed stationary phase was further used to detect and separate of melamine in powdered infant formula and high polar component of secondary metabolites of Trichoderma, and improved separation efficiency was achieved, indicating the potential merits of the developed SIL-MPS-VOL stationary phase for simultaneous separation of complex hydrophobic and hydrophilic samples with high selectivity.
authors.affiliation: Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education and Key Laboratory of Pharmaceutical Quality Control of Hebei Province, College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China. Electronic address: xiaoqiao@hbu.edu.cn.
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Qiao
authors.affiliation: Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education and Key Laboratory of Pharmaceutical Quality Control of Hebei Province, College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education and Key Laboratory of Pharmaceutical Quality Control of Hebei Province, College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China.
authors.forename: Niu
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education and Key Laboratory of Pharmaceutical Quality Control of Hebei Province, College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education and Key Laboratory of Pharmaceutical Quality Control of Hebei Province, College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China.
authors.forename: Xinying
authors.initials: X
authors.lastname: Qin
authors.affiliation: Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education and Key Laboratory of Pharmaceutical Quality Control of Hebei Province, College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China.
authors.forename: Hongyuan
authors.initials: H
authors.lastname: Yan
authors.affiliation: Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education and Key Laboratory of Pharmaceutical Quality Control of Hebei Province, College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Liu
mesh_terms: D000814:Aniline Compounds / Q000032:analysis; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002853:Chromatography, Liquid; D056148:Chromatography, Reverse-Phase; D005504:Food Analysis; D057927:Hydrophobic and Hydrophilic Interactions; D009705:Nucleosides / Q000737:chemistry
chemical_list: D000814:Aniline Compounds; D009705:Nucleosides; C023650:aniline
keywords: Hydrophilic interaction liquid chromatography; Imidazolium embedded C(8) stationary phase; Mixed-mode; Reversed-phase liquid chromatography