title: Vascular Mechanics in Decellularized Aortas and Coronary Resistance Microvessels in Type 2 Diabetic db/db Mice.
abstract: We previously reported differences in stiffness between macro- and micro-vessels in type 2 diabetes (T2DM). The aim of this study was to define the mechanical properties of the ECM independent of vascular cells in coronary resistance micro-vessels (CRMs) and macro-vessels (aorta) in control Db/db and T2DM db/db mice. Passive vascular remodeling and mechanics were measured in both intact and decellularized CRMs and aortas from 0 to 125 mmHg. We observed no differences in intact control and diabetic aortic diameters, wall thicknesses, or stiffnesses (p > 0.05). Aortic decellularization caused a significant increase in internal and external diameters and incremental modulus over a range of pressures that occurred to a similar degree in T2DM. Differences in aortic diameters due to decellularization occurred at lower pressures (0-75 mmHg) and converged with intact aortas at higher, physiological pressures (100-125 mmHg). In contrast, CRM decellularization caused increased internal diameter and incremental modulus only in the db/db mice, but unlike the aorta, the intact and decellularized CRM curves were more parallel. These data suggest that (1) micro-vessels may be more sensitive to early adverse consequences of diabetes than macro-vessels and (2) the ECM is a structural limit in aortas, but not CRMs.
authors.affiliation: Department of Biological and Allied Health Sciences, Ohio Northern University College of Arts & Sciences, Ada, OH, USA.
authors.forename: Mircea
authors.initials: M
authors.lastname: Anghelescu
authors.affiliation: Biophysics Graduate Program, Davis Heart and Lung Research Institute, The Ohio State University College of Arts and Sciences, Columbus, OH, USA.
authors.forename: Jeffrey R
authors.initials: JR
authors.lastname: Tonniges
authors.affiliation: Renal Pathology and Electron Microscopy Lab, The Ohio State University Wexner Medical Center, Columbus, OH, USA.
authors.forename: Ed
authors.initials: E
authors.lastname: Calomeni
authors.affiliation: Center for Cardiovascular and Pulmonary Research and The Heart Center, The Research Institute at Nationwide Children's Hospital, Department of Pediatrics, The Ohio State University College of Medicine, 700 Children's Drive, WB4135, Columbus, OH, 43205, USA.
authors.forename: Patricia E
authors.initials: PE
authors.lastname: Shamhart
authors.affiliation: Department of Biomedical Engineering, Davis Heart and Lung Research Institute, The Ohio State University College of Engineering, Columbus, OH, USA.
authors.forename: Gunjan
authors.initials: G
authors.lastname: Agarwal
authors.affiliation: Department of Biomedical Engineering, Davis Heart and Lung Research Institute, The Ohio State University College of Engineering, Columbus, OH, USA.
authors.forename: Keith J
authors.initials: KJ
authors.lastname: Gooch
authors.affiliation: Center for Cardiovascular and Pulmonary Research and The Heart Center, The Research Institute at Nationwide Children's Hospital, Department of Pediatrics, The Ohio State University College of Medicine, 700 Children's Drive, WB4135, Columbus, OH, 43205, USA. aaron.trask@nationwidechildrens.org.
authors.forename: Aaron J
authors.initials: AJ
authors.lastname: Trask
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000502:physiology*; D003326:Coronary Circulation; D003331:Coronary Vessels / Q000502:physiology*; D003924:Diabetes Mellitus, Type 2 / Q000503:physiopathology*; D008297:Male; D051379:Mice; D055806:Microvessels / Q000502:physiology*; D014655:Vascular Resistance
keywords: Extracellular matrix; Pressure myography; Type 2 diabetes; Vascular biomechanics