title: Lack of interference with diagnostic testing for tuberculosis in goats experimentally exposed to Corynebacterium pseudotuberculosis.
abstract: It has been suggested that infection with Corynebacterium pseudotuberculosis, the pathogen responsible for caseous lymphadenitis (CLA), might interfere with diagnostic testing for tuberculosis (TB), due to antigenic similarities between this particular type of bacterium and those expressed by mycobacteria. The aim of the present study was to investigate whether experimental infection with C. pseudotuberculosis in goats impacted on TB testing, using single and comparative intradermal tuberculin (SIT and SCIT respectively) tests and interferon (IFN)-γ assay. No positive reactors were detected among the CLA-affected goats using the SIT/SCIT tests or the interferon IFN-γ assay. A proportion of goats showed inconclusive results to the SIT test and reactions to Mycobacterium avium. There was no evidence that infection with C. pseudotuberculosis interferes with diagnostic testing for TB using standard interpretation of the SIT, SCIT and IFN-γ tests.
authors.affiliation: MAEVA SERVET S.L., C/ de la Fragua 3, Alameda del Valle, Madrid 28749, Spain; Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense, Avda, Puerta de Hierro s/n, Madrid 28040, Spain. Electronic address: jbezosga@visavet.ucm.es.
authors.forename: Javier
authors.initials: J
authors.lastname: Bezos
authors.affiliation: Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense, Avda, Puerta de Hierro s/n, Madrid 28040, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Casal
authors.affiliation: Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense, Avda, Puerta de Hierro s/n, Madrid 28040, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Romero
authors.affiliation: Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense, Avda, Puerta de Hierro s/n, Madrid 28040, Spain.
authors.forename: Emmanouil
authors.initials: E
authors.lastname: Liandris
authors.affiliation: Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense, Avda, Puerta de Hierro s/n, Madrid 28040, Spain.
authors.forename: Natalia
authors.initials: N
authors.lastname: Sánchez
authors.affiliation: Servicio de Sanidad Animal y Laboratorio del Gobierno de Canarias, San Cristóbal de la Laguna 38320, Spain.
authors.forename: Virginia
authors.initials: V
authors.lastname: Vigo
authors.affiliation: Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense, Avda, Puerta de Hierro s/n, Madrid 28040, Spain; Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, Avda. Puerta de Hierro s/n, Madrid 28040, Spain.
authors.forename: Lucas
authors.initials: L
authors.lastname: Domínguez
authors.affiliation: Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense, Avda, Puerta de Hierro s/n, Madrid 28040, Spain; Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, Avda. Puerta de Hierro s/n, Madrid 28040, Spain.
authors.forename: Lucía
authors.initials: L
authors.lastname: de Juan
mesh_terms: D000818:Animals; D003354:Corynebacterium Infections / Q000150:complications / Q000175:diagnosis / Q000662:veterinary*; D016925:Corynebacterium pseudotuberculosis; D003429:Cross Reactions; D006041:Goats; D007371:Interferon-gamma; D012680:Sensitivity and Specificity; D014374:Tuberculin Test / Q000662:veterinary*; D014376:Tuberculosis / Q000150:complications / Q000175:diagnosis / Q000662:veterinary*
chemical_list: D007371:Interferon-gamma
keywords: Corynebacterium pseudotuberculosis; Diagnostic testing; Goat; Tuberculosis