title: Expression of peroxiredoxins and thioredoxins in the mouse spinal cord during embryonic development.
abstract: Reactive oxygen and nitrogen species (ROS/RNS) are natural byproducts of cellular metabolism. Although these molecules are deleterious at high concentrations, moderate levels of ROS/RNS are essential for normal cell function and take part in numerous cellular processes. The regulation of ROS/RNS is largely attended by peroxiredoxins (Prdxs) and their main reductants, thioredoxins (Trxs). Through their oxidoreductase activities, the members of the Trx/Prdx system can also affect certain cellular processes, notably many implicated in central nervous system (CNS) development. Although several studies have investigated the expression of Prdxs and Trxs in mouse, rat, and human adult CNS, few data are available concerning embryonic stages. In this work, we use immunofluorescence analyses to study the distribution of these enzymes during prenatal mouse spinal cord development. Our results highlight several patterns that contrast with available data for the adult. Indeed, Prdx1, Prdx4, and Prdx6, which are expressed in glial cells in the adult CNS, present clear neuronal localization in mouse spinal cord during embryonic development. Additionally, Prdx1, Prdx2, and to a lesser extent Prdx4, Prdx6, and Trx1 are localized mainly in the nucleus of neural cells. Finally, we identified a consistent, intense expression of all Prdxs and Trxs in groups of cells located in ventral regions of the spinal cord that express motor neuronal markers. These striking expression patterns suggest novel functions of these enzymes at these stages and offer clues to the role of the Trx/Prdx system during embryonic development of the spinal cord.
authors.affiliation: Group of Animal Molecular and Cellular Biology, Institut des Sciences de la Vie (ISV), Université catholique de Louvain, 1348, Louvain-la-Neuve, Belgium.
authors.forename: Marc
authors.initials: M
authors.lastname: Pirson
authors.affiliation: Group of Animal Molecular and Cellular Biology, Institut des Sciences de la Vie (ISV), Université catholique de Louvain, 1348, Louvain-la-Neuve, Belgium.
authors.forename: Bernard
authors.initials: B
authors.lastname: Knoops
mesh_terms: D000367:Age Factors; D000818:Animals; D002374:Catalase / Q000378:metabolism; D004622:Embryo, Mammalian; D005260:Female; D051858:Forkhead Transcription Factors / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D006801:Humans; D060850:LIM-Homeodomain Proteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D025261:Mitochondrial Proton-Translocating ATPases / Q000378:metabolism; D009457:Neuroglia / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D054464:Peroxiredoxins / Q000378:metabolism*; D011247:Pregnancy; D051381:Rats; D018000:Receptors, Peptide / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism; D013116:Spinal Cord / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*; D013879:Thioredoxins / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism
chemical_list: C528041:ATPase beta-subunit, rat; D051858:Forkhead Transcription Factors; C432917:Foxp1 protein, mouse; D060850:LIM-Homeodomain Proteins; D018000:Receptors, Peptide; D012097:Repressor Proteins; D014157:Transcription Factors; C063843:insulin gene enhancer binding protein Isl-1; D013879:Thioredoxins; D054464:Peroxiredoxins; D002374:Catalase; D025261:Mitochondrial Proton-Translocating ATPases
keywords: RRID: AB_2107102; RRID: AB_2252786; RRID: AB_2314683; RRID: AB_2315193; RRID: AB_2315195; RRID: AB_2315196; RRID: AB_258720; RRID: AB_301438; antioxidant enzyme; development; immunohistochemistry; motor neuron; spinal cord