title: Screening of a microbial consortium for highly simultaneous degradation of lignocellulose and chlorophenols.
abstract: In this work, spent mushroom substrates were utilized for screening a microbial consortium with highly simultaneous degradation of lignocellulose and chlorophenols. The desired microbial consortium OEM1 was gained through successive cultivation for about 50 generations and its stability of composition was verified by denaturing gradient gel electrophoresis (DGGE) during screening process. It could degrade lignocellulose and chlorophenols at around 50% and 100%, respectively, within 7days. The diversity analysis and the growth characteristics of OEM1 during degradation process were investigated by PCR-DGGE combined with clone and sequence. The results indicated that OEM1 consisted of 31 strains. Proteobacteria and Bacteroidetes were the predominant bacterial groups. The dynamic change of OEM1 illustrated that consortium community structure was effected by pH and substrate alteration and tended to be stable after 6days' cultivation. Furthermore, bacteria (11 strains) and actinomycetes (2 strains) were obtained based on plate isolation and identified via 16S rDNA sequence.
authors.affiliation: College of Natural Resources and Environment, South China Agricultural University, Guangzhou, Guangdong 510642, PR China; State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, PR China.
authors.forename: Jiajin
authors.initials: J
authors.lastname: Liang
authors.affiliation: College of Natural Resources and Environment, South China Agricultural University, Guangzhou, Guangdong 510642, PR China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Peng
authors.affiliation: College of Natural Resources and Environment, South China Agricultural University, Guangzhou, Guangdong 510642, PR China.
authors.forename: Dexing
authors.initials: D
authors.lastname: Yin
authors.affiliation: College of Natural Resources and Environment, South China Agricultural University, Guangzhou, Guangdong 510642, PR China.
authors.forename: Beiyin
authors.initials: B
authors.lastname: Li
authors.affiliation: College of Natural Resources and Environment, South China Agricultural University, Guangzhou, Guangdong 510642, PR China.
authors.forename: Dehan
authors.initials: D
authors.lastname: Wang
authors.affiliation: College of Natural Resources and Environment, South China Agricultural University, Guangzhou, Guangdong 510642, PR China. Electronic address: yqlin@scau.edu.cn.
authors.forename: Yunqin
authors.initials: Y
authors.lastname: Lin
mesh_terms: D000363:Agaricales / Q000302:isolation & purification*; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism*; D015373:Bacterial Typing Techniques; D001673:Biodegradation, Environmental; D002733:Chlorophenols / Q000378:metabolism*; D007220:Industrial Waste / Q000517:prevention & control; D008031:Lignin / Q000378:metabolism*; D059013:Microbial Consortia / Q000502:physiology*; D013045:Species Specificity
chemical_list: D002733:Chlorophenols; D007220:Industrial Waste; C036909:lignocellulose; D008031:Lignin
keywords: Biodegradation; Chlorophenols; Lignocellulose; Microbial consortium; Spent mushroom substrate