title: General approach for electrochemical detection of persistent pharmaceutical micropollutants: Application to acetaminophen.
abstract: We propose in this work a general and versatile methodology for electrochemical monitoring of persistent pharmaceutical micropollutants. The system presented is based on an electroactive and electropolymerized hapten (mimetic molecule of the pollutant to be detected) and a specific antibody that competitively binds either the hapten or the pollutant. The current delivered by the device depends on this competitive equilibrium and therefore on the pollutant's concentration. The determination of the pharmaceutical product operates within minutes, using square wave voltammetry without labeling or addition of a reactant in solution; the competitive hapten/antibody transduction produces a "signal-on" (a current increase). Applied to acetaminophen, this electrochemical immunosensor presents a very low detection limit of ca. 10 pM, (S/N=3) and a very high selectivity towards structural analogs (aspirin, BPA, and piperazine) even in a mixture.
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France.
authors.forename: S
authors.initials: S
authors.lastname: Shi
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France. Electronic address: steeve.reisberg@univ-paris-diderot.fr.
authors.forename: S
authors.initials: S
authors.lastname: Reisberg
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France.
authors.forename: G
authors.initials: G
authors.lastname: Anquetin
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France.
authors.forename: V
authors.initials: V
authors.lastname: Noël
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France.
authors.forename: M C
authors.initials: MC
authors.lastname: Pham
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France. Electronic address: piro@univ-paris-diderot.fr.
authors.forename: B
authors.initials: B
authors.lastname: Piro
mesh_terms: D000082:Acetaminophen / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods; D057930:Click Chemistry; D055664:Electrochemical Techniques / Q000379:methods*; D006241:Haptens / Q000737:chemistry; D007118:Immunoassay / Q000379:methods; D057230:Limit of Detection; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D006241:Haptens; D014874:Water Pollutants, Chemical; D000082:Acetaminophen
keywords: Acetaminophen; Competitive immunoassay; Electrochemical immunosensor; Hapten; Label-free; Reagentless