title: Casein Kinase 1 and Phosphorylation of Cohesin Subunit Rec11 (SA3) Promote Meiotic Recombination through Linear Element Formation.
abstract: Proper meiotic chromosome segregation, essential for sexual reproduction, requires timely formation and removal of sister chromatid cohesion and crossing-over between homologs. Early in meiosis cohesins hold sisters together and also promote formation of DNA double-strand breaks, obligate precursors to crossovers. Later, cohesin cleavage allows chromosome segregation. We show that in fission yeast redundant casein kinase 1 homologs, Hhp1 and Hhp2, previously shown to regulate segregation via phosphorylation of the Rec8 cohesin subunit, are also required for high-level meiotic DNA breakage and recombination. Unexpectedly, these kinases also mediate phosphorylation of a different meiosis-specific cohesin subunit Rec11. This phosphorylation in turn leads to loading of linear element proteins Rec10 and Rec27, related to synaptonemal complex proteins of other species, and thereby promotes DNA breakage and recombination. Our results provide novel insights into the regulation of chromosomal features required for crossing-over and successful reproduction. The mammalian functional homolog of Rec11 (STAG3) is also phosphorylated during meiosis and appears to be required for fertility, indicating wide conservation of the meiotic events reported here.
authors.affiliation: Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America.
authors.forename: Naina
authors.initials: N
authors.lastname: Phadnis
authors.affiliation: Max F. Perutz Laboratories, University of Vienna, Vienna, Austria; Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Slovakia.
authors.forename: Lubos
authors.initials: L
authors.lastname: Cipak
authors.affiliation: Max F. Perutz Laboratories, University of Vienna, Vienna, Austria.
authors.forename: Silvia
authors.initials: S
authors.lastname: Polakova
authors.affiliation: Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America.
authors.forename: Randy W
authors.initials: RW
authors.lastname: Hyppa
authors.affiliation: Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Slovakia.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Cipakova
authors.affiliation: Max F. Perutz Laboratories, University of Vienna, Vienna, Austria.
authors.forename: Dorothea
authors.initials: D
authors.lastname: Anrather
authors.affiliation: Department of Genetics, Comenius University, Bratislava, Slovakia.
authors.forename: Lucia
authors.initials: L
authors.lastname: Karvaiova
authors.affiliation: Research Institute of Molecular Pathology, Vienna, Austria.
authors.forename: Karl
authors.initials: K
authors.lastname: Mechtler
authors.affiliation: Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America.
authors.forename: Gerald R
authors.initials: GR
authors.lastname: Smith
authors.affiliation: Max F. Perutz Laboratories, University of Vienna, Vienna, Austria; Research Institute of Molecular Pathology, Vienna, Austria.
authors.forename: Juraj
authors.initials: J
authors.lastname: Gregan
mesh_terms: D047389:Casein Kinase I / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism; D020090:Chromosome Segregation; D053903:DNA Breaks, Double-Stranded; D004271:DNA, Fungal / Q000235:genetics; D059765:Homologous Recombination; D008540:Meiosis; D009154:Mutation; D010766:Phosphorylation; D011401:Promoter Regions, Genetic; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*; D013573:Synaptonemal Complex / Q000378:metabolism; D000097722:Cohesins
chemical_list: D047389:Casein Kinase I; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D004271:DNA, Fungal; D011494:Protein Kinases; D029702:Schizosaccharomyces pombe Proteins; D000097722:Cohesins; C088232:hhp1 protein, S pombe; C088231:hhp2 protein, S pombe