title: Effects of Cudrania tricuspidata Fruit Extract and Its Active Compound, 5,7,3',4'-Tetrahydroxy-6,8-diprenylisoflavone, on the High-Affinity IgE Receptor-Mediated Activation of Syk in Mast Cells.
abstract: Cudrania tricuspidata fruit extract contains a rich source of prenylated flavonoids with potential antiatherosclerotic, hepatoprotective, and anti-inflammatory properties. However, the effect of C. tricuspidata fruit extracts and its active compounds on the high-affinity IgE receptor (FcεRI)-mediated signaling remains unknown. In the present study, the effect of methanol extract from the fruits of C. tricuspidata (MFC) and its active compound, 5,7,3',4'-tetrahydroxy-6,8-diprenylisoflavone (THDPI), on FcεRI-mediated signaling in mast cells was investigated. MFC and THDPI suppressed mast cell degranulation and Ca(2+) influx. MFC also interfered with IgE-FcεRI interaction and decreased FcεRIβ mRNA expression in mast cells. Furthermore, MFC and THDPI inhibited the phosphorylation of Syk, LAT, and PLCγ and F-actin redistribution. These results indicate that MFC and its active compound, THDPI, inhibit mast cell activation through the inhibition of FcεRI-mediated Syk activation, suggesting a therapeutic potential for controlling mast cell activation in inflammatory and/or allergic processes.
authors.affiliation: †Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Taehun
authors.initials: T
authors.lastname: Lee
authors.affiliation: §Department of Biosystems and Biotechnology, Korea University, Seoul 136-713, Republic of Korea.
authors.forename: Jaeyoung
authors.initials: J
authors.lastname: Kwon
authors.affiliation: §Department of Biosystems and Biotechnology, Korea University, Seoul 136-713, Republic of Korea.
authors.forename: Dongho
authors.initials: D
authors.lastname: Lee
authors.affiliation: †Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Woongchon
authors.initials: W
authors.lastname: Mar
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D005638:Fruit / Q000737:chemistry; D007529:Isoflavones / Q000737:chemistry / Q000494:pharmacology*; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology; D029586:Moraceae / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics / Q000276:immunology*; D017455:Receptors, IgE / Q000276:immunology*
chemical_list: D000893:Anti-Inflammatory Agents; D007529:Isoflavones; D010936:Plant Extracts; D017455:Receptors, IgE; D020794:Receptor Protein-Tyrosine Kinases
keywords: 5,7,3′,4′-tetrahydroxy-6,8-diprenylisoflavone; Cudrania tricuspidata; FcεRI; Syk; mast cell