title: VKORC1 -1639 G>A Polymorphism in Romanian Patients With Deep Vein Thrombosis.
abstract: OBJECTIVE
  The purpose of the research was to study the influence of several genetic factors, especially the -1693 G>A polymorphism of the VKORC1 gene, on the risk of acute unprovoked lower extremity deep vein thrombosis (DVT).
  
  
  METHODS
  The study included 127 patients (median age 63 [53.2; 72] years; 61 [48%] women and 66 [52%] men) who were diagnosed with acute lower extremity DVT and 114 controls (median age 62 [53; 73] years; 64 [56.1%] women and 50 [43.9%] men) without DVT. We recorded data regarding the history of DVT and the presence of varicose veins. We determined the genotypes for factor V Leiden (FVL) mutation, prothrombin G20210A mutation, VKORC1 -1639 G>A mutation, and PAI-1 -675 4G/5G polymorphism.
  
  
  CONCLUSIONS
  Varicose veins were found in 67 (52.8%) patients and 29 (25.4%) controls (P < .001). FVL was present in 29 (22.8%) patients and 10 (8.8%) controls (P = .005). The VKORC1 (-1693 G>A) GG genotype was found in 42 (33.1%) patients and 41 (36%) controls, the GA genotype in 71 (55.9%) patients and 47 (41.2%) controls, and AA genotype in 14 (11%) patients and 26 (22.8%) controls (P = .020). Multivariate analysis showed that the presence of varicose veins, FVL, and VKORC1 -1639 G>A was independently associated with the risk of DVT. The VKORC1 (-1693 G>A) AA genotype was associated with fewer cases of DVT (odds ratio = 0.435; 95% confidence interval 0.205-0.991; P = .031).
authors.affiliation: Department of Pharmacology, Toxicology and Clinical Pharmacology, "Iuliu Haţieganu" University of Medicine and Pharmacy Cluj-Napoca, Cluj-Napoca, Romania stefanvesa@gmail.com.
authors.forename: Ştefan Cristian
authors.initials: ŞC
authors.lastname: Vesa
authors.affiliation: Department of Medical Genetics, "Iuliu Haţieganu" University of Medicine and Pharmacy Cluj-Napoca, Cluj-Napoca, Romania.
authors.forename: Adrian Pavel
authors.initials: AP
authors.lastname: Trifa
authors.affiliation: Fifth Department of Internal Medicine, Municipal Hospital of Cluj-Napoca, "Iuliu Haţieganu" University of Medicine and Pharmacy Cluj-Napoca, Cluj-Napoca, Romania.
authors.forename: Sorin
authors.initials: S
authors.lastname: Crişan
authors.affiliation: Department of Pharmacology, Toxicology and Clinical Pharmacology, "Iuliu Haţieganu" University of Medicine and Pharmacy Cluj-Napoca, Cluj-Napoca, Romania.
authors.forename: Anca Dana
authors.initials: AD
authors.lastname: Buzoianu
mesh_terms: D000368:Aged; D005260:Female; D006801:Humans; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012189:Retrospective Studies; D012307:Risk Factors; D012383:Romania; D019851:Thrombophilia / Q000235:genetics*; D020246:Venous Thrombosis / Q000235:genetics*; D064417:Vitamin K Epoxide Reductases / Q000235:genetics*
chemical_list: C578523:VKORC1 protein, human; D064417:Vitamin K Epoxide Reductases
keywords: clinical thrombophilia; deep venous thrombosis; gene polymorphisms; thrombophilia