title: Regulation Roles of MICA and NKG2D in Human Renal Cancer Cells.
abstract: OBJECTIVE
  Our aim was to investigation the roles of MHC class I chain-related gene A(MICA) and natural killer cell group 2D(NKG2D) in human renal cancer cells.
  
  
  METHODS
  The expression of membrane MICA (mMICA) on renal cells and NKG2D on NK cells were detected by flow cytometry (FCM); the content of sMICA were detected by enzyme linked immunosorbent assay (ELISA) and the distribution of mMICA on renal tumor tissues by immunohistochemistry; the interaction between MICA and NKG2D was observed by antibody closed method.
  
  
  RESULTS
  Our results showed that the expression of mMICA in renal cancer tissues was significantly higher than in controls, where the soluble MICA was not expressed. Cytotoxic activity of NK cells was significantly reduced after exposure to NKG2D and MICA antibodies (P<0.05), and serum containing sMICA can obviously lower the function of NKG2D (P<0.05).
  
  
  CONCLUSIONS
  The interaction of mMICA and NKG2D play important roles in mediation of cytotoxicity of NK cells in RCC. On the other hand, sMICA may mediate tumor immune escape through down- regulated NKG2D expression.
authors.affiliation: Clinical Molecular Biology Laboratory, the Second Hospital of Shandong University, Jinan, Shandong Province, China E-mail : ymlly@qq.com.
authors.forename: Hong-Ying
authors.initials: HY
authors.lastname: Jia
authors.forename: Jun-Li
authors.initials: JL
authors.lastname: Liu
authors.forename: Ming-Zhen
authors.initials: MZ
authors.lastname: Yuan
authors.forename: Cheng-Jun
authors.initials: CJ
authors.lastname: Zhou
authors.forename: Wen-Dong
authors.initials: WD
authors.lastname: Sun
authors.forename: Jing-Jie
authors.initials: JJ
authors.lastname: Zhao
authors.forename: Jue
authors.initials: J
authors.lastname: Wang
authors.forename: Ling
authors.initials: L
authors.lastname: Liu
authors.forename: Yun
authors.initials: Y
authors.lastname: Luan
mesh_terms: D017209:Apoptosis; D002292:Carcinoma, Renal Cell / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D049109:Cell Proliferation; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D015395:Histocompatibility Antigens Class I / Q000378:metabolism*; D006801:Humans; D007680:Kidney Neoplasms / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D007694:Killer Cells, Natural / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D055655:NK Cell Lectin-Like Receptor Subfamily K / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D015395:Histocompatibility Antigens Class I; C527085:KLRK1 protein, human; C099187:MHC class I-related chain A; D055655:NK Cell Lectin-Like Receptor Subfamily K