title: Comparative analysis of the in vitro and in planta secretomes from Mycosphaerella fijiensis isolates.
abstract: Black Sigatoka, a devastating disease of bananas and plantains worldwide, is caused by the fungus Mycosphaerella fijiensis. Several banana cultivars such as 'Yangambi Km 5' and Calcutta IV, have been known to be resistant to the fungus, but the resistance has been broken in 'Yangambi Km 5' in Costa Rica. Since the resistance of this variety still persists in Mexico, the aim of this study was to compare the in vitro and in planta secretomes from two avirulent and virulent M. fijiensis isolates using proteomics and bioinformatics approaches. We aimed to identify differentially expressed proteins in fungal isolates that differ in pathogenicity and that might be responsible for breaking the resistance in 'Yangambi Km 5'. We were able to identify 90 protein spots in the secretomes of fungal isolates encoding 42 unique proteins and 35 differential spots between them. Proteins involved in carbohydrate transport and metabolism were more prevalent. Several proteases, pathogenicity-related, ROS detoxification and unknown proteins were also highly or specifically expressed by the virulent isolate in vitro or during in planta infection. An unknown protein representing a virulence factor candidate was also identified. These results demonstrated that the secretome reflects major differences between both M. fijiensis isolates.
authors.affiliation: Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados del IPN, Unidad Irapuato, 36821, Irapuato, Guanajuato, Mexico.
authors.forename: Lina
authors.initials: L
authors.lastname: Escobar-Tovar
authors.affiliation: Dirección de Investigaciones, Sección de Fitopatología, Corporación Bananera Nacional, 390-7210, La Rita, Guápiles, Costa Rica.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Guzmán-Quesada
authors.affiliation: Dirección de Investigaciones, Sección de Fitopatología, Corporación Bananera Nacional, 390-7210, La Rita, Guápiles, Costa Rica.
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Sandoval-Fernández
authors.affiliation: Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados del IPN, Unidad Irapuato, 36821, Irapuato, Guanajuato, Mexico. Electronic address: mgomez@ira.cinvestav.mx.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Gómez-Lim
mesh_terms: D001203:Ascomycota / Q000302:isolation & purification / Q000378:metabolism* / Q000472:pathogenicity; D003364:Costa Rica; D005656:Fungal Proteins / Q000378:metabolism*; D008800:Mexico; D028521:Musa / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology*; D020543:Proteome / Q000032:analysis*; D037521:Virulence Factors / Q000032:analysis
chemical_list: D005656:Fungal Proteins; D020543:Proteome; D037521:Virulence Factors
keywords: Ascomycete fungus; Black Sigatoka; Pathogen resistance breakdown; Proteomic analysis; Virulence