title: Screening of aptamers specific to colorectal cancer cells and stem cells by utilizing On-chip Cell-SELEX.
abstract: Colorectal cancer (CRC) is the most frequently diagnosed cancer around the world, causing about 700,000 deaths every year. It is clear now that a small fraction of CRC, named colorectal cancer stem cells (CSCs) exhibiting self-renewal and extensive proliferative activities, are hard to be eradicated. Unfortunately, highly specific biomarkers for colorectal CSC (CR-CSCs) are lacking that prohibits the development of effective therapeutic strategies. This study designed and manufactured a novel microfluidic system capable of performing a fully automated cell-based, systematic evolution of ligands by exponential enrichment (SELEX) process. Eight CR-CSC/CRC-specific aptamers were successfully selected using the microfluidic chip. Three of the aptamers showed high affinities towards their respective target cells with a dissociation constant of 27.4, 28.5 and 12.3 nM, which are comparable to that of antibodies.
authors.affiliation: Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan 30013.
authors.forename: Lien-Yu
authors.initials: LY
authors.lastname: Hung
authors.affiliation: Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan 30013.
authors.forename: Chih-Hung
authors.initials: CH
authors.lastname: Wang
authors.affiliation: Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan 30013.
authors.forename: Yu-Jui
authors.initials: YJ
authors.lastname: Che
authors.affiliation: Institute of Molecular Medicine, National Tsing Hua University, Hsinchu, Taiwan 30013.
authors.forename: Chien-Yu
authors.initials: CY
authors.lastname: Fu
authors.affiliation: Institute of Molecular Medicine, National Tsing Hua University, Hsinchu, Taiwan 30013.
authors.forename: Hwan-You
authors.initials: HY
authors.lastname: Chang
authors.affiliation: Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan 11529.
authors.forename: Kuan
authors.initials: K
authors.lastname: Wang
authors.affiliation: 1] Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan 30013 [2] Institute of NanoEngineering and Microsystems, National Tsing Hua University, Hsinchu, Taiwan 30013 [3] Institute of Biomedical Engineering, National Tsing Hua University, Hsinchu, Taiwan 30013.
authors.forename: Gwo-Bin
authors.initials: GB
authors.lastname: Lee
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000378:metabolism*; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000378:metabolism; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000473:pathology; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D018189:Immunomagnetic Separation; D007700:Kinetics; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation; D014411:Neoplastic Stem Cells / Q000166:cytology* / Q000378:metabolism; D052156:SELEX Aptamer Technique
chemical_list: D052157:Aptamers, Nucleotide; D014408:Biomarkers, Tumor; D004277:DNA, Single-Stranded