title: Maternal micronutrient deficiency leads to alteration in the kidney proteome in rat pups.
abstract: Maternal nutritional deficiency significantly perturbs the offspring's physiology predisposing them to metabolic diseases during adulthood. Vitamin B12 and folate are two such micronutrients, whose deficiency leads to elevated homocysteine levels. We earlier generated B12 and/or folate deficient rat models and using high-throughput proteomic approach, showed that maternal vitamin B12 deficiency modulates carbohydrate and lipid metabolism in the liver of pups through regulation of PPAR signaling pathway. In this study, using similar approach, we identified 26 differentially expressed proteins in the kidney of pups born to mothers fed with vitamin B12 deficient diet while only four proteins were identified in the folate deficient group. Importantly, proteins like calreticulin, cofilin 1 and nucleoside diphosphate kinase B that are involved in the functioning of the kidney were upregulated in B12 deficient group. Our results hint towards a larger effect of vitamin B12 deficiency compared to that of folate presumably due to greater elevation of homocysteine in vitamin B12 deficient group. In view of widespread vitamin B12 and folate deficiency and its association with several diseases like anemia, cardiovascular and renal diseases, our results may have large implications for kidney diseases in populations deficient in vitamin B12 especially in vegetarians and the elderly people.This article is part of a Special Issue entitled: Proteomics in India.
authors.affiliation: Genomics and Molecular Medicine unit, CSIR-Institute of Genomics and Integrative Biology, Sukhdev Vihar, Mathura Road, New Delhi 110 020, India.
authors.forename: Shadab
authors.initials: S
authors.lastname: Ahmad
authors.affiliation: Genomics and Molecular Medicine unit, CSIR-Institute of Genomics and Integrative Biology, Sukhdev Vihar, Mathura Road, New Delhi 110 020, India; Academy of Scientific & Innovative Research (AcSIR), CSIR-IGIB Campus, New Delhi, India.
authors.forename: Trayambak
authors.initials: T
authors.lastname: Basak
authors.affiliation: National Institute of Nutrition, Hyderabad, India.
authors.forename: K
authors.initials: K
authors.lastname: Anand Kumar
authors.affiliation: Genomics and Molecular Medicine unit, CSIR-Institute of Genomics and Integrative Biology, Sukhdev Vihar, Mathura Road, New Delhi 110 020, India.
authors.forename: Gourav
authors.initials: G
authors.lastname: Bhardwaj
authors.affiliation: National Institute of Nutrition, Hyderabad, India.
authors.forename: A
authors.initials: A
authors.lastname: Lalitha
authors.affiliation: CSIR-Centre for Cellular and Molecular Biology, Hyderabad, India.
authors.forename: Dilip K
authors.initials: DK
authors.lastname: Yadav
authors.affiliation: CSIR-Centre for Cellular and Molecular Biology, Hyderabad, India.
authors.forename: Giriraj Ratan
authors.initials: GR
authors.lastname: Chandak
authors.affiliation: National Institute of Nutrition, Hyderabad, India.
authors.forename: Manchala
authors.initials: M
authors.lastname: Raghunath
authors.affiliation: Genomics and Molecular Medicine unit, CSIR-Institute of Genomics and Integrative Biology, Sukhdev Vihar, Mathura Road, New Delhi 110 020, India; Academy of Scientific & Innovative Research (AcSIR), CSIR-IGIB Campus, New Delhi, India. Electronic address: shantanus@igib.res.in.
authors.forename: Shantanu
authors.initials: S
authors.lastname: Sengupta
mesh_terms: D000818:Animals; D005260:Female; D007668:Kidney / Q000378:metabolism* / Q000473:pathology; D018811:Maternal Exposure / Q000009:adverse effects*; D018977:Micronutrients / Q000172:deficiency*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000378:metabolism* / Q000473:pathology; D020543:Proteome / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D018977:Micronutrients; D020543:Proteome
keywords: 2D-DIGE; Mass spectrometry; Maternal micronutrient deficiency; Tissue proteomics