title: Effect of linear alkylbenzene sulfonate (LAS) on human intestinal Caco-2 cells at non cytotoxic concentrations.
abstract: Linear alkylbenzene sulfonate (LAS) is a cytotoxic synthetic anionic surfactant widely present in the environment due to its large-scale production and intensive use in the detergency field. In this study, we investigated the effect of LAS (CAS No. 25155-30-0) at non cytotoxic concentrations on human intestinal Caco-2 cells using different in vitro bioassays. As results, LAS increased Caco-2 cell proliferation at concentrations ranging from 1 to 15 ppm, more significantly for shorter exposure time (24 h), confirmed using flow cytometry and trypan blue exclusion methods. Moreover, proteomics analysis revealed that this effect was associated with an over-expression of elongation factor 2 and dipeptidyl peptidase 3, and a down-regulation of 14-3-3 protein theta, confirmed at mRNA level using real-time PCR. These findings suggest that LAS at non cytotoxic concentrations, similar to those observed at wastewater treatment plants outlets, increases the growth rate of colon cancer cells, raising thereby its tumor promotion effect potential.
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki, 305-8572, Japan.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Bradai
authors.affiliation: Faculty of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki, 305-8572, Japan.|Alliance of Research on North Africa, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki, 305-8572, Japan.
authors.forename: Junkyu
authors.initials: J
authors.lastname: Han
authors.affiliation: Faculty of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki, 305-8572, Japan.
authors.forename: Abdelfatteh El
authors.initials: AE
authors.lastname: Omri
authors.affiliation: Graduate School of Engineering, Hokkaido University, Sapporo, 060-0808, Japan.
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Funamizu
authors.affiliation: Laboratory of Environmental Bioprocesses, Biotechnology Center of Sfax, Sfax, PB 3018, Tunisia.
authors.forename: Sami
authors.initials: S
authors.lastname: Sayadi
authors.affiliation: Faculty of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki, 305-8572, Japan. isoda.hiroko.ga@u.tsukuba.ac.jp.|Alliance of Research on North Africa, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki, 305-8572, Japan. isoda.hiroko.ga@u.tsukuba.ac.jp.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Isoda
keywords: 14-3-3 Protein theta; Caco-2 cells; Cell proliferation; Dipeptidyl peptidase 3; Elongation factor-2; Linear alkylbenzene sulfonate; Proteomics