title: Preclinical mouse models of osteosarcoma.
abstract: Osteosarcoma is the most common form of primary bone tumors with high prevalence in children. Survival rates of osteosarcoma are low, especially in the case of metastases. Mouse models of this disease have been very valuable in investigation of mechanisms of tumorigenesis, metastasis, as well as testing possible therapeutic options. In this chapter, we summarize currently available mouse models for osteosarcoma and provide detailed methodology for the isolation of cell lines from genetically engineered mouse models (GEMMs), gene modification and tumor cell injection methods, as well as imaging techniques.
authors.affiliation: BBVA Foundation-CNIO Cancer Cell Biology Programme, Spanish National Cancer Research Centre (CNIO) , Madrid, Spain.
authors.forename: Özge
authors.initials: Ö
authors.lastname: Uluçkan
authors.affiliation: INSERM, UMR957, Equipe LIGUE 2012, Physiopathologie de la Résorption Osseuse et Thérapie des Tumeurs Osseuses Primitives, Faculté de Médecine , Nantes, France.
authors.forename: Aude
authors.initials: A
authors.lastname: Segaliny
authors.affiliation: Laboratory for Orthopedic Research, Department of Orthopedics, Balgrist University Hospital , Zürich, Switzerland.
authors.forename: Sander
authors.initials: S
authors.lastname: Botter
authors.affiliation: Department of Pediatrics, The University of Texas MD Anderson Cancer Center , Houston, TX, USA.
authors.forename: Janice M
authors.initials: JM
authors.lastname: Santiago
authors.affiliation: Department of Clinical Studies, Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph , Guelph, Ontario, Canada.
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Mutsaers