title: Zinc oxide nanoparticles induced cyto- and genotoxicity in kidney epithelial cells.
abstract: The wide uses of zinc oxide nanoparticles (nano-ZnO) in industrial, cosmetics, medicine, food production and electronics associate with increase in occupational and public exposure. Although, toxicity of nano-ZnO has been extensively studied on many different cell types and animal systems, there is a significant lack of toxicological data focus on nephrotoxic potential of nano-ZnO. In this study, the cyto- and genotoxic effects of nano-ZnO on rat kidney epithelial cells (NRK-52E) were investigated by using different assays. Nano-ZnO (10-50 nm of sizes) were synthesized by sol-gel method. For the cytotoxic effect of nano-ZnO, mean of inhibition concentration (IC50) values in cell line was evaluated by MTT, Trypan Blue (TB) and Neutral Red Uptake (NRU) assays at 25.0-100.0 μg/mL exposure concentrations. Nano-ZnO showed cytotoxic activity by acting on different targets in renal cells, with IC50 ≥ 73.05 μg/mL. Comet assay was used to evaluate the genotoxicity of nano-ZnO (12.5-50.0 μg/mL). Nano-ZnO caused statistically significant DNA damage. Our results highlight the important risk of cyto- and genotoxic effects of nano-ZnO over the kidney.
authors.affiliation: a Department of Physics, Faculty of Science and.
authors.forename: Neslihan Kılıç
authors.initials: NK
authors.lastname: Uzar
authors.forename: Mahmut
authors.initials: M
authors.lastname: Abudayyak
authors.forename: Namik
authors.initials: N
authors.lastname: Akcay
authors.forename: Gokhan
authors.initials: G
authors.lastname: Algun
authors.forename: Gül
authors.initials: G
authors.lastname: Özhan
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D020552:Comet Assay; D004249:DNA Damage; D003879:Dermatologic Agents / Q000633:toxicity*; D004847:Epithelial Cells / Q000187:drug effects*; D007668:Kidney / Q000166:cytology / Q000187:drug effects*; D053768:Metal Nanoparticles / Q000633:toxicity*; D009153:Mutagens / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects; D010316:Particle Size; D051381:Rats; D015034:Zinc Oxide / Q000633:toxicity*
chemical_list: D003879:Dermatologic Agents; D009153:Mutagens; D015034:Zinc Oxide
keywords: Cytotoxicity; genotoxicity; nanoparticle; nephrotoxicity; zinc oxide