title: Salvianolic acid B attenuates lung inflammation induced by cigarette smoke in mice.
abstract: Salvianolic acid B (Sal B), a bioactive compound isolated from the Chinese herb Radix Salviae Miltiorrhizae, has been reported to exhibit anti-inflammatory and anti-oxidantive effects. The aim of this study was to investigate the protective effects of Sal B on cigarette smoke (CS)-induced acute lung inflammation. Sal B was given intraperitoneally (i.p.) to mice 1h before CS exposure daily for four consecutive days. Bronchoalveolar lavage fluid (BALF) was collected to assess the levels of inflammatory cytokines and cell counts. Lung tissues were used to analysis pathological changes, total glutathione (GSH), nuclear factor erythroid-2 related factor 2 (Nrf-2), and nuclear factor-kappa B (NF-κB) expression. The results showed that Sal B inhibited CS-induced lung pathological changes, the infiltration of inflammatory cells, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β), and monocyte chemoattractant protein 1 (MCP-1) productions. Sal B also up-regulated CS-induced total glutathione (GSH) production. Furthermore, Sal B was found to up-regulate Nrf-2, hemeoxygenase1 (HO1) expression and suppress CS-induced NF-κB activation. In conclusion, the current study demonstrated that Sal B exhibited a protective effect on CS-induced lung injury and the possible mechanism was involved in activating Nrf-2 and inhibiting NF-κB activation.
authors.affiliation: Departmant of Pharmacy, Liaocheng People's Hospital, Liaocheng 252000, Shandong Province, China. Electronic address: zhangdongfangwb@163.com.
authors.forename: Dong-Fang
authors.initials: DF
authors.lastname: Zhang
authors.affiliation: Departmant of Neuroolgy, Liaocheng People's Hospital, Liaocheng 252000, Shandong Province, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Liaocheng University, Liaocheng 252000, Shangdong Province, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Li
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001572:Benzofurans / Q000494:pharmacology*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000276:immunology; D016207:Cytokines / Q000378:metabolism; D019610:Cytoprotection; D004195:Disease Models, Animal; D005978:Glutathione / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000378:metabolism; D008168:Lung / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D008810:Mice, Inbred C57BL; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D011014:Pneumonia / Q000276:immunology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D012906:Smoke / Q000009:adverse effects*; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*; D053630:Depsides
chemical_list: D000893:Anti-Inflammatory Agents; D001572:Benzofurans; D016207:Cytokines; D005978:Glutathione; D051547:Heme Oxygenase-1; D008565:Membrane Proteins; D051267:NF-E2-Related Factor 2; D016328:NF-kappa B; D012906:Smoke; D014028:Tobacco Smoke Pollution; C495636:Nfe2l2 protein, mouse; C076944:salvianolic acid B; D053630:Depsides; C496542:Hmox1 protein, mouse
keywords: Cigarette smoke; Lung injury; NF-κB; Nrf-2; Salvianolic acid B