title: Transfer of CMY-2 Cephalosporinase from Escherichia coli to Virulent Klebsiella pneumoniae Causing a Recurrent Liver Abscess.
abstract: A CMY-2-producing capsular type K2 Klebsiella pneumoniae strain (TVGHKP93) with multidrug resistance was isolated from a recurrent liver abscess in a patient who also carried a CMY-2-producing Escherichia coli strain (TVGHEC01) in the stool. TVGHKP93 retained its high virulence compared with that of the isogenic strain (TVGHKP60) with wild-type resistance from the first liver abscess. Our conjugation experiment showed the successful transfer of the blaCMY-2-carrying plasmid from TVGHEC01 into TVGHKP60. The transconjugant showed both high virulence and the multidrug-resistant phenotype, as did TVGHKP93.
authors.affiliation: Division of Infectious Diseases, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan School of Medicine, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Yi-Tsung
authors.initials: YT
authors.lastname: Lin
authors.affiliation: Department of Microbiology, National Taiwan University College of Medicine, Taipei, Taiwan.
authors.forename: Yi-Jiun
authors.initials: YJ
authors.lastname: Pan
authors.affiliation: Department of Microbiology, National Taiwan University College of Medicine, Taipei, Taiwan.
authors.forename: Tzu-Lung
authors.initials: TL
authors.lastname: Lin
authors.affiliation: Division of Infectious Diseases, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan School of Medicine, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Chang-Phone
authors.initials: CP
authors.lastname: Fung
authors.affiliation: Department of Microbiology, National Taiwan University College of Medicine, Taipei, Taiwan Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan wangjt@ntu.edu.tw.
authors.forename: Jin-Town
authors.initials: JT
authors.lastname: Wang
mesh_terms: D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D004927:Escherichia coli Infections / Q000201:enzymology / Q000235:genetics / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D006801:Humans; D007710:Klebsiella Infections / Q000201:enzymology / Q000235:genetics / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000235:genetics*; D008100:Liver Abscess / Q000201:enzymology* / Q000235:genetics* / Q000382:microbiology; D010957:Plasmids / Q000235:genetics; D014774:Virulence / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: C103022:beta-lactamase CMY-2; D001618:beta-Lactamases