title: Enantioselective analysis of D- and L-amino acids from mouse macrophages using high performance liquid chromatography.
abstract: The intrinsic D-amino acid profile of mouse macrophages extracted from the peritoneal cavity was analyzed using high performance liquid chromatography. Six D-amino acids (D-Asp, D-Ser, D-Ala, D-Leu, D-Gln and D-Lys) were detected in cell lysates of mouse macrophages. The content and the D/D+L ratio differed depending on the type of D-amino acid and were approximately 3.5-22 nmol/g cells, and approximately 1-20%, respectively. The D-amino acid composition of RAW 264.7 cells, which is a model macrophage cell line, was similar to that of the mouse macrophage. These results suggest that macrophages and RAW 264.7 cells with macrophage-like functions have a similar D-amino acid profile.
authors.affiliation: High Technology Research Core, Kansai University, 3-3-35 Yamate-cho, Suita, Osaka 564-8680, Japan.
authors.forename: Shiro
authors.initials: S
authors.lastname: Kato
authors.affiliation: Department of Microbiological Chemistry, Kobe Pharmaceutical University, 4-9-1 Motoyamakita-machi, Higashinada-ku, Kobe, Hyougo 658-8558, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Masuda
authors.affiliation: Department of Microbiological Chemistry, Kobe Pharmaceutical University, 4-9-1 Motoyamakita-machi, Higashinada-ku, Kobe, Hyougo 658-8558, Japan.
authors.forename: Morichika
authors.initials: M
authors.lastname: Konishi
authors.affiliation: High Technology Research Core, Kansai University, 3-3-35 Yamate-cho, Suita, Osaka 564-8680, Japan; Faculty of Chemistry, Materials, and Bioengineering, Kansai University, 3-3-35 Yamate-cho, Suita, Osaka 564-8680, Japan. Electronic address: oikawa@kansai-u.ac.jp.
authors.forename: Tadao
authors.initials: T
authors.lastname: Oikawa
mesh_terms: D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008264:Macrophages / Q000737:chemistry* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D013237:Stereoisomerism
chemical_list: D000596:Amino Acids
keywords: Enantioselective analysis; Immune cell; Macrophage; RAW 264.7 cell; d-Amino acids