title: High Modulus Biodegradable Polyurethanes for Vascular Stents: Evaluation of Accelerated in vitro Degradation and Cell Viability of Degradation Products.
abstract: We have recently reported the mechanical properties and hydrolytic degradation behavior of a series of NovoSorb™ biodegradable polyurethanes (PUs) prepared by varying the hard segment (HS) weight percentage from 60 to 100. In this study, the in vitro degradation behavior of these PUs with and without extracellular matrix (ECM) coating was investigated under accelerated hydrolytic degradation (phosphate buffer saline; PBS/70°C) conditions. The mass loss at different time intervals and the effect of aqueous degradation products on the viability and growth of human umbilical vein endothelial cells (HUVEC) were examined. The results showed that PUs with HS 80% and below completely disintegrated leaving no visual polymer residue at 18 weeks and the degradation medium turned acidic due to the accumulation of products from the soft segment (SS) degradation. As expected the PU with the lowest HS was the fastest to degrade. The accumulated degradation products, when tested undiluted, showed viability of about 40% for HUVEC cells. However, the viability was over 80% when the solution was diluted to 50% and below. The growth of HUVEC cells is similar to but not identical to that observed with tissue culture polystyrene standard (TCPS). The results from this in vitro study suggested that the PUs in the series degraded primarily due to the SS degradation and the cell viability of the accumulated acidic degradation products showed poor viability to HUVEC cells when tested undiluted, however particles released to the degradation medium showed cell viability over 80%.
authors.affiliation: Faculty of Life and Social Sciences, Swinburne University of Technology , Hawthorn, VIC , Australia ; UMR CNRS 7338 Biomécanique et Bioingénierie, Centre de Recherches de Royallieu, Université de Technologie de Compiègne , Compiègne , France.
authors.forename: Melissa
authors.initials: M
authors.lastname: Sgarioto
authors.affiliation: CSIRO Manufacturing Flagship , Clayton, VIC , Australia.
authors.forename: Raju
authors.initials: R
authors.lastname: Adhikari
authors.affiliation: CSIRO Manufacturing Flagship , Clayton, VIC , Australia.
authors.forename: Pathiraja A
authors.initials: PA
authors.lastname: Gunatillake
authors.affiliation: PolyNovo Biomaterials Pty Ltd. , Port Melbourne, VIC , Australia.
authors.forename: Tim
authors.initials: T
authors.lastname: Moore
authors.affiliation: Faculty of Life and Social Sciences, Swinburne University of Technology , Hawthorn, VIC , Australia.
authors.forename: John
authors.initials: J
authors.lastname: Patterson
authors.affiliation: UMR CNRS 7338 Biomécanique et Bioingénierie, Centre de Recherches de Royallieu, Université de Technologie de Compiègne , Compiègne , France.
authors.forename: Marie-Danielle
authors.initials: MD
authors.lastname: Nagel
authors.affiliation: Faculty of Life and Social Sciences, Swinburne University of Technology , Hawthorn, VIC , Australia.
authors.forename: François
authors.initials: F
authors.lastname: Malherbe
keywords: cardiovascular stents; cytotoxicity; degradation; polyurethane; properties