title: [Establishment of a new human glioma cell line and analysis of its biological characteristics].
abstract: OBJECTIVE
  To establish a new glioma cell line and analyze its biological characteristics, and to provide a useful cellular tool with new features for cancer research.
  
  
  METHODS
  Glioma tissue was taken from surgical specimen clinical of a clinical patient. Primary culture was carried out, and a cell line (SHG139) was established after 10 passages. Immunofluorescence staining was performed to detect the expression of proteins, and cell proliferation and cycle were detected by flow cytometry method (FCM). The biological characteristics of SHG139 cells were detected by chromosome karyotype analysis. SHG139s glioma cells derived from SHG139 glioma cell line were cultured with neural stem cell medium. Then stem cell markers were determined. SHG139s cells were induced with serum-containing medium, and their expression of A2B5, GFAP, β-III tubulin, and GalC was detected. Intracranial xenograft tumor of both SHG139 glioma cells and SHG139s glioma stem cell spheres was generated in rats.
  
  
  RESULTS
  The expressions of A2B5, GalC, GFAP, S-100, and vimentin in the 20 and 60 passages of SHG139 cells were positive, consistent with the immunohistochemical results and pathological features. SHG139 cells proliferated significantly within 24 h after subculture, and their total number of chromosomes was 68 and mostly multiploid. They were positive for A2B5 (84.12±9.96)%, nestin (73.86±5.01)%, and NG2 (73.37±2.09)%. SHG139s cells were induced, and the ratio of positive cells of GFAP, β-III tubulin and GalC was (92.89±2.24)%, (64.85±4.09)% and (33.57±4.14)%, respectively.
  
  
  CONCLUSIONS
  SHG139 is an astroglioma cell line, from which SHG139s cells can be successfully obtained by culture with NSCM. SHG139s cells are of A2B5(+)/CD133(-) GSCs subgroup cells, with potentials of self-renewal and multi-directional differentiation. Compared with the intracranial SHG139 xenograft tumor, the intracranial SHG139s xenograft tumor is more malignant and aggressive.
authors.affiliation: Neurosurgery & Brain and Nerve Research Laboratory, the First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Guilin
authors.initials: G
authors.lastname: Chen
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Li
authors.forename: Xueshun
authors.initials: X
authors.lastname: Xie
authors.forename: Jinming
authors.initials: J
authors.lastname: Chen
authors.forename: Tingfeng
authors.initials: T
authors.lastname: Wu
authors.forename: Xuetao
authors.initials: X
authors.lastname: Li
authors.forename: Hangzhou
authors.initials: H
authors.lastname: Wang
authors.affiliation: Email: zhouyxyq2008@sohu.com.
authors.forename: Youxin
authors.initials: Y
authors.lastname: Zhou
authors.forename: Ziwei
authors.initials: Z
authors.lastname: Du
mesh_terms: D000818:Animals; D001254:Astrocytoma; D001932:Brain Neoplasms; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005434:Flow Cytometry; D005910:Glioma; D006801:Humans; D014411:Neoplastic Stem Cells; D064231:Nestin; D051381:Rats; D014183:Transplantation, Heterologous; D014746:Vimentin
chemical_list: D064231:Nestin; D014746:Vimentin