title: [Determination of aniline in whole blood by using solid-phase extraction and high performance liquid chromatography with fluorescence detector].
abstract: OBJECTIVE
  To establish a method for the determination of trace aniline in whole blood by using solid-phase extraction and high performance liquid chromatography with fluorescence detector.
  
  
  METHODS
  After the whole blood was diluted by water, extracted by acetonitrile, cleaned and enriched by Cleanert NH2 solid-phase extraction (SPE) cartridge, separation was performed on a Kromasil C8 column(250 mm x 4. 6 mm i. d., 5 λm) with the mobile phase of water/acetonitrile (20/80, V/V). Detection was carried out by fluorescence detector at ex 225 nm and λem 335 nm.
  
  
  RESULTS
  Calibration curve was linear in the range of 3. 0 - 200.0 µg/L with a correlation coefficient of 0. 9992, and the limit of quantitation(LOQ) was 3. 0 µg/L. The extraction recoveries were 87. 5% - 104. 4%, and the intra-day and inter-day RSDs were 3. 1% - 6. 6% and 6. 4% - 8. 6%, respectively.
  
  
  CONCLUSIONS
  The developed method is simple, fast, little interference, good specificity for the satisfactory determination of trace aniline in whole blood.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Chen
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Zhao
authors.forename: Micong
authors.initials: M
authors.lastname: Jin
mesh_terms: D000814:Aniline Compounds / Q000097:blood*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005453:Fluorescence; D007202:Indicators and Reagents; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D000814:Aniline Compounds; D007202:Indicators and Reagents; C023650:aniline