title: [Preparation and identification of monoclonal antibodies against infectious bursal disease virus (IBDV) VP4].
abstract: Infectious bursal disease virus (IBDV) VP4 plays an important role in immunosuppression of host. In order to develop monoclonal antibodies (McAbs) against VP4, we vaccinated BALB/c mice with His-VP4, screened and subcloned positive clones. We established 4 hybridoma cell lines that stably secreted McAbs against VP4 and named these cell lines 3B3, 3H11, 4C8 and 4G6, respectively. We tested the dissociation constant (Kd) of these McAbs, and found that their K(d)s were 4.61 x 10(-11), 1.71 x 10(-10), 4.26 x 10(-11), 5.02 x 10(-11), respectively. The isotypes of these McAbs were determined to be IgG1, IgG1, IgG2b and IgG1. These McAbs specifically bound to VP4 in IBDV infected DF-1 cells as demonstrated by Western blotting analysis and fluorescence antibody assay. These McAbs would help to detect IBDV infection and to analyze the biological activities of IBDV VP4.
authors.forename: Ya'nan
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiaying
authors.initials: X
authors.lastname: Li
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Li
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiaoqi
authors.initials: X
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Cao
authors.forename: Shijun J
authors.initials: SJ
authors.lastname: Zheng
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D015153:Blotting, Western; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D006825:Hybridomas; D007243:Infectious bursal disease virus; D051379:Mice; D008807:Mice, Inbred BALB C; D015678:Viral Structural Proteins / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; C478245:VP4 protein, infectious bursal disease virus; D015678:Viral Structural Proteins