title: Development of microsatellite primers in the protected species Viola elatior (Violaceae) using next-generation sequencing.
abstract: OBJECTIVE
  Viola elatior (Violaceae) is a Eurasian perennial plant species in which French populations are threatened by anthropogenic pressures. Microsatellite primers were developed to investigate its genetic structure and diversity.
  
  
  RESULTS
  Eight microsatellite markers were isolated using next-generation sequencing. Loci were amplified and screened for 138 individuals in 17 populations from France. Two of the eight polymorphic loci presented no variability across populations. The total number of alleles per locus varied from two to four. Observed heterozygosity ranged from 0.051 to 1.000. All primers amplified successfully in the closely related species V. pumila.
  
  
  CONCLUSIONS
  This set of microsatellites offers a valuable tool for assessing population genetic diversity of the species to improve its conservation and base management efforts. High observed heterozygosity values probably reflect the particular mating system of the species and suggest an important tendency to clonality.
authors.affiliation: Conservatoire Botanique National du Bassin Parisien, UMS 2699 Inventaire et Suivi de la Biodiversité, Muséum National d'Histoire Naturelle, 61 Rue Buffon, 75005 Paris, France.
authors.forename: Mélina
authors.initials: M
authors.lastname: Celik
authors.affiliation: Conservatoire Botanique National du Bassin Parisien, UMS 2699 Inventaire et Suivi de la Biodiversité, Muséum National d'Histoire Naturelle, 61 Rue Buffon, 75005 Paris, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Wegnez
authors.affiliation: Conservatoire Botanique National du Bassin Parisien, UMS 2699 Inventaire et Suivi de la Biodiversité, Muséum National d'Histoire Naturelle, 61 Rue Buffon, 75005 Paris, France.
authors.forename: Chantal
authors.initials: C
authors.lastname: Griveau
authors.affiliation: Service de Systématique Moléculaire, UMS 2700 CNRS-MNHN, CP26, 57 Rue Cuvier, 75231 Paris Cedex 05, France.
authors.forename: Josie
authors.initials: J
authors.lastname: Lambourdière
authors.affiliation: Service de Systématique Moléculaire, UMS 2700 CNRS-MNHN, CP26, 57 Rue Cuvier, 75231 Paris Cedex 05, France.
authors.forename: Jose
authors.initials: J
authors.lastname: Utge
authors.affiliation: Muséum National d'Histoire Naturelle, Département d'Ecologie et gestion de la Biodiversité, UMR 7204 CESCO, Paris, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Noël
authors.affiliation: Service de Systématique Moléculaire, UMS 2700 CNRS-MNHN, CP26, 57 Rue Cuvier, 75231 Paris Cedex 05, France.
authors.forename: Jawad
authors.initials: J
authors.lastname: Abdelkrim
authors.affiliation: Muséum National d'Histoire Naturelle, Département d'Ecologie et gestion de la Biodiversité, UMR 7204 CESCO, Paris, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Machon
keywords: Viola elatior; Violaceae; conservation; microsatellites; next-generation sequencing; population genetics