title: How perifosine affects liposome-encapsulated drug delivery across a cell barrier.
abstract: BACKGROUND
  The development of efficient drug delivery systems to transport therapeutics across barrier-forming cells remains a challenge. Recently it was shown that liposomes containing perifosine, a synthetic analog of lysophosphatidylcholine, efficiently deliver liposome encapsulated content across barrier-forming cells.
  
  
  METHODS
  To elucidate the mechanism of the delivery, fluorescent and spin labeled analog of perifosine were synthesized and their transport from liposomes to the barrier-forming MDKC cells was measured.
  
  
  CONCLUSIONS
  Perifosine analogs are rapidly transported from liposomes into cell membranes. The total amount of perifosine accumulated in plasma membranes seems to be the most important factor in efficient transepithelial transport of liposome-encapsulated substances. Lysolipid-containing liposomal formulations seem to be promising candidates as drug delivery systems in general.
authors.affiliation: Jožef Stefan Institute, Jamova cesta 39, 1000 Ljubljana, Slovenia.
authors.forename: Anze
authors.initials: A
authors.lastname: Testen
authors.forename: Rok
authors.initials: R
authors.lastname: Podlipec
authors.forename: Janez
authors.initials: J
authors.lastname: Mravljak
authors.forename: Andrea
authors.initials: A
authors.lastname: Orthmann
authors.forename: Marjeta
authors.initials: M
authors.lastname: Šentjurc
authors.forename: Reiner
authors.initials: R
authors.lastname: Zeisig
authors.forename: Janez
authors.initials: J
authors.lastname: Štrancar
authors.forename: Tilen
authors.initials: T
authors.lastname: Koklic
mesh_terms: D001692:Biological Transport / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D002626:Chemistry, Pharmaceutical; D016503:Drug Delivery Systems / Q000379:methods*; D065546:Drug Liberation; D005452:Fluoresceins / Q000737:chemistry; D006801:Humans; D008081:Liposomes / Q000737:chemistry*; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology
chemical_list: D005452:Fluoresceins; D008081:Liposomes; D010767:Phosphorylcholine; C105905:perifosine; C007740:fluorexon