title: [Physico-chemical methods for studing β-amyloid aggregation].
abstract: Alzheimer's disease is the most prevalent neurodegenerative pathology. According to the amyloid cascade hypothesis, a key event of the Alzheimer's disease pathogenesis is a transition of the β-amyloid peptide (Аβ) from the monomeric form to the aggregated state. The mechanism of Аβ aggregation is intensively studied in vitro, by means of synthetic peptides and various physico-chemical methods allowing evaluation of size, molecular structure, and morphology of the formed aggregates. The paper reviews both the well-known and recently introduced physico-chemical methods for analysis of Аβ aggregation, including microscopу, optical and fluorescent methods, method of electron paramagnetic resonance, electrochemical and electrophoretic methods, gel-filtration, and mass spectrometric methods. Merits and drawbacks of the methods are discussed. The unique possibility to simultaneously observe Аβ monomers as well oligomers and large aggregates by means of atomic force microscopy or fluorescence correlation spectroscopy is emphasized. The high detection sensitivity of the latter method, monitoring the aggregation process in Аβ solutions at low peptide concentrations is underlined. Among mass spectrometric methods, the ion mobility mass spectrometry is marked out as a method enabling to obtain information about both the spectrum of Аβ oligomers and their structure. It is pointed out that the use of several methods giving the complementary data about Аβ aggregates is the best experimental approach to studying the process of b-amyloid peptide aggregation in vitro.
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia; Engelhardt Institute of Molecular Biology, Moscow, Russia.
authors.forename: S P
authors.initials: SP
authors.lastname: Radko
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: S A
authors.initials: SA
authors.lastname: Khmeleva
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Suprun
authors.affiliation: Engelhardt Institute of Molecular Biology, Moscow, Russia.
authors.forename: S A
authors.initials: SA
authors.lastname: Kozin
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Bodoev
authors.affiliation: Engelhardt Institute of Molecular Biology, Moscow, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Makarov
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: A I
authors.initials: AI
authors.lastname: Archakov
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Shumyantseva
mesh_terms: D000544:Alzheimer Disease / Q000473:pathology; D016229:Amyloid beta-Peptides / Q000032:analysis* / Q000378:metabolism*; D052160:Benzothiazoles; D003224:Congo Red; D055664:Electrochemical Techniques / Q000379:methods*; D004578:Electron Spin Resonance Spectroscopy; D004586:Electrophoresis / Q000379:methods; D005456:Fluorescent Dyes; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D008853:Microscopy / Q000379:methods*; D018625:Microscopy, Atomic Force; D013050:Spectrometry, Fluorescence; D017550:Spectroscopy, Fourier Transform Infrared; D013844:Thiazoles
chemical_list: D016229:Amyloid beta-Peptides; D052160:Benzothiazoles; D005456:Fluorescent Dyes; D013844:Thiazoles; C009462:thioflavin T; D003224:Congo Red
keywords: Alzheimer's disease; aggregation; analytical methods; b-amyloid