title: Rotavirus.
abstract: Group A rotavirus (RVA) is the major cause of acute gastroenteritis (AGE) in young children worldwide. Introduction of two live, attenuated rotavirus vaccines, Rotarix® and RotaTeq®, has dramatically reduced RVA-associated AGE and mortality. High-throughput, sensitive and specific techniques are required to rapidly diagnose and characterize rotavirus strains in stool samples for proper patient treatment and to monitor circulating vaccine and wild-type rotavirus strains. New molecular assays are rapidly developed that are more sensitive and specific than the conventional assays for detection, genotyping and full genome characterization of circulating rotavirus wild-type and vaccine (Rotarix® and RotaTeq®) strains causing AGE.
authors.affiliation: Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention, 1600 Clifton Road, Northeast, Mail Stop G04, Atlanta, GA 30333, USA.
authors.forename: Mathew D
authors.initials: MD
authors.lastname: Esona
authors.affiliation: Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention, 1600 Clifton Road, Northeast, Mail Stop G04, Atlanta, GA 30333, USA. Electronic address: ijs0@cdc.gov.
authors.forename: Rashi
authors.initials: R
authors.lastname: Gautam
mesh_terms: D000372:Agglutination Tests; D005759:Gastroenteritis / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D060005:Genotyping Techniques; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D007124:Immunoenzyme Techniques; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification / Q000302:isolation & purification* / Q000502:physiology; D012400:Rotavirus Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D017423:Sequence Analysis, RNA; D014776:Virus Cultivation / Q000379:methods
keywords: Acute gastroenteritis; Multiplex assays; Next-generation sequencing; RotaTeq®; Rotarix®; Rotavirus; Rotavirus vaccines; qRT-PCR