title: Neurological morphofunctional differentiation induced by REAC technology in PC12. A neuro protective model for Parkinson's disease.
abstract: Research for the use of physical means, in order to induce cell differentiation for new therapeutic strategies, is one of the most interesting challenges in the field of regenerative medicine, and then in the treatment of neurodegenerative diseases, Parkinson's disease (PD) included. The aim of this work is to verify the effect of the radio electric asymmetric conveyer (REAC) technology on the PC12 rat adrenal pheochromocytoma cell line, as they display metabolic features of PD. PC12 cells were cultured with a REAC regenerative tissue optimization treatment (TO-RGN) for a period ranging between 24 and 192 hours. Gene expression analysis of specific neurogenic genes, as neurogenin-1, beta3-tubulin and Nerve growth factor, together with the immunostaining analysis of the specific neuronal protein beta3-tubulin and tyrosine hydroxylase, shows that the number of cells committed toward the neurogenic phenotype was significantly higher in REAC treated cultures, as compared to control untreated cells. Moreover, MTT and Trypan blue proliferation assays highlighted that cell proliferation was significantly reduced in REAC TO-RGN treated cells. These results open new perspectives in neurodegenerative diseases treatment, particularly in PD. Further studies will be needed to better address the therapeutic potential of the REAC technology.
authors.affiliation: 1] Department of Biomedical Sciences, University of Sassari, 07100, Sassari, Italy [2] Laboratory of Molecular Biology and Stem Cell Engineering - National Institute of Biostructures and Biosystems, 40121, Bologna, Italy [3] Department of Regenerative Medicine, Rinaldi Fontani Institute, 50144, Florence, Italy.
authors.forename: Margherita
authors.initials: M
authors.lastname: Maioli
authors.affiliation: 1] Department of Regenerative Medicine, Rinaldi Fontani Institute, 50144, Florence, Italy [2] Research Department, Rinaldi Fontani Foundation - NPO, 50144, Florence, Italy.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Rinaldi
authors.affiliation: Department of Clinical and Experimental Medicine, University of Sassari, 07100, Sassari, Italy.
authors.forename: Rossana
authors.initials: R
authors.lastname: Migheli
authors.affiliation: 1] Department of Biomedical Sciences, University of Sassari, 07100, Sassari, Italy [2] Laboratory of Molecular Biology and Stem Cell Engineering - National Institute of Biostructures and Biosystems, 40121, Bologna, Italy.
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Pigliaru
authors.affiliation: Department of Clinical and Experimental Medicine, University of Sassari, 07100, Sassari, Italy.
authors.forename: Gaia
authors.initials: G
authors.lastname: Rocchitta
authors.affiliation: 1] Department of Biomedical Sciences, University of Sassari, 07100, Sassari, Italy [2] Laboratory of Molecular Biology and Stem Cell Engineering - National Institute of Biostructures and Biosystems, 40121, Bologna, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Santaniello
authors.affiliation: Department of Biomedical Sciences, University of Sassari, 07100, Sassari, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Basoli
authors.affiliation: 1] Department of Regenerative Medicine, Rinaldi Fontani Institute, 50144, Florence, Italy [2] Research Department, Rinaldi Fontani Foundation - NPO, 50144, Florence, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Castagna
authors.affiliation: 1] Department of Regenerative Medicine, Rinaldi Fontani Institute, 50144, Florence, Italy [2] Research Department, Rinaldi Fontani Foundation - NPO, 50144, Florence, Italy.
authors.forename: Vania
authors.initials: V
authors.lastname: Fontani
authors.affiliation: 1] Laboratory of Molecular Biology and Stem Cell Engineering - National Institute of Biostructures and Biosystems, 40121, Bologna, Italy [2] Stem Wave Institute for Tissue Healing (SWITH), Gruppo Villa Maria and Ettore Sansavini Health Science Foundation NPO, 48022, Lugo, Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Ventura
authors.affiliation: Department of Clinical and Experimental Medicine, University of Sassari, 07100, Sassari, Italy.
authors.forename: Pier Andrea
authors.initials: PA
authors.lastname: Serra
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000096:biosynthesis / Q000235:genetics; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D046690:Deep Brain Stimulation / Q000379:methods*; D059290:Dopaminergic Neurons / Q000166:cytology*; D004599:Electric Stimulation Therapy / Q000379:methods*; D020869:Gene Expression Profiling; D020932:Nerve Growth Factor / Q000096:biosynthesis / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics; D000066829:Neuroprotection / Q000502:physiology*; D016716:PC12 Cells; D010300:Parkinson Disease / Q000628:therapy*; D051381:Rats; D014404:Tubulin / Q000096:biosynthesis / Q000235:genetics; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D020932:Nerve Growth Factor; D009419:Nerve Tissue Proteins; D014404:Tubulin; D014446:Tyrosine 3-Monooxygenase; C510879:neurogenin-1, rat