title: ICSH guidelines for the standardization of bone marrow immunohistochemistry.
abstract: Bone marrow (BM) tissue biopsy evaluation, including trephine biopsy and clot section, is an integral part of BM investigation and is often followed by ancillary studies, in particular immunohistochemistry (IHC). IHC provides in situ coupling of morphological assessment and immunophenotype. The number of different IHC tests that can be applied to BM trephine biopsies and the number of indications for IHC testing is increasing concurrently with the development of flow cytometry and molecular diagnostic methods. An international Working Party for the Standardization of Bone Marrow IHC was formed by the International Council for Standardization in Hematology (ICSH) to prepare a set of guidelines for the standardization of BM IHC based on currently available published evidence and modern understanding of quality assurance principles as applied to IHC in general. The guidelines were discussed at the ICSH General Assemblies and reviewed by an international panel of experts to achieve further consensus and represent further development of the previously published ICSH guidelines for the standardization of BM specimens handling and reports.
authors.affiliation: Department of Laboratory Hematology, University Health Network, University of Toronto, Toronto, ON, Canada.
authors.forename: E E
authors.initials: EE
authors.lastname: Torlakovic
authors.affiliation: Department of Pathology, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
authors.forename: R K
authors.initials: RK
authors.lastname: Brynes
authors.affiliation: Department of Pathology, University of Chicago, Chicago, IL, USA.
authors.forename: E
authors.initials: E
authors.lastname: Hyjek
authors.affiliation: Department of Haematology, St George Hospital, SEALS Central, Sydney, NSW, Australia.
authors.forename: S-H
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Pathology, Hannover Medical School, Hannover, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Kreipe
authors.affiliation: Munich Municipal Hospital, Institute of Pathology, Munich, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Kremer
authors.affiliation: Special Hematology, Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN, USA.
authors.forename: R
authors.initials: R
authors.lastname: McKenna
authors.affiliation: Department of Pathology, Kawasaki Medical School, Kurashiki, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Sadahira
authors.affiliation: Institute of Pathology, University Hospital Basel, Basel, Switzerland.
authors.forename: A
authors.initials: A
authors.lastname: Tzankov
authors.affiliation: Department of Clinical Pathology, Sunnybrook Health Sciences Centre, Toronto, ON, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Reis
authors.affiliation: Department of Laboratory Hematology, University Health Network, University of Toronto, Toronto, ON, Canada.|Department of Pathology, Karolinska Institute, Stockholm, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Porwit
mesh_terms: D001706:Biopsy / Q000592:standards; D001853:Bone Marrow / Q000473:pathology* / Q000601:surgery; D001856:Bone Marrow Examination / Q000592:standards*; D003648:Decalcification Technique / Q000592:standards; D005434:Flow Cytometry / Q000592:standards*; D006801:Humans; D007150:Immunohistochemistry / Q000592:standards*; D016130:Immunophenotyping / Q000592:standards*; D007391:International Cooperation; D059021:Laboratory Proficiency Testing; D016612:Paraffin Embedding / Q000592:standards; D011786:Quality Control; D016707:Tissue Fixation / Q000592:standards
keywords: Standardization; bone marrow; immunohistochemistry; trephine biopsy