title: Molecular cloning and expression analysis of a Cu/Zn SOD gene (BcCSD1) from Brassica campestris ssp. chinensis.
abstract: Superoxide dismutases (SODs) are a family of metalloproteins extensively exists in eukaryote, which plays an essential role in stress-tolerance of higher plants. A full-length cDNA encoding Cu/Zn SOD (BcCSD1) was isolated from young seedlings of non-heading Chinese cabbage (Brassica campestris ssp. chinensis) by rapid amplification of cDNA ends (RACE). Bioinformatics analysis revealed that BcCSD1 belonged to the plant SOD super family and had the closest relationship with SOD from Brassica napus. Tissue expression pattern analysis revealed that the BcCSD1 was constitutively expressed in all the tested tissues, and strongest in leaf, moderate in stem, lowest in root. The expression profiles under different stress treatments such as drought, NaCl, high temperature and ABA were also investigated, and the results revealed that BcCSD1 was a stress-responsive gene, especially to ABA. These results provide useful information for further understanding the role of BcCSD1 resistant to abiotic stress in Brassica campestris in the future.
authors.affiliation: Laboratory of Plant Biotechnology, Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, PR China.
authors.forename: Lijie
authors.initials: L
authors.lastname: Cui
authors.affiliation: Laboratory of Plant Biotechnology, Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, PR China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Laboratory of Plant Biotechnology, Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Yan
authors.affiliation: Laboratory of Plant Biotechnology, Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, PR China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Laboratory of Plant Biotechnology, Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, PR China.
authors.forename: Zhongyin
authors.initials: Z
authors.lastname: Qian
authors.affiliation: Laboratory of Plant Biotechnology, Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, PR China.
authors.forename: Jingxian
authors.initials: J
authors.lastname: Pan
authors.affiliation: Laboratory of Plant Biotechnology, Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, PR China. Electronic address: gykai@shnu.edu.cn.
authors.forename: Guoyin
authors.initials: G
authors.lastname: Kai
mesh_terms: D001483:Base Sequence; D001937:Brassica / Q000201:enzymology*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D015870:Gene Expression; D018517:Plant Roots / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics; D013482:Superoxide Dismutase / Q000235:genetics*; D015032:Zinc / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D013482:Superoxide Dismutase; D015032:Zinc
keywords: Abiotic stress; Cu/Zn SOD gene; Gene expression analysis; Non-heading Chinese cabbage (Brassica campestris ssp. chinensis)