title: Fluorescence characteristics and lifetime images of photosensitizers of talaporfin sodium and sodium pheophorbide a in normal and cancer cells.
abstract: Fluorescence spectra and fluorescence lifetime images of talaporfin sodium and sodium-pheophorbide a, which can be regarded as photosensitizers for photodynamic therapy, were measured in normal and cancer cells. The reduction of the fluorescence intensity by photoirradiation was observed for both photosensitizers in both cells, but the quenching rate was much faster in cancer cells than in normal cells. These results are explained in terms of the excessive generation of reactive oxygen species via photoexcitation of these photosensitizers in cancer cells. The fluorescence lifetimes of both photosensitizers in cancer cells are different from those in normal cells, which originates from the different intracellular environments around the photosensitizers between normal and cancer cells.
authors.affiliation: Research Institute for Electronic Science (RIES), Hokkaido University, Sapporo 001-0020, Japan. kaawasthi@gmail.com.
authors.forename: Kamlesh
authors.initials: K
authors.lastname: Awasthi
authors.affiliation: Graduate School of Photonic Science, Chitose Institute for Science and Technology, Chitose 066-8655, Japan. h13st@yahoo.co.jp.
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Yamamoto
authors.affiliation: Graduate School of Photonic Science, Chitose Institute for Science and Technology, Chitose 066-8655, Japan. b2102320@photon.chitose.ac.jp.
authors.forename: Kazunari
authors.initials: K
authors.lastname: Furuya
authors.affiliation: Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba-ku, Sendai 980-8578, Japan. takan@m.tohoku.ac.jp.
authors.forename: Takakazu
authors.initials: T
authors.lastname: Nakabayashi
authors.affiliation: Graduate School of Photonic Science, Chitose Institute for Science and Technology, Chitose 066-8655, Japan. liliming@photon.chitose.ac.jp.
authors.forename: Liming
authors.initials: L
authors.lastname: Li
authors.affiliation: Research Institute for Electronic Science (RIES), Hokkaido University, Sapporo 001-0020, Japan. nohta@nctu.edu.tw.
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Ohta
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002734:Chlorophyll / Q000031:analogs & derivatives* / Q000737:chemistry; D017319:Photosensitizing Agents / Q000737:chemistry*; D011166:Porphyrins / Q000737:chemistry*; D051381:Rats; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D017319:Photosensitizing Agents; D011166:Porphyrins; D002734:Chlorophyll; C032623:pheophorbide a; C053434:Talaporfin
keywords: cancer cells; fluorescence lifetime; fluorescence spectrum; photodynamic therapy; reactive oxygen species; sodium pheophorbide a; talaporfin sodium