title: Determination of the structure of the O-antigen and the lipid A from the entomopathogenic bacterium Pseudomonas entomophila lipopolysaccharide along with its immunological properties.
abstract: The structure and the immunology of the lipopolysaccharide (LPS) of Pseudomonas entomophila, an entomopathogenic bacterium isolated from the fruit fly Drosophila melanogaster, was characterized. The O-antigen portion was established and resulted to be built up of a repetitive unit constituted by four monosaccharide residues, all L configured, all deoxy at C-6 and with an acetamido function at C-2: →3)-α-l-FucNAc-(1→4)-α-l-FucNAc-(1→3)-α-l-FucNAc-(1→3)-β-l-QuiNAc-(1→ The structural analysis of lipid A, showed a mixture of different species. The diphosphorylated glucosamine backbone carries six fatty acids consistent with the composition C10:0 3(OH), C12:0 2(OH) and C12:0 3(OH), whereas other species differs by the number of phosphates and/or of fatty acids. The immunology experiments demonstrated that the LPS structure of P. entomophila displayed a low ability to engage the TLR4-mediated signaling correlated to a significant antagonistic activity toward hexa-acylated LPS structures.
authors.affiliation: Dipartimento di Scienze Chimiche, Università di Napoli "Federico II", Complesso Universitario Monte S.Angelo, Via Cintia 4, 80126 Napoli, Italy.
authors.forename: Immacolata
authors.initials: I
authors.lastname: Speciale
authors.affiliation: Dipartimento di Biologia e Biotecnologie "C. Darwin" Sapienza-Università di Roma, Piazzale Aldo Moro, 5, 00185 Roma, Italy; Istituto Pasteur-Fondazione Cenci Bolognetti, Piazzale Aldo Moro 5, 00185 Roma-Italia, Sapienza-Università di Roma, 00185 Roma, Italy.
authors.forename: Ida
authors.initials: I
authors.lastname: Paciello
authors.affiliation: Dipartimento di Biologia e Biotecnologie "C. Darwin" Sapienza-Università di Roma, Piazzale Aldo Moro, 5, 00185 Roma, Italy.
authors.forename: Luigi Lembo
authors.initials: LL
authors.lastname: Fazio
authors.affiliation: Istituto di Chimica e Tecnologia dei Polimeri-ICTP-CNR, Via P. Gaifami 18, 95126 Catania, Italy.
authors.forename: Luisa
authors.initials: L
authors.lastname: Sturiale
authors.affiliation: Istituto di Chimica e Tecnologia dei Polimeri-ICTP-CNR, Via P. Gaifami 18, 95126 Catania, Italy.
authors.forename: Angelo
authors.initials: A
authors.lastname: Palmigiano
authors.affiliation: Dipartimento di Scienze Chimiche, Università di Napoli "Federico II", Complesso Universitario Monte S.Angelo, Via Cintia 4, 80126 Napoli, Italy.
authors.forename: Rosa
authors.initials: R
authors.lastname: Lanzetta
authors.affiliation: Dipartimento di Scienze Chimiche, Università di Napoli "Federico II", Complesso Universitario Monte S.Angelo, Via Cintia 4, 80126 Napoli, Italy.
authors.forename: Michelangelo
authors.initials: M
authors.lastname: Parrilli
authors.affiliation: Istituto di Chimica e Tecnologia dei Polimeri-ICTP-CNR, Via P. Gaifami 18, 95126 Catania, Italy.
authors.forename: Domenico
authors.initials: D
authors.lastname: Garozzo
authors.affiliation: Global Health Institute, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
authors.forename: Bruno
authors.initials: B
authors.lastname: Lemaitre
authors.affiliation: Dipartimento di Biologia e Biotecnologie "C. Darwin" Sapienza-Università di Roma, Piazzale Aldo Moro, 5, 00185 Roma, Italy; Istituto Pasteur-Fondazione Cenci Bolognetti, Piazzale Aldo Moro 5, 00185 Roma-Italia, Sapienza-Università di Roma, 00185 Roma, Italy.
authors.forename: Maria Lina
authors.initials: ML
authors.lastname: Bernardini
authors.affiliation: Dipartimento di Scienze Chimiche, Università di Napoli "Federico II", Complesso Universitario Monte S.Angelo, Via Cintia 4, 80126 Napoli, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Molinaro
authors.affiliation: Dipartimento di Agraria, Via Università 100, 80055 Portici, NA, Italy. Electronic address: decastro@unina.it.
authors.forename: Cristina
authors.initials: C
authors.lastname: De Castro
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster / Q000276:immunology / Q000382:microbiology; D004926:Escherichia coli; D005227:Fatty Acids / Q000737:chemistry; D006801:Humans; D008050:Lipid A / Q000737:chemistry* / Q000276:immunology; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000276:immunology; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D019081:O Antigens / Q000737:chemistry* / Q000276:immunology; D011549:Pseudomonas / Q000737:chemistry* / Q000276:immunology / Q000472:pathogenicity; D051197:Toll-Like Receptor 4 / Q000276:immunology / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D008050:Lipid A; D008070:Lipopolysaccharides; D019081:O Antigens; C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4
keywords: Drosophila melanogaster; Innate immunity; Lipopolysaccharide; Pseudomonas entomophila; Structural analysis