title: Osthole promotes anti-tumor immune responses in tumor-bearing mice with hepatocellular carcinoma.
abstract: Osthole, a natural coumarin derivative, has been shown to have anti-tumor and anti-inflammatory activity. However, the effect of osthole on anti-tumor immune responses in tumor-bearing mice has not yet been reported. In the present study, osthole treatment did not affect the weight and the coefficient of thymus and spleen in tumor-bearing mice with hepatocellular carcinoma (HCC). However, osthole administration significantly elevated the proportion and number of the splenic CD8(+) T cells, the proportion of CD4(+) T and CD8(+) T cells in tumor tissues, and the levels of IL-2 and TNF-α in the serum of HCC tumor-bearing mice. Our results suggested that osthole could promote the activation of the tumor-infiltrating CD4(+) T and CD8(+) T cells, and elevate the proportion of CD4(+) and CD8(+) effector T cells. Osthole treatment also significantly decreased the proportion of CD4(+)CD25(+)Foxp3(+) regulatory T cells in the spleen. Taken together, osthole could enhance the T cell mediated anti-tumor immune responses in the tumor-bearing mice with HCC.
authors.affiliation: Laboratory of Cellular and Molecular Tumor Immunology, Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and Medical Sciences, Soochow University , Suzhou , P.R. China .
authors.forename: Lurong
authors.initials: L
authors.lastname: Zhang
authors.forename: Guorong
authors.initials: G
authors.lastname: Jiang
authors.forename: Fei
authors.initials: F
authors.lastname: Yao
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Liang
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.forename: Heng
authors.initials: H
authors.lastname: Xu
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
authors.forename: Xiao
authors.initials: X
authors.lastname: Yu
authors.forename: Haiyan
authors.initials: H
authors.lastname: Liu
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000473:pathology; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D045744:Cell Line, Tumor; D003374:Coumarins / Q000494:pharmacology*; D007111:Immunity, Cellular / Q000187:drug effects*; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D051379:Mice; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000473:pathology
chemical_list: D000276:Adjuvants, Immunologic; D003374:Coumarins; C046627:osthol
keywords: Immune response; Osthole; T cell; liver; tumor