title: Free radical scavenging, antidiarrheal and anthelmintic activity of Pistia stratiotes L. extracts and its phytochemical analysis.
abstract: In this phyto-pharmacological screening of Pistia stratiotes L leaf and root extracts each separately in two different solvents demonstrated its potential medicinal value. Apparent antioxidant value is demonstrated by DPPH, Nitric oxide scavenging and Ferric ion reducing method. Additionally, total flavonoid and phenolic compounds were measured. The leaf methanolic extract scavenged both nitric oxide (NO) and DPPH radical with a dose dependent manner. But the pet ether fraction of root was found to have highest efficacy in Fe(3±) reducing power assay. Flavonoid was found to contain highest in the pet ether fraction of root (411.35mg/g) in terms of quercetin equivalent, similarly highest amount (34.96mg/g) of total phenolic compounds (assayed as gallic acid equivalents) were found to contain in the same fraction. The methanolic fractions appeared less cytotoxic compared to pet ether extracts. The plant extracts caused a dose dependent decrease in faecal droppings in both castor oil and magnesium sulphate induced diarrhea, where as leaf extracts in each solvent appeared most effective. Also, the plant extracts showed anthelmintic activity in earthworm by inducing paralysis and death in a dose dependent manner. At highest doses (50 mg/ml) all fractions were almost effective as the positive control piperazine citrate (10 mg/ml). Thus, besides this cytotoxic effect it's traditional claim for therapeutic use can never be overlooked.
authors.affiliation: Department of Pharmacy, East West University, Aftabnagar, Dhaka, Bangladesh.
authors.forename: Mohammed Faisal
authors.initials: MF
authors.lastname: Bin Karim
authors.affiliation: Department of Pharmacy, Primeasia University, 9 Banani C/A, Dhaka, Bangladesh.
authors.forename: Hasan
authors.initials: H
authors.lastname: Imam
authors.affiliation: Diabetes, Obesity and Genomics Research Group, Clinical Investigation Centre, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Md Moklesur-Rahman
authors.initials: MM
authors.lastname: Sarker
authors.affiliation: Department of Pharmacy, Jahangirnagar University, Savar, Dhaka, Bangladesh.
authors.forename: Nizam
authors.initials: N
authors.lastname: Uddin
authors.affiliation: Department of Pharmacy, Jahangirnagar University, Savar, Dhaka, Bangladesh.
authors.forename: Nahid
authors.initials: N
authors.lastname: Hasan
authors.affiliation: Department of Pharmacy, Jahangirnagar University, Savar, Dhaka, Bangladesh.
authors.forename: Nirmala
authors.initials: N
authors.lastname: Paul
authors.affiliation: Department of Pharmacy, Primeasia University, 9 Banani C/A, Dhaka, Bangladesh.
authors.forename: Tahmina
authors.initials: T
authors.lastname: Haque
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D000930:Antidiarrheals / Q000494:pharmacology*; D029064:Araceae; D001156:Artemia / Q000187:drug effects; D001713:Biphenyl Compounds / Q000378:metabolism; D003672:Defecation / Q000187:drug effects*; D005419:Flavonoids / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000494:pharmacology*; D007202:Indicators and Reagents / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D051379:Mice; D009569:Nitric Oxide; D009835:Oligochaeta / Q000187:drug effects*; D010084:Oxidation-Reduction; D010636:Phenols / Q000494:pharmacology; D064209:Phytochemicals / Q000494:pharmacology; D010851:Picrates / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves; D018517:Plant Roots
chemical_list: D000871:Anthelmintics; D000930:Antidiarrheals; D001713:Biphenyl Compounds; D005419:Flavonoids; D016166:Free Radical Scavengers; D007202:Indicators and Reagents; D010636:Phenols; D064209:Phytochemicals; D010851:Picrates; D010936:Plant Extracts; D009569:Nitric Oxide; C004931:1,1-diphenyl-2-picrylhydrazyl; D007501:Iron