title: Identification of New Natural CphA Metallo-β-Lactamases CphA4 and CphA5 in Aeromonas veronii and Aeromonas hydrophila Isolates from Municipal Sewage in Central Italy.
abstract: Two new natural CphA metallo-β-lactamases, the CphA4 and CphA5 enzymes, were identified in water samples from municipal sewage in central Italy. Compared to CphA, the CphA4 and CphA5 enzymes showed numerous point mutations. These enzymes have a narrow spectrum of substrates focused on carbapenems only. CphA5 showed kcat values about 40-, 12-, and 97-fold higher than those observed for CphA4 versus imipenem, ertapenem, and biapenem, respectively.
authors.affiliation: Dipartimento di Scienze Cliniche Applicate e Biotecnologiche, Università degli Studi dell'Aquila, L'Aquila, Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Bottoni
authors.affiliation: Dipartimento di Scienze Cliniche Applicate e Biotecnologiche, Università degli Studi dell'Aquila, L'Aquila, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Marcoccia
authors.affiliation: Dipartimento di Scienze Cliniche Applicate e Biotecnologiche, Università degli Studi dell'Aquila, L'Aquila, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Compagnoni
authors.affiliation: Dipartimento di Scienze Cliniche Applicate e Biotecnologiche, Università degli Studi dell'Aquila, L'Aquila, Italy.
authors.forename: Martina
authors.initials: M
authors.lastname: Colapietro
authors.affiliation: Dipartimento di Scienze Cliniche Applicate e Biotecnologiche, Università degli Studi dell'Aquila, L'Aquila, Italy.
authors.forename: Alessia
authors.initials: A
authors.lastname: Sabatini
authors.affiliation: Dipartimento di Scienze Cliniche Applicate e Biotecnologiche, Università degli Studi dell'Aquila, L'Aquila, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Celenza
authors.affiliation: Dipartimento di Scienze Cliniche Applicate e Biotecnologiche, Università degli Studi dell'Aquila, L'Aquila, Italy.
authors.forename: Bernardetta
authors.initials: B
authors.lastname: Segatore
authors.affiliation: Dipartimento di Scienze Cliniche Applicate e Biotecnologiche, Università degli Studi dell'Aquila, L'Aquila, Italy.
authors.forename: Maria Giovanna
authors.initials: MG
authors.lastname: Maturo
authors.affiliation: Dipartimento di Scienze Cliniche Applicate e Biotecnologiche, Università degli Studi dell'Aquila, L'Aquila, Italy.
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Amicosante
authors.affiliation: Dipartimento di Scienze Cliniche Applicate e Biotecnologiche, Università degli Studi dell'Aquila, L'Aquila, Italy perilli@univaq.it.
authors.forename: Mariagrazia
authors.initials: M
authors.lastname: Perilli
mesh_terms: D016980:Aeromonas hydrophila / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D015780:Carbapenems / Q000494:pharmacology; D000077727:Ertapenem; D015378:Imipenem / Q000494:pharmacology; D007558:Italy; D008969:Molecular Sequence Data; D017354:Point Mutation / Q000235:genetics; D012722:Sewage / Q000382:microbiology*; D013845:Thienamycins / Q000494:pharmacology; D001618:beta-Lactamases / Q000235:genetics*; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D012722:Sewage; D013845:Thienamycins; D047090:beta-Lactams; D015378:Imipenem; D001618:beta-Lactamases; C082063:cphA protein, Aeromonas hydrophila; D000077727:Ertapenem; C065257:biapenem