title: Quantitative investigation of the poly-adenine DNA dissociation from the surface of gold nanoparticles.
abstract: In recent years, poly adenine (polyA) DNA functionalized gold nanoparticles (AuNPs) free of modifications was fabricated with high density of DNA attachment and high hybridization ability similar to those of its thiolated counterpart. This nanoconjugate utilized poly adenine as an anchoring block for binding with the AuNPs surface thereby facilitated the appended recognition block a better upright conformation for hybridization, demonstrating its great potential to be a tunable plasmonic biosensor. It's one of the key points for any of the practical applications to maintaining stable conjugation between DNA oligonucleotides and gold nanoparticles under various experimental treatments. Thus, in this research, we designed a simple but sensitive fluorescence turn-on strategy to systematically investigate and quantified the dissociation of polyA DNA on gold nanoparticles in diverse experimental conditions. DNA desorbed spontaneously as a function of elevated temperature, ion strength, buffer pH, organic solvents and keeping time. What's more, evaluating this conjugate stability as affected by the length of its polyA anchor was another crucial aspect in our study. With the improved understanding from these results, we were able to control some of our experimental conditions to maintain a good stability of this kind of polyA DNA-AuNPs nanoconjugates.
authors.affiliation: Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan 610064, China.
authors.forename: Weiwen
authors.initials: W
authors.lastname: Lu
authors.affiliation: Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Wang
authors.affiliation: Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan 610064, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Johns Hopkins University, 3400 North Charles Street, Baltimore, Maryland 21218, US.
authors.forename: Ziang
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: UCB Pharma, Slough, UK.
authors.forename: Jiye
authors.initials: J
authors.lastname: Shi
authors.affiliation: Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Zuo
authors.affiliation: Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
authors.forename: Dun
authors.initials: D
authors.lastname: Pan
mesh_terms: D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D058726:Nanoconjugates / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000737:chemistry; D012997:Solvents / Q000737:chemistry
chemical_list: D058726:Nanoconjugates; D012997:Solvents; D011061:Poly A; D006046:Gold; D004247:DNA