title: RNA interference: Applications and advances in insect toxicology and insect pest management.
abstract: Since its discovery, RNA interference (RNAi) has revolutionized functional genomic studies due to its sequence-specific nature of post-transcriptional gene silencing. In this paper, we provide a comprehensive review of the recent literature and summarize the current knowledge and advances in the applications of RNAi technologies in the field of insect toxicology and insect pest management. Many recent studies have focused on identification and validation of the genes encoding insecticide target proteins, such as acetylcholinesterases, ion channels, Bacillus thuringiensis receptors, and other receptors in the nervous system. RNAi technologies have also been widely applied to reveal the role of genes encoding cytochrome P450 monooxygenases, carboxylesterases, and glutathione S-transferases in insecticide detoxification and resistance. More recently, studies have focused on understanding the mechanism of insecticide-mediated up-regulation of detoxification genes in insects. As RNAi has already shown great potentials for insect pest management, many recent studies have also focused on host-induced gene silencing, in which several RNAi-based transgenic plants have been developed and tested as proof of concept for insect pest management. These studies indicate that RNAi is a valuable tool to address various fundamental questions in insect toxicology and may soon become an effective strategy for insect pest management.
authors.affiliation: Department of Entomology, Kansas State University, Manhattan, KS 66506-4004, USA.
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
authors.affiliation: Department of Entomology, Kansas State University, Manhattan, KS 66506-4004, USA.
authors.forename: Moustapha
authors.initials: M
authors.lastname: Soumaila Issa
authors.affiliation: Department of Entomology, Kansas State University, Manhattan, KS 66506-4004, USA.
authors.forename: Anastasia M W
authors.initials: AM
authors.lastname: Cooper
authors.affiliation: Department of Entomology, Kansas State University, Manhattan, KS 66506-4004, USA. Electronic address: kzhu@ksu.edu.
authors.forename: Kun Yan
authors.initials: KY
authors.lastname: Zhu
mesh_terms: D000818:Animals; D019476:Insect Proteins / Q000235:genetics*; D007313:Insecta; D010572:Pest Control, Biological; D034622:RNA Interference
chemical_list: D019476:Insect Proteins
keywords: Detoxification; Insect toxicology; Insecticide resistance; Pest management; RNA interference