title: Hokkaido genotype of Puumala virus in the grey red-backed vole (Myodes rufocanus) and northern red-backed vole (Myodes rutilus) in Siberia.
abstract: Three species of Myodes voles known to harbor hantaviruses include the bank vole (Myodes glareolus), which serves as the reservoir host of Puumala virus (PUUV), the prototype arvicolid rodent-borne hantavirus causing hemorrhagic fever with renal syndrome (HFRS) in Europe, and the grey red-backed vole (Myodes rufocanus) and royal vole (Myodes regulus) which carry two PUUV-like hantaviruses, designated Hokkaido virus (HOKV) and Muju virus (MUJV), respectively. To ascertain the hantavirus harbored by the northern red-backed vole (Myodes rutilus), we initially screened sera from 233 M. rutilus, as well as from 90 M. rufocanus and 110 M. glareolus, captured in western and eastern Siberia during June 2007 to October 2009, for anti-hantaviral antibodies. Thereafter, lung tissues from 44 seropositive voles were analyzed for hantavirus RNA by reverse transcription-polymerase chain reaction. Partial L-, M- and S-segment sequences, detected in M. rutilus and M. rufocanus, were closely related to HOKV, differing from previously published L-, M- and S-segment sequences of HOKV by 17.8-20.2%, 15.9-23.4% and 15.0-17.0% at the nucleotide level and 2.6-7.9%, 1.3-6.3% and 1.2-4.0% at the amino acid level, respectively. Alignment and comparison of hantavirus sequences from M. glareolus trapped in Tyumen Oblast showed very high sequence similarity to the Omsk lineage of PUUV. Phylogenetic analysis, using neighbor-joining, maximal likelihood and Bayesian methods, showed that HOKV strains shared a common ancestry with PUUV and exhibited geographic-specific clustering. This report provides the first molecular evidence that both M. rutilus and M. rufocanus harbor HOKV, which might represent a genetic variant of PUUV.
authors.affiliation: State Research Center of Virology and Biotechnology "Vector", Koltsovo, Russia. Electronic address: yashina@vector.nsc.ru.
authors.forename: Liudmila N
authors.initials: LN
authors.lastname: Yashina
authors.affiliation: Institute of Systematics and Ecology of Animals, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Abramov
authors.affiliation: Institute of Systematics and Ecology of Animals, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
authors.forename: Tamara A
authors.initials: TA
authors.lastname: Dupal
authors.affiliation: Federal Budgetary Scientific Center for Family Health and Human Reproduction Problems, Irkutsk, Russia.
authors.forename: Galina A
authors.initials: GA
authors.lastname: Danchinova
authors.affiliation: State Research Center of Virology and Biotechnology "Vector", Koltsovo, Russia.
authors.forename: Boris S
authors.initials: BS
authors.lastname: Malyshev
authors.affiliation: School of Medicine and Biomedical Sciences, State University of New York at Buffalo, New York, NY, USA.
authors.forename: John
authors.initials: J
authors.lastname: Hay
authors.affiliation: John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI, USA.
authors.forename: Se Hun
authors.initials: SH
authors.lastname: Gu
authors.affiliation: John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI, USA. Electronic address: ryanagih@hawaii.edu.
authors.forename: Richard
authors.initials: R
authors.lastname: Yanagihara
mesh_terms: D000818:Animals; D003411:Arvicolinae / Q000821:virology*; D004197:Disease Reservoirs / Q000821:virology; D005838:Genotype; D005843:Geography; D006480:Hemorrhagic Fever with Renal Syndrome / Q000453:epidemiology / Q000635:transmission / Q000821:virology; D010802:Phylogeny; D015995:Prevalence; D029262:Puumala virus / Q000145:classification / Q000235:genetics*; D012367:RNA, Viral; D012800:Siberia / Q000453:epidemiology
chemical_list: D012367:RNA, Viral
keywords: Hantavirus; Myodes voles; Puumala virus; Siberia