title: Genome-wide functional genomic and transcriptomic analyses for genes regulating sensitivity to vorinostat.
abstract: Identification of mechanisms of resistance to histone deacetylase inhibitors, such as vorinostat, is important in order to utilise these anticancer compounds more efficiently in the clinic. Here, we present a dataset containing multiple tiers of stringent siRNA screening for genes that when knocked down conferred sensitivity to vorinostat-induced cell death. We also present data from a miRNA overexpression screen for miRNAs contributing to vorinostat sensitivity. Furthermore, we provide transcriptomic analysis using massively parallel sequencing upon knockdown of 14 validated vorinostat-resistance genes. These datasets are suitable for analysis of genes and miRNAs involved in cell death in the presence and absence of vorinostat as well as computational biology approaches to identify gene regulatory networks.
authors.affiliation: Cancer Therapeutic Program, The Peter MacCallum Cancer Centre , St Andrews Place, East Melbourne, Victoria 3002, Australia.
authors.forename: Katrina J
authors.initials: KJ
authors.lastname: Falkenberg
authors.affiliation: Victorian Centre for Functional Genomics, The Peter MacCallum Cancer Centre , St Andrews Place, East Melbourne, Victoria 3002, Australia.
authors.forename: Cathryn M
authors.initials: CM
authors.lastname: Gould
authors.affiliation: Cancer Therapeutic Program, The Peter MacCallum Cancer Centre , St Andrews Place, East Melbourne, Victoria 3002, Australia ; Sir Peter MacCallum Department of Oncology, The University of Melbourne , Parkville, Victoria 3052, Australia.
authors.forename: Ricky W
authors.initials: RW
authors.lastname: Johnstone
authors.affiliation: Victorian Centre for Functional Genomics, The Peter MacCallum Cancer Centre , St Andrews Place, East Melbourne, Victoria 3002, Australia ; Sir Peter MacCallum Department of Oncology, The University of Melbourne , Parkville, Victoria 3052, Australia.
authors.forename: Kaylene J
authors.initials: KJ
authors.lastname: Simpson
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects* / Q000235:genetics*; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D023281:Genomics; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology*; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology*; D034741:RNA, Small Interfering; D000077337:Vorinostat
chemical_list: D000970:Antineoplastic Agents; D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; D034741:RNA, Small Interfering; D000077337:Vorinostat