title: Generation of aroE overexpression mutant of Bacillus megaterium for the production of shikimic acid.
abstract: BACKGROUND
  Shikimic acid, the sole chemical building block for the antiviral drug oseltamivir (Tamiflu®), is one of the potent pharmaceutical intermediates with three chiral centers. Here we report a metabolically engineered recombinant Bacillus megaterium strain with aroE (shikimate dehydrogenase) overexpression for the production of shikimic acid.
  
  
  RESULTS
  In a 7 L bioreactor, 4.2 g/L shikimic acid was obtained using the recombinant strain over 0.53 g/L with the wild type. The enhancement of total shikimate dehydrogenase activity was 2.13-fold higher than the wild type. Maximum yield of shikimic acid (12.54 g/L) was obtained with fructose as carbon source. It was isolated from the fermentation broth using amberlite IRA-400 resin and 89% purity of the product was achieved.
  
  
  CONCLUSIONS
  This will add up a new organism in the armory for the fermentation based production which is better over plant based extraction and chemical synthesis of shikimic acid.
authors.affiliation: Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research, Sector 67, S. A. S., Nagar, 160062, Punjab, India. saptasandhan007@gmail.com.
authors.forename: Saptarshi
authors.initials: S
authors.lastname: Ghosh
authors.affiliation: Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research, Sector 67, S. A. S., Nagar, 160062, Punjab, India. ucbanerjee@niper.ac.in.
authors.forename: Uttam Chand
authors.initials: UC
authors.lastname: Banerjee
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry*; D001410:Bacillus megaterium; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D005285:Fermentation; D008958:Models, Molecular; D062105:Molecular Docking Simulation; D012765:Shikimic Acid / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D012765:Shikimic Acid; D000429:Alcohol Oxidoreductases; C022641:Shikimate dehydrogenase