title: Spectroscopic Evaluation of DNA-Borate Interactions.
abstract: We describe the binding characteristics of two natural borates (colemanite and ulexite) to calf thymus DNA by UV-vis absorbance spectroscopy, circular dichroism (CD) spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, and a competitive DNA binding assay. Our results suggest that colemanite and ulexite interact with calf thymus DNA under a non-intercalative mode of binding and do not alter the secondary structure of the DNA helix. The FT-IR spectroscopy results indicate that the two borates might interact with DNA through sugar-phosphate backbone binding.
authors.affiliation: Institute of Materials Science & Nanotechnology, Bilkent University, 06800, Bilkent, Ankara, Turkey.|UNAM-National Nanotechnology Research Center, Bilkent University, Ankara, 06800, Turkey.
authors.forename: Ayse
authors.initials: A
authors.lastname: Ozdemir
authors.affiliation: Institute of Materials Science & Nanotechnology, Bilkent University, 06800, Bilkent, Ankara, Turkey.|UNAM-National Nanotechnology Research Center, Bilkent University, Ankara, 06800, Turkey.
authors.forename: Omer Faruk
authors.initials: OF
authors.lastname: Sarioglu
authors.affiliation: Department of Medical Biology and Genetics, Faculty of Medicine, Gazi University, 06500, Besevler, Ankara, Turkey. ttekinay@gazi.edu.tr.|Life Sciences Application and Research Center, Gazi University, 06830, Golbasi, Ankara, Turkey. ttekinay@gazi.edu.tr.
authors.forename: Turgay
authors.initials: T
authors.lastname: Tekinay
mesh_terms: D000818:Animals; D001881:Borates / Q000737:chemistry*; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D004996:Ethidium / Q000737:chemistry; D009690:Nucleic Acid Conformation; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013950:Thymus Gland
chemical_list: D001881:Borates; D004247:DNA; C427203:calf thymus DNA; D004996:Ethidium
keywords: Boron; Calf thymus DNA; DNA binding; Deoxyribose sugar; Spectroscopy