title: Study of NGEP expression in androgen sensitive prostate cancer cells: A potential target for immunotherapy.
abstract: BACKGROUND
  Prostate cancer is one of the leading causes of cancer deaths among men. New gene expressed in prostate (NGEP), is a prostate-specific gene expressed only in normal prostate and prostate cancer tissue. Because of its selective expression in prostate cancer cell surface, NGEP is a potential immunotherapeutic target. To target the NGEP in prostate cancer, it is essential to investigate its expression in prostate cancer cells.
  
  
  METHODS
  In the present study, we investigated NGEP expression in LNCaP and DU145 cells by real time and RT-PCR, flow cytometric and immunocytochemical analyses.
  
  
  RESULTS
  Real time and RT-PCR analyses of NGEP expression showed that NGEP was expressed in the LNCaP cells but not in DU145 cells. The detection of NGEP protein by flow cytometric and immunocytochemistry analyses indicated that NGEP protein was weakly expressed only in LNCaP cell membrane.
  
  
  CONCLUSIONS
  Our results demonstrate that LNCaP cell line is more suitable than DU145 for NGEP expression studies; however, its low-level expression is a limiting issue. NGEP expression may be increased by androgen supplementation of LNCaP cell culture medium.
authors.affiliation: PhD student, Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran & Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran. monirehmohsenzadegan@gmail.com.
authors.forename: Monireh
authors.initials: M
authors.lastname: Mohsenzadegan
authors.affiliation: Associate Professor, Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran & Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran. nadertajik@yahoo.com.
authors.forename: Nader
authors.initials: N
authors.lastname: Tajik
authors.affiliation: Associate Professor, Oncopathology Research Centre and Department of Pathology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. zahra.madjd@yahoo.com.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Madjd
authors.affiliation: Associate Professor, Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. m_shekarabi@yahoo.com.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Shekarabi
authors.affiliation: Professor, Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran, Department of Medical Biotechnology, School of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran & Food and Drug Laboratory, Fardis, Alborz, Iran. mahdiy@yahoo.com.
authors.forename: Mohammad M
authors.initials: MM
authors.lastname: Farajollahi
keywords: DU145 cell line; LNCaP cell line; NGEP; Prostate cancer