title: Various fractions of Hypericum x moserianum and Hypericum ericoides possess antiglycation, anti-lipid peroxidation, antioxidative activities and non-toxic effects in vitro.
abstract: In the present study, two species Hypericum x moserianum and Hypericum ericoides which belong to genus Hypericum were evaluated for their potential antiglycation, antioxidant, anti lipid peroxidation and cytotoxic activities. These species are widely used in folk medicine and to the best of our knowledge there were no previous reports regarding antioxidant, anti-glycation and cytotoxicity studies of these species. Among the crude methanol extracts and fractions of both the species, the ethyl acetate fraction of H. x moserianum exhibited promising antioxidant activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) with IC50 129.084±1.215μg/ml, followed by methanol extract (IC50=232.083 ± 1.215μg/ml) and aqueous fraction (IC50=266.962 ±2.213 μg/ml). The ethyl acetate fraction of H. ericoides exhibited IC50 value of 295.088 ± 2.320 μg/ml. In antiglycation assay, the ethyl acetate fraction of H. x moserianum showed 52.096% inhibition at 500μg/ml. For lipid peroxidation assay, the dichloromethane, aqueous and n-hexane fractions of H. x moserianum showed 67.241, 66.147 and 64.213% inhibition respectively, while aqueous fraction of H. ericoides exhibited 67.404% inhibition at 500μg/ml. In cytotoxicity assay, all fractions of both the species were found to be non-toxic on mouse fibroblast 3T3 cells with IC50 value greater than 30μg/ml as compared to cycloheximide with IC50 value 0.073±0.1μg/ml used as a standard. It was concluded from the study that among the two species, crude methanolic and ethyl acetate fractions were more active regarding the antioxidant, anti-glycation activities while dichloromethane, aqueous and n-hexane fractions possessed anti-lipid peroxidation activity.
authors.affiliation: Department of Biochemistry, University of Health Sciences, Lahore, Pakistan / Department of Biological Sciences and Chemistry, University of Nizwa, Nizwa-616, Oman.
authors.forename: Ghulam
authors.initials: G
authors.lastname: Abbas
authors.affiliation: Department of Pharmacology, University of Health Sciences, Lahore, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Shahzad
authors.affiliation: Department of Botany, Lahore College for Women University, Lahore, Pakistan.
authors.forename: Zeb
authors.initials: Z
authors.lastname: Saddiqe
authors.affiliation: ASAB, National University of Sciences and Technology (NUST), Islamabad, Pakistan.
authors.forename: M Jawad
authors.initials: MJ
authors.lastname: Hassan
authors.affiliation: International Center for Chemical and Biological Sciences, HEJ RIC, University of Karachi, Karachi, Pakistan.
authors.forename: Sumbal
authors.initials: S
authors.lastname: Saba
authors.affiliation: International Center for Chemical and Biological Sciences, HEJ RIC, University of Karachi, Karachi, Pakistan.
authors.forename: Jamal
authors.initials: J
authors.lastname: Rafique
authors.affiliation: International Center for Chemical and Biological Sciences, HEJ RIC, University of Karachi, Karachi, Pakistan.
authors.forename: Rizwana
authors.initials: R
authors.lastname: Malik
authors.affiliation: UoN Chair of Oman's Medicinal Plants and Marine Natural Products, University of Nizwa, Nizwa-616, Oman.
authors.forename: Hidayat
authors.initials: H
authors.lastname: Hussain
mesh_terms: D016475:3T3 Cells / Q000187:drug effects; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000378:metabolism; D006031:Glycosylation / Q000187:drug effects*; D020902:Hypericum; D066298:In Vitro Techniques; D007202:Indicators and Reagents / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D051379:Mice; D010851:Picrates / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D007202:Indicators and Reagents; D010851:Picrates; D010936:Plant Extracts; C004931:1,1-diphenyl-2-picrylhydrazyl