title: N-Stearoylethanolamine suppresses the pro-inflammatory cytokines production by inhibition of NF-κB translocation.
abstract: N-Stearoylethanolamine (NSE) is a minor lipid that belongs to the N-Acylethanolamines family that mediates a wide range of biological processes. This study investigates the mechanisms of anti-inflammatory action of NSE on different model systems. Namely, we estimated the effect of NSE on inflammatory cytokines mRNA level (leukemia cells L1210), cytokines content (serum and LPS-stimulated macrophages) and nuclear translocation of NF-κB (peritoneal macrophages LPS-stimulated and isolated from rats with obesity-induced insulin resistance). The results indicated that NSE dose-dependently inhibits the IL-1 and IL-6 mRNA level in L1210 cells. Furthermore, the NSE treatment triggered a normalization of serum TNF-α level in insulin resistant rats and a reduction of medium IL-1 level in LPS-activated peritoneal macrophages. These NSE's effects were associated with the inhibition of nuclear NF-κB translocation in rat peritoneal macrophages.
authors.affiliation: Department of Lipid Biochemistry,(3) O.V. Palladin Institute of Biochemistry of National Academy of Science of Ukraine, Leontovich St. 9, 01601, Kyiv, Ukraine. Electronic address: kievlipids@gmail.com.
authors.forename: Andrey G
authors.initials: AG
authors.lastname: Berdyshev
authors.affiliation: Department of Lipid Biochemistry,(3) O.V. Palladin Institute of Biochemistry of National Academy of Science of Ukraine, Leontovich St. 9, 01601, Kyiv, Ukraine. Electronic address: kosiakova@hotmail.com.
authors.forename: Halyna V
authors.initials: HV
authors.lastname: Kosiakova
authors.affiliation: Department of Lipid Biochemistry,(3) O.V. Palladin Institute of Biochemistry of National Academy of Science of Ukraine, Leontovich St. 9, 01601, Kyiv, Ukraine. Electronic address: onop.89.av@mail.ru.
authors.forename: Oleksandra V
authors.initials: OV
authors.lastname: Onopchenko
authors.affiliation: Department of Regulation of Cell Proliferation and Apoptosis, Institute of Cell Biology of National Academy of Science of Ukraine, (4) Drahomanov St. 14/16, 79005, Lviv, Ukraine. Electronic address: rpanchuk@ukr.net.
authors.forename: Rostislav R
authors.initials: RR
authors.lastname: Panchuk
authors.affiliation: Department of Regulation of Cell Proliferation and Apoptosis, Institute of Cell Biology of National Academy of Science of Ukraine, (4) Drahomanov St. 14/16, 79005, Lviv, Ukraine. Electronic address: stoika.rostyslav@gmail.com.
authors.forename: Rostislav S
authors.initials: RS
authors.lastname: Stoika
authors.affiliation: Department of Lipid Biochemistry,(3) O.V. Palladin Institute of Biochemistry of National Academy of Science of Ukraine, Leontovich St. 9, 01601, Kyiv, Ukraine. Electronic address: ngula@biochem.kiev.ua.
authors.forename: Nadiya M
authors.initials: NM
authors.lastname: Hula
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000187:drug effects; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D016207:Cytokines / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D004983:Ethanolamines / Q000494:pharmacology*; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D008070:Lipopolysaccharides / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism*; D009765:Obesity / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D013229:Stearic Acids / Q000494:pharmacology*
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D004983:Ethanolamines; D007328:Insulin; D008070:Lipopolysaccharides; C408368:N-stearoylethanolamine; D016328:NF-kappa B; D012333:RNA, Messenger; D013229:Stearic Acids
keywords: Cytokines; Inflammation; Insulin resistance; N-Acylethanolamines; N-Stearoylethanolamine; NF-kB