title: Quantitative metabolic profiling of NMR spectral signatures of branched chain amino acids in blood serum.
abstract: Branched Chain Amino Acids (BCAAs) are related to different aspects of diseases like pathogenesis, diagnosis and even prognosis. While in some diseases, levels of all the BCAAs are perturbed; in some cases, perturbation occurs in one or two while the rest remain unaltered. In case of ischemic heart disease, there is an enhanced level of plasma leucine and isoleucine but valine level remains unaltered. In 'Hypervalinemia', valine is elevated in serum and urine, but not leucine and isoleucine. Therefore, identification of these metabolites and profiling of individual BCAA in a quantitative manner in body-fluid like blood plasma/serum have long been in demand. (1)H NMR resonances of the BCAAs overlap with each other which complicates quantification of individual BCAAs. Further, the situation is limited by the overlap of broad resonances of lipoprotein with the resonances of BCAAs. The widely used commercially available kits cannot differentially estimate the BCAAs. Here, we have achieved proper identification and characterization of these BCAAs in serum in a quantitative manner employing a Nuclear Magnetic Resonance-based technique namely T2-edited Correlation Spectroscopy (COSY). This approach can easily be extended to other body fluids like bile, follicular fluids, saliva, etc.
authors.affiliation: Department of Chemical Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai, 400005, India.|Laboratory of Clinical Investigation, National Institute on Aging, 251 Bayview Boulevard, Baltimore, MD, 21224, USA.
authors.forename: Soumita
authors.initials: S
authors.lastname: Ghosh
authors.affiliation: Department of Chemical Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai, 400005, India.|Department of Systems Pharmacology and Translational Sciences, University of Pennlsylvania, 3400 Civic Center Boulevard, Philadelphia, PA, 19104, USA.
authors.forename: Arjun
authors.initials: A
authors.lastname: Sengupta
authors.affiliation: Department of Chemical Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai, 400005, India. kousikchandra@gmail.com.|Indian Institute of Science, Bangalore, 560012, India. kousikchandra@gmail.com.|Institute of Organic Chemistry, Johannes Kepler Universität Linz, Altenberger Straße 69, TNF Turm, 3rd floor, 4040, Linz, Austria. kousikchandra@gmail.com.
authors.forename: Kousik
authors.initials: K
authors.lastname: Chandra
mesh_terms: D000597:Amino Acids, Branched-Chain / Q000097:blood*; D000818:Animals; D005260:Female; D008279:Magnetic Resonance Imaging / Q000379:methods*; D055432:Metabolomics / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D000597:Amino Acids, Branched-Chain
keywords: BCAA; COSY; CPMG; Metabolites; NMR