title: Quantitative isoform-profiling of highly diversified recognition molecules.
abstract: Complex biological systems rely on cell surface cues that govern cellular self-recognition and selective interactions with appropriate partners. Molecular diversification of cell surface recognition molecules through DNA recombination and complex alternative splicing has emerged as an important principle for encoding such interactions. However, the lack of tools to specifically detect and quantify receptor protein isoforms is a major impediment to functional studies. We here developed a workflow for targeted mass spectrometry by selected reaction monitoring that permits quantitative assessment of highly diversified protein families. We apply this workflow to dissecting the molecular diversity of the neuronal neurexin receptors and uncover an alternative splicing-dependent recognition code for synaptic ligands.
authors.affiliation: Biozentrum, University of Basel, Basel, Switzerland.
authors.forename: Dietmar
authors.initials: D
authors.lastname: Schreiner
authors.affiliation: Biozentrum, University of Basel, Basel, Switzerland.
authors.forename: Jovan
authors.initials: J
authors.lastname: Simicevic
authors.affiliation: Biozentrum, University of Basel, Basel, Switzerland.
authors.forename: Erik
authors.initials: E
authors.lastname: Ahrné
authors.affiliation: Biozentrum, University of Basel, Basel, Switzerland.
authors.forename: Alexander
authors.initials: A
authors.lastname: Schmidt
authors.affiliation: Biozentrum, University of Basel, Basel, Switzerland.
authors.forename: Peter
authors.identifier: http://orcid.org/0000-0002-9516-9399
authors.initials: P
authors.lastname: Scheiffele
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D015816:Cell Adhesion Molecules, Neuronal / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D008565:Membrane Proteins / Q000737:chemistry*; D051379:Mice; D009474:Neurons / Q000737:chemistry*; D020033:Protein Isoforms / Q000737:chemistry*
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D008565:Membrane Proteins; D020033:Protein Isoforms
keywords: MRM; SRM; alternative splicing; cell biology; mouse; neuroligin; neuroscience; recognition; synapse