title: Beyond the Western front: targeted proteomics and organelle abundance profiling.
abstract: The application of westerns or immunoblotting techniques for assessing the composition, dynamics, and purity of protein extracts from plant material has become common practice. While the approach is reproducible, can be readily applied and is generally considered robust, the field of plant science suffers from a lack of antibody variety against plant proteins. The development of approaches that employ mass spectrometry to enable both relative and absolute quantification of many hundreds of proteins in a single sample from a single analysis provides a mechanism to overcome the expensive impediment in having to develop antibodies in plant science. We consider it an opportune moment to consider and better develop the adoption of multiple reaction monitoring (MRM)-based analyses in plant biochemistry.
authors.affiliation: Section for Plant Glycobiology, Department of Plant and Environmental Sciences, University of Copenhagen , Frederiksberg, Denmark.
authors.forename: Harriet T
authors.initials: HT
authors.lastname: Parsons
authors.affiliation: Joint BioEnergy Institute, Physical Biosciences Division, Lawrence Berkeley National Laboratory , Berkeley, CA, USA ; The Australian Research Council Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne , Melbourne, VIC, Australia.
authors.forename: Joshua L
authors.initials: JL
authors.lastname: Heazlewood
keywords: Arabidopsis; immunoblotting; multiple reaction monitoring (MRM); organelle abundance; proteomics; quantitative proteomics