title: Automated high-content morphological analysis of muscle fiber histology.
abstract: In the search for a cure for many muscular disorders it is often necessary to analyze muscle fibers under a microscope. For this morphological analysis, we developed an image processing approach to automatically analyze and quantify muscle fiber images so as to replace today's less accurate and time-consuming manual method. Muscular disorders, that include cardiomyopathy, muscular dystrophies, and diseases of nerves that affect muscles such as neuropathy and myasthenia gravis, affect a large percentage of the population and, therefore, are an area of active research for new treatments. In research, the morphological features of muscle fibers play an important role as they are often used as biomarkers to evaluate the progress of underlying diseases and the effects of potential treatments. Such analysis involves assessing histopathological changes of muscle fibers as indicators for disease severity and also as a criterion in evaluating whether or not potential treatments work. However, quantifying morphological features is time-consuming, as it is usually performed manually, and error-prone. To replace this standard method, we developed an image processing approach to automatically detect and measure the cross-sections of muscle fibers observed under microscopy that produces faster and more objective results. As such, it is well-suited to processing the large number of muscle fiber images acquired in typical experiments, such as those from studies with pre-clinical models that often create many images. Tests on real images showed that the approach can segment and detect muscle fiber membranes and extract morphological features from highly complex images to generate quantitative results that are readily available for statistical analysis.
authors.affiliation: Institute of Physics at Sao Carlos, University of Sao Paulo, Sao Carlos, SP, Brazil; Department of Computer Science, Midwestern State University, Guarapuava, PR, Brazil.
authors.forename: Mauro
authors.initials: M
authors.lastname: Miazaki
authors.affiliation: Institute of Physics at Sao Carlos, University of Sao Paulo, Sao Carlos, SP, Brazil.
authors.forename: Matheus P
authors.initials: MP
authors.lastname: Viana
authors.affiliation: Department of Anesthesia, Brigham and Women's Hospital, Boston, MA, USA; Department of Clinical Hematology, Southwestern Hospital, The Third Military Medical University, Chongqing, China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Institute of Physics at Sao Carlos, University of Sao Paulo, Sao Carlos, SP, Brazil.
authors.forename: Cesar H
authors.initials: CH
authors.lastname: Comin
authors.affiliation: Department of Anesthesia, Brigham and Women's Hospital, Boston, MA, USA.
authors.forename: Yaming
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Physics at Sao Carlos, University of Sao Paulo, Sao Carlos, SP, Brazil; National Institute of Science and Technology for Complex Systems, Niteroi, RJ, Brazil.
authors.forename: Luciano
authors.initials: L
authors.lastname: da F Costa
authors.affiliation: Department of Radiology, Brigham and Women's Hospital, 20 Shattuck Street, Boston, MA 02115, USA. Electronic address: xxu@bwh.harvard.edu.
authors.forename: Xiaoyin
authors.initials: X
authors.lastname: Xu
mesh_terms: D000818:Animals; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008297:Male; D051379:Mice; D018101:Mice, Inbred mdx; D018485:Muscle Fibers, Skeletal / Q000473:pathology*; D009135:Muscular Diseases / Q000473:pathology*
keywords: Cross sections; Morphology of muscle fibers; Muscular dystrophy; Quantification; Segmentation