title: Enzymatic Catalysis Combining the Breath Figures and Layer-by-Layer Techniques: Toward the Design of Microreactors.
abstract: Herein, we report the fabrication of microstructured porous surfaces with controlled enzymatic activity by combining the breath figures and the layer-by-layer techniques. Two different types of porous surfaces were designed based on fluorinated and carboxylated copolymers in combination with PS, using poly(2,3,4,5,6-pentafluorostyrene)-b-polystyrene (PS5F31-b-PS21) and polystyrene-b-poly(acrylic acid) (PS19-b-PAA10) block copolymers, respectively. For comparative purposes, flat surfaces having similar chemistry were obtained by spin-coating. Poly(sodium 4-styrenesulfonate)/poly(allylamine hydrochloride) (PSS/PAH) multilayers incorporating alkaline phosphatase (ALP) were built on these porous surfaces to localize the enzyme both inside and outside of the pores using PS/PS5F31-b-PS21 surfaces and only inside the pores on PS/PS19-b-PAA10 surfaces. A higher catalytic activity of ALP (about three times) was obtained with porous surfaces compared to the flat ones. The catalysis happens specifically inside the holes of PS/PS19-b-PAA10surfaces, where ALP is located. This opens the route for applications in microreactors.
authors.affiliation: †Instituto de Ciencia y Tecnología de Polímeros (ICTP), Consejo Superior de Investigaciones Científicas (CSIC), C/Juan de la Cierva 3, 28006 Madrid, Spain.
authors.forename: A S
authors.initials: AS
authors.lastname: De León
authors.affiliation: ‡Institut Charles Sadron, Centre National de la Recherche Scientifique, Université de Strasbourg, UPR 22, 23 rue du Loess, BP 84047, 67034, Strasbourg Cedex 2, France.
authors.forename: T
authors.initials: T
authors.lastname: Garnier
authors.affiliation: ‡Institut Charles Sadron, Centre National de la Recherche Scientifique, Université de Strasbourg, UPR 22, 23 rue du Loess, BP 84047, 67034, Strasbourg Cedex 2, France.|§Institute of Advanced Study, University of Strasbourg, 5 allée du Général Rouvillois, 67083 Strasbourg, France.|⊥Ecole de Chimie, Polymères et Matériaux, Université de Strasbourg, 25 rue Becquerel, 67087 Strasbourg, France.
authors.forename: L
authors.initials: L
authors.lastname: Jierry
authors.affiliation: ‡Institut Charles Sadron, Centre National de la Recherche Scientifique, Université de Strasbourg, UPR 22, 23 rue du Loess, BP 84047, 67034, Strasbourg Cedex 2, France.|§Institute of Advanced Study, University of Strasbourg, 5 allée du Général Rouvillois, 67083 Strasbourg, France.
authors.forename: F
authors.initials: F
authors.lastname: Boulmedais
authors.affiliation: #Departamento de Química-Física Aplicada, Facultad de Ciencias, Universidad Autónoma de Madrid, C/Francisco Tomás y Valiente 7, Cantoblanco, 28049 Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Muñoz-Bonilla
authors.affiliation: †Instituto de Ciencia y Tecnología de Polímeros (ICTP), Consejo Superior de Investigaciones Científicas (CSIC), C/Juan de la Cierva 3, 28006 Madrid, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Rodríguez-Hernández
mesh_terms: D000469:Alkaline Phosphatase / Q000737:chemistry*; D000499:Allylamine / Q000737:chemistry*; D019149:Bioreactors; D002384:Catalysis; D006801:Humans; D010316:Particle Size; D011108:Polymers / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*; D016062:Porosity; D013499:Surface Properties
chemical_list: D011108:Polymers; D011137:Polystyrenes; D000499:Allylamine; D000469:Alkaline Phosphatase
keywords: alkaline phosphatase; breath figures; catalysis of porous supports; enzymatic catalysis; microreactor design; multilayers; polyelectrolytes; surface modification; surface-confinement