title: Binding of angiogenesis inhibitor kringle 5 to its specific ligands by frontal affinity chromatography.
abstract: The interactions between angiogenesis inhibitor Kringle 5 and its five specific ligands were investigated by frontal affinity chromatography in combination with fluorescence spectra and site-directed molecular docking. The binding constants of trans-4-(aminomethyl) cyclohexane carboxylic acid (AMCHA), epsilon-aminocaproic acid (EACA), benzylamine, 7-aminoheptanoic acid (7-AHA) and L-lysine to Kringle 5 were 19.0×10(3), 7.97×10(3), 6.45×10(3), 6.07×10(3) and 4.04×10(3) L/mol, respectively. The five ligands bound to Kringle 5 on the lysine binding site in equimolar amounts, which was pushed mainly by hydrogen bond and Van der Waals force. This binding affinity was believed to be dependent on the functional group and flexible feature in ligands. This study will provide an important insight into the binding mechanism of angiogenesis inhibitor Kringle 5 to its specific ligands.
authors.affiliation: College of Life Sciences, Northwest University, Xi'an 710069, China. Electronic address: bianliujiao@sohu.com.
authors.forename: Liujiao
authors.initials: L
authors.lastname: Bian
authors.affiliation: College of Life Sciences, Northwest University, Xi'an 710069, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.affiliation: College of Life Sciences, Northwest University, Xi'an 710069, China; Department of Medicine, Xizang University for Nationalities, Xianyang 712082, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Ji
mesh_terms: D020533:Angiogenesis Inhibitors / Q000378:metabolism; D001665:Binding Sites; D002846:Chromatography, Affinity; D006860:Hydrogen Bonding; D018082:Kringles / Q000502:physiology*; D008024:Ligands; D008239:Lysine / Q000737:chemistry; D011485:Protein Binding
chemical_list: D020533:Angiogenesis Inhibitors; D008024:Ligands; D008239:Lysine
keywords: Angiogenesis inhibitor Kringle 5; Binding constant; Frontal affinity chromatography; Ligand; Lysine binding sites