title: A procedure for the preparation and isolation of nucleoside-5'-diphosphates.
abstract: Tris[bis(triphenylphosphoranylidene)ammonium] pyrophosphate (PPN pyrophosphate) was used in the SN2 displacements of the tosylate ion from 5'-tosylnucleosides to afford nucleoside-5'-diphosphates. Selective precipitation permitted the direct isolation of nucleoside-5'-diphosphates from crude reaction mixtures.
authors.affiliation: Department of Chemistry, Science Laboratories, Durham University, South Road, Durham, DH1 3LE, United Kingdom, ; Department of Chemistry, University of Turku, Vatselankatu 2, 20014 Turku, Finland.
authors.forename: Heidi J
authors.initials: HJ
authors.lastname: Korhonen
authors.affiliation: Department of Chemistry, Science Laboratories, Durham University, South Road, Durham, DH1 3LE, United Kingdom.
authors.forename: Hannah L
authors.initials: HL
authors.lastname: Bolt
authors.affiliation: Department of Chemistry, Science Laboratories, Durham University, South Road, Durham, DH1 3LE, United Kingdom.
authors.forename: David R W
authors.initials: DR
authors.lastname: Hodgson
keywords: NDP synthesis; nucleic acids; nucleoside-5’-diphosphate; phosphorylation