title: Cleavable ester-linked magnetic nanoparticles for labeling of solvent-exposed primary amine groups of peptides/proteins.
abstract: To study the solvent-exposed lysine residues of peptides/proteins, we previously reported disulfide-linked N-hydroxysuccinimide ester-modified silica-coated iron oxide magnetic nanoparticles (NHS-SS-SiO2@Fe3O4 MNPs). The presence of a disulfide bond in the linker limits the use of disulfide reducing agent during protein digestion and allows unwanted disulfide formation between the MNPs and protein. In the current work, the disulfide bond was replaced with a cleavable ester group to synthesize NHS ester-modified SiO2@Fe3O4 MNPs. Use of the cleavable ester group provides an improved method for protein labeling and allows the use of disulfide reducing agents during protein digestion.
authors.affiliation: Department of Chemistry, University of New Orleans, New Orleans, LA 70148, USA.
authors.forename: Ujwal S
authors.initials: US
authors.lastname: Patil
authors.affiliation: Department of Chemistry, University of New Orleans, New Orleans, LA 70148, USA.
authors.forename: Laura
authors.initials: L
authors.lastname: Osorno
authors.affiliation: Department of Chemistry, University of New Orleans, New Orleans, LA 70148, USA.
authors.forename: Angela
authors.initials: A
authors.lastname: Ellender
authors.affiliation: Southern Regional Research Center, New Orleans, LA 70124, USA.
authors.forename: Casey
authors.initials: C
authors.lastname: Grimm
authors.affiliation: Department of Chemistry, University of New Orleans, New Orleans, LA 70148, USA. Electronic address: mtarr@uno.edu.
authors.forename: Matthew A
authors.initials: MA
authors.lastname: Tarr
mesh_terms: D000588:Amines / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D004952:Esters; D007782:Lactoglobulins / Q000737:chemistry*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D012997:Solvents / Q000737:chemistry*; D013194:Staining and Labeling; D013696:Temperature
chemical_list: D000588:Amines; D004952:Esters; D007782:Lactoglobulins; D058185:Magnetite Nanoparticles; D010455:Peptides; D011506:Proteins; D012997:Solvents; D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide
keywords: Core shell magnetic nanoparticles; Ethylene glycol bis(succinimidylsuccinate); Solvent-exposed lysine residues