title: HPLC-UV method validation for the identification and quantification of bioactive amines in commercial eggs.
abstract: A quantitative and confirmatory high-performance liquid chromatography with ultraviolet detection (HPLC-UV) method for the determination of bioactive amines in the albumen and yolk of commercial eggs was developed, optimized and validated by analyte extraction with trichloroacetic acid and pre-column derivatization with dansyl chloride. Phenylethylamine, putrescine, cadaverine, histamine, tyramine, spermidine and spermine standards were used to evaluate the following performance parameters: limit of detection (LoD), limit of quantification (LoQ), selectivity, linearity, precision, recovery and ruggedness. The LoD of the method was defined from 0.2 to 0.3 mg kg(-1) for the yolk matrix and from 0.2 to 0.4 mg kg(-1) for the albumen matrix; the LoQ was from 0.7 to 1.0 mg kg(-1) for the yolk matrix and from 0.7 to 1.1 mg kg(-1) for the albumen matrix. The validated method exhibited excellent selectivity and separation of all amines with coefficients of determination higher than 0.99. The obtained recovery values were from 90.5% to 108.3%, and the relative standard deviation (RSD) was lower than 10% under repeatability conditions for the studied analytes. The performance parameters show the validated method to be adequate for the determination of bioactive amines in egg albumen and yolk.
authors.affiliation: Escola de Veterinária, Universidade Federal de Minas Gerais (UFMG), Avenida Antônio Carlos, 6627, Belo Horizonte 30.123-970, Brazil.
authors.forename: Tadeu Chaves
authors.initials: TC
authors.lastname: de Figueiredo
authors.affiliation: Escola de Veterinária, Universidade Federal de Minas Gerais (UFMG), Avenida Antônio Carlos, 6627, Belo Horizonte 30.123-970, Brazil.
authors.forename: Débora Cristina Sampaio
authors.initials: DC
authors.lastname: de Assis
authors.affiliation: Instituto Mineiro de Agropecuária (IMA), Rodovia Américo Gianetti, 4001, Belo Horizonte 31.630-901, Brazil.
authors.forename: Liliane Denize Miranda
authors.initials: LD
authors.lastname: Menezes
authors.affiliation: Escola de Veterinária, Universidade Federal de Minas Gerais (UFMG), Avenida Antônio Carlos, 6627, Belo Horizonte 30.123-970, Brazil.
authors.forename: Guilherme Resende
authors.initials: GR
authors.lastname: da Silva
authors.affiliation: Escola de Veterinária, Universidade Federal de Minas Gerais (UFMG), Avenida Antônio Carlos, 6627, Belo Horizonte 30.123-970, Brazil.
authors.forename: Isabela Pereira
authors.initials: IP
authors.lastname: Lanza
authors.affiliation: Laboratório de Imunologia Aplicada, Fundação Ezequiel Dias (FUNED), Rua Conde Pereira Carneiro, 80, Belo Horizonte 30.510-010, Brazil.
authors.forename: Luiz Guilherme Dias
authors.initials: LG
authors.lastname: Heneine
authors.affiliation: Escola de Veterinária, Universidade Federal de Minas Gerais (UFMG), Avenida Antônio Carlos, 6627, Belo Horizonte 30.123-970, Brazil. Electronic address: silvanavc@ufmg.br.
authors.forename: Silvana de Vasconcelos
authors.initials: Sde V
authors.lastname: Cançado
mesh_terms: D000418:Albumins / Q000737:chemistry*; D001679:Biogenic Amines / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004530:Egg Yolk / Q000737:chemistry*; D010627:Phenethylamines / Q000032:analysis*
chemical_list: D000418:Albumins; D001679:Biogenic Amines; D010627:Phenethylamines
keywords: Bioactive amines; Eggs; HPLC–UV; Validation