title: Functionalizing Microporous Membranes for Protein Purification and Protein Digestion.
abstract: This review examines advances in the functionalization of microporous membranes for protein purification and the development of protease-containing membranes for controlled protein digestion prior to mass spectrometry analysis. Recent studies confirm that membranes are superior to bead-based columns for rapid protein capture, presumably because convective mass transport in membrane pores rapidly brings proteins to binding sites. Modification of porous membranes with functional polymeric films or TiO₂ nanoparticles yields materials that selectively capture species ranging from phosphopeptides to His-tagged proteins, and protein-binding capacities often exceed those of commercial beads. Thin membranes also provide a convenient framework for creating enzyme-containing reactors that afford control over residence times. With millisecond residence times, reactors with immobilized proteases limit protein digestion to increase sequence coverage in mass spectrometry analysis and facilitate elucidation of protein structures. This review emphasizes the advantages of membrane-based techniques and concludes with some challenges for their practical application.
authors.affiliation: Department of Chemistry, Michigan State University, East Lansing, Michigan 48824; email: bruening@chemistry.msu.edu.
authors.forename: Jinlan
authors.initials: J
authors.lastname: Dong
authors.forename: Merlin L
authors.initials: ML
authors.lastname: Bruening
mesh_terms: D000818:Animals; D006801:Humans; D008567:Membranes, Artificial; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000302:isolation & purification* / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D008567:Membranes, Artificial; D011108:Polymers; D011506:Proteins
keywords: His-tagged proteins; layer-by-layer adsorption; membrane adsorbers; phosphopeptide enrichment; polymer brushes; protease reactors