title: Occurrence of Babesia species in captive reindeer (Rangifer tarandus) in Germany.
abstract: Two cases of acute babesiosis in captive reindeer (Rangifer tarandus) in two German zoos in 2009 and 2012 triggered this study to investigate the occurrence and species diversity of Babesia parasites infecting reindeer in different zoos and deer parks in Germany. Between June and December 2013, blood samples were taken from 123 clinically inapparent reindeer from 16 different facilities. Samples were tested for the presence of Babesia species DNA by conventional PCR and sequence analysis of part of the 18S rRNA gene. Also, Giemsa-stained smears of reindeer blood samples were examined for parasitaemia by light microscopy. The overall PCR-prevalence in blood samples was 23.6% (n=29). Comparison of sequenced amplicons with GenBank entries possibly revealed up to five different Babesia species: B. venatorum (n=19), B. capreoli (n=2) and B. capreoli-like (n=4), B. odocoilei-like (n=2) and B. divergens (n=1), while one sample turned out to be a Theileria sp. Out of the 16 facilities in the study, 12 housed at least one positive animal. In Giemsa-stained blood smears, intra-erythrocytic Babesia parasites were detected in samples of three reindeer from three locations. The high prevalence of Babesia infections implicates babesiosis to be a relevant infectious disease threat for captive reindeer in Germany. Consequently, reindeer with clinical signs compatible to those of acute babesiosis should either be tested for the presence of Babesia spp. DNA or blood smears should be examined for parasitaemia.
authors.affiliation: Institute for Animal Hygiene and Veterinary Public Heath, Veterinary Faculty, University of Leipzig, An den Tierkliniken 1, D-04103 Leipzig, Germany; Zoo Duisburg AG, Mülheimer Str. 273, D-47058 Duisburg, Germany.
authors.forename: Lisa
authors.initials: L
authors.lastname: Wiegmann
authors.affiliation: Institute of Comparative Tropical Medicine and Parasitology, Ludwig-Maximilians-Universität München, Leopoldstr. 5, D-80802 Munich, Germany; Institute of Parasitology, National Reference Center of Vector Entomology, University of Zurich, Winterthurerstr. 266a, CH-8057 Zurich, Switzerland.
authors.forename: Cornelia
authors.initials: C
authors.lastname: Silaghi
authors.affiliation: Institute for Animal Hygiene and Veterinary Public Heath, Veterinary Faculty, University of Leipzig, An den Tierkliniken 1, D-04103 Leipzig, Germany.
authors.forename: Anna
authors.initials: A
authors.lastname: Obiegala
authors.affiliation: Institute for Animal Hygiene and Veterinary Public Heath, Veterinary Faculty, University of Leipzig, An den Tierkliniken 1, D-04103 Leipzig, Germany.
authors.forename: Carolin
authors.initials: C
authors.lastname: Karnath
authors.affiliation: Zoo Duisburg AG, Mülheimer Str. 273, D-47058 Duisburg, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Langer
authors.affiliation: Zoo Duisburg AG, Mülheimer Str. 273, D-47058 Duisburg, Germany.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Ternes
authors.affiliation: Tierpark Cottbus, Kiekebuscher Str. 5, D-03042 Cottbus, Germany.
authors.forename: Jens
authors.initials: J
authors.lastname: Kämmerling
authors.affiliation: Zoo Dortmund, Mergelteichstr. 80, D-44225 Dortmund, Germany.
authors.forename: Christine
authors.initials: C
authors.lastname: Osmann
authors.affiliation: Institute for Animal Hygiene and Veterinary Public Heath, Veterinary Faculty, University of Leipzig, An den Tierkliniken 1, D-04103 Leipzig, Germany. Electronic address: pfeffer@vetmed.uni-leipzig.de.
authors.forename: Martin
authors.initials: M
authors.lastname: Pfeffer
mesh_terms: D000818:Animals; D001403:Babesia / Q000235:genetics / Q000302:isolation & purification*; D001404:Babesiosis / Q000453:epidemiology* / Q000469:parasitology; D001483:Base Sequence; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005260:Female; D005858:Germany / Q000453:epidemiology; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012053:Reindeer / Q000469:parasitology*; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal
keywords: Babesia spp.; Germany; Reindeer