title: Primary human nasal epithelial cell response to titanium surface with a nanonetwork structure in nasal implant applications.
abstract: In nasal reconstruction applications, the response of cells to titanium (Ti) implants is largely determined by the surface characteristics of the implant. This study investigated an electrochemical anodization surface treatment intended to improve the response of primary human nasal epithelial cells (HNEpC) to Ti surfaces in nasal implant applications. We used a simple and fast electrochemical anodization treatment, i.e., applying anodic current, to produce a titanium dioxide (TiO2) nanonetwork layer on the Ti surface with average lateral pore size below 100 nm, depending on the current applied. The TiO2 nanonetwork layer exhibited enhanced hydrophilicity and protein adsorption ability compared with untreated Ti surfaces. In addition, the spreading morphology, cytoskeletal arrangement, and proliferation of HNEpC on the nanonetwork layer indicated excellent cell response characteristics. This research advances our understanding regarding the means by which a TiO2 nanonetwork layer can improve the response of HNEpC to Ti surfaces in nasal implant applications.
authors.affiliation: Institute of Oral Biology, National Yang-Ming University, No.155, Sec.2, Linong Street, Taipei, 112 Taiwan.
authors.forename: Wei-En
authors.initials: WE
authors.lastname: Yang
authors.affiliation: Department of Otolaryngology, Taipei Veterans General Hospital, No.201, Sec.2, Shipai Road, Taipei, 112 Taiwan ; School of Medicine, National Yang-Ming University, No.155, Sec.2, Linong Street, Taipei, 112 Taiwan.
authors.forename: Ming-Ying
authors.initials: MY
authors.lastname: Lan
authors.affiliation: Institute of Materials Science and Engineering, National Central University, No. 300, Jhongda Road, Taoyuan, 320 Taiwan.
authors.forename: Sheng-Wei
authors.initials: SW
authors.lastname: Lee
authors.affiliation: Institute of Materials Science and Engineering, National Central University, No. 300, Jhongda Road, Taoyuan, 320 Taiwan.
authors.forename: Jeng-Kuei
authors.initials: JK
authors.lastname: Chang
authors.affiliation: Institute of Oral Biology, National Yang-Ming University, No.155, Sec.2, Linong Street, Taipei, 112 Taiwan ; Department of Dentistry, National Yang-Ming University, No.155, Sec.2, Linong Street, Taipei, 112 Taiwan ; Graduate Institute of Basic Medical Science, China Medical University, No.91, Hsueh-Shih Road, Taichung, 404 Taiwan ; Department of Medical Research, China Medical University Hospital, No.2, Yude Road, Taichung, 404 Taiwan ; Department of Biomedical Informatics, Asia University, No.500, Lioufeng Road, Taichung, 413 Taiwan ; Department of Stomatology, Taipei Veterans General Hospital, No.201, Sec.2, Shipai Road, Taipei, 112 Taiwan.
authors.forename: Her-Hsiung
authors.initials: HH
authors.lastname: Huang
keywords: Cell response; Electrochemical anodization; Human nasal epithelial cell; Nanonetwork; Titanium nasal implant