title: High yields of fatty acid and neutral lipid production from cassava bagasse hydrolysate (CBH) by heterotrophic Chlorella protothecoides.
abstract: The fermentation process for high yields of fatty acid and neutral lipid production from cassava bagasse hydrolysate (CBH) was developed by heterotrophic Chlorella protothecoides. An efficient single-step enzymatic hydrolysis of cassava bagasse (CB) by cellulase was firstly developed to produce >30 g/L of reducing sugars. The concentrated CBH was subsequently applied in a batch culture, producing 7.9 g/L of dry biomass with yield of 0.44 g/g reducing sugar and 34.3 wt% of fatty acids and 48.6 wt% of neutral lipids. Furthermore, fed-batch fermentation using CBH achieved higher yields of fatty acids (41.0 wt% and a titer of 5.83 g/L) and neutral lipids (58.4 wt% and yield of 0.22 g/g reducing sugar). Additionally, the fatty acid profile analysis showed that the intercellular lipid was suitable to prepare high-quality biodiesel. This study demonstrated the feasibility of using CBH as low-cost feedstock to produce crude algal oil for sustainable biodiesel production.
authors.affiliation: School of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, PR China.
authors.forename: Junhui
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Chemical and Biological Engineering, The University of Alabama, Tuscaloosa, AL 35487, USA. Electronic address: mliu@eng.ua.edu.
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Liu
authors.affiliation: School of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, PR China. Electronic address: fewd304@scut.edu.cn.
authors.forename: Dong
authors.initials: D
authors.lastname: Wei
authors.affiliation: School of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, PR China.
authors.forename: Gu
authors.initials: G
authors.lastname: Chen
mesh_terms: D002708:Chlorella / Q000378:metabolism*; D005227:Fatty Acids / Q000096:biosynthesis*; D005285:Fermentation; D006868:Hydrolysis; D008055:Lipids / Q000096:biosynthesis*; D002365:Manihot / Q000378:metabolism*
chemical_list: D005227:Fatty Acids; D008055:Lipids
keywords: Cassava bagasse hydrolysate; Chlorella protothecoides; Fatty acid; Fed-batch fermentation; Neutral lipid