title: A fluorescent tool set for yeast Atg proteins.
abstract: Fluorescence microscopy of live cells is instrumental in deciphering the molecular details of autophagy. To facilitate the routine examination of yeast Atg proteins under diverse conditions, here we provide a comprehensive tool set, including (1) plasmids for the expression of GFP chimeras at endogenous levels for most Atg proteins, (2) RFP-Atg8 constructs with improved properties as a PAS marker, and (3) plasmids for the complementation of common yeast auxotrophic markers. We hope that the availability of this tool set will further accelerate yeast autophagy research.
authors.affiliation: a School of Life Sciences and Biotechnology; Shanghai Jiao Tong University ; Shanghai , China.
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Jing-Zhen
authors.initials: JZ
authors.lastname: Song
authors.forename: Mei-Hua
authors.initials: MH
authors.lastname: Shan
authors.forename: Shi-Ping
authors.initials: SP
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Zhu
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.forename: Jianping
authors.initials: J
authors.lastname: Lin
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Xie
mesh_terms: D000818:Animals; D001343:Autophagy / Q000502:physiology*; D000071190:Autophagy-Related Protein 8 Family; D000071183:Autophagy-Related Proteins; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D010588:Phagosomes / Q000378:metabolism; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014617:Vacuoles / Q000378:metabolism
chemical_list: C113757:ATG8 protein, S cerevisiae; D000071190:Autophagy-Related Protein 8 Family; D000071183:Autophagy-Related Proteins; D008869:Microtubule-Associated Proteins; D029701:Saccharomyces cerevisiae Proteins; D049452:Green Fluorescent Proteins; D011494:Protein Kinases; C477823:ATG1 protein, S cerevisiae
keywords: Atg, autophagy related; C,G,R,YFP, cyan, green, red and yellow fluorescent protein; Cvt, cytoplasm-to-vacuole targeting; DsRed eExpress 2; PAS, phagophore assembly site; Vps, vacuolar protein sorting.; autophagy; auxotroph; fluorescent protein; mKO, monomeric Kusabira Orange; pseudo-monomer; starter kit; yeast