title: Cellular response of chondrocytes to magnesium alloys for orthopedic applications.
abstract: In the present study, the effects of Mg-Nd-Zn-Zr (JDBM), brushite (CaHPO4·2H2O)-coated JDBM (C-JDBM), AZ31, WE43, pure magnesium (Mg) and Ti alloy (TC4) on rabbit chondrocytes were investigated in vitro. Adhesion experiments revealed the satisfactory morphology of chondrocytes on the surface of all samples. An indirect cytotoxicity test using MTT assay revealed that C‑JDBM and TC4 exhibited results similar to those of the negative control, better than those obtained with JDBM, AZ31, WE43 and pure Mg (p<0.05). There were no statistically significant differences observed between the JDBM, AZ31, WE43 and pure Mg group (p>0.05). The results of indirect cell cytotoxicity and proliferation assays, as well as those of apoptosis assay, glycosaminoglycan (GAG) quantification, assessment of collagen Ⅱ (Col Ⅱ) levels and RT-qPCR revealed a similar a trend as was observed with MTT assay. These findings suggested that the JDBM alloy was highly biocompatible with chondrocytes in vitro, yielding results similar to those of AZ31, WE43 and pure Mg. Furthermore, CaHPO4·2H2O coating significantly improved the biocompatibility of this alloy.
authors.affiliation: Department of Orthopaedics, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, P.R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liao
authors.affiliation: Department of Orthopaedics, The Huashan Hospital Baoshan Branch, Fudan University, Shanghai 200431, P.R. China.
authors.forename: Qingli
authors.initials: Q
authors.lastname: Xu
authors.affiliation: National Engineering Research Center of Light Alloys Net Forming (LAF), School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, P.R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: National Engineering Research Center of Light Alloys Net Forming (LAF), School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, P.R. China.
authors.forename: Jialing
authors.initials: J
authors.lastname: Niu
authors.affiliation: National Engineering Research Center of Light Alloys Net Forming (LAF), School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, P.R. China.
authors.forename: Guangyin
authors.initials: G
authors.lastname: Yuan
authors.affiliation: Department of Orthopaedics, The Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, P.R. China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Orthopaedics, The Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, P.R. China.
authors.forename: Yaohua
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Radiology, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, P.R. China.
authors.forename: Xinling
authors.initials: X
authors.lastname: Wang
mesh_terms: D000497:Alloys / Q000494:pharmacology; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002130:Calcium Phosphates / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000502:physiology*; D007719:Knee Joint / Q000166:cytology; D008274:Magnesium / Q000494:pharmacology*; D017616:Magnesium Compounds / Q000494:pharmacology; D008422:Materials Testing; D019637:Orthopedic Procedures; D011817:Rabbits
chemical_list: D000497:Alloys; D002130:Calcium Phosphates; D017616:Magnesium Compounds; D008274:Magnesium; C494366:calcium phosphate, dibasic, dihydrate