title: Generation and characterization of osteochondral grafts with human nasal chondrocytes.
abstract: We investigated whether nasal chondrocytes (NC) can be used to generate composite constructs with properties necessary for the repair of osteochondral (OC) lesions, namely maturation, integration and capacity to recover from inflammatory burst. OC grafts were fabricated by combining engineered cartilage tissues (generated by culturing NC or articular chondrocytes - AC - onto Chondro-Gide® matrices) with devitalized spongiosa cylinders (Tutobone®). OC tissues were then exposed to IL-1β for three days and cultured for additional 2 weeks in the absence of IL-1β. Cartilage maturation extent was assessed (immune) histologically, biochemically and by delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) while cartilage/bone integration was assessed using a peel-off mechanical test. The use of NC as compared to AC allowed for more efficient cartilage matrix accumulation and superior integration of the cartilage/bone layers. dGEMRIC and biochemical analyzes of the OC constructs showed a reduced glycosaminoglycan (GAG) contents upon IL-1β administration. Cartilaginous matrix contents and integration forces returned to baseline up on withdrawal of IL-1β. By having a cartilage layer well developed and strongly integrated to the subchondral layer, OC tissues generated with NC may successfully engraft in an inflammatory post-surgery joint environment.
authors.affiliation: Departments of Surgery and of Biomedicine, Basel University Hospital, University of Basel, Basel, Switzerland.
authors.forename: Marina
authors.initials: M
authors.lastname: Barandun
authors.affiliation: Departments of Surgery and of Biomedicine, Basel University Hospital, University of Basel, Basel, Switzerland.
authors.forename: Lukas Daniel
authors.initials: LD
authors.lastname: Iselin
authors.affiliation: Department of Radiology, Clinic of Radiology and Nuclear Medicine, University of Basel Hospital, Basel, Switzerland.
authors.forename: Francesco
authors.initials: F
authors.lastname: Santini
authors.affiliation: Department of Radiology, Clinic of Radiology and Nuclear Medicine, University of Basel Hospital, Basel, Switzerland.
authors.forename: Michele
authors.initials: M
authors.lastname: Pansini
authors.affiliation: I.R.C.C.S., Istituto Ortopedico Galeazzi, Milano, Italy.
authors.forename: Celeste
authors.initials: C
authors.lastname: Scotti
authors.affiliation: Bone Tumor Reference Center at the Institute of Pathology, Basel University Hospital, Basel, Switzerland.
authors.forename: Daniel
authors.initials: D
authors.lastname: Baumhoer
authors.affiliation: Department of Radiology, Clinic of Radiology and Nuclear Medicine, University of Basel Hospital, Basel, Switzerland.
authors.forename: Oliver
authors.initials: O
authors.lastname: Bieri
authors.affiliation: Department of Radiology, Clinic of Radiology and Nuclear Medicine, University of Basel Hospital, Basel, Switzerland.
authors.forename: Ueli
authors.initials: U
authors.lastname: Studler
authors.affiliation: Laboratory for Biomechanics and Biocalorimetry, Biozentrum- Pharmazentrum, University of Basel, Basel, Switzerland.|Orthomerian, postCode, 4054, Basel, Switzerland.
authors.forename: Dieter
authors.initials: D
authors.lastname: Wirz
authors.affiliation: Departments of Surgery and of Biomedicine, Basel University Hospital, University of Basel, Basel, Switzerland.
authors.forename: Martin
authors.initials: M
authors.lastname: Haug
authors.affiliation: Departments of Surgery and of Biomedicine, Basel University Hospital, University of Basel, Basel, Switzerland.
authors.forename: Marcel
authors.initials: M
authors.lastname: Jakob
authors.affiliation: Departments of Surgery and of Biomedicine, Basel University Hospital, University of Basel, Basel, Switzerland.
authors.forename: Dirk Johannes
authors.initials: DJ
authors.lastname: Schaefer
authors.affiliation: Departments of Surgery and of Biomedicine, Basel University Hospital, University of Basel, Basel, Switzerland.
authors.forename: Ivan
authors.initials: I
authors.lastname: Martin
authors.affiliation: Departments of Surgery and of Biomedicine, Basel University Hospital, University of Basel, Basel, Switzerland.
authors.forename: Andrea
authors.initials: A
authors.lastname: Barbero
mesh_terms: D000368:Aged; D002358:Cartilage, Articular / Q000166:cytology; D019902:Chondrocytes / Q000637:transplantation*; D005260:Female; D006025:Glycosaminoglycans / Q000032:analysis; D006801:Humans; D053583:Interleukin-1beta / Q000494:pharmacology; D008279:Magnetic Resonance Imaging; D008297:Male; D008875:Middle Aged; D009666:Nose / Q000166:cytology*; D023822:Tissue Engineering
chemical_list: D006025:Glycosaminoglycans; D053583:Interleukin-1beta
keywords: chondrocytes; chondrogenic differentiation; dGEMRIC; osteochondral lesion; tissue engineering