title: Simultaneous topographic and recognition imaging of epidermal growth factor receptor (EGFR) on single human breast cancer cells.
abstract: Epidermal growth factor receptor (EGFR) plays an important role in signaling pathway of the development of breast cancer cells. Since EGFR overexpresses in most breast cancer cells, it is regarded as a biomarker molecule of breast cancer cells. Here we demonstrated a new AFM technique-topography and recognition (TREC) imaging-to simultaneously obtain highly sensitive and specific molecular recognition images and high-resolution topographic images of EGFR on single breast cancer cells.
authors.affiliation: Department of Biological Engineering, Utah State University, Logan , UT 84322-4105, USA.
authors.forename: Lifu
authors.initials: L
authors.lastname: Xiao
authors.affiliation: Department of Biological Engineering, Utah State University, Logan , UT 84322-4105, USA.
authors.forename: Qian
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Department of Biological Engineering, Utah State University, Logan , UT 84322-4105, USA.
authors.forename: Yangzhe
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Computer Science, Utah State University, Logan, UT 84322-4205, USA.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Qi
authors.affiliation: Department of Biological Engineering, Utah State University, Logan , UT 84322-4105, USA. Electronic address: Anhong.Zhou@usu.edu.
authors.forename: Anhong
authors.initials: A
authors.lastname: Zhou
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000378:metabolism*; D066246:ErbB Receptors / Q000378:metabolism*; D005260:Female; D006801:Humans; D018625:Microscopy, Atomic Force / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D025941:Protein Interaction Mapping / Q000379:methods; D013347:Subcellular Fractions / Q000378:metabolism / Q000473:pathology
chemical_list: D004815:Epidermal Growth Factor; D066246:ErbB Receptors
keywords: Atomic force microscopy (AFM); Epidermal growth factor receptor (EGFR); Topography and RECognition (TREC) imaging