title: Diverse activity of human secretory phospholipases A2 on the migration of human vascular smooth muscle cells.
abstract: OBJECTIVE
  Investigation of the diversity of human secretory phospholipases A2 (sPLA2) on the migration of human vascular smooth muscle cells (VSMC).
  
  
  METHODS
  We investigated the impact of sPLA2 IIA, V, and X and of oleic acid, linoleic acid and lysophosphatidylcholine on the migration of human VSMC.
  
  
  METHODS
  Recombinant human sPLA2's and Boyden's chamber method were applied.
  
  
  RESULTS
  sPLA2, IIA but not V or X enhanced migration of VSMC in a dose/time dependent manner. Oleic and linoleic acids, and lysophosphatidylcholine markedly enhanced migration.
  
  
  CONCLUSIONS
  These results imply that sPLA2 IIA, which is known to be present in the arterial wall in the vicinity of VSMC, as well as products of lipid hydrolysis induced by sPLA2, enhance the migration of VSMC, and thus may contribute to atherogenic process.
authors.affiliation: Department of Medicine, University of Toronto, Toronto, Canada, drwpruzanski@bellnet.ca.
authors.forename: W
authors.initials: W
authors.lastname: Pruzanski
authors.forename: J
authors.initials: J
authors.lastname: Kopilov
authors.forename: A
authors.initials: A
authors.lastname: Kuksis
mesh_terms: D002465:Cell Movement / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000494:pharmacology; D019787:Linoleic Acid / Q000494:pharmacology; D008244:Lysophosphatidylcholines / Q000494:pharmacology; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000187:drug effects*; D032389:Myocytes, Smooth Muscle / Q000187:drug effects*; D019301:Oleic Acid / Q000494:pharmacology; D054497:Phospholipases A2, Secretory / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D007527:Isoenzymes; D008244:Lysophosphatidylcholines; D011994:Recombinant Proteins; D019301:Oleic Acid; D019787:Linoleic Acid; D054497:Phospholipases A2, Secretory