title: A New Fractionation and Recovery Method of Viral Genomes Based on Nucleic Acid Composition and Structure Using Tandem Column Chromatography.
abstract: Metagenomic studies have revealed the unexplored diversity of the environmental virosphere. However, most studies are biased towards specific types of viral genomes due to the absence of universal methods to access all viral genome types. In the present study, we established a novel system to efficiently separate single- and double-stranded DNA/RNA viral genomes using hydroxyapatite and cellulose chromatography. This method will allow us to quantitatively and simultaneously access four types of viral genomes and will provide important clues to further understand previously unexplored environmental viral populations and obtain potentially unbiased libraries from environmental viral communities.
authors.affiliation: Research and Development Center for Marine Biosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC).
authors.forename: Syun-Ichi
authors.initials: S
authors.lastname: Urayama
authors.forename: Yukari
authors.initials: Y
authors.lastname: Yoshida-Takashima
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Yoshida
authors.forename: Yuji
authors.initials: Y
authors.lastname: Tomaru
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Moriyama
authors.forename: Ken
authors.initials: K
authors.lastname: Takai
authors.forename: Takuro
authors.initials: T
authors.lastname: Nunoura
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D016679:Genome, Viral; D056186:Metagenomics / Q000379:methods*; D009696:Nucleic Acids / Q000737:chemistry* / Q000302:isolation & purification*; D014773:Virology / Q000379:methods*; D014780:Viruses / Q000235:genetics* / Q000302:isolation & purification*
chemical_list: D009696:Nucleic Acids