title: Multitarget PCR Assay for Direct Detection of Penicillinase-Producing Neisseria gonorrhoeae for Enhanced Surveillance of Gonococcal Antimicrobial Resistance.
abstract: A multitarget PCR was developed for the direct detection of penicillinase-producing Neisseria gonorrhoeae (PPNG). The assay was validated by testing 342 PPNG isolates and 415 clinical samples. The method is suitable for routine detection of PPNG strains. Its multitarget approach reduces the potential for false-negative results caused by sequence variations.
authors.affiliation: Queensland Paediatric Infectious Diseases Laboratory, Queensland Children's Medical Research Institute, Queensland, Australia The University of Queensland, Brisbane, Queensland, Australia.
authors.forename: Cameron
authors.initials: C
authors.lastname: Buckley
authors.affiliation: Queensland Paediatric Infectious Diseases Laboratory, Queensland Children's Medical Research Institute, Queensland, Australia The University of Queensland, Brisbane, Queensland, Australia.
authors.forename: Ella
authors.initials: E
authors.lastname: Trembizki
authors.affiliation: Microbiology Laboratory, Pathology Department, Royal Darwin Hospital, Darwin, Northern Territory, Australia.
authors.forename: Robert W
authors.initials: RW
authors.lastname: Baird
authors.affiliation: Melbourne Sexual Health Centre, Alfred Health, Carlton Victoria, Australia Central Clinical School, Monash University, Victoria, Australia.
authors.forename: Marcus
authors.initials: M
authors.lastname: Chen
authors.affiliation: Kirby Institute, University of New South Wales, Sydney, NSW, Australia Sydney Sexual Health Centre, Sydney Hospital, Sydney, NSW, Australia.
authors.forename: Basil
authors.initials: B
authors.lastname: Donovan
authors.affiliation: Microbiology Laboratory, Pathology Department, Royal Darwin Hospital, Darwin, Northern Territory, Australia.
authors.forename: Kevin
authors.initials: K
authors.lastname: Freeman
authors.affiliation: WHO Collaborating Centre for STD, Microbiology Department, South Eastern Area Laboratory Services, Prince of Wales Hospital, Sydney, New South Wales, Australia Nepean Clinical School, University of Sydney, Kingswood, New South Wales, Australia.
authors.forename: Namraj
authors.initials: N
authors.lastname: Goire
authors.affiliation: Kirby Institute, University of New South Wales, Sydney, NSW, Australia.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Guy
authors.affiliation: WHO Collaborating Centre for STD, Microbiology Department, South Eastern Area Laboratory Services, Prince of Wales Hospital, Sydney, New South Wales, Australia.
authors.forename: Monica M
authors.initials: MM
authors.lastname: Lahra
authors.affiliation: Kirby Institute, University of New South Wales, Sydney, NSW, Australia.
authors.forename: David
authors.initials: D
authors.lastname: Regan
authors.affiliation: Queensland Paediatric Infectious Diseases Laboratory, Queensland Children's Medical Research Institute, Queensland, Australia The University of Queensland, Brisbane, Queensland, Australia d.whiley@uq.edu.au.
authors.forename: David M
authors.initials: DM
authors.lastname: Whiley
mesh_terms: D024881:Drug Resistance, Bacterial; D062665:Epidemiological Monitoring; D006069:Gonorrhea / Q000453:epidemiology / Q000382:microbiology*; D006801:Humans; D009344:Neisseria gonorrhoeae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D010405:Penicillinase / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D010405:Penicillinase