title: Biocompatibility of poly(2-alkyl-2-oxazoline) brush surfaces for adherent lung cell lines.
abstract: Development of synthetic surfaces that are highly reproducible and biocompatible for in vitro cell culture offers potential for development of improved models for studies of cellular physiology and pathology. They may also be useful in tissue engineering by removal of the need for biologically-derived components such as extracellular matrix proteins. We synthesised four types of 2-alkyl-2-oxazoline polymers ranging from the hydrophilic poly(2-methyl-2-oxazoline) to the hydrophobic poly(2-n-butyl-2-oxazoline). The polymers were terminated using amine-functionalised glass coverslips, enabling the synthetic procedure to be reproducible and scaleable. The polymer-coated glass slides were tested for biocompatibility using human epithelial (16HBE14o-) and fibroblastic (MRC5) cell lines. Differences in adhesion and motility of the two cell types was observed, with the poly(2-isopropyl-2-oxazoline) polymer equally supporting the growth of both cell types, whereas poly(2-n-butyl-2-oxazoline) showed selectivity for fibroblast growth. In summary, 2-alkyl-2-oxazoline polymers may be a useful tool for building in vitro model cell culture models with preferential adhesion of specific cell types.
authors.affiliation: Brooke Laboratories, Clinical and Experimental Sciences and the Southampton NIHR Respiratory Biomedical Research Unit, Faculty of Medicine, University of Southampton, University Hospital Southampton NHS Foundation Trust, Southampton, UK.
authors.forename: Angela
authors.initials: A
authors.lastname: Tait
authors.affiliation: Department of Chemistry, Faculty of Natural and Environmental Sciences, University of Southampton, Southampton, UK.
authors.forename: Adam L
authors.initials: AL
authors.lastname: Fisher
authors.affiliation: Department of Chemistry, Faculty of Natural and Environmental Sciences, University of Southampton, Southampton, UK.
authors.forename: Tom
authors.initials: T
authors.lastname: Hartland
authors.affiliation: Brooke Laboratories, Clinical and Experimental Sciences and the Southampton NIHR Respiratory Biomedical Research Unit, Faculty of Medicine, University of Southampton, University Hospital Southampton NHS Foundation Trust, Southampton, UK.
authors.forename: David
authors.initials: D
authors.lastname: Smart
authors.affiliation: Engineering Science, Faculty of Engineering and the Environment, University of Southampton, Southampton, UK; Institute for Life Sciences, University of Southampton, Southampton, UK.
authors.forename: Peter
authors.initials: P
authors.lastname: Glynne-Jones
authors.affiliation: Engineering Science, Faculty of Engineering and the Environment, University of Southampton, Southampton, UK; Institute for Life Sciences, University of Southampton, Southampton, UK.
authors.forename: Martyn
authors.initials: M
authors.lastname: Hill
authors.affiliation: Brooke Laboratories, Clinical and Experimental Sciences and the Southampton NIHR Respiratory Biomedical Research Unit, Faculty of Medicine, University of Southampton, University Hospital Southampton NHS Foundation Trust, Southampton, UK; Institute for Life Sciences, University of Southampton, Southampton, UK.
authors.forename: Emily J
authors.initials: EJ
authors.lastname: Swindle
authors.affiliation: Department of Chemistry, Faculty of Natural and Environmental Sciences, University of Southampton, Southampton, UK.
authors.forename: Martin
authors.initials: M
authors.lastname: Grossel
authors.affiliation: Brooke Laboratories, Clinical and Experimental Sciences and the Southampton NIHR Respiratory Biomedical Research Unit, Faculty of Medicine, University of Southampton, University Hospital Southampton NHS Foundation Trust, Southampton, UK; Institute for Life Sciences, University of Southampton, Southampton, UK. Electronic address: D.E.Davies@southampton.ac.uk.
authors.forename: Donna E
authors.initials: DE
authors.lastname: Davies
mesh_terms: D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects*; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D006801:Humans; D008168:Lung / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D008422:Materials Testing; D010080:Oxazoles / Q000138:chemical synthesis* / Q000494:pharmacology*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D010080:Oxazoles
keywords: Biocompatibility; Cell adhesion; Cell proliferation; Co-culture; Lung; Synthetic polymer