title: Evaluation of Gestational Diabetes Mellitus Risk in South Indian Women Based on MTHFR (C677T) and FVL (G1691A) Mutations.
abstract: We aimed to scrutinize the extent to which single amino acid substitutions in the MTHFR and factor V Leiden (FVL) genes affect the risk of gestational diabetes mellitus (GDM) in pregnant women of South Indian descendant. This case-control study was implemented once the ethical approval has been obtained. Overall, 237 women were recruited in this study: 137 had been diagnosed with GDM and the remaining 100 women were used as normal controls or non-GDM. The diagnosis of GDM was confirmed with biochemical analysis, i.e., GCT and oral glucose tolerance tests. Five milliliters of peripheral blood was collected and used for biochemical and molecular analyses. DNA was isolated, and genotyping for MTHFR (C677T) and FVL (G1691A) mutations was performed using PCR-RFLP. FVL (G1691A) locus was not polymorphic in the investigated sample. There was no significant difference in the allele and genotype frequencies of C677T polymorphism between GDM and non-GDM women (p = 0.8892).
authors.affiliation: Department of Genetics and Molecular Medicine, Kamineni Hospitals , Hyderabad , India ; Department of Genetics, Vasavi Medical and Research Center , Hyderabad , India ; Department of Genetics and Biotechnology, Osmania University , Hyderabad , India.
authors.forename: Imran Ali
authors.initials: IA
authors.lastname: Khan
authors.affiliation: Department of Genetic Medicine, Faculty of Medicine, King Abdulaziz University , Jeddah , Saudi Arabia.
authors.forename: Noor Ahmad
authors.initials: NA
authors.lastname: Shaik
authors.affiliation: Department of Gynecology and Obstetrics, Kamineni Hospitals , Hyderabad , India.
authors.forename: Vasundhara
authors.initials: V
authors.lastname: Kamineni
authors.affiliation: Department of Genetics and Biotechnology, Osmania University , Hyderabad , India.
authors.forename: Parveen
authors.initials: P
authors.lastname: Jahan
authors.affiliation: Department of Genetics and Molecular Medicine, Kamineni Hospitals , Hyderabad , India ; Department of Genetics, Vasavi Medical and Research Center , Hyderabad , India.
authors.forename: Qurratulain
authors.initials: Q
authors.lastname: Hasan
authors.affiliation: Department of Biochemistry, Kasturba Medical College, Manipal University , Manipal Karnataka , India.
authors.forename: Pragna
authors.initials: P
authors.lastname: Rao
keywords: C677T; FVL; G1691A; MTHFR; gestational diabetes mellitus