title: Lanostane-type triterpenoids from Abies faxoniana and their DNA topoisomerase inhibitory activities.
abstract: Nine lanostane-type triterpenoids were isolated from branches and leaves of Abies faxoniana, along with 10 known compounds. Two were isolated as inseparable mixtures of epimers at C-23 of the γ-lactone ring that had a lactol structure. The structures of the nine compounds were established by spectroscopic analysis and circular dichroism (CD) data. The absolute configurations at the stereogenic centres of two of the known compounds were confirmed by X-ray crystallography. One compound showed cytotoxic activities against HCT-116, MCF-7, and A549 cells with IC50 values of 8.9, 7.6, and 4.2μM, respectively. The isolated compounds were tested for their effects on human DNA topoisomerases I and II. One was found to be a selective inhibitor of human topo II activity with an IC50 value of 53.5μM, which was comparable to that of the topo II inhibitor etoposide (IC50=49.6μM).
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, PR China; College of Pharmaceutical Sciences and Chinese Medicine, Southwest University, Chongqing 400715, PR China.
authors.forename: Guo-Wei
authors.initials: GW
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fujian 350108, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Lv
authors.affiliation: Department of Phytochemistry, Second Military Medical University, Shanghai 200433, PR China.
authors.forename: Xing
authors.initials: X
authors.lastname: Yuan
authors.affiliation: Department of Phytochemistry, Second Military Medical University, Shanghai 200433, PR China.
authors.forename: Ji
authors.initials: J
authors.lastname: Ye
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, PR China.
authors.forename: Hui-Zi
authors.initials: HZ
authors.lastname: Jin
authors.affiliation: Department of Phytochemistry, Second Military Medical University, Shanghai 200433, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Shan
authors.affiliation: Department of Phytochemistry, Second Military Medical University, Shanghai 200433, PR China.
authors.forename: Xi-Ke
authors.initials: XK
authors.lastname: Xu
authors.affiliation: Department of Phytochemistry, Second Military Medical University, Shanghai 200433, PR China. Electronic address: shenyunheng@hotmail.com.
authors.forename: Yun-Heng
authors.initials: YH
authors.lastname: Shen
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, PR China; Department of Phytochemistry, Second Military Medical University, Shanghai 200433, PR China. Electronic address: wdzhangy@hotmail.com.
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Zhang
mesh_terms: D028202:Abies / Q000737:chemistry*; D018360:Crystallography, X-Ray; D026942:DNA Topoisomerases / Q000187:drug effects*; D006801:Humans; D020128:Inhibitory Concentration 50; D007810:Lanosterol / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D015394:Molecular Structure; D035261:Plant Components, Aerial / Q000737:chemistry; D013237:Stereoisomerism; D059005:Topoisomerase II Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D059003:Topoisomerase Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D059005:Topoisomerase II Inhibitors; D059003:Topoisomerase Inhibitors; D007810:Lanosterol; D026942:DNA Topoisomerases
keywords: Abies faxoniana; Pinaceae; Topoisomerases; Triterpenoids