title: Adjuvant-dependent immunogenicity of Staphylococcus aureus Efb and Map proteins in chickens.
abstract: The avian IgY antibodies generated in hens and isolated from egg yolk have gained in popularity as they present an alternative source of antibodies for diagnostic as well as therapeutic applications. One of the advantages of IgY technology are the large amounts of produced antibodies from a single animal combined with their high reactivity representing an attractive alternative for mammalian antibodies. Despite many known protocols for the immunization of chickens, the administration of new antigens often requires additional modification such as antigen dose or use of an adjuvant in order to elicit a significant immune response. We investigated the immunogenicity of three Staphylococcus aureus antigens including two extracellular proteins Map and Efb and one selected Efb105-124 epitope conjugated to KLH that were administered to the animals. Additionally, the immunization protocol included two adjuvant systems: Freund's complete adjuvant and Emulsigen-D. The results demonstrated a high immunostimulatory potency of Freund's complete adjuvant, especially in case of Efb compared to the immune response elicited by Emulsigen-D. However, after immunization with the KLH-Efb105-124 conjugate, the obtained antibodies showed similar reactivity regardless of adjuvant system used with the only exception being their avidity.
authors.affiliation: Wroclaw University of Technology, Faculty of Chemistry, Division of Medicinal Chemistry and Microbiology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland.
authors.forename: Renata
authors.initials: R
authors.lastname: Grzywa
authors.affiliation: Wroclaw University of Technology, Faculty of Chemistry, Division of Medicinal Chemistry and Microbiology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Walczak
authors.affiliation: Wroclaw University of Technology, Faculty of Chemistry, Division of Medicinal Chemistry and Microbiology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Łupicka-Słowik
authors.affiliation: Wroclaw University of Environmental and Life Sciences, Faculty of Veterinary Medicine, Department of Epizootiology and Clinic of Bird and Exotic Animals, Pl. Grunwaldzki 45, 50-366 Wroclaw, Poland.
authors.forename: Kamila
authors.initials: K
authors.lastname: Bobrek
authors.affiliation: European Molecular Biology Laboratory (EMBL), Notkestraße 85 c/o DESY, Building 25A, 22603 Hamburg, Germany.
authors.forename: Stephane
authors.initials: S
authors.lastname: Boivin
authors.affiliation: Center for Infectious Diseases, University of Texas School of Public Health, Houston, TX 77030, USA.
authors.forename: Eric L
authors.initials: EL
authors.lastname: Brown
authors.affiliation: Wroclaw University of Environmental and Life Sciences, Faculty of Veterinary Medicine, Department of Epizootiology and Clinic of Bird and Exotic Animals, Pl. Grunwaldzki 45, 50-366 Wroclaw, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Gaweł
authors.affiliation: Wroclaw University of Environmental and Life Sciences, Faculty of Veterinary Medicine, Department of Immunology, Pathophysiology and Veterinary Preventive Medicine, Norwida 31, 50-375 Wroclaw, Poland.
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Stefaniak
authors.affiliation: Wroclaw University of Technology, Faculty of Chemistry, Division of Medicinal Chemistry and Microbiology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland.
authors.forename: Józef
authors.initials: J
authors.lastname: Oleksyszyn
authors.affiliation: Wroclaw University of Technology, Faculty of Chemistry, Division of Medicinal Chemistry and Microbiology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland. Electronic address: marcin.sienczyk@pwr.edu.pl.
authors.forename: Marcin
authors.initials: M
authors.lastname: Sieńczyk
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology*; D002645:Chickens / Q000276:immunology*; D005260:Female; D007114:Immunization; D007136:Immunoglobulins / Q000096:biosynthesis
chemical_list: D000276:Adjuvants, Immunologic; D001426:Bacterial Proteins; C114014:Efb protein, Staphylococcus aureus; C016512:IgY; D007136:Immunoglobulins; C095564:Map protein, Staphylococcus aureus
keywords: Adjuvant; Extracellular fibrinogen binding protein; Immunoglobulin Y; MHC class II analog protein; Staphylococcus aureus