title: Evaluation of antimicrobial activity of extracts of in vivo and in vitro grown Vinca rosea L. (Catharanthus roseus) against pathogens.
abstract: The antimicrobial activity of Vinca rosea was evaluated against pathogenic bacterial strains (Bacillus subtilis, B. licheniformis and Azotobacter sp.) and fungal strains (Asprgillus niger, Alternaria solani and Rhizopus oryzae) using agar well diffusion method. Methanolic extracts of in vivo leaf, in vitro leaf, in vitro calluses of leaf, nodal and fruit explants were used and exhibited antimicrobial activity as indicated by minimum inhibitory concentration (MIC). In vitro extracts showed better results as compared to the in vivo extracts for both the antibacterial as well as the antifungal activity. Among all the extracts, maximum zone of inhibition (30.3 mm ± 0.58(a)) was formed by in vitro leaf callus extract concentration of 2.0mg/ml against B. licheniformis. Similarly in case of antifungal activity, maximum zone of inhibition (34.6mm ± 0.57(a)) was formed by in vitro leaf callus extract and MIC value is 6.0mg/ml against A. niger. Hence these results clearly depicts that V. rosea possess a great strength to fight against the microbial activity and can be used against various infections.
authors.affiliation: Department of Biotechnology and Microbiology, Lahore College for Women University, Lahore, Pakistan.
authors.forename: Shagufta
authors.initials: S
authors.lastname: Naz
authors.affiliation: Department of Biotechnology and Microbiology, Lahore College for Women University, Lahore, Pakistan.
authors.forename: Rukhama
authors.initials: R
authors.lastname: Haq
authors.affiliation: Department of Biotechnology and Microbiology, Lahore College for Women University, Lahore, Pakistan.
authors.forename: Farah
authors.initials: F
authors.lastname: Aslam
authors.affiliation: Department of Biotechnology and Microbiology, Lahore College for Women University, Lahore, Pakistan.
authors.forename: Saiqa
authors.initials: S
authors.lastname: Ilyas
mesh_terms: D000528:Alternaria / Q000187:drug effects; D000890:Anti-Infective Agents / Q000494:pharmacology*; D001234:Aspergillus niger / Q000187:drug effects; D001395:Azotobacter / Q000187:drug effects*; D001407:Bacillus / Q000187:drug effects; D001412:Bacillus subtilis / Q000187:drug effects*; D030008:Catharanthus; D005658:Fungi / Q000187:drug effects*; D066298:In Vitro Techniques; D008826:Microbial Sensitivity Tests; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000494:pharmacology*; D012233:Rhizopus / Q000187:drug effects
chemical_list: D000890:Anti-Infective Agents; D010936:Plant Extracts