title: Vitamin B12 and omega-3 fatty acids together regulate lipid metabolism in Wistar rats.
abstract: Our recent study indicates that maternal vitamin B12 and omega-3 fatty acid status influence plasma and erythrocyte fatty acid profile in dams. The present study examines the effects of prenatal and postnatal vitamin B12 and omega-3 fatty acid status on lipid metabolism in the offspring. Pregnant dams were divided into five groups: Control; Vitamin B12 deficient (BD); Vitamin B12 supplemented (BS); Vitamin B12 deficient group supplemented with omega-3 fatty acids (BDO); Vitamin B12 supplemented group with omega-3 fatty acids (BSO). The offspring were continued on the same diets till 3 month of age. Vitamin B12 deficiency increased cholesterol levels (p<0.01) but reduced docosahexaenoic acid (DHA) (p<0.05), liver mRNA levels of acetyl CoA carboxylase-1 (ACC-1) (p<0.05) and carnitine palmitoyltransferase-1 (CPT-1) (p<0.01) in the offspring. Omega-3 fatty acid supplementation to this group normalized cholesterol but not mRNA levels of ACC-1 and CPT-1. Vitamin B12 supplementation normalized the levels cholesterol to that of control but increased plasma triglyceride (p<0.01) and reduced liver mRNA levels of adiponectin, ACC-1, and CPT-1 (p<0.01 for all). Supplementation of both vitamin B12 and omega-3 fatty acid normalized triglyceride and mRNA levels of all the above genes. Prenatal and postnatal vitamin B12 and omega-3 fatty acids together play a crucial role in regulating the genes involved in lipid metabolism in adult offspring.
authors.affiliation: Department of Nutritional Medicine, Interactive Research School for Health Affairs, Bharati Vidyapeeth Deemed University, Pune Satara Road, Pune 411043, India.
authors.forename: Amrita
authors.initials: A
authors.lastname: Khaire
authors.affiliation: Department of Nutritional Medicine, Interactive Research School for Health Affairs, Bharati Vidyapeeth Deemed University, Pune Satara Road, Pune 411043, India.
authors.forename: Richa
authors.initials: R
authors.lastname: Rathod
authors.affiliation: Department of Nutritional Medicine, Interactive Research School for Health Affairs, Bharati Vidyapeeth Deemed University, Pune Satara Road, Pune 411043, India.
authors.forename: Anvita
authors.initials: A
authors.lastname: Kale
authors.affiliation: Department of Nutritional Medicine, Interactive Research School for Health Affairs, Bharati Vidyapeeth Deemed University, Pune Satara Road, Pune 411043, India. Electronic address: srjoshi62@gmail.com.
authors.forename: Sadhana
authors.initials: S
authors.lastname: Joshi
mesh_terms: D052242:Adiponectin / Q000378:metabolism; D000818:Animals; D001794:Blood Pressure; D002784:Cholesterol / Q000097:blood; D004032:Diet; D019587:Dietary Supplements; D015525:Fatty Acids, Omega-3 / Q000097:blood / Q000378:metabolism*; D005260:Female; D006710:Homocysteine / Q000097:blood; D050356:Lipid Metabolism; D008099:Liver / Q000737:chemistry / Q000502:physiology; D008297:Male; D011247:Pregnancy; D039401:Prenatal Nutritional Physiological Phenomena; D012333:RNA, Messenger / Q000378:metabolism; D017208:Rats, Wistar; D014280:Triglycerides / Q000097:blood; D014805:Vitamin B 12 / Q000097:blood / Q000378:metabolism*
chemical_list: D052242:Adiponectin; D015525:Fatty Acids, Omega-3; D012333:RNA, Messenger; D014280:Triglycerides; D006710:Homocysteine; D002784:Cholesterol; D014805:Vitamin B 12
keywords: Acetyl CoA carboxylase-1; Adiponectin; Carnitine palmitoyltransferase-1; Omega-3 fatty acids; Triglycerides; Vitamin B(12)