title: Complete genome sequence and integrated protein localization and interaction map for alfalfa dwarf virus, which combines properties of both cytoplasmic and nuclear plant rhabdoviruses.
abstract: We have determined the full-length 14,491-nucleotide genome sequence of a new plant rhabdovirus, alfalfa dwarf virus (ADV). Seven open reading frames (ORFs) were identified in the antigenomic orientation of the negative-sense, single-stranded viral RNA, in the order 3'-N-P-P3-M-G-P6-L-5'. The ORFs are separated by conserved intergenic regions and the genome coding region is flanked by complementary 3' leader and 5' trailer sequences. Phylogenetic analysis of the nucleoprotein amino acid sequence indicated that this alfalfa-infecting rhabdovirus is related to viruses in the genus Cytorhabdovirus. When transiently expressed as GFP fusions in Nicotiana benthamiana leaves, most ADV proteins accumulated in the cell periphery, but unexpectedly P protein was localized exclusively in the nucleus. ADV P protein was shown to have a homotypic, and heterotypic nuclear interactions with N, P3 and M proteins by bimolecular fluorescence complementation. ADV appears unique in that it combines properties of both cytoplasmic and nuclear plant rhabdoviruses.
authors.affiliation: Instituto de Patología Vegetal (IPAVE), Centro de Investigaciones Agropecuarias (CIAP), Instituto Nacional de Tecnología Agropecuaria (INTA), Camino a 60 Cuadras k 5,5, Córdoba X5020ICA, Argentina; Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, QLD 4072, Australia. Electronic address: n.bejerman@uq.edu.au.
authors.forename: Nicolás
authors.initials: N
authors.lastname: Bejerman
authors.affiliation: Instituto de Patología Vegetal (IPAVE), Centro de Investigaciones Agropecuarias (CIAP), Instituto Nacional de Tecnología Agropecuaria (INTA), Camino a 60 Cuadras k 5,5, Córdoba X5020ICA, Argentina.
authors.forename: Fabián
authors.initials: F
authors.lastname: Giolitti
authors.affiliation: Instituto de Patología Vegetal (IPAVE), Centro de Investigaciones Agropecuarias (CIAP), Instituto Nacional de Tecnología Agropecuaria (INTA), Camino a 60 Cuadras k 5,5, Córdoba X5020ICA, Argentina.
authors.forename: Soledad
authors.initials: S
authors.lastname: de Breuil
authors.affiliation: Instituto de Patología Vegetal (IPAVE), Centro de Investigaciones Agropecuarias (CIAP), Instituto Nacional de Tecnología Agropecuaria (INTA), Camino a 60 Cuadras k 5,5, Córdoba X5020ICA, Argentina.
authors.forename: Verónica
authors.initials: V
authors.lastname: Trucco
authors.affiliation: Instituto de Patología Vegetal (IPAVE), Centro de Investigaciones Agropecuarias (CIAP), Instituto Nacional de Tecnología Agropecuaria (INTA), Camino a 60 Cuadras k 5,5, Córdoba X5020ICA, Argentina.
authors.forename: Claudia
authors.initials: C
authors.lastname: Nome
authors.affiliation: Instituto de Patología Vegetal (IPAVE), Centro de Investigaciones Agropecuarias (CIAP), Instituto Nacional de Tecnología Agropecuaria (INTA), Camino a 60 Cuadras k 5,5, Córdoba X5020ICA, Argentina.
authors.forename: Sergio
authors.initials: S
authors.lastname: Lenardon
authors.affiliation: Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, QLD 4072, Australia.
authors.forename: Ralf G
authors.initials: RG
authors.lastname: Dietzgen
mesh_terms: D002467:Cell Nucleus / Q000737:chemistry; D016000:Cluster Analysis; D003593:Cytoplasm / Q000737:chemistry; D021901:DNA, Intergenic; D016679:Genome, Viral; D000455:Medicago sativa / Q000821:virology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D060066:Protein Interaction Maps; D012367:RNA, Viral / Q000235:genetics; D012209:Rhabdoviridae / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000821:virology; D014764:Viral Proteins / Q000032:analysis*
chemical_list: D021901:DNA, Intergenic; D012367:RNA, Viral; D014764:Viral Proteins
keywords: Cytorhabdovirus; Genome structure; Nucleorhabdovirus; Phosphoprotein; Protein localization; Protein–protein interactions