title: 6-OHDA-Induced Changes in Parkinson's Disease-Related Gene Expression are not Affected by the Overexpression of PGAM5 in In Vitro Differentiated Embryonic Mesencephalic Cells.
abstract: LUHMES cells, a recently established line of immortalized embryonic mesencephalic cells, are the novel in vitro model for studying Parkinson's disease (PD) and dopaminergic neuron biology. Phosphoglyceromutase 5 (PGAM5) is a mitochondrial protein involved in mitophagy, mitochondria dynamics, and other processes important for PD pathogenesis. We tested the impact of lentiviral overexpression of PGAM5 protein in LUHMES cells on their differentiation and expression of 84 PD-related genes. LUHMES cells were transduced with PGAM5 or mock and treated with 100 μM 6-hydroxydopamine (6-OHDA), a model PD neurotoxin. Real-Time PCR analysis revealed that the treatment with 6-OHDA-induced changes in expression of 44 PD-related genes. PGAM5 transduction alone did not cause alternations in PD-related genes expression, nor it affected changes in gene expression mediated by 6-OHDA. The 6-OHDA-induced PD-related gene expression profile of LUHMES cells is presented for the first time and widely discussed.
authors.affiliation: Centre for Radiobiology and Biological Dosimetry, Institute of Nuclear Chemistry and Technology, Dorodna 16, 03-195, Warsaw, Poland. t.stepkowski@ichtj.waw.pl.
authors.forename: Tomasz Maciej
authors.initials: TM
authors.lastname: Stępkowski
authors.affiliation: Centre for Radiobiology and Biological Dosimetry, Institute of Nuclear Chemistry and Technology, Dorodna 16, 03-195, Warsaw, Poland.
authors.forename: Iwona
authors.initials: I
authors.lastname: Wasyk
authors.affiliation: Department of Molecular Biophysics, University of Łódź, Banacha 12/16, 90-237, Łódź, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Grzelak
authors.affiliation: Centre for Radiobiology and Biological Dosimetry, Institute of Nuclear Chemistry and Technology, Dorodna 16, 03-195, Warsaw, Poland.|Department of Molecular Biology and Translational Research, Institute of Rural Health, Jaczewskiego 2, 20-090, Lublin, Poland.|Department of Medical Biology and Translational Research, Faculty of Medicine, University of Information Technology and Management, Sucharskiego 2, 35-225, Rzeszów, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Kruszewski
mesh_terms: D002352:Carrier Proteins / Q000096:biosynthesis*; D002454:Cell Differentiation / Q000187:drug effects* / Q000502:physiology; D002461:Cell Line, Transformed; D005786:Gene Expression Regulation; D006801:Humans; D008636:Mesencephalon / Q000187:drug effects* / Q000196:embryology / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000096:biosynthesis*; D016627:Oxidopamine / Q000633:toxicity*; D020734:Parkinsonian Disorders / Q000139:chemically induced / Q000378:metabolism*; D010749:Phosphoprotein Phosphatases
chemical_list: D002352:Carrier Proteins; D024101:Mitochondrial Proteins; D016627:Oxidopamine; C529563:PGAM5 protein, human; D010749:Phosphoprotein Phosphatases
keywords: 6-hydroxydopamine; Gene expression; LUHMES cells; PGAM5; Parkinson’s disease