title: Carbon tetrachloride-induced liver injury in mice is tissue factor dependent.
abstract: Tissue factor (TF) is a membranous glycoprotein that activates the coagulation system when blood vessels or tissues are damaged. TF was up-regulated in monocrotaline (MCT)/lipopolysaccharide (LPS) hepatotoxicity model. The present study aimed to test the hypothesis that TF-dependent fibrin deposition occurs in liver toxicity induced by CCl4 in mice. Pericentral deposition of TF and fibrin is induced after CCl4-induced liver toxicity. The toxicity was evaluated by determination of serum activities of ALT, AST and ALP as well as GSH content and histopathological changes. The results showed that injection of mice with TF-antisense deoxyoligonucleotide (TF-AS) prevented the accumulation of TF and fibrin in the hepatic tissues. Furthermore, it significantly restored blood biochemical parameters, GSH content and distorted histopathological features caused by CCl4. The current study demonstrates that TF activation is associated with CCl4-induced liver injury. Furthermore, administration of TF-AS successfully prevented this type of liver injury.
authors.affiliation: Faculty of pharmacy, Al-Azhar university, Cairo 11884 Egypt; Faculty of pharmacy, Aljouf university, Aljouf, Saudi Arabia.
authors.forename: M S
authors.initials: MS
authors.lastname: Abdel-Bakky
authors.affiliation: Faculty of pharmacy, Al-Azhar university, Cairo 11884 Egypt; Faculty of pharmacy, Nahda university, Bani Suef, Egypt.
authors.forename: G K
authors.initials: GK
authors.lastname: Helal
authors.affiliation: Faculty of pharmacy, Al-Azhar university, Cairo 11884 Egypt.
authors.forename: E M
authors.initials: EM
authors.lastname: El-Sayed
authors.affiliation: Faculty of Pharmacy, Delta University for Science and Technology, Mansoura, Egypt. Electronic address: mosa1200@yahoo.com.
authors.forename: A S
authors.initials: AS
authors.lastname: Saad
mesh_terms: D000818:Animals; D002251:Carbon Tetrachloride / Q000633:toxicity*; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology / Q000378:metabolism / Q000473:pathology*; D016373:DNA, Antisense / Q000008:administration & dosage / Q000494:pharmacology; D004195:Disease Models, Animal; D005337:Fibrin / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D008297:Male; D051379:Mice; D013925:Thromboplastin / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000637:Transaminases / Q000097:blood
chemical_list: D016373:DNA, Antisense; D005337:Fibrin; D013925:Thromboplastin; D002251:Carbon Tetrachloride; D000637:Transaminases; D005978:Glutathione
keywords: CCl(4); Fibrin; Liver injury.; Oligonucleotide; Tissue factor antisense