title: Cux2-positive radial glial cells generate diverse subtypes of neocortical projection neurons and macroglia.
abstract: We recently published genetic lineage-tracing experiments using the Fezf2 and Cux2 loci. These experiments demonstrated that at both the clonal and population levels Fezf2(+) RGCs are multipotent and that at the population level Cux2(+) RGCs are multipotent. Here, we extend our work on the lineages of Fezf2(+) and Cux2(+) RGCs. Clonal analysis of E10.5 neocortical progenitors suggests that most, if not all, Cux2(+) and Fezf2(+) RGCs generate diverse projection neuron subtypes located throughout layers 2-6. These results support our previous conclusion that both Fezf2(+) and Cux2(+) RGCs are multipotent neocortical progenitors. This Matters Arising Response paper addresses the Gil-Sanz et al. (2015) Matters Arising paper, published concurrently in Neuron.
authors.affiliation: Department of Molecular, Cell and Developmental Biology, University of California, Santa Cruz, CA 95064, USA.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Eckler
authors.affiliation: Department of Molecular, Cell and Developmental Biology, University of California, Santa Cruz, CA 95064, USA; Department of Biological Sciences, San Jose State University, San Jose, CA 95192, USA.
authors.forename: Ton D
authors.initials: TD
authors.lastname: Nguyen
authors.affiliation: Department of Molecular, Cell and Developmental Biology, University of California, Santa Cruz, CA 95064, USA.
authors.forename: William L
authors.initials: WL
authors.lastname: McKenna
authors.affiliation: Department of Molecular, Cell and Developmental Biology, University of California, Santa Cruz, CA 95064, USA.
authors.forename: Ben L
authors.initials: BL
authors.lastname: Fastow
authors.affiliation: Department of Molecular, Cell and Developmental Biology, University of California, Santa Cruz, CA 95064, USA.
authors.forename: Chao
authors.initials: C
authors.lastname: Guo
authors.affiliation: Department of Psychiatry and Nina Ireland Laboratory of Developmental Neurobiology, University of California, San Francisco, CA 95158, USA.
authors.forename: John L R
authors.initials: JLR
authors.lastname: Rubenstein
authors.affiliation: Department of Molecular, Cell and Developmental Biology, University of California, Santa Cruz, CA 95064, USA. Electronic address: bchen@ucsc.edu.
authors.forename: Bin
authors.initials: B
authors.lastname: Chen
mesh_terms: D019070:Cell Lineage / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D018398:Homeodomain Proteins / Q000378:metabolism*; D039902:Multipotent Stem Cells / Q000166:cytology; D019579:Neocortex / Q000166:cytology / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D055495:Neurogenesis / Q000502:physiology; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D009836:Oligodendroglia
chemical_list: C101672:Cux2 protein, mouse; D018398:Homeodomain Proteins; D009419:Nerve Tissue Proteins