title: Notch-1 knockdown suppresses proliferation, migration and metastasis of salivary adenoid cystic carcinoma cells.
abstract: BACKGROUND
  Notch-1 promotes invasion and metastasis of cancer cells but its role in salivary adenoid cystic carcinoma (SACC) remains unelucidated. Here, we sought to investigate the effect of Notch-1 knockdown on the invasion and metastasis of SACC cells.
  
  
  METHODS
  Stable ACC-M cells whose Notch-1 was silenced by lentiviral vectors were established. Cellular proliferation was evaluated by the MTT assays and clonogenic assays, apoptosis by flow cytometry and the migration of ACC-M cells by Transwell assays. Metastasis was evaluated by examining the number of lung nodules in Balb⁄c nu⁄nu nude mice bearing subcutaneous SACC xenografts.
  
  
  RESULTS
  Our MTT assay revealed that Notch-1 knockdown significantly suppressed the proliferation of ACC-M cells compared with non-infected or scrambled control cells. Clonogenic assays further showed that Notch-1 knockdown significantly suppressed the clonogenic growth of ACC-M cells (p < 0.01 vs. controls). Our flow cytometry demonstrated that Notch-1 knockdown was associated with a significantly higher proportion of late apoptotic and necrotic cells (p < 0.01 vs. controls). Transwell assays revealed that Notch-1 knockdown markedly reduced the migratory capacity of ACC-M cells (p < 0.01 vs. controls) and xenograft studies showed that the number of metastatic nodules in the lung surface was significantly lower in nude mice bearing xenografts with Notch-1 knockdown compared to those bearing control xenografts (p < 0.01 vs. controls).
  
  
  CONCLUSIONS
  Notch-1 knockdown suppresses the growth and migration of SACC cells in vitro and the metastasis of SACC cells in vivo. Notch-1 may be a new candidate target in SACC.
authors.affiliation: Department of Stomatology, Jinling Hospital, School of Medicine, Nanjing University, 305 East Zhongshan Road, 210002, Nanjing, Jiangsu, China. rollphy@gmail.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Stomatology, Jinling Hospital, School of Medicine, Nanjing University, 305 East Zhongshan Road, 210002, Nanjing, Jiangsu, China. caogangfmmu@sina.cn.
authors.forename: Gang
authors.initials: G
authors.lastname: Cao
authors.affiliation: Department of Immunology & Rheumatology, Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing, Jiangsu, China. biancaxinran@163.com.
authors.forename: Xinran
authors.initials: X
authors.lastname: Yuan
authors.affiliation: Department of Stomatology, Jinling Hospital, School of Medicine, Nanjing University, 305 East Zhongshan Road, 210002, Nanjing, Jiangsu, China. 779567326@qq.com.
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Stomatology, Jinling Hospital, School of Medicine, Nanjing University, 305 East Zhongshan Road, 210002, Nanjing, Jiangsu, China. qingqingzhang025@126.com.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Stomatology, Jinling Hospital, School of Medicine, Nanjing University, 305 East Zhongshan Road, 210002, Nanjing, Jiangsu, China. yinglanzhu@126.com.
authors.forename: Yinglan
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Stomatology, Jinling Hospital, School of Medicine, Nanjing University, 305 East Zhongshan Road, 210002, Nanjing, Jiangsu, China. dongzhendz@163.com.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Dong
authors.affiliation: Department of Stomatology, Jinling Hospital, School of Medicine, Nanjing University, 305 East Zhongshan Road, 210002, Nanjing, Jiangsu, China. doczhangsl@gmail.com.
authors.forename: Senlin
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis; D003528:Carcinoma, Adenoid Cystic / Q000378:metabolism* / Q000473:pathology*; D002453:Cell Cycle; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D002999:Clone Cells; D015536:Down-Regulation; D055785:Gene Knockdown Techniques; D005822:Genetic Vectors / Q000378:metabolism; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D008819:Mice, Nude; D009362:Neoplasm Metastasis; D034741:RNA, Small Interfering / Q000378:metabolism; D051880:Receptors, Notch / Q000378:metabolism*; D015203:Reproducibility of Results; D012468:Salivary Gland Neoplasms / Q000378:metabolism* / Q000473:pathology*; D014162:Transfection
chemical_list: D034741:RNA, Small Interfering; D051880:Receptors, Notch