title: Two myeloid differentiation factor 88 (MyD88) isoforms identified in ducks.
abstract: MyD88 is an adaptor protein involved in the interleukin-1 receptor-induced and Toll-like receptor (TLR)-induced activation of nuclear factor-κB (NF-κB). In this study, we identified two isoforms of MyD88 gene, designated DuMyD88-X1 and DuMyD88-X2, from duck cells. Both variants were determined to have a death domain at the N-terminal and a Toll/IL-1R (TIR) domain at the C-terminal; however, the TIR domain of DuMyD88-X2 was incomplete and was 81 amino acids shorter than DuMyD88-X1. Quantitative real-time reverse transcription PCR revealed broad expression of both MyD88s. During Newcastle disease virus (NDV) challenge experiments, expression of the two genes increased significantly, with DuMyD88-X1 having a larger amplitude and longer duration. Overexpression of DuMyD88-X1 and DuMyD88-X2 induced the activation of NF-κB and IL-6 in vitro, suggesting that DuMyD88-X1 and DuMyD88-X2 may be important in the innate immune response. The results verify the existence of a MyD88-dependent signaling pathway in ducks and contribute to understanding the potential role of MyD88s in the innate immune response.
authors.affiliation: School of Agriculture and Biology, Shanghai Key Laboratory of Veterinary Biotechnology, Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Yuqiang
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: School of Agriculture and Biology, Shanghai Key Laboratory of Veterinary Biotechnology, Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Hengan
authors.initials: H
authors.lastname: Wang
authors.affiliation: School of Agriculture and Biology, Shanghai Key Laboratory of Veterinary Biotechnology, Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Yaxian
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China. Electronic address: shoveldeen@shvri.ac.cn.
authors.forename: Chan
authors.initials: C
authors.lastname: Ding
authors.affiliation: School of Agriculture and Biology, Shanghai Key Laboratory of Veterinary Biotechnology, Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: sunjhe@sjtu.edu.cn.
authors.forename: Jianhe
authors.initials: J
authors.lastname: Sun
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030161:Avian Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004372:Ducks / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D015870:Gene Expression; D007113:Immunity, Innate; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008969:Molecular Sequence Data; D053594:Myeloid Differentiation Factor 88 / Q000235:genetics* / Q000378:metabolism; D009521:Newcastle Disease / Q000276:immunology / Q000378:metabolism; D009928:Organ Specificity; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013154:Spleen / Q000378:metabolism
chemical_list: D030161:Avian Proteins; D016207:Cytokines; D053594:Myeloid Differentiation Factor 88; D020033:Protein Isoforms
keywords: Duck; IL-6; Innate immunity; MyD88; NF-κB; Newcastle disease virus