title: A single bout of whole-leg, peristaltic pulse external pneumatic compression upregulates PGC-1α mRNA and endothelial nitric oxide sythase protein in human skeletal muscle tissue.
abstract: What is the central question of this study? Does 60 min of peristaltic pulse external pneumatic compression (EPC) alter gene and protein expression patterns related to metabolism, vascular biology, redox balance and inflammation in vastus lateralis biopsy samples? What is the main finding and its importance? A single bout of EPC transiently upregulates PGC-1α mRNA, while also upregulating endothelial nitric oxide synthase protein and nitric oxide metabolite concentrations in vastus lateralis biopsy samples. We investigated whether a single 60 min bout of whole-leg, lower pressure external pneumatic compression (EPC) altered select vascular, metabolic, antioxidant and inflammation-related mRNAs. Ten participants (eight male, two female; aged 22.0 ± 0.4 years) reported to the laboratory 4 h postprandial, and vastus lateralis muscle biopsies were obtained before (PRE) and 1 and 4 h after EPC treatment. Messenger RNA expression was analysed using real-time RT-PCR, and significant mRNA findings were investigated further by Western blot analysis of respective protein concentrations. Peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α) mRNA increased by 77% 1 h following EPC compared with PRE levels (P = 0.005), but no change in protein concentration 1 or 4 h post-EPC was observed. Increases in endothelial nitric oxide sythase (eNOS) mRNA (+44%) and superoxide dismutase 2 (SOD2) mRNA (+57%) 1 h post-EPC as well as an increase in interleukin-10 mRNA (+132%) 4 h post-EPC compared with PRE levels were observed, but only approached significance (P = 0.076, 0.077 and 0.074, respectively). Interestingly, eNOS protein (+40%, P = 0.025) and nitrate and nitrite (NOx) concentrations (+69%, P = 0.025) increased 1-4 h post-EPC. Moreover, SOD2 protein tended to increase from PRE to 4 h post-EPC (+43%, P = 0.074), although no changes in tissue 4-hydroxnonenal levels was observed. An acute bout of EPC transiently upregulates PGC-1α mRNA, while also upregulating eNOS protein and NOx concentrations in vastus lateralis biopsy samples. Future research should characterize the origin of these responses (e.g. vascular or muscle fibre cells) and how the acute effects of EPC application on gene and protein expression observed herein are associated with functional improvements (e.g. metabolism, vascular function) in acute and chronic models.
authors.affiliation: School of Kinesiology, Auburn University, Auburn, AL, USA.
authors.forename: Wesley C
authors.initials: WC
authors.lastname: Kephart
authors.affiliation: School of Kinesiology, Auburn University, Auburn, AL, USA.
authors.forename: C Brooks
authors.initials: CB
authors.lastname: Mobley
authors.affiliation: School of Kinesiology, Auburn University, Auburn, AL, USA.
authors.forename: Carlton D
authors.initials: CD
authors.lastname: Fox
authors.affiliation: School of Kinesiology, Auburn University, Auburn, AL, USA.|Department of Cell Biology and Physiology, Edward Via College of Osteopathic Medicine - Auburn Campus, Auburn, AL, USA.
authors.forename: David D
authors.initials: DD
authors.lastname: Pascoe
authors.affiliation: School of Kinesiology, Auburn University, Auburn, AL, USA.
authors.forename: JoEllen M
authors.initials: JM
authors.lastname: Sefton
authors.affiliation: The Orthopaedic Clinic, Auburn, AL, USA.
authors.forename: Trent J
authors.initials: TJ
authors.lastname: Wilson
authors.affiliation: Department of Cell Biology and Physiology, Edward Via College of Osteopathic Medicine - Auburn Campus, Auburn, AL, USA.|Athletic Department, Auburn University, Auburn, AL, USA.
authors.forename: Michael D
authors.initials: MD
authors.lastname: Goodlett
authors.affiliation: School of Kinesiology, Auburn University, Auburn, AL, USA.
authors.forename: Andreas N
authors.initials: AN
authors.lastname: Kavazis
authors.affiliation: School of Kinesiology, Auburn University, Auburn, AL, USA.|Department of Cell Biology and Physiology, Edward Via College of Osteopathic Medicine - Auburn Campus, Auburn, AL, USA.
authors.forename: Michael D
authors.initials: MD
authors.lastname: Roberts
authors.affiliation: School of Kinesiology, Auburn University, Auburn, AL, USA.|Department of Cell Biology and Physiology, Edward Via College of Osteopathic Medicine - Auburn Campus, Auburn, AL, USA.
authors.forename: Jeffrey S
authors.initials: JS
authors.lastname: Martin
mesh_terms: D005260:Female; D006801:Humans; D007866:Leg / Q000502:physiology*; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D052250:Nitric Oxide Synthase Type III / Q000235:genetics* / Q000378:metabolism; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D012333:RNA, Messenger / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D015533:Transcriptional Activation / Q000502:physiology; D015854:Up-Regulation; D055815:Young Adult
chemical_list: C486497:PPARGC1A protein, human; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D012333:RNA, Messenger; D014157:Transcription Factors; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III