title: Multiplex detection of plant pathogens through the Luminex MagPlex bead system.
abstract: Here we describe a versatile multiplex method for both the serological and molecular detection of plant pathogens. The Luminex MagPlex bead system uses small paramagnetic microspheres ("beads"), either coated with specific antibodies or oligonucleotides, which capture respectively viruses and/or bacteria or PCR products obtained from their genetic material. The Luminex MagPlex bead system allows true multiplex detection of up to 500 targets in a single sample on a routine basis. The liquid suspension nature of the method significantly improves (1) assay speed, (2) detection limits and (3) dynamic range. It can also considerably reduce labor and consumables costs.
authors.affiliation: Wageningen UR, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands, rene.vandervlugt@wur.nl.
authors.forename: René A A
authors.initials: RA
authors.lastname: van der Vlugt
authors.forename: Henry
authors.initials: H
authors.lastname: van Raaij
authors.forename: Marjanne
authors.initials: M
authors.lastname: de Weerdt
authors.forename: Jan H W
authors.initials: JH
authors.lastname: Bergervoet
mesh_terms: D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D009693:Nucleic Acid Hybridization; D010935:Plant Diseases / Q000821:virology; D018515:Plant Leaves / Q000821:virology*; D010942:Plant Viruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000032:analysis* / Q000235:genetics
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral