title: Proteomic analysis of nitrate-dependent acetone degradation by Alicycliphilus denitrificans strain BC.
abstract: Alicycliphilus denitrificans strain BC grows anaerobically on acetone with nitrate as electron acceptor. Comparative proteomics of cultures of A. denitrificans strain BC grown on either acetone or acetate with nitrate was performed to study the enzymes involved in the acetone degradation pathway. In the proposed acetone degradation pathway, an acetone carboxylase converts acetone to acetoacetate, an AMP-dependent synthetase/ligase converts acetoacetate to acetoacetyl-CoA, and an acetyl-CoA acetyltransferase cleaves acetoacetyl-CoA to two acetyl-CoA. We also found a putative aldehyde dehydrogenase associated with acetone degradation. This enzyme functioned as a β-hydroxybutyrate dehydrogenase catalyzing the conversion of surplus acetoacetate to β-hydroxybutyrate that may be converted to the energy and carbon storage compound, poly-β-hydroxybutyrate. Accordingly, we confirmed the formation of poly-β-hydroxybutyrate in acetone-grown cells of strain BC. Our findings provide insight in nitrate-dependent acetone degradation that is activated by carboxylation of acetone. This will aid studies of similar pathways found in other microorganisms degrading acetone with nitrate or sulfate as electron acceptor.
authors.affiliation: Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, the Netherlands.
authors.forename: Margreet J
authors.initials: MJ
authors.lastname: Oosterkamp
authors.affiliation: Laboratory of Biochemistry, Wageningen University, Dreijenplein 3, 6703 HA Wageningen, the Netherlands.
authors.forename: Sjef
authors.initials: S
authors.lastname: Boeren
authors.affiliation: Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, the Netherlands.
authors.forename: Siavash
authors.initials: S
authors.lastname: Atashgahi
authors.affiliation: Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, the Netherlands.
authors.forename: Caroline M
authors.initials: CM
authors.lastname: Plugge
authors.affiliation: Laboratory of Systems and Synthetic Biology, Wageningen University, Dreijenplein 10, 6703 HB, Wageningen, the Netherlands.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Schaap
authors.affiliation: Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, the Netherlands Centre of Biological Engineering, Campus de Gualtar, University of Minho, 4710-057, Braga, Portugal fons.stams@wur.nl.
authors.forename: Alfons J M
authors.initials: AJ
authors.lastname: Stams
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000378:metabolism; D000090:Acetoacetates / Q000378:metabolism; D000096:Acetone / Q000378:metabolism*; D000105:Acetyl Coenzyme A / Q000378:metabolism; D000214:Acyl Coenzyme A / Q000378:metabolism; D000444:Aldehyde Dehydrogenase / Q000302:isolation & purification / Q000378:metabolism; D002262:Carboxy-Lyases / Q000378:metabolism; D042621:Comamonadaceae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D009566:Nitrates / Q000378:metabolism*; D010802:Phylogeny; D011091:Polyesters / Q000378:metabolism; D040901:Proteomics; D000094122:Polyhydroxybutyrates
chemical_list: D020155:3-Hydroxybutyric Acid; D000090:Acetoacetates; D000096:Acetone; D000105:Acetyl Coenzyme A; D000214:Acyl Coenzyme A; D000444:Aldehyde Dehydrogenase; D002262:Carboxy-Lyases; D006885:Hydroxybutyrates; D009566:Nitrates; D011091:Polyesters; C010667:acetoacetyl CoA; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates; C016635:acetoacetic acid; C100963:acetone carboxylase
keywords: Alicycliphilus denitrificans; acetone; anaerobic; nitrate; proteome; β-hydroxybutyrate