title: LC-MS-based serum metabolomic analysis reveals dysregulation of phosphatidylcholines in esophageal squamous cell carcinoma.
abstract: Esophageal squamous cell carcinoma (ESCC) is one of the most aggressive cancers with poor prognosis. Here, we carried out liquid chromatography-quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS)-based untargeted metabolomic analysis of ESCC serum samples. Statistical analysis resulted in the identification of 652 significantly dysregulated molecular features in serum from ESCC patients as compared to the healthy subjects. Phosphatidylcholines were identified as a major class of dysregulated metabolites in this study suggesting potential perturbation of phosphocholine metabolism in ESCC. By using a targeted MS/MS approach both in positive and negative mode, we were able to characterize and confirm the structure of seven metabolites. Our study describes a quantitative LC-MS approach for characterizing dysregulated lipid metabolism in ESCC.
  
  
  CONCLUSIONS
  Altered metabolism is a hallmark of cancer. We carried out (LC-MS)-based untargeted metabolomic profiling of serum from esophageal squamous cell carcinoma (ESCC) patients to characterize dysregulated metabolites. Phosphatidylcholine metabolism was found to be significantly altered in ESCC. Our study illustrates the use of mass spectrometry-based metabolomic analysis to characterize molecular alterations associated with ESCC. This article is part of a Special Issue entitled: Proteomics in India.
authors.affiliation: Institute of Bioinformatics, International Technology Park, Bangalore 560066, India; Manipal University, Manipal 576104, India.
authors.forename: Sartaj Ahmad
authors.initials: SA
authors.lastname: Mir
authors.affiliation: Institute of Bioinformatics, International Technology Park, Bangalore 560066, India; School of Biotechnology, KIIT University, Bhubaneswar 751024, India.
authors.forename: Pavithra
authors.initials: P
authors.lastname: Rajagopalan
authors.affiliation: Institute of Bioinformatics, International Technology Park, Bangalore 560066, India; School of Biotechnology, KIIT University, Bhubaneswar 751024, India.
authors.forename: Ankit P
authors.initials: AP
authors.lastname: Jain
authors.affiliation: Institute of Bioinformatics, International Technology Park, Bangalore 560066, India; School of Biotechnology, KIIT University, Bhubaneswar 751024, India.
authors.forename: Aafaque Ahmad
authors.initials: AA
authors.lastname: Khan
authors.affiliation: Institute of Bioinformatics, International Technology Park, Bangalore 560066, India; School of Biotechnology, KIIT University, Bhubaneswar 751024, India.
authors.forename: Keshava K
authors.initials: KK
authors.lastname: Datta
authors.affiliation: Institute of Bioinformatics, International Technology Park, Bangalore 560066, India.
authors.forename: Sonali V
authors.initials: SV
authors.lastname: Mohan
authors.affiliation: Agilent Technologies, Bangalore 560048, India.
authors.forename: Syed Salman
authors.initials: SS
authors.lastname: Lateef
authors.affiliation: Institute of Bioinformatics, International Technology Park, Bangalore 560066, India.
authors.forename: Nandini
authors.initials: N
authors.lastname: Sahasrabuddhe
authors.affiliation: Institute of Bioinformatics, International Technology Park, Bangalore 560066, India.
authors.forename: B L
authors.initials: BL
authors.lastname: Somani
authors.affiliation: Institute of Bioinformatics, International Technology Park, Bangalore 560066, India.
authors.forename: T S
authors.initials: TS
authors.lastname: Keshava Prasad
authors.affiliation: Institute of Bioinformatics, International Technology Park, Bangalore 560066, India.
authors.forename: Aditi
authors.initials: A
authors.lastname: Chatterjee
authors.affiliation: Department of Surgery, Kidwai Memorial Institute of Oncology, Bangalore 560029, India.
authors.forename: K V
authors.initials: KV
authors.lastname: Veerendra Kumar
authors.affiliation: Department of Surgery, Kidwai Memorial Institute of Oncology, Bangalore 560029, India.
authors.forename: M
authors.initials: M
authors.lastname: VijayaKumar
authors.affiliation: Department of Pathology, Kidwai Memorial Institute of Oncology, Bangalore 560029, India.
authors.forename: Rekha V
authors.initials: RV
authors.lastname: Kumar
authors.affiliation: Agilent Technologies, Bangalore 560048, India.
authors.forename: Seetaramanjaneyulu
authors.initials: S
authors.lastname: Gundimeda
authors.affiliation: McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
authors.forename: Akhilesh
authors.initials: A
authors.lastname: Pandey
authors.affiliation: Institute of Bioinformatics, International Technology Park, Bangalore 560066, India. Electronic address: harsha@ibioinformatics.org.
authors.forename: Harsha
authors.initials: H
authors.lastname: Gowda
mesh_terms: D000328:Adult; D002294:Carcinoma, Squamous Cell / Q000097:blood*; D004938:Esophageal Neoplasms / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D055432:Metabolomics; D008875:Middle Aged; D010713:Phosphatidylcholines / Q000097:blood*
chemical_list: D010713:Phosphatidylcholines
keywords: Cancer; Cholines; Lyso PCs; Metabolism; PCs; Serum metabolome