title: IgM antibodies towards pre-endothelial cells: strong indication for an association with accelerated rejection. A case report.
abstract: A 27-year-old woman developed a graft loss due to an accelerated humoral rejection after receiving a blood group identical, human leucocyte antigens (HLA) haploidentical living-related kidney, despite the fact that she did not refer any sensitization event before transplantation. The complement-dependent cytotoxicity and flow cytometry crossmatches were negative for T and B cells. Retrospectively, IgM antibodies against donor precursor endothelial Tie-2(+) cells were detected using a commercially available assay and the pre-transplant serum sample. This case illustrates the necessity of detection of other than the classical HLA-directed antibodies prior organ grafting.
authors.affiliation: Immunology Laboratory and National Tissue Typing Center, General Hospital of Athens "G.Gennimatas", Greece.
authors.forename: Maria L
authors.initials: ML
authors.lastname: Kafetzi
authors.affiliation: Immunology Laboratory and National Tissue Typing Center, General Hospital of Athens "G.Gennimatas", Greece.
authors.forename: Aliki G
authors.initials: AG
authors.lastname: Iniotaki
authors.affiliation: Department of Pathology, Laikon University Hospital of Athens, Greece.
authors.forename: George C
authors.initials: GC
authors.lastname: Liapis
authors.affiliation: Department of Nephrology and Transplantation Centre, Laikon University Hospital of Athens, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: N Darema
authors.affiliation: Department of Immunohaematology and Blood Transfusion, Leiden University Medical Centre, Leiden, the Netherlands.
authors.forename: Ilias I N
authors.initials: II
authors.lastname: Doxiadis
authors.affiliation: Department of Nephrology and Transplantation Centre, Laikon University Hospital of Athens, Greece.
authors.forename: John N
authors.initials: JN
authors.lastname: Boletis
keywords: Tie-2 antibodies; accelerated rejection