title: Human olfactory receptor responses to odorants.
abstract: Although the human olfactory system is capable of discriminating a vast number of odors, we do not currently understand what chemical features are encoded by olfactory receptors. In large part this is due to a paucity of data in a search space covering the interactions of hundreds of receptors with billions of odorous molecules. Of the approximately 400 intact human odorant receptors, only 10% have a published ligand. Here we used a heterologous luciferase assay to screen 73 odorants against a clone library of 511 human olfactory receptors. This dataset will allow other researchers to interrogate the combinatorial nature of olfactory coding.
authors.affiliation: Monell Chemical Senses Center , 3500 Market Street, Philadelphia, Pennsylvania 19104, USA ; Department of Molecular Genetics and Microbiology, Duke University Medical Center , Research Drive, Durham, North Carolina 27710, USA ; Department of Neuroscience, University of Pennsylvania School of Medicine , Philadelphia, Pennsylvania 19104, USA.
authors.forename: Joel D
authors.identifier: 0000-0002-5056-4598
authors.initials: JD
authors.lastname: Mainland
authors.affiliation: Department of Molecular Genetics and Microbiology, Duke University Medical Center , Research Drive, Durham, North Carolina 27710, USA.
authors.forename: Yun R
authors.identifier: 0000-0002-8077-4975
authors.initials: YR
authors.lastname: Li
authors.affiliation: Department of Molecular Genetics and Microbiology, Duke University Medical Center , Research Drive, Durham, North Carolina 27710, USA.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhou
authors.affiliation: Department of Molecular Genetics and Microbiology, Duke University Medical Center , Research Drive, Durham, North Carolina 27710, USA.
authors.forename: Wen Ling L
authors.initials: WL
authors.lastname: Liu
authors.affiliation: Department of Molecular Genetics and Microbiology, Duke University Medical Center , Research Drive, Durham, North Carolina 27710, USA ; Department of Neurobiology and Duke Institute for Brain Sciences, Duke University Medical Center , Research Drive, Durham, North Carolina 27710, USA.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Matsunami
mesh_terms: D001681:Biological Assay; D006801:Humans; D008024:Ligands; D008156:Luciferases; D009812:Odorants; D018035:Receptors, Odorant / Q000378:metabolism
chemical_list: D008024:Ligands; D018035:Receptors, Odorant; D008156:Luciferases