title: Genome-wide screening of salt tolerant genes by activation-tagging using dedifferentiated calli of Arabidopsis and its application to finding gene for Myo-inositol-1-p-synthase.
abstract: Salinity represents a major abiotic stress factor that can adversely limit the production, quality and geographical distribution of crops. In this study we focused on dedifferentiated calli with fundamental cell functions, the salt tolerance of which had not been previously examined. The experimental approach was based on activation tagging without regeneration of plants for the identification of salt-tolerant mutants of Arabidopsis. Among 62,000 transformed calli that were screened, 18 potential mutants resistant to 150 mM NaCl were obtained. Thermal asymmetric interlaced (TAIL)-PCR was performed to determine the location of T-DNA integration in the genome. In one line, referred to as salt tolerant callus 1 (stc1), expression of a gene [At4g39800: myo-inositol-1-P-synthase 1 (MIPS1)] was considerably enhanced in calli. Plants regenerated from calli showed tolerance to salt in germination and subsequent growth. Retransformation of wild-type Arabidopsis with MIPS1 conferred salt tolerance, indicating that MIPS1 is the causal gene. The over-expression of MIPS1 increased the content of total inositol. The involvement of MIPS1 in salt tolerance through the fundamental cell growth has been proved in Arabidopsis.
authors.affiliation: Laboratory of Plant Molecular Improvement, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, 52-1 Yada, Suruga, Shizuoka 422-8526, Japan.
authors.forename: Aftab
authors.initials: A
authors.lastname: Ahmad
authors.affiliation: Laboratory of Plant Molecular Improvement, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, 52-1 Yada, Suruga, Shizuoka 422-8526, Japan.
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Niwa
authors.affiliation: Laboratory of Plant Molecular Improvement, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, 52-1 Yada, Suruga, Shizuoka 422-8526, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Goto
authors.affiliation: Laboratory of Plant Molecular Improvement, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, 52-1 Yada, Suruga, Shizuoka 422-8526, Japan.
authors.forename: Kyoko
authors.initials: K
authors.lastname: Kobayashi
authors.affiliation: Laboratory of Plant Molecular Improvement, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, 52-1 Yada, Suruga, Shizuoka 422-8526, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Shimizu
authors.affiliation: Laboratory of Protein Engineering, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, 52-1 Yada, Suruga, Shizuoka 422-8526, Japan.
authors.forename: Sohei
authors.initials: S
authors.lastname: Ito
authors.affiliation: Laboratory of Molecular Fooineering, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, 52-1 Yada, Suruga, Shizuoka 422-8526, Japan.
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Usui
authors.affiliation: Laboratory of Molecular Fooineering, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, 52-1 Yada, Suruga, Shizuoka 422-8526, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Nakayama
authors.affiliation: Laboratory of Plant Molecular Improvement, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, 52-1 Yada, Suruga, Shizuoka 422-8526, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Kobayashi
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics; D007296:Myo-Inositol-1-Phosphate Synthase / Q000235:genetics / Q000378:metabolism*; D055049:Salt Tolerance; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D029681:Arabidopsis Proteins; D012965:Sodium Chloride; D007296:Myo-Inositol-1-Phosphate Synthase