title: ATG12-ATG3 connects basal autophagy and late endosome function.
abstract: In addition to supporting cell survival in response to starvation or stress, autophagy promotes basal protein and organelle turnover. Compared to our understanding of stress-induced autophagy, little is known about how basal autophagy is regulated and how its activity is coordinated with other cellular processes. We recently identified a novel interaction between the ATG12-ATG3 conjugate and the ESCRT-associated protein PDCD6IP/Alix that promotes basal autophagy and endolysosomal trafficking. Moreover, ATG12-ATG3 is required for diverse PDCD6IP-mediated functions including late endosome distribution, exosome secretion, and viral budding. Our results highlight the importance of late endosomes for basal autophagic flux and reveal distinct roles for the core autophagy proteins ATG12 and ATG3 in controlling late endosome function.
authors.affiliation: a Department of Pathology and Helen Diller Family Comprehensive Cancer Center; University of California ; San Francisco , CA USA.
authors.forename: Lyndsay
authors.initials: L
authors.lastname: Murrow
authors.forename: Jayanta
authors.initials: J
authors.lastname: Debnath
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001343:Autophagy / Q000502:physiology*; D011992:Endosomes / Q000378:metabolism*; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D025841:Small Ubiquitin-Related Modifier Proteins / Q000378:metabolism*; D044763:Ubiquitin-Conjugating Enzymes / Q000378:metabolism*
chemical_list: D008869:Microtubule-Associated Proteins; D025841:Small Ubiquitin-Related Modifier Proteins; D044763:Ubiquitin-Conjugating Enzymes
keywords: ATG12; ATG3; PDCD6IP/Alix; basal autophagy; exosome; late endosome; viral budding