title: DNA nanostructures based biosensor for the determination of aromatic compounds.
abstract: Graphite electrode was modified using multi-walled carbon nanotubes (MWCNT), chitosan (CS), glutaraldehyde (GTA) and DNA nanostructures (nsDNA). DNA nanostructures of 50 nm in size were produced from single DNA template sequence using a simple two step procedure and were confirmed using TEM and AFM analysis. The modified electrode was applied to the electrochemical detection of aromatic compounds using EIS. The modified electrode was characterized using differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). For comparison, electrochemical results derived from single stranded (50 bp length) and double stranded (50 bp length) DNA based biosensors were used. The results indicate that the modified electrode prior to nsDNA immobilization provides a viable platform that effectively promotes electron transfer between nsDNA and the electrode. The mode of binding between the nsDNA and aromatic compounds was investigated using EIS, indicating that the dominant interaction is non-covalent. nsDNA based biosensor was observed to act as an efficient biosensor in selective and sensitive identification of aromatic compounds.
authors.affiliation: Department of Chemistry, SRM University, Kattankulathur 603203, India.
authors.forename: S Baby
authors.initials: SB
authors.lastname: Gayathri
authors.affiliation: Department of Chemistry, SRM University, Kattankulathur 603203, India.
authors.forename: P
authors.initials: P
authors.lastname: Kamaraj
authors.affiliation: Department of Chemistry, SRM University, Kattankulathur 603203, India.
authors.forename: M
authors.initials: M
authors.lastname: Arthanareeswari
authors.affiliation: Department of Chemistry, SRM University, Kattankulathur 603203, India.
authors.forename: S
authors.initials: S
authors.lastname: Devikala
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D048271:Chitosan / Q000737:chemistry; D058266:Dielectric Spectroscopy; D004566:Electrodes; D006108:Graphite / Q000737:chemistry; D006841:Hydrocarbons, Aromatic / Q000032:analysis*; D061925:Immobilized Nucleic Acids / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D037742:Nanotubes, Carbon / Q000737:chemistry
chemical_list: D006841:Hydrocarbons, Aromatic; D061925:Immobilized Nucleic Acids; D037742:Nanotubes, Carbon; D006108:Graphite; D048271:Chitosan
keywords: Aromatic compounds; Biosensor; DNA nanostructures; Electrochemical characterization; Electrochemical impedance spectroscopy; Multi-walled carbon nanotubes