title: Borna disease virus infection impacts microRNAs associated with nervous system development, cell differentiation, proliferation and apoptosis in the hippocampi of neonatal rats.
abstract: MicroRNAs (miRNAs) regulate gene expression by inhibiting transcription or translation and are involved in diverse biological processes, including development, cellular differentiation and tumor generation. miRNA microarray technology is a high‑throughput global analysis tool for miRNA expression profiling. Here, the hippocampi of four borna disease virus (BDV)‑infected and four non‑infected control neonatal rats were selected for miRNA microarray and bioinformatic analysis. Reverse transcription quantitative polymerase chain reaction (RT‑qPCR) analysis was subsequently performed to validate the dysregulated miRNAs. Seven miRNAs (miR‑145*, miR‑146a*, miR‑192*, miR‑200b, miR‑223*, miR‑449a and miR‑505), showed increased expression, whereas two miRNAs (miR‑126 and miR‑374) showed decreased expression in the BDV‑infected group. By RT‑qPCR validation, five miRNAs (miR‑126, miR‑200b, miR‑374, miR‑449a and miR‑505) showed significantly decreased expression (P<0.05) in response to BDV infection. Biocarta pathway analysis predicted target genes associated with 'RNA', 'IGF1mTOR', 'EIF2', 'VEGF', 'EIF', 'NTHI', 'extrinsic', 'RB', 'IL1R' and 'IGF1' pathways. Gene Ontology analysis predicted target genes associated with 'peripheral nervous system development', 'regulation of small GTPase-mediated signal transduction', 'regulation of Ras protein signal transduction', 'aerobic respiration', 'membrane fusion', 'positive regulation of cell cycle', 'cellular respiration', 'heterocycle metabolic process', 'protein tetramerization' and 'regulation of Rho protein signal transduction' processes. Among the five dysregulated miRNAs identified by RT‑qPCR, miR‑126, miR‑200b and miR‑449a showed a strong association with nervous system development, cell differentiation, proliferation and apoptosis.
authors.affiliation: Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402460, P.R. China.
authors.forename: Mingjun
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Institute of Neuroscience and the Collaborative Innovation Centre for Brain Science, Chongqing Medical University, Chongqing 400016, P.R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Sun
authors.affiliation: Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402460, P.R. China.
authors.forename: Shigang
authors.initials: S
authors.lastname: Chen
authors.affiliation: Institute of Neuroscience and the Collaborative Innovation Centre for Brain Science, Chongqing Medical University, Chongqing 400016, P.R. China.
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.affiliation: Institute of Neuroscience and the Collaborative Innovation Centre for Brain Science, Chongqing Medical University, Chongqing 400016, P.R. China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402460, P.R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: He
authors.affiliation: Institute of Neuroscience and the Collaborative Innovation Centre for Brain Science, Chongqing Medical University, Chongqing 400016, P.R. China.
authors.forename: Xia
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402460, P.R. China.
authors.forename: Lujun
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402460, P.R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402460, P.R. China.
authors.forename: Deyu
authors.initials: D
authors.lastname: Yang
authors.affiliation: Institute of Neuroscience and the Collaborative Innovation Centre for Brain Science, Chongqing Medical University, Chongqing 400016, P.R. China.
authors.forename: Rongzhong
authors.initials: R
authors.lastname: Huang
authors.affiliation: Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402460, P.R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: Xie
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000235:genetics / Q000254:growth & development / Q000821:virology*; D017209:Apoptosis; D001891:Borna disease virus / Q000235:genetics*; D028981:Bornaviridae / Q000302:isolation & purification*; D002454:Cell Differentiation; D049109:Cell Proliferation; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006624:Hippocampus / Q000254:growth & development / Q000378:metabolism / Q000821:virology*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D017207:Rats, Sprague-Dawley / Q000235:genetics / Q000254:growth & development / Q000821:virology*
chemical_list: D035683:MicroRNAs