title: Increased SUMO-1 expression in response to hypoxia: Interaction with HIF-1α in hypoxic pulmonary hypertension.
abstract: Pulmonary hypertension (PH) develops in 30-70% of chronic obstructive pulmonary disease patients and increases morbidity and mortality. The present study aimed to investigate the regulation of small ubiquitin‑related modifier‑1 (SUMO‑1) expression in response to hypoxia. The experiments were carried out in vitro in rat pulmonary arterial smooth muscle cells (PASMCs) and in vivo using a rat hypoxic PH (HPH) model. A significant increase in SUMO‑1 mRNA and protein levels was observed following hypoxic stimulation in vivo and in vitro. SUMO‑1 is known to interact with various transcription factors, including hypoxia‑inducible factor‑1α (HIF‑1α) in vitro. Notably, the expression of HIF‑1α and its target gene, vascular endothelial growth factor, was increased by hypoxia in HPH. In addition, the present data suggest that SUMO‑1 regulated HIF‑1α in response to hypoxia (gene silencing and overexpression). Finally, the co‑immunoprecipitation assays suggest a direct and specific interaction between SUMO‑1 and HIF‑1α. In conclusion, SUMO‑1 may participate in the modulation of HIF‑1α through sumoylation in HPH. However, further studies are required to confirm this.
authors.affiliation: Institute of Respiratory Medicine, Hunan Province Geriatric Hospital, Changsha, Hunan 410016, P.R. China.
authors.forename: Yongliang
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Institute of Respiratory Medicine, Hunan Province Geriatric Hospital, Changsha, Hunan 410016, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Institute of Respiratory Medicine, Hunan Province Geriatric Hospital, Changsha, Hunan 410016, P.R. China.
authors.forename: Hua
authors.initials: H
authors.lastname: Tian
authors.affiliation: Institute of Respiratory Medicine, Hunan Province Geriatric Hospital, Changsha, Hunan 410016, P.R. China.
authors.forename: Guang
authors.initials: G
authors.lastname: Li
authors.affiliation: Institute of Respiratory Medicine, Hunan Province Geriatric Hospital, Changsha, Hunan 410016, P.R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Institute of Respiratory Medicine, Hunan Province Geriatric Hospital, Changsha, Hunan 410016, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.affiliation: Institute of Respiratory Medicine, Hunan Province Geriatric Hospital, Changsha, Hunan 410016, P.R. China.
authors.forename: Ruicheng
authors.initials: R
authors.lastname: Hu
authors.affiliation: Institute of Respiratory Medicine, Hunan Province Geriatric Hospital, Changsha, Hunan 410016, P.R. China.
authors.forename: Aiguo
authors.initials: A
authors.lastname: Dai
mesh_terms: D000818:Animals; D015687:Cell Hypoxia / Q000502:physiology*; D002478:Cells, Cultured; D006976:Hypertension, Pulmonary / Q000503:physiopathology*; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000096:biosynthesis*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D011651:Pulmonary Artery / Q000473:pathology*; D029424:Pulmonary Disease, Chronic Obstructive / Q000150:complications; D012333:RNA, Messenger / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D025842:SUMO-1 Protein / Q000096:biosynthesis* / Q000235:genetics; D058207:Sumoylation; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis
chemical_list: C497444:Hif1a protein, rat; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D012333:RNA, Messenger; D025842:SUMO-1 Protein; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat