title: Molecular Cloning and Functional Characterization of Mannose Receptor in Zebra Fish (Danio rerio) during Infection with Aeromonas sobria.
abstract: Mannose receptor (MR) is a member of pattern-recognition receptors (PRRs), which plays a significant role in immunity responses. Much work on MR has been done in mammals and birds while little in fish. In this report, a MR gene (designated as zfMR) was cloned from zebra fish (Danio rerio), which is an attractive model for the studies of animal diseases. The full-length cDNA of zfMR contains 6248 bp encoding a putative protein of 1428 amino acids. The predicted amino acid sequences showed that zfMR contained a cysteine-rich domain, a single fibronectin type II (FN II) domain, eight C-type lectin-like domains (CTLDs), a transmembrane domain and a short C-terminal cytoplasmic domain, sharing highly conserved structures with MRs from the other species. The MR mRNA could be detected in all examined tissues with highest level in kidney. The temporal expression patterns of MR, IL-1β and TNF-α mRNAs were analyzed in the liver, spleen, kidney and intestine post of infection with Aeromonas sobria. By immunohistochemistry assay, slight enhancement of MR protein was also observed in the spleen and intestine of the infected zebra fish. The established zebra fish-A. sobria infection model will be valuable for elucidating the role of MR in fish immune responses to infection.
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China. zhengfeifei@webmail.hzau.edu.cn.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China. zhengfeifei@webmail.hzau.edu.cn.
authors.forename: Feifei
authors.initials: F
authors.lastname: Zheng
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China. asim_m97@yahoo.com.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China. asim_m97@yahoo.com.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Asim
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China. lanjiangfeng@mail.hzau.edu.cn.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China. lanjiangfeng@mail.hzau.edu.cn.
authors.forename: Jiangfeng
authors.initials: J
authors.lastname: Lan
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China. zhaolijuan4234@163.com.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China. weishun@mail.hzau.edu.cn.
authors.forename: Shun
authors.initials: S
authors.lastname: Wei
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China. chennan@mail.hzau.edu.cn.
authors.forename: Nan
authors.initials: N
authors.lastname: Chen
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China. liuxl@mail.hzau.edu.cn.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China. liuxl@mail.hzau.edu.cn.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China. zhouyang@mail.hzau.edu.cn.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China. zhouyang@mail.hzau.edu.cn.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China. linli@mail.hzau.edu.cn.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China. linli@mail.hzau.edu.cn.|Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan 430070, China. linli@mail.hzau.edu.cn.|Agricultural Bioinformatics Key Laboratory of Hubei Province, College of Informatics, Huazhong Agricultural University, Wuhan 430070, China. linli@mail.hzau.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Lin
mesh_terms: D016980:Aeromonas hydrophila / Q000502:physiology*; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D016905:Gram-Negative Bacterial Infections / Q000378:metabolism / Q000473:pathology / Q000662:veterinary; D006801:Humans; D007150:Immunohistochemistry; D053583:Interleukin-1beta / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D037181:Lectins, C-Type / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D011956:Receptors, Cell Surface / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D013154:Spleen / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D015027:Zebrafish / Q000378:metabolism* / Q000382:microbiology
chemical_list: D053583:Interleukin-1beta; D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D014409:Tumor Necrosis Factor-alpha
keywords: Aeromonas sobria; Danio rerio; mRNA expression; mannose receptor; protein expression