title: Metabolic differences in ripening of Solanum lycopersicum 'Ailsa Craig' and three monogenic mutants.
abstract: Application of mass spectrometry enables the detection of metabolic differences between groups of related organisms. Differences in the metabolic fingerprints of wild-type Solanum lycopersicum and three monogenic mutants, ripening inhibitor (rin), non-ripening (nor) and Colourless non-ripening (Cnr), of tomato are captured with regard to ripening behaviour. A high-resolution tandem mass spectrometry system coupled to liquid chromatography produced a time series of the ripening behaviour at discrete intervals with a focus on changes post-anthesis. Internal standards and quality controls were used to ensure system stability. The raw data of the samples and reference compounds including study protocols have been deposited in the open metabolomics database MetaboLights via the metadata annotation tool Isatab to enable efficient re-use of the datasets, such as in metabolomics cross-study comparisons or data fusion exercises.
authors.affiliation: European Molecular Biology Laboratory-European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus , Hinxton, Cambridge CB10 2HA, UK.
authors.forename: Stephan
authors.identifier: 0000-0002-2412-4118
authors.initials: S
authors.lastname: Beisken
authors.affiliation: Syngenta Jealott's Hill International Research Centre , Bracknell, Berkshire RG42 6EY, UK.
authors.forename: Mark
authors.initials: M
authors.lastname: Earll
authors.affiliation: Syngenta Jealott's Hill International Research Centre , Bracknell, Berkshire RG42 6EY, UK.
authors.forename: Charles
authors.initials: C
authors.lastname: Baxter
authors.affiliation: Syngenta Jealott's Hill International Research Centre , Bracknell, Berkshire RG42 6EY, UK.
authors.forename: David
authors.initials: D
authors.lastname: Portwood
authors.affiliation: Syngenta Jealott's Hill International Research Centre , Bracknell, Berkshire RG42 6EY, UK.
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Ament
authors.affiliation: Syngenta Jealott's Hill International Research Centre , Bracknell, Berkshire RG42 6EY, UK.
authors.forename: Aniko
authors.initials: A
authors.lastname: Kende
authors.affiliation: Centre for Plant Integrative Biology, University of Nottingham , Loughborough, Leicestershire LE12 5RD, UK.
authors.forename: Charlie
authors.initials: C
authors.lastname: Hodgman
authors.affiliation: Centre for Plant Integrative Biology, University of Nottingham , Loughborough, Leicestershire LE12 5RD, UK.
authors.forename: Graham
authors.initials: G
authors.lastname: Seymour
authors.affiliation: Centre for Plant Integrative Biology, University of Nottingham , Loughborough, Leicestershire LE12 5RD, UK.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Smith
authors.affiliation: School of Biological Sciences, Royal Holloway, University of London, Egham Hill , Egham, Surrey TW20 0EX, UK.
authors.forename: Paul
authors.initials: P
authors.lastname: Fraser
authors.affiliation: Syngenta Jealott's Hill International Research Centre , Bracknell, Berkshire RG42 6EY, UK.
authors.forename: Mark
authors.initials: M
authors.lastname: Seymour
authors.affiliation: European Molecular Biology Laboratory-European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus , Hinxton, Cambridge CB10 2HA, UK.
authors.forename: Reza M
authors.identifier: 0000-0001-8604-1732
authors.initials: RM
authors.lastname: Salek
authors.affiliation: European Molecular Biology Laboratory-European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus , Hinxton, Cambridge CB10 2HA, UK.
authors.forename: Christoph
authors.initials: C
authors.lastname: Steinbeck
mesh_terms: D016208:Databases, Factual; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D018551:Solanum lycopersicum / Q000235:genetics / Q000378:metabolism*; D055432:Metabolomics; D009154:Mutation