title: Lamellar sheet exfoliation of single lipid vesicles by a membrane-active peptide.
abstract: Using total internal fluorescence microscopy, highly parallel measurements of single lipid vesicles unexpectedly reveal that a small fraction of vesicles rupture in multiple discrete steps when destabilized by a membrane-active peptide which is in contrast to classical solubilization models. To account for this surprizing kinetic behaviour, we identified that this vesicle subpopulation consists of multilamellar vesicles, and that the outermost lamella is more susceptible to rupture than unilamellar vesicles of even smaller size. This finding sheds light on the multiple ways in which membrane configuration can influence strain in the bilayer leaflet and contribute to nm-scale membrane curvature sensing.
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue 639798, Singapore. njcho@ntu.edu.sg.
authors.forename: Seyed R
authors.initials: SR
authors.lastname: Tabaei
authors.forename: N J
authors.initials: NJ
authors.lastname: Cho
mesh_terms: D000595:Amino Acid Sequence; D019921:Biotinylation; D000067493:Dynamic Light Scattering; D005452:Fluoresceins / Q000737:chemistry; D007700:Kinetics; D008081:Liposomes / Q000138:chemical synthesis / Q000737:chemistry* / Q000648:ultrastructure*; D008856:Microscopy, Fluorescence; D008956:Models, Chemical; D049329:Nanostructures; D010455:Peptides / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000737:chemistry; D010714:Phosphatidylethanolamines / Q000737:chemistry; D011485:Protein Binding; D012235:Rhodamines / Q000737:chemistry; D012995:Solubility
chemical_list: D005452:Fluoresceins; D008081:Liposomes; C053685:N-(lissamine rhodamine B sulfonyl)-1,2-dioleoylphosphatidylethanolamine; D010455:Peptides; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D012235:Rhodamines; C028694:1-palmitoyl-2-oleoylphosphatidylcholine; C007740:fluorexon