title: Formaldehyde cross-linking and structural proteomics: Bridging the gap.
abstract: Proteins are dynamic entities constantly moving and altering their structures based on their functions and interactions inside and outside the cell. Formaldehyde cross-linking combined with mass spectrometry can accurately capture interactions of these rapidly changing biomolecules while maintaining their physiological surroundings. Even with its numerous established uses in biology and compatibility with mass spectrometry, formaldehyde has not yet been applied in structural proteomics. However, formaldehyde cross-linking is moving toward analyzing tertiary structure, which conventional cross-linkers have already accomplished. The purpose of this review is to describe the potential of formaldehyde cross-linking in structural proteomics by highlighting its applications, characteristics and current status in the field.
authors.affiliation: The Biomedical Research Centre, University of British Columbia, Vancouver, BC V6T 1Z3, Canada; Department of Chemistry, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.
authors.forename: Savita
authors.initials: S
authors.lastname: Srinivasa
authors.affiliation: The Biomedical Research Centre, University of British Columbia, Vancouver, BC V6T 1Z3, Canada; Department of Chemistry, University of British Columbia, Vancouver, BC V6T 1Z3, Canada; Institute for Genomics, Biocomputing and Biotechnology, Mississippi State University, Mississippi State, MS 39762, United States.
authors.forename: Xuan
authors.initials: X
authors.lastname: Ding
authors.affiliation: The Biomedical Research Centre, University of British Columbia, Vancouver, BC V6T 1Z3, Canada; Department of Chemistry, University of British Columbia, Vancouver, BC V6T 1Z3, Canada; Centre for Blood Research, University of British Columbia, Vancouver, BC V6T 1Z3, Canada. Electronic address: kast@brc.ubc.ca.
authors.forename: Juergen
authors.initials: J
authors.lastname: Kast
mesh_terms: D000818:Animals; D003432:Cross-Linking Reagents / Q000737:chemistry*; D005557:Formaldehyde / Q000737:chemistry*; D006801:Humans; D007328:Insulin / Q000032:analysis / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D040901:Proteomics / Q000379:methods*
chemical_list: D003432:Cross-Linking Reagents; D007328:Insulin; D005557:Formaldehyde
keywords: Biological applications; Cross-linking; Formaldehyde; Mass spectrometry; Protein interactions; Protein structure