title: Ribonuclease like 5 regulates zebrafish yolk extension by suppressing a p53-dependent DNA damage response pathway.
abstract: Ribonuclease like 5 (Rnasel5) is a novel member of the zebrafish ribonuclease A family and its expression is increased during early embryogenesis. However, the in vivo biological function of Rnasel5 remains to be elucidated. Here, we report that knockdown of Rnasel5 by morhpolinos caused shrunken yolk extension as well as increased DNA damage at yolk syncytial layer and external tissue layers via the activation of p53 pathway. In addition, the morphological defects caused by Rnasel5 knockdown can be partially rescued by mRNA injection. Our findings provide the first functional characterization of Rnasel5 in zebrafish development and reveal its critical role in yolk extension by modulation of the p53 pathway.
authors.affiliation: Institute of Environmental Health, Zhejiang University School of Public Health, Hangzhou 310058, China; Program in Molecular Cell Biology, Zhejiang University School of Medicine, Hangzhou 310058, China; Research Center of Molecular Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China.
authors.forename: Yixing
authors.initials: Y
authors.lastname: Zhai
authors.affiliation: Institute of Environmental Health, Zhejiang University School of Public Health, Hangzhou 310058, China; Program in Molecular Cell Biology, Zhejiang University School of Medicine, Hangzhou 310058, China; Research Center of Molecular Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China.
authors.forename: Xinyuan
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Institute of Environmental Health, Zhejiang University School of Public Health, Hangzhou 310058, China; Program in Molecular Cell Biology, Zhejiang University School of Medicine, Hangzhou 310058, China; Research Center of Molecular Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China.
authors.forename: Jinghao
authors.initials: J
authors.lastname: Sheng
authors.affiliation: Institute of Environmental Health, Zhejiang University School of Public Health, Hangzhou 310058, China; Program in Molecular Cell Biology, Zhejiang University School of Medicine, Hangzhou 310058, China; Research Center of Molecular Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China.
authors.forename: Xiangwei
authors.initials: X
authors.lastname: Gao
authors.affiliation: Key Laboratory for Molecular Animal Nutrition, Ministry of Education, Zhejiang University College of Animal Sciences, Hangzhou 310058, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Ou
authors.affiliation: Institute of Environmental Health, Zhejiang University School of Public Health, Hangzhou 310058, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University, Hangzhou 310003, China; Program in Molecular Cell Biology, Zhejiang University School of Medicine, Hangzhou 310058, China; Research Center of Molecular Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China. Electronic address: zpxu@zju.edu.cn.
authors.forename: Zhengping
authors.initials: Z
authors.lastname: Xu
mesh_terms: D000818:Animals; D004249:DNA Damage; D004625:Embryo, Nonmammalian; D047108:Embryonic Development / Q000502:physiology; D055785:Gene Knockdown Techniques; D012259:Ribonuclease, Pancreatic / Q000235:genetics / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics* / Q000378:metabolism; D015017:Yolk Sac / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D015027:Zebrafish / Q000502:physiology*; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D016159:Tumor Suppressor Protein p53; D029961:Zebrafish Proteins; C571228:tp53 protein, zebrafish; C046366:angiogenin; D012259:Ribonuclease, Pancreatic
keywords: DNA damage; Embryogenesis; P53; Ribonuclease like 5; Yolk extension