title: Designing Two-Dimensional Protein Arrays through Fusion of Multimers and Interface Mutations.
abstract: We have combined fusion of oligomers with cyclic symmetry and alanine substitutions to eliminate clashes and produce proteins that self-assemble into 2-D arrays upon addition of calcium ions. Using TEM, AFM, small-angle X-ray scattering, and fluorescence microscopy, we show that the designed lattices which are 5 nm high with p3 space group symmetry and 7.25 nm periodicity self-assemble into structures that can exceed 100 μm in characteristic length. The versatile strategy, experimental approach, and hexagonal arrays described herein should prove valuable for the engineering of functional nanostructured materials in 2-D.
authors.affiliation: †Department of Chemical Engineering, University of Washington, Seattle, Washington 918195, United States.
authors.forename: James F
authors.initials: JF
authors.lastname: Matthaei
authors.affiliation: ‡Department of Biochemistry, University of Washington, Seattle, Washington 918195, United States.
authors.forename: Frank
authors.initials: F
authors.lastname: DiMaio
authors.affiliation: †Department of Chemical Engineering, University of Washington, Seattle, Washington 918195, United States.
authors.forename: Jeffrey J
authors.initials: JJ
authors.lastname: Richards
authors.affiliation: †Department of Chemical Engineering, University of Washington, Seattle, Washington 918195, United States.
authors.forename: Lilo D
authors.initials: LD
authors.lastname: Pozzo
authors.affiliation: ‡Department of Biochemistry, University of Washington, Seattle, Washington 918195, United States.|§Howard Hughes Medical Institute, 4000 Jones Bridge Road, Chevy Chase, Maryland 20815, United States.
authors.forename: David
authors.initials: D
authors.lastname: Baker
authors.affiliation: †Department of Chemical Engineering, University of Washington, Seattle, Washington 918195, United States.
authors.forename: François
authors.initials: F
authors.lastname: Baneyx
mesh_terms: D019943:Amino Acid Substitution; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics*; D002118:Calcium / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D008958:Models, Molecular; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure*; D040081:Protein Array Analysis / Q000295:instrumentation; D012486:Salmonella typhimurium / Q000737:chemistry / Q000235:genetics*; D014961:X-Ray Diffraction
chemical_list: D001426:Bacterial Proteins; D002118:Calcium
keywords: 2-D materials; Protein design; artificial S-layers; bionanotechnology; self-assembly