title: Validation of a confirmatory method of salbutamol in sheep hair by UPLC-MS/MS and its application to pharmacokinetic study.
abstract: A new method for determining salbutamol in hair of sheep by ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was established. Samples were extracted with 0.1M of HCl solution. The mixture was heated to 60 °C in a water bath and kept at this temperature for 4h. The extracts were purified through SPE method and then dried with nitrogen. Residues were redissolved in mobile phase. The target compound was determined by UPLC-MS/MS with BEH-C18 column. The usefulness and feasibility of different treatment procedures of hair containing salbutamol were evaluated. The range of linearity was 1-100 ng/g. The LOD was 0.3 ng/g, and the LOQ was 1 ng/g. Recoveries were 89-106%, and coefficients of variation were 3.2-13.9%. Pharmacokinetics of salbutamol was studied in healthy sheep after oral administration of 150 μg/kg body weight salbutamol for 21 consecutive days. Salbutamol residues in hair were still detected after 21 days of administration.
authors.affiliation: Institute of Quality Standards and Testing Technology for Agricultural Products, Chinese Academy of Agricultural Science, Beijing 100081 China.
authors.forename: Suo
authors.initials: S
authors.lastname: Decheng
authors.affiliation: Institute of Quality Standards and Testing Technology for Agricultural Products, Chinese Academy of Agricultural Science, Beijing 100081 China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Wei
authors.affiliation: Institute of Quality Standards and Testing Technology for Agricultural Products, Chinese Academy of Agricultural Science, Beijing 100081 China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Institute of Quality Standards and Testing Technology for Agricultural Products, Chinese Academy of Agricultural Science, Beijing 100081 China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Genlong
authors.affiliation: Institute of Quality Standards and Testing Technology for Agricultural Products, Chinese Academy of Agricultural Science, Beijing 100081 China.
authors.forename: Wang
authors.initials: W
authors.lastname: Ruigou
authors.affiliation: Institute of Quality Standards and Testing Technology for Agricultural Products, Chinese Academy of Agricultural Science, Beijing 100081 China.
authors.forename: Wang
authors.initials: W
authors.lastname: PeiLong
authors.affiliation: Institute of Quality Standards and Testing Technology for Agricultural Products, Chinese Academy of Agricultural Science, Beijing 100081 China. Electronic address: suxiaoou@caas.cn.
authors.forename: Su
authors.initials: S
authors.lastname: Xiaoou
mesh_terms: D000284:Administration, Oral; D000420:Albuterol / Q000032:analysis* / Q000493:pharmacokinetics*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006197:Hair / Q000737:chemistry*; D006851:Hydrochloric Acid / Q000737:chemistry; D057230:Limit of Detection; D009584:Nitrogen / Q000737:chemistry; D015203:Reproducibility of Results; D012756:Sheep; D012997:Solvents / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013696:Temperature
chemical_list: D012997:Solvents; D009584:Nitrogen; D000420:Albuterol; D006851:Hydrochloric Acid
keywords: Pharmacokinetic study in sheep; Salbutamol; Sheep hair; UPLC-MS/MS