title: Adjustment of a rapid method for quantification of Fusarium spp. spore suspensions in plant pathology.
abstract: The use of a Neubauer chamber is a broadly employed method when cell suspensions need to be quantified. However, this technique may take a long time and needs trained personnel. Spectrophotometry has proved to be a rapid, simple and accurate method to estimate the concentration of spore suspensions of isolates of the genus Fusarium. In this work we present a linear formula to relate absorbance measurements at 530nm with the number of microconidia/ml in a suspension.
authors.affiliation: INTA EEA La Consulta, La Consulta, Mendoza, Argentina. Electronic address: caligioregei.pablo@inta.gob.ar.
authors.forename: Pablo F
authors.initials: PF
authors.lastname: Caligiore-Gei
authors.affiliation: INTA EEA La Consulta, La Consulta, Mendoza, Argentina.
authors.forename: Jorge G
authors.initials: JG
authors.lastname: Valdez
mesh_terms: D003116:Color; D005670:Fusarium / Q000302:isolation & purification* / Q000502:physiology; D006801:Humans; D016014:Linear Models; D016533:Mycological Typing Techniques; D009172:Mycology / Q000379:methods*; D019697:Onions / Q000382:microbiology; D010935:Plant Diseases / Q000382:microbiology*; D013045:Species Specificity; D013053:Spectrophotometry / Q000379:methods*; D013172:Spores, Fungal / Q000302:isolation & purification*; D013535:Suspensions; D013997:Time Factors
chemical_list: D013535:Suspensions
keywords: Cuantificación de esporas; Espectrofotometría; Laboratory methods; Métodos de laboratorio; Spectrophotometry; Spore counting