title: Identification of jasmonic acid-associated microRNAs and characterization of the regulatory roles of the miR319/TCP4 module under root-knot nematode stress in tomato.
abstract: MicroRNAs (miRNAs) are important transcriptional and post-transcriptional modulators of gene expression that play crucial roles in the responses to diverse stresses. To explore jasmonic acid (JA)-dependent miRNA-mediated regulatory networks that are responsive to root-knot nematode (RKN), two small RNA libraries were constructed from wild-type (WT) and JA mutant (spr2) plants. A total of 263 known miRNAs and 441 novel miRNAs were significantly regulated under RKN stress in the two libraries. The spatio-temporal expression of candidate miRNAs and their corresponding targets were analysed by qRT-PCR under RKN stress. A clear negative correlation was observed between miR319 and its target TEOSINTE BRANCHED1/CYCLOIDEA/PRO-LIFERATING CELL FACTOR 4 (TCP4) in leaf, stem, and root under RKN stress, implying that the miR319/TCP4 module is involved in the systemic defensive response. Reverse genetics demonstrated that the miR319/TCP4 module affected JA synthetic genes and the endogenous JA level in leaves, thereby mediating RKN resistance. These results suggested that the action of miR319 in serving as a systemic signal responder and regulator that modulated the RKN systemic defensive response was mediated via JA. The potential cross-talk between miR319/TCP4 and miR396/GRF (GROWTH RESPONDING FACTOR) in roots under RKN invasion is discussed, and a predictive model regarding miR319/TCP4-mediated RKN resistance is proposed.
authors.affiliation: Beijing Key Laboratory for Agricultural Application and New Technique, Plant Science and Technology College, Beijing University of Agriculture, Beijing, 102206, China.
authors.forename: Wenchao
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Beijing Key Laboratory for Agricultural Application and New Technique, Plant Science and Technology College, Beijing University of Agriculture, Beijing, 102206, China.
authors.forename: Zilong
authors.initials: Z
authors.lastname: Li
authors.affiliation: Beijing Key Laboratory for Agricultural Application and New Technique, Plant Science and Technology College, Beijing University of Agriculture, Beijing, 102206, China.
authors.forename: Jingwei
authors.initials: J
authors.lastname: Fan
authors.affiliation: Beijing Key Laboratory for Agricultural Application and New Technique, Plant Science and Technology College, Beijing University of Agriculture, Beijing, 102206, China.
authors.forename: Canli
authors.initials: C
authors.lastname: Hu
authors.affiliation: Beijing Key Laboratory for Agricultural Application and New Technique, Plant Science and Technology College, Beijing University of Agriculture, Beijing, 102206, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Yang
authors.affiliation: Beijing Key Laboratory for Agricultural Application and New Technique, Plant Science and Technology College, Beijing University of Agriculture, Beijing, 102206, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Qi
authors.affiliation: Beijing Key Laboratory for Agricultural Application and New Technique, Plant Science and Technology College, Beijing University of Agriculture, Beijing, 102206, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Chen
authors.affiliation: Biological Science and Engineering College, Beijing University of Agriculture, Beijing, 102206, China.
authors.forename: Fukuan
authors.initials: F
authors.lastname: Zhao
authors.affiliation: Beijing Key Laboratory for Agricultural Application and New Technique, Plant Science and Technology College, Beijing University of Agriculture, Beijing, 102206, China wangshaohui@bua.edu.cn.
authors.forename: Shaohui
authors.initials: S
authors.lastname: Wang
mesh_terms: D000818:Animals; D003517:Cyclopentanes / Q000378:metabolism; D018551:Solanum lycopersicum / Q000235:genetics* / Q000378:metabolism / Q000469:parasitology*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D054883:Oxylipins / Q000378:metabolism; D010935:Plant Diseases / Q000469:parasitology; D010937:Plant Growth Regulators / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014431:Tylenchoidea / Q000502:physiology*
chemical_list: D003517:Cyclopentanes; D035683:MicroRNAs; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D018749:RNA, Plant; D014157:Transcription Factors; C011006:jasmonic acid
keywords: Deep sequencing; jasmonic acid; miR319/TCP4; miRNAs; root-knot nematode; tomato.