title: Rapid method for the determination of 14 isoflavones in food using UHPLC coupled to photo diode array detection.
abstract: A rapid method for the determination of 14 types of isoflavones in food using ultra-high performance liquid chromatography (UHPLC) was validated in terms of precision, accuracy, sensitivity and linearity. The UHPLC separation was performed on a reverse-phase C18 column (particle size 2 μm, i.d. 2 mm, length 100 mm) using a photo diode array detector that was fixed to 260 nm. The limits of detection and quantification of the UHPLC analyses ranged from 0.03 to 0.33 mg kg(-1). The intra-day and inter-day precision of the individual isoflavones were less than 11.77% and calibration curves exhibited good linearity (r(2) = 0.99) within the tested ranges. These results suggest that the rapid method used in this study could be available to determine of 14 types of isoflavones in a variety of food such as soy bean, black bean, red bean and soybean paste.
authors.affiliation: Food Analysis Center, Korea Food Research Institute, 1201-62, Anyangpangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi Province 463-746, Republic of Korea; Department of Food Biotechnology, University of Science & Technology, 217, Gajeong-ro, Yuseong-gu, Daejeon 305-350, Republic of Korea.
authors.forename: You-Shin
authors.initials: YS
authors.lastname: Shim
authors.affiliation: Food Analysis Center, Korea Food Research Institute, 1201-62, Anyangpangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi Province 463-746, Republic of Korea.
authors.forename: Won-Jin
authors.initials: WJ
authors.lastname: Yoon
authors.affiliation: Food Analysis Center, Korea Food Research Institute, 1201-62, Anyangpangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi Province 463-746, Republic of Korea.
authors.forename: Jin-Bong
authors.initials: JB
authors.lastname: Hwang
authors.affiliation: College of Life Sciences and Biotechnology, Korea University, Anam-dong, Seongbuk-gu, Seoul 136-701, Republic of Korea.
authors.forename: Hyun-Jin
authors.initials: HJ
authors.lastname: Park
authors.affiliation: Food Analysis Center, Korea Food Research Institute, 1201-62, Anyangpangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi Province 463-746, Republic of Korea.
authors.forename: Dongwon
authors.initials: D
authors.lastname: Seo
authors.affiliation: Food Analysis Center, Korea Food Research Institute, 1201-62, Anyangpangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi Province 463-746, Republic of Korea; Department of Food Biotechnology, University of Science & Technology, 217, Gajeong-ro, Yuseong-gu, Daejeon 305-350, Republic of Korea. Electronic address: jhkfri@kfri.re.kr.
authors.forename: Jaeho
authors.initials: J
authors.lastname: Ha
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D007529:Isoflavones / Q000032:analysis* / Q000737:chemistry; D057230:Limit of Detection; D015203:Reproducibility of Results; D045730:Soy Foods / Q000032:analysis*; D013025:Glycine max / Q000737:chemistry*; D013997:Time Factors
chemical_list: D007529:Isoflavones
keywords: Isoflavone; Method validation; Rapid method; UHPLC