title: Diagnosis of Ramularia collo-cygni and Rhynchosporium spp. in Barley.
abstract: Ramularia leaf spot and Rhynchosporium leaf scald are two of the major diseases of barley crops in cooler temperate countries. The methods below are aimed at the identification and quantification of fungal DNA in leaf samples but can also be used for pathogen detection from seed or DNA extracted from environmental samplers. The methods describe in detail two individual quantitative PCR tests. The successful multiplexing of assays will lead to faster throughput of samples.
authors.affiliation: Crop and Soils Systems, SRUC Edinburgh Campus, King's Buildings, West Mains Road, Edinburgh, EH9 3JG, UK, neil.havis@sruc.ac.uk.
authors.forename: Neil
authors.initials: N
authors.lastname: Havis
authors.forename: James
authors.initials: J
authors.lastname: Fountaine
authors.forename: Kalina
authors.initials: K
authors.lastname: Gorniak
authors.forename: Linda
authors.initials: L
authors.lastname: Paterson
authors.forename: Jeanette
authors.initials: J
authors.lastname: Taylor
mesh_terms: D001203:Ascomycota / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D004271:DNA, Fungal / Q000032:analysis* / Q000235:genetics; D001467:Hordeum / Q000235:genetics / Q000382:microbiology*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004271:DNA, Fungal