title: Youngiasides A and C Isolated from Youngia denticulatum Inhibit UVB-Induced MMP Expression and Promote Type I Procollagen Production via Repression of MAPK/AP-1/NF-κB and Activation of AMPK/Nrf2 in HaCaT Cells and Human Dermal Fibroblasts.
abstract: This study investigated the effects of youngiaside A (YA), youngiaside C (YC), and Youngia denticulatum extract (YDE) on extrinsic aging and assessed its molecular mechanisms in UVB-irradiated HaCaT keratinocytes and human dermal fibroblasts (HDFs). The results showed that YA, YC, and YDE decreased matrix metalloproteinase (MMP) expression and production in HaCaT cell and HDFs and increased collagen expression and production in HDFs. In addition, YA, YC, and YDE significantly increased antioxidant enzyme expression, thereby down-regulating UVB-induced reactive oxygen species (ROS) production and ROS-induced mitogen-activated protein kinase (MAPK) and activator protein-1 (AP-1) signaling in HaCaT cells. Furthermore, YA, YC, and YDE reduced phosphorylation of IκBα and IKKα/β, blocked nuclear factor-κB (NF-κB) p65 nuclear translocation, and strongly suppressed pro-inflammatory mediators. Finally, YA, YC, and YDE augmented UVB-induced adenosine monophosphate activated protein kinase (AMPK) phosphorylation and YA and YC did not inhibit MMP-1 production in AMPK inhibitor or nuclear factor-erythroid 2-related factor-2 (Nrf2) siRNA-treated HaCaT cells. The results suggest that these compounds could be potential therapeutic agents for prevention and treatment of skin photoaging.
authors.affiliation: Natural Products Research Center, Korea Institute of Science and Technology, Gangneung, Gangwon-do 210-340, Republic of Korea.
authors.forename: Myungsuk
authors.initials: M
authors.lastname: Kim
authors.affiliation: Natural Products Research Center, Korea Institute of Science and Technology, Gangneung, Gangwon-do 210-340, Republic of Korea.
authors.forename: Young Gyun
authors.initials: YG
authors.lastname: Park
authors.affiliation: Natural Products Research Center, Korea Institute of Science and Technology, Gangneung, Gangwon-do 210-340, Republic of Korea.
authors.forename: Hee-Ju
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Natural Products Research Center, Korea Institute of Science and Technology, Gangneung, Gangwon-do 210-340, Republic of Korea.
authors.forename: Sue Ji
authors.initials: SJ
authors.lastname: Lim
authors.affiliation: Natural Products Research Center, Korea Institute of Science and Technology, Gangneung, Gangwon-do 210-340, Republic of Korea.
authors.forename: Chu Won
authors.initials: CW
authors.lastname: Nho
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D019659:Asteraceae / Q000737:chemistry*; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis; D005347:Fibroblasts / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D020782:Matrix Metalloproteinases / Q000235:genetics* / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D051267:NF-E2-Related Factor 2 / Q000235:genetics / Q000378:metabolism; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D011347:Procollagen / Q000096:biosynthesis*; D015398:Signal Transduction / Q000187:drug effects* / Q000528:radiation effects; D012867:Skin / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism / Q000528:radiation effects; D013473:Sunscreening Agents / Q000494:pharmacology*; D018808:Transcription Factor AP-1 / Q000235:genetics / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D024042:Collagen Type I; D051267:NF-E2-Related Factor 2; D016328:NF-kappa B; C495635:NFE2L2 protein, human; D010936:Plant Extracts; D011347:Procollagen; D013473:Sunscreening Agents; D018808:Transcription Factor AP-1; D020928:Mitogen-Activated Protein Kinases; D055372:AMP-Activated Protein Kinases; D020782:Matrix Metalloproteinases
keywords: MMPs; UV; Youngia denticulatum; type I procollagen; youngiaside