title: TANG1, Encoding a Symplekin_C Domain-Contained Protein, Influences Sugar Responses in Arabidopsis.
abstract: Sugars not only serve as energy and cellular carbon skeleton but also function as signaling molecules regulating growth and development in plants. Understanding the molecular mechanisms in sugar signaling pathways will provide more information for improving plant growth and development. Here, we describe a sugar-hypersensitive recessive mutant, tang1. Light-grown tang1 mutants have short roots and increased starch and anthocyanin contents when grown on high-sugar concentration medium. Dark-grown tang1 plants exhibit sugar-hypersensitive hypocotyl elongation and enhanced dark development. The tang1 mutants also show an enhanced response to abscisic acid but reduced response to ethylene. Thus, tang1 displays a range of alterations in sugar signaling-related responses. The TANG1 gene was isolated by a map-based cloning approach and encodes a previously uncharacterized unique protein with a predicted Symplekin tight-junction protein C terminus. Expression analysis indicates that TANG1 is ubiquitously expressed at moderate levels in different organs and throughout the Arabidopsis (Arabidopsis thaliana) life cycle; however, its expression is not affected by high-sugar treatment. Genetic analysis shows that PRL1 and TANG1 have additive effects on sugar-related responses. Furthermore, the mutation of TANG1 does not affect the expression of genes involved in known sugar signaling pathways. Taken together, these results suggest that TANG1, a unique gene, plays an important role in sugar responses in Arabidopsis.
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China (Le.Z., L.S., X.C., Li.Z., Y.X., H.-C.J.);State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (Y.L.); andDepartment of Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom (C.S., M.W.B.) lyzheng@ibcas.ac.cn hcjing@ibcas.ac.cn.
authors.forename: Leiying
authors.identifier: http://orcid.org/0000-0001-8368-4185
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China (Le.Z., L.S., X.C., Li.Z., Y.X., H.-C.J.);State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (Y.L.); andDepartment of Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom (C.S., M.W.B.).
authors.forename: Li
authors.initials: L
authors.lastname: Shang
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China (Le.Z., L.S., X.C., Li.Z., Y.X., H.-C.J.);State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (Y.L.); andDepartment of Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom (C.S., M.W.B.).
authors.forename: Xing
authors.initials: X
authors.lastname: Chen
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China (Le.Z., L.S., X.C., Li.Z., Y.X., H.-C.J.);State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (Y.L.); andDepartment of Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom (C.S., M.W.B.).
authors.forename: Limin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China (Le.Z., L.S., X.C., Li.Z., Y.X., H.-C.J.);State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (Y.L.); andDepartment of Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom (C.S., M.W.B.).
authors.forename: Yan
authors.identifier: http://orcid.org/0000-0002-8434-2715
authors.initials: Y
authors.lastname: Xia
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China (Le.Z., L.S., X.C., Li.Z., Y.X., H.-C.J.);State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (Y.L.); andDepartment of Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom (C.S., M.W.B.).
authors.forename: Caroline
authors.initials: C
authors.lastname: Smith
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China (Le.Z., L.S., X.C., Li.Z., Y.X., H.-C.J.);State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (Y.L.); andDepartment of Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom (C.S., M.W.B.).
authors.forename: Michael W
authors.identifier: http://orcid.org/0000-0001-8264-2354
authors.initials: MW
authors.lastname: Bevan
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China (Le.Z., L.S., X.C., Li.Z., Y.X., H.-C.J.);State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (Y.L.); andDepartment of Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom (C.S., M.W.B.).
authors.forename: Yunhai
authors.identifier: http://orcid.org/0000-0002-0025-4444
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China (Le.Z., L.S., X.C., Li.Z., Y.X., H.-C.J.);State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (Y.L.); andDepartment of Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom (C.S., M.W.B.) lyzheng@ibcas.ac.cn hcjing@ibcas.ac.cn.
authors.forename: Hai-Chun
authors.initials: HC
authors.lastname: Jing
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D000872:Anthocyanins / Q000378:metabolism; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D002241:Carbohydrates / Q000494:pharmacology*; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D002734:Chlorophyll / Q000378:metabolism; D003001:Cloning, Molecular; D005030:Ethylenes / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000528:radiation effects; D017343:Genes, Plant; D005947:Glucose / Q000494:pharmacology; D047908:Intracellular Signaling Peptides and Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008027:Light; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D017434:Protein Structure, Tertiary; D021381:Protein Transport / Q000187:drug effects / Q000528:radiation effects; D036226:Seedlings / Q000187:drug effects / Q000254:growth & development / Q000528:radiation effects; D013213:Starch / Q000378:metabolism; D062725:Tight Junction Proteins / Q000378:metabolism
chemical_list: D000872:Anthocyanins; D029681:Arabidopsis Proteins; D002241:Carbohydrates; D005030:Ethylenes; D047908:Intracellular Signaling Peptides and Proteins; C000606237:TANG1 protein, Arabidopsis; D062725:Tight Junction Proteins; D002734:Chlorophyll; D000040:Abscisic Acid; D013213:Starch; C036216:ethylene; D005947:Glucose