title: Fabrication of chiral amino acid ionic liquid modified magnetic multifunctional nanospheres for centrifugal chiral chromatography separation of racemates.
abstract: As the rapid development of nanotechnology, the magnetic nanospheres modified with special chiral selective ligands show a great potentiality in enantiomeric separation. In this study, magnetic nanospheres modified with task-specific chiral ionic liquid were designed for the separation of chiral amino acids. These modified magnetic nanospheres were effective in a direct chiral separation of five racemic amino acids (D- and L-cysteine, D- and L-arginine, D- and L-leucine, D- and L-glutamine and D- and L-tryptophan). Furthermore, a new online method for complete separation of the enantiomers via the magnetic nanospheres was established with centrifugal chiral chromatography using a spiral tube assembly mounted on a type-J coil planet centrifuge. One kind of chiral compounds, D- and L-tryptophan was resolved well using this method. These results demonstrated that the modified nanospheres display a good chiral recognition ability, and can be used as a potential material for chiral separation of various racemates.
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 North 3th Ring East Road, Chaoyang District, Beijing 100029, China.
authors.forename: Yating
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 North 3th Ring East Road, Chaoyang District, Beijing 100029, China.
authors.forename: Ailin
authors.initials: A
authors.lastname: Tian
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 North 3th Ring East Road, Chaoyang District, Beijing 100029, China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 North 3th Ring East Road, Chaoyang District, Beijing 100029, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Qi
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 North 3th Ring East Road, Chaoyang District, Beijing 100029, China.
authors.forename: Fengkang
authors.initials: F
authors.lastname: Wang
authors.affiliation: Laboratory of Bioseparation Technology, Biochemistry and Biophysics Center, NHLBI, National Institutes of Health, Bldg. 10, Room 8N230, Bethesda, MD 20892, USA.
authors.forename: Ying
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Laboratory of Bioseparation Technology, Biochemistry and Biophysics Center, NHLBI, National Institutes of Health, Bldg. 10, Room 8N230, Bethesda, MD 20892, USA.
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Ito
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 North 3th Ring East Road, Chaoyang District, Beijing 100029, China. Electronic address: weiyun@mail.buct.edu.cn.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wei
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D002498:Centrifugation; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002845:Chromatography; D052578:Ionic Liquids / Q000737:chemistry*; D008024:Ligands; D060328:Magnetic Phenomena; D054118:Nanospheres; D013237:Stereoisomerism; D014364:Tryptophan / Q000737:chemistry
chemical_list: D000596:Amino Acids; D052578:Ionic Liquids; D008024:Ligands; D014364:Tryptophan
keywords: Centrifugal chiral chromatography; Chiral amino acid ionic liquid; Fabrication; Magnetic nanospheres; d- and l-Tryptophan