title: Detection of BCR/ABL Fusion Gene by Hematological and Cytogenetical Analysis in Chronic Myeloid Leukemia Patients in Quetta, Pakistan.
abstract: BACKGROUND
  Chronic myeloid leukemia (CML) is a myeloproliferative disorder of pluripotent stem cells, caused by reciprocal translocation between the long arms of chromosomes 9 and 22, t(9;22)(q34;q11), known as the Philadelphia chromosome.
  
  
  METHODS
  A total of 51 CML patients were recruited in this study. Complete blood counts of all CML patients were performed to find out their total leukocytes, hemoglobin and platelets. FISH was performed for the detection of BCR-ABL fusion and cryptogenic tests using bone marrow samples were performed for the conformation of Ph (9;22)(q34;q11) and variant translocation mechanisms.
  
  
  RESULTS
  In cytogenetic analysis we observed that out of 51 CML patients 40 (88.9%) were Ph positive and 4 (8.88%) had Ph negative chromosomes. Mean values of WBC 134.5 103/μl, hemoglobin 10.44 mg/dl, and platelets 288.6 103/μl were observed in this study.
  
  
  CONCLUSIONS
  In this study, Ph positive translocation between chromosome (9:22)(q34;q11) were observed in 40 (88.9%) CML patients.
authors.affiliation: Department of Biotechnology, Balochistan University of Information Technology, Engineering and Management Sciences, Quetta, Pakistan E-mail : asifjallali@yahoo.com.
authors.forename: Bibi
authors.initials: B
authors.lastname: Tahira
authors.forename: Muhammad
authors.initials: M
authors.lastname: Asif
authors.forename: Samiullah
authors.initials: S
authors.lastname: Khan
authors.forename: Abrar
authors.initials: A
authors.lastname: Hussain
authors.forename: Muhammad Naeem
authors.initials: MN
authors.lastname: Shahwani
authors.forename: Arif
authors.initials: A
authors.lastname: Malik
authors.forename: Syed
authors.initials: S
authors.lastname: Inayatullah
authors.forename: Zafar
authors.initials: Z
authors.lastname: Iqbal
authors.forename: Mahmood
authors.initials: M
authors.lastname: Rasool
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D002892:Chromosomes, Human, Pair 22 / Q000235:genetics; D002899:Chromosomes, Human, Pair 9 / Q000235:genetics; D020732:Cytogenetic Analysis; D005260:Female; D005500:Follow-Up Studies; D016044:Fusion Proteins, bcr-abl / Q000235:genetics*; D006403:Hematologic Tests; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007223:Infant; D007231:Infant, Newborn; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics* / Q000401:mortality / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D010677:Philadelphia Chromosome; D011379:Prognosis; D015996:Survival Rate; D014178:Translocation, Genetic / Q000235:genetics*; D055815:Young Adult
chemical_list: D016044:Fusion Proteins, bcr-abl