title: Global low-frequency motions in protein allostery: CAP as a model system.
abstract: Allostery is a fundamental process by which ligand binding to a protein alters its activity at a distant site. There is considerable evidence that allosteric cooperativity can be communicated by the modulation of protein dynamics without conformational change. The Catabolite Activator Protein (CAP) ofEscherichia coliis an important experimental exemplar for entropically driven allostery. Here we discuss recent experimentally supported theoretical analysis that highlights the role of global low-frequency dynamics in allostery in CAP and identify how allostery arises as a natural consequence of changes in global low-frequency protein fluctuations on ligand binding.
authors.affiliation: Biophysical Sciences Institute, Durham University, Durham, UK ; School of Biological and Biomedical Sciences, Durham University, Durham, UK.
authors.forename: Philip D
authors.initials: PD
authors.lastname: Townsend
authors.affiliation: Biophysical Sciences Institute, Durham University, Durham, UK ; Department of Chemistry, Durham University, Durham, UK ; School of Chemical Engineering and Analytical Sciences, University of Manchester, Manchester, UK.
authors.forename: Thomas L
authors.initials: TL
authors.lastname: Rodgers
authors.affiliation: Biophysical Sciences Institute, Durham University, Durham, UK ; School of Biological and Biomedical Sciences, Durham University, Durham, UK ; Department of Chemistry, Durham University, Durham, UK.
authors.forename: Ehmke
authors.initials: E
authors.lastname: Pohl
authors.affiliation: Biophysical Sciences Institute, Durham University, Durham, UK ; Department of Chemistry, Durham University, Durham, UK.
authors.forename: Mark R
authors.initials: MR
authors.lastname: Wilson
authors.affiliation: Biophysical Sciences Institute, Durham University, Durham, UK ; Department of Chemistry, Durham University, Durham, UK ; Department of Physics, Durham University, Durham, UK.
authors.forename: Tom C B
authors.initials: TC
authors.lastname: McLeish
authors.affiliation: Biophysical Sciences Institute, Durham University, Durham, UK ; School of Biological and Biomedical Sciences, Durham University, Durham, UK.
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Cann
keywords: Allostery; Catabolite activator protein; Dynamics; Elastic network model; Normal modes; Protein