title: Monomeric and oligomeric flavan-3-ols and antioxidant activity of leaves from different Laurus sp.
abstract: The phenolic profile and antioxidant activity of three endemic Laurus sp. from Portugal were analysed. Dried leaves of L. nobilis L., L. azorica (Seub.) Franco, and L. novocanariensis Rivas Mart., Lousã, Fern. Prieto, E. Días, J. C. Costa & C. Aguiar, collected in the mainland and in the Azores and Madeira archipelagos, respectively, were used to prepare different extracts (aqueous, ethanolic and hydroalcoholic). They were studied regarding their DPPH˙ scavenging activity, total phenolic and flavonoid contents, and the main phenolic compounds were identified by HPLC-DAD-ESI-MS/MS. Total flavonoid contents were 30.1, 46.3, and 36.7 mg of epicatechin equivalents per g of sample (dry weight) for L. nobilis, L. azorica and L. novocanariensis, respectively. Epicatechin was the major compound, representing ∼12.1% of total flavan-3-ols in L. nobilis, ∼25.6% in L. azorica, and ∼19.9% in L. novocanariensis. Although all samples presented a similar phenolic profile, significant differences were observed in their total contents and antioxidant activity.
authors.affiliation: REQUIMTE, LAQV/Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, Rua Jorge Viterbo Ferreira, 228, 4050-313, Porto, Portugal. beatoliv@ff.up.pt.
authors.forename: Ana F
authors.initials: AF
authors.lastname: Vinha
authors.forename: Luís F
authors.initials: LF
authors.lastname: Guido
authors.forename: Anabela S G
authors.initials: AS
authors.lastname: Costa
authors.forename: Rita C
authors.initials: RC
authors.lastname: Alves
authors.forename: M Beatriz P P
authors.initials: MB
authors.lastname: Oliveira
mesh_terms: D000975:Antioxidants / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D001394:Azores; D002392:Catechin / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D027442:Laurus / Q000737:chemistry*; D008970:Molecular Weight; D010636:Phenols / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry*; D011174:Portugal; D044945:Proanthocyanidins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D012997:Solvents / Q000737:chemistry; D013045:Species Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet; D053719:Tandem Mass Spectrometry
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D016166:Free Radical Scavengers; D010636:Phenols; D010936:Plant Extracts; D044945:Proanthocyanidins; D012997:Solvents; C404987:flavan-3-ol; D002392:Catechin