title: [Immunogenicity of recombinant Lactobacillus casei expressing VP2 protein of infectious bursal disease virus in chickens].
abstract: In order to determine immunogenicity and protective effect in chickens, we used the IBDV (Infectious bursal disease virus)-Vp2/Lactobacillus casei as antigen transfer system. First, the immunized and control chickens were challenged by IBDV/DQ at lethal dose to determine the protective ratio. Second, chickens were orallyand intranasally vaccinated twice with 10(9) CFU/mL pLA-VP2/L. casei, pLA/L. casei and PBS as negativecontrol and commercial vaccine as positive control. The bursa injury and the lesion score wererecorded post challenge. The level of specific IgG and sIgA in pLA-VP2/L. casei and positive control groups was significantly higher than that in negativecontrol groups. The protection efficacy in pLA-VP2/L. casei oral group was higher than that inintranasal group. The SI. of pLA-VP2/L. casei oral group was significant higher than other groups. The lesion score indicated the pLA-VP2/L. casei was safer than commercial vaccine for bursa. Collectively, the pLA-VP2/L. casei could be a vaccine candidate for IBDV.
authors.forename: Hongli
authors.initials: H
authors.lastname: Lin
authors.forename: Shenda
authors.initials: S
authors.lastname: Hou
authors.forename: Song
authors.initials: S
authors.lastname: Wang
authors.forename: Yupeng
authors.initials: Y
authors.lastname: Wang
authors.forename: Yunyan
authors.initials: Y
authors.lastname: LuanI
authors.forename: Xilin
authors.initials: X
authors.lastname: Hou
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000917:Antibody Formation; D018175:Birnaviridae Infections / Q000517:prevention & control* / Q000662:veterinary; D002645:Chickens; D007243:Infectious bursal disease virus; D007780:Lacticaseibacillus casei; D011201:Poultry Diseases / Q000517:prevention & control*; D011994:Recombinant Proteins / Q000276:immunology; D015678:Viral Structural Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D011994:Recombinant Proteins; C082513:VP2 protein, infectious bursal disease virus; D015678:Viral Structural Proteins; D014765:Viral Vaccines