title: Sequence-specific RNA cleavage by PNA conjugates of the metal-free artificial ribonuclease tris(2-aminobenzimidazole).
abstract: Tris(2-aminobenzimidazole) conjugates with antisense oligonucleotides are effective site-specific RNA cleavers. Their mechanism of action is independent of metal ions. Here we investigate conjugates with peptide nucleic acids (PNA). RNA degradation occurs with similar rates and substrate specificities as in experiments with DNA conjugates we performed earlier. Although aggregation phenomena are observed in some cases, proper substrate recognition is not compromised. While our previous synthesis of 2-aminobenzimidazoles required an HgO induced cyclization step, a mercury free variant is described herein.
authors.affiliation: Institute for Organic Chemistry and Chemical Biology, Goethe Universität Frankfurt, Max-von-Laue-Straße 7, D-60438 Frankfurt am Main, Germany.
authors.forename: Friederike
authors.initials: F
authors.lastname: Danneberg
authors.affiliation: Karolinska Institutet, Department of Biosciences and Nutrition, Novum, SE-141 83 Huddinge, Sweden.
authors.forename: Alice
authors.initials: A
authors.lastname: Ghidini
authors.affiliation: Institute for Organic Chemistry and Chemical Biology, Goethe Universität Frankfurt, Max-von-Laue-Straße 7, D-60438 Frankfurt am Main, Germany.
authors.forename: Plamena
authors.initials: P
authors.lastname: Dogandzhiyski
authors.affiliation: Institute for Organic Chemistry and Chemical Biology, Goethe Universität Frankfurt, Max-von-Laue-Straße 7, D-60438 Frankfurt am Main, Germany.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Kalden
authors.affiliation: Karolinska Institutet, Department of Biosciences and Nutrition, Novum, SE-141 83 Huddinge, Sweden.
authors.forename: Roger
authors.initials: R
authors.lastname: Strömberg
authors.affiliation: Institute for Organic Chemistry and Chemical Biology, Goethe Universität Frankfurt, Max-von-Laue-Straße 7, D-60438 Frankfurt am Main, Germany.
authors.forename: Michael W
authors.initials: MW
authors.lastname: Göbel
keywords: antisense; fluorescence correlation spectroscopy; guanidine; miRNA 20a; peptide nucleic acids