title: Comparison of fibrin adhesives prepared by 3 different methods.
abstract: BACKGROUND
  Fibrin tissue adhesive, which has applications in several areas of medicine, can be prepared by different methods.
  
  
  OBJECTIVE
  To compare fibrin tissue adhesives prepared by 3 different methods.
  
  
  METHODS
  In this prospective experimental laboratory study, fibrin tissue adhesives prepared by the use of plasma fibrinogen (group 1), cryoprecipitation (group 2), and precipitation by ammonium sulfate (group 3) were tested on 15 rabbits and 10 fragments of dura mater. The quality of the clots was assessed in terms of the success of the healing process, local toxicity, graft adhesion capacity, and degree of adhesion of 2 fragments of dura mater produced.
  
  
  RESULTS
  All methods produced a clot with high adhesion and no toxicity, but tensile strength testing revealed that the glue produced from the ammonium sulfate-precipitated clot (group 3) was the strongest, requiring 39 g/cm(2) to separate the fragments as opposed to 23 g/cm(2) for group 2 and 13 g/cm(2) for group 1.
  
  
  CONCLUSIONS
  All methods produced good results as far as clot formation and non-toxicity, but ammonium sulfate precipitation produced the best tensile strength and was thus the most effective method of preparing fibrin tissue adhesive.
authors.affiliation: Medical Resident in Otolaryngology, HC-UFPR (Hospital de Clínicas da Universidade Federal do Paraná).
authors.forename: Juliana Benthien
authors.initials: JB
authors.lastname: Cavichiolo
authors.affiliation: MMSc. Physician Assistant, Department of Otolaryngology, Faculty of Medicine, UFPR; Associate Professor of Otolaryngology.
authors.forename: Maurício
authors.initials: M
authors.lastname: Buschle
authors.affiliation: Otolaryngologist by ABORLCCF.
authors.forename: Bettina
authors.initials: B
authors.lastname: Carvalho
keywords: Biological Therapy; Fibrin; Fibrin Tissue Adhesive; Fibrinogen; Plasma