title: The function of PlcR in Bacillus anthracis vaccine strain A16R.
abstract: Bacillus anthracis, B. thuringiensis and B. cereus are members of the B. cereus group. They share high genetic similarity. Whereas plcR (Phospholipase C regulator) usually encodes a functional pleiotropic activator protein in B. cereus and B. thuringiensis isolates, a characteristic nonsense mutation is found in all B. anthracis strains investigated, making the gene dysfunctional. To study the function of PlcR in B. anthracis, we used the B. cereus CMCC63301 genome as a template and constructed a recombinant expression plasmid pBE2A-plcR, and introduced it into the B. anthracis vaccine strain A16R, and then analyzed the activity of the hemolysin and sphingomyelinase. The results showed that transformation of B. anthracis with plasmid pBE2A-plcR carrying the native B. cereus plcR gene active the expression of sphingomyelinase gene, but did not activate expression of hemolysin genes of B. anthracis A16R.
authors.affiliation: 1. Ministry of Education Key Laboratory for Tropical Biological Resources, Hainan University, Haikou 570228,China; 2. State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing 100071, China;
authors.forename: Xiao-lin
authors.initials: XL
authors.lastname: Jia
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing 100071, China.
authors.forename: Dong-shu
authors.initials: DS
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing 100071, China.
authors.forename: Zhi-qi
authors.initials: ZQ
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing 100071, China.
authors.forename: Er-ling
authors.initials: EL
authors.lastname: Feng
authors.affiliation: 1. Ministry of Education Key Laboratory for Tropical Biological Resources, Hainan University, Haikou 570228,China; 2. Hainan Key Laboratory for Sustainable Utilization of Tropical Bio-resources-State Key Laboratory Incubation Base, Haikou 570228, China;
authors.forename: Ji-ping
authors.initials: JP
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing 100071, China.
authors.forename: Heng-liang
authors.initials: HL
authors.lastname: Wang
authors.affiliation: Ministry of Education Key Laboratory for Tropical Biological Resources, Hainan University, Haikou 570228,China.
authors.forename: Gui-ying
authors.initials: GY
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing 100071, China.
authors.forename: Xian-kai
authors.initials: XK
authors.lastname: Liu
mesh_terms: D001408:Bacillus anthracis / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D001409:Bacillus cereus / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001428:Bacterial Vaccines / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D006460:Hemolysin Proteins / Q000235:genetics / Q000378:metabolism; D013108:Sphingomyelin Phosphodiesterase / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D006460:Hemolysin Proteins; C099480:PlcR protein, Bacillus; D015534:Trans-Activators; D013108:Sphingomyelin Phosphodiesterase