title: Twofold polyketide branching by a stereoselective enzymatic Michael addition.
abstract: The versatility of the branching module of the rhizoxin polyketide synthase was tested in an in vitro enzyme assay with a polyketide mimic and branched (di)methylmalonyl-CoA extender units. Comparison of the products with synthetic reference compounds revealed that the module is able to stereoselectively introduce two branches in one step by a Michael addition-lactonisation sequence, thus expanding the scope of previously studied PKS systems.
authors.affiliation: Department of Biomolecular Chemistry, Leibniz Institute for Natural Product Research and Infection Biology (HKI), Beutenbergstr. 11a, 07745 Jena, Germany. christian.hertweck@leibniz-hki.de.
authors.forename: Daniel
authors.initials: D
authors.lastname: Heine
authors.forename: Srividhya
authors.initials: S
authors.lastname: Sundaram
authors.forename: Tom
authors.initials: T
authors.lastname: Bretschneider
authors.forename: Christian
authors.initials: C
authors.lastname: Hertweck
mesh_terms: D000214:Acyl Coenzyme A / Q000737:chemistry; D000903:Antibiotics, Antineoplastic / Q000737:chemistry; D018942:Macrolides / Q000737:chemistry*; D048630:Polyketide Synthases / Q000737:chemistry*
chemical_list: D000214:Acyl Coenzyme A; D000903:Antibiotics, Antineoplastic; D018942:Macrolides; C015357:methylmalonyl-coenzyme A; D048630:Polyketide Synthases; C041443:rhizoxin