title: Chloroplast Genome Evolution in the Euglenaceae.
abstract: Over the last few years multiple studies have been published outlining chloroplast genomes that represent many of the photosynthetic euglenid genera. However, these genomes were scattered throughout the euglenophyceaean phylogenetic tree, and focused on comparisons with Euglena gracilis. Here, we present a study exclusively on taxa within the Euglenaceae. Six new chloroplast genomes were characterized, those of Cryptoglena skujai, E. gracilis var. bacillaris, Euglena viridis, Euglenaria anabaena, Monomorphina parapyrum, and Trachelomonas volvocina, and added to six previously published chloroplast genomes to determine if trends existed within the family. With this study: at least one genome has now been characterized for each genus, the genomes of different strains from two taxa were characterized to explore intraspecific variability, and a second taxon has been characterized for the genus Monomorphina to examine intrageneric variability. Overall results showed a large amount of variability among the genomes, though a few trends could be identified both within Euglenaceae and within Euglenophyta. In addition, the intraspecific analysis indicated that the similarity of a genome sequence between strains was taxon dependent, and the intrageneric analysis indicated that the majority of the evolutionary changes within the Euglenaceae occurred intergenerically.
authors.affiliation: Department of Plant Biology, Michigan State University, 612 Wilson Rd, Room# 166, Plant Biology Labs, East Lansing, Michigan, 48824, USA.
authors.forename: Matthew S
authors.initials: MS
authors.lastname: Bennett
authors.affiliation: Department of Plant Biology, Michigan State University, 612 Wilson Rd, Room# 166, Plant Biology Labs, East Lansing, Michigan, 48824, USA.
authors.forename: Richard E
authors.initials: RE
authors.lastname: Triemer
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D017124:Conserved Sequence; D018742:DNA, Chloroplast / Q000737:chemistry / Q000235:genetics*; D005056:Euglena gracilis / Q000235:genetics; D016822:Euglenida / Q000145:classification / Q000235:genetics*; D019143:Evolution, Molecular; D054628:Genome, Chloroplast; D058977:Molecular Sequence Annotation; D016366:Open Reading Frames; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D026801:Synteny
chemical_list: D018742:DNA, Chloroplast
keywords: Chloroplast genomics; Cryptoglena skujai; Euglena gracilis var. bacillaris; Euglena viridis; Euglenaria anabaena; Euglenophyta; Genomics; Monomorphina parapyrum; Trachelomonas volvocina