title: [The quantification and significance of muscle segment homeobox gene Msx2, human topoisomerase II-α, HPV16 and VEGF in sinonasal inverted papilloma].
abstract: OBJECTIVE
  To investigate the quantification and significance of Msx2, topoII-α; HPV16 and VEGF in sinonasal inverted papilloma(SNIP), to study the correlation among the four factors,and to discover the relationship between Msx2 and topoII-α in the process of SNIP malignant transfomation.
  
  
  METHODS
  Real-time quantitative Polymerase Chain Reaction (RT-qPCR) was used to detect the expression of Msx2, topoII-α, HPV16 and VEGF in 13 cases of sinonasal inverted papilloma (SNIP), 10 cases of sinonasal squamous cell carcinoma(NSCC) and 10 cases of inflammatory nasal polyp paraffin (INP)tissues. According to the pathology results SNIP were divided into mild dysplasia, moderate dysplasia and severe dysplasia. All the data were analysised by SPSS17. 0, P<0. 05 was refered to statistically significant difference.
  
  
  RESULTS
  The mRNA level of Msx2, topoII-α, VEGF and HPV16 in SNIP, NSCC tissues were significantly higher than in the INP tissues (P<0. 05). The expression differences of Msx2, topoII-α, HPV16 and VEGF mRNA level in SNIP tissues which were divided into three groups according to their pathological results,were all statistically significantly different between any two of the three groups (P< 0. 05). Using Pearson correlation coefficient analysis,we found positive correlation between any two of the mRNA level of Msx2, topoII-α, VEGF and HPV16 (P<0. 05).
  
  
  CONCLUSIONS
  Msx2 and topoII-α may play an important role in the process of SNIP Malignant transformation,which may be new targets for gene therapy of SNIP and NSCC.
authors.forename: Junzheng
authors.initials: J
authors.lastname: Zhang
authors.forename: Yibing
authors.initials: Y
authors.lastname: Yang
authors.forename: Yong
authors.initials: Y
authors.lastname: Tang
authors.forename: Xifang
authors.initials: X
authors.lastname: Wu
authors.forename: Linhai
authors.initials: L
authors.lastname: Cong
authors.forename: Biao
authors.initials: B
authors.lastname: Ruan
mesh_terms: D000951:Antigens, Neoplasm / Q000502:physiology*; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D004250:DNA Topoisomerases, Type II / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000502:physiology*; D005801:Genes, Homeobox; D018398:Homeodomain Proteins / Q000502:physiology*; D052162:Human papillomavirus 16; D006801:Humans; D009669:Nose Neoplasms / Q000235:genetics*; D018308:Papilloma, Inverted / Q000235:genetics*; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A / Q000502:physiology*
chemical_list: D000951:Antigens, Neoplasm; D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; C084119:MSX2 protein; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D004250:DNA Topoisomerases, Type II