title: [A tumor stem cell-specific marker identified by lineage tracing in the intestine].
abstract: Tumor therapies targeting tumor stem cells(TSCs) have been limited. One of the reasons is that TSC markers are often shared by normal stem cells (NSCs), and therapies targeting those marker-positive cells may cause severe injury to normal tissues. To solve the problem, we focused on doublecortin -like kinase 1 (Dclk1). In the normal intestines of Dclk1(creERT2/+); Rosa26(LacZ/+) mice, LacZ-labeled epithelial cells were scattered along villi after tamoxifen injection. In contrast, in Dclk1(creERT2/+); Rosa26(LacZ/+); Apc(Min/+) mice, intestinal tumors were occupied by LacZ-labeled tumor cells, and selective ablation of Dclk1-positive cells using iDTR system resulted in regression of intestinal tumors without apparent damage to the normal intestines. Thus, Dclk1 appeared to be a marker that discriminates TSCs from NSCs in the intestine.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Seno
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Yamaga
authors.forename: Yuki
authors.initials: Y
authors.lastname: Nakanishi
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Chiba
mesh_terms: D000818:Animals; D000969:Antinematodal Agents / Q000627:therapeutic use; D014408:Biomarkers, Tumor / Q000378:metabolism; D019070:Cell Lineage; D006801:Humans; D007414:Intestinal Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D058990:Molecular Targeted Therapy; D014411:Neoplastic Stem Cells / Q000166:cytology / Q000378:metabolism*
chemical_list: D000969:Antinematodal Agents; D014408:Biomarkers, Tumor