title: Fast determination of biogenic amines in beverages by a core-shell particle column.
abstract: A fast and reliable HPLC method for the determination of 11 biogenic amines in beverages has been performed. After pre-column derivatization with dansyl-chloride a Kinetex C18 core-shell particle column (100 mm × 4.6 mm, 2.6 μm particle size) has been employed and the biogenic amines were identified and quantified in a total run time of 13 min with ultraviolet (UV) or fluorescence detection (FLD). Chromatographic conditions such as column temperature (kept at 50 °C), gradient elution and flow rate have been optimized and the method has been tested on red wine and fruit nectar. The proposed method is enhanced in terms of reduced analysis time and eluent consumption with respect of classical HPLC method as to be comparable to UHPLC methods. Green and cost-effective, this method can be used as a quality-control tool for routine quantitative analysis of biogenic amines in beverages for the average laboratory.
authors.affiliation: Laboratory of Commodity Science, Department of Management, Sapienza University of Rome, via del Castro Laurenziano 9, 00161 Rome, Italy. Electronic address: raffaella.preti@uniroma1.it.
authors.forename: Raffaella
authors.initials: R
authors.lastname: Preti
authors.affiliation: Department of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00161 Rome, Italy.
authors.forename: Marta Letizia
authors.initials: ML
authors.lastname: Antonelli
authors.affiliation: Laboratory of Commodity Science, Department of Management, Sapienza University of Rome, via del Castro Laurenziano 9, 00161 Rome, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Bernacchia
authors.affiliation: Laboratory of Commodity Science, Department of Management, Sapienza University of Rome, via del Castro Laurenziano 9, 00161 Rome, Italy.
authors.forename: Giuliana
authors.initials: G
authors.lastname: Vinci
mesh_terms: D001679:Biogenic Amines / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D014920:Wine / Q000032:analysis*
chemical_list: D001679:Biogenic Amines
keywords: Agmatine (PubChem CID: 199); Biogenic amines; Cadaverine (PubChem CID: 273); Core–shell particle column; Dansyl chloride; Ethylamine (PubChem CID: 6341); Fruit nectar; HPLC; Histamine (PubChem CID: 774); Methylamine (PubChem CID: 6329); Putrescine (PubChem CID: 1045); Serotonin (PubChem CID: 5202); Spermidine (PubChem CID: 1102); Spermine (PubChem CID: 1103); Tyramine (PubChem CID: 560); Wine