title: Quantitative lipidomic analysis of plasma and plasma lipoproteins using MALDI-TOF mass spectrometry.
abstract: Knowledge of the plasma lipid composition is essential to clarify the specific roles of different lipid species in various pathophysiological processes. In this study, we developed an analytical strategy combining high-performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD) and off-line coupling with matrix-assisted laser desorption/ionization with time-of-flight mass spectrometry (MALDI-TOF/MS) to determine the composition of plasma and major lipoproteins at two levels, lipid classes and lipid species. We confirmed the suitability of MALDI-TOF/MS as a quantitative measurement tool studying the linearity and repeatability for triglycerides (TG), phosphatidylethanolamine (PE) and phosphatidylcholine (PC). Moreover, data obtained with this method were correlated with other lipid classes and species measurements using currently available technologies. To establish the potential utility of our approach, human plasma very low density- (VLDL), low density- (LDL) and high density- (HDL) lipoproteins from 10 healthy donors were separated using ultracentrifugation, and compositions of nine lipid classes, cholesteryl esters (CE), TG, free cholesterol (FC), PE, phosphatidylinositol (PI), sulfatides (S), PC, lysophosphatidylcholine (LPC) and sphingomyelin (SM), analyzed. In total, 157 lipid species in plasma, 182 in LDL, 171 in HDL, and 148 in VLDL were quantified. The lipidomic profile was consistent with known differences in lipid classes, but also revealed unexpected differences in lipid species distribution of lipoproteins, particularly for LPC and SM. In summary, the methodology developed in this study constitutes a valid approach to determine the lipidomic composition of plasma and lipoproteins.
authors.affiliation: Servicio de Bioquímica Clínica, Hospital Universitario Ramón y Cajal, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain.
authors.forename: Jorge
authors.initials: J
authors.lastname: Serna
authors.affiliation: Servicio de Bioquímica Clínica, Hospital Universitario Ramón y Cajal, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain.
authors.forename: David
authors.initials: D
authors.lastname: García-Seisdedos
authors.affiliation: Servicio de Bioquímica-Investigación, Hospital Universitario Ramón y Cajal, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Alcázar
authors.affiliation: Servicio de Bioquímica-Investigación, Hospital Universitario Ramón y Cajal, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain; CIBER de Fisiología de la Obesidad y Nutrición (CIBERobn), ISCIII, Spain.
authors.forename: Miguel Ángel
authors.initials: MÁ
authors.lastname: Lasunción
authors.affiliation: Servicio de Bioquímica-Investigación, Hospital Universitario Ramón y Cajal, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain; CIBER de Fisiología de la Obesidad y Nutrición (CIBERobn), ISCIII, Spain.
authors.forename: Rebeca
authors.initials: R
authors.lastname: Busto
authors.affiliation: Servicio de Bioquímica Clínica, Hospital Universitario Ramón y Cajal, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain. Electronic address: oscar.pastor@salud.madrid.org.
authors.forename: Óscar
authors.initials: Ó
authors.lastname: Pastor
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D008055:Lipids / Q000097:blood* / Q000302:isolation & purification; D008074:Lipoproteins / Q000097:blood* / Q000302:isolation & purification; D010713:Phosphatidylcholines / Q000097:blood / Q000592:standards; D010714:Phosphatidylethanolamines / Q000097:blood / Q000592:standards; D052616:Solid Phase Extraction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000592:standards; D014280:Triglycerides / Q000097:blood / Q000592:standards
chemical_list: D008055:Lipids; D008074:Lipoproteins; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D014280:Triglycerides; C483858:phosphatidylethanolamine
keywords: Lipid classes; Lipid species; Lipidomics; Lipoproteins; MALDI; Mass spectrometry