title: Methods for RNA profiling of gravi-responding plant tissues.
abstract: Plant transcriptional responses to gravity stimulation by reorientation are among the fastest measured in any tissue or species. Upon reorientation, changes in abundance of specific mRNAs can be measured within seconds or minutes, for plastid or nuclear encoded genes, respectively. Identifying fast gravity-induced transcripts has been made possible by the development of high-throughput technology for qualitative and quantitative RNA analysis. RNA profiling has undergone further rapid development due to its enormous potential in basic sciences and medical applications. We describe here the current and most widely used methods to profile the changes in an entire transcriptome by high-throughput sequencing of RNA fractions (RNAseq) and single gene transcript analysis using real-time quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR).
authors.affiliation: Department of Plant and Microbial Biology, North Carolina State University, Raleigh, NC, USA.
authors.forename: Jyoti
authors.initials: J
authors.lastname: Dalal
authors.forename: Eric
authors.initials: E
authors.lastname: Land
authors.forename: Naresh
authors.initials: N
authors.lastname: Vasani
authors.forename: Luyan
authors.initials: L
authors.lastname: He
authors.forename: Caroline
authors.initials: C
authors.lastname: Smith
authors.forename: Maria
authors.initials: M
authors.lastname: Rodriguez-Welsh
authors.forename: Imara Y
authors.initials: IY
authors.lastname: Perera
authors.forename: Heike
authors.initials: H
authors.lastname: Sederoff
mesh_terms: D020869:Gene Expression Profiling / Q000379:methods*; D018506:Gene Expression Regulation, Plant; D018466:Gravity Sensing / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012313:RNA / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012639:Seeds / Q000235:genetics* / Q000254:growth & development; D059467:Transcriptome / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D012313:RNA