title: Asterosaponins and glycosylated polyhydroxysteroids from the starfish Culcita novaeguineae and their cytotoxic activities.
abstract: Using combined chromatographic methods, two asterosaponins (compounds 1 and 2), including a new compound novaeguinoside E (compound 1), and six glycosylated polyhydroxysteroids (compounds 3-8) were isolated from a methanol extract of the starfish Culcita novaeguineae. Their structures were determined on the basis of spectroscopic data ((1)H and (13)C NMR, HSQC, HMBC, (1)H-(1)H COSY, ROESY, and HRESI-MS) and by comparison with the literature values. The new compound 1 represents the third example of asterosaponins containing the 5α-cholesta-9(1l)-en-3β,6α,20,22-tetraol aglycone. Among isolated compounds, 4-7 exhibited moderate to weak cytotoxic activities against five human cancer cell lines such as Hep-G2 (hepatoma), KB (epidermoid carcinoma), LNCaP (prostate cancer), MCF7 (breast cancer), and SK-Mel2 (melanoma).
authors.affiliation: a Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST) , 18 Hoang Quoc Viet, Caugiay, Hanoi , Vietnam.
authors.forename: Bui Thi
authors.initials: BT
authors.lastname: Ngoan
authors.affiliation: a Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST) , 18 Hoang Quoc Viet, Caugiay, Hanoi , Vietnam.
authors.forename: Tran Thi Hong
authors.initials: TT
authors.lastname: Hanh
authors.affiliation: a Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST) , 18 Hoang Quoc Viet, Caugiay, Hanoi , Vietnam.
authors.forename: Le Thi
authors.initials: le T
authors.lastname: Vien
authors.affiliation: a Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST) , 18 Hoang Quoc Viet, Caugiay, Hanoi , Vietnam.
authors.forename: Chau Ngoc
authors.initials: CN
authors.lastname: Diep
authors.affiliation: a Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST) , 18 Hoang Quoc Viet, Caugiay, Hanoi , Vietnam.
authors.forename: Nguyen Phuong
authors.initials: NP
authors.lastname: Thao
authors.affiliation: b Institute of Biotechnology (IBT), VAST , 18 Hoang Quoc Viet, Caugiay, Hanoi , Vietnam.
authors.forename: Do Thi
authors.initials: do T
authors.lastname: Thao
authors.affiliation: a Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST) , 18 Hoang Quoc Viet, Caugiay, Hanoi , Vietnam.
authors.forename: Nguyen Van
authors.initials: NV
authors.lastname: Thanh
authors.affiliation: a Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST) , 18 Hoang Quoc Viet, Caugiay, Hanoi , Vietnam.
authors.forename: Nguyen Xuan
authors.initials: NX
authors.lastname: Cuong
authors.affiliation: a Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST) , 18 Hoang Quoc Viet, Caugiay, Hanoi , Vietnam.
authors.forename: Nguyen Hoai
authors.initials: NH
authors.lastname: Nam
authors.affiliation: c Institute of Marine Environment and Resources (IMER), VAST , 246 Da Nang, Haiphong , Vietnam.
authors.forename: Do Cong
authors.initials: do C
authors.lastname: Thung
authors.affiliation: a Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST) , 18 Hoang Quoc Viet, Caugiay, Hanoi , Vietnam.
authors.forename: Phan Van
authors.initials: PV
authors.lastname: Kiem
authors.affiliation: d College of Pharmacy, Chungnam National University , Daejeon 305-764 , Republic of Korea.
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
authors.affiliation: a Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST) , 18 Hoang Quoc Viet, Caugiay, Hanoi , Vietnam.
authors.forename: Chau Van
authors.initials: CV
authors.lastname: Minh
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D006031:Glycosylation; D056945:Hep G2 Cells; D006801:Humans; D007624:KB Cells; D008297:Male; D008545:Melanoma; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D013215:Starfish / Q000737:chemistry*; D013256:Steroids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D012503:Saponins; D013256:Steroids; C000604665:novaeguinoside E
keywords: Culcita novaeguineae; Oreasteridae; cytotoxic activity; novaeguinoside E; starfish