title: Validation of a phage-open reading frame typing kit for rapid identification of methicillin-resistant Staphylococcus aureus (MRSA) transmission in a tertiary hospital.
abstract: Surveillance is very important to prevent the nosocomial spread of methicillin-resistant Staphylococcus aureus (MRSA), and infection sources and routes have historically been identified using molecular and epidemiological genotyping with pulsed-field gel electrophoresis. However, phage-open reading frame typing (POT) has recently been developed. Here, we investigated whether POT would be useful to survey MRSA outbreaks and transmission. We therefore applied POT to 91 MRSA isolates detected in cultures from inpatients at our hospital between May and October 2014. Among the 91 isolates, 12 POT types comprising 38 isolated MRSA strains were considered as overlapping. Five of them were detected in different wards, whereas the remaining seven were found in the same ward, including the emergency department. Three of seven POT number 93-155-111 strains were detected in the surgical ward, and all of four POT number 93-157-61 strains were detected in the cardiosurgical ward. These data suggested that transmission of the MRSA strains with the same POT-types from the same wards was nosocomial, and that POT accurately and rapidly identified MRSA strains, which allowed effective control of infection and transmission.
authors.affiliation: Department of Traumatology and Acute Critical Medicine, Osaka University Hospital, Suita, Osaka, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Takahashi
authors.affiliation: Division of Infection Control and Prevention, Osaka University Hospital, Suita, Osaka, Japan ; Division of Infection Control and Prevention, Tohoku Pharmaceutical University Hospital, Sendai, Japan.
authors.forename: Masafumi
authors.initials: M
authors.lastname: Seki
authors.affiliation: Division of Infection Control and Prevention, Osaka University Hospital, Suita, Osaka, Japan.
authors.forename: Norihisa
authors.initials: N
authors.lastname: Yamamoto
authors.affiliation: Division of Infection Control and Prevention, Osaka University Hospital, Suita, Osaka, Japan.
authors.forename: Shigeto
authors.initials: S
authors.lastname: Hamaguchi
authors.affiliation: Department of Traumatology and Acute Critical Medicine, Osaka University Hospital, Suita, Osaka, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ojima
authors.affiliation: Department of Traumatology and Acute Critical Medicine, Osaka University Hospital, Suita, Osaka, Japan.
authors.forename: Tomoya
authors.initials: T
authors.lastname: Hirose
authors.affiliation: Department of Traumatology and Acute Critical Medicine, Osaka University Hospital, Suita, Osaka, Japan.
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Yoshiya
authors.affiliation: Laboratory for Clinical Investigation, Osaka University Hospital, Suita, Osaka, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Toyokawa
authors.affiliation: Laboratory for Clinical Investigation, Osaka University Hospital, Suita, Osaka, Japan.
authors.forename: Isao
authors.initials: I
authors.lastname: Nishi
authors.affiliation: Department of Traumatology and Acute Critical Medicine, Osaka University Hospital, Suita, Osaka, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ogura
authors.affiliation: Department of Traumatology and Acute Critical Medicine, Osaka University Hospital, Suita, Osaka, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Shimazu
authors.affiliation: Division of Infection Control and Prevention, Osaka University Hospital, Suita, Osaka, Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Tomono
keywords: MRSA; POT; active surveillance; nosocomial transmission