title: Ability of human oral microbiota to produce wine odorant aglycones from odourless grape glycosidic aroma precursors.
abstract: Grape aroma precursors are odourless glycosides that represent a natural reservoir of potential active odorant molecules in wines. Since the first step of wine consumption starts in the oral cavity, the processing of these compounds in the mouth could be an important factor in influencing aroma perception. Therefore, the objective of this work has been to evaluate the ability of human oral microbiota to produce wine odorant aglycones from odourless grape glycosidic aroma precursors previously isolated from white grapes. To do so, two methodological approaches involving the use of typical oral bacteria or the whole oral microbiota isolated from human saliva were followed. Odorant aglycones released in the culture mediums were isolated and analysed by HS-SPME-GC/MS. Results showed the ability of oral bacteria to hydrolyse grape aroma precursors, releasing different types of odorant molecules (terpenes, benzenic compounds and lipid derivatives). The hydrolytic activity seemed to be bacteria-dependent and was subject to large inter-individual variability.
authors.affiliation: Instituto de Investigación en Ciencias de la Alimentación (CIAL), CSIC-UAM, CEI UAM+CSIC, C/ Nicolás Cabrera 9, 28049 Madrid, Spain.
authors.forename: Carolina
authors.initials: C
authors.lastname: Muñoz-González
authors.affiliation: Instituto de Investigación en Ciencias de la Alimentación (CIAL), CSIC-UAM, CEI UAM+CSIC, C/ Nicolás Cabrera 9, 28049 Madrid, Spain.
authors.forename: Carolina
authors.initials: C
authors.lastname: Cueva
authors.affiliation: Instituto de Investigación en Ciencias de la Alimentación (CIAL), CSIC-UAM, CEI UAM+CSIC, C/ Nicolás Cabrera 9, 28049 Madrid, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Ángeles Pozo-Bayón
authors.affiliation: Instituto de Investigación en Ciencias de la Alimentación (CIAL), CSIC-UAM, CEI UAM+CSIC, C/ Nicolás Cabrera 9, 28049 Madrid, Spain. Electronic address: victoria.moreno@csic.es.
authors.forename: M
authors.initials: M
authors.lastname: Victoria Moreno-Arribas
mesh_terms: D000328:Adult; D001419:Bacteria / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006027:Glycosides / Q000378:metabolism*; D006801:Humans; D064307:Microbiota; D009055:Mouth / Q000382:microbiology*; D012903:Smell; D027843:Vitis / Q000737:chemistry*; D014920:Wine / Q000032:analysis*
chemical_list: D006027:Glycosides
keywords: 1-Hexanol (PubChem CID: 8103); Glycosidic aroma precursors; Human oral microbiota; Hydrolytic activity; Linalool (PubChem CID: 6549); Wine; α-Terpineol (PubChem CID: 17100); β-Myrcene (PubChem CID: 31253); β-Phenylethyl alcohol (PubChem CID: 6054)