title: Inhibitory Effects of Verrucarin A on Tunicamycin-Induced ER Stress in FaO Rat Liver Cells.
abstract: Endoplasmic reticulum (ER) stress is linked with development and maintenance of cancer, and serves as a therapeutic target for treatment of cancer. Verrucarin A, isolated from the broth of Fusarium sp. F060190, showed potential inhibitory activity on tunicamycin-induced ER stress in FaO rat liver cells. In addition, the compound decreased tunicamycin-induced GRP78 promoter activity in a dose dependent manner without inducing significant inhibition of luciferase activity and cell growth for 6 and 12 h. Moreover, the compound decreased the expression of GRP78, CHOP, XBP-1, and suppressed XBP-1, and reduced phosphorylation of IRE1α in FaO rat liver cells. This evidence suggests for the first time that verrucarin A inhibited tunicamycin-induced ER stress in FaO rat liver cells.
authors.affiliation: LINC Project Group, Daejeon University, Daejeon 300-716, Korea. eunjs75@dju.kr.
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Bae
authors.affiliation: Department of Chemistry, Mokwon University, Daejeon 302-729, Korea. lswdoc@mokwon.ac.kr.
authors.forename: Seung Woong
authors.initials: SW
authors.lastname: Lee
authors.affiliation: Soram Korean Medicine Hospital, M-Tower, Gangnam-Gu, Seoul 135-879, Korea. ss9335@soram.kr.
authors.forename: Sin
authors.initials: S
authors.lastname: Seong
authors.affiliation: Soram Bio-Medicine Research Institute (SBRI), Soram Korean Medicine Hospital, M-Tower, Gangnam-Gu, Seoul 135-879, Korea. williamcho86@soram.kr.
authors.forename: Wonjun
authors.initials: W
authors.lastname: Cho
authors.affiliation: Chemical Biology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yeongudanjiro, Ochang, Cheongwon, Chungbuk 363-883, Korea. jsahn@kribb.re.kr.
authors.forename: Jong Seog
authors.initials: JS
authors.lastname: Ahn
authors.affiliation: Hyehwa-Liberal Arts College, Daejeon University, Daejeon 300-716, Korea. chojo@dju.kr.
authors.forename: Hyun-Sug
authors.initials: HS
authors.lastname: Cho
mesh_terms: D000818:Animals; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D004347:Drug Interactions; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects*; D004722:Endoribonucleases / Q000378:metabolism; D005786:Gene Expression Regulation; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics; D022781:Hepatocytes / Q000187:drug effects* / Q000502:physiology; D009097:Multienzyme Complexes / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D051381:Rats; D000071701:Regulatory Factor X Transcription Factors; D051742:Transcription Factor CHOP / Q000096:biosynthesis / Q000235:genetics; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics; D014255:Trichothecenes / Q000494:pharmacology*; D014415:Tunicamycin / Q000037:antagonists & inhibitors* / Q000494:pharmacology*; D000071717:X-Box Binding Protein 1; D000091342:Endoplasmic Reticulum Chaperone BiP
chemical_list: D004268:DNA-Binding Proteins; D004722:Endoribonucleases; D006360:Heat-Shock Proteins; D009097:Multienzyme Complexes; D017346:Protein Serine-Threonine Kinases; D000071701:Regulatory Factor X Transcription Factors; D051742:Transcription Factor CHOP; D014157:Transcription Factors; D014255:Trichothecenes; D014415:Tunicamycin; D000071717:X-Box Binding Protein 1; C497251:Ddit3 protein, rat; C575026:Ern1 protein, rat; C051684:GRP78 protein, rat; D000091342:Endoplasmic Reticulum Chaperone BiP; C000605545:Xbp1 protein, rat; C005348:muconomycin A
keywords: ER stress; FaO rat liver cells; Fusarium sp. F060190; tunicamycin; verrucarin A