title: Preparation of proteoglycan from salmon nasal cartilage under nondenaturing conditions.
abstract: Salmon nasal cartilage was micronized in ethanol using a rotor-stator homogenizer for the high yield of proteoglycan extraction. This procedure also brought about depressing the degradation of proteoglycan and the contamination of collagens. Proteoglycan was extracted by 4 M magnesium chloride and isolated by anion-exchange chromatography. The gel filtration HPLC and the antibody reactivity showed that the core protein was intact.
authors.affiliation: a Department of Glycotechnology, Center for Advanced Medical Research , Hirosaki University Graduate School of Medicine , Hirosaki , Japan.
authors.forename: Yota
authors.initials: Y
authors.lastname: Tatara
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Suto
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Sasaki
authors.forename: Masahiko
authors.initials: M
authors.lastname: Endo
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry; D000918:Antibody Specificity; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D000431:Ethanol; D015636:Magnesium Chloride; D055171:Nasal Cartilages / Q000737:chemistry*; D011509:Proteoglycans / Q000737:chemistry / Q000302:isolation & purification*; D012474:Salmon; D052616:Solid Phase Extraction / Q000379:methods*; D012997:Solvents
chemical_list: D000906:Antibodies; D011509:Proteoglycans; D012997:Solvents; D015636:Magnesium Chloride; D000431:Ethanol
keywords: cartilage; extraction; magnesium chloride; micronization; proteoglycan