title: Distribution of transient receptor potential melastatin-8-containing nerve fibers in rat oral and craniofacial structures.
abstract: The transient receptor potential melastatin-8 (TRPM8) is a cold and menthol receptor located in the sensory ganglia. Immunohistochemistry for TRPM8 was performed on oral and craniofacial structures of the rat. TRPM8-immunoreactive (-IR) nerve fibers were detected in the oral mucous membrane. In the gingiva, TRPM8-IR nerve fibers were abundant beneath and within crestal and outer epithelia. Such nerve fibers were also common beneath and within taste buds in the incisive papilla. In addition, TRPM8-immunoreactivity was expressed by some taste bud cells in the papilla. Lips, periodontal ligaments and salivary glands as well as masticatory muscles and temporomandibular joints were mostly devoid of TRPM8-IR nerve fibers. A double immunofluorescence study indicated different distribution patterns of nerve fibers containing TRPM8 and calcitonin gene-related peptide in oral and craniofacial tissues. Retrograde tracing method also indicated that TRPM8-IR nerve fibers in the gingiva and incisive papilla originate from small sensory neurons in the trigeminal ganglion. TRPM8 may be associated with cool, cold nociceptive (<around 25°C) and chemoreceptive transmission in the oral mucosa.
authors.affiliation: Division of Operative Dentistry, Graduate School of Dentistry, Tohoku University, 4-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan.
authors.forename: Takehiro
authors.initials: T
authors.lastname: Yajima
authors.affiliation: Division of Oral and Craniofacial Anatomy, Graduate School of Dentistry, Tohoku University, 4-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan. Electronic address: tadasu@dent.tohoku.ac.jp.
authors.forename: Tadasu
authors.initials: T
authors.lastname: Sato
authors.affiliation: Division of Biological Information, Department of Intelligence Science and Technology, Graduate School of Informatics, Kyoto University, Yoshida-honmachi, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hosokawa
authors.affiliation: Department of Clinical Engineering, Kyushu University of Health and Welfare School of Health Science, 1714-1 Yoshino-machi, Nobeoka 882-8508, Japan.
authors.forename: Teruyoshi
authors.initials: T
authors.lastname: Kondo
authors.affiliation: Division of Operative Dentistry, Graduate School of Dentistry, Tohoku University, 4-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Saito
authors.affiliation: Division of Periodontology and Endodontology, Graduate School of Dentistry, Tohoku University, 4-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan.
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Shimauchi
authors.affiliation: Division of Oral and Craniofacial Anatomy, Graduate School of Dentistry, Tohoku University, 4-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ichikawa
mesh_terms: D000818:Animals; D005145:Face; D005881:Gingiva / Q000294:innervation / Q000378:metabolism; D006257:Head; D008046:Lip / Q000294:innervation / Q000378:metabolism; D008297:Male; D008410:Masticatory Muscles / Q000294:innervation / Q000378:metabolism; D009055:Mouth / Q000294:innervation* / Q000378:metabolism*; D009412:Nerve Fibers / Q000378:metabolism*; D010159:Palate / Q000294:innervation / Q000378:metabolism; D010513:Periodontal Ligament / Q000294:innervation / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018015:Receptors, Calcitonin Gene-Related Peptide / Q000378:metabolism; D050053:TRPM Cation Channels / Q000378:metabolism*; D013650:Taste Buds / Q000378:metabolism; D013704:Temporomandibular Joint / Q000294:innervation / Q000378:metabolism; D012668:Trigeminal Ganglion / Q000033:anatomy & histology / Q000378:metabolism
chemical_list: C491562:Crcp protein, rat; D018015:Receptors, Calcitonin Gene-Related Peptide; D050053:TRPM Cation Channels; C498947:Trpm8 protein, rat
keywords: Gingiva; Immunohistochemistry; Incisive papilla; Rat; Transient receptor potential melastatin-8; Trigeminal ganglion