title: Genetic mapping of resistance toFusarium oxysporumf. sp.tulipaein tulip.
abstract: Fusarium oxysporumis a major problem in the production of tulip bulbs. Breeding for resistant cultivars through a conventional approach is a slow process due to the long life cycle of tulip. Until now, marker-assisted selection (MAS) has been hampered by the large genome size and the absence of a genetic map. This study is aimed at construction of the first genetic map for tulip and at the identification of loci associated with resistance toF. oxysporum. A cross-pollinated population of 125 individuals segregating forFusariumresistance was obtained fromTulipa gesneriana"Kees Nelis" andT. fosteriana"Cantata."Fusariumresistance of the mapping population was evaluated through a soil infection test in two consecutive years, and a spot inoculation test in which a green fluorescent protein taggedFusariumstrain was used for inoculation. The genetic maps have been constructed for the parents separately. The genetic map of "Kees Nelis" comprised 342 markers on 27 linkage groups covering 1707 cM, while the map of "Cantata" comprised 300 markers on 21 linkage groups covering 1201 cM. Median distance between markers was 3.9 cM for "Kees Nelis" and 3.1 cM for "Cantata." Six putative quantitative trait loci (QTLs) forFusariumresistance were identified, derived from both parents. QTL2, QTL3, and QTL6 were significant in all disease tests. For the flanking markers of the QTLs, phenotypic means of the two allelic groups, segregating from a parent for such a marker, were significantly different. These markers will be useful for the development of MAS in tulip breeding.
authors.affiliation: Wageningen UR Plant Breeding, Wageningen University and Research Centre, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands ; Plateau Flower Research Centre, Qinghai University, Xining, 810016 Qinghai China.
authors.forename: Nan
authors.initials: N
authors.lastname: Tang
authors.affiliation: Biointeractions and Plant Health, Wageningen University and Research Centre, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
authors.forename: Theo
authors.initials: T
authors.lastname: van der Lee
authors.affiliation: Wageningen UR Plant Breeding, Wageningen University and Research Centre, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands ; Department of Horticulture, Agriculture Faculty, Damascus University, Damascus, Syria.
authors.forename: Arwa
authors.initials: A
authors.lastname: Shahin
authors.affiliation: Biointeractions and Plant Health, Wageningen University and Research Centre, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
authors.forename: Maarten
authors.initials: M
authors.lastname: Holdinga
authors.affiliation: Wageningen UR Plant Breeding, Wageningen University and Research Centre, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
authors.forename: Paul
authors.initials: P
authors.lastname: Bijman
authors.affiliation: Department of Agricultural, Forest and Food Sciences, University of Turin, Largo Paolo Braccini 2, 10095 Grugliasco, TO Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Caser
authors.affiliation: Wageningen UR Plant Breeding, Wageningen University and Research Centre, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
authors.forename: Richard G F
authors.initials: RG
authors.lastname: Visser
authors.affiliation: Wageningen UR Plant Breeding, Wageningen University and Research Centre, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
authors.forename: Jaap M
authors.initials: JM
authors.lastname: van Tuyl
authors.affiliation: Wageningen UR Plant Breeding, Wageningen University and Research Centre, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
authors.forename: Paul
authors.initials: P
authors.lastname: Arens
keywords: Fungal disease; Genetic map; Green fluorescent protein (GFP); Quantitative trait; SNP; Tulipa