title: Moderate alcohol consumption diminishes the development of non-alcoholic fatty liver disease (NAFLD) in ob/ob mice.
abstract: OBJECTIVE
  Using ob/ob mice as a model of non-alcoholic fatty liver disease (NAFLD), we investigated the effect of moderate alcohol intake on the development of NAFLD and molecular mechanisms involved.
  
  
  METHODS
  Ob/ob mice were fed water or ethanol solution (2.5 g/kg body weight/day) for 6 weeks, and markers of liver injury, insulin signalling and adiponectin in visceral adipose tissue were determined.
  
  
  RESULTS
  Whereas bodyweight and the degree of liver steatosis did not differ among ob/ob mouse groups, those consuming ethanol had markedly less macrovesicular hepatic fat accumulation, inflammatory alterations and significantly lower transaminase levels. Despite similarly elevated protein levels of tumour necrosis factor α, protein concentrations of plasminogen activator inhibitor 1 were significantly lower in livers of ob/ob mice consuming ethanol in comparison with controls. The hepato-protective property of moderate alcohol ingestion in ob/ob mice was associated with an induction of the sirtuin-1/adiponectin-signalling cascade in visceral fat tissue and an activation of Akt in the liver. Similar effects of moderate alcohol exposure were also found in vitro in 3T3-L1 and AML-12 cells.
  
  
  CONCLUSIONS
  These data suggest that moderate alcohol intake may diminish the development of NAFLD through sirtuin-1/-adiponectin-dependent signalling cascades.
authors.affiliation: Institute of Nutritional Sciences, SD Model Systems of Molecular Nutrition, Friedrich-Schiller-University Jena, Dornburger Str. 25, 07743, Jena, Germany.
authors.forename: Giridhar
authors.initials: G
authors.lastname: Kanuri
authors.affiliation: Institute of Nutritional Sciences, SD Model Systems of Molecular Nutrition, Friedrich-Schiller-University Jena, Dornburger Str. 25, 07743, Jena, Germany.
authors.forename: Marianne
authors.initials: M
authors.lastname: Landmann
authors.affiliation: Institute of Nutritional Sciences, SD Model Systems of Molecular Nutrition, Friedrich-Schiller-University Jena, Dornburger Str. 25, 07743, Jena, Germany.
authors.forename: Josephine
authors.initials: J
authors.lastname: Priebs
authors.affiliation: Department of Nutritional Medicine (180 a), University of Hohenheim, Stuttgart, Germany.
authors.forename: Astrid
authors.initials: A
authors.lastname: Spruss
authors.affiliation: Department of Nutritional Medicine (180 a), University of Hohenheim, Stuttgart, Germany.
authors.forename: Marina
authors.initials: M
authors.lastname: Löscher
authors.affiliation: Institute of Nutritional Sciences, SD Model Systems of Molecular Nutrition, Friedrich-Schiller-University Jena, Dornburger Str. 25, 07743, Jena, Germany.
authors.forename: Doreen
authors.initials: D
authors.lastname: Ziegenhardt
authors.affiliation: Department of Nutritional Medicine (180 a), University of Hohenheim, Stuttgart, Germany.
authors.forename: Carolin
authors.initials: C
authors.lastname: Röhl
authors.affiliation: Institute of Nutritional Sciences, SD Model Systems of Molecular Nutrition, Friedrich-Schiller-University Jena, Dornburger Str. 25, 07743, Jena, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Degen
authors.affiliation: Institute of Nutritional Sciences, SD Model Systems of Molecular Nutrition, Friedrich-Schiller-University Jena, Dornburger Str. 25, 07743, Jena, Germany. ina.bergheim@uni-jena.de.
authors.forename: Ina
authors.initials: I
authors.lastname: Bergheim
mesh_terms: D016475:3T3 Cells; D052242:Adiponectin / Q000378:metabolism; D000428:Alcohol Drinking; D000818:Animals; D001835:Body Weight; D053453:Caspase 9 / Q000378:metabolism; D002460:Cell Line; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D000431:Ethanol / Q000008:administration & dosage; D005230:Fatty Acids, Nonesterified / Q000097:blood; D007328:Insulin / Q000097:blood; D050152:Intra-Abdominal Fat / Q000378:metabolism; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008820:Mice, Obese; D065626:Non-alcoholic Fatty Liver Disease / Q000517:prevention & control*; D017395:Plasminogen Activator Inhibitor 1 / Q000378:metabolism; D015398:Signal Transduction; D056564:Sirtuin 1 / Q000378:metabolism; D014280:Triglycerides / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D052242:Adiponectin; C499919:Adipoq protein, mouse; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D017395:Plasminogen Activator Inhibitor 1; D014280:Triglycerides; D014409:Tumor Necrosis Factor-alpha; D000431:Ethanol; C506434:Casp9 protein, mouse; D053453:Caspase 9; C470545:Sirt1 protein, mouse; D056564:Sirtuin 1
keywords: Adiponectin; Ethanol; PAI-1; SIRT1; Visceral fat