title: Drug Targeting Based on a New Concept-Targeting Against TLR4 as an Example.
abstract: TLRs are very important players to regulate innate immune responses. TLR4 controls the host defense by sensing an exotic pathogen, such as lipopolysaccharides. At the same time, some endogenous proteins, including HMGB1 and S100A8, could also function to be a ligand to elicit inflammatory reactions. These facts make TLR4 signaling system very complicated. For instance, the application of TLR4 ligands in cancer therapies is desirable for enhancement of anti-tumor immunity in terms of its reparative nature, but undesirable for enhancement of metastatic growth of cancer cells. In this manuscript, in order to make a novel molecular design to disrupt an interaction between TLR4/MD-2 and endogenous ligands, we provide a potential binding style of the TLR4/MD-2 complex with HMGB1 by using their 3D structural data and docking simulations, and also discuss S100A8 binding to TLR4/MD-2.
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Maru
authors.affiliation: Department of Pharmacology, Tokyo Women's Medical University, 8-1 Kawada, Shinjuku-ku, Tokyo 162-8666, Japan. tomitat@research.twmu.ac.jp.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Tomita
authors.forename: Atsuko
authors.initials: A
authors.lastname: Deguchi
authors.forename: Katsuaki
authors.initials: K
authors.lastname: Ieguchi
authors.forename: Morichika
authors.initials: M
authors.lastname: Takita
authors.forename: Fujiko
authors.initials: F
authors.lastname: Tsukahara
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Takemura
authors.forename: Akio
authors.initials: A
authors.lastname: Kitao
authors.forename: Fabian
authors.initials: F
authors.lastname: Gusovsky
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000627:therapeutic use*; D015195:Drug Design; D024243:HMGB1 Protein / Q000378:metabolism; D006801:Humans; D007113:Immunity, Innate / Q000187:drug effects; D007249:Inflammation / Q000188:drug therapy* / Q000276:immunology / Q000378:metabolism; D018836:Inflammation Mediators / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism; D008024:Ligands; D051218:Lymphocyte Antigen 96 / Q000378:metabolism; D062105:Molecular Docking Simulation; D058990:Molecular Targeted Therapy; D011487:Protein Conformation; D015398:Signal Transduction / Q000187:drug effects; D013329:Structure-Activity Relationship; D051197:Toll-Like Receptor 4 / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D024243:HMGB1 Protein; D018836:Inflammation Mediators; D008024:Ligands; D051218:Lymphocyte Antigen 96; D051197:Toll-Like Receptor 4