title: Effect of sesamin on apoptosis and cell cycle arrest in human breast cancer mcf-7 cells.
abstract: Dietary prevention has been known to reduce breast cancer risk. Sesamin is one of the major components in sesame seeds and has been widely studied and proven to have anti-proliferation and anti-angiogenic effects on cancer cells. In this study, the influence of sesamin was tested in the human breast cancer MCF-7 cell line for cell viability (MTT assay) and cell cycling (flow cytometry). Results showed that sesamin dose-dependently (1, 10 and 50 μM) reduced the cell viability and increased LDH release and apoptosis (TUNEL assay). In addition, there was a significant increase of sub-G1 phase arrest in the cell cycle after sesamin treatment. Furthermore, sesamin increased the expression of apoptotic markers of Bax, caspase-3, and cell cycle control proteins, p53 and checkpoint kinase 2. Taken together, these results suggested that sesamin might be used as a dietary supplement for prevention of breast cancer by modulating apoptotic signal pathways and inhibiting tumor cell growth.
authors.affiliation: Department of Life Sciences, National Central University, Jhongli, Taiwan E-mail : kcjeng@gmail.com.
authors.forename: An-Ci
authors.initials: AC
authors.lastname: Siao
authors.forename: Chien-Wei
authors.initials: CW
authors.lastname: Hou
authors.forename: Yung-Hsi
authors.initials: YH
authors.lastname: Kao
authors.forename: Kee-Ching
authors.initials: KC
authors.lastname: Jeng
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D059447:Cell Cycle Checkpoints / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D064447:Checkpoint Kinase 2 / Q000378:metabolism; D004149:Dioxoles / Q000494:pharmacology*; D005260:Female; D006801:Humans; D017705:Lignans / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D000975:Antioxidants; D004149:Dioxoles; D017705:Lignans; D064447:Checkpoint Kinase 2; C578634:CHEK2 protein, human; C054125:sesamin