title: Small nucleolar RNA 78 promotes the tumorigenesis in non-small cell lung cancer.
abstract: BACKGROUND
  Accumulating evidence suggests that dysregulated snoRNA may play a role in the development of malignancy. In the present study, we investigated the role of SNORD78 in the tumorigenesis of non-small cell lung cancer (NSCLC).
  
  
  METHODS
  We determined the expression level of SNORD78 in NSCLC tissues with quantitative real-time PCR and then studied its clinical significance. We explored the biological significance of SNORD78 with gain-and-loss-of-function analyses both in vitro and in vivo.
  
  
  RESULTS
  A great upregulation of SNORD78 was observed in cancer tissues compared to their adjacent normal tissues. Meanwhile, patients with high SNORD78 expression have significantly poorer prognosis than those with low expression. Inhibition of SNORD78 suppressed the proliferation of NSCLC cells via inducing G0/G1 cell cycle arrest and apoptosis while SNORD78 overexpression promoted the cell proliferation. SNORD78 promoted invasion of NSCLC cells via inducing epithelial-mesenchymal-transition (EMT). SNORD78 was also obviously upregulated in cancer stem-like cells and is required for the self-renewal of NSCLC. The oncogenic activity of SNORD78 was also confirmed with in vivo data.
  
  
  CONCLUSIONS
  Our study identified that SNORD78 may be a potential therapeutic target for NSCLC.
authors.affiliation: Department of Medical Oncology, Shanghai Pulmonary Hospital, Tongji University School of Medicine, 507 Zhengmin Road, Shanghai, 200433, People's Republic of China. zhengdiok@aliyun.com.
authors.forename: Di
authors.initials: D
authors.lastname: Zheng
authors.affiliation: Central Lab, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China. jzshmed@163.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Medical Oncology, Shanghai Pulmonary Hospital, Tongji University School of Medicine, 507 Zhengmin Road, Shanghai, 200433, People's Republic of China. 511918514@qq.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Ni
authors.affiliation: Department of Medical Oncology, Shanghai Pulmonary Hospital, Tongji University School of Medicine, 507 Zhengmin Road, Shanghai, 200433, People's Republic of China. jlok@aliyun.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Luo
authors.affiliation: Department of Medical Oncology, Shanghai Pulmonary Hospital, Tongji University School of Medicine, 507 Zhengmin Road, Shanghai, 200433, People's Republic of China. jwshchest@aliyun.com.
authors.forename: Jiying
authors.initials: J
authors.lastname: Wang
authors.affiliation: Central Lab, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China. liangtangchest@163.com.
authors.forename: Liang
authors.initials: L
authors.lastname: Tang
authors.affiliation: Department of Medical Oncology, Shanghai Pulmonary Hospital, Tongji University School of Medicine, 507 Zhengmin Road, Shanghai, 200433, People's Republic of China. lz99@163.com.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Medical Oncology, Shanghai Pulmonary Hospital, Tongji University School of Medicine, 507 Zhengmin Road, Shanghai, 200433, People's Republic of China. jw56chest@aliyun.com.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Medical Oncology, Shanghai Pulmonary Hospital, Tongji University School of Medicine, 507 Zhengmin Road, Shanghai, 200433, People's Republic of China. jxchestsh@aliyun.com.
authors.forename: Jianfang
authors.initials: J
authors.lastname: Xu
authors.affiliation: Central Lab, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China. bsu88@126.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Su
authors.affiliation: Department of Pathology, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai, 200032, People's Republic of China. chestpathology@126.com.
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis; D063646:Carcinogenesis / Q000235:genetics*; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D011379:Prognosis; D020537:RNA, Small Nucleolar; D014162:Transfection
chemical_list: D020537:RNA, Small Nucleolar