title: In vitro growth, cytopathic effects and clearance of monolayers by clinical isolates of Balamuthia mandrillaris in human skin cell cultures.
abstract: Balamuthia mandrillaris is a free-living ameba (FLA) that has been isolated or its DNA identified in soil, dust and water. It causes a fatal central nervous system infection in humans and animals. Although it is environmental as Acanthamoeba and Naegleria fowleri, the two other free-living amebae that also cause CNS infections in humans and other animals, Balamuthia does not feed on bacteria as the other FLA. In the laboratory, it can be grown on a variety of mammalian cell cultures. In this study we examined the ability of three different Balamuthia isolates to grow on several different human skin cell cultures including the WT/A keratinocyte cell cultures. A corneal isolate of Acanthamoeba castellanii was used for comparison.
authors.affiliation: Laboratory of Free-Living Amebae, Division of Foodborne, Waterborne and Environmental Diseases and Division of Scientific Resources, National Center for Environmental and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd, Atlanta, GA 30333, USA; Laboratoire de Parasitologie Mycologie, Hôpital Cochin, Hôpitaux Universitaires Paris Centre, AP-HP, Faculté de Médecine Université Paris Descartes, 27 Rue Fbg Saint Jacques, 75014 Paris, France. Electronic address: helene.yera@cch.aphp.fr.
authors.forename: Hélène
authors.initials: H
authors.lastname: Yera
authors.affiliation: Laboratoire de Parasitologie Mycologie, Hôpital Cochin, Hôpitaux Universitaires Paris Centre, AP-HP, Faculté de Médecine Université Paris Descartes, 27 Rue Fbg Saint Jacques, 75014 Paris, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Dupouy-Camet
authors.affiliation: Laboratory of Free-Living Amebae, Division of Foodborne, Waterborne and Environmental Diseases and Division of Scientific Resources, National Center for Environmental and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd, Atlanta, GA 30333, USA.
authors.forename: Jonathan W
authors.initials: JW
authors.lastname: Jackson
authors.affiliation: Laboratory of Free-Living Amebae, Division of Foodborne, Waterborne and Environmental Diseases and Division of Scientific Resources, National Center for Environmental and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd, Atlanta, GA 30333, USA.
authors.forename: Rama
authors.initials: R
authors.lastname: Sriram
authors.affiliation: Scientific Products and Support Branch, Division of Scientific Resources, National Center for Environmental and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd, Atlanta, GA 30333, USA.
authors.forename: Stacey
authors.initials: S
authors.lastname: Sweat
authors.affiliation: Scientific Products and Support Branch, Division of Scientific Resources, National Center for Environmental and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd, Atlanta, GA 30333, USA.
authors.forename: Jason M
authors.initials: JM
authors.lastname: Goldstein
authors.affiliation: Laboratory of Free-Living Amebae, Division of Foodborne, Waterborne and Environmental Diseases and Division of Scientific Resources, National Center for Environmental and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd, Atlanta, GA 30333, USA.
authors.forename: Govinda S
authors.initials: GS
authors.lastname: Visvesvara
mesh_terms: D048248:Acanthamoeba castellanii / Q000254:growth & development / Q000472:pathogenicity; D000818:Animals; D059611:Balamuthia mandrillaris / Q000254:growth & development* / Q000472:pathogenicity; D002460:Cell Line; D002648:Child; D004730:Endothelium, Vascular / Q000166:cytology / Q000469:parasitology; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000469:parasitology; D006801:Humans; D015603:Keratinocytes / Q000469:parasitology; D008168:Lung / Q000166:cytology / Q000469:parasitology; D010215:Papio; D011247:Pregnancy; D012867:Skin / Q000166:cytology / Q000469:parasitology*; D012987:Soil / Q000469:parasitology
chemical_list: D012987:Soil
keywords: Balamuthia mandrillaris; Cell culture; Cytopathic effect; Human skin