title: A cationic fluorescent polymeric thermometer for the ratiometric sensing of intracellular temperature.
abstract: We developed new cationic fluorescent polymeric thermometers containing both benzothiadiazole and BODIPY units as an environment-sensitive fluorophore and as a reference fluorophore, respectively. The temperature-dependent fluorescence spectra of the thermometers enabled us to perform highly sensitive and practical ratiometric temperature sensing inside living mammalian cells. Intracellular temperatures of non-adherent MOLT-4 (human acute lymphoblastic leukaemia) and adherent HEK293T (human embryonic kidney) cells could be monitored with high temperature resolutions (0.01-1.0 °C) using the new cationic fluorescent polymeric thermometer.
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. seiichi@mol.f.u-tokyo.ac.jp.
authors.forename: Seiichi
authors.initials: S
authors.lastname: Uchiyama
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Tsuji
authors.forename: Kumiko
authors.initials: K
authors.lastname: Ikado
authors.forename: Aruto
authors.initials: A
authors.lastname: Yoshida
authors.forename: Kyoko
authors.initials: K
authors.lastname: Kawamoto
authors.forename: Teruyuki
authors.initials: T
authors.lastname: Hayashi
authors.forename: Noriko
authors.initials: N
authors.lastname: Inada
mesh_terms: D001896:Boron Compounds / Q000737:chemistry*; D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000737:chemistry*; D057809:HEK293 Cells; D006801:Humans; D042541:Intracellular Space / Q000737:chemistry* / Q000502:physiology; D011108:Polymers / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013696:Temperature; D013821:Thermometers
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; D011108:Polymers