title: Differential β3 and β1 Integrin Expression in Bone Marrow and Cortical Bone of Estrogen Deficient Rats.
abstract: Integrin-based (β3 ) attachments to the extracellular matrix (ECM) on osteocyte cell processes have recently been proposed to play an important role in facilitating osteocyte mechanosensation. However, it is not yet known whether integrin expression is altered in the mechanoregulatory osteocytes during osteoporosis. The objective of this study was to test the hypothesis that the expression of integrin-based mechanosensory complexes (β1 and β3 integrins) is altered as a direct response to estrogen deficiency, in an estrogen deficient animal model of osteoporosis. Four weeks post-operatively, immunohistochemistry was used to detect for β1 and β3 integrin subunits in bone tissue and marrow of ovariectomized (OVX; N = 4) and SHAM (N = 4) operated animals. A tartrate resistant acid phosphatase (TRAP) control stain was performed to quantify the presence of osteoclasts in the bone marrow and bone surfaces. Image analysis was performed to quantify expression patterns in different biological compartments, that is, bone marrow, endosteum, and cortical bone. Our results showed that β1 integrins were ubiquitously expressed throughout the bone and marrow, for both OVX and SHAM groups. β3 integrin subunit expression was lower in bone cells from osteoporotic animals compared to controls, whereas β3 expression in marrow cells did not differ significantly between groups. At the endosteum no difference was observed in β3 integrin subunit expression. As expected, the number of osteoclasts was higher in the OVX group validating an imbalance in bone remodeling. We propose that a reduction in β3 integrin expression in osteocytes might impair mechanosensation by bone cells during estrogen deficiency.
authors.affiliation: Biomechanics Research Centre (BMEC), Biomedical Engineering, College of Engineering and Informatics, National University of Ireland Galway, Ireland.
authors.forename: Muriel
authors.initials: M
authors.lastname: Voisin
authors.affiliation: Biomechanics Research Centre (BMEC), Biomedical Engineering, College of Engineering and Informatics, National University of Ireland Galway, Ireland.
authors.forename: Laoise M
authors.initials: LM
authors.lastname: McNamara
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D001853:Bone Marrow / Q000378:metabolism* / Q000503:physiopathology; D016723:Bone Remodeling; D004195:Disease Models, Animal; D004967:Estrogens / Q000172:deficiency*; D005260:Female; D005269:Femur / Q000378:metabolism* / Q000503:physiopathology; D006801:Humans; D007150:Immunohistochemistry; D019012:Integrin beta1 / Q000378:metabolism*; D039661:Integrin beta3 / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism; D040542:Mechanotransduction, Cellular; D010010:Osteoclasts / Q000378:metabolism; D015663:Osteoporosis, Postmenopausal / Q000378:metabolism* / Q000503:physiopathology; D010052:Ovariectomy; D017208:Rats, Wistar; D000071681:Tartrate-Resistant Acid Phosphatase; D013977:Tibia / Q000378:metabolism* / Q000503:physiopathology; D013997:Time Factors
chemical_list: D004967:Estrogens; D019012:Integrin beta1; D039661:Integrin beta3; D007527:Isoenzymes; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase
keywords: bone; immunohistochemistry; integrin; marrow; mechanosensors; osteoblast; osteocyte