title: A two-hour antibiotic susceptibility test by ATP-bioluminescence.
abstract: The antibiotic susceptibility test (AST) in Clinical Microbiology laboratories is still time-consuming, and most procedures take 24h to yield results. In this study, a rapid antimicrobial susceptibility test using ATP-bioluminescence has been developed. The design of method was performed using five ATCC collection strains of known susceptibility. This procedure was then validated against standard commercial methods on 10 strains of enterococci, 10 staphylococci, 10 non-fermenting gram negative bacilli, and 13 Enterobacteriaceae from patients. The agreement obtained in the sensitivity between the ATP-bioluminescence method and commercial methods (E-test, MicroScan and VITEK2) was 100%. In summary, the preliminary results obtained in this work show that the ATP-bioluminescence method could provide a fast and reliable AST in two hours.
authors.affiliation: Departamento de Microbiología, Facultad de Medicina, Universidad de Valladolid, Spain. Electronic address: gmr810@hotmail.com.
authors.forename: Gabriel Alberto
authors.initials: GA
authors.lastname: March Rosselló
authors.affiliation: Servicio de Microbioología y Parasitología, Hospital de Medina del Campo, Spain.
authors.forename: María Cristina
authors.initials: MC
authors.lastname: García-Loygorri Jordán de Urries
authors.affiliation: Departamento de Microbiología, Facultad de Medicina, Universidad de Valladolid, Spain.
authors.forename: María Purificación
authors.initials: MP
authors.lastname: Gutiérrez Rodríguez
authors.affiliation: Departamento de Microbiología, Facultad de Medicina, Universidad de Valladolid, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Simarro Grande
authors.affiliation: Departamento de Microbiología, Facultad de Medicina, Universidad de Valladolid, Spain; Servicio de Microbiología, Hospital Clínico Universitario de Valladolid, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Orduña Domingo
authors.affiliation: Departamento de Microbiología, Facultad de Medicina, Universidad de Valladolid, Spain; Servicio de Microbiología, Hospital Clínico Universitario de Valladolid, Spain.
authors.forename: Miguel Ángel
authors.initials: MÁ
authors.lastname: Bratos Pérez
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004755:Enterobacteriaceae / Q000187:drug effects; D016983:Enterococcus / Q000187:drug effects; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006801:Humans; D008163:Luminescent Measurements; D008826:Microbial Sensitivity Tests / Q000379:methods*; D012016:Reference Values; D012680:Sensitivity and Specificity; D013210:Staphylococcus / Q000187:drug effects; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D000255:Adenosine Triphosphate
keywords: ATP; Antibiograma rápido; Bioluminescence; Bioluminiscencia; Rapid antibiotic susceptibility test