title: Heterologous expression of ACC deaminase from Trichoderma asperellum improves the growth performance of Arabidopsis thaliana under normal and salt stress conditions.
abstract: Transgenic Arabidopsis thaliana plants expressing the 1-aminocyclopropane-1-carboxylate deaminase gene (ACCD) of Trichoderma asperellum ACCC30536 (TaACCD) were created and their growth performance was assessed under normal and salt stress conditions. In order to characterize their growth, root length, root number, fresh weight (FW), relative water content (RWC), seed production, and seed number were measured. Under normal growing condition, all growth parameters except for dry weight (DW) of the transgenic plants increased significantly compared to WT plants. Furthermore, the transgenic line also exhibited higher tolerance and faster growth than WT plants in the presence of 150 mM NaCl. The increased salt stress tolerance of the transgenic plants is attributed to a greater RWC, root weight, root length, root number and FW under salt stress, and to reduced reactive oxygen species (ROS) level, cell death and electrolyte leakage compared to WT plants. The reduction in ROS levels could be explained by increased activity of several antioxidant enzymes, including peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT). Thus, we propose that heterologous expression of TaACCD could be used to improve salt stress tolerance in plants.
authors.affiliation: Key Laboratory of Plant Genetics and Molecular Breeding, College of Life Science and Agronomy, Zhoukou Normal University, ZhouKou 466001, China. Electronic address: zhangfl2007@126.com.
authors.forename: Fuli
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Plant Genetics and Molecular Breeding, College of Life Science and Agronomy, Zhoukou Normal University, ZhouKou 466001, China.
authors.forename: Ju
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Plant Genetics and Molecular Breeding, College of Life Science and Agronomy, Zhoukou Normal University, ZhouKou 466001, China.
authors.forename: Long
authors.initials: L
authors.lastname: Chen
authors.affiliation: Key Laboratory of Plant Genetics and Molecular Breeding, College of Life Science and Agronomy, Zhoukou Normal University, ZhouKou 466001, China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Shi
authors.affiliation: School of Forestry, Northeast Forestry University, Harbin 150040, China. Electronic address: LZHNEFU@126.com.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Lui
authors.affiliation: Key Laboratory of Plant Genetics and Molecular Breeding, College of Life Science and Agronomy, Zhoukou Normal University, ZhouKou 466001, China. Electronic address: lichengwei@zknu.edu.cn.
authors.forename: Chengwei
authors.initials: C
authors.lastname: Li
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D019755:Carbon-Carbon Lyases / Q000096:biosynthesis* / Q000235:genetics; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000235:genetics; D009997:Osmotic Pressure; D030821:Plants, Genetically Modified / Q000201:enzymology* / Q000235:genetics; D012965:Sodium Chloride / Q000494:pharmacology; D014242:Trichoderma / Q000201:enzymology / Q000235:genetics*
chemical_list: D005656:Fungal Proteins; D012965:Sodium Chloride; C033634:1-aminocyclopropane-1-carboxylate deaminase; D019755:Carbon-Carbon Lyases
keywords: ACC deaminase; Growth promotion; Heterologous expression; Salt stress