title: Dissection of the style's response to pollination using transcriptome profiling in self-compatible (Solanum pimpinellifolium) and self-incompatible (Solanum chilense) tomato species.
abstract: BACKGROUND
  Tomato (Solanum lycopersicum) self-compatibility (SC) is defined as self-pollen tubes that can penetrate their own stigma, elongate in the style and fertilize their own ovules. Self-incompatibility (SI) is defined as self-pollen tubes that are prevented from developing in the style. To determine the influence of gene expression on style self-pollination, a transcriptome-wide comparative analysis of SC and SI tomato unpollinated/pollinated styles was performed using RNA-sequencing (RNA-seq) data.
  
  
  RESULTS
  Transcriptome profiles of 24-h unpollination (UP) and self-pollination (P) styles from SC and SI tomato species were generated using high-throughput next generation sequencing. From the comparison of SC self-pollinated and unpollinated styles, 1341 differentially expressed genes (DEGs) were identified, of which 753 were downregulated and 588 were upregulated. From the comparison of SI self-pollinated and unpollinated styles, 804 DEGs were identified, of which 215 were downregulated and 589 were upregulated. Nine gene ontology (GO) terms were enriched significantly in SC and 78 GO terms were enriched significantly in SI. A total of 105 enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were identified in SC and 80 enriched KEGG pathways were identified in SI, among which "Cysteine and methionine metabolism pathway" and "Plant hormone signal transduction pathway" were significantly enriched in SI.
  
  
  CONCLUSIONS
  This study is the first global transcriptome-wide comparative analysis of SC and SI tomato unpollinated/pollinated styles. Advanced bioinformatic analysis of DEGs uncovered the pathways of "Cysteine and methionine metabolism" and "Plant hormone signal transduction", which are likely to play important roles in the control of pollen tubes growth in SI species.
authors.affiliation: Joint Tomato Research Institute, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China. shuainanhai81517@sohu.com.|Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China. shuainanhai81517@sohu.com.
authors.forename: Panfeng
authors.initials: P
authors.lastname: Zhao
authors.affiliation: Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China. zhangld@sjtu.edu.cn.
authors.forename: Lida
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Joint Tomato Research Institute, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China. lxzhao@sjtu.edu.cn.|Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China. lxzhao@sjtu.edu.cn.
authors.forename: Lingxia
authors.initials: L
authors.lastname: Zhao
mesh_terms: D016000:Cluster Analysis; D030561:Databases, Nucleic Acid; D035264:Flowers / Q000187:drug effects / Q000235:genetics*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D063990:Gene Ontology; D018551:Solanum lycopersicum / Q000187:drug effects / Q000235:genetics*; D053858:Metabolic Networks and Pathways / Q000187:drug effects / Q000235:genetics; D058977:Molecular Sequence Annotation; D010937:Plant Growth Regulators / Q000494:pharmacology; D054817:Pollination / Q000187:drug effects / Q000235:genetics*; D060436:Self-Incompatibility in Flowering Plants / Q000187:drug effects / Q000235:genetics*; D017423:Sequence Analysis, RNA
chemical_list: D010937:Plant Growth Regulators