title: IL-15-secreting γδT cells induce memory T cells in experimental allergic encephalomyelitis (EAE) mice.
abstract: With the most recent data suggesting γδT cells as primary producers of the pro-inflammatory autoimmune-associated cytokine, the relationship between γδT cells and Th17 in experimental allergic encephalitis (EAE) mice requires more extensive investigation. By flow cytometry and qPCR, we identified a new subset of IL-15-secreting γδT (γδT15) cells that increased in EAE mice. The capacity of IL-15-secreting γδT cells inducing memory T cells and memory T cells inducing IL-17(+)Th17 was examined by transferring into EAE mice and 7-week-old female nude mice, respectively. We found that γδT15 induced CD44(hi) memory T cells by secreting IL-15. γδT15-induced memory T cells induced EAE by transforming into pathogenic Th17 cells. The data suggest that a new subset of IL-15-secreting γδT cells mediated the production of memory T cells which transformed into pathogenic Th17 cells in EAE mice.
authors.affiliation: Laboratory of Immunology, Institute of Basic Medical Sciences, PO Box 130 (3), Taiping Road #27, Beijing 100850, China.
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Wang
authors.affiliation: Laboratory of Immunology, Institute of Basic Medical Sciences, PO Box 130 (3), Taiping Road #27, Beijing 100850, China; Institute of Immunology, Zhejiang University School of Medicine, Hangzhou 310013, China.
authors.forename: Yinxiang
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Laboratory of Immunology, Institute of Basic Medical Sciences, PO Box 130 (3), Taiping Road #27, Beijing 100850, China; Department of Mephrology, The 307th Hospital of Chinese People's Liberation Army, Beijing 100850, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Liu
authors.affiliation: Laboratory of Immunology, Institute of Basic Medical Sciences, PO Box 130 (3), Taiping Road #27, Beijing 100850, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Xing
authors.affiliation: Laboratory of Immunology, Institute of Basic Medical Sciences, PO Box 130 (3), Taiping Road #27, Beijing 100850, China.
authors.forename: Gencheng
authors.initials: G
authors.lastname: Han
authors.affiliation: Laboratory of Immunology, Institute of Basic Medical Sciences, PO Box 130 (3), Taiping Road #27, Beijing 100850, China.
authors.forename: Guojiang
authors.initials: G
authors.lastname: Chen
authors.affiliation: Laboratory of Immunology, Institute of Basic Medical Sciences, PO Box 130 (3), Taiping Road #27, Beijing 100850, China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Hou
authors.affiliation: Molecular Immunology Section, Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892-1857, USA.
authors.forename: Ivy M
authors.initials: IM
authors.lastname: Dambuza
authors.affiliation: Laboratory of Immunology, Institute of Basic Medical Sciences, PO Box 130 (3), Taiping Road #27, Beijing 100850, China.
authors.forename: Beifen
authors.initials: B
authors.lastname: Shen
authors.affiliation: Laboratory of Immunology, Institute of Basic Medical Sciences, PO Box 130 (3), Taiping Road #27, Beijing 100850, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Laboratory of Immunology, Institute of Basic Medical Sciences, PO Box 130 (3), Taiping Road #27, Beijing 100850, China. Electronic address: yinher2001@yahoo.com.
authors.forename: He
authors.initials: H
authors.lastname: Xiao
authors.affiliation: Laboratory of Immunology, Institute of Basic Medical Sciences, PO Box 130 (3), Taiping Road #27, Beijing 100850, China. Electronic address: wang_renxi@yahoo.com.
authors.forename: Renxi
authors.initials: R
authors.lastname: Wang
mesh_terms: D000818:Animals; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000139:chemically induced / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D005260:Female; D015870:Gene Expression; D018960:Hyaluronan Receptors / Q000235:genetics / Q000276:immunology; D007156:Immunologic Memory / Q000235:genetics*; D016130:Immunophenotyping; D019409:Interleukin-15 / Q000235:genetics* / Q000276:immunology; D020381:Interleukin-17 / Q000235:genetics / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics* / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology / Q000473:pathology; D058504:Th17 Cells / Q000276:immunology* / Q000473:pathology
chemical_list: C476399:Cd44 protein, mouse; D018960:Hyaluronan Receptors; D019409:Interleukin-15; D020381:Interleukin-17; D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments; D016692:Receptors, Antigen, T-Cell, gamma-delta; C445081:myelin oligodendrocyte glycoprotein (35-55)
keywords: EAE; IL-15; Multiple sclerosis; Th17; γδT Cells