title: Development and Characterization of Transcription Factor Gene-Derived Microsatellite (TFGM) Markers in Medicago truncatula and Their Transferability in Leguminous and Non-Leguminous Species.
abstract: Transcription factors (TFs) are critical adaptor molecules that regulate many plant processes by controlling gene expression. The recent increase in the availability of TF data has made TFs a valuable resource for genic functional microsatellite marker development. In the present study, we developed TF gene-derived microsatellite (TFGM) markers for Medicago truncatula and assessed their cross-species transferability. A total of 203 SSRs were identified from 1467 M. truncatula TF coding sequences, 87.68% of which were trinucleotide repeats, followed by mono- (4.93%) and hexanucleotide repeats (1.48%). Further, 142 TFGM markers showed a high level of transferability to the leguminous (55.63%-85.21%) and non-leguminous (28.17%-50.00%) species. Polymorphisms of 27 TFGM markers were evaluated in 44 alfalfa accessions. The allele number per marker ranged from two to eight with an average of 4.41, and the PIC values ranged from 0.08 to 0.84 with an average of 0.60. Considering the high polymorphism, these TFGM markers developed in our study will be valuable for genetic relationship assessments, marker-assisted selection and comparative genomic studies in leguminous and non-leguminous species.
authors.affiliation: State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agricultural Science and Technology, Lanzhou University, Lanzhou 730020, China.
authors.forename: Wenxian
authors.initials: W
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agricultural Science and Technology, Lanzhou University, Lanzhou 730020, China.
authors.forename: Xitao
authors.initials: X
authors.lastname: Jia
authors.affiliation: State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agricultural Science and Technology, Lanzhou University, Lanzhou 730020, China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agricultural Science and Technology, Lanzhou University, Lanzhou 730020, China.
authors.forename: Zhengshe
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agricultural Science and Technology, Lanzhou University, Lanzhou 730020, China. yrwang@lzu.edu.cn.
authors.forename: Yanrong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agricultural Science and Technology, Lanzhou University, Lanzhou 730020, China.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agricultural Science and Technology, Lanzhou University, Lanzhou 730020, China.
authors.forename: Wengang
authors.initials: W
authors.lastname: Xie
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D001483:Base Sequence; D029863:Cicer / Q000235:genetics; D020224:Expressed Sequence Tags; D018014:Gene Transfer Techniques; D005819:Genetic Markers / Q000235:genetics; D018745:Genome, Plant / Q000235:genetics; D000455:Medicago sativa / Q000235:genetics; D046913:Medicago truncatula / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D012275:Oryza / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D017422:Sequence Analysis, DNA; D014026:Nicotiana / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D014157:Transcription Factors
keywords: Medicago truncatula; microsatellite; transcription factor; transferability