title: Sensitive fluorescent assay for copper (II) determination in aqueous solution using copper-specific ssDNA and Sybr Green I.
abstract: This paper reports a fluorescent turn-off assay for sensitive detection of Cu(2+) in an aqueous solution by using a copper-specific ssDNA Cu100 and Sybr Green I. By monitoring the fluorescence changes arose from different interactions of Sybr Green I with Cu100 and Cu100/Cu(2+) complex, the Cu(2+) could be linearly detected from 5.57 to 250 ppb, with a detection limit of 5.57 ppb. The feasibility of this assay was demonstrated by detecting Cu(2+) in certified reference materials and spiked water samples with satisfactory results.
authors.affiliation: School of Environmental Science and Engineering; Key Laboratory of Urban Agriculture (South), Ministry of Agriculture; School of Agriculture and Biology, and Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai 200240, PR China.
authors.forename: Shenshan
authors.initials: S
authors.lastname: Zhan
authors.affiliation: School of Environmental Science and Engineering; Key Laboratory of Urban Agriculture (South), Ministry of Agriculture; School of Agriculture and Biology, and Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai 200240, PR China.
authors.forename: Hanchu
authors.initials: H
authors.lastname: Xu
authors.affiliation: School of Environmental Science and Engineering; Key Laboratory of Urban Agriculture (South), Ministry of Agriculture; School of Agriculture and Biology, and Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai 200240, PR China.
authors.forename: Weilin
authors.initials: W
authors.lastname: Zhang
authors.affiliation: School of Environmental Science and Engineering; Key Laboratory of Urban Agriculture (South), Ministry of Agriculture; School of Agriculture and Biology, and Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai 200240, PR China.
authors.forename: Xuejia
authors.initials: X
authors.lastname: Zhan
authors.affiliation: Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy; School of Liquor & Food Engineering, Guizhou University, Huaxi District, Guiyang 550025, Guizhou, PR China.
authors.forename: Yuangen
authors.initials: Y
authors.lastname: Wu
authors.affiliation: School of Environmental Science and Engineering; Key Laboratory of Urban Agriculture (South), Ministry of Agriculture; School of Agriculture and Biology, and Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai 200240, PR China.
authors.forename: Lumei
authors.initials: L
authors.lastname: Wang
authors.affiliation: School of Environmental Science and Engineering; Key Laboratory of Urban Agriculture (South), Ministry of Agriculture; School of Agriculture and Biology, and Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai 200240, PR China. Electronic address: zhoupei@sjtu.edu.cn.
authors.forename: Pei
authors.initials: P
authors.lastname: Zhou
mesh_terms: D052160:Benzothiazoles; D003300:Copper / Q000032:analysis* / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000737:chemistry; D003959:Diamines; D005456:Fluorescent Dyes / Q000737:chemistry; D009930:Organic Chemicals / Q000737:chemistry; D011804:Quinolines; D012996:Solutions; D013050:Spectrometry, Fluorescence; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000737:chemistry
chemical_list: D052160:Benzothiazoles; D004277:DNA, Single-Stranded; D003959:Diamines; D005456:Fluorescent Dyes; D009930:Organic Chemicals; D011804:Quinolines; D012996:Solutions; D014874:Water Pollutants, Chemical; C098022:SYBR Green I; D003300:Copper
keywords: Copper (II) determination; Copper-specific ssDNA; Fluorescent assay; Sybr Green I