title: Comprehensive analysis of the venom gland transcriptome of the spider Dolomedes fimbriatus.
abstract: A comprehensive transcriptome analysis of an expressed sequence tag (EST) database of the spider Dolomedes fimbriatus venom glands using single-residue distribution analysis (SRDA) identified 7,169 unique sequences. Mature chains of 163 different toxin-like polypeptides were predicted on the basis of well-established methodology. The number of protein precursors of these polypeptides was appreciably numerous than the number of mature polypeptides. A total of 451 different polypeptide precursors, translated from 795 unique nucleotide sequences, were deduced. A homology search divided the 163 mature polypeptide sequences into 16 superfamilies and 19 singletons. The number of mature toxins in a superfamily ranged from 2 to 49, whereas the diversity of the original nucleotide sequences was greater (2-261 variants). We observed a predominance of inhibitor cysteine knot toxin-like polypeptides among the cysteine-containing structures in the analyzed transcriptome bank. Uncommon spatial folds were also found.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences ul. Miklukho-Maklaya, 16/10 , Moscow 117997, Russia.
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Kozlov
authors.affiliation: Scientific Research Institute of Physical-Chemical Medicine of the Federal Medical and Biological Agency of Russian Federation, 1a, Malaya Pirogovskaya st. , Moscow 119435, Russia ; Moscow Institute of Physics and Technology, Dolgoprudny , Moscow Region 141700, Russia.
authors.forename: Vassili N
authors.initials: VN
authors.lastname: Lazarev
authors.affiliation: Scientific Research Institute of Physical-Chemical Medicine of the Federal Medical and Biological Agency of Russian Federation, 1a, Malaya Pirogovskaya st. , Moscow 119435, Russia ; Moscow Institute of Physics and Technology, Dolgoprudny , Moscow Region 141700, Russia.
authors.forename: Elena S
authors.initials: ES
authors.lastname: Kostryukova
authors.affiliation: Scientific Research Institute of Physical-Chemical Medicine of the Federal Medical and Biological Agency of Russian Federation, 1a, Malaya Pirogovskaya st. , Moscow 119435, Russia.
authors.forename: Oksana V
authors.initials: OV
authors.lastname: Selezneva
authors.affiliation: Scientific Research Institute of Physical-Chemical Medicine of the Federal Medical and Biological Agency of Russian Federation, 1a, Malaya Pirogovskaya st. , Moscow 119435, Russia.
authors.forename: Elena A
authors.initials: EA
authors.lastname: Ospanova
authors.affiliation: Scientific Research Institute of Physical-Chemical Medicine of the Federal Medical and Biological Agency of Russian Federation, 1a, Malaya Pirogovskaya st. , Moscow 119435, Russia ; Moscow Institute of Physics and Technology, Dolgoprudny , Moscow Region 141700, Russia.
authors.forename: Dmitry G
authors.initials: DG
authors.lastname: Alexeev
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences ul. Miklukho-Maklaya, 16/10 , Moscow 117997, Russia ; Scientific Research Institute of Physical-Chemical Medicine of the Federal Medical and Biological Agency of Russian Federation, 1a, Malaya Pirogovskaya st. , Moscow 119435, Russia ; Moscow Institute of Physics and Technology, Dolgoprudny , Moscow Region 141700, Russia.
authors.forename: Vadim M
authors.initials: VM
authors.lastname: Govorun
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences ul. Miklukho-Maklaya, 16/10 , Moscow 117997, Russia.
authors.forename: Eugene V
authors.initials: EV
authors.lastname: Grishin
mesh_terms: D000818:Animals; D001483:Base Sequence; D005088:Exocrine Glands / Q000378:metabolism; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling / Q000379:methods; D013111:Spider Venoms / Q000737:chemistry / Q000235:genetics*; D013112:Spiders; D059467:Transcriptome
chemical_list: D013111:Spider Venoms