title: Self-assembled Monolayer Mediated Surface Environment Modification of Poly(vinylpyrrolidone)-Coated Hollow Au-Ag Nanoshells for Enhanced Loading of Hydrophobic Drug and Efficient Multimodal Therapy.
abstract: Hollow Au-Ag bimetallic nanoshell possessing hydrophobic interior space and hydrophilic exterior surface was prepared and its application as a chemo-thermo-gene therapeutic agent based on its high payload of multiple drugs having different water solubility was demonstrated. The multifunctional drug delivery system is based on the hydrophobic interior created by the self-assembled monolayer (SAM) of hexanethiol onto the inner surface of the hollow metallic nanoshells whereas the outer surface was mostly coated by hydrophilic biocompatible polymer. The nanoshells having surface environment modified by hexanethiol SAMs provided high capacity both for hydrophilic DNAzyme (Dz) to induce gene silencing and for hydrophobic SN38 (7-ethyl-10-hydroxycamptothecin), anticancer drug. The release of the loaded Dz and SN38 was independently triggered by an acidic environment and by photothermal temperature elevation upon irradiation, respectively. The chemo-thermo-gene multitherapy based on the present nanoshells having modified surface environment showed high efficacy in quantitative cell-based assays using Huh7 human liver cell containing hepatitis C viral NS3 gene replicon RNA.
authors.affiliation: †Department of Chemistry, Center for RNA Research, Institute for Basic Sciences (IBS), Seoul National University, Seoul 151-747, Republic of Korea.
authors.forename: Hongje
authors.initials: H
authors.lastname: Jang
authors.affiliation: ‡Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Dong-Eun
authors.initials: DE
authors.lastname: Kim
authors.affiliation: †Department of Chemistry, Center for RNA Research, Institute for Basic Sciences (IBS), Seoul National University, Seoul 151-747, Republic of Korea.|§Lemonex Inc., Seoul 151-742, Republic of Korea.
authors.forename: Dal-Hee
authors.initials: DH
authors.lastname: Min
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000737:chemistry* / Q000633:toxicity; D006046:Gold / Q000737:chemistry* / Q000633:toxicity; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D050997:Nanomedicine; D057145:Nanoshells / Q000737:chemistry* / Q000633:toxicity; D011205:Povidone / Q000737:chemistry* / Q000633:toxicity; D012834:Silver / Q000737:chemistry* / Q000633:toxicity; D013499:Surface Properties
chemical_list: D004337:Drug Carriers; D012834:Silver; D006046:Gold; D011205:Povidone
keywords: chemotherapy; drug delivery; hollow nanoshells; photothermal therapy; self-assembled monolayer