title: Anti-inflammatory effect of Impatiens textori Miq. extract via inhibition of NLRP3 inflammasome activation in in vitro and in vivo experimental models.
abstract: BACKGROUND
  Impatiens textori Miq. (I. textori, Balsaminaceae) is a traditional medicinal herb used for centuries to treat several inflammatory related skin infections and allergic disorders in Asian countries.
  
  
  OBJECTIVE
  In this study, we elucidated the effects of whole plant extracts of I. textori on inflammasome activation using in vitro and in vivo models.
  
  
  METHODS
  LPS-stimulated murine bone marrow macrophages were used to study the regulatory effect of I. textori extract (IT) on inflammasome activation. ATP, nigericin and MSU were used as danger-associated molecules to activate the NLRP3 inflammasome. An LPS-induced acute lung injury (ALI) mouse model was used to study the in vivo effect of IT on inflammasome activation.
  
  
  RESULTS
  IT treated at 25, 50, and 100μg/mL concentrations suppressed interleukin-1β secretion through the attenuation of NLRP3 inflammasome activation (p<0.001 at 100μg/mL) leading to the decreased amount of ASC oligomerization and caspase-1 maturation. For the in vivo model, IT inhibited the NLRP3 expression and cell recruitment at the lung tissue in the ALI mouse model.
  
  
  CONCLUSIONS
  IT exhibited potent anti-inflammatory effects via the attenuation of NLRP3 inflammasome activation supporting the traditional claims and may provide a valuable therapeutic strategy in treating various inflammation-related disorders.
authors.affiliation: Department of Biotechnology, College of Biomedical & Health Science, Konkuk University, Chungju, Republic of Korea.
authors.forename: Xiao
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Biotechnology, College of Biomedical & Health Science, Konkuk University, Chungju, Republic of Korea.
authors.forename: Do-Wan
authors.initials: DW
authors.lastname: Shim
authors.affiliation: Department of Biotechnology, College of Biomedical & Health Science, Konkuk University, Chungju, Republic of Korea.
authors.forename: Ji-Won
authors.initials: JW
authors.lastname: Han
authors.affiliation: Department of Biotechnology, College of Biomedical & Health Science, Konkuk University, Chungju, Republic of Korea.
authors.forename: Woo-Young
authors.initials: WY
authors.lastname: Shin
authors.affiliation: Department of Biotechnology, College of Biomedical & Health Science, Konkuk University, Chungju, Republic of Korea.
authors.forename: Eun-Jeong
authors.initials: EJ
authors.lastname: Sim
authors.affiliation: Department of Food Science and Engineering, Seowon University, Cheongju, Republic of Korea.
authors.forename: Myong-Ki
authors.initials: MK
authors.lastname: Kim
authors.affiliation: Department of Biotechnology, College of Biomedical & Health Science, Konkuk University, Chungju, Republic of Korea.
authors.forename: Kang-Hyuck
authors.initials: KH
authors.lastname: Heo
authors.affiliation: Department of Biotechnology, College of Biomedical & Health Science, Konkuk University, Chungju, Republic of Korea.
authors.forename: Young-Kyu
authors.initials: YK
authors.lastname: Kim
authors.affiliation: Department of Biotechnology, College of Biomedical & Health Science, Konkuk University, Chungju, Republic of Korea.
authors.forename: Sushruta
authors.initials: S
authors.lastname: Koppula
authors.affiliation: Division of Biological Science and Technology, Institute of Biomaterials, Yonsei University, Wonju, Republic of Korea.
authors.forename: Tack-Joong
authors.initials: TJ
authors.lastname: Kim
authors.affiliation: Department of Biotechnology, College of Biomedical & Health Science, Konkuk University, Chungju, Republic of Korea.
authors.forename: Tae-Bong
authors.initials: TB
authors.lastname: Kang
authors.affiliation: Department of Biotechnology, College of Biomedical & Health Science, Konkuk University, Chungju, Republic of Korea. Electronic address: kwangho@kku.ac.kr.
authors.forename: Kwang-Ho
authors.initials: KH
authors.lastname: Lee
mesh_terms: D055371:Acute Lung Injury / Q000188:drug therapy / Q000473:pathology; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D002352:Carrier Proteins / Q000378:metabolism; D002460:Cell Line; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D029069:Impatiens / Q000737:chemistry*; D058847:Inflammasomes / Q000378:metabolism; D007249:Inflammation / Q000188:drug therapy* / Q000473:pathology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008519:Medicine, Traditional; D051379:Mice; D008810:Mice, Inbred C57BL; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D000893:Anti-Inflammatory Agents; D002352:Carrier Proteins; D058847:Inflammasomes; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C492716:Nlrp3 protein, mouse; D010936:Plant Extracts
keywords: ALI; Anti-inflammasome; Danger-associated molecules; IL-1β; NLRP3