title: [Regulatory mechanisms of interleukin-1β on the cyclooxygenase-2 system expression of human neuroglioma cells].
abstract: OBJECTIVE
  To explore the molecular mechanisms by which interleukin-1β (IL-1β) regulates the expression of cytosolic phospholipase A2 (cPLA2) and cyclooxygenase-2 (COX-2) in human neuroglioma cell.
  
  
  METHODS
  H4 neuroglioma cells were treated with IL-1β (2.5 µg/L) for different timepoints up to 72 h. For MAPK study, cells were incubated for 1 h with MAPK inhibitors, SB203580 and PD98059 and subsequently stimulated with IL-1β (1 µg/L) for 24 h. Northern and Western blot were used to determine the protein expressions of cPLA2 and COX-2 respectively. And the content of PGE2 in supernatant was determined by enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  A dose of 2.5 µg/L IL-1β induced the protein expressions of cPLA2 and COX-2 and a subsequent release of PGE2 in a time-dependent manner. And the expressions of cPLA2 and COX-2 peaked at 24 h after stimulation (P < 0.05). The expression of PGE2 increased 250 folds after a 72 h culture. Both SB203580 and PD98059 inhibitors reduced IL-1β-induced PGE2 production while SB203580 alone reduced the expressions of both cPLA2 and COX-2.
  
  
  CONCLUSIONS
  IL-1β induces the expressions of cPLA2 and COX-2 and affects COX-2 at the post-translational level by modulating PGE2 production through the signal transduction pathways of p38 and p42/44 MAPKs.
authors.affiliation: Department of Neurosurgery, Rizhao Municipal People's Hospital, Jining Medical College, Rizhao 276826, China; Email: yangyxrz@163.com.
authors.forename: Yunxue
authors.initials: Y
authors.lastname: Yang
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
mesh_terms: D045744:Cell Line, Tumor; D051546:Cyclooxygenase 2; D015232:Dinoprostone; D005910:Glioma; D006801:Humans; D053583:Interleukin-1beta; D019950:Mitogen-Activated Protein Kinase 1; D015398:Signal Transduction
chemical_list: D053583:Interleukin-1beta; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D019950:Mitogen-Activated Protein Kinase 1; D015232:Dinoprostone