title: Building biological foundries for next-generation synthetic biology.
abstract: Synthetic biology is an interdisciplinary field that takes top-down approaches to understand and engineer biological systems through design-build-test cycles. A number of advances in this relatively young field have greatly accelerated such engineering cycles. Specifically, various innovative tools were developed for in silico biosystems design, DNA de novo synthesis and assembly, construct verification, as well as metabolite analysis, which have laid a solid foundation for building biological foundries for rapid prototyping of improved or novel biosystems. This review summarizes the state-of-the-art technologies for synthetic biology and discusses the challenges to establish such biological foundries.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
authors.forename: Ran
authors.initials: R
authors.lastname: Chao
authors.forename: YongBo
authors.initials: Y
authors.lastname: Yuan
authors.forename: HuiMin
authors.initials: H
authors.lastname: Zhao
mesh_terms: D001331:Automation; D004247:DNA / Q000235:genetics; D058615:Synthetic Biology
chemical_list: D004247:DNA