title: Lipid peroxidation and antioxidant protection in girls with type 1 diabetes mellitus during reproductive system development.
abstract: OBJECTIVE
  Type 1 diabetes mellitus (T1D) is found worldwide and is regarded as one of the main risks to human health. The objective of this study was to determine the state of lipid peroxidation (LPO) and antioxidant protection in girls with T1D type considering the stages of reproductive system development.
  
  
  METHODS
  This study enrolled 56 young girls with T1D and 60 healthy girls (control) matched by age. The study population was divided into 3 age groups: prepubertal, adolescent, and juvenile. The state of LPO and antioxidant system was assessed using the coefficient of oxidative stress that represented the ratio of LPO products to general antioxidative blood activity. Spectrophotometric and fluorometric methods were applied.
  
  
  RESULTS
  The results of our study showed increased conjugated diene (CD) and thiobarbituric acid reactant (TBAR) concentrations as well as a decreased reduced glutathione level in prepubertal girls with T1D. Adolescent girls with T1D had a significantly greater CD level and juvenile girls with T1D had a significantly greater TBAR level and lower α-tocopherol concentration than girls in the control group. The greatest coefficient of oxidative stress (1.16) was observed in the prepubertal period.
  
  
  CONCLUSIONS
  The prepubertal period is characterized by the most severe state of lipid peroxidation process-antioxidant protection.
authors.affiliation: Department of Reproductive Pathophysiology, Scientific Centre of Family Health and Human Reproduction Problems, Siberian Branch, Russian Academy of Medical Sciences, Irkutsk, Russian Federation.
authors.forename: Lubov I
authors.initials: LI
authors.lastname: Kolesnikova
authors.affiliation: Department of Reproductive Pathophysiology, Scientific Centre of Family Health and Human Reproduction Problems, Siberian Branch, Russian Academy of Medical Sciences, Irkutsk, Russian Federation.
authors.forename: Marina A
authors.initials: MA
authors.lastname: Darenskaya
authors.affiliation: Department of Reproductive Pathophysiology, Scientific Centre of Family Health and Human Reproduction Problems, Siberian Branch, Russian Academy of Medical Sciences, Irkutsk, Russian Federation. Electronic address: natkor_84@mail.ru.
authors.forename: Natalia V
authors.initials: NV
authors.lastname: Semenova
authors.affiliation: Department of Reproductive Pathophysiology, Scientific Centre of Family Health and Human Reproduction Problems, Siberian Branch, Russian Academy of Medical Sciences, Irkutsk, Russian Federation.
authors.forename: Lyudmila A
authors.initials: LA
authors.lastname: Grebenkina
authors.affiliation: Department of Reproductive Pathophysiology, Scientific Centre of Family Health and Human Reproduction Problems, Siberian Branch, Russian Academy of Medical Sciences, Irkutsk, Russian Federation.
authors.forename: Larisa V
authors.initials: LV
authors.lastname: Suturina
authors.affiliation: Department of Reproductive Pathophysiology, Scientific Centre of Family Health and Human Reproduction Problems, Siberian Branch, Russian Academy of Medical Sciences, Irkutsk, Russian Federation.
authors.forename: Marya I
authors.initials: MI
authors.lastname: Dolgikh
authors.affiliation: Department of Reproductive Pathophysiology, Scientific Centre of Family Health and Human Reproduction Problems, Siberian Branch, Russian Academy of Medical Sciences, Irkutsk, Russian Federation.
authors.forename: Svetlana V
authors.initials: SV
authors.lastname: Gnusina
mesh_terms: D000293:Adolescent; D002648:Child; D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism*; D005260:Female; D005978:Glutathione / Q000097:blood / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation; D018384:Oxidative Stress; D011627:Puberty / Q000097:blood / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D024502:alpha-Tocopherol / Q000097:blood / Q000378:metabolism
chemical_list: D017392:Thiobarbituric Acid Reactive Substances; D005978:Glutathione; D024502:alpha-Tocopherol
keywords: Antioxidative defense; Girls; Oxidative stress; Type 1 diabetes mellitus