title: Rapid and retrievable recording of big data of time-lapse 3D shadow images of microbial colonies.
abstract: We formerly developed an automatic colony count system based on the time-lapse shadow image analysis (TSIA). Here this system has been upgraded and applied to practical rapid decision. A microbial sample was spread on/in an agar plate with 90 mm in diameter as homogeneously as possible. We could obtain the results with several strains that most of colonies appeared within a limited time span. Consequently the number of colonies reached a steady level (Nstdy) and then unchanged until the end of long culture time to give the confirmed value (Nconf). The equivalence of Nstdy and Nconf as well as the difference of times for Nstdy and Nconf determinations were statistically significant at p < 0.001. Nstdy meets the requirement of practical routines treating a large number of plates. The difference of Nstdy and Nconf, if any, may be elucidated by means of retrievable big data. Therefore Nconf is valid for official documentation.
authors.affiliation: Microbio Corporation, 6-6-3 Minamiyoshinari, Aoba-ku, Sendai, Miyagi 989-3204, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ogawa
authors.affiliation: Sendai National College of Technology, Department of Intelligent and Electronic Systems, 4-16-1 Ayashi-Chuo, Aoba-ku, Sendai, Miyagi 989-3128, Japan.
authors.forename: Senshi
authors.initials: S
authors.lastname: Nasu
authors.affiliation: Sendai National College of Technology, Advanced Course of Information Electronic System Engineering, 4-16-1 Ayashi-Chuo, Aoba-ku, Sendai, Miyagi 989-3128, Japan.
authors.forename: Motomu
authors.initials: M
authors.lastname: Takeshige
authors.affiliation: Tokyo University of Agriculture and Technology, Department of Biotechnology and Life Science, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan.
authors.forename: Mikako
authors.initials: M
authors.lastname: Saito
authors.affiliation: Tokyo University of Agriculture and Technology, Department of Biotechnology and Life Science, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Matsuoka
mesh_terms: D001230:Aspergillus / Q000254:growth & development; D001407:Bacillus / Q000254:growth & development; D002176:Candida albicans / Q000254:growth & development; D015169:Colony Count, Microbial / Q000379:methods*; D004926:Escherichia coli / Q000254:growth & development; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008962:Models, Theoretical; D059008:Time-Lapse Imaging / Q000379:methods*