title: Mass spectrometry tools and workflows for revealing microbial chemistry.
abstract: Since the time Van Leeuwenhoek was able to observe microbes through a microscope, an innovation that led to the birth of the field of microbiology, we have aimed to understand how microorganisms function, interact and communicate. The exciting progress in the development of analytical technologies and workflows has demonstrated that mass spectrometry is a very powerful technique for the interrogation of microbiology at the molecular level. In this review, we aim to highlight the available and emerging tools in mass spectrometry for microbial analysis by overviewing the methods and workflow advances for taxonomic identification, microbial interaction, dereplication and drug discovery. We emphasize their potential for future development and point out unsolved problems and future directions that would aid in the analysis of the chemistry produced by microbes.
authors.affiliation: Collaborative Mass Spectrometry Innovation Center, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, California 92093, USA. pdorrestein@ucsd.edu.
authors.forename: Tal
authors.initials: T
authors.lastname: Luzzatto-Knaan
authors.forename: Alexey V
authors.initials: AV
authors.lastname: Melnik
authors.forename: Pieter C
authors.initials: PC
authors.lastname: Dorrestein
mesh_terms: D001419:Bacteria / Q000737:chemistry* / Q000145:classification; D001424:Bacterial Infections / Q000188:drug therapy / Q000382:microbiology; D018407:Bacterial Physiological Phenomena; D055808:Drug Discovery; D004867:Equipment Design; D005658:Fungi / Q000737:chemistry* / Q000145:classification / Q000502:physiology; D006801:Humans; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D056265:Microbial Interactions; D009181:Mycoses / Q000188:drug therapy / Q000382:microbiology; D057188:Workflow