title: Common marmoset CD117+ hematopoietic cells possess multipotency.
abstract: Analysis of the hematopoiesis of non-human primates is important to clarify the evolution of primate-specific hematopoiesis and immune regulation. However, the engraftment and development of the primate hematopoietic system are well-documented only in humans and are not clear in non-human primates. Callithrix jacchus (common marmoset, CM) is a New World monkey with a high rate of pregnancy and small size that lives in closed colonies. As stem cell factor (SCF) is an essential molecule for hematopoietic stem cell development in mice and humans, we focused on CD117, the SCF receptor, and examined whether CD117-expressing cells possess the hematopoietic stem/progenitor cell characteristics of newborn marmoset-derived hematopoietic cells that can develop into T cells and B cells. When CD117(+) cell fractions of the bone marrow were transplanted into immunodeficient NOD (non-obese diabetic)/Shi-scid, common γc-null (NOG) mice, these cells engrafted efficiently in the bone marrow and spleens of the NOG mice. The CD117(+) cells developed into myeloid lineage cells, CD20(+) B cells and CD3(+) T cells, which could express CM cytokines in vivo. The development of B cells did not precede that of T cells. The development of CD8(+) T cells was dominant in NOG mice. The engraftment was comparable for both CD117(+)CD34(+) cells and CD117(+)CD34(-) cells. These results suggest that the CD117(+) cell fraction can differentiate into all three cell lineages, and the development of marmoset immunity in the xenogeneic environment follows diverse developmental pathways compared with human immunity.
authors.affiliation: Department of Molecular Life Science, Tokai University School of Medicine, Isehara, Japan.
authors.forename: Shin
authors.initials: S
authors.lastname: Shimada
authors.affiliation: Division of Molecular Cell Immunology, Advanced Medical Research Center, Nihon University Graduate School of Medical Science, Tokyo, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nunomura
authors.affiliation: Department of Molecular Life Science, Tokai University School of Medicine, Isehara, Japan Department of Hematology, Tokai University School of Medicine, Isehara, Japan.
authors.forename: Shuya
authors.initials: S
authors.lastname: Mori
authors.affiliation: Central Institute for Experimental Animals, Kawasaki, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Suemizu
authors.affiliation: Central Institute for Experimental Animals, Kawasaki, Japan.
authors.forename: Toshio
authors.initials: T
authors.lastname: Itoh
authors.affiliation: Experimental Animals Institute, Hamamatsu University School of Medicine, Shizuoka, Japan.
authors.forename: Shuji
authors.initials: S
authors.lastname: Takabayashi
authors.affiliation: Support Center for Medical Research and Education, Tokai University School of Medicine, Isehara, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Okada
authors.affiliation: Department of Hematology, Tokai University School of Medicine, Isehara, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Yahata
authors.affiliation: Department of Molecular Life Science, Tokai University School of Medicine, Isehara, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Shiina
authors.affiliation: Experimental Animals Institute, Hamamatsu University School of Medicine, Shizuoka, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Katoh
authors.affiliation: Department of Rheumatology and Clinical Immunology, Clinical Research Center for Allergy and Rheumatology, Sagamihara National Hospital, National Hospital Organization, Sagamihara, Japan.
authors.forename: Ryuji
authors.initials: R
authors.lastname: Suzuki
authors.affiliation: Division of Molecular and Clinical Genetics, Department of Molecular Genetics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.
authors.forename: Kenzaburo
authors.initials: K
authors.lastname: Tani
authors.affiliation: Support Center for Medical Research and Education, Tokai University School of Medicine, Isehara, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Ando
authors.affiliation: Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan.
authors.forename: Hideo
authors.initials: H
authors.lastname: Yagita
authors.affiliation: Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan.
authors.forename: Sonoko
authors.initials: S
authors.lastname: Habu
authors.affiliation: Central Institute for Experimental Animals, Kawasaki, Japan.
authors.forename: Erika
authors.initials: E
authors.lastname: Sasaki
authors.affiliation: Department of Molecular Life Science, Tokai University School of Medicine, Isehara, Japan y-kametn@is.icc.u-tokai.ac.jp.
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Kametani
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000954:Antigens, Surface / Q000378:metabolism; D002144:Callithrix; D002454:Cell Differentiation; D000066673:Cell Self Renewal; D005312:Fetal Blood / Q000166:cytology; D006085:Graft Survival; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D006801:Humans; D016130:Immunophenotyping; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism; D051379:Mice; D022423:Myeloid Cells / Q000166:cytology / Q000378:metabolism; D010641:Phenotype; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism*
chemical_list: D000954:Antigens, Surface; D015395:Histocompatibility Antigens Class I; D019009:Proto-Oncogene Proteins c-kit
keywords: NOD/Shi-scid; common γc-null mice; lymphoid lineage; xeno-transplantation