title: Separation of Binding Protein of Celangulin V from the Midgut of Mythimna separata Walker by Affinity Chromatography.
abstract: Celangulin V, an insecticidal compound isolated from the root bark of Chinese bittersweet, can affect the digestive system of insects. However, the mechanism of how Celangulin V induces a series of symptoms is still unknown. In this study, affinity chromatography was conducted through coupling of Celangulin V-6-aminoacetic acid ester to the CNBr-activated Sepharose 4B. SDS-PAGE was used to analyze the collected fraction eluted by Celangulin V. Eight binding proteins (Zinc finger protein, Thioredoxin peroxidase (TPx), Glyceraldehyde 3-phosphate dehydrogenase (GAPDH), SUMO E3 ligase RanBP2, Transmembrane protein 1, Actin, APN and V-ATPase) were obtained and identified by LC/Q-TOF-MS from the midgut of Mythimna separata larvae. The potential of these proteins to serve as target proteins involved in the insecticidal activity of Celangulin V is discussed.
authors.affiliation: Institute of Pesticide Science, College of Plant Protection, Northwest A & F University, Yangling 712100, Shaanxi, China. linalusailboat@163.com.
authors.forename: Lina
authors.initials: L
authors.lastname: Lu
authors.affiliation: Institute of Pesticide Science, College of Plant Protection, Northwest A & F University, Yangling 712100, Shaanxi, China. qzhij@nwsuaf.edu.cn.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Qi
authors.affiliation: College of Science, Northwest A & F University, Yangling 712100, Shaanxi, China. nwzjw@163.com.
authors.forename: Jiwen
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Institute of Pesticide Science, College of Plant Protection, Northwest A & F University, Yangling 712100, Shaanxi, China. wuwenjun@nwsuaf.edu.cn.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Wu
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000302:isolation & purification* / Q000378:metabolism; D002846:Chromatography, Affinity; D041981:Gastrointestinal Tract / Q000378:metabolism; D006241:Haptens / Q000378:metabolism*; D019476:Insect Proteins / Q000302:isolation & purification* / Q000378:metabolism; D007306:Insecticides / Q000378:metabolism*; D007814:Larva; D008024:Ligands; D009036:Moths
chemical_list: D002352:Carrier Proteins; D006241:Haptens; D019476:Insect Proteins; D007306:Insecticides; D008024:Ligands; C514792:celangulin V
keywords: Celangulin V; Mythimna separata Walker; affinity chromatography; binding protein