title: Comparative proteomics and global genome-wide expression data implicate role of ARMC8 in lung cancer.
abstract: BACKGROUND
  Cancer loci comprise heterogeneous cell populations with diverse cellular secretions. Therefore, disseminating cancer-specific or cancer-associated protein antigens from tissue lysates could only be marginally correct, if otherwise not validated against precise standards.
  
  
  METHODS
  In this study, 2DE proteomic profiles were examined from lysates of 13 lung-adenocarcinoma tissue samples and matched against the A549 cell line proteome. A549 matched-cancer-specific hits were analyzed and characterized by MALDI-TOF/MS.
  
  
  RESULTS
  Comparative analysis identified a total of 13 protein spots with differential expression. These proteins were found to be involved in critical cellular functions regulating pyrimidine metabolism, pentose phosphate pathway and integrin signaling. Gene ontology based analysis classified majority of protein hits responsible for metabolic processes. Among these, only a single non-predictive protein spot was found to be a cancer cell specific hit, identified as Armadillo repeat-containing protein 8 (ARMC8). Pathway reconstruction studies showed that ARMC8 lies at the centre of cancer metabolic pathways.
  
  
  CONCLUSIONS
  The findings in this report are suggestive of a regulatory role of ARMC8 in control of proliferation and differentiation in lung adenocarcinomas.
authors.affiliation: Department of Biotechnology, University of Kashmir, Srinagar India E-mail : raies@kashmiruniversity.ac.in.
authors.forename: Asif
authors.initials: A
authors.lastname: Amin
authors.forename: Shoiab
authors.initials: S
authors.lastname: Bukhari
authors.forename: Taseem A
authors.initials: TA
authors.lastname: Mokhdomi
authors.forename: Naveed
authors.initials: N
authors.lastname: Anjum
authors.forename: Asrar H
authors.initials: AH
authors.lastname: Wafai
authors.forename: Zubair
authors.initials: Z
authors.lastname: Wani
authors.forename: Saima
authors.initials: S
authors.lastname: Manzoor
authors.forename: Aabid M
authors.initials: AM
authors.lastname: Koul
authors.forename: Basit
authors.initials: B
authors.lastname: Amin
authors.forename: Qurat Ul
authors.initials: QU
authors.lastname: Ain
authors.forename: Hilal
authors.initials: H
authors.lastname: Qazi
authors.forename: Sumira
authors.initials: S
authors.lastname: Tyub
authors.forename: Ghulam Nabi
authors.initials: GN
authors.lastname: Lone
authors.forename: Raies A
authors.initials: RA
authors.lastname: Qadri
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism*; D051186:Armadillo Domain Proteins / Q000235:genetics / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D016022:Case-Control Studies; D015180:Electrophoresis, Gel, Two-Dimensional; D053263:Gene Regulatory Networks; D015894:Genome, Human; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism*; D053858:Metabolic Networks and Pathways; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014407:Tumor Cells, Cultured
chemical_list: C522393:ARMC8 protein, human; D051186:Armadillo Domain Proteins; D014408:Biomarkers, Tumor; D020543:Proteome