title: An association study between USP34 and polycystic ovary syndrome.
abstract: BACKGROUND
  Polycystic ovary syndrome (PCOS) is a complex multifactor disorder and genetic factors have been implicated in its pathogenesis. Our previous genome-wide association study (GWAS) had identified allele frequencies in several single nucleotide polymorphisms (SNPs) in gene USP34 (Ubiquitin-Specific Protease 34) were significantly different between PCOS cases and controls. This study was aimed to replicate the previous results in another independent cohort.
  
  
  METHODS
  One thousand two hundred eighteen PCOS cases and 1057 controls were recruited. Genotyping of two SNPs (rs17008097 and rs17008940) in USP34 gene were performed by TaqMan-MGB probe assay and genotype-phenotype analysis was conducted subsequently.
  
  
  RESULTS
  The differences of allele or genotype frequencies were not significant statistically between PCOS and controls, even after age and BMI adjustment. For clinical and metabolic features (LH, T and HOMA-IR) analysis in PCOS cases, no statistical differences among three genotypes of rs17008097 and rs17008940 were found. However, rs17008940 was shown to be slightly associated with BMI in PCOS cases rather than in controls, even after age adjustment (TC vs CC P = 0.006, OR = 1.042, 95% CI 1.012-1.073; TT vs CC P = 0.037, OR = 1.050, 95% CI 1.003-1.100).
  
  
  CONCLUSIONS
  USP34 gene polymorphisms (rs17008097 and rs17008940) may not be associated with PCOS in the Han Chinese women.
authors.affiliation: Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Center for Reproductive Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, 200135, China. zsg0108@126.com.|Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, Jinan, China. zsg0108@126.com.|National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Jinan, China. zsg0108@126.com.|The Key laboratory for Reproductive Endocrinology of Ministry of Education, Jinan, China. zsg0108@126.com.|Shandong Provincial Key Laboratory of Reproductive Medicine, Jinan, 250021, China. zsg0108@126.com.
authors.forename: Shigang
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Center for Reproductive Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, 200135, China. tianyesdu@126.com.
authors.forename: Ye
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Department of joint and bone oncology, Provincial Hospital Affiliated to Shandong University, Jinan, 250021, China. zhangweisdu2015@126.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, Jinan, China. xingxiuye@163.com.|National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Jinan, China. xingxiuye@163.com.|The Key laboratory for Reproductive Endocrinology of Ministry of Education, Jinan, China. xingxiuye@163.com.|Shandong Provincial Key Laboratory of Reproductive Medicine, Jinan, 250021, China. xingxiuye@163.com.
authors.forename: Xiuye
authors.initials: X
authors.lastname: Xing
authors.affiliation: Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, Jinan, China. litao8309@126.com.|National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Jinan, China. litao8309@126.com.|The Key laboratory for Reproductive Endocrinology of Ministry of Education, Jinan, China. litao8309@126.com.|Shandong Provincial Key Laboratory of Reproductive Medicine, Jinan, 250021, China. litao8309@126.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.affiliation: Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, Jinan, China. humangenetics2008@hotmail.com.|National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Jinan, China. humangenetics2008@hotmail.com.|The Key laboratory for Reproductive Endocrinology of Ministry of Education, Jinan, China. humangenetics2008@hotmail.com.|Shandong Provincial Key Laboratory of Reproductive Medicine, Jinan, 250021, China. humangenetics2008@hotmail.com.
authors.forename: Hongbin
authors.initials: H
authors.lastname: Liu
authors.affiliation: Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, Jinan, China. htao1568@126.com.|National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Jinan, China. htao1568@126.com.|The Key laboratory for Reproductive Endocrinology of Ministry of Education, Jinan, China. htao1568@126.com.|Shandong Provincial Key Laboratory of Reproductive Medicine, Jinan, 250021, China. htao1568@126.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Huang
authors.affiliation: Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, Jinan, China. yunna_ning@163.com.|National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Jinan, China. yunna_ning@163.com.|The Key laboratory for Reproductive Endocrinology of Ministry of Education, Jinan, China. yunna_ning@163.com.|Shandong Provincial Key Laboratory of Reproductive Medicine, Jinan, 250021, China. yunna_ning@163.com.
authors.forename: Yunna
authors.initials: Y
authors.lastname: Ning
authors.affiliation: Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, Jinan, China. hanzh80@yahoo.com.|National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Jinan, China. hanzh80@yahoo.com.|The Key laboratory for Reproductive Endocrinology of Ministry of Education, Jinan, China. hanzh80@yahoo.com.|Shandong Provincial Key Laboratory of Reproductive Medicine, Jinan, 250021, China. hanzh80@yahoo.com.
authors.forename: Han
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Center for Reproductive Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, 200135, China. chenzijiang@hotmail.com.|Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, Jinan, China. chenzijiang@hotmail.com.|National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Jinan, China. chenzijiang@hotmail.com.|The Key laboratory for Reproductive Endocrinology of Ministry of Education, Jinan, China. chenzijiang@hotmail.com.|Shandong Provincial Key Laboratory of Reproductive Medicine, Jinan, 250021, China. chenzijiang@hotmail.com.
authors.forename: Zi-Jiang
authors.initials: ZJ
authors.lastname: Chen
mesh_terms: D000328:Adult; D044466:Asian People / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D011085:Polycystic Ovary Syndrome / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D064570:Ubiquitin-Specific Proteases / Q000235:genetics*
chemical_list: C584673:USP34 protein, human; D064570:Ubiquitin-Specific Proteases