title: Novel restricted access materials combined to molecularly imprinted polymers for selective solid-phase extraction of organophosphorus pesticides from honey.
abstract: A novel restricted access materials (RAM) combined to molecularly imprinted polymers (MIPs), using malathion as template molecule and glycidilmethacrylate (GMA) as pro-hydrophilic co-monomer, were prepared for the first time. RAM-MIPs with hydrophilic external layer were characterized by scanning electron microscopy and recognition and selectivity properties were compared with the restricted access materials-non-molecularly imprinted polymers (RAM-NIPs) and unmodified MIPs. RAM-MIPs were used as the adsorbent enclosed in solid phase extraction column and several important extraction parameters were comprehensively optimized to evaluate the extraction performance. Under the optimum extraction conditions, RAM-MIPs exhibited comparable or even higher selectivity with greater extraction capacity toward six kinds of organophosphorus pesticides (including malathion, ethoprophos, phorate, terbufos, dimethoate, and fenamiphos) compared with the MIPs and commercial solid phase extraction columns. The RAM-MIPs solid phase extraction coupled with gas chromatography was successfully applied to simultaneously determine six kinds of organophosphorus pesticides from honey sample. The new established method showed good linearity in the range of 0.01-1.0 μg mL(-1), low limits of detection (0.0005-0.0019 μg mL(-1)), acceptable reproducibility (RSD, 2.26-4.81%, n = 6), and satisfactory relative recoveries (90.9-97.6%). It was demonstrated that RAM-MIPs solid phase extraction with excellent selectivity and restricted access function was a simple, rapid, selective, and effective sample pretreatment method.
authors.affiliation: School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou, China. Electronic address: juanhe@haut.edu.cn.
authors.forename: Juan
authors.initials: J
authors.lastname: He
authors.affiliation: Henan Vocational College of Waters Conservancy and Environment, Zhengzhou, China.
authors.forename: Lixin
authors.initials: L
authors.lastname: Song
authors.affiliation: School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou, China.
authors.forename: Si
authors.initials: S
authors.lastname: Chen
authors.affiliation: School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou, China.
authors.forename: Hongliang
authors.initials: H
authors.lastname: Wei
authors.affiliation: School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou, China.
authors.forename: Dongxin
authors.initials: D
authors.lastname: Zhao
authors.affiliation: School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou, China.
authors.forename: Keren
authors.initials: K
authors.lastname: Gu
authors.affiliation: Department of Chemistry, Zhengzhou University, Zhengzhou, China.
authors.forename: Shusheng
authors.initials: S
authors.lastname: Zhang
mesh_terms: D004117:Dimethoate / Q000032:analysis; D006722:Honey / Q000032:analysis*; D008294:Malathion / Q000032:analysis; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting / Q000379:methods*; D009943:Organophosphorus Compounds / Q000032:analysis*; D063086:Organothiophosphates; D009946:Organothiophosphorus Compounds / Q000032:analysis; D010575:Pesticides / Q000032:analysis*; D010702:Phorate / Q000032:analysis; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D009943:Organophosphorus Compounds; D063086:Organothiophosphates; D009946:Organothiophosphorus Compounds; D010575:Pesticides; D011108:Polymers; C001182:ethoprop; D010702:Phorate; C002698:fenamiphos; C012568:terbufos; D008294:Malathion; D004117:Dimethoate
keywords: Honey; Molecularly imprinted polymers; Organophosphorus pesticides; Restricted access materials