title: Characterization of a novel cyclase-like gene family involved in controlling stress tolerance in rice.
abstract: A novel cyclase-like gene family (CYL) encodes proteins containing cyclase domain, but their functions are largely unknown. We report the systematic identification and characterization of CYL genes in the rice genome. Five putative CYL protein sequences (OsCYL1 to 4b) were identified. These sequences and other CYL homologs were classified into four subgroups based on phylogenetic analysis. Distinct diversification of these CYL proteins exists between plants and non-plants. The CYL family has conserved exon-intron structures, and the organizations of putative motifs in plants are specifically diverse. All OsCYL genes were expressed in a wide range of tissues or organs and were responsive to at least one of the abiotic stresses and hormone treatments applied. Protein OsCYL4a is targeted to the cell membrane. The overexpression of one stress-responsive gene OsCYL4a in rice resulted in decreased tolerance to salt, drought, cold, and oxidative stress. The expression levels of some abiotic stress-responsive factors, including H2O2-accumulating negative factors DST and OsSKIPa in OsCYL4a-overexpressing plants, were reduced compared with the wild type under normal condition and drought stress. These results suggest that rice CYL family may be functionally conserved polyketide cyclase, resulting in the rapid accumulation of reactive oxygen species to decrease tolerance to abiotic stresses.
authors.affiliation: Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area, College of Life Sciences, South Central University for Nationalities, Wuhan 430074, China; National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Qin
authors.affiliation: Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area, College of Life Sciences, South Central University for Nationalities, Wuhan 430074, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Shen
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Nili
authors.initials: N
authors.lastname: Wang
authors.affiliation: Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture, Danzhou 571737, Hainan, China; National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China. Electronic address: xipding@163.com.
authors.forename: Xipeng
authors.initials: X
authors.lastname: Ding
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects / Q000235:genetics*; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects / Q000201:enzymology; D003080:Cold Temperature; D055864:Droughts; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family; D012275:Oryza / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D060888:Real-Time Polymerase Chain Reaction; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics*; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; D010937:Plant Growth Regulators; D010940:Plant Proteins
keywords: Abiotic stress; CYL family; Oryza sativa; OsCYL4a; Stress