title: Modified CTAB and TRIzol protocols improve RNA extraction from chemically complex Embryophyta.
abstract: OBJECTIVE
  Here we present a series of protocols for RNA extraction across a diverse array of plants; we focus on woody, aromatic, aquatic, and other chemically complex taxa.
  
  
  RESULTS
  Ninety-one taxa were subjected to RNA extraction with three methods presented here: (1) TRIzol/TURBO DNA-free kits using the manufacturer's protocol with the addition of sarkosyl; (2) a combination method using cetyltrimethylammonium bromide (CTAB) and TRIzol/sarkosyl/TURBO DNA-free; and (3) a combination of CTAB and QIAGEN RNeasy Plant Mini Kit. Bench-ready protocols are given.
  
  
  CONCLUSIONS
  After an iterative process of working with chemically complex taxa, we conclude that the use of TRIzol supplemented with sarkosyl and the TURBO DNA-free kit is an effective, efficient, and robust method for obtaining RNA from 100 mg of leaf tissue of land plant species (Embryophyta) examined. Our protocols can be used to provide RNA of suitable stability, quantity, and quality for transcriptome sequencing.
authors.affiliation: Department of Biology, University of Florida, Gainesville, Florida 32611 USA ; University and Jepson Herbaria, University of California Berkeley, Berkeley, California 94720 USA.
authors.forename: Ingrid E
authors.initials: IE
authors.lastname: Jordon-Thaden
authors.affiliation: Department of Biology, University of Florida, Gainesville, Florida 32611 USA.
authors.forename: Andre S
authors.initials: AS
authors.lastname: Chanderbali
authors.affiliation: Department of Biology, University of Florida, Gainesville, Florida 32611 USA.
authors.forename: Matthew A
authors.initials: MA
authors.lastname: Gitzendanner
authors.affiliation: Department of Biology, University of Florida, Gainesville, Florida 32611 USA ; Florida Museum of Natural History, University of Florida, Gainesville, Florida 32611 USA ; Genetics Institute, University of Florida, Gainesville, Florida 32611 USA.
authors.forename: Douglas E
authors.initials: DE
authors.lastname: Soltis
keywords: RNA; aquatic plants; aromatic plants; extraction methods; transcriptome; woody plants