title: CRISPR-Cas9-mediated genome editing and guide RNA design.
abstract: CRISPR and CRISPR-associated (Cas) proteins, which in nature comprise the RNA-based adaptive immune system in bacteria and archaea, have emerged as particularly powerful genome editing tools owing to their unrivaled ease of use and ability to modify genomes across mammalian model systems. As such, the CRISPR-Cas9 system holds promise as a "system of choice" for functional mammalian genetic studies across biological disciplines. Here we briefly review this fast moving field, introduce the CRISPR-Cas9 system and its application to genome editing, with a focus on the basic considerations in designing the targeting guide RNA sequence.
authors.affiliation: The Jackson Laboratory, 600 Main Street, Bar Harbor, ME, 04609-1500, USA.
authors.forename: Michael V
authors.initials: MV
authors.lastname: Wiles
authors.affiliation: The Jackson Laboratory, 600 Main Street, Bar Harbor, ME, 04609-1500, USA.
authors.forename: Wenning
authors.initials: W
authors.lastname: Qin
authors.affiliation: The Jackson Laboratory, 600 Main Street, Bar Harbor, ME, 04609-1500, USA.
authors.forename: Albert W
authors.initials: AW
authors.lastname: Cheng
authors.affiliation: The Jackson Laboratory, 600 Main Street, Bar Harbor, ME, 04609-1500, USA. haoyi.wang@jax.org.|State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, People's Republic of China. haoyi.wang@jax.org.
authors.forename: Haoyi
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D001483:Base Sequence / Q000235:genetics; D064113:CRISPR-Cas Systems / Q000235:genetics*; D005818:Genetic Engineering; D016678:Genome; D017393:RNA Editing / Q000235:genetics*; D000094704:RNA, Guide, CRISPR-Cas Systems / Q000235:genetics*
chemical_list: D000094704:RNA, Guide, CRISPR-Cas Systems