title: Detection of Potato spindle tuber viroid and Other Related Viroids by a DIG Labelled RNA Probe.
abstract: Viroids can cause diseases of considerable economic importance; in Europe the main concern is with pospiviroids that may affect the tomato and potato industries. Methods for detection are required that are both sensitive and robust. The detection method described here is a probe hybridization method with a commercially available digoxigenin (DIG) labelled full-length Potato spindle tuber viroid (PSTVd) RNA probe. This method detects PSTVd and all other known pospiviroids.
authors.affiliation: Science and Advice for Scottish Agriculture, Roddinglaw Road, Edinburgh, EH12 9FJ, UK, wendy.monger@sasa.gsi.gov.uk.
authors.forename: Wendy A
authors.initials: WA
authors.lastname: Monger
authors.forename: Colin
authors.initials: C
authors.lastname: Jeffries
mesh_terms: D004076:Digoxigenin / Q000737:chemistry*; D010935:Plant Diseases / Q000382:microbiology*; D035281:Plant Tubers / Q000821:virology*; D015347:RNA Probes / Q000737:chemistry*; D012367:RNA, Viral / Q000032:analysis* / Q000235:genetics; D011198:Solanum tuberosum / Q000821:virology*; D013045:Species Specificity; D014772:Viroids / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D015347:RNA Probes; D012367:RNA, Viral; D004076:Digoxigenin