title: Use of tilapia piscidin 3 (TP3) to protect against MRSA infection in mice with skin injuries.
abstract: Antimicrobial peptides (AMPs), represent promising agents for new therapeutic approaches of infected wound treatment, on account of their antimicrobial and wound closure activities, and low potential for inducing resistance. However, therapeutic applications of these AMPs are limited by their toxicity and low stability in vivo. Previously, we reported that the 23 amino-acid designer peptide TP3 possessed antimicrobial activities. Here, we analyzed the wound closure activities of TP3 both and in vivo. TP3 at doses of up to 40 μg/ml did not affect the viability of baby hamster kidney cells. Furthermore, TP3 was found to be highly effective at combating peritonitis and wound infection caused by MRSA in mouse models, without inducing adverse behavioral effects or liver or kidney toxicity. TP3 treatment increased survival by 100% at 8 days after infection, and accelerated the progression of proliferation, remodeling, and maturation of infected wounds. Taken together, our results indicate that TP3 enhances the rate of survival of mice infected with the bacterial pathogen MRSA through both antimicrobial and immunomodulatory effects. Overall, these results suggest that TP3 may be suitable for development as a novel topical agent for treatment of infected wounds.
authors.affiliation: Marine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica, Jiaushi, Ilan, Taiwan.
authors.forename: Han-Ning
authors.initials: HN
authors.lastname: Huang
authors.affiliation: Department of Life Science, Chinese Culture University, Taipei, Taiwan.
authors.forename: Yi-Lin
authors.initials: YL
authors.lastname: Chan
authors.affiliation: Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan.
authors.forename: Cho-Fat
authors.initials: CF
authors.lastname: Hui
authors.affiliation: Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan.
authors.forename: Jen-Leih
authors.initials: JL
authors.lastname: Wu
authors.affiliation: Department of Food Science, National Taiwan Ocean University, Keelung, Taiwan.
authors.forename: Chang-Jer
authors.initials: CJ
authors.lastname: Wu
authors.affiliation: Marine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica, Jiaushi, Ilan, Taiwan.
authors.forename: Jyh-Yih
authors.initials: JY
authors.lastname: Chen
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D016470:Bacteremia / Q000188:drug therapy / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control; D006224:Cricetinae; D004357:Drug Synergism; D005260:Female; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000494:pharmacology*; D012867:Skin / Q000293:injuries / Q000382:microbiology; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000276:immunology / Q000517:prevention & control*; D013207:Staphylococcal Skin Infections / Q000188:drug therapy / Q000276:immunology / Q000517:prevention & control; D013946:Thymopoietins / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects* / Q000276:immunology; D014946:Wound Infection / Q000188:drug therapy / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*
chemical_list: D000890:Anti-Infective Agents; D010446:Peptide Fragments; D013946:Thymopoietins; C051920:splenotritin
keywords: Pathology Section; antimicrobial peptides; staphylococcus aureus; tilapia piscidin 3; wound healing