title: Determination of malachite green in fish based on magnetic molecularly imprinted polymer extraction followed by electrochemiluminescence.
abstract: A novel procedure for selective extraction of malachite green (MG) from fish samples was set up by using magnetic molecularly imprinted polymers (MMIP) as the solid phase extraction material followed by electrochemiluminescence (ECL) determination. MMIP was prepared by using Fe3O4 magnetite as magnetic component, MG as template molecule, methacrylic acid (MAA) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as crosslinking agent. MMIP was characterized by SEM, TEM, FT-IR, VSM and XRD. Leucomalachite green (LMG) was oxidized in situ to MG by 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ). And then MMIP was successfully used to selectively enrich MG from fish samples. Adsorbed MG was desorbed and determined by ECL. Under the optimal conditions, calibration curve was good linear in the range of 0.29-290 μg/kg and the limit of detection (LOD) was 7.3 ng/kg (S/N=3). The recoveries of MMIP extraction were 77.1-101.2%. In addition, MMIP could be regenerated. To the best of our knowledge, MMIP coupling with ECL quenching of Ru(bpy)3(2+)/TPA for the determination of MG has not yet been developed.
authors.affiliation: College of Chemistry & Chemical Engineering, MianYang Normal University, MianYang 621000, China; Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou 730070, China.
authors.forename: Baomei
authors.initials: B
authors.lastname: Huang
authors.affiliation: Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou 730070, China.
authors.forename: Xibin
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou 730070, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.affiliation: Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou 730070, China.
authors.forename: Guofan
authors.initials: G
authors.lastname: Wu
authors.affiliation: Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou 730070, China. Electronic address: luxq@nwnu.edu.cn.
authors.forename: Xiaoquan
authors.initials: X
authors.lastname: Lu
mesh_terms: D000327:Adsorption; D000818:Animals; D000891:Anti-Infective Agents, Local / Q000032:analysis / Q000737:chemistry; D004396:Coloring Agents / Q000032:analysis / Q000737:chemistry; D005399:Fishes; D005506:Food Contamination / Q000032:analysis*; D005659:Fungicides, Industrial / Q000032:analysis / Q000737:chemistry; D060328:Magnetic Phenomena; D058185:Magnetite Nanoparticles / Q000737:chemistry; D054802:Molecular Imprinting; D009942:Organometallic Compounds / Q000737:chemistry; D011437:Propylamines / Q000737:chemistry; D012394:Rosaniline Dyes / Q000032:analysis* / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000737:chemistry
chemical_list: D000891:Anti-Infective Agents, Local; D004396:Coloring Agents; D005659:Fungicides, Industrial; D058185:Magnetite Nanoparticles; D009942:Organometallic Compounds; D011437:Propylamines; D012394:Rosaniline Dyes; D014874:Water Pollutants, Chemical; C415768:tris(2,2'-bipyridyl)ruthenium(II); C096226:tripropylamine; C005095:malachite green; D012822:Silicon Dioxide
keywords: Electrochemiluminescence; Fish; Magnetic molecularly imprinted polymers; Malachite green; Quenching