title: Development of a microparticulate prostate cancer vaccine and evaluating the effect of route of administration on its efficacy via the skin.
abstract: The skin has been identified as a promising target to deliver vaccines. In this study, prostate cancer antigens were delivered in a spray-dried microparticulate carrier to a murine model via the transdermal route and the subcutaneous route. There was a significant increase in the humoral responses as determined by the total serum IgG titres (p < 0.05) and the cellular responses as determined by the T- and B-cells sub-population in spleen samples and delay in tumour growth till 8 weeks post-tumour challenge of both vaccinated groups when compared to the controls. The vaccine microparticles administered via the transdermal route induced a Th2-mediated immune response versus a mixed Th1- and Th2-mediated immune response via the subcutaneous route. Thus, the particulate vaccine delivery system proves to be a promising alternative for generation of a robust immune response against prostate cancer via the skin in a murine model.
authors.affiliation: Department of Bioengineering, University of Louisville , Louisville, KY , USA .
authors.forename: Archana
authors.initials: A
authors.lastname: Akalkotkar
authors.forename: Lipika
authors.initials: L
authors.lastname: Chablani
authors.forename: Suprita A
authors.initials: SA
authors.lastname: Tawde
authors.forename: Cherilyn
authors.initials: C
authors.lastname: D'Souza
authors.forename: Martin J
authors.initials: MJ
authors.lastname: D'Souza
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000473:pathology; D019496:Cancer Vaccines / Q000008:administration & dosage* / Q000276:immunology / Q000627:therapeutic use*; D002460:Cell Line; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems; D006801:Humans; D007114:Immunization; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011467:Prostate / Q000276:immunology / Q000473:pathology*; D011471:Prostatic Neoplasms / Q000097:blood / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology
chemical_list: D019496:Cancer Vaccines; D007074:Immunoglobulin G
keywords: Microneedles; TRAMP C2; prostate cancer vaccine; spray drying; transdermal; whole cell lysate