title: Digesting pythons quickly oxidize the proteins in their meals and save the lipids for later.
abstract: Pythons digesting rodent meals exhibit up to 10-fold increases in their resting metabolic rate (RMR); this increase in RMR is termed specific dynamic action (SDA). Studies have shown that SDA is partially fueled by oxidizing dietary nutrients, yet it remains unclear whether the proteins and the lipids in their meals contribute equally to this energy demand. We raised two populations of mice on diets labeled with either [(13)C]leucine or [(13)C]palmitic acid to intrinsically enrich the proteins and lipids in their bodies, respectively. Ball pythons (Python regius) were fed whole mice (and pureed mice 3 weeks later), after which we measured their metabolic rates and the δ(13)C in the breath. The δ(13)C values in the whole bodies of the protein- and lipid-labeled mice were generally similar (i.e. 5.7±4.7‰ and 2.8±5.4‰, respectively) but the oxidative kinetics of these two macronutrient pools were quite different. We found that the snakes oxidized 5% of the protein and only 0.24% of the lipids in their meals within 14 days. Oxidation of the dietary proteins peaked 24 h after ingestion, at which point these proteins provided ∼90% of the metabolic requirement of the snakes, and by 14 days the oxidation of these proteins decreased to nearly zero. The oxidation of the dietary lipids peaked 1 day later, at which point these lipids supplied ∼25% of the energy demand. Fourteen days after ingestion, these lipids were still being oxidized and continued to account for ∼25% of the metabolic rate. Pureeing the mice reduced the cost of gastric digestion and decreased SDA by 24%. Pureeing also reduced the oxidation of dietary proteins by 43%, but it had no effect on the rates of dietary lipid oxidation. Collectively, these results demonstrate that pythons are able to effectively partition the two primary metabolic fuels in their meals. This approach of uniquely labeling the different components of the diet will allow researchers to examine new questions about how and when animals use the nutrients in their meals.
authors.affiliation: Department of Biological Sciences, St Mary's University, San Antonio, TX 78228, USA mmccue1@stmarytx.edu.
authors.forename: Marshall D
authors.initials: MD
authors.lastname: McCue
authors.affiliation: Department of Biological Sciences, St Mary's University, San Antonio, TX 78228, USA.
authors.forename: R Marena
authors.initials: RM
authors.lastname: Guzman
authors.affiliation: Department of Biological Sciences, St Mary's University, San Antonio, TX 78228, USA.
authors.forename: Celeste A
authors.initials: CA
authors.lastname: Passement
mesh_terms: D000818:Animals; D001481:Basal Metabolism; D017821:Boidae / Q000378:metabolism*; D004041:Dietary Fats / Q000378:metabolism; D004044:Dietary Proteins / Q000378:metabolism*; D004063:Digestion / Q000502:physiology*; D051379:Mice; D010084:Oxidation-Reduction; D019518:Postprandial Period / Q000502:physiology
chemical_list: D004041:Dietary Fats; D004044:Dietary Proteins
keywords: Metabolism; Nutrition; Snakes; Specific dynamic action; Stable isotopes