title: Circulating inflammatory miRNA signature in response to different doses of aerobic exercise.
abstract: While moderate acute exercise has been associated with strong anti-inflammatory mechanisms, strenuous exercise has been linked to deleterious inflammatory perturbations. It is therefore fundamental to elucidate the mechanisms that regulate the exercise-induced inflammatory cascade. Information on novel regulators such as circulating inflammatory microRNAs (c-inflammamiRs) is incomplete. In this study, we evaluated the response of a panel of c-inflammamiRs to different doses of acute aerobic exercise. We first studied the exercise-induced inflammatory cascade in serum samples of nine active middle-aged males immediately before and after (0 h, 24 h, 72 h) 10-km, half-marathon, and marathon races. Next, we analyzed the circulating profile of 106 specific c-inflammamiRs immediately before) and after (0 h, 24 h) 10-km (low inflammatory response) and marathon (high inflammatory response) races. Analysis of classical inflammatory parameters revealed a dose-dependent effect of aerobic exercise on systemic inflammation, with higher levels detected after marathon. We observed an increase in miR-150-5p immediately after the 10-km race. Levels of 12 c-inflammamiRs were increased immediately after the marathon (let-7d-3p, let-7f-2-3p, miR-125b-5p, miR-132-3p, miR-143-3p, miR-148a-3p, miR-223-3p, miR-223-5p, miR-29a-3p, miR-34a-5p, miR-424-3p, and miR-424-5p). c-inflammamiRs returned to basal levels after 24 h. Correlation and in silico analyses supported a close association between the observed c-inflammamiR pattern and regulation of the inflammatory process. In conclusion, we found that different doses of acute aerobic exercise induced a distinct and specific c-inflammamiR response, which may be associated with control of the exercise-induced inflammatory cascade. Our findings point to c-inflammamiRs as potential biomarkers of exercise-induced inflammation, and hence, exercise dose.
authors.affiliation: Lipids and Cardiovascular Pathology Group, Research Institute of the Sant Pau Hospital (IIB Sant Pau), Barcelona, Spain; Department of Functional Biology (Physiology), University of Oviedo, Oviedo, Spain; david.degonzalo@gmail.com.
authors.forename: David
authors.initials: D
authors.lastname: de Gonzalo-Calvo
authors.affiliation: Laboratory of Disorders of Lipid Metabolism and Molecular Nutrition, Madrid Institute for Advanced Studies-Food, Campus de Excelencia Internacional Autonomous University of Madrid and Spanish National Research Council, Madrid, Spain;
authors.forename: Alberto
authors.initials: A
authors.lastname: Dávalos
authors.affiliation: Department of Pharmaceutical and Health Sciences, Centro de Estudios Universitarios San Pablo University, Madrid, Spain;
authors.forename: Ana
authors.initials: A
authors.lastname: Montero
authors.affiliation: Department of Pharmaceutical and Health Sciences, Centro de Estudios Universitarios San Pablo University, Madrid, Spain;
authors.forename: Ángela
authors.initials: Á
authors.lastname: García-González
authors.affiliation: Laboratory of Disorders of Lipid Metabolism and Molecular Nutrition, Madrid Institute for Advanced Studies-Food, Campus de Excelencia Internacional Autonomous University of Madrid and Spanish National Research Council, Madrid, Spain;
authors.forename: Iryna
authors.initials: I
authors.lastname: Tyshkovska
authors.affiliation: Department of Pharmaceutical and Health Sciences, Centro de Estudios Universitarios San Pablo University, Madrid, Spain;
authors.forename: Antonio
authors.initials: A
authors.lastname: González-Medina
authors.affiliation: Laboratory of Disorders of Lipid Metabolism and Molecular Nutrition, Madrid Institute for Advanced Studies-Food, Campus de Excelencia Internacional Autonomous University of Madrid and Spanish National Research Council, Madrid, Spain;
authors.forename: Sara M A
authors.initials: SM
authors.lastname: Soares
authors.affiliation: Hospital Universitario Central de Asturias, Hospital Univeritario Central de Asturias, Asturias, Spain; Universidad Autónoma de Chile, Santiago, Chile; and.
authors.forename: Pablo
authors.initials: P
authors.lastname: Martínez-Camblor
authors.affiliation: Laboratory of Disorders of Lipid Metabolism and Molecular Nutrition, Madrid Institute for Advanced Studies-Food, Campus de Excelencia Internacional Autonomous University of Madrid and Spanish National Research Council, Madrid, Spain;
authors.forename: Patricia
authors.initials: P
authors.lastname: Casas-Agustench
authors.affiliation: Sports Medicine Center, Clinical Laboratory, Higher Council for Sports, Madrid, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Rabadán
authors.affiliation: Sports Medicine Center, Clinical Laboratory, Higher Council for Sports, Madrid, Spain.
authors.forename: Ángel E
authors.initials: ÁE
authors.lastname: Díaz-Martínez
authors.affiliation: Department of Pharmaceutical and Health Sciences, Centro de Estudios Universitarios San Pablo University, Madrid, Spain;
authors.forename: Natalia
authors.initials: N
authors.lastname: Úbeda
authors.affiliation: Department of Functional Biology (Physiology), University of Oviedo, Oviedo, Spain; Department of Pharmaceutical and Health Sciences, Centro de Estudios Universitarios San Pablo University, Madrid, Spain;
authors.forename: Eduardo
authors.initials: E
authors.lastname: Iglesias-Gutiérrez
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D015444:Exercise / Q000502:physiology*; D006801:Humans; D007249:Inflammation / Q000097:blood*; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D012420:Running / Q000502:physiology
chemical_list: D015415:Biomarkers; D035683:MicroRNAs
keywords: circulating microRNAs; exercise; inflammation