title: Electrochemiluminescent DNA sensor based on controlled Zn-mediated grafting of diazonium precursors.
abstract: Controlled Zn-mediated grafting of a thin layer of a diazonium salt was used to functionalise a carbon electrode with ruthenium(II)-tris-bipyridine (Ru)-labelled DNA for use as a capture probe in an electrochemiluminescent genosensor. A secondary reporter probe was labelled with a ferrocene (Fc) molecule, and in the presence of the single-stranded DNA target a genocomplex formed, where the Fc-label effectively quenched the electrochemiluminescence of the signal emitted from the Ru-label. The spacing of the labels for maximum sensitivity and minimum detection limit was optimised, and the signal reproducibility and stability of the method was established.
authors.affiliation: Departament d'Enginyeria Química, Universitat Rovira i Virgili, Avinguda Països Catalans, 26, 43007, Tarragona, Spain.
authors.forename: Mabel
authors.initials: M
authors.lastname: Torréns
authors.forename: Mayreli
authors.initials: M
authors.lastname: Ortiz
authors.forename: Diego
authors.initials: D
authors.lastname: Bejarano-Nosas
authors.forename: Ciara K
authors.initials: CK
authors.lastname: O'Sullivan
mesh_terms: D004247:DNA / Q000032:analysis*; D003979:Diazonium Compounds / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000295:instrumentation*; D057230:Limit of Detection; D049449:Luminescence; D015203:Reproducibility of Results; D015032:Zinc / Q000737:chemistry*
chemical_list: D003979:Diazonium Compounds; D004247:DNA; D015032:Zinc