title: Oxaliplatin enhances gap junction-mediated coupling in cell cultures of mouse trigeminal ganglia.
abstract: Communications between satellite glial cells and neighboring neurons within sensory ganglia may contribute to neuropathic and inflammatory pain. To elucidate the role of satellite glial cells in chemotherapy-induced pain, we examined the effects of oxaliplatin on the gap junction-mediated coupling between these cells. We also examined whether the gap junction blocker, carbenoxolone, can reverse the coupling. Primary cultures of mice trigeminal ganglia, 24-48h after cell isolation, were used. Satellite glial cells were injected with Lucifer yellow in the presence or absence of oxaliplatin (60 μM). In addition, the effect of carbenoxolone (100 μM) on coupling, and the expression of connexin 43 proteins were evaluated. Dye coupling between adjacent satellite glial cells was significantly increased (2.3-fold, P<0.05) following a 2h incubation with oxaliplatin. Adding carbenoxolone to the oxaliplatin-treated cultures reversed oxaliplatin-evoked coupling to baseline (P<0.05). Immunostaining showed no difference between expression of connexin 43 in control and oxaliplatin-treated cultures. Our findings indicated that oxaliplatin-increased gap junction-mediated coupling between satellite glial cells in primary cultures of mouse trigeminal ganglia, and carbenoxolone reversed this effect. Hence, it is proposed that increased gap junction-mediated coupling was seen between satellite glial cells in TG. This observation together with our previous data obtained from a behavioral study suggests that this phenomenon might contribute to chemotherapy-induced nociception following oxaliplatin treatment.
authors.affiliation: Center for Sensory-Motor Interaction, Department of Health Science and Technology, Faculty of Medicine, Aalborg University, Fredrik Bajers Vej 7D3, Aalborg East DK-9220, Denmark.
authors.forename: Jeppe Nørgaard
authors.initials: JN
authors.lastname: Poulsen
authors.affiliation: Laboratory of Experimental Surgery, Hadassah University Hospital, Mount Scopus, Jerusalem, Israel.
authors.forename: Rebekah
authors.initials: R
authors.lastname: Warwick
authors.affiliation: Laboratory for Cancer Biology, Biomedicine, Department of Health Science and Technology, Faculty of Medicine, Aalborg University, Fredrik Bajers Vej 3B, Aalborg East DK-9220, Denmark.
authors.forename: Meg
authors.initials: M
authors.lastname: Duroux
authors.affiliation: Laboratory of Experimental Surgery, Hadassah University Hospital, Mount Scopus, Jerusalem, Israel.
authors.forename: Menachem
authors.initials: M
authors.lastname: Hanani
authors.affiliation: Center for Sensory-Motor Interaction, Department of Health Science and Technology, Faculty of Medicine, Aalborg University, Fredrik Bajers Vej 7D3, Aalborg East DK-9220, Denmark; Laboratory for Cancer Biology, Biomedicine, Department of Health Science and Technology, Faculty of Medicine, Aalborg University, Fredrik Bajers Vej 3B, Aalborg East DK-9220, Denmark. Electronic address: gazerani@hst.aau.dk.
authors.forename: Parisa
authors.initials: P
authors.lastname: Gazerani
mesh_terms: D000818:Animals; D000897:Anti-Ulcer Agents; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002229:Carbenoxolone; D002478:Cells, Cultured; D018031:Connexin 43 / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D005455:Fluorescent Antibody Technique; D017629:Gap Junctions / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009457:Neuroglia / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009944:Organoplatinum Compounds / Q000494:pharmacology*; D000077150:Oxaliplatin; D027161:Satellite Cells, Perineuronal / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D012668:Trigeminal Ganglion / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
chemical_list: D000897:Anti-Ulcer Agents; D000970:Antineoplastic Agents; D018031:Connexin 43; D009944:Organoplatinum Compounds; D000077150:Oxaliplatin; D002229:Carbenoxolone
keywords: Carbenoxolone; Chemotherapy-induced pain; Connexin 43; Oxaliplatin; Satellite glial cells; Trigeminal ganglion