title: A Gibberellin-Mediated DELLA-NAC Signaling Cascade Regulates Cellulose Synthesis in Rice.
abstract: Cellulose, which can be converted into numerous industrial products, has important impacts on the global economy. It has long been known that cellulose synthesis in plants is tightly regulated by various phytohormones. However, the underlying mechanism of cellulose synthesis regulation remains elusive. Here, we show that in rice (Oryza sativa), gibberellin (GA) signals promote cellulose synthesis by relieving the interaction between SLENDER RICE1 (SLR1), a DELLA repressor of GA signaling, and NACs, the top-layer transcription factors for secondary wall formation. Mutations in GA-related genes and physiological treatments altered the transcription of CELLULOSE SYNTHASE genes (CESAs) and the cellulose level. Multiple experiments demonstrated that transcription factors NAC29/31 and MYB61 are CESA regulators in rice; NAC29/31 directly regulates MYB61, which in turn activates CESA expression. This hierarchical regulation pathway is blocked by SLR1-NAC29/31 interactions. Based on the results of anatomical analysis and GA content examination in developing rice internodes, this signaling cascade was found to be modulated by varied endogenous GA levels and to be required for internode development. Genetic and gene expression analyses were further performed in Arabidopsis thaliana GA-related mutants. Altogether, our findings reveal a conserved mechanism by which GA regulates secondary wall cellulose synthesis in land plants and provide a strategy for manipulating cellulose production and plant growth.
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Debao
authors.initials: D
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Shaogan
authors.initials: S
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Baocai
authors.identifier: http://orcid.org/0000-0002-3239-7263
authors.initials: B
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Keke
authors.initials: K
authors.lastname: Shang-Guan
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Yanyun
authors.initials: Y
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Dongmei
authors.identifier: http://orcid.org/0000-0003-4560-9058
authors.initials: D
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Xiangling
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Kun
authors.identifier: http://orcid.org/0000-0003-1998-7987
authors.initials: K
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Zuopeng
authors.initials: Z
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Xiangdong
authors.identifier: http://orcid.org/0000-0001-9285-7543
authors.initials: X
authors.lastname: Fu
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China yhzhou@genetics.ac.cn.
authors.forename: Yihua
authors.identifier: http://orcid.org/0000-0001-6644-610X
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D002482:Cellulose / Q000096:biosynthesis*; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D017343:Genes, Plant / Q000502:physiology*; D005875:Gibberellins / Q000502:physiology*; D005964:Glucosyltransferases / Q000235:genetics / Q000502:physiology; D012275:Oryza / Q000378:metabolism / Q000502:physiology*; D010937:Plant Growth Regulators / Q000502:physiology*; D010940:Plant Proteins / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D005875:Gibberellins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D002482:Cellulose; D005964:Glucosyltransferases; C478648:cellulose synthase