title: PROTEOMIC ANALYSIS OF UBIQUITINATED PROTEINS FROM DELTAMETHRIN-RESISTANT AND SUSCEPTIBLE STRAINS OF THE DIAMONDBACK MOTH, Plutella Xylostella L.
abstract: Ubiquitin, a small protein consisting of 76 amino acids, acts in protein degradation, DNA repair, signal transduction, transcriptional regulation, and receptor control through endocytosis. Using proteomics, we compared the differentially ubiquitinated proteins between a deltamethrin-resistant (DR) strain and a deltamethrin-sensitive (DS) strain in third-instar larvae of the diamondback moth. We used polyubiquitin affinity beads to enrich ubiquitinated proteins and then performed one-dimensional SDS-PAGE separation and mass spectrometric identification. In the DR strain, We found 17 proteins that were upregulated (relative to the DS strain), including carbonic anhydrase family members, ADP ribosylation factor 102F CG11027-PA, protein kinase 61C, phospholipase A2 , dihydrolipoamide dehydrogenase, tyrosine hydroxylase, and heat shock proteins, and five proteins that were downregulated in the DS strain, including carboxylesterase and DNA cytosine-5 methyltransferase. These results were also verified by qPCR. The differentially ubiquitinated proteins/enzymes were mainly responsible for protein binding, catalytic activity, and molecular transducer activity. These results improve our understanding of the relationship between protein ubiquitination and the deltamethrin stress response.
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, School of Life Science, Nanjing Normal University, Nanjing, PR China.
authors.forename: Luogen
authors.initials: L
authors.lastname: Cheng
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, School of Life Science, Nanjing Normal University, Nanjing, PR China.
authors.forename: Yaqiong
authors.initials: Y
authors.lastname: Du
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, School of Life Science, Nanjing Normal University, Nanjing, PR China.
authors.forename: Junli
authors.initials: J
authors.lastname: Hu
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, School of Life Science, Nanjing Normal University, Nanjing, PR China.
authors.forename: Dongxu
authors.initials: D
authors.lastname: Jiao
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, School of Life Science, Nanjing Normal University, Nanjing, PR China.
authors.forename: Jin
authors.initials: J
authors.lastname: Li
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, School of Life Science, Nanjing Normal University, Nanjing, PR China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: School of Energy and Mechanical Engineering, Nanjing Normal University, Nanjing, PR China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Institute of Plant Protection, Guizhou Academy of Agriculture Science, Guiyang, PR China.
authors.forename: Fengliang
authors.initials: F
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019476:Insect Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D007305:Insecticide Resistance; D007814:Larva / Q000187:drug effects / Q000378:metabolism; D008969:Molecular Sequence Data; D009036:Moths / Q000187:drug effects* / Q000378:metabolism*; D009570:Nitriles / Q000494:pharmacology*; D040901:Proteomics / Q000379:methods; D011722:Pyrethrins / Q000494:pharmacology*; D057149:Ubiquitinated Proteins
chemical_list: D019476:Insect Proteins; D009570:Nitriles; D011722:Pyrethrins; D057149:Ubiquitinated Proteins; C017180:decamethrin
keywords: LC MS/MS; Plutella xylostella; deltamethrin; stress; ubiquitinated protein