title: [Detection of p16 by fluorescence in-situ hybridization and immunohistochemistry in malignant mesothelioma].
abstract: OBJECTIVE
  To study the role of p16 gene mutation status as detected by fluorescence in-situ hybridization (FISH) and p16 protein expression as detected by immunohistochemistry in differential diagnosis of malignant mesothelioma and benign mesothelial hyperplasia.
  
  
  METHODS
  p16 gene mutation status and protein expression were detected by FISH and immunohistochemistry respectively in 55 cases of pleural malignant mesothelioma and 30 cases of benign mesothelial hyperplasia.
  
  
  RESULTS
  FISH study showed that the rate of p16 deletion in malignant mesothelioma (81.8%,45/55) was higher than that in benign mesothelial hyperplasia (3.3%,1/30). The difference was statistically significant (P<0.05). Immunohistochemical study showed that the rate of p16 protein expression in malignant mesothelioma (23.6%) was lower than that in benign mesothelial hyperplasia (76.7%). The difference was also statistically significant. The sensitivity and specificity of FISH in distinguishing between mesothelioma and reactive mesothelial hyperplasia were higher than those of immunohistochemistry.
  
  
  CONCLUSIONS
  In contrast to reactive mesothelial hyperplasia, p16 gene is deleted and p16 protein is not expressed in malignant mesothelioma. The sensitivity and specificity of FISH are higher than those of immunohistochemistry in the distinction.
authors.affiliation: Department of Pathology, Shenyang Chest Hospital, Shenyang 110044, China; E-mail: xkly13@126.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Pathology, Shenyang Chest Hospital, Shenyang 110044, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Pathology, Shenyang Chest Hospital, Shenyang 110044, China.
authors.forename: Ningning
authors.initials: N
authors.lastname: Ding
authors.affiliation: Department of Pathology, Shenyang Chest Hospital, Shenyang 110044, China.
authors.forename: Wenxiang
authors.initials: W
authors.lastname: Xu
authors.affiliation: Department of Pathology, Shenyang Chest Hospital, Shenyang 110044, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Pathology, Shenyang Chest Hospital, Shenyang 110044, China.
authors.forename: Xuechen
authors.initials: X
authors.lastname: Chen
mesh_terms: D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000378:metabolism*; D003937:Diagnosis, Differential; D004848:Epithelium / Q000473:pathology; D019942:Genes, p16; D006801:Humans; D006965:Hyperplasia / Q000175:diagnosis / Q000235:genetics; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D008654:Mesothelioma / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D010994:Pleura / Q000473:pathology*; D010997:Pleural Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D012680:Sensitivity and Specificity
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16