title: Analysis of Serum MicroRNAs as Potential Biomarker in Coronary Bifurcation Lesion.
abstract: Recent evidence suggests that cell-derived circulating miRNAs may serve as the biomarkers of cardiovascular diseases. However, no study has investigated the potential of circulating miRNAs as biomarker for coronary bifurcation lesion. In this study, we aimed to characterize the miRNA profiles that could distinguish coronary bifurcation lesion and identify potential miRNAs as biomarkers of coronary bifurcation lesion. We employed miRNA microarray to screen serum miRNAs profiles of patients with coronary bifurcation lesion and coronary nonbifurcation lesions. We identified 197 miRNAs differentially expressed, including 150 miRNAs upregulated and 47 miRNAs downregulated. We chose 3 miRNAs with significant differences for further testing in 200 patients. RT-PCR analysis of serum samples confirmed that miR30d was upregulated and miR1246 was downregulated in the serum of coronary bifurcation lesion patients compared with nonbifurcation lesion patients. Our findings suggest that these miRNAs may have a role in the pathogenesis of coronary bifurcation lesion and may represent novel biomarkers for the diagnosis and prognosis of coronary bifurcation lesion.
authors.affiliation: Department of Cardiology, Nanjing First Hospital, Nanjing Medical University, Changle Road No. 68, Nanjing, Jiangsu 210029, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Cardiology, Nanjing First Hospital, Nanjing Medical University, Changle Road No. 68, Nanjing, Jiangsu 210029, China.
authors.forename: Shaoliang
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Cardiology, Nanjing First Hospital, Nanjing Medical University, Changle Road No. 68, Nanjing, Jiangsu 210029, China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, Nanjing First Hospital, Nanjing Medical University, Changle Road No. 68, Nanjing, Jiangsu 210029, China.
authors.forename: Shoujie
authors.initials: S
authors.lastname: Shan
authors.affiliation: Department of Cardiology, Nanjing First Hospital, Nanjing Medical University, Changle Road No. 68, Nanjing, Jiangsu 210029, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Cardiology, Nanjing First Hospital, Nanjing Medical University, Changle Road No. 68, Nanjing, Jiangsu 210029, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Nanjing First Hospital, Nanjing Medical University, Changle Road No. 68, Nanjing, Jiangsu 210029, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Kan
authors.affiliation: Department of Cardiology, Nanjing First Hospital, Nanjing Medical University, Changle Road No. 68, Nanjing, Jiangsu 210029, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Xu
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D003324:Coronary Artery Disease / Q000097:blood* / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged
chemical_list: D015415:Biomarkers; D035683:MicroRNAs