title: A continuous tyrosyl-tRNA synthetase assay that regenerates the tRNA substrate.
abstract: Tyrosyl-tRNA synthetase catalyzes the attachment of tyrosine to the 3' end of tRNA(Tyr), releasing AMP, pyrophosphate, and l-tyrosyl-tRNA as products. Because this enzyme plays a central role in protein synthesis, it has garnered attention as a potential target for the development of novel antimicrobial agents. Although high-throughput assays that monitor tyrosyl-tRNA synthetase activity have been described, these assays generally use stoichiometric amounts of tRNA, limiting their sensitivity and increasing their cost. Here, we describe an alternate approach in which the Tyr-tRNA product is cleaved, regenerating the free tRNA substrate. We show that cyclodityrosine synthase from Mycobacterium tuberculosis can be used to cleave the l-Tyr-tRNA product, regenerating the tRNA(Tyr) substrate. Because tyrosyl-tRNA synthetase can use both l- and d-tyrosine as substrates, we replaced the cyclodityrosine synthase in the assay with d-tyrosyl-tRNA deacylase, which cleaves d-Tyr-tRNA. This substitution allowed us to use the tyrosyl-tRNA synthetase assay to monitor the aminoacylation of tRNA(Tyr) by d-tyrosine. Furthermore, by making Tyr-tRNA cleavage the rate-limiting step, we are able to use the assay to monitor the activities of cyclodityrosine synthetase and d-tyrosyl-tRNA deacylase. Specific methods to extend the tyrosyl-tRNA synthetase assay to monitor both the aminoacylation and post-transfer editing activities in other aminoacyl-tRNA synthetases are discussed.
authors.affiliation: Department of Biochemistry and Molecular Biology, Louisiana State University Health Sciences Center in Shreveport, Shreveport, LA 71130, USA.
authors.forename: Charles J
authors.initials: CJ
authors.lastname: Richardson
authors.affiliation: Department of Biochemistry and Molecular Biology, Louisiana State University Health Sciences Center in Shreveport, Shreveport, LA 71130, USA. Electronic address: efirst@lsuhsc.edu.
authors.forename: Eric A
authors.initials: EA
authors.lastname: First
mesh_terms: D000249:Adenosine Monophosphate / Q000378:metabolism; D049148:Aminoacylation; D057075:Enzyme Assays / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000201:enzymology; D017393:RNA Editing; D012343:RNA, Transfer / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism; D014448:Tyrosine-tRNA Ligase / Q000378:metabolism*
chemical_list: D000249:Adenosine Monophosphate; D014443:Tyrosine; D012343:RNA, Transfer; D014448:Tyrosine-tRNA Ligase
keywords: Cyclodityrosine synthase; High-throughput assay; Tyrosyl-tRNA synthetase; d-Tyrosyl-tRNA deacylase