title: Glycoprotein labeling with click chemistry (GLCC) and carbohydrate detection.
abstract: Molecular labeling and detection techniques are essential to research in life science. Here, a method for glycoprotein labeling/carbohydrate detection through glycan replacement, termed glycoprotein labeling with click chemistry (GLCC), is described. In this method, a glycoprotein is first treated with specific glycosidases to remove certain sugar residues, a procedure that creates acceptor sites for a specific glycosyltransferase. A 'clickable' monosaccharide is then installed onto these sites by the glycosyltransferase. This modified glycoprotein is then conjugated to a reporter molecule using a click chemistry reaction. For glycoproteins that already contain vacant glycosylation sites, deglycosylation is not needed before the labeling step. As a demonstration, labeling on fetal bovine fetuin, mouse immunoglobulin IgG and bacterial expressed human TNFα and TNFβ are shown. Compared to traditional ways of protein labeling, labeling at glycosylation sites with GLCC is considerably more specific and less likely to have adverse effects, and, when utilized as a method for carbohydrate detection, this method is also highly specific and sensitive.
authors.affiliation: Bio-techne, R&D Systems, Inc. 614 McKinley Place N.E. Minneapolis, MN, 55413, USA. Electronic address: Leon.wu@bio-techne.com.
authors.forename: Zhengliang L
authors.initials: ZL
authors.lastname: Wu
authors.affiliation: Bio-techne, R&D Systems, Inc. 614 McKinley Place N.E. Minneapolis, MN, 55413, USA.
authors.forename: Xinyi
authors.initials: X
authors.lastname: Huang
authors.affiliation: Bio-techne, Tocris Bioscience, Tocris House, IO Centre, Moorend Farm Avenue, Bristol, BS11 0QL, UK.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Burton
authors.affiliation: Bio-techne, Tocris Bioscience, Tocris House, IO Centre, Moorend Farm Avenue, Bristol, BS11 0QL, UK.
authors.forename: Karl A D
authors.initials: KA
authors.lastname: Swift
mesh_terms: D000818:Animals; D050260:Carbohydrate Metabolism; D002417:Cattle; D057930:Click Chemistry; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D016695:Glycosyltransferases / Q000378:metabolism; D006801:Humans; D051379:Mice; D019158:N-Acetylneuraminic Acid / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry
chemical_list: D006023:Glycoproteins; D011134:Polysaccharides; D016695:Glycosyltransferases; D019158:N-Acetylneuraminic Acid
keywords: Click chemistry; Glycan detection; Glycosylation; Glycosyltransferase; Protein labeling; Sialyltransferase