title: Effects of Aloe-emodin and Emodin on Proliferation of the MKN45 Human Gastric Cancer Cell Line.
abstract: Aloe-emodin (1, 8-dihydroxy-3-hydroxyl-methylanthraquinone; AE) and emodin (1,3,8-trihydroxy-6- methylanthraquinone; EM) are anthraquinone derivatives that have been detected in some medical plants and share similar anthraquinone structures. AE and EM have been shown to exhibit anticancer activities in various cancer cell lines; however, the inhibitory effects of these derivatives on the growth of cancer cells were previously reported to be different. Gastric cancer is the second most common cause of cancer cell death worldwide. In the present study, we examined the inhibitory effects of 0.05 mM AE and 0.05 mM EM on the proliferation of the MKN45 human gastric cancer cell line. The proliferation of MKN45 cells was significantly inhibited in AE- and EM-treated groups 24 h and 48 h after treatment. Furthermore, the inhibitory effects of EM were stronger than those of AE. The cell cycle of MKN45 cells were arrested in G0/G1 phase or G0/G1 and G2/M phases by AE and EM, respectively. However, an analysis of intracellular polyamine levels and DNA fragmentation revealed that the mechanisms underlying cell death following cell arrest induced by AE and EM differed.
authors.affiliation: Division of Biochemistry, Fujita Memorial Nanakuri Institute, Fujita Health University; 423, Oodori-cho, Tsu, Mie, Japan E-mail : tchihara@fujita-hu.ac.jp.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Chihara
authors.forename: Kan
authors.initials: K
authors.lastname: Shimpo
authors.forename: Hidehiko
authors.initials: H
authors.lastname: Beppu
authors.forename: Naoki
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Takaaki
authors.initials: T
authors.lastname: Kaneko
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Wakamatsu
authors.forename: Shigeru
authors.initials: S
authors.lastname: Sonoda
mesh_terms: D000880:Anthraquinones / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002851:Chromatography, High Pressure Liquid; D053938:DNA Fragmentation / Q000187:drug effects*; D004642:Emodin / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D011073:Polyamines / Q000378:metabolism; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D000880:Anthraquinones; D011073:Polyamines; C518327:aloe emodin; D004642:Emodin