title: Functional analysis of microRNA activity in Brugia malayi.
abstract: The complement of the Brugia malayi microRNA-71 was inserted into the 3' untranslated region of a reporter plasmid, resulting in a decrease in reporter activity. Mutation of the seed sequence restored activity. Insertion of the 3' untranslated regions from two algorithm-predicted putative target genes into the reporter resulted in a similar decrease in activity; mutation of the predicted target sequences restored activity. These experiments demonstrate that B. malayi microRNA targets may be predicted using current algorithms and describe a functional assay to confirm predicted targets.
authors.affiliation: Global Health Infectious Disease Research Program, Department of Global Health, College of Public Health, University of South Florida, 3720 Spectrum Blvd, Suite 304, Tampa, FL 33612, USA.
authors.forename: Canhui
authors.initials: C
authors.lastname: Liu
authors.affiliation: New York Blood Center, Lindsley F. Kimball Research Institute, 310 East 67th Street, New York, NY 10065, USA.
authors.forename: Denis
authors.initials: D
authors.lastname: Voronin
authors.affiliation: New England Biolabs, 240 County Road, Ipswich, MA 01938-2723, USA.
authors.forename: Catherine B
authors.initials: CB
authors.lastname: Poole
authors.affiliation: New York Blood Center, Lindsley F. Kimball Research Institute, 310 East 67th Street, New York, NY 10065, USA.
authors.forename: Saheed
authors.initials: S
authors.lastname: Bachu
authors.affiliation: Department of Surgery, University of Pittsburgh, Children's Hospital, Pittsburgh, USA.
authors.forename: Matthew B
authors.initials: MB
authors.lastname: Rogers
authors.affiliation: New England Biolabs, 240 County Road, Ipswich, MA 01938-2723, USA.
authors.forename: Jingmin
authors.initials: J
authors.lastname: Jin
authors.affiliation: Department of Biology, Center for Genomics & Systems Biology, Global Institute of Public Health, New York University, New York, NY 10003, USA.
authors.forename: Elodie
authors.initials: E
authors.lastname: Ghedin
authors.affiliation: New York Blood Center, Lindsley F. Kimball Research Institute, 310 East 67th Street, New York, NY 10065, USA.
authors.forename: Sara
authors.initials: S
authors.lastname: Lustigman
authors.affiliation: New England Biolabs, 240 County Road, Ipswich, MA 01938-2723, USA.
authors.forename: Larry A
authors.initials: LA
authors.lastname: McReynolds
authors.affiliation: Global Health Infectious Disease Research Program, Department of Global Health, College of Public Health, University of South Florida, 3720 Spectrum Blvd, Suite 304, Tampa, FL 33612, USA. Electronic address: tunnasch@health.usf.edu.
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Unnasch
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D017178:Brugia malayi / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D005819:Genetic Markers; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*
chemical_list: D005819:Genetic Markers; D018840:HSP70 Heat-Shock Proteins; D035683:MicroRNAs; D008156:Luciferases
keywords: Filariasis; Genetic regulation; Transfection; miRNA