title: Spectroscopic analyses on interaction of melamine, cyanuric acid and uric acid with DNA.
abstract: In this work, the interaction of DNA with melamine (MEL), cyanuric acid (CYA) and uric acid (UA) were studied, respectively, by means of UV-vis, fluorescence, circular dichroism (CD) spectroscopy, viscosity and gel electrophoresis methods. The fluorescence quenching was used to study the interaction models of MEL, CYA and UA with DNA, respectively, and the bimolecular quenching constant (Kq), apparent quenching constant (Ksv), effective binding constant (KA) and corresponding dissociation constant (KD) and binding site number (n) were calculated by adopting Stern-Volmer, Lineweaver-Burk and Double logarithm equations. The results show that MEL, CYA and UA are all able to markedly bind to DNA, and the binding strength order is DNA-UA>DNA-CYA>DNA-MEL. It is wished that these researches would facilitate the understanding of the formation of kidney stones and gout in the body after ingesting excess MEL.
authors.affiliation: College of Chemistry, Liaoning University, Shenyang 110036, PR China.
authors.forename: Jinhui
authors.initials: J
authors.lastname: Xie
authors.affiliation: College of Chemistry, Liaoning University, Shenyang 110036, PR China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Chen
authors.affiliation: College of Chemistry, Liaoning University, Shenyang 110036, PR China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
authors.affiliation: College of Chemistry, Liaoning University, Shenyang 110036, PR China. Electronic address: wangjun888tg@126.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Environment, Liaoning University, Shenyang 110036, PR China.
authors.forename: Heng
authors.initials: H
authors.lastname: Qiao
mesh_terms: D001599:Berberine / Q000737:chemistry; D001667:Binding, Competitive; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D007700:Kinetics; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014227:Triazines / Q000737:chemistry / Q000378:metabolism*; D014527:Uric Acid / Q000737:chemistry / Q000378:metabolism*; D014783:Viscosity
chemical_list: D012996:Solutions; D014227:Triazines; D001599:Berberine; D014527:Uric Acid; D004247:DNA; C004632:cyanuric acid; C011907:melamine
keywords: Desoxyribonucleic acid (DNA); Interaction; Melamine; Spectroscopy; Viscosity method