title: Confirmation of the absence of tetrodotoxin and its analogues in the juveniles of the Japanese fire-bellied newt, Cynops pyrrhogaster, captive-reared from eggs in the laboratory using HILIC-LC-MS.
abstract: The tetrodotoxin (TTX) contents of the Japanese fire-bellied newt, Cynops pyrrhogaster, captive-reared from eggs to metamorphosed juveniles with a non-toxic diet for 70 weeks, as well as wild-caught juvenile newts, were investigated using a high-resolution hydrophilic interaction chromatography-LC-MS. TTX was detected in 0- to 22-week-old captive-reared juvenile newts but was not detected (<15 ng/g) in the 36- to 70-week-old newts, while significant levels of TTX (1.3-14 μg/g) were detected in the wild-caught juveniles.
authors.affiliation: Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai, Miyagi, 981-8555, Japan.
authors.forename: Yuta
authors.initials: Y
authors.lastname: Kudo
authors.affiliation: Faculty of Life and Environmental Sciences, University of Tsukuba, Tennoudai 1-1-1, Tsukuba, Ibaraki, 305-8572, Japan.
authors.forename: Chikafumi
authors.initials: C
authors.lastname: Chiba
authors.affiliation: Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai, Miyagi, 981-8555, Japan.
authors.forename: Keiichi
authors.initials: K
authors.lastname: Konoki
authors.affiliation: Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai, Miyagi, 981-8555, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Cho
authors.affiliation: Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai, Miyagi, 981-8555, Japan. Electronic address: myama@biochem.tohoku.ac.jp.
authors.forename: Mari
authors.initials: M
authors.lastname: Yotsu-Yamashita
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D005260:Female; D013058:Mass Spectrometry / Q000379:methods*; D008675:Metamorphosis, Biological / Q000187:drug effects; D010063:Ovum / Q000187:drug effects*; D012455:Salamandridae / Q000378:metabolism*; D013779:Tetrodotoxin / Q000032:analysis*
chemical_list: D013779:Tetrodotoxin
keywords: Cynops pyrrhogaster; LC-MS; Newt; Tetrodotoxin; Toxicity analysis