title: Modifying glycoalkaloid content in transgenic potato--Metabolome impacts.
abstract: Metabolite profiling has been used to assess the potential for unintended composition changes in potato (Solanum tuberosum L. cv. Desirée) tubers, which have been genetically modified (GM) to reduce glycoalkaloid content, via the independent down-regulation of three genes SGT1, SGT2 and SGT3 known to be involved in glycoalkaloid biosynthesis. Differences between the three groups of antisense lines and control lines were assessed using liquid chromatography-mass spectrometry (LC-MS) and gas chromatography (GC)-MS, and data analysed using principal component analysis and analysis of variance. Compared with the wild-type (WT) control, LC-MS revealed not only the expected changes in specific glycoalkaloid levels in the GM lines, but also significant changes in several other metabolites, some of which were explicable in terms of known pathways. Analysis of polar and non-polar metabolites by GC-MS revealed other significant (unintended) differences between SGT lines and the WT, but also between the WT control and other control lines used.
authors.affiliation: Environmental and Biochemical Sciences Group, The James Hutton Institute, Invergowrie, Dundee DD2 5DA, Scotland, United Kingdom. Electronic address: louise.shepherd@hutton.ac.uk.
authors.forename: Louise Vida Traill
authors.initials: LV
authors.lastname: Shepherd
authors.affiliation: Biomathematics and Statistics Scotland, Invergowrie, Dundee DD2 5DA, Scotland, United Kingdom.
authors.forename: Christine Anne
authors.initials: CA
authors.lastname: Hackett
authors.affiliation: Biomathematics and Statistics Scotland, Invergowrie, Dundee DD2 5DA, Scotland, United Kingdom.
authors.forename: Colin James
authors.initials: CJ
authors.lastname: Alexander
authors.affiliation: Biomathematics and Statistics Scotland, Invergowrie, Dundee DD2 5DA, Scotland, United Kingdom.
authors.forename: James William
authors.initials: JW
authors.lastname: McNicol
authors.affiliation: Environmental and Biochemical Sciences Group, The James Hutton Institute, Invergowrie, Dundee DD2 5DA, Scotland, United Kingdom.
authors.forename: Julia Anne
authors.initials: JA
authors.lastname: Sungurtas
authors.affiliation: Environmental and Biochemical Sciences Group, The James Hutton Institute, Invergowrie, Dundee DD2 5DA, Scotland, United Kingdom; School of Life Sciences, Heriot-Watt University, Edinburgh EH14 4AS, Scotland, United Kingdom.
authors.forename: Derek
authors.initials: D
authors.lastname: Stewart
authors.affiliation: USDA, Agricultural Research Service, Crop Improvement and Genetics, Western Regional Research Centre, 800 Buchanan St, Albany, CA 94710-1105, USA.
authors.forename: Kent Frank
authors.initials: KF
authors.lastname: McCue
authors.affiliation: USDA, Agricultural Research Service, Crop Improvement and Genetics, Western Regional Research Centre, 800 Buchanan St, Albany, CA 94710-1105, USA.
authors.forename: William Richardson
authors.initials: WR
authors.lastname: Belknap
authors.affiliation: Environmental and Biochemical Sciences Group, The James Hutton Institute, Invergowrie, Dundee DD2 5DA, Scotland, United Kingdom.
authors.forename: Howard Vivian
authors.initials: HV
authors.lastname: Davies
mesh_terms: D002849:Chromatography, Gas; D015536:Down-Regulation; D005838:Genotype; D016695:Glycosyltransferases / Q000235:genetics*; D013058:Mass Spectrometry; D055442:Metabolome / Q000235:genetics; D035281:Plant Tubers / Q000737:chemistry / Q000201:enzymology / Q000235:genetics; D030821:Plants, Genetically Modified / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism*; D012991:Solanaceous Alkaloids / Q000032:analysis* / Q000096:biosynthesis; D011198:Solanum tuberosum / Q000737:chemistry / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*
chemical_list: D012991:Solanaceous Alkaloids; D016695:Glycosyltransferases
keywords: Genetic modification; Glycoalkaloids; Metabolomics; Potato (Solanum tuberosum L.); SGT; Transgenic