title: Genome-wide analysis of positively selected genes in seasonal and non-seasonal breeding species.
abstract: Some mammals breed throughout the year, while others breed only at certain times of year. These differences in reproductive behavior can be explained by evolution. We identified positively-selected genes in two sets of species with different degrees of relatedness including seasonal and non-seasonal breeding species, using branch-site models. After stringent filtering by sum of pairs scoring, we revealed that more genes underwent positive selection in seasonal compared with non-seasonal breeding species. Positively-selected genes were verified by cDNA mapping of the positive sites with the corresponding cDNA sequences. The design of the evolutionary analysis can effectively lower the false-positive rate and thus identify valid positive genes. Validated, positively-selected genes, including CGA, DNAH1, INVS, and CD151, were related to reproductive behaviors such as spermatogenesis and cell proliferation in non-seasonal breeding species. Genes in seasonal breeding species, including THRAP3, TH1L, and CMTM6, may be related to the evolution of sperm and the circadian rhythm system. Identification of these positively-selected genes might help to identify the molecular mechanisms underlying seasonal and non-seasonal reproductive behaviors.
authors.affiliation: School of Bioscience and Bioengineering, Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, South China University of Technology, Guangzhou, China.
authors.forename: Yuhuan
authors.initials: Y
authors.lastname: Meng
authors.affiliation: School of Bioscience and Bioengineering, Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, South China University of Technology, Guangzhou, China.
authors.forename: Wenlu
authors.initials: W
authors.lastname: Zhang
authors.affiliation: School of Bioscience and Bioengineering, Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, South China University of Technology, Guangzhou, China.
authors.forename: Jinghui
authors.initials: J
authors.lastname: Zhou
authors.affiliation: School of Bioscience and Bioengineering, Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, South China University of Technology, Guangzhou, China.
authors.forename: Mingyu
authors.initials: M
authors.lastname: Liu
authors.affiliation: School of Bioscience and Bioengineering, Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, South China University of Technology, Guangzhou, China.
authors.forename: Junhui
authors.initials: J
authors.lastname: Chen
authors.affiliation: School of Bioscience and Bioengineering, Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, South China University of Technology, Guangzhou, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Tian
authors.affiliation: School of Bioscience and Bioengineering, Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, South China University of Technology, Guangzhou, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhuo
authors.affiliation: Guangdong Key Laboratory of Laboratory Animals/Guangdong laboratory animals monitoring institution, Guangzhou, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Life Sciences, Fudan University, Shanghai, China; Institute of Biodiversity Science, Tibet University, Lhasa, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhong
authors.affiliation: School of Bioscience and Bioengineering, Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, South China University of Technology, Guangzhou, China.
authors.forename: Hongli
authors.initials: H
authors.lastname: Du
authors.affiliation: School of Bioscience and Bioengineering, Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, South China University of Technology, Guangzhou, China; Chinese PLA General Hospital, Beijing, China.
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D002940:Circadian Rhythm / Q000235:genetics; D019143:Evolution, Molecular; D005260:Female; D005786:Gene Expression Regulation; D016678:Genome; D000882:Haplorhini; D006801:Humans; D008297:Male; D051379:Mice; D051381:Rats; D012098:Reproduction / Q000235:genetics*; D012621:Seasons; D012641:Selection, Genetic; D013091:Spermatogenesis / Q000235:genetics