title: Extracellular matrix protein reelin regulate dendritic spine density through CaMKIIβ.
abstract: Reelin, an extracellular matrix protein, plays an important role in brain development as well as synaptic plasticity. Interestingly, several recent studies have found that Reelin is important for dendritic spine formation in vitro and in vivo. However, the molecular mechanism by which Reelin regulates the dendritic spine density has not been studied well yet. In this study, we found that exogenous Reelin treatment was significantly increased the dendritic spine density in the primary hippocampal neurons. In addition, Reelin was increased the puncta numbers of synaptophysin and PSD-95. Moreover, we found that Reelin modulated the levels of CaMKIIβ, and CaMKIIβ siRNA prevented Reelin's effect on the dendritic spine density. Overall, our results are the first to demonstrate that CaMKIIβ might be required to enable Reelin to alter the dendritic spine density.
authors.affiliation: Department of Physical Therapy, Inje University, Gimhae, 621-749, Republic of Korea.
authors.forename: Mihyun
authors.initials: M
authors.lastname: Kim
authors.affiliation: Department of Physical Therapy, Inje University, Gimhae, 621-749, Republic of Korea; Research Institute of Biomedical Engineering and Department of Medicine, Catholic University of Daegu School of Medicine, Daegu 705-718, Republic of Korea.
authors.forename: Yun
authors.initials: Y
authors.lastname: Jeong
authors.affiliation: Research Institute of Biomedical Engineering and Department of Medicine, Catholic University of Daegu School of Medicine, Daegu 705-718, Republic of Korea. Electronic address: ycchang@cu.ac.kr.
authors.forename: Young-Chae
authors.initials: YC
authors.lastname: Chang
mesh_terms: D000818:Animals; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000378:metabolism*; D015816:Cell Adhesion Molecules, Neuronal / Q000378:metabolism* / Q000494:pharmacology; D002478:Cells, Cultured; D049229:Dendritic Spines / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D016326:Extracellular Matrix Proteins / Q000378:metabolism* / Q000494:pharmacology; D006624:Hippocampus / Q000166:cytology; D006801:Humans; D009419:Nerve Tissue Proteins / Q000378:metabolism* / Q000494:pharmacology; D017207:Rats, Sprague-Dawley; D000091042:Reelin Protein; D012697:Serine Endopeptidases / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D016326:Extracellular Matrix Proteins; D009419:Nerve Tissue Proteins; D000091042:Reelin Protein; C000717129:Reln protein, rat; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; C000717127:RELN protein, human; D012697:Serine Endopeptidases
keywords: CaMKIIβ; Dendritic Spine; Hippocampus; Reelin