title: Expression of Peroxiredoxin 1 After Traumatic Spinal Cord Injury in Rats.
abstract: Reactive astrogliosis and microgliosis after spinal cord injury (SCI) contribute to glial scar formation that impedes axonal regeneration. The mechanisms underlying reactive astrocyte and microglia proliferation upon injury remain partially understood. Peroxiredoxin 1 (PRDX1) is an antioxidant participating in cell proliferation, differentiation, and apoptosis. However, PRDX1 functions in SCI-induced astrocyte and microglia proliferation are unknown. In this study, we established an acute spinal cord contusion injury model in adult rats to investigate the potential role of PRDX1 during the pathological process of SCI. We found the palpable expression increase of PRDX1 after SCI by western blot and immunohistochemistry staining. Double immunofluorescence staining showed that PRDX1 expression mainly increased in astrocytes and microglia. In addition, PRDX1/proliferating cell nuclear antigen (PCNA) colocalized in astrocytes and microglia. Furthermore, PCNA expression also elevated after SCI, as well as was positively correlated with PRDX1 expression. In vitro, PRDX1 expression in primary rat spinal cord astrocytes and microglia changed in a concentration- and time-dependent manner according to LPS treatment. In addition, PRDX1 knockdown in astrocytes and microglia resulted in the decrease of PCNA expression after LPS stimulation, showing that PRDX1 promoted astrocyte and microglia proliferation after inflammation. Our results suggested that PRDX1 might play a crucial role in astrocyte and microglia proliferation after SCI.
authors.affiliation: Department of Spine Surgery, The Second Affiliated Hospital, Nantong University, Nantong, 226001, People's Republic of China.|Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.
authors.forename: Shen
authors.initials: S
authors.lastname: Huang
authors.affiliation: Department of Pathogen Biology, Medical College, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.|Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Spine Surgery, The Second Affiliated Hospital, Nantong University, Nantong, 226001, People's Republic of China.|Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.
authors.forename: Jinlong
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Spine Surgery, The Second Affiliated Hospital, Nantong University, Nantong, 226001, People's Republic of China.
authors.forename: Guofeng
authors.initials: G
authors.lastname: Bao
authors.affiliation: Department of Spine Surgery, The Second Affiliated Hospital, Nantong University, Nantong, 226001, People's Republic of China.
authors.forename: Guanhua
authors.initials: G
authors.lastname: Xu
authors.affiliation: Department of Spine Surgery, The Second Affiliated Hospital, Nantong University, Nantong, 226001, People's Republic of China.
authors.forename: Yuyu
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Stomatology, Affiliated Hospital of Nantong University, Nantong, 226001, People's Republic of China.|Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.
authors.forename: Qijie
authors.initials: Q
authors.lastname: Shen
authors.affiliation: Department of Stomatology, Affiliated Hospital of Nantong University, Nantong, 226001, People's Republic of China.|Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Lian
authors.affiliation: Institute of Navigation Medicine, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.|Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.
authors.forename: Yuwei
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Spine Surgery, The Second Affiliated Hospital, Nantong University, Nantong, 226001, People's Republic of China. cuizhiming2012@hotmail.com.|Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China. cuizhiming2012@hotmail.com.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Cui
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism / Q000473:pathology; D049109:Cell Proliferation / Q000502:physiology; D005786:Gene Expression Regulation; D008297:Male; D017628:Microglia / Q000378:metabolism / Q000473:pathology; D054464:Peroxiredoxins / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000378:metabolism* / Q000473:pathology
chemical_list: C489898:Prdx1 protein, rat; D054464:Peroxiredoxins
keywords: Glial cell proliferation; Peroxiredoxin 1 (PRDX1); Rats; Spinal cord injury (SCI)