title: Application of dual tree complex wavelet transform in tandem mass spectrometry.
abstract: Mass Spectrometry (MS) is a widely used technique in molecular biology for high throughput identification and sequencing of peptides (and proteins). Tandem mass spectrometry (MS/MS) is a specialised mass spectrometry technique whereby the sequence of peptides can be determined. Preprocessing of the MS/MS data is indispensable before performing any statistical analysis on the data. In this work, preprocessing of MS/MS data is proposed based on the Dual Tree Complex Wavelet Transform (DTCWT) using almost symmetric Hilbert pair of wavelets. After the preprocessing step, the identification of peptides is done using the database search approach. The performance of the proposed preprocessing technique is evaluated by comparing its performance against Discrete Wavelet Transform (DWT) and Stationary Wavelet Transform (SWT). The preprocessing performed using DTCWT identified more peptides compared to DWT and SWT.
authors.affiliation: Department of Electronic Engineering, La Trobe University, Bundoora, Victoria 3086, Australia. Electronic address: s.murugesan@latrobe.edu.au.
authors.forename: Selvaraaju
authors.initials: S
authors.lastname: Murugesan
authors.affiliation: Department of Electronic Engineering, La Trobe University, Bundoora, Victoria 3086, Australia. Electronic address: d.tay@latrobe.edu.au.
authors.forename: David B H
authors.initials: DB
authors.lastname: Tay
authors.affiliation: Department of Biochemistry, La Trobe University, Bundoora, Victoria 3086, Australia. Electronic address: i.cooke@latrobe.edu.au.
authors.forename: Ira
authors.initials: I
authors.lastname: Cooke
authors.affiliation: Department of Biochemistry, La Trobe University, Bundoora, Victoria 3086, Australia. Electronic address: p.faou@latrobe.edu.au.
authors.forename: Pierre
authors.initials: P
authors.lastname: Faou
mesh_terms: D030562:Databases, Protein; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008956:Models, Chemical; D009363:Neoplasm Proteins / Q000032:analysis / Q000378:metabolism; D009369:Neoplasms / Q000737:chemistry / Q000378:metabolism; D010455:Peptides / Q000032:analysis / Q000378:metabolism
chemical_list: D009363:Neoplasm Proteins; D010455:Peptides
keywords: Dual tree complex wavelet transform; Peptides detection; Proteomic data processing; Signal denoising; Tandem mass spectrometry