title: Effects of selenium on endothelial dysfunction and metabolic profile in low dose streptozotocin induced diabetic rats fed a high fat diet.
abstract: Endothelial dysfunction develops as a result of oxidative stress and is responsible for diabetic vascular complications. We investigated the effects of selenium on endothelial dysfunction and oxidative stress in type 2 diabetic rats. Male Wistar rats were divided into five groups: controls, untreated diabetics, and diabetics treated with 180, 300, 500 mcg/kg selenium each day. Diabetes was induced by a single intraperitoneal injection of low dose streptozotocin to rats fed a high fat diet. Endothelium-dependent and -independent relaxations were measured in the thoracic aorta. Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase and endothelial nitric oxide synthase (eNOS) mRNA expressions were analyzed using real-time polymerase chain reaction (RT-PCR). Fasting blood glucose, lipid profile, lipid oxidation, insulin and nitric oxide were measured in blood samples. Malondialdehyde, superoxide dismutase, catalase and glutathione peroxidase levels were measured in liver samples. RT-PCR showed that selenium reversed increased NADPH oxidase expression and decreased eNOS expression to control levels. Selenium also improved the impairment of endothelium-dependent vasorelaxation in the diabetic aorta. Selenium treatment significantly decreased blood glucose, cholesterol and triglyceride levels, and enhanced the antioxidant status in diabetic rats. Our findings suggest that selenium restores a normal metabolic profile and ameliorates vascular responses and endothelial dysfunction in diabetes by regulating antioxidant enzyme and nitric oxide release.
authors.affiliation: a Department of Clinical Pharmacology and Toxicology , Atatürk Research Hospital , Izmir , Turkey.
authors.forename: Z
authors.initials: Z
authors.lastname: Oztürk
authors.affiliation: b Department of Medical Pharmacology , Faculty of Medicine, Celal Bayar University , Manisa , Turkey.
authors.forename: T
authors.initials: T
authors.lastname: Gurpinar
authors.affiliation: b Department of Medical Pharmacology , Faculty of Medicine, Celal Bayar University , Manisa , Turkey.
authors.forename: K
authors.initials: K
authors.lastname: Vural
authors.affiliation: c Department of Medical Genetics , Faculty of Medicine, Celal Bayar University , Manisa , Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Boyacıoglu
authors.affiliation: d Department of Medical Biology , Faculty of Medicine, Celal Bayar University , Manisa , Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Korkmaz
authors.affiliation: e Department of Biochemistery , Faculty of Medicine, Celal Bayar University , Manisa , Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Var
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000378:metabolism; D059305:Diet, High-Fat; D004727:Endothelium / Q000187:drug effects*; D008297:Male; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism*; D017208:Rats, Wistar; D012643:Selenium / Q000494:pharmacology*; D013311:Streptozocin
chemical_list: D000975:Antioxidants; D013311:Streptozocin; D052250:Nitric Oxide Synthase Type III; D012643:Selenium
keywords: antioxidant; diabetes; endothelial dysfunction; high fat diet; nitric oxide; rat