title: Rapid and efficient differentiation of Yersinia species using high-resolution melting analysis.
abstract: The primary goal of clinical microbiology is the accurate identification of the causative agent of the disease. Here, we describe a method for differentiation between Yersinia species using PCR-HRMA. The results revealed species-specific melting profiles. The herein developed assay can be used as an effective method to differentiate Yersinia species.
authors.affiliation: Brazilian Reference Center on Yersinia spp. other than Y. pestis, Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto-USP, Ribeirão Preto, SP, Brazil.
authors.forename: Roberto A
authors.initials: RA
authors.lastname: Souza
authors.affiliation: Brazilian Reference Center on Yersinia spp. other than Y. pestis, Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto-USP, Ribeirão Preto, SP, Brazil.
authors.forename: Miliane R
authors.initials: MR
authors.lastname: Frazão
authors.affiliation: Brazilian Reference Service on Plague, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz (CPqAM/FIOCRUZ), Recife, PE, Brazil.
authors.forename: Alzira M P
authors.initials: AM
authors.lastname: Almeida
authors.affiliation: Brazilian Reference Center on Yersinia spp. other than Y. pestis, Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto-USP, Ribeirão Preto, SP, Brazil. Electronic address: jufalcao@fcfrp.usp.br.
authors.forename: Juliana P
authors.initials: JP
authors.lastname: Falcão
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000737:chemistry* / Q000235:genetics; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D044366:Transition Temperature; D015007:Yersinia / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D015009:Yersinia Infections / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial
keywords: 16S rRNA gene; Molecular diagnosis; PCR-HRMA; Yersinia species differentiation