title: Isolation and expression of HMG-CoA synthase and HMG-CoA reductase genes in different development stages, tissues and treatments of the Chinese white pine beetle, Dendroctonus armandi (Curculionidae: Scolytinae).
abstract: We isolated two full-length cDNAs encoding 3-hydroxy-3-methyl-glutaryl coenzyme A synthase (HMG-S) and 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-R) from the Chinese white pine beetle (Dendroctonus armandi), and carried out some bioinformatic analysis on the full-length nucleic acid sequences and deduced amino acid sequences. Differential expression of the DaHMG-S and DaHMG-R genes was observed between sexes (emerged adults), and within these significant differences among development stage, tissue distribution, fed on phloem of Pinus armandi and topically applied juvenile hormone (JH) III. Increase of DaHMG-S and DaHMG-R mRNA levels in males suggested that they may play a role in mevalonate pathway. Information from the present study might contribute to understanding the relationship between D. armandi and its semiochemical production.
authors.affiliation: College of Forestry, Northwest A and F University, Yangling, 712100 Shaanxi, China.
authors.forename: Jiamin
authors.initials: J
authors.lastname: Yu
authors.affiliation: College of Forestry, Northwest A and F University, Yangling, 712100 Shaanxi, China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Dai
authors.affiliation: College of Forestry, Northwest A and F University, Yangling, 712100 Shaanxi, China.
authors.forename: Ranran
authors.initials: R
authors.lastname: Zhang
authors.affiliation: College of Forestry, Northwest A and F University, Yangling, 712100 Shaanxi, China.
authors.forename: Zhumei
authors.initials: Z
authors.lastname: Li
authors.affiliation: College of Forestry, Northwest A and F University, Yangling, 712100 Shaanxi, China.
authors.forename: Thanh
authors.initials: T
authors.lastname: Pham
authors.affiliation: College of Forestry, Northwest A and F University, Yangling, 712100 Shaanxi, China. Electronic address: chenhui@nwsuaf.edu.cn.
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019295:Computational Biology; D004435:Eating; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D006903:Hydroxymethylglutaryl CoA Reductases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D006904:Hydroxymethylglutaryl-CoA Synthase / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D008297:Male; D008798:Mevalonic Acid / Q000378:metabolism; D008969:Molecular Sequence Data; D021381:Protein Transport / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012717:Sesquiterpenes / Q000494:pharmacology; D048889:Weevils / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development* / Q000502:physiology
chemical_list: D012333:RNA, Messenger; D012717:Sesquiterpenes; C036585:juvenile hormone III; D006903:Hydroxymethylglutaryl CoA Reductases; D006904:Hydroxymethylglutaryl-CoA Synthase; D008798:Mevalonic Acid
keywords: DaHMG-R; DaHMG-S; Feeding; Juvenile hormone (JH) III; Transcript levels