title: A terpenoid phytoalexin plays a role in basal defense of Nicotiana benthamiana against Potato virus X.
abstract: Terpenoid phytoalexins function as defense compound against a broad spectrum of pathogens and pests in the plant kingdom. However, the role of phytoalexin in antiviral defense is still elusive. In this study, we identified the biosynthesis pathway of a sesquiterpenoid phytoalexin, capsidiol 3-acetate as an antiviral response against RNA virus Potato Virus X (PVX) in Nicotiana benthamiana. NbTPS1 and NbEAH genes were found strongly induced by PVX-infection. Enzymatic activity and genetic evidence indicated that both genes were involved in the PVX-induced biosynthesis of capsidiol 3-acetate. NbTPS1- or NbEAH-silenced plant was more susceptible to PVX. The accumulation of capsidiol 3-acetate in PVX-infected plant was partially regulated by jasmonic acid signaling receptor COI1. These findings provide an insight into a novel mechanism of how plant uses the basal arsenal machinery to mount a fight against virus attack even in susceptible species.
authors.affiliation: Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117604, Singapore.
authors.forename: Ran
authors.initials: R
authors.lastname: Li
authors.affiliation: Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117604, Singapore.
authors.forename: Chuan-Sia
authors.initials: CS
authors.lastname: Tee
authors.affiliation: Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117604, Singapore.
authors.forename: Yu-Lin
authors.initials: YL
authors.lastname: Jiang
authors.affiliation: Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117604, Singapore.
authors.forename: Xi-Yuan
authors.initials: XY
authors.lastname: Jiang
authors.affiliation: Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117604, Singapore.
authors.forename: Prasanna Nori
authors.initials: PN
authors.lastname: Venkatesh
authors.affiliation: Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117604, Singapore.
authors.forename: Rajani
authors.initials: R
authors.lastname: Sarojam
authors.affiliation: 1] Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117604, Singapore [2] State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Ye
mesh_terms: D053898:Biosynthetic Pathways / Q000235:genetics; D020868:Gene Silencing; D010935:Plant Diseases / Q000821:virology; D010940:Plant Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D017863:Potexvirus / Q000187:drug effects* / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D012717:Sesquiterpenes / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D013729:Terpenes / Q000737:chemistry*; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism / Q000821:virology*; D000094162:Phytoalexins
chemical_list: D010940:Plant Proteins; D011994:Recombinant Proteins; D012717:Sesquiterpenes; D013729:Terpenes; C081843:capsidiol; C054277:aristolochene; D000094162:Phytoalexins