title: Amniotic membrane is a potential regenerative option for chronic non-healing wounds: a report of five cases receiving dehydrated human amnion/chorion membrane allograft.
abstract: A case series of five patients with a total of six chronic non-healing wounds (>30 day duration) were non-randomly selected to evaluate the performance, safety and handling properties of dehydrated human amnion/chorion membrane allograft, an amniotic membrane scaffolding product. The patients had lower extremity wounds that had previously failed standard of care within a university outpatient/inpatient wound healing programme. Five wounds treated with dehydrated amnion/chorion membrane allograft showed a mean 43% area reduction from baseline (51% median) at 3 weeks into treatment and completely healed with a 64-day median time to closure (SD ±27·6 days). One wound worsened at 3 weeks and was found to have a complete central vein obstruction that was treated with long-term mild compression but still eventually healed at 6 months. Removing this outlier, the four responding wounds had a 72% mean and 69% median change in area from baseline, at the 3 week point. All five patients received only one application of dehydrated human amnion/chorion membrane allograft, and there were no adverse events. The product was easy to use, administer and handle. In summary, dehydrated human amnion/chorion membrane allograft appears to be a safe, effective and easy to use therapy for chronic non-healing wounds. This study describes the details of these clinical cases and provides an overview of the current evidence on the use of amniotic tissue in clinical practice.
authors.affiliation: Saint Louis University School of Medicine, St. Louis, MO, USA.
authors.forename: Andrew
authors.initials: A
authors.lastname: Mrugala
authors.affiliation: Chicago College Osteopathic Medicine, Midwestern University, Downers Grove, IL, USA.|Division of Vascular Surgery, Section of Wound Healing and Tissue Repair, University of Illinois Hospital and Health Sciences System, Chicago, IL, USA.
authors.forename: Audrey
authors.initials: A
authors.lastname: Sui
authors.affiliation: Division of Vascular Surgery, Section of Wound Healing and Tissue Repair, University of Illinois Hospital and Health Sciences System, Chicago, IL, USA.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Plummer
authors.affiliation: Division of Vascular Surgery, Section of Wound Healing and Tissue Repair, University of Illinois Hospital and Health Sciences System, Chicago, IL, USA.
authors.forename: Igor
authors.initials: I
authors.lastname: Altman
authors.affiliation: Division of Vascular Surgery, Section of Wound Healing and Tissue Repair, University of Illinois Hospital and Health Sciences System, Chicago, IL, USA.
authors.forename: Elaine
authors.initials: E
authors.lastname: Papineau
authors.affiliation: Division of Vascular Surgery, Section of Wound Healing and Tissue Repair, University of Illinois Hospital and Health Sciences System, Chicago, IL, USA.|Department of Wound Healing, Grand View Hospital, Sellersville, PA, USA.
authors.forename: Devn
authors.initials: D
authors.lastname: Frandsen
authors.affiliation: Division of Vascular Surgery, Section of Wound Healing and Tissue Repair, University of Illinois Hospital and Health Sciences System, Chicago, IL, USA.
authors.forename: Danielle
authors.initials: D
authors.lastname: Hill
authors.affiliation: Division of Vascular Surgery, Section of Wound Healing and Tissue Repair, University of Illinois Hospital and Health Sciences System, Chicago, IL, USA.
authors.forename: William J
authors.initials: WJ
authors.lastname: Ennis
mesh_terms: D064591:Allografts; D000650:Amnion; D002823:Chorion; D006801:Humans; D035002:Lower Extremity; D014945:Wound Healing
keywords: Amniotic membrane; Chronic wound healing; Dehydrated human amnion/chorion membrane allograft