title: Expansion of NK cells from PBMCs using immobilized 4-1BBL and interleukin-21.
abstract: Adoptive transfer of NK cells has been widely applied clinically for cancer immunotherapy. However, the difficulties to obtain a large number of activated NK cells impede the successful application of such therapy. In the present study, we implemented a novel method involving the use of immobilized human 4-1BBL and interleukin-21 to amplify NK cells from the peripheral blood mononuclear cells (PBMCs) of healthy donors. Following stimulation for 21 days, we achieved considerable expansion of NK cells with high purity and strong cytotoxicity. This is the first time solid phase cytokines were used to augment NK cells, and this method has the advantage of no need to introduce feeder cells, without prior purification of NK cells and it effectively stimulated and expanded NK cells. The strategy of cell proliferation and activation could lead to a safer and more effective application of NK cells clinically.
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology (CASPMI), Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, P.R. China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Li
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology (CASPMI), Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, P.R. China.
authors.forename: Chenhui
authors.initials: C
authors.lastname: He
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology (CASPMI), Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, P.R. China.
authors.forename: Changzhen
authors.initials: C
authors.lastname: Liu
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology (CASPMI), Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, P.R. China.
authors.forename: Juan
authors.initials: J
authors.lastname: Ma
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology (CASPMI), Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, P.R. China.
authors.forename: Pan
authors.initials: P
authors.lastname: Ma
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology (CASPMI), Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, P.R. China.
authors.forename: Honglian
authors.initials: H
authors.lastname: Cui
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology (CASPMI), Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, P.R. China.
authors.forename: Hua
authors.initials: H
authors.lastname: Tao
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology (CASPMI), Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, P.R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Gao
mesh_terms: D053330:4-1BB Ligand / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D019921:Biotinylation; D049109:Cell Proliferation; D006801:Humans; D061925:Immobilized Nucleic Acids; D055767:Immobilized Proteins / Q000096:biosynthesis; D007378:Interleukins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D007694:Killer Cells, Natural / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D008213:Lymphocyte Activation; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D000098546:Interleukin-21
chemical_list: D053330:4-1BB Ligand; D061925:Immobilized Nucleic Acids; D055767:Immobilized Proteins; D007378:Interleukins; D011994:Recombinant Proteins; D000098546:Interleukin-21