title: Identification and quantification of acidosis inducing metabolites in cases of alcohols intoxication by GC-MS for emergency toxicology.
abstract: A simple, cost effective, and fast gas chromatography method with mass spectrometry detection (GC-MS) for simultaneous measurement of formic acid, glycolic acid, methoxyacetic acid, ethoxyacetic acid and 2-hydroxyethoxyacetic acid in serum and urine was developed and validated. This multi-analyte method is highly suitable for clinical and emergency toxicology laboratory diagnostic, allowing identification and quantification of five most common acidosis inducing organic acids present in cases of alcohol intoxication. Furthermore, when patients are admitted to emergency unit at late stage of toxic alcohol intoxication, the concentration of parent compound may be already low or not detectable. This new method employs a relatively less used class of derivatization agents - alkyl chloroformates, allowing the efficient and rapid derivatization of carboxylic acids within seconds. The entire sample preparation procedure is completed within 5 min. The optimal conditions of derivatization procedure have been found using chemometric approach (design of experiment). The calibration dependence of the method was proved to be quadratic in the range of 25-3000 mg L(-1), with adequate accuracy (97.3-108.0%) and precision (<12.8%). The method was successfully applied for identification and quantification of the selected compounds in serum of patients from emergency units.
authors.affiliation: Institute of Forensic Medicine and Toxicology, First Faculty of Medicine, Charles University in Prague and General University Hospital, 121 08 Prague 2, Czech Republic. Electronic address: tomas.hlozek@vfn.cz.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Hložek
authors.affiliation: Institute of Forensic Medicine and Toxicology, First Faculty of Medicine, Charles University in Prague and General University Hospital, 121 08 Prague 2, Czech Republic; Department of Analytical Chemistry, Faculty of Science, Charles University in Prague, Albertov 6, 128 43 Prague 2, Czech Republic.
authors.forename: Miroslava
authors.initials: M
authors.lastname: Bursová
authors.affiliation: Department of Analytical Chemistry, Faculty of Science, Charles University in Prague, Albertov 6, 128 43 Prague 2, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Coufal
authors.affiliation: Institute of Forensic Medicine and Toxicology, First Faculty of Medicine, Charles University in Prague and General University Hospital, 121 08 Prague 2, Czech Republic; Department of Analytical Chemistry, Faculty of Science, Charles University in Prague, Albertov 6, 128 43 Prague 2, Czech Republic.
authors.forename: Radomír
authors.initials: R
authors.lastname: Čabala
mesh_terms: D000085:Acetates / Q000097:blood / Q000652:urine; D000138:Acidosis / Q000175:diagnosis*; D000435:Alcoholic Intoxication / Q000097:blood* / Q000652:urine*; D001774:Blood Chemical Analysis / Q000379:methods; D002138:Calibration; D002623:Chemistry Techniques, Analytical; D005260:Female; D005561:Formates / Q000097:blood / Q000652:urine; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006016:Glycolates / Q000097:blood / Q000652:urine; D006801:Humans; D057230:Limit of Detection; D008297:Male; D015203:Reproducibility of Results; D014116:Toxicology / Q000379:methods*; D016482:Urinalysis / Q000379:methods
chemical_list: D000085:Acetates; D005561:Formates; D006016:Glycolates; C031149:glycolic acid; C030544:formic acid; C018968:beta-hydroxyethoxyacetic acid; C034096:ethoxyacetic acid; C013598:methoxyacetic acid
keywords: Alcohol intoxication; Chloroformate; GC–MS; Metabolic acidosis; Organic acid