title: Interfacing low-energy SAW nebulization with Liquid Chromatography-Mass Spectrometry for the analysis of biological samples.
abstract: Soft ionization methods for the introduction of labile biomolecules into a mass spectrometer are of fundamental importance to biomolecular analysis. Previously, electrospray ionization (ESI) and matrix assisted laser desorption-ionization (MALDI) have been the main ionization methods used. Surface acoustic wave nebulization (SAWN) is a new technique that has been demonstrated to deposit less energy into ions upon ion formation and transfer for detection than other methods for sample introduction into a mass spectrometer (MS). Here we report the optimization and use of SAWN as a nebulization technique for the introduction of samples from a low flow of liquid, and the interfacing of SAWN with liquid chromatographic separation (LC) for the analysis of a protein digest. This demonstrates that SAWN can be a viable, low-energy alternative to ESI for the LC-MS analysis of proteomic samples.
authors.affiliation: School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham, UK. B4 7ET.
authors.forename: Karina
authors.initials: K
authors.lastname: Tveen-Jensen
authors.affiliation: Division of Biomedical Engineering, University of Glasgow, Oakfield Avenue, Glasgow, UK. G12 8LT.
authors.forename: Frank
authors.initials: F
authors.lastname: Gesellchen
authors.affiliation: Division of Biomedical Engineering, University of Glasgow, Oakfield Avenue, Glasgow, UK. G12 8LT.
authors.forename: Rab
authors.initials: R
authors.lastname: Wilson
authors.affiliation: School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham, UK. B4 7ET.
authors.forename: Corinne M
authors.initials: CM
authors.lastname: Spickett
authors.affiliation: Division of Biomedical Engineering, University of Glasgow, Oakfield Avenue, Glasgow, UK. G12 8LT.
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Cooper
authors.affiliation: School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham, UK. B4 7ET.
authors.forename: Andrew R
authors.initials: AR
authors.lastname: Pitt
mesh_terms: D001688:Biological Products / Q000737:chemistry*; D002853:Chromatography, Liquid; D006801:Humans; D013058:Mass Spectrometry; D010455:Peptides / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D040901:Proteomics / Q000379:methods
chemical_list: D001688:Biological Products; D010455:Peptides; D011506:Proteins