title: Differing roles of pyruvate dehydrogenase kinases during mouse oocyte maturation.
abstract: Pyruvate dehydrogenase kinases (PDKs) modulate energy homeostasis in multiple tissues and cell types, under various nutrient conditions, through phosphorylation of the α subunit (PDHE1α, also known as PDHA1) of the pyruvate dehydrogenase (PDH) complex. However, the roles of PDKs in meiotic maturation are currently unknown. Here, by undertaking knockdown and overexpression analysis of PDK paralogs (PDK1-PDK4) in mouse oocytes, we established the site-specificity of PDKs towards the phosphorylation of three serine residues (Ser232, Ser293 and Ser300) on PDHE1α. We found that PDK3-mediated phosphorylation of Ser293-PDHE1α results in disruption of meiotic spindle morphology and chromosome alignment and decreased total ATP levels, probably through inhibition of PDH activity. Unexpectedly, we discovered that PDK1 and PDK2 promote meiotic maturation, as their knockdown disturbs the assembly of the meiotic apparatus, without significantly altering ATP content. Moreover, phosphorylation of Ser232-PDHE1α was demonstrated to mediate PDK1 and PDK2 action in meiotic maturation, possibly through a mechanism that is distinct from PDH inactivation. These findings reveal that there are divergent roles of PDKs during oocyte maturation and indicate a new mechanism controlling meiotic structure.
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Hou
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 210029, China College of Animal Science & Technology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Longsen
authors.initials: L
authors.lastname: Han
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Ge
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 210029, China College of Animal Science & Technology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Rujun
authors.initials: R
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Xuesen
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and Gynecology, Washington University School of Medicine, St Louis, MO 63110, USA.
authors.forename: Kelle
authors.initials: K
authors.lastname: Moley
authors.affiliation: Department of Genetics, Washington University School of Medicine, St Louis, MO 63110, USA.
authors.forename: Tim
authors.initials: T
authors.lastname: Schedl
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 210029, China qwang2012@njmu.edu.cn.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D033481:Chromosomes, Mammalian / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D055785:Gene Knockdown Techniques; D008540:Meiosis; D008813:Mice, Inbred ICR; D008954:Models, Biological; D009865:Oocytes / Q000166:cytology* / Q000201:enzymology*; D010766:Phosphorylation; D010768:Phosphoserine / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D000081382:Pyruvate Dehydrogenase Acetyl-Transferring Kinase; D008941:Spindle Apparatus / Q000378:metabolism
chemical_list: C000631433:Pdk1 protein, mouse; C000631435:Pdk2 protein, mouse; C000631437:Pdk3 protein, mouse; C000631439:Pdk4 protein, mouse; D000081382:Pyruvate Dehydrogenase Acetyl-Transferring Kinase; D010768:Phosphoserine; D017346:Protein Serine-Threonine Kinases
keywords: Meiosis; Metabolism; Oocyte; Pyruvate dehydrogenase kinase; Spindle