title: PRPS2 Expression Correlates with Sertoli-Cell Only Syndrome and Inhibits the Apoptosis of TM4 Sertoli Cells.
abstract: OBJECTIVE
  Sertoli-cell only syndrome is one of the reasons for male infertility but its pathogenesis remains unclear. PRPS2, a subset of PRS, is reported to be a potential protein associated with Sertoli-cell only syndrome. In this study we further investigated the correlation between PRPS2 and Sertoli-cell only syndrome, and evaluated the effect of PRPS2 expression on apoptosis of TM4 Sertoli cells.
  
  
  METHODS
  PRPS2 expression was detected in patients with Sertoli-cell only syndrome and normal spermatogenesis, and in Sertoli-cell only syndrome mouse models by immunohistochemistry, quantitative reverse transcription-polymerase chain reaction and Western blot. PRPS2 expression in TM4 Sertoli cells was then down-regulated and up-regulated by lentivirus vectors. The effect of PRPS2 expression on cell apoptosis and cell cycle transition was evaluated by flow cytometry.
  
  
  RESULTS
  PRPS2 expression in patients with Sertoli-cell only syndrome was significantly greater than in those with normal spermatogenesis. A significant increase in PRPS2 expression was observed in Sertoli-cell only syndrome mouse models. PRPS2 over expression significantly inhibited cell apoptosis and promoted cell cycle transition in TM4 Sertoli cells. However, PRPS2 down-regulation showed a reverse effect. Moreover, results revealed that PRPS2 over expression inhibited cell apoptosis via the p53/Bcl-2/caspase-9/caspase-3/caspase-6/caspase-7 signaling pathway.
  
  
  CONCLUSIONS
  PRPS2 expression correlates with Sertoli-cell only syndrome and inhibits the apoptosis of TM4 Sertoli cells via the p53/Bcl-2/caspases signaling pathway.
authors.affiliation: Department of Urology, Peking University Shenzhen Hospital, Shenzhen, People's Republic of China; Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Lei
authors.affiliation: Department of Urology, Peking University Shenzhen Hospital, Shenzhen, People's Republic of China; Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wan
authors.affiliation: Department of Urology, Peking University Shenzhen Hospital, Shenzhen, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Peng
authors.affiliation: Department of Urology, Peking University Shenzhen Hospital, Shenzhen, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Daojun
authors.initials: D
authors.lastname: Lv
authors.affiliation: Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Xuming
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Fangpeng
authors.initials: F
authors.lastname: Shu
authors.affiliation: Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Liren
authors.initials: L
authors.lastname: Zhong
authors.affiliation: Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Huayan
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Urology, Peking University Shenzhen Hospital, Shenzhen, People's Republic of China; Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China. Electronic address: mxming2014@163.com.
authors.forename: Xiangming
authors.initials: X
authors.lastname: Mao
mesh_terms: D000328:Adult; D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D015153:Blotting, Western; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D012313:RNA / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012268:Ribose-Phosphate Pyrophosphokinase / Q000096:biosynthesis / Q000235:genetics*; D054331:Sertoli Cell-Only Syndrome / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D012708:Sertoli Cells / Q000378:metabolism / Q000473:pathology*; D055815:Young Adult
chemical_list: D012313:RNA; C000600435:PRPS2 protein, human; D012268:Ribose-Phosphate Pyrophosphokinase
keywords: Sertoli cell-only syndrome; infertility; male; p53; phosphoribosylpyrophosphate synthetase superactivity; testis