title: Limited Addition of the 6-Arm β1,2-linked N-Acetylglucosamine (GlcNAc) Residue Facilitates the Formation of the Largest N-Glycan in Plants.
abstract: The most abundant N-glycan in plants is the paucimannosidic N-glycan with core β1,2-xylose and α1,3-fucose residues (Man3XylFuc(GlcNAc)2). Here, we report a mechanism in Arabidopsis thaliana that efficiently produces the largest N-glycan in plants. Genetic and biochemical evidence indicates that the addition of the 6-arm β1,2-GlcNAc residue by N-acetylglucosaminyltransferase II (GnTII) is less effective than additions of the core β1,2-xylose and α1,3-fucose residues by XylT, FucTA, and FucTB in Arabidopsis. Furthermore, analysis of gnt2 mutant and 35S:GnTII transgenic plants shows that the addition of the 6-arm non-reducing GlcNAc residue to the common N-glycan acceptor GlcNAcMan3(GlcNAc)2 inhibits additions of the core β1,2-xylose and α1,3-fucose residues. Our findings indicate that plants limit the rate of the addition of the 6-arm GlcNAc residue to the common N-glycan acceptor as a mechanism to facilitate formation of the prevalent N-glycans with Man3XylFuc(GlcNAc)2 and (GlcNAc)2Man3XylFuc(GlcNAc)2 structures.
authors.affiliation: From the Division of Applied Life Science and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, 501 Jinju-daero, Jinju 660-701, Korea.
authors.forename: Jae Yong
authors.initials: JY
authors.lastname: Yoo
authors.affiliation: From the Division of Applied Life Science and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, 501 Jinju-daero, Jinju 660-701, Korea.
authors.forename: Ki Seong
authors.initials: KS
authors.lastname: Ko
authors.affiliation: Department of Chemistry, Changwon National University, 9-Sarim, Changwon 641-773, Korea, and.
authors.forename: Hyun-Kyeong
authors.initials: HK
authors.lastname: Seo
authors.affiliation: Department of Chemistry, Changwon National University, 9-Sarim, Changwon 641-773, Korea, and.
authors.forename: Seongha
authors.initials: S
authors.lastname: Park
authors.affiliation: From the Division of Applied Life Science and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, 501 Jinju-daero, Jinju 660-701, Korea.
authors.forename: Wahyu Indra Duwi
authors.initials: WI
authors.lastname: Fanata
authors.affiliation: From the Division of Applied Life Science and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, 501 Jinju-daero, Jinju 660-701, Korea.
authors.forename: Rikno
authors.initials: R
authors.lastname: Harmoko
authors.affiliation: From the Division of Applied Life Science and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, 501 Jinju-daero, Jinju 660-701, Korea.
authors.forename: Nirmal Kumar
authors.initials: NK
authors.lastname: Ramasamy
authors.affiliation: From the Division of Applied Life Science and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, 501 Jinju-daero, Jinju 660-701, Korea.
authors.forename: Thiyagarajan
authors.initials: T
authors.lastname: Thulasinathan
authors.affiliation: Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana 47907.
authors.forename: Tesfaye
authors.initials: T
authors.lastname: Mengiste
authors.affiliation: Department of Chemistry, Changwon National University, 9-Sarim, Changwon 641-773, Korea, and.
authors.forename: Jae-Min
authors.initials: JM
authors.lastname: Lim
authors.affiliation: From the Division of Applied Life Science and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, 501 Jinju-daero, Jinju 660-701, Korea.
authors.forename: Sang Yeol
authors.initials: SY
authors.lastname: Lee
authors.affiliation: From the Division of Applied Life Science and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, 501 Jinju-daero, Jinju 660-701, Korea, leeko@gnu.ac.kr.
authors.forename: Kyun Oh
authors.initials: KO
authors.lastname: Lee
mesh_terms: D000117:Acetylglucosamine / Q000378:metabolism*; D017360:Arabidopsis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002240:Carbohydrate Sequence; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000096:biosynthesis* / Q000737:chemistry
chemical_list: D011134:Polysaccharides; D000117:Acetylglucosamine
keywords: carbohydrate processing; glycosylation; glycosyltransferase; plant; post-translational modification (PTM)