title: Effect of hyperoxic and hyperbaric conditions on the adenosinergic pathway and CD26 expression in rat.
abstract: The nucleoside adenosine acts on the nervous and cardiovascular systems via the A2A receptor (A2AR). In response to oxygen level in tissues, adenosine plasma concentration is regulated in particular via its synthesis by CD73 and via its degradation by adenosine deaminase (ADA). The cell-surface endopeptidase CD26 controls the concentration of vasoactive and antioxidant peptides and hence regulates the oxygen supply to tissues and oxidative stress response. Although overexpression of adenosine, CD73, ADA, A2AR, and CD26 in response to hypoxia is well documented, the effects of hyperoxic and hyperbaric conditions on these elements deserve further consideration. Rats and a murine Chem-3 cell line that expresses A2AR were exposed to 0.21 bar O2, 0.79 bar N2 (terrestrial conditions; normoxia); 1 bar O2 (hyperoxia); 2 bar O2 (hyperbaric hyperoxia); 0.21 bar O2, 1.79 bar N2 (hyperbaria). Adenosine plasma concentration, CD73, ADA, A2AR expression, and CD26 activity were addressed in vivo, and cAMP production was addressed in cellulo. For in vivo conditions, 1) hyperoxia decreased adenosine plasma level and T cell surface CD26 activity, whereas it increased CD73 expression and ADA level; 2) hyperbaric hyperoxia tended to amplify the trend; and 3) hyperbaria alone lacked significant influence on these parameters. In the brain and in cellulo, 1) hyperoxia decreased A2AR expression; 2) hyperbaric hyperoxia amplified the trend; and 3) hyperbaria alone exhibited the strongest effect. We found a similar pattern regarding both A2AR mRNA synthesis in the brain and cAMP production in Chem-3 cells. Thus a high oxygen level tended to downregulate the adenosinergic pathway and CD26 activity. Hyperbaria alone affected only A2AR expression and cAMP production. We discuss how such mechanisms triggered by hyperoxygenation can limit, through vasoconstriction, the oxygen supply to tissues and the production of reactive oxygen species.
authors.affiliation: UMR MD2, Institute of Biological Research, French Defense Ministry (IRBA), Aix Marseille University, Marseille, France;
authors.forename: Laurie
authors.initials: L
authors.lastname: Bruzzese
authors.affiliation: UMR MD2, Institute of Biological Research, French Defense Ministry (IRBA), Aix Marseille University, Marseille, France;
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Rostain
authors.affiliation: UMR MD2, Institute of Biological Research, French Defense Ministry (IRBA), Aix Marseille University, Marseille, France; Department of Anesthesia and Intensive Care, Timone University Hospital, Marseille, France;
authors.forename: Laëtitia
authors.initials: L
authors.lastname: Née
authors.affiliation: UMR MD2, Institute of Biological Research, French Defense Ministry (IRBA), Aix Marseille University, Marseille, France;
authors.forename: Jocelyne
authors.initials: J
authors.lastname: Condo
authors.affiliation: UMR MD2, Institute of Biological Research, French Defense Ministry (IRBA), Aix Marseille University, Marseille, France; Laboratory of Biochemistry, Timone University Hospital, Marseille, France;
authors.forename: Giovanna
authors.initials: G
authors.lastname: Mottola
authors.affiliation: UMR MD2, Institute of Biological Research, French Defense Ministry (IRBA), Aix Marseille University, Marseille, France;
authors.forename: Nabil
authors.initials: N
authors.lastname: Adjriou
authors.affiliation: Laboratory of Molecular Biology, Conception Hospital, Marseille, France;
authors.forename: Laurence
authors.initials: L
authors.lastname: Mercier
authors.affiliation: Laboratory of Molecular Biology, Conception Hospital, Marseille, France;
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Berge-Lefranc
authors.affiliation: UMR MD2, Institute of Biological Research, French Defense Ministry (IRBA), Aix Marseille University, Marseille, France; Laboratory of Biochemistry, Timone University Hospital, Marseille, France;
authors.forename: Julien
authors.initials: J
authors.lastname: Fromonot
authors.affiliation: UMR MD2, Institute of Biological Research, French Defense Ministry (IRBA), Aix Marseille University, Marseille, France;
authors.forename: Nathalie
authors.initials: N
authors.lastname: Kipson
authors.affiliation: UMRT24, French Institute of Science and Technology for Transport, Development, and Networks (IFSTTAR), Aix Marseille University, Marseille, France;
authors.forename: Michel
authors.initials: M
authors.lastname: Lucciano
authors.affiliation: UMR MD2, Institute of Biological Research, French Defense Ministry (IRBA), Aix Marseille University, Marseille, France;
authors.forename: Josée-Martine
authors.initials: JM
authors.lastname: Durand-Gorde
authors.affiliation: UMR MD2, Institute of Biological Research, French Defense Ministry (IRBA), Aix Marseille University, Marseille, France;
authors.forename: Yves
authors.initials: Y
authors.lastname: Jammes
authors.affiliation: UMR MD2, Institute of Biological Research, French Defense Ministry (IRBA), Aix Marseille University, Marseille, France; Laboratory of Biochemistry, Timone University Hospital, Marseille, France;
authors.forename: Régis
authors.initials: R
authors.lastname: Guieu
authors.affiliation: UMR MD2, Institute of Biological Research, French Defense Ministry (IRBA), Aix Marseille University, Marseille, France; National Institute of Health and Medical Research (INSERM), Paris France;
authors.forename: Jean
authors.initials: J
authors.lastname: Ruf
authors.affiliation: UMR MD2, Institute of Biological Research, French Defense Ministry (IRBA), Aix Marseille University, Marseille, France; National Center of Scientific Research (CNRS), Institute of Biological Science, Paris, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Fenouillet
mesh_terms: D015720:5'-Nucleotidase / Q000235:genetics; D000241:Adenosine / Q000235:genetics*; D000243:Adenosine Deaminase / Q000235:genetics; D000818:Animals; D002460:Cell Line; D000242:Cyclic AMP / Q000235:genetics; D018819:Dipeptidyl Peptidase 4 / Q000235:genetics*; D015536:Down-Regulation / Q000235:genetics; D018496:Hyperoxia / Q000235:genetics*; D008297:Male; D051379:Mice; D018384:Oxidative Stress / Q000235:genetics; D010100:Oxygen / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D043705:Receptor, Adenosine A2A / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D043705:Receptor, Adenosine A2A; D000242:Cyclic AMP; D015720:5'-Nucleotidase; D018819:Dipeptidyl Peptidase 4; D000243:Adenosine Deaminase; D000241:Adenosine; D010100:Oxygen
keywords: CD26 peptidase; adenosine; adenosine receptor; hyperbaric conditions; hyperoxia