title: Indian Hedgehog signaling pathway members are associated with magnetic resonance imaging manifestations and pathological scores in lumbar facet joint osteoarthritis.
abstract: Indian Hedgehog (HH) has been shown to be involved in osteoarthritis (OA) in articular joints, where there is evidence that Indian HH blockade could ameliorate OA. It seems to play a prominent role in development of the intervertebral disc (IVD) and in postnatal maintenance. There is little work on IHH in the IVD. Hence the aim of the current study was to investigate the role of Indian Hedgehog in the pathology of facet joint (FJ) OA. 24 patients diagnosed with lumbar intervertebral disk herniation or degenerative spinal stenosis were included. Preoperative magnetic resonance imaging (MRI) and Osteoarthritis Research Society International (OARSI) histopathology grading system was correlated to the mRNA levels of GLI1, PTCH1, and HHIP in the FJs. The Weishaupt grading and OARSI scores showed high positive correlation (r = 0.894) (P < 0.01). MRI Weishaupt grades showed positive correlation with GLI1 (r = 0.491), PTCH1 (r = 0.444), and HHIP (r = 0.654) mRNA levels (P < 0.05 in each case). OARSI scores were also positively correlated with GLI1 (r = 0. 646), PTCH1 (r = 0. 518), and HHIP (r = 0.762) mRNA levels (P < 0.01 in each case). Cumulatively our findings indicate that Indian HH signaling is increased in OA and is perhaps a key component in OA pathogenesis and progression.
authors.affiliation: 1] Department of Orthopedics, The First Affiliated Hospital of General Hospital of Chinese PLA, Beijing 100048, China [2] Department of Orthopedics, The 94th Hospital of Chinese PLA, Nanchang 330002, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Shuang
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of General Hospital of Chinese PLA, Beijing 100048, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of General Hospital of Chinese PLA, Beijing 100048, China.
authors.forename: Shu-Xun
authors.initials: SX
authors.lastname: Hou
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of General Hospital of Chinese PLA, Beijing 100048, China.
authors.forename: Jia-Liang
authors.initials: JL
authors.lastname: Zhu
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of General Hospital of Chinese PLA, Beijing 100048, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of General Hospital of Chinese PLA, Beijing 100048, China.
authors.forename: Chun-Li
authors.initials: CL
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of General Hospital of Chinese PLA, Beijing 100048, China.
authors.forename: Jia-Guang
authors.initials: JG
authors.lastname: Tang
mesh_terms: D000328:Adult; D000368:Aged; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D005260:Female; D053823:Hedgehog Proteins / Q000378:metabolism*; D006801:Humans; D007403:Intervertebral Disc / Q000473:pathology; D008279:Magnetic Resonance Imaging; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism; D008875:Middle Aged; D010003:Osteoarthritis / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology*; D000072079:Patched Receptors; D000072081:Patched-1 Receptor; D012333:RNA, Messenger / Q000378:metabolism; D011859:Radiography; D060888:Real-Time Polymerase Chain Reaction; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D012720:Severity of Illness Index; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D000071676:Zinc Finger Protein GLI1; D021801:Zygapophyseal Joint / Q000000981:diagnostic imaging / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; C400551:GLI1 protein, human; C117596:HHIP protein, human; D053823:Hedgehog Proteins; D008562:Membrane Glycoproteins; C000606236:PTCH1 protein, human; D000072079:Patched Receptors; D000072081:Patched-1 Receptor; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D014157:Transcription Factors; D000071676:Zinc Finger Protein GLI1