title: A near-infrared fluorescent probe for the selective detection of HNO in living cells and in vivo.
abstract: Nitroxyl (HNO), the one-electron reduced and protonated analogue of nitric oxide (NO), demonstrates distinctive bio-pharmacological effects in the treatment of cardiovascular disorders. Herein, we design and synthesize a near-infrared (NIR) metal-free fluorescent probe Cyto-JN for the detection of nitroxyl (HNO) in living cells and in vivo. The metal-free Cyto-JN is composed of two moieties: the Aza-BODIPY fluorophore and the HNO recognition unit, the diphenylphosphinobenzoyl group. Cyto-JN can react with HNO in a 1 : 1 stoichiometry, which may bring great benefit to the detection efficiency of bioassays. Cyto-JN shows high sensitivity toward HNO and exhibits low cytotoxic effect on cells. Moreover, the probe displays good selectivity for the detection of HNO in the presence of various biologically related species. Cyto-JN can be applied successfully to bio-imaging of HNO in living cells and in mice. The results of flow cytometry confirm that the probe Cyto-JN can be used to detect intracellular HNO qualitatively and quantitatively.
authors.affiliation: Key Laboratory of Coastal Environmental Processes and Ecological Remediation; The Research Center for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China. fbyu@yic.ac.cn lxchen@yic.ac.cn.
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.forename: Xiaotong
authors.initials: X
authors.lastname: Jing
authors.forename: Fabiao
authors.initials: F
authors.lastname: Yu
authors.forename: Changjun
authors.initials: C
authors.lastname: Lv
authors.forename: Lingxin
authors.initials: L
authors.lastname: Chen
mesh_terms: D000818:Animals; D002470:Cell Survival; D015195:Drug Design; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D007259:Infrared Rays; D051379:Mice; D057054:Molecular Imaging; D009589:Nitrogen Oxides / Q000032:analysis* / Q000737:chemistry; D000067996:RAW 264.7 Cells
chemical_list: D005456:Fluorescent Dyes; D009589:Nitrogen Oxides; C039900:nitroxyl