title: Antimicrobial activity and cytotoxicity of nanofibrous mats immobilized with polysaccharides-rectorite based nanogels.
abstract: Rectorite (REC)-encapsulated lysozyme (LY)-alginate (ALG) nanogels (NGs) were prepared by adding ALG-REC composites suspensions into LY solutions at the mass ratio of 1:2. The morphology of the NGs and the NGs-assembled nanofibrous mats were studied by transmission electron microscope and field emission scanning electron microscopy, respectively. The composition of NGs-immobilized nanofibrous mats was detected by X-ray photoelectron spectroscopy. The NGs-assembled nanofibrous mats with the addition of REC could enhance the inhibition against Escherichia coli and Staphylococcus aureus. Additionally, NGs-coated mats reduced the toxicity of cellulose mats on mouse lung fibroblasts using MTT assay. Besides, the addition of REC in the NGs improved the cell compatibility of NGs-assembled nanofibrous mats.
authors.affiliation: Department of Environmental Science, Hubei Key Lab of Biomass Resource and Environmental Biotechnology, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Plastic Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Xueyong
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Food Science and Technology, Huazhong Agricultural University, No. 1 Shizishan Road, Wuhan 430070, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Tian
authors.affiliation: School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Environmental Science, Hubei Key Lab of Biomass Resource and Environmental Biotechnology, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China.
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Shi
authors.affiliation: Department of Environmental Science, Hubei Key Lab of Biomass Resource and Environmental Biotechnology, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China.
authors.forename: Yingfei
authors.initials: Y
authors.lastname: Zhan
authors.affiliation: Department of Environmental Science, Hubei Key Lab of Biomass Resource and Environmental Biotechnology, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China.
authors.forename: Yumin
authors.initials: Y
authors.lastname: Du
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China. Electronic address: liuhuan@wh.iov.cn.
authors.forename: Huan
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Environmental Science, Hubei Key Lab of Biomass Resource and Environmental Biotechnology, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China. Electronic address: hbdeng@whu.edu.cn.
authors.forename: Hongbing
authors.initials: H
authors.lastname: Deng
mesh_terms: D000538:Aluminum Silicates / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D004926:Escherichia coli / Q000187:drug effects; D005782:Gels; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D008903:Minerals / Q000737:chemistry*; D057139:Nanofibers; D011134:Polysaccharides / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000538:Aluminum Silicates; D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D005782:Gels; D008903:Minerals; D011134:Polysaccharides; C546376:rectorite
keywords: Antibacterial; Cytotoxicity; Nanogels; Rectorite