title: Sarcomeres Pattern Proprioceptive Sensory Dendritic Endings through UNC-52/Perlecan in C. elegans.
abstract: Sensory dendrites innervate peripheral tissues through cell-cell interactions that are poorly understood. The proprioceptive neuron PVD in C. elegans extends regular terminal dendritic branches between muscle and hypodermis. We found that the PVD branch pattern was instructed by adhesion molecule SAX-7/L1CAM, which formed regularly spaced stripes on the hypodermal cell. The regularity of the SAX-7 pattern originated from the repeated and regularly spaced dense body of the sarcomeres in the muscle. The extracellular proteoglycan UNC-52/Perlecan linked the dense body to the hemidesmosome on the hypodermal cells, which in turn instructed the SAX-7 stripes and PVD dendrites. Both UNC-52 and hemidesmosome components exhibited highly regular stripes that interdigitated with the SAX-7 stripe and PVD dendrites, reflecting the striking precision of subcellular patterning between muscle, hypodermis, and dendrites. Hence, the muscular contractile apparatus provides the instructive cues to pattern proprioceptive dendrites.
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Liang
authors.affiliation: Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
authors.forename: Xintong
authors.initials: X
authors.lastname: Dong
authors.affiliation: Department of Zoology, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.
authors.forename: Donald G
authors.initials: DG
authors.lastname: Moerman
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, China; Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA. Electronic address: kangshen@stanford.edu.
authors.forename: Kang
authors.initials: K
authors.lastname: Shen
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, China. Electronic address: xmwang@moon.ibp.ac.cn.
authors.forename: Xiangming
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism*; D003712:Dendrites / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D019812:Heparan Sulfate Proteoglycans / Q000378:metabolism*; D007091:Image Processing, Computer-Assisted; D008565:Membrane Proteins / Q000378:metabolism*; D018613:Microscopy, Confocal; D009132:Muscles / Q000166:cytology / Q000378:metabolism*; D039842:Neural Cell Adhesion Molecule L1 / Q000378:metabolism; D019006:Neural Cell Adhesion Molecules / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D011509:Proteoglycans / Q000378:metabolism*; D012518:Sarcomeres / Q000378:metabolism*; D013347:Subcellular Fractions; D040521:Subcutaneous Tissue / Q000378:metabolism*
chemical_list: D029742:Caenorhabditis elegans Proteins; D019812:Heparan Sulfate Proteoglycans; D008565:Membrane Proteins; D039842:Neural Cell Adhesion Molecule L1; D019006:Neural Cell Adhesion Molecules; D011509:Proteoglycans; C501528:SAX-7 protein, C elegans; C082396:unc-52 protein, C elegans; C071927:perlecan