title: Effect in dedicator of cytokinesis 6 (DOCK6) on steroid production in theca cells of follicular cysts.
abstract: Ovarian follicular cysts are one of the most common causes of reproductive failure in mammals. A comparative gene expression approach may aid in elucidating the causes of ovarian cyst disease. In the present study, the differential display technique was used to identify mRNA sequences that accumulate preferentially in theca cells of bovine cystic follicles. Dedicator of cytokinesis 6 (Dock6) expression was observed in the theca cells of cystic follicles. Small interfering RNA (siRNA) knockdown of Dock6 increased progesterone (P4) production and StAR expression in theca cells of high-estrogen follicular cysts, but did not affect androstenedione (A4) production. We propose that Dock6 may be a marker associated with the development of follicular cysts. Additionally, Dock6 may be involved in the development of cystic follicles by suppressing P4 production rather than increasing A4 production in theca cells.
authors.affiliation: Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Inada-machi, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Chiaki
authors.initials: C
authors.lastname: Murayama
authors.affiliation: Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Inada-machi, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Eiki
authors.initials: E
authors.lastname: Yamasaki
authors.affiliation: Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Inada-machi, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Miyamoto
authors.affiliation: Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Inada-machi, Obihiro, Hokkaido 080-8555, Japan. Electronic address: shimizut@obihiro.ac.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Shimizu
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D017931:DNA Primers; D004967:Estrogens / Q000096:biosynthesis*; D005260:Female; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics / Q000502:physiology*; D006080:Ovarian Follicle / Q000166:cytology / Q000378:metabolism*; D011374:Progesterone / Q000096:biosynthesis*; D034622:RNA Interference; D034741:RNA, Small Interfering; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D004967:Estrogens; D020662:Guanine Nucleotide Exchange Factors; D034741:RNA, Small Interfering; D011374:Progesterone
keywords: Androstenedione; Bovine; Follicular cyst; Progesterone; Theca cell