title: Persistence of the rotavirus A genome in mesenteric lymph nodes of cattle raised on farms.
abstract: Previous studies revealed that rotavirus A (RVA) is present in not only the small intestine but also various organs. It was reported that RVA persisted in mesenteric lymph nodes (MLNs) in experimental models. However, there have been no reports focused on RVA in MLNs of animals under natural conditions. In this study, in order to investigate the persistence of the RVA genome in MLNs in cattle under natural conditions, reverse transcription-semi-nested PCR was carried out to detect RVA genomes in the MLNs from 17 calves that had been subjected to autopsy examinations. RVA genomes were detected in MLNs from 10 (˜60  %) of the 17 autopsied calves. MLNs from 170 healthy adult cattle that had been slaughtered were also examined; 15 (∼10  %) of the 170 cattle had RVA genomes in their MLNs, indicating that RNA genomes are found frequently in MLNs of cattle under natural conditions. Genetic analyses revealed that RVAs in MLNs were classified as G and/or P genotypes generally prevalent in bovines. Basically, the strains in intestinal contents were genetically identical to those in MLNs from individual cattle, suggesting that bovine RVAs have the ability to spread from the intestine to MLNs. Furthermore, amongst RVA-positive cattle, six of 10 autopsied calves and 12 of 15 healthy adult cattle were negative for the virus in the intestinal contents, indicating that bovine RVA genomes can persist in MLNs after viral clearance in the digestive tract.
authors.affiliation: The United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Mitake
authors.affiliation: The United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.|Laboratory of Zoonotic Diseases, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors.forename: Naoto
authors.initials: N
authors.lastname: Ito
authors.affiliation: The United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors.forename: Kota
authors.initials: K
authors.lastname: Okadera
authors.affiliation: The United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors.forename: Kazuma
authors.initials: K
authors.lastname: Okada
authors.affiliation: The United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors.forename: Kento
authors.initials: K
authors.lastname: Nakagawa
authors.affiliation: Fukui Prefectural Livestock Hygiene Service Center, 69-10-1 Obatake, Fukui 918-8226, Japan.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Tanaka
authors.affiliation: Fukui Prefectural Livestock Hygiene Service Center, 69-10-1 Obatake, Fukui 918-8226, Japan.
authors.forename: Kiyohito
authors.initials: K
authors.lastname: Katsuragi
authors.affiliation: Fukui Prefectural Livestock Hygiene Service Center, 69-10-1 Obatake, Fukui 918-8226, Japan.
authors.forename: Kasumi
authors.initials: K
authors.lastname: Kasahara
authors.affiliation: Fukui Prefectural Livestock Hygiene Service Center, 69-10-1 Obatake, Fukui 918-8226, Japan.
authors.forename: Toshihide
authors.initials: T
authors.lastname: Nihongi
authors.affiliation: Gifu City Meat Hygiene Inspection Center, 5-148 Sakaigawa, Gifu 500-8226, Japan.
authors.forename: Shoji
authors.initials: S
authors.lastname: Sakurai
authors.affiliation: The United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.|Dairy Hygiene Research Division, National Institute of Animal Health, 4 Hitsujigaoka, Toyohira, Sapporo, Hokkaido 062-0045, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tsunemitsu
authors.affiliation: The United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.|Laboratory of Zoonotic Diseases, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Sugiyama
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D005260:Female; D016679:Genome, Viral; D005838:Genotype; D008198:Lymph Nodes / Q000821:virology*; D008297:Male; D012401:Rotavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000662:veterinary* / Q000821:virology