title: Characterization of Cellobiose Dehydrogenase from a Biotechnologically Important Cerrena unicolor Strain.
abstract: Cellobiose dehydrogenase (CDH), a secreted flavocytochrome produced by a number of wood-degrading fungi, was detected in the culture supernatant of a biotechnologically important strain of Cerrena unicolor grown in a modified cellulose-based liquid medium. The enzyme was purified as two active fractions: CuCDH-FAD (flavin domain) (1.51-fold) with recovery of 8.35 % and CuCDH (flavo-heme enzyme) (21.21-fold) with recovery of 73.41 %. As CDH from other wood-rotting fungi, the intact form of cellobiose dehydrogenase of C. unicolor is a monomeric protein containing one flavin and one heme b with molecular mass 97 kDa and pI = 4.55. The enzyme is glycosylated (8.2 %) mainly with mannose and glucosamine residues. Moreover, the cellobiose dehydrogenase gene cdh1 and its corresponding cDNA from the fungus C. unicolor were isolated, cloned, and characterized. The 2316-bp full-length cDNA of cdh1 encoded a mature CDH protein containing 771 amino acids preceded by a signal peptide consisting of 18 amino acids. Moreover, both active fractions were characterized in terms of kinetics, temperature and pH optima, and antioxidant properties.
authors.affiliation: Department of Biochemistry, Maria Curie-Skłodowska University, Akademicka 19 St., 20-033, Lublin, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Sulej
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Janusz
authors.forename: Monika
authors.initials: M
authors.lastname: Osińska-Jaroszuk
authors.forename: Patrycja
authors.initials: P
authors.lastname: Rachubik
authors.forename: Andrzej
authors.initials: A
authors.lastname: Mazur
authors.forename: Iwona
authors.initials: I
authors.lastname: Komaniecka
authors.forename: Adam
authors.initials: A
authors.lastname: Choma
authors.forename: Jerzy
authors.initials: J
authors.lastname: Rogalski
mesh_terms: D002237:Carbohydrate Dehydrogenases / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics; D002482:Cellulose / Q000737:chemistry* / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics; D020072:Polyporales / Q000201:enzymology* / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D005656:Fungal Proteins; D002482:Cellulose; D002237:Carbohydrate Dehydrogenases; C019859:cellobiose-quinone oxidoreductase