title: Light up ClO(-) in live cells using an aza-coumarin based fluorescent probe with fast response and high sensitivity.
abstract: Hypochlorous acid (HClO)/hypochlorite (ClO(-)), one of the reactive oxygen species (ROS), is a key microbicidal agent used for natural defense; however, HClO is also responsible for some human diseases. Although much effort has been made to develop HClO-selective fluorescent probes, many of them display a delayed response time and nanomole-sensitive probes are rare. In this study, we designed and synthesized an aza-coumarin based fluorescent probe AC-ClO for ClO(-) determination with fast response (completed within 2 min) and high sensitivity (detection limit is 25 nM). AC-ClO displayed a color change from pink to light yellow and a remarkable "turn-on" fluorescence response towards ClO(-). Confocal fluorescence microscopy experiments demonstrated that the probe could be applied for the live-cell imaging of exogenous and endogenous ClO(-).
authors.affiliation: State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian, China. fanjl@dlut.edu.cn.
authors.forename: Jiangli
authors.initials: J
authors.lastname: Fan
authors.forename: Huiying
authors.initials: H
authors.lastname: Mu
authors.forename: Hao
authors.initials: H
authors.lastname: Zhu
authors.forename: Jingyun
authors.initials: J
authors.lastname: Wang
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Peng
mesh_terms: D000818:Animals; D001372:Aza Compounds / Q000737:chemistry*; D002470:Cell Survival; D003116:Color; D003374:Coumarins / Q000737:chemistry*; D015195:Drug Design; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D006997:Hypochlorous Acid / Q000032:analysis* / Q000737:chemistry; D057230:Limit of Detection; D061986:MCF-7 Cells; D051379:Mice; D008856:Microscopy, Fluorescence; D000067996:RAW 264.7 Cells; D013997:Time Factors
chemical_list: D001372:Aza Compounds; D003374:Coumarins; D005456:Fluorescent Dyes; D006997:Hypochlorous Acid; C030123:coumarin