title: Patatin-Like Phospholipase Domain-Containing 3 I148M Variant Is Associated with Liver Steatosis and Fat Distribution in Chronic Hepatitis B.
abstract: BACKGROUND
  The patatin-like phospholipase domain-containing 3 gene (PNPLA3) has been associated with liver steatosis and disease progression in nonalcoholic steatohepatitis and chronic hepatitis C.
  
  
  OBJECTIVE
  The aim of the present study was to evaluate the influence of the PNPLA3 I148M polymorphisms on the clinical, histological, viral, and host parameters in Italian patients with chronic hepatitis B (CHB).
  
  
  METHODS
  Ninety-nine patients with CHB entered the study and underwent a clinical, histological, virological, and biochemical evaluation. PNPLA3 (p.I148M) variants were genotyped.
  
  
  RESULTS
  PNPLA3 rare variant (148M) was significantly associated with liver steatosis (p = 0.0019) and cholesterol (p = 0.04) levels, but not with fibrosis or histological activity index. The 13 patients with severe liver steatosis (score > 3) (38%) were more frequently homozygous for PNPLA3 148M variant than the 86 without (6%, p = 0.003). At logistic regression analysis, severe steatosis was independently associated with the rare allele (p = 0.001) and waist circumference, but not with body mass index (BMI).
  
  
  CONCLUSIONS
  In our CHB patients, the PNPLA3 polymorphisms influenced the development of liver steatosis, but not fibrosis status. The association of PNPLA3 p.I148M with liver steatosis increased with the greater amount of abdominal fat, irrespective of BMI.
authors.affiliation: Internal Medicine and Hepatology, Second University of Naples, Via Pansini, 5-Edificio 3, 80131, Naples, Italy. rosa.zampino@unina2.it.
authors.forename: Rosa
authors.initials: R
authors.lastname: Zampino
authors.affiliation: Department of Public Medicine, Section of Infectious Diseases, Second University of Naples, Naples, Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Coppola
authors.affiliation: Department of Pediatrics, Second University of Naples, Naples, Italy.
authors.forename: Grazia
authors.initials: G
authors.lastname: Cirillo
authors.affiliation: Internal Medicine and Hepatology, Second University of Naples, Via Pansini, 5-Edificio 3, 80131, Naples, Italy.
authors.forename: Adriana
authors.initials: A
authors.lastname: Boemio
authors.affiliation: Department of Pediatrics, Second University of Naples, Naples, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Grandone
authors.affiliation: Department of Clinical and Experimental Medicine and Surgery, Second University of Naples, Naples, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Stanzione
authors.affiliation: Department of Public Medicine, Section of Infectious Diseases, Second University of Naples, Naples, Italy.
authors.forename: Nicolina
authors.initials: N
authors.lastname: Capoluongo
authors.affiliation: Internal Medicine and Hepatology, Second University of Naples, Via Pansini, 5-Edificio 3, 80131, Naples, Italy.
authors.forename: Aldo
authors.initials: A
authors.lastname: Marrone
authors.affiliation: Department of Public Medicine, Section of Infectious Diseases, Second University of Naples, Naples, Italy.
authors.forename: Margherita
authors.initials: M
authors.lastname: Macera
authors.affiliation: Department of Public Medicine, Section of Infectious Diseases, Second University of Naples, Naples, Italy.
authors.forename: Evangelista
authors.initials: E
authors.lastname: Sagnelli
authors.affiliation: Internal Medicine and Hepatology, Second University of Naples, Via Pansini, 5-Edificio 3, 80131, Naples, Italy.
authors.forename: Luigi Elio
authors.initials: LE
authors.lastname: Adinolfi
authors.affiliation: Department of Pediatrics, Second University of Naples, Naples, Italy.
authors.forename: Emanuele Miraglia
authors.initials: EM
authors.lastname: del Giudice
mesh_terms: D050153:Abdominal Fat; D000328:Adult; D000368:Aged; D000886:Anthropometry; D015992:Body Mass Index; D016009:Chi-Square Distribution; D015331:Cohort Studies; D018450:Disease Progression; D005234:Fatty Liver / Q000150:complications / Q000235:genetics* / Q000503:physiopathology; D005260:Female; D020022:Genetic Predisposition to Disease / Q000453:epidemiology*; D019694:Hepatitis B, Chronic / Q000150:complications / Q000235:genetics* / Q000503:physiopathology; D006801:Humans; D007558:Italy; D008049:Lipase / Q000235:genetics*; D016015:Logistic Models; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D008875:Middle Aged; D015999:Multivariate Analysis; D011110:Polymorphism, Genetic; D011446:Prospective Studies; D018570:Risk Assessment; D012720:Severity of Illness Index; D055815:Young Adult; D000217:Acyltransferases; D054512:Phospholipases A2, Calcium-Independent
chemical_list: D008049:Lipase; D008565:Membrane Proteins; C533682:PNPLA3 protein, human; D000217:Acyltransferases; D054512:Phospholipases A2, Calcium-Independent
keywords: Abdominal fat; BMI; HBV; PNPLA3