title: Roles of miRNAs in microcystin-LR-induced Sertoli cell toxicity.
abstract: Microcystin (MC)-LR, a cyclic heptapeptide, is a potent reproductive system toxin. To understand the molecular mechanisms of MC-induced reproductive system cytotoxicity, we evaluated global changes of miRNA and mRNA expression in mouse Sertoli cells following MC-LR treatment. Our results revealed that the exposure to MC-LR resulted in an altered miRNA expression profile that might be responsible for the modulation of mRNA expression. Bio-functional analysis indicated that the altered genes were involved in specific cellular processes, including cell death and proliferation. Target gene analysis suggested that junction injury in Sertoli cells exposed to MC-LR might be mediated by miRNAs through the regulation of the Sertoli cell-Sertoli cell pathway. Collectively, these findings may enhance our understanding on the modes of action of MC-LR on mouse Sertoli cells as well as the molecular mechanisms underlying the toxicity of MC-LR on the male reproductive system.
authors.affiliation: Immunology and Reproduction Biology Laboratory & State Key Laboratory of Analytical Chemistry for Life Science, Medical School, Nanjing University, Nanjing, Jiangsu 210093, PR China; Jiangsu Key Laboratory of Molecular Medicine, Nanjing University, Nanjing, Jiangsu 210093, PR China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: The Centre for Individualized Medication, Linköping University Hospital, Linköping University, Linköping SE-58185, Sweden.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Immunology and Reproduction Biology Laboratory & State Key Laboratory of Analytical Chemistry for Life Science, Medical School, Nanjing University, Nanjing, Jiangsu 210093, PR China; Jiangsu Key Laboratory of Molecular Medicine, Nanjing University, Nanjing, Jiangsu 210093, PR China.
authors.forename: Cong
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Urology, Affiliated Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing 210008, PR China.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Qiu
authors.affiliation: The Centre for Individualized Medication, Linköping University Hospital, Linköping University, Linköping SE-58185, Sweden.
authors.forename: Mikael
authors.initials: M
authors.lastname: Benson
authors.affiliation: Immunology and Reproduction Biology Laboratory & State Key Laboratory of Analytical Chemistry for Life Science, Medical School, Nanjing University, Nanjing, Jiangsu 210093, PR China; Jiangsu Key Laboratory of Molecular Medicine, Nanjing University, Nanjing, Jiangsu 210093, PR China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Yin
authors.affiliation: Department of Microbiology and Immunology, Mucosal Immunobiology and Vaccine Research Center, Institute of Biomedicine, University of Gothenburg, Gothenburg, Sweden.
authors.forename: Zou
authors.initials: Z
authors.lastname: Xiang
authors.affiliation: Immunology and Reproduction Biology Laboratory & State Key Laboratory of Analytical Chemistry for Life Science, Medical School, Nanjing University, Nanjing, Jiangsu 210093, PR China; Jiangsu Key Laboratory of Molecular Medicine, Nanjing University, Nanjing, Jiangsu 210093, PR China. Electronic address: lidm@nju.edu.cn.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Li
authors.affiliation: Immunology and Reproduction Biology Laboratory & State Key Laboratory of Analytical Chemistry for Life Science, Medical School, Nanjing University, Nanjing, Jiangsu 210093, PR China; Jiangsu Key Laboratory of Molecular Medicine, Nanjing University, Nanjing, Jiangsu 210093, PR China. Electronic address: hanxd@nju.edu.cn.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Han
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D019295:Computational Biology; D004305:Dose-Response Relationship, Drug; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D008297:Male; D008387:Marine Toxins; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000378:metabolism*; D052998:Microcystins / Q000633:toxicity*; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D012708:Sertoli Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology
chemical_list: D008387:Marine Toxins; D035683:MicroRNAs; D052998:Microcystins; D012333:RNA, Messenger; C057862:cyanoginosin LR
keywords: Bioinformatics; Microcystin-LR; Sertoli cell; Toxicity; mRNA; microRNA