title: Binding of citreoviridin to human serum albumin: multispectroscopic and molecular docking.
abstract: Citreoviridin (CIT), a mycotoxin produced by Penicillium citreonigrum, is a common contaminant of wide range of agriproducts and detrimental to human and animal health. In this study, the interaction of CIT with human serum albumin (HSA) is researched by steady-state fluorescence, ultraviolet-visible (UV-Vis) absorption, circular dichroism (CD) methods, and molecular modeling. The association constants, binding site numbers, and corresponding thermodynamic parameters are used to investigate the quenching mechanism. The alternations of HSA secondary structure in the presence of CIT are demonstrated with UV-Vis, synchronous fluorescence, and CD spectra. The molecular modeling results reveal that CIT can bind with hydrophobic pocket of HSA with hydrophobic and hydrogen bond force. Moreover, an apparent distance of 3.25 nm between Trp214 and CIT is obtained via fluorescence resonance energy transfer method.
authors.affiliation: School of Public Health, Shandong University, Jinan 250012, China ; School of Public Health, Taishan Medical University, Taian 271016, China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Hou
authors.affiliation: School of Pharmacy, Taishan Medical University, Taian 271016, China.
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Qu
authors.affiliation: School of Pharmacy, Taishan Medical University, Taian 271016, China.
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Pharmacy, Taishan Medical University, Taian 271016, China.
authors.forename: Yueyue
authors.initials: Y
authors.lastname: Kong
authors.affiliation: School of Pharmacy, Taishan Medical University, Taian 271016, China.
authors.forename: Baoxiu
authors.initials: B
authors.lastname: Jia
authors.affiliation: School of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 271000, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Yao
authors.affiliation: School of Public Health, Shandong University, Jinan 250012, China.
authors.forename: Baofa
authors.initials: B
authors.lastname: Jiang
mesh_terms: D001313:Aurovertins / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites; D002942:Circular Dichroism; D006801:Humans; D006860:Hydrogen Bonding; D057927:Hydrophobic and Hydrophilic Interactions; D008958:Models, Molecular; D062105:Molecular Docking Simulation; D010407:Penicillium / Q000737:chemistry / Q000472:pathogenicity; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry* / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics
chemical_list: D001313:Aurovertins; D012709:Serum Albumin; C014416:citreoviridin