title: Light-induced STOMAGEN-mediated stomatal development in Arabidopsis leaves.
abstract: The initiation of stomata, microscopic valves in the epidermis of higher plants that control of gas exchange, requires a co-ordinated sequence of asymmetric and symmetric divisions, which is under tight environmental and developmental control. Arabidopsis leaves grown under elevated photosynthetic photon flux density have a higher density of stomata. STOMAGEN encodes an epidermal patterning factor produced in the mesophyll, and our observations indicated that elevated photosynthetic irradiation stimulates STOMAGEN expression. Our analysis of gain and loss of function of STOMAGEN further detailed its function as a positive regulator of stomatal formation on both sides of the leaf, not only in terms of stomatal density across the leaf surface but also in terms of their stomatal index. STOMAGEN function was rate limiting for the light response of the stomatal lineage in the adaxial epidermis. Mutants in pathways that regulate stomatal spacing in the epidermis and have elevated stomatal density, such as stomatal density and distribution (sdd1) and too many mouth alleles, displayed elevated STOMAGEN expression, suggesting that STOMAGEN is either under the direct control of these pathways or is indirectly affected by stomatal patterning, suggestive of a feedback mechanism. These observations support a model in which changes in levels of light irradiation are perceived in the mesophyll and control the production of stomata in the epidermis by mesophyll-produced STOMAGEN, and whereby, conversely, stomatal patterning, either directly or indirectly, influences STOMAGEN levels.
authors.affiliation: Institute of Plant Molecular Biology, The Biology Centre of the Czech Academy of Sciences, Branisovska 31, 370 05 Ceske Budejovice, Czech Republic Department of Experimental Plant Biology, Faculty of Science, University of South Bohemia, Branisovska 1760, 370 05 Ceske Budejovice, Czech Republic hronkova@umbr.cas.cz.
authors.forename: Marie
authors.initials: M
authors.lastname: Hronková
authors.affiliation: Institute of Plant Molecular Biology, The Biology Centre of the Czech Academy of Sciences, Branisovska 31, 370 05 Ceske Budejovice, Czech Republic.
authors.forename: Dana
authors.initials: D
authors.lastname: Wiesnerová
authors.affiliation: Institute of Plant Molecular Biology, The Biology Centre of the Czech Academy of Sciences, Branisovska 31, 370 05 Ceske Budejovice, Czech Republic.
authors.forename: Marie
authors.initials: M
authors.lastname: Šimková
authors.affiliation: Institute of Experimental Botany, The Czech Academy of Sciences, Rozvojová 263, 165 02 Prague 6, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Skůpa
authors.affiliation: Cardiff School of Biosciences, The Sir Martin Evans Building, Museum Avenue, Cardiff CF10 3AX, UK.
authors.forename: Walter
authors.initials: W
authors.lastname: Dewitte
authors.affiliation: Department of Experimental Plant Biology, Faculty of Science, University of South Bohemia, Branisovska 1760, 370 05 Ceske Budejovice, Czech Republic.
authors.forename: Martina
authors.initials: M
authors.lastname: Vráblová
authors.affiliation: Institute of Plant Molecular Biology, The Biology Centre of the Czech Academy of Sciences, Branisovska 31, 370 05 Ceske Budejovice, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Zažímalová
authors.affiliation: Institute of Plant Molecular Biology, The Biology Centre of the Czech Academy of Sciences, Branisovska 31, 370 05 Ceske Budejovice, Czech Republic Department of Experimental Plant Biology, Faculty of Science, University of South Bohemia, Branisovska 1760, 370 05 Ceske Budejovice, Czech Republic.
authors.forename: Jiří
authors.initials: J
authors.lastname: Šantrůček
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000528:radiation effects; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects*; D008027:Light; D010788:Photosynthesis; D018515:Plant Leaves / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects; D054046:Plant Stomata / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects; D015398:Signal Transduction
chemical_list: D029681:Arabidopsis Proteins; C547254:Stomagen protein, Arabidopsis
keywords: Arabidopsis thaliana; PPFD; SDD1; STOMAGEN; TMM.; stomatal density