title: [Purification of enramycin by macroporous resin adsorption and reversed phase chromatography purification].
abstract: Enramycin is a polypeptide antibiotic and new, safe animal feed additive. A new purification process was developed, based on pre-purification by macroporous resin and refining by reversed phase chromatography. AB-8 macroporous resin was used for the pre-purification process of enramycin, with an elution buffer of 0.012 mol/L aqueous HCl solution-methanol (50: 50, V/V). Then, enramycin a and enramycin b were separated effectively by C18 reversed phase chromatography, with a elution buffer of 0.05 mol/L aqueous KH2PO4 solution-acetonitrile (70: 30, V/V, pH 4.5). The purities of enramycin a and enramycin b were up to 98.5% and 98.0%, respectively. The yield reached 29.2%. This study would provide a useful reference for the preparation of enramycin a and enramycin b with a high purity.
authors.forename: Wu
authors.initials: W
authors.lastname: Jiaxin
authors.forename: Huang
authors.initials: H
authors.lastname: Yongdong
authors.forename: Qi
authors.initials: Q
authors.lastname: Peng
authors.forename: He
authors.initials: H
authors.lastname: Jihong
authors.forename: Li
authors.initials: L
authors.lastname: Ping
authors.forename: Zhang
authors.initials: Z
authors.lastname: Guodong
authors.forename: Zhao
authors.initials: Z
authors.lastname: Meixian
mesh_terms: D000327:Adsorption; D000900:Anti-Bacterial Agents / Q000302:isolation & purification*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D010455:Peptides / Q000302:isolation & purification; D010456:Peptides, Cyclic
chemical_list: D000900:Anti-Bacterial Agents; D010455:Peptides; C044447:enramycin; D010456:Peptides, Cyclic