title: Relation between cell death progression, reactive oxygen species production and mitochondrial membrane potential in fermenting Saccharomyces cerevisiae cells under heat-shock conditions.
abstract: Moderate heat shock increased reactive oxygen species (ROS) production that led to cell death in glucose-grown Saccharomyces cerevisiae cells. Conditions that disturb mitochondrial functions such as treatment by uncouplers and petite mutation were shown to inhibit ROS production and protects cell from thermal death. Hence, mitochondria are responsible for ROS production and play an active role in cell death. An increase in ROS production was accompanied by hyperpolarization of inner mitochondrial membrane. All agents suppressing hyperpolarization also suppressed heat-induced ROS production. It was supposed that generation of ROS under moderate heat shock in glucose-grown S. cerevisiae cells is driven by the mitochondrial membrane potential.
authors.affiliation: Siberian Institute of Plant Physiology and Biochemistry, 132 Lermontov St., Irkutsk, 664033 Russia.
authors.forename: Darya V
authors.initials: DV
authors.lastname: Pyatrikas
authors.affiliation: Siberian Institute of Plant Physiology and Biochemistry, 132 Lermontov St., Irkutsk, 664033 Russia fedoseeva@sifibr.irk.ru.
authors.forename: Irina V
authors.initials: IV
authors.lastname: Fedoseeva
authors.affiliation: Siberian Institute of Plant Physiology and Biochemistry, 132 Lermontov St., Irkutsk, 664033 Russia.
authors.forename: Nina N
authors.initials: NN
authors.lastname: Varakina
authors.affiliation: Siberian Institute of Plant Physiology and Biochemistry, 132 Lermontov St., Irkutsk, 664033 Russia.
authors.forename: Tatyana M
authors.initials: TM
authors.lastname: Rusaleva
authors.affiliation: Siberian Institute of Plant Physiology and Biochemistry, 132 Lermontov St., Irkutsk, 664033 Russia.
authors.forename: Alexei V
authors.initials: AV
authors.lastname: Stepanov
authors.affiliation: Siberian Institute of Plant Physiology and Biochemistry, 132 Lermontov St., Irkutsk, 664033 Russia.
authors.forename: Anna V
authors.initials: AV
authors.lastname: Fedyaeva
authors.affiliation: Siberian Institute of Plant Physiology and Biochemistry, 132 Lermontov St., Irkutsk, 664033 Russia.
authors.forename: Gennadii B
authors.initials: GB
authors.lastname: Borovskii
authors.affiliation: Siberian Institute of Plant Physiology and Biochemistry, 132 Lermontov St., Irkutsk, 664033 Russia.
authors.forename: Eugene G
authors.initials: EG
authors.lastname: Rikhvanov
mesh_terms: D019297:2,4-Dinitrophenol / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D005285:Fermentation; D005800:Genes, Fungal / Q000235:genetics; D005947:Glucose / Q000378:metabolism; D006358:Hot Temperature; D053078:Membrane Potential, Mitochondrial / Q000502:physiology*; D050296:Microbial Viability; D009154:Mutation; D061209:Proton Ionophores / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D061209:Proton Ionophores; D017382:Reactive Oxygen Species; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D005947:Glucose; D001205:Ascorbic Acid; D019297:2,4-Dinitrophenol
keywords: ROS; cell death; heat shock; hyperpolarization of inner mitochondrial membrane; mitochondria