title: Development of a chromatographic low pressure flow injection system using amperometric detection: Application to the analysis of niacin in coffee.
abstract: In this work, an analytical flow system able to perform low pressure chromatography with amperometric detection is presented. As case study, the determination of niacin (vitamin B3) in coffee brewed samples was selected. The manifold comprised a 1.0 cm length monolithic column coated with didecyldimethylammonium bromide, a laboratory-made boron doped diamond electrode, and featured in-line ionic strength adjustment of the mobile phase. The figures of merit concerning the selected case study namely, detection limit, 7.90 × 10(-7) M, determination rate, ca. 10 samplesh(-1), mobile phase and ISA solution consumption, ca. 2.6 mL per analysis, and CV, below 5% for retention time and peak height, showed the competitiveness of this analytical strategy comparing to the described HPLC methods for niacin determination. The strategy displays a simple configuration, low cost, fast and easy assembling, foreseeing its use to general purpose applications.
authors.affiliation: CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa/Porto, Rua Arquiteto Lobão Vital, Apartado 2511, 4202-401 Porto, Portugal. Electronic address: jrsantos@porto.ucp.pt.
authors.forename: João Rodrigo
authors.initials: JR
authors.lastname: Santos
authors.affiliation: CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa/Porto, Rua Arquiteto Lobão Vital, Apartado 2511, 4202-401 Porto, Portugal.
authors.forename: António O S S
authors.initials: AO
authors.lastname: Rangel
mesh_terms: D001895:Boron / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D040503:Coffea / Q000737:chemistry*; D003069:Coffee / Q000737:chemistry; D004566:Electrodes; D057230:Limit of Detection; D009525:Niacin / Q000032:analysis*; D010936:Plant Extracts / Q000032:analysis*; D012639:Seeds / Q000737:chemistry
chemical_list: D003069:Coffee; D010936:Plant Extracts; D009525:Niacin; D001895:Boron
keywords: Boron doped diamond electrode; Coffee; Flow analysis; Low pressure chromatography; Modified monolithic columns; Niacin; Vitamin B3