title: Thermal Stabilization of Proteins by Mono- and Oligosaccharides: Measurement and Analysis in the Context of an Excluded Volume Model.
abstract: The reversible thermal denaturation of apo α-lactalbumin and lysozyme was monitored via measurement of changes in absorbance and ellipticity in the presence of varying concentrations of seven mono- and oligosaccharides: glucose, galactose, fructose, sucrose, trehalose, raffinose, and stachyose. The temperature dependence of the unfolding curves was quantitatively accounted for by a two-state model, according to which the free energy of unfolding is increased by an amount that is independent of temperature and depends linearly upon the concentration of added saccharide. The increment of added unfolding free energy per mole of added saccharide was found to depend approximately linearly upon the extent of oligomerization of the saccharide. The relative strength of stabilization of different saccharide oligomers could be accounted for by a simplified statistical-thermodynamic model attributing the stabilization effect to volume exclusion deriving from steric repulsion between protein and saccharide molecules.
authors.affiliation: †Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, New Delhi 110025, India.
authors.forename: Ilyas
authors.initials: I
authors.lastname: Beg
authors.affiliation: ‡National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, United States.
authors.forename: Allen P
authors.initials: AP
authors.lastname: Minton
authors.affiliation: †Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, New Delhi 110025, India.
authors.forename: Imtaiyaz
authors.initials: I
authors.lastname: Hassan
authors.affiliation: †Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, New Delhi 110025, India.
authors.forename: Asimul
authors.initials: A
authors.lastname: Islam
authors.affiliation: †Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, New Delhi 110025, India.
authors.forename: Faizan
authors.initials: F
authors.lastname: Ahmad
mesh_terms: D000818:Animals; D001059:Apoproteins / Q000737:chemistry* / Q000378:metabolism; D002236:Carbohydrate Conformation; D002417:Cattle; D002645:Chickens; D004795:Enzyme Stability; D006358:Hot Temperature / Q000009:adverse effects; D007700:Kinetics; D007768:Lactalbumin / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D008970:Molecular Weight; D009005:Monosaccharides / Q000737:chemistry* / Q000378:metabolism; D009113:Muramidase / Q000737:chemistry* / Q000378:metabolism; D009844:Oligosaccharides / Q000737:chemistry* / Q000378:metabolism; D009994:Osmolar Concentration; D011487:Protein Conformation; D011489:Protein Denaturation; D055550:Protein Stability
chemical_list: D001059:Apoproteins; D009005:Monosaccharides; D009844:Oligosaccharides; C041966:apo-alpha-lactalbumin; D007768:Lactalbumin; C421864:hen egg lysozyme; D009113:Muramidase