title: Barcoding of fresh water fishes from Pakistan.
abstract: DNA bar-coding is a taxonomic method that uses small genetic markers in organisms' mitochondrial DNA (mt DNA) for identification of particular species. It uses sequence diversity in a 658-base pair fragment near the 5' end of the mitochondrial cytochrome c oxidase subunit 1 (CO1) gene as a tool for species identification. DNA barcoding is more accurate and reliable method as compared with the morphological identification. It is equally useful in juveniles as well as adult stages of fishes. The present study was conducted to identify three farm fish species of Pakistan (Cyprinus carpio, Cirrhinus mrigala, and Ctenopharyngodon idella) genetically. All of them belonged to family cyprinidae. CO1 gene was amplified. PCR products were sequenced and analyzed by bioinformatic software. Conspecific, congenric, and confamilial k2P nucleotide divergence was estimated. From these findings, it was concluded that the gene sequence, CO1, may serve as milestone for the identification of related species at molecular level.
authors.affiliation: a Department of Zoology , Government College of Science , Lahore , Pakistan and.
authors.forename: Asma
authors.initials: A
authors.lastname: Karim
authors.affiliation: a Department of Zoology , Government College of Science , Lahore , Pakistan and.
authors.forename: Asad
authors.initials: A
authors.lastname: Iqbal
authors.affiliation: a Department of Zoology , Government College of Science , Lahore , Pakistan and.
authors.forename: Rehan
authors.initials: R
authors.lastname: Akhtar
authors.affiliation: a Department of Zoology , Government College of Science , Lahore , Pakistan and.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Rizwan
authors.affiliation: b Department of Immunology , University of Health Sciences , Lahore , Pakistan.
authors.forename: Ali
authors.initials: A
authors.lastname: Amar
authors.affiliation: b Department of Immunology , University of Health Sciences , Lahore , Pakistan.
authors.forename: Usman
authors.initials: U
authors.lastname: Qamar
authors.affiliation: a Department of Zoology , Government College of Science , Lahore , Pakistan and.
authors.forename: Shah
authors.initials: S
authors.lastname: Jahan
mesh_terms: D000818:Animals; D019295:Computational Biology; D003530:Cyprinidae / Q000145:classification / Q000235:genetics*; D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D004272:DNA, Mitochondrial / Q000235:genetics; D005618:Fresh Water; D054629:Genome, Mitochondrial / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial
keywords: Cirrhinus mrigala; Ctenopharyngodon idella; Cyprinus carpio; DNA barcoding