title: The Rice High-Affinity Potassium Transporter1;1 Is Involved in Salt Tolerance and Regulated by an MYB-Type Transcription Factor.
abstract: Sodium transporters play key roles in plant tolerance to salt stress. Here, we report that a member of the High-Affinity K(+) Transporter (HKT) family, OsHKT1;1, in rice (Oryza sativa 'Nipponbare') plays an important role in reducing Na(+) accumulation in shoots to cope with salt stress. The oshkt1;1 mutant plants displayed hypersensitivity to salt stress. They contained less Na(+) in the phloem sap and accumulated more Na(+) in the shoots compared with the wild type. OsHKT1;1 was expressed mainly in the phloem of leaf blades and up-regulated in response to salt stress. Using a yeast one-hybrid approach, a novel MYB coiled-coil type transcription factor, OsMYBc, was found to bind to the OsHKT1;1 promoter. In vivo chromatin immunoprecipitation and in vitro electrophoresis mobility shift assays demonstrated that OsMYBc binds to AAANATNC(C/T) fragments within the OsHKT1;1 promoter. Mutation of the OsMYBc-binding nucleotides resulted in a decrease in promoter activity of OsHKT1;1. Knockout of OsMYBc resulted in a reduction in NaCl-induced expression of OsHKT1;1 and salt sensitivity. Taken together, these results suggest that OsHKT1;1 has a role in controlling Na(+) concentration and preventing sodium toxicity in leaf blades and is regulated by the OsMYBc transcription factor.
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
authors.forename: Wen
authors.initials: W
authors.lastname: Jing
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
authors.forename: Longyun
authors.identifier: http://orcid.org/0000-0002-9407-6895
authors.initials: L
authors.lastname: Xiao
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
authors.forename: Yakang
authors.initials: Y
authors.lastname: Jin
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
authors.forename: Like
authors.initials: L
authors.lastname: Shen
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, People's Republic of China whzhang@njau.edu.cn.
authors.forename: Wenhua
authors.identifier: http://orcid.org/0000-0003-3122-6152
authors.initials: W
authors.lastname: Zhang
mesh_terms: D060054:Agrobacterium / Q000502:physiology; D001483:Base Sequence; D027682:Cation Transport Proteins / Q000235:genetics / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D055786:Gene Knockout Techniques; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D012275:Oryza / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D018515:Plant Leaves / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018520:Plant Shoots / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D011188:Potassium / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D055049:Salt Tolerance / Q000187:drug effects / Q000235:genetics; D012964:Sodium / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological / Q000187:drug effects; D014026:Nicotiana / Q000382:microbiology; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D027682:Cation Transport Proteins; D010940:Plant Proteins; D014157:Transcription Factors; D012965:Sodium Chloride; D012964:Sodium; D011188:Potassium