title: Monoclonal antibody-based immunoassays for cyprodinil residue analysis in QuEChERS-based fruit extracts.
abstract: Cyprodinil is among the most common agrochemical residues found in highly perishable fruits, such as strawberries. In the present study, high-affinity monoclonal antibodies to this anilinopyrimidine fungicide were raised for the first time with the aim to produce valuable immunochemical analytical assays. Cyprodinil bioconjugates and the generated novel monoclonal antibodies were employed for sensitive competitive immunoassay development in two different formats. The limits of detection of the optimized assays were 20 and 30 ng L(-1) for the indirect and direct assay, respectively. Influence over assay parameters of different physicochemical factors was studied. Strawberry samples were extracted following the recommended QuEChERS procedure for pesticide residues in food, and analyzed by the optimized immunoassays. Recoveries and coefficients of variation from fortified samples were within standard values. In addition, the obtained immunochemical results with naturally contaminated samples were statistically comparable, according to Deming regression analysis, to those of a reference chromatographic method.
authors.affiliation: Department of Biotechnology, Institute of Agrochemistry and Food Technology, Consejo Superior de Investigaciones Científicas (IATA-CSIC), Agustí Escardino 7, 46980 Paterna, València, Spain.
authors.forename: Francesc A
authors.initials: FA
authors.lastname: Esteve-Turrillas
authors.affiliation: Department of Organic Chemistry, Universitat de València, Doctor Moliner 50, 46100 Burjassot, València, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Abad-Somovilla
authors.affiliation: Department of Organic Chemistry, Universitat de València, Doctor Moliner 50, 46100 Burjassot, València, Spain.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Quiñones-Reyes
authors.affiliation: Department of Organic Chemistry, Universitat de València, Doctor Moliner 50, 46100 Burjassot, València, Spain.
authors.forename: Consuelo
authors.initials: C
authors.lastname: Agulló
authors.affiliation: Department of Biotechnology, Institute of Agrochemistry and Food Technology, Consejo Superior de Investigaciones Científicas (IATA-CSIC), Agustí Escardino 7, 46980 Paterna, València, Spain. Electronic address: jvmercader@iata.csic.es.
authors.forename: Josep V
authors.initials: JV
authors.lastname: Mercader
authors.affiliation: Department of Biotechnology, Institute of Agrochemistry and Food Technology, Consejo Superior de Investigaciones Científicas (IATA-CSIC), Agustí Escardino 7, 46980 Paterna, València, Spain. Electronic address: aabad@iata.csic.es.
authors.forename: Antonio
authors.initials: A
authors.lastname: Abad-Fuentes
mesh_terms: D000911:Antibodies, Monoclonal / Q000032:analysis; D005506:Food Contamination / Q000032:analysis; D031985:Fragaria / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D005659:Fungicides, Industrial / Q000032:analysis* / Q000302:isolation & purification; D007118:Immunoassay / Q000379:methods*; D010573:Pesticide Residues / Q000032:analysis* / Q000302:isolation & purification; D011743:Pyrimidines / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D000911:Antibodies, Monoclonal; D005659:Fungicides, Industrial; D010573:Pesticide Residues; D011743:Pyrimidines; C108338:cyprodinil
keywords: Anilinopyrimidine; Cyprodinil (PubChem CID: 86367); ELISA; Fluodioxonil (PubChem CID: 86398); Food safety; Fungicide residues; Hapten; Mepanipyrim (PubChem CID: 86296); Picoxystrobin (PubChem CID: 11285653); Pyrimethanil (PubChem CID: 91650); Rapid methods