title: [Effects of biotin on blood glucose regulation in type 2 diabetes rat model].
abstract: OBJECTIVE
  To explore the effect of biotin on blood glucose regulation in rats and its possible mechanism.
  
  
  METHODS
  According to initial body weight and blood glucose, we randomly divided the 90 Wistar rats into 5 groups: the normal control group, model group, biotin low-dose group (0. 6mg/kg BW), biotin medium-dose group (3. 0 mg/kg BW) and biotin high-dose group (6. 0 mg/kg BW). After 2 months, the rats with HFS feed were injected with STZ (25 mg/kg BW) to manufacture diabetic rat model. After the OGTT experiment at 10th week, the blood glucose, insulin, liver/muscle glycogen and other biochemical indexes were detected. The GCK, PCK1 mRNA expression were measured with RT-PCR method.
  
  
  RESULTS
  Biotin has a certain improvement on postprandial glucose in diabetic rats. Compared with the model group, the AUC and the 30min postprandial blood glucose of biotin high-dose group were significantly decreased (P <0. 05). Biotin can affect some key enzyme gene in glucose metabolism, such as GCK, PCK1.
  
  
  CONCLUSIONS
  The possible mechanism of the decreasing biotin blood sugar in diabetic rats may by promoting the synthesis of glycogenand reducing gluconeogenesis.
authors.forename: Xuesong
authors.initials: X
authors.lastname: Xiang
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Xuesong
authors.initials: X
authors.lastname: Zhang
authors.forename: Wenzong
authors.initials: W
authors.lastname: Zhang
authors.forename: Zhu
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D001710:Biotin / Q000494:pharmacology*; D001786:Blood Glucose / Q000187:drug effects*; D001835:Body Weight; D050260:Carbohydrate Metabolism; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy; D005947:Glucose; D007328:Insulin; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001786:Blood Glucose; D007328:Insulin; D001710:Biotin; D005947:Glucose