title: HIV-1 Is Restricted prior to Integration of Viral DNA in Primary Cord-Derived Human CD34+ Cells.
abstract: Certain cells have the ability to block retroviral infection at specific stages of the viral cycle by the activities of well-characterized factors and transcriptional silencing machinery. Infection of murine stem cells (MSCs) by the murine leukemia viruses (MLVs) is profoundly blocked postintegration by transcriptional silencing. Here, we show that a dominant point of restriction of HIV-1 in human CD34+ cells is prior to integration of viral DNA and that HIV-1 restriction by human CD34+ cells is fundamentally different from MLV restriction by mouse cells.
authors.forename: Daniel O
authors.initials: DO
authors.lastname: Griffin
authors.forename: Stephen P
authors.initials: SP
authors.lastname: Goff
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000276:immunology; D004279:DNA, Viral / Q000235:genetics* / Q000378:metabolism; D015658:HIV Infections / Q000276:immunology / Q000821:virology*; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000276:immunology* / Q000821:virology; D006801:Humans; D009052:Leukemia Virus, Murine / Q000235:genetics / Q000502:physiology; D051379:Mice; D014470:Umbilical Cord / Q000166:cytology* / Q000276:immunology / Q000821:virology; D016662:Virus Integration; D014779:Virus Replication
chemical_list: D018952:Antigens, CD34; D004279:DNA, Viral