title: An atypical Toxoplasma gondii genotype in a rural Brazilian dog co-infected with Leishmania (Viannia) braziliensis.
abstract: Toxoplasmosis and leishmaniasis are two worldwide zoonoses caused by the protozoan parasites Toxoplasma gondii and Leishmania spp., respectively. This report describes the clinical and laboratorial findings of a co-infection with both parasites in a 4-year-old female dog suspected of ehrlichiosis that presented anemia, thrombocytopenia, hypoalbuminemia, hyperglobulinemia, tachyzoite-like structures to the lung imprints, and polymerase chain reaction (PCR) results positive for T. gondii (kidney, lung, and liver) and Leishmania spp. Co-infection with Toxoplasma gondii and Leishmania braziliensis was confirmed by sequencing; restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) confirmed an atypical T. gondii genotype circulating in dogs that has been reported to cause human congenital toxoplasmosis.
authors.affiliation: Department of Pathobiology and Population Medicine, College of Veterinary Medicine, Mississippi State University, Mississippi, USA.
authors.forename: Rodrigo Costa da
authors.initials: RC
authors.lastname: Silva
authors.affiliation: Departamento de Higiene Veterinária e Saúde Pública, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Botucatu, São Paulo, Brazil.
authors.forename: Karen
authors.initials: K
authors.lastname: Caffaro
authors.affiliation: Departamento de Higiene Veterinária e Saúde Pública, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Botucatu, São Paulo, Brazil.
authors.forename: Carolina Lechinski
authors.initials: CL
authors.lastname: Paula
authors.affiliation: Departamento de Higiene Veterinária e Saúde Pública, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Botucatu, São Paulo, Brazil.
authors.forename: Rafaela Mastrangelo
authors.initials: RM
authors.lastname: Risseti
authors.affiliation: Departamento de Higiene Veterinária e Saúde Pública, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Botucatu, São Paulo, Brazil.
authors.forename: Helio
authors.initials: H
authors.lastname: Langoni
authors.affiliation: Departamento de Higiene Veterinária e Saúde Pública, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Botucatu, São Paulo, Brazil.
authors.forename: Jane
authors.initials: J
authors.lastname: Megid
authors.affiliation: Departamento de Clínica Veterinária, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Botucatu, São Paulo, Brazil.
authors.forename: Mariana Serrano
authors.initials: MS
authors.lastname: Melanchauski
authors.affiliation: Departamento de Clínica Veterinária, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Botucatu, São Paulo, Brazil.
authors.forename: Katiane Lohn
authors.initials: KL
authors.lastname: Souza
authors.affiliation: Departamento de Clínica Veterinária, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Botucatu, São Paulo, Brazil.
authors.forename: Regina Kiomi
authors.initials: RK
authors.lastname: Takahira
authors.affiliation: Departamento de Reprodução Animal e Radiologia Veterinária, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Botucatu, São Paulo, Brazil.
authors.forename: Vânia Maria de Vasconcelos
authors.initials: VM
authors.lastname: Machado
mesh_terms: D000818:Animals; D060085:Coinfection / Q000469:parasitology / Q000662:veterinary; D016054:DNA, Protozoan; D004283:Dog Diseases / Q000175:diagnosis / Q000469:parasitology*; D004285:Dogs; D005260:Female; D005838:Genotype; D007892:Leishmania braziliensis / Q000235:genetics*; D007896:Leishmaniasis / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D014122:Toxoplasma / Q000235:genetics*; D014124:Toxoplasmosis, Animal / Q000175:diagnosis / Q000469:parasitology*
chemical_list: D016054:DNA, Protozoan