title: Deciphering the Positional Influence of the Hydroxyl Group in the Cinnamoyl Part of 3-Hydroxy Flavonoids for Structural Modification and Their Interaction with the Protonated and B Form of Calf Thymus DNA Using Spectroscopic and Molecular Modeling Studies.
abstract: Studies on the interaction of naturally occurring flavonoids with different polymorphic forms of nucleic acid are helpful for understanding the molecular aspects of binding mode and providing direction for the use and design of new efficient therapeutic agents. However, much less information is available on the interactions of these compounds with different polymorphic forms of DNA at the molecular level. In this report we investigated the interaction of two widely abundant dietary flavonoids quercetin (Q) and morin (M) with calf thymus (CT) DNA. Spectrophotometric, spectropolarimetric, viscosity measurement, and molecular docking simulation methods are used as tools to delineate the binding mode and probable location of the flavonoids and their effects on the stability and conformation of DNA. It is observed that in the presence of the protonated form of DNA the dual fluorescence of Q and M resulting from the excited-state intramolecular proton transfer (ESIPT) is modified significantly. Structural analysis showed Q and M binds weakly to the B form (groove binding) compared to the protonated form of CT DNA (electrostatic interaction). In both cases, Q binds strongly to both forms of DNA compared to M.
authors.affiliation: †Department of Chemistry, Jadavpur University, Raja S. C. Mullick Road, Jadavpur, Kolkata, West Bengal 700032, India.
authors.forename: Ankur Bikash
authors.initials: AB
authors.lastname: Pradhan
authors.affiliation: †Department of Chemistry, Jadavpur University, Raja S. C. Mullick Road, Jadavpur, Kolkata, West Bengal 700032, India.
authors.forename: Lucy
authors.initials: L
authors.lastname: Haque
authors.affiliation: †Department of Chemistry, Jadavpur University, Raja S. C. Mullick Road, Jadavpur, Kolkata, West Bengal 700032, India.
authors.forename: Sutanwi
authors.initials: S
authors.lastname: Bhuiya
authors.affiliation: ‡University of Calcutta, Senate House, 87/1, College Street, Kolkata, West Bengal 700073, India.
authors.forename: Aniruddha
authors.initials: A
authors.lastname: Ganguly
authors.affiliation: †Department of Chemistry, Jadavpur University, Raja S. C. Mullick Road, Jadavpur, Kolkata, West Bengal 700032, India.
authors.forename: Suman
authors.initials: S
authors.lastname: Das
mesh_terms: D000818:Animals; D016880:Anisotropy; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D059371:DNA, B-Form / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D017665:Hydroxyl Radical / Q000737:chemistry; D008956:Models, Chemical; D008957:Models, Genetic; D062105:Molecular Docking Simulation; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D011522:Protons; D011794:Quercetin / Q000737:chemistry*; D013057:Spectrum Analysis; D014783:Viscosity; D047309:Flavones
chemical_list: D004247:DNA; D059371:DNA, B-Form; D005419:Flavonoids; D017665:Hydroxyl Radical; D011522:Protons; D011794:Quercetin; C008548:morin; D047309:Flavones; C427203:calf thymus DNA