title: Recent advances and current issues in single-cell sequencing of tumors.
abstract: Intratumoral heterogeneity is a recently recognized but important feature of cancer that underlies the various biocharacteristics of cancer tissues. The advent of next-generation sequencing technologies has facilitated large scale capture of genomic data, while the recent development of single-cell sequencing has allowed for more in-depth studies into the complex molecular mechanisms of intratumoral heterogeneity. In this review, the recent advances and current challenges in single-cell sequencing methodologies are discussed, highlighting the potential power of these data to provide insights into oncological processes, from tumorigenesis through progression to metastasis and therapy resistance.
authors.affiliation: Institute of Immunology, Third Military Medical University, Chongqing 400038, China.
authors.forename: Hai-Jian
authors.initials: HJ
authors.lastname: Sun
authors.affiliation: Institute of Immunology, Third Military Medical University, Chongqing 400038, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.affiliation: Institute of Immunology, Third Military Medical University, Chongqing 400038, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Ni
authors.affiliation: Institute of Immunology, Third Military Medical University, Chongqing 400038, China. Electronic address: oceanyx@126.com.
authors.forename: Xia
authors.initials: X
authors.lastname: Yang
authors.affiliation: Institute of Immunology, Third Military Medical University, Chongqing 400038, China. Electronic address: wuyuzhang@tmmu.edu.cn.
authors.forename: Yu-Zhang
authors.initials: YZ
authors.lastname: Wu
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D002469:Cell Separation; D019008:Drug Resistance, Neoplasm / Q000235:genetics; D020022:Genetic Predisposition to Disease; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D058990:Molecular Targeted Therapy; D009369:Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D010641:Phenotype; D057285:Precision Medicine; D011237:Predictive Value of Tests; D017422:Sequence Analysis, DNA / Q000379:methods*; D059010:Single-Cell Analysis / Q000379:methods*; D059467:Transcriptome
chemical_list: D000970:Antineoplastic Agents; D014408:Biomarkers, Tumor
keywords: Intratumoral heterogeneity; Sequencing analysis; Single-cell sequencing; Tumor