title: Two new triterpenoid saponins from Ilex cornuta.
abstract: Two new triterpenoid saponins (1 and 2) were isolated from the stems of Ilex cornuta, along with two known triterpenoids (3 and 4). The structures of compounds 1 and 2 were determined as ursane-12,19-diene-28-oic acid 3β-O-β-D-glucuronopyranoside-6-O-methyl ester (1), 3α,23α-dihydroxy-olean-9(11),12-diene-28-oic acid 28-O-β-D-glucopyranosyl ester (2), on the basis of hydrolysis and spectral evidence, including 1D and 2D NMR and high resolution electrospray ionization mass spectrometry (HR-ESI-MS) analyses. Protective effects of compounds 1-4 were tested against H(2)O(2)-induced H9c2 cardiomyocyte injury, and the data showed that all these compounds had no cell-protective effect.
authors.affiliation: a College of Pharmaceutical Science, Soochow University , Suzhou 215123 , China.
authors.forename: Ming-Lei
authors.initials: ML
authors.lastname: Wang
authors.affiliation: a College of Pharmaceutical Science, Soochow University , Suzhou 215123 , China.
authors.forename: Yan-Li
authors.initials: YL
authors.lastname: Liu
authors.affiliation: a College of Pharmaceutical Science, Soochow University , Suzhou 215123 , China.
authors.forename: Qiong-Ming
authors.initials: QM
authors.lastname: Xu
authors.affiliation: b College of Pharmacy, Guilin Medical University , Guilin 541004 , China.
authors.forename: Yun-Qiu
authors.initials: YQ
authors.lastname: Li
authors.affiliation: a College of Pharmaceutical Science, Soochow University , Suzhou 215123 , China.|b College of Pharmacy, Guilin Medical University , Guilin 541004 , China.
authors.forename: Shi-Lin
authors.initials: SL
authors.lastname: Yang
mesh_terms: D006861:Hydrogen Peroxide / Q000494:pharmacology; D030017:Ilex / Q000737:chemistry*; D015394:Molecular Structure; D032383:Myocytes, Cardiac / Q000187:drug effects; D019906:Nuclear Magnetic Resonance, Biomolecular; D018517:Plant Roots / Q000737:chemistry; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification*; D013237:Stereoisomerism; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification*; D053978:Pentacyclic Triterpenes
chemical_list: D006861:Hydrogen Peroxide; D012503:Saponins; D014315:Triterpenes; C000603712:3alpha,23alpha-dihydroxy-olean-9(11),12-diene-28-oic acid 28-O-beta-D-glucopyranosyl ester; C000603711:ursane-12,19-diene-28-oic acid 3beta-O-beta-D-glucuronopyranoside-6-O-methyl ester; D053978:Pentacyclic Triterpenes
keywords: 12-diene-28-oic acid 28-O-β-d-glucopyranosyl ester; 19-diene-28-oic acid 3β-O-β-d-glucuronopyranoside-6-O-methyl ester; 23α-dihydroxy-olean-9(11); 3α; Aquifoliaceae; Ilex cornuta; cardiomyocyte oxidative injury; cell-protective effect; ursane-12