title: Anti-inflammatory effects of isoacteoside from Abeliophyllum distichum.
abstract: Isoacteoside, a dihydroxypheynylethyl glycoside, is a major bioactive component of Abeliophyllum distichum (White Forsythia) which is a deciduous shrub native to the south and central areas of Korea. The present study is designed to evaluate the anti-inflammatory activities and underlying mechanisms of isoacteoside in human mast cell line, HMC-1 cells. We isolated isoacteoside from A. distichum. The anti-inflammatory effect of isoacteoside was investigated in HMC-1 cells by studying the following markers: phorbol 12-myristate 13-acetate and calcium ionophore A23187 (PMACI)-induced interleukin (IL)-1β, IL-6, IL-8, and tumor necrosis factor alpha (TNF-α) secretion and mRNA expression by ELISA and RT-PCR, respectively. In addition, mechanism related to anti-inflammatory was investigated by Western blotting. Isoacteoside significantly suppressed the production and mRNA expression of proinflammatory cytokines including IL-1β, IL-6, IL-8 and TNF-α in PMACI-stimulated HMC-1 cells without cytotoxicity. It was found that anti-inflammatory effects of isoacteoside are mediated by action on caspase-1, mitogen-activated protein kinases (c-Jun N-terminal kinase, p38, extracellular signal-regulated protein kinase) and nuclear factor-kappa B pathways. Taken together, the present findings provide new insights that isoacteoside may be a promising anti-inflammatory agent for inflammatory disorders.
authors.affiliation: Department of Pharmacology, College of Korean Medicine, Kyung Hee University , Seoul , Republic of Korea .
authors.forename: Sun-Young
authors.initials: SY
authors.lastname: Nam
authors.forename: Hee-Yun
authors.initials: HY
authors.lastname: Kim
authors.forename: Myoung-Schook
authors.initials: MS
authors.lastname: Yoou
authors.forename: A Hyun
authors.initials: AH
authors.lastname: Kim
authors.forename: Byoung Jun
authors.initials: BJ
authors.lastname: Park
authors.forename: Hyun-Ja
authors.initials: HJ
authors.lastname: Jeong
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Kim
mesh_terms: D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002460:Cell Line; D016207:Cytokines / Q000276:immunology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000276:immunology; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000178:diet therapy / Q000276:immunology / Q000473:pathology; D020935:MAP Kinase Signaling System / Q000187:drug effects* / Q000276:immunology; D008407:Mast Cells / Q000276:immunology* / Q000473:pathology; D029592:Oleaceae / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D005960:Glucosides; D010636:Phenols; C064683:isoacteoside; D048049:Extracellular Signal-Regulated MAP Kinases
keywords: Caspase-1; MAPKs; NF-κB; isoacteoside; mast cells