title: Probiotic Mixture KF Attenuates Age-Dependent Memory Deficit and Lipidemia in Fischer 344 Rats.
abstract: To investigate the memory-enhancing effect of lactic acid bacteria, we selected the probiotic mixture KF, which consisted of Lactobacillus plantarum KY1032 and Lactobacillus curvatus HY7601 (1 × 10(11) CFU/g of each strain), and investigated its antilipidemic and memoryenhancing effects in aged Fischer 344 rats. KF (1 × 10(10) CFU/rat/day), which was administered orally once a day (6 days per week) for 8 weeks, significantly inhibited age-dependent increases of blood triglyceride and reductions of HDL cholesterol (p < 0.05). KF restored agereduced spontaneous alternation in the Y-maze task to 94.4% of that seen in young rats (p < 0.05). KF treatment slightly, but not significantly, shortened the escape latency daily for 4 days. Oral administration of KF restored age-suppressed doublecortin and brain-derived neurotrophic factor expression in aged rats. Orally administered KF suppressed the expression of p16, p53, and cyclooxygenase-2, the phosphorylation of Akt and mTOR, and the activation of NF-κB in the hippocampus of the brain. These findings suggest that KF may ameliorate age-dependent memory deficit and lipidemia by inhibiting NF-κB activation.
authors.affiliation: Department of Life and Nanopharmaceutical Sciences, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Jin-Ju
authors.initials: JJ
authors.lastname: Jeong
authors.affiliation: Department of Life and Nanopharmaceutical Sciences, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Kyung-Ah
authors.initials: KA
authors.lastname: Kim
authors.affiliation: R&BD Center, Korea Yakult Co., Ltd., Yongin 449-901, Republic of Korea.
authors.forename: Young-Tae
authors.initials: YT
authors.lastname: Ahn
authors.affiliation: R&BD Center, Korea Yakult Co., Ltd., Yongin 449-901, Republic of Korea.
authors.forename: Jae-Hun
authors.initials: JH
authors.lastname: Sim
authors.affiliation: Department of Life and Nanopharmaceutical Sciences, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Jae-Yeon
authors.initials: JY
authors.lastname: Woo
authors.affiliation: Graduate School of International Agricultural Technology/GBST, Seoul National University, Gangwon 232-916, Republic of Korea.
authors.forename: Chul-Sung
authors.initials: CS
authors.lastname: Huh
authors.affiliation: Department of Life and Nanopharmaceutical Sciences, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Dong-Hyun
authors.initials: DH
authors.lastname: Kim
mesh_terms: D000284:Administration, Oral; D000818:Animals; D008076:Cholesterol, HDL / Q000097:blood; D000089662:Doublecortin Protein; D006624:Hippocampus / Q000187:drug effects / Q000473:pathology; D006949:Hyperlipidemias / Q000517:prevention & control*; D007778:Lactobacillus; D008569:Memory Disorders / Q000517:prevention & control*; D019936:Probiotics / Q000008:administration & dosage*; D011916:Rats, Inbred F344; D014280:Triglycerides / Q000097:blood
chemical_list: D008076:Cholesterol, HDL; C000714848:Dcx protein, rat; D000089662:Doublecortin Protein; D014280:Triglycerides
keywords: Lactobacillus curvatus; Lactobacillus plantarum; aging; lipidemia; memory deficit