title: In vivo study on splenomegaly inhibition by genistein in Plasmodium berghei-infected mice.
abstract: Spleen plays an important role in removing old and damaged red blood cells and malaria-infected erythrocytes. When malaria parasites invade the spleen and induce splenomegaly, splenic function tends to be impaired. Thus, the inhibition of splenomegaly is strongly required to protect the spleen. In this study, malaria-induced splenomegaly is inhibited by injecting genistein into a Plasmodium berghei-infected ICR mouse. To explain this phenomenon, the effect of genistein in spleen and liver of malaria-infected mice was evaluated by histological examination. Malaria parasites disrupted splenic architecture. After treating genistein, the disrupted architecture in which red and white pulp regions were clearly separated in recovered to uninfected ones. Changes in biophysical properties of blood were studied by measuring the viscosity of blood collected from malaria-infected and uninfected mice using a microfluidic viscometer. Genistein also had a negligible influence on variation in blood viscosity. The enzymatic activity and expression pattern of proteins were then investigated to explain the genistein effect on malaria-induced splenomegaly. Genistein is a potential drug for splenomegaly in P. berghei-infected mouse.
authors.affiliation: Division of Integrative Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea.
authors.forename: Young Ran
authors.initials: YR
authors.lastname: Ha
authors.affiliation: Department of Mechanical Engineering, Chosun University, Gwangju 501-759, Republic of Korea.
authors.forename: Yang-Jun
authors.initials: YJ
authors.lastname: Kang
authors.affiliation: Center for Biofluid and Biomimic Research, Department of Mechanical Engineering, Pohang University of Science and Technology, San 31, Hyojadong, Namgu, Pohang, Kyungbook 790-784, Republic of Korea. Electronic address: sjlee@postech.ac.kr.
authors.forename: Sang Joon
authors.initials: SJ
authors.lastname: Lee
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D004912:Erythrocytes; D005786:Gene Expression Regulation / Q000187:drug effects; D019833:Genistein / Q000008:administration & dosage / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008288:Malaria / Q000188:drug therapy / Q000469:parasitology* / Q000473:pathology; D008297:Male; D020782:Matrix Metalloproteinases / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D010962:Plasmodium berghei; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D013154:Spleen / Q000473:pathology; D013163:Splenomegaly / Q000469:parasitology* / Q000473:pathology / Q000517:prevention & control
chemical_list: D047428:Protein Kinase Inhibitors; D019833:Genistein; D020782:Matrix Metalloproteinases
keywords: Blood viscosity; Erythrocyte; Genistein; Plasmodium berghei; Spleen; Splenomegaly