title: Uygur medicine Xipayi Kui Jie'an affects gene expression profiles in intestinal tissue lesions in a rat model of ulcerative colitis.
abstract: BACKGROUND
  The aim of this study was to investigate the mechanisms underlying the therapeutic effect of Uygur medicine KJA on UC in a rat model.
  
  
  METHODS
  UC was induced in Wistar rats by application of 2, 4-dinitrochlorobenzene and acetic acid and were then treated with three different doses of KJA, and normal saline as control. After treatment for 20 days, the gene expression profile of colonic tissue was analyzed by microarray and verified by quantitative real-time RT-PCR.
  
  
  RESULTS
  Animals treated with the three different doses of KJA were compared with normal saline controls, wherein microarray analysis identified 1991, 2163, and 1677 differentially expressed genes respectively, of which 444 genes were raised and 670 genes were decrease spliced together in the three doses tested. The KEGG pathway analyses found commonly raised genes related to several different biological functions. Interesting genes included TRL2, IL-1β, TGF-β1, and NF-κB were confirmed by quantitative PCR.
  
  
  CONCLUSIONS
  The therapeutic effect of KJA on UC is likely explained by specific effects on the expression of genes, which are the effector molecules known to be involved in the development of UC. Further studies on differentially expressed genes will help explain the mechanism of action of Uygur medicine KJA.
authors.affiliation: Department of Molecular Biology and Biochemistry, College of Basic Medicine, Xinjiang Medical University, Urumqi, Xinjiang, 830000, China. kurax8824@sina.com.
authors.forename: Kurexi
authors.initials: K
authors.lastname: Yunusi
authors.affiliation: Department of Molecular Biology and Biochemistry, College of Basic Medicine, Xinjiang Medical University, Urumqi, Xinjiang, 830000, China. 9601874@qq.com.
authors.forename: Jingping
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Molecular Biology and Biochemistry, College of Basic Medicine, Xinjiang Medical University, Urumqi, Xinjiang, 830000, China. 397597052@qq.com.
authors.forename: Li
authors.initials: L
authors.lastname: Zhong
authors.affiliation: Department of Molecular Biology and Biochemistry, College of Basic Medicine, Xinjiang Medical University, Urumqi, Xinjiang, 830000, China. 773658703@qq.com.
authors.forename: Gulinisha
authors.initials: G
authors.lastname: Mosha
authors.affiliation: Department of Molecular Biology and Biochemistry, College of Basic Medicine, Xinjiang Medical University, Urumqi, Xinjiang, 830000, China. 591893513@qq.com.
authors.forename: Ajiguli
authors.initials: A
authors.lastname: Nuermaimaiti
authors.affiliation: Department of Molecular Biology and Biochemistry, College of Basic Medicine, Xinjiang Medical University, Urumqi, Xinjiang, 830000, China. 1458336635@qq.com.
authors.forename: Mairipaiti
authors.initials: M
authors.lastname: Abudula
authors.affiliation: Traditional Uighur Medicine Institute, Xinjiang Medical University, Urumqi, Xinjiang, 830000, China. zjp_1215@163.com.
authors.forename: Halmurat
authors.initials: H
authors.lastname: Upur
mesh_terms: D000818:Animals; D003093:Colitis, Ulcerative / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D005707:Gallic Acid / Q000494:pharmacology* / Q000627:therapeutic use; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects* / Q000473:pathology; D008297:Male; D008519:Medicine, Traditional; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D059467:Transcriptome / Q000187:drug effects*; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism
chemical_list: D016207:Cytokines; D053583:Interleukin-1beta; D016328:NF-kappa B; D010936:Plant Extracts; D053773:Transforming Growth Factor beta1; D005707:Gallic Acid