title: Study on the anaphylactoid of three phenolic acids in Honeysuckle.
abstract: BACKGROUND
  Chlorogenic acid (CA) is one of the major ingredients in Honeysuckle which exhibits anticancer, antibacterial, antiviral, hypoglycemic and anti-HIV activities. However, with the frequent emergence of anaphylactoid reactions of traditional Chinese medicine (TCM) injections which contains Honeysuckle in recent years, many researchers found that CA exhibited allergenicity.
  
  
  OBJECTIVE
  To explore the changes of content of CA, neochlorogenic acid (NCA) and cryptochlorogenic acid (CCA) during the preparation of Honeysuckle and evaluate the anaphylactoid of these three ingredients.
  
  
  METHODS
  Two extracts of Honeysuckle were prepared by different methods and the content of CA, NCA and CCA were determined by high performance liquid chromatography (HPLC) analysis. Guinea pig and mast cells RBL-2H3 were utilized as the animal and cell model to investigate the anaphylactoid of these three ingredients.
  
  
  RESULTS
  The content of CA decreased while CCA and NCA increased during the preparation of Honeysuckle. In vitro and in vivo studies showed that CA and CCA could significantly increase the plasma serotonin and β-hexosaminidase levels in guinea pigs and induce the degranulation of RBL-2H3 cell, while NCA did not show such properties. In addition, CAA had a stronger effect than CA.
  
  
  CONCLUSIONS
  We believed that both CA and CCA could cause anaphylactoid reaction while NCA could not. Moreover, the anaphylactoid of CCA is higher than CA. Our result demonstrated that CA is not the only cause of anaphylactoid reactions of TCM injections which contains Honeysuckle.
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR China; State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Jiangsu, PR China.
authors.forename: Cunyu
authors.initials: C
authors.lastname: Li
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR China; State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Jiangsu, PR China.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR China; State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Jiangsu, PR China. Electronic address: guopingpeng@sohu.com.
authors.forename: Guoping
authors.initials: G
authors.lastname: Peng
mesh_terms: D000707:Anaphylaxis / Q000209:etiology* / Q000276:immunology; D000818:Animals; D002460:Cell Line; D002726:Chlorogenic Acid / Q000009:adverse effects* / Q000276:immunology / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D006168:Guinea Pigs; D062385:Hydroxybenzoates / Q000009:adverse effects* / Q000276:immunology / Q000302:isolation & purification; D031239:Lonicera / Q000737:chemistry*; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology; D008516:Medicine, Chinese Traditional / Q000009:adverse effects; D051381:Rats
chemical_list: D062385:Hydroxybenzoates; D002726:Chlorogenic Acid; C017616:phenolic acid
keywords: Anaphylactoid reaction; Chlorogenic acid; Chlorogenic acid (PubChem CID: 1794427); Cryptochlorogenic acid; Cryptochlorogenic acid (PubChem CID: 9798666); Honeysuckle; Methyl thiazolyl tetrazolium (PubChem CID: 16218671); Neochlorogenic acid; Neochlorogenic acid (PubChem CID: 5280633)