title: Detection of cytomegalovirus, human parvovirus B19, and herpes simplex virus-1/2 in women with first-trimester spontaneous abortions.
abstract: The relationship between viral infections and first-trimester spontaneous abortions is not well-understood. The study aim was to investigate the prevalence of cytomegalovirus (CMV), human parvovirus B19 (B19V), and herpes simplex virus-1/2 (HSV-1/2) infection by molecular and serological techniques in women experiencing spontaneous miscarriage in the first trimester of pregnancy. Plasma samples were examined for CMV, B19V, and HSV-1/2 DNA using real-time quantitative polymerase chain reaction (Real-time qPCR), and for specific IgG antibodies against B19V, CMV, and HSV-1/2 using serological assays. The abortion group consisted of women (n = 1,716) with a history of two or more first-trimester spontaneous abortions. Women younger than 30 years possess higher portion to experience spontaneous abortion. No specimens were positive for B19V or CMV DNA. Seven out of the 1,716 specimens were positive for HSV-1/2 DNA. By serology, 47.24% of patients were positive for B19V IgG, 39.66% for HSV IgG, 79.31% for CMV IgG, and 9.31% for B19V IgM. The high rate of positivity for CMV IgG suggests that the majority of women with first-trimester spontaneous abortions are not susceptible to primary CMV infection. The lack of virus DNA in the majority of cases indicates that B19V, CMV, and HSV-1/2 infection is not commonly associated with first-trimester spontaneous abortion.
authors.affiliation: Institute of Blood Transfusion, Chinese Academy of Medical Sciences, Chengdu, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Institute of Blood Transfusion, Chinese Academy of Medical Sciences, Chengdu, China.
authors.forename: Guohui
authors.initials: G
authors.lastname: Bian
authors.affiliation: Institute of Blood Transfusion, Chinese Academy of Medical Sciences, Chengdu, China.
authors.forename: Qiongxiu
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Institute of Blood Transfusion, Chinese Academy of Medical Sciences, Chengdu, China.
authors.forename: Zhan
authors.initials: Z
authors.lastname: Gao
authors.affiliation: The Third People's Hospital of Chongqing, Chongqing, China.
authors.forename: Pu
authors.initials: P
authors.lastname: Liao
authors.affiliation: Institute of Blood Transfusion, Chinese Academy of Medical Sciences, Chengdu, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Blood Transfusion, Chinese Academy of Medical Sciences, Chengdu, China.
authors.forename: Miao
authors.initials: M
authors.lastname: He
mesh_terms: D000022:Abortion, Spontaneous / Q000821:virology*; D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000914:Antibodies, Viral / Q000097:blood; D003587:Cytomegalovirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification* / Q000472:pathogenicity; D003586:Cytomegalovirus Infections / Q000175:diagnosis; D004279:DNA, Viral / Q000097:blood*; D005260:Female; D006561:Herpes Simplex / Q000175:diagnosis; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D018258:Herpesvirus 2, Human / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008875:Middle Aged; D010322:Parvoviridae Infections / Q000175:diagnosis; D016732:Parvovirus B19, Human / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000821:virology*; D011261:Pregnancy Trimester, First; D060888:Real-Time Polymerase Chain Reaction; D055815:Young Adult
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D007074:Immunoglobulin G
keywords: Herpes simplex virus-1/2; Human parvovirus B19; Real-time qPCR; cytomegalovirus; first-trimester spontaneous abortion