title: Inhibition of HMG-CoA reductase by MFS, a purified extract from the fermentation of marine fungus Fusarium solani FG319, and optimization of MFS production using response surface methodology.
abstract: The present study was designed to isolate and characterize a purified extract from Fusarium solani FG319, termed MFS (Metabolite of Fusarium solani FG319) that showed anti-atherosclerosis activity by inhibiting 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase. Response surface methodology (RSM) was employed to achieve an improved yield from the fermentation medium. The inhibiting effect of the isolate, MFS, on HMG-CoA reductase was greater than that of the positive control, lovastatin. The average recovery of MFS and the relative standard deviation (RSD) ranged between 99.75% to 101.18%, and 0.31% to 0.74%, respectively. The RSDs intra- and inter-assay of the three samples ranged from 0.288% to 2.438%, and from 0.934% to 2.383%, respectively. From the RSM, the concentration of inducer, cultivation time, and culture temperatures had significant effects on the MFS production, with the effect of inducer concentration being more pronounced that other factors. In conclusion, the optimal conditions for the MFS production were achieved using RSM and that MFS could be explored as an anti-atherosclerosis agent based on its ability to inhibit HMG-CoA reductase.
authors.affiliation: College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; Shanghai Aquatic Products Processing and Storage Engineering Technology Research Center, Shanghai 201306, China; Institute of Marine Science, Shanghai Ocean University, Shanghai 201306, China.
authors.forename: Wen-Hui
authors.initials: WH
authors.lastname: Wu
authors.affiliation: College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
authors.forename: Qing-Bo
authors.initials: QB
authors.lastname: Zhao
authors.affiliation: College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Wang
authors.affiliation: College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; Shanghai Aquatic Products Processing and Storage Engineering Technology Research Center, Shanghai 201306, China. Electronic address: bbao@shou.edu.cn.
authors.forename: Bin
authors.initials: B
authors.lastname: Bao
mesh_terms: D000704:Analysis of Variance; D001685:Biological Factors / Q000302:isolation & purification / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation / Q000502:physiology; D005670:Fusarium / Q000378:metabolism*; D006903:Hydroxymethylglutaryl CoA Reductases / Q000378:metabolism*; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D008148:Lovastatin / Q000494:pharmacology; D021141:Nucleic Acid Amplification Techniques
chemical_list: D001685:Biological Factors; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D008148:Lovastatin; D006903:Hydroxymethylglutaryl CoA Reductases
keywords: HMG-CoA reductase; HPLC analysis; Metabolite; Response surface methodology