title: Proteomic analysis of the excretory/secretory products and antigenic proteins of Echinococcus granulosus adult worms from infected dogs.
abstract: BACKGROUND
  Cystic echinococcosis, which is caused by Echinococcus granulosus, is one of the most widespread zoonotic helminth diseases that affects humans and livestock. Dogs, which harbor adult worms in their small intestines, are a pivotal source of E. granulosus infection in humans and domestic animals. Therefore, novel molecular approaches for the prevention and diagnosis of this parasite infection in dogs need to be developed.
  
  
  RESULTS
  In this study, we performed proteomic analysis to identify excretory/secretory products (ES) and antigenic proteins of E. granulosus adult worms using two-dimensional electrophoresis, tandem matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF/TOF), and Western blotting of sera from infected dogs. This study identified 33 ES product spots corresponding to 9 different proteins and 21 antigenic protein spots corresponding to 13 different proteins. Six antigenic proteins were identified for the first time.
  
  
  CONCLUSIONS
  The present study extended the existing proteomic data of E. granulosus and provides further information regarding host-parasite interactions and survival mechanisms. The results of this study contribute to vaccination and immunodiagnoses for E. granulosus infections.
authors.affiliation: National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention; Laboratory of Parasite and Vector Biology, MOH, China; WHO Collaborating Center for Malaria, Schistosomiasis and Filariasis, Shanghai, 200025, China. wangying1162@163.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China. xiaodi@icdc.cn.
authors.forename: Di
authors.initials: D
authors.lastname: Xiao
authors.affiliation: National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention; Laboratory of Parasite and Vector Biology, MOH, China; WHO Collaborating Center for Malaria, Schistosomiasis and Filariasis, Shanghai, 200025, China. amyshyj12@163.com.
authors.forename: Yujuan
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Qinghai Institute for Endemic Disease Prevention and Control, Xining, 811602, China. qhxn_66@163.com.
authors.forename: Xiuming
authors.initials: X
authors.lastname: Han
authors.affiliation: Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China. zhaofei@icdc.cn.
authors.forename: Fei
authors.initials: F
authors.lastname: Zhao
authors.affiliation: National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention; Laboratory of Parasite and Vector Biology, MOH, China; WHO Collaborating Center for Malaria, Schistosomiasis and Filariasis, Shanghai, 200025, China. li.xiaohong@york.ac.uk.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Li
authors.affiliation: National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention; Laboratory of Parasite and Vector Biology, MOH, China; WHO Collaborating Center for Malaria, Schistosomiasis and Filariasis, Shanghai, 200025, China. wuweiping@hotmail.com.
authors.forename: Weiping
authors.initials: W
authors.lastname: Wu
authors.affiliation: National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention; Laboratory of Parasite and Vector Biology, MOH, China; WHO Collaborating Center for Malaria, Schistosomiasis and Filariasis, Shanghai, 200025, China. zhouhejun2@126.com.
authors.forename: Hejun
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China. zhangjianzhong@icdc.com.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Zhang
authors.affiliation: National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention; Laboratory of Parasite and Vector Biology, MOH, China; WHO Collaborating Center for Malaria, Schistosomiasis and Filariasis, Shanghai, 200025, China. caojp@yahoo.com.
authors.forename: Jianping
authors.initials: J
authors.lastname: Cao
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000378:metabolism*; D004283:Dog Diseases / Q000469:parasitology*; D004285:Dogs; D004443:Echinococcosis / Q000469:parasitology / Q000662:veterinary*; D048209:Echinococcus granulosus / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000947:Antigens, Helminth