title: Sphingomonas morindae sp. nov., isolated from Noni (Morinda citrifolia L.) branch.
abstract: Two yellow bacterial strains, designated NBD5(T) and NBD8, isolated from Noni (Morinda citrifolia L.) branch were investigated using a polyphasic taxonomic approach. Cells were Gram-stain-negative, aerobic, non-spore-forming, non-motile and short rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences suggested that the strains were members of a novel species of the genus Sphingomonas, the seven closest neighbours being Sphingomonas oligoaromativorans SY-6(T) (96.9% similarity), Sphingomonas polyaromaticivorans B2-7(T) (95.8%), Sphingomonas yantingensis 1007(T) (94.9%), Sphingomonas sanguinis IFO 13937(T) (94.7%), Sphingomonas ginsenosidimutans Gsoil 1429(T) (94.6%), Sphingomonas wittichii RW1(T) (94.6%) and Sphingomonas formosensis CC-Nfb-2(T) (94.5%). Strains NBD5T and NBD8 had sphingoglycolipid, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylcholine as the major polar lipids, ubiquinone 10 as the predominant respiratory quinone, and sym-homospermidine as the major polyamine. Strains NBD5(T) and NBD8 were clearly distinguished from reference type strains based on phylogenetic analysis, DNA-DNA hybridization, fatty acid composition data analysis, and comparison of a range of physiological and biochemical characteristics. It is evident from the genotypic and phenotypic data that strains NBD5(T) and NBD8 represent a novel species of the genus Sphingomonas, for which the name Sphingomonas morindae sp. nov. is proposed. The type strain is NBD5(T) ( = DSM 29151(T) = KCTC 42183(T) = CICC 10879(T)).
authors.affiliation: China Center of Industrial Culture Collection (CICC), China National Research Institute of Food and Fermentation Industries, Beijing 100015, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: China Center of Industrial Culture Collection (CICC), China National Research Institute of Food and Fermentation Industries, Beijing 100015, PR China.
authors.forename: Su
authors.initials: S
authors.lastname: Yao
authors.affiliation: Microbial Resource Center/Korean Collection for Type Cultures (KCTC), Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Republic of Korea.
authors.forename: Yong-Jae
authors.initials: YJ
authors.lastname: Lee
authors.affiliation: China Center of Industrial Culture Collection (CICC), China National Research Institute of Food and Fermentation Industries, Beijing 100015, PR China.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Cao
authors.affiliation: China Center of Industrial Culture Collection (CICC), China National Research Institute of Food and Fermentation Industries, Beijing 100015, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhai
authors.affiliation: China Center of Industrial Culture Collection (CICC), China National Research Institute of Food and Fermentation Industries, Beijing 100015, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: China Center of Industrial Culture Collection (CICC), China National Research Institute of Food and Fermentation Industries, Beijing 100015, PR China.
authors.forename: Jiaojiao
authors.initials: J
authors.lastname: Su
authors.affiliation: China Center of Industrial Culture Collection (CICC), China National Research Institute of Food and Fermentation Industries, Beijing 100015, PR China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Ge
authors.affiliation: Microbial Resource Center/Korean Collection for Type Cultures (KCTC), Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Republic of Korea.|University of Science and Technology, Yuseong-gu, Daejeon 305-850, Republic of Korea.
authors.forename: Song-Gun
authors.initials: SG
authors.lastname: Kim
authors.affiliation: China Center of Industrial Culture Collection (CICC), China National Research Institute of Food and Fermentation Industries, Beijing 100015, PR China.
authors.forename: Chi
authors.initials: C
authors.lastname: Cheng
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D060026:Endophytes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D032066:Morinda / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013095:Spermidine / Q000031:analogs & derivatives / Q000737:chemistry; D020578:Sphingomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; C007961:sym-homospermidine; D014451:Ubiquinone; C025204:Ubiquinone Q2; D013095:Spermidine