title: Association Between rs2200733 Polymorphism on Chromosome 4q25 and Atrial Fibrillation in a Greek Population.
abstract: BACKGROUND
  Atrial fibrillation (AF) is a common arrhythmia with evidence of genetic susceptibility. The rs2200733 single-nucleotide polymorphism (SNP) in a non-coding region on chromosome 4q25 has been associated with AF. The purpose of this case-control study was to examine the possible association of the rs2200733 polymorphism with AF in the Greek population.
  
  
  METHODS
  A total of 295 individuals, 167 AF patients and 128 controls, were genotyped for the presence of the rs2200733 polymorphism using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLPs) method.
  
  
  RESULTS
  The T/T genotype and the T allele were detected more frequently in patients with AF compared to controls (13.2% vs. 2.3%, p=0.001, and 29.6% vs. 17.9%, p=0.001), suggesting that the rs2200733 polymorphism increases susceptibility to AF in the Greek population. In a multivariate stepwise analysis that included many conventional precipitating factors for AF, T/T genotype and left atrium (LA) diameter were the only independent predictors of AF (OR 1.74, 95% CI: 1.40-2.98, p=0.005, and OR 2.88, 95% CI: 1.835.62, p<0.001, respectively). A trend of association was observed between the T/T genotype and lone AF (p=0.08).
  
  
  CONCLUSIONS
  Our results suggest that SNP rs2200733 confers a significant risk of AF in the Greek population, providing further support to the previously reported association between AF and rs2200733 polymorphism on chromosome 4q25.
authors.affiliation: Department of General Biology, Medical School, Aristotle University of Thessaloniki, Thessaloniki, Greece.
authors.forename: Kallirhoe
authors.initials: K
authors.lastname: Kalinderi
authors.forename: Nikolaos
authors.initials: N
authors.lastname: Fragakis
authors.forename: Konstantinos C
authors.initials: KC
authors.lastname: Koskinas
authors.forename: Demosthenes
authors.initials: D
authors.lastname: Katritsis
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Letsas
authors.forename: Michalis
authors.initials: M
authors.lastname: Efremidis
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Korantzopoulos
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Rossios
authors.forename: Efstathios
authors.initials: E
authors.lastname: Pagourelias
authors.forename: Pinelopi
authors.initials: P
authors.lastname: Giannakopoulou
authors.forename: Melaxrini
authors.initials: M
authors.lastname: Mavroudi
authors.forename: Panagiota
authors.initials: P
authors.lastname: Kyriakou
authors.forename: Vassileios
authors.initials: V
authors.lastname: Skeberis
authors.forename: Paraschos
authors.initials: P
authors.lastname: Geleris
authors.forename: Vassileios
authors.initials: V
authors.lastname: Vassilikos
authors.forename: Liana
authors.initials: L
authors.lastname: Fidani
mesh_terms: D000368:Aged; D001281:Atrial Fibrillation / Q000188:drug therapy / Q000453:epidemiology / Q000235:genetics* / Q000601:surgery; D016022:Case-Control Studies; D002894:Chromosomes, Human, Pair 4; D017548:Echocardiography, Transesophageal / Q000379:methods; D004562:Electrocardiography / Q000379:methods; D015716:Electrocardiography, Ambulatory / Q000379:methods; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006115:Greece / Q000453:epidemiology; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D000097577:Homeobox Protein PITX2
chemical_list: D018398:Homeodomain Proteins; D014157:Transcription Factors; D000097577:Homeobox Protein PITX2