title: Assessment of genetic characteristics ofAconitumgermplasms in Xinjiang Province (China) by RAPD and ISSR markers.
abstract: Aconitumis a medicinal treasure trove that grows extensively on fertile pastures in Xinjiang Province (China); however, its molecular genetic characteristics are still poorly studied. We studiedAconitum kusnezoffiiReichb.,Aconitum soongaricumStapf.,Aconitum carmichaeliiDebx. andAconitum leucostomumWorosch, using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) techniques, to evaluate their genetic relationship and potential medicinal value. Our results showed thatA.kusnezoffiiReichb. andA.soongaricumStapf.have close genetic relationship and cluster together. Polymorphism rates of 97.25% and 98.92% were achieved by using 15 RAPD and 15 ISSR primers, respectively. Based on Nei's gene diversity (H) and Shannon's index (I), the inter-population diversity (Hs) was higher when compared with the intra-population diversity (Hp). Among the threeAconitumpopulations, the coefficient of gene differentiation (Gst) was 0.4358 when evaluated by RAPD and 0.5005 by ISSR. The genetic differentiation among the threeAconitumpopulations was highly significant, suggesting low gene flow (Nm). This was confirmed by the estimates of gene flow (Nm= 0.6473 andNm= 0.4991, based on ISSR and RAPD data, respectively). Comparing the RAPD and ISSR results, the two DNA markers proved similarly effective in the assessment of the genetic characteristics of the studiedAconitumpopulations and could be used for reliable fingerprinting and mapping in studies onAconitumdiversity in view ofAconitumsuitability for development and protection.
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Xinjiang Medical University , Urumqi , Xinjiang , PR China ; Department of Phytochemistry, Affiliated Traditional Chinese Medical Hospital, Xinjiang Medical University , Urumqi , Xinjiang , PR China.
authors.forename: Feicui
authors.initials: F
authors.lastname: Zhao
authors.affiliation: Department of Phytochemistry, Affiliated Traditional Chinese Medical Hospital, Xinjiang Medical University , Urumqi , Xinjiang , PR China.
authors.forename: Jihong
authors.initials: J
authors.lastname: Nie
authors.affiliation: Department of Phytochemistry, Affiliated Traditional Chinese Medical Hospital, Xinjiang Medical University , Urumqi , Xinjiang , PR China.
authors.forename: Muzhi
authors.initials: M
authors.lastname: Chen
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Xinjiang Medical University , Urumqi , Xinjiang , PR China.
authors.forename: Guirong
authors.initials: G
authors.lastname: Wu
keywords: Aconitum; ISSR; RAPD; genetic characteristics