title: Conditioned Media from Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Inhibits Melanogenesis by Promoting Proteasomal Degradation of MITF.
abstract: Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) secrete various beneficial molecules, which have anti-apoptotic activity and cell proliferation. However, the effect of hUCB-MSCs in melanogenesis is largely unclear. In this study, we show that conditioned media (CM) derived from hUCB-MSCs inhibit melanogenesis by regulating microphthalmia-associated transcription factor (MITF) expression via the ERK signalling pathway. Treatment of hUCB-MSC-CM strongly inhibited the alpha-melanocyte stimulating hormone-induced hyperpigmentation in melanoma cells as well as melanocytes. Treatment of hUCB-MSC-CM induced ERK1/2 activation in melanocytes. In addition, inhibition of ERK1/2 suppressed the anti-pigmentation activity of the hUCB-MSC-CM in melanocytes and in vitro artificial skin models. We also found that the expression of MITF was appreciably diminished while expression of phosphorylated MITF, which leads to its proteasomal degradation, was increased in cells treated with hUCB-MSC-CM. These results suggested that hUCB-MSC-CM significantly suppresses melanin synthesis via MITF degradation by the ERK pathway activation.
authors.affiliation: Graduate School of East-West Medical Science, Kyung Hee University, Yongin-si, Gyeonggi-do, Republic of Korea.
authors.forename: Eun Sung
authors.initials: ES
authors.lastname: Kim
authors.affiliation: Biomedical Research Institute, MEDIPOST Co., Ltd., Seongnam-si, Gyeonggi-do, Republic of Korea.
authors.forename: Hong Bae
authors.initials: HB
authors.lastname: Jeon
authors.affiliation: Biomedical Research Institute, MEDIPOST Co., Ltd., Seongnam-si, Gyeonggi-do, Republic of Korea.
authors.forename: Hoon
authors.initials: H
authors.lastname: Lim
authors.affiliation: Graduate School of East-West Medical Science, Kyung Hee University, Yongin-si, Gyeonggi-do, Republic of Korea.
authors.forename: Ji Hyun
authors.initials: JH
authors.lastname: Shin
authors.affiliation: Graduate School of East-West Medical Science, Kyung Hee University, Yongin-si, Gyeonggi-do, Republic of Korea.
authors.forename: So Jung
authors.initials: SJ
authors.lastname: Park
authors.affiliation: Graduate School of East-West Medical Science, Kyung Hee University, Yongin-si, Gyeonggi-do, Republic of Korea.
authors.forename: Yoon Kyung
authors.initials: YK
authors.lastname: Jo
authors.affiliation: Biomedical Research Institute, MEDIPOST Co., Ltd., Seongnam-si, Gyeonggi-do, Republic of Korea.
authors.forename: Wonil
authors.initials: W
authors.lastname: Oh
authors.affiliation: Biomedical Research Institute, MEDIPOST Co., Ltd., Seongnam-si, Gyeonggi-do, Republic of Korea.
authors.forename: Yoon Sun
authors.initials: YS
authors.lastname: Yang
authors.affiliation: Graduate School of East-West Medical Science, Kyung Hee University, Yongin-si, Gyeonggi-do, Republic of Korea.
authors.forename: Dong-Hyung
authors.initials: DH
authors.lastname: Cho
authors.affiliation: Biomedical Research Institute, MEDIPOST Co., Ltd., Seongnam-si, Gyeonggi-do, Republic of Korea.
authors.forename: Ju-Yeon
authors.initials: JY
authors.lastname: Kim
mesh_terms: D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D005312:Fetal Blood / Q000166:cytology; D006801:Humans; D008544:Melanocytes / Q000187:drug effects / Q000502:physiology; D008545:Melanoma / Q000188:drug therapy / Q000473:pathology; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051739:Microphthalmia-Associated Transcription Factor / Q000378:metabolism*; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D012880:Skin Pigmentation / Q000187:drug effects; D000521:alpha-MSH / Q000378:metabolism / Q000494:pharmacology
chemical_list: D017077:Culture Media, Conditioned; C497234:MITF protein, human; D051739:Microphthalmia-Associated Transcription Factor; D000521:alpha-MSH; C535150:MAPK1 protein, human; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D046988:Proteasome Endopeptidase Complex