title: MERFISHing for spatial context.
abstract: A new paper in Science by Zhuang and colleagues introduces multiplexed error-robust FISH (MERFISH). MERFISH extends single-molecule imaging techniques to profile the copy number and localization patterns of thousands of genes, representing a major advance for spatial transcriptomics, with exciting potential applications in immunology.
authors.affiliation: Institute for Medical Engineering & Science & Department of Chemistry, MIT, Cambridge, MA, USA; Ragon Institute of MGH, MIT, & Harvard, Cambridge, MA, USA; Broad Institute of MIT & Harvard, Cambridge, MA, USA.
authors.forename: Alex K
authors.initials: AK
authors.lastname: Shalek
authors.affiliation: New York Genome Center, New York, NY, USA; New York University, Center for Genomics and Systems Biology, New York, NY, USA. Electronic address: rahuls@broadinstitute.org.
authors.forename: Rahul
authors.initials: R
authors.lastname: Satija
mesh_terms: D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis*; D059010:Single-Cell Analysis / Q000379:methods*; D059467:Transcriptome
chemical_list: D012333:RNA, Messenger