title: Expression, Purification and Immunogenic Description of a Hepatitis C Virus Recombinant CoreE1E2 Protein Expressed by Yeast Pichia pastoris.
abstract: BACKGROUND
  Gradual development of a useful vaccine can be the main point in the control and eradication of Hepatitis C virus (HCV) infection. Hepatitis C Virus envelope glycoproteins are considered as the main HCV vaccine candidate.
  
  
  OBJECTIVE
  In this study, the Pichia pastoris expression system was used to express a recombinant HCV CoreE1E2 protein, which consists of Core (269 nt-841nt) E1 (842 nt-1417nt) and E2 (1418 nt-2506nt).
  
  
  METHODS
  By a codon optimization technique based on the P. pastoris expression system, we could increase the rate of recombinant proteins. Moreover, the purified protein can efficiently induce anti-CoreE1E2 antibodies in rabbits, and also by developing a homemade Enzyme-Linked ELISA kit we can detect antibody of HCV Iranian patients with genotype 1a.
  
  
  RESULTS
  In our study, the virus-like particle of rCoreE1E2 with 70 nm size, was shown by Electron microscopy and proved the self-assembly in vitro in a yeast expression system.
  
  
  CONCLUSIONS
  These findings of the present study indicate that the recombinant CoreE1E2 glycoprotein is effective in inducing neutralizing antibodies, and is an influential HCV vaccine candidate.
authors.affiliation: Department of Medical Virology, Iran University of Medical Sciences, Tehran, IR Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Fazlalipour
authors.affiliation: Department of Medical Virology, Iran University of Medical Sciences, Tehran, IR Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Keyvani
authors.affiliation: Department of Medical Virology, Iran University of Medical Sciences, Tehran, IR Iran.
authors.forename: Seyed Hamid Reza
authors.initials: SH
authors.lastname: Monavari
authors.affiliation: Department of Medical Virology, Iran University of Medical Sciences, Tehran, IR Iran.
authors.forename: Hamid Reza
authors.initials: HR
authors.lastname: Mollaie
keywords: Hepatitis C Virus (HCV); Pichia pastoris; Recombinant Core-E1-E2 Glycoprotein; Virus Like Particles