title: Simultaneous determination of five triterpene acids in rat plasma by liquid chromatography-mass spectrometry and its application in pharmacokinetic study after oral administration of Folium Eriobotryae effective fraction.
abstract: Folium Eriobotryae effective fraction (FEA), the extract of Folium Eriobotryae, had been used as anti-hyperglycemia and anti-hyperlipemia medicine in China. A previous study indicated that euscaphic acid, maslinic acid, corosolic acid, oleanolic acid and ursolic acid, the five structurally similar triterpene acids (containing two groups of structural isomers), are the major components of FEA. In the present study, we developed a specific and reliable LC-MS method for simultaneous determination of the five triterpene acids in rat plasma, and further investigated their pharmacokinetic properties after oral administration of FEA. Following a simple sample preparation, chromatographic separation was achieved on a C18 column with a mobile phase composed of methanol-0.1% ammonium acetate (80:20, v/v). Quantification was achieved by monitoring the selected ions at m/z 487.6 for euscaphic acid, m/z 471.5 for maslinic acid and corosolic acid, m/z 455.5 for oleanolic acid and ursolic acid and m/z 469.5 for internal standard. The method was validated to be specific, accurate and precise over the concentration ranges of 10-3000 ng/mL with limits of detections of 5 ng/mL for the five triterpene acids. Finally, the method was successfully applied to the pharmacokinetic study of the five structurally similar triterpene acids in rats after oral administration of FEA.
authors.affiliation: Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, 210046, People's Republic of China.|Jiangsu Province Academy of Traditional Chinese Medicine and Jiangsu Branch of China Academy of Chinese Medical Sciences, Nanjing, 210028, People's Republic of China.
authors.forename: Zhen-Hua
authors.initials: ZH
authors.lastname: Li
authors.affiliation: Jiangsu Province Academy of Traditional Chinese Medicine and Jiangsu Branch of China Academy of Chinese Medical Sciences, Nanjing, 210028, People's Republic of China.
authors.forename: He
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, 210046, People's Republic of China.|Jiangsu Province Academy of Traditional Chinese Medicine and Jiangsu Branch of China Academy of Chinese Medical Sciences, Nanjing, 210028, People's Republic of China.
authors.forename: Xue-Ping
authors.initials: XP
authors.lastname: Cai
authors.affiliation: Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, 210046, People's Republic of China.
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: He
authors.affiliation: Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, 210046, People's Republic of China.|Jiangsu Province Academy of Traditional Chinese Medicine and Jiangsu Branch of China Academy of Chinese Medical Sciences, Nanjing, 210028, People's Republic of China.
authors.forename: Jun-Lei
authors.initials: JL
authors.lastname: Hua
authors.affiliation: Jiangsu Province Academy of Traditional Chinese Medicine and Jiangsu Branch of China Academy of Chinese Medical Sciences, Nanjing, 210028, People's Republic of China.
authors.forename: Jian-Ming
authors.initials: JM
authors.lastname: Ju
authors.affiliation: Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014, China.
authors.forename: Han
authors.initials: H
authors.lastname: Lv
authors.affiliation: Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014, China.
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.affiliation: Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014, China.
authors.forename: Wei-Lin
authors.initials: WL
authors.lastname: Li
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage; D057230:Limit of Detection; D016014:Linear Models; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D014315:Triterpenes / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification / Q000493:pharmacokinetics*
chemical_list: D004365:Drugs, Chinese Herbal; D014315:Triterpenes
keywords: Folium Eriobotryae; liquid chromatography-mass spectrometry; pharmacokinetic; quantitative analysis; triterpene acids