title: [Glutathione-dependent enzymes and glutathione in infertility of men with different body mass].
abstract: OBJECTIVE
  Our aim was to investigate the glutathione-disulfide system of antioxidant defense in men with different body mass index and infertility.
  
  
  METHODS
  60 men with infertility were examined--30 with normal weight and 30 with overweight. 30fertile men were included in the control group. Hemolysate made from red blood cells was used as a materialfor study. The level of reduced (GSH) and oxidized glutathione (GSSG), and glutathione activity was determined by enzyme spectrofluorophotometer SHIMADZU-1501 (Japan).
  
  
  RESULTS
  In infertile men with normal body weight decrease of GSH to 15.35% was combined with a decrease in the activity of enzymes--glutathione reductase (GR) to 53.41%, glutathione peroxidase (GPO) to 51.44%, and increased activity of glutathione-S-transferaze (GST) to 31.52% as compared with the controlgroup. In the group of infertile men with overweight a decrease in the activity of GR, 56.77% and 56.22% at the GPO on the background of a stable level of GST was detected in comparison with fertile men. The concentration of GSH in infertile group decreased by 20.08%, and the concentration of GSSG increased by 20.38%.
  
  
  CONCLUSIONS
  It has been established that imbalance in the glutathione system is the most prominent feature in men with infertility and overweight as compared to infertile men with normal body weight.
authors.forename: L I
authors.initials: LI
authors.lastname: Kolesnikova
authors.forename: O A
authors.initials: OA
authors.lastname: Vanteeva
authors.forename: N A
authors.initials: NA
authors.lastname: Kurashova
authors.forename: B G
authors.initials: BG
authors.lastname: Dashiev
mesh_terms: D000328:Adult; D015992:Body Mass Index; D004542:Ejaculation; D019803:Glutathione Disulfide / Q000097:blood*; D005980:Glutathione Reductase / Q000097:blood*; D006801:Humans; D007248:Infertility, Male / Q000097:blood / Q000209:etiology*; D008297:Male; D050177:Overweight / Q000097:blood / Q000150:complications*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D012661:Semen / Q000378:metabolism
chemical_list: D005980:Glutathione Reductase; D019803:Glutathione Disulfide