title: Cryopreservation of rainbow trout spermatozoa (Onchorhynchus mykiss) using different cryodiluents.
abstract: An experiment was conducted to evaluate the potential of extenders on sperm motility and to delineate the effect of cyodiluent compostion on post thaw motility, fertilization and hatching percentage in rainbow trout (Onchorhynchus mykiss) sperm. The mature males of rainbow trout with an average weight of (340 15) g and average size (28.8 0.6) cm were used. In the trials for testing the suitability of cryoprotectants, DMSO yielded higher motility percentage with extenders Zhang and Liu and 0.6 M Sucrose. Significantly (P <0.05) higher value of % post thaw motility and fertilization was recorded in cryodiluent composition of 8 % DMSO and Zhang and Liu. Additionally, composition of 8 % DMSO with Zhang and Liu led to significant (P <0.05) increase in percentage hatching. Overall, results indicated that the combination of (8 % DMSO + Zhang and Liu) produced best results in terms of percentage post thaw motility, fertilization and hatching.
authors.affiliation: Division of Fish Genetics and Biotechnology, Central Institute of Fisheries Education, Versova, Mumbai-61, India.
authors.forename: P
authors.initials: P
authors.lastname: Makwana Nayan
authors.affiliation: Directorate of Coldwater Fisheries Research, Chhirapani Fish Farm, Champawat, India.
authors.forename: Sanjay K
authors.initials: SK
authors.lastname: Gupta
authors.affiliation: Directorate of Coldwater Fisheries Research, Chhirapani Fish Farm, Champawat, India.
authors.forename: Sathis K
authors.initials: SK
authors.lastname: Srivastva
authors.affiliation: Division of Fish Genetics and Biotechnology, Central Institute of Fisheries Education, Versova, Mumbai-61, India.
authors.forename: Gopal
authors.initials: G
authors.lastname: Krishna
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000378:metabolism*; D004121:Dimethyl Sulfoxide / Q000378:metabolism; D005260:Female; D005306:Fertilization; D008297:Male; D017686:Oncorhynchus mykiss / Q000502:physiology*; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
chemical_list: D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide