title: IL-18 contributes to susceptibility to Leishmania amazonensis infection by macrophage-independent mechanisms.
abstract: We evaluated the role of IL-18 during Leishmania amazonensis infection in C57BL/6 mice, using IL-18KO mice. We showed that IL-18 is involved in susceptibility to L. amazonensis, since IL-18KO mice presented reduced lesions and parasite loads. Because macrophages are the host cells of the parasite, we investigated if macrophages were involved in IL-18-mediated susceptibility to L. amazonensis. We showed that macrophages obtained from WT or IL-18KO responded similarly to L. amazonensis infection. Moreover, we showed that C57BL/6 macrophages do not respond to IL-18, since they do not express IL-18R. Therefore, macrophages are not involved in IL-18-mediated susceptibility to L. amazonensis.
authors.affiliation: Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil; Maryland Pathogen Research Institute, University of Maryland, College Park, MD, USA.
authors.forename: Louisa M A
authors.initials: LM
authors.lastname: Sousa
authors.affiliation: Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Matheus B H
authors.initials: MB
authors.lastname: Carneiro
authors.affiliation: Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Liliane M
authors.initials: LM
authors.lastname: Dos Santos
authors.affiliation: Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Caio Cotta
authors.initials: CC
authors.lastname: Natale
authors.affiliation: Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Magda E
authors.initials: ME
authors.lastname: Resende
authors.affiliation: Maryland Pathogen Research Institute, University of Maryland, College Park, MD, USA.
authors.forename: David M
authors.initials: DM
authors.lastname: Mosser
authors.affiliation: Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil. Electronic address: lqvieira@icb.ufmg.br.
authors.forename: Leda Q
authors.initials: LQ
authors.lastname: Vieira
mesh_terms: D000818:Animals; D004198:Disease Susceptibility; D020382:Interleukin-18 / Q000235:genetics / Q000276:immunology*; D007891:Leishmania / Q000276:immunology*; D007896:Leishmaniasis / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D008264:Macrophages / Q000276:immunology / Q000473:pathology; D051379:Mice; D018345:Mice, Knockout; D053724:Receptors, Interleukin-18 / Q000235:genetics / Q000276:immunology
chemical_list: D020382:Interleukin-18; D053724:Receptors, Interleukin-18
keywords: IL-18; Leishmania amazonensis; Leishmaniasis; Macrophage