title: Effect of BMP-6 on development and maturation of mouse preantral folliclesin vitro.
abstract: The aim of this study was to investigate the effect and mechanism of bone morphogenetic protein-6 (BMP-6) on the growth and maturation of mouse folliclesin vitro. Preantral follicles isolated from mice were incubated with recombinant human BMP-6 (rhBMP-6) before analysis. BMP-6 expression was detected by immunofluorescence and western blot. Maturation of oocytes was observed microscopically. Estradiol (E2) and progesterone (P4) levels were measured by enzyme-linked immunosorbent assay. Expression of steroidogenesis-related genes was detected by reverse transcription quantitative polymerase chain reaction. There was a marked increase in the preantral follicles maturation in cells incubated with 50 ng/mL of rhBMP-6 for eight days, compared with the control. The levels of E2, P4and steroidogenesis-related genes were also significantly increased in granulosa cells and theca cells cultured for 6, 10 and 11 days, respectively. Conversely, the preantral follicle maturing rate was remarkably decreased in cells incubated with 50 ng/mL of rhBMP-6 for day 11, accompanied with reduction in E2, P4levels and steroidogenesis-related genes levels. Meanwhile, compared with the control, the maturing rate was not significantly different in cells incubated with 100 ng/mL of rhBMP-6 for day 8 or day 11. However, the E2levels and its relevant regulation gene expression all increased significantly, while the P4content and its relevant regulation gene expression decreased. The results indicate that BMP-6 can promote the maturation of preantral folliclesin vitroin a concentration and time-dependent manner and may play a role in the regulation of steroid hormone synthesis and/or secretion.
authors.affiliation: Department of Histology and Embryology, Medical College, Nantong University , Nantong , P.R. China.
authors.forename: Xiyan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Neurology, Second Clinical Hospital, Jilin University , Changchun , P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Su
authors.affiliation: Department of Histology and Embryology, Medical College, Jilin Medical University , Jilin , P.R. China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Pan
authors.affiliation: Department of Histology and Embryology, Medical College, Nantong University , Nantong , P.R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Yao
authors.affiliation: Department of Histology and Embryology, Medical College, Jilin Medical University , Jilin , P.R. China.
authors.forename: Zhixin
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Reproduction, Reproductive Medicine Center of the Affiliated Hospital of Jining Medical College , Jining , P.R. China.
authors.forename: Xuenan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Histology and Embryology, Medical College, Nantong University , Nantong , P.R. China.
authors.forename: Bangsheng
authors.initials: B
authors.lastname: Xu
keywords: BMP-6; estradiol; preantral follicles; progesterone; steroid hormone; synthetic regulation enzyme