title: [Protective effect of curcumin derivative B06 on kidney of type 2 diabetic rats].
abstract: OBJECTIVE
  To observe the effect and mechanism of curcumin derivative B06 on kidney from rats with hyperlipidemia and type 2 diabetes.
  
  
  METHODS
  Thirty five male SD rats were randomly divided into five groups(n = 7): the normal control group, high-fat group, high-fat + B06-treatd group, diabetic group, diabetic + B06-treated group. After fed with high-fat diet for 4 weeks, the later two groups were in- jected with streptozotocin intraperitoneally to induce type 2 diabetes mellitus. B06-treated groups were given B06 by gavage at a dosage of 0.2 mg/kg . d for 8 weeks. After the treatment, the serum creatinine, blood urea nitrogen and uric acid were detected biochemically, the morphology of kidney was observed with light and transmission electron microscopy, the expression of collagen fibers was observed with Masson staining, the protein expression of collogen IV and fibronectin in kidney were determined by Immunohistochemistry.
  
  
  RESULTS
  It was showed that the levels of the serum creatinine and blood urea nitrogen elevated significantly in diabetic group. In high-fat and diabetic groups, increased glomerular mesangial matrix and collagen fiber and thicken glomerular basal membrane were observed under light microscopy, swelling and fusion of foot process were found under electron microscope; increased green matrix within glomeruli was observed under Masson staining. collogen IV and fibronectin protein expression were significantly enhanced in high-fat group and diabetic group. After B06's intervention, the levels of serum creatinine and blood urea nitrogen were decreased in diabetic groups, the morphological change of kidney was obviously relieved, Collogen IV and fibronectin protein expression reduced.
  
  
  CONCLUSIONS
  Curcumin derivative B06 exerts a protective effect on kidney in type 2 diabetic rats, reduced expressions of collogen IV and fibronectin, inhibition of the accumulation of extracellular matrix and glomerular mesangial proliferation, and then prevention of renal fibrosis may be the mechanism.
authors.forename: Cong-cong
authors.initials: CC
authors.lastname: Zeng
authors.forename: Xi
authors.initials: X
authors.lastname: Liu
authors.forename: Wang-wang
authors.initials: WW
authors.lastname: Liu
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.forename: Jin-guo
authors.initials: JG
authors.lastname: Cheng
authors.forename: San-mei
authors.initials: SM
authors.lastname: Chen
authors.forename: Guo-rong
authors.initials: GR
authors.lastname: Chen
mesh_terms: D000818:Animals; D001806:Blood Urea Nitrogen; D024141:Collagen Type IV / Q000378:metabolism; D003404:Creatinine / Q000097:blood; D003474:Curcumin / Q000494:pharmacology*; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000188:drug therapy*; D003924:Diabetes Mellitus, Type 2; D004365:Drugs, Chinese Herbal; D005353:Fibronectins / Q000378:metabolism; D007668:Kidney / Q000378:metabolism / Q000503:physiopathology; D007674:Kidney Diseases / Q000188:drug therapy*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013311:Streptozocin; D014527:Uric Acid / Q000097:blood
chemical_list: D024141:Collagen Type IV; D004365:Drugs, Chinese Herbal; D005353:Fibronectins; D014527:Uric Acid; D013311:Streptozocin; D003404:Creatinine; D003474:Curcumin