title: The chloroplast genome sequence of an important medicinal plant Dioscorea nipponica.
abstract: Dioscorea nipponica is an important medicinal plant belonging to Dioscoreaceae, a family which is vital for the evolution of monocotyledon. In the present study, the nucleotide sequence of the D. nipponica chloroplast genome was determined. It was an AT-rich (63.3%) chloroplast genome with 152,946 bp in length, containing a pair of 23,113 bp inverted repeats, which were separated by a large and a small single copy region of 83,557 bp and 23,064 bp in length, respectively. It encodes 120 unique genes, including 89 protein-coding genes, 27 tRNA genes and 4 rRNA genes. The predicted gene-coding regions covered 58.7% of the genome sequences. Ten genes contained one intron, while two genes had two introns. Phylogenetic analyses showed the present chloroplast genome can be used as a potential supper barcode to distinguish D. nipponica from its closely related species. Furthermore, the chloroplast genome provides a molecular base for the next investigation on this important medicinal species.
authors.affiliation: a College of Pharmacy, Hubei University of Chinese Medicine , Wuhan , China .|b Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences , Beijing , China , and.
authors.forename: Lan
authors.initials: L
authors.lastname: Wu
authors.affiliation: c Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College , Beijing , China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.affiliation: b Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences , Beijing , China , and.
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.affiliation: b Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences , Beijing , China , and.
authors.forename: Chi
authors.initials: C
authors.lastname: Song
authors.affiliation: a College of Pharmacy, Hubei University of Chinese Medicine , Wuhan , China .
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.affiliation: a College of Pharmacy, Hubei University of Chinese Medicine , Wuhan , China .|b Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences , Beijing , China , and.
authors.forename: Shilin
authors.initials: S
authors.lastname: Chen
authors.affiliation: b Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences , Beijing , China , and.
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
mesh_terms: D020856:AT Rich Sequence / Q000235:genetics; D001483:Base Sequence; D018742:DNA, Chloroplast / Q000235:genetics*; D027742:Dioscorea / Q000145:classification / Q000235:genetics*; D054628:Genome, Chloroplast / Q000235:genetics*; D010802:Phylogeny; D010946:Plants, Medicinal / Q000145:classification / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D018742:DNA, Chloroplast; D012335:RNA, Ribosomal; D012343:RNA, Transfer
keywords: Chloroplast genome; Dioscorea; Dioscorea nipponica; medicinal plant