title: Streptococcus gallolyticus Pil3 Pilus Is Required for Adhesion to Colonic Mucus and for Colonization of Mouse Distal Colon.
abstract: Streptococcus gallolyticus is an increasing cause of bacteremia and infective endocarditis in the elderly. Several epidemiological studies have associated the presence of this bacterium with colorectal cancer. We have studied the interaction of S. gallolyticus with human colonic cells. S. gallolyticus strain UCN34, adhered better to mucus-producing cells such as HT-29-MTX than to the parental HT-29 cells. Attachment to colonic mucus is dependent on the pil3 pilus operon, which is heterogeneously expressed in the wild-type UCN34 population. We constructed a pil3 deletion mutant in a Pil3 overexpressing variant (Pil3+) and were able to demonstrate the role of Pil3 pilus in binding to colonic mucus. Importantly, we showed that pil3 deletion mutant was unable to colonize mice colon as compared to the isogenic Pil3+ variant. Our findings establish for the first time a murine model of intestinal colonization by S. gallolyticus.
authors.affiliation: Department of Microbiology, Biology of Gram-Positive Pathogens Unit, Institut Pasteur Department of Microbiology, Centre National de la Recherche Scientifique (CNRS) ERL3526.
authors.forename: Mariana
authors.initials: M
authors.lastname: Martins
authors.affiliation: Department of Cell Biology and Infection, Molecular Microbial Pathogenesis Unit, Institut Pasteur, Paris.
authors.forename: Laetitia
authors.initials: L
authors.lastname: Aymeric
authors.affiliation: Department of Microbiology, Biology of Gram-Positive Pathogens Unit, Institut Pasteur Department of Microbiology, Centre National de la Recherche Scientifique (CNRS) ERL3526.
authors.forename: Laurence
authors.initials: L
authors.lastname: du Merle
authors.affiliation: Department of Microbiology, Biology of Gram-Positive Pathogens Unit, Institut Pasteur Department of Microbiology, Centre National de la Recherche Scientifique (CNRS) ERL3526.
authors.forename: Camille
authors.initials: C
authors.lastname: Danne
authors.affiliation: University Lille Nord, USTL, UGSF, Villeneuve d'Ascq, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Robbe-Masselot
authors.affiliation: Department of Microbiology, Biology of Gram-Positive Pathogens Unit, Institut Pasteur Department of Microbiology, Centre National de la Recherche Scientifique (CNRS) ERL3526.
authors.forename: Patrick
authors.initials: P
authors.lastname: Trieu-Cuot
authors.affiliation: Department of Cell Biology and Infection, Molecular Microbial Pathogenesis Unit, Institut Pasteur, Paris.
authors.forename: Philippe
authors.initials: P
authors.lastname: Sansonetti
authors.affiliation: Department of Microbiology, Biology of Gram-Positive Pathogens Unit, Institut Pasteur Department of Microbiology, Centre National de la Recherche Scientifique (CNRS) ERL3526.
authors.forename: Shaynoor
authors.initials: S
authors.lastname: Dramsi
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001422:Bacterial Adhesion; D002460:Cell Line; D003106:Colon / Q000382:microbiology*; D004847:Epithelial Cells / Q000382:microbiology*; D036781:Fimbriae Proteins / Q000235:genetics; D010861:Fimbriae, Bacterial / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D015870:Gene Expression; D005798:Genes, Bacterial; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009093:Mucus / Q000378:metabolism / Q000382:microbiology*; D009876:Operon; D013290:Streptococcal Infections / Q000382:microbiology*; D013291:Streptococcus / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D018829:Adhesins, Bacterial; D036781:Fimbriae Proteins
keywords: Pil3 pilus; S. bovis; S. gallolyticus; adhesion; colon; colonization; mucins