title: Induction of forkhead box M1 (FoxM1) by EGF through ERK signaling pathway promotes trophoblast cell invasion.
abstract: Successful placentation depends on the proper invasion of extravillous trophoblast (EVT) cells into maternal tissues. Previous reports have demonstrated that FoxM1 is oncogenic and plays important roles in angiogenesis, invasion, and metastasis. However, little is known about the roles of FoxM1 in the invasion of EVT cells. EGF, as a growth factor (epidermal growth factor), has been studied extensively in reproduction. JAR cells are a reliable model for studying early invasive trophoblast regulation. We have observed the relationship between EGF and FoxM1 in JAR cells by using specific inhibitors for the intervention in and study of potential signal pathways. We have also tested the ability of JAR cells to be influenced by the expression of FoxM1. Our data indicate that EGF promotes FoxM1 expression through the ERK signal pathway. Over-FoxM1 expression upregulates the ability of JAR cells to migrate and invade and vice versa. Our investigation of FoxM1 should provide new insights into the molecular mechanisms of EVT invasion.
authors.affiliation: Department of Biochemistry and Molecular Biology, Dalian Medical University, 116044, Dalian, Liaoning, People's Republic of China.
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Department of Biochemistry and Molecular Biology, Dalian Medical University, 116044, Dalian, Liaoning, People's Republic of China.
authors.forename: Dan
authors.initials: D
authors.lastname: Cui
authors.affiliation: Department of Biochemistry and Molecular Biology, Dalian Medical University, 116044, Dalian, Liaoning, People's Republic of China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Sui
authors.affiliation: Department of Biochemistry and Molecular Biology, Dalian Medical University, 116044, Dalian, Liaoning, People's Republic of China.
authors.forename: Yuefei
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Dalian Medical University, 116011, Dalian, Liaoning, People's Republic of China.
authors.forename: Ningning
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry and Molecular Biology, Dalian Medical University, 116044, Dalian, Liaoning, People's Republic of China.
authors.forename: Yanni
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Biochemistry and Molecular Biology, Dalian Medical University, 116044, Dalian, Liaoning, People's Republic of China.
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Biochemistry and Molecular Biology, Dalian Medical University, 116044, Dalian, Liaoning, People's Republic of China. yingkong@dlmedu.edu.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Kong
mesh_terms: D000818:Animals; D002460:Cell Line; D002465:Cell Movement / Q000235:genetics / Q000502:physiology*; D049109:Cell Proliferation / Q000502:physiology; D004815:Epidermal Growth Factor / Q000378:metabolism*; D005260:Female; D000072278:Forkhead Box Protein M1; D051858:Forkhead Transcription Factors / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D020935:MAP Kinase Signaling System / Q000502:physiology*; D008297:Male; D051379:Mice; D014327:Trophoblasts / Q000378:metabolism*
chemical_list: D000072278:Forkhead Box Protein M1; D051858:Forkhead Transcription Factors; C491524:Foxm1 protein, mouse; D004815:Epidermal Growth Factor
keywords: ERK; Embryo; FoxM1; Implantation; JAR cells; Mouse