title: Identification of Drug Targets in Helicobacter pylori by in silico Analysis: Possible Therapeutic Implications for Gastric cancer.
abstract: Helicobacter pylori colonize stomach, inducing gastritis, ulcers and gastric cancer. Drugs are used to relieve pain, but not H. pylori infections. Hence, there is a need for discovery of drug targets and drugs for H. pylori. An objective of this current study is to identify drug targets for H. pylori. RAST was used to compare genomes of 23 H. pylori strains with Homo sapiens sapiens, other Helicobacter species (H. acinonychis, H. hepaticus, H. mustalae) and among them, to identify 13471 unique genes. Bacterial genes which are non-homologous to humans and essential for pathogen are identified using BLASTp. Later, 29 potential drug targets were identified by subjecting these genes to property analysis. Eleven of the 29 drug targets are already experimentally validated, lending credence to our approach. These methods have enabled rapid identification of drug targets with possible therapeutic implications for gastric cancer.
authors.forename: Deepthi
authors.initials: D
authors.lastname: Nammi
authors.forename: Ravi C P K
authors.initials: RC
authors.lastname: Srimath-Tirumala-Peddinti
authors.affiliation: Department of Bioinformatics, School of Life Sciences, GITAM Institute of Science, GITAM University, Rushikonda, Visakhapatnam- 530045. AP, India. nrneelapu@gmail.com.
authors.forename: Nageswara Rao R
authors.initials: NR
authors.lastname: Neelapu
mesh_terms: D000970:Antineoplastic Agents / Q000627:therapeutic use*; D016480:Helicobacter pylori / Q000187:drug effects*; D013274:Stomach Neoplasms / Q000188:drug therapy*
chemical_list: D000970:Antineoplastic Agents