title: Simple Methods for Tracking Stem Cells with (64)Cu-Labeled DOTA-hexadecyl-benzoate.
abstract: The purpose of this study was to evaluate (64)Cu-labeled hexadecyl-1,4,7,10-tetraazacyclododecane-tetraacetic acid-benzoate ((64)Cu-DOTA-HB) (1) as positron emission tomography (PET) radiotracer for stem cell imaging. Hexadecyl-DOTA-benzoate (DOTA-HB) (2) was efficiently labeled with (64)Cu (>99%), and cell labeling efficiency with adipose-derived stem cells (ADSCs) was over 50%. Labeling with 1 did not compromise cell viability. In the PET imaging, intramuscularly transplanted 1-labeled ADSCs were monitored for 18 h in normal rat heart. These results indicate that 1 can be utilized as a promising radiotracer for monitoring of transplanted stem cells.
authors.affiliation: Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences , Seoul 139-706, Republic of Korea ; School of Life Sciences and Biotechnology, Korea University , Seoul 136-701, Republic of Korea.
authors.forename: Min Hwan
authors.initials: MH
authors.lastname: Kim
authors.affiliation: Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences , Seoul 139-706, Republic of Korea.
authors.forename: Sang-Keun
authors.initials: SK
authors.lastname: Woo
authors.affiliation: Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences , Seoul 139-706, Republic of Korea.
authors.forename: Kwang Il
authors.initials: KI
authors.lastname: Kim
authors.affiliation: Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences , Seoul 139-706, Republic of Korea.
authors.forename: Tae Sup
authors.initials: TS
authors.lastname: Lee
authors.affiliation: School of Life Sciences and Biotechnology, Korea University , Seoul 136-701, Republic of Korea.
authors.forename: Chan Wha
authors.initials: CW
authors.lastname: Kim
authors.affiliation: Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences , Seoul 139-706, Republic of Korea.
authors.forename: Joo Hyun
authors.initials: JH
authors.lastname: Kang
authors.affiliation: Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences , Seoul 139-706, Republic of Korea.
authors.forename: Byung Il
authors.initials: BI
authors.lastname: Kim
authors.affiliation: Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences , Seoul 139-706, Republic of Korea.
authors.forename: Sang Moo
authors.initials: SM
authors.lastname: Lim
authors.affiliation: Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences , Seoul 139-706, Republic of Korea.
authors.forename: Kyo Chul
authors.initials: KC
authors.lastname: Lee
authors.affiliation: Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences , Seoul 139-706, Republic of Korea.
authors.forename: Yong Jin
authors.initials: YJ
authors.lastname: Lee
keywords: Adipose-derived stem cells; PET; copper-64; hexadecyl benzoate; radiotracer