title: An Efficient PEG/CaCl₂-Mediated Transformation Approach for the Medicinal Fungus Wolfiporia cocos.
abstract: Sclerotia of Wolfiporia cocos are of medicinal and culinary value. The genes and molecular mechanisms involved in W. cocos sclerotial formation are poorly investigated because of the lack of a suitable and reproducible transformation system for W. cocos. In this study, a PEG/ CaCl₂-mediated genetic transformation system for W. cocos was developed. The promoter Pgpd from Ganoderma lucidum effectively drove expression of the hygromycin B phosphotransferase gene in W. cocos, and approximately 30 transformants were obtained per 10 μg DNA when the protoplast suspension density was 10(6) protoplasts/ml. However, no transformants were obtained under the regulation of the PtrpC promoter from Aspergillus nidulans.
authors.affiliation: College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, P.R. China.
authors.forename: Qiao
authors.initials: Q
authors.lastname: Sun
authors.affiliation: Institute for Interdisciplinary Research, Jianghan University, Wuhan 430056, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.affiliation: College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, P.R. China.
authors.forename: Juan
authors.initials: J
authors.lastname: Zhao
authors.affiliation: College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, P.R. China.
authors.forename: Jia
authors.initials: J
authors.lastname: Song
authors.affiliation: College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, P.R. China.
authors.forename: Fang
authors.initials: F
authors.lastname: Peng
authors.affiliation: College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, P.R. China.
authors.forename: Shaopeng
authors.initials: S
authors.lastname: Zhang
authors.affiliation: College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, P.R. China.
authors.forename: Yonglian
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, P.R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.affiliation: College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, P.R. China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Zhu
mesh_terms: D000935:Antifungal Agents / Q000378:metabolism; D001233:Aspergillus nidulans / Q000235:genetics; D002122:Calcium Chloride / Q000378:metabolism*; D055453:Coriolaceae / Q000187:drug effects* / Q000235:genetics*; D018014:Gene Transfer Techniques; D005827:Genetics, Microbial / Q000379:methods*; D006921:Hygromycin B / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism; D011092:Polyethylene Glycols / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D020940:Reishi / Q000235:genetics; D012641:Selection, Genetic; D014170:Transformation, Genetic
chemical_list: D000935:Antifungal Agents; D011092:Polyethylene Glycols; D006921:Hygromycin B; D017853:Phosphotransferases (Alcohol Group Acceptor); C063242:hygromycin-B kinase; D002122:Calcium Chloride
keywords: Genetic transformation; Wolfiporia cocos; medicinal and culinary value; promoter