title: Preparation of Lentinula edodes polysaccharide-calcium complex and its immunoactivity.
abstract: Polysaccharide is a major bioactive component of mushrooms. In this study, for the first time, starting from a new Lentinula edodes polysaccharide L2, we prepared a novel L2-calcium complex and the process was optimized. Scanning electron microscopy and Fourier Transform infrared spectrometry were used for characterization. The immunostimulating activities of L2 and L2-calcium complex were measured by enhancing the production of two cytokines TNF-α and IL-6 in RAW264.7 cells. While L2-calcium complex significantly stimulates the secretions of TNF-α and IL-6 compared with the control, complex with calcium ion decreased the secretion of them. These facts indicate that calcium ion can modulate immune stimulating activity of Lentinula edodes polysaccharide L2.
authors.affiliation: a College of Light Industry and Food Sciences , South China University of Technology , Guangzhou , China.
authors.forename: Yujiao
authors.initials: Y
authors.lastname: Cui
authors.forename: Huidan
authors.initials: H
authors.lastname: Yan
authors.forename: Xuewu
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002118:Calcium / Q000737:chemistry*; D002461:Cell Line, Transformed; D062610:Fungal Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007155:Immunologic Factors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D015850:Interleukin-6 / Q000096:biosynthesis / Q000378:metabolism; D008262:Macrophage Activation / Q000187:drug effects* / Q000276:immunology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D020942:Shiitake Mushrooms / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000378:metabolism
chemical_list: D062610:Fungal Polysaccharides; D007155:Immunologic Factors; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D002118:Calcium
keywords: Lentinula edodes; calcium supplement; immuno stimulator; polysaccharide–calcium complex; preparation