title: Dexterous robotic manipulation of alert adult Drosophila for high-content experimentation.
abstract: We present a robot that enables high-content studies of alert adult Drosophila by combining operations including gentle picking; translations and rotations; characterizations of fly phenotypes and behaviors; microdissection; or release. To illustrate, we assessed fly morphology, tracked odor-evoked locomotion, sorted flies by sex, and dissected the cuticle to image neural activity. The robot's tireless capacity for precise manipulations enables a scalable platform for screening flies' complex attributes and behavioral patterns.
authors.affiliation: James H. Clark Center, Stanford University, Stanford, California, USA.|Howard Hughes Medical Institute, Stanford University, Stanford, California, USA.|CNC Program, Stanford University, Stanford, California, USA.
authors.forename: Joan
authors.initials: J
authors.lastname: Savall
authors.affiliation: James H. Clark Center, Stanford University, Stanford, California, USA.|Centre for Intelligent Signal and Imaging Research, Universiti Teknologi Petronas, Perak, Malaysia.
authors.forename: Eric Tatt Wei
authors.initials: ETW
authors.lastname: Ho
authors.affiliation: James H. Clark Center, Stanford University, Stanford, California, USA.
authors.forename: Cheng
authors.initials: C
authors.lastname: Huang
authors.affiliation: James H. Clark Center, Stanford University, Stanford, California, USA.|CNC Program, Stanford University, Stanford, California, USA.
authors.forename: Jessica R
authors.initials: JR
authors.lastname: Maxey
authors.affiliation: James H. Clark Center, Stanford University, Stanford, California, USA.|Howard Hughes Medical Institute, Stanford University, Stanford, California, USA.|CNC Program, Stanford University, Stanford, California, USA.
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Schnitzer
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D001696:Biomechanical Phenomena; D004331:Drosophila melanogaster / Q000033:anatomy & histology / Q000502:physiology*; D005260:Female; D057166:High-Throughput Screening Assays; D008297:Male; D010641:Phenotype; D012371:Robotics; D012399:Rotation