title: Primary Myofibroblasts Maintain Short-Term Viability following Submucosal Injection in Syngeneic, Immune-Competent Mice Utilizing Murine Colonoscopy.
abstract: The myofibroblast is an important stromal cell of the gastrointestinal tract. Current in vitro and in vivo models either do not accurately recreate stromal-epithelial interactions or are not specific to myofibroblasts. We sought to create an animal model that would allow the study of myofibroblast-epithelial interactions. We isolated and cultured colonic myofibroblasts from FVB mice. Cells were α-SMA and vimentin positive but desmin negative on immunoblot analysis. We injected the myofibroblasts into the colonic submucosa of syngeneic adult mice (n = 8) via a miniendoscopic system. We then isolated green fluorescent protein (GFP) positive colonic myofibroblasts from C57BL/6-Tg(CAG-EGFP)1Osb/J mice and injected them into the colonic lamina propria of C57BL/6J mice at 1x10(5) (n = 14), 1x10(6) (n = 9), or 5x10(6) cells/mL (n = 4). A subset of mice were injected with serum-free media and ink without cells (n = 3). Mice underwent repeat endoscopy and euthanasia one or 7 days after injection. Colons were isolated and either fixed in 10% formalin or the inked sites were individually excised and lysed for DNA. We assessed the injection sites via histology and immunohistochemical stains for α-SMA and GFP. We used qPCR to quantify GFP DNA transcripts at the lamina propria injection sites. Submucosal injection of myofibroblasts resulted in the formation of a subepithelial wheal on endoscopy, which persisted to day 7. Myofibroblasts injected either into the submucosa or lamina propria maintained viability on post-injection day 7 as evidenced by positive α-SMA staining. qPCR of lamina propria injections showed a dose-dependent increase in GFP DNA transcripts on post-injection day 1, whereas the number of transcripts on day 7 was equivalent for the concentrations injected. We demonstrate short-term survival of primary cultured colonic myofibroblasts in syngeneic mice. This may prove to be a valuable model for studying the role of myofibroblasts in states of health and disease.
authors.affiliation: Department of Surgery, UCLA David Geffen School of Medicine, Los Angeles, California, United States of America.
authors.forename: Hassan A
authors.initials: HA
authors.lastname: Khalil
authors.affiliation: Department of Surgery, UCLA David Geffen School of Medicine, Los Angeles, California, United States of America.
authors.forename: Nan Ye
authors.initials: NY
authors.lastname: Lei
authors.affiliation: Department of Surgery, UCLA David Geffen School of Medicine, Los Angeles, California, United States of America.
authors.forename: Wenxian
authors.initials: W
authors.lastname: Nie
authors.affiliation: Department of Pathology & Laboratory Medicine, VA Greater Los Angeles Health System, Los Angeles, California, United States of America.
authors.forename: Michael S
authors.initials: MS
authors.lastname: Lewis
authors.affiliation: Department of Surgery, UCLA David Geffen School of Medicine, Los Angeles, California, United States of America; Department of Surgery, VA Greater Los Angeles Health System, Los Angeles, California, United States of America.
authors.forename: Matthias G
authors.initials: MG
authors.lastname: Stelzner
authors.affiliation: Division of Gastroenterology and Nutrition, Department of Pediatrics, University of California Los Angeles, Los Angeles, California, United States of America.
authors.forename: Martín G
authors.initials: MG
authors.lastname: Martín
authors.affiliation: Department of Surgery, UCLA David Geffen School of Medicine, Los Angeles, California, United States of America.
authors.forename: James C Y
authors.initials: JC
authors.lastname: Dunn
authors.affiliation: Department of Surgery, Tufts Medical Center, Boston, Massachusetts, United States of America.
authors.forename: James
authors.initials: J
authors.lastname: Yoo
mesh_terms: D000818:Animals; D002470:Cell Survival; D003106:Colon / Q000166:cytology; D003113:Colonoscopy; D005260:Female; D007121:Immunocompetence; D007267:Injections; D007413:Intestinal Mucosa / Q000166:cytology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D058628:Myofibroblasts / Q000166:cytology*; D013997:Time Factors