title: Protection against live rotavirus challenge in mice induced by parenteral and mucosal delivery of VP6 subunit rotavirus vaccine.
abstract: Live oral rotavirus (RV) vaccines are part of routine childhood immunization but are associated with adverse effects, particularly intussusception. We have developed a non-live combined RV - norovirus (NoV) vaccine candidate consisting of human RV inner-capsid rVP6 protein and NoV virus-like particles. To determine the effect of delivery route on induction of VP6-specific protective immunity, BALB/c mice were administered a vaccine containing RV rVP6 intramuscularly, intranasally or a combination of both, and challenged with murine RV. At least 65 % protection against RV shedding was observed regardless of delivery route. The levels of post-challenge serum VP6-specific IgA titers correlated with protection.
authors.affiliation: Vaccine Research Center, University of Tampere Medical School, Biokatu 10, 33520, Tampere, Finland.
authors.forename: Suvi
authors.initials: S
authors.lastname: Lappalainen
authors.forename: Ana Ruth
authors.initials: AR
authors.lastname: Pastor
authors.forename: Maria
authors.initials: M
authors.lastname: Malm
authors.forename: Vanessa
authors.initials: V
authors.lastname: López-Guerrero
authors.forename: Fernando
authors.initials: F
authors.lastname: Esquivel-Guadarrama
authors.forename: Laura A
authors.initials: LA
authors.lastname: Palomares
authors.forename: Timo
authors.initials: T
authors.lastname: Vesikari
authors.forename: Vesna
authors.initials: V
authors.lastname: Blazevic
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D036022:Capsid Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D012401:Rotavirus / Q000235:genetics / Q000276:immunology*; D012400:Rotavirus Infections / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D022243:Rotavirus Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D007074:Immunoglobulin G; D022243:Rotavirus Vaccines; C061332:VP6 protein, Rotavirus