title: Quantitation of Chloramphenicol and Nitrofuran Metabolites in Aquaculture Products Using Microwave-Assisted Derivatization, Automated SPE, and LC-MS/MS.
abstract: This paper describes a rapid and robust method utilizing microwave-assisted derivatization, automated SPE, and LC-MS/MS for the quantitation and confirmation of chloramphenicol (CAP) and nitrofuran metabolites in various aquaculture matrixes. The use of equipment presented in this work allowed extractions to be completed on average within 6 h, with quantitation accuracy ranging from 89 to 107% and RSD≤8.3%. The demonstrated detection limits for all the nitrofuran metabolites of interest in three different matrixes were ≤0.06 ng/g, with a quantitation limit of ≤0.2 ng/g. Additionally, the method exhibited a CAP detection limit for all matrixes≤0.01 ng/g and an LOQ of ≤0.03 ng/g.
authors.affiliation: U.S. Food and Drug Administration, Office of Regulatory Affairs, Arkansas Regional Laboratory, 3900 NCTR Rd, Jefferson, AR 72079, USA.
authors.forename: Brian T
authors.initials: BT
authors.lastname: Veach
authors.forename: Chris A
authors.initials: CA
authors.lastname: Baker
authors.forename: John H
authors.initials: JH
authors.lastname: Kibbey
authors.forename: Andrew
authors.initials: A
authors.lastname: Fong
authors.forename: Bryanna J
authors.initials: BJ
authors.lastname: Broadaway
authors.forename: Connie P
authors.initials: CP
authors.lastname: Drake
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D017756:Aquaculture; D003400:Astacoidea / Q000378:metabolism; D002397:Catfishes / Q000378:metabolism; D002701:Chloramphenicol / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005396:Fish Products / Q000032:analysis*; D057230:Limit of Detection; D008872:Microwaves; D009581:Nitrofurans / Q000032:analysis*; D033561:Penaeidae / Q000378:metabolism; D012015:Reference Standards; D015203:Reproducibility of Results; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D000900:Anti-Bacterial Agents; D009581:Nitrofurans; D002701:Chloramphenicol