title: The mechanism of uranium transformation from U(VI) into nano-uramphite by two indigenous Bacillus thuringiensis strains.
abstract: The mechanism of uranium transformation from U(VI) into nano-uramphite by two indigenous Bacillus thuringiensis strains was investigated in the present work. Our data showed that the bacteria isolated from uranium mine possessed highly accumulation ability to U(VI), and the maximum accumulation capacity was around 400 mg U/g biomass (dry weight). X-ray powder diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FT-IR) analyzes indicated that the U(VI) was adsorbed on the bacterial surface firstly through coordinating with phosphate, CH2 and amide groups, and then needle-like amorphous uranium compounds were formed. With the extension of time, the extracellular crystalline substances were disappeared, but some particles were appeared in the intracellular region, and these particles were characterized as tetragonal-uramphite. Moreover, the disrupted experiment indicated that the cell-free extracts had better uranium-immobilization ability than cell debris. Our findings provided the understanding of the uranium transformation process from amorphous uranium to crystalline uramphite, which would be useful in the regulation of uranium immobilization process.
authors.affiliation: Key Lab of Biopesticide and Chemical Biology, Fujian Agriculture and Forestry University, Ministry of Education & Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Fuzhou, Fujian 350002, PR China; Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, PR China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Pan
authors.affiliation: Key Lab of Biopesticide and Chemical Biology, Fujian Agriculture and Forestry University, Ministry of Education & Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Fuzhou, Fujian 350002, PR China; Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, PR China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Key Lab of Biopesticide and Chemical Biology, Fujian Agriculture and Forestry University, Ministry of Education & Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Fuzhou, Fujian 350002, PR China.
authors.forename: Fanbing
authors.initials: F
authors.lastname: Chen
authors.affiliation: Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, PR China.
authors.forename: Yangjian
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, PR China; School of Environment and Energy, South China University of Technology, Guangzhou 510006, PR China. Electronic address: zlin@fjirsm.ac.cn.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Lin
authors.affiliation: Key Lab of Biopesticide and Chemical Biology, Fujian Agriculture and Forestry University, Ministry of Education & Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Fuzhou, Fujian 350002, PR China. Electronic address: guanxfafu@126.com.
authors.forename: Xiong
authors.initials: X
authors.lastname: Guan
mesh_terms: D000327:Adsorption; D000577:Amides / Q000737:chemistry; D001413:Bacillus thuringiensis / Q000378:metabolism*; D001673:Biodegradation, Environmental; D018533:Biomass; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008906:Mining; D010710:Phosphates / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D014501:Uranium / Q000737:chemistry*; D062065:Wastewater; D014874:Water Pollutants, Chemical / Q000032:analysis*; D014961:X-Ray Diffraction
chemical_list: D000577:Amides; D010710:Phosphates; D014501:Uranium; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Bacillus thuringiensis; Nano-uramphite; Transformation; U(VI)