title: Hydrolysis with Cucurbita ficifolia serine protease reduces antigenic response to bovine whey protein concentrate and αs-casein.
abstract: In the present study the effect of hydrolysis with non-commercial Cucurbita ficifolia serine protease on a reduction of the IgE and IgG binding capacity of whey protein concentrate and αs-casein was investigated. The intensity of the protein degradation was analyzed by the degree of hydrolysis, the free amino groups content and RP-HPLC. The ability to bind the antibodies by native proteins and their hydrolysates was determined using a competitive ELISA test. Deep hydrolysis contributed to a significant reduction of immunoreactive epitopes present in WPC. In the case of IgE and IgG present in the serum pool of children with CMA, the lowest binding capacity was detected in the 24 h WPC hydrolysate, where the inhibition of the reaction with native WPC was ≤23 and ≤60 %, respectively. The analysis of the IgG reactivity in the antiserum of the immunized goat showed that the lowest antibody binding capacity was exhibited also by 24 h WPC hydrolysate at a concentration of 1000 μg/ml where the inhibition of the reaction with nWPC was ≤47 %. One-hour hydrolysis of α-casein was sufficient to significant reduction of the protein antigenicity, while the longer time (5 h) of hydrolysis probably lead to the appearance of new epitopes reactive with polyclonal.
authors.affiliation: Department of Animal Products Technology and Quality Management, Wrocław University of Environmental and Life Sciences, ul. Chełmońskiego 37/41, 51-630, Wrocław, Poland. konrad.babij@gmail.com.
authors.forename: Konrad
authors.initials: K
authors.lastname: Babij
authors.affiliation: Department of Immunology, Pathophysiology and Veterinary Preventive Medicine, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Bajzert
authors.affiliation: Department of Animal Products Technology and Quality Management, Wrocław University of Environmental and Life Sciences, ul. Chełmońskiego 37/41, 51-630, Wrocław, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Dąbrowska
authors.affiliation: Department of Animal Products Technology and Quality Management, Wrocław University of Environmental and Life Sciences, ul. Chełmońskiego 37/41, 51-630, Wrocław, Poland.
authors.forename: Marek
authors.initials: M
authors.lastname: Szołtysik
authors.affiliation: Department of Animal Products Technology and Quality Management, Wrocław University of Environmental and Life Sciences, ul. Chełmońskiego 37/41, 51-630, Wrocław, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Zambrowicz
authors.affiliation: Department of Pediatrics, Medical University of Vienna, Währinger Gürtel 18, 1090, Vienna, Austria.
authors.forename: Gert
authors.initials: G
authors.lastname: Lubec
authors.affiliation: Department of Immunology, Pathophysiology and Veterinary Preventive Medicine, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Stefaniak
authors.affiliation: 1st Department and Clinic of Pediatric Allergology and Cardiology, Wrocław Medical University, M. Hoene-Wronskiego 13c, 50-376, Wroclaw, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Willak-Janc
authors.affiliation: Department of Animal Products Technology and Quality Management, Wrocław University of Environmental and Life Sciences, ul. Chełmońskiego 37/41, 51-630, Wrocław, Poland.
authors.forename: Józefa
authors.initials: J
authors.lastname: Chrzanowska
mesh_terms: D000818:Animals; D000941:Antigens / Q000737:chemistry*; D002364:Caseins / Q000737:chemistry*; D002417:Cattle; D028464:Cucurbita / Q000201:enzymology*; D006868:Hydrolysis; D007073:Immunoglobulin E / Q000737:chemistry; D007074:Immunoglobulin G / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry*; D057057:Serine Proteases / Q000737:chemistry*; D000067816:Whey Proteins / Q000737:chemistry*
chemical_list: D000941:Antigens; D002364:Caseins; D007074:Immunoglobulin G; D010940:Plant Proteins; D000067816:Whey Proteins; D007073:Immunoglobulin E; D057057:Serine Proteases
keywords: Cow’s milk allergy; Cucurbita ficifolia; Serine protease; Whey protein hydrolysates; αs-casein hydrolysates