title: Comparison of two low-density lipoprotein apheresis systems in patients with homozygous familial hypercholesterolemia.
abstract: Low-density lipoprotein (LDL) apheresis (LA) is a reliable method to decrease LDL-C concentrations and remains the gold standard therapy in homozygous familial hypercholesterolemia (HoFH). The objective of this study was to compare the efficacy of two LA systems [heparin-induced extracorporeal LDL precipitation (HELP) vs. dextran sulfate adsorption (DS) on the reduction of lipids, inflammatory markers, and adhesion molecules in a sample of genetically defined HoFH subjects (n = 9)]. Fasting blood samples were collected before and after LA. All subjects served as their own control and were first treated with the HELP system then with DS in this single sequence study. Compared with HELP, DS led to significantly greater reductions in total cholesterol (-63.3% vs. -59.9%; P = 0.05), LDL-C (-70.5% vs. -63.0%; P = 0.02), CRP (-75.3% vs. -48.8%; P < 0.0001), and TNF-α (-23.7% vs. +14.7%; P = 0.003). Reductions in the plasma levels of PCSK9 (-45.3% vs. -63.4%; P = 0.31), lipoprotein (a) (-70.6% vs. -65.0%; P = 0.30), E-selectin (-16.6% vs. -18.3%; P = 0.65), ICAM-1 (-4.0 vs. 5.6%; P = 0.56), and VCAM-1 (8.3% vs. -1.8%; P = 0.08) were not different between the two systems. For the same volume of filtered plasma (3,000 mL), however, HELP led to greater reductions in plasma apoB (-63.1% vs. -58.3%; P = 0.04), HDL-C (-20.6% vs. -6.5%; P = 0.003), and PCSK9 (-63.4% vs. -28.5%; P = 0.02) levels. These results suggest that both LA systems are effective in reducing plasma lipids and inflammatory markers in HoFH. Compared with HELP, greater reductions in lipid levels and inflammatory markers were achieved with DS, most likely because this method allows for a larger plasma volume to be filtered. J. Clin. Apheresis 31:359-367, 2016. © 2015 Wiley Periodicals, Inc.
authors.affiliation: Institute of Nutrition and Functional Foods, Laval University, Quebec City, Québec, Canada.
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Drouin-Chartier
authors.affiliation: Institute of Nutrition and Functional Foods, Laval University, Quebec City, Québec, Canada.
authors.forename: André J
authors.initials: AJ
authors.lastname: Tremblay
authors.affiliation: Lipid Research Centre, Centre Hospitalier Universitaire de Québec Research Centre, Québec City, Québec, Canada.
authors.forename: Jean
authors.initials: J
authors.lastname: Bergeron
authors.affiliation: Lipid Research Centre, Centre Hospitalier Universitaire de Québec Research Centre, Québec City, Québec, Canada.
authors.forename: Maude
authors.initials: M
authors.lastname: Pelletier
authors.affiliation: Lipid Research Centre, Centre Hospitalier Universitaire de Québec Research Centre, Québec City, Québec, Canada.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Laflamme
authors.affiliation: Institute of Nutrition and Functional Foods, Laval University, Quebec City, Québec, Canada.
authors.forename: Benoît
authors.initials: B
authors.lastname: Lamarche
authors.affiliation: Institute of Nutrition and Functional Foods, Laval University, Quebec City, Québec, Canada.|Lipid Research Centre, Centre Hospitalier Universitaire de Québec Research Centre, Québec City, Québec, Canada.
authors.forename: Patrick
authors.initials: P
authors.lastname: Couture
mesh_terms: D000327:Adsorption / Q000187:drug effects; D000328:Adult; D015415:Biomarkers / Q000097:blood; D001781:Blood Component Removal / Q000379:methods* / Q000592:standards; D011232:Chemical Precipitation / Q000187:drug effects; D008078:Cholesterol, LDL / Q000097:blood; D016264:Dextran Sulfate / Q000627:therapeutic use; D006493:Heparin / Q000627:therapeutic use; D006720:Homozygote; D006801:Humans; D006938:Hyperlipoproteinemia Type II / Q000097:blood / Q000628:therapy*; D007249:Inflammation / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood / Q000302:isolation & purification*; D008875:Middle Aged
chemical_list: D015415:Biomarkers; D008078:Cholesterol, LDL; D008077:Lipoproteins, LDL; D006493:Heparin; D016264:Dextran Sulfate
keywords: apheresis; familial hypercholesterolemia; inflammation; lipoproteins