title: Construction of a reusable multi-enzyme supramolecular device via disulfide bond locking.
abstract: A strategy for constructing a reusable multi-enzyme supramolecular device was developed by reprogramming protein-protein interactions and disulfide bond locking. The resultant multi-enzyme supramolecular device demonstrated good reusability, and approximately 80% of its initial catalytic activity was retained even after eight cycles of reuse.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, China. yhren@ecust.edu.cn dzhwei@ecust.edu.cn.
authors.forename: Xin
authors.initials: X
authors.lastname: Gao
authors.forename: Chengcheng
authors.initials: C
authors.lastname: Zhao
authors.forename: Ting
authors.initials: T
authors.lastname: Yu
authors.forename: Shengli
authors.initials: S
authors.lastname: Yang
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Ren
authors.forename: Dongzhi
authors.initials: D
authors.lastname: Wei
mesh_terms: D055162:Biocatalysis; D004220:Disulfides / Q000737:chemistry*; D005560:Formate Dehydrogenases / Q000737:chemistry* / Q000378:metabolism*; D050803:Leucine Dehydrogenase / Q000737:chemistry* / Q000378:metabolism*; D008024:Ligands; D008958:Models, Molecular; D054731:PDZ Domains
chemical_list: D004220:Disulfides; D008024:Ligands; D005560:Formate Dehydrogenases; D050803:Leucine Dehydrogenase