title: Succinic acid production from xylose mother liquor by recombinantEscherichia colistrain.
abstract: Succinic acid (1,4-butanedioic acid) is identified as one of important building-block chemicals. Xylose mother liquor is an abundant industrial residue in xylitol biorefining industry. In this study, xylose mother liquor was utilized to produce succinic acid by recombinantEscherichia colistrain SD121, and the response surface methodology was used to optimize the fermentation media. The optimal conditions of succinic acid fermentation were as follows: 82.62 g L-1total initial sugars, 42.27 g L-1MgCO3and 17.84 g L-1yeast extract. The maximum production of succinic acid was 52.09 ± 0.21 g L-1after 84 h with a yield of 0.63 ± 0.03 g g-1total sugar, approaching the predicted value (53.18 g L-1). It was 1.78-fold of the production of that obtained with the basic medium. This was the first report on succinic acid production from xylose mother liquor by recombinantE. colistrains with media optimization using response surface methodology. This work suggested that the xylose mother liquor could be an alternative substrate for the economical production of succinic acid by recombinantE. colistrains.
authors.affiliation: College of Chemistry and Chemical Engineering, Chongqing University , Chongqing 400044 , China.
authors.forename: Honghui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Biomedical and Chemical Engineering, Syracuse University , Syracuse, NY 13244 , USA.
authors.forename: Jiachuan
authors.initials: J
authors.lastname: Pan
authors.affiliation: College of Chemistry and Chemical Engineering, Chongqing University , Chongqing 400044 , China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Chemistry and Chemical Engineering, Chongqing University , Chongqing 400044 , China.
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.affiliation: College of Chemistry and Chemical Engineering, Chongqing University , Chongqing 400044 , China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering , Chinese Academy of Sciences , Beijing 100190 , China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: College of Chemistry and Chemical Engineering, Chongqing University , Chongqing 400044 , China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.affiliation: College of Chemistry and Chemical Engineering, Chongqing University , Chongqing 400044 , China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Zhou
keywords: Escherichia coli; fermentation; response surface methodology; succinic acid; xylose mother liquor