title: [Frontiers in Live Bone Imaging Researches. Development of fluorescent pH probe for imaging osteoclast activation].
abstract: For the clinical diagnosis of osteoporosis, X-ray CT and biochemical bone metabolism markers, however, there is no method to monitor osteoclast activity in the living system. We have developed the fluorescent probe to monitor osteoclast activity, by the combination of pH sensitive fluorescent dye with bisphosphonate which is delivered to the bone surface by its high affinity to hydroxyl apatite, which is named BAp-E. In vivo mouse imaging of activated osteoclast was successfully demonstrated using two photon excitation laser microscope.
authors.affiliation: Department of Material and Life Science, Division of Advanced Science and Biotechnology, Graduate School of Engineering, Osaka University, Japan.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Kikuchi
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Kowada
authors.forename: Hiroki
authors.initials: H
authors.lastname: Maeda
mesh_terms: D015195:Drug Design; D005456:Fluorescent Dyes; D006801:Humans; D006863:Hydrogen-Ion Concentration; D018613:Microscopy, Confocal / Q000379:methods; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods; D057054:Molecular Imaging / Q000379:methods*; D010010:Osteoclasts / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D005456:Fluorescent Dyes