title: In silico Neuropeptidome of Female Macrobrachium rosenbergii Based on Transcriptome and Peptide Mining of Eyestalk, Central Nervous System and Ovary.
abstract: Macrobrachium rosenbergii is the most economically important of the cultured freshwater crustacean species, yet there is currently a deficiency in genomic and transcriptomic information for research requirements. In this study, we present an in silico analysis of neuropeptide genes within the female M. rosenbergii eyestalk, central nervous system, and ovary. We could confidently predict 37 preproneuropeptide transcripts, including those that encode bursicons, crustacean cardioactive peptide, crustacean hyperglycemic hormones, eclosion hormone, pigment-dispersing hormones, diuretic hormones, neuropeptide F, neuroparsins, SIFamide, and sulfakinin. These transcripts are most prominent within the eyestalk and central nervous system. Transcript tissue distribution as determined by reverse transcription-polymerase chain reaction revealed the presence of selected neuropeptide genes of interest mainly in the nervous tissues while others were additionally present in the non-nervous tissues. Liquid chromatography-mass spectrometry analysis of eyestalk peptides confirmed the presence of the crustacean hyperglycemic hormone precursor. This data set provides a strong foundation for further studies into the functional roles of neuropeptides in M. rosenbergii, and will be especially helpful for developing methods to improve crustacean aquaculture.
authors.affiliation: Department of Anatomy, Faculty of Science, Mahidol University, Bangkok, Thailand.
authors.forename: Saowaros
authors.initials: S
authors.lastname: Suwansa-Ard
authors.affiliation: Department of Anatomy, Faculty of Science, Mahidol University, Bangkok, Thailand.
authors.forename: Tipsuda
authors.initials: T
authors.lastname: Thongbuakaew
authors.affiliation: Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Maroochydore, Queensland, Australia.
authors.forename: Tianfang
authors.initials: T
authors.lastname: Wang
authors.affiliation: Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Maroochydore, Queensland, Australia.
authors.forename: Min
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Maroochydore, Queensland, Australia.
authors.forename: Abigail
authors.initials: A
authors.lastname: Elizur
authors.affiliation: Department of Anatomy, Faculty of Science, Mahidol University, Bangkok, Thailand; Pro Vice-Chancellor's Office, Faculty of Science, Engineering and Built Environment, Deakin University, Geelong, Victoria, Australia.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Hanna
authors.affiliation: Department of Anatomy, Faculty of Science, Mahidol University, Bangkok, Thailand.
authors.forename: Prapee
authors.initials: P
authors.lastname: Sretarugsa
authors.affiliation: Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Maroochydore, Queensland, Australia.
authors.forename: Scott F
authors.initials: SF
authors.lastname: Cummins
authors.affiliation: Department of Anatomy, Faculty of Science, Mahidol University, Bangkok, Thailand.
authors.forename: Prasert
authors.initials: P
authors.lastname: Sobhon
mesh_terms: D000818:Animals; D005260:Female; D009420:Nervous System / Q000378:metabolism; D009479:Neuropeptides / Q000235:genetics / Q000378:metabolism*; D010053:Ovary / Q000378:metabolism; D033541:Palaemonidae / Q000235:genetics / Q000378:metabolism*; D059467:Transcriptome / Q000235:genetics
chemical_list: D009479:Neuropeptides