title: Mitochondrially targeted redox probe reveals the variations in oxidative capacity of the haematopoietic cells.
abstract: Both oxidative stress and mitochondrial dysfunction play roles in a myriad of pathological conditions. There is therefore a need for tools that possess the ability to measure the dynamics of oxidative capacity within the mitochondria, particularly those that can measure reversible changes. Here, we report a mitochondrially-targeted fluorescent redox sensor NpFR2, which can reversibly measure changes in the mitochondrial redox environment. The probe has been used to report on variations in oxidative capacity of the haematopoietic cells in bone marrow, thymus and spleen.
authors.affiliation: School of Chemistry, The University of Sydney, New South Wales 2006, Australia. elizabeth.new@sydney.edu.au.
authors.forename: Amandeep
authors.initials: A
authors.lastname: Kaur
authors.forename: Kurt W L
authors.initials: KW
authors.lastname: Brigden
authors.forename: Timothy F
authors.initials: TF
authors.lastname: Cashman
authors.forename: Stuart T
authors.initials: ST
authors.lastname: Fraser
authors.forename: Elizabeth J
authors.initials: EJ
authors.lastname: New
mesh_terms: D000818:Animals; D002460:Cell Line; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D006367:HeLa Cells; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008928:Mitochondria / Q000378:metabolism*; D053644:Naphthalimides / Q000737:chemistry* / Q000378:metabolism; D061848:Optical Imaging; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000032:analysis / Q000378:metabolism*; D013154:Spleen / Q000166:cytology; D013950:Thymus Gland / Q000166:cytology
chemical_list: D005456:Fluorescent Dyes; D053644:Naphthalimides; D017382:Reactive Oxygen Species