title: A miniaturized readout strategy for endogenous histone deacetylase activity.
abstract: Histone deacetylases are important drug targets, which are difficult to characterize due to their poor accessibility. We have developed a miniaturized assay for the multi-site readout of deacetylase activity and profiled the substrate selectivity of HDACs for acetylation sites on histone H4 and tumor suppressor protein p53.
authors.affiliation: Interfaculty Institute of Biochemistry, University of Tuebingen, Hoppe-Seyler-Str. 4, 72076 Tuebingen, Germany. dirk.schwarzer@uni-tuebingen.de.
authors.forename: Jan Oliver
authors.initials: JO
authors.lastname: Jost
authors.forename: Alfred
authors.initials: A
authors.lastname: Hanswillemenke
authors.forename: Dirk
authors.initials: D
authors.lastname: Schwarzer
mesh_terms: D000107:Acetylation; D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D057075:Enzyme Assays; D006367:HeLa Cells; D056572:Histone Deacetylase Inhibitors / Q000737:chemistry; D006655:Histone Deacetylases / Q000737:chemistry*; D006801:Humans; D006877:Hydroxamic Acids / Q000737:chemistry; D007700:Kinetics; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry; D012097:Repressor Proteins / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; D009842:Oligopeptides; D012097:Repressor Proteins; C012589:trichostatin A; C409470:HDAC8 protein, human; D006655:Histone Deacetylases