title: Mechanical Overloading Increases Maximal Force and Reduces Fragility in Hind Limb Skeletal Muscle from Mdx Mouse.
abstract: There is fear that mechanical overloading (OVL; ie, high-force contractions) accelerates Duchenne muscular dystrophy. Herein, we determined whether short-term OVL combined with wheel running, short-term OVL combined with irradiation, and long-term OVL are detrimental for hind limb mdx mouse muscle, a murine model of Duchene muscular dystrophy exhibiting milder dystrophic features. OVL was induced by the surgical ablation of the synergic muscles of the plantaris muscle, a fast muscle susceptible to contraction-induced muscle damage in mdx mice. We found that short-term OVL combined with wheel and long-term OVL did not worsen the deficit in specific maximal force (ie, absolute maximal force normalized to muscle size) and histological markers of muscle damage (percentage of regenerating fibers and fibrosis) in mdx mice. Moreover, long-term OVL did not increase the alteration in calcium homeostasis and did not deplete muscle cell progenitors expressing Pax 7 in mdx mice. Irradiation before short-term OVL, which is believed to inhibit muscle regeneration, was not more detrimental to mdx than control mice. Interestingly, short-term OVL combined with wheel and long-term OVL markedly improved the susceptibility to contraction-induced damage, increased absolute maximal force, induced hypertrophy, and promoted a slower, more oxidative phenotype. Together, these findings indicate that OVL is beneficial to mdx muscle, and muscle regeneration does not mask the potentially detrimental effect of OVL.
authors.affiliation: Myology Research Center, UM76 INSERM U974, Centre National de la recherche scientifique FRE 3617, Institut de Myologie, University of Pierre et Marie Curie-Paris 6, Sorbonne Universités, Paris, France; University of Paris Descartes, Sorbonne Paris Cité, Paris, France. Electronic address: arnaud.ferry@upmc.fr.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Ferry
authors.affiliation: Biological Adaptation and Aging, Institut de Biologie Paris-Seine, Centre National de la Recherche Scientifique UMR 8256, Université Pierre et Marie Curie-Paris 6, Sorbonne Universités, Paris, France.
authors.forename: Ara
authors.initials: A
authors.lastname: Parlakian
authors.affiliation: Biological Adaptation and Aging, Institut de Biologie Paris-Seine, Centre National de la Recherche Scientifique UMR 8256, Université Pierre et Marie Curie-Paris 6, Sorbonne Universités, Paris, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Joanne
authors.affiliation: Myology Research Center, UM76 INSERM U974, Centre National de la recherche scientifique FRE 3617, Institut de Myologie, University of Pierre et Marie Curie-Paris 6, Sorbonne Universités, Paris, France.
authors.forename: Bodvael
authors.initials: B
authors.lastname: Fraysse
authors.affiliation: Myology Research Center, UM76 INSERM U974, Centre National de la recherche scientifique FRE 3617, Institut de Myologie, University of Pierre et Marie Curie-Paris 6, Sorbonne Universités, Paris, France; Biological Adaptation and Aging, Institut de Biologie Paris-Seine, Centre National de la Recherche Scientifique UMR 8256, Université Pierre et Marie Curie-Paris 6, Sorbonne Universités, Paris, France.
authors.forename: Takouhie
authors.initials: T
authors.lastname: Mgrditchian
authors.affiliation: Myology Research Center, UM76 INSERM U974, Centre National de la recherche scientifique FRE 3617, Institut de Myologie, University of Pierre et Marie Curie-Paris 6, Sorbonne Universités, Paris, France.
authors.forename: Pauline
authors.initials: P
authors.lastname: Roy
authors.affiliation: Myology Research Center, UM76 INSERM U974, Centre National de la recherche scientifique FRE 3617, Institut de Myologie, University of Pierre et Marie Curie-Paris 6, Sorbonne Universités, Paris, France.
authors.forename: Denis
authors.initials: D
authors.lastname: Furling
authors.affiliation: Myology Research Center, UM76 INSERM U974, Centre National de la recherche scientifique FRE 3617, Institut de Myologie, University of Pierre et Marie Curie-Paris 6, Sorbonne Universités, Paris, France.
authors.forename: Gillian
authors.initials: G
authors.lastname: Butler-Browne
authors.affiliation: Biological Adaptation and Aging, Institut de Biologie Paris-Seine, Centre National de la Recherche Scientifique UMR 8256, Université Pierre et Marie Curie-Paris 6, Sorbonne Universités, Paris, France.
authors.forename: Onnik
authors.initials: O
authors.lastname: Agbulut
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D006984:Hypertrophy; D035002:Lower Extremity; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D009043:Motor Activity; D009119:Muscle Contraction; D018482:Muscle, Skeletal / Q000503:physiopathology* / Q000528:radiation effects; D009137:Muscular Dystrophy, Animal / Q000503:physiopathology*; D020388:Muscular Dystrophy, Duchenne / Q000503:physiopathology*; D009154:Mutation; D012038:Regeneration; D032496:Satellite Cells, Skeletal Muscle / Q000502:physiology / Q000528:radiation effects