title: Tyrosinase inhibitor screening in traditional Chinese medicines by electrophoretically mediated microanalysis.
abstract: A capillary-electrophoresis-based method for the screening of tyrosinase inhibitors in traditional Chinese medicines was developed. The method integrated electrophoretically mediated microanalysis with sandwich mode injection, partial filling, and rapid polarity switching techniques, and carried out on-column enzyme reaction and the separation of substrate and product. The conditions were optimized including the background electrolyte, mixing voltage, and the incubation time. Finally, the screening of nine standard natural compounds of traditional Chinese medicines was carried out. The inhibitors can be directly identified from the reduced peak area of the product compared to that obtained without any inhibitor. Chlorogenic acid (100 μM) showed inhibitory activity with the inhibitory percentage of 19.8%, while the other compounds showed no inhibitory activity. This method has great application potential in drug discovery from traditional Chinese medicines.
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and School of Pharmaceutical Sciences, Wuhan University, Wuhan, China.|State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Lilin
authors.initials: L
authors.lastname: Tang
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and School of Pharmaceutical Sciences, Wuhan University, Wuhan, China.
authors.forename: Wenpeng
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and School of Pharmaceutical Sciences, Wuhan University, Wuhan, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and School of Pharmaceutical Sciences, Wuhan University, Wuhan, China.|State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Zilin
authors.initials: Z
authors.lastname: Chen
mesh_terms: D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D004791:Enzyme Inhibitors / Q000032:analysis*; D008516:Medicine, Chinese Traditional; D014442:Monophenol Monooxygenase / Q000032:analysis / Q000037:antagonists & inhibitors*
chemical_list: D004365:Drugs, Chinese Herbal; D004791:Enzyme Inhibitors; D014442:Monophenol Monooxygenase
keywords: Electrophoretically mediated microanalysis; Inhibitor screening; Traditional Chinese medicines; Tyrosinase