title: Efficient chemoenzymatic synthesis of novel galacto-N-biose derivatives and their sialylated forms.
abstract: Galacto-N-biose (GNB) derivatives were efficiently synthesized from galactose derivatives via a one-pot two-enzyme system containing two promiscuous enzymes from Bifidobacterium infantis: a galactokinase (BiGalK) and a d-galactosyl-β1-3-N-acetyl-d-hexosamine phosphorylase (BiGalHexNAcP). Mono-sialyl and di-sialyl galacto-N-biose derivatives were then prepared using a one-pot two-enzyme system containing a CMP-sialic acid synthetase and an α2-3-sialyltransferase or an α2-6-sialyltransferase.
authors.affiliation: Department of Chemistry and Center for Diagnostics & Therapeutics, Georgia State University, Atlanta, GA 30303, USA. lli22@gsu.edu pwang11@gsu.edu.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Liu
authors.forename: Tiehai
authors.initials: T
authors.lastname: Li
authors.forename: Wenjun
authors.initials: W
authors.lastname: Wang
authors.forename: Zaikuan
authors.initials: Z
authors.lastname: Yu
authors.forename: Cheng
authors.initials: C
authors.lastname: Ma
authors.forename: Jingyao
authors.initials: J
authors.lastname: Qu
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Peng G
authors.initials: PG
authors.lastname: Wang
mesh_terms: D001644:Bifidobacterium / Q000201:enzymology; D004187:Disaccharides / Q000138:chemical synthesis*; D005685:Galactans / Q000138:chemical synthesis*; D005686:Galactokinase / Q000737:chemistry / Q000378:metabolism; D005700:Galactosyltransferases / Q000737:chemistry / Q000378:metabolism; D000216:N-Acylneuraminate Cytidylyltransferase / Q000737:chemistry / Q000378:metabolism; D012794:Sialic Acids / Q000138:chemical synthesis*; D012799:Sialyltransferases / Q000737:chemistry / Q000378:metabolism; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase; D000094663:beta-Galactoside alpha-2,3-Sialyltransferase
chemical_list: D004187:Disaccharides; D005685:Galactans; D012794:Sialic Acids; D005700:Galactosyltransferases; C118176:beta-1,3-galactosyl-N-acetylhexosamine phosphorylase; D012799:Sialyltransferases; D005686:Galactokinase; D000216:N-Acylneuraminate Cytidylyltransferase; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase; D000094663:beta-Galactoside alpha-2,3-Sialyltransferase