title: Oxidized LDL Is Strictly Limited to Hyperthyroidism Irrespective of Fat Feeding in Female Sprague Dawley Rats.
abstract: Metabolic dysfunctions might play a crucial role in the pathophysiology of thyroid dysfunctions. This study aimed to investigate the impact of a controlled diet (normal versus high fat feeding) on hypothyroid and hyperthyroid Sprague Dawley rats. Female Sprague Dawley rats (n = 66) were grouped into normal diet (n = 30) and high-fat diet (n = 36) groups and subdivided into controls, hypothyroid and hyperthyroid groups, induced through propylthiouracil or triiodothyronine (T3) treatment, respectively. After 12 weeks of treatment metabolic parameters, such as oxidized LDL (oxLDL), malondialdehyde (MDA), 4-hydroxynonenal (HNE), the lipid profile, body weight and food intake parameters were analyzed. Successfully induced thyroid dysfunctions were shown by T3 levels, both under normal and high fat diet. Thyroid dysfunctions were accompanied by changes in calorie intake and body weight as well as in the lipid profile. In detail, hypothyroid rats showed significantly decreased oxLDL levels, whereas hyperthyroid rats showed significantly increased oxLDL levels. These effects were seen under high fat diet and were less pronounced with normal feeding. Taken together, we showed for the first time in female SD rats that only hyper-, but not hypothyroidism, is associated with high atherogenic oxidized LDL irrespective of normal or high-fat diet in Sprague Dawley rats.
authors.affiliation: Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University Graz, 8036 Graz, Austria. sieglinde.zelzer@klinikum-graz.at.
authors.forename: Sieglinde
authors.initials: S
authors.lastname: Zelzer
authors.affiliation: Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University Graz, 8036 Graz, Austria. harald.mangge@klinikum-graz.at.
authors.forename: Harald
authors.initials: H
authors.lastname: Mangge
authors.affiliation: Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University Graz, 8036 Graz, Austria. sabine.pailer@klinikum-graz.at.
authors.forename: Sabine
authors.initials: S
authors.lastname: Pailer
authors.affiliation: Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University Graz, 8036 Graz, Austria. herwig.ainoedhofer@klinikum-graz.at.
authors.forename: Herwig
authors.initials: H
authors.lastname: Ainoedhofer
authors.affiliation: Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University Graz, 8036 Graz, Austria. petra.kieslinger@klinikum-graz.at.
authors.forename: Petra
authors.initials: P
authors.lastname: Kieslinger
authors.affiliation: Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University Graz, 8036 Graz, Austria. tatjana.stojakovic@medunigraz.at.
authors.forename: Tatjana
authors.initials: T
authors.lastname: Stojakovic
authors.affiliation: Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University Graz, 8036 Graz, Austria. hubert.scharnagl@medunigraz.at.
authors.forename: Hubert
authors.initials: H
authors.lastname: Scharnagl
authors.affiliation: Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University Graz, 8036 Graz, Austria. florian.prueller@klinikum-graz.at.
authors.forename: Florian
authors.initials: F
authors.lastname: Prüller
authors.affiliation: Department of Pediatrics, Paracelsus Medical University Salzburg, 5020 Salzburg, Austria. c.datz@kh-oberndorf.at.|Obesity Research Unit, Paracelsus Medical University and Salzburger Landeskliniken, 5020 Salzburg, Austria. c.datz@kh-oberndorf.at.
authors.forename: Daniel
authors.initials: D
authors.lastname: Weghuber
authors.affiliation: Obesity Research Unit, Paracelsus Medical University and Salzburger Landeskliniken, 5020 Salzburg, Austria. c.datz@kh-oberndorf.at.|Department of Internal Medicine, General Hospital Oberndorf, 5110 Oberndorf, Austria. c.datz@kh-oberndorf.at.
authors.forename: Christian
authors.initials: C
authors.lastname: Datz
authors.affiliation: Department of Pathology, Medical University of Graz, 8036 Graz, Austria. johannes.haybaeck@medunigraz.at.
authors.forename: Johannes
authors.initials: J
authors.lastname: Haybaeck
authors.affiliation: Division of Endocrinology and Metabolism, Medical University Graz, 8036 Graz, Austria. barbara.obermayer@medunigraz.at.
authors.forename: Barbara
authors.initials: B
authors.lastname: Obermayer-Pietsch
authors.affiliation: Division of Endocrinology and Metabolism, Medical University Graz, 8036 Graz, Austria. christian.trummer@klinikum-graz.at.
authors.forename: Christian
authors.initials: C
authors.lastname: Trummer
authors.affiliation: Medical Biochemistry, Center of Chemistry and Biomedicine, Medical University of Innsbruck, 6020 Innsbruck, Austria. johanna.gostner@i-med.ac.at.
authors.forename: Johanna
authors.initials: J
authors.lastname: Gostner
authors.affiliation: Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University Graz, 8036 Graz, Austria. hans.gruber@medunigraz.at.
authors.forename: Hans-Jürgen
authors.initials: HJ
authors.lastname: Gruber
mesh_terms: D000447:Aldehydes / Q000097:blood / Q000378:metabolism; D000818:Animals; D001835:Body Weight; D059305:Diet, High-Fat; D005260:Female; D006980:Hyperthyroidism / Q000097:blood / Q000378:metabolism* / Q000503:physiopathology; D007037:Hypothyroidism / Q000097:blood / Q000378:metabolism / Q000503:physiopathology; D050356:Lipid Metabolism; D008055:Lipids / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood / Q000378:metabolism*; D008315:Malondialdehyde / Q000097:blood / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013961:Thyroid Gland / Q000378:metabolism / Q000503:physiopathology*
chemical_list: D000447:Aldehydes; D008055:Lipids; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; D008315:Malondialdehyde; C027576:4-hydroxy-2-nonenal
keywords: Sprague Dawley rats; fat feeding; hyperthyroidism; hypothyroidism; metabolic dysfunction; oxidized LDL