title: The lectin fromMusa paradisiacabinds with the capsid protein of tobacco mosaic virus and prevents viral infection.
abstract: It has been demonstrated that the lectin fromMusa paradisiaca(BanLec-1) could inhibit the cellular entry of human immunodeficiency virus (HIV). In order to evaluate its effects on tobacco mosaic virus (TMV), thebanlec-1gene was cloned and transformed intoEscherichia coliand tobacco, respectively. Recombinant BanLec-1 showed metal ions dependence, and higher thermal and pH stability. Overexpression ofbanlec-1in tobacco resulted in decreased leaf size, and higher resistance to TMV infection, which includes reduced TMV cellular entry, more stable chlorophyll contents, and enhanced antioxidant enzymes. BanLec-1 was found to bind directly to the TMV capsid proteinin vitro, and to inhibit TMV infection in a dose-dependent manner. In contrast to limited preventionin vivo, purified rBanLec-1 exhibited more significant effects on TMV infectionin vitro. Taken together, our study indicated that BanLec-1 could prevent TMV infection in tobacco, probably through the interaction between BanLec-1 and TMV capsid protein.
authors.affiliation: Department of Biochemistry & Molecular Biology, College of Life Science, Nanjing Agricultural University , Nanjing , Jiangsu , P. R. China.
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Liu
authors.affiliation: Department of Biochemistry & Molecular Biology, College of Life Science, Nanjing Agricultural University , Nanjing , Jiangsu , P. R. China.
authors.forename: Huan
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Biochemistry & Molecular Biology, College of Life Science, Nanjing Agricultural University , Nanjing , Jiangsu , P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
keywords: BanLec-1; Musa paradisiaca; Nicotiana tabacum; capsid protein; tobacco mosaic virus; viral infection