title: Hypoglycemic, antilipidemic and antioxidant effects of valproic acid in alloxan-induced diabetic rats.
abstract: This study was designed to investigate the hypoglycemic, antilipidemic and antioxidant effects of valproic acid (VA) in alloxan-induced diabetic rats. VA (100, 300 and 600mg/kg p.o.) and insulin (17IU/kg s.c.) were administered once daily for 21 days. Fasting blood glucose level was determined at 7 days interval. On day 21, blood samples were collected for assay of serum biochemical parameters (total protein, creatinine, urea, alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), triglycerides (TG), total cholesterol (TC), high density lipoprotein (HDL), and low density lipoprotein (LDL)). Kidneys and livers were harvested for antioxidant indices and histopathological examination. In diabetic rats, VA produced a dose and day-dependent reduction in glucose level. Peak effect (52.79% reduction; P<0.001) was produced at the dose of 600mg/kg on day 21. In normoglycemic rats, VA (600mg/kg) caused significant reduction (P<0.05) in blood glucose level on days 1 and 21 with 16.38% and 15.63% reductions respectively. In diabetic rats, VA significantly reduced the level of catalase (CAT) and malondialdehyde (MDA) in the kidney, and increased the level of superoxide dismutase, CAT and glutathione peroxidase with reduction in MDA in the liver compared to diabetic control, especially at the dose of 600mg/kg. VA (600mg/kg) generally increased the level of HDL and reduced the levels of TG, LDL, TC, AST, ALT, ALP, bilirubin, creatinine and urea compared with diabetic control. The findings in this study suggest that VA possess beneficial antidiabetic effects.
authors.affiliation: Department of Pharmacology, Therapeutics and Toxicology (PTT), Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, Idi-Araba Campus, P.M.B. 12003 Lagos, Nigeria. Electronic address: ajakindele@cmul.edu.ng.
authors.forename: Abidemi J
authors.initials: AJ
authors.lastname: Akindele
authors.affiliation: Department of Pharmacology, Therapeutics and Toxicology (PTT), Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, Idi-Araba Campus, P.M.B. 12003 Lagos, Nigeria.
authors.forename: Edafe
authors.initials: E
authors.lastname: Otuguor
authors.affiliation: Department of Pharmacology, Shakambhari Institute of Higher Education and Technology, Roorkee, Uttarakhand, India.
authors.forename: Dhirendra
authors.initials: D
authors.lastname: Singh
authors.affiliation: Department of Physiology, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, Idi-Araba Campus, P.M.B. 12003 Lagos, Nigeria.
authors.forename: Duncan
authors.initials: D
authors.lastname: Ota
authors.affiliation: Department of Pathology and Forensic Medicine, Faculty of Basic Medical Sciences, Lagos State University College of Medicine, P.M.B. 21266 Ikeja, Lagos, Nigeria.
authors.forename: Adokiye S
authors.initials: AS
authors.lastname: Benebo
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D004326:Drinking / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D000960:Hypolipidemic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D050417:Insulin-Secreting Cells / Q000187:drug effects / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008055:Lipids / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D014635:Valproic Acid / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D007004:Hypoglycemic Agents; D000960:Hypolipidemic Agents; D008055:Lipids; D008315:Malondialdehyde; D014635:Valproic Acid; D006861:Hydrogen Peroxide; D002118:Calcium
keywords: Alloxan; Antilipidemic; Antioxidant; Diabetes; Hypoglycemic; Valproic acid