title: Production of L-allose and D-talose from L-psicose and D-tagatose by L-ribose isomerase.
abstract: L-ribose isomerase (L-RI) from Cellulomonas parahominis MB426 can convert L-psicose and D-tagatose to L-allose and D-talose, respectively. Partially purified recombinant L-RI from Escherichia coli JM109 was immobilized on DIAION HPA25L resin and then utilized to produce L-allose and D-talose. Conversion reaction was performed with the reaction mixture containing 10% L-psicose or D-tagatose and immobilized L-RI at 40 °C. At equilibrium state, the yield of L-allose and D-talose was 35.0% and 13.0%, respectively. Immobilized enzyme could convert L-psicose to L-allose without remarkable decrease in the enzyme activity over 7 times use and D-tagatose to D-talose over 37 times use. After separation and concentration, the mixture solution of L-allose and D-talose was concentrated up to 70% and crystallized by keeping at 4 °C. L-Allose and d-talose crystals were collected from the syrup by filtration. The final yield was 23.0% L-allose and 7.30% D-talose that were obtained from L-psicose and D-tagatose, respectively.
authors.affiliation: a The United Graduate School of Agricultural Sciences , Ehime University , Matsuyama , Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Terami
authors.forename: Keiko
authors.initials: K
authors.lastname: Uechi
authors.forename: Saki
authors.initials: S
authors.lastname: Nomura
authors.forename: Naoki
authors.initials: N
authors.lastname: Okamoto
authors.forename: Kenji
authors.initials: K
authors.lastname: Morimoto
authors.forename: Goro
authors.initials: G
authors.lastname: Takata
mesh_terms: D019747:Aldose-Ketose Isomerases / Q000737:chemistry* / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D040143:Cellulomonas / Q000737:chemistry* / Q000201:enzymology; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005632:Fructose / Q000737:chemistry / Q000378:metabolism*; D015870:Gene Expression; D005947:Glucose / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D006601:Hexoses / Q000737:chemistry / Q000378:metabolism*; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D007783:Lactones / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012266:Ribose / Q000737:chemistry / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D006601:Hexoses; D055767:Immobilized Proteins; C510913:L-talo-gamma-lactone; D007783:Lactones; D011994:Recombinant Proteins; C003243:psicose; D005632:Fructose; C002055:allose; D012266:Ribose; D019747:Aldose-Ketose Isomerases; C022546:ribose isomerase; D005947:Glucose; C030192:tagatose
keywords: d-talose; l-allose; l-ribose isomerase; rare sugar