title: Component analyses of urinary nanocrystallites of uric acid stone formers by combination of high-resolution transmission electron microscopy, fast Fourier transformation, energy dispersive X-ray spectroscopy, X-ray diffraction and Fourier transform infrared spectroscopy.
abstract: This study aimed to analyse the components of nanocrystallites in urines of patients with uric acid (UA) stones. X-ray diffraction (XRD), Fourier transform infrared spectroscopy, high-resolution transmission electron microscopy (HRTEM), fast Fourier transformation (FFT) of HRTEM, and energy dispersive X-ray spectroscopy (EDS) were performed to analyse the components of these nanocrystallites. XRD and FFT showed that the main component of urinary nanocrystallites was UA, which contains a small amount of calcium oxalate monohydrate and phosphates. EDS showed the characteristic absorption peaks of C, O, Ca and P. The formation of UA stones was closely related to a large number of UA nanocrystallites in urine. A combination of HRTEM, FFT, EDS and XRD analyses could be performed accurately to analyse the components of urinary nanocrystallites.
authors.affiliation: Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, People's Republic of China.
authors.forename: Xin-Yuan
authors.initials: XY
authors.lastname: Sun
authors.affiliation: Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, People's Republic of China.
authors.forename: Jun-Fa
authors.initials: JF
authors.lastname: Xue
authors.affiliation: Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, People's Republic of China.
authors.forename: Zhi-Yue
authors.initials: ZY
authors.lastname: Xia
authors.affiliation: Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, People's Republic of China. toyjm@jnu.edu.cn.
authors.forename: Jian-Ming
authors.initials: JM
authors.lastname: Ouyang
mesh_terms: D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013057:Spectrum Analysis; D014527:Uric Acid / Q000737:chemistry*; D014545:Urinary Calculi / Q000737:chemistry*
chemical_list: D014527:Uric Acid