title: PCR with sequence-specific primers for typing of diallelic blood groups.
abstract: PCR with sequence-specific primers (PCR-SSP) is a cost-effective and robust method for the analysis of single nucleotide polymorphisms (SNPs). Many blood group antigens and the antithetic antigens are based on a diallelic SNP in the coding region of the corresponding blood group gene. Here, we describe PCR-SSP protocols for genotyping 24 blood group antigens based on 12 diallelic SNPs. We also provide protocols for molecular determination of the rare blood group phenotypes Yk(a-) and Vel-.
authors.affiliation: Institute of Transfusion Medicine and Immunology, Medical Faculty Mannheim, German Red Cross Blood Service Baden-Württemberg - Hessen gGmbH, Heidelberg University, Friedrich-Ebert-Straße 107, 68167, Mannheim, Germany, gabi.rink@medma.uni-heidelberg.de.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Rink
authors.forename: Erwin A
authors.initials: EA
authors.lastname: Scharberg
authors.forename: Peter
authors.initials: P
authors.lastname: Bugert
mesh_terms: D000483:Alleles; D001483:Base Sequence; D001789:Blood Group Antigens / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005838:Genotype; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D020641:Polymorphism, Single Nucleotide
chemical_list: D001789:Blood Group Antigens; D017931:DNA Primers