title: Immunostaining Using an Antibody against Active Caspase-3 to Detect Apoptotic Cells in Drosophila.
abstract: The activation of mammalian caspase-3 after proteolytic cleavage adjacent to residue Asp175 produces a large (17/19-kDa), active subunit. A commercially available antibody recognizes the large, active subunit of caspase-3 but not the full-length inactive caspase-3. This antibody has also been shown to detect active Drosophila effector caspases. Here, we present a protocol showing how this antibody can be used to detect apoptotic cells in various Drosophila tissues and developmental stages and discuss the specificity of the antibody.
authors.affiliation: Centre for Cancer Biology, University of South Australia, Adelaide, South Australia 5001, Australia.
authors.forename: Donna
authors.initials: D
authors.lastname: Denton
authors.affiliation: Centre for Cancer Biology, University of South Australia, Adelaide, South Australia 5001, Australia.
authors.forename: Sharad
authors.initials: S
authors.lastname: Kumar
mesh_terms: D000818:Animals; D000906:Antibodies / Q000502:physiology*; D017209:Apoptosis / Q000502:physiology*; D053148:Caspase 3 / Q000276:immunology* / Q000378:metabolism*; D004330:Drosophila / Q000254:growth & development; D007150:Immunohistochemistry; D007814:Larva / Q000502:physiology
chemical_list: D000906:Antibodies; D053148:Caspase 3