title: A sensitive and efficient method for trace analysis of some phenolic compounds using simultaneous derivatization and air-assisted liquid-liquid microextraction from human urine and plasma samples followed by gas chromatography-nitrogen phosphorous detection.
abstract: In present study, a simultaneous derivatization and air-assisted liquid-liquid microextraction method combined with gas chromatography-nitrogen phosphorous detection has been developed for the determination of some phenolic compounds in biological samples. The analytes are derivatized and extracted simultaneously by a fast reaction with 1-flouro-2,4-dinitrobenzene under mild conditions. Under optimal conditions low limits of detection in the range of 0.05-0.34 ng mL(-1) are achievable. The obtained extraction recoveries are between 84 and 97% and the relative standard deviations are less than 7.2% for intraday (n = 6) and interday (n = 4) precisions. The proposed method was demonstrated to be a simple and efficient method for the analysis of phenols in biological samples.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.
authors.forename: Mir Ali
authors.initials: MA
authors.lastname: Farajzadeh
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Afshar Mogaddam
authors.affiliation: Food and Drug Laboratories, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Alizadeh Nabil
mesh_terms: D002849:Chromatography, Gas / Q000379:methods*; D004139:Dinitrofluorobenzene; D006801:Humans; D006863:Hydrogen-Ion Concentration; D059627:Liquid Phase Microextraction / Q000379:methods*; D009584:Nitrogen; D009994:Osmolar Concentration; D010636:Phenols / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification / Q000652:urine*; D010758:Phosphorus; D013696:Temperature
chemical_list: D010636:Phenols; D010758:Phosphorus; D004139:Dinitrofluorobenzene; D009584:Nitrogen
keywords: 1-flouro-2,4-dinitrobenzene; air-assisted liquid-liquid microextraction; gas chromatography; nitrogen-phosphorous detector; phenolic compounds