title: The identification of proteomic markers of sperm freezing resilience in ram seminal plasma.
abstract: The source and composition of seminal plasma has previously been shown to alter the ability of spermatozoa to survive cryopreservation. In the present study, the ionic and proteomic composition of seminal plasma from rams with high (HSP; n = 3) or low (LSP; n = 3) freezing resilient spermatozoa was assessed. 75 proteins were identified to be more abundant in HSP and 48 proteins were identified to be more abundant in LSP. Individual seminal plasma proteomes were established for each of the six rams examined. For each ram, correlations were conducted between previously recorded freezing resilience [1] and individual spectral counts in order to identify markers of freezing resilience. 26S proteasome complex, acylamino acid releasing enzyme, alpha mannosidase class 2C, heat shock protein 90, tripeptidyl-peptidase 2, TCP-1 complex, sorbitol dehydrogenase and transitional endoplasmic reticulum ATPase were found to be positively correlated (r(2) > 0.7) with freezing resilience. Cystatin, zinc-2-alpha glycoprotein, angiogenin-2-like protein, cartilage acidic protein-1, cathepsin B and ribonuclease 4 isoform 1 were found to be negatively correlated (r(2) > 0.7) with freezing resilience. Several negative markers were found to originate from the accessory sex glands, whereas many positive markers originated from spermatozoa and were part of or associated with the 26S proteasome or CCT complex.
authors.affiliation: Faculty of Veterinary Science, The University of Sydney, NSW 2006, Australia. Electronic address: jessica.rickard@sydney.edu.au.
authors.forename: J P
authors.initials: JP
authors.lastname: Rickard
authors.affiliation: Faculty of Veterinary Science, The University of Sydney, NSW 2006, Australia.
authors.forename: T
authors.initials: T
authors.lastname: Leahy
authors.affiliation: UMR 6175 INRA, CNRS-Université de Tours-Haras Nationaux, Station de Physiologie de la Reproduction et des Comportements, Institut National de la Recherche Agronomique, 37380 Nouzilly, France.
authors.forename: C
authors.initials: C
authors.lastname: Soleilhavoup
authors.affiliation: UMR 6175 INRA, CNRS-Université de Tours-Haras Nationaux, Station de Physiologie de la Reproduction et des Comportements, Institut National de la Recherche Agronomique, 37380 Nouzilly, France.
authors.forename: G
authors.initials: G
authors.lastname: Tsikis
authors.affiliation: UMR 6175 INRA, CNRS-Université de Tours-Haras Nationaux, Station de Physiologie de la Reproduction et des Comportements, Institut National de la Recherche Agronomique, 37380 Nouzilly, France; INRA, Plate-forme d'Analyse Intégrative des Biomolécules, Phénomique des Animaux d'Intérêt Bio-agronomique, Laboratoire de Spectrométrie de Masse, F-37380 Nouzilly, France.
authors.forename: V
authors.initials: V
authors.lastname: Labas
authors.affiliation: UMR 6175 INRA, CNRS-Université de Tours-Haras Nationaux, Station de Physiologie de la Reproduction et des Comportements, Institut National de la Recherche Agronomique, 37380 Nouzilly, France; INRA, Plate-forme d'Analyse Intégrative des Biomolécules, Phénomique des Animaux d'Intérêt Bio-agronomique, Laboratoire de Spectrométrie de Masse, F-37380 Nouzilly, France.
authors.forename: G
authors.initials: G
authors.lastname: Harichaux
authors.affiliation: Faculty of Veterinary Science, The University of Sydney, NSW 2006, Australia; Faculty of Medicine and Dentistry, The University of Sydney, NSW 2006, Australia.
authors.forename: G W
authors.initials: GW
authors.lastname: Lynch
authors.affiliation: UMR 6175 INRA, CNRS-Université de Tours-Haras Nationaux, Station de Physiologie de la Reproduction et des Comportements, Institut National de la Recherche Agronomique, 37380 Nouzilly, France.
authors.forename: X
authors.initials: X
authors.lastname: Druart
authors.affiliation: Faculty of Veterinary Science, The University of Sydney, NSW 2006, Australia.
authors.forename: S P
authors.initials: SP
authors.lastname: de Graaf
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D015925:Cryopreservation; D008297:Male; D040901:Proteomics; D012661:Semen / Q000378:metabolism*; D029589:Seminal Plasma Proteins / Q000378:metabolism*; D012756:Sheep; D013094:Spermatozoa / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D029589:Seminal Plasma Proteins
keywords: 26S proteosome; CCT complex; Cryopreservation; Proteome; Seminal plasma; Spermatozoa