title: Gold nanoparticle-based colorimetric aptasensor for rapid detection of six organophosphorous pesticides.
abstract: Fast immunoassay-based screening methods are unavailable for most small-molecule pesticides because of a lack of immunogenicity and the difficulty in obtaining antibodies by animal immunization. Aptamers are single-stranded DNA molecules selected through an in vitro process, which can bind to any target including nonimmunogenic small molecules with high affinity and specificity. Although various aptamer-based sensing methods have been developed for antibiotics, microorganisms, heavy metal ions, and biotoxins, there are few reports on aptamer-based methods for quick detection of organophosphorous pesticides. The gold (Au) nanoparticle (AuNP) colorimetric assay is a widely utilized rapid detection method because of properties such as easy operation and visualized results. In the present study, organophosphorous pesticide aptamers were adsorbed on the surface of AuNPs to stabilize the AuNP solution against high concentrations of salt to prevent AuNP aggregation. After the addition of targets, the aptamers binding to the targets are detached from the AuNPs, resulting in aggregation of AuNPs and a color change from red to purple-blue. The proposed method can detect 6 organophosphorous pesticides with good recoveries from 72% to 135% in environmental river water samples. The present study provides a new way for simple, rapid, and multiplex detection of organophosphorous pesticides.
authors.affiliation: Key Laboratory of Agro-Product Quality and Safety, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, China.|Key Laboratory of Agri-Food Quality and Safety, Ministry of Agriculture, Beijing, China.
authors.forename: Wenhui
authors.initials: W
authors.lastname: Bai
authors.affiliation: Key Laboratory of Agro-Product Quality and Safety, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, China.|Key Laboratory of Agri-Food Quality and Safety, Ministry of Agriculture, Beijing, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Agro-Product Quality and Safety, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, China.|Key Laboratory of Agri-Food Quality and Safety, Ministry of Agriculture, Beijing, China.
authors.forename: Jinchuan
authors.initials: J
authors.lastname: Liu
authors.affiliation: Key Laboratory of Agro-Product Quality and Safety, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, China.|Key Laboratory of Agri-Food Quality and Safety, Ministry of Agriculture, Beijing, China.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Yan
authors.affiliation: Key Laboratory of Agro-Product Quality and Safety, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, China.|Key Laboratory of Agri-Food Quality and Safety, Ministry of Agriculture, Beijing, China.
authors.forename: Shuming
authors.initials: S
authors.lastname: Yang
authors.affiliation: Key Laboratory of Agro-Product Quality and Safety, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, China.|Key Laboratory of Agri-Food Quality and Safety, Ministry of Agriculture, Beijing, China.
authors.forename: Ailiang
authors.initials: A
authors.lastname: Chen
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D002942:Circular Dichroism; D003124:Colorimetry; D005618:Fresh Water / Q000032:analysis; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000032:analysis*; D010575:Pesticides / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000032:analysis
chemical_list: D052157:Aptamers, Nucleotide; D009943:Organophosphorus Compounds; D010575:Pesticides; D014874:Water Pollutants, Chemical; D006046:Gold
keywords: Aptamer; Colorimetric assay; Gold nanoparticle; Organophosphorous pesticide; Rapid detection