title: Protective effects of ethyl pyruvate in cisplatin-induced nephrotoxicity.
abstract: This study was performed to investigate the effect of ethyl pyruvate on changes in renal functions and oxidative stress related renal injury caused by cisplatin (cis-dichlorodiammine platinum-II; CDDP). Male Wistar albino rats were divided into four groups (n= 8): (1) control group (1 ml Ringer's lactate solution i.p.); (2) ethyl pyruvate (EP) group (50 mg/kg Ringer's EP solution (REPS) i.p.); (3) cisplatin group (a single dose of cisplatin (5 mg/kg, i.p.); and (4) cisplatin + EP group (a single dose of cisplatin (5 mg/kg, i.p.) + REPS 50 mg/kg/day, i.p.) for five days. At the sixth day, kidneys of rats were mounted to a Langendorff apparatus. Renal perfusion pressures were recorded. Blood samples were taken for serum urea, creatinine, total oxidant status (TOS), total antioxidant status (TAS) and oxidative stres index (OSI) evaluations. Kidney tissues were obtained for malondialdehyde (MDA) analyses and histopathological examination. Perfusion pressures, serum urea, creatinine, TOS, OSI and tissue MDA levels were found significantly higher, whereas TAS was notably lower in cisplatin group. Histopathological examination showed apparent renal paranchymal injury in cisplatin group. In cisplatin + REPS group, perfusion pressures, serum urea, creatinine and tissue MDA levels were decreased. Moreover, EP co-administration provided less inflammatory cell infiltration, tubular dilatation, whereas TOS, TAS and OSI improved significantly versus cisplatin group. These findings show that EP has protective effects against cisplatin nephrotoxicity.
authors.affiliation: Department of Pharmacology, School of Medicine, Dicle University , Diyarbakir , Turkey.
authors.forename: Ilker
authors.initials: I
authors.lastname: Kelle
authors.affiliation: Department of Pharmacology, School of Medicine, Dicle University , Diyarbakir , Turkey.
authors.forename: Hasan
authors.initials: H
authors.lastname: Akkoc
authors.affiliation: Department of Histology and Embryology, School of Medicine, Dicle University , Diyarbakir , Turkey.
authors.forename: Selcuk
authors.initials: S
authors.lastname: Tunik
authors.affiliation: Department of Histology and Embryology, School of Medicine, Dicle University , Diyarbakir , Turkey.
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Nergiz
authors.affiliation: Department of Pharmacology, School of Medicine, Dicle University , Diyarbakir , Turkey.
authors.forename: Meral
authors.initials: M
authors.lastname: Erdinc
authors.affiliation: Department of Biochemistry, School of Medicine, Dicle University , Diyarbakir , Turkey.
authors.forename: Levent
authors.initials: L
authors.lastname: Erdinc
keywords: cisplatin; ethyl pyruvate; nephrotoxicity; oxidative stress index (OSI); rat; total antioxidant status (TAS); total oxidant status (TOS)