title: [Mechanism of manganese binding to leaf cell wall of Phytolacca americana L].
abstract: Phytolacca americana L. (P. americana) is a manganese (Mn) hyperaccumulator and cell wall plays an important role in the accumulation and detoxicity of Mn. We studied the impact of pH and Mn initial concentration on the binding of Mn by the leaf cell wall of P. americana, and explored the binding mechanisms by Fourier Transform Infrared Spectroscopy (FTIR) and synchrotron-based X-ray Absorption Fine Structure (XAFS) Spectroscopy. The results show that the optimum pH of Mn bingding for the leaf cell wall is between 5 and 6. The adsorption behavior of leaf cell wall can be described by Langmuir equation (R2 = 0.978 5) and the maximum adsorption of Mn on the leaf cell wall is 62.50 μmol x g(-1). Hydronyl and carbonyl groups are involved in the binding of Mn on the leaf cell wall. The Mn absorbed on the leaf cell wall is bonded by 6.3 oxygen around, and the bond length of Mn-O is 0.216 nm, which indicates the binding mechasnism of Mn to cell wall was inner-sphere complexation.
authors.forename: Xiang-Hua
authors.initials: XH
authors.lastname: Xu
authors.forename: Cui-Ying
authors.initials: CY
authors.lastname: Liu
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Man
authors.initials: M
authors.lastname: Lang
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Zhao
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Yang
authors.forename: Min
authors.initials: M
authors.lastname: Gong
mesh_terms: D001673:Biodegradation, Environmental; D002473:Cell Wall / Q000737:chemistry*; D008345:Manganese / Q000737:chemistry*; D029604:Phytolacca americana / Q000166:cytology*; D018515:Plant Leaves / Q000166:cytology*; D012989:Soil Pollutants / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D056928:X-Ray Absorption Spectroscopy
chemical_list: D012989:Soil Pollutants; D008345:Manganese