title: The novel secretory protein CGREF1 inhibits the activation of AP-1 transcriptional activity and cell proliferation.
abstract: The transcription factor AP-1 plays an important role in inflammation and cell survival. Using a dual-luciferase reporter assay system and a library of 940 candidate human secretory protein cDNA clones, we identified that CGREF1 can inhibit the transcriptional activity of AP-1. We demonstrated that CGREF1 is secreted via the classical secretory pathway through the ER-to-Golgi apparatus. Functional investigations revealed that overexpression of CGREF1 can significantly inhibit the phosphorylation of ERK and p38 MAPK, and suppress the proliferation of HEK293T and HCT116 cells. Conversely, specific siRNAs against CGREF1 can increase the transcriptional activity of AP-1. These results clearly indicated that CGREF1 is a novel secretory protein, and plays an important role in regulation of AP-1 transcriptional activity and cell proliferation.
authors.affiliation: Chinese National Human Genome Center, Beijing 100176, China; Laboratory of Medical Immunology, School of Basic Medical Science, Peking University Health Science Center, Beijing 100191, China; Peking University Center for Human Disease Genomics, Beijing 100191, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Deng
authors.affiliation: Department of Biopharmaceuticals, National Institutes of Food and Drug Control, Beijing 100050, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Wang
authors.affiliation: Peking University Center for Human Disease Genomics, Beijing 100191, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: Peking University Center for Human Disease Genomics, Beijing 100191, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Chinese National Human Genome Center, Beijing 100176, China; Laboratory of Medical Immunology, School of Basic Medical Science, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Chinese National Human Genome Center, Beijing 100176, China; Laboratory of Medical Immunology, School of Basic Medical Science, Peking University Health Science Center, Beijing 100191, China; Peking University Center for Human Disease Genomics, Beijing 100191, China. Electronic address: tpshi@126.com.
authors.forename: Taiping
authors.initials: T
authors.lastname: Shi
authors.affiliation: Chinese National Human Genome Center, Beijing 100176, China; Laboratory of Medical Immunology, School of Basic Medical Science, Peking University Health Science Center, Beijing 100191, China; Peking University Center for Human Disease Genomics, Beijing 100191, China.
authors.forename: Dalong
authors.initials: D
authors.lastname: Ma
mesh_terms: D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D049109:Cell Proliferation / Q000502:physiology; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism; D010766:Phosphorylation; D015398:Signal Transduction; D018808:Transcription Factor AP-1 / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D014162:Transfection
chemical_list: C103094:CGREF1 protein, human; D002135:Calcium-Binding Proteins; D018808:Transcription Factor AP-1; D020929:Mitogen-Activated Protein Kinase Kinases
keywords: AP-1; CGREF1; Cell proliferation; MAPK; Secretory protein