title: 454-Sequencing™ for the KEL, JR, and LAN Blood Groups.
abstract: The KEL (ISBT 006), JR (ISBT 032), and LAN (ISBT 033) blood group systems are defined by a complex genetics with a large number of exons, and numerous gene variants. In order to sequence the 53 coding exons and flanking intron regions of all three blood group genes we developed a next-generation-sequencing method using 454-sequencing™ on a Genome Sequencer (GS) Junior™ system. Multiplex analysis of eight individual DNA samples was achieved using molecular identifiers.
authors.affiliation: Institute of Transfusion Medicine and Immunology, Medical Faculty Mannheim, Heidelberg University, German Red Cross Blood Service Baden-Württemberg - Hessen, Friedrich-Ebert-Straße 107, Mannheim, 68167, Germany, wieckhusen@stud.uni-heidelberg.de.
authors.forename: Carola
authors.initials: C
authors.lastname: Wieckhusen
authors.forename: Peter
authors.initials: P
authors.lastname: Bugert
mesh_terms: D001789:Blood Group Antigens / Q000235:genetics*; D060005:Genotyping Techniques / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D007626:Kell Blood-Group System / Q000235:genetics; D009691:Nucleic Acid Denaturation; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods*; D054928:Validation Studies as Topic
chemical_list: D001789:Blood Group Antigens; D007626:Kell Blood-Group System