title: Radiosynthesis of 6'-Deoxy-6'[18F]Fluorosucrose via Automated Synthesis and Its Utility to Study In Vivo Sucrose Transport in Maize (Zea mays) Leaves.
abstract: Sugars produced from photosynthesis in leaves are transported through the phloem tissues within veins and delivered to non-photosynthetic organs, such as roots, stems, flowers, and seeds, to support their growth and/or storage of carbohydrates. However, because the phloem is located internally within the veins, it is difficult to access and to study the dynamics of sugar transport. Radioactive tracers have been extensively used to study vascular transport in plants and have provided great insights into transport dynamics. To better study sucrose partitioning in vivo, a novel radioactive analog of sucrose was synthesized through a completely chemical synthesis route by substituting fluorine-18 (half-life 110 min) at the 6' position to generate 6'-deoxy-6'[(18)F]fluorosucrose ((18)FS). This radiotracer was then used to compare sucrose transport between wild-type maize plants and mutant plants lacking the Sucrose transporter1 (Sut1) gene, which has been shown to function in sucrose phloem loading. Our results demonstrate that (18)FS is transported in vivo, with the wild-type plants showing a greater rate of transport down the leaf blade than the sut1 mutant plants. A similar transport pattern was also observed for universally labeled [U-(14)C]sucrose ([U-(14)C]suc). Our findings support the proposed sucrose phloem loading function of the Sut1 gene in maize, and additionally demonstrate that the (18)FS analog is a valuable, new tool that offers imaging advantages over [U-(14)C]suc for studying phloem transport in plants.
authors.affiliation: Department of Chemistry, University of Missouri, Columbia, Missouri, United States of America.
authors.forename: David
authors.initials: D
authors.lastname: Rotsch
authors.affiliation: Department of Chemistry, University of Missouri, Columbia, Missouri, United States of America.
authors.forename: Tom
authors.initials: T
authors.lastname: Brossard
authors.affiliation: Division of Biological Sciences, Interdisciplinary Plant Group and the Missouri Maize Center, University of Missouri, Columbia, Missouri, United States of America.
authors.forename: Saadia
authors.initials: S
authors.lastname: Bihmidine
authors.affiliation: Department of Chemistry, University of Missouri, Columbia, Missouri, United States of America.
authors.forename: Weijiang
authors.initials: W
authors.lastname: Ying
authors.affiliation: Department of Chemistry, University of Missouri, Columbia, Missouri, United States of America.
authors.forename: Vikram
authors.initials: V
authors.lastname: Gaddam
authors.affiliation: Department of Chemistry, University of Missouri, Columbia, Missouri, United States of America.
authors.forename: Michael
authors.initials: M
authors.lastname: Harmata
authors.affiliation: Department of Chemistry, University of Missouri, Columbia, Missouri, United States of America; University of Missouri Research Reactor, University of Missouri, Columbia, Missouri, United States of America.
authors.forename: J David
authors.initials: JD
authors.lastname: Robertson
authors.affiliation: Division of Biological Sciences, Interdisciplinary Plant Group and the Missouri Maize Center, University of Missouri, Columbia, Missouri, United States of America.
authors.forename: Michael
authors.initials: M
authors.lastname: Swyers
authors.affiliation: Department of Chemistry, University of Missouri, Columbia, Missouri, United States of America.
authors.forename: Silvia S
authors.initials: SS
authors.lastname: Jurisson
authors.affiliation: Division of Biological Sciences, Interdisciplinary Plant Group and the Missouri Maize Center, University of Missouri, Columbia, Missouri, United States of America.
authors.forename: David M
authors.initials: DM
authors.lastname: Braun
mesh_terms: D001693:Biological Transport, Active / Q000502:physiology; D005462:Fluorine Radioisotopes / Q000493:pharmacokinetics / Q000494:pharmacology; D006845:Hydrocarbons, Fluorinated / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D007553:Isotope Labeling; D009004:Monosaccharide Transport Proteins / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism; D013395:Sucrose / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D003313:Zea mays / Q000378:metabolism*
chemical_list: D005462:Fluorine Radioisotopes; D006845:Hydrocarbons, Fluorinated; D009004:Monosaccharide Transport Proteins; D010940:Plant Proteins; D013395:Sucrose; C043511:6-deoxysucrose