title: Structural elucidation of a polysaccharide from Chrysanthemum morifolium flowers with anti-angiogenic activity.
abstract: The flowers of Chrysanthemum morifolium were extracted with 5% sodium hydroxide, yielding a neutral polysaccharide (JHB0S2) with a molecular weight of 16 kDa by anion-exchange chromatography on a DEAE-cellulose column and gel permeation chromatography on a Sephacryl S-200 HR column. JHB0S2 was found to contain glucose, xylose, arabinose and galactose in molar ratio of 2.9:2.3:1.0:1.2. Through the linkage analysis, partial acid hydrolysis and NMR spectra and so forth, indicated that its backbone consisted of 1,4-linked β-Glcp, nearly 50% of which was substituted at O-6 by 1,2-linked α-Xylp terminated with Galp or Xylp. Furthermore, some of 1,2-linked α-Xylp was substituted at O-4 by α-Araf-(1→5)-α-Araf-(1→6)-β-Galp. The biological test showed that JHB0S2 could inhibit the tube formation of HMEC-1 cells.
authors.affiliation: Glycobiology and Glycochemistry Laboratory, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; College of Pharmacy, Nanchang University, Nanchang 330000, China.
authors.forename: Changping
authors.initials: C
authors.lastname: Zheng
authors.affiliation: Glycobiology and Glycochemistry Laboratory, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China. Electronic address: dongqun@simm.ac.cn.
authors.forename: Qun
authors.initials: Q
authors.lastname: Dong
authors.affiliation: Glycobiology and Glycochemistry Laboratory, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
authors.forename: Zhenyun
authors.initials: Z
authors.lastname: Du
authors.affiliation: Glycobiology and Glycochemistry Laboratory, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
authors.forename: Peipei
authors.initials: P
authors.lastname: Wang
authors.affiliation: Glycobiology and Glycochemistry Laboratory, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China. Electronic address: dingkan@simm.ac.cn.
authors.forename: Kan
authors.initials: K
authors.lastname: Ding
mesh_terms: D020533:Angiogenesis Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D001089:Arabinose / Q000032:analysis; D002240:Carbohydrate Sequence; D002461:Cell Line, Transformed; D049109:Cell Proliferation / Q000187:drug effects; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D031202:Chrysanthemum / Q000737:chemistry*; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects; D035264:Flowers / Q000737:chemistry*; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006801:Humans; D006868:Hydrolysis; D008969:Molecular Sequence Data; D008970:Molecular Weight; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D012972:Sodium Hydroxide / Q000737:chemistry; D014994:Xylose / Q000032:analysis
chemical_list: D020533:Angiogenesis Inhibitors; D011134:Polysaccharides; D012972:Sodium Hydroxide; D014994:Xylose; D001089:Arabinose; D005947:Glucose; D005690:Galactose
keywords: Anti-angiogenesis; Chrysanthemum morifolium; Polysaccharide