title: Production, optimization and characterization of lactic acid by Lactobacillus delbrueckii NCIM 2025 from utilizing agro-industrial byproduct (cane molasses).
abstract: In the present work Lactobacillus delbrueckii was used to utilize agro-industrial byproduct (cane molasses) for lactic acid production under submerged fermentation process. Screening of LAB was done by Fourier transform infra red spectroscopy (FTIR). Effect of different amino acids (DL-Phenylalanine, L-Lysine and DL-Aspartic acid) on the fermentation process was done by high performance liquid chromatography (HPLC). Central composite rotatable design (CCRD) was used to optimize the levels of three parameters viz. tween 80, amino acid and cane molasses concentration during fermentative production of lactic acid. Under optimum condition lactic acid production was enhanced from 55.89 g/L to 84.50 g/L. Further, validation showed 81.50 g/L lactic acid production. Scale up was done on 7.5 L fermentor. Productivity was found to be 3.40 g/L/h which was higher than previous studies with reduced fermentation time from 24 h to 12 h. Further characterization of lactic acid was done by FTIR.
authors.affiliation: Centre of Food Science and Technology, Banaras Hindu University, Varanasi, 221 005 India.
authors.forename: Abhinay Kumar
authors.initials: AK
authors.lastname: Srivastava
authors.affiliation: Centre of Food Science and Technology, Banaras Hindu University, Varanasi, 221 005 India.
authors.forename: Abhishek Dutt
authors.initials: AD
authors.lastname: Tripathi
authors.affiliation: Centre of Food Science and Technology, Banaras Hindu University, Varanasi, 221 005 India.
authors.forename: Alok
authors.initials: A
authors.lastname: Jha
authors.affiliation: Centre of Food Science and Technology, Banaras Hindu University, Varanasi, 221 005 India.
authors.forename: Amrita
authors.initials: A
authors.lastname: Poonia
authors.affiliation: Centre of Food Science and Technology, Banaras Hindu University, Varanasi, 221 005 India.
authors.forename: Nitya
authors.initials: N
authors.lastname: Sharma
keywords: CCRD; FTIR; Lactobacillus delbrueckii; cane molasses; lactic acid; response surface methodology