title: Immuno-based detection of Shiga toxin-producing pathogenic Escherichia coli in food - A review on current approaches and potential strategies for optimization.
abstract: Certain pathogenic Escherichia coli known as Shiga toxin (Stx)-producing E. coli (STEC) are a public health threat to the consumer, and are problematic for the food industry. Food products containing STEC are deemed unfit for human consumption, and STEC illnesses can cause hemolytic uremic syndrome (HUS), a disease affecting the kidneys in susceptible individuals. Optimizing detection methods in foods have been focused on more prompt and accurate analysis. This review addresses the role and applications of immuno-based assays for STEC detection in food systems. Immunoassay antibody capture systems and flow cytometry platforms have been implemented into several food-based detection systems. By applying antibodies that will interact with target microorganisms, immunoassays can be used to directly detect and quantify pathogens. Immuno-based protocols could potentially be further implemented into the food industry, limit the duration of the detection process and increase accuracy.
authors.affiliation: a Department of Food Science , Center for Food Safety, University of Arkansas , Fayetteville , AR , USA.
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Baker
authors.affiliation: a Department of Food Science , Center for Food Safety, University of Arkansas , Fayetteville , AR , USA.
authors.forename: Peter M
authors.initials: PM
authors.lastname: Rubinelli
authors.affiliation: a Department of Food Science , Center for Food Safety, University of Arkansas , Fayetteville , AR , USA.
authors.forename: Si Hong
authors.initials: SH
authors.lastname: Park
authors.affiliation: a Department of Food Science , Center for Food Safety, University of Arkansas , Fayetteville , AR , USA.
authors.forename: Steven C
authors.initials: SC
authors.lastname: Ricke
mesh_terms: D005434:Flow Cytometry / Q000379:methods; D005516:Food Microbiology / Q000379:methods*; D007118:Immunoassay / Q000379:methods*; D054323:Shiga-Toxigenic Escherichia coli / Q000302:isolation & purification*
keywords: Detection; E. coli; flow cytometry; immunoassay