title: A highly selective and fast-response fluorescent probe for visualization of enzymatic H2S production in vitro and in living cells.
abstract: An o-fluorinated-azido-capped rhodamine probe can react with H2S efficiently and selectively to give large off-on fluorescence enhancement. The probe was used to develop an assay for cystathionine β-synthase acitivity and for in situ visualization of endogenously produced H2S in living cells.
authors.affiliation: State Key Laboratory of Elemento-Organic Chemistry and Department of Chemical Biology, National Pesticide Engineering Research Center (Tianjin), Nankai University, Tianjin, 300071, China. zhenxi@nankai.edu.cn.
authors.forename: Lv
authors.initials: L
authors.lastname: Wei
authors.forename: Zhentao
authors.initials: Z
authors.lastname: Zhu
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Li
authors.forename: Long
authors.initials: L
authors.lastname: Yi
authors.forename: Zhen
authors.initials: Z
authors.lastname: Xi
mesh_terms: D002470:Cell Survival; D003541:Cystathionine beta-Synthase / Q000378:metabolism*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D006862:Hydrogen Sulfide / Q000737:chemistry / Q000378:metabolism*; D012235:Rhodamines / Q000737:chemistry* / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D012235:Rhodamines; D003541:Cystathionine beta-Synthase; D006862:Hydrogen Sulfide