title: SufC may promote the survival of Salmonella enterica serovar Typhi in macrophages.
abstract: The sufC gene of Escherichia coli (E. coli) is required for the biogenesis of iron-sulfur (Fe-S) cluster under oxidative stress conditions. In order to investigate the roles of sufC in Salmonella enterica serovar Typhi (S. Typhi), isogenic S. Typhi strain GIFU10007 harboring a non-polar mutation of sufC (ΔsufC) was constructed and the results showed that the sufC deleted mutant grew more slowly than the wild type strain when encounter oxidative stresses. Moreover, the deletion of sufC gene decreased S. Typhi survival within macrophages. After macrophages infected by sufC deleted mutant and wild type strain, we detected IL-6 and TNF-α released into the supernatant, and found the expression of IL-6 and TNF-α decreased in the supernatant of sufC deleted mutant infected groups than the wild type strain infected ones. In summary, our results showed that SufC may promote S. Typhi coping oxidative stress and help S. Typhi survival in macrophages.
authors.affiliation: Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Qi
authors.affiliation: Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, PR China.
authors.forename: Chenhao
authors.initials: C
authors.lastname: Qin
authors.affiliation: Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, PR China.
authors.forename: Haifang
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, PR China.
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Sheng
authors.affiliation: Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, PR China. Electronic address: hong_du@126.com.
authors.forename: Hong
authors.initials: H
authors.lastname: Du
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism / Q000382:microbiology*; D050296:Microbial Viability; D018384:Oxidative Stress; D012485:Salmonella typhi / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D014435:Typhoid Fever / Q000235:genetics / Q000378:metabolism / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D000251:Adenosine Triphosphatases
keywords: Macrophage; Oxidative stress; Salmonella enterica serovar Typhi; sufC