title: Neuro-protective potential of a vesicular system of a standardized extract of a new chemotype of Withania somnifera Dunal (NMITLI118RT+) against cerebral stroke in rats.
abstract: Withania somnifera Dunal is an Indian medicinal plant with significant pharmacological properties, such as adaptogenic, anti-inflammatory, anti-oxidant, anti-platelet, anti-hypertensive, hypoglycemic and hypolipidemic effects. Several chemotypes of W. somnifera include NMITLI-101, NMITLI-118 and NMITLI-128. The present work elaborates the optimization and development of a liposomal delivery system for efficient delivery of NMITLI118RT+ [a standardized ethanolic extract of a new chemotype of W. somnifera Dunal (NMITLI-118) roots] against cerebral stroke in rats. Liposomal systems were prepared using thin-film hydration method and characterized on the basis of size, zeta potential, physical stability, FT-IR, DSC-TGA analysis and surface morphological studies by TEM. NMITLI118RT+ and its formulations (NMITLI118RT+LF) were evaluated for biological activity utilizing middle cerebral artery occlusion model in rats. The Z average of the developed liposomal formulation was about 142.6 ± 0.09 nm with a zeta potential of -31.20 ± 1.0 mV. Results of TEM revealed spherical particles in the range of 200 nm. The entrapment efficiency was found to be 94.603 ± 2%. The formulation was found to be physically stable over a 3-week period. Results were suggestive of the fact that both NMITLI118RT+ and its delivery system possess significant neuroprotective activity in cerebral ischemia. The liposomal system largely exhibits better performance over NMITLI118RT+ precisely in the post-treatment group. The present studies could elucidate the successful development of a delivery system for NMITLI118RT+ and demonstrate their beneficial neuro-protective potential in overcoming and reversing the consequences of I/R injury following stroke.
authors.affiliation: a Division of Pharmaceutics.
authors.forename: Hafsa
authors.initials: H
authors.lastname: Ahmad
authors.affiliation: a Division of Pharmaceutics.
authors.forename: Kiran
authors.initials: K
authors.lastname: Khandelwal
authors.affiliation: b Division of Pharmacology , and.
authors.forename: Sheeba Saji
authors.initials: SS
authors.lastname: Samuel
authors.affiliation: a Division of Pharmaceutics.
authors.forename: Shivangi
authors.initials: S
authors.lastname: Tripathi
authors.affiliation: c Electron Microscopy Unit , CSIR - Central Drug Research Institute , Lucknow , Uttar Pradesh , India , and.
authors.forename: Kalyan
authors.initials: K
authors.lastname: Mitra
authors.affiliation: d Department of Metabolic and Structural Biology , CSIR - Central Institute of Medicinal and Aromatic Plants , Lucknow , Uttar Pradesh , India.
authors.forename: Rajender Singh
authors.initials: RS
authors.lastname: Sangwan
authors.affiliation: b Division of Pharmacology , and.
authors.forename: Rakesh
authors.initials: R
authors.lastname: Shukla
authors.affiliation: a Division of Pharmaceutics.
authors.forename: Anil Kumar
authors.initials: AK
authors.lastname: Dwivedi
mesh_terms: D000818:Animals; D000959:Antihypertensive Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology*; D020244:Infarction, Middle Cerebral Artery / Q000188:drug therapy; D018696:Neuroprotective Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology*; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots; D051381:Rats; D017550:Spectroscopy, Fourier Transform Infrared; D020521:Stroke / Q000188:drug therapy* / Q000473:pathology; D032341:Withania / Q000737:chemistry* / Q000187:drug effects*
chemical_list: D000959:Antihypertensive Agents; D018696:Neuroprotective Agents; D010936:Plant Extracts
keywords: Ashwagandha; Withania somnifera; Withanolide A; ischemia; liposome; stroke