title: Electrophoretic concentration and sweeping-micellar electrokinetic chromatography analysis of cationic drugs in water samples.
abstract: Sample preparation by electrophoretic concentration, followed by analysis using sweeping-micellar electrokinetic chromatography, was studied as a green and simple analytical strategy for the trace analysis of cationic drugs in water samples. Electrophoretic concentration was conducted using 50 mmol/L ammonium acetate at pH 5 as acceptor electrolyte. Electrophoretic concentration was performed at 1.0 kV for 50 min and 0.5 kV and 15 min for purified and 10-fold diluted waste water samples, respectively. Sweeping-micellar electrokinetic chromatography was with 100 mmol/L sodium phosphate at pH 2, 100 mmol/L sodium dodecyl sulfate and 27.5%-v/v acetonitrile as separation electrolyte. The separation voltage was -20 kV, UV-detection was at 200 nm, and the acidified concentrate was injected for 36 s at 1 bar (or 72% of the total capillary length, 60 cm). Both purified water and 10-fold diluted waste water exhibited a linear range of two orders of concentration magnitude. The coefficient of determination, and intra- and interday repeatability were 0.991-0.997, 2.5-6.2, and 4.4-9.7%RSD (n=6), respectively, for purified water. The values were 0.991-0.997, 3.4-7.1, and 8.7-9.8%RSD (n=6), correspondingly, for 10-fold diluted waste water. The method detection limit was in the range from 0.04-0.09 to 1.20-6.97 ng/mL for purified and undiluted waste water, respectively.
authors.affiliation: Australian Centre for Research on Separation Science, School of Physical Sciences-Chemistry, University of Tasmania, Hobart, Tasmania 7001, Australia.
authors.forename: Alain
authors.initials: A
authors.lastname: Wuethrich
authors.affiliation: Australian Centre for Research on Separation Science, School of Physical Sciences-Chemistry, University of Tasmania, Hobart, Tasmania 7001, Australia.
authors.forename: Paul R
authors.initials: PR
authors.lastname: Haddad
authors.affiliation: Australian Centre for Research on Separation Science, School of Physical Sciences-Chemistry, University of Tasmania, Hobart, Tasmania 7001, Australia. Electronic address: jquirino@utas.edu.au.
authors.forename: Joselito P
authors.initials: JP
authors.lastname: Quirino
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D002412:Cations / Q000032:analysis*; D002845:Chromatography; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods; D004586:Electrophoresis; D004784:Environmental Monitoring / Q000379:methods*; D057230:Limit of Detection; D008823:Micelles; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D062065:Wastewater / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
chemical_list: D000097:Acetonitriles; D002412:Cations; D008823:Micelles; D012967:Sodium Dodecyl Sulfate; D062065:Wastewater; D014867:Water; C032159:acetonitrile
keywords: Aqueous samples; Cationic drugs; Electrophoretic concentration; Green sample preparation; MEKC; Sweeping