title: First serological and molecular evidence of PPRV occurrence in Ghardaïa district, center of Algeria.
abstract: In February 2012, an outbreak of peste des petits ruminants (PPR) was suspected in Ghardaïa district at the center of Algeria. Clinical, serological, and molecular investigations were performed to confirm the occurrence of PPRV. The overall morbidity, mortality, and case fatality rates of the ten flocks investigated were 12.2, 2.5, and 20.3 %, respectively. At the flock level, positivity to PPR was 100, 90, and 100 % by competitive ELISA (c-ELISA), RT-PCR of blood samples, and oculo-nasal swabs, respectively. At the individual levels, the present study showed that out of 186 samples collected from the same animals 17/62 (27.41 %), 14/62 (22.85 %), and 36/62 (58.06 %) were positive by c-ELISA, RT-PCR of blood samples, and RT PCR of oculo-nasal swabs, respectively. The positivity of PPR was significantly higher using RT-PCR of oculo-nasal swabs than c-ELISA and RT-PCR of blood samples. The N gene partial sequence of five PPRV-positive amplicons revealed 100 % homology among them and phylogenetically belonged to lineage IV. The sequences also showed similarity range of 97-99 % with the strains implicated in the Moroccan and Tunisian outbreaks, however, suggesting that a similar strain is circulating across this area of the Maghreb and highlighting the need for a regional control approach.
authors.affiliation: Institut National de la Médecine Vétérinaire (INMV), BP 205, Hacen Badi, El Harrach, Algiers, Algeria. drkardjadj@live.fr.|Laboratoire de Recherche "Santé et Productions Animales", Ecole Nationale Supérieure Vétérinaire d'Alger, El Alia, Algiers, Algeria. drkardjadj@live.fr.
authors.forename: Moustafa
authors.initials: M
authors.lastname: Kardjadj
authors.affiliation: Laboratoire de Recherche "Santé et Productions Animales", Ecole Nationale Supérieure Vétérinaire d'Alger, El Alia, Algiers, Algeria.
authors.forename: Meriem-Hind
authors.initials: MH
authors.lastname: Ben-Mahdi
authors.affiliation: Applied Biotechnology Division, National Veterinary Research Institute, Vom, Plateau, Nigeria.
authors.forename: Pam Dachung
authors.initials: PD
authors.lastname: Luka
mesh_terms: D000462:Algeria / Q000453:epidemiology; D000818:Animals; D004196:Disease Outbreaks / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006041:Goats; D029021:Peste-des-Petits-Ruminants / Q000097:blood / Q000453:epidemiology* / Q000821:virology; D018107:Peste-des-petits-ruminants virus / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012756:Sheep
keywords: Algeria; Ghardaïa; PPR; RT-PCR; Sequencing; Serology