title: Quantification of the chromosomal radiation damage induced by labelling of leukocytes with [18F]FDG.
abstract: BACKGROUND
  The aim of our work is to quantify the radiation damage in lymphocytes after labelling with [18F]FDG. Comparison with gold standard [99mTc]HMPAO labelling is established. An approach to cellular dosimetry is proposed.
  
  
  METHODS
  Mixed leukocytes were separated from fresh venous blood and labelled with [18F]FDG and [99mTc]HMPAO following published guidelines. Cytokinesis-block micronucleus (CBMN) assay was performed for both sets of experiments. Tests for quality control of labelling described in guidelines were followed. Cellular dosimetry was calculated according to MIRD.
  
  
  RESULTS
  MN scored after labelling with 37 MBq of [18F]FDG were 956 ± 172 and 347 ± 26 for [99mTc]HMPAO (p < 0.05). Absorbed dose in cell nucleus was of 0.23 Gy for [18F]FDG and 0.08 Gy for [99mTc]HMPAO labelling. The CBMN assay after labelling with ~290 MBq of [18F]FDG showed radiation induced inhibition of proliferation capacity of the lymphocytes, confirmed by proliferation study.
  
  
  CONCLUSIONS
  [18F]FDG labelling of mixed leukocytes causes severe radiation damage to the cell, higher than with [99mTc]HMPAO in accordance with the absorbed dose. Labelling of mixed leukocytes for clinical purpose induces high cytotoxicity reflected in the loss of proliferation capacity in lymphocytes this statement allows us to consider a low oncogenic risk however the association between MN formation in the PBL and subsequent risk of cancer is not well established.
  
  
  CONCLUSIONS
  This is the first work about radiation damage with [18F]FDG labelled cells. We focused on [18F]FDG labelling of leukocytes due to the growing number of research and review articles about this technique.
  
  
  CONCLUSIONS
  The possibility of an increased risk of lymphoid malignancies associated with the administration of radiolabelled lymphocytes is a very controversial subject. Studies on radiation damage on new labelling procedures as the one exposed in this work must be considered.
authors.affiliation: Unidad de Radiofarmacia, Hospital Universitario Virgen de la Arrixaca, Murcia, Spain. Electronic address: elenaolmo@hotmail.com.
authors.forename: Elena
authors.initials: E
authors.lastname: Miñana
authors.affiliation: Unidad de Radiofarmacia, Hospital Universitario Virgen de la Arrixaca, Murcia, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Roldán
authors.affiliation: Unidad de Radiofarmacia, Hospital Universitario Virgen de la Arrixaca, Murcia, Spain.
authors.forename: Tomás
authors.initials: T
authors.lastname: Chivato
authors.affiliation: Unidad de Radiofarmacia, Hospital General Universitario Santa Lucia, Cartagena Spain. Electronic address: tmmtnez@gmail.com.
authors.forename: Teresa
authors.initials: T
authors.lastname: Martínez
authors.affiliation: Unidad de Radiofarmacia, Hospital Universitario Virgen de la Arrixaca, Murcia, Spain.
authors.forename: Teodomiro
authors.initials: T
authors.lastname: Fuente
mesh_terms: D000328:Adult; D000368:Aged; D001681:Biological Assay / Q000379:methods; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005260:Female; D019788:Fluorodeoxyglucose F18; D006801:Humans; D007553:Isotope Labeling / Q000379:methods; D007962:Leukocytes / Q000000981:diagnostic imaging* / Q000502:physiology / Q000528:radiation effects*; D008297:Male; D015162:Micronucleus Tests / Q000379:methods*; D008875:Middle Aged; D049268:Positron-Emission Tomography / Q000379:methods*; D011829:Radiation Dosage; D011874:Radiometry / Q000379:methods; D019275:Radiopharmaceuticals
chemical_list: D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18
keywords: CBMN assay; Cellular dosimetry; Cytotoxicity; Human leukocytes; Radiation damage; [18F]FDG