title: Enhanced Control of Bladder-Associated Tumors Using Shrimp Anti-Lipopolysaccharide Factor (SALF) Antimicrobial Peptide as a Cancer Vaccine Adjuvant in Mice.
abstract: Shrimp anti-lipopolysaccharide factor (SALF) is an antimicrobial peptide with reported anticancer activities, such as suppression of tumor progression. In this study, we prepared a potential cancer vaccine comprised of SALF in conjunction with the cell lysate of inactivated murine bladder carcinoma cells (MBT-2), and evaluated its efficacy in a mouse tumor model. Our study shows that SALF added to cell culture media inhibits growth progression of MBT-2, and that SALF together with inactivated MBT-2 lysate elevates the level of inflammasome activity, and modulates the levels of IL-1β, MCP-1, IL-6, IL-12, and TNF-α in mouse macrophages. Immunization of 7, 14, and 21 day-old mice with the vaccine prevented growth of MBT-2 cell-mediated tumors. The vaccine was found to enhance expression of T-cell, cytotoxic T cells, and NK cells in the immunized mice groups. Recruitment of macrophages, T-helper cells, and NK cells was enhanced, but levels of VEGF were decreased in immunized mice. This report provides empirical evidence that our SALF as vaccine adjuvant enhances antitumor immunity in mice.
authors.affiliation: Department of Food Science, National Taiwan Ocean University, Keelung 202, Taiwan. henryhw910@yahoo.com.tw.
authors.forename: Han-Ning
authors.initials: HN
authors.lastname: Huang
authors.affiliation: Marine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica, 23-10 Dahuen Road, Jiaushi, Ilan 262, Taiwan. ratcatflat@yahoo.com.
authors.forename: Venugopal
authors.initials: V
authors.lastname: Rajanbabu
authors.affiliation: Department and Graduate Institute of Aquaculture, National Kaohsiung Marine University, Kaohsiung 811, Taiwan. panjade@webmail.nkmu.edu.tw.
authors.forename: Chieh-Yu
authors.initials: CY
authors.lastname: Pan
authors.affiliation: Department of Life Science, Chinese Culture University, Taipei 111, Taiwan. zyl13@ulive.pccu.edu.tw.
authors.forename: Yi-Lin
authors.initials: YL
authors.lastname: Chan
authors.affiliation: Marine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica, 23-10 Dahuen Road, Jiaushi, Ilan 262, Taiwan. zoocjy@gate.sinica.edu.tw.
authors.forename: Jyh-Yih
authors.initials: JY
authors.lastname: Chen
authors.affiliation: Department of Food Science, National Taiwan Ocean University, Keelung 202, Taiwan. cjwu@mail.ntou.edu.tw.
authors.forename: Chang-Jer
authors.initials: CJ
authors.lastname: Wu
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000276:immunology; D023181:Antimicrobial Cationic Peptides / Q000276:immunology*; D060829:Arthropod Proteins / Q000276:immunology*; D019496:Cancer Vaccines / Q000276:immunology*; D045744:Cell Line, Tumor; D018932:Chemokine CCL2 / Q000276:immunology; D007378:Interleukins / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology*; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008809:Mice, Inbred C3H; D010455:Peptides / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology; D001749:Urinary Bladder Neoplasms / Q000276:immunology*; D042461:Vascular Endothelial Growth Factor A / Q000276:immunology
chemical_list: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D060829:Arthropod Proteins; D019496:Cancer Vaccines; C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; D007378:Interleukins; D008070:Lipopolysaccharides; D010455:Peptides; D014409:Tumor Necrosis Factor-alpha; D042461:Vascular Endothelial Growth Factor A; C034705:antilipopolysaccharide factor (Limulus)
keywords: MBT-2; SALF; antimicrobial peptide; bladder tumor; cancer vaccine