title: Hippocampal spine changes across the sleep-wake cycle: corticosterone and kinases.
abstract: The corticosterone (CORT) level changes along the circadian rhythm. Hippocampus is sensitive to CORT, since glucocorticoid receptors are highly expressed. In rat hippocampus fixed in a living state every 3 h, we found that the dendritic spine density of CA1 pyramidal neurons increased upon waking (within 3 h), as compared with the spine density in the sleep state. Particularly, the large-head spines increased. The observed change in the spine density may be due to the change in the hippocampal CORT level, since the CORT level at awake state (∼30 nM) in cerebrospinal fluid was higher than that at sleep state (∼3 nM), as observed from our earlier study. In adrenalectomized (ADX) rats, such a wake-induced increase of the spine density disappeared. S.c. administration of CORT into ADX rats rescued the decreased spine density. By using isolated hippocampal slices, we found that the application of 30 nM CORT increased the spine density within 1 h and that the spine increase was mediated via PKA, PKC, ERK MAPK, and LIMK signaling pathways. These findings suggest that the moderately rapid increase of the spine density on waking might mainly be caused by the CORT-driven kinase networks.
authors.affiliation: Department of Biophysics and Life SciencesGraduate School of Arts and Sciences, University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 152-8902, JapanBioinformatics Project of Japan Science and Technology AgencyUniversity of Tokyo, Tokyo, JapanLaboratory of Exercise Biochemistry and NeuroendocrinologyFaculty of Health and Sports Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, JapanDepartment of UrologyGraduate School of Medicine, Juntendo University, 2-1-1 Hongo, Tokyo 113-8424, Japan.
authors.forename: Muneki
authors.initials: M
authors.lastname: Ikeda
authors.affiliation: Department of Biophysics and Life SciencesGraduate School of Arts and Sciences, University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 152-8902, JapanBioinformatics Project of Japan Science and Technology AgencyUniversity of Tokyo, Tokyo, JapanLaboratory of Exercise Biochemistry and NeuroendocrinologyFaculty of Health and Sports Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, JapanDepartment of UrologyGraduate School of Medicine, Juntendo University, 2-1-1 Hongo, Tokyo 113-8424, Japan Department of Biophysics and Life SciencesGraduate School of Arts and Sciences, University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 152-8902, JapanBioinformatics Project of Japan Science and Technology AgencyUniversity of Tokyo, Tokyo, JapanLaboratory of Exercise Biochemistry and NeuroendocrinologyFaculty of Health and Sports Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, JapanDepartment of UrologyGraduate School of Medicine, Juntendo University, 2-1-1 Hongo, Tokyo 113-8424, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Hojo
authors.affiliation: Department of Biophysics and Life SciencesGraduate School of Arts and Sciences, University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 152-8902, JapanBioinformatics Project of Japan Science and Technology AgencyUniversity of Tokyo, Tokyo, JapanLaboratory of Exercise Biochemistry and NeuroendocrinologyFaculty of Health and Sports Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, JapanDepartment of UrologyGraduate School of Medicine, Juntendo University, 2-1-1 Hongo, Tokyo 113-8424, Japan.
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Komatsuzaki
authors.affiliation: Department of Biophysics and Life SciencesGraduate School of Arts and Sciences, University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 152-8902, JapanBioinformatics Project of Japan Science and Technology AgencyUniversity of Tokyo, Tokyo, JapanLaboratory of Exercise Biochemistry and NeuroendocrinologyFaculty of Health and Sports Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, JapanDepartment of UrologyGraduate School of Medicine, Juntendo University, 2-1-1 Hongo, Tokyo 113-8424, Japan Department of Biophysics and Life SciencesGraduate School of Arts and Sciences, University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 152-8902, JapanBioinformatics Project of Japan Science and Technology AgencyUniversity of Tokyo, Tokyo, JapanLaboratory of Exercise Biochemistry and NeuroendocrinologyFaculty of Health and Sports Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, JapanDepartment of UrologyGraduate School of Medicine, Juntendo University, 2-1-1 Hongo, Tokyo 113-8424, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Okamoto
authors.affiliation: Department of Biophysics and Life SciencesGraduate School of Arts and Sciences, University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 152-8902, JapanBioinformatics Project of Japan Science and Technology AgencyUniversity of Tokyo, Tokyo, JapanLaboratory of Exercise Biochemistry and NeuroendocrinologyFaculty of Health and Sports Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, JapanDepartment of UrologyGraduate School of Medicine, Juntendo University, 2-1-1 Hongo, Tokyo 113-8424, Japan.
authors.forename: Asami
authors.initials: A
authors.lastname: Kato
authors.affiliation: Department of Biophysics and Life SciencesGraduate School of Arts and Sciences, University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 152-8902, JapanBioinformatics Project of Japan Science and Technology AgencyUniversity of Tokyo, Tokyo, JapanLaboratory of Exercise Biochemistry and NeuroendocrinologyFaculty of Health and Sports Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, JapanDepartment of UrologyGraduate School of Medicine, Juntendo University, 2-1-1 Hongo, Tokyo 113-8424, Japan.
authors.forename: Taishi
authors.initials: T
authors.lastname: Takeda
authors.affiliation: Department of Biophysics and Life SciencesGraduate School of Arts and Sciences, University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 152-8902, JapanBioinformatics Project of Japan Science and Technology AgencyUniversity of Tokyo, Tokyo, JapanLaboratory of Exercise Biochemistry and NeuroendocrinologyFaculty of Health and Sports Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, JapanDepartment of UrologyGraduate School of Medicine, Juntendo University, 2-1-1 Hongo, Tokyo 113-8424, Japan Department of Biophysics and Life SciencesGraduate School of Arts and Sciences, University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 152-8902, JapanBioinformatics Project of Japan Science and Technology AgencyUniversity of Tokyo, Tokyo, JapanLaboratory of Exercise Biochemistry and NeuroendocrinologyFaculty of Health and Sports Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, JapanDepartment of UrologyGraduate School of Medicine, Juntendo University, 2-1-1 Hongo, Tokyo 113-8424, Japan Department of Biophysics and Life SciencesGraduate School of Arts and Sciences, University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 152-8902, JapanBioinformatics Project of Japan Science and Technology AgencyUniversity of Tokyo, Tokyo, JapanLaboratory of Exercise Biochemistry and NeuroendocrinologyFaculty of Health and Sports Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, JapanDepartment of UrologyGraduate School of Medicine, Juntendo University, 2-1-1 Hongo, Tokyo 113-8424, Japan kawato@bio.c.u-tokyo.ac.jp.
authors.forename: Suguru
authors.initials: S
authors.lastname: Kawato
mesh_terms: D000818:Animals; D048430:Cell Shape / Q000502:physiology; D002940:Circadian Rhythm / Q000502:physiology*; D003345:Corticosterone / Q000378:metabolism* / Q000494:pharmacology; D049229:Dendritic Spines / Q000187:drug effects / Q000378:metabolism*; D006624:Hippocampus / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology
chemical_list: D003345:Corticosterone
keywords: brain; circadian rhythms; corticosteroids; memory; neuroendocrinology