title: Approaches to identifying synthetic lethal interactions in cancer.
abstract: Targeting synthetic lethal interactions is a promising new therapeutic approach to exploit specific changes that occur within cancer cells. Multiple approaches to investigate these interactions have been developed and successfully implemented, including chemical, siRNA, shRNA, and CRISPR library screens. Genome-wide computational approaches, such as DAISY, also have been successful in predicting synthetic lethal interactions from both cancer cell lines and patient samples. Each approach has its advantages and disadvantages that need to be considered depending on the cancer type and its molecular alterations. This review discusses these approaches and examines case studies that highlight their use.
authors.affiliation: Molecular Biology and Biochemistry Department, University of California Irvine, Irvine, California.
authors.forename: Jordan M
authors.initials: JM
authors.lastname: Thompson
authors.affiliation: Molecular Biology and Biochemistry Department, University of California Irvine, Irvine, California.
authors.forename: Quy H
authors.initials: QH
authors.lastname: Nguyen
authors.affiliation: Molecular Biology and Biochemistry Department, University of California Irvine, Irvine, California.
authors.forename: Manpreet
authors.initials: M
authors.lastname: Singh
authors.affiliation: Molecular Biology and Biochemistry Department, University of California Irvine, Irvine, California.
authors.forename: Olga V
authors.initials: OV
authors.lastname: Razorenova
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects*; D055808:Drug Discovery; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D058990:Molecular Targeted Therapy / Q000379:methods*; D009369:Neoplasms / Q000473:pathology / Q000503:physiopathology* / Q000628:therapy*
chemical_list: D000970:Antineoplastic Agents
keywords: CRISPR library; CRISPR screen; DAISY; antitumor; cancer; computational screen; drug discovery; drug screening assays; library screens; neoplasms; shRNA library; shRNA screen; siRNA library; siRNA screen; synthetic lethal interactions; synthetic lethality