title: A functional comparison of the domestic cat bitter receptors Tas2r38 and Tas2r43 with their human orthologs.
abstract: BACKGROUND
  Domestic cats (felis catus) have a reputation for being rather unpredictable in their dietary choices. While their appetite for protein or savory flavors is consistent with their nutritional needs, their preference among protein-sufficient dietary options may relate to differences in the response to other flavor characteristics. Studies of domestic cat taste perception are limited, in part, due to the lack of receptor sequence information. Several studies have described the phylogenetic relationship of specific cat taste receptor sequences as compared with other carnivores. For example, domestic cats are obligate carnivores and their receptor Tas1r2, associated with the human perception of sweet, is present only as a pseudogene. Similarly, the cat perception of bitter may differ from that of other mammals due to variations in their repertoire of bitter receptor (Tas2r) genes. This report includes the first functional characterization of domestic cat taste receptors.
  
  
  RESULTS
  We functionally expressed two uncharacterized domestic sequences Tas2r38 and Tas2r43 and deorphanized the receptors using a cellular functional assay. Statistical significance was determined using an unpaired, two-tailed t-test. The cat sequence for Tas2r38 contains 3 major amino acid residues known to confer the taster phenotype (PAI), which is associated with sensitivity to the bitter compounds PROP and PTC. However, in contrast to human TAS2R38, cat Tas2r38 is activated by PTC but not by PROP. Furthermore, like its human counterpart, cat Tas2r43 is activated by aloin and denatonium, but differs from the human TAS2R43 by insensitivity to saccharin. The responses of both cat receptors to the bitter ligands were concentration-dependent and were inhibited by the human bitter blocker probenecid.
  
  
  CONCLUSIONS
  These data demonstrate that the response profiles of the cat bitter receptors Tas2r38 and Tas2r43 are distinct from those of their orthologous human receptors. Results with cat Tas2r38 also demonstrate that additional residues beyond those classically associated with PROP sensitivity in humans influence the sensitivity to PROP and PTC. Functional studies of the human bitter receptor family are being applied to the development of food and medicinal products with more appealing flavor profiles. Our work lays the foundation for similar work applied to felines.
authors.affiliation: AFB International, St. Charles, MO, USA. msandau@afbinternational.com.
authors.forename: Michelle M
authors.initials: MM
authors.lastname: Sandau
authors.affiliation: Integral Molecular, Inc., Philadelphia, PA, USA. jrg5032@gmail.com.
authors.forename: Jason R
authors.initials: JR
authors.lastname: Goodman
authors.affiliation: Integral Molecular, Inc., Philadelphia, PA, USA. athomas@integralmolecular.com.
authors.forename: Anu
authors.initials: A
authors.lastname: Thomas
authors.affiliation: Integral Molecular, Inc., Philadelphia, PA, USA. jrucker@integralmolecular.com.
authors.forename: Joseph B
authors.initials: JB
authors.lastname: Rucker
authors.affiliation: AFB International, St. Charles, MO, USA. nrawson@afbinternational.com.
authors.forename: Nancy E
authors.initials: NE
authors.lastname: Rawson
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002415:Cats; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005455:Fluorescent Antibody Technique; D006801:Humans; D011339:Probenecid / Q000494:pharmacology; D043562:Receptors, G-Protein-Coupled / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D018689:Sensory System Agents / Q000494:pharmacology; D013045:Species Specificity; D014162:Transfection; D000098706:Taste Receptors, Type 2
chemical_list: D043562:Receptors, G-Protein-Coupled; D018689:Sensory System Agents; D000098706:Taste Receptors, Type 2; D011339:Probenecid; D002118:Calcium