title: Immune responses and protection induced by Brucella suis S2 bacterial ghosts in mice.
abstract: With the purpose of generating Brucella suis bacterial ghosts and investigating the immunogenicity of bacterial ghosts as a vaccine candidate, the lysis gene E and temperature-sensitive regulator cassette were cloned into a shuttle plasmid, pBBR1MCS-2, for construction of a recombinant temperature-sensitive shuttle lysis plasmid, pBBR1MCS-E. pBBR1MCS-E was then introduced into attenuated B. suis live vaccine S2 bacteria, and the resultant transformants were used for production of B. suis ghosts (BSGs) by inducing lysis gene E expression. The BSGs were characterized by observing their morphology by transmission electron microscopy. The safety and immunogenicity of BSGs were further evaluated using a murine model, the result suggested that BSG was as safe as formalin-killed B. suis. In mice, BSG demonstrated a similar capacity of inducing pathogen-specific serum IgG antibody response, spleen CD3(+) and CD4(+) T cell responses, induce secretion of gamma interferon and interleukin-4, and protection levels against Brucella melitensis 16M challenge, as the attenuated B. suis live vaccine. These data suggesting that BSG could confer protection against Brucella infection in a mouse model of disease and may be developed as a new vaccine candidate against Brucella infection.
authors.affiliation: Institute of Military Veterinary Science, Academy of Military Medical Sciences, Changchun 130122, China. Electronic address: liujunbio@yahoo.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.affiliation: Hospital of Stomatology, Jilin University, Changchun 130021, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute of Military Veterinary Science, Academy of Military Medical Sciences, Changchun 130122, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Institute of Military Veterinary Science, Academy of Military Medical Sciences, Changchun 130122, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Ji
authors.affiliation: Institute of Military Veterinary Science, Academy of Military Medical Sciences, Changchun 130122, China.
authors.forename: Lingwei
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Institute of Military Veterinary Science, Academy of Military Medical Sciences, Changchun 130122, China.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Guo
authors.affiliation: Institute of Military Veterinary Science, Academy of Military Medical Sciences, Changchun 130122, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Institute of Military Veterinary Science, Academy of Military Medical Sciences, Changchun 130122, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhou
authors.affiliation: Institute of Military Veterinary Science, Academy of Military Medical Sciences, Changchun 130122, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Liu
authors.affiliation: Institute of Military Veterinary Science, Academy of Military Medical Sciences, Changchun 130122, China.
authors.forename: Ruian
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Institute of Military Veterinary Science, Academy of Military Medical Sciences, Changchun 130122, China. Electronic address: shuzhangf@yahoo.com.
authors.forename: Shuzhang
authors.initials: S
authors.lastname: Feng
mesh_terms: D000818:Animals; D002004:Brucella Vaccine / Q000627:therapeutic use*; D041021:Brucella suis / Q000276:immunology*; D002006:Brucellosis / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D005260:Female; D005434:Flow Cytometry; D007110:Immunity, Active / Q000276:immunology; D007111:Immunity, Cellular / Q000276:immunology; D056724:Immunity, Humoral / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D046529:Microscopy, Electron, Transmission; D016176:T-Lymphocyte Subsets / Q000276:immunology
chemical_list: D002004:Brucella Vaccine
keywords: Bacterial ghosts; Brucella suis; Immune protection; Vaccine