title: Development of Primer Sets for Loop-Mediated Isothermal Amplification that Enables Rapid and Specific Detection of Streptococcus dysgalactiae, Streptococcus uberis and Streptococcus agalactiae.
abstract: Streptococcus dysgalactiae, Streptococcus uberis and Streptococcus agalactiae are the three main pathogens causing bovine mastitis, with great losses to the dairy industry. Rapid and specific loop-mediated isothermal amplification methods (LAMP) for identification and differentiation of these three pathogens are not available. With the 16S rRNA gene and 16S-23S rRNA intergenic spacers as targets, four sets of LAMP primers were designed for identification and differentiation of S. dysgalactiae, S. uberis and S. agalactiae. The detection limit of all four LAMP primer sets were 0.1 pg DNA template per reaction, the LAMP method with 16S rRNA gene and 16S-23S rRNA intergenic spacers as the targets can differentiate the three pathogens, which is potentially useful in epidemiological studies.
authors.affiliation: Henan Postdoctoral Research Base, Food and Bioengineering College, Xuchang University, Xuchang 461000, China. wangdg666@aliyun.com.
authors.forename: Deguo
authors.initials: D
authors.lastname: Wang
authors.affiliation: Molecular Characterization of Foodborne Pathogens Research Unit, Eastern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA. yanhong.liu@ars.usda.gov.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D002417:Cattle; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis*; D005260:Female; D008414:Mastitis, Bovine / Q000382:microbiology*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000032:analysis*; D013291:Streptococcus / Q000235:genetics / Q000302:isolation & purification*; D013292:Streptococcus agalactiae / Q000235:genetics / Q000302:isolation & purification
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: 16S rRNA gene; 16S-23S rRNA intergenic spacer; Streptococcus agalactiae; Streptococcus dysgalactiae; Streptococcus uberis; loop-mediated isothermal amplification (LAMP)