title: In vitro effect of ursolic acid on the inhibition of Mycobacterium tuberculosis and its cell wall mycolic acid.
abstract: Mycobacterium tuberculosis is a dangerous intracellular pathogen. In order to protect against mycobacterium infection, novel agents with anti-mycobacterial activity should be given emergency priority for evaluation. Ursolic acid (UA), a plant triterpenoid, shows promising bioactivities, including anti-mycobacterial potency. In this study, the action of UA against Mycobacterium tuberculosis H37Ra was evaluated, and the inhibitory concentration was found to range between 10 and 20 μg/ml in a resazurin assay and MGIT 960 instrument. The total mycolic acid in UA-treated H37Ra was detected and compared with INH-treated and non-treated bacterium by LC-MS/MS. Quantitative LC-MS/MS data confirmed that both UA and INH decreased mycolic acid biosynthesis in a dose-dependent manner. Thin-layer chromatogram (TLC) analysis showed that all mycolic acid subtypes were affected by UA treatment in the wild type but not in strains resistant to UA. Electron microscopy images also confirmed that UA treatment affected both H37Ra cell and intracellular content of H37Ra. Altogether, these data confirmed the promise of the inhibitory action of UA in mycolic acid, which might further delineate the mechanistic pathway of mycobacterial inhibition by UA.
authors.affiliation: Department of Microbiology and Immunology, Soonchunhyang University, 8 Soonchuhyang 2-gil, Cheonan si 330-721, Republic of Korea.
authors.forename: Md Anirban
authors.initials: MA
authors.lastname: Jyoti
authors.affiliation: Department of Microbiology and Immunology, Soonchunhyang University, 8 Soonchuhyang 2-gil, Cheonan si 330-721, Republic of Korea.
authors.forename: Tamanna
authors.initials: T
authors.lastname: Zerin
authors.affiliation: Bioresourses Regional Innovation Center, Soonchunhyang University, Asan, Republic of Korea.
authors.forename: Tae-Hyun
authors.initials: TH
authors.lastname: Kim
authors.affiliation: Department of Microbiology and Immunology, Soonchunhyang University, 8 Soonchuhyang 2-gil, Cheonan si 330-721, Republic of Korea.
authors.forename: Tae-Seon
authors.initials: TS
authors.lastname: Hwang
authors.affiliation: Department of Microbiology and Immunology, Soonchunhyang University, 8 Soonchuhyang 2-gil, Cheonan si 330-721, Republic of Korea.
authors.forename: Woong Sik
authors.initials: WS
authors.lastname: Jang
authors.affiliation: Department of Chemistry, Soonchunhyang University, 22 Soonchunhyang-ro, Sinchangmyeon, Asan, Chungcheonam 336-745, Republic of Korea.
authors.forename: Kung-Woo
authors.initials: KW
authors.lastname: Nam
authors.affiliation: Department of Microbiology and Immunology, Soonchunhyang University, 8 Soonchuhyang 2-gil, Cheonan si 330-721, Republic of Korea. Electronic address: songmic@sch.ac.kr.
authors.forename: Ho-Yeon
authors.initials: HY
authors.lastname: Song
mesh_terms: D000995:Antitubercular Agents / Q000008:administration & dosage / Q000494:pharmacology*; D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods; D004305:Dose-Response Relationship, Drug; D008826:Microbial Sensitivity Tests; D008854:Microscopy, Electron; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D009171:Mycolic Acids / Q000378:metabolism*; D053719:Tandem Mass Spectrometry / Q000379:methods; D014315:Triterpenes / Q000008:administration & dosage / Q000494:pharmacology*; D000097245:Ursolic Acid
chemical_list: D000995:Antitubercular Agents; D009171:Mycolic Acids; D014315:Triterpenes; D000097245:Ursolic Acid
keywords: H37Ra; LC-MS/MS; Mycobacterium tuberculosis; Mycolic acid; Ursolic acid