title: Characteristics of the Localization of Connexin 43 in Satellite Cells during Skeletal Muscle Regeneration In Vivo.
abstract: For myogenesis, new myotubes are formed by the fusion of differentiated myoblasts. In the sequence of events for myotube formation, intercellular communication through gap junctions composed of connexin 43 (Cx43) plays critical roles in regulating the alignment and fusion of myoblasts in advances of myotube formation in vitro. On the other hand, the relationship between the expression patterns of Cx43 and the process of myotube formation in satellite cells during muscle regeneration in vivo remains poorly understood. The present study investigated the relationship between Cx43 and satellite cells in muscle regeneration in vivo. The expression of Cx43 was detected in skeletal muscles on day 1 post-muscle injury, but not in control muscles. Interestingly, the expression of Cx43 was not localized on the inside of the basement membrane of myofibers in the regenerating muscles. Moreover, although the clusters of differentiated satellite cells, which represent a more advanced stage of myotube formation, were observed on the inside of the basement membrane of myofibers in regenerating muscles, the expression of Cx43 was not localized in the clusters of these satellite cells. Therefore, in the present study, it was suggested that Cx43 may not directly contribute to muscle regeneration via satellite cells.
authors.affiliation: Section for Health-related Physical Education, Division of Human Sciences, Faculty of Engineering, Osaka Institute of Technology , Osaka 535-8585, Japan.
authors.forename: Minenori
authors.initials: M
authors.lastname: Ishido
authors.affiliation: Department of Physical Education, Aichi University of Education , Aichi 448-0001, Japan.
authors.forename: Norikatsu
authors.initials: N
authors.lastname: Kasuga
keywords: connexin 43; in vivo; muscle regeneration; satellite cells