title: Plant Translation Elongation Factor 1Bβ Facilitates Potato Virus X (PVX) Infection and Interacts with PVX Triple Gene Block Protein 1.
abstract: The eukaryotic translation elongation factor 1 (eEF1) has two components: the G-protein eEF1A and the nucleotide exchange factor eEF1B. In plants, eEF1B is itself composed of a structural protein (eEF1Bγ) and two nucleotide exchange subunits (eEF1Bα and eEF1Bβ). To test the effects of elongation factors on virus infection, we isolated eEF1A and eEF1B genes from pepper (Capsicum annuum) and suppressed their homologs in Nicotiana benthamiana using virus-induced gene silencing (VIGS). The accumulation of a green fluorescent protein (GFP)-tagged Potato virus X (PVX) was significantly reduced in the eEF1Bβ- or eEF1Bɣ-silenced plants as well as in eEF1A-silenced plants. Yeast two-hybrid and co-immunoprecipitation analyses revealed that eEF1Bα and eEF1Bβ interacted with eEF1A and that eEF1A and eEF1Bβ interacted with triple gene block protein 1 (TGBp1) of PVX. These results suggest that both eEF1A and eEF1Bβ play essential roles in the multiplication of PVX by physically interacting with TGBp1. Furthermore, using eEF1Bβ deletion constructs, we found that both N- (1-64 amino acids) and C-terminal (150-195 amino acids) domains of eEF1Bβ are important for the interaction with PVX TGBp1 and that the C-terminal domain of eEF1Bβ is involved in the interaction with eEF1A. These results suggest that eEF1Bβ could be a potential target for engineering virus-resistant plants.
authors.affiliation: Department of Plant Science, Plant Genomics & Breeding Institute, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Korea; Korea Institute of Science and Technology Information, Seoul, 130-741, Korea.
authors.forename: JeeNa
authors.initials: J
authors.lastname: Hwang
authors.affiliation: Department of Plant Science, Plant Genomics & Breeding Institute, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Korea.
authors.forename: Seonhee
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Plant Science, Plant Genomics & Breeding Institute, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Korea.
authors.forename: Joung-Ho
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Plant Science, Plant Genomics & Breeding Institute, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Korea.
authors.forename: Won-Hee
authors.initials: WH
authors.lastname: Kang
authors.affiliation: Department of Plant Science, Plant Genomics & Breeding Institute, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Korea; Crop Biotechnology Institute/GreenBio Science and Technology, Seoul National University, Pyeongchang, 232-916, Korea.
authors.forename: Jin-Ho
authors.initials: JH
authors.lastname: Kang
authors.affiliation: Department of Plant Science, Plant Genomics & Breeding Institute, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Korea.
authors.forename: Min-Young
authors.initials: MY
authors.lastname: Kang
authors.affiliation: Department of Horticultural Biotechnology, Kyung Hee University, Yongin, 446-701, Korea.
authors.forename: Chang-Sik
authors.initials: CS
authors.lastname: Oh
authors.affiliation: Department of Plant Science, Plant Genomics & Breeding Institute, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Korea; Crop Biotechnology Institute/GreenBio Science and Technology, Seoul National University, Pyeongchang, 232-916, Korea.
authors.forename: Byoung-Cheorl
authors.initials: BC
authors.lastname: Kang
mesh_terms: D002212:Capsicum / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D060467:Disease Resistance; D020648:Peptide Elongation Factor 1 / Q000235:genetics / Q000378:metabolism*; D010935:Plant Diseases / Q000821:virology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D017863:Potexvirus / Q000235:genetics / Q000378:metabolism*; D020365:RNA Helicases / Q000235:genetics / Q000378:metabolism*; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D020648:Peptide Elongation Factor 1; D010940:Plant Proteins; D014764:Viral Proteins; D020365:RNA Helicases