title: Feces of feedlot cattle contain a diversity of bacteriophages that lyse non-O157 Shiga toxin-producing Escherichia coli.
abstract: This study aimed to isolate and characterize bacteriophages that lyse non-O157 Shiga toxin-producing Escherichia coli (STEC) from cattle feces. Of 37 non-O157 STEC-infecting phages isolated, those targeting O26 (AXO26A, AYO26A, AYO26B), O103 (AXO103A, AYO103A), O111 (AXO111A, AYO111A), O121 (AXO121A, AXO121B), and O145 (AYO145A, AYO145B) were further characterized. Transmission electron microscopy showed that the 11 isolates belonged to 3 families and 6 genera: the families Myoviridae (types rV5, T4, ViI, O1), Siphoviridae (type T5), and Podoviridae (type T7). Genome size of the phages as determined by pulsed-field gel electrophoresis ranged from 38 to 177 kb. Excluding phages AXO26A, AYO103A, AYO145A, and AYO145B, all other phages were capable of lysing more than 1 clinically important strain from serogroups of O26, O91, O103, O111, O113, O121, and O128, but none exhibited infectivity across all serogroups. Moreover, phages AYO26A, AXO121A, and AXO121B were also able to lyse 4 common phage types of STEC O157:H7. Our findings show that a diversity of non-O157 STEC-infecting phages are harbored in bovine feces. Phages AYO26A, AYO26B, AXO103A, AXO111A, AYO111A, AXO121A, and AXO121B exhibited a broad host range against a number of serogroups of STEC and have potential for the biocontrol of STEC in the environment.
authors.affiliation: a College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong 510642, People's Republic of China.|c Agriculture and Agri-Food Canada, Lethbridge, AB T1J 4B1, Canada.
authors.forename: Jiaying
authors.initials: J
authors.lastname: Wang
authors.affiliation: b Alberta Agriculture and Rural Development, Lethbridge, AB T1J 4V6, Canada.
authors.forename: Yan D
authors.initials: YD
authors.lastname: Niu
authors.affiliation: a College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong 510642, People's Republic of China.
authors.forename: Jinding
authors.initials: J
authors.lastname: Chen
authors.affiliation: d Department of Food Science, Canadian Research Institute for Food Safety, Guelph, ON N1G 2W1, Canada.|e Department of Microbiology, Faculty of Science, Ain Shams University, Cairo, Egypt.
authors.forename: Hany
authors.initials: H
authors.lastname: Anany
authors.affiliation: f Département de microbiologie, Faculté de médecine, Université Laval, Québec, QC G1K 7P4, Canada.
authors.forename: Hans-W
authors.initials: HW
authors.lastname: Ackermann
authors.affiliation: g Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Guelph, ON N1G 3W4, Canada.
authors.forename: Roger P
authors.initials: RP
authors.lastname: Johnson
authors.affiliation: h Department of Biological Sciences, Faculty of Agriculture Science and Technology, North West University-Mafikeng Campus, North West, Mmabatho 2735, South Africa.
authors.forename: Collins N
authors.initials: CN
authors.lastname: Ateba
authors.affiliation: b Alberta Agriculture and Rural Development, Lethbridge, AB T1J 4V6, Canada.
authors.forename: Kim
authors.initials: K
authors.lastname: Stanford
authors.affiliation: c Agriculture and Agri-Food Canada, Lethbridge, AB T1J 4B1, Canada.
authors.forename: Tim A
authors.initials: TA
authors.lastname: McAllister
mesh_terms: D000818:Animals; D001435:Bacteriophages / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology*; D044822:Biodiversity; D002417:Cattle; D016521:Electrophoresis, Gel, Pulsed-Field; D005243:Feces / Q000382:microbiology* / Q000821:virology*; D058507:Host Specificity; D017900:Myoviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D054323:Shiga-Toxigenic Escherichia coli / Q000235:genetics / Q000302:isolation & purification / Q000821:virology*; D017906:Siphoviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*
keywords: Escherichia coli; bacteriophages; bactériophages; biocontrol; biocontrôle; bovins; cattle; non-O157