title: Detection and characterization of OXA-48-producing Klebsiella pneumoniae originated in Bulgaria.
abstract: We report the identification of OXA-48-producing Klebsiella pneumoniae, causing peritonitis in a cancer patient admitted to the Oncology Hospital in Sofia. The isolate had reduced susceptibility to carbapenems but remained susceptible to extended-spectrum cephalosporins. PCR and sequencing confirmed the presence of blaOXA-48 gene flanked by two intact copies of IS1999 on truncated ΔTn1999.1. This transposon was located on unusual non-typeable 29-kb plasmid that could be transferred only by transformation. Multilocus sequence typing (MLST) indicated the presence of the sequence type ST530.This is the first documented infection due to OXA-48-producing Enterobacteriaceae strain in Bulgaria.
authors.affiliation: a Laboratory for Clinical Microbiology , National Oncology Center - SHATO , Sofia , Bulgaria.
authors.forename: Stefana
authors.initials: S
authors.lastname: Sabtcheva
authors.affiliation: b National Reference Laboratory for Control and Monitoring of Antibiotic Resistance , Department of Microbiology, National Center of Infectious and Parasitic Diseases , Bulgaria.
authors.forename: Ivan N
authors.initials: IN
authors.lastname: Ivanov
authors.affiliation: b National Reference Laboratory for Control and Monitoring of Antibiotic Resistance , Department of Microbiology, National Center of Infectious and Parasitic Diseases , Bulgaria.
authors.forename: Bozhana
authors.initials: B
authors.lastname: Todorova
authors.affiliation: c Department of Surgery , Specialized Hospital for Active Treatment in Oncology - SHATO , Sofia , Bulgaria.
authors.forename: Yordan
authors.initials: Y
authors.lastname: Simeonov
authors.affiliation: b National Reference Laboratory for Control and Monitoring of Antibiotic Resistance , Department of Microbiology, National Center of Infectious and Parasitic Diseases , Bulgaria.
authors.forename: Elina
authors.initials: E
authors.lastname: Dobreva
authors.affiliation: b National Reference Laboratory for Control and Monitoring of Antibiotic Resistance , Department of Microbiology, National Center of Infectious and Parasitic Diseases , Bulgaria.
authors.forename: Krasimira
authors.initials: K
authors.lastname: Ivanova
authors.affiliation: b National Reference Laboratory for Control and Monitoring of Antibiotic Resistance , Department of Microbiology, National Center of Infectious and Parasitic Diseases , Bulgaria.
authors.forename: Tzvetan
authors.initials: T
authors.lastname: Velinov
authors.affiliation: b National Reference Laboratory for Control and Monitoring of Antibiotic Resistance , Department of Microbiology, National Center of Infectious and Parasitic Diseases , Bulgaria.
authors.forename: Todor
authors.initials: T
authors.lastname: Kantardjiev
mesh_terms: D000328:Adult; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D002031:Bulgaria; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D005260:Female; D006801:Humans; D007710:Klebsiella Infections / Q000188:drug therapy / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D058885:Multilocus Sequence Typing; D010538:Peritonitis / Q000382:microbiology; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000150:complications; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases
keywords: Carbapenem resistance; Klebsiella pneumoniae; OXA-48; Oxacillinases