title: Overexpression of ZmMAPK1 enhances drought and heat stress in transgenic Arabidopsis thaliana.
abstract: Mitogen-activated protein kinase (MAPK) signal transduction cascades play a crucial role in the response to extracellular stimuli in eukaryotes. A number of MAPK family genes have been isolated in plants, but the maize MAPK genes have been little studied. Here, we studied the role of maize MAP kinase 1 (ZmMAPK1) using gene expression, protein subcellular localization, transformation in Arabidopsis, expression patterns of the stress-responsive genes and physiological parameter analysis. Our physiological parameter analysis suggested that over-expression ZmMAPK1 can increase proline content and decrease malondialdehyde content under drought, and prevent chlorophyll loss and the production of scavenger reactive oxygen species under heat stress. The resistance characteristics of the over-expression of ZmMAPK1 were associated with a significant increase in survival rate. These results suggest that ZmMAPK1 plays a positive role in response to drought and heat stress in Arabidopsis, and provide new insights into the mechanisms of action of MAPK in response to abiotic stress in plants.
authors.affiliation: Henan Agricultural University, Synergetic Innovation Center of Henan Grain Crops, 63 Nongye Road, Zhengzhou, 450002, People's Republic of China.
authors.forename: Liuji
authors.initials: L
authors.lastname: Wu
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Zu
authors.forename: Huimin
authors.initials: H
authors.lastname: Zhang
authors.forename: Liancheng
authors.initials: L
authors.lastname: Wu
authors.forename: Zhangying
authors.initials: Z
authors.lastname: Xi
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000064:Acclimatization / Q000235:genetics / Q000502:physiology; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000502:physiology*; D001483:Base Sequence; D002734:Chlorophyll / Q000378:metabolism; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D006358:Hot Temperature; D008315:Malondialdehyde / Q000378:metabolism; D019950:Mitogen-Activated Protein Kinase 1 / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011392:Proline / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013312:Stress, Physiological; D015854:Up-Regulation; D003313:Zea mays / Q000201:enzymology* / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D011994:Recombinant Proteins; D002734:Chlorophyll; D008315:Malondialdehyde; D011392:Proline; D019950:Mitogen-Activated Protein Kinase 1