title: Experience-dependent regulation of tissue-type plasminogen activator in the mouse barrel cortex.
abstract: It has been suggested that tissue-type plasminogen activator (tPA), a serine protease, plays a key role in regulating the extracellular matrix core proteins, thereby impacting the structural plasticity in the cerebral cortex. Much is known about its role in regulating plasticity in the visual cortex. However, its permissive role has not been demonstrated to generalize to other cerebral cortical areas. By utilizing a combination of immunofluorescent histochemistry and confocal microscopy, we demonstrate that endogenous tPA is indeed present in the somatosensory cortex, and its expression is experience-dependent. Chronic sensory deprivation induced by whisker trimming from birth for one month leads to increased tPA immunoreactivity in all layers of the barrel cortex. Furthermore, tPA immunoreactivity remains high even after sensation has been restored to the mystacial pad (by allowing whiskers to grow back to full length for one month). Our results suggest that tPA levels in the cerebral cortex are regulated by sensory experience, and play a key role in regulating structural remodeling in the cerebral cortex.
authors.affiliation: Neuropsychology Subprogram, Psychology, 365th 5th Avenue, New York, NY 10016, USA.
authors.forename: Chia-Chien
authors.initials: CC
authors.lastname: Chen
authors.affiliation: Neuropsychology Subprogram, Psychology, 365th 5th Avenue, New York, NY 10016, USA.
authors.forename: Philip
authors.initials: P
authors.lastname: Chu
authors.affiliation: Neuropsychology Subprogram, Psychology, 365th 5th Avenue, New York, NY 10016, USA; Biology-Neuroscience Subprogram, The Graduate Center, CUNY, 365th 5th Avenue, New York, NY 10016, USA; Department of Psychology, Queens College, CUNY, 65-30 Kissena Boulevard, Flushing, NY 11367, USA. Electronic address: joshua.brumberg@qc.cuny.edu.
authors.forename: Joshua C
authors.initials: JC
authors.lastname: Brumberg
mesh_terms: D000367:Age Factors; D000818:Animals; D005260:Female; D008297:Male; D051379:Mice; D012683:Sensory Deprivation; D013003:Somatosensory Cortex / Q000254:growth & development / Q000378:metabolism*; D010959:Tissue Plasminogen Activator / Q000378:metabolism*; D014738:Vibrissae / Q000502:physiology
chemical_list: D010959:Tissue Plasminogen Activator
keywords: Barrel cortex; Sensory experience; tPA