title: Molecular characterization of an IL-1β gene from the large yellow croaker (Larimichthys crocea) and its effect on fish defense against Vibrio alginolyticus infection.
abstract: Interleukin 1β (IL-1β), the first interleukin to be characterized, plays a key role in regulating the immune response. In this study, we determined the cDNA and genomic DNA sequences of the IL-1β gene from the large yellow croaker, Larimichthys crocea. Phylogenetic analysis indicated that the IL-1β (LcIL-1β) gene was most closely related to that of European seabass (Dicentrarchus labrax), sharing 67.8% amino acid identity. In healthy large yellow croaker, LcIL-1β transcription was detected in all tested tissues, with the highest level found in the head kidney. Upon Vibrio alginolyticus infection, LcIL-1β transcription in all tested tissues was significantly upregulated. Intraperitoneal injection of recombinant LcIL-1β (rLcIL-1β) improved the survival rate and reduced the tissue bacterial load after V. alginolyticus infection. In addition, rLcIL-1β induced monocytes/macrophages (MO/MΦ) chemotaxis and increased phagocytosis and bactericidal activity in vitro. These results suggest that LcIL-1β plays an important role in the large yellow croaker immune response against V. alginolyticus.
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo 315211, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo 315211, China.
authors.forename: Yu-Hong
authors.initials: YH
authors.lastname: Shi
authors.forename: Xue-Heng
authors.initials: XH
authors.lastname: Zhang
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo 315211, China.
authors.forename: Chang-Hong
authors.initials: CH
authors.lastname: Li
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo 315211, China.
authors.forename: Ming-Yun
authors.initials: MY
authors.lastname: Li
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo 315211, China;Donghai Sea Collaborative Innovation Center for Industrial Upgrading Mariculture, Ningbo University, Ningbo 315211, China. jchen1975@163.com.
authors.forename: Jiong
authors.initials: J
authors.lastname: Chen
mesh_terms: D000818:Animals; D001483:Base Sequence; D005393:Fish Diseases / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D029941:Fish Proteins / Q000235:genetics* / Q000276:immunology; D053583:Interleukin-1beta / Q000235:genetics* / Q000276:immunology*; D008969:Molecular Sequence Data; D010473:Perciformes / Q000145:classification / Q000235:genetics / Q000276:immunology; D010802:Phylogeny; D014735:Vibrio Infections / Q000235:genetics / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D044149:Vibrio alginolyticus / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*
chemical_list: D029941:Fish Proteins; D053583:Interleukin-1beta
keywords: Interleukin 1β; Large yellow croaker; Monocytes/ macrophages; Survival rate; Vibrio alginolyticus