title: Three-phase molecularly imprinted sol-gel based hollow fiber liquid-phase microextraction combined with liquid chromatography-tandem mass spectrometry for enrichment and selective determination of a tentative lung cancer biomarker.
abstract: In the present study, the modification of a polysulfone hollow fiber membrane with in situ molecularly imprinted sol-gel process (as a novel and one-step method) was prepared and investigated. 3-(propylmethacrylate)trimethoxysilane (3PMTMOS) as an inorganic precursor was used for preparation of molecularly imprinted sol-gel. The modified molecularly imprinted sol-gel hollow fiber membrane (MSHM) was used for the liquid-phase microextraction (LPME) of hippuric acid (HA) in human plasma and urine samples. MSHM as a selective, robust, and durable tool was used for at least 50 extractions without significant decrease in the extraction efficiency. The non-molecularly imprinted sol-gel hollow fiber membrane (NSHM) as blank hollow fiber membrane was prepared by the same process, only without HA. To achieve the best condition, influential parameters on the extraction efficiency were thoroughly investigated. The capability of this robust, green, and simple method for extraction of HA was successfully accomplished with LC/MS/MS. The limits of detection (LOD) and quantification (LOQ) in human plasma and urine samples were 0.3 and 1.0nmolL(-1), respectively. The standard calibration curves were obtained within the concentration range 1-2000nmolL(-1) for HA in human plasma and urine. The coefficients of determination (r(2)) were ≥0.998. The obtained data exhibited recoveries were higher than 89% for the extraction of HA in human plasma and urine samples.
authors.affiliation: Department of Chemistry, Amirkabir University of Technology, Hafez, Tehran, Iran.
authors.forename: Mohammad Mahdi
authors.initials: MM
authors.lastname: Moein
authors.affiliation: Department of Chemistry, Amirkabir University of Technology, Hafez, Tehran, Iran. Electronic address: mehranjavanbakht@gmail.com.
authors.forename: Mehran
authors.initials: M
authors.lastname: Javanbakht
authors.affiliation: Department of Textile Engineering, Amirkabir University of Technology, Hafez, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Karimi
authors.affiliation: Food and Drug Laboratory Research Center, Food and Drug Organization, Ministry of Health and Medical Education, Tehran, Iran.
authors.forename: Behrouz
authors.initials: B
authors.lastname: Akbari-Adergani
authors.affiliation: Department of Analytical Chemistry, Stockholm University, SE10691 Stockholm, Sweden; National Research Center, Cairo, Egypt.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Abdel-Rehim
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood* / Q000652:urine; D002853:Chromatography, Liquid / Q000379:methods; D004867:Equipment Design; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D016014:Linear Models; D059627:Liquid Phase Microextraction / Q000295:instrumentation / Q000379:methods*; D008175:Lung Neoplasms / Q000097:blood* / Q000652:urine; D008567:Membranes, Artificial; D054802:Molecular Imprinting / Q000295:instrumentation / Q000379:methods*; D044367:Phase Transition; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods; D013696:Temperature
chemical_list: D014408:Biomarkers, Tumor; D008567:Membranes, Artificial
keywords: Hippuric acid; Hollow fiber membrane; Liquid phase microextraction; Mass spectrometry; Molecularly imprinted polymers; Sol–gel