title: Co-extraction of DNA and PLFA from soil samples.
abstract: Lipid/DNA co-extraction from one sample is attractive in limiting biases associated with microbial community analysis from separate extractions. We sought to enhance established co-extraction methods and use high-throughput 16S rRNA sequencing to identify preferentially extracted taxa from co-extracted DNA. Co-extraction results in low DNA yields and distinct community structure changes.
authors.affiliation: Department of Microbiology, College of Arts & Sciences, University of Tennessee, Knoxville, TN 37996, United States.
authors.forename: Sheridan
authors.initials: S
authors.lastname: Brewer
authors.affiliation: Department of Civil & Environmental Engineering, College of Engineering, University of Tennessee, United States; Center for Environmental Biotechnology, University of Tennessee, United States.
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Techtmann
authors.affiliation: Department of Civil & Environmental Engineering, College of Engineering, University of Tennessee, United States; Center for Environmental Biotechnology, University of Tennessee, United States.
authors.forename: Nagissa
authors.initials: N
authors.lastname: Mahmoudi
authors.affiliation: Center for Environmental Biotechnology, University of Tennessee, United States; College of Arts and Sciences, Georgia State University, Atlanta, GA 30302, United States.
authors.forename: Dijibril
authors.initials: D
authors.lastname: Niang
authors.affiliation: Department of Microbiology, College of Arts & Sciences, University of Tennessee, Knoxville, TN 37996, United States; Center for Environmental Biotechnology, University of Tennessee, United States.
authors.forename: Susan
authors.initials: S
authors.lastname: Pfiffner
authors.affiliation: Department of Microbiology, College of Arts & Sciences, University of Tennessee, Knoxville, TN 37996, United States; Department of Civil & Environmental Engineering, College of Engineering, University of Tennessee, United States; Department of Earth & Planetary Sciences, College of Arts and Sciences, University of Tennessee, United States; Center for Environmental Biotechnology, University of Tennessee, United States. Electronic address: tchazen@utk.edu.
authors.forename: Terry C
authors.initials: TC
authors.lastname: Hazen
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000379:methods*; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D044822:Biodiversity; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D005227:Fatty Acids / Q000302:isolation & purification* / Q000378:metabolism; D010742:Phospholipid Ethers / Q000302:isolation & purification / Q000378:metabolism; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010742:Phospholipid Ethers
keywords: 16S rRNA sequencing; Co-extraction; DNA; PLFA; Soil