title: Increased erythrocytic osmotic fragility in anemic domestic shorthair and purebred cats.
abstract: Increased erythrocytic osmotic fragility and splenomegaly have been reported in anemic Abyssinian and Somali cats. Here we report on this condition in anemic domestic shorthair cats and two other breeds, and describe common features of the clinicopathological profiles, management and outcomes.
  
  
  
  Anemic cats, other than Abyssinians and Somalis, were included. The erythrocytic osmotic fragility test was performed, known causes of anemia were excluded, the illness was followed and medical records were reviewed.
  
  
  
  Twelve neutered cats were first found to be anemic between 0.5 and 9.0 years of age. Pallor, lethargy, inappetence, pica, weight loss and splenomegaly were commonly observed. A moderate-to-severe macrocytic and hypochromic anemia with variable regeneration was noted. Infectious disease screening, direct Coombs' and pyruvate kinase DNA mutation test results were negative. Freshly drawn blood did not appear hemolysed but became progressively lysed during storage at 4°C. The sigmoid osmotic fragility curves were moderately to severely right shifted, indicating erythrocytic fragility at 20°C. Cross-correction studies indicated an intrinsic red cell effect rather than plasma effect. Most cats were treated with immunosuppressive doses of prednisolone and doxycycline, with variable responses. Five cats with recurrent or persistent anemia responded well to splenectomy. However, two had occasional recurrence of severe anemia: one was found to be Bartonella vinsonii-positive during one episode and responded to azithromycin and prednisolone, while the other cat had two episodes of severe anemia of unknown cause. Finally, six cats were euthanized within 1 month and 7 years after initial presentation. Histopathology of six spleens revealed mainly congestion and extramedullary hematopoiesis.
  
  
  
  Similarly to Abyssinian and Somali cats, domestic shorthair and cats of other breeds can also develop severe erythrocytic osmotic fragility with anemia and splenomegaly, which should be considered as a differential diagnosis in anemic cats.
authors.affiliation: Section of Medical Genetics, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA.
authors.forename: Claudia
authors.initials: C
authors.lastname: Tritschler
authors.affiliation: Section of Medical Genetics, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA.
authors.forename: Keijiro
authors.initials: K
authors.lastname: Mizukami
authors.affiliation: Section of Medical Genetics, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA.
authors.forename: Karthik
authors.initials: K
authors.lastname: Raj
authors.affiliation: Section of Medical Genetics, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA giger@vet.upenn.edu.
authors.forename: Urs
authors.initials: U
authors.lastname: Giger
mesh_terms: D000740:Anemia / Q000097:blood / Q000473:pathology / Q000628:therapy / Q000662:veterinary*; D000818:Animals; D001774:Blood Chemical Analysis / Q000662:veterinary; D002371:Cat Diseases / Q000097:blood / Q000473:pathology* / Q000628:therapy; D002415:Cats; D003298:Coombs Test / Q000662:veterinary; D005260:Female; D008297:Male; D009996:Osmotic Fragility; D010375:Pedigree; D013163:Splenomegaly / Q000473:pathology / Q000662:veterinary