title: [Effect of irradiated human lung fibroblasts on activation of canonical Wnt/β-catenin signaling pathway in mesenchymal stem cells].
abstract: To investigate the effect of irradiated human lung fibroblasts (HLFs) on the canonical Wnt/β-catenin signaling pathway in human umbilical cord mesenchymal stem cells (HUMSCs).
  
  
  
  HUMSCs were cultured alone (single group) or co-cultured with HLFs exposed to 5Gy X-rays (co-culture group). The protein levels of GSK-3β, p-GSK-3β, FRAT1 and β-catenin in HUMSCs were examined by Western blotting 3 days after culture or co-culture. WISP-1 protein levels in conditioned medium were examined by ELISA.
  
  
  
  The levels of p-GSK3β/GSK3β (0.15 ± 0.05), FRAT1 (0.48 ± 0.07) and β-catenin (0.50 ± 0.07) in co-cultured HUMSCs significantly decreased compared to those in single group (0.55 ± 0.05, 1.16 ± 0.13 and 2.39 ± 0.15, all P<0.05). The supernatant level of WISP-1 in co-culture group was significantly decreased [(602.23 ± 161.47) ng/mL], compared to the single group [(977.77 ± 110.56) ng/mL, P<0.05].
  
  
  
  Irradiated HLFs attenuate the activation of canonical Wnt/β-catenin signaling pathway in HUMSCs in vitro.
authors.affiliation: 1.Graduate Student Team of Medical School of Chinese PLA, Beijing 100853, China;2.Department of Respiratory Medicine, Navy General Hospital, Beijing 100048, China;
authors.forename: Chun-yang
authors.initials: CY
authors.lastname: Zhang
authors.affiliation: Department of Respiratory Medicine, Navy General Hospital, Beijing 100048, China;
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Respiratory Medicine, Navy General Hospital, Beijing 100048, China;
authors.forename: Hua-song
authors.initials: HS
authors.lastname: Feng
authors.affiliation: Department of Respiratory Medicine, Navy General Hospital, Beijing 100048, China;
authors.forename: Xu-xin
authors.initials: XX
authors.lastname: Chen
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D055511:CCN Intercellular Signaling Proteins / Q000378:metabolism; D002478:Cells, Cultured; D018920:Coculture Techniques; D005347:Fibroblasts / Q000166:cytology* / Q000528:radiation effects; D005720:Gamma Rays; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism; D000071679:Glycogen Synthase Kinase 3 beta; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D014470:Umbilical Cord / Q000166:cytology; D060449:Wnt Signaling Pathway; D014965:X-Rays; D051176:beta Catenin / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D055511:CCN Intercellular Signaling Proteins; C116396:CCN4 protein, human; C495270:CTNNB1 protein, human; C491819:FRAT1 protein, human; D047908:Intracellular Signaling Peptides and Proteins; D011518:Proto-Oncogene Proteins; D051176:beta Catenin; C000605506:GSK3B protein, human; D000071679:Glycogen Synthase Kinase 3 beta; D038362:Glycogen Synthase Kinase 3