title: Development of Global Consensus of Dengue Virus Envelope Glycoprotein for Epitopes Based Vaccine Design.
abstract: Dengue virus (DENV) is the member of Flaviviridae and causative agent of Dengue Haemorrhagic Fever and Dengue Shock Syndrome. Every year, around 70% of the world population is at risk, due to epidemic episodes orchestrated by one or more of its serotypes. So, a tetravalent DENV vaccine is needed which may induce the immune response against all four DENV serotypes. In this study, B-cell and T-cell epitopes have been predicted from the DENV envelope glycoprotein (Eg) using a consensus based approach in complement with the physico-chemical property (PCP) conservancy analysis. Through DENV-Eg analysis, a total of 7 PCP conserved, water soluble, in vitro and in vivo stable epitopes were predicted which may induce the B-cell and T-cell mediated anti-viral immune response.
authors.affiliation: National Centre of Excellence in Molecular Biology, 87-West Canal Bank, Near Thokar Niaz Baig, Lahore. Pakistan. mazhar.hussain@cemb.edu.pk.
authors.forename: Mazhar
authors.initials: M
authors.lastname: Hussain
authors.forename: Muhammad
authors.initials: M
authors.lastname: Idrees
authors.forename: Samia
authors.initials: S
authors.lastname: Afzal
mesh_terms: D000595:Amino Acid Sequence; D003198:Computer Simulation; D017076:Computer-Aided Design; D032921:Consensus; D016384:Consensus Sequence; D017124:Conserved Sequence; D003715:Dengue / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D053059:Dengue Vaccines / Q000737:chemistry / Q000276:immunology*; D003716:Dengue Virus / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D015195:Drug Design; D018985:Epitopes, B-Lymphocyte / Q000737:chemistry / Q000276:immunology*; D018984:Epitopes, T-Lymphocyte / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*
chemical_list: D053059:Dengue Vaccines; D018985:Epitopes, B-Lymphocyte; D018984:Epitopes, T-Lymphocyte; D014759:Viral Envelope Proteins