title: Dealcoholized Korean Rice Wine (Makgeolli) Exerts Potent Anti-Tumor Effect in AGS Human Gastric Adenocarcinoma Cells and Tumor Xenograft Mice.
abstract: Makgeolli is a traditional wine in Korea and has been traditionally believed to exhibit health benefits. However, the inhibitory effect of dealcoholized makgeolli (MK) on cancer has never been investigated scientifically. In this study, MK exhibited an anti-angiogenic effect by inhibiting tube formation in human umbilical vein endothelial cells, without cytotoxicity. Treatment with MK reduced the proliferation of AGS human gastric adenocarcinoma cells in a dose-dependent manner and increased the sub-G1 population. Next, we evaluated whether MK could induce apoptosis in AGS cells by using the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay or Annexin V method. Treatment with MK at 500 and 1,000 μg/ml increased the number of TUNEL-positive AGS cells. Under the same conditions, MK-treated (500 and 1,000 μg/ml) cells showed significant induction of early or late apoptosis, compared with untreated cells (no induction). In addition, MK also induced phosphatase and tensin homolog (PTEN) expression in AGS cells. However, p53 expression in AGS cells was not changed by MK treatment. Furthermore, MK at 500 mg/kg·d reduced the tumor size and volume in AGS tumor xenografts. Taken together, MK may be useful for the prevention of cancer cell growth.
authors.affiliation: Korea Food Research Institute, Seongnam 463-746, Republic of Korea.|Department of Food Biotechnology, Korea University of Science and Technology, Daejeon 305-333, Republic of Korea.
authors.forename: Eun Ju
authors.initials: EJ
authors.lastname: Shin
authors.affiliation: Korea Food Research Institute, Seongnam 463-746, Republic of Korea.
authors.forename: Sung Hee
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Korea Food Research Institute, Seongnam 463-746, Republic of Korea.
authors.forename: Jae Ho
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Korea Food Research Institute, Seongnam 463-746, Republic of Korea.|Department of Food Biotechnology, Korea University of Science and Technology, Daejeon 305-333, Republic of Korea.
authors.forename: Jaeho
authors.initials: J
authors.lastname: Ha
authors.affiliation: Korea Food Research Institute, Seongnam 463-746, Republic of Korea.|Department of Food Biotechnology, Korea University of Science and Technology, Daejeon 305-333, Republic of Korea.
authors.forename: Jin-Taek
authors.initials: JT
authors.lastname: Hwang
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy*; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001688:Biological Products / Q000008:administration & dosage / Q000494:pharmacology*; D002460:Cell Line; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000187:drug effects* / Q000502:physiology; D004847:Epithelial Cells / Q000187:drug effects* / Q000502:physiology; D064593:Heterografts; D006801:Humans; D007723:Korea; D051379:Mice; D014920:Wine
chemical_list: D000970:Antineoplastic Agents; D001688:Biological Products
keywords: AGS human gastric adenocarcinoma cells; Makgeolli; Tumor xenograft; anti-angiogenesis; anti-cancer