title: Maximum parsimony based resolution of inter-species phylogenetic relationships inCitrusL. (Rutaceae) using ITS of rDNA.
abstract: The present study aims to analyse phylogenetic relationships, using internal transcribed spacer sequence data of ribosomal DNA (rDNA), across 24Citrusspecies and close relatives by the evaluation of several parameters such as nucleotide substitution (r), nucleotide diversity (π) and the estimated values of transition/transversion bias (R). The observed results indicated the presence of a wide divergence pattern of rDNA in subfamily Aurantioideae. Maximum parsimony (MP) analysis inferred divergence pattern in theCitrusgenus. We observed seven strongly supported clades among the subfamily Aurantioideae. We postulate that the present investigation provides a more robust topology ofCitrusand its close relatives, which can significantly prove as an additional support to resolve the phylogenetic relationships inCitrusgenera. Therefore, sequences of noncoding regions should exhibit more phylogenetically informative sites than the coding regions do, which is in accordance with the present study.
authors.affiliation: Huazhong Agricultural University, Key Laboratory of Horticultural Plant Biology (MOE) , Wuhan , China ; Desert Research Center, Egyptian Deserts Gene Bank , North Sinai , Egypt.
authors.forename: Mohamed Hamdy
authors.initials: MH
authors.lastname: Amar
authors.affiliation: Desert Research Center, Egyptian Deserts Gene Bank , North Sinai , Egypt.
authors.forename: Ahmed H M
authors.initials: AH
authors.lastname: Hassan
authors.affiliation: Huazhong Agricultural University, Key Laboratory of Horticultural Plant Biology (MOE) , Wuhan , China.
authors.forename: Manosh Kumar
authors.initials: MK
authors.lastname: Biswas
authors.affiliation: Bioversity International , Rome , Italy.
authors.forename: Ehsan
authors.initials: E
authors.lastname: Dulloo
authors.affiliation: Huazhong Agricultural University, Key Laboratory of Horticultural Plant Biology (MOE) , Wuhan , China.
authors.forename: Zong-Zhou
authors.initials: ZZ
authors.lastname: Xie
authors.affiliation: Huazhong Agricultural University, Key Laboratory of Horticultural Plant Biology (MOE) , Wuhan , China.
authors.forename: Wen-Wu
authors.initials: WW
authors.lastname: Guo
keywords: Aurantioideae; Citrus; ITS; Rutaceae; phylogenetic relationship; ribosomal DNA (rDNA)