title: Biogenesis, characterization, and the effect of vicenin-gold nanoparticles on glucose utilization in 3T3-L1 adipocytes: a bioinformatic approach to illuminate its interaction with PTP 1B and AMPK.
abstract: This study reported the synthesis of Vicenin-2 gold nanoparticles (VN-AuNPs) and evaluated their effect on the glucose utilization efficiency of 3T3-L1 adipocytes. The VN-AuNPs were characterized by microscopic, DLS and spectral analysis. The bio-reducing efficiency of Vicenin-2 (VN) was computed and confirmed by HPLC analysis. The stability of VN-AuNPs in various physiological media was explored. The cytotoxicity and glucose uptake assays were performed in 3T3-L1 adipocytes. The docking of VN with PTP1B and AMPK was also performed. The color change and UV absorption at 537 nm preliminarily confirmed the VN reduced gold nanoparticles. The VN-AuNPs appeared as spherical particles (57 nm) and face centered cubic crystals under TEM and XRD analysis, respectively. Its zeta potential was found to be -6.53 mV. The FT-IR spectra of VN and its AuNPs confirmed its stability. The computed reducing potential of VN was similar to the extent of VN utilized during the synthesis of VN-AuNPs. The VN-AuNPs showed a remarkable stability in different physiological media. At 100 µM concentration, VN-AuNPs displayed 78.21% cell viability. A concentration dependent increase in glucose uptake was noted in 3T3-L1 adipocytes when incubated with VN-AuNPs. The docking data revealed a strong interaction of VN with the binding pockets of PTP1B and AMPK. This demonstrates that the fabricated VN-AuNPs might enhance the intracellular VN availability mediated cellular glucose utilization and this would serve as a novel nanodrug for the management of diabetes.
authors.affiliation: Department of Biotechnology, Rajalakshmi Engineering College, Thandalam, Chennai, Tamil Nadu, 602 105, India.
authors.forename: Shivashri
authors.initials: S
authors.lastname: Chockalingam
authors.affiliation: Department of Biotechnology, Rajalakshmi Engineering College, Thandalam, Chennai, Tamil Nadu, 602 105, India.
authors.forename: Rajarajeshwari
authors.initials: R
authors.lastname: Thada
authors.affiliation: Department of Biotechnology, Rajalakshmi Engineering College, Thandalam, Chennai, Tamil Nadu, 602 105, India.
authors.forename: Ramesh Kumar
authors.initials: RK
authors.lastname: Dhandapani
authors.affiliation: Department of Biotechnology, Rajalakshmi Engineering College, Thandalam, Chennai, Tamil Nadu, 602 105, India.
authors.forename: Rajasekar
authors.initials: R
authors.lastname: Panchamoorthy
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D017667:Adipocytes; D000818:Animals; D047310:Apigenin / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D019295:Computational Biology; D005947:Glucose / Q000378:metabolism*; D005960:Glucosides / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D006046:Gold / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D051379:Mice; D062105:Molecular Docking Simulation; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1 / Q000378:metabolism*
chemical_list: D005960:Glucosides; C530449:apigenin-6,8-di-C-glycopyranoside; D006046:Gold; D047310:Apigenin; D055372:AMP-Activated Protein Kinases; C516607:PTPN1 protein, human; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1; D005947:Glucose
keywords: 3T3-L1 adipocytes; characterization; glucose uptake; gold nanoparticles; stability; vicenin-2