title: Molecular engineering of a dual emission near-infrared ratiometric fluorophore for the detection of pH at the organism level.
abstract: A near-infrared ratiometric fluorophore (NIR-HBT) was rationally designed and constructed by expanding both the excitation and emission wavelength of the classical ratiometric fluorophore 2-(benzothiazol-2-yl)phenol (HBT) into the near-infrared region. The NIR-HBT was easily synthesized by incorporating the HBT module into the hemicyanine skeleton and showed evident NIR ratiometric fluorophore characteristics. Further application of the new fluorophore for pH detection demonstrated that NIR-HBT possesses superior overall analytical performance and NIR-HBT was successfully applied for detection of acidosis caused by inflammation in living animal tissue, which indicated the potential application value of NIR-HBT in biological imaging and sensing.
authors.affiliation: Key Laboratory of Green Chemistry and Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu, 610064, P. R. China. xqyu@scu.edu.cn.
authors.forename: Bo-Lin
authors.initials: BL
authors.lastname: Wang
authors.forename: Chuang
authors.initials: C
authors.lastname: Jiang
authors.forename: Kun
authors.initials: K
authors.lastname: Li
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Liu
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Xie
authors.forename: Xiao-Qi
authors.initials: XQ
authors.lastname: Yu
mesh_terms: D000818:Animals; D052160:Benzothiazoles / Q000737:chemistry; D015195:Drug Design; D005456:Fluorescent Dyes / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008168:Lung / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D057054:Molecular Imaging; D019265:Spectroscopy, Near-Infrared / Q000379:methods*
chemical_list: C587232:2-(benzothiazol-2-yl)-phenol; D052160:Benzothiazoles; D005456:Fluorescent Dyes