title: [Detection of West Nile virus RNA and specific antibodies in blood donors of Volgograd region].
abstract: OBJECTIVE
  Study infection level by West Nile virus (WNV) of donors living on the territory of West Nile fever (WNF) foci with long-term activity, and evaluate the possibility of non-transmissible transfer of the causative agent during use of donor blood.
  
  
  METHODS
  Blood sera (432) collected in August and September 2012 in Volgograd and Volzhsky cities were studied by PCR and EIA.
  
  
  RESULTS
  Antibodies to WNV were detected in 16.2% (70/432); 8.8% (38/432) donors had IgG; 0.93% (4/432)--IgM and 6.5% (28/432)--IgG and IgM antibodies simultaneously. WNV RNA was detected in one of the samples in the last group. Thus, 5 donors (1.1%, 5/432) were at the early stages after infection.
  
  
  CONCLUSIONS
  The data obtained give evidence on the necessity to execute control of donor blood by PCR and EIA in WNF foci with long-term activity.
authors.forename: L S
authors.initials: LS
authors.lastname: Karan
authors.forename: M V
authors.initials: MV
authors.lastname: Fedorova
authors.forename: K A
authors.initials: KA
authors.lastname: Gridneva
authors.forename: A N
authors.initials: AN
authors.lastname: Chaika
authors.forename: E I
authors.initials: EI
authors.lastname: Romasova
mesh_terms: D000914:Antibodies, Viral / Q000097:blood*; D001782:Blood Donors; D062665:Epidemiological Monitoring; D005490:Focal Infection; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D012367:RNA, Viral / Q000097:blood*; D012426:Russia / Q000453:epidemiology; D014901:West Nile Fever / Q000453:epidemiology* / Q000276:immunology / Q000635:transmission / Q000821:virology; D014902:West Nile virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012367:RNA, Viral