title: Studies on the antioxidant properties of extracts from the roots and shoots of two Scutellaria species in human blood plasma.
abstract: We determined the in vitro antioxidant activity of methanolic extracts from the shoots and roots of Scutellaria species (S. altissima and S. alpina) against the action of strong oxidants: hydrogen peroxide (H2O2) and H2O2+Fe(2+) (donor of hydroxyl radicals) on plasma proteins and lipids. Lipid peroxidation in human plasma was measured by the level of thiobarbituric acid reactive species (TBARS). Protein oxidation was measured by quantitation of thiol group. We observed that the extracts (5-50 µg ml(-1)) containing phenolic compounds from both Scutellaria species distinctly reduced oxidation of lipids and proteins in human plasma treated with H2O2. These results also indicated that the extracts have a protective effect against oxidative damage to the human plasma lipids and proteins by induced hydroxyl radical. The main components of the plant materials analysed were flavonoids, present as aglycones (luteolin) or glycosides (cynaroside, baicalin, wogonoside). In all of the extracts, the phenylethanoid verbascoside was also found. The properties of the tested plant extracts were also compared with the action of a well characterised commercial antioxidative polyphenolic extract from the berries of Aronia melanocarpa (Aronox(®)). The comparative studies indicated that the analysed plant extracts were comparable to or even more effective in reducing the oxidation processes than the A. melanocarpa extract. The present study suggests that natural extracts from S. altissima and S. alpina have antioxidant activities and, therefore, may be beneficial in the prevention of diseases related to oxidant stress, such as cancer, cardiovascular, and inflammatory diseases.
authors.affiliation: Department of Biology and Pharmaceutical Botany, Medical University, Łódź, Poland.
authors.forename: Izabela
authors.initials: I
authors.lastname: Grzegorczyk-Karolak
authors.affiliation: Department of Biology and Pharmaceutical Botany, Medical University, Łódź, Poland.
authors.forename: Halina
authors.initials: H
authors.lastname: Wysokińska
authors.affiliation: Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Łódź, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Olas
mesh_terms: D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology*; D001769:Blood / Q000187:drug effects; D044950:Flavanones / Q000032:analysis; D005419:Flavonoids / Q000032:analysis; D005960:Glucosides / Q000032:analysis / Q000494:pharmacology; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D015394:Molecular Structure; D018384:Oxidative Stress / Q000187:drug effects; D010636:Phenols / Q000032:analysis / Q000494:pharmacology; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D018520:Plant Shoots / Q000737:chemistry; D031363:Scutellaria / Q000737:chemistry*; D059808:Polyphenols
chemical_list: D000975:Antioxidants; D044950:Flavanones; D005419:Flavonoids; D005960:Glucosides; D006861:Hydrogen Peroxide; D010636:Phenols; D010936:Plant Extracts; C038044:baicalin; C058956:acteoside; D059808:Polyphenols; C473995:wogonoside