title: Low Fouling Electrospun Scaffolds with Clicked Bioactive Peptides for Specific Cell Attachment.
abstract: While electrospun fibers are of interest as scaffolds for tissue engineering applications, nonspecific surface interactions such as protein adsorption often prevent researchers from controlling the exact interactions between cells and the underlying material. In this study we prepared electrospun fibers from a polystyrene-based macroinitiator, which were then grafted with polymer brushes using surface-initiated atom transfer radical polymerization (SI-ATRP). These brush coatings incorporated a trimethylsilyl-protected PEG-alkyne monomer, allowing azide functional molecules to be covalently attached, while simultaneously reducing nonspecific protein adsorption on the fibers. Cells were able to attach and spread on fibrous substrates functionalized with a pendant RGD-containing peptide, while spreading was significantly reduced on nonfunctionalized fibers and those with the equivalent RGE control peptide. This effect was observed both in the presence and absence of serum in the culture media, indicating that protein adsorption on the fibers was minimal and cell adhesion within the fibrous scaffold was mediated almost entirely through the cell-adhesive RGD-containing peptide.
authors.affiliation: ‡CSIRO Manufacturing Flagship, Bayview Avenue, Clayton 3168, Victoria, Australia.|§Cooperative Research Centre for Polymers, 8 Redwood Drive, Notting Hill 3168, Victoria, Australia.
authors.forename: Andrew E
authors.initials: AE
authors.lastname: Rodda
authors.affiliation: ∥ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Faculty of Pharmacy and Pharmaceutical Sciences, Monash University, 381 Royal Parade, Parkville 3052, Victoria, Australia.
authors.forename: Francesca
authors.initials: F
authors.lastname: Ercole
authors.affiliation: ‡CSIRO Manufacturing Flagship, Bayview Avenue, Clayton 3168, Victoria, Australia.
authors.forename: Veronica
authors.initials: V
authors.lastname: Glattauer
authors.affiliation: ‡CSIRO Manufacturing Flagship, Bayview Avenue, Clayton 3168, Victoria, Australia.
authors.forename: James
authors.initials: J
authors.lastname: Gardiner
authors.affiliation: ⊥School of Engineering, The Australian National University, Canberra 0200, Australian Capital Territory, Australia.
authors.forename: David R
authors.initials: DR
authors.lastname: Nisbet
authors.affiliation: #Departments of Bioengineering and Materials Science and Engineering, University of California at Berkeley, Berkeley, California, United States.
authors.forename: Kevin E
authors.initials: KE
authors.lastname: Healy
authors.forename: John S
authors.initials: JS
authors.lastname: Forsythe
authors.affiliation: ‡CSIRO Manufacturing Flagship, Bayview Avenue, Clayton 3168, Victoria, Australia.|§Cooperative Research Centre for Polymers, 8 Redwood Drive, Notting Hill 3168, Victoria, Australia.
authors.forename: Laurence
authors.initials: L
authors.lastname: Meagher
mesh_terms: D000327:Adsorption; D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology*; D008422:Materials Testing; D051379:Mice; D011137:Polystyrenes / Q000737:chemistry*; D013499:Surface Properties; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D011137:Polystyrenes