title: Vybrant DyeCycle Violet Stain Discriminates Two Different Subsets of CD34+ Cells.
abstract: BACKGROUND
  Studies are needed to understand the role of CD34 expressing cells with regard to efficient engraftment, especially in the adjuvant treatment of cancer.
  
  
  METHODS
  In this study we have used a modified method in our laboratory for routinely counting CD34+ cells. Unlysed whole blood samples were stained with the DNA-selective and cell membrane-permeant Vibrant DyeCycle Violet stain.
  
  
  RESULTS
  CD34+ cells exhibit a consistent and differential Vybrant Dye Cycle Violet staining pattern. Based on their different DCV intensity, we classified these subpopulations as CD34+/DCV(high) and CD34+/DCV(low) cells. In general, DCV(high) cells are about 12-times brighter than DCV(low) cells.
  
  
  CONCLUSIONS
  DCV staining may be used to discriminate subsets of CD34+ cells similarly to other methods which have previously defined different functional properties that can be related to the characterization, resolution, and purification of primitive hematopoietic stem cells in combination with specific useful markers for multicolor flow cytometric measurements.
authors.forename: Cristian
authors.initials: C
authors.lastname: Núñez-Espinosa
authors.forename: María D
authors.initials: MD
authors.lastname: García-Godoy
authors.forename: Inês
authors.initials: I
authors.lastname: Ferreira
authors.forename: Juan G
authors.initials: JG
authors.lastname: Ríos-Kristjánsson
authors.forename: David
authors.initials: D
authors.lastname: Rizo-Roca
authors.forename: Laura G
authors.initials: LG
authors.lastname: Rico
authors.forename: Gerard
authors.initials: G
authors.lastname: Rubí-Sans
authors.forename: Carlos
authors.initials: C
authors.lastname: Palacio
authors.forename: Joan R
authors.initials: JR
authors.lastname: Torrella
authors.forename: Teresa
authors.initials: T
authors.lastname: Pagès
authors.forename: Mike D
authors.initials: MD
authors.lastname: Ward
authors.forename: Ginés
authors.initials: G
authors.lastname: Viscor
authors.affiliation: Josep Carreras Leukemia Research Institute, Crta. de Can Ruti, Camí de les Escoles s/n. Edifici IMPPC, 08916 Badalona (Barcelona, Spain. jpetriz@carrerasresearch.org.
authors.forename: Jordi
authors.initials: J
authors.lastname: Petriz
mesh_terms: D000818:Animals; D018952:Antigens, CD34; D001562:Benzimidazoles; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes; D006412:Hematopoietic Stem Cells / Q000145:classification*; D006801:Humans; D008297:Male; D051381:Rats
chemical_list: D018952:Antigens, CD34; D001562:Benzimidazoles; C580833:DyeCycle Violet; D005456:Fluorescent Dyes