title: Chemical and biological evaluation of Egyptian Nile Tilapia (Oreochromis niloticas) fish scale collagen.
abstract: Collagen is considered to be one of the most useful biomaterials with different medical applications. However, collagen properties differ from one source to another. The aim of this study was to extract, purify, characterize and perform preliminary biological evaluation of type I collagen from scales of Egyptian Nile Tilapia. Pepsin-solubilized collagen (PSC) was successfully prepared from Nile Tilapia fish scale waste. Lyophilized collagen was dissolved in dilute HCl to form acidic collagen solutions (ACS) which was neutralized to form gel. To confirm the biocompatibility of the produced gel, baby hamster kidney (BHK-21) fibroblast cells were seeded onto a 3D collagen gel (0.3% and 0.5%, w/v). The results of an SDS-PAGE test showed that the extracted collagens were type I collagen, with α chain composition of (α1)2α2. Thermal analysis showed that the denaturation temperature was 32 °C. X-ray diffraction (XRD) analysis and Fourier-transform infrared spectra (FTIR) showed that the extracted collagen had a triple helix structure. Active proliferation of BHK-21 cells with no signs of toxicity was evident with both collagen gel concentrations tested. The results show that Nile Tilapia scales can be an effective source of collagen extraction that could be used as a potential biomaterial in biomedical applications.
authors.affiliation: Department of Biomaterials, Faculty of Oral and Dental Medicine, Cairo University, 11562 Cairo, Egypt. Electronic address: aiah.abdelwahab@dentistry.cu.edu.eg.
authors.forename: Aiah A
authors.initials: AA
authors.lastname: El-Rashidy
authors.affiliation: Department of Animal Production, Faculty of Agriculture, Cairo University, 12613 Giza, Egypt; Cairo University Research Park (CURP), Faculty of Agriculture, Cairo University, 12613 Giza, Egypt.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Gad
authors.affiliation: Cairo University Research Park (CURP), Faculty of Agriculture, Cairo University, 12613 Giza, Egypt.
authors.forename: Abd El-Hay G
authors.initials: Ael-H
authors.lastname: Abu-Hussein
authors.affiliation: Department of Biomaterials, Faculty of Oral and Dental Medicine, Cairo University, 11562 Cairo, Egypt.
authors.forename: Shaymaa I
authors.initials: SI
authors.lastname: Habib
authors.affiliation: Department of Biomaterials, Faculty of Oral and Dental Medicine, Cairo University-Affiliated to Faculty of dentistry, Umm Al-Qura University, Saudi Arabia.
authors.forename: Nadia A
authors.initials: NA
authors.lastname: Badr
authors.affiliation: Department of Biomaterials, Faculty of Oral and Dental Medicine, Cairo University, 11562 Cairo, Egypt.
authors.forename: Azza A
authors.initials: AA
authors.lastname: Hashem
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion; D002460:Cell Line; D048430:Cell Shape / Q000187:drug effects; D023681:Cichlids; D024042:Collagen Type I / Q000737:chemistry* / Q000494:pharmacology / Q000648:ultrastructure; D006224:Cricetinae; D003470:Culture Media; D029941:Fish Proteins / Q000737:chemistry* / Q000494:pharmacology / Q000648:ultrastructure; D005782:Gels / Q000737:chemistry / Q000494:pharmacology; D008422:Materials Testing; D011489:Protein Denaturation; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D024042:Collagen Type I; D003470:Culture Media; D029941:Fish Proteins; D005782:Gels
keywords: BHK-21 (Baby Hamster kidney); Characterization; Fish scale; Nile Tilapia; Type I collagen