title: Calcium Imaging in Pluripotent Stem Cell-Derived Cardiac Myocytes.
abstract: The possibility to generate cardiomyocytes (CMs) from disease-specific induced pluripotent stem cells (iPSCs) is a powerful tool for the investigation of various cardiac diseases in vitro. The pathological course of various cardiac conditions, causatively heterogeneous, often converges into disturbed cellular Ca(2+) cycling. The gigantic Ca(2+) channel of the intracellular Ca(2+) store of CMs, the ryanodine receptor type 2 (RyR2), controls Ca(2+) release and therefore plays a crucial role in Ca(2+) cycling of CMs. In the present protocol we describe ways to measure and analyze global as well as local cellular Ca(2+) release events in CMs derived from a patient carrying a CPVT-causing RyR2 mutation.
authors.affiliation: Center for Physiology and Pathophysiology, Institute for Vegetative Physiology, Medical Faculty, University of Cologne, Robert-Koch-Str. 39, 50931, Cologne, Germany.
authors.forename: Anna
authors.initials: A
authors.lastname: Walter
authors.affiliation: Center for Physiology and Pathophysiology, Institute for Neurophysiology, Medical Faculty, University of Cologne, Robert-Koch-Str. 39, 50931, Cologne, Germany. tomo.saric@uni-koeln.de.
authors.forename: Tomo
authors.initials: T
authors.lastname: Šarić
authors.affiliation: Center for Physiology and Pathophysiology, Institute for Neurophysiology, Medical Faculty, University of Cologne, Robert-Koch-Str. 39, 50931, Cologne, Germany.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Hescheler
authors.affiliation: Center for Physiology and Pathophysiology, Institute for Vegetative Physiology, Medical Faculty, University of Cologne, Robert-Koch-Str. 39, 50931, Cologne, Germany. symeon.papadopoulos@uk-koeln.de.
authors.forename: Symeon
authors.initials: S
authors.lastname: Papadopoulos
mesh_terms: D000814:Aniline Compounds; D000818:Animals; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D065150:Cellular Reprogramming; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D007545:Isoproterenol / Q000494:pharmacology; D051379:Mice; D057054:Molecular Imaging / Q000379:methods; D009154:Mutation; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D061251:Primary Cell Culture; D012433:Ryanodine / Q000494:pharmacology; D019837:Ryanodine Receptor Calcium Release Channel / Q000235:genetics / Q000378:metabolism; D013610:Tachycardia / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D014966:Xanthenes
chemical_list: D000814:Aniline Compounds; C409648:Fluo 4; D019837:Ryanodine Receptor Calcium Release Channel; D014966:Xanthenes; D012433:Ryanodine; D007545:Isoproterenol; D002118:Calcium
keywords: Calcium transients; Cardiomyocytes; Confocal microscopy; Sparks; Tachycardia