title: Utilization of Bacillus sp. strain TAT105 as a biological additive to reduce ammonia emissions during composting of swine feces.
abstract: Bacillus sp. strain TAT105 is a thermophilic, ammonium-tolerant bacterium that grows assimilating ammonium nitrogen and reduces ammonia emission during composting of swine feces. To develop a practical use of TAT105, a dried solid culture of TAT105 (5.3 × 10(9) CFU/g of dry matter) was prepared as an additive. It could be stored for one year without significant reduction of TAT105. Laboratory-scale composting of swine feces was conducted by mixing the additive. When the additive, mixed with an equal weight of water one day before use, was added to obtain a TAT105 concentration of above 10(7) CFU/g of dry matter in the initial material, the ammonia concentration emitted was lower and nitrogen loss was approximately 22% lower in the treatment with the additive than in the control treatment without the additive. The colony formation on an agar medium containing high ammonium could be used for enumeration of TAT105 in the composted materials.
authors.affiliation: a Livestock and Grassland Research Division , NARO Kyushu Okinawa Agricultural Research Center, National Agriculture and Food Research Organization (NARO/KARC) , Kohshi , Japan.
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Kuroda
authors.forename: Miyoko
authors.initials: M
authors.lastname: Waki
authors.forename: Tomoko
authors.initials: T
authors.lastname: Yasuda
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Fukumoto
authors.forename: Akihiro
authors.initials: A
authors.lastname: Tanaka
authors.forename: Kiyohiko
authors.initials: K
authors.lastname: Nakasaki
mesh_terms: D000393:Air Pollutants / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D001407:Bacillus / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D015373:Bacterial Typing Techniques; D001673:Biodegradation, Environmental; D005243:Feces / Q000382:microbiology*; D005285:Fermentation / Q000502:physiology; D008372:Manure / Q000382:microbiology*; D009584:Nitrogen / Q000032:analysis / Q000378:metabolism*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013552:Swine
chemical_list: D000393:Air Pollutants; D008372:Manure; D012336:RNA, Ribosomal, 16S; D009584:Nitrogen
keywords: ammonia emissions; biological additive; composting; swine feces; thermophilic ammonium-tolerant bacterium