title: Molecular cloning and expression studies of the adapter molecule myeloid differentiation factor 88 (MyD88) in turbot (Scophthalmus maximus).
abstract: Myeloid differentiation factor 88 (MyD88) is an adapter protein involved in the interleukin-1 receptor (IL-1R) and Toll-like receptor (TLR)-mediated activation of nuclear factor-kappaB (NF-κB). In this study, a full length cDNA of MyD88 was cloned from turbot, Scophthalmus maximus. It is 1619 bp in length and contains an 858-bp open reading frame that encodes a peptide of 285 amino acid residues. The putative turbot (Sm)MyD88 protein possesses a N-terminal death domain and a C-terminal Toll/IL-1 receptor (TIR) domain known to be important for the functions of MyD88 in mammals. Phylogenetic analysis grouped SmMyD88 with other fish MyD88s. SmMyD88 mRNA was ubiquitously expressed in all examined tissues of healthy turbots, with higher levels observed in immune-relevant organs. To explore the role of SmMyD88, its gene expression profile in response to stimulation of lipopolysaccharide (LPS), CpG oligodeoxynucleotide (CpG-ODN) or turbot reddish body iridovirus (TRBIV) was studied in the head kidney, spleen, gills and muscle over a 7-day time course. The results showed an up-regulation of SmMyD88 transcript levels by the three immunostimulants in all four examined tissues, with the induction by CpG-ODN strongest and initiated earliest and inducibility in the muscle very weak. Additionally, TRBIV challenge resulted in a quite high level of SmMyD88 expression in the spleen, whereas the two synthetic immunostimulants induced the higher levels in the head kidney. These data provide insights into the roles of SmMyD88 in the TLR/IL-1R signaling pathway of the innate immune system in turbot.
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
authors.forename: Jing-Yun
authors.initials: JY
authors.lastname: Lin
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China; Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China. Electronic address: huguobin@mail.ouc.edu.cn.
authors.forename: Guo-Bin
authors.initials: GB
authors.lastname: Hu
authors.affiliation: College of Medicine, Qingdao University, Qingdao 266071, China.
authors.forename: Chang-Hong
authors.initials: CH
authors.lastname: Yu
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
authors.forename: Song
authors.initials: S
authors.lastname: Li
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
authors.forename: Qiu-Ming
authors.initials: QM
authors.lastname: Liu
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China; Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China.
authors.forename: Shi-Cui
authors.initials: SC
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism*; D005412:Flatfishes / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D015870:Gene Expression; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000276:immunology; D005880:Gills / Q000276:immunology / Q000378:metabolism; D060226:Head Kidney / Q000276:immunology / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008969:Molecular Sequence Data; D053594:Myeloid Differentiation Factor 88 / Q000235:genetics / Q000378:metabolism*; D009928:Organ Specificity; D010802:Phylogeny; D013154:Spleen / Q000276:immunology / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D029941:Fish Proteins; D008070:Lipopolysaccharides; D053594:Myeloid Differentiation Factor 88
keywords: CpG-ODN; Gene expression; LPS; MyD88; Scophthalmus maximus; TRBIV