title: Dipeptides Increase Functional Activity of Human Skin Fibroblasts.
abstract: We analyzed the effect of dipeptide Glu-Trp and isovaleroyl-Glu-Trp in concentrations of 0.2, 2 and 20 μg/ml and Actovegin preparation on functional activity of human skin fibroblasts. Dipeptides, especially Glu-Trp, produce a stimulating effect on human skin fibroblasts and their effect is equivalent to that of Actovegin. Dipeptides stimulate cell renewal processes by activating synthesis of Ki-67 and reducing expression of caspase-9 and enhance antioxidant function of the cells by stimulating the expression of Hsp-90 and inducible NO-synthase. These findings suggest that dipeptides are promising candidates for preparations stimulating reparative processes.
authors.affiliation: Cytomed Medico-Biological Research-and-Production Complex, St. Petersburg, Russia, Vvm-61@mail.ru.
authors.forename: V V
authors.initials: VV
authors.lastname: Malinin
authors.forename: A O
authors.initials: AO
authors.lastname: Durnova
authors.forename: V O
authors.initials: VO
authors.lastname: Polyakova
authors.forename: I M
authors.initials: IM
authors.lastname: Kvetnoi
mesh_terms: D000975:Antioxidants / Q000502:physiology; D053453:Caspase 9 / Q000096:biosynthesis; D049109:Cell Proliferation / Q000187:drug effects; D004151:Dipeptides / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D018841:HSP90 Heat-Shock Proteins / Q000096:biosynthesis; D006418:Heme / Q000031:analogs & derivatives* / Q000494:pharmacology; D045782:Hemiterpenes; D006801:Humans; D019394:Ki-67 Antigen / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis; D010421:Pentanoic Acids / Q000494:pharmacology*; D012867:Skin / Q000166:cytology / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D004151:Dipeptides; D018841:HSP90 Heat-Shock Proteins; D045782:Hemiterpenes; D019394:Ki-67 Antigen; D010421:Pentanoic Acids; C008216:isovaleric acid; C057081:alpha-glutamyltryptophan; D006418:Heme; C015646:Actovegin; D052247:Nitric Oxide Synthase Type II; D053453:Caspase 9