title: [Effects of tamoxifen on CD147 glycosylation and MMPs in the diabetic rat myocardium].
abstract: OBJECTIVE
  Over the last few decades, diabetic cardiomyopathy has been identified as a significant contributor in cardiac morbidity. However, the mechanisms of diabetic cardiomyopathy have not been clarified.
  
  
  METHODS
  In the present study, a diabetic rat model was induced by the intraperitoneal injection of streptozotocin. The myocardial CD147 expression and extent of glycosylation, as well as thematrixmetalloproteinases(MMPs) expression and activity, were observed in the diabetic and synchronous rats.
  
  
  RESULTS
  The results showed that CD147 located on sarcolemma of cardiomyocytes. The myocardial CD147 expression and glycosylation were significantly increased in the diabetic rats as compared with the control. Expression of MMP-2 protein, MMP-2 and MMP-9 activity were also increased in left ventricular myocardium in the diabetic rats. Tamoxifen only inhibited the enhanced expression of myocardial CD147 in the diabetic rats, but not in synchronous control rats. Tamoxifen inhibited glycosylation of myocardial CD147 in both diabetic and control rats. The inhibition of tamoxifen on CD147 glycosylation was stronger than on the expression in the myocardium. The extent of myocardial CD147glycosylation was positively related toMMP-2 and MMP-9 activity. Tamoxifen induced an inhibition of myocardial MMP-2 and MMP-9 activity in the control and diabetic rats.
  
  
  CONCLUSIONS
  These results indicate that myocardial CD147 expression, especially the extent of glycosylation, regulates MMP-2 and MMP-9 activity, then accelerates cardiac pathological remodeling inducing diabetic cardiomyopathy. Tamoxifen inhibits myocardial CD147 glycosylation and further depress the activity of MMPs. Therefore, tamoxifen may protect the diabetic rats against diabetic myocardium.
authors.forename: Yi-xuan
authors.initials: YX
authors.lastname: Wang
authors.forename: Yun-tao
authors.initials: YT
authors.lastname: Gao
authors.forename: Long-biao
authors.initials: LB
authors.lastname: Cui
authors.forename: Ning-yu
authors.initials: NY
authors.lastname: Ru
authors.forename: Hai-jun
authors.initials: HJ
authors.lastname: Zhang
authors.forename: Bo
authors.initials: B
authors.lastname: Jiao
authors.forename: Zhi-bin
authors.initials: ZB
authors.lastname: Yu
mesh_terms: D000818:Animals; D051926:Basigin / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000150:complications; D058065:Diabetic Cardiomyopathies / Q000188:drug therapy*; D006031:Glycosylation; D006321:Heart / Q000187:drug effects; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000166:cytology; D051381:Rats; D012508:Sarcolemma / Q000378:metabolism; D013629:Tamoxifen / Q000494:pharmacology*
chemical_list: D013629:Tamoxifen; D051926:Basigin; D020778:Matrix Metalloproteinase 2; C522363:Mmp2 protein, rat; D020780:Matrix Metalloproteinase 9; C572337:Mmp9 protein, rat