title: [Intervention and therapeutic effect of siRNA-HDAC5 on abnormal histone modification in non-obese diabetic mice].
abstract: OBJECTIVE
  To evaluate therapeutic eff ect of siRNA-HDAC5 on non-obese diabetic (NOD) mice by using small interference RNA (siRNA) technique to knock down the expression of HDAC5 in spleen CD4+ T cells.
  
  
  METHODS
  NOD mice, 12-weeks old, were randomly divided into 3 groups and were given normal saline, siRNA-Control or siRNA-HDAC5 through caudal vein injection. The spleens and other samples were collected at the 18th, 24th or 30th week. The blood glucose was tested by blood glucose meter. The urinary albumin and serum levels of IL-1, IL-6, IL-18, and TNF-α were detected by ELISA. The mRNA levels of CD11a, CCR5, and CX3CR1 in spleen CD4+ T cells were measured by quantitative Real-time PCR. The HDAC5 protein level in spleen CD4+ T cell was detected by Western blot.
  
  
  RESULTS
  Compared with the control group, the siRNA-HDAC5 group showed a significant decrease in blood glucose, urine albumin excretion rate, serum cytokine and the mRNA levels of CD11a, CCR5, and CX3CR1, consist with the decrease in protein level of HDAC5.
  
  
  CONCLUSIONS
  Inhibition of HDAC5 expression in NOD mice could effectively alleviate the onset and development of kidney damage caused by diabetes.
authors.affiliation: Department of Intensive Care Unit, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Ouyang
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Wang
authors.forename: Lingjiao
authors.initials: L
authors.lastname: Liu
authors.forename: Youming
authors.initials: Y
authors.lastname: Peng
authors.forename: Can
authors.initials: C
authors.lastname: Hou
mesh_terms: D000818:Animals; D040881:CD11a Antigen / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism; D000073883:CX3C Chemokine Receptor 1; D016207:Cytokines / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000235:genetics / Q000628:therapy*; D004797:Enzyme-Linked Immunosorbent Assay; D042421:Histone Code; D006655:Histone Deacetylases / Q000235:genetics*; D051379:Mice; D016688:Mice, Inbred NOD; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000627:therapeutic use*; D011897:Random Allocation; D060888:Real-Time Polymerase Chain Reaction; D019713:Receptors, CCR5 / Q000378:metabolism; D019707:Receptors, Chemokine / Q000378:metabolism; D013154:Spleen / Q000166:cytology
chemical_list: C000592874:CCR5 protein, mouse; D040881:CD11a Antigen; D000073883:CX3C Chemokine Receptor 1; C471885:Cx3cr1 protein, mouse; D016207:Cytokines; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D019713:Receptors, CCR5; D019707:Receptors, Chemokine; C498618:Hdac5 protein, mouse; D006655:Histone Deacetylases