title: Oxidized-low density lipoprotein accumulates cholesterol esters via the PKCα-adipophilin-ACAT1 pathway in RAW264.7 cells.
abstract: Oxidized low‑density lipoprotein (ox‑LDL) can increase the expression of adipophilin and the accumulation of intracellular lipid droplets. However, the detailed mechanisms remain to be fully elucidated. The present study aimed to investigate the mechanism underlying the effect of ox‑LDL on the expression of adipophilin and the accumulation of intracellular cholesterol esters. The results revealed that ox‑LDL increased the activation of protein kinase C α (PKCα), expression of adipophilin and acyl‑coenzymeA: cholesterol acyltransferse 1 (ACAT1) and increased accumulation of intracellular cholesterol esters. In addition, PKCα siRNA abrogated ox‑LDL‑induced adipophilin, expression of ATAC1 and accumulation of cholesterol esters. Furthermore, ox‑LDL increased the accumulation of intracellular cholesterol esters and expression of ACAT1, and this effect were reversed by transfection with adipophilin siRNA. Taken together, these results demonstrated that ox‑LDL induces the accumulation of cholesterol esters, which is mediated by the PKCα‑adipophilin‑ACAT1 pathway.
authors.affiliation: Institute of Cardiovascular Disease, Key Laboratory for Arteriosclerology of Hunan, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Yuncheng
authors.initials: Y
authors.lastname: Qiao
authors.affiliation: Institute of Cardiovascular Disease, Key Laboratory for Arteriosclerology of Hunan, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Dongming
authors.initials: D
authors.lastname: Guo
authors.affiliation: Institute of Cardiovascular Disease, Key Laboratory for Arteriosclerology of Hunan, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Meng
authors.affiliation: Department of Basic Nursing, Yiyang Medical College, Yiyang, Hunan 413000, P.R. China.
authors.forename: Qingnan
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Cyrus Tang Hematology Center (Research Partnership), Jiangsu Institute of Hematology, The First Affiliated Hospital, Soochow University, Suzhou, Jiangsu 215400, P.R. China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Liu
authors.affiliation: Institute of Cardiovascular Disease, Key Laboratory for Arteriosclerology of Hunan, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Chaoke
authors.initials: C
authors.lastname: Tang
authors.affiliation: Institute of Cardiovascular Disease, Key Laboratory for Arteriosclerology of Hunan, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Guanghui
authors.initials: G
authors.lastname: Yi
authors.affiliation: Institute of Cardiovascular Disease, Key Laboratory for Arteriosclerology of Hunan, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Zuo
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Institute of Cardiovascular Disease, Key Laboratory for Arteriosclerology of Hunan, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Yin
authors.affiliation: Department of Cardiovascular Medicine, The Second Affiliated Hospital, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Guoping
authors.initials: G
authors.lastname: Tian
authors.affiliation: Institute of Cardiovascular Disease, Key Laboratory for Arteriosclerology of Hunan, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Yuan
mesh_terms: D000101:Acetyl-CoA C-Acetyltransferase / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002788:Cholesterol Esters / Q000378:metabolism*; D005786:Gene Expression Regulation; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D000070780:Perilipin-2; D051571:Protein Kinase C-alpha / Q000235:genetics / Q000378:metabolism*; D000067996:RAW 264.7 Cells; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D015398:Signal Transduction
chemical_list: D002788:Cholesterol Esters; D008077:Lipoproteins, LDL; D008565:Membrane Proteins; D000070780:Perilipin-2; C000602983:Plin2 protein, mouse; D034741:RNA, Small Interfering; C099252:oxidized low density lipoprotein; C506641:Acat1 protein, mouse; D000101:Acetyl-CoA C-Acetyltransferase; D051571:Protein Kinase C-alpha