title: Ethanol production from sugars obtained during enzymatic hydrolysis of elephant grass (Pennisetum purpureum, Schum.) pretreated by steam explosion.
abstract: In this work, steam explosion was used a pretreatment method to improve the conversion of elephant grass (Pennisetum purpureum) to cellulosic ethanol. This way, enzymatic hydrolysis of vaccum-drained and water-washed steam-treated substrates was carried out with Penicillium echinulatum enzymes while Saccharomyces cerevisiae CAT-1 was used for fermentation. After 48 h of hydrolysis, the highest yield of reducing sugars was obtained from vaccum-drained steam-treated substrates that were produced after 10 min at 200 °C (863.42 ± 62.52 mg/g). However, the highest glucose yield was derived from water-washed steam-treated substrates that were produced after 10 min at 190 °C (248.34 ± 6.27 mg/g) and 200 °C (246.00 ± 9.60 mg/g). Nevertheless, the highest ethanol production was obtained from water-washed steam-treated substrates that were produced after 6 min at 200 °C. These data revealed that water washing is a critical step for ethanol production from steam-treated elephant grass and that pretreatment generates a great deal of water soluble inhibitory compounds for hydrolysis and fermentation, which were partly characterized as part of this study.
authors.affiliation: University of Caxias do Sul, Enzyme and Biomass Laboratory, 1130 Francisco Vargas Street, Caxias do Sul, RS 95070-560, Brazil.
authors.forename: Angélica Luisi
authors.initials: AL
authors.lastname: Scholl
authors.affiliation: University of Caxias do Sul, Enzyme and Biomass Laboratory, 1130 Francisco Vargas Street, Caxias do Sul, RS 95070-560, Brazil.
authors.forename: Daiane
authors.initials: D
authors.lastname: Menegol
authors.affiliation: Federal University of Paraná, Department of Chemistry, Research Center in Applied Chemistry (CEPESQ), P.O. Box 19032, Curitiba, PR 81531-980, Brazil; Cane Technology Center (CTC), Fazenda Santo Antônio, Piracicaba, SP 13400-907, Brazil. Electronic address: ana.pitarelo@gmail.com.
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Pitarelo
authors.affiliation: University of Caxias do Sul, Enzyme and Biomass Laboratory, 1130 Francisco Vargas Street, Caxias do Sul, RS 95070-560, Brazil.
authors.forename: Roselei Claudete
authors.initials: RC
authors.lastname: Fontana
authors.affiliation: Federal University of Paraná, Department of Chemistry, Research Center in Applied Chemistry (CEPESQ), P.O. Box 19032, Curitiba, PR 81531-980, Brazil.
authors.forename: Arion
authors.initials: A
authors.lastname: Zandoná Filho
authors.affiliation: Federal University of Paraná, Department of Chemistry, Research Center in Applied Chemistry (CEPESQ), P.O. Box 19032, Curitiba, PR 81531-980, Brazil; INCT in Energy and Environment (INCT E&A), Federal University of Paraná, Department of Chemistry, Curitiba, PR 81531-980, Brazil. Electronic address: luiz.ramos@ufpr.br.
authors.forename: Luiz Pereira
authors.initials: LP
authors.lastname: Ramos
authors.affiliation: University of Caxias do Sul, Enzyme and Biomass Laboratory, 1130 Francisco Vargas Street, Caxias do Sul, RS 95070-560, Brazil.
authors.forename: Aldo José Pinheiro
authors.initials: AJ
authors.lastname: Dillon
authors.affiliation: University of Caxias do Sul, Enzyme and Biomass Laboratory, 1130 Francisco Vargas Street, Caxias do Sul, RS 95070-560, Brazil. Electronic address: mcamassola@gmail.com.
authors.forename: Marli
authors.initials: M
authors.lastname: Camassola
mesh_terms: D002241:Carbohydrates; D002480:Cellulase / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005107:Explosions; D005285:Fermentation / Q000502:physiology*; D006358:Hot Temperature; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism*; D010407:Penicillium / Q000378:metabolism; D031782:Pennisetum / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013227:Steam; D014867:Water / Q000737:chemistry
chemical_list: D002241:Carbohydrates; D013227:Steam; D014867:Water; C036909:lignocellulose; D000431:Ethanol; D008031:Lignin; D002480:Cellulase
keywords: Cellulosic ethanol; Elephant grass; Enzymatic hydrolysis; Fermentation; Steam explosion