title: Synergistic and Antagonistic Action of Phytochrome (Phy) A and PhyB during Seedling De-Etiolation in Arabidopsis thaliana.
abstract: It has been reported that Arabidopsis phytochrome (phy) A and phyB are crucial photoreceptors that display synergistic and antagonistic action during seedling de-etiolation in multiple light signaling pathways. However, the functional relationship between phyA and phyB is not fully understood under different kinds of light and in response to different intensities of such light. In this work, we compared hypocotyl elongation of the phyA-211 phyB-9 double mutant with the wild type, the phyA-211 and phyB-9 single mutants under different intensities of far-red (FR), red (R), blue (B) and white (W) light. We confirmed that phyA and phyB synergistically promote seedling de-etiolation in B-, B plus R-, W- and high R-light conditions. The correlation of endogenous ELONGATED HYPOCOTYL 5 (HY5) protein levels with the trend of hypocotyl elongation of all lines indicate that both phyA and phyB promote seedling photomorphogenesis in a synergistic manner in high-irradiance white light. Gene expression analyses of RBCS members and HY5 suggest that phyB and phyA act antagonistically on seedling development under FR light.
authors.affiliation: Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, China. suliang_mp5@163.com.|Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China. suliang_mp5@163.com.
authors.forename: Liang
authors.initials: L
authors.lastname: Su
authors.affiliation: Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China. biohoupei@gmail.com.
authors.forename: Pei
authors.initials: P
authors.lastname: Hou
authors.affiliation: Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China. szz628@126.com.|Beijing Radiation Center, Beijing 100875, China. szz628@126.com.
authors.forename: Meifang
authors.initials: M
authors.lastname: Song
authors.affiliation: Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China. hello_zx@hotmail.com.
authors.forename: Xu
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China. guolin@caas.cn.
authors.forename: Lin
authors.initials: L
authors.lastname: Guo
authors.affiliation: Graduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, China. xiaoyang@caas.cn.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China. yanlei2723@126.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Yan
authors.affiliation: Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, China. suliang_mp5@163.com.
authors.forename: Wanchen
authors.initials: W
authors.lastname: Li
authors.affiliation: Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China. yangjianping02@caas.cn.
authors.forename: Jianping
authors.initials: J
authors.lastname: Yang
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism; D003624:Darkness; D004307:Dose-Response Relationship, Radiation; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D018546:Hypocotyl / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects*; D008027:Light; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D051942:Phytochrome A / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D051939:Phytochrome B / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects
chemical_list: D029681:Arabidopsis Proteins; D050976:Basic-Leucine Zipper Transcription Factors; C109254:HY5 protein, Arabidopsis; D009687:Nuclear Proteins; C497973:PHYA protein, Arabidopsis; C497952:PHYB protein, Arabidopsis; D051942:Phytochrome A; D051939:Phytochrome B
keywords: Arabidopsis thaliana; antagonistic action; photomorphogenesis; phytochrome A; phytochrome B; synergistic action