title: The Origin, Development and Molecular Diversity of Rodent Olfactory Bulb Glutamatergic Neurons Distinguished by Expression of Transcription Factor NeuroD1.
abstract: Production of olfactory bulb neurons occurs continuously in the rodent brain. Little is known, however, about cellular diversity in the glutamatergic neuron subpopulation. In the central nervous system, the basic helix-loop-helix transcription factor NeuroD1 (ND1) is commonly associated with glutamatergic neuron development. In this study, we utilized ND1 to identify the different subpopulations of olfactory bulb glutamategic neurons and their progenitors, both in the embryo and postnatally. Using knock-in mice, transgenic mice and retroviral transgene delivery, we demonstrate the existence of several different populations of glutamatergic olfactory bulb neurons, the progenitors of which are ND1+ and ND1- lineage-restricted, and are temporally and regionally separated. We show that the first olfactory bulb glutamatergic neurons produced - the mitral cells - can be divided into molecularly diverse subpopulations. Our findings illustrate the complexity of neuronal diversity in the olfactory bulb and that seemingly homogenous neuronal populations can consist of multiple subpopulations with unique molecular signatures of transcription factors and expressing neuronal subtype-specific markers.
authors.affiliation: Stem Cell laboratory for CNS Disease Modeling, Wallenberg Neuroscience Center, Department of Experimental Medical Science, BMC A10, Lund University, 22184 Lund, Sweden; Strategic Research Area MultiPark, Lund University, 22184 Lund, Sweden; Lund Stem Cell Center, Lund University, 22184 Lund, Sweden.
authors.forename: Laurent
authors.initials: L
authors.lastname: Roybon
authors.affiliation: Department of Genetics and Development, Columbia University, 1150 St. Nicholas Ave., New York, New York, United States of America.
authors.forename: Teresa L
authors.initials: TL
authors.lastname: Mastracci
authors.affiliation: Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America.
authors.forename: Joyce
authors.initials: J
authors.lastname: Li
authors.affiliation: John van Geest Centre for Brain Repair, University of Cambridge, Forvie Site, Robinson Way, Cambridge CB2 0PY, United Kingdom.
authors.forename: Simon R W
authors.initials: SR
authors.lastname: Stott
authors.affiliation: Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America.
authors.forename: Andrew B
authors.initials: AB
authors.lastname: Leiter
authors.affiliation: Department of Genetics and Development, Columbia University, 1150 St. Nicholas Ave., New York, New York, United States of America.
authors.forename: Lori
authors.initials: L
authors.lastname: Sussel
authors.affiliation: Van Andel Research Institute, Center for Neurodegenerative Science, 333 Bostwick Avenue NE, Grand Rapids, Michigan 49503, United States of America.
authors.forename: Patrik
authors.initials: P
authors.lastname: Brundin
authors.affiliation: Neural Plasticity and Repair Unit, Wallenberg Neuroscience Center, Department of Experimental Medical Science, Lund University, BMC A10, 22184, Lund, Sweden.
authors.forename: Jia-Yi
authors.initials: JY
authors.lastname: Li
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D019070:Cell Lineage; D000066630:Ectopic Gene Expression; D018507:Gene Expression Regulation, Developmental; D018698:Glutamic Acid / Q000378:metabolism*; D020547:Lateral Ventricles / Q000166:cytology / Q000254:growth & development; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009830:Olfactory Bulb / Q000166:cytology* / Q000254:growth & development*; D020825:T-Box Domain Proteins / Q000378:metabolism
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D018698:Glutamic Acid; D009419:Nerve Tissue Proteins; D020825:T-Box Domain Proteins; C491198:Eomes protein, mouse; C093564:Neurogenic differentiation factor 1