title: Tanshinones suppress AURKA through up-regulation of miR-32 expression in non-small cell lung cancer.
abstract: Tanshinone is the liposoluble constituent of Salia miltiorrhiza, a root used in traditional herbal medicine which is known to possess certain health benefits. Although it is known that tanshinones, including tanshinone I (T1), tanshinone IIA (T2A), and cryptotanshinone (CT), can inhibit the growth of lung cancer cells in vitro, the mechanism under which they act is still unclear. AURKA, an oncogene, encodes a serine-threonine kinase which regulates mitotic processes in mammalian cells. Here, we reported that tanshinones mediate AURKA suppression partly through up-regulating the expression of miR-32. We found that tanshinones could inhibit cell proliferation, promote apoptosis, and impede cell-cycle progression, thus performing an antineoplastic function in non-small cell lung cancer (NSCLC). Additionally, we demonstrated that tanshinones attained these effects in part by down-regulating AURKA, corroborating previous reports. Our results showed that in NSCLC, similar effects were obtained with knock-down of the AURKA gene by siRNA. We also verified that AURKA was the direct target of miR-32. Collectively, our results demonstrated that tanshinones could inhibit NSCLC by suppressing AURKA via up-regulating the expressions of miR-32 and other related miRNAs.
authors.affiliation: School of Life Sciences, Shanghai University, Shanghai 200444, China.
authors.forename: Zhong-Liang
authors.initials: ZL
authors.lastname: Ma
authors.affiliation: School of Life Sciences, Shanghai University, Shanghai 200444, China.
authors.forename: Bing-Jie
authors.initials: BJ
authors.lastname: Zhang
authors.affiliation: School of Life Sciences, Shanghai University, Shanghai 200444, China.
authors.forename: D Tao
authors.initials: DT
authors.lastname: Wang
authors.affiliation: School of Life Sciences, Shanghai University, Shanghai 200444, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Life Sciences, Shanghai University, Shanghai 200444, China.
authors.forename: Jia-Li
authors.initials: JL
authors.lastname: Wei
authors.affiliation: School of Life Sciences, Shanghai University, Shanghai 200444, China.
authors.forename: Bo-Tao
authors.initials: BT
authors.lastname: Zhao
authors.affiliation: Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
authors.affiliation: School of Life Sciences, Shanghai University, Shanghai 200444, China.
authors.forename: Yan-Li
authors.initials: YL
authors.lastname: Li
authors.affiliation: School of Life Sciences, Shanghai University, Shanghai 200444, China.|State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
authors.forename: You-Xin
authors.initials: YX
authors.lastname: Jin
mesh_terms: D045784:Abietanes / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D064096:Aurora Kinase A / Q000037:antagonists & inhibitors*; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D057809:HEK293 Cells; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D045784:Abietanes; D000972:Antineoplastic Agents, Phytogenic; C552430:MIRN23a microRNA, human; D035683:MicroRNAs; C021751:tanshinone; C576917:AURKA protein, human; D064096:Aurora Kinase A
keywords: AURKA; miRNA; non-small cell lung cancer; tanshinone