title: Mechanical properties and in vivo healing evaluation of a novel Centella asiatica-loaded hydrocolloid wound dressing.
abstract: To develop a novel sodium alginate based Centella asiatica (CA)-loaded hydrocolloid wound dressing (HCD) providing excellent mechanical properties and improved wound healing, numerous CA-loaded HCDs were prepared with various ingredients using the hot melting method. The effect of sodium alginate, styrene-isoprene-styrene copolymer (SIS) and petroleum hydrocarbon resin (PHR) on the mechanical properties of CA-loaded HCDs was investigated. The effect of disintegrants on swelling and drug release was assessed. Moreover, the in vivo wound healing potentials of the selected CA-loaded HCD in various wound models such as abrasion, excision and infection were evaluated in comparison with the commercial product. Polyisobutylene and SIS hardly affected the mechanical properties, but PHR improved the tensile strength and elongation at break. Disintegrants such as croscarmellose sodium, sodium starch glycolate and crospovidone improved the swelling ratio of the CA-loaded HCD. Furthermore, the CA-loaded HCD without croscarmellose sodium poorly released the drug, but that with 2% croscarmellose sodium showed about 27% drug release in 24h. In particular, the CA-loaded HCD composed of CA/polyisobutylene/SIS/PHR/liquid paraffin/sodium alginate/croscarmellose sodium at the weight ratio of 1/8/25/25/12/27/2 furnished excellent mechanical properties and drug release. As compared with the commercial product, it offered improved healing effects in excision, infection and abrasion type wounds in rats. Thus, this novel CA-loaded HCD could be a potential candidate for the treatment of various wounds.
authors.affiliation: College of Pharmacy & Institute of Pharmaceutical Science and Technology, Hanyang University, 55Hanyangdaehak-ro, Sangnok-gu, Ansan 426-791, South Korea.
authors.forename: Sung Giu
authors.initials: SG
authors.lastname: Jin
authors.affiliation: College of Pharmacy & Institute of Pharmaceutical Science and Technology, Hanyang University, 55Hanyangdaehak-ro, Sangnok-gu, Ansan 426-791, South Korea.
authors.forename: Kyung Soo
authors.initials: KS
authors.lastname: Kim
authors.affiliation: College of Pharmacy & Institute of Pharmaceutical Science and Technology, Hanyang University, 55Hanyangdaehak-ro, Sangnok-gu, Ansan 426-791, South Korea.
authors.forename: Abid Mehmood
authors.initials: AM
authors.lastname: Yousaf
authors.affiliation: College of Pharmacy & Institute of Pharmaceutical Science and Technology, Hanyang University, 55Hanyangdaehak-ro, Sangnok-gu, Ansan 426-791, South Korea.
authors.forename: Dong Wuk
authors.initials: DW
authors.lastname: Kim
authors.affiliation: Pharmaceutical Product Research Laboratories, Dong-A Pharm. Co., Ltd., Yongin-Si, Kyunggi-Do 449-905, South Korea.
authors.forename: Sun Woo
authors.initials: SW
authors.lastname: Jang
authors.affiliation: Pharmaceutical Product Research Laboratories, Dong-A Pharm. Co., Ltd., Yongin-Si, Kyunggi-Do 449-905, South Korea.
authors.forename: Mi-Won
authors.initials: MW
authors.lastname: Son
authors.affiliation: College of Pharmacy & Institute of Pharmaceutical Science and Technology, Hanyang University, 55Hanyangdaehak-ro, Sangnok-gu, Ansan 426-791, South Korea.
authors.forename: Young Hun
authors.initials: YH
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Yeungnam University, 214-1, Dae-Dong, Gyongsan 712-749, South Korea.
authors.forename: Chul Soon
authors.initials: CS
authors.lastname: Yong
authors.affiliation: College of Pharmacy, Yeungnam University, 214-1, Dae-Dong, Gyongsan 712-749, South Korea. Electronic address: jongohkim@yu.ac.kr.
authors.forename: Jong Oh
authors.initials: JO
authors.lastname: Kim
authors.affiliation: College of Pharmacy & Institute of Pharmaceutical Science and Technology, Hanyang University, 55Hanyangdaehak-ro, Sangnok-gu, Ansan 426-791, South Korea. Electronic address: hangon@hanyang.ac.kr.
authors.forename: Han-Gon
authors.initials: HG
authors.lastname: Choi
mesh_terms: D000464:Alginates / Q000737:chemistry; D000818:Animals; D001458:Bandages; D002266:Carboxymethylcellulose Sodium / Q000737:chemistry; D028041:Centella / Q000737:chemistry*; D003102:Colloids / Q000737:chemistry* / Q000494:pharmacology*; D003432:Cross-Linking Reagents / Q000737:chemistry; D005079:Excipients / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006838:Hydrocarbons / Q000737:chemistry; D008297:Male; D010578:Petroleum; D010936:Plant Extracts; D011090:Polyenes / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D011205:Povidone / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D013718:Tensile Strength; D013729:Terpenes / Q000737:chemistry; D014315:Triterpenes / Q000737:chemistry* / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000464:Alginates; C526146:Centella asiatica extract; D003102:Colloids; D003432:Cross-Linking Reagents; D005079:Excipients; D006603:Hexuronic Acids; D006838:Hydrocarbons; D010578:Petroleum; D010936:Plant Extracts; D011090:Polyenes; D011108:Polymers; D011137:Polystyrenes; D013729:Terpenes; D014315:Triterpenes; C575353:styrene-isoprene-styrene block copolymer; D020723:Glucuronic Acid; C008967:polyisobutylene; D011205:Povidone; D002266:Carboxymethylcellulose Sodium
keywords: Centella asiatica-loaded wound dressing; Croscarmellose sodium; Elongation at break; Sodium alginate; Swelling ratio; Tensile strength; Wound healing