title: No Association between the CCR5Δ32 Polymorphism and Sporadic Esophageal Cancer in Punjab, North-West India.
abstract: BACKGROUND
  Chemokines and their receptors influence carcinogenesis and cysteine-cysteine chemokine receptor 5 (CCR5) directs spread of cancer to other tissues. A 32 base pair deletion in the coding region of CCR5 that might alter the expression or function of the protein has been implicated in a variety of immune-mediated diseases. The action of antiviral drugs being proposed as adjuvant therapy in cancer is dependent on CCR5 wild type status. In the present study, distribution of CCR5Δ32 polymorphism was assessed in North Indian esophageal cancer patients to explore the potential of using chemokine receptors antagonists as adjuvant therapy.
  
  
  METHODS
  DNA samples of 175 sporadic esophageal cancer patients (69 males and 106 females) and 175 unrelated healthy control individuals (69 males and 106 females) were screened for the CCR5Δ32 polymorphism by direct polymerase chain reaction (PCR).
  
  
  RESULTS
  The frequencies of wild type homozygous (CCR5/CCR5), heterozygous (CCR5/Δ32) and homozygous mutant (Δ32/Δ32) genotypes were 96.0 vs 97.72%, 4.0 vs 1.71% and 0 vs 0.57% in patients and controls respectively. There was no difference in the genotype and allele frequencies of CCR5Δ32 polymorphism in esophageal cancer patients and control group.
  
  
  CONCLUSIONS
  The CCR5Δ32 polymorphism is not associated with esophageal cancer in North Indians. As the majority of patients express the wild type allele, there is potential of using antiviral drug therapy as adjuvant therapy.
authors.affiliation: Department of Human Genetics, Human Cytogenetics Laboratory, Guru Nanak Dev University, Punjab, India E-mail : guleria_k@yahoo.com.
authors.forename: Vasudha
authors.initials: V
authors.lastname: Sambyal
authors.forename: Mridu
authors.initials: M
authors.lastname: Manjari
authors.forename: Meena
authors.initials: M
authors.lastname: Sudan
authors.forename: Manjit Singh
authors.initials: MS
authors.lastname: Uppal
authors.forename: Neeti Rajan
authors.initials: NR
authors.lastname: Singh
authors.forename: Harpreet
authors.initials: H
authors.lastname: Singh
authors.forename: Kamlesh
authors.initials: K
authors.lastname: Guleria
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D016022:Case-Control Studies; D004938:Esophageal Neoplasms / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D007194:India; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D019713:Receptors, CCR5 / Q000235:genetics*; D017384:Sequence Deletion; D055815:Young Adult
chemical_list: C584933:CCR5 protein, human; D019713:Receptors, CCR5