title: Effect of Monocyte-Like THP-1 Cells on the Formation of Vascular Tubes by EA.Hy926s Endothelial Cells in the Presence of Cytokines.
abstract: The interaction of endothelial cells with cells of the microenvironment, including monocytes/ macrophages, and extracellular matrix during angiogenesis is controlled by cytokines. The stimulating effect bFGF, IL-8, and VEGF on the formation of capillary-like structures by endothelial cells was demonstrated in both monoculture and in co-culture with THP-1 cells; in the latter case, the effects of bFGF and VEGF were more pronounced. IL-8 reduced branching of vascular tubes in co-culture in comparison with monoculture of endothelial cells. Placental growth factor PlGF had no effect of tube formation by endothelial cells in monoculture, but in co-culture with THP-1 cells this cytokine in high concentrations exhibited proangiogenic activity. TGFb inhibited the formation of vascular tubes by endothelial cells and its antiangiogenic potential was more pronounced in co-culture with THP-1 cells.
authors.affiliation: D. O. Ott Research Institute of Obstetrics and Gynecology, North-Western Division of the Russian Academy of Medical Sciences, St. Petersburg, Russia.
authors.forename: T Yu
authors.initials: TY
authors.lastname: L'vova
authors.forename: O I
authors.initials: OI
authors.lastname: Stepanova
authors.forename: L S
authors.initials: LS
authors.lastname: Okorokova
authors.forename: V A
authors.initials: VA
authors.lastname: Semenov
authors.forename: E A
authors.initials: EA
authors.lastname: Rebezova
authors.forename: S A
authors.initials: SA
authors.lastname: Sel'kov
authors.forename: D I
authors.initials: DI
authors.lastname: Sokolov
mesh_terms: D002196:Capillaries / Q000254:growth & development*; D002454:Cell Differentiation; D002460:Cell Line; D018920:Coculture Techniques; D042783:Endothelial Cells / Q000166:cytology*; D004730:Endothelium, Vascular / Q000254:growth & development*; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D006801:Humans; D016209:Interleukin-8 / Q000494:pharmacology; D009000:Monocytes / Q000166:cytology*; D018919:Neovascularization, Physiologic / Q000502:physiology*; D000072483:Placenta Growth Factor; D011257:Pregnancy Proteins / Q000494:pharmacology; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology
chemical_list: C508606:CXCL8 protein, human; D016209:Interleukin-8; C000608036:PGF protein, human; D011257:Pregnancy Proteins; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D000072483:Placenta Growth Factor; C108111:fibroblast growth factor 13; D005346:Fibroblast Growth Factors