title: Effects of maternal subtotal nephrectomy on the development of the fetal kidney: A morphometric study.
abstract: The present study was designed to explore if maternal subtotal (5/6) nephrectomy affects the development of fetal rat kidneys using morphometric methods and examining whether there are any apoptotic changes in the fetal kidney. To generate 5/6 nephrectomized model rats, animals underwent 2/3 left nephrectomy on gestation day (GD) 5 and total right nephrectomy on GD 12. The fetal kidneys were examined on GDs 16 and 22. A significant decrease in fetal body weight resulting from maternal 5/6 nephrectomy was observed on GD 16, and a significant decrease in fetal renal weight and fetal body weight caused by maternal nephrectomy was observed on GD 22. Maternal 5/6 nephrectomy induced a significant increase in glomerular number, proximal tubular length, and total proximal tubular volume of fetuses on GD 22. Maternal 5/6 nephrectomy resulted in an increase in the number of apoptotic cells in the metanephric mesenchyme of the kidney on GD 16, and in the collecting tubules on GD 22. These findings suggest that maternal 5/6 nephrectomy stimulates the development of the fetal kidney while suppressing fetal growth.
authors.affiliation: Department of Integrated Structural Biosciences, Division of Veterinary Science, Graduate School of Life and Environmental Biosciences, Osaka Prefecture University, Osaka, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Kondo
authors.affiliation: Department of Integrated Structural Biosciences, Division of Veterinary Science, Graduate School of Life and Environmental Biosciences, Osaka Prefecture University, Osaka, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Kitano-Amahori
authors.affiliation: Department of Integrated Structural Biosciences, Division of Veterinary Science, Graduate School of Life and Environmental Biosciences, Osaka Prefecture University, Osaka, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Nagai
authors.affiliation: Department of Integrated Structural Biosciences, Division of Veterinary Science, Graduate School of Life and Environmental Biosciences, Osaka Prefecture University, Osaka, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Mino
authors.affiliation: Department of Integrated Structural Biosciences, Division of Veterinary Science, Graduate School of Life and Environmental Biosciences, Osaka Prefecture University, Osaka, Japan.
authors.forename: Ai
authors.initials: A
authors.lastname: Takeshita
authors.affiliation: Department of Veterinary Anatomy, Faculty of Common Veterinary Medicine, University of Yamaguchi, Yoshida, Japan.
authors.forename: Ken Takeshi
authors.initials: KT
authors.lastname: Kusakabe
authors.affiliation: Department of Integrated Structural Biosciences, Division of Veterinary Science, Graduate School of Life and Environmental Biosciences, Osaka Prefecture University, Osaka, Japan.
authors.forename: Toshiya
authors.initials: T
authors.lastname: Okada
mesh_terms: D000818:Animals; D017209:Apoptosis; D015415:Biomarkers; D001835:Body Weight; D005260:Female; D047109:Fetal Development; D007150:Immunohistochemistry; D007668:Kidney / Q000033:anatomy & histology* / Q000254:growth & development* / Q000473:pathology; D007678:Kidney Glomerulus / Q000166:cytology / Q000378:metabolism; D018811:Maternal Exposure; D023421:Models, Animal; D009392:Nephrectomy; D009929:Organ Size; D011247:Pregnancy; D051381:Rats
chemical_list: D015415:Biomarkers
keywords: fetal rat kidney; glomerulus; maternal subtotal nephrectomy; morphometry; proximal tubules