title: Successful production of piglets derived from mature oocytes vitrified using OPS method.
abstract: OBJECTIVE
  Examination of effect of vitrification solution with or without foetal calf serum (FCS) on the in vitro and in vivo survival of matured pig oocytes.
  
  
  METHODS
  Exp. 1: oocytes were exposed to vitrification solutions: VSa (15% DMSO, 15% EG, 0.5 M sucrose dissolved in TCM-199 with FCS) or VSb (VSa without FCS). Exp. 2: oocytes were vitrified in VSa or VSb using OPS. A fraction of vitrified oocytes were transferred to 6 synchronised and inseminated recipients..
  
  
  RESULTS
  Survival rate after exposure and vitrification was the same for VSa and VSb. Transfer of 48 oocytes vitrified in VSb resulted with two pregnancies and 12 live piglets. Molecular analysis results: eight piglets originated from the surrogate mother's oocytes, four piglets from vitrified oocytes..
  
  
  CONCLUSIONS
  The use of DMSO and EG as cryoprotectants without serum supplementation was advantageous for the in vivo development of vitrified mature porcine oocytes.
authors.forename: Barbara
authors.initials: B
authors.lastname: Gajda
authors.forename: Marzena
authors.initials: M
authors.lastname: Skrzypczak-Zielińska
authors.forename: Barbara
authors.initials: B
authors.lastname: Gawrońska
authors.forename: Ryszard
authors.initials: R
authors.lastname: Słomski
authors.forename: Zdzisław
authors.initials: Z
authors.lastname: Smorąg
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002470:Cell Survival / Q000187:drug effects; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000378:metabolism; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods / Q000662:veterinary*; D009865:Oocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D013552:Swine / Q000502:physiology*; D058989:Vitrification
chemical_list: D003451:Cryoprotective Agents