title: A polysaccharide fromDendrobium huoshanenseprevents hepatic inflammatory response caused by carbon tetrachloride.
abstract: Dendrobium huoshanenseis a precious herbal medicine in China, which exhibits a variety of restorative and therapeutic effects. This study aimed at investigating the hepatoprotective effects of a polysaccharide (DHP1A) isolated fromD. huoshanensevia water extraction, diethylaminoethyl (DEAE) cellulose anion exchange and size exclusion chromatography. The animal experiment indicated that the oral administration of DHP1A obviously reduced the levels of alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase and 8-hydroxy-2'-deoxyguanosine in the serum of mice treated with carbon tetrachloride (CCl4), suggesting the hepatoprotective potential of this polysaccharide. Moreover, DHP1A decreased the expressions of tumor necrosis factor-α, interleukin-1β, monocyte chemoattractant protein-1, macrophage inflammatory protein-2, CD68 and phosphorylated IκBα (p-IκBα) in the CCl4-treated mice. These results revealed that the hepatoprotective effect of DHP1A was partly attributed to its anti-inflammatory action.
authors.affiliation: Hefei University of Technology, School of Biotechnology and Food Engineering , Hefei , China ; Bengbu College, Department of Biotechnology and Food Engineering , Bengbu , China.
authors.forename: Chang-Cheng
authors.initials: CC
authors.lastname: Tian
authors.affiliation: Hefei University of Technology, School of Biotechnology and Food Engineering , Hefei , China.
authors.forename: Xue-Qiang
authors.initials: XQ
authors.lastname: Zha
authors.affiliation: Hefei University of Technology, School of Biotechnology and Food Engineering , Hefei , China.
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Luo
keywords: Dendrobium huoshanense; anti-inflammation; hepatoprotection; polysaccharide