title: A three-dimensional model of primary bovine endometrium using an electrospun scaffold.
abstract: Endometrial stromal and epithelial cell function is typically studied in vitro using standard two-dimensional monocultures, but these cultures fail to reflect the complex three-dimensional (3D) architecture of tissue. A 3D model of bovine endometrium that reflects the architectural arrangement of in vivo tissue would beneficially assist the study of tissue function. An electrospun polyglycolide (PGA) scaffold was selected to grow a 3D model of primary bovine endometrial epithelial and stromal cells, that reflects the architecture of the endometrium for the study of pathophysiology. Electrospun scaffolds were seeded with stromal and epithelial cells, and growth was assessed using histological techniques. Prostaglandin E2 and prostaglandin F2α responsiveness of endometrial scaffold constructs was tested using oxytocin plus arachidonic acid (OT + AA) or lipopolysaccharide (LPS). Stromal and epithelial cells growing on the electrospun scaffold had an architectural arrangement that mimicked whole tissue, deposited fibronectin, had appropriate expression of vimentin and cytokeratin and were responsive to OT + AA and LPS, as measured by prostaglandin accumulation. In conclusion, a functional 3D model of stromal and epithelial cells was developed using a PGA electrospun scaffold which may be used to study endometrial pathophysiology.
authors.affiliation: Institute of Life Science, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, UK. Department of Veterinary Clinical Sciences, Royal Veterinary College, Royal College Street, London NW1 0TU, UK.
authors.forename: S B
authors.initials: SB
authors.lastname: MacKintosh
authors.forename: L P
authors.initials: LP
authors.lastname: Serino
authors.forename: P D
authors.initials: PD
authors.lastname: Iddon
authors.forename: R
authors.initials: R
authors.lastname: Brown
authors.forename: R S
authors.initials: RS
authors.lastname: Conlan
authors.forename: C J
authors.initials: CJ
authors.lastname: Wright
authors.forename: T G G
authors.initials: TG
authors.lastname: Maffeis
authors.forename: M J
authors.initials: MJ
authors.lastname: Raxworthy
authors.forename: I M
authors.initials: IM
authors.lastname: Sheldon
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000633:toxicity; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D018920:Coculture Techniques; D015237:Dinoprost / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D004717:Endometrium / Q000166:cytology* / Q000503:physiopathology; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005260:Female; D007150:Immunohistochemistry; D007633:Keratins / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity; D010121:Oxytocin / Q000494:pharmacology; D011100:Polyglycolic Acid / Q000737:chemistry* / Q000633:toxicity; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D054457:Tissue Scaffolds; D014746:Vimentin / Q000378:metabolism
chemical_list: D008070:Lipopolysaccharides; D014746:Vimentin; D011100:Polyglycolic Acid; D016718:Arachidonic Acid; D010121:Oxytocin; D007633:Keratins; D015237:Dinoprost; D015232:Dinoprostone