title: Carboxymethylated hyperbranched polysaccharide: Synthesis, solution properties, and fabrication of hydrogel.
abstract: The periphery of hyperbranched polysaccharides has many end groups that can be functioned and used as sites to interact with their surroundings. A water-insoluble hyperbranched β-d-glucan, coded as TM3a, extracted from sclerotia of an edible fungus (Pleurotus tuber-regium), was fractionated and modified chemically to obtain carboxymethylated derivatives (CTM3a). The solution properties of the carboxymethylated polysaccharides were studied systematically in phosphate buffer saline at 37 °C. The results indicated that the carboxymethylated glucans still kept hyperbranched structure after carboxymethylation, and existed as a swollen sphere-like chain conformation. The introduction of carboxymethylated groups permitted the formation of hydrogels through crosslinking CTM3a and silk fibroin with carbodiimide chemistry. The resultant hydrogels with porous and interconnected structure displayed good mechanical and swelling properties. This work provides some valuable and fundamental information of the natural hyperbranched polysaccharide from mushroom for further application in biomedical devices and tissue engineering.
authors.affiliation: Key Laboratory of Green Processing and Functional Textiles of New Textile Materials, Ministry of Education, Wuhan Textile University, Wuhan 430073, China. Electronic address: yongzhentao@126.com.
authors.forename: Yongzhen
authors.initials: Y
authors.lastname: Tao
authors.affiliation: College of Mathematics and Computer Science, Wuhan Textile University, Wuhan 430073, China.
authors.forename: Ruquan
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Green Processing and Functional Textiles of New Textile Materials, Ministry of Education, Wuhan Textile University, Wuhan 430073, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yang
authors.affiliation: Key Laboratory of Green Processing and Functional Textiles of New Textile Materials, Ministry of Education, Wuhan Textile University, Wuhan 430073, China.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Liu
authors.affiliation: Key Laboratory of Green Processing and Functional Textiles of New Textile Materials, Ministry of Education, Wuhan Textile University, Wuhan 430073, China.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Yang
authors.affiliation: Key Laboratory of Green Processing and Functional Textiles of New Textile Materials, Ministry of Education, Wuhan Textile University, Wuhan 430073, China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Zhao
mesh_terms: D005349:Fibroins / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D020076:Pleurotus; D016062:Porosity; D012995:Solubility; D047071:beta-Glucans / Q000737:chemistry*
chemical_list: D020100:Hydrogels; D047071:beta-Glucans; D005349:Fibroins
keywords: Carboxymethylated hyperbranched polysaccharide; Chain conformation; Hydrogel; Silk fibroin