title: High-throughput screening for a moderately halophilic phenol-degrading strain and its salt tolerance response.
abstract: A high-throughput screening system for moderately halophilic phenol-degrading bacteria from various habitats was developed to replace the conventional strain screening owing to its high efficiency. Bacterial enrichments were cultivated in 48 deep well microplates instead of shake flasks or tubes. Measurement of phenol concentrations was performed in 96-well microplates instead of using the conventional spectrophotometric method or high-performance liquid chromatography (HPLC). The high-throughput screening system was used to cultivate forty-three bacterial enrichments and gained a halophilic bacterial community E3 with the best phenol-degrading capability. Halomonas sp. strain 4-5 was isolated from the E3 community. Strain 4-5 was able to degrade more than 94% of the phenol (500 mg · L(-1) starting concentration) over a range of 3%-10% NaCl. Additionally, the strain accumulated the compatible solute, ectoine, with increasing salt concentrations. PCR detection of the functional genes suggested that the largest subunit of multicomponent phenol hydroxylase (LmPH) and catechol 1,2-dioxygenase (C12O) were active in the phenol degradation process.
authors.affiliation: State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China. lzy1009a@163.com.
authors.forename: Zhi-Yan
authors.initials: ZY
authors.lastname: Lu
authors.affiliation: State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China. foreverkimi1017@126.com.
authors.forename: Xiao-Jue
authors.initials: XJ
authors.lastname: Guo
authors.affiliation: State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China. huili@ecust.edu.cn.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China. z2cyj@163.com.|School of Chemical Engineering, Shanghai University of Engineering Science, Shanghai 201620, China. z2cyj@163.com.
authors.forename: Zhong-Zi
authors.initials: ZZ
authors.lastname: Huang
authors.affiliation: State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China. kflin@ecust.edu.cn.
authors.forename: Kuang-Fei
authors.initials: KF
authors.lastname: Lin
authors.affiliation: State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China. ydliu@ecust.edu.cn.
authors.forename: Yong-Di
authors.initials: YD
authors.lastname: Liu
mesh_terms: D000599:Amino Acids, Diamino / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics; D001673:Biodegradation, Environmental; D050548:Catechol 1,2-Dioxygenase / Q000235:genetics; D044129:Halomonadaceae / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology*; D057166:High-Throughput Screening Assays / Q000379:methods*; D006899:Mixed Function Oxygenases / Q000235:genetics; D010636:Phenols / Q000378:metabolism*; D055049:Salt Tolerance
chemical_list: D000599:Amino Acids, Diamino; D001426:Bacterial Proteins; D010636:Phenols; C045628:ectoine; D006899:Mixed Function Oxygenases; D050548:Catechol 1,2-Dioxygenase; C022336:phenol 2-monooxygenase
keywords: high-throughput screening; moderately halophilic bacteria; phenol-degrading; salt tolerance