title: Expression of a manganese peroxidase isozyme 2 transgene in the ethanologenic white rot fungus Phlebia sp. strain MG-60.
abstract: BACKGROUND
  The white-rot fungus Phlebia sp. strain MG-60 was proposed as a candidate for integrated fungal fermentation process (IFFP), which unifies aerobic delignification and semi-aerobic consolidated biological processing by a single microorganism based on its ability to efficiently degrade lignin and ferment the sugars from cellulose. To improve IFFP, the development of a molecular breeding method for strain MG-60 is necessary. The purpose of this study is to establish the transformation method for the strain MG-60 and to obtain the over-expressing transformants of lignin-degrading enzyme, manganese peroxidase.
  
  
  RESULTS
  In the present study, the expression vector regulated by Phlebia brevispora glyceraldehyde-3-phosphate dehydrogenase promoter and terminator was constructed. A polyethylene glycol transformation method for the ethanol-fermenting white-rot fungus Phlebia sp. MG-60 was established with high transformation efficiency, and the manganese peroxidase isozyme 2 gene (MGmnp2) transformants were obtained, showing higher MnP activity than control transformants. MGmnp2 transformants showed higher selective lignin degradation on Quercus wood powder.
  
  
  CONCLUSIONS
  This first report of MG-60 transformation provides a useful methodology for widely accessible to interested researches. These results indicate the possibility of metabolic engineering of strain MG-60 for improving IFFP.
authors.affiliation: Center of Community Organization, University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki, 889-2192 Japan ; Department of Forest and Environmental Sciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki, 889-2192 Japan.
authors.forename: Yumi
authors.initials: Y
authors.lastname: Yamasaki
authors.affiliation: Department of Forest and Environmental Sciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki, 889-2192 Japan.
authors.forename: Megumi
authors.initials: M
authors.lastname: Yamaguchi
authors.affiliation: Planning and Promotion Section, NARO Tohoku Agricultural Research Center for Tohoku Region, Morioka, 020-0198 Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Yamagishi
authors.affiliation: Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Shizuoka, 422-8529 Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Hirai
authors.affiliation: Department of Agro-Environmental Sciences, Faculty of Agriculture, Kyushu University, Fukuoka, 812-8581 Japan.
authors.forename: Ryuichiro
authors.initials: R
authors.lastname: Kondo
authors.affiliation: Department of Forest and Environmental Sciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki, 889-2192 Japan.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Kamei
authors.affiliation: Department of Forest and Environmental Sciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki, 889-2192 Japan.
authors.forename: Sadatoshi
authors.initials: S
authors.lastname: Meguro
keywords: Lignin; Manganese peroxidase; Phlebia sp. MG-60; White rot fungus