title: Characterization of the antibody response against EV71 capsid proteins in Chinese individuals by NEIBM-ELISA.
abstract: Human enterovirus 71 (EV71) has become the major pathogen of hand, foot, and mouth disease (HFMD) worldwide, while the anti-EV71 antibody responses other than neutralizing epitopes have not been characterized. In this study, EV71 capsid proteins VP1, VP3, VP0 and various VP1 antigens were constructed to analyze anti-EV71 response in severe HFMD cases, non-HFMD outpatient children and normal adults using a novel evolved immunoglobulin-binding molecule (NEIBM)-based ELISA. The high prevalence of antibody responses against all three capsid proteins was demonstrated, and anti-EV71 VP1 showed the main antibody response. Anti-EV71 VP1 antibody response was found to predominantly target to epitopes based on the common enterovirus cross-reactive sequence. Moreover, inhibition pattern against anti-EV71 VP1 reactions in three groups was obviously different. Taken together, these results firstly characterized the anti-EV71 antibody responses which are predominantly against VP1 epitopes based on common enterovirus cross-reactive sequence. This finding could be helpful for the better understanding of anti-EV71 humoral immunity and useful for seroepidemiological surveillance.
authors.affiliation: Department of Medical Microbiology and Parasitology, School of Basic Medicine, Second Military Medical University.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Department of pediatrics, Wuxi people's hospital, Jiangsu, China.
authors.forename: Xuguang
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Blood Transfusion, Changhai Hospital, Second Military Medical University, Shanghai, China.
authors.forename: Baohua
authors.initials: B
authors.lastname: Qian
authors.affiliation: Department of Clinical Laboratory, Wuxi people's hospital, Jiangsu, China.
authors.forename: Guorong
authors.initials: G
authors.lastname: Wu
authors.affiliation: Department of Medical Microbiology and Parasitology, School of Basic Medicine, Second Military Medical University.
authors.forename: Ting
authors.initials: T
authors.lastname: He
authors.affiliation: Department of Medical Microbiology and Parasitology, School of Basic Medicine, Second Military Medical University.
authors.forename: Jiaojiao
authors.initials: J
authors.lastname: Feng
authors.affiliation: Department of Medical Microbiology and Parasitology, School of Basic Medicine, Second Military Medical University.
authors.forename: Caixia
authors.initials: C
authors.lastname: Gao
authors.affiliation: Department of Medical Microbiology and Parasitology, School of Basic Medicine, Second Military Medical University.
authors.forename: Lili
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Medical Microbiology and Parasitology, School of Basic Medicine, Second Military Medical University.
authors.forename: Jinhong
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Medical Microbiology and Parasitology, School of Basic Medicine, Second Military Medical University.
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Medical Microbiology and Parasitology, School of Basic Medicine, Second Military Medical University.
authors.forename: Mingmei
authors.initials: M
authors.lastname: Cao
authors.affiliation: Department of Medical Microbiology and Parasitology, School of Basic Medicine, Second Military Medical University.
authors.forename: Heng
authors.initials: H
authors.lastname: Peng
authors.affiliation: Department of Medical Microbiology and Parasitology, School of Basic Medicine, Second Military Medical University.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Department of Medical Microbiology and Parasitology, School of Basic Medicine, Second Military Medical University.
authors.forename: Wei
authors.initials: W
authors.lastname: Pan
mesh_terms: D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology; D036022:Capsid Proteins / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D003429:Cross Reactions / Q000276:immunology; D029821:Enterovirus A, Human / Q000276:immunology*; D004769:Enterovirus Infections / Q000453:epidemiology* / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D000939:Epitopes / Q000276:immunology; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D011994:Recombinant Proteins / Q000276:immunology; D016036:Seroepidemiologic Studies; D055815:Young Adult
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D000939:Epitopes; D011994:Recombinant Proteins