title: Magnetic solid-phase extraction for determination of sulpiride in human urine and blood using high-performance liquid chromatography.
abstract: A novel and efficient sample preconcentration technique based on the Fe3O4 magnetic nanoparticles (Fe3O4 MNPs) coated with silica (SiO2) has been developed for extraction and determination of sulpiride. The functionalized MNPs showed excellent dispersibility in aqueous solution and were applied to magnetic solid-phase extraction of sulpiride from human urine and blood prior to high-performance liquid chromatography analysis. The separation, preconcentration and desorption procedure was completed in 10 min. Optimal experimental conditions, including sample pH, the amount of the MNPs, eluent type and volume, and the ultrasonication time were studied and established. The method showed good linearity for the determination of sulpiride in the concentration range of 10-1000 ng/mL in urine and blood. The recovery of the method was in the range between 91.2 and 97.5%, and the limit of detection was 2 ng/mL for sulpiride in human blood and urine. The results indicated that the present procedure is a suitable pretreatment method for biological samples.
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China.|Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming, 650500, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China.
authors.forename: Wenlong
authors.initials: W
authors.lastname: Liao
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China.
authors.forename: Yaling
authors.initials: Y
authors.lastname: Yang
mesh_terms: D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*; D013469:Sulpiride / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification / Q000652:urine*
chemical_list: D058185:Magnetite Nanoparticles; D013469:Sulpiride
keywords: Fe3O4 magnetic nanoparticles; blood; high performance liquid chromatography; sulpiride; urine