title: Effect of Vitamin D on basal and Luteinizing Hormone (LH) induced testosterone production and mitochondrial dehydrogenase activity in cultured Leydig cells from immature and mature rams.
abstract: The objectives of this study were to investigate the potential effects of 1α,25-(OH)2VD3 (biologically active form of Vitamin D) on basal and LH-induced testosterone production and mitochondrial dehydrogenase activity in Leydig cells from immature and mature rams cultured in vitro. Leydig cells were isolated from testes of immature and mature rams, treated without (control) or with increasing concentrations of LH (1, 10, 100ng/ml) and/or 1α,25-(OH)2VD3 (1, 10, 100nM). After 24h, concentrations of testosterone in culture media were measured. After 96h, mitochondrial dehydrogenase activity in Leydig cells were measured. In immature and mature ram Leydig cells, treatment with 10 and 100ng/ml LH increased testosterone production and mitochondrial dehydrogenase activity. Treatment with 1α,25-(OH)2VD3 in the absence of LH did not increase testosterone production, but 10 and 100nM 1α,25-(OH)2VD3 increased LH induced testosterone production for both immature and mature ram Leydig cells. Treatment with all doses of 1α,25-(OH)2VD3 in the absence of LH and 10 and 100ng/ml LH in the absence of 1α,25-(OH)2VD3 increased mitochondrial dehydrogenase activity for cultured Leydig cells from immature and mature rams and 1 and 10nM 1α,25-(OH)2VD3 treatment enhanced the LH induced increase in mitochondrial dehydrogenase activity. Result demonstrate Vitamin D3 induced regulation of function of Leydig cells from immature and mature rams cultured in the presence or absence of LH and support a potential role for Vitamin D3 in regulation of gonadal function in rams.
authors.affiliation: College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Huang
authors.affiliation: College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Jin
authors.affiliation: College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Chen
authors.affiliation: College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Jiang
authors.affiliation: College of Life Science, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
authors.forename: Youshe
authors.initials: Y
authors.lastname: Ren
authors.affiliation: College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
authors.forename: Wenzhong
authors.initials: W
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi 030801, China; Division of Animal and Nutritional Sciences, West Virginia University, Morgantown, WV 26506, United States.
authors.forename: Jianbo
authors.initials: J
authors.lastname: Yao
authors.affiliation: Laboratory of Mammalian Reproductive Biology and Genomics, Michigan State University, East Lansing, MI 48824, United States; Department of Animal Science, Michigan State University, East Lansing, MI 48824, United States.
authors.forename: Joseph K
authors.initials: JK
authors.lastname: Folger
authors.affiliation: College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi 030801, China; Laboratory of Mammalian Reproductive Biology and Genomics, Michigan State University, East Lansing, MI 48824, United States; Department of Animal Science, Michigan State University, East Lansing, MI 48824, United States; Department of Physiology, Michigan State University, East Lansing, MI 48824, United States.
authors.forename: George W
authors.initials: GW
authors.lastname: Smith
authors.affiliation: College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi 030801, China. Electronic address: lihualvsxau@126.com.
authors.forename: Lihua
authors.initials: L
authors.lastname: Lv
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D007985:Leydig Cells / Q000187:drug effects / Q000378:metabolism; D007986:Luteinizing Hormone / Q000378:metabolism / Q000494:pharmacology*; D008297:Male; D008928:Mitochondria / Q000201:enzymology*; D010088:Oxidoreductases / Q000378:metabolism*; D012741:Sexual Maturation; D012756:Sheep / Q000502:physiology*; D013739:Testosterone / Q000378:metabolism*; D014807:Vitamin D / Q000494:pharmacology*
chemical_list: D014807:Vitamin D; D013739:Testosterone; D007986:Luteinizing Hormone; D010088:Oxidoreductases
keywords: 1α,25-(OH)(2)VD(3); LH; Leydig cells; Mitochondrial dehydrogenase activity; Ram; Testosterone