title: High-Resolution Three-Dimensional Computed Tomography Analysis of the Clinical Efficacy of Cultured Autogenous Periosteal Cells in Sinus Lift Bone Grafting.
abstract: OBJECTIVE
  Sinus lift (SL) using cultured autogenous periosteal cells (CAPCs) combined with autogenous bone and platelet-rich plasma (PRP) was performed to evaluate the effect of cell administration on bone regeneration, by using high-resolution three-dimensional computed tomography (CT).
  
  
  METHODS
  SL with autogenous bone and PRP plus CAPC [CAPC(+)SL] was performed in 23 patients. A piece of periosteum taken from the mandible was cultured in M199 medium with 10% fetal bovine serum (FBS) for 6 weeks. As control, 16 patients received SL with autogenous bone and PRP [CAPC(-)SL]. Three-dimensional CT imaging was performed before and 4 months and 1 year after SL, and stratification was performed based on CT numbers (HUs) corresponding to soft tissue and cancellous or cortical bone.
  
  
  RESULTS
  The augmented bone in CAPC(+)SL revealed an increase in HUs corresponding to cancellous bone as well as a decrease in HUs corresponding to grafted cortical bone. In addition, HUs corresponding to cancellous bone in the graft bed were increased in CAPC(+)SL but were decreased in CAPC(-)SL. Insertion torque during implant placement was significantly higher in CAPC(+)SL.
  
  
  CONCLUSIONS
  By promoting bone anabolic activity both in augmented bone and graft bed, CAPCs are expected to aid primary fixation and osseointegration of implants in clinical applications.
authors.affiliation: Oral Implant Clinic, Niigata University Medical and Dental Hospital, Niigata, Japan.
authors.forename: Shin
authors.initials: S
authors.lastname: Ogawa
authors.affiliation: Oral Implant Clinic, Niigata University Medical and Dental Hospital, Niigata, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Hoshina
authors.affiliation: Bioscience Medical Research Center, Niigata University Medical and Dental Hospital, Niigata, Japan.
authors.forename: Koh
authors.initials: K
authors.lastname: Nakata
authors.affiliation: Oral Implant Clinic, Niigata University Medical and Dental Hospital, Niigata, Japan.
authors.forename: Kazuho
authors.initials: K
authors.lastname: Yamada
authors.affiliation: Department of Oral and Maxillofacial Surgery, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
authors.forename: Kohya
authors.initials: K
authors.lastname: Uematsu
authors.affiliation: Division of Dental Pharmacology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Kawase
authors.affiliation: Department of Oral and Maxillofacial Surgery, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
authors.forename: Ritsuo
authors.initials: R
authors.lastname: Takagi
authors.affiliation: Department of Oral and Maxillofacial Surgery, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Nagata
mesh_terms: D000328:Adult; D000368:Aged; D064592:Autografts; D001861:Bone Regeneration; D016025:Bone Transplantation; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D021621:Imaging, Three-Dimensional; D008297:Male; D008437:Maxilla / Q000000981:diagnostic imaging / Q000502:physiology / Q000601:surgery*; D008875:Middle Aged; D010521:Periosteum / Q000166:cytology* / Q000637:transplantation; D053657:Platelet-Rich Plasma; D059546:Sinus Floor Augmentation; D014057:Tomography, X-Ray Computed / Q000379:methods*
keywords: cultured autogenous periosteal cell; sinus lift bone augmentation; three-dimensional computed tomography