title: [Evaluation of biological properties of Pseudomonas aeruginosa strains isolated from patients with sternum and rib osteomyelitis].
abstract: OBJECTIVE
  Study of the role of P. aeruginosa in the development of osteomyelitis of sternum and ribs in cardio-surgery patients, and analysis of the main biological properties of the isolated bacterial strains.
  
  
  METHODS
  132 bacterial cultures were isolated from 83 hospital patients as a result of bacteriological examination during 2007-2013. Wound discharge was the study mate- rial. Sampling, seeding and identification of the isolated cultures was carried out by using the respective test-systems; antibiotic sensitivity was studied by disc-diffusion method.
  
  
  RESULTS
  The proportion of P. aeruginosa was 10.6% (n = 14) that is comparable with data on wound infections of general surgery hospitals. A direct and strong correlation (R = 0.846, p = 0.000132) between hemolytic and phospholipase activity was established during evaluation of virulence properties of the isolated-strains. The degree of film-forming ability varied significantly from 0.122 to 1.412 OD; 64.3% ofthe studied cultures were highly film-forming variants. Statistically significant association between biofilm formation and other studied properties was not found. 4 strains produced VIM2-type metallo-betalactamase and had identical RAPD profiles.
  
  
  CONCLUSIONS
  Considering that earlier the similar cultures were not detected and all of them were isolated at a short interval of time, we have made a conclusion, that their short-term circulation is probably associated with introduction, which was the reason for patient infection. P. aeruginosa could be the etio-pathogen of both early and later complications of cardio-surgical interventions.
authors.forename: A V
authors.initials: AV
authors.lastname: Kasatvo
authors.forename: E S
authors.initials: ES
authors.lastname: Gorovits
authors.forename: M V
authors.initials: MV
authors.lastname: Kuznetsova
authors.forename: O A
authors.initials: OA
authors.lastname: Timasheva
authors.forename: S G
authors.initials: SG
authors.lastname: Sukhanov
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D018441:Biofilms; D006348:Cardiac Surgical Procedures; D052978:Disk Diffusion Antimicrobial Tests; D005122:Exudates and Transudates / Q000382:microbiology; D015870:Gene Expression; D006461:Hemolysis; D006801:Humans; D010019:Osteomyelitis / Q000209:etiology / Q000382:microbiology* / Q000473:pathology; D010740:Phospholipases / Q000235:genetics / Q000378:metabolism; D011183:Postoperative Complications; D011552:Pseudomonas Infections / Q000209:etiology / Q000382:microbiology* / Q000473:pathology; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D012189:Retrospective Studies; D012272:Ribs / Q000382:microbiology* / Q000473:pathology; D012426:Russia; D013249:Sternum / Q000382:microbiology* / Q000473:pathology; D013530:Surgical Wound Infection / Q000209:etiology / Q000382:microbiology* / Q000473:pathology; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D010740:Phospholipases; C425108:beta-lactamase bla(vim-2); D001618:beta-Lactamases