title: Detection of Brucella abortus DNA in aborted goats and sheep in Egypt by real-time PCR.
abstract: BACKGROUND
  Brucellosis is a major zoonoses affects wide range of domesticated as well as wild animals. Despite the eradication program of brucellosis in Egypt, the disease is still endemic among cattle, buffaloes, sheep, goats, and camels.
  
  
  RESULTS
  In the present study, abortion occurred naturally among 25 animals (10 cows, 5 buffaloes, 9 Egyptian Baladi goats and 1 ewe) shared the same pasture were investigated by real-time polymerase chain reaction (RT-PCR). DNA of Brucella (B.) abortus was detected in serum of goats and sheep which has aborted recently by species-specific RT-PCR. The results suggest cross-species infection of B. abortus from cattle to non-preferred hosts raised in close contact.
  
  
  CONCLUSIONS
  This article will renew our knowledge about the Brucella agent causing abortion in small ruminants in Egypt. Information provided in this study is important for surveillance program, because eradication programs and vaccination strategies may have to be adapted accordingly.
authors.affiliation: Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743, Jena, Germany. gamalwareth@hotmail.com.|Institute of Animal Hygiene and Environmental Health, Free University of Berlin, Robert-von-Ostertag Str. 7-13, 14163, Berlin, Germany. gamalwareth@hotmail.com.|Department of Pathology, Faculty of Veterinary Medicine, Benha University, Qalyobia, Egypt. gamalwareth@hotmail.com.
authors.forename: Gamal
authors.initials: G
authors.lastname: Wareth
authors.affiliation: Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743, Jena, Germany. Falk.Melzer@fli.bund.de.
authors.forename: Falk
authors.initials: F
authors.lastname: Melzer
authors.affiliation: Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743, Jena, Germany. Herbert.Tomaso@fli.bund.de.
authors.forename: Herbert
authors.initials: H
authors.lastname: Tomaso
authors.affiliation: Institute of Animal Hygiene and Environmental Health, Free University of Berlin, Robert-von-Ostertag Str. 7-13, 14163, Berlin, Germany. roesler.uwe@vetmed.fu-berlin.de.
authors.forename: Uwe
authors.initials: U
authors.lastname: Roesler
authors.affiliation: Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743, Jena, Germany. Heinrich.Neubauer@fli.bund.de.
authors.forename: Heinrich
authors.initials: H
authors.lastname: Neubauer
mesh_terms: D000034:Abortion, Veterinary / Q000175:diagnosis / Q000382:microbiology*; D000818:Animals; D002003:Brucella abortus / Q000235:genetics* / Q000472:pathogenicity; D002006:Brucellosis / Q000175:diagnosis / Q000382:microbiology / Q000635:transmission / Q000662:veterinary*; D002007:Brucellosis, Bovine / Q000175:diagnosis / Q000382:microbiology / Q000635:transmission; D002020:Buffaloes; D002417:Cattle; D004279:DNA, Viral / Q000235:genetics*; D004534:Egypt; D005260:Female; D015511:Goat Diseases / Q000175:diagnosis / Q000382:microbiology*; D006041:Goats; D011237:Predictive Value of Tests; D011247:Pregnancy; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis / Q000382:microbiology*
chemical_list: D004279:DNA, Viral