title: Spatial variations of bacterial community and its relationship with water chemistry in Sanya Bay, South China Sea as determined by DGGE fingerprinting and multivariate analysis.
abstract: Bacteria play important roles in the structure and function of marine food webs by utilizing nutrients and degrading the pollutants, and their distribution are determined by surrounding water chemistry to a certain extent. It is vital to investigate the bacterial community's structure and identifying the significant factors by controlling the bacterial distribution in the paper. Flow cytometry showed that the total bacterial abundance ranged from 5.27 × 10(5) to 3.77 × 10(6) cells/mL. Molecular fingerprinting technique, denaturing gradient gel electrophoresis (DGGE) followed by DNA sequencing has been employed to investigate the bacterial community composition. The results were then interpreted through multivariate statistical analysis and tended to explain its relationship to the environmental factors. A total of 270 bands at 83 different positions were detected in DGGE profiles and 29 distinct DGGE bands were sequenced. The predominant bacteria were related to Phyla Protebacteria species (31 %, nine sequences), Cyanobacteria (37.9 %, eleven sequences) and Actinobacteria (17.2 %, five sequences). Other phylogenetic groups identified including Firmicutes (6.9 %, two sequences), Bacteroidetes (3.5 %, one sequences) and Verrucomicrobia (3.5 %, one sequences). Conical correspondence analysis was used to elucidate the relationships between the bacterial community compositions and environmental factors. The results showed that the spatial variations in the bacterial community composition was significantly related to phosphate (P = 0.002, P < 0.01), dissolved organic carbon (P = 0.004, P < 0.01), chemical oxygen demand (P = 0.010, P < 0.05) and nitrite (P = 0.016, P < 0.05). This study revealed the spatial variations of bacterial community and significant environmental factors driving the bacterial composition shift. These results may be valuable for further investigation on the functional microbial structure and expression quantitatively under the polluted environments in the world.
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China.|Hainan Tropical Marine Biological Research Station, Sanya, 57200, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Ling
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China.|Hainan Tropical Marine Biological Research Station, Sanya, 57200, China.
authors.forename: Yan-Ying
authors.initials: YY
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China. dongjunde@vip.163.com.|Hainan Tropical Marine Biological Research Station, Sanya, 57200, China. dongjunde@vip.163.com.
authors.forename: Jun-De
authors.initials: JD
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Tropical Oceanography, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China. yswang@scsio.ac.cn.
authors.forename: You-Shao
authors.initials: YS
authors.lastname: Wang
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China.|Hainan Tropical Marine Biological Research Station, Sanya, 57200, China.
authors.forename: Jing-Bing
authors.initials: JB
authors.lastname: Feng
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China.|Hainan Tropical Marine Biological Research Station, Sanya, 57200, China.
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Zhou
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D061006:Bays; D002681:China; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D058645:Denaturing Gradient Gel Electrophoresis; D064307:Microbiota; D008969:Molecular Sequence Data; D015999:Multivariate Analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000378:metabolism; D012623:Seawater / Q000737:chemistry* / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D062206:Spatial Analysis
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: Bacterial community structure; CCA; PCR–DGGE; Sanya Bay; South China Sea; Spatial variations; Water chemistry