title: Cytogenetic and molecular identification of small-segment chromosome translocation lines from wheat-rye substitution lines to create wheat germplasm with beneficial traits.
abstract: Intergeneric crop plant hybrid lines with small-segment chromosome translocations are very useful in plant genetic research and breeding. In this study, to create small-segment chromosome translocations with beneficial agronomic characters, the progeny of wheat-rye substitution lines 5R/5A and 6R/6A were selected from generations F2to F5for rye-specific characteristics. A PCR primer and specific simple sequence repeat marker for rye were used in F5populations to detect rye chromatin and to amplify a specific chromosome band in six translocation lines (06-6-5, 06-6-6, 06-6-9, 6-26-1, 7-23, and 7-33). Fragment pSc119.1 cloned from 7-33 had 99% homology with the big ear gene sequence (GenBank AF512607.1) in wheat. The six lines were further characterized via pollen mother cell meiosis analysis for genetic stability, and chromosome C-banding and genomic in situ hybridization for rye chromatin. The results show that line 7-33 was still within the 5R/5A substitution lines and possessed the big ear gene. The other lines all contained small-segment rye chromosome translocations. The results indicated that substitution line hybridization is an effective method for creating small-segment chromosome translocations with useful agronomic traits. Trials for these six wheat-rye translocation lines are justified because they possess many important stably-inherited agronomic characters, including disease resistance and improved yield.
authors.affiliation: Harbin Normal University, Biology Department , Harbin , P.R. China.
authors.forename: Wei-Fu
authors.initials: WF
authors.lastname: Song
authors.affiliation: Daqing Normal University, School of Life Science, Daqing , Heilongjiang , P.R. China.
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Ding
authors.affiliation: Harbin Normal University, Biology Department , Harbin , P.R. China.
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Zhang
authors.affiliation: Harbin Normal University, Biology Department , Harbin , P.R. China.
authors.forename: Ji-Lin
authors.initials: JL
authors.lastname: Li
authors.affiliation: Heilongjiang Academy of Agricultural Sciences, Crop Breeding Institute , Harbin , P.R. China.
authors.forename: Zhi-Min
authors.initials: ZM
authors.lastname: Xiao
authors.affiliation: Heilongjiang Academy of Agricultural Sciences, Crop Breeding Institute , Harbin , P.R. China.
authors.forename: Wen-Li
authors.initials: WL
authors.lastname: Xin
authors.affiliation: Heilongjiang Academy of Agricultural Sciences, Crop Breeding Institute , Harbin , P.R. China.
authors.forename: Qing-Jie
authors.initials: QJ
authors.lastname: Song
authors.affiliation: Heilongjiang Academy of Agricultural Sciences, Crop Breeding Institute , Harbin , P.R. China.
authors.forename: Hai-Bin
authors.initials: HB
authors.lastname: Zhao
authors.affiliation: Heilongjiang Academy of Agricultural Sciences, Crop Breeding Institute , Harbin , P.R. China.
authors.forename: Yan-Bin
authors.initials: YB
authors.lastname: Zhang
authors.affiliation: Heilongjiang Academy of Agricultural Sciences, Crop Breeding Institute , Harbin , P.R. China.
authors.forename: Chun-Li
authors.initials: CL
authors.lastname: Zhang
keywords: C-banding; genomic in situ hybridization; substitution line hybridization; wheat variety trials