title: Chromosome Conformation Capture Carbon Copy (5C) in Budding Yeast.
abstract: Chromosome conformation capture carbon copy (5C) is a high-throughput method for detecting ligation products of interest in a chromosome conformation capture (3C) library. 5C uses ligation-mediated amplification (LMA) to generate carbon copies of 3C ligation product junctions using single-stranded oligonucleotide probes. This procedure produces a 5C library of short DNA molecules which represent the interactions between the corresponding restriction fragments. The 5C library can be amplified using universal primers containing the Illumina paired-end adaptor sequences for subsequent high-throughput sequencing.
authors.affiliation: Program in Systems Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01605.
authors.forename: Jon-Matthew
authors.initials: JM
authors.lastname: Belton
authors.affiliation: Program in Systems Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01605.
authors.forename: Job
authors.initials: J
authors.lastname: Dekker
mesh_terms: D002244:Carbon; D002843:Chromatin; D002874:Chromosome Mapping / Q000379:methods; D002875:Chromosomes / Q000378:metabolism*; D017931:DNA Primers / Q000378:metabolism; D015723:Gene Library; D009690:Nucleic Acid Conformation; D004718:Saccharomycetales / Q000502:physiology* / Q000648:ultrastructure*
chemical_list: D002843:Chromatin; D017931:DNA Primers; D002244:Carbon