title: [Biosynthesis of isocitric acid by the yeast yarrowia lipolytica and its regulation].
abstract: We studied the biosynthesis of isocitric acid from rapeseed (canola) oil by the yeast Yarrowia lipolytica and its regulation. We determined a fundamental possibility for directed biosynthesis of isocitric acid by Y lipolytica yeast, with only minimal amounts of citric acid byproduct, when grown on a medium containing canola oil. Wild type strains of Y lipolytica were mutagenized by UV irradiation and treatment with N-methyl-N'-nitro-N-nitrosoguanidine (NG). Subsequent selection on media with acetate and isocitrate resulted in isolation of a UV/NG Y lipolytica UV/NG mutant that synthesized isocitrate and citrate at a ratio of 2.7:1. In the parent strain, this ratio is 1:1. Inhibition of isocitrate lyase, a key enzyme in the metabolism of isocitric acid, by the addition of itaconic acid resulted in increased synthesis of isocitrate with a ratio of isocitrate to citrate reaching 6:1. Culturing of the Y lipolytica UV/NG mutant in a pilot industrial fermenter in the presence of itaconic acid resulted in the production of 88.7 g/L of isocitric acid with a yield of 90%.
authors.forename: S V
authors.initials: SV
authors.lastname: Kamzolova
authors.forename: Y N
authors.initials: YN
authors.lastname: Lunin
authors.forename: R K
authors.initials: RK
authors.lastname: Allayarov
authors.forename: I F
authors.initials: IF
authors.lastname: Puntus
authors.forename: I A
authors.initials: IA
authors.lastname: Laptev
authors.forename: V A
authors.initials: VA
authors.lastname: Samojlenko
authors.forename: I G
authors.initials: IG
authors.lastname: Morgunov
mesh_terms: D003470:Culture Media / Q000737:chemistry; D005229:Fatty Acids, Monounsaturated / Q000378:metabolism; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods*; D007522:Isocitrate Lyase / Q000037:antagonists & inhibitors / Q000378:metabolism; D007523:Isocitrates / Q000378:metabolism*; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D016296:Mutagenesis; D010938:Plant Oils / Q000378:metabolism; D000074262:Rapeseed Oil; D013386:Succinates / Q000494:pharmacology; D014466:Ultraviolet Rays; D025062:Yarrowia / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects
chemical_list: D003470:Culture Media; D005229:Fatty Acids, Monounsaturated; D007523:Isocitrates; D010938:Plant Oils; D000074262:Rapeseed Oil; D013386:Succinates; D008769:Methylnitronitrosoguanidine; C034219:isocitric acid; D007522:Isocitrate Lyase; C005229:itaconic acid