title: Attenuation of Colitis by Serum-Derived Bovine Immunoglobulin/Protein Isolate in a Defined Microbiota Mouse Model.
abstract: BACKGROUND
  The pathogenesis of inflammatory bowel disease (IBD) is complex and multifaceted including genetic predisposition, environmental components, microbial dysbiosis, and inappropriate immune activation to microbial components. Pathogenic bacterial provocateurs like adherent and invasive E. coli have been reported to increase susceptibility to Crohn's disease. Serum-derived bovine immunoglobulin/protein isolate (SBI) is comprised primarily of immunoglobulins (Igs) that bind to conserved microbial components and neutralize exotoxins.
  
  
  OBJECTIVE
  To demonstrate that oral administration of SBI may modulate mucosal inflammation following colonization with E. coli, LF82, and exposure to dextran sodium sulfate (DSS).
  
  
  METHODS
  Defined microbiota mice harboring the altered Schaedler flora (ASF) were administered SBI or hydrolyzed collagen twice daily starting 7 days prior to challenge with E. coli LF82 and continuing for the remainder of the experiment. Mice were treated with DSS for 7 days and then evaluated for evidence of local and peripheral inflammation.
  
  
  RESULTS
  Igs within SBI bound multiple antigens from all eight members of the ASF and E. coli LF82 by western blot analysis. Multiple parameters of LF82/DSS-induced colitis were reduced following administration of SBI, including histological lesion scores, secretion of cytokines and chemokines from cecal biopsies, intestinal fatty acid binding protein (I-FABP) and serum amyloid A from plasma.
  
  
  CONCLUSIONS
  Oral administration of SBI attenuated clinical signs of LF82/DSS-induced colitis in mice. The data are consistent with the hypothesis that SBI immunoglobulin binding of bacterial antigens in the intestinal lumen may inhibit the inflammatory cascades that contribute to IBD, thus attenuating DSS-induced colitis.
authors.affiliation: Entera Health, Inc., 2575 SE Oak Tree Ct., Ankeny, IA, 50023, USA. Abigail.henderson@enterahealth.com.
authors.forename: Abigail L
authors.initials: AL
authors.lastname: Henderson
authors.affiliation: Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, 50011, USA.
authors.forename: Meghan Wymore
authors.initials: MW
authors.lastname: Brand
authors.affiliation: Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, 50011, USA.
authors.forename: Ross J
authors.initials: RJ
authors.lastname: Darling
authors.affiliation: Entera Health, Inc., 2575 SE Oak Tree Ct., Ankeny, IA, 50023, USA.|Entera Health, Inc., Cary, NC, 27518, USA.
authors.forename: Kenneth J
authors.initials: KJ
authors.lastname: Maas
authors.affiliation: Entera Health, Inc., 2575 SE Oak Tree Ct., Ankeny, IA, 50023, USA.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Detzel
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA, 50011, USA.
authors.forename: Jesse
authors.initials: J
authors.lastname: Hostetter
authors.affiliation: Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, 50011, USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Wannemuehler
authors.affiliation: Entera Health, Inc., 2575 SE Oak Tree Ct., Ankeny, IA, 50023, USA.
authors.forename: Eric M
authors.initials: EM
authors.lastname: Weaver
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001419:Bacteria / Q000145:classification / Q000254:growth & development / Q000276:immunology*; D018925:Chemokines / Q000378:metabolism; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D003106:Colon / Q000187:drug effects* / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D016207:Cytokines / Q000378:metabolism; D016264:Dextran Sulfate; D004195:Disease Models, Animal; D004926:Escherichia coli; D005260:Female; D005856:Germ-Free Life; D007136:Immunoglobulins / Q000008:administration & dosage / Q000494:pharmacology*; D007422:Intestines / Q000382:microbiology*; D008297:Male; D008809:Mice, Inbred C3H; D064307:Microbiota
chemical_list: D000942:Antigens, Bacterial; D018925:Chemokines; D016207:Cytokines; D007136:Immunoglobulins; D016264:Dextran Sulfate
keywords: Bovine immunoglobulin; Dextran sulfate sodium (DSS); Inflammatory bowel disease (IBD); Microbiota; Serum