title: Reverse Correlation between MicroRNA-145 and FSCN1 Affecting Gastric Cancer Migration and Invasion.
abstract: MicroRNAs (miRs) play important roles in modulating gene expression during the processes of tumorigenesis and tumor development. Previous studies have found that miR-145 is down-regulated in the stomach neoplasm and is related to tumor migration and invasion. However, both the molecular mechanism and function of miR-145 in gastric cancer remain unclear. The present study is the first demonstration of the significant down-regulation of miR-145 expression in infiltrative gastric cancer compared to expanding gastric cancer. Additionally, correlation analyses revealed strong inverse correlations between miR-145 and FSCN1 expression levels in infiltrative gastric cancer. Furthermore, we demonstrated that miR-145 directly targets FSCN1 and suppresses cell migration and invasion in gastric cancer. Knocking down the expression of FSCN1 led to the suppression of migration and invasion in gastric cancer cells, and re-expressing FSCN1 in miR-145-overexpressing cells reversed their migration and invasion defects. Thus, we concluded that miR-145 regulates cell migration and invasion in gastric cancer primarily by directly targeting FSCN1.
authors.affiliation: Department of Gastrointestinal Surgery, Zhongshan Hospital of Xiamen University, Xiamen, Fujian, China; Institute of Gastrointestinal Oncology, Medical College of Xiamen University, Xiamen, Fujian, China.
authors.forename: Jia-jia
authors.initials: JJ
authors.lastname: Chen
authors.affiliation: Department of Gastrointestinal Surgery, Zhongshan Hospital of Xiamen University, Xiamen, Fujian, China; Institute of Gastrointestinal Oncology, Medical College of Xiamen University, Xiamen, Fujian, China.
authors.forename: Wang-yu
authors.initials: WY
authors.lastname: Cai
authors.affiliation: Department of Gastrointestinal Surgery, Zhongshan Hospital of Xiamen University, Xiamen, Fujian, China; Institute of Gastrointestinal Oncology, Medical College of Xiamen University, Xiamen, Fujian, China.
authors.forename: Xue-wen
authors.initials: XW
authors.lastname: Liu
authors.affiliation: Department of Gastrointestinal Surgery, Zhongshan Hospital of Xiamen University, Xiamen, Fujian, China; Institute of Gastrointestinal Oncology, Medical College of Xiamen University, Xiamen, Fujian, China.
authors.forename: Qi-cong
authors.initials: QC
authors.lastname: Luo
authors.affiliation: Department of Gastrointestinal Surgery, Zhongshan Hospital of Xiamen University, Xiamen, Fujian, China; Institute of Gastrointestinal Oncology, Medical College of Xiamen University, Xiamen, Fujian, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.affiliation: Department of Gastrointestinal Surgery, Zhongshan Hospital of Xiamen University, Xiamen, Fujian, China.
authors.forename: Wei-feng
authors.initials: WF
authors.lastname: Huang
authors.affiliation: Department of Gastrointestinal Surgery, Zhongshan Hospital of Xiamen University, Xiamen, Fujian, China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: Department of Gastrointestinal Surgery, Zhongshan Hospital of Xiamen University, Xiamen, Fujian, China; Institute of Gastrointestinal Oncology, Medical College of Xiamen University, Xiamen, Fujian, China.
authors.forename: Jian-chun
authors.initials: JC
authors.lastname: Cai
mesh_terms: D020413:3' Untranslated Regions; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D057809:HEK293 Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D034741:RNA, Small Interfering / Q000378:metabolism; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology*
chemical_list: D020413:3' Untranslated Regions; D002352:Carrier Proteins; C502658:FSCN1 protein, human; C541010:MIRN145 microRNA, human; D035683:MicroRNAs; D008840:Microfilament Proteins; D034741:RNA, Small Interfering