title: The immunohistochemical peptidergic expression of leptin is associated with recurrence of malignancy in laryngeal squamous cell carcinoma.
abstract: Leptin is a peptide that plays a key role in the control of satiety, energy expenditure, food intake and various reproductive processes. In the last years, the expression of leptin had been found in malignant cells of various origins. The aim of this study is to evaluate leptin expression in human laryngeal squamous cell carcinoma (SCC) and to investigate its possible role in predicting prognosis. Leptin expression was determined by immunohistochemistry in pathological and healthy tissue specimens from 24 patients with laryngeal SCC. Specimens were stained with an anti-leptin antibody. All measurements were performed using a computer-based image analysis system and scale of staining intensity was determined. All tumoural specimens showed significant immunoreactivity for leptin compared to healthy tissues (p ≤ 0.05), but showed different immunoreactivity that was related to clinicopathological features. High leptin expression was not significantly related with TNM, histological grading (HG) or advanced (III and IV) clinical stage (p > 0.05). Recurrence of malignancy was found to be significantly related with high expression of leptin by Spearman's rank correlation test (r = 0.59; p = 0.002), Fisher's test (p = 0.017) and Kaplan- Meier product-limit estimate (Log-rank test, p ≤ 0.05). In particular, multivariate logistic regression analysis showed that recurrences were significantly related with nodal involvement, HG and leptin expression (p ≤ 0.05). These preliminary results suggest that leptin may be a valuable parameter for predicting prognosis in laryngeal SCC.
authors.affiliation: Otorhinolaryngology Section, Department of Experimental Biomedicine and Clinical Neurosciences, (BioNeC), University of Palermo;
authors.forename: S
authors.initials: S
authors.lastname: Gallina
authors.affiliation: Otorhinolaryngology Section, Department of Experimental Biomedicine and Clinical Neurosciences, (BioNeC), University of Palermo;
authors.forename: F
authors.initials: F
authors.lastname: Sireci
authors.affiliation: Otorhinolaryngology Section, Department of Experimental Biomedicine and Clinical Neurosciences, (BioNeC), University of Palermo;
authors.forename: F
authors.initials: F
authors.lastname: Lorusso
authors.affiliation: Otorhinolaryngology Section, Department of Experimental Biomedicine and Clinical Neurosciences, (BioNeC), University of Palermo;
authors.forename: D V
authors.initials: DV
authors.lastname: DI Benedetto
authors.affiliation: Otorhinolaryngology Section, Department of Experimental Biomedicine and Clinical Neurosciences, (BioNeC), University of Palermo;
authors.forename: R
authors.initials: R
authors.lastname: Speciale
authors.affiliation: Otorhinolaryngology Section, Department of Experimental Biomedicine and Clinical Neurosciences, (BioNeC), University of Palermo;
authors.forename: D
authors.initials: D
authors.lastname: Marchese
authors.affiliation: Department of Science for Health Promotion and Mother to Child Care "G. D'Alessandro", University of Palermo;
authors.forename: C
authors.initials: C
authors.lastname: Costantino
authors.affiliation: Department of Science for Health Promotion and Mother to Child Care "G. D'Alessandro", University of Palermo;
authors.forename: G
authors.initials: G
authors.lastname: Napoli
authors.affiliation: Histology and Embryology Section, Department of Experimental Biomedicine and Clinical Neurosciences, (BioNeC), University of Palermo.
authors.forename: V
authors.initials: V
authors.lastname: Tessitore
authors.affiliation: Histology and Embryology Section, Department of Experimental Biomedicine and Clinical Neurosciences, (BioNeC), University of Palermo.
authors.forename: D
authors.initials: D
authors.lastname: Cucco
authors.affiliation: Histology and Embryology Section, Department of Experimental Biomedicine and Clinical Neurosciences, (BioNeC), University of Palermo.
authors.forename: A
authors.initials: A
authors.lastname: Leone
authors.affiliation: Histology and Embryology Section, Department of Experimental Biomedicine and Clinical Neurosciences, (BioNeC), University of Palermo.
authors.forename: G
authors.initials: G
authors.lastname: Bonaventura
authors.affiliation: Histology and Embryology Section, Department of Experimental Biomedicine and Clinical Neurosciences, (BioNeC), University of Palermo.
authors.forename: M L
authors.initials: ML
authors.lastname: Uzzo
authors.affiliation: Histology and Embryology Section, Department of Experimental Biomedicine and Clinical Neurosciences, (BioNeC), University of Palermo.
authors.forename: G F
authors.initials: GF
authors.lastname: Spatola
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D007822:Laryngeal Neoplasms / Q000737:chemistry / Q000378:metabolism*; D020738:Leptin / Q000032:analysis / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000453:epidemiology*; D009479:Neuropeptides / Q000502:physiology; D011237:Predictive Value of Tests; D011379:Prognosis
chemical_list: D020738:Leptin; D009479:Neuropeptides
keywords: Laryngeal carcinoma; Leptin; Malignancy recurrence