title: Microwave-Assisted Resolution of α-Lipoic Acid Catalyzed by an Ionic Liquid Co-Lyophilized Lipase.
abstract: The combination of the ionic liquid co-lyophilized lipase and microwave irradiation was used to improve enzyme performance in enantioselective esterification of α-lipoic acid. Effects of various reaction conditions on enzyme activity and enantioselectivity were investigated. Under optimal condition, the highest enantioselectivity (E = 41.2) was observed with a high enzyme activity (178.1 μmol/h/mg) when using the ionic liquid co-lyophilized lipase with microwave assistance. Furthermore, the ionic liquid co-lyophilized lipase exhibited excellent reusability under low power microwave.
authors.affiliation: Central Laboratory, The Second Hospital of Jilin University, Changchun 130021, China. liu_ning@jlu.edu.cn.
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
authors.affiliation: Key Laboratory of Molecular Enzymology and Engineering of Ministry of Education, College of Life Science, Jilin University, Changchun 130012, China. w_lei@jlu.edu.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Key Laboratory of Molecular Enzymology and Engineering of Ministry of Education, College of Life Science, Jilin University, Changchun 130012, China. wangzhi@jlu.edu.cn.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Key Laboratory of Molecular Enzymology and Engineering of Ministry of Education, College of Life Science, Jilin University, Changchun 130012, China. jiangliyan@jlu.edu.cn.
authors.forename: Liyan
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Molecular Enzymology and Engineering of Ministry of Education, College of Life Science, Jilin University, Changchun 130012, China. wangzhi@jlu.edu.cn.
authors.forename: Zhuofu
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Key Laboratory of Molecular Enzymology and Engineering of Ministry of Education, College of Life Science, Jilin University, Changchun 130012, China. xinqingtianlan@sina.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Yue
authors.affiliation: The First Hospital of Jilin University, Changchun 130021, China. xiexn@jlu.edu.cn.
authors.forename: Xiaona
authors.initials: X
authors.lastname: Xie
mesh_terms: D000818:Animals; D002175:Candida / Q000737:chemistry / Q000201:enzymology; D002384:Catalysis; D057075:Enzyme Assays; D017743:Equipment Reuse; D004951:Esterification; D005612:Freeze Drying; D007093:Imidazoles / Q000737:chemistry*; D052578:Ionic Liquids / Q000737:chemistry*; D007700:Kinetics; D008049:Lipase / Q000737:chemistry*; D008872:Microwaves; D009089:Mucor / Q000737:chemistry / Q000201:enzymology; D012997:Solvents / Q000737:chemistry; D013237:Stereoisomerism; D013552:Swine; D008063:Thioctic Acid / Q000737:chemistry*
chemical_list: C419324:1-butyl-3-methylimidazolium tetrafluoroborate; D007093:Imidazoles; D052578:Ionic Liquids; D012997:Solvents; D008063:Thioctic Acid; D008049:Lipase
keywords: enantioselectivity; enzyme activity; esterification; ionic liquid co-lyophilized lipase; microwave; α-lipoic acid