title: Chronic SIV and morphine treatment increases heat shock protein 5 expression at the synapse.
abstract: The abuse of opiates such as morphine in synergy with HIV infection accelerates neurocognitive impairments and neuropathology in the CNS of HIV-infected subjects, collectively referred to as HAND. To identify potential pathogenic markers associated with HIV and morphine in perturbing the synaptic architecture, we performed quantitative mass spectrometry proteomics on purified synaptosomes isolated from the caudate of two groups of rhesus macaques chronically infected with SIV differing by one regimen-morphine treatment. The upregulation of heat shock 70-kDa protein 5 in the SIV + morphine group points to increased cellular stress during SIV/morphine interaction thus leading to CNS dysfunction.
authors.affiliation: Department of Anesthesiology, University of Nebraska Medical Center, Omaha, NE, USA.|Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, 985880 Nebraska Medical Center, Omaha, NE, 68198, USA.
authors.forename: Gurudutt
authors.initials: G
authors.lastname: Pendyala
authors.affiliation: Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, 985880 Nebraska Medical Center, Omaha, NE, 68198, USA.
authors.forename: Palsamy
authors.initials: P
authors.lastname: Periyasamy
authors.affiliation: Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, 985880 Nebraska Medical Center, Omaha, NE, 68198, USA.
authors.forename: Shannon
authors.initials: S
authors.lastname: Callen
authors.affiliation: Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, 985880 Nebraska Medical Center, Omaha, NE, 68198, USA.
authors.forename: Howard S
authors.initials: HS
authors.lastname: Fox
authors.affiliation: Department of Anesthesiology, University of Nebraska Medical Center, Omaha, NE, USA.
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Lisco
authors.affiliation: Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, 985880 Nebraska Medical Center, Omaha, NE, 68198, USA. sbuch@unmc.edu.
authors.forename: Shilpa J
authors.initials: SJ
authors.lastname: Buch
mesh_terms: D000701:Analgesics, Opioid / Q000633:toxicity*; D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D000091342:Endoplasmic Reticulum Chaperone BiP; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006801:Humans; D008253:Macaca mulatta; D009020:Morphine / Q000633:toxicity*; D009474:Neurons / Q000187:drug effects; D040901:Proteomics / Q000379:methods; D060888:Real-Time Polymerase Chain Reaction; D016097:Simian Acquired Immunodeficiency Syndrome / Q000378:metabolism / Q000473:pathology*; D015302:Simian Immunodeficiency Virus; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013569:Synapses / Q000187:drug effects / Q000378:metabolism*; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D000701:Analgesics, Opioid; D000091342:Endoplasmic Reticulum Chaperone BiP; C000717247:HSPA5 protein, human; D006360:Heat-Shock Proteins; D009020:Morphine
keywords: CNS; HSPA5; Morphine; Proteomics; SIV; Synaptosomes