title: Synthesis of poly(sulfobetaine methacrylate)-grafted chitosan under γ-ray irradiation for alamethicin assembly.
abstract: Interaction between peptide and lipid membrane plays a major role in biological activity of membrane-active peptide. We describe here a new biocompatible polymeric assembly to support membrane peptide. Specifically, chitosan-graft-poly(sulfobetaine methacrylate) (CS-g-PSBMA) was synthesized for alamethicin assembly by controlled polymerization under γ-ray irradiation. The graft copolymer could self-assemble into micelles in distilled water for supporting alamethicin. The assembly of alamethicin with CS-g-PSBMA micelles in aqueous solutions was related with the ratio of alamethicin/CS-g-PSBMA: the more alamethicin, the smaller sizes of the hybrid complex. Moreover, alamethicin penetrated into the hydrophobic cores of CS-g-PSBMA micelles while displayed secondary helical conformation in the complex. The results indicate that CS-g-PSBMA assemblies can be used to support membrane peptide.
authors.affiliation: School for Radiological and Interdisciplinary Sciences (RAD-X) & College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China. Electronic address: yy2367cy@163.com.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: School for Radiological and Interdisciplinary Sciences (RAD-X) & College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China. Electronic address: dongping8688@163.com.
authors.forename: Ping
authors.initials: P
authors.lastname: Dong
authors.affiliation: School for Radiological and Interdisciplinary Sciences (RAD-X) & College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China. Electronic address: wyqnt@163.com.
authors.forename: Yanqi
authors.initials: Y
authors.lastname: Wei
authors.affiliation: School for Radiological and Interdisciplinary Sciences (RAD-X) & College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China. Electronic address: qianjun_1116@163.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Qian
authors.affiliation: School for Radiological and Interdisciplinary Sciences (RAD-X) & College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China; Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Suzhou 215123, China. Electronic address: dbhua_lab@suda.edu.cn.
authors.forename: Daoben
authors.initials: D
authors.lastname: Hua
mesh_terms: D000408:Alamethicin / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D005720:Gamma Rays; D008689:Methacrylates / Q000737:chemistry*; D008823:Micelles
chemical_list: D008689:Methacrylates; D008823:Micelles; C521589:sulfobetaine methacrylate polymer; D000408:Alamethicin; D048271:Chitosan
keywords: Alamethicin; Biocompatibility; Chitosan; Self-assembly; Sulfobetaine