title: Evaluation of single nucleotide polymorphisms in chromosomal regions impacting pregnancy status in cattle.
abstract: Reproductive success is an important component of commercial beef cattle production, and identification of DNA markers with predictive merit for reproductive success would facilitate accurate prediction of mean daughter pregnancy rate, enabling effective selection of bulls to improve female fertility. A previous study identified SNP associated with beef cattle reproductive efficiency based on a genomewide association analysis approach using genotyping multiple-animal pools of DNA to increase the number of animals that could be genotyped with available resources. For the current study, we expand on this previous study by individually genotyping cattle from the pooling study for 89 SNP that were associated with female pregnancy rate. The aims of the study were to confirm the results of the pooling study and, more specifically, identify modes of gene action and DNA variations such as haplotypes that would not be possible with pooled genotyping. Eighty-nine SNP selected from the pooling study were evaluated using the Sequenom MassARRAY system to individually genotype animals from populations evaluated in the pooling study, including both and breeds. From this research, regions on chromosomes 5 (26.3-48.1 Mb; UMD3.1 assembly) and 9 (37,436,575 bp; UMD3.1 assembly), first identified in the previous pooling study, were shown through individual genotyping to harbor genetic variation ( < 0.05 genomewide significance) affecting reproductive efficiency in interspecific crosses ( and ) of cattle. Each of these markers exhibited additive (vs. dominant) gene action. Additionally, a haplotype block harboring an allele of origin with negative effects on reproduction was identified on chromosome 5 in interspecific composite breeds of × composites.
authors.forename: K M
authors.initials: KM
authors.lastname: Psaros
authors.forename: T G
authors.initials: TG
authors.lastname: McDaneld
authors.forename: L A
authors.initials: LA
authors.lastname: Kuehn
authors.forename: W M
authors.initials: WM
authors.lastname: Snelling
authors.forename: J W
authors.initials: JW
authors.lastname: Keele
mesh_terms: D000483:Alleles; D000818:Animals; D001947:Breeding; D002417:Cattle / Q000235:genetics* / Q000502:physiology*; D005260:Female; D005819:Genetic Markers; D014644:Genetic Variation; D005838:Genotype; D006239:Haplotypes; D008297:Male; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D018873:Pregnancy Rate; D012098:Reproduction / Q000235:genetics
chemical_list: D005819:Genetic Markers