title: Preparation and characterization of a bacterial cellulose/silk fibroin sponge scaffold for tissue regeneration.
abstract: Bacterial cellulose (BC) and silk fibroin (SF) are natural biopolymers successfully applied in tissue engineering and biomedical fields. In this work nanocomposites based on BC and SF were prepared and characterized by scanning electron microscopy (SEM), infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and thermogravimetric analysis (TGA). In addition, the investigation of cytocompatibility was done by MTT, XTT and Trypan Blue dye technique. Cellular adhesion and proliferation were detected additionally. The evaluation of genotoxicity was realized by micronucleus assay. In vitro tests showed that the material is non-cytotoxic or genotoxic. SEM images revealed a greater number of cells attached at the BC/SF:50% scaffold surface than the pure BC one, suggesting that the presence of fibroin improved cell attachment. This could be related to the SF amino acid sequence that acts as cell receptors facilitating cell adhesion and growth. Consequently, BC/SF:50% scaffolds configured an excellent option in bioengineering depicting its potential for tissue regeneration and cultivation of cells on nanocomposites.
authors.affiliation: School of Dentistry/Unesp, São Paulo State University - Unesp, Rua Humaitá, 1680, Zip code 14801-903, Araraquara, SP, Brazil(2).
authors.forename: H G
authors.initials: HG
authors.lastname: Oliveira Barud
authors.affiliation: Institute of Chemistry - São Paulo State University - Unesp, P.O. Box 355, Araraquara, SP 14801-970, Brazil(1); Laboratório de Química Medicinal e Medicina Regenerativa (QUIMMERA) - Centro Universitário de Araraquara (UNIARA), Araraquara, SP, Brazil. Electronic address: hernane.barud@gmail.com.
authors.forename: Hernane da S
authors.initials: Hda S
authors.lastname: Barud
authors.affiliation: Institute of Chemistry - São Paulo State University - Unesp, P.O. Box 355, Araraquara, SP 14801-970, Brazil(1).
authors.forename: Maurício
authors.initials: M
authors.lastname: Cavicchioli
authors.affiliation: Institute of Chemistry - São Paulo State University - Unesp, P.O. Box 355, Araraquara, SP 14801-970, Brazil(1).
authors.forename: Thais Silva
authors.initials: TS
authors.lastname: do Amaral
authors.affiliation: School of Dentistry/Unesp, São Paulo State University - Unesp, Rua Humaitá, 1680, Zip code 14801-903, Araraquara, SP, Brazil(2).
authors.forename: Osmir Batista
authors.initials: OB
authors.lastname: de Oliveira Junior
authors.affiliation: Gonçalo Moniz Research Center, FIOCRUZ, Av. Waldemar Falcão, 121, Zip code 40296-710, Salvador, BA, Brazil(3).
authors.forename: Diego M
authors.initials: DM
authors.lastname: Santos
authors.affiliation: Gonçalo Moniz Research Center, FIOCRUZ, Av. Waldemar Falcão, 121, Zip code 40296-710, Salvador, BA, Brazil(3).
authors.forename: Antonio Luis de Oliveira Almeida
authors.initials: AL
authors.lastname: Petersen
authors.affiliation: Gonçalo Moniz Research Center, FIOCRUZ, Av. Waldemar Falcão, 121, Zip code 40296-710, Salvador, BA, Brazil(3).
authors.forename: Fabiana
authors.initials: F
authors.lastname: Celes
authors.affiliation: Gonçalo Moniz Research Center, FIOCRUZ, Av. Waldemar Falcão, 121, Zip code 40296-710, Salvador, BA, Brazil(3).
authors.forename: Valéria Matos
authors.initials: VM
authors.lastname: Borges
authors.affiliation: Gonçalo Moniz Research Center, FIOCRUZ, Av. Waldemar Falcão, 121, Zip code 40296-710, Salvador, BA, Brazil(3).
authors.forename: Camila I
authors.initials: CI
authors.lastname: de Oliveira
authors.affiliation: University of Franca, Av. Dr. Armando Salles de Oliveira, 201, Zip code 14404-600, Franca, SP, Brazil(4).
authors.forename: Pollyanna Francielli
authors.initials: PF
authors.lastname: de Oliveira
authors.affiliation: University of Franca, Av. Dr. Armando Salles de Oliveira, 201, Zip code 14404-600, Franca, SP, Brazil(4).
authors.forename: Ricardo Andrade
authors.initials: RA
authors.lastname: Furtado
authors.affiliation: University of Franca, Av. Dr. Armando Salles de Oliveira, 201, Zip code 14404-600, Franca, SP, Brazil(4).
authors.forename: Denise Crispim
authors.initials: DC
authors.lastname: Tavares
authors.affiliation: Institute of Chemistry - São Paulo State University - Unesp, P.O. Box 355, Araraquara, SP 14801-970, Brazil(1).
authors.forename: Sidney J L
authors.initials: SJ
authors.lastname: Ribeiro
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D002482:Cellulose / Q000737:chemistry*; D003412:Cricetulus; D005349:Fibroins / Q000737:chemistry*; D041821:Gluconacetobacter; D051379:Mice; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D012995:Solubility; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds
chemical_list: D002482:Cellulose; D005349:Fibroins
keywords: Bacterial cellulose; Biocompatible materials; MTT (PubChem CID: 64965); Nanocomposites; Scaffold; Silk fibroin; Tissue engineering.; XTT (PubChem CID: 14195569); d-Glucose (PubChem CID: 5793); glycine (PubChem CID: 750); l-Alanine (PubChem CID: 5950)