title: P2X7 Receptor Expression in Peripheral Blood Monocytes Is Correlated With Plasma C-Reactive Protein and Cytokine Levels in Patients With Type 2 Diabetes Mellitus: a Preliminary Report.
abstract: Chronic inflammation plays a major role in development of type 2 diabetes mellitus (T2DM). C-reactive protein (CRP) and inflammatory cytokines such as tumor necrosis factor-α (TNF-α) and interleukin 1β (IL-1β) are directly involved in the occurrence of insulin resistance. Increased extracellular ATP levels can amplify the inflammatory response in vivo via the P2X7 receptor. The present study aimed to assess the relationship between P2X7 receptor expression in human peripheral blood monocytes and plasma levels of TNF-α, IL-1β, and CRP in T2DM patients. The results showed the association of increased P2X7 receptor expression of monocytes with high serum CRP, TNF-α, and IL-1β levels. TNF-α and IL-1β levels were lowest in healthy subjects; in T2DM patients, these inflammatory markers were less abundant in individuals with normal CRP levels compared to those with high CRP contents. In contrast, IL-10 levels in T2DM patients with high CRP levels were dramatically decreased. P2X7 receptor expression in monocytes from T2DM patients with high CRP levels was significantly increased in comparison with healthy individuals and T2DM patients with normal CRP levels. These findings indicated that P2X7 receptor in peripheral blood monocytes may be involved in the pathological changes of T2DM, particularly affecting patients with high CRP levels.
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi, 330006, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wu
authors.affiliation: First Affiliated Hospital, Medical School of Nanchang University, Nanchang, Jiangxi, 330006, People's Republic of China.
authors.forename: Yijun
authors.initials: Y
authors.lastname: Nie
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi, 330006, People's Republic of China.
authors.forename: Huangui
authors.initials: H
authors.lastname: Xiong
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi, 330006, People's Republic of China.
authors.forename: Shuangmei
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi, 330006, People's Republic of China.
authors.forename: Guilin
authors.initials: G
authors.lastname: Li
authors.affiliation: Jiangxi University of Finance and Economics, Nanchang, Jiangxi, 330006, People's Republic of China.
authors.forename: An
authors.initials: A
authors.lastname: Huang
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi, 330006, People's Republic of China.
authors.forename: Lili
authors.initials: L
authors.lastname: Guo
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi, 330006, People's Republic of China.
authors.forename: Shouyu
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi, 330006, People's Republic of China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Xue
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi, 330006, People's Republic of China.
authors.forename: Bing
authors.initials: B
authors.lastname: Wu
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi, 330006, People's Republic of China.
authors.forename: Lichao
authors.initials: L
authors.lastname: Peng
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi, 330006, People's Republic of China.
authors.forename: Miaomiao
authors.initials: M
authors.lastname: Song
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi, 330006, People's Republic of China.
authors.forename: Guodong
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi, 330006, People's Republic of China. liangsd@hotmail.com.
authors.forename: Shangdong
authors.initials: S
authors.lastname: Liang
mesh_terms: D000328:Adult; D002097:C-Reactive Protein / Q000032:analysis*; D016022:Case-Control Studies; D016207:Cytokines / Q000097:blood*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000175:diagnosis; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000097:blood*; D053583:Interleukin-1beta / Q000097:blood; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D058486:Receptors, Purinergic P2X7 / Q000097:blood*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D016207:Cytokines; C508585:IL1B protein, human; D018836:Inflammation Mediators; D053583:Interleukin-1beta; C546468:P2RX7 protein, human; D058486:Receptors, Purinergic P2X7; D014409:Tumor Necrosis Factor-alpha; D002097:C-Reactive Protein
keywords: P2X7 receptor; cytokines; monocytes; type 2 diabetes mellitus