title: Design and validation of conditional ligands for HLA-B*08:01, HLA-B*15:01, HLA-B*35:01, and HLA-B*44:05.
abstract: We designed conditional ligands restricted to HLA-B*08:01, -B*35:01, and -B*44:05 and proved the use of a conditional ligand previously designed for HLA-B*15:02 together with HLA-B*15:01. Furthermore, we compared the detection capabilities of specific HLA-B*15:01-restricted T cells using the HLA-B*15:01 and HLA-B*15:02 major histocompatibility complex (MHC) multimers and found remarkable differences in the staining patterns detected by flow cytometry. These new conditional ligands greatly add to the application of MHC-based technologies in the analyses of T-cell recognition as they represent frequently expressed HLA-B molecules. This expansion of conditional ligands is important to allow T-cell detection over a wide range of HLA restrictions, and provide comprehensive understanding of the T-cell recognition in a given context.
authors.affiliation: Department of Hematology, Center for Cancer Immune Therapy, Herlev University Hospital, Copenhagen, Denmark.|Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.|Section for Immunology and Vaccinology, National Veterinary Institute, Technical University of Denmark, Copenhagen, Denmark.
authors.forename: Thomas Mørch
authors.initials: TM
authors.lastname: Frøsig
authors.affiliation: Department of Microbiology, National University of Singapore, Singapore.|Department of Biological Sciences and the Immunology Program, National University of Singapore, Singapore.
authors.forename: Jiawei
authors.initials: J
authors.lastname: Yap
authors.affiliation: Department of Hematology, Center for Cancer Immune Therapy, Herlev University Hospital, Copenhagen, Denmark.
authors.forename: Tina
authors.initials: T
authors.lastname: Seremet
authors.affiliation: Department of Hematology, Center for Cancer Immune Therapy, Herlev University Hospital, Copenhagen, Denmark.|Section for Immunology and Vaccinology, National Veterinary Institute, Technical University of Denmark, Copenhagen, Denmark.
authors.forename: Rikke
authors.initials: R
authors.lastname: Lyngaa
authors.affiliation: Department of Hematology, Center for Cancer Immune Therapy, Herlev University Hospital, Copenhagen, Denmark.|Department of Oncology, Herlev University Hospital, Copenhagen, Denmark.
authors.forename: Inge Marie
authors.initials: IM
authors.lastname: Svane
authors.affiliation: Department of Hematology, Center for Cancer Immune Therapy, Herlev University Hospital, Copenhagen, Denmark.
authors.forename: Per
authors.initials: P
authors.lastname: Thor Straten
authors.affiliation: Department of Hematology, Leiden University Medical Centre, Leiden, The Netherlands.
authors.forename: Mirjam H M
authors.initials: MH
authors.lastname: Heemskerk
authors.affiliation: Section for Immunology and Vaccinology, National Veterinary Institute, Technical University of Denmark, Copenhagen, Denmark.|Department of Microbiology, National University of Singapore, Singapore.
authors.forename: Gijsbert M
authors.initials: GM
authors.lastname: Grotenbreg
authors.affiliation: Department of Hematology, Center for Cancer Immune Therapy, Herlev University Hospital, Copenhagen, Denmark.|Section for Immunology and Vaccinology, National Veterinary Institute, Technical University of Denmark, Copenhagen, Denmark.
authors.forename: Sine Reker
authors.initials: SR
authors.lastname: Hadrup
mesh_terms: D000595:Amino Acid Sequence; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D015797:HLA-B35 Antigen / Q000276:immunology*; D015795:HLA-B8 Antigen / Q000276:immunology*; D006801:Humans; D008024:Ligands; D010455:Peptides / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: C509422:HLA-B*08:01 antigen; C500431:HLA-B*35:01 antigen; D015797:HLA-B35 Antigen; D015795:HLA-B8 Antigen; D008024:Ligands; D010455:Peptides
keywords: CD8 T cells; HLA-B molecules; MHC multimer; conditional ligands; flow cytometry