title: Effect of nonsurgical periodontal treatment in conjunction with either systemic administration of amoxicillin and metronidazole or additional photodynamic therapy on the concentration of matrix metalloproteinases 8 and 9 in gingival crevicular fluid in patients with aggressive periodontitis.
abstract: BACKGROUND
  To evaluate in patients with aggressive periodontitis (AgP) the effect of nonsurgical periodontal treatment in conjunction with either additional administration of systemic antibiotics (AB) or application of photodynamic therapy (PDT) on the gingival crevicular fluid (GCF) concentration of matrix metalloproteinases 8 and 9 (MMP-8 and -9).
  
  
  METHODS
  Thirty-six patients with AgP were included in the study. Patients were randomly assigned to treatment with either scaling and root planing (SRP) followed by systemic administration of AB (e.g. Amoxicillin + Metronidazole) or SRP + PDT. The analysis of MMP-8 and -9 GCF concentrations was performed at baseline and at 3 and 6 months after treatment. Nonparametric U-Mann-Whitney test was used for comparison between groups. Changes from baseline to 3 and 6 months were analyzed with the Friedman's ANOVA test with Kendall's index of consistency.
  
  
  RESULTS
  In the AB group, patients showed a statistically significant (p = 0.01) decrease of MMP-8 GCF level at both 3 and 6 months post treatment. In the PDT group, the change of MMP-8 GCF level was not statistically significant. Both groups showed at 3 and 6 months a decrease in MMP-9 levels. However, this change did not reach statistical significance.
  
  
  CONCLUSIONS
  Within the limits of the present study, it may be suggested that in patients with AgP, nonsurgical periodontal therapy in conjunction with adjunctive systemic administration of amoxicilin and metronidazole is more effective in reducing GCF MMP-8 levels compared to the adjunctive use of PDT.
authors.affiliation: Department of Periodontal and Oral Mucosa Diseases, Medical University of Białystok, ul. Waszyngtona 13, 15-269, Białystok, Poland. annaskurska@wp.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Skurska
authors.affiliation: Department of Periodontal and Oral Mucosa Diseases, Medical University of Białystok, ul. Waszyngtona 13, 15-269, Białystok, Poland. ewadolinska@umb.edu.pl.
authors.forename: Ewa
authors.initials: E
authors.lastname: Dolinska
authors.affiliation: Department of Periodontal and Oral Mucosa Diseases, Medical University of Białystok, ul. Waszyngtona 13, 15-269, Białystok, Poland. mpietruska@wp.pl.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Pietruska
authors.affiliation: Private Practice, ul. Waszyngtona 1/34, 15-269, Białystok, Poland. jpietruski@wp.pl.
authors.forename: Jan K
authors.initials: JK
authors.lastname: Pietruski
authors.affiliation: Department of Clinical Laboratory Diagnostics, Medical University of Białystok, ul. Waszyngtona 15, 15-269, Białystok, Poland. piekarskav@yohoo.com.
authors.forename: Violetta
authors.initials: V
authors.lastname: Dymicka
authors.affiliation: Department of Clinical Laboratory Diagnostics, Medical University of Białystok, ul. Waszyngtona 15, 15-269, Białystok, Poland. kemona@umb.edu.pl.
authors.forename: Halina
authors.initials: H
authors.lastname: Kemona
authors.affiliation: Department of Periodontology, Philipps-University of Marburg, Georg-Voigt-Str. 3, D-35039, Marburg, Germany. arweiler@med.uni-marburg.de.
authors.forename: Nicole B
authors.initials: NB
authors.lastname: Arweiler
authors.affiliation: Department of Statistics and Medical Informatics, Medical University of Białystok, ul. Szpitalna 37, 15-295, Białystok, Poland. robert.milewski@umb.edu.pl.
authors.forename: Robert
authors.initials: R
authors.lastname: Milewsk
authors.affiliation: Department of Periodontology, Dental School University of Bern, Freiburgstrasse 7, 3010, Bern, Switzerland. anton.sculean@zmk.unibe.ch.
authors.forename: Anton
authors.initials: A
authors.lastname: Sculean
mesh_terms: D010520:Aggressive Periodontitis / Q000188:drug therapy / Q000201:enzymology / Q000628:therapy*; D000658:Amoxicillin / Q000008:administration & dosage / Q000627:therapeutic use*; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D000890:Anti-Infective Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D003131:Combined Modality Therapy / Q000379:methods; D012534:Dental Scaling / Q000379:methods; D005260:Female; D005500:Follow-Up Studies; D005883:Gingival Crevicular Fluid / Q000187:drug effects / Q000201:enzymology*; D006801:Humans; D054023:Lasers, Semiconductor / Q000627:therapeutic use; D008297:Male; D020784:Matrix Metalloproteinase 8 / Q000032:analysis / Q000187:drug effects*; D020780:Matrix Metalloproteinase 9 / Q000032:analysis / Q000187:drug effects*; D008795:Metronidazole / Q000008:administration & dosage / Q000627:therapeutic use*; D057747:Periodontal Debridement / Q000379:methods*; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000627:therapeutic use; D011446:Prospective Studies; D016745:Root Planing / Q000379:methods; D016037:Single-Blind Method
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D017319:Photosensitizing Agents; D008795:Metronidazole; D000658:Amoxicillin; C488762:MMP8 protein, human; D020784:Matrix Metalloproteinase 8; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9