title: SDF-1/CXCR4 promotes F5M2 osteosarcoma cell migration by activating the Wnt/β-catenin signaling pathway.
abstract: Osteosarcoma (OS), the most common primary malignant bone tumor in children and adolescents, lacks an effective therapy. Stromal cell-derived factor (SDF-1) and its receptor, CXCR4, play multiple roles in migration, proliferation, and survival of different tumor cells. This study aimed to investigate whether the functional SDF-1/CXCR4 signaling mediates chemotaxis in F5M2 OS cells as well as the underlying mechanisms. Immunohistochemistry and immunofluorescence microscopy were used. RNA expression was detected by real-time quantitative polymerase chain reaction, and protein expression was examined by Western blotting. Migration assays were carried out in F5M2 cells. The results showed that the expression of CXCR4 and β-catenin mRNA and protein was significantly higher in OS tissues compared to the surrounding non-neoplastic tissues. SDF-1 promoted F5M2 cell migration by activating the AKT and Wnt/β-catenin signaling pathway, which was abrogated by preincubation with AMD3100 and LY294002. In conclusion, SDF-1/CXCR4 axis-promoted F5M2 cell migration was regulated by the Wnt/β-catenin signaling pathway.
authors.affiliation: Department of Orthopaedic Surgery, Tangdu Hospital, The Fourth Military Medical University, 569 Xin Si Road, Xi'an, 710038, Shaanxi, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Lu
authors.forename: Bin
authors.initials: B
authors.lastname: Hu
authors.forename: Guo-Feng
authors.initials: GF
authors.lastname: Guan
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Chun-qiu
authors.initials: CQ
authors.lastname: Wang
authors.forename: Qiong
authors.initials: Q
authors.lastname: Ma
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Wen
authors.forename: Xiu-Chun
authors.initials: XC
authors.lastname: Qiu
authors.forename: Xiao-ping
authors.initials: XP
authors.lastname: Zhang
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D000293:Adolescent; D000328:Adult; D001859:Bone Neoplasms / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D054377:Chemokine CXCL12 / Q000235:genetics*; D002633:Chemotaxis / Q000235:genetics; D002648:Child; D005260:Female; D006801:Humans; D008297:Male; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics; D019718:Receptors, CXCR4 / Q000235:genetics*; D060449:Wnt Signaling Pathway / Q000235:genetics*; D055815:Young Adult; D051176:beta Catenin / Q000235:genetics*
chemical_list: C495270:CTNNB1 protein, human; C515255:CXCL12 protein, human; C527276:CXCR4 protein, human; D054377:Chemokine CXCL12; D012333:RNA, Messenger; D019718:Receptors, CXCR4; D051176:beta Catenin