title: Establishment and characterization of a novel murine model for pollen allergy.
abstract: Although there have been many studies revealing the mechanism and establishing the therapeutical method for allergic rhinitis, no suitable animal models for allergic rhinitis, especially for pollen allergy, are currently available. We therefore aimed in this study to develop a murine model producing IgE in response to an inhaled antigen without using any adjuvants. Ovalbumin (OVA)-specific T cell receptor transgenic mice (DO11.10) inhaled an OVA solution for one h, twice a week, for six weeks. The resulting increase of OVA-specific IgE in the serum was observed depending on the times of inhalation. Spleen cells from mice that had inhaled the antigen produced more IL-4 and less IFN-γ than those from the control mice in vitro. These results indicate that inhaled antigen enhanced the Th2-type responses and induced IgE production in a T cell-mediated manner. Our findings would contribute to studies on prevention and treatment of pollen allergy.
authors.affiliation: a Department of Applied Biological Science , Tokyo University of Agriculture and Technology , Tokyo , Japan.
authors.forename: Shiho
authors.initials: S
authors.lastname: Murakami
authors.forename: Sayuri
authors.initials: S
authors.lastname: Nakayama
authors.forename: Makoto
authors.initials: M
authors.lastname: Hattori
authors.forename: Tadashi
authors.initials: T
authors.lastname: Yoshida
mesh_terms: D000485:Allergens / Q000276:immunology; D000818:Animals; D000941:Antigens / Q000276:immunology; D004195:Disease Models, Animal; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood* / Q000276:immunology; D007371:Interferon-gamma / Q000096:biosynthesis / Q000276:immunology; D015847:Interleukin-4 / Q000096:biosynthesis / Q000276:immunology; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D010047:Ovalbumin / Q000276:immunology*; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D065631:Rhinitis, Allergic / Q000276:immunology* / Q000473:pathology / Q000628:therapy; D006255:Rhinitis, Allergic, Seasonal / Q000235:genetics / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology
chemical_list: D000485:Allergens; D000941:Antigens; D011948:Receptors, Antigen, T-Cell; D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma; D010047:Ovalbumin
keywords: IgE; TCR transgenic mice; Th2 response; inhaled antigen; pollen allergy