title: Antioxidant activity of whey protein hydrolysates in milk beverage system.
abstract: The aim of the present study was to evaluate the antioxidant activity of flavoured milk enriched with antioxidative whey protein hydrolysates (WPHs) by radical scavenging method. Whey protein concentrate (WPC) was hydrolyzed by using three commercial proteases; flavouzyme, alcalase and corolase PP and these WPHs were analyzed for degree of hydrolysis and antioxidant activity. The antioxidant activities of these WPHs were evaluated using ABTS method. Trolox equivalent antioxidant activity of all the hydrolysates i.e. flavourzyme (0.81 ± 0.04), alcalase (1.16 ± 0.05) and corolase (1.42 ± 0.12) was higher than the WPC (0.19 ± 0.01). Among these, whey protein hydrolysates prepared using corolase showed maximum antioxidant activity. Total 15 β-lactoglobulin, 1 α-lactoalbumin, and 6 β-casein derived peptide fragments were identified in the WPHs by LC-MS/MS. Due to their size and characteristic amino acid composition, all the identified peptides may contribute for the antioxidant activity. The strawberry and chocolate flavoured milk was supplemented with WPC and WPHs and 2 % addition has shown increase in antioxidant activity upto 42 %. The result suggests that WPH could be used as natural biofunctional ingredients in enhancing antioxidant properties of food products.
authors.affiliation: Dairy Chemistry Department, National Dairy Research Institute, Karnal, 132001 Haryana India.
authors.forename: Bimlesh
authors.initials: B
authors.lastname: Mann
authors.affiliation: Dairy Chemistry Department, National Dairy Research Institute, Karnal, 132001 Haryana India.
authors.forename: Anuradha
authors.initials: A
authors.lastname: Kumari
authors.affiliation: Dairy Chemistry Department, National Dairy Research Institute, Karnal, 132001 Haryana India.
authors.forename: Rajesh
authors.initials: R
authors.lastname: Kumar
authors.affiliation: Dairy Chemistry Department, National Dairy Research Institute, Karnal, 132001 Haryana India.
authors.forename: Rajan
authors.initials: R
authors.lastname: Sharma
authors.affiliation: Dairy Chemistry Department, National Dairy Research Institute, Karnal, 132001 Haryana India.
authors.forename: Kishore
authors.initials: K
authors.lastname: Prajapati
authors.affiliation: Dairy Chemistry Department, National Dairy Research Institute, Karnal, 132001 Haryana India.
authors.forename: Shaik
authors.initials: S
authors.lastname: Mahboob
authors.affiliation: Dairy Chemistry Department, National Dairy Research Institute, Karnal, 132001 Haryana India.
authors.forename: S
authors.initials: S
authors.lastname: Athira
keywords: Alcalase; Antioxidant activity; Corolase; Flavourzyme; Whey protein hydrolysates