title: Identification of Development and Pathogenicity Related Gene in Botrytis cinerea via Digital Gene Expression Profile.
abstract: BACKGROUND
  Botrytis cinerea, a haploid Euascomycete fungus that infects numerous crops, has been used as a model system for studying molecular phytopathology. Botrytis cinerea adopts various modes of infection, which are mediated by a number of pathogenicity and virulence-related genes. Many of these genes have not been reported previously.
  
  
  OBJECTIVE
  This study aimed to investigate development and pathogenicity-related genes between a novel nonpathogenic mutant and the Wild Type (WT) in B. cinerea.
  
  
  METHODS
  Digital Gene Expression (DGE) tag profiling can reveal novel genes that may be involved in development and pathogenicity of plant pathogen. A large volume of B. cinerea tag-seq was generated to identify differential expressed genes by the Illumina DGE tag proﬁling technology.
  
  
  RESULTS
  A total of 4,182,944 and 4,182,021 clean tags were obtained from the WT and a nonpathogenic mutant stain (BCt89), respectively, and 10,410 differentially expressed genes were identified. In addition, 84 genes were expressed in the WT only while 34 genes were expressed in the mutant only. A total of 664 differentially expressed genes were involved in 91 Kyoto Encyclopedia of Genes and Genome pathways, including signaling and metabolic pathways.
  
  
  CONCLUSIONS
  Expression levels of 1,426 genes were significantly up-regulated in the mutant compared to WT. Furthermore, 301 genes were down-regulated with False Discovery Rates (FDR) of < 0.001 and absolute value of log2 Ratio of ≥ 1.
authors.affiliation: Mycotoxin and Molecular Plant Pathology Laboratory, Agricultural University of Hebei, Baoding, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Mycotoxin and Molecular Plant Pathology Laboratory, Agricultural University of Hebei, Baoding, China.
authors.forename: He Long
authors.initials: HL
authors.lastname: Si
authors.affiliation: Mycotoxin and Molecular Plant Pathology Laboratory, Agricultural University of Hebei, Baoding, China.
authors.forename: Zhi Ying
authors.initials: ZY
authors.lastname: Sun
authors.affiliation: Mycotoxin and Molecular Plant Pathology Laboratory, Agricultural University of Hebei, Baoding, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Institute of Pomology, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang, China.
authors.forename: Zhan
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Mycotoxin and Molecular Plant Pathology Laboratory, Agricultural University of Hebei, Baoding, China.
authors.forename: Jin Lin
authors.initials: JL
authors.lastname: Zhang
authors.affiliation: Mycotoxin and Molecular Plant Pathology Laboratory, Agricultural University of Hebei, Baoding, China.
authors.forename: Ji Hong
authors.initials: JH
authors.lastname: Xing
authors.affiliation: Mycotoxin and Molecular Plant Pathology Laboratory, Agricultural University of Hebei, Baoding, China.
authors.forename: Jin Gao
authors.initials: JG
authors.lastname: Dong
keywords: Botrytis cinerea; Development and Growth; Digital Gene Expression Profile; Pathogenicity