title: Lipoteichoic acid isolated from Lactobacillus plantarum inhibits melanogenesis in B16F10 mouse melanoma cells.
abstract: Lipoteichoic acid (LTA) is a major component of the cell wall of Gram-positive bacteria. Its effects on living organisms are different from those of lipopolysaccharide (LPS) found in Gram-negative bacteria. LTA contributes to immune regulatory effects including anti-aging. In this study, we showed that LTA isolated from Lactobacillus plantarum (pLTA) inhibited melanogenesis in B16F10 mouse melanoma cells. pLTA reduced the cellular activity of tyrosinase and the expression of tyrosinase family members in a dose-dependent manner. The expression of microphthalmia-associated transcription factor (MITF), a key factor in the synthesis of melanin, was also decreased by pLTA. Further, we showed that pLTA activated melanogenesis signaling, such as extracellular signal-regulated kinase (ERK) and phosphatidylinositol 3-kinse (PI3K)/AKT. In addition, the expression of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) and HuR, which are important RNA-binding proteins (RBPs), was reduced. pLTA likely degrades MITF via regulation of melanogenic signaling and RNA stability of melanogenic proteins, resulting in the reduction of melanin. Thus, our data suggest that pLTA has therapeutic potential for treating hyperpigmentation disorders and can also be used as a cosmetic whitening agent.
authors.affiliation: School of Biotechnology and Institute of Life Science and Resources, Yongin 449-701, Korea.
authors.forename: Hye Rim
authors.initials: HR
authors.lastname: Kim
authors.affiliation: School of Biotechnology and Institute of Life Science and Resources, Yongin 449-701, Korea.|RNA Inc., College of Life Science, Kyung Hee University, Yongin 449-701, Korea.
authors.forename: Hangeun
authors.initials: H
authors.lastname: Kim
authors.affiliation: School of Biotechnology and Institute of Life Science and Resources, Yongin 449-701, Korea.
authors.forename: Bong Jun
authors.initials: BJ
authors.lastname: Jung
authors.affiliation: School of Biotechnology and Institute of Life Science and Resources, Yongin 449-701, Korea.
authors.forename: Ga Eun
authors.initials: GE
authors.lastname: You
authors.affiliation: Institute Pasteur Korea, Seongnam 463-400, Korea.
authors.forename: Soojin
authors.initials: S
authors.lastname: Jang
authors.affiliation: School of Biotechnology and Institute of Life Science and Resources, Yongin 449-701, Korea ; RNA Inc., College of Life Science, Kyung Hee University, Yongin 449-701, Korea ; Skin Biotechnology Center, Gyeonggi Biocenter, Suwon 443-766, Korea.
authors.forename: Dae Kyun
authors.initials: DK
authors.lastname: Chung
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002477:Cells; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D048191:Lactiplantibacillus plantarum / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D008545:Melanoma / Q000201:enzymology* / Q000473:pathology; D051379:Mice; D051739:Microphthalmia-Associated Transcription Factor / Q000235:genetics / Q000378:metabolism; D013682:Teichoic Acids / Q000494:pharmacology*
chemical_list: D008070:Lipopolysaccharides; D051739:Microphthalmia-Associated Transcription Factor; C497235:Mitf protein, mouse; D013682:Teichoic Acids; C009900:lipoteichoic acid
keywords: Lactobacillus plantarum; RNA stability; lipoteichoic acid; melanogenesis; microphthalmia-associated transcription factor (MITF)