title: Genotype screening of APLN rs3115757 variant in Egyptian women population reveals an association with obesity and insulin resistance.
abstract: OBJECTIVE
  Apelin is one of adipokines that plays a pivotal role in energy metabolism, insulin sensitivity and vascular integrity. A definite genetic variant of apelin gene (APLN), rs3115757, was recently introduced to potentially influence apelin expression in adipocytes. The aim of our study was to explore the sights of a potential association of this functional variant with obesity traits, insulin resistance indices as well as type 2 diabetes mellitus (T2DM) prevalence.
  
  
  METHODS
  Genotype screening for rs3115757 variant in 151 Egyptian female samples was conducted. Fasting levels of serum insulin and lipid profile, in addition to plasma glucose were measured. Cochran-Armitage trend test was used to decide the risk allele and evaluate the association between the candidate variant and obesity using a case-control design.
  
  
  RESULTS
  The homozygous G risk allele carriers showed higher values of body mass index (BMI) and waist circumference (P=0.001,0.02, respectively) as compared to CC or CG genotypes. As for GG genotype carriers, the risk of developing morbid obesity in lean subjects, (BMI<25), is twelve times the risk in subjects carrying other genotypes (OR=12.09, 95% CI: 1.4, 104.8, P=0.024). On the other hand, GG carriers are shown to be more resistant to insulin. Significantly after correction for BMI and age effects, GG genotype carriers showed 14% and 41% increment in insulin level and resistance (OR=1.14, 95% CI: 1.06, 1.23, P=0.001), (OR=1.42, 95% CI: 1.19, 1.70, P<0.001), respectively.
  
  
  CONCLUSIONS
  These results suggest a prospective role mediated by this variant in mounting obesity disorders and as significant as insulin resistance complications.
authors.affiliation: Biochemistry Department, Faculty of Pharmacy, Ain Shams University, Abbassia, Cairo, Egypt.
authors.forename: Mostafa A
authors.initials: MA
authors.lastname: Aboouf
authors.affiliation: Biochemistry Department, Faculty of Pharmacy, Ain Shams University, Abbassia, Cairo, Egypt.
authors.forename: Nadia M
authors.initials: NM
authors.lastname: Hamdy
authors.affiliation: Clinical Pathology and Laboratory Department, National Institute of Diabetes and Endocrinology (NIDE), Cairo, Egypt.
authors.forename: Ashraf I
authors.initials: AI
authors.lastname: Amin
authors.affiliation: Biochemistry Department, Faculty of Pharmacy, Ain Shams University, Abbassia, Cairo, Egypt. Electronic address: hala.elmesallamy@hotmail.com.
authors.forename: Hala O
authors.initials: HO
authors.lastname: El-Mesallamy
mesh_terms: D054392:Adipokines; D000293:Adolescent; D000328:Adult; D000073861:Apelin; D015992:Body Mass Index; D016022:Case-Control Studies; D004252:DNA Mutational Analysis; D003924:Diabetes Mellitus, Type 2 / Q000453:epidemiology / Q000235:genetics; D004534:Egypt / Q000453:epidemiology; D005260:Female; D056726:Genetic Association Studies; D005820:Genetic Testing; D005838:Genotype; D006801:Humans; D007333:Insulin Resistance / Q000235:genetics*; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics*; D008875:Middle Aged; D009765:Obesity / Q000453:epidemiology / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D055105:Waist Circumference / Q000235:genetics; D055815:Young Adult
chemical_list: C115436:APLN protein, human; D054392:Adipokines; D000073861:Apelin; D036341:Intercellular Signaling Peptides and Proteins
keywords: Apelin; Genotyping; Insulin resistance; Metabolic syndrome; Obesity; T2DM