title: Biotransformation of acetophenone and its halogen derivatives byYarrowia lipolyticastrains.
abstract: The ability of 16 strains ofYarrowia lipolyticato biotransform acetophenone and its derivatives has been studied. Thirteen of these strains were derived from a wild-type strainY. lipolyticaA-101; six had the invertase gene (SUC2) fromSaccharomyces cerevisiaeintegrated into their genome, as well as the damaged or undamaged gene encoding orotidine-5'-phosphate decarboxylase (URA3), three had integrated the damagedURA3gene into their genome and three were UV acetate-negative mutants, not able to growth on acetate as the sole carbon source. The other tested strains included two wild strains, A-101 and PMR-1, and an adenine auxotroph ATCC 32-338A. All strains were capable of reducing acetophenone to theR-alcohol in high enantiomeric excess (80-89 %). In all of the cultures tested, reversibility of the reduction was observed, which led to an increase in the enantiomeric excess. nantioselective reduction of the acetophenone halogen derivatives revealed that the nature and location of the halogen atom had a significant influence on the enantioselectivity of the reduction. In the culture of ATCC 32-338A, after a 3-day biotransformation of 2,4'-dibromoacetophenone the enantiopureR-alcohol was obtained at a rate of 100 % of substrate conversion. In conclusion, using these invertase-containing strains or uracyl auxotrophs provided no additional benefit in terms of biotransformation capacity over the parental strain.
authors.affiliation: Department of Chemistry, Wrocław University of Environmental and Life Sciences, Norwida 25, 50-375 Wrocław, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Janeczko
authors.affiliation: Department of Chemistry, Wrocław University of Environmental and Life Sciences, Norwida 25, 50-375 Wrocław, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Bąkowski
authors.affiliation: Department of Biotechnology and Food Microbiology, Wroclaw University of Environmental and Life Sciences, Norwida 25, 50-375 Wrocław, Poland ; Department of Medicine, The Witelon University of Applied Sciences, Sejmowa 5A, 59-220 Legnica, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Walczak
authors.affiliation: Department of Biotechnology and Food Microbiology, Wroclaw University of Environmental and Life Sciences, Norwida 25, 50-375 Wrocław, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Robak
authors.affiliation: Department of Chemistry, Wrocław University of Environmental and Life Sciences, Norwida 25, 50-375 Wrocław, Poland ; Department of Cosmetology, Wrocław College of Physiotherapy, Kościuszki 4, 50-038 Wrocław, Poland.
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Dmochowska-Gładysz
authors.affiliation: Department of Chemistry, Wrocław University of Environmental and Life Sciences, Norwida 25, 50-375 Wrocław, Poland.
authors.forename: Edyta
authors.initials: E
authors.lastname: Kostrzewa-Susłow
keywords: Enantiospecific reduction; Halogen derivatives of acetophenone; SUC2; URA3; Yarrowia lipolytica