title: Preparation and characterization of macroporous monoliths imprinted with erythromycin.
abstract: The synthesis of macroporous molecularly imprinted monoliths was performed using the monomers system 2-hydroxyethyl methacrylate-ethylene glycol dimethacrylate and erythromycin as a template. The copolymerization was carried out in situ inside 50 mm × 4.6 mm i.d. stainless-steel tubing. The morphology of the monoliths was examined with scanning electron microscopy. The porous characteristics were determined both from the data of hydrodynamic permeability of monoliths and by means of mercury intrusion porosimetry. The retention parameters of target substance (erythromycin), values of calculated imprinting factors and apparent dynamic dissociation constants were obtained for monoliths prepared with the application of different amount of template (4, 8 and 12 mol%). The separations of the mixtures azithromycin/erythromycin and ciprofloxacin/erythromycin were demonstrated. Additionally, the possibility of erythromycin quantification in human blood plasma was shown.
authors.affiliation: Institute of Macromolecular Compounds, Russian Academy of Sciences, St. Petersburg, Russia.
authors.forename: E G
authors.initials: EG
authors.lastname: Vlakh
authors.affiliation: Institute of Chemistry, Saint-Petersburg State University, St. Petersburg, Russia.
authors.forename: M A
authors.initials: MA
authors.lastname: Stepanova
authors.affiliation: Institute of Macromolecular Compounds, Russian Academy of Sciences, St. Petersburg, Russia.
authors.forename: O A
authors.initials: OA
authors.lastname: Pisarev
authors.affiliation: Institute of Macromolecular Compounds, Russian Academy of Sciences, St. Petersburg, Russia.
authors.forename: T B
authors.initials: TB
authors.lastname: Tennikova
mesh_terms: D000327:Adsorption; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation; D004917:Erythromycin / Q000097:blood / Q000302:isolation & purification*; D054802:Molecular Imprinting; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D016062:Porosity
chemical_list: D011108:Polymers; D004917:Erythromycin
keywords: Antibiotics; Artificial receptors; High-performance liquid chromatography; Macroporous polymer monoliths; Molecular imprinting