title: [Construction and characterization of an epitope-mutated Asia 1 type foot-and-mouth disease virus].
abstract: To generate an epitope-mutated foot-and-mouth disease virus (FMDV) as a marker vaccine, the infectious clone pAsia 1-FMDV containing the complete genomic cDNA of Asia 1 type FMDV was used as backbone, the residues at positions 27 and 31 in the 3D gene were mutated (H27Y and N31R). The resulting plasmid pAsia 1-FMDV-3DM encoding a mutated epitope was transfected into BHK-21 cells and the recombinant virus rAsia 1-3DM was rescued. The recombinant virus showed similar biological characteristics comparable with the parental virus. In serological neutralization test the antisera against recombine virus have a good reactivity with parental virus. The antisera against the mutant virus were shown to be reactive with the mutated epitope but not the wild-type one. The results indicated that the two virus strains could be distinguished by western blotting using synthetic peptides. This epitope-mutated FMDV strain will be evaluated as a potential marker vaccine against FMDV infections.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yonghao
authors.initials: Y
authors.lastname: Hu
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.forename: Songhao
authors.initials: S
authors.lastname: Wang
authors.forename: Zixiang
authors.initials: Z
authors.lastname: Zhu
authors.forename: Haixue
authors.initials: H
authors.lastname: Zheng
mesh_terms: D000818:Animals; D002460:Cell Line; D018076:DNA, Complementary; D000939:Epitopes / Q000235:genetics; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics*; D007106:Immune Sera; D009500:Neutralization Tests; D014162:Transfection
chemical_list: D018076:DNA, Complementary; D000939:Epitopes; D007106:Immune Sera