title: Effect of Centaurium erythraea Rafn, Artemisia herba-alba Asso and Trigonella foenum-graecum L. on liver fat accumulation in C57BL/6J mice with high-fat diet-induced type 2 diabetes.
abstract: BACKGROUND
  Centaurium erythraea Rafn (CE), Artemisia herba-alba Asso (AHA) and Trigonella foenum-graecum L. (TFG) are traditionally used to treat type 2 diabetes in Algeria, previous studies have found that extracts of these plants were effective to treat or prevent experimental diabetes induced by high-fat diet (HFD).
  
  
  OBJECTIVE
  Describe the additional effects of these extracts on lipid tissue deposition in HFD.
  
  
  METHODS
  Male C57BL/6J mice were fed with HFD to induce type 2 Diabetes. Groups of mice were given plant extracts orally at 2g/kg/bodyweight daily for 20 weeks during establishment of diabetes, or for 18 weeks after confirmation of diabetes at the 17th week. Liver and other tissue samples were stained with Oil Red O.
  
  
  RESULTS
  Liver steatosis was confirmed with HFD. CE, AHA and TFG extracts improved liver steatosis by the end of the preventive (20 weeks) and curative periods (35 weeks). This was most marked for CE extract (p<0.05), less so with TFG and AHA. No steatosis was found in other tissues.
  
  
  CONCLUSIONS
  CE extract had a clear hepatoprotective effect in this mouse model of diet-induced type 2 diabetes. AHA and TFG had a minimal or no significant effect on steatosis. Beyond its effect as an antidiabetic agent, CE may also be promising to prevent or treat non-alcoholic liver steatosis.
authors.affiliation: Département de Pharmacologie, Université de Bordeaux, Bordeaux, France; Département de Nutrition, Université des frères Mentouri, INATAA, Constantine, Algerie. Electronic address: nawelc2001@yahoo.fr.
authors.forename: Nawel
authors.initials: N
authors.lastname: Hamza
authors.affiliation: Département de Pharmacologie, Université de Bordeaux, Bordeaux, France.
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Berke
authors.affiliation: Département de Pharmacologie, Université de Bordeaux, Bordeaux, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Cheze
authors.affiliation: Bordeaux Imaging Center, UMS 3420 CNRS, Université de Bordeaux, Bordeaux, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Marais
authors.affiliation: Département de Pharmacologie, Université de Bordeaux, Bordeaux, France.
authors.forename: Simon
authors.initials: S
authors.lastname: Lorrain
authors.affiliation: Département de Pharmacologie, Université de Bordeaux, Bordeaux, France.
authors.forename: Abdelilah
authors.initials: A
authors.lastname: Abdouelfath
authors.affiliation: Département de Pharmacologie, Université de Bordeaux, Bordeaux, France.
authors.forename: Regis
authors.initials: R
authors.lastname: Lassalle
authors.affiliation: Unité de pathologie fœtoplacentaire, Université de Bordeaux, CHU de Bordeaux, Hôpital Pellegrin, Bordeaux, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Carles
authors.affiliation: Service de Nutrition Diabétologie et Maladies Métaboliques, CHU de Bordeaux, Haut-Levèque, Bordeaux, France.
authors.forename: Henri
authors.initials: H
authors.lastname: Gin
authors.affiliation: Département de Pharmacologie, Université de Bordeaux, Bordeaux, France. Electronic address: nicholas.moore@u-bordeaux.fr.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Moore
mesh_terms: D000818:Animals; D019306:Artemisia; D029966:Centaurium; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D059305:Diet, High-Fat; D005234:Fatty Liver / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D007668:Kidney / Q000033:anatomy & histology / Q000187:drug effects; D050356:Lipid Metabolism / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018482:Muscle, Skeletal / Q000033:anatomy & histology / Q000187:drug effects; D009929:Organ Size / Q000187:drug effects; D008517:Phytotherapy; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D012639:Seeds; D029910:Trigonella
chemical_list: D010936:Plant Extracts
keywords: Artemisia herba-alba; Centaurium erythraea; High fat diet; Mice; Non-alcoholic liver steatosis; Trigonella foenum-graecum; Type 2 diabetes/therapy/prevention