title: Clnk plays a role in TNF-alpha-induced cell death in murine fibrosarcoma cell line L929.
abstract: Clnk, as a third member of the Blnk/SLP-76 adapter family, is involved in the positive regulation of immunoreceptor signaling. Here we provide findings that Clnk may be is required for TNF induced cell death, it functions downstream of RIP3 and promotes TNF- induced ROS generation and MLKL tetramer formation and subsequent necrosis of L929 cells. Therefore, Clnk, as an adaptor protein, may take part in the other cellular processes.
authors.affiliation: State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian 361005, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian 361005, China.
authors.forename: Chengfeng
authors.initials: C
authors.lastname: Cai
authors.affiliation: State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian 361005, China.
authors.forename: Xuli
authors.initials: X
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian 361005, China.
authors.forename: Wanze
authors.initials: W
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian 361005, China. Electronic address: liqinxi@xmu.edu.cn.
authors.forename: Qinxi
authors.initials: Q
authors.lastname: Li
authors.affiliation: State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian 361005, China. Electronic address: huaminzhou@xmu.edu.cn.
authors.forename: Huamin
authors.initials: H
authors.lastname: Zhou
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D002352:Carrier Proteins / Q000276:immunology*; D016923:Cell Death; D045744:Cell Line, Tumor; D005354:Fibrosarcoma / Q000276:immunology* / Q000473:pathology; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D009336:Necrosis / Q000276:immunology; D011494:Protein Kinases / Q000737:chemistry / Q000276:immunology; D055503:Protein Multimerization; D017382:Reactive Oxygen Species / Q000276:immunology*; D053422:Receptor-Interacting Protein Serine-Threonine Kinases / Q000276:immunology; D015398:Signal Transduction; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; C488191:Clnk protein, mouse; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; C570694:MLKL protein, mouse; D011494:Protein Kinases; D053422:Receptor-Interacting Protein Serine-Threonine Kinases; C485615:Ripk3 protein, mouse
keywords: Clnk; MLKL; Necrosis; RIP3; ROS