title: Stability of Asp(B28) Insulin Exposed to Modified and Unmodified Polypropylene.
abstract: Polypropylene (PP) plates have been modified with two different hydrophilic polymeric materials, poly(N,N-dimethylacrylamide) (poly(DMAAm)) and poly(poly(ethylene glycol)methacrylate) (poly(PEGMA)) in order to reduce insulin adsorption when the plates were exposed to insulin aspart (Asp(B28) insulin). The influence of surface modification on the chemical and physical stability of Asp(B28) insulin was evaluated by two chromatographic methods, size exclusion chromatography (SEC) and reverse phase high pressure liquid chromatography (RP-HPLC) and the Thioflavin T assay. A clear difference in the stability of Asp(B28) insulin was observed between the three tested surfaces. PP coated with poly(DMAAm) resulted in a poor chemical stability and a significantly improved physical stability compared with unmodified PP. In addition to this a lower phenol concentration was observed for the poly(DMAAm) coating. The results from the poly(PEGMA) coating looked very promising with respect to the stability of Asp(B28) insulin in comparison with the data from unmodified PP and the poly(DMAAm) coating. Two hydrophilic coatings have been tested and surprisingly a difference in Asp(B28) insulin stability was observed. Therefore, Asp(B28) insulin adsorption and stability will be influenced by more than the hydrophilicity of the surface.
authors.forename: Charlotte J
authors.initials: CJ
authors.lastname: Fristrup
authors.forename: Katja
authors.initials: K
authors.lastname: Jankova
authors.forename: Rüya
authors.initials: R
authors.lastname: Eskimergen
authors.forename: Jens T
authors.initials: JT
authors.lastname: Bukrinsky
authors.affiliation: Danish Polymer Centre, Department of Chemical and Biochemical Engineering, Building 227, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark. sh@kt.dtu.dk.
authors.forename: Soren
authors.initials: S
authors.lastname: Hvilsted
mesh_terms: D000178:Acrylamides / Q000737:chemistry; D019943:Amino Acid Substitution; D001224:Aspartic Acid; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007328:Insulin / Q000737:chemistry* / Q000235:genetics*; D008689:Methacrylates / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011126:Polypropylenes / Q000737:chemistry* / Q000494:pharmacology*; D055550:Protein Stability / Q000187:drug effects
chemical_list: D000178:Acrylamides; D007328:Insulin; D008689:Methacrylates; D011126:Polypropylenes; C429790:poly(N,N-dimethylacrylamide); C524499:polyethylene glycol methacrylate; D001224:Aspartic Acid; D011092:Polyethylene Glycols