title: Differentiation of transforming growth factor β1-induced mesenchymal stem cells into nucleus pulposus-like cells under simulated microgravity conditions.
abstract: This study aims to observe the differentiation effects of transforming growth factor β1 (TGF-β1)-transfected bone marrow mesenchymal stem cells (BMMSCs) into the nucleus pulposus-like cells under simulated microgravity conditions. BMMSCs were isolated from the adult rabbits, then cultured and proliferated to the third generation. After transfected by TGF-β1, these MMSCs were incubated in the calcium alginate gel microspheres and rotary cell culture system. The content changes of TGF-β1 inside BMMSCs, as well as the proliferation abilities of BMMSCs, were detected. The immunohistochemistry and toluidine blue staining were performed to detect the type II collagen. The RT-PCR method was performed to detect the expressions of proteoglycan and type II collagen mRNA. Results found that, on the 14th day, the polygonal nucleus pulposus-like cells could be observed. The TGF-β1 content inside the supernatant and the DNA content inside BMMSCs were significantly increased (P<0.05). The immunohistochemistry staining exhibited the positive results about the type II collagen. RT-PCR showed the expressions of proteoglycan and type II collagen mRNA inside BMMSCs. The TGF-β1-transfected BMSCs exhibited the increased synthesizing abilities of proteoglycan and type II collagen under simulated microgravity conditions than the control group.
authors.affiliation: The First Affiliated Hospital of Harbin Medical University Department of Orthopedics Harbin China chenglonghancn&commat;163.com.
authors.forename: C
authors.initials: C
authors.lastname: Han
authors.affiliation: The First Affiliated Hospital of Harbin Medical University Department of Orthopedics Harbin China.
authors.forename: C
authors.initials: C
authors.lastname: Jiang
authors.affiliation: The First Affiliated Hospital of Harbin Medical University Department of Orthopedics Harbin China.
authors.forename: C
authors.initials: C
authors.lastname: Yu
authors.affiliation: The First Affiliated Hospital of Harbin Medical University Department of Orthopedics Harbin China.
authors.forename: H
authors.initials: H
authors.lastname: Shen
mesh_terms: D000464:Alginates; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D024043:Collagen Type II / Q000235:genetics / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D007403:Intervertebral Disc / Q000166:cytology / Q000378:metabolism*; D055959:Intervertebral Disc Degeneration / Q000473:pathology; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008863:Microspheres; D011509:Proteoglycans / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods; D014162:Transfection; D053773:Transforming Growth Factor beta1 / Q000235:genetics* / Q000378:metabolism; D018474:Weightlessness Simulation
chemical_list: D000464:Alginates; D024043:Collagen Type II; D016207:Cytokines; D006603:Hexuronic Acids; D011509:Proteoglycans; D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1; D020723:Glucuronic Acid