title: Phage display for identifying peptides that bind the spike protein of transmissible gastroenteritis virus and possess diagnostic potential.
abstract: The spike (S) protein of porcine transmissible gastroenteritis virus (TGEV) is located within the viral envelope and is the only structural protein that possesses epitopes capable of inducing virus-neutralizing antibodies. Among the four N-terminal antigenic sites A, B, C, and D, site A and to a lesser extent site D (S-AD) induce key neutralizing antibodies. Recently, we expressed S-AD (rS-AD) in recombinant form. In the current study, we used the rS-AD as an immobilized target to identify peptides from a phage-display library with application for diagnosis. Among the 9 phages selected that specifically bound to rS-AD, the phage bearing the peptide TLNMHLFPFHTG bound with the highest affinity and was subsequently used to develop a phage-based ELISA for TGEV. When compared with conventional antibody-based ELISA, phage-mediated ELISA was more sensitive; however, it did not perform better than semi-quantitative RT-PCR, though phage-mediated ELISA was quicker and easier to set up.
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, Harbin, 150030, China, gaowa1128@sina.com.
authors.forename: Siqingaowa
authors.initials: S
authors.lastname: Suo
authors.forename: Xue
authors.initials: X
authors.lastname: Wang
authors.forename: Dante
authors.initials: D
authors.lastname: Zarlenga
authors.forename: Ri-E
authors.initials: RE
authors.lastname: Bu
authors.forename: Yudong
authors.initials: Y
authors.lastname: Ren
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Ren
mesh_terms: D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005761:Gastroenteritis, Transmissible, of Swine / Q000175:diagnosis* / Q000821:virology; D019151:Peptide Library; D010455:Peptides / Q000302:isolation & purification* / Q000378:metabolism*; D011485:Protein Binding; D012680:Sensitivity and Specificity; D064370:Spike Glycoprotein, Coronavirus / Q000378:metabolism*; D013552:Swine; D005760:Transmissible gastroenteritis virus / Q000302:isolation & purification*
chemical_list: D019151:Peptide Library; D010455:Peptides; D064370:Spike Glycoprotein, Coronavirus