title: Notch Transmembrane Domain: Secondary Structure and Topology.
abstract: The Notch signaling pathway is critical in development, neuronal maintenance, and hematopoiesis. An obligate step in the activation of this pathway is cleavage of its transmembrane (TM) domain by γ-secretase. While the soluble domains have been extensively studied, little has been done to characterize its TM and flanking juxtamembrane (JM) segments. Here, we present the results of nuclear magnetic resonance (NMR) studies of the human Notch1 TM/JM domain. The TM domain is largely α-helical. While the flanking JM segments do not adopt regular secondary structure, they interact with the membrane surface, suggesting membrane interactions may play a role in modulating its cleavage by γ-secretase and subsequent NOTCH signaling function.
authors.affiliation: Department of Biochemistry, Center for Structural Biology, and Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-8725, United States.
authors.forename: Catherine L
authors.initials: CL
authors.lastname: Deatherage
authors.affiliation: Department of Biochemistry, Center for Structural Biology, and Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-8725, United States.
authors.forename: Zhenwei
authors.initials: Z
authors.lastname: Lu
authors.affiliation: Department of Biochemistry, Center for Structural Biology, and Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-8725, United States.
authors.forename: Ji-Hun
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Biochemistry, Center for Structural Biology, and Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-8725, United States.
authors.forename: Charles R
authors.initials: CR
authors.lastname: Sanders
mesh_terms: D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D008244:Lysophosphatidylcholines / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010713:Phosphatidylcholines / Q000737:chemistry / Q000378:metabolism; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D051881:Receptor, Notch1 / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013499:Surface Properties
chemical_list: D008051:Lipid Bilayers; D008244:Lysophosphatidylcholines; C497761:NOTCH1 protein, human; D010446:Peptide Fragments; D010713:Phosphatidylcholines; D051881:Receptor, Notch1; D011994:Recombinant Proteins