title: Ammonium fluoride as a mobile phase additive in aqueous normal phase chromatography.
abstract: The use of ammonium fluoride as a mobile phase additive in aqueous normal phase chromatography with silica hydride-based stationary phases and mass spectrometry detection is evaluated. Retention times, peak shape, efficiency and peak intensity are compared to the more standard additives formic acid and ammonium formate. The test solutes were NAD, 3-hydroxyglutaric acid, α-ketoglutaric acid, p-aminohippuric acid, AMP, ATP, aconitic acid, threonine, N-acetyl carnitine, and 3-methyladipic acid. The column parameters are assessed in both the positive and negative ion detection modes. Ammonium fluoride is potentially an aggressive mobile phase additive that could have detrimental effects on column lifetime. Column reproducibility is measured and the effects of switching between different additives are also tested.
authors.affiliation: Department of Chemistry, San Jose State University, San Jose, CA 95192, USA. Electronic address: joseph.pesek@sjsu.edu.
authors.forename: Joseph J
authors.initials: JJ
authors.lastname: Pesek
authors.affiliation: Department of Chemistry, San Jose State University, San Jose, CA 95192, USA.
authors.forename: Maria T
authors.initials: MT
authors.lastname: Matyska
mesh_terms: D064751:Ammonium Compounds; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002845:Chromatography; D005459:Fluorides / Q000737:chemistry*; D013058:Mass Spectrometry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D015203:Reproducibility of Results; D017640:Silicates / Q000737:chemistry; D012996:Solutions / Q000032:analysis; D014867:Water / Q000737:chemistry*
chemical_list: D064751:Ammonium Compounds; D000644:Quaternary Ammonium Compounds; D017640:Silicates; D012996:Solutions; C487012:silica hydride; D014867:Water; C024822:ammonium fluoride; D005459:Fluorides
keywords: Mass spectrometry; Mobile phase additive; Silica hydride