title: Next-Generation Sequencing for Antenatal Prediction of KEL1 Blood Group Status.
abstract: The KEL1 antigen can give rise to immunization of KEL2 mothers. Maternal antibodies can be transferred to the fetus and destroy fetal red blood cells and their stem cell precursors and give rise to serious fetal disease. It is important to be able to predict the fetal KEL status in order to intervene in those pregnancies where the fetus is at risk, and to ascertain when the fetus is not at risk. Technically it can be demanding to predict KEL1 status from a maternal blood sample. The KEL1 allele is based on a single SNP present in about 1-10 % of cell-free maternal DNA after gestation week 10. Here we describe our protocol for antenatal prediction of fetal KEL1 status by NGS analysis of maternal DNA on a MiSeq instrument.
authors.affiliation: Department of Clinical Immunology, Section 2034, Rigshospitalet, Copenhagen University Hospital, Blegdamsvej 9, Copenhagen, 2100, Denmark, kr@rh.dk.
authors.forename: Klaus
authors.initials: K
authors.lastname: Rieneck
authors.forename: Frederik Banch
authors.initials: FB
authors.lastname: Clausen
authors.forename: Morten Hanefeld
authors.initials: MH
authors.lastname: Dziegiel
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers / Q000235:genetics; D005260:Female; D005315:Fetal Diseases / Q000097:blood / Q000235:genetics*; D005333:Fetus / Q000378:metabolism; D005838:Genotype; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D007626:Kell Blood-Group System / Q000097:blood / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*
chemical_list: D017931:DNA Primers; D007626:Kell Blood-Group System; D004247:DNA