title: Double-helix micro-channels on microfluidic chips for enhanced continuous on-chip derivatization followed by electrophoretic separation.
abstract: Micro-channels that contain a special inner structure are critical for efficient mixing and chemical reactions. In this paper, we described the facile fabrication of an integrated microchip with double-helix type micro-channels to improve mixing efficiency and to facilitate multi-step derivatization reactions prior to electrophoretic separation. With a prepared microchip, reagents, samples and reaction products could be driven through micro-channels by siphon, and no other pumping device was necessary. To test its performance, reductive amination of aldehydes with 8-aminonaphthalene-1,3,6-trisulfonate acid disodium (ANTS) was attempted via microchip electrophoresis with laser induced fluorescence (LIF). The effect of the geometry of the reaction micro-channel on the reaction's efficiency was evaluated. Under the selected conditions, successful derivatization of five aldehydes was realized for highly reproducible analysis. The relative standard deviations of the peak areas for 30 consecutive injections were in the range of 0.28-1.61%. The method was applied for the determination of aldehydes in real samples with standard addition recoveries of 87.8-102.8%. Good tolerance of organic solvents was achieved, and the proposed method can potentially be employed for rapid screening of excessively added aldehyde food flavoring.
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, Department of Chemistry, Lanzhou University, Lanzhou, Gansu 730000, China.
authors.forename: Xianglu
authors.initials: X
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, Department of Chemistry, Lanzhou University, Lanzhou, Gansu 730000, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, Department of Chemistry, Lanzhou University, Lanzhou, Gansu 730000, China.
authors.forename: Jinxiu
authors.initials: J
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, Department of Chemistry, Lanzhou University, Lanzhou, Gansu 730000, China.
authors.forename: Shenghong
authors.initials: S
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, Department of Chemistry, Lanzhou University, Lanzhou, Gansu 730000, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Ding
authors.affiliation: Beijing Key Laboratory for Green Catalysis and Separation, Department of Chemistry and Chemical Engineering, Beijing University of Technology, Beijing 100124, China.
authors.forename: Xiayan
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, Department of Chemistry, Lanzhou University, Lanzhou, Gansu 730000, China. Electronic address: puqs@lzu.edu.cn.
authors.forename: Qiaosheng
authors.initials: Q
authors.lastname: Pu
mesh_terms: D000447:Aldehydes / Q000032:analysis* / Q000302:isolation & purification; D000586:Amination; D002099:Cacao / Q000737:chemistry; D002523:Edible Grain / Q000737:chemistry; D046209:Electrophoresis, Microchip / Q000295:instrumentation*; D004867:Equipment Design; D005421:Flavoring Agents / Q000032:analysis* / Q000302:isolation & purification; D005453:Fluorescence; D005504:Food Analysis / Q000295:instrumentation*; D007834:Lasers; D009281:Naphthalenes / Q000737:chemistry*; D010084:Oxidation-Reduction
chemical_list: D000447:Aldehydes; D005421:Flavoring Agents; D009281:Naphthalenes; C046465:8-amino-1,3,6-naphthalenetrisulfonic acid
keywords: Aldehyde flavors; Integrated microchips; Laser induced fluorescence; Microchip electrophoresis; On-chip multistep derivatization