title: Intra-nucleus accumbens administration of the calcium/calmodulin-dependent protein kinase II inhibitor AIP induced antinociception in rats with mononeuropathy.
abstract: Calcium/calmodulin-dependent protein kinase II (CaMKII) is a serine/threonine- dependent protein kinase, which has been implicated in pain modulation at different levels of the central nervous system. The present study was performed in rats with mononeuropathy induced by left common sciatic nerve ligation. Unilateral sciatic nerve loose ligation produced decreases in the hindpaw withdrawal latency (HWL) to noxious thermal and mechanical stimulation. Intra-nucleus accumbens (NAc) injection of 3 μg, 6 μg and 12 μg of myristoylated autocamtide-2-inhibitory peptide (AIP), the CaMKII inhibitor, dose-dependently increased the HWL to noxious thermal and mechanical stimulation in rats with mononeuropathy. Furthermore, intra-NAc administration of morphine, the HWL to noxious thermal and mechanical stimulation increased markedly, and there were no significant differences between morphine group and AIP group. Taken together, the results showed that intra-NAc injection of AIP induced significant antinociceptive effects in rats with mononeuropathy, indicating that CaMKII may play an important role in the transmission and/or modulation of nociceptive information in the NAc in rats with mononeuropathy.
authors.affiliation: Neurobiology Laboratory and State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing 100871, PR China; Kunming Medical University, Kunming 650500, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Bian
authors.affiliation: Neurobiology Laboratory and State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing 100871, PR China. Electronic address: yulc@pku.edu.cn.
authors.forename: Long-Chuan
authors.initials: LC
authors.lastname: Yu
mesh_terms: D000700:Analgesics / Q000627:therapeutic use*; D000818:Animals; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000378:metabolism*; D006358:Hot Temperature; D008297:Male; D020422:Mononeuropathies / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D009020:Morphine / Q000627:therapeutic use; D009714:Nucleus Accumbens / Q000187:drug effects* / Q000378:metabolism; D010455:Peptides / Q000627:therapeutic use*; D010812:Physical Stimulation; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000293:injuries; D014110:Touch
chemical_list: D000700:Analgesics; C503464:CaMKII inhibitor AIP; D010455:Peptides; D009020:Morphine; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2
keywords: Antinociceptive effect; Calcium/calmodulin-dependent protein kinase II (CaMKII); Hindpaw withdrawal latency; Mononeuropathy; Myristoylated autocamtide-2-inhibitory peptide (AIP); Nucleus accumbens