title: Pilot study on novel skin care method by augmentation with Staphylococcus epidermidis, an autologous skin microbe--A blinded randomized clinical trial.
abstract: OBJECTIVE
  Staphylococcus epidermidis is an autologous bacterium that is beneficial to skin health. Our goal was to develop a novel, personalized basic cosmetic that exploits this characteristic.
  
  
  METHODS
  We conducted a double-blinded, randomized clinical trial on augmentation with S. epidermidis as a pilot study, in which S. epidermidis was collected from the subject, cultured for proliferation, and then continuously applied to the subject's own face before sleep twice per week for four weeks in order to increase colonization levels.
  
  
  RESULTS
  The results showed that this treatment increased the lipid content of the skin and suppressed water evaporation, thereby markedly improving skin moisture retention. Moreover, augmentation with S. epidermidis maintained a low acidic condition on the skin surface. The low risk of undesirable effects induced by augmentation with S. epidermidis was also confirmed by measuring erythema and melanin levels.
  
  
  CONCLUSIONS
  These results may serve as a driving force to accelerate the development of novel, personalized basic cosmetics.
authors.affiliation: Department of Biochemistry, Faculty of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki 859-3298, Japan. Electronic address: nodake@niu.ac.jp.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Nodake
authors.affiliation: Department of Biochemistry, Faculty of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki 859-3298, Japan; Research and Development Department, BIOGENOMICS Co., Ltd., 147-40 Masuragahara, Omura, Nagasaki 856-0022, Japan.
authors.forename: Saki
authors.initials: S
authors.lastname: Matsumoto
authors.affiliation: Department of Biochemistry, Faculty of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki 859-3298, Japan; Research and Development Department, BIOGENOMICS Co., Ltd., 147-40 Masuragahara, Omura, Nagasaki 856-0022, Japan.
authors.forename: Ryoko
authors.initials: R
authors.lastname: Miura
authors.affiliation: Research and Development Department, BIOGENOMICS Co., Ltd., 147-40 Masuragahara, Omura, Nagasaki 856-0022, Japan.
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Honda
authors.affiliation: Department of Nutrition, Kyushu Women's University, 1-1 Jiyugaoka, Yahatanishi-ku, Kitakyushu 807-8586, Japan.
authors.forename: Genji
authors.initials: G
authors.lastname: Ishibashi
authors.affiliation: Applied Technology Division, Industrial Technology Center of Nagasaki, 2-1303-8 Ikeda, Omura, Nagasaki 856-0026, Japan.
authors.forename: Shuzo
authors.initials: S
authors.lastname: Matsumoto
authors.affiliation: Department of Dermatology, Medical Centre East, Tokyo Women's Medical University, 2-1-10 Nishi-Ogu, Arakawa, Tokyo 116-8567, Japan.
authors.forename: Itaru
authors.initials: I
authors.lastname: Dekio
authors.affiliation: Department of Biochemistry, Faculty of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki 859-3298, Japan.
authors.forename: Ryuzo
authors.initials: R
authors.lastname: Sakakibara
mesh_terms: D000287:Administration, Topical; D000328:Adult; D003358:Cosmetics / Q000008:administration & dosage; D004311:Double-Blind Method; D004890:Erythema / Q000382:microbiology; D005260:Female; D006801:Humans; D008543:Melanins / Q000502:physiology; D008875:Middle Aged; D010865:Pilot Projects; D012867:Skin / Q000382:microbiology*; D017592:Skin Care / Q000379:methods*; D013212:Staphylococcus epidermidis / Q000302:isolation & purification / Q000502:physiology*; D055815:Young Adult
chemical_list: D003358:Cosmetics; D008543:Melanins
keywords: Augmentation with S. epidermidis; Basic cosmetics; Skin care; Skin microbiota; Skin moisture retention; Staphylococcus epidermidis