title: Conversion of crude Jatropha curcas seed oil into biodiesel using liquid recombinant Candida rugosa lipase isozymes.
abstract: The versatile Candida rugosa lipase (CRL) has been widely used in biotechnological applications. However, there have not been feasibility reports on the transesterification of non-edible oils to produce biodiesel using the commercial CRL preparations, mixtures of isozymes. In the present study, four liquid recombinant CRL isozymes (CRL1-CRL4) were investigated to convert various non-edible oils into biodiesel. The results showed that recombinant CRL2 and CRL4 exhibited superior catalytic efficiencies for producing fatty acid methyl ester (FAME) from Jatropha curcas seed oil. A maximum 95.3% FAME yield was achieved using CRL2 under the optimal conditions (50 wt% water, an initial 1 equivalent of methanol feeding, and an additional 0.5 equivalents of methanol feeding at 24h for a total reaction time of 48 h at 37 °C). We concluded that specific recombinant CRL isozymes could be excellent biocatalysts for the biodiesel production from low-cost crude Jatropha oil.
authors.affiliation: Department of Life Science, National Taiwan Normal University, Taipei, Taiwan.
authors.forename: Ting-Chun
authors.initials: TC
authors.lastname: Kuo
authors.affiliation: Department of Biological Science and Technology, I-Shou University, Kaohsiung, Taiwan.
authors.forename: Jei-Fu
authors.initials: JF
authors.lastname: Shaw
authors.affiliation: Department of Life Science, National Taiwan Normal University, Taipei, Taiwan. Electronic address: gclee@ntnu.edu.tw.
authors.forename: Guan-Chiun
authors.initials: GC
authors.lastname: Lee
mesh_terms: D056804:Biofuels / Q000382:microbiology*; D001709:Biotechnology / Q000379:methods; D002175:Candida / Q000201:enzymology*; D002384:Catalysis; D004951:Esterification; D005227:Fatty Acids / Q000737:chemistry; D007527:Isoenzymes / Q000737:chemistry*; D031286:Jatropha / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*
chemical_list: D056804:Biofuels; D005227:Fatty Acids; D007527:Isoenzymes; D010938:Plant Oils; D008049:Lipase; D000432:Methanol
keywords: Biodiesel; Candida rugosa lipase (CRL) isozyme; Crude seed oil; Jatropha curcas; Soluble lipase