title: Impact of Cu(2+) ions on the structure of colistin and cell-free system nucleic acid degradation.
abstract: Colistin and transition metal ions are commonly used as feed additives for livestock animals. This work presents the results of an analysis of combined potentiometric and spectroscopic (UV-vis, EPR, CD, NMR) data which lead to conclude that colistin is able to effectively chelate copper(II) ions. In cell-free system the oxidative activity of the complex manifests itself in the plasmid DNA destruction with simultaneous generation of reactive OH species, when accompanied by hydrogen peroxide or ascorbic acid. The degradation of RNA occurs most likely via a hydrolytic mechanism not only for complexed compound but also colistin alone. Therefore, huge amounts of the used antibiotic for nontherapeutic purposes might have a potential influence on livestock health.
authors.affiliation: Faculty of Chemistry, University of Wrocław, Joliot-Curie 14, 50-383 Wrocław, Poland. Electronic address: kamila.stokowa@chem.uni.wroc.pl.
authors.forename: Kamila
authors.initials: K
authors.lastname: Stokowa-Sołtys
authors.affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznań, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Kasprowicz
authors.affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznań, Poland.
authors.forename: Jan
authors.initials: J
authors.lastname: Wrzesiński
authors.affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznań, Poland.
authors.forename: Jerzy
authors.initials: J
authors.lastname: Ciesiołka
authors.affiliation: Department of Biotechnology, Chemistry and Pharmacy Via Aldo Moro, 2-53100 Siena, Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Gaggelli
authors.affiliation: Department of Biotechnology, Chemistry and Pharmacy Via Aldo Moro, 2-53100 Siena, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Gaggelli
authors.affiliation: Department of Biotechnology, Chemistry and Pharmacy Via Aldo Moro, 2-53100 Siena, Italy.
authors.forename: Gianni
authors.initials: G
authors.lastname: Valensin
authors.affiliation: Faculty of Chemistry, University of Wrocław, Joliot-Curie 14, 50-383 Wrocław, Poland. Electronic address: malgorzata.jezowska-bojczuk@chem.uni.wroc.pl.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Jeżowska-Bojczuk
mesh_terms: D002474:Cell-Free System / Q000187:drug effects; D002942:Circular Dichroism; D003091:Colistin / Q000737:chemistry*; D056831:Coordination Complexes; D003300:Copper / Q000737:chemistry* / Q000494:pharmacology*; D007477:Ions / Q000494:pharmacology; D015394:Molecular Structure; D009696:Nucleic Acids / Q000737:chemistry*; D010084:Oxidation-Reduction / Q000187:drug effects
chemical_list: D056831:Coordination Complexes; D007477:Ions; D009696:Nucleic Acids; D003300:Copper; D003091:Colistin
keywords: Colistin; Copper(II) complex; DNA and RNA degradation; Oxidative and hydrolytic properties