title: Natural polysaccharides promote chondrocyte adhesion and proliferation on magnetic nanoparticle/PVA composite hydrogels.
abstract: This paper aims to investigate the synergistic effects of natural polysaccharides and inorganic nanoparticles on cell adhesion and growth on intrinsically cell non-adhesive polyvinyl alcohol (PVA) hydrogels. Previously, we have demonstrated that Fe2O3 and hydroxyapatite (nHAP) nanoparticles are effective in increasing osteoblast growth on PVA hydrogels. Herein, we blended hyaluronic acid (HA) and chondroitin sulfate (CS), two important components of cartilage extracellular matrix (ECM), with Fe2O3/nHAP/PVA hydrogels. The presence of these natural polyelectrolytes dramatically increased the pore size and the equilibrium swelling ratio (ESR) while maintaining excellent compressive strength of hydrogels. Chondrocytes were seeded and cultured on composite PVA hydrogels containing Fe2O3, nHAP and Fe2O3/nHAP hybrids and Fe2O3/nHAP with HA or CS. Confocal laser scanning microscopy (CLSM) and cell counting kit-8 (CCK-8) assay consistently confirmed that the addition of HA or CS promotes chondrocyte adhesion and growth on PVA and composite hydrogels. Particularly, the combination of HA and CS exhibited further promotion to cell adhesion and proliferation compared with any single polysaccharide. The results demonstrated that the magnetic composite nanoparticles and polysaccharides provided synergistic promotion to cell adhesion and growth. Such polysaccharide-augmented composite hydrogels may have potentials in biomedical applications.
authors.affiliation: Polymers and Composites Division, Ningbo Institute of Materials Technology & Engineering, Chinese Academy of Sciences, Ningbo 315201, PR China.
authors.forename: Ruixia
authors.initials: R
authors.lastname: Hou
authors.affiliation: Polymers and Composites Division, Ningbo Institute of Materials Technology & Engineering, Chinese Academy of Sciences, Ningbo 315201, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Nie
authors.affiliation: Polymers and Composites Division, Ningbo Institute of Materials Technology & Engineering, Chinese Academy of Sciences, Ningbo 315201, PR China.
authors.forename: Gaolai
authors.initials: G
authors.lastname: Du
authors.affiliation: School of Materials Science and Engineering, Xiamen University, Xiamen 361005, PR China.
authors.forename: Xiaopeng
authors.initials: X
authors.lastname: Xiong
authors.affiliation: Polymers and Composites Division, Ningbo Institute of Materials Technology & Engineering, Chinese Academy of Sciences, Ningbo 315201, PR China. Electronic address: fujun@nimte.ac.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Fu
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D020100:Hydrogels; D008280:Magnetics; D008854:Microscopy, Electron; D011134:Polysaccharides / Q000494:pharmacology*; D011142:Polyvinyl Alcohol / Q000737:chemistry*
chemical_list: D020100:Hydrogels; D011134:Polysaccharides; D011142:Polyvinyl Alcohol
keywords: Chondrocytes; Hydrogel; Hydroxyapatite; Natural polysaccharide; PVA