title: Whole genome microarray analysis in non-small cell lung cancer.
abstract: Lung cancer is a serious health problem, since it is one of the leading causes for death worldwide. Molecular-cytogenetic studies could provide reliable data about genetic alterations which could be related to disease pathogenesis and be used for better prognosis and treatment strategies. We performed whole genome oligonucleotide microarray-based comparative genomic hybridization in 10 samples of non-small cell lung cancer. Trisomies were discovered for chromosomes 1, 13, 18 and 20. Chromosome arms 5p, 7p, 11q, 20q and Хq were affected by genetic gains, and 1p, 5q, 10q and 15q, by genetic losses. Microstructural (<5 Mbp) genomic aberrations were revealed: gains in regions 7p (containing the epidermal growth factor receptor gene) and 12p (containingKRAS) and losses in 3p26 and 4q34. Based on high amplitude of alterations and small overlapping regions, new potential oncogenes may be suggested:NBPF4(1p13.3);ETV1,AGR3andTSPAN13(7p21.3-7p21.1);SOX5andFGFR1OP2(12p12.1-12p11.22);GPC6(13q32.1). Significant genetic losses were assumed to contain potential tumour-suppressor genes:DPYD(1p21.3);CLDN22,CLDN24,ING2,CASP3,SORBS2(4q34.2-q35.1);DEFB(8p23.1). Our results complement the picture of genomic characterization of non-small cell lung cancer.
authors.affiliation: Department of Medical Genetics, Faculty of Medicine, Medical University of Sofia , Sofia , Bulgaria.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Al Zeyadi
authors.affiliation: Department of Medical Genetics, Faculty of Medicine, Medical University of Sofia , Sofia , Bulgaria.
authors.forename: Ivanka
authors.initials: I
authors.lastname: Dimova
authors.affiliation: Department of Medical Genetics, Faculty of Medicine, Medical University of Sofia , Sofia , Bulgaria.
authors.forename: Vladislav
authors.initials: V
authors.lastname: Ranchich
authors.affiliation: Department of Medical Genetics, Faculty of Medicine, Medical University of Sofia , Sofia , Bulgaria.
authors.forename: Blaga
authors.initials: B
authors.lastname: Rukova
authors.affiliation: Department of Medical Genetics, Faculty of Medicine, Medical University of Sofia , Sofia , Bulgaria.
authors.forename: Desislava
authors.initials: D
authors.lastname: Nesheva
authors.affiliation: Department of Medical Genetics, Faculty of Medicine, Medical University of Sofia , Sofia , Bulgaria.
authors.forename: Zora
authors.initials: Z
authors.lastname: Hamude
authors.affiliation: Department of Medical Genetics, Faculty of Medicine, Medical University of Sofia , Sofia , Bulgaria.
authors.forename: Sevdalin
authors.initials: S
authors.lastname: Georgiev
authors.affiliation: Clinic of Thoracic Surgery, Medical University of Sofia , Sofia , Bulgaria.
authors.forename: Danail
authors.initials: D
authors.lastname: Petrov
authors.affiliation: Department of Medical Genetics, Faculty of Medicine, Medical University of Sofia , Sofia , Bulgaria.
authors.forename: Draga
authors.initials: D
authors.lastname: Toncheva
keywords: array CGH; non-small cell lung cancer; oncogenes; tumour-suppressor genes