title: Parallel donor genotyping for 46 selected blood group and 4 human platelet antigens using high-throughput MALDI-TOF mass spectrometry.
abstract: Most blood group antigens are defined by single nucleotide polymorphisms (SNPs). Highly accurate MALDI-TOF MS has proven its potential in SNP genotyping and was therefore chosen for blood donor oriented genotyping with high-throughput capability, e.g., 380 samples per day. The Select Module covers a total of 36 SNPs in two single-tube reactions, representative of 46 blood group and 4 human platelet antigens. Using this tool, confirmatory blood group typing for RhD, RhCE, Kell, Kidd, Duffy, MN, Ss, and selected rare antigens is performed on a routine basis.
authors.affiliation: Department of Molecular Diagnostics and Research (MOC), Swiss Red Cross Blood Transfusion Service Zurich, Rütistrasse 19, 8952, Schlieren, Switzerland.
authors.forename: Stefan
authors.initials: S
authors.lastname: Meyer
authors.forename: Nadine
authors.initials: N
authors.lastname: Trost
authors.forename: Beat M
authors.initials: BM
authors.lastname: Frey
authors.forename: Christoph
authors.initials: C
authors.lastname: Gassner
mesh_terms: D016824:Antigens, Human Platelet / Q000032:analysis / Q000235:genetics*; D001483:Base Sequence; D001789:Blood Group Antigens / Q000032:analysis / Q000235:genetics*; D004247:DNA / Q000032:analysis / Q000235:genetics; D005838:Genotype; D060005:Genotyping Techniques / Q000191:economics / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing / Q000191:economics / Q000379:methods; D006801:Humans; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000191:economics / Q000379:methods*
chemical_list: D016824:Antigens, Human Platelet; D001789:Blood Group Antigens; D004247:DNA