title: Establishment of Immortalized Mouse Bmp2 Knock-Out Dental Papilla Mesenchymal Cells Necessary for Study of Odontoblastic Differentiation and Odontogenesis.
abstract: Bmp2 is essential for dentin formation. Bmp2 cKO mice exhibited similar phenotype to dentinogenesis imperfecta, showing dental pulp exposure, hypomineralized dentin, and delayed odontoblast differentiation. As it is relatively difficult to obtain lot of primary Bmp2 cKO dental papilla mesenchymal cells and to maintain a long-term culture of these primary cells, availability of immortalized deleted Bmp2 dental papilla mesenchymal cells is critical for studying the underlying mechanism of Bmp2 signal in odontogenesis. In this study, our goal was to generate an immortalized deleted Bmp2 dental papilla mesenchymal (iBmp2(ko/ko)dp) cell line by introducing Cre recombinase and green fluorescent protein (GFP) into the immortalized mouse floxed Bmp2 dental papilla mesenchymal (iBmp2(fx/fx)dp) cells. iBmp2(ko/ko)dp cells were confirmed by GFP and PCR. The deleted Bmp2 cells exhibited slow cell proliferation rate and cell growth was arrested in G2 phase. Expression of tooth-related marker genes and cell differentiation were decreased in the deleted cells. Importantly, extracellular matrix remodeling was impaired in the iBmp2(ko/ko)dp cells as reflected by the decreased Mmp-9 expression. In addition, with exogenous Bmp2 induction, these cell differentiation and mineralization were rescued as well as extracellular matrix remodeling was enhanced. Therefore, we for the first time described establishment of iBmp(ko/ko) cells that are useful for study of mechanisms in regulating dental papilla mesenchymal cell lineages.
authors.affiliation: Department of Developmental Dentistry, The University of Texas Health Science Center at San Antonio, San Antonio, Texas.|State Key Laboratory of Military Stomatology, Department of Pediatric Dentistry, School of Stomatology, The Fourth Military Medical University, Xi-an, China.
authors.forename: Lian
authors.initials: L
authors.lastname: Wu
authors.affiliation: Department of Developmental Dentistry, The University of Texas Health Science Center at San Antonio, San Antonio, Texas.|Department of Anatomy, Histology and Embryology, Basic Medical College, Fujian Medical University, Fuzhou, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Developmental Dentistry, The University of Texas Health Science Center at San Antonio, San Antonio, Texas.
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Donly
authors.affiliation: Department of Developmental Dentistry, The University of Texas Health Science Center at San Antonio, San Antonio, Texas.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Wan
authors.affiliation: Department of Developmental Dentistry, The University of Texas Health Science Center at San Antonio, San Antonio, Texas.
authors.forename: Daoshu
authors.initials: D
authors.lastname: Luo
authors.affiliation: Department of Periodontics, The University of Texas Health Science Center at San Antonio, San Antonio, Texas.
authors.forename: Stephen E
authors.initials: SE
authors.lastname: Harris
authors.affiliation: Department of Oral/Maxillofacial Surgery, University of Alabama at Birmingham, School of Dentistry, Birmingham, Alabama.
authors.forename: Mary
authors.initials: M
authors.lastname: MacDougall
authors.affiliation: Department of Developmental Dentistry, The University of Texas Health Science Center at San Antonio, San Antonio, Texas.
authors.forename: Shuo
authors.initials: S
authors.lastname: Chen
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000096:biosynthesis / Q000235:genetics*; D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line; D019070:Cell Lineage; D049109:Cell Proliferation / Q000235:genetics; D003771:Dental Papilla / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D055786:Gene Knockout Techniques; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D051379:Mice; D009804:Odontoblasts / Q000166:cytology* / Q000378:metabolism; D009805:Odontogenesis / Q000235:genetics*; D014070:Tooth / Q000166:cytology / Q000254:growth & development / Q000378:metabolism
chemical_list: D055396:Bone Morphogenetic Protein 2