title: In vivo immunological activity of carboxymethylated-sulfated (1→3)-β-D-glucan from sclerotium of Poria cocos.
abstract: β-Glucans are one of the polysaccharides known as biological response modifiers extracted from the sclerotium of Poria cocos which has been used for several decades as Traditional Chinese Medicine. Due to its ability to activate immune system, it can be applied in chemotherapy after being chemically modified. In this study, sulfated (1→3)-β-D-glucan (S-P), carboxymethyl (1→3)-β-D-glucan (CMP), and carboxymethylated-sulfated (1→3)-β-D-glucan (S-CMP), which are (1→3)-β-D-glucan derivatives were synthesized. The current study was aimed to investigate in vivo potential immunological activity of S-CMP in mice. In addition, mice were separately treated with S-P, CMP and S-CMP to evaluate the relationship between single and multiple functional groups. Interestingly, S-CMP exhibited the best in vivo immunological activities and the highest inhibition rate against the implanted HepG2 tumor in BALB/c mice, with significant increase in serum hemolysin antibody titer, spleen antibody production as well as delayed type hypersensitivity compared with S-P and CMP. Furthermore, it was assumed that simultaneous introduction of carboxymethyl and sulfate groups also had great potential effect on antioxidant activity, as substantial decrease in malondialdehyde (MDA) content was remarked. Therefore, it may suggest that S-CMP has better immunological and anti-tumor effects on mice in vivo.
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology (Ministry of Education), College of Chemistry, Central China Normal University, Wuhan 430079, PR China.
authors.forename: Haili
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology (Ministry of Education), College of Chemistry, Central China Normal University, Wuhan 430079, PR China.
authors.forename: Jean Felix
authors.initials: JF
authors.lastname: Mukerabigwi
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology (Ministry of Education), College of Chemistry, Central China Normal University, Wuhan 430079, PR China.
authors.forename: Yuannian
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology (Ministry of Education), College of Chemistry, Central China Normal University, Wuhan 430079, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Han
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology (Ministry of Education), College of Chemistry, Central China Normal University, Wuhan 430079, PR China.
authors.forename: Telieke
authors.initials: T
authors.lastname: Jiayinaguli
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology (Ministry of Education), College of Chemistry, Central China Normal University, Wuhan 430079, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology (Ministry of Education), College of Chemistry, Central China Normal University, Wuhan 430079, PR China.
authors.forename: Lina
authors.initials: L
authors.lastname: Liu
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology (Ministry of Education), College of Chemistry, Central China Normal University, Wuhan 430079, PR China. Electronic address: caoyu@mail.ccnu.edu.cn.
authors.forename: Yu
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology (Ministry of Education), College of Chemistry, Central China Normal University, Wuhan 430079, PR China.
authors.forename: Renqiang
authors.initials: R
authors.lastname: Sun
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology (Ministry of Education), College of Chemistry, Central China Normal University, Wuhan 430079, PR China.
authors.forename: Xueying
authors.initials: X
authors.lastname: Huang
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood; D000917:Antibody Formation / Q000187:drug effects; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002236:Carbohydrate Conformation; D062610:Fungal Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D005936:Glucans / Q000737:chemistry / Q000494:pharmacology; D006460:Hemolysin Proteins / Q000276:immunology; D056945:Hep G2 Cells; D006801:Humans; D007155:Immunologic Factors / Q000302:isolation & purification / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008516:Medicine, Chinese Traditional; D008807:Mice, Inbred BALB C; D055441:Poria / Q000737:chemistry; D013154:Spleen / Q000187:drug effects; D023041:Xenograft Model Antitumor Assays
chemical_list: D000906:Antibodies; D000970:Antineoplastic Agents; D000975:Antioxidants; D062610:Fungal Polysaccharides; D005936:Glucans; D006460:Hemolysin Proteins; D007155:Immunologic Factors
keywords: Carboxymethylated-sulfated (1→3)-β-d-glucan; Immunological activity; In vivo