title: MMPs and angiogenesis affect the metastatic potential of a human vulvar leiomyosarcoma cell line.
abstract: Gynaecological leiomyosarcoma (gLMS) represent a heterogeneous group of soft tissue sarcoma, characterized by rare incidence, high aggressiveness and propensity to infiltrate secondary organs, poor prognosis and lethality, because of the lack of biological mechanisms that underlying their progression and effective pharmaceutical treatments. This study was focused on some of the aspects of progression and dissemination of a subtype of gLMS namely vulvar LMS (vLMS). We therefore used a vulvar LMS-derived cell line namely SK-LMS-1, coupled with in vitro and in vivo assays. We observed that SK-LMS-1 cells have a strong invasive capacity in vitro, through the activity of matrix metalloproteinases 2 and 9, while in vivo these cells induce a strong angiogenic response and disseminate to the chick embryo liver. Therefore, we postulate that metalloproteinases are involved in the spreading behaviour of SK-LMS-1. Further investigations are necessary to better understand the molecular and cellular machinery involved in the progression of this malignancy.
authors.affiliation: Department of Life Sciences, University of Parma, Parma, Italy.
authors.forename: Carlotta
authors.initials: C
authors.lastname: Alias
authors.affiliation: Surgical Pathology Unit, University Hospital of Parma, Parma, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Rocchi
authors.affiliation: Department of Basic Medical Sciences, Neurosciences and Sensory Organs, Section of Human Anatomy and Histology, University of Bari Medical School, National Cancer Institute "Giovanni Paolo II", Bari, Italy.
authors.forename: Domenico
authors.initials: D
authors.lastname: Ribatti
authors.affiliation: Surgical Pathology Unit, University Hospital of Parma, Parma, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Caraffi
authors.affiliation: Department of Life Sciences, University of Parma, Parma, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: D'Angelo
authors.affiliation: Department of Life Sciences, University of Parma, Parma, Italy.|Centre for Molecular and Translational Oncology (COMT), University of Parma, Parma, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Perris
authors.affiliation: Surgical Pathology Unit, University Hospital of Parma, Parma, Italy.|Centre for Molecular and Translational Oncology (COMT), University of Parma, Parma, Italy.
authors.forename: Domenica
authors.initials: D
authors.lastname: Mangieri
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000378:metabolism; D000818:Animals; D045744:Cell Line, Tumor; D002645:Chickens; D049033:Chorioallantoic Membrane / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D004338:Drug Combinations; D004789:Enzyme Activation; D005260:Female; D006801:Humans; D007797:Laminin / Q000378:metabolism; D007890:Leiomyosarcoma / Q000098:blood supply* / Q000201:enzymology* / Q000473:pathology; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D009389:Neovascularization, Pathologic / Q000201:enzymology*; D011509:Proteoglycans / Q000378:metabolism; D014846:Vulvar Neoplasms / Q000098:blood supply* / Q000201:enzymology* / Q000473:pathology
chemical_list: D043925:Angiogenesis Inducing Agents; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen; D020782:Matrix Metalloproteinases
keywords: angiogenesis; chicken CAM; human vulvar leiomyosarcoma; matrix metalloproteinases; tumour progression