title: Upregulation of a disintegrin and metalloprotease 8 is associated with progression and prognosis of patients with gastric cancer.
abstract: A disintegrin and metalloprotease 8 (ADAM8) is involved in the tumorigenesis of several types of solid tumors. However, its exact role in gastric cancer (GC) remains unclear. The aim of this study was to evaluate the clinical significance of ADAM8 in GC and to explore its biological effects on gastric carcinogenesis. In this study, quantitative reverse transcription-polymerase chain reaction, Western blotting, and immunohistochemical staining analysis revealed that ADAM8 messenger RNA expression was significantly upregulated in GC tissues compared with noncancerous tissues (P = 0.004), and that positive ADAM8 expression is much more common in tumor tissues compared with normal tissues (P < 0.001) and is correlated with T stage (P = 0.036), N stage (P = 0.048), vessel invasion (P = 0.002), and a shorter patient overall survival (P = 0.024). In vitro assay indicated that ADAM8 overexpression promoted cell growth and increased migration and invasion abilities by decreasing the p-p38/p-extracellular regulated protein kinases (p-ERK) ratio. In conclusion, ADAM8 promotes GC cell proliferation and invasion, and its expression is positively correlated with poor survival, indicating that it might be a promising target in GC therapy.
authors.affiliation: Department of Gastrointestinal Surgery, The Sixth Affiliated Hospital of Sun Yat-Sen University (Guangdong Gastrointestinal and Anal Hospital), Sun Yat-Sen University, Guangzhou, PR China; Guangdong Institute of Gastroenterology, Guangzhou, PR China.
authors.forename: Jintuan
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Gastrointestinal Surgery, The Sixth Affiliated Hospital of Sun Yat-Sen University (Guangdong Gastrointestinal and Anal Hospital), Sun Yat-Sen University, Guangzhou, PR China; Guangdong Institute of Gastroenterology, Guangzhou, PR China; Department of Colorectal Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Department of Ophthalmology, The First Affiliated Hospital of Sun Yat-Sen University, Sun Yat-Sen University, Guangzhou, PR China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Huo
authors.affiliation: Department of Medical Oncology, The Sixth Affiliated Hospital of Sun Yat-Sen University (Guangdong Gastrointestinal and Anal Hospital), Sun Yat-Sen University, Guangzhou, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Xiao
authors.affiliation: Guangdong Institute of Gastroenterology, Guangzhou, PR China.
authors.forename: Xinjuan
authors.initials: X
authors.lastname: Fan
authors.affiliation: Guangdong Institute of Gastroenterology, Guangzhou, PR China.
authors.forename: Zihuan
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Department of Gastrointestinal Surgery, The Sixth Affiliated Hospital of Sun Yat-Sen University (Guangdong Gastrointestinal and Anal Hospital), Sun Yat-Sen University, Guangzhou, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Gastrointestinal Surgery, The Sixth Affiliated Hospital of Sun Yat-Sen University (Guangdong Gastrointestinal and Anal Hospital), Sun Yat-Sen University, Guangzhou, PR China; Guangdong Institute of Gastroenterology, Guangzhou, PR China. Electronic address: yzlhlj7068@163.com.
authors.forename: Zuli
authors.initials: Z
authors.lastname: Yang
mesh_terms: D051722:ADAM Proteins / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism*; D049109:Cell Proliferation; D018450:Disease Progression; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D011379:Prognosis; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; D008565:Membrane Proteins; D051722:ADAM Proteins; C068490:ADAM8 protein, human