title: Simultaneous determination of four secoiridoid and iridoid glycosides in rat plasma by ultra performance liquid chromatography-tandem mass spectrometry and its application to a comparative pharmacokinetic study.
abstract: A simple, reliable and rapid ultra-performance liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous quantification of four secoiridoid (gentiopicroside, swertiamarin, sweroside) and iridoid glycosides (loganic acid), the bio-active ingredients in rat plasma. After liquid-liquid extraction, chromatographic separation was accomplished on a Shim-pack XR-ODS column with a mobile phase consisting of methanol and 0.1% formic acid in water. A triple quadrupole tandem mass spectrometry equipped with an electrospray ionization source was used as detector operating both in positive and negative ionization mode and operated by multiple-reaction monitoring scanning. The lower limits of quantitation were 0.25-30 ng/mL for all the analytes. Both intra-day and inter-day precision and accuracy of analytes were well within acceptance criteria (±15%). The mean extraction recoveries of analytes and internal standard (amygdalin) from rat plasma were all >71.4%. The validated method was successfully applied to a comparative pharmacokinetic study of four analytes in rat plasma between normal and arthritic rats after oral administration of Huo Luo Xiao Ling Dan and Gentiana macrophylla extract, respectively. Results showed significant differences in pharmacokinetic properties of the analytes among the different groups.
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wu
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Ai
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China.
authors.forename: Fenrong
authors.initials: F
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Ma
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China.
authors.forename: Qiaoxia
authors.initials: Q
authors.lastname: Bian
authors.affiliation: Mailman Research Center, McLean Hospital, Harvard Medical School, Boston, MA, United States.
authors.forename: David Y-W
authors.initials: DY
authors.lastname: Lee
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China.
authors.forename: Ronghua
authors.initials: R
authors.lastname: Dai
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001169:Arthritis, Experimental; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000493:pharmacokinetics; D057889:Iridoid Glycosides / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification / Q000493:pharmacokinetics; D016014:Linear Models; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D004365:Drugs, Chinese Herbal; D057889:Iridoid Glycosides; C547651:huo luo xiao ling dan
keywords: Huo Luo Xiao Ling Dan; UPLC-MS/MS; arthritis; comparative pharmacokinetics; rat plasma