title: The role of 8-OH-DPAT on the rat neuronal apoptosis after diffuse brain injury coupled with secondary brain injury.
abstract: The potential role of 8-hydroxy-2-(di-n-propylamino) tetralin (8-OH-DPAT) on rat neuronal apoptosis after diffuse brain injury (DBI) coupled with secondary brain injury (SBI) was investigated. One hundred and twelve adult male Wister rats weighing 305-355 g were randomly divided into four groups and received an intraperitoneal injection of 8-OH-DPAT (0.5 mg/kg) or an equal volume of normal saline. Neurological severity score (NSS) was recorded and the injured extent was observed after hematoxylin-eosin (HE) staining. The neuronal cell apoptosis index and the expression of Bax and Bcl-2 were detected by TUNEL method and immunohistochemistry respectively. We found a higher NSS value for rats in the DBI + SBI groups compared with those in normal control and sham-operated control groups (P < 0.01). HE staining showed that 8-OH-DPAT treatment could alleviate the occurrence of injury in rats CA3 hippocampus and PFC. The neuronal apoptosis index decreased in the 8-OH-DPAT treatment group compared with the NS group (P< 0.05) and gradually increased at 6 h, reached the peak level at 72 h and still had a high performance at 168 h in not only CA3 hippocampus but also PFC. Expression of Bax and Bcl-2 increased after DBI + SBI, however, with 8-OH-DPAT treatment Bcl-2 expression increased while Bax expression decreased. 8-OH-DPAT had an inhibitory effect on the rat neuronal apoptosis in CA3 hippocampus and PFC after DBI coupled with SBI.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Zhenquan
authors.initials: Z
authors.lastname: Song
authors.forename: Guobiao
authors.initials: G
authors.lastname: Liang
authors.forename: Wei
authors.initials: W
authors.lastname: Lei
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Wang
authors.forename: Enzhi
authors.initials: E
authors.lastname: Liu
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Li
authors.forename: Hongbo
authors.initials: H
authors.lastname: Liu
mesh_terms: D017371:8-Hydroxy-2-(di-n-propylamino)tetralin / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001930:Brain Injuries / Q000188:drug therapy / Q000473:pathology*; D056654:CA3 Region, Hippocampal / Q000187:drug effects / Q000473:pathology; D008297:Male; D009474:Neurons / Q000187:drug effects*; D017397:Prefrontal Cortex / Q000187:drug effects / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017366:Serotonin Receptor Agonists / Q000494:pharmacology*; D016896:Treatment Outcome; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: C494828:Bax protein, rat; D019253:Proto-Oncogene Proteins c-bcl-2; D017366:Serotonin Receptor Agonists; D051028:bcl-2-Associated X Protein; D017371:8-Hydroxy-2-(di-n-propylamino)tetralin