title: Preparation and characterization of (PCL-crosslinked-PEG)/hydroxyapatite as bone tissue engineering scaffolds.
abstract: In this study, interconnected porous bioactive scaffolds were synthesized for bone tissue engineering. At the first step, poly( ɛ-caprolactone) (PCL) diols were diacrylated with acryloyl chloride. Then, the scaffolds were synthesized by radical crosslinking reaction of PCL and poly(ethyleneglycol) (PEG) diacrylates in the presence of hydroxyapatite (HA) particles. Morphological, swelling, thermal, and mechanical characteristics as well as degradability of the scaffolds were investigated. Results showed that increasing the ratio of PEG to PCL led to significant increase of swelling ratio and degradation rate, and decrease of crystallinity and compressive modulus of the networks, respectively. It was found that the incorporation of HA particles with the polymer matrices resulted in an augmented crystallinity, a decreased swelling ratio, and also a significantly increased compressive modulus of the networks. Cytocompatability and osteoconductivity of the scaffolds were assessed by MTT and alkaline phosphatase (ALP) assays, respectively. The results confirmed the cytocompatible nature of PCL/PEG/HA scaffolds with no toxicity. MG-63 cells attached and spread on the pore walls offered by the scaffolds. PCL/PEG/HA scaffolds compared with PCL/PEG ones showed higher ALP activity. Thus, the results indicated that the PCL/PEG/HA scaffolds have the potential of being used as promising substrates in bone tissue engineering.
authors.affiliation: Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Narjes
authors.initials: N
authors.lastname: Koupaei
authors.affiliation: Department of Biomedical Engineering, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran.
authors.forename: Akbar
authors.initials: A
authors.lastname: Karkhaneh
authors.affiliation: Department of Animal and Marine Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
authors.forename: Morteza
authors.initials: M
authors.lastname: Daliri Joupari
mesh_terms: D018786:Bone Substitutes / Q000737:chemistry*; D001842:Bone and Bones / Q000166:cytology*; D002448:Cell Adhesion; D002460:Cell Line; D002470:Cell Survival; D017886:Durapatite / Q000737:chemistry*; D006801:Humans; D011091:Polyesters / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D016062:Porosity; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D018786:Bone Substitutes; D011091:Polyesters; C437167:poly(ethylene glycol)diacrylate; C016240:polycaprolactone; D011092:Polyethylene Glycols; D017886:Durapatite
keywords: biodegradable porous scaffolds; bone tissue engineering; hydroxyapatite; polycaprolactone; polyethylene glycol