title: The molecular mechanisms of sodium metabisulfite on the expression of K ATP and L-Ca2+ channels in rat hearts.
abstract: Sodium metabisulfite (SMB) is used as an antioxidant and antimicrobial agent in a variety of drugs and foods. However, there are few reported studies about its side effects. This study is to investigate the SMB effects on the expression of ATP-sensitive K(+) (KATP) and L-type calcium (L-Ca(2+)) channels in rat hearts. The results show that the mRNA and protein levels of the KATP channel subunits Kir6.2 and SUR2A were increased by SMB; on the contrary, SMB at 520 mg/kg significantly decreased the expression of the L-Ca(2+) channel subunits Cav1.2 and Cav1.3. This suggests that SMB can activate the expression of KATP channel by increasing the mRNA and protein levels of Kir6.2 and SUR2A, while it inhibits the expression of L-Ca(2+) channels by decreasing the mRNA and protein levels of Cav1.2 and Cav1.3 in rat hearts. Therefore, the molecular mechanism of the SMB effect on rat hearts might be related to the increased expression of KATP channels and the decreased expression of L-Ca(2+) channels.
authors.affiliation: Institute of Environmental Medicine and Toxicology, Institute of Environmental Science, Shanxi University, Taiyuan 030006, China. Electronic address: qxzhang@sxu.edu.cn.
authors.forename: Quanxi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Institute of Environmental Medicine and Toxicology, Institute of Environmental Science, Shanxi University, Taiyuan 030006, China.
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Institute of Environmental Medicine and Toxicology, Institute of Environmental Science, Shanxi University, Taiyuan 030006, China.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Institute of Environmental Medicine and Toxicology, Institute of Environmental Science, Shanxi University, Taiyuan 030006, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Tian
authors.affiliation: Institute of Environmental Medicine and Toxicology, Institute of Environmental Science, Shanxi University, Taiyuan 030006, China.
authors.forename: Ziqiang
authors.initials: Z
authors.lastname: Meng
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000633:toxicity; D000975:Antioxidants / Q000633:toxicity; D015220:Calcium Channels / Q000235:genetics / Q000378:metabolism; D020746:Calcium Channels, L-Type / Q000235:genetics / Q000378:metabolism; D006321:Heart / Q000187:drug effects; D008297:Male; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D024661:Potassium Channels, Inwardly Rectifying / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D017208:Rats, Wistar; D013447:Sulfites / Q000633:toxicity*; D064233:Sulfonylurea Receptors / Q000235:genetics / Q000378:metabolism; D000099161:Kcnj11 Channel
chemical_list: D000890:Anti-Infective Agents; D000975:Antioxidants; D015220:Calcium Channels; D020746:Calcium Channels, L-Type; D024661:Potassium Channels, Inwardly Rectifying; D012333:RNA, Messenger; D013447:Sulfites; D064233:Sulfonylurea Receptors; D000099161:Kcnj11 Channel; C577512:Abcc9 protein, rat; C419385:L-type calcium channel alpha(1C); C093712:Cacna1d protein, rat; C005200:sodium metabisulfite
keywords: Cardiovascular disease; Food additives; Heart; K(ATP) channels; L-Ca(2+) channels; Molecular mechanism; Negative inotropic effects; Preservative; Sodium metabisulfite; Ventricular cardiomyocytes