title: Concentrating genomic length DNA in a microfabricated array.
abstract: We demonstrate that a microfabricated bump array can concentrate genomic-length DNA molecules efficiently at continuous, high flow velocities, up to 40 μm/s, if the single-molecule DNA globule has a sufficiently large shear modulus. Increase in the shear modulus is accomplished by compacting the DNA molecules to minimal coil size using polyethylene glycol (PEG) derived depletion forces. We map out the sweet spot, where concentration occurs, as a function of PEG concentration and flow speed using a combination of theoretical analysis and experiment. Purification of DNA from enzymatic reactions for next-generation DNA-sequencing libraries will be an important application of this development.
authors.affiliation: Princeton Institute for Science and Technology of Materials (PRISM), Princeton, New Jersey 08540, USA.|Department of Electrical Engineering, Princeton University, Princeton, New Jersey 08544, USA.
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Sage Science, Inc., Beverly, Massachusetts 01915, USA.
authors.forename: Ezra S
authors.initials: ES
authors.lastname: Abrams
authors.affiliation: Sage Science, Inc., Beverly, Massachusetts 01915, USA.
authors.forename: T Christian
authors.initials: TC
authors.lastname: Boles
authors.affiliation: Department of Micro- and Nanotechnology, Technical University of Denmark, DK-2800 Kongens Lyngby, Denmark.
authors.forename: Jonas N
authors.initials: JN
authors.lastname: Pedersen
authors.affiliation: Department of Micro- and Nanotechnology, Technical University of Denmark, DK-2800 Kongens Lyngby, Denmark.
authors.forename: Henrik
authors.initials: H
authors.lastname: Flyvbjerg
authors.affiliation: Princeton Institute for Science and Technology of Materials (PRISM), Princeton, New Jersey 08540, USA.|Department of Physics, Princeton University, Princeton, New Jersey 08544, USA.
authors.forename: Robert H
authors.initials: RH
authors.lastname: Austin
authors.affiliation: Princeton Institute for Science and Technology of Materials (PRISM), Princeton, New Jersey 08540, USA.|Department of Electrical Engineering, Princeton University, Princeton, New Jersey 08544, USA.
authors.forename: James C
authors.initials: JC
authors.lastname: Sturm
mesh_terms: D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D055616:Microtechnology; D009690:Nucleic Acid Conformation; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D011092:Polyethylene Glycols / Q000737:chemistry; D033081:Shear Strength
chemical_list: D011092:Polyethylene Glycols; D004247:DNA