title: Study of the relationship between mononuclear inflammatory infiltrate and Ki-67 and basement membrane and extracellular matrix protein expression in radicular cysts.
abstract: OBJECTIVE
  To evaluate the relationship between mononuclear inflammatory infiltrate and the expression of a proliferative immunomarker (Ki-67) as well as to evaluate basement membrane and extracellular matrix proteins (laminin and collagen type IV) in radicular cysts and dentigerous cysts (DC).
  
  
  METHODS
  Immunohistochemical analyses were performed in heavily inflamed radicular cysts (HIRC), slightly inflamed radicular cysts (SIRC) and DC (n = 20) using Ki-67 (Dako(®) , 1 : 50), anticollagen type IV (DBS(®) , 1 : 40) and antilaminin (DBS(®) , 1 : 20). The data were analysed using anova/Tukey's test (Ki-67) and Kruskal-Wallis/Dunn's test (collagen type IV and laminin) (P < 0.05).
  
  
  RESULTS
  The immunoexpression of Ki-67 was significantly greater in the SIRC group compared with the HIRC and DC (P = 0.0040). Likewise, the immunoexpression of collagen type IV in the basement membrane of the SIRC group was significantly more continuous (P = 0.0475) than in the HIRC group. DC had significantly less collagen type IV in extracellular matrix immunoexpression than HIRC and SIRC (P = 0.0246). Laminin was absent in the basement membrane in the SIRC and DC groups, and the extracellular matrix of the HIRC was weak and punctate.
  
  
  CONCLUSIONS
  The presence of inflammatory factors in the radicular cyst wall modified the expression of proliferation factors in the epithelial lining and the expression of collagen type IV and laminin in the basement membrane, but did not modify extracellular matrix behaviour in radicular cysts.
authors.affiliation: Department of Pathology and Forensic Medicine, School of Medicine, Federal University of Ceará, Fortaleza, Brazil.
authors.forename: R V C
authors.initials: RV
authors.lastname: Mourão
authors.affiliation: Division of Endodontics, School of Dentistry, Fortaleza University, Fortaleza, Brazil.
authors.forename: E C Pinheiro
authors.initials: EC
authors.lastname: Júnior
authors.affiliation: Division of Oral Pathology, School of Dentistry, Federal University of Ceará, Fortaleza, Brazil.
authors.forename: P G
authors.identifier: http://orcid.org/0000-0002-1513-9027
authors.initials: PG
authors.lastname: Barros Silva
authors.affiliation: Division of Oral Pathology, School of Dentistry, Fortaleza University, Fortaleza, Brazil.
authors.forename: E
authors.initials: E
authors.lastname: Turatti
authors.affiliation: Division of Oral Pathology, School of Dentistry, Federal University of Ceará, Fortaleza, Brazil.
authors.forename: M R L
authors.initials: MR
authors.lastname: Mota
authors.affiliation: Division of Oral Pathology, School of Dentistry, Federal University of Ceará, Fortaleza, Brazil.
authors.forename: A P N N
authors.initials: AP
authors.lastname: Alves
mesh_terms: D001485:Basement Membrane / Q000378:metabolism; D003094:Collagen; D024141:Collagen Type IV; D005109:Extracellular Matrix; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism*; D007797:Laminin; D011842:Radicular Cyst / Q000378:metabolism*
chemical_list: D024141:Collagen Type IV; D016326:Extracellular Matrix Proteins; D019394:Ki-67 Antigen; D007797:Laminin; D003094:Collagen
keywords: collagen type IV; inflammation; radicular cyst