title: Voxel-based morphometry of the marmoset brain: In vivo detection of volume loss in the substantia nigra of the MPTP-treated Parkinson's disease model.
abstract: Movement dysfunction in Parkinson's disease (PD) is caused by the degeneration of dopaminergic (DA) neurons in the substantia nigra (SN). Here, we established a method for voxel-based morphometry (VBM) and automatic tissue segmentation of the marmoset monkey brain using a 7-T animal scanner and applied the method to assess DA degeneration in a PD model, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated animals, with tyrosine-hydroxylase staining. The most significant decreases of local tissue volume were detected in the bilateral SN of MPTP-treated marmoset brains (-53.0% in right and -46.5% in left) and corresponded with the location of DA neurodegeneration found in histology (-65.4% in right). In addition to the SN, the decreases were also confirmed in the locus coeruleus, and lateral hypothalamus. VBM using 7-T MRI was effective in detecting volume loss in the SN of the PD-model marmoset. This study provides a potential basis for the application of VBM with ultra-high field MRI in the clinical diagnosis of PD. The developed method may also offer value in automatic whole-brain evaluation of structural changes for the marmoset monkey.
authors.affiliation: Department of Physiology, Keio University School of Medicine, Tokyo, Japan; Central Institute for Experimental Animals, Kawasaki, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hikishima
authors.affiliation: Central Institute for Experimental Animals, Kawasaki, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ando
authors.affiliation: Department of Physiology, Keio University School of Medicine, Tokyo, Japan; Central Institute for Experimental Animals, Kawasaki, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Komaki
authors.affiliation: Central Institute for Experimental Animals, Kawasaki, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kawai
authors.affiliation: Department of Physiology, Keio University School of Medicine, Tokyo, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Yano
authors.affiliation: Central Institute for Experimental Animals, Kawasaki, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Inoue
authors.affiliation: Central Institute for Experimental Animals, Kawasaki, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Itoh
authors.affiliation: Faculty of Radiological Technology, Fujita Health University School of Health Sciences, Toyoake, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Yamada
authors.affiliation: Department of Diagnostic Radiology, Keio University School of Medicine, Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Momoshima
authors.affiliation: Division of Regenerative Medicine, Jikei University School of Medicine, Tokyo, Japan.
authors.forename: H J
authors.initials: HJ
authors.lastname: Okano
authors.affiliation: Department of Physiology, Keio University School of Medicine, Tokyo, Japan; Laboratory for Marmoset Neural Architecture, Brain Science Institute RIKEN, Wako, Japan. Electronic address: hidokano@a2.keio.jp.
authors.forename: H
authors.initials: H
authors.lastname: Okano
mesh_terms: D000818:Animals; D002144:Callithrix / Q000033:anatomy & histology* / Q000378:metabolism; D020267:MPTP Poisoning / Q000378:metabolism / Q000473:pathology*; D008279:Magnetic Resonance Imaging / Q000295:instrumentation / Q000379:methods*; D008297:Male; D009929:Organ Size; D010363:Pattern Recognition, Automated / Q000379:methods; D013378:Substantia Nigra / Q000378:metabolism / Q000473:pathology*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D014446:Tyrosine 3-Monooxygenase
keywords: Callithrix jacchus; MPTP; Parkinson’s disease; VBM; common marmoset; substantia nigra