title: Vitis labrusca leaf extract prevents pentylenetetrazol-induced oxidative damage but not seizures in rats.
abstract: Epilepsy is a disorder of the central nervous system characterized by recurrent seizures. It is a very common disease in which approximately 30% of patients do not respond favorably to treatment with anticonvulsants. Oxidative stress is associated with neuronal damage arising from epileptic seizures. The present study investigated the possible anticonvulsant and antioxidant effects of a leaf extract of Vitis labrusca in an animal model of seizures induced by pentylenetetrazole (PTZ). The animals received injections of V. labrusca extract (10, 30 and 100 mg/kg) or vehicle and, 30 minutes later, they received an injection of PTZ, and were then observed for 30 minutes. The latency time and tonic&mdash;clonic seizure time were registered. Oxidative damage in lipids and proteins was quantified in the cerebellum, cerebral cortex and hippocampus. It was observed that the leaf extract were capable of reducing lipid peroxidation and protein oxidation caused by PTZ at all doses tested.
authors.affiliation: Universidade Federal do Rio Grande do Sul Department of Microbiology, Immunology and Parasitology Brazil adriana.simon&commat;ufrgs.br.
authors.forename: A S
authors.initials: AS
authors.lastname: Coitinho
authors.affiliation: Centro Universitario Metodista IPA Porto Alegre Brazil.
authors.forename: B M
authors.initials: BM
authors.lastname: da Costa
authors.affiliation: Centro Universitario Metodista IPA Porto Alegre Brazil.
authors.forename: M T
authors.initials: MT
authors.lastname: Dos Santos
authors.affiliation: Centro Universitario Metodista IPA Porto Alegre Brazil.
authors.forename: B
authors.initials: B
authors.lastname: Fank
authors.affiliation: Universidade Federal do Rio Grande do Su Interdisciplinary Department in Science and Technology Brazil.
authors.forename: C L
authors.initials: CL
authors.lastname: Hackmann
authors.affiliation: Universidade de Caxias do Sul Institute of Biotechnology Caxias do Sul Brazil.
authors.forename: M
authors.initials: M
authors.lastname: Salvador
authors.affiliation: Centro Universitario Metodista IPA Porto Alegre Brazil.
authors.forename: C
authors.initials: C
authors.lastname: Dani
mesh_terms: D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002531:Cerebellum / Q000503:physiopathology; D002540:Cerebral Cortex / Q000503:physiopathology; D004195:Disease Models, Animal; D006624:Hippocampus / Q000503:physiopathology; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D010433:Pentylenetetrazole; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012640:Seizures / Q000139:chemically induced / Q000188:drug therapy / Q000517:prevention & control*; D027843:Vitis / Q000378:metabolism*
chemical_list: D000927:Anticonvulsants; D000975:Antioxidants; D010936:Plant Extracts; D010433:Pentylenetetrazole