title: Microglial ROS production in an electrical rat post-status epilepticus model of epileptogenesis.
abstract: Reactive oxygen species and inflammatory signaling have been identified as pivotal pathophysiological factors contributing to epileptogenesis. Considering the development of combined anti-inflammatory and antioxidant treatment strategies with antiepileptogenic potential, a characterization of the time course of microglial reactive oxygen species generation during epileptogenesis is of major interest. Thus, we isolated microglia cells and analyzed the generation of reactive oxygen species by flow cytometric analysis in an electrical rat post-status epilepticus model. Two days post status epilepticus, a large-sized cell cluster exhibited a pronounced response with excessive production of reactive oxygen species upon stimulation with phorbol-myristate-acetate. Neither in the latency phase nor in the chronic phase with spontaneous seizures a comparable cell population with induction of reactive oxygen species was identified. We were able to demonstrate in the electrical rat post-status-epilepticus model, that microglial ROS generation reaches a peak after the initial insult, is only marginally increased in the latency phase, and returns to control levels during the chronic epileptic phase. The data suggest that a combination of anti-inflammatory and radical scavenging approaches might only be beneficial during a short time window after an epileptogenic brain insult.
authors.affiliation: Institute of Pharmacology, Toxicology, and Pharmacy, Ludwig-Maximilians-University, Munich, Germany.
authors.forename: Maruja L
authors.initials: ML
authors.lastname: Rettenbeck
authors.affiliation: Institute of Pharmacology, Toxicology, and Pharmacy, Ludwig-Maximilians-University, Munich, Germany.
authors.forename: Eva-Lotta
authors.initials: EL
authors.lastname: von Rüden
authors.affiliation: Department of Small Animal Medicine and Surgery, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: Silvia
authors.initials: S
authors.lastname: Bienas
authors.affiliation: Department of Small Animal Medicine and Surgery, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: Regina
authors.initials: R
authors.lastname: Carlson
authors.affiliation: Department of Small Animal Medicine and Surgery, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: Veronika M
authors.initials: VM
authors.lastname: Stein
authors.affiliation: Department of Small Animal Medicine and Surgery, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Tipold
authors.affiliation: Institute of Pharmacology, Toxicology, and Pharmacy, Ludwig-Maximilians-University, Munich, Germany. Electronic address: potschka@pharmtox.vetmed.uni-muenchen.de.
authors.forename: Heidrun
authors.initials: H
authors.lastname: Potschka
mesh_terms: D000818:Animals; D004558:Electric Stimulation; D005260:Female; D017628:Microglia / Q000378:metabolism*; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012008:Recurrence; D012640:Seizures / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology*; D013226:Status Epilepticus / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology*
chemical_list: D017382:Reactive Oxygen Species