title: Shift Work in Rats Results in Increased Inflammatory Response after Lipopolysaccharide Administration: A Role for Food Consumption.
abstract: The suprachiasmatic nucleus (SCN) drives circadian rhythms in behavioral and physiological variables, including the inflammatory response. Shift work is known to disturb circadian rhythms and is associated with increased susceptibility to develop disease. In rodents, circadian disruption due to shifted light schedules (jet lag) induced increased innate immune responses. To gain more insight into the influence of circadian disruption on the immune response, we characterized the inflammatory response in a model of rodent shift work and demonstrated that circadian disruption affected the inflammatory response to lipopolysaccharide (LPS) both in vivo and in vitro. Since food consumption is a main disturbing element in the shift work schedule, we also evaluated the inflammatory response to LPS in a group of rats that had no access to food during their working hours. Our results demonstrated that the shift work schedule decreased basal TNF-α levels in the liver but not in the circulation. Despite this, we observed that shift work induced increased cytokine response after LPS stimulation in comparison to control rats. Also, Kupffer cells (liver macrophages) isolated from shift work rats produced more TNF-α in response to in vitro LPS stimulation, suggesting important effects of circadian desynchronization on the functionality of this cell type. Importantly, the effects of shift work on the inflammatory response to LPS were prevented when food was not available during the working schedule. Together, these results show that dissociating behavior and food intake from the synchronizing drive of the SCN severely disturbs the immune response.
authors.affiliation: Departamento de Biología Celular y Fisiología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México.
authors.forename: Natalí N
authors.initials: NN
authors.lastname: Guerrero-Vargas
authors.affiliation: Departamento de Biología Celular y Fisiología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México.
authors.forename: Mara
authors.initials: M
authors.lastname: Guzmán-Ruiz
authors.affiliation: Departamento de Biología Celular y Fisiología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México.
authors.forename: Rebeca
authors.initials: R
authors.lastname: Fuentes
authors.affiliation: Departamento de Biología Celular y Fisiología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México.
authors.forename: Joselyn
authors.initials: J
authors.lastname: García
authors.affiliation: Facultad de Ciencias, Universidad Autónoma de San Luis Potosí, San Luis Potosí, México.
authors.forename: Roberto
authors.initials: R
authors.lastname: Salgado-Delgado
authors.affiliation: Departamento de Biología Celular y Fisiología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México.
authors.forename: María del Carmen
authors.initials: Mdel C
authors.lastname: Basualdo
authors.affiliation: Departamento de Anatomía, Facultad de Medicina, Universidad Nacional Autónoma de México, México.
authors.forename: Carolina
authors.initials: C
authors.lastname: Escobar
authors.affiliation: Laboratory of Chronopharmacology, Department of Physiology, Institute of Biosciences, University of São Paulo (USP), São Paulo, Brazil.
authors.forename: Regina P
authors.initials: RP
authors.lastname: Markus
authors.affiliation: Departamento de Biología Celular y Fisiología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México ruudbuijs@gmail.com.
authors.forename: Ruud M
authors.initials: RM
authors.lastname: Buijs
mesh_terms: D000818:Animals; D001683:Biological Clocks / Q000502:physiology; D002478:Cells, Cultured; D021081:Chronobiology Disorders / Q000276:immunology / Q000503:physiopathology*; D002940:Circadian Rhythm; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D004435:Eating; D007249:Inflammation / Q000209:etiology*; D007728:Kupffer Cells / Q000276:immunology / Q000378:metabolism; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000276:immunology*; D008099:Liver / Q000276:immunology / Q000378:metabolism; D008297:Male; D017440:Photoperiod; D017208:Rats, Wistar; D013493:Suprachiasmatic Nucleus / Q000502:physiology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014940:Work Schedule Tolerance
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha
keywords: Kupffer cells; LPS; circadian desynchronization; cytokine; food; liver; shift work; suprachiasmatic nucleus