title: Regulatory role of B-cell maturation antigen on the toxic effect of chromium ions on human SaOS-2 osteoblasts.
abstract: Metal prostheses of artificial joints undergo wear, producing numerous metal particles and ions, such as Cr3+. Cr3+is considered a key factor leading to aseptic loosening. Many studies focus on the effect of Cr3+on osteoblasts; however, little is known about the effect of Cr3+on the B-cell maturation antigen (BCMA) in the osteoblasts. In this study, we first demonstrated the BCMA expressed in human SaOS-2 osteoblasts through reverse transcriptase-PCR, Western blot, and immunocytochemical analyses. Cr3+decreased alkaline phosphatase (ALP), osteocalcin (OC), cell mineralization, and collagen type I mRNA and protein expression. Moreover, Cr3+has an inhibitive effect on the expression of the BCMA in human SaOS-2 osteoblasts. However, after we upregulated the expression of the BCMA, ALP, OC, cell mineralization, and collagen type I mRNA and protein expression were increased. Overall, this study demonstrates that the BCMA is involved in human SaOS-2 osteoblast osteogenetic metabolism and plays a regulatory role on the toxic effect of chromium ions on human SaOS-2 osteoblasts.
authors.affiliation: Department of Orthopedics, Changhai Hospital Affiliated to the Second Military Medical University, Yangpu, Shanghai, People's Republic of China.
authors.forename: D H
authors.initials: DH
authors.lastname: Li
authors.affiliation: Department of Orthopedics, Changhai Hospital Affiliated to the Second Military Medical University, Yangpu, Shanghai, People's Republic of China.
authors.forename: Q
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Department of Biochemistry and Molecular Biology, Second Military Medical University, Yangpu, Shanghai, People's Republic of China.
authors.forename: J S
authors.initials: JS
authors.lastname: Zhou
authors.affiliation: Department of Biochemistry and Molecular Biology, Second Military Medical University, Yangpu, Shanghai, People's Republic of China.
authors.forename: Z W
authors.initials: ZW
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry and Molecular Biology, Second Military Medical University, Yangpu, Shanghai, People's Republic of China.
authors.forename: M Y
authors.initials: MY
authors.lastname: Miao
authors.affiliation: Department of Biochemistry and Molecular Biology, Second Military Medical University, Yangpu, Shanghai, People's Republic of China.
authors.forename: S S
authors.initials: SS
authors.lastname: Yang
authors.affiliation: Department of Orthopedics, Changhai Hospital Affiliated to the Second Military Medical University, Yangpu, Shanghai, People's Republic of China.
authors.forename: W D
authors.initials: WD
authors.lastname: Xu
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D053301:B-Cell Maturation Antigen / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002857:Chromium / Q000633:toxicity*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D010012:Osteogenesis / Q000187:drug effects* / Q000235:genetics
chemical_list: D053301:B-Cell Maturation Antigen; D002857:Chromium; D000469:Alkaline Phosphatase
keywords: BCMA; chromium ion; osteogenetic activity