title: The Effects of Lactobacillus acidophilus on the Intestinal Smooth Muscle Contraction through PKC/MLCK/MLC Signaling Pathway in TBI Mouse Model.
abstract: Clinical studies have shown that probiotics influence gastrointestinal motility. However, the molecular mechanisms by which probiotic Lactobacillus modulates intestinal motility in traumatic brain injury (TBI) mouse model have not been explored. In the present study, we provided evidence showing that treatment of TBI mice with Lactobacillus acidophilus significantly improved the terminal ileum villus morphology, restored the impaired interstitial cells of Cajal (ICC) and the disrupted ICC networks after TBI, and prevented TBI-mediated inhibition of contractile activity in intestinal smooth muscle. Mechanistically, the decreased concentration of MLCK, phospho-MLC20 and phospho-MYPT1 and increased concentration of MLCP and PKC were observed after TBI, and these events mediated by TBI were efficiently prevented by Lactobacillus acidophilus application. These findings may provide a novel mechanistic basis for the application of Lactobacillus acidophilus in the treatment of TBI.
authors.affiliation: School of Nursing, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Sun
authors.affiliation: School of Nursing, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Hu
authors.affiliation: School of Nursing, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Fang
authors.affiliation: School of Nursing, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Zhu
authors.affiliation: College of Pharmacy, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Gao
authors.affiliation: School of Nursing, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Jingci
authors.initials: J
authors.lastname: Zhu
mesh_terms: D000818:Animals; D001930:Brain Injuries / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology / Q000503:physiopathology; D002452:Cell Count; D004195:Disease Models, Animal; D056885:Interstitial Cells of Cajal / Q000378:metabolism / Q000473:pathology; D007421:Intestine, Small / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology*; D007779:Lactobacillus acidophilus / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009119:Muscle Contraction; D009130:Muscle, Smooth / Q000473:pathology / Q000503:physiopathology*; D018994:Myosin Light Chains / Q000378:metabolism; D009219:Myosin-Light-Chain Kinase / Q000378:metabolism; D043263:Myosin-Light-Chain Phosphatase; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D018994:Myosin Light Chains; D011493:Protein Kinase C; D009219:Myosin-Light-Chain Kinase; D043263:Myosin-Light-Chain Phosphatase; C510645:Ppp1r12a protein, mouse