title: [The experience of application of test system "TB-Biochip" in the Tambov oblast].
abstract: The analysis was implemented concerning application of biological chips technique developed by the V A. Engelgardt institute of molecular biology--"TB-Biochip" designed for detecting DNA agent of tuberculosis in diagnostic samples of human respiratory organs and establishing its medicinal sensitivity to rifampicin and isoniazid. It is demonstrated that "TB-Biochip" is a sensitive and highly specific method of detecting medicinal sensitivity to main anti-tuberculosis pharmaceuticals. At that, analysis time amount to less than 72 hours. The comparative analysis was carried out concerning results of detection of medicinal sensitivity of mycobacteria by two express techniques--TB-Biochip (Biochip-IMB) and BACTECTM MGITTM 960 ("Becton Dickonson"). The data obtained by two techniques matched in 97.6%for rifampicin and in 92.1% for isoniazid.
authors.forename: E V
authors.initials: EV
authors.lastname: Manaenkova
authors.forename: A A
authors.initials: AA
authors.lastname: Savin
mesh_terms: D004269:DNA, Bacterial / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology; D046228:Microarray Analysis / Q000379:methods*; D008826:Microbial Sensitivity Tests; D009154:Mutation / Q000187:drug effects; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D012293:Rifampin / Q000494:pharmacology; D012426:Russia; D013183:Sputum / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000235:genetics / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D007538:Isoniazid; D012293:Rifampin