title: Surface modifications for enhanced enzyme immobilization and improved electron transfer of PQQ-dependent glucose dehydrogenase anodes.
abstract: Pyrroloquinoline quinone dependent soluble glucose dehydrogenase (PQQ-sGDH) enzymatic MWCNT electrodes were p roduced using 1-pyrenecarboxylic acid (PCA) activated through carbodiimide functionalization and 1-Pyrenebutyric acid N-hydroxysuccinimide ester (PBSE) as tethering agents. At 600 mV potential, the current density generated by the activated-PCA tethered PQQ-sGDH anode was significantly greater than the current density generated by the untethered PQQ-sGDH and PBSE tethered anodes, and performance was nearly identical to the performance of a covalently bound PQQ-sGDH anode. A technique for covalently bonding heme-b (hemin), a natural quinohemoprotein porphyrin redox cofactor, to carbon nanotubes modified with arylamine groups is reported. The resulting performance of the covalently bound hemin PQQ-sGDH anode is considerably higher than that of any other PQQ-sGDH anodes tested.
authors.affiliation: Department of Chemical and Biological Engineering University of New Mexico, 1 University of New Mexico, Albuquerque, NM 87131, USA.
authors.forename: Ryan J
authors.initials: RJ
authors.lastname: Lopez
authors.affiliation: Department of Chemical and Biological Engineering University of New Mexico, 1 University of New Mexico, Albuquerque, NM 87131, USA.
authors.forename: Sofia
authors.initials: S
authors.lastname: Babanova
authors.affiliation: Department of Chemical and Biological Engineering University of New Mexico, 1 University of New Mexico, Albuquerque, NM 87131, USA.
authors.forename: Kateryna
authors.initials: K
authors.lastname: Artyushkova
authors.affiliation: Department of Chemical and Biological Engineering University of New Mexico, 1 University of New Mexico, Albuquerque, NM 87131, USA.
authors.forename: Plamen
authors.initials: P
authors.lastname: Atanassov
mesh_terms: D004566:Electrodes; D004579:Electron Transport; D004800:Enzymes, Immobilized / Q000737:chemistry*; D042843:Glucose 1-Dehydrogenase / Q000378:metabolism*; D056951:Photoelectron Spectroscopy; D013499:Surface Properties
chemical_list: D004800:Enzymes, Immobilized; D042843:Glucose 1-Dehydrogenase
keywords: Electron transfer; Enzymatic electrode; Immobilization; Redox chemistry; Voltammetry