title: Fatty acid binding protein 7 and n-3 poly unsaturated fatty acid supply in early rat brain development.
abstract: Fatty acid binding protein 7 (FABP7), abundant in the embryonic brain, binds with the highest affinity to docosahexaenoic acid (DHA) and is expressed in the early stages of embryogenesis. Here, we have examined the consequences of the exposure to different DHA levels and of the in utero depletion of FABP7 on early rat brain development. Neurodevelopment was evaluated through the contents of two proteins, connexin 43 (Cx43) and cyclin-dependent kinase 5 (CDK5), both involved in neuroblast proliferation, differentiation, and migration. The dams were fed with diets presenting different DHA contents, from deficiency to supplementation. DHA brain embryos contents already differed at embryonic day 11.5 and the differences kept increasing with time. Cx43 and CDK5 contents were positively associated with the brain DHA levels. When FABP7 was depleted in vivo by injections of siRNA in the telencephalon, the enhancement of the contents of both proteins was lost in supplemented animals, but FABP7 depletion did not modify phospholipid compositions regardless of the diets. Thus, FABP7 is a necessary mediator of the effect of DHA on these proteins synthesis, but its role in DHA uptake is not critical, although FABP7 is localized in phospholipid-rich areas. Our study shows that high contents of DHA associated with FABP7 are necessary to promote early brain development, which prompted us to recommend DHA supplementation early in pregnancy.
authors.affiliation: Département Alimentation Humaine, INRA, Unité De Nutrition Et Régulation Lipidique Des Fonctions Cérébrales (UR 909), F-78352, Jouy-en-Josas Cedex, France.
authors.forename: Elise
authors.initials: E
authors.lastname: Maximin
authors.affiliation: Département Alimentation Humaine, INRA, Unité De Nutrition Et Régulation Lipidique Des Fonctions Cérébrales (UR 909), F-78352, Jouy-en-Josas Cedex, France.
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Langelier
authors.affiliation: Département Alimentation Humaine, INRA, Unité De Nutrition Et Régulation Lipidique Des Fonctions Cérébrales (UR 909), F-78352, Jouy-en-Josas Cedex, France.
authors.forename: Josiane
authors.initials: J
authors.lastname: Aïoun
authors.affiliation: Département Physiologie Animale, Unité De Biologie Du Développement Et De La Reproduction (UMR 1198), F-78352, Jouy-en-Josas Cedex, France.
authors.forename: Kaïs H
authors.initials: KH
authors.lastname: Al-Gubory
authors.affiliation: Département Alimentation Humaine, INRA, Unité De Nutrition Et Régulation Lipidique Des Fonctions Cérébrales (UR 909), F-78352, Jouy-en-Josas Cedex, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Bordat
authors.affiliation: Département Alimentation Humaine, INRA, Unité De Nutrition Et Régulation Lipidique Des Fonctions Cérébrales (UR 909), F-78352, Jouy-en-Josas Cedex, France.
authors.forename: Monique
authors.initials: M
authors.lastname: Lavialle
authors.affiliation: Département Alimentation Humaine, INRA, Unité De Nutrition Et Régulation Lipidique Des Fonctions Cérébrales (UR 909), F-78352, Jouy-en-Josas Cedex, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Heberden
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D001923:Brain Chemistry; D004032:Diet; D004281:Docosahexaenoic Acids / Q000494:pharmacology*; D004622:Embryo, Mammalian; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins / Q000378:metabolism*; D005260:Female; D055785:Gene Knockdown Techniques; D007150:Immunohistochemistry; D018811:Maternal Exposure; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D055495:Neurogenesis / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: C490174:Fabp7 protein, rat; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D009419:Nerve Tissue Proteins; D004281:Docosahexaenoic Acids
keywords: brain development; connexin 43; cyclin-dependent kinase 5; fatty acid binding protein 7; n-3 polyunsaturated fatty acids