title: Chronic treatment with resveratrol, a natural polyphenol found in grapes, alleviates oxidative stress and apoptotic cell death in ovariectomized female rats subjected to chronic cerebral hypoperfusion.
abstract: OBJECTIVE
  Resveratrol appears to have neuroprotective potential in various animal models of brain disorders including cerebral ischemia and neurodegenerative diseases. Chronic cerebral hypoperfusion is a well-known pathological condition contributing to the neurodegenerative diseases such as vascular dementia. Purpose of the present study is to evaluate the possible therapeutic potential of resveratrol in a model of vascular dementia of ovariectomized female rats. Assessment of the potential was based on the determination of brain oxidative status, caspase-3 level, glial fibrillary acidic protein (GFAP), and neuronal damage on hippocampus and cerebral cortex.
  
  
  METHODS
  For creating the model of chronic cerebral hypoperfusion, ovariectomized female Wistar rats were subjected to the modified two vessel occlusion method, with the right common carotid artery being occluded first and the left one a week later.
  
  
  RESULTS
  At the 15th day following the ligation, neuronal damage was accompanied by the increased immunoreactivities of both GFAP and caspase-3, and significant neurodegeneration was evident in the hippocampus and cortex, all of which were significantly alleviated with resveratrol treatment (10 mg/kg). Biochemical analysis revealed that the resveratrol treatment decreased lipid peroxidation and restored reduced glutathione level as well.
  
  
  CONCLUSIONS
  The collected data of the present study suggest that the administration of resveratrol may provide a remarkable therapeutic benefit for vascular dementia, which is most likely related to the prevention of both apoptotic cell death and oxidative stress. We believe that therapeutic efficacy of resveratrol deserves to be tested for potential clinical application in postmenopausal elderly women suffering from vascular dementia.
authors.affiliation: a Department of Physiology , Bulent Ecevit University Medical School , Kozlu Zonguldak 67600 , Turkey.
authors.forename: Veysel Haktan
authors.initials: VH
authors.lastname: Ozacmak
authors.affiliation: a Department of Physiology , Bulent Ecevit University Medical School , Kozlu Zonguldak 67600 , Turkey.
authors.forename: Hale
authors.initials: H
authors.lastname: Sayan-Ozacmak
authors.affiliation: a Department of Physiology , Bulent Ecevit University Medical School , Kozlu Zonguldak 67600 , Turkey.
authors.forename: Figen
authors.initials: F
authors.lastname: Barut
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D017209:Apoptosis; D015415:Biomarkers / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism / Q000473:pathology; D015140:Dementia, Vascular / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D019587:Dietary Supplements; D004195:Disease Models, Animal; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism / Q000473:pathology; D015227:Lipid Peroxidation; D018696:Neuroprotective Agents / Q000627:therapeutic use; D010052:Ovariectomy / Q000009:adverse effects; D018384:Oxidative Stress; D011897:Random Allocation; D017208:Rats, Wistar; D000077185:Resveratrol; D013267:Stilbenes / Q000627:therapeutic use*
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D005904:Glial Fibrillary Acidic Protein; D018696:Neuroprotective Agents; D013267:Stilbenes; C505410:Casp3 protein, rat; D053148:Caspase 3; D005978:Glutathione; D000077185:Resveratrol
keywords: Caspase-3; Chronic cerebral hypoperfusion; Female rat; Ovariectomy; Oxidative stress; Resveratrol