title: Sesamol Enhances Cell Growth and the Biosynthesis and Accumulation of Docosahexaenoic Acid in the Microalga Crypthecodinium cohnii.
abstract: Sesamol is a strong antioxidant phenolic compound found in sesame seed. It possesses the ability to scavenge intracellular reactive oxygen species (ROS) and to inhibit malic enzyme activity and NADPH supply, resulting possibly in cell proliferation and alteration in the fatty acid composition. In the present study, the effect of sesamol on the growth and accumulation of docosahexaenoic acid (DHA) was investigated in the marine microalga Crypthecodinium cohnii, a prolific producer of DHA. C. cohnii showed a great decrease in the intracellular ROS level with the addition of sesamol. In contrast, the biomass concentration, DHA content (% of total fatty acids), and DHA productivity were significantly increased by 44.20, 11.25, and 20.00%, respectively (P < 0.01). Taken together, this work represents the first report of employing sesamol for enhanced production of DHA by C. cohnii, providing valuable insights into this alga for future biotechnological applications.
authors.affiliation: †School of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510641, China.|§Institute for Food and Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.affiliation: §Institute for Food and Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, China.|#Institute of Marine and Environmental Technology, University of Maryland Center for Environmental Science, Baltimore, Maryland 21202, United States.
authors.forename: Jin
authors.initials: J
authors.lastname: Liu
authors.affiliation: †School of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510641, China.
authors.forename: Peipei
authors.initials: P
authors.lastname: Sun
authors.affiliation: §Institute for Food and Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, China.
authors.forename: Xiaonian
authors.initials: X
authors.lastname: Ma
authors.affiliation: ⊥School of Food Science, Jiangnan University, Wuxi 214122, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: §Institute for Food and Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, China.|ΔSingapore-Peking University Research Centre for a Sustainable Low-Carbon Future, CREATE Tower, Singapore 138602.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
mesh_terms: D052117:Benzodioxoles / Q000494:pharmacology*; D018533:Biomass; D002453:Cell Cycle; D004141:Dinoflagellida / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D004281:Docosahexaenoic Acids / Q000096:biosynthesis*; D058086:Microalgae / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D010636:Phenols / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D052117:Benzodioxoles; D010636:Phenols; D017382:Reactive Oxygen Species; D004281:Docosahexaenoic Acids; C025583:sesamol
keywords: Crypthecodinium cohnii; docosahexaenoic acid; microalgae; reactive oxygen species; sesamol