title: Evolution of porcine reproductive and respiratory syndrome virus GP5 and GP3 genes under swIFN-β immune pressure and interferon regulatory factor-3 activation suppressed by GP5.
abstract: In this study, a highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) strain, PRRSV GD07 was continuously propagated in MARC-145 cell cultures primed with swIFN-β for 50 passages to develop the PRRSV GDβfn strains. And a control strain PRRSV GDfn was passaged without swIFN-β. The sequencing analysis indicated that under swIFN-β immune pressure, molecular variation of PRRSV GP5 was accelerated in gene (NS/S>2.50), and the acceleration of GP3 was not significant (NS/S<2.50). swIFN-β mRNA level induced by Poly(I:C) is lower in cells primed with PRRSV GDβfn than in cells without PRRSV GDfn, although both of them are much less than the control group. Effect of GP5 on IRF3 was analyzed by SDS-PAGE and western-blot. Our results indicated that GP5 protein prevents IRF3 phosphorylation. Therefore, we conclude that swIFN can promote viral mutation in GP5, and, in turn GP5 inhibits IRF3 activation to escape from swIFN-β.
authors.affiliation: South China Agricultural University, Ministry of Agriculture, Key Laboratory of Biotechnology and Bioproducts Development for Animal Epidemic Prevention, 510642, China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Zhixuan
authors.affiliation: South China Agricultural University, Ministry of Agriculture, Key Laboratory of Biotechnology and Bioproducts Development for Animal Epidemic Prevention, 510642, China.
authors.forename: Niu
authors.initials: N
authors.lastname: Xiaoyun
authors.affiliation: South China Agricultural University, Ministry of Agriculture, Key Laboratory of Biotechnology and Bioproducts Development for Animal Epidemic Prevention, 510642, China.
authors.forename: Song
authors.initials: S
authors.lastname: Yabing
authors.affiliation: South China Agricultural University, Ministry of Agriculture, Key Laboratory of Biotechnology and Bioproducts Development for Animal Epidemic Prevention, 510642, China.
authors.forename: Su
authors.initials: S
authors.lastname: Danping
authors.affiliation: South China Agricultural University, Ministry of Agriculture, Key Laboratory of Biotechnology and Bioproducts Development for Animal Epidemic Prevention, 510642, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Fei
authors.affiliation: South China Agricultural University, Ministry of Agriculture, Key Laboratory of Biotechnology and Bioproducts Development for Animal Epidemic Prevention, 510642, China. Electronic address: 1006987655@qq.com.
authors.forename: Chen
authors.initials: C
authors.lastname: Rui-ai
authors.affiliation: South China Agricultural University, Ministry of Agriculture, Key Laboratory of Biotechnology and Bioproducts Development for Animal Epidemic Prevention, 510642, China. Electronic address: dhe@scau.edu.cn.
authors.forename: He
authors.initials: H
authors.lastname: Dongsheng
mesh_terms: D000818:Animals; D015153:Blotting, Western; D018929:Cell Culture Techniques; D002460:Cell Line; D002522:Chlorocebus aethiops; D004268:DNA-Binding Proteins / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D019143:Evolution, Molecular; D050838:Interferon Regulatory Factor-3 / Q000378:metabolism; D016899:Interferon-beta / Q000378:metabolism*; D010766:Phosphorylation; D011070:Poly I-C; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics*; D013552:Swine / Q000821:virology*; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D050838:Interferon Regulatory Factor-3; D014759:Viral Envelope Proteins; C114789:glycoprotein 5, PRRSV; D016899:Interferon-beta; D011070:Poly I-C
keywords: GP5 (glycoprotein-5); IRF3 (interferon regulatory factor-3); Mutations; PRRSV (porcine reproductive and respiratory syndrome virus); Phosphorylation; swIFN-β (swine interferon-β)