title: Oligodendrocyte progenitor cells differentiation of nuclear transferred mouse embryonic stem cells.
abstract: Central nervous system (CNS) injuries cause to variable disorders in people around the world without any decisive treatment. Use of embryonic stem cells (ESCs) would be helpful in repairing of neural system damages. Somatic cell nuclear transfer (SCNT) is a way for implanting ESCs with lowest possible rejection. In the present study, mouse nuclear transfers ESCs (ntESCs) ability in differentiation to oligodendrocyte progenitor cells (OPCs) was investigated by morphological study, RT&mdash;PCR and flow cytometry analysis. Bi&mdash;polar and tri&mdash;polar, OPCs were observed in stem cells cultured in differentiation medium after four weeks. Result of gene expression analysis demonstrated that differentiated stem cells were expressed most important OPCs related gene markers after differentiation period. Moreover, flow cytometry analysis carried out to confirm other results that showed differentiated stem cells significantly expressed NG2 and O4 as critical OPC surface markers. Taken together, it seems that mouse ntESCs showed highly potential for OPC differentiation and could be an appropriate candidate for stem cell therapies.
authors.affiliation: Shahid Beheshti University of Medical Sciences Cellular and Molecular Biology Research Center Tehran Iran m.salehi&commat;sbmu.ac.ir.
authors.forename: M
authors.initials: M
authors.lastname: Salehi
authors.affiliation: Shahid Beheshti University of Medical Sciences Department of Stem Cell Biology, Stem Cell Technology Research Center Tehran Iran.
authors.forename: A
authors.initials: A
authors.lastname: Ardeshirylajimi
authors.affiliation: Shahid Beheshti University of Medical Sciences Department of Stem Cell Biology, Stem Cell Technology Research Center Tehran Iran.
authors.forename: M
authors.initials: M
authors.lastname: Mossahebi-Mohammadi
authors.affiliation: Shahid Beheshti University of Medical Sciences Cellular and Molecular Biology Research Center Tehran Iran.
authors.forename: Z
authors.initials: Z
authors.lastname: Kondori
authors.affiliation: Shahid Beheshti University of Medical Sciences Neurosience Research Center Tehran Iran.
authors.forename: M
authors.initials: M
authors.lastname: Jorjani
mesh_terms: D000818:Animals; D000941:Antigens / Q000096:biosynthesis; D015415:Biomarkers; D002454:Cell Differentiation; D064987:Cell- and Tissue-Based Therapy / Q000379:methods*; D002478:Cells, Cultured; D002490:Central Nervous System / Q000166:cytology / Q000293:injuries*; D005434:Flow Cytometry; D015870:Gene Expression; D020869:Gene Expression Profiling; D051379:Mice; D000066450:Mouse Embryonic Stem Cells / Q000166:cytology*; D009836:Oligodendroglia / Q000166:cytology*; D011509:Proteoglycans / Q000096:biosynthesis; D000099216:Chondroitin Sulfate Proteoglycan 4
chemical_list: D000941:Antigens; D015415:Biomarkers; D011509:Proteoglycans; D000099216:Chondroitin Sulfate Proteoglycan 4