title: The quest for affinity chromatography ligands: are the molecular libraries the right source?
abstract: Affinity chromatography separations of proteins call for highly specific ligands. Antibodies are the most obvious approach; however, except for specific situations, technical and economic reasons are arguments against this choice especially for preparative purposes. With this in mind, the rationale is to select the most appropriate ligands from collections of pre-established molecules. To reach the objective of having a large structural coverage, combinatorial libraries have been proposed. These are classified according to their nature and origin. This review presents and discusses the most common affinity ligand libraries along with the most appropriate screening methods for the identification of the right affinity chromatography selective structure according to the type of library; a side-by-side comparison is also presented.
authors.affiliation: LFB Biotech, Les Ulis, France.
authors.forename: Gérald
authors.initials: G
authors.lastname: Perret
authors.affiliation: LFB Biotech, Les Ulis, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Santambien
authors.affiliation: Bioconsultant, JAM Conseil, Neuilly, France.
authors.forename: Egisto
authors.initials: E
authors.lastname: Boschetti
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods*; D008024:Ligands; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D008024:Ligands; D011506:Proteins
keywords: Affinity chromatography; Aptamers; Ligands; Peptides; Protein purification