title: A new method for measuring osteoclast formation by electrical impedance.
abstract: Osteoclasts are important target cells for osteoporosis treatment. Recently, a real-time cell analysis (RTCA) system was developed to observe cell morphology and adhesion; however, the use of RTCA to study osteoclastogenesis has not been reported. Here, we investigated whether osteoclast formation could be monitored in real-time using RTCA. The cell index determined via electrical impedance using RTCA, and the number of osteoclasts exhibited a significant positive correlation. RTCA was useful for determining the effect of (-)-epigallocatechin-3-gallate on the inhibition of bone resorption. We established a new method of measuring osteoclast formation in real-time using RTCA.
authors.affiliation: Department of Pharmacology, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan; Department of Orthopaedic Surgery, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
authors.forename: Haruka
authors.initials: H
authors.lastname: Emori
authors.affiliation: Department of Healthcare and Regulatory Sciences, Showa University School of Pharmacy, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Iwai
authors.affiliation: Department of Pharmacology, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
authors.forename: Kakei
authors.initials: K
authors.lastname: Ryu
authors.affiliation: Department of Pharmacology, Osaka Dental University, 8-1 Kuzuhahanazono-cho, Hirakata, Osaka 573-1121, Japan. Electronic address: amano@cc.osaka-dent.ac.jp.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Amano
authors.affiliation: Department of Pharmacology, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
authors.forename: Takehiko
authors.initials: T
authors.lastname: Sambe
authors.affiliation: Department of Pharmacology, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kobayashi
authors.affiliation: Department of Pharmacology, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
authors.forename: Tatsunori
authors.initials: T
authors.lastname: Oguchi
authors.affiliation: Department of Pharmacology, Osaka Dental University, 8-1 Kuzuhahanazono-cho, Hirakata, Osaka 573-1121, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Ohura
authors.affiliation: Department of Pharmacology, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
authors.forename: Katsuji
authors.initials: K
authors.lastname: Oguchi
mesh_terms: D001862:Bone Resorption / Q000473:pathology / Q000517:prevention & control; D002392:Catechin / Q000031:analogs & derivatives / Q000494:pharmacology; D002454:Cell Differentiation; D002478:Cells, Cultured; D003199:Computer Systems; D003584:Cytological Techniques / Q000379:methods*; D017097:Electric Impedance; D006801:Humans; D016173:Macrophage Colony-Stimulating Factor; D010010:Osteoclasts / Q000166:cytology*; D053245:RANK Ligand
chemical_list: D053245:RANK Ligand; D016173:Macrophage Colony-Stimulating Factor; D002392:Catechin; C045651:epigallocatechin gallate
keywords: Cell morphology; Osteoclast; Real-time cell analyzing system