title: Dual-signal amplification strategy for ultrasensitive chemiluminescence detection of PDGF-BB in capillary electrophoresis.
abstract: Many efforts have been made toward the achievement of high sensitivity in capillary electrophoresis coupled with chemiluminescence detection (CE-CL). This work describes a novel dual-signal amplification strategy for highly specific and ultrasensitive CL detection of human platelet-derived growth factor-BB (PDGF-BB) using both aptamer and horseradish peroxidase (HRP) modified gold nanoparticles (HRP-AuNPs-aptamer) as nanoprobes in CE. Both AuNPs and HRP in the nanoprobes could amplify the CL signals in the luminol-H2 O2 CL system, owing to the excellent catalytic behavior of AuNPs and HRP in the CL system. Meanwhile, the high affinity of aptamer modified on the AuNPs allows detection with high specificity. As proof-of-concept, the proposed method was employed to quantify the concentration of PDGF-BB from 0.50 to 250 fm with a detection limit of 0.21 fm. The applicability of the assay was further demonstrated in the analysis of PDGF-BB in human serum samples with acceptable accuracy and reliability. The result of this study exhibits distinct advantages, such as high sensitivity, good specificity, simplicity, and very small sample consumption. The good performances of the proposed strategy provide a powerful avenue for ultrasensitive detection of rare proteins in biological sample, showing great promise in biochemical analysis.
authors.affiliation: College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang, 464000, China.
authors.forename: Jun-Tao
authors.initials: JT
authors.lastname: Cao
authors.affiliation: College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang, 464000, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Xinyang Central Hospital, Xinyang, 464000, China.
authors.forename: Shu-Wei
authors.initials: SW
authors.lastname: Ren
authors.affiliation: Xinyang Central Hospital, Xinyang, 464000, China.
authors.forename: Yong-Hong
authors.initials: YH
authors.lastname: Chen
authors.affiliation: College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang, 464000, China.
authors.forename: Yan-Ming
authors.initials: YM
authors.lastname: Liu
mesh_terms: D000077214:Becaplermin; D003928:Diabetic Nephropathies / Q000097:blood; D019075:Electrophoresis, Capillary / Q000379:methods*; D006046:Gold / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry; D020574:Proto-Oncogene Proteins c-sis / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D015203:Reproducibility of Results; D012815:Signal Processing, Computer-Assisted
chemical_list: D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D008165:Luminol; D006046:Gold; D006735:Horseradish Peroxidase
keywords: HRP-AuNPs-aptamer; PDGF-BB; capillary electrophoresis; chemiluminescence; dual signal amplification