title: MicroRNA expression profile of surgical removed mandibular bone tissues from patients with mandibular prognathism.
abstract: BACKGROUND
  Mandibular prognathism (MP) or skeletal class III malocclusion with a prognathic mandible is one of the most severe facial deformities. Recent work has revealed certain circulating microRNAs (miRNAs) are associated with MP, we conducted this study to characterize the miRNAs expression profile in surgically removed mandibular bone tissue in patients with MP and explored the role of miRNA regulation in the pathogenesis of MP.
  
  
  METHODS
  Affymetrix GeneChip miRNA 3.0 Array was used to examine the miRNA expression in mandibular bone tissues from MP patients and control subjects. A variety of bioinformatic approaches were used to predict the target genes of the miRNAs, find the potential functions and pathways of the target genes, analyze their intersection with differentially expressed mRNAs, and establish miRNA-gene network.
  
  
  RESULTS
  Eleven upregulated and 11 downregulated miRNAs with a fold change ≥ 2 and a P value <0.05 were identified in bone specimens of MP patients. A total of 3569 genes were predicted as targets of hsa-miR-10a-5p, hsa-miR-150-5p, hsa-miR-192-5p, hsa-miR-194-5p, hsa-miR-197-3p, hsa-miR-30 d-5p, hsa-miR-342-5p and hsa-miR-629-5p, hsa-miR-1202, and hsa-miR-638. The target genes were predicted to be involved in biological functions and signaling pathways related to osteogenesis. Hsa-miR-30 d-5p was the key node of miRNA-gene network.
  
  
  CONCLUSIONS
  Our results indicated a possible association between the differentially expressed miRNAs and MP pathogenesis, and the precise mechanisms are needed to be further validated.
authors.affiliation: Department of Orthodontics, School of Stomatology, China Medical University, Shenyang, People's Republic of China; Department of Orthodontics, Liaoning Institute of Dental Research, Shenyang, People's Republic of China. Electronic address: tianyulou@hotmail.com.
authors.forename: Yulou
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Experiment and Technology Center, China Medical University, Shenyang, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Stomatology, China Medical University, Shenyang, People's Republic of China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Bai
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Stomatology, China Medical University, Shenyang, People's Republic of China.
authors.forename: Xuexin
authors.initials: X
authors.lastname: Tan
authors.affiliation: Department of Orthodontics, School of Stomatology, China Medical University, Shenyang, People's Republic of China.
authors.forename: Yuming
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Department of Orthodontics, School of Stomatology, China Medical University, Shenyang, People's Republic of China.
authors.forename: Ke
authors.initials: K
authors.lastname: Qin
authors.affiliation: Department of Orthodontics, School of Stomatology, China Medical University, Shenyang, People's Republic of China.
authors.forename: Zhenjin
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Department of Orthodontics, School of Stomatology, China Medical University, Shenyang, People's Republic of China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000328:Adult; D053263:Gene Regulatory Networks; D006801:Humans; D008334:Mandible / Q000378:metabolism*; D035683:MicroRNAs / Q000032:analysis*; D020411:Oligonucleotide Array Sequence Analysis; D025341:Principal Component Analysis; D011378:Prognathism / Q000209:etiology / Q000235:genetics* / Q000601:surgery; D059467:Transcriptome
chemical_list: D035683:MicroRNAs
keywords: Gene ontology analysis; Mandibular prognathism; MicroRNA-gene network; MicroRNAs; Microarray analysis; Pathway analysis