title: Antiviral activity ofBifidobacterium adolescentisSPM1605 against Coxsackievirus B3.
abstract: Bifidobacteria are considered one of the most beneficial probiotics and have been widely studied for their effects against specific pathogens. The present study investigated the antiviral activity of probiotics isolated from Koreans against Coxsackievirus B3 (CVB3). The effect of probiotic isolates against CVB3 was measured by the plaque assay and cellular toxicity of bifidobacteria in HeLa cells was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Among 13 probiotic isolates, 3Bifidobacterium adolescentis, 2Bifidobacterium longumand 1Bifidobacterium pseudocatenulatumhad an antiviral effect against CVB3, while the others did not show such effect.B. adolescentisSPM1605 showed the greatest inhibitory properties against CVB3. When the threshold cycle (CT) values for the treatedB. adolescentisSPM1605 samples were compared to the results for the non-treated samples, it was shown that the amplified viral sequences from the CVB3 had their copy number lowered byB. adolescentisSPM1605. Moreover, the gene expression in infected HeLa cells was also inhibited by 50%. The results suggest thatB. adolescentisSPM1605 suppresses CVB3 and could be used as an alternative therapy against infectious diseases caused by coxsackieviruses.
authors.affiliation: College of Pharmacy, Sahmyook University , Seoul , Republic of Korea.
authors.forename: Min Ji
authors.initials: MJ
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Sahmyook University , Seoul , Republic of Korea.
authors.forename: Do Kyung
authors.initials: DK
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Sahmyook University , Seoul , Republic of Korea.
authors.forename: Jae Eun
authors.initials: JE
authors.lastname: Park
authors.affiliation: College of Pharmacy, Sahmyook University , Seoul , Republic of Korea.
authors.forename: Il Ho
authors.initials: IH
authors.lastname: Park
authors.affiliation: R&D Center, Cellbiotech, Co. Ltd. , Gimpo , Republic of Korea.
authors.forename: Jae Gu
authors.initials: JG
authors.lastname: Seo
authors.affiliation: College of Pharmacy, Sahmyook University , Seoul , Republic of Korea.
authors.forename: Nam Joo
authors.initials: NJ
authors.lastname: Ha
keywords: Bifidobacterium adolescentis; Coxsackievirus; HeLa cells; plaque reduction assay; real-time qPCR