title: Assessment of the effect of prolonged forced swimming on CD-1 mice sperm morphology with and without antioxidant supplementation.
abstract: As physical exercise has been shown to negatively affect sperm morphology, this study was undertaken to assess the effect of a 3-min forced swimming protocol during 50 days, with and without administration of antioxidants [N-acetylcysteine (NAC) and trans-resveratrol], on sperm morphology in CD-1 mice. Forty-four 13-week-old CD-1 mice were randomly allocated to four different groups: mice not submitted to exercise, control group (CG), mice submitted to swimming without administration of antioxidants (EX), mice submitted to swimming that received trans-resveratrol supplementation [exercise group (EX)+Resv] and mice submitted to swimming exercise that received NAC supplementation (EX+NAC). The EX showed 30.5% of spermatozoa with normal morphology, showing significant differences with regard to the CG, which showed 58.5%. The groups receiving antioxidant supplements showed significantly higher percentages of spermatozoa with normal morphology in comparison with the EX group (EX+Resv: 64.1%, EX+NAC: 48.2%). The imposed model of forced swimming caused alterations in sperm morphology. The antioxidants employed seem to be suitable antioxidants for avoiding exercise-associated sperm morphology anomalies in prolonged forced swimming exercise. Trans-resveratrol has proven to be more efficient for this purpose.
authors.affiliation: Animal Surgery and Medicine Department, School of Veterinary Medicine, University of Cordoba, Cordoba, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Rodriguez
authors.affiliation: Animal Surgery and Medicine Department, School of Veterinary Medicine, University of Cordoba, Cordoba, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Diaz
authors.affiliation: Morphological Sciences Department, School of Medicine, University of Cordoba, Cordoba, Spain.
authors.forename: D
authors.initials: D
authors.lastname: Vaamonde
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D048430:Cell Shape / Q000187:drug effects / Q000502:physiology*; D008297:Male; D051379:Mice; D010805:Physical Conditioning, Animal / Q000502:physiology; D000077185:Resveratrol; D013094:Spermatozoa / Q000166:cytology* / Q000187:drug effects; D013267:Stilbenes / Q000494:pharmacology*; D013550:Swimming / Q000502:physiology*
chemical_list: D000975:Antioxidants; D013267:Stilbenes; D000077185:Resveratrol; D000111:Acetylcysteine
keywords: N-acetylcysteine; sperm morphology; swimming exercise; trans-resveratrol