title: The effect of Curcuma longa extracted (curcumin) on the quality of cryopreserved boar semen.
abstract: The aim of this study was to determine the optimal concentration of curcumin needed for cryopreservation of boar semen. Semen samples (n = 9) were collected from nine Duroc boars which having proven fertility were used for routine artificial insemination. Semen samples were collected and divided into six groups (groups A-F) according to various concentrations of curcumin in freezing extender (i.e. 0, 0.125, 0.25, 0.50, 0.75 and 1.0 mmol/L, respectively). The semen was frozen by traditional liquid nitrogen vapor method and stored at -196°C in the liquid nitrogen tank. After storage, frozen semen samples were thawed at 50°C for 12 s and evaluated for progressive motility, viability and acrosome integrity. The present results indicated that the addition of curcumin at 0.25 (group C) or 0.50 mmol/L curcumin (group D) yielded the higher percentage of progressive motility (33.3 and 36.1%, respectively) (P < 0.001). A significantly higher percentage of acrosome integrity was found in groups B (29.7%), C (31.1%) and D (30.2%) than in the other groups (P < 0.01). However, there was no significant difference in percentage of viability among groups. In conclusion, addition to the freezing extender of curcumin during cryopreservation at a concentration of 0.25 or 0.50 mmol/L is the optimal concentration of curcumin for improving the quality (i.e. increased progressive motility and acrosome integrity) of cryopreserved boar semen.
authors.affiliation: Semen Laboratory, Department of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, Phuttamonthon, Nakhon-Pathom, Thailand.
authors.forename: Panida
authors.initials: P
authors.lastname: Chanapiwat
authors.affiliation: Semen Laboratory, Department of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, Phuttamonthon, Nakhon-Pathom, Thailand.
authors.forename: Kampon
authors.initials: K
authors.lastname: Kaeoket
mesh_terms: D020101:Acrosome Reaction / Q000187:drug effects; D000818:Animals; D003474:Curcumin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D008297:Male; D055101:Semen Analysis; D012662:Semen Preservation; D013081:Sperm Motility / Q000187:drug effects; D013552:Swine
chemical_list: D003474:Curcumin
keywords: antioxidant; boar semen; cryopreservation; curcumin