title: Scolicidal activity of biosynthesized silver nanoparticles against Echinococcus granulosus protoscolices.
abstract: Cystic hydatid disease (CHD), a helminth infection with various clinical complications caused by the larval stage of the dog tapeworm Echinococcus granulosus is considered as a public health problem in different regions of the world. To date, scolicidal agents have been broadly applied for inactivation of the fertile cysts and these scolicidal agents have several side effects on patients. Therefore, the objective of this study was to evaluate the scolicidal efficacies of synthesized silver nanoparticles (AgNPs) derived from the aqueous aerial extract of Penicillium aculeatum against protoscolices of CHD in-vitro. Protoscolices were aseptically aspirated from infected livers of sheep with CHD. Various concentrations (0.025, 0.05, 0.1 and 0.15 mg/mL) of green synthesis of Ag-NPs and different exposure times (10, 30, 60 and 120 min) were used against protoscolices of CHD. Viability of protoscolices was confirmed by 0.1% eosin staining. The findings showed that the Ag-NPs at all concentrations have high scolicidal effects. The concentrations 0.1 and 0.15 mg/mL after 120 min of exposure times showed 83% and 90% mortality rate, respectively. The least scolicidal activity of biosynthesized Ag-NPs was 40% (0.025 mg/mL and 10 min). The current investigation indicated that applying biogenic Ag-NPs may be considered as a potential scolicidal agent for CHD surgery due to being economical, safer and non-toxic compared to the used chemical materials. However, further studies are required to evaluate the efficacy of Ag-NPs in vivo.
authors.affiliation: Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Mohammad Taghi
authors.initials: MT
authors.lastname: Rahimi
authors.affiliation: Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: ehsanahmadpour@gmail.com.
authors.forename: Ehsan
authors.initials: E
authors.lastname: Ahmadpour
authors.affiliation: Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical, Tehran, Iran.
authors.forename: Bahman
authors.initials: B
authors.lastname: Rahimi Esboei
authors.affiliation: Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Adel
authors.initials: A
authors.lastname: Spotin
authors.affiliation: Department of Medical Parasitology and Mycology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
authors.forename: Mohammad Hasan
authors.initials: MH
authors.lastname: Kohansal Koshki
authors.affiliation: Department of Epidemiology and Biostatistics, School of Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Ahad
authors.initials: A
authors.lastname: Alizadeh
authors.affiliation: Mazandaran University of Medical Sciences, School of Pharmacy, Sari, Iran.
authors.forename: Soheila
authors.initials: S
authors.lastname: Honary
authors.affiliation: Mazandaran University of Medical Sciences, School of Pharmacy, Sari, Iran.
authors.forename: Hamed
authors.initials: H
authors.lastname: Barabadi
authors.affiliation: Mazandaran University of Medical Sciences, School of Pharmacy, Sari, Iran.
authors.forename: Milad
authors.initials: M
authors.lastname: Ali Mohammadi
mesh_terms: D000818:Animals; D004443:Echinococcosis / Q000188:drug therapy* / Q000469:parasitology; D048209:Echinococcus granulosus / Q000187:drug effects*; D006801:Humans; D053758:Nanoparticles; D012834:Silver / Q000494:pharmacology*
chemical_list: D012834:Silver
keywords: Cystic hydatid disease; Echinococcus granulosus; Green silver nanoparticles; Scolicidal