title: Cardiac and renal nitrosative-oxidative stress after acute poisoning by a nerve agent Tabun.
abstract: We hypothesized that Tabun poisoning, as well as other organophosphorous treatment, cause specific organs' oxidative changes that have not previously been substantiated investigated. In this regard, a marker for nitrosative-oxidative stress in the main haemodynamic organs (heart and kidney) could reveal the existence of such changes. In this study, for the first time we studied the nitrosative/oxidative stress in heart and kidney after acute Tabun (Ethyl N,N- Dimethylphosphoramidocyanidate) poisoning measuring by immunohistochemistry the expression of 3-nitrotyrosine--a marker for nitrosative-oxidative stress. We investigated nitrotyrozine expression in three different groups of animals (with at least 3 animals in each group): the first group was treated with 0.5 LD50 Tabun and organs were collected after 24 h; the second group received vehicle for the same period; in the third group a highly specific re-activator was applied immediately after Tabun application. Heart and kidney were collected after 24 h. The levels of nitrotyrozine production significantly increased (more than 3 times) in cardiomyocytes after Tabun. The application of re-activator slightly reduced these levels not reaching the basal heart levels. Nitrotyrozine expression in kidney increased more than 2 times after Tabun and application of re-activator did not change it significantly. In conclusion, our study evidently demonstrated that Tabun trigger oxidative-nitrosative stress in heart and kidney and these cellular effects should be protected by an additional anti-oxidant therapy, since acetylcholinesterase re-activator is not efficient in this manner.
authors.affiliation: a Disaster Medicine and Toxicology Deparment , Military Medical Academy , Sofia , Bulgaria.
authors.forename: Dimo
authors.initials: D
authors.lastname: Dimov
authors.forename: Radka
authors.initials: R
authors.lastname: Hadjiolova
authors.forename: Kamen
authors.initials: K
authors.lastname: Kanev
authors.forename: Radka
authors.initials: R
authors.lastname: Tomova
authors.forename: Anna
authors.initials: A
authors.lastname: Michova
authors.forename: Todor
authors.initials: T
authors.lastname: Todorov
authors.forename: Rumen
authors.initials: R
authors.lastname: Murdjev
authors.forename: Temenujka
authors.initials: T
authors.lastname: Boneva
authors.forename: Ivanka
authors.initials: I
authors.lastname: Dimova
mesh_terms: D000818:Animals; D006321:Heart / Q000187:drug effects*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D000067397:Nerve Agents / Q000633:toxicity; D010755:Organophosphates / Q000633:toxicity*; D010084:Oxidation-Reduction / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D014443:Tyrosine / Q000031:analogs & derivatives
chemical_list: D000067397:Nerve Agents; D010755:Organophosphates; C002744:3-nitrotyrosine; D014443:Tyrosine; C009374:tabun
keywords: 3-nitrotyrozine; Organophosphorous poisoning; cardiac and renal damage; nitrosative-oxidative stress