title: Differentially expressed genes in adipocytokine signaling pathway of adipose tissue in pregnancy.
abstract: OBJECTIVE
  To profile the differential gene expression of the KEGG Adipocytokine Signaling pathway in omental compared to subcutaneous tissue in normal pregnancy.
  
  
  METHODS
  Subjects included 14 nonobese, normal glucose tolerant, healthy pregnant women. Matched omental and subcutaneous tissue were obtained at elective cesarean delivery. Gene expression was evaluated using microarray and validated by RT-PCR. Differential gene expression was defined as ≥1.5 fold increase at p < 0.05.
  
  
  RESULTS
  Six genes were significantly downregulated with two upregulated genes in omental tissue. Downregulation of Adiponectin and Insulin Receptor substrate, key genes mediating insulin sensitivity, were observed with borderline upregulation of GLUT-1. There were downregulations of CD36 and acyl-CoA Synthetase Long-chain Family Member 1which are genes involved in fatty acid uptake and activation. There was a novel expression of Carnitine palmitoyltransferase 1C.
  
  
  CONCLUSIONS
  Differential gene expression of Adipocytokine Signaling Pathway in omental relative to subcutaneous adipose tissue in normal pregnancy suggests a pattern of insulin resistance, hyperlipidemia, and inflammation.
authors.affiliation: Department of Obstetrics and Gynecology, Cedars-Sinai Medical Center, Los Angeles, USA ; Department of Medical Education, David Geffen School of Medicine at UCLA, Los Angeles, USA.
authors.forename: Dotun
authors.initials: D
authors.lastname: Ogunyemi
authors.affiliation: Department of Medical Genetics, Cedars-Sinai Medical Center, Los Angeles, USA.
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Obstetrics and Gynecology, Cedars-Sinai Medical Center, Los Angeles, USA.
authors.forename: Arnold M
authors.initials: AM
authors.lastname: Mahesan
authors.affiliation: Department of Obstetrics and Gynecology, Cedars-Sinai Medical Center, Los Angeles, USA.
authors.forename: Steve
authors.initials: S
authors.lastname: Rad
authors.affiliation: Department of Medical Genetics, Cedars-Sinai Medical Center, Los Angeles, USA.
authors.forename: Eric
authors.initials: E
authors.lastname: Kim
authors.affiliation: Department of Obstetrics and Gynecology, Cedars-Sinai Medical Center, Los Angeles, USA.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Yano
authors.affiliation: Department of Obstetrics and Gynecology, Cedars-Sinai Medical Center, Los Angeles, USA.
authors.forename: Carolyn
authors.initials: C
authors.lastname: Alexander
authors.affiliation: Department of Medical Education, David Geffen School of Medicine at UCLA, Los Angeles, USA.
authors.forename: Jerome I
authors.initials: JI
authors.lastname: Rotter
authors.affiliation: Department of Endocrinology, Cedars-Sinai Medical Center, Los Angeles, USA.
authors.forename: Y-D Ida
authors.initials: YD
authors.lastname: Chen
keywords: Adipocytokine Pathway; Adipose Tissue; Insulin Resistance; Pregnancy