title: Protective effect of Drosophila glutathione transferase omega 1 against hydrogen peroxide-induced neuronal toxicity.
abstract: Glutathione transferase omega (GSTO) belongs to a recently identified family of glutathione transferase (GST) and presents several known functions. In Drosophila, despite the high sequence identity among the four GstO isoforms, they present different physiological functions. Herein, we showed that GstO1, which is one of the Drosophila GstOs, is highly expressed in adult heads. We determined the three-dimensional structure of GstO1, by homology modeling. Furthermore, we show that GstO1 loss-of-function mutant flies display reduced survival than the control flies when subjected to H2O2 treatment. Interestingly, the neuronal-specific expression of GstO1 in a GstO1 loss-of-function mutant background rescued H2O2-induced toxicity. We further showed that GstO1 inhibits H2O2-mediated activation of the mitogen-activated protein kinase (MAPK) pathway. Collectively, our findings provide valuable new insights into the tissue-specific protective mechanisms of Drosophila GstOs during oxidative stress.
authors.affiliation: Department of Medical Biotechnology, Soonchunhyang University, Asan 336-745, Republic of Korea.
authors.forename: So Yeon
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Department of Medical Biotechnology, Soonchunhyang University, Asan 336-745, Republic of Korea.
authors.forename: In-Ae
authors.initials: IA
authors.lastname: Lim
authors.affiliation: Department of Medical Biotechnology, Soonchunhyang University, Asan 336-745, Republic of Korea.
authors.forename: Ga-Un
authors.initials: GU
authors.lastname: Kang
authors.affiliation: Department of Medical Biotechnology, Soonchunhyang University, Asan 336-745, Republic of Korea.
authors.forename: Sun-Joo
authors.initials: SJ
authors.lastname: Cha
authors.affiliation: Soochunhyang Institute of Medi-bio Science, Soonchunhyang University, Asan 336-745, Republic of Korea.
authors.forename: Volodya
authors.initials: V
authors.lastname: Altanbyek
authors.affiliation: Convergence Brain Research Department, Korea Brain Research Institute, Daegu 700-010, Republic of Korea.
authors.forename: Hyung-Jun
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Korea Basic Science Institute Gwangju Center, Gwangju 500-757, Republic of Korea.
authors.forename: Seongsoo
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Medical Biotechnology, Soonchunhyang University, Asan 336-745, Republic of Korea. Electronic address: kiyoung2@sch.ac.kr.
authors.forename: Kiyoung
authors.initials: K
authors.lastname: Kim
authors.affiliation: Department of Medical Biotechnology, Soonchunhyang University, Asan 336-745, Republic of Korea.
authors.forename: Jeongbin
authors.initials: J
authors.lastname: Yim
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D001921:Brain / Q000166:cytology / Q000201:enzymology; D029721:Drosophila Proteins / Q000502:physiology*; D004331:Drosophila melanogaster / Q000201:enzymology*; D005982:Glutathione Transferase / Q000502:physiology*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D020935:MAP Kinase Signaling System; D008297:Male; D008969:Molecular Sequence Data; D009474:Neurons / Q000201:enzymology; D009928:Organ Specificity; D016877:Oxidants / Q000494:pharmacology*; D018384:Oxidative Stress
chemical_list: D029721:Drosophila Proteins; D016877:Oxidants; D006861:Hydrogen Peroxide; D005982:Glutathione Transferase; C000600412:GstO1 protein, Drosophila
keywords: Drosophila; Erk; Glutathione transferase omega; Hydrogen peroxide; MAPK pathway; Neuronal cell