title: Purification and characterization of a glucan from Bacillus Calmette Guerin and the antitumor activity of its sulfated derivative.
abstract: A water-soluble glucan, BCG-PASW, with a molecular weight of 2.10×10(4)Da, was separated from polysaccharide nucleic acid fraction of Bacillus Calmette Guerin (BCG-PSN) using DEAE-52 cellulose and Sephadex G-200 chromatography. Based on gas chromatography-mass spectrometry (GC-MS), fourier transform infrared (FT-IR) spectra, 1D and 2D nuclear magnetic resonance (NMR) spectroscopy techniques (COSY, HSQC and HMBC), BCG-PASW was found to be an α-d-glucan composed of α-d-(1→4)-linked glucopyranosyl residues, with branches at O-6 consisting of non-reducing terminal α-d-Glcp approximately every eight residues. In vitro antitumor activity by MTS method, its sulfated derivative with a substitution degree of 0.59, could inhibite C666-1 nasopharyngeal carcinoma cells growth significantly. The results indicated that the sulfate content play a decisive role in the bioactivities of the polysaccharides.
authors.affiliation: Department of Pharmacy, Xiangya Hospital, Changsha 410008, China; School of Pharmacy, Central South University, Changsha 410013, China.
authors.forename: Xiongbo
authors.initials: X
authors.lastname: Xu
authors.affiliation: Jiuzhitang Co., Ltd, Changsha 410021, China.
authors.forename: Zixin
authors.initials: Z
authors.lastname: Gu
authors.affiliation: Department of Pharmacy, Xiangya Hospital, Changsha 410008, China; School of Pharmacy, Central South University, Changsha 410013, China. Electronic address: liushao999@hotmail.com.
authors.forename: Shao
authors.initials: S
authors.lastname: Liu
authors.affiliation: Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
authors.forename: Na
authors.initials: N
authors.lastname: Gao
authors.affiliation: Tumor Institute of Central South University, Changsha 410078, China.
authors.forename: Xiaozhen
authors.initials: X
authors.lastname: He
authors.affiliation: Jiuzhitang Co., Ltd, Changsha 410021, China.
authors.forename: Xiu
authors.initials: X
authors.lastname: Xin
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005936:Glucans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D006868:Hydrolysis; D008745:Methylation; D009005:Monosaccharides / Q000032:analysis; D009163:Mycobacterium bovis; D011725:Pyridines / Q000737:chemistry; D013451:Sulfonic Acids / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D005936:Glucans; D009005:Monosaccharides; D011725:Pyridines; D013451:Sulfonic Acids; C013880:chlorosulfonic acid; C023666:pyridine
keywords: Antitumor; BCG-PSN; Characterization; Chlorosulfonic acid (CID: 24638); Methoxyamine (CID: 4113); N,O-Bis(trime.silyl)trifluoroacetamide (CID: 5366669); Purification; Sulfated derivative; d-Arabinose (CID: 66308); d-Glucopyranose (CID: 5793); d-Mannose (CID: 18950); d-Ribose (CID: 5311110)