title: Arbuscular mycorrhizal fungal communities and Rhizophagus irregularis populations shift in response to short-term ploughing and fertilisation in a buffer strip.
abstract: Short-term effects of soil physical disturbance by ploughing and nitrogen and phosphate fertilisation on arbuscular mycorrhizal fungal (AMF) communities and on intraspecific populations of Rhizophagus irregularis in a buffer strip surrounded by arable fields were studied. Pre-grown Plantago lanceolata plantlets were transplanted into fertilised and/or ploughed experimental plots. After 3 months, the glomeromycotan communities in the roots of these trap plants were analysed using 454 pyrosequencing of a fragment of the RNA polymerase II gene (RPB1). Intraspecific populations of R. irregularis were studied by restriction fragment length polymorphism (RFLP) analysis of the mitochondrial large ribosomal subunit (mtLSU) gene. Soil disturbance significantly increased the diversity of species-level molecular taxa (MTs) and altered community structure, whilst fertilisation alone had no significant effect, unless coupled with ploughing. At the population level, the expected shift from genotypes of R. irregularis typically found in grasslands to those usually found in arable sites was only partially observed. In conclusion, in the short-term, physical soil disturbance, as well as nitrogen fertilisation when coupled with physical soil disturbance, affected AMF community and to a smaller extent population composition.
authors.affiliation: INRA, UMR 1347 Agroécologie, 17 Rue Sully, BP 86510, 21065, Dijon Cedex, France.
authors.forename: M
authors.initials: M
authors.lastname: Peyret-Guzzon
authors.affiliation: INRA, UMR 1347 Agroécologie, 17 Rue Sully, BP 86510, 21065, Dijon Cedex, France.
authors.forename: H
authors.initials: H
authors.lastname: Stockinger
authors.affiliation: INRA, UMR 1347 Agroécologie, 17 Rue Sully, BP 86510, 21065, Dijon Cedex, France.
authors.forename: M-L
authors.initials: ML
authors.lastname: Bouffaud
authors.affiliation: Domaine Expérimental d'Epoisses, UE 0115 INRA, Dijon, 21110, Bretenières, France.
authors.forename: P
authors.initials: P
authors.lastname: Farcy
authors.affiliation: Université de Bourgogne, UMR 1347 Agroécologie, 17 Rue Sully, BP 86510, 21065, Dijon Cedex, France.
authors.forename: D
authors.initials: D
authors.lastname: Wipf
authors.affiliation: Université de Bourgogne, UMR 1347 Agroécologie, 17 Rue Sully, BP 86510, 21065, Dijon Cedex, France. dirk.redecker@dijon.inra.fr.
authors.forename: D
authors.initials: D
authors.lastname: Redecker
mesh_terms: D000383:Agriculture; D004271:DNA, Fungal / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005308:Fertilizers; D014644:Genetic Variation; D055137:Glomeromycota / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D065948:Grassland; D000069396:Mitochondrial Ribosomes / Q000382:microbiology; D038821:Mycorrhizae / Q000378:metabolism / Q000502:physiology*; D018517:Plant Roots / Q000382:microbiology; D012319:RNA Polymerase II / Q000235:genetics; D054658:Ribosome Subunits, Large / Q000235:genetics; D017422:Sequence Analysis, DNA; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology; D013559:Symbiosis
chemical_list: D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D005308:Fertilizers; D012987:Soil; D012319:RNA Polymerase II
keywords: Fertilisation; Glomeromycota; Next-generation sequencing; RPB1; Tillage; mtLSU