title: Is mouse embryonic stem cell technology obsolete?
abstract: Injection of recombinant Cas9 protein and synthetic guide RNAs into mouse zygotes has been shown to facilitate gene disruption and knock-ins using the CRISPR system. These technologies may soon displace genetic modification using embryonic stem cells.
authors.affiliation: Stem Cell Engineering, Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK. skarnes@sanger.ac.uk.
authors.forename: William C
authors.initials: WC
authors.lastname: Skarnes
mesh_terms: D000818:Animals; D064113:CRISPR-Cas Systems / Q000235:genetics*; D003001:Cloning, Molecular; D005260:Female; D055879:Gene Knock-In Techniques; D006801:Humans; D008297:Male