title: The first complete mitochondrial genome of stag beetle from China, Prosopocoilus gracilis (Coleoptera, Lucanidae).
abstract: The complete mitochondrial genome (mitogenome) of Prosopocoilus gracilis (Coleoptera: Lucanidae) that is endemic to Southern China is determined. The circular genome is 736 bp in length and comprises 13 protein-coding genes, 22 tRNA, 2 rRNA genes and a control region. Gene order is identical to that of the putative ancestral arrangement of insects. The nucleotide composition of heavy strand is A (36.6%), C (22.6%), T (29.5%) and G (11.3%). All protein-coding genes start with a typical ATN codon except for the gene COI that uses AAC as the start codon. tRNA-Ser (AGN) uses the anticodon UCU instead of the commonly used GCU. Both maximum likelihood and Bayesian analyses support the monophyly of Lucanidae and the sister relationship of Nigidionus and the remaining sampled genera. Two species of Prosopocoilus were not recovered as a monophyletic group.
authors.affiliation: a Department of Ecology , School of Resources & Engineering, Anhui University , Hefei , P.R. China.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Wu
authors.affiliation: a Department of Ecology , School of Resources & Engineering, Anhui University , Hefei , P.R. China.
authors.forename: Yu-Yan
authors.initials: YY
authors.lastname: Cao
authors.affiliation: a Department of Ecology , School of Resources & Engineering, Anhui University , Hefei , P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Fang
authors.affiliation: a Department of Ecology , School of Resources & Engineering, Anhui University , Hefei , P.R. China.
authors.forename: Xia
authors.initials: X
authors.lastname: Wan
mesh_terms: D000818:Animals; D001499:Bayes Theorem; D002681:China; D018387:Codon, Initiator / Q000235:genetics; D001517:Coleoptera / Q000145:classification / Q000235:genetics*; D049750:Genome, Insect / Q000235:genetics; D054629:Genome, Mitochondrial / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D018387:Codon, Initiator; D012335:RNA, Ribosomal; D012343:RNA, Transfer
keywords: Long PCR; Prosopocoilus gracilis; mitogenome; next-generation sequencing