title: Cryopreservation effect on proliferation and differentiation potential of cultured chorion cells.
abstract: BACKGROUND
  Fetoplacental tissues including the early chorion contain stem cells with various morphological and functional characteristics. Cultured chorionic cells may be used in perspective therapies of different pathologies.
  
  
  OBJECTIVE
  To investigate the effect of cryopreservation on proliferation and differentiation potential of chorion cell culture (ChCC).
  
  
  METHODS
  Five freezing programs for ChCC were compared: Program 1, cooling from 25 degrees C down to -30 degrees C at 0.5 degrees C/min; Program 2, cooling from 25 degrees C down to -30 degrees C at 1 degrees C/min; Program 3, cooling from 25 degrees C down to -10 degrees C at 1 degrees C/min with further cooling down to - 80 degrees C at 10 degrees C/min; Program 4, cooling from 25 degrees C down to -5 degrees C at 1 degrees C/min with further cooling down to -80 degrees C at 10 degrees C/min; Program 5, cooling from 25 degrees C down to -6 degrees C at 1 degrees C/min with further crystal seeding by adding the surplus nitrogen into the chamber, and cooling down to -80 degrees C at 10 degrees C/min. Viability, adhesion, proliferation and directed differentiation were examined.
  
  
  RESULTS
  Freezing program 5 achieved the best result, with the highest viability, adhesion, proliferation and directed differentiation.
  
  
  CONCLUSIONS
  The data may help establishing better cryopreservation protocols for perspective chorionic cell lines and their further application in biotechnology.
authors.forename: N A
authors.initials: NA
authors.lastname: Volkova
authors.forename: A N
authors.initials: AN
authors.lastname: Goltsev
mesh_terms: D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D002823:Chorion / Q000166:cytology*; D015925:Cryopreservation / Q000379:methods*; D005615:Freezing; D006801:Humans