title: Chlamydomonas reinhardtii cells adjust the metabolism to maintain viability in response to atrazine stress.
abstract: Chlamydomonas reinhardtii cells were exposed to a sublethal concentration of the widespread herbicide atrazine for 3 and 24h. Physiological parameters related to cellular energy status, such as cellular activity and mitochondrial and cytoplasmic membrane potentials, monitored by flow cytometry, were altered in microalgal cells exposed to 0.25μM of atrazine. Transcriptomic analyses, carried out by RNA-Seq technique, displayed 12 differentially expressed genes between control cultures and atrazine-exposed cultures at both tested times. Many cellular processes were affected, but the most significant changes were observed in genes implicated in amino acid catabolism and respiratory cellular process. Obtained results suggest that photosynthesis inhibition by atrazine leads cells to get energy through a heterotrophic metabolism to maintain their viability.
authors.affiliation: Laboratorio de Microbiología, Facultad de Ciencias, Universidad de A Coruña, Campus de A Zapateira s/n, 15071 A Coruña, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Esperanza
authors.affiliation: Laboratorio de Microbiología, Facultad de Ciencias, Universidad de A Coruña, Campus de A Zapateira s/n, 15071 A Coruña, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Seoane
authors.affiliation: Laboratorio de Microbiología, Facultad de Ciencias, Universidad de A Coruña, Campus de A Zapateira s/n, 15071 A Coruña, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Rioboo
authors.affiliation: Laboratorio de Microbiología, Facultad de Ciencias, Universidad de A Coruña, Campus de A Zapateira s/n, 15071 A Coruña, Spain.
authors.forename: Concepción
authors.initials: C
authors.lastname: Herrero
authors.affiliation: Laboratorio de Microbiología, Facultad de Ciencias, Universidad de A Coruña, Campus de A Zapateira s/n, 15071 A Coruña, Spain. Electronic address: angeles.cid@udc.es.
authors.forename: Ángeles
authors.initials: Á
authors.lastname: Cid
mesh_terms: D001280:Atrazine / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D016825:Chlamydomonas reinhardtii / Q000187:drug effects*; D005434:Flow Cytometry; D006540:Herbicides / Q000633:toxicity; D010788:Photosynthesis / Q000187:drug effects; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D006540:Herbicides; D014874:Water Pollutants, Chemical; D001280:Atrazine
keywords: Atrazine; Cytotoxicity; Flow cytometry; Microalga; RNA-Seq