title: Characterization of single nucleotide polymorphisms in the promoter region of the bovine paraoxonase 1 (PON1) gene affecting serum enzyme activity in dairy cows.
abstract: The aim of the present study was to characterize single nucleotide polymorphisms (SNPs) in the promoter region of the bovine paraoxonase 1 (PON1) gene and determine their relationship with PON1 serum activity. Blood samples were collected from 47 Holstein cows during the periparturient period. Serum PON1 was measured and DNA extracted for PCR and sequencing of the promoter region of the PON1 gene. Seven novel SNPs were identified and of these, five SNPs, at positions -105, -221, -392, -611 and -674, upstream of the start of the published mRNA sequence (NM_001046269.2), were found to be associated with variability in serum PON1 activity (P < 0.05). The SNPs at positions -221 and -611 were located in regions predicted to bind to transcription factors linked to the acute phase response.
authors.affiliation: Faculdade de Veterinária, Universidade Federal de Pelotas, Campus Capão do Leão, sn, Pelotas, RS, Brazil.
authors.forename: Pedro A S
authors.initials: PA
authors.lastname: Silveira
authors.affiliation: Faculdade de Veterinária, Universidade Federal do Pampa, BR 472 Km 592, Uruguaiana, RS, Brazil.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Schwegler
authors.affiliation: Faculdade de Veterinária, Universidade Federal de Pelotas, Campus Capão do Leão, sn, Pelotas, RS, Brazil.
authors.forename: Paula
authors.initials: P
authors.lastname: Montagner
authors.affiliation: Department of Veterinary Biomedical Sciences, University of Saskatchewan, 52 Campus Drive, Saskatoon, Canada.
authors.forename: Ana R T
authors.initials: AR
authors.lastname: Krause
authors.affiliation: Faculdade de Veterinária, Universidade Federal de Pelotas, Campus Capão do Leão, sn, Pelotas, RS, Brazil.
authors.forename: Diego A V
authors.initials: DA
authors.lastname: Acosta
authors.affiliation: Faculdade de Zootecnia, Universidade Federal de Pelotas, Campus Capão do Leão, sn, Pelotas, RS, Brazil.
authors.forename: Jéssica
authors.initials: J
authors.lastname: Halfen
authors.affiliation: Faculdade de Veterinária, Universidade Federal de Pelotas, Campus Capão do Leão, sn, Pelotas, RS, Brazil.
authors.forename: Tiago
authors.initials: T
authors.lastname: Garlet
authors.affiliation: Faculdade de Nutrição, Universidade Federal de Pelotas, Rua Gomes Carneiro, 1, Pelotas, RS, Brazil.
authors.forename: Carlos C
authors.initials: CC
authors.lastname: Barros
authors.affiliation: Faculdade de Veterinária, Universidade Federal de Pelotas, Campus Capão do Leão, sn, Pelotas, RS, Brazil.
authors.forename: Marcio N
authors.initials: MN
authors.lastname: Corrêa
authors.affiliation: Faculdade de Nutrição, Universidade Federal de Pelotas, Rua Gomes Carneiro, 1, Pelotas, RS, Brazil. Electronic address: augusto.schneider@ufpel.edu.br.
authors.forename: Augusto
authors.initials: A
authors.lastname: Schneider
mesh_terms: D000818:Animals; D043303:Aryldialkylphosphatase / Q000097:blood / Q000235:genetics*; D002417:Cattle / Q000378:metabolism*; D003612:Dairying; D005260:Female; D020641:Polymorphism, Single Nucleotide; D049590:Postpartum Period; D011401:Promoter Regions, Genetic
chemical_list: D043303:Aryldialkylphosphatase
keywords: Cattle; Inflammation; Paraoxonase 1; Polymorphisms