title: Motility of Fasciola hepatica miracidia assessed with a computer-assisted sperm analyser.
abstract: The motility parameters of Fasciola hepatica miracidia were assessed at different temperatures and times post-hatching using computer-assisted sperm analysis. Eggs were incubated at 22 °C or 25 °C for 14 days. Five motion parameters were evaluated at different incubation temperatures up to 10 h post-hatching. No differences were observed in the percentage that hatched after incubation at the two different temperatures. However, the straight-line velocity of miracidia following incubation at 22 °C was significantly different from that observed at 25 °C (P< 0.01). All miracidium motion parameters at different post-hatching temperatures showed an overall decrease at the end of the experiment. Those miracidia hatching from eggs incubated at 25 °C had a higher velocity of 1673.3 μm/s compared with 1553.3 μm/s at 22 °C. Velocity parameters increased as the post-hatching temperature increased from 22 °C to 37 °C.
authors.affiliation: Facultad de Medicina Veterinaria y Zootecnia, Benemérita Universidad Autónoma de Puebla,Tecamachalco Puebla,México.
authors.forename: A
authors.initials: A
authors.lastname: Villa-Mancera
authors.affiliation: Facultad de Medicina Veterinaria y Zootecnia, Benemérita Universidad Autónoma de Puebla,Tecamachalco Puebla,México.
authors.forename: A
authors.initials: A
authors.lastname: Reynoso-Palomar
authors.affiliation: Unidad Académica de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Guerrero, Ciudad Altamirano,Guerrero,México.
authors.forename: J
authors.initials: J
authors.lastname: Olivares-Pérez
authors.affiliation: Departamento de Parasitología,Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México,México, D.F.México.
authors.forename: S
authors.initials: S
authors.lastname: Ortega-Vargas
authors.affiliation: Departamento de Parasitología,Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México,México, D.F.México.
authors.forename: I
authors.initials: I
authors.lastname: Cruz-Mendoza
authors.affiliation: Departamento de Parasitología,Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México,México, D.F.México.
authors.forename: H
authors.initials: H
authors.lastname: Quiroz-Romero
mesh_terms: D000818:Animals; D005210:Fasciola hepatica / Q000502:physiology*; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation*; D009068:Movement