title: Biochemical Responses of Anodized Titanium Implants with a Poly(lactide-co-glycolide)/Bone Morphogenic Protein-2 Submicron Particle Coating. Part 1: An In Vitro Study.
abstract: OBJECTIVE
  This study was conducted to examine the effects of coating poly(D,L-lactide-co-glycolide) (PLGA)/recombinant human bone morphogenetic protein-2 (rhBMP-2) submicron particles by electrospray onto titanium (Ti) implants on the proliferation and differentiation capacity of mesenchymal stem cells. In addition, the duration of rhBMP-2 release was investigated.
  
  
  METHODS
  Ti disks were fabricated and divided into four groups as follows. Group C (control) was anodized at 300 V, group P was anodized and then coated with 0.2% PLGA, group B1 was anodized and then coated with PLGA/rhBMP-2 submicron particles at 200 ng per disk, and group B2 was anodized and then coated with PLGA/rhBMP-2 submicron particles at 600 ng per disk. The topography of the PLGA/rhBMP-2-coated Ti surfaces was examined, and proliferation assays, alkaline phosphatase activity tests, and rhBMP-2 releasing tests were conducted in vitro.
  
  
  RESULTS
  There was no difference in the roughness of control Ti disks and Ti disks coated with PLGA/rhBMP-2 submicron particles by electrospray. The proliferation of mesenchymal stem cells increased over time; at 7 days, cell proliferation on the Ti disks coated with rhBMP-2 was significantly higher than the other groups. Anodized Ti disks coated with PLGA/rhBMP-2 (groups B1 and B2) released rhBMP-2 for approximately 21 days. In accordance with the amount of rhBMP-2 released from the PLGA/rhBMP-2 construct, the differentiation capacity of mesenchymal stem cells on the PLGA/rhBMP-2-coated disks was increased significantly.
  
  
  CONCLUSIONS
  Submicron PLGA/rhBMP-2 coating on Ti implants by electrospray facilitated cell proliferation and differentiation, which is important for early healing and integration of implants.
authors.forename: Soo-Yeon
authors.initials: SY
authors.lastname: Yoo
authors.forename: Seong-Kyun
authors.initials: SK
authors.lastname: Kim
authors.forename: Seong-Joo
authors.initials: SJ
authors.lastname: Heo
authors.forename: Jai-Young
authors.initials: JY
authors.lastname: Koak
authors.forename: Joo-Hee
authors.initials: JH
authors.lastname: Lee
authors.forename: Ji-Man
authors.initials: JM
authors.lastname: Park
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D055396:Bone Morphogenetic Protein 2 / Q000032:analysis / Q000737:chemistry*; D002454:Cell Differentiation; D049109:Cell Proliferation; D015921:Dental Implants; D006801:Humans; D066298:In Vitro Techniques / Q000379:methods; D019344:Lactic Acid / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D011098:Polyglactin 910 / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011994:Recombinant Proteins / Q000032:analysis / Q000737:chemistry; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*; D016212:Transforming Growth Factor beta / Q000032:analysis / Q000737:chemistry*
chemical_list: D055396:Bone Morphogenetic Protein 2; D015921:Dental Implants; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D011098:Polyglactin 910; D014025:Titanium; D000469:Alkaline Phosphatase