title: Overexpression of miR-100 inhibits growth of osteosarcoma through FGFR3.
abstract: Osteosarcoma (OS) is a prevalent, fast growing cancer. Identification of molecular regulation of OS growth may result in development of a novel therapy. Previous studies have highlighted a role of microRNAs (miRNAs) in the regulation of the carcinogenesis of OS, whereas the underlying mechanisms are not completely understood. Moreover, a role of miR-100 in the growth control of OS is not clear. Here we reported significantly higher levels of fibroblast growth factor receptor 3 (FGFR3) and significantly lower levels of miR-100 in the OS specimen, compared to those in the paired normal bone tissues. Bioinformatics analysis and luciferase reporter assay suggest that miR-100 binds to the 3'UTR of FGFR3 mRNA to prevent its translation. To prove it, we modified miR-100 levels in OS cells. We found that overexpression of miR-100 in OS cells decreased FGFR3 protein levels, whereas inhibition of miR-100 increased FGFR3 protein levels, without affecting FGFR3 transcripts. Moreover, overexpression of miR-100 suppressed the OS growth in vitro and in vivo, while inhibition of miR-100 significantly increased OS growth. Taken together, our data demonstrate that miR-100 may inhibit the growth of OS through FGFR3.
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Liaoning Medical University, 5-2, Renmin Street, Jinzhou, 121000, China.
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Bi
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, 121000, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Jing
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Liaoning Medical University, 5-2, Renmin Street, Jinzhou, 121000, China. cao_yang15@163.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Cao
mesh_terms: D017209:Apoptosis / Q000235:genetics; D063646:Carcinogenesis / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis; D051498:Receptor, Fibroblast Growth Factor, Type 3 / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C544266:MIRN100 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; C496365:FGFR3 protein, human; D051498:Receptor, Fibroblast Growth Factor, Type 3
keywords: Fibroblast growth factor receptor 3 (FGFR3); Osteosarcoma (OS); miR-100