title: Clinical outcomes and genetic expression profile in human liver graft dysfunction during ischemia/reperfusion injury.
abstract: BACKGROUND
  This study aims to compare the molecular gene expression during ischemia reperfusion injury. Several surgical times were considered: in the beginning of the harvesting (T0), at the end of the cold ischemia period (T1), and after reperfusion (T2) and compared with graft dysfunction after liver transplant (OLT).
  
  
  METHODS
  We studied 54 patients undergoing OLT. Clinical, laboratory data, and histologic data (Suzuki classification) as well as the Survival Outcomes Following Liver Transplantation (SOFT) score were used and compared with the molecular gene expression of the following genes: Interleukin (IL)-1b, IL-6, tumor necrosis factor-α, perforin, E-selectin (SELE), Fas-ligand, granzyme B, heme oxygenase-1, and nitric oxide synthetase.
  
  
  RESULTS
  Fifteen patients presented with graft dysfunction according to SOFT criteria. No relevant data were obtained by comparing the variables graft dysfunction and histologic variables. We observed a statistically significant relation between SELE at T0 (P = .013) and IL-1β at T0 (P = .028) and early graft dysfunction.
  
  
  CONCLUSIONS
  We conclude that several genetically determined proinflammatory expressions may play a critical role in the development of graft dysfunction after OLT.
authors.affiliation: Centro Hepatobiliopancreático e de Transplantação, Hospital Curry Cabral, Universidade Nova de Lisboa, Lisboa, Portugal. Electronic address: fusilis@gmail.com.
authors.forename: J
authors.initials: J
authors.lastname: Paulino
authors.affiliation: Centro Hepatobiliopancreático e de Transplantação, Hospital Curry Cabral, Universidade Nova de Lisboa, Lisboa, Portugal.
authors.forename: E
authors.initials: E
authors.lastname: Vigia
authors.affiliation: CEDOC, Faculdade de Ciências Médicas, Universidade Nova de Lisboa, Lisboa, Portugal.
authors.forename: P
authors.initials: P
authors.lastname: Marcelino
authors.affiliation: Centro de Histocompatibilidade do Sul, Universidade Nova de Lisboa, Lisboa, Portugal.
authors.forename: O
authors.initials: O
authors.lastname: Abade
authors.affiliation: Instituto Gulbenkian de Ciência, Oeiras, Portugal.
authors.forename: J
authors.initials: J
authors.lastname: Sobral
authors.affiliation: Centro de Histocompatibilidade do Sul, Universidade Nova de Lisboa, Lisboa, Portugal.
authors.forename: D
authors.initials: D
authors.lastname: Ligeiro
authors.affiliation: Serviço de Anatomia Patológica, Hospital Curry Cabral, Universidade Nova de Lisboa, Lisboa, Portugal.
authors.forename: A
authors.initials: A
authors.lastname: Carvalho
authors.affiliation: Departamento de Bioestatística, Universidade Nova de Lisboa, Lisboa, Portugal.
authors.forename: M
authors.initials: M
authors.lastname: Alves
authors.affiliation: Departamento de Bioestatística, Universidade Nova de Lisboa, Lisboa, Portugal.
authors.forename: A L
authors.initials: AL
authors.lastname: Papoila
authors.affiliation: Centro de Histocompatibilidade do Sul, Universidade Nova de Lisboa, Lisboa, Portugal.
authors.forename: H
authors.initials: H
authors.lastname: Trindade
authors.affiliation: Centro Hepatobiliopancreático e de Transplantação, Hospital Curry Cabral, Universidade Nova de Lisboa, Lisboa, Portugal.
authors.forename: E
authors.initials: E
authors.lastname: Barroso
mesh_terms: D050377:Cold Ischemia / Q000379:methods*; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation; D005819:Genetic Markers / Q000235:genetics*; D006801:Humans; D008107:Liver Diseases / Q000235:genetics* / Q000378:metabolism / Q000601:surgery; D016031:Liver Transplantation; D008297:Male; D046228:Microarray Analysis; D008875:Middle Aged; D012313:RNA / Q000235:genetics*; D015427:Reperfusion Injury / Q000235:genetics* / Q000378:metabolism; D012189:Retrospective Studies; D019737:Transplants / Q000378:metabolism / Q000473:pathology
chemical_list: D005819:Genetic Markers; D012313:RNA