title: Profiling of urinary proteins in Karan Fries cows reveals more than 1550 proteins.
abstract: Urine is a non-invasive source of biological fluid, which reflects the physiological status of the mammals. We have profiled the cow urinary proteome and analyzed its functional significance. The urine collected from three healthy cows was concentrated by diafiltration (DF) followed by protein extraction using three methods, namely methanol, acetone, and ammonium sulphate (AS) precipitation and Proteo Spin urine concentration kit (PS). The quality of the protein was assessed by two-dimensional gel electrophoresis (2DE). In-gel digestion method revealed more proteins (1191) in comparison to in-solution digestion method (541). Collectively, 938, 606 and 444 proteins were identified in LC-MS/MS after in-gel and in-solution tryptic digestion of proteins prepared by AS, PS and DF methods, respectively resulting in identification of a total of 1564 proteins. Gene ontology (GO) using Panther7.0 grouped the majority of the proteins into cytoplasmic (location), catalytic activity (function), and metabolism (biological processes), while Cytoscape grouped proteins into complement and coagulation cascades; protease inhibitor activity and wound healing. Functional significance of few selected proteins seems to play important role in their physiology. Comparative analysis with human urine revealed 315 overlapping proteins. This study reports for the first time evidence of more than 1550 proteins in urine of healthy cow donors. This article is part of a Special Issue entitled: Proteomics in India.
authors.affiliation: National Dairy Research Institute, Karnal 132001, India.
authors.forename: Shveta
authors.initials: S
authors.lastname: Bathla
authors.affiliation: National Dairy Research Institute, Karnal 132001, India.
authors.forename: Preeti
authors.initials: P
authors.lastname: Rawat
authors.affiliation: National Dairy Research Institute, Karnal 132001, India.
authors.forename: Rubina
authors.initials: R
authors.lastname: Baithalu
authors.affiliation: National Dairy Research Institute, Karnal 132001, India.
authors.forename: Munna Lal
authors.initials: ML
authors.lastname: Yadav
authors.affiliation: Postgraduate Institute of Medical Education and Research, Chandigarh-160025, India.
authors.forename: Jasmine
authors.initials: J
authors.lastname: Naru
authors.affiliation: National Dairy Research Institute, Karnal 132001, India.
authors.forename: Anurag
authors.initials: A
authors.lastname: Tiwari
authors.affiliation: National Dairy Research Institute, Karnal 132001, India.
authors.forename: Sudarshan
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Central Institute for Research on Buffaloes, Hisar 125001, India.
authors.forename: Ashok K
authors.initials: AK
authors.lastname: Balhara
authors.affiliation: National Dairy Research Institute, Karnal 132001, India.
authors.forename: Surender
authors.initials: S
authors.lastname: Singh
authors.affiliation: National Dairy Research Institute, Karnal 132001, India.
authors.forename: Suman
authors.initials: S
authors.lastname: Chaudhary
authors.affiliation: Central Institute for Research on Buffaloes, Hisar 125001, India.
authors.forename: Rajesh
authors.initials: R
authors.lastname: Kumar
authors.affiliation: National Dairy Research Institute, Karnal 132001, India.
authors.forename: Masoud
authors.initials: M
authors.lastname: Lotfan
authors.affiliation: National Dairy Research Institute, Karnal 132001, India.
authors.forename: Pradip
authors.initials: P
authors.lastname: Behare
authors.affiliation: Central Institute for Research on Buffaloes, Hisar 125001, India.
authors.forename: Sushil K
authors.initials: SK
authors.lastname: Phulia
authors.affiliation: National Dairy Research Institute, Karnal 132001, India.
authors.forename: Tushar K
authors.initials: TK
authors.lastname: Mohanty
authors.affiliation: National Dairy Research Institute, Karnal 132001, India.
authors.forename: Jai K
authors.initials: JK
authors.lastname: Kaushik
authors.affiliation: Bruker Daltonics, Bangalore, India.
authors.forename: Shivramaiah
authors.initials: S
authors.lastname: Nallapeta
authors.affiliation: Central Institute for Research on Buffaloes, Hisar 125001, India.
authors.forename: Inderjeet
authors.initials: I
authors.lastname: Singh
authors.affiliation: Indian Institute of Technology, Roorkee 247667, India.
authors.forename: Srinivas K
authors.initials: SK
authors.lastname: Ambatipudi
authors.affiliation: National Dairy Research Institute, Karnal 132001, India. Electronic address: ashokmohanty1@gmail.com.
authors.forename: Ashok K
authors.initials: AK
authors.lastname: Mohanty
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000652:urine*; D006801:Humans; D011506:Proteins / Q000378:metabolism*; D011507:Proteinuria / Q000652:urine* / Q000662:veterinary; D040901:Proteomics
chemical_list: D011506:Proteins
keywords: Bovine; In-gel; In-solution; Profiling; Proteome; Shotgun proteomics; Urine