title: Acceleration of Angiogenesis after Paravasal Injection of Mesenchymal Stem Cells at the Site of Modeled Venous Thrombosis.
abstract: The results of transplantation of autologous bone marrow multipotent mesenchymal stem cells carrying GFP gene and labeled with cell nucleus-specific dye DAPI near the thrombosed vein in rat hind limb were studied by methods of luminescent microscopy. It was demonstrated that autologous multipotent mesenchymal stem cells participate in the formation of granulations at the site of surgery. The blood fl ow in the thrombosed great vein was always restored through thrombolysis. We observed no signs of incorporation of the transplanted cells into the wall of the great vessel, clot recanalization, or formation of collaterals. Small branches of the great vein in the affected region were also thrombosed. The blood fl ow in these branches was always restored with participation of the transplanted cells or through clot recanalization or through obliteration of the thrombosed vessels and formation of new vessels. The transplanted cells and structures formed by them were gradually replaced by the recipient cells.
authors.affiliation: Center of New Medical Technologies, Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia, imai@mail.ru.
authors.forename: I V
authors.initials: IV
authors.lastname: Maiborodin
authors.forename: V V
authors.initials: VV
authors.lastname: Morozov
authors.forename: Ya V
authors.initials: YV
authors.lastname: Markevich
authors.forename: V A
authors.initials: VA
authors.lastname: Matveeva
authors.forename: L V
authors.initials: LV
authors.lastname: Artem'eva
authors.forename: A L
authors.initials: AL
authors.lastname: Matveev
authors.forename: G A
authors.initials: GA
authors.lastname: Chastikin
authors.forename: Yu V
authors.initials: YV
authors.lastname: Seryapina
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation; D064987:Cell- and Tissue-Based Therapy / Q000379:methods*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D049452:Green Fluorescent Proteins; D006614:Hindlimb / Q000098:blood supply*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology; D018919:Neovascularization, Physiologic / Q000502:physiology*; D051381:Rats; D020246:Venous Thrombosis / Q000628:therapy*
chemical_list: D049452:Green Fluorescent Proteins