title: Genetic characterization of peste des petits ruminants virus, Turkey, 2009-2013.
abstract: Peste des petits ruminants is an endemic disease of small ruminants in Turkey and vaccination has been the method of control but sporadic outbreaks have been reported. This study was carried out to characterize the local peste des petits ruminants virus (PPRV) by sequencing fusion (F) protein and nucleoprotein (N) gene segments and phylogenetic analysis, so as to focus on genetic variation in the field viruses. Samples were collected from sheep and goats clinically suspected of having PPRV infection in Central and Mediterranean regions of Turkey during 2009-2013. Phylogenetic analysis based on the F gene sequences showed that the field isolates in the present study belong to lineage 4 with other Middle East isolates. While N gene sequences revealed a different pattern, the field isolates in the present study clustered with previous Turkish isolates, which probably represents the true picture of molecular epidemiology for PPRV.
authors.affiliation: Molecular Microbiology Laboratory, Veterinary Control Institute, Meram, 42080 Konya, Turkey. Electronic address: murat.sevik@gthb.gov.tr.
authors.forename: Murat
authors.initials: M
authors.lastname: Şevik
authors.affiliation: Viral Diagnostics Laboratory, Veterinary Control Institute, Pendik, 34890 İstanbul, Turkey. Electronic address: saitvet@gmail.com.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Sait
mesh_terms: D000818:Animals; D001483:Base Sequence; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D014644:Genetic Variation; D015511:Goat Diseases / Q000821:virology*; D006041:Goats; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009698:Nucleoproteins / Q000235:genetics; D029021:Peste-des-Petits-Ruminants / Q000821:virology*; D018107:Peste-des-petits-ruminants virus / Q000235:genetics*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary; D012756:Sheep; D012757:Sheep Diseases / Q000821:virology*; D034561:Sheep, Domestic; D014421:Turkey; D014760:Viral Fusion Proteins / Q000235:genetics
chemical_list: D017931:DNA Primers; D009698:Nucleoproteins; D014760:Viral Fusion Proteins
keywords: F gene; Genetic characterization; N gene; Peste des petits ruminants; Small ruminants; Turkey