title: Microarray analysis of the in vitro granulomatous response to Mycobacterium tuberculosis H37Ra.
abstract: BACKGROUND
  The hallmark of tuberculosis is the granuloma, an organized cellular accumulation playing a key role in host defense against Mycobacterium tuberculosis. These structures sequester and contain mycobacterial cells preventing active disease, while long term maintenance of granulomas leads to latent disease. Clear understanding on mechanisms involved in granuloma formation and maintenance is lacking.
  
  
  OBJECTIVE
  To monitor granuloma formation and to determine gene expression profiles induced during the granulomatous response to M. tuberculosis (H37Ra).
  
  
  METHODS
  We used a previously characterized in vitro human model. Cellular aggregation was followed daily with microscopy and Wright staining for 5 days. Granulomas were collected at 24 h, RNA extracted and hybridized to Affymetrix human microarrays.
  
  
  RESULTS
  Daily microscopic examination revealed gradual formation of granulomas in response to mycobacterial infection. Granulomatous structures persisted for 96 h, and then began to disappear.
  
  
  CONCLUSIONS
  Microarray analysis identified genes in the innate immune response and antigen presentation pathways activated during the in vitro granulomatous response to live mycobacterial cells, revealing very early changes in gene expression of the human granulomatous response.
authors.affiliation: Research Group of Genetics and Molecular Biology. University of Cartagena. Cartagena, Colombia.
authors.forename: Niradiz
authors.initials: N
authors.lastname: Reyes
authors.affiliation: Research Group of Genetics and Molecular Biology. University of Cartagena. Cartagena, Colombia.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Bettin
authors.affiliation: Westchester Medical Center, Valhalla, NY, United States.
authors.forename: Ismael
authors.initials: I
authors.lastname: Reyes
authors.affiliation: New York Medical College, Valhalla, NY, United States.
authors.forename: Jan
authors.initials: J
authors.lastname: Geliebter
mesh_terms: D002449:Cell Aggregation; D005786:Gene Expression Regulation; D006099:Granuloma / Q000235:genetics / Q000382:microbiology / Q000473:pathology*; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics; D046228:Microarray Analysis / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D014376:Tuberculosis / Q000235:genetics / Q000382:microbiology / Q000473:pathology*
keywords: Mycobacterium tuberculosis; chemokines; granuloma; oligonucleotide microarrays