title: Development of a Rapid and Accurate Identification Method for Citrobacter Species Isolated from Pork Products Using a Matrix-Assisted Laser-Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS).
abstract: Previous detection methods for Citrobacter are considered time consuming and laborious. In this study, we have developed a rapid and accurate detection method for Citrobacter species in pork products, using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). A total of 35 Citrobacter strains were isolated from 30 pork products and identified by both MALDI-TOF MS and 16S rRNA gene sequencing approaches. All isolates were identified to the species level by the MALDI-TOF MS, while 16S rRNA gene sequencing results could not discriminate them clearly. These results confirmed that MALDI-TOF MS is a more accurate and rapid detection method for the identification of Citrobacter species.
authors.affiliation: Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Republic of Korea.
authors.forename: Hye-Lim
authors.initials: HL
authors.lastname: Kwak
authors.affiliation: Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Republic of Korea.
authors.forename: Sun-Kyung
authors.initials: SK
authors.lastname: Han
authors.affiliation: Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Republic of Korea.
authors.forename: Sunghoon
authors.initials: S
authors.lastname: Park
authors.affiliation: Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Republic of Korea.|Center for Food Safety, Department of Food Science, University of Arkansas, Fayetteville, AR 72704, USA.
authors.forename: Si Hong
authors.initials: SH
authors.lastname: Park
authors.affiliation: Department of Food & Biotechnology, Hankyong National University, Anseong 456-749, Republic of Korea.
authors.forename: Jae-Yong
authors.initials: JY
authors.lastname: Shim
authors.affiliation: National Institute of Animal Science, Rural Development Administration, Suwon 441-706, Republic of Korea.
authors.forename: Mihwa
authors.initials: M
authors.lastname: Oh
authors.affiliation: Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Republic of Korea.|Center for Food Safety, Department of Food Science, University of Arkansas, Fayetteville, AR 72704, USA.
authors.forename: Steven C
authors.initials: SC
authors.lastname: Ricke
authors.affiliation: Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Republic of Korea.
authors.forename: Hae-Yeong
authors.initials: HY
authors.lastname: Kim
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D002954:Citrobacter / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D000069466:Red Meat / Q000382:microbiology*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013997:Time Factors
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
keywords: Citrobacter sp.; MALDI-TOF MS; identification; pork products