title: The Structural Variation Is Associated with the Embryonic Lethality of a Novel Red Egg Mutant Fuyin-lre of Silkworm, Bombyx mori.
abstract: Bombyx mori presents several types of egg color mutations, all of which have been extensively discussed in sericulture. While the red egg mutation has been previously observed, lethal red-egg mutants have not been reported. In the present work, the red egg mutant Fuyin-lre (Fuyin-lethal red egg) was discovered from the Fuyin germplasm resource of B. mori. This mutant features red-colored eggs and embryonic lethality. Genetic analysis showed that Fuyin-lre follows recessive inheritance, with the red egg gene re governing the egg color, and the embryonic lethality of Fuyin-lre may be caused by mutations of other genes closely linked to re. Digital gene expression (DGE) was employed to compare the transcription profiles of Fuyin and Fuyin-lre eggs after 24 and 48 h of incubation. A total of 48 differentially expressed genes followed the same expression patterns in both groups at both time points (FDR < 0.01 and log 2 Ratio ≥ 1). Further analyses indicated that 8 out of the 48 genes (including re) were closely linked to re. These 8 genes were highly expressed in wild-type Fuyin and the red egg mutant re but showed nearly absent expression in Fuyin-lre. Sequencing of the re gene confirmed that the re gene itself does not induce embryonic lethality, and structure analysis showed that the structural variation of the region where the 8 genes were located may be associated with the embryonic lethality of Fuyin-lre. The present work provides a good foundation for future studies on the mechanism of embryonic lethality and embryonic development in Fuyin-lre.
authors.affiliation: The Sericultural and Apicultural Research Institute, Yunnan Academy of Agricultural Sciences, Mengzi Yunnan, China.
authors.forename: Anli
authors.initials: A
authors.lastname: Chen
authors.affiliation: The Sericultural and Apicultural Research Institute, Yunnan Academy of Agricultural Sciences, Mengzi Yunnan, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Liao
authors.affiliation: The Sericultural and Apicultural Research Institute, Yunnan Academy of Agricultural Sciences, Mengzi Yunnan, China.
authors.forename: Qiongyan
authors.initials: Q
authors.lastname: Li
authors.affiliation: Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang Jiangsu, China.
authors.forename: Qiaoling
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: The Sericultural and Apicultural Research Institute, Yunnan Academy of Agricultural Sciences, Mengzi Yunnan, China.
authors.forename: Weike
authors.initials: W
authors.lastname: Yang
authors.affiliation: The Sericultural and Apicultural Research Institute, Yunnan Academy of Agricultural Sciences, Mengzi Yunnan, China.
authors.forename: Shuifen
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang Jiangsu, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wu
authors.affiliation: Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang Jiangsu, China.
authors.forename: Rongfan
authors.initials: R
authors.lastname: He
authors.affiliation: The Sericultural and Apicultural Research Institute, Yunnan Academy of Agricultural Sciences, Mengzi Yunnan, China.
authors.forename: Zhanpeng
authors.initials: Z
authors.lastname: Dong
authors.affiliation: The Sericultural and Apicultural Research Institute, Yunnan Academy of Agricultural Sciences, Mengzi Yunnan, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Huang
mesh_terms: D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000196:embryology* / Q000235:genetics* / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000196:embryology*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D019476:Insect Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D010063:Ovum / Q000378:metabolism*; D010858:Pigmentation / Q000235:genetics*
chemical_list: D019476:Insect Proteins