title: Triacylglycerol Analysis in Human Milk and Other Mammalian Species: Small-Scale Sample Preparation, Characterization, and Statistical Classification Using HPLC-ELSD Profiles.
abstract: In this work, a method for the separation of triacylglycerols (TAGs) present in human milk and from other mammalian species by reversed-phase high-performance liquid chromatography using a core-shell particle packed column with UV and evaporative light-scattering detectors is described. Under optimal conditions, a mobile phase containing acetonitrile/n-pentanol at 10 °C gave an excellent resolution among more than 50 TAG peaks. A small-scale method for fat extraction in these milks (particularly of interest for human milk samples) using minimal amounts of sample and reagents was also developed. The proposed extraction protocol and the traditional method were compared, giving similar results, with respect to the total fat and relative TAG contents. Finally, a statistical study based on linear discriminant analysis on the TAG composition of different types of milks (human, cow, sheep, and goat) was carried out to differentiate the samples according to their mammalian origin.
authors.affiliation: †Department of Analytical Chemistry, Faculty of Chemistry, University of Valencia, C. Dr. Moliner 50, E-46100 Burjassot, Valencia, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Ten-Doménech
authors.affiliation: §Research Institute for Pesticides and Water, University Jaume I, E-12071 Castellón, Spain.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Beltrán-Iturat
authors.affiliation: †Department of Analytical Chemistry, Faculty of Chemistry, University of Valencia, C. Dr. Moliner 50, E-46100 Burjassot, Valencia, Spain.
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Herrero-Martínez
authors.affiliation: §Research Institute for Pesticides and Water, University Jaume I, E-12071 Castellón, Spain.
authors.forename: Juan Vicente
authors.initials: JV
authors.lastname: Sancho-Llopis
authors.affiliation: †Department of Analytical Chemistry, Faculty of Chemistry, University of Valencia, C. Dr. Moliner 50, E-46100 Burjassot, Valencia, Spain.
authors.forename: Ernesto Francisco
authors.initials: EF
authors.lastname: Simó-Alfonso
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000295:instrumentation / Q000379:methods; D016002:Discriminant Analysis; D005260:Female; D006041:Goats; D006801:Humans; D013058:Mass Spectrometry; D008892:Milk / Q000737:chemistry*; D008895:Milk, Human / Q000737:chemistry*; D012756:Sheep; D014280:Triglycerides / Q000032:analysis*
chemical_list: D014280:Triglycerides
keywords: HPLC-ELSD; fat extraction; human milk; linear discriminant analysis; mammalian milk triacylglycerols