title: Morphology and Molecular Phylogeny of Two New Brackish Water Ciliates of Bakuella (Ciliophora: Urostylida: Bakuellidae) from South Korea.
abstract: Two new species of Bakuella, B. (B.) incheonensis n. sp. and B. (Pseudobakuella) litoralis n. sp., were discovered in brackish water near Aamdo Shore Park, Incheon, South Korea. We conducted a morphological study based on live observations and protargol-impregnated specimens, and a molecular analysis using nuclear SSU rRNA gene sequences. Bakuella (B.) incheonensis is diagnosed by: body size 70-105 × 20-40 μm in vivo, 21-25 adoral membranelles, three or four frontoterminal cirri, midventral complex composed of 7-10 midventral pairs with one or two rows and terminating at about 62% of body length, 20-28 left and 25-32 right marginal cirri, 58-87 macronuclear nodules, and yellowish cortical granules. Bakuella (Pseudobakuella) litoralis is diagnosed by: body size 90-125 × 30-40 μm in vivo, 25-33 adoral membranelles, 3-5 buccal cirri, midventral complex composed 10-15 midventral pairs with one or two rows and terminated at 70% of body length, one or two pretransverse cirri, 3-6 transverse cirri, 26-39 left and 29-47 right marginal cirri, 49-84 macronuclear nodules, and two types of cortical granules. Molecular phylogeny using SSU rRNA gene sequences shows a nonmonophyletic relationship among Bakuella species and emphasizes the need for further morphogenetic studies of this genus and other related hypotrichs.
authors.affiliation: Department of Biological Sciences, Inha University, Incheon, 402-751, South Korea.
authors.forename: Euna
authors.initials: E
authors.lastname: Jo
authors.affiliation: Department of Biological Sciences, Inha University, Incheon, 402-751, South Korea.
authors.forename: Jae-Ho
authors.initials: JH
authors.lastname: Jung
authors.affiliation: Department of Biological Sciences, Inha University, Incheon, 402-751, South Korea.
authors.forename: Gi-Sik
authors.initials: GS
authors.lastname: Min
mesh_terms: D016798:Ciliophora / Q000145:classification* / Q000166:cytology* / Q000235:genetics / Q000302:isolation & purification; D016054:DNA, Protozoan / Q000235:genetics; D020459:Genes, rRNA / Q000235:genetics; D016810:Hypotrichida / Q000235:genetics; D008969:Molecular Sequence Data; D009024:Morphogenesis; D010802:Phylogeny; D056910:Republic of Korea; D000068357:Saline Waters; D012623:Seawater / Q000469:parasitology; D013045:Species Specificity
chemical_list: D016054:DNA, Protozoan
keywords: Bakuella incheonensis; Bakuella litoralis; SSU rRNA gene; ciliature; new species