title: Quantitative Analysis of Intra-chromosomal Contacts: The 3C-qPCR Method.
abstract: The chromosome conformation capture (3C) technique is fundamental to many population-based methods investigating chromatin dynamics and organization in eukaryotes. Here, we provide a modified quantitative 3C (3C-qPCR) protocol for improved quantitative analyses of intra-chromosomal contacts. We also describe an algorithm for data normalization which allows more accurate comparisons between contact profiles.
authors.affiliation: Institut de Génétique Moléculaire de Montpellier, UMR5535, CNRS, Université de Montpellier, 1919 Route de Mende, 34293, Montpellier Cedex 5, France.
authors.forename: Vuthy
authors.initials: V
authors.lastname: Ea
authors.affiliation: Institut de Génétique Moléculaire de Montpellier, UMR5535, CNRS, Université de Montpellier, 1919 Route de Mende, 34293, Montpellier Cedex 5, France.|Inserm UMR1103, CNRS UMR6293, F-63001 Clermont-Ferrand, France and Clermont Université, Université d'Auvergne, Laboratoire GReD, BP 10448, F-63000, Clermont-Ferrand, France.
authors.forename: Franck
authors.initials: F
authors.lastname: Court
authors.affiliation: Institut de Génétique Moléculaire de Montpellier, UMR5535, CNRS, Université de Montpellier, 1919 Route de Mende, 34293, Montpellier Cedex 5, France. forne@igmm.cnrs.fr.
authors.forename: Thierry
authors.initials: T
authors.lastname: Forné
mesh_terms: D002843:Chromatin / Q000737:chemistry*; D002874:Chromosome Mapping / Q000379:methods*; D002877:Chromosomes, Human / Q000737:chemistry*; D017931:DNA Primers / Q000737:chemistry; D006801:Humans; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*
chemical_list: D002843:Chromatin; D017931:DNA Primers
keywords: Chromatin dynamics and organization; Chromosome conformation capture; Quantitative PCR