title: Analysis of the metabolic profile of parishin by ultra-performance liquid chromatography/quadrupole-time of flight mass spectrometry.
abstract: Parishin is a dominant active ingredient originating from Gastrodia elata Blume, and has good neuroprotective effects against brain disorders. In the present study, the metabolic profile of parishin by in vitro and in vivo experiments was investigated using ultra-high performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry (UHPLC/Q-TOF MS) combined with an automated MS(E) technique. By comparison with reference compounds, accurate mass measurement, the characteristic fragmentation patterns of the parent drug parishin and gastrodin and relevant bio-transformation knowledge, 14 metabolites (seven hydrolyzates and seven derivatives of gastrodin) were detected and identified in rat plasma and urine after intragastric administration of parishin, including processes of hydrolyzation, oxidation, sulfation and glucuronidation. According to the proposed metabolic pathways of parishin, in vitro hydrolytic experiments and metabolic study of gastrodin in rat plasma, it can be inferred that parishin mainly functions as a prodrug and undergoes hydrolysis before being absorbed into the blood. The hydrolyzate, mainly gastrodin, was involved in further metabolism, which was responsible for pharmacological activities of parishin. In conclusion, this work provides valuable information on parishin metabolism using a rapid and reliable UHPLC/Q-TOF MS method, which could be widely used for the metabolic investigation of natural product.
authors.affiliation: Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, 116023, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Chunlan
authors.initials: C
authors.lastname: Tang
authors.affiliation: Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, 116023, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Affiliated Zhongshan Hospital of Dalian University, Dalian, 116001, China.
authors.forename: Jiejiang
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, 116023, China.
authors.forename: Xinxin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, 116023, China.
authors.forename: Mengchun
authors.initials: M
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, 116023, China.|Beijing University of Chinese Medicine, Beijing, 100029, China.
authors.forename: Hongbin
authors.initials: H
authors.lastname: Xiao
mesh_terms: D000818:Animals; D001592:Benzyl Alcohols / Q000097:blood / Q000737:chemistry / Q000378:metabolism / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002951:Citrates / Q000008:administration & dosage / Q000097:blood / Q000737:chemistry / Q000378:metabolism / Q000652:urine; D005960:Glucosides / Q000008:administration & dosage / Q000097:blood / Q000737:chemistry / Q000378:metabolism / Q000652:urine; D020719:Glucuronides; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013431:Sulfates
chemical_list: D001592:Benzyl Alcohols; D002951:Citrates; D005960:Glucosides; D020719:Glucuronides; D013431:Sulfates; C554064:parishin C; C045345:gastrodin
keywords: UHPLC/Q-TOF MS; hydrolyzation; metabolic profile; parishin