title: Extracellular matrix-associated proteins form an integral and dynamic system during Pseudomonas aeruginosa biofilm development.
abstract: Though the essential role of extracellular matrix in biofilm development has been extensively documented, the function of matrix-associated proteins is elusive. Determining the dynamics of matrix-associated proteins would be a useful way to reveal their functions in biofilm development. Therefore, we applied iTRAQ-based quantitative proteomics to evaluate matrix-associated proteins isolated from different phases of Pseudomonas aeruginosa ATCC27853 biofilms. Among the identified 389 proteins, 54 changed their abundance significantly. The increased abundance of stress resistance and nutrient metabolism-related proteins over the period of biofilm development was consistent with the hypothesis that biofilm matrix forms micro-environments in which cells are optimally organized to resist stress and use available nutrients. Secreted proteins, including novel putative effectors of the type III secretion system were identified, suggesting that the dynamics of pathogenesis-related proteins in the matrix are associated with biofilm development. Interestingly, there was a good correlation between the abundance changes of matrix-associated proteins and their expression. Further analysis revealed complex interactions among these modulated proteins, and the mutation of selected proteins attenuated biofilm development. Collectively, this work presents the first dynamic picture of matrix-associated proteins during biofilm development, and provides evidences that the matrix-associated proteins may form an integral and well regulated system that contributes to stress resistance, nutrient acquisition, pathogenesis and the stability of the biofilm.
authors.affiliation: Division of Life Science, The Hong Kong University of Science and Technology Hong Kong, China.
authors.forename: Weipeng
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Biology, Hong Kong Baptist University Hong Kong, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A & F University Yangling, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ding
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A & F University Yangling, China.
authors.forename: Jinshui
authors.initials: J
authors.lastname: Lin
authors.affiliation: Division of Life Science, The Hong Kong University of Science and Technology Hong Kong, China.
authors.forename: Renmao
authors.initials: R
authors.lastname: Tian
authors.affiliation: Division of Life Science, The Hong Kong University of Science and Technology Hong Kong, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Lu
authors.affiliation: Division of Life Science, The Hong Kong University of Science and Technology Hong Kong, China.
authors.forename: Xiaofen
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A & F University Yangling, China.
authors.forename: Xihui
authors.initials: X
authors.lastname: Shen
authors.affiliation: Division of Life Science, The Hong Kong University of Science and Technology Hong Kong, China.
authors.forename: Pei-Yuan
authors.initials: PY
authors.lastname: Qian
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D018441:Biofilms / Q000254:growth & development*; D005109:Extracellular Matrix / Q000737:chemistry*; D006801:Humans; D020543:Proteome / Q000032:analysis*; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D020543:Proteome
keywords: Pseudomonas aeruginosa ATCC27853; biofilm; iTRAQ; matrix-associated proteins; type three secretion system