title: Generation and characterization of a novel shoulder contracture mouse model.
abstract: Frozen shoulder is a relatively common disorder that leads to severe pain and stiffness in the shoulder joint. Although this disorder is self-limiting in nature, the symptoms often persist for years, resulting in severe disability. Recent studies using human specimens and animal models have shown distinct changes in the gene expression patterns in frozen shoulder tissue, indicating that novel therapeutic intervention could be achieved by controlling the genes that are potentially involved in the development of frozen shoulder. To achieve this goal, it is imperative to develop a reliable animal joint contracture model in which gene expression can be manipulated by gene targeting and transgenic technologies. Here, we describe a novel shoulder contracture mouse model. We found that this model mimics the clinical presentation of human frozen shoulder and recapitulates the changes in the gene expression pattern and the histology of frozen shoulder and joint contracture in humans and other larger animal models. The model is highly reproducible, without any major complications. Therefore, the present model may serve as a useful tool for investigating frozen shoulder etiology and for identifying its potential target genes.
authors.affiliation: Department of Orthopedic Surgery, Keio University School of Medicine, city, Shinjuku-ku, Tokyo, 160-8582, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Oki
authors.affiliation: Department of Orthopedic Surgery, Keio University School of Medicine, city, Shinjuku-ku, Tokyo, 160-8582, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Shirasawa
authors.affiliation: Department of Anti-Aging Orthopedic Research, Keio University School of Medicine, Tokyo, 160-8582, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Yoda
authors.affiliation: Department of Orthopedic Surgery, Keio University School of Medicine, city, Shinjuku-ku, Tokyo, 160-8582, Japan.
authors.forename: Noboru
authors.initials: N
authors.lastname: Matsumura
authors.affiliation: Department of Anti-Aging Orthopedic Research, Keio University School of Medicine, Tokyo, 160-8582, Japan.
authors.forename: Takahide
authors.initials: T
authors.lastname: Tohmonda
authors.affiliation: Sato Pharmaceutical Corporation, Tokyo, 107-0051, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Yuasa
authors.affiliation: Department of Orthopedic Surgery, Keio University School of Medicine, city, Shinjuku-ku, Tokyo, 160-8582, Japan.
authors.forename: Masaya
authors.initials: M
authors.lastname: Nakamura
authors.affiliation: Department of Orthopedic Surgery, Keio University School of Medicine, city, Shinjuku-ku, Tokyo, 160-8582, Japan.
authors.forename: Morio
authors.initials: M
authors.lastname: Matsumoto
authors.affiliation: Department of Orthopedic Surgery, Keio University School of Medicine, city, Shinjuku-ku, Tokyo, 160-8582, Japan.|Department of Anti-Aging Orthopedic Research, Keio University School of Medicine, Tokyo, 160-8582, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Horiuchi
mesh_terms: D000818:Animals; D002062:Bursitis / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D017746:Joint Capsule / Q000378:metabolism; D008297:Male; D008810:Mice, Inbred C57BL; D012785:Shoulder Joint / Q000378:metabolism / Q000473:pathology
keywords: functional analysis; gene expression analysis; mouse model; shoulder contracture