title: Functional differentiation of human pluripotent stem cells on a chip.
abstract: Microengineering human "organs-on-chips" remains an open challenge. Here, we describe a robust microfluidics-based approach for the differentiation of human pluripotent stem cells directly on a chip. Extrinsic signal modulation, achieved through optimal frequency of medium delivery, can be used as a parameter for improved germ layer specification and cell differentiation. Human cardiomyocytes and hepatocytes derived on chips showed functional phenotypes and responses to temporally defined drug treatments.
authors.affiliation: 1] Department of Industrial Engineering, University of Padova, Padova, Italy. [2] Venetian Institute of Molecular Medicine, Padova, Italy.
authors.forename: Giovanni G
authors.initials: GG
authors.lastname: Giobbe
authors.affiliation: 1] Department of Industrial Engineering, University of Padova, Padova, Italy. [2] Venetian Institute of Molecular Medicine, Padova, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Michielin
authors.affiliation: 1] Department of Industrial Engineering, University of Padova, Padova, Italy. [2] Venetian Institute of Molecular Medicine, Padova, Italy.
authors.forename: Camilla
authors.initials: C
authors.lastname: Luni
authors.affiliation: 1] Department of Industrial Engineering, University of Padova, Padova, Italy. [2] Venetian Institute of Molecular Medicine, Padova, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Giulitti
authors.affiliation: 1] Department of Industrial Engineering, University of Padova, Padova, Italy. [2] Venetian Institute of Molecular Medicine, Padova, Italy.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Martewicz
authors.affiliation: Department of Molecular Medicine, University of Padova, Padova, Italy.
authors.forename: Sirio
authors.initials: S
authors.lastname: Dupont
authors.affiliation: Department of Surgery, Oncology and Gastroenterology, University of Padova, Padova, Italy.
authors.forename: Annarosa
authors.initials: A
authors.lastname: Floreani
authors.affiliation: 1] Department of Industrial Engineering, University of Padova, Padova, Italy. [2] Venetian Institute of Molecular Medicine, Padova, Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Elvassore
mesh_terms: D002454:Cell Differentiation; D053595:Embryonic Stem Cells / Q000166:cytology; D022781:Hepatocytes / Q000166:cytology; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D032383:Myocytes, Cardiac / Q000166:cytology; D039904:Pluripotent Stem Cells / Q000166:cytology*