title: Butanol production from hexoses and pentoses by fermentation of Clostridium acetobutylicum.
abstract: The present paper reports the characterization of ABE (acetone-butanol-ethanol) production by Clostridium acetobutylicum DSM 792 for sugars representative of hydrolysed lignocellulosic biomass (glucose, mannose, arabinose, xylose). The attention was focused on: the selection of an optimal medium for the simultaneous conversion of the investigated sugars; the assessment of interference-synergistic effects during the fermentation of mixtures of the investigated sugars. The synthetic medium was optimised in terms of nutritional factors: the KH2PO4-K2HPO4 concentration was increased up to 5 g/L; the MgSO4 concentration was increased up to 2 g/L; the MnSO4 concentration was increased up to 0.1 g/L; the FeSO4 concentration ranged between 0.002 and 0.01 g/L); the CaCO3 concentration was increased up to 10 g/L. The optimal concentration of the investigated factors was assessed and it varied from one sugar to another. The batch fermentations of a mixture of the four sugars highlighted their synergistic effects. Once set the initial concentration of the sugars (60 g/L), the butanol and solvent concentration increased up to 14.6 and 20.6 g/L, respectively, when the four sugars were present.
authors.affiliation: Dipartimento di Ingegneria Chimica, dei Materiali e della Produzione Industriale, Università degli Studi di Napoli Federico II, P.le V. Tecchio 80, 80125 Napoli, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Raganati
authors.affiliation: Dipartimento di Ingegneria Chimica, dei Materiali e della Produzione Industriale, Università degli Studi di Napoli Federico II, P.le V. Tecchio 80, 80125 Napoli, Italy. Electronic address: giolivie@unina.it.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Olivieri
authors.affiliation: Department of Life Sciences and Technology / Bioprocess Engineering, Beuth University of Applied Sciences Berlin, Seestrasse 64, 13347 Berlin, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Götz
authors.affiliation: Dipartimento di Ingegneria Chimica, dei Materiali e della Produzione Industriale, Università degli Studi di Napoli Federico II, P.le V. Tecchio 80, 80125 Napoli, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Marzocchella
authors.affiliation: Dipartimento di Ingegneria Chimica, dei Materiali e della Produzione Industriale, Università degli Studi di Napoli Federico II, P.le V. Tecchio 80, 80125 Napoli, Italy.
authors.forename: Piero
authors.initials: P
authors.lastname: Salatino
mesh_terms: D061249:Batch Cell Culture Techniques; D001711:Biotransformation; D000440:Butanols / Q000378:metabolism*; D046969:Clostridium acetobutylicum; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D006601:Hexoses / Q000378:metabolism*; D010429:Pentoses / Q000378:metabolism*
chemical_list: D000440:Butanols; D003470:Culture Media; D006601:Hexoses; D010429:Pentoses
keywords: Batch fermentation; Clostridium acetobutylicum; Lignocellulosic hydrolyzate; Medium optimization; Sugar mixtures