title: Efficacious rat model displays non-toxic effect with Korean beechwood creosote: a possible antibiotic substitute.
abstract: Wood creosote, an herbal anti-diarrheal and a mixture of major volatile compounds, was tested for its non-toxicological effects, using a rat model, with the objective to use the creosote as an antibiotic substitute. A total of 30 Sprague-Dawley rats were studied to form five groups with 6 rats each. Korea beechwood creosote was supplemented into three test groups with 0.03 g/kg, 0.07 g/kg and 0.1 g/kg body weight/day without antibiotic support, along with a positive control of Apramycin sulphate (at 0.5% of the daily feed) and a negative control. Korean beechwood creosote supplementation showed no negative effect on the body weight gain in comparison to the negative and the positive control groups and the feed conversion ratio was also comparable with that of the control groups. The clinical pathology parameters studied were also under the umbrella of normal range, including liver specific enzymes, blood glucose, total protein, blood urea nitrogen (BUN), which indicated no toxic effect of creosote at the given doses. The non-hepatotoxic effect was also confirmed using hepatic damage specific molecular markers like Tim-p1, Tim-p2 and Tgf-β1. The results suggested that Korean beechwood may be used as antibiotic substitute in weanling pigs feed without any toxic effect on the body. Although the antimicrobial properties of creosote were not absolutely similar to those of apramycin sulphate, they were comparable.
authors.affiliation: Faculty of Biotechnology, Jeju National University , Jeju , Korea.
authors.forename: Anh Nguyen Thai
authors.initials: AN
authors.lastname: Quynh
authors.affiliation: Faculty of Biotechnology, Jeju National University , Jeju , Korea.
authors.forename: Neelesh
authors.initials: N
authors.lastname: Sharma
authors.affiliation: Department of Animal Resources Technology, Gyeongnam National University of Science and Technology , Jinju , Korea.
authors.forename: Kwang Keun
authors.initials: KK
authors.lastname: Cho
authors.affiliation: Department of Animal Resources Technology, Gyeongnam National University of Science and Technology , Jinju , Korea.
authors.forename: Tae Jong
authors.initials: TJ
authors.lastname: Yeo
authors.affiliation: Korea Institute for Animal Products Quality Evaluation , Anyang , Korea.
authors.forename: Ki Beom
authors.initials: KB
authors.lastname: Kim
authors.affiliation: Changjobio Research Institute, Changjobio Corporation , Jeju , Korea.
authors.forename: Chul Yon
authors.initials: CY
authors.lastname: Jeong
authors.affiliation: National Research Foundation (NRF), R&D Policy Team , Daejeon , Korea.
authors.forename: Tae Sun
authors.initials: TS
authors.lastname: Min
authors.affiliation: Swine Science & Technology Center, Gyeongnam National University of Science and Technology , Jinju , Korea.
authors.forename: Kim Jae
authors.initials: KJ
authors.lastname: Young
authors.affiliation: Faculty of Biotechnology, Jeju National University , Jeju , Korea.
authors.forename: Jin Nam
authors.initials: JN
authors.lastname: Kim
authors.affiliation: Faculty of Biotechnology, Jeju National University , Jeju , Korea.
authors.forename: Dong-Kee
authors.initials: DK
authors.lastname: Jeong
keywords: Korean beechwood creosote; antibiotic substitute; hepatotoxic markers