title: B cells Using Calcium Signaling for Specific and Rapid Detection of Escherichia coli O157:H7.
abstract: A rapid and sensitive detection technology is highly desirable for specific detection of E. coli O157:H7, one of the leading bacterial pathogens causing foodborne illness. In this study, we reported the rapid detection of E. coli O157:H7 by using calcium signaling of the B cell upon cellular membrane anchors anti-E. coli O157:H7 IgM. The binding of E. coli O157:H7 to the IgM on B cell surface activates the B cell receptor (BCR)-induced Ca(2+) signaling pathway and results in the release of Ca(2+) within seconds. The elevated intracellular Ca(2+) triggers Fura-2, a fluorescent Ca(2+) indicator, for reporting the presence of pathogens. The Fura-2 is transferred to B cells before detection. The study demonstrated that the developed B cell based biosensor was able to specifically detect E. coli O157:H7 at the low concentration within 10 min in pure culture samples. Finally, the B cell based biosensor was used for the detection of E. coli O157:H7 in ground beef samples. With its short detection time and high sensitivity at the low concentration of the target bacteria, this B cell biosensor shows promise in future application of the high throughput and rapid food detection, biosafety and environmental monitoring.
authors.affiliation: 1] College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China [2] Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, AR 72701, USA.
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, AR 72701, USA.
authors.forename: Ronghui
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA.
authors.forename: Byung-Whi
authors.initials: BW
authors.lastname: Kong
authors.affiliation: Department of Biomedical Engineering, Binghamton University-SUNY, Binghamton, NY 13902, USA.
authors.forename: Sha
authors.initials: S
authors.lastname: Jin
authors.affiliation: Department of Biomedical Engineering, Binghamton University-SUNY, Binghamton, NY 13902, USA.
authors.forename: Kaiming
authors.initials: K
authors.lastname: Ye
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Weihuan
authors.initials: W
authors.lastname: Fang
authors.affiliation: 1] College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China [2] Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, AR 72701, USA.
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D015374:Biosensing Techniques; D020013:Calcium Signaling; D002417:Cattle; D019453:Escherichia coli O157 / Q000276:immunology*; D005516:Food Microbiology; D005517:Foodborne Diseases / Q000175:diagnosis / Q000382:microbiology; D008461:Meat Products / Q000382:microbiology