title: Platelet-activating factor modulates fat storage in the liver induced by a high-refined carbohydrate-containing diet.
abstract: Hepatic diseases are comorbidities caused by obesity and are influenced by diet composition. The aim of this study was to evaluate the kinetics of metabolic and inflammatory liver dysfunction induced by a high-refined carbohydrate-containing (HC) diet and to determine how platelet-activating factor (PAF) modulates the liver lipid content of mice. BALB/c mice were fed a chow or HC diet for the following experimental periods: 1 and 3 days, 1, 2, 4, 6, 8, 10 and 12 weeks. Wild-type (WT) and PAF receptor-deficient (PAFR(-/-)) mice were fed the same diets for 8 weeks. Mice fed with HC diet showed higher triglycerides and cholesterol levels, fibrosis and inflammation in the liver. The number of neutrophils migrating into the liver was also increased in mice fed with HC diet. However, transaminase levels did not change. PAFR(-/-) mice fed with HC diet showed more steatosis, oxidative stress and higher transaminases levels associated with lower inflammation than WT mice. The consumption of HC diet altered the metabolic and inflammatory response in the liver and was worse in PAFR(-/-) mice. We suggest that PAF regulates liver lipid content and dyslipidemia, protecting the mice from lipotoxicity and liver damage.
authors.affiliation: Department of Nutrition, Nursing School, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil; Immunopharmacology, Department of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Marina Chaves
authors.initials: MC
authors.lastname: de Oliveira
authors.affiliation: Immunopharmacology, Department of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil; Department of Microbiology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Zélia
authors.initials: Z
authors.lastname: Menezes-Garcia
authors.affiliation: Immunopharmacology, Department of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil; Department of Microbiology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Raquel Duque do Nascimento
authors.initials: RD
authors.lastname: Arifa
authors.affiliation: Immunopharmacology, Department of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil; Department of Microbiology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Talles Prosperi
authors.initials: TP
authors.lastname: de Paula
authors.affiliation: Laboratory of Health Science, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil.
authors.forename: João Marcus Oliveira
authors.initials: JM
authors.lastname: Andrade
authors.affiliation: Laboratory of Health Science, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil.
authors.forename: Sérgio Henrique Sousa
authors.initials: SH
authors.lastname: Santos
authors.affiliation: Immunopharmacology, Department of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil; Department of Morphology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Gustavo Batista
authors.initials: GB
authors.lastname: de Menezes
authors.affiliation: Immunopharmacology, Department of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil; Department of Microbiology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Danielle da Glória
authors.initials: Dda G
authors.lastname: de Souza
authors.affiliation: Immunopharmacology, Department of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Mauro Martins
authors.initials: MM
authors.lastname: Teixeira
authors.affiliation: Department of Nutrition, Nursing School, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil; Immunopharmacology, Department of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. Electronic address: adaliene@gmail.com.
authors.forename: Adaliene Versiani Matos
authors.initials: AV
authors.lastname: Ferreira
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000097:blood / Q000378:metabolism; D004040:Dietary Carbohydrates / Q000009:adverse effects*; D050171:Dyslipidemias / Q000209:etiology / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D005511:Food Handling; D050356:Lipid Metabolism; D015227:Lipid Peroxidation; D008099:Liver / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D008103:Liver Cirrhosis / Q000209:etiology; D008297:Male; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D020556:Neutrophil Infiltration; D065626:Non-alcoholic Fatty Liver Disease / Q000209:etiology / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D018384:Oxidative Stress; D010972:Platelet Activating Factor / Q000378:metabolism*; D010980:Platelet Membrane Glycoproteins / Q000819:agonists* / Q000235:genetics / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000819:agonists* / Q000097:blood / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D013997:Time Factors; D014280:Triglycerides / Q000097:blood / Q000378:metabolism
chemical_list: D004040:Dietary Carbohydrates; D010972:Platelet Activating Factor; D010980:Platelet Membrane Glycoproteins; D043562:Receptors, G-Protein-Coupled; D014280:Triglycerides; C039351:platelet activating factor receptor; D002784:Cholesterol
keywords: Collagen deposition; Dyslipidemia; Inflammation; Liver; Steatosis