title: Cutting Edge: Developmental Regulation of IFN-γ Production by Mouse Neutrophil Precursor Cells.
abstract: Neutrophils are an emerging cellular source of IFN-γ, a key cytokine that mediates host defense to intracellular pathogens. Production of IFN-γ by neutrophils, in contrast to lymphoid cells, is TLR- and IL-12-independent and the events associated with IFN-γ production by neutrophils are not understood. In this study, we show that mouse neutrophils express IFN-γ during their lineage development in the bone marrow niche at the promyelocyte stage independently of microbes. IFN-γ accumulates in primary neutrophilic granules and is released upon induction of degranulation. The developmental mechanism of IFN-γ production in neutrophils arms the innate immune cells prior to infection and assures the potential for rapid release of IFN-γ upon neutrophil activation, the first step during responses to many microbial infections.
authors.affiliation: Department of Microbiology and Immunology, David H. Smith Center for Vaccine Biology and Immunology, University of Rochester, Rochester, NY 14642; Department of Immunology, University of Texas Southwestern Medical Center, Dallas, TX 75390; and.
authors.forename: Carolyn R
authors.initials: CR
authors.lastname: Sturge
authors.affiliation: Department of Microbiology and Immunology, David H. Smith Center for Vaccine Biology and Immunology, University of Rochester, Rochester, NY 14642; Department of Immunology, University of Texas Southwestern Medical Center, Dallas, TX 75390; and.
authors.forename: Elise
authors.initials: E
authors.lastname: Burger
authors.affiliation: Department of Immunology, University of Texas Southwestern Medical Center, Dallas, TX 75390; and.
authors.forename: Megan
authors.initials: M
authors.lastname: Raetz
authors.affiliation: Department of Immunology, University of Texas Southwestern Medical Center, Dallas, TX 75390; and Howard Hughes Medical Institute, Dallas, TX 75390.
authors.forename: Lora V
authors.initials: LV
authors.lastname: Hooper
authors.affiliation: Department of Microbiology and Immunology, David H. Smith Center for Vaccine Biology and Immunology, University of Rochester, Rochester, NY 14642; Department of Immunology, University of Texas Southwestern Medical Center, Dallas, TX 75390; and felix_yarovinsky@URMC.Rochester.edu.
authors.forename: Felix
authors.initials: F
authors.lastname: Yarovinsky
mesh_terms: D000818:Animals; D015550:Cell Degranulation / Q000276:immunology; D019070:Cell Lineage / Q000276:immunology; D003594:Cytoplasmic Granules / Q000276:immunology; D018507:Gene Expression Regulation, Developmental; D007113:Immunity, Innate / Q000235:genetics*; D016130:Immunophenotyping; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology*; D051379:Mice; D018345:Mice, Knockout; D018375:Neutrophil Activation; D009504:Neutrophils / Q000166:cytology / Q000276:immunology*; D015398:Signal Transduction
chemical_list: D007371:Interferon-gamma