title: The effect of Hypericum perforatum L. (St. John's Wort) on prevention of myringosclerosis after myringotomy in a rat model.
abstract: OBJECTIVE
  The purpose of this study was to identify the possible effects of Hypericum Perforatum (HP) on the prevention of experimentally induced myringosclerosis (MS).
  
  
  METHODS
  Twenty eight Wistar Albino rats were used and they were divided into four groups. Tympanic membranes of all animals were perforated and then group I had no treatment as a control group, group II had treated with olive oil only, group III had treated with HP orally and group IV had treated with HP topically.
  
  
  RESULTS
  Groups I and II showed extensive myringosclerosis in contrast to those of Groups III and IV which had significantly less changes (p<0.05). The inflammation and fibrosis in the lamina propria of the tympanic membranes of Groups I and II were found to be significantly more pronounced (p<0.05). The tympanic membranes were found to be significantly thinner in Groups III and IV when compared with Groups I and II (p<0.05).
  
  
  CONCLUSIONS
  The results of the present study suggested that oral or topical administration of HP extract after myringotomy suppressed the inflammation and fibroblastic activity in the lamina propria of the myringotomized TMs of the rats. Further clinical studies with larger population using HP and other antioxidants will be essential to provide further evidence for use of antioxidant therapy in patients with myringotomy and ventilation tube insertion for otitis media with effusion.
authors.affiliation: Istanbul Medeniyet University, Faculty of Medicine, Department of Otorhinolaryngology, Istanbul, Turkey.
authors.forename: Oğuz Kadir
authors.initials: OK
authors.lastname: Eğilmez
authors.affiliation: Istanbul Medeniyet University, Faculty of Medicine, Department of Otorhinolaryngology, Istanbul, Turkey.
authors.forename: Numan
authors.initials: N
authors.lastname: Kökten
authors.affiliation: Yeditepe University, Faculty of Medicine, Department of Pathology, Istanbul, Turkey.
authors.forename: A Işın Doğan
authors.initials: AI
authors.lastname: Ekici
authors.affiliation: Istanbul Medeniyet University, Faculty of Medicine, Department of Otorhinolaryngology, Istanbul, Turkey. Electronic address: mtkalcioglu@hotmail.com.
authors.forename: M Tayyar
authors.initials: MT
authors.lastname: Kalcıoğlu
authors.affiliation: Yeditepe University, Faculty of Pharmacy, Department of Pharmacognosy, Istanbul, Turkey.
authors.forename: Erdem
authors.initials: E
authors.lastname: Yesilada
authors.affiliation: Istanbul Medeniyet University, Faculty of Medicine, Department of Otorhinolaryngology, Istanbul, Turkey.
authors.forename: Muhammet
authors.initials: M
authors.lastname: Tekin
mesh_terms: D000284:Administration, Oral; D000287:Administration, Topical; D000818:Animals; D005355:Fibrosis / Q000473:pathology / Q000517:prevention & control; D020902:Hypericum; D007249:Inflammation / Q000473:pathology / Q000517:prevention & control; D008297:Male; D023421:Models, Animal; D009092:Mucous Membrane / Q000473:pathology; D063371:Myringosclerosis / Q000517:prevention & control*; D010936:Plant Extracts / Q000008:administration & dosage*; D011183:Postoperative Complications / Q000517:prevention & control*; D017208:Rats, Wistar; D014432:Tympanic Membrane / Q000473:pathology / Q000601:surgery*
chemical_list: D010936:Plant Extracts
keywords: Antiinflammatory; Hypericum perforatum L.; Myringosclerosis; Rat