title: Automated pipeline for purification, biophysical and x-ray analysis of biomacromolecular solutions.
abstract: Small angle X-ray scattering (SAXS), an increasingly popular method for structural analysis of biological macromolecules in solution, is often hampered by inherent sample polydispersity. We developed an all-in-one system combining in-line sample component separation with parallel biophysical and SAXS characterization of the separated components. The system coupled to an automated data analysis pipeline provides a novel tool to study difficult samples at the P12 synchrotron beamline (PETRA-3, EMBL/DESY, Hamburg).
authors.affiliation: European Molecular Biology Laboratory (EMBL) Hamburg, 22607 Hamburg, Germany.
authors.forename: Melissa A
authors.initials: MA
authors.lastname: Graewert
authors.affiliation: European Molecular Biology Laboratory (EMBL) Hamburg, 22607 Hamburg, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Franke
authors.affiliation: European Molecular Biology Laboratory (EMBL) Hamburg, 22607 Hamburg, Germany.
authors.forename: Cy M
authors.initials: CM
authors.lastname: Jeffries
authors.affiliation: European Molecular Biology Laboratory (EMBL) Hamburg, 22607 Hamburg, Germany.
authors.forename: Clement E
authors.initials: CE
authors.lastname: Blanchet
authors.affiliation: European Molecular Biology Laboratory (EMBL) Hamburg, 22607 Hamburg, Germany.
authors.forename: Darja
authors.initials: D
authors.lastname: Ruskule
authors.affiliation: Robert Koch-Institut, Division of Enteropathogenic Bacteria and Legionella (FG11), Burgstr. 37, 38855 Wernigerode, Germany.
authors.forename: Katja
authors.initials: K
authors.lastname: Kuhle
authors.affiliation: Robert Koch-Institut, Division of Enteropathogenic Bacteria and Legionella (FG11), Burgstr. 37, 38855 Wernigerode, Germany.
authors.forename: Antje
authors.initials: A
authors.lastname: Flieger
authors.affiliation: Malvern Instruments GmbH, Rigipsstr. 19, 71083 Herrenberg, Germany.
authors.forename: Bernd
authors.initials: B
authors.lastname: Schäfer
authors.affiliation: Malvern Instruments GmbH, Rigipsstr. 19, 71083 Herrenberg, Germany.
authors.forename: Bernd
authors.initials: B
authors.lastname: Tartsch
authors.affiliation: European Molecular Biology Laboratory (EMBL) Hamburg, 22607 Hamburg, Germany.
authors.forename: Rob
authors.initials: R
authors.lastname: Meijers
authors.affiliation: European Molecular Biology Laboratory (EMBL) Hamburg, 22607 Hamburg, Germany.
authors.forename: Dmitri I
authors.initials: DI
authors.lastname: Svergun
mesh_terms: D001703:Biophysics / Q000379:methods; D046911:Macromolecular Substances / Q000737:chemistry* / Q000302:isolation & purification; D053838:Scattering, Small Angle; D012996:Solutions / Q000737:chemistry*
chemical_list: D046911:Macromolecular Substances; D012996:Solutions