title: Cirrhosis induces apoptosis in renal tissue through intracellular oxidative stress.
abstract: BACKGROUND
  Renal failure is a frequent and serious complication in patients with decompensated cirrhosis.
  
  
  OBJECTIVE
  We aimed to evaluate the renal oxidative stress, cell damage and impaired cell function in animal model of cirrhosis.
  
  
  METHODS
  Secondary biliary cirrhosis was induced in rats by ligation of the common bile duct. We measured TBARS, ROS and mitochondrial membrane potential in kidney as markers of oxidative stress, and activities of the antioxidant enzymes. Relative cell viability was determined by trypan blue dye-exclusion assay. Annexin V-PE was used with a vital dye, 7-AAD, to distinguish apoptotic from necrotic cells and comet assay was used for determined DNA integrity in single cells.
  
  
  RESULTS
  In bile duct ligation animals there was significant increase in the kidney lipoperoxidation and an increase of the level of intracellular ROS. There was too an increase in the activity of all antioxidant enzymes evaluated in the kidney. The percentage viability was above 90% in the control group and in bile duct ligation was 64.66% and the dominant cell death type was apoptosis. DNA damage was observed in the bile duct ligation. There was a decreased in the mitochondrial membrane potential from 71.40% ± 6.35% to 34.48% ± 11.40% in bile duct ligation.
  
  
  CONCLUSIONS
  These results indicate that intracellular increase of ROS cause damage in the DNA and apoptosis getting worse the renal function in cirrhosis.
authors.affiliation: Laboratório de Fisiologia, Universidade Federal de Ciências da Saúde de Porto Alegre - UFCSPA, Porto Alegre, RS, Brasil.
authors.forename: Keli Cristina Simões da
authors.initials: KC
authors.lastname: Silveira
authors.affiliation: Laboratório de Genética Toxicológica, UFCSPA, Porto Alegre, RS, Brasil.
authors.forename: Cassiana Macagnan
authors.initials: CM
authors.lastname: Viau
authors.affiliation: Laboratório de Estresse Oxidativo, Universidade Luterana do Brasil - ULBRA, Canoas, RS, Brasil.
authors.forename: Josiane Raskopf
authors.initials: JR
authors.lastname: Colares
authors.affiliation: Laboratório de Genética Toxicológica, UFCSPA, Porto Alegre, RS, Brasil.
authors.forename: Jenifer
authors.initials: J
authors.lastname: Saffi
authors.affiliation: Laboratório de Estresse Oxidativo, Universidade Luterana do Brasil - ULBRA, Canoas, RS, Brasil.
authors.forename: Norma Possa
authors.initials: NP
authors.lastname: Marroni
authors.affiliation: Laboratório de Fisiologia, Universidade Federal de Ciências da Saúde de Porto Alegre - UFCSPA, Porto Alegre, RS, Brasil.
authors.forename: Marilene
authors.initials: M
authors.lastname: Porawski
mesh_terms: D000818:Animals; D017209:Apoptosis; D004195:Disease Models, Animal; D005434:Flow Cytometry; D007668:Kidney / Q000201:enzymology / Q000473:pathology*; D008106:Liver Cirrhosis, Experimental / Q000201:enzymology / Q000473:pathology*; D008297:Male; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D051437:Renal Insufficiency / Q000201:enzymology / Q000473:pathology*