title: Purification and characterization of a novel β-carotene-9',10'-oxygenase from Saccharomyces cerevisiae ULI3.
abstract: OBJECTIVE
  A novel β-carotene-9,10'-oxygenase (ScBCO2) has been characterized from Saccharomyces cerevisiae ULI3 to convert β-carotene to β-apo-10'-carotenal, which is a precursor of the plant hormone strigolactone.
  
  
  RESULTS
  The ScBCO2 enzyme was purified to homogeneity by ammonium sulfate precipitation, Q sepharose and Superdex-200 chromatography. The molecular mass of the enzyme was ~50 kDa by SDS-PAGE. The purified ScBCO2 enzyme displayed optimal activity at 45 °C and pH 8. Tween 20 (1%, w/v), Trition X-100 (1%, w/v), Mg(2+) (5 mM), Zn(2+) (5 mM), Cu(2+) (5 mM), Ca(2+) (5 mM) or DTT (5 mM) increased in the activity by 3, 7, 14, 17, 23, 26 and 27%, respectively. ScBCO2 only exhibited cleavage activity towards carotenoid substrates containing two β-ionone rings and its catalytic efficiency (kcat/Km) followed the order β-carotene > α-carotene > lutein.
  
  
  CONCLUSIONS
  ScBCO2 could be used as a potential candidate for the enzymatic biotransformation of β-carotene to β-apo-10'-carotenal in biotechnological applications.
authors.affiliation: School of Food and Biological Engineering, Zhengzhou University of Light Industry, 5 Dongfeng Rd, Zhengzhou, 450002, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wei
authors.affiliation: School of Food and Biological Engineering, Zhengzhou University of Light Industry, 5 Dongfeng Rd, Zhengzhou, 450002, People's Republic of China.
authors.forename: Beilei
authors.initials: B
authors.lastname: Jia
authors.affiliation: School of Food and Biological Engineering, Zhengzhou University of Light Industry, 5 Dongfeng Rd, Zhengzhou, 450002, People's Republic of China.
authors.forename: Shen
authors.initials: S
authors.lastname: Huang
authors.affiliation: School of Food and Biological Engineering, Zhengzhou University of Light Industry, 5 Dongfeng Rd, Zhengzhou, 450002, People's Republic of China.
authors.forename: Kunpeng
authors.initials: K
authors.lastname: Yang
authors.affiliation: School of Food and Biological Engineering, Zhengzhou University of Light Industry, 5 Dongfeng Rd, Zhengzhou, 450002, People's Republic of China.
authors.forename: Chunxiao
authors.initials: C
authors.lastname: Jia
authors.affiliation: School of Food and Biological Engineering, Zhengzhou University of Light Industry, 5 Dongfeng Rd, Zhengzhou, 450002, People's Republic of China. duobinmao@126.com.
authors.forename: Duobin
authors.initials: D
authors.lastname: Mao
mesh_terms: D002338:Carotenoids / Q000378:metabolism*; D011232:Chemical Precipitation; D002853:Chromatography, Liquid; D049308:Dioxygenases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D020536:Enzyme Activators / Q000032:analysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007231:Infant, Newborn; D008970:Molecular Weight; D010084:Oxidation-Reduction; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D013379:Substrate Specificity; D013696:Temperature; D019207:beta Carotene / Q000378:metabolism*
chemical_list: D020536:Enzyme Activators; D029701:Saccharomyces cerevisiae Proteins; C427759:beta-apo-10'-carotenal; D019207:beta Carotene; D002338:Carotenoids; C000608135:BCO2 protein, S cerevisiae; D049308:Dioxygenases
keywords: Saccharomyces cerevisiae; Strigolactone; β-Apo-10′-carotenal; β-Carotene; β-Carotene-9,10′-oxygenase