title: Fungal Inositol Pyrophosphate IP7 Is Crucial for Metabolic Adaptation to the Host Environment and Pathogenicity.
abstract: Inositol pyrophosphates (PP-IPs) comprising inositol, phosphate, and pyrophosphate (PP) are essential for multiple functions in eukaryotes. Their role in fungal pathogens has never been addressed. Cryptococcus neoformans is a model pathogenic fungus causing life-threatening meningoencephalitis. We investigate the cryptococcal kinases responsible for the production of PP-IPs (IP7/IP8) and the hierarchy of PP-IP importance in pathogenicity. Using gene deletion and inositol polyphosphate profiling, we identified Kcs1 as the major IP6 kinase (producing IP7) and Asp1 as an IP7 kinase (producing IP8). We show that Kcs1-derived IP7 is the most crucial PP-IP for cryptococcal drug susceptibility and the production of virulence determinants. In particular, Kcs1 kinase activity is essential for cryptococcal infection of mouse lungs, as reduced fungal burdens were observed in the absence of Kcs1 or when Kcs1 was catalytically inactive. Transcriptome and carbon source utilization analysis suggested that compromised growth of the KCS1 deletion strain (Δkcs1 mutant) in the low-glucose environment of the host lung is due to its inability to utilize alternative carbon sources. Despite this metabolic defect, the Δkcs1 mutant established persistent, low-level asymptomatic pulmonary infection but failed to elicit a strong immune response in vivo and in vitro and was not readily phagocytosed by primary or immortalized monocytes. Reduced recognition of the Δkcs1 cells by monocytes correlated with reduced exposure of mannoproteins on the Δkcs1 mutant cell surface. We conclude that IP7 is essential for fungal metabolic adaptation to the host environment, immune recognition, and pathogenicity.
  
  
  OBJECTIVE
  Cryptococcus neoformans is responsible for 1 million cases of AIDS-associated meningitis and ~600,000 deaths annually. Understanding cellular pathways responsible for pathogenicity might have an impact on new drug development. We characterized the inositol polyphosphate kinases Kcs1 and Asp1, which are predicted to catalyze the production of inositol pyrophosphates containing one or two diphosphate moieties (PP-IPs). Using gene deletion analysis and inositol polyphosphate profiling, we confirmed that Kcs1 and Asp1 are major IP6 and IP7 kinases, respectively. Kcs1-derived IP7, but not Asp1-derived IP8, is crucial for pathogenicity. Global expression profiling and carbon source utilization testing suggest that IP7-deficient cryptococci cannot adapt their metabolism to allow growth in the glucose-poor environment of the host lung, and consequently, fungal burdens are significantly reduced. Persistent asymptomatic Δkcs1 mutant infection correlated with decreased mannoprotein exposure on the Δkcs1 mutant surface and reduced phagocytosis. We conclude that IP7 is crucial for the metabolic adaptation of C. neoformans to the host environment and for pathogenicity.
authors.affiliation: Centre for Infectious Diseases and Microbiology, Westmead Millennium Institute, Westmead, and Sydney Medical School-Westmead, University of Sydney, New South Wales, Australia.
authors.forename: Sophie
authors.initials: S
authors.lastname: Lev
authors.affiliation: Centre for Infectious Diseases and Microbiology, Westmead Millennium Institute, Westmead, and Sydney Medical School-Westmead, University of Sydney, New South Wales, Australia.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Li
authors.affiliation: Centre for Infectious Diseases and Microbiology, Westmead Millennium Institute, Westmead, and Sydney Medical School-Westmead, University of Sydney, New South Wales, Australia.
authors.forename: Desmarini
authors.initials: D
authors.lastname: Desmarini
authors.affiliation: Medical Research Council Laboratory for Molecular Cell Biology, University College London, London, United Kingdom.
authors.forename: Adolfo
authors.initials: A
authors.lastname: Saiardi
authors.affiliation: Centre for Immunology and Allergy Research, Westmead Millennium Institute, Westmead, New South Wales, Australia.
authors.forename: Nicole L
authors.initials: NL
authors.lastname: Fewings
authors.affiliation: Centre for Immunology and Allergy Research, Westmead Millennium Institute, Westmead, New South Wales, Australia.
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Schibeci
authors.affiliation: Anatomical Pathology, Westmead Hospital, Westmead and Universities of Sydney and Western Sydney, New South Wales, Australia.
authors.forename: Raghwa
authors.initials: R
authors.lastname: Sharma
authors.forename: Tania C
authors.initials: TC
authors.lastname: Sorrell
authors.affiliation: Centre for Infectious Diseases and Microbiology, Westmead Millennium Institute, Westmead, and Sydney Medical School-Westmead, University of Sydney, New South Wales, Australia julianne.djordjevic@sydney.edu.au.
authors.forename: Julianne T
authors.initials: JT
authors.lastname: Djordjevic
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D003453:Cryptococcosis / Q000382:microbiology / Q000473:pathology; D003455:Cryptococcus neoformans / Q000235:genetics / Q000254:growth & development* / Q000502:physiology*; D011756:Diphosphates / Q000378:metabolism*; D004195:Disease Models, Animal; D017353:Gene Deletion; D007295:Inositol Phosphates / Q000378:metabolism*; D008168:Lung / Q000382:microbiology / Q000473:pathology; D051379:Mice; D017856:Phosphotransferases (Phosphate Group Acceptor) / Q000378:metabolism*; D014774:Virulence; D037521:Virulence Factors / Q000378:metabolism
chemical_list: D011756:Diphosphates; D007295:Inositol Phosphates; D037521:Virulence Factors; D017856:Phosphotransferases (Phosphate Group Acceptor)