title: Dehydrocostus Lactone Inhibits Proliferation, Antiapoptosis, and Invasion of Cervical Cancer Cells Through PI3K/Akt Signaling Pathway.
abstract: OBJECTIVE
  Recent studies found that dehydrocostus lactone (DHC), a traditional Chinese medicine in curing chronic ulcer and inflammation, can inhibit several type of tumor cells. The purpose of this study was to define the role of DHC on cervical cancer cells and to explore its mechanism of action.
  
  
  METHODS
  We used DHC alone or in combination with PI3K/Akt-specific inhibitor LY294002 (LY) to treat Hela cells [human papillomavirus (HPV)-18 positive] and C33a cells (HPV negative). The proliferation, apoptosis, and Akt activation were assessed. Cell invasive ability was assayed in transwell chambers.
  
  
  RESULTS
  We found that DHC significantly inhibited proliferation, antiapoptosis, and invasion of both cells, and reduced the level of p-Akt phosphorylation in these cells, in a dose- or time-dependent manner. In addition, these inhibitions of DHC were significantly strengthened by LY.
  
  
  CONCLUSIONS
  The result suggested that DHC plays a potent role in anticervical cancer in multiple biological aspects through PI3K/Akt signaling pathway, independently of HPV infection. This finding surely adds new knowledge to understand the role of DHC in fighting cancers.
authors.affiliation: Department of Pathology, Cancer Research Institute of Guangdong Medical University, Dongguan, People's Republic of China.
authors.forename: Enping
authors.initials: E
authors.lastname: Jiang
authors.forename: Xiwen
authors.initials: X
authors.lastname: Sun
authors.forename: Haixian
authors.initials: H
authors.lastname: Kang
authors.forename: Liping
authors.initials: L
authors.lastname: Sun
authors.forename: Weifang
authors.initials: W
authors.lastname: An
authors.forename: Yunhong
authors.initials: Y
authors.lastname: Yao
authors.forename: Xinrong
authors.initials: X
authors.lastname: Hu
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D049109:Cell Proliferation / Q000187:drug effects*; D005260:Female; D006801:Humans; D007783:Lactones / Q000494:pharmacology*; D009361:Neoplasm Invasiveness; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D012717:Sesquiterpenes / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*
chemical_list: D007783:Lactones; D012717:Sesquiterpenes; C083030:dehydrocostus lactone; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt