title: Oxidovanadium(IV) sulfate-induced glucose uptake in HepG2 cells through IR/Akt pathway and hydroxyl radicals.
abstract: The insulin-mimetic and anti-diabetic properties of vanadium and related compounds have been well documented both in vitro and in vivo. However, the molecular basis of the link between vanadium and the insulin signaling pathway in diabetes mellitus is not fully described. We investigated the effects of reactive oxygen species (ROS) induced by oxidovanadium(IV) sulfate (VOSO4) on glucose uptake and the insulin signaling pathway in human hepatoma cell line HepG2. Exposure of cells to VOSO4 (5-50 μM) resulted in an increase in glucose uptake, insulin receptor (IR) and protein kinase B (Akt) phosphorylation and intracellular ROS generation. Using Western blot, we found that catalase and sodium formate, but not superoxide dismutase, prevented the increase of hydroxyl radical (·OH) generation and significantly decreased VOSO4-induced IR and Akt phosphorylation. These results suggest that VOSO4-induced ·OH radical, which is a signaling species, promotes glucose uptake via the IR/Akt signaling pathway.
authors.affiliation: Laboratory of Environment and Health, College of Life Sciences, University of Chinese Academy of Sciences, No. 19A YuQuan Road, Beijing 100049, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Laboratory of Environment and Health, College of Life Sciences, University of Chinese Academy of Sciences, No. 19A YuQuan Road, Beijing 100049, China.
authors.forename: Deliang
authors.initials: D
authors.lastname: Chen
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, China.
authors.forename: Pingsheng
authors.initials: P
authors.lastname: Liu
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, China.
authors.forename: Taotao
authors.initials: T
authors.lastname: Wei
authors.affiliation: Laboratory of Environment and Health, College of Life Sciences, University of Chinese Academy of Sciences, No. 19A YuQuan Road, Beijing 100049, China. Electronic address: zhangfang@ucas.ac.cn.
authors.forename: Fang
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Laboratory of Environment and Health, College of Life Sciences, University of Chinese Academy of Sciences, No. 19A YuQuan Road, Beijing 100049, China. Electronic address: dingwj@ucas.ac.cn.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Ding
mesh_terms: D001693:Biological Transport, Active / Q000187:drug effects; D005947:Glucose / Q000378:metabolism*; D056945:Hep G2 Cells; D006801:Humans; D017665:Hydroxyl Radical / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D011972:Receptor, Insulin / Q000378:metabolism*; D015398:Signal Transduction; D017968:Vanadium Compounds / Q000494:pharmacology*
chemical_list: D017968:Vanadium Compounds; D017665:Hydroxyl Radical; C034028:vanadyl sulfate; D011972:Receptor, Insulin; D051057:Proto-Oncogene Proteins c-akt; D005947:Glucose
keywords: Glucose uptake; HepG2 cells; IR/Akt; Oxidovanadium(IV) sulfate; ROS