title: pH triggered in vivo photothermal therapy and fluorescence nanoplatform of cancer based on responsive polymer-indocyanine green integrated reduced graphene oxide.
abstract: We have synthesized a pH-dependent, NIR-sensitive, reduced graphene oxide (rGO) hybrid nano-composite via electrostatic interaction with indocyanine green (ICG) which is designed not only to destroy localized cancer cells but also be minimally invasive to surrounding normal cells. The near-infrared (NIR) irradiated hybrid nano-composites showed pH dependent photo-thermal heat generation capability from pH 5.0 to 7.4 due to the pH response relief and quenching effects of poly(2-dimethyl amino ethyl methacrylate) [poly(PDMAEMA)] with ICG on a single rGO sheet. This pH-triggered relief and quenching mechanism regulated in vitro photo-thermolysis as the pH changed from 5.0 to 7.4. The in vitro cellular uptake and confocal laser scan microscopic (CLSM) images at different pH values show promise for environment sensitive bio-imaging. The NIR-absorbing hybrid nanomaterials showed a remarkably improved in vitro cancer cell targeted photothermal destruction compared to free ICG. Upon local NIR irradiation, these hybrid nano-composites-treated tumors showed necrotic, shrunken, ablation of malignant cells and totally healed after 18 days treatment. Our finding regarding the acidic pH stimulus of cancer cellular environment has proven to be a wining platform for the fight against cancer.
authors.affiliation: Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701, Republic of Korea.
authors.forename: Shazid Md
authors.initials: SM
authors.lastname: Sharker
authors.affiliation: School of Pharmacy, Sungkyunkwan University, 300 Cheoncheon-dong, Jangan-gu, Suwon, Gyeonggi-do 440-746, Republic of Korea.
authors.forename: Jung Eun
authors.initials: JE
authors.lastname: Lee
authors.affiliation: Department of IT Convergence, Korea National University of Transportation, Chungju 380-702, Republic of Korea.
authors.forename: Sung Han
authors.initials: SH
authors.lastname: Kim
authors.affiliation: School of Pharmacy, Sungkyunkwan University, 300 Cheoncheon-dong, Jangan-gu, Suwon, Gyeonggi-do 440-746, Republic of Korea.
authors.forename: Ji Hoon
authors.initials: JH
authors.lastname: Jeong
authors.affiliation: Department of IT Convergence, Korea National University of Transportation, Chungju 380-702, Republic of Korea; Department of Polymer Science and Engineering, Korea National University of Transportation, Chungju 380-702, Republic of Korea.
authors.forename: Insik
authors.initials: I
authors.lastname: In
authors.affiliation: Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701, Republic of Korea. Electronic address: haeshin@kaist.ac.kr.
authors.forename: Haeshin
authors.initials: H
authors.lastname: Lee
authors.affiliation: Department of IT Convergence, Korea National University of Transportation, Chungju 380-702, Republic of Korea; Department of Chemical and Biological Engineering, Korea National University of Transportation, Chungju 380-702, Republic of Korea. Electronic address: parkchem@ut.ac.kr.
authors.forename: Sung Young
authors.initials: SY
authors.lastname: Park
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003692:Delayed-Action Preparations / Q000008:administration & dosage / Q000737:chemistry; D004058:Diffusion; D005456:Fluorescent Dyes / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007208:Indocyanine Green / Q000737:chemistry / Q000627:therapeutic use*; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence / Q000379:methods*; D053769:Nanocapsules / Q000737:chemistry* / Q000648:ultrastructure; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000473:pathology*; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000737:chemistry / Q000627:therapeutic use; D011108:Polymers / Q000737:chemistry; D018511:Systems Integration; D000068936:Theranostic Nanomedicine / Q000379:methods; D016896:Treatment Outcome
chemical_list: D003692:Delayed-Action Preparations; D005456:Fluorescent Dyes; D053769:Nanocapsules; D010087:Oxides; D017319:Photosensitizing Agents; D011108:Polymers; D006108:Graphite; D007208:Indocyanine Green
keywords: Graphene oxide; Indocyanine green; Photothermal therapy; pH dependence