title: Identification of Novel Proteins Co-Purifying with Cockayne Syndrome Group B (CSB) Reveals Potential Roles for CSB in RNA Metabolism and Chromatin Dynamics.
abstract: The CSB protein, a member of the SWI/SNF ATP dependent chromatin remodeling family of proteins, plays a role in a sub-pathway of nucleotide excision repair (NER) known as transcription coupled repair (TCR). CSB is frequently mutated in Cockayne syndrome group B, a segmental progeroid human autosomal recessive disease characterized by growth failure and degeneration of multiple organs. Though initially classified as a DNA repair protein, recent studies have demonstrated that the loss of CSB results in pleiotropic effects. Identification of novel proteins belonging to the CSB interactome may be useful not only for predicting the molecular basis for diverse pathological symptoms of CS-B patients but also for unraveling the functions of CSB in addition to its authentic role in DNA repair. In this study, we performed tandem affinity purification (TAP) technology coupled with mass spectrometry and co-immunoprecipitation studies to identify and characterize the proteins that potentially interact with CSB-TAP. Our approach revealed 33 proteins that were not previously known to interact with CSB. These newly identified proteins indicate potential roles for CSB in RNA metabolism involving repression and activation of transcription process and in the maintenance of chromatin dynamics and integrity.
authors.affiliation: Unit of Molecular Genetics of Aging, Department of Ecology and Biology, University of Tuscia, 01100, Viterbo, Italy.
authors.forename: Serena
authors.initials: S
authors.lastname: Nicolai
authors.affiliation: Unit of Molecular Genetics of Aging, Department of Ecology and Biology, University of Tuscia, 01100, Viterbo, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Filippi
authors.affiliation: Unit of Molecular Genetics of Aging, Department of Ecology and Biology, University of Tuscia, 01100, Viterbo, Italy.
authors.forename: Manuela
authors.initials: M
authors.lastname: Caputo
authors.affiliation: Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Slovak Republic.
authors.forename: Lubos
authors.initials: L
authors.lastname: Cipak
authors.affiliation: Department of Genetics, Comenius University in Bratislava, Slovakia.
authors.forename: Juraj
authors.initials: J
authors.lastname: Gregan
authors.affiliation: Department of Biochemistry, Mass Spectrometry Facility, Max F. Perutz Laboratories, University of Vienna, Vienna, Austria.
authors.forename: Gustav
authors.initials: G
authors.lastname: Ammerer
authors.affiliation: Department of Haematology, University of Cambridge, CB2 0PT, Cambridge, United Kingdom.
authors.forename: Mattia
authors.initials: M
authors.lastname: Frontini
authors.affiliation: Unit of Molecular Genetics of Aging, Department of Ecology and Biology, University of Tuscia, 01100, Viterbo, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Willems
authors.affiliation: Unit of Molecular Genetics of Aging, Department of Ecology and Biology, University of Tuscia, 01100, Viterbo, Italy.
authors.forename: Giorgio
authors.initials: G
authors.lastname: Prantera
authors.affiliation: Center for Radiological Research, Department of Radiation Oncology, Columbia University Medical Center, New York, New York, 10032, United States of America.
authors.forename: Adayabalam S
authors.initials: AS
authors.lastname: Balajee
authors.affiliation: Unit of Molecular Genetics of Aging, Department of Ecology and Biology, University of Tuscia, 01100, Viterbo, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Proietti-De-Santis
mesh_terms: D002460:Cell Line; D002843:Chromatin / Q000235:genetics*; D003057:Cockayne Syndrome / Q000235:genetics*; D004265:DNA Helicases / Q000235:genetics*; D004260:DNA Repair / Q000235:genetics; D045643:DNA Repair Enzymes / Q000235:genetics*; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods; D000075223:Poly-ADP-Ribose Binding Proteins; D012313:RNA / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D002843:Chromatin; D000075223:Poly-ADP-Ribose Binding Proteins; D012313:RNA; D004265:DNA Helicases; C078673:ERCC6 protein, human; D045643:DNA Repair Enzymes