title: Diaphorobacter polyhydroxybutyrativorans sp. nov., a novel poly(3-hydroxybutyrate-co-3-hydroxyvalerate)-degrading bacterium isolated from biofilms.
abstract: A novel Gram-stain-negative, facultatively aerobic and rod-shaped strain, designated SL-205(T), was isolated from the biofilms of a denitrifying reactor using poly(3-hydoxybutyrate-co-3-hydroxyvalerate) as the sole carbon source in Beijing, PR China. A polyphasic taxonomic characterization was performed on the novel isolate. Phylogenetic analyses based on the 16S rRNA gene sequence revealed that strain SL-205(T) is a member of the genus Diaphorobacter. High levels of 16S rRNA gene sequence similarity were found between strain SL-205(T) and Diaphorobacter nitroreducens NA10B(T) (99.4%) and Diaphorobacter oryzae RF3(T) (98.5%), respectively. However, the DNA-DNA relatedness values between strain SL-205(T) and D. nitroreducens NA10B(T) and D. oryzae RF3(T) were 57 ± 1% and 45 ± 1.5%, respectively. The G+C content of the genomic DNA of strain SL-205(T) was 66.8 mol%. The major fatty acids consisted of summed feature 3 (including C16 : 1ω7c and/or iso-C15 : 0 2-OH), C16 : 0 and C18 : 1ω7c. Ubiquinone Q-8 was the only respiratory quinone; the polar lipid profile comprised phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and one uncharacterized phospholipid. We conclude that strain SL-205(T) represents a novel species of the genus Diaphorobacter for which the name Diaphorobacter polyhydroxybutyrativorans is proposed; the type strain is SL-205(T) ( = ACCC 19739(T) = DSM 29460(T)).
authors.affiliation: Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry, Beijing 100097, PR China.|Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Beijing 100097, PR China.
authors.forename: Tianlei
authors.initials: T
authors.lastname: Qiu
authors.affiliation: Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry, Beijing 100097, PR China.|School of Chemical Engineering, Northeast Dianli University, Jilin 132012, PR China.
authors.forename: Zhengyan
authors.initials: Z
authors.lastname: Zuo
authors.affiliation: Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry, Beijing 100097, PR China.
authors.forename: Junlian
authors.initials: J
authors.lastname: Gao
authors.affiliation: Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry, Beijing 100097, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Gao
authors.affiliation: Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry, Beijing 100097, PR China.
authors.forename: Meilin
authors.initials: M
authors.lastname: Han
authors.affiliation: Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry, Beijing 100097, PR China.|School of Chemical Engineering, Northeast Dianli University, Jilin 132012, PR China.
authors.forename: Lijiao
authors.initials: L
authors.lastname: Sun
authors.affiliation: School of Chemical Engineering, Northeast Dianli University, Jilin 132012, PR China.
authors.forename: Lanhe
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry, Beijing 100097, PR China.|Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Beijing 100097, PR China.
authors.forename: Xuming
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D000068476:Beijing; D018441:Biofilms; D019149:Bioreactors / Q000382:microbiology*; D042621:Comamonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008372:Manure; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D011091:Polyesters / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology; D013552:Swine; D014451:Ubiquinone / Q000737:chemistry; D000094122:Polyhydroxybutyrates
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D008372:Manure; D010743:Phospholipids; D011091:Polyesters; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D014451:Ubiquinone; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates; C030778:ubiquinone 8