title: Enhancing Cryptosporidium parvum recovery rates for improved water monitoring.
abstract: Water monitoring is essential to ensure safe drinking water for consumers. However existing methods have several drawbacks, particularly with regard to the poor recovery of Cryptosporidium due to the inability to efficiently elute Cryptosporidium oocysts during the established detection process used by water utilities. Thus the development of new inexpensive materials that could be incorporated into the concentration and release stage that would control Cryptosporidium oocysts adhesion would be beneficial. Here we describe improved filter performance following dip-coating of the filters with a "bioactive" polyacrylate. Specifically 69% more oocysts were eluted from the filter which had been coated with a polymer than on the naked filter alone.
authors.affiliation: Institute of Biological Chemistry, BioPhysics & BioEngineering, School of Engineering and Physical Sciences, Heriot-Watt University, Edinburgh Campus, EH14 1AS Edinburgh, United Kingdom.
authors.forename: Pagona
authors.initials: P
authors.lastname: Pavli
authors.affiliation: School of Chemistry, Joseph Black Building, University of Edinburgh, West Mains Road, EH9 3JJ Edinburgh, United Kingdom.
authors.forename: Sesha
authors.initials: S
authors.lastname: Venkateswaran
authors.affiliation: School of Chemistry, Joseph Black Building, University of Edinburgh, West Mains Road, EH9 3JJ Edinburgh, United Kingdom.
authors.forename: Mark
authors.initials: M
authors.lastname: Bradley
authors.affiliation: Institute of Biological Chemistry, BioPhysics & BioEngineering, School of Engineering and Physical Sciences, Heriot-Watt University, Edinburgh Campus, EH14 1AS Edinburgh, United Kingdom. Electronic address: H.L.Bridle@hw.ac.uk.
authors.forename: Helen
authors.initials: H
authors.lastname: Bridle
mesh_terms: D000818:Animals; D016785:Cryptosporidium parvum / Q000254:growth & development*; D004784:Environmental Monitoring; D005374:Filtration / Q000379:methods; D008567:Membranes, Artificial; D033661:Oocysts; D011108:Polymers / Q000737:chemistry; D012995:Solubility; D012997:Solvents; D014867:Water; D014871:Water Microbiology; D014873:Water Pollutants / Q000032:analysis; D014881:Water Supply
chemical_list: D008567:Membranes, Artificial; D011108:Polymers; D012997:Solvents; D014873:Water Pollutants; D014867:Water
keywords: Cryptosporidiosis outbreaks; Cryptosporidium oocysts; Protozoa; Recovery rate; USEPA 1623; Water monitoring