title: Genetic lineages and antimicrobial resistance genotypes in Staphylococcus aureus from children with atopic dermatitis: detection of clonal complexes CC1, CC97 and CC398.
abstract: The objective was to analyse the genetic lineages of Staphylococcus aureus recovered from nasal and skin samples of atopic dermatitis (AD) paediatric patients, and to characterize the antimicrobial resistance phenotype-genotype and the immune-evasion-cluster (IEC) type of isolates. Forty S. aureus isolates from 35 patients (skin: 26; nasal samples: 14) were characterized. Isolates were submitted to spa-, agr- and multilocus sequence typing. All S. aureus strains analyzed were methicillin-susceptible (MSSA). High genetic diversity was detected among the 40 MSSA isolates (especially among skin isolates), with detection of 27 different spa-types, 20 sequence-types and 16 clonal complexes (CCs). Lineages CC30 and CC5 were predominant among nasal isolates (71% vs 23% skin). Thirteen different CCs were detected among skin isolates, with detection of clades CC1, CC9 and CC398. Antimicrobial resistance rates detected were higher in skin than in nasal isolates, especially for macrolides, aminoglycosides, lincosamides and mupirocin. MSSA strains were characterized into five IEC-types, being A, B and F the predominant ones. MSSA strains of lineages CC45 and CC5 were detected in almost all cases in AD patients with severe Scoring Atopic Dermatitis (SCORAD) and lineages CC8, and CC30 in those with mild or moderate one. As conclusion, high-clonal-diversity was detected among MSSA from AD patients, especially in skin-isolates. Colonization with S. aureus of some CCs seems more associated with AD severity than other lineages.
authors.affiliation: a Area of Biochemistry and Molecular Biology , University of La Rioja , Logroño , Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: Benito
authors.affiliation: b Department of Microbiology , Hospital Royo Villanova , Zaragoza , Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Aspiroz
authors.affiliation: c Department of Dermatology , Hospital San Jorge, Huesca , Spain.|d Aragon Health Sciences Institute , Zaragoza , Spain.
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Gilaberte
authors.affiliation: c Department of Dermatology , Hospital San Jorge, Huesca , Spain.|d Aragon Health Sciences Institute , Zaragoza , Spain.
authors.forename: Rosalía
authors.initials: R
authors.lastname: Sanmartín
authors.affiliation: e Department of Dermatology , Hospital Infantil Universitario del Niño Jesús , Madrid , Spain.
authors.forename: Ángela
authors.initials: Á
authors.lastname: Hernández-Martin
authors.affiliation: f Department of Microbiology , Hospital Infantil del Niño Jesús , Madrid , Spain.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Alonso
authors.affiliation: a Area of Biochemistry and Molecular Biology , University of La Rioja , Logroño , Spain.
authors.forename: Paula
authors.initials: P
authors.lastname: Gómez
authors.affiliation: a Area of Biochemistry and Molecular Biology , University of La Rioja , Logroño , Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Lozano
authors.affiliation: a Area of Biochemistry and Molecular Biology , University of La Rioja , Logroño , Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Torres
mesh_terms: D002648:Child; D003876:Dermatitis, Atopic / Q000382:microbiology*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D013207:Staphylococcal Skin Infections / Q000150:complications / Q000235:genetics* / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000235:genetics*
keywords: Atopic dermatitis; CC1; CC398; CC97; Paediatric patients; S. aureus