title: Multimodal approach to assess tumour vasculature and potential treatment effect with DCE-US and DCE-MRI quantification in CWR22 prostate tumour xenografts.
abstract: The aim of this study was to compare intratumoural heterogeneity and longitudinal changes assessed by dynamic contrast-enhanced ultrasound (DCE-US) and dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) in prostate tumour xenografts. In vivo DCE-US and DCE-MRI were obtained 24 h pre- (day 0) and post- (day 2) radiation treatment with a single dose of 7.5 Gy. Characterization of the tumour vasculature was determined by Brix pharmacokinetic analysis of the time-intensity curves. Histogram analysis of voxels showed significant changes (p < 0.001) from day 0 to day 2 in both modalities for kep , the exchange rate constant from the extracellular extravascular space to the plasma, and kel , the elimination rate constant of the contrast. In addition, kep and kel values from DCE-US were significantly higher than those derived from DCE-MRI at day 0 (p < 0.0001) for both groups. At day 2, kel followed the same tendency for both groups, whereas kep showed this tendency only for the treated group in intermediate-enhancement regions. Regarding kep median values, longitudinal changes were not found for any modality. However, at day 2, kep linked to DCE-US was correlated to MVD in high-enhancement areas for the treated group (p = 0.05). In contrast, correlation to necrosis was detected for the control group in intermediate-enhancement areas (p < 0.1). Intratumoural heterogeneity and longitudinal changes in tumour vasculature were assessed for both modalities. Microvascular parameters derived from DCE-US seem to provide reliable biomarkers during radiotherapy as validated by histology. Furthermore, DCE-US could be a stand-alone or a complementary technique.
authors.affiliation: Department of Physics and Technology, University of Bergen, Bergen, Norway.
authors.forename: N
authors.initials: N
authors.lastname: Arteaga-Marrero
authors.affiliation: Department of Biomedicine, University of Bergen, Bergen, Norway.
authors.forename: C B
authors.initials: CB
authors.lastname: Rygh
authors.affiliation: Department of Clinical Medicine, University of Bergen, Bergen, Norway.
authors.forename: J F
authors.initials: JF
authors.lastname: Mainou-Gomez
authors.affiliation: National Centre for Ultrasound in Gastroenterology, Haukeland University Hospital, Bergen, Norway.
authors.forename: K
authors.initials: K
authors.lastname: Nylund
authors.affiliation: Department of Physics and Technology, University of Bergen, Bergen, Norway.
authors.forename: D
authors.initials: D
authors.lastname: Roehrich
authors.affiliation: Department of Oncology and Medical Physics, Haukeland University Hospital, Bergen, Norway.
authors.forename: J
authors.initials: J
authors.lastname: Heggdal
authors.affiliation: Department of Physics and Technology, University of Bergen, Bergen, Norway.
authors.forename: P
authors.initials: P
authors.lastname: Matulaniec
authors.affiliation: Department of Clinical Medicine, University of Bergen, Bergen, Norway.|National Centre for Ultrasound in Gastroenterology, Haukeland University Hospital, Bergen, Norway.
authors.forename: O H
authors.initials: OH
authors.lastname: Gilja
authors.affiliation: Department of Biomedicine, University of Bergen, Bergen, Norway.|Centre for Cancer Biomarkers (CCBIO), University of Bergen, Norway.
authors.forename: R K
authors.initials: RK
authors.lastname: Reed
authors.affiliation: Section of Immunology, Department of Experimental Medical Science, Lund University, Lund, Sweden.
authors.forename: L
authors.initials: L
authors.lastname: Svensson
authors.affiliation: Division of Dermatology and Venereology, Department of Clinical Sciences, Lund University, Lund, Sweden.
authors.forename: N
authors.initials: N
authors.lastname: Lutay
authors.affiliation: Department of Physics and Technology, University of Bergen, Bergen, Norway.
authors.forename: D R
authors.initials: DR
authors.lastname: Olsen
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis; D045744:Cell Line, Tumor; D003287:Contrast Media / Q000494:pharmacology; D005682:Gadolinium / Q000494:pharmacology; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D051379:Mice; D055806:Microvessels / Q000000981:diagnostic imaging*; D064847:Multimodal Imaging / Q000379:methods*; D009368:Neoplasm Transplantation; D011471:Prostatic Neoplasms / Q000098:blood supply* / Q000000981:diagnostic imaging* / Q000532:radiotherapy; D014183:Transplantation, Heterologous; D047368:Tumor Burden; D014463:Ultrasonography
chemical_list: D014408:Biomarkers, Tumor; D003287:Contrast Media; D005682:Gadolinium
keywords: Brix; CEUS; CWR22; DCE-MRI; DCE-US; cancer; prostate; ultrasound