title: A comparison of linaclotide and lubiprostone dosing regimens on ion transport responses in human colonic mucosa.
abstract: Linaclotide, a synthetic guanylyl cyclase C (GC-C) agonist, and the prostone analog, Lubiprostone, are approved to manage chronic idiopathic constipation and constipation-predominant irritable bowel syndrome. Lubiprostone also protects intestinal mucosal barrier function in ischemia. GC-C signaling regulates local fluid balance and other components of intestinal mucosal homeostasis including epithelial barrier function. The aim of this study was to compare if select dosing regimens differentially affect linaclotide and lubiprostone modulation of ion transport and barrier properties of normal human colonic mucosa. Normal sigmoid colon biopsies from healthy subjects were mounted in Ussing chambers. Tissues were treated with linaclotide, lubiprostone, or vehicle to determine effects on short-circuit current (I sc). Subsequent I sc responses to the cAMP agonist, forskolin, and the calcium agonist, carbachol, were also measured to assess if either drug caused desensitization. Barrier properties were assessed by measuring transepithelial electrical resistance. I sc responses to linaclotide and lubiprostone were significantly higher than vehicle control when administered bilaterally or to the mucosal side only. Single versus cumulative concentrations of linaclotide showed differences in efficacy while cumulative but not single dosing caused desensitization to forskolin. Lubiprostone reduced forskolin responses under all conditions. Linaclotide and lubiprostone exerted a positive effect on TER that was dependent on the dosing regimen. Linaclotide and lubiprostone increase ion transport responses across normal human colon but linaclotide displays increased sensitivity to the dosing regimen used. These findings may have implications for dosing protocols of these agents in patients with constipation.
authors.affiliation: Division of Gastroenterology, Department of Internal Medicine, St. Mary's Hospital, Catholic University of Korea Seoul, Korea ; Division of Gastroenterology, School of Medicine, University of California, San Diego La Jolla, California.
authors.forename: Sang Bum
authors.initials: SB
authors.lastname: Kang
authors.affiliation: Division of Gastroenterology, School of Medicine, University of California, San Diego La Jolla, California.
authors.forename: Ronald R
authors.initials: RR
authors.lastname: Marchelletta
authors.affiliation: Division of Gastroenterology, School of Medicine, University of California, San Diego La Jolla, California.
authors.forename: Harrison
authors.initials: H
authors.lastname: Penrose
authors.affiliation: Division of Gastroenterology, School of Medicine, University of California, San Diego La Jolla, California.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Docherty
authors.affiliation: Division of Biomedical Sciences, School of Medicine, University of California, Riverside Riverside, California.
authors.forename: Declan F
authors.initials: DF
authors.lastname: McCole
keywords: Biopsy; ClC-2; chloride secretion; constipation; epithelial; guanylyl cyclase; irritable bowel syndrome