title: [Research of autophagy activity between rat bone marrow mesenchymal stem neural differentiation].
abstract: OBJECTIVE
  To study the autophagy activity between rat bone marrow stem cells (BMSCs) neural differentiation in order to explore the mechanism involve in this process.
  
  
  METHODS
  BMSCs were passed by 3 generation, then was induced with the revulsant 2% (DMSO) + 200 µmol/L (BHA), NSE expression was detected by immunocytochemical stain, the mRNA expression of autophagy associated genes L3B, Beclinl, Atg5, Atg7, Atg10 were detected by RT-PCR, the autophagy protein LC3B was examined by Western blot and flow cytometry analysis.
  
  
  RESULTS
  BMSCs were passed by 3 generation, the purity of BMSCs could reach more than 90%, the morphology of cells were like fibroblasts, after the revulsant 2% DMSO + 200 µmol/L BRA induced, cells were extended long neurites, like nerve cells, positive rate of NSE staining was (83±5) %, RT-PCR results showed that the expression of autophagy associated genes LC3B, Beclinl, Atg5, Atg7 Atg0 were rised after BMSCs neural differentiation, Western blot analysis showed that the LC3B-II protein expression was increased after neural differentiation and the MFI of L3B was highten by flow cytometry.
  
  
  CONCLUSIONS
  Autophagy is increased after rat BMSC neural differentiation.
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.forename: Chun-tian
authors.initials: CT
authors.lastname: Huang
authors.forename: Cai-fang
authors.initials: CF
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Duan
authors.forename: Xue-fei
authors.initials: XF
authors.lastname: Han
authors.forename: Wen-hai
authors.initials: WH
authors.lastname: Yan
authors.forename: Ying
authors.initials: Y
authors.lastname: Xing
mesh_terms: D000818:Animals; D001343:Autophagy; D002454:Cell Differentiation; D002478:Cells, Cultured; D005434:Flow Cytometry; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009474:Neurons / Q000166:cytology; D051381:Rats