title: Radioprotective effect ofRapana thomasianahemocyanin in gamma induced acute radiation syndrome.
abstract: The radioprotective effect ofRapana thomasianahemocyanin (RtH) against radiation-induced injuries (stomach ulcers, survival time and endogenous haemopoiesis) and post-radiation recovery was investigated in male albino mice (C3H strain). Radiation course was in a dose of 7.5 Gy (LD 100/30 - dose that kills 100% of the mice at 30 days) from137Cs with a dose of 2.05 Gy/min. Radiation injuries were manifested by inducing а hematopoietic form of acute radiation syndrome. RtH was administered intraperitoneally in a single dose of 50, 100, 150 and 200 mg/kg body weight (b. w.) once a day for five consecutive days before irradiation. The results obtained showed that radiation exposure led to (1) 100% mortality rate, (2) ulceration in the stomach mucosa and (3) decrease formation of spleen colonies as a marker of endogenous haemopoiesis. Administration of RtH at a dose of 200 mg/kg provided better protection against radiation-induced stomach ulceration, mitigated the lethal effects of radiation exposure and recovered endogenous haemopoiesis versus irradiated but not supplemented mice. It could be expected that RtH will find a use in mitigating radiation induced injury and enhanced radiorecovery.
authors.affiliation: Scientific Laboratory of Radiation Protection, Radiobiology and Cell Radiobiology, Military Medical Academy , Sofia , Bulgaria.
authors.forename: Ivan
authors.initials: I
authors.lastname: Kindekov
authors.affiliation: The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences , Sofia , Bulgaria.
authors.forename: Milka
authors.initials: M
authors.lastname: Mileva
authors.affiliation: Department of Anatomy and Histology, College of Medicine 'Yordanka Filaretova' , Sofia , Bulgaria.
authors.forename: Dimo
authors.initials: D
authors.lastname: Krastev
authors.affiliation: Scientific Laboratory of Radiation Protection, Radiobiology and Cell Radiobiology, Military Medical Academy , Sofia , Bulgaria.
authors.forename: Vladimira
authors.initials: V
authors.lastname: Vassilieva
authors.affiliation: Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences , Sofia , Bulgaria.
authors.forename: Yuliana
authors.initials: Y
authors.lastname: Raynova
authors.affiliation: The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences , Sofia , Bulgaria.
authors.forename: Lyuba
authors.initials: L
authors.lastname: Doumanova
authors.affiliation: Scientific Laboratory of Radiation Protection, Radiobiology and Cell Radiobiology, Military Medical Academy , Sofia , Bulgaria.
authors.forename: Mitko
authors.initials: M
authors.lastname: Aljakov
authors.affiliation: Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences , Sofia , Bulgaria.
authors.forename: Krassimira
authors.initials: K
authors.lastname: Idakieva
keywords: Rapana thomasiana hemocyanin; acute radiation syndrome; radioprotective effect; spleen colony assay; stomach ulcerations