title: Genome sequence analysis of Ebola virus in clinical samples from three British healthcare workers, August 2014 to March 2015.
abstract: We determined complete viral genome sequences from three British healthcare workers infected with Ebola virus (EBOV) in Sierra Leone, directly from clinical samples. These sequences closely resemble those previously observed in the current Ebola virus disease outbreak in West Africa, with glycoprotein and polymerase genes showing the most sequence variation. Our data indicate that current PCR diagnostic assays remain suitable for detection of EBOV in this epidemic and provide confidence for their continued use in diagnosis.
authors.affiliation: Public Health England, Porton Down, Salisbury, United Kingdom.
authors.forename: A
authors.initials: A
authors.lastname: Bell
authors.forename: K
authors.initials: K
authors.lastname: Lewandowski
authors.forename: R
authors.initials: R
authors.lastname: Myers
authors.forename: D
authors.initials: D
authors.lastname: Wooldridge
authors.forename: E
authors.initials: E
authors.lastname: Aarons
authors.forename: A
authors.initials: A
authors.lastname: Simpson
authors.forename: R
authors.initials: R
authors.lastname: Vipond
authors.forename: M
authors.initials: M
authors.lastname: Jacobs
authors.forename: S
authors.initials: S
authors.lastname: Gharbia
authors.forename: M
authors.initials: M
authors.lastname: Zambon
mesh_terms: D004196:Disease Outbreaks; D029043:Ebolavirus / Q000235:genetics* / Q000302:isolation & purification; D058872:Epidemics; D016679:Genome, Viral / Q000235:genetics*; D006282:Health Personnel; D019142:Hemorrhagic Fever, Ebola / Q000175:diagnosis*; D006801:Humans; D010802:Phylogeny; D017421:Sequence Analysis; D012807:Sierra Leone / Q000453:epidemiology; D014195:Travel