title: Estrogen deficiency inducing shifted cytokines profile in bone marrow stromal cells inhibits Treg cells function in OVX mice.
abstract: The study aims to reveal the effect of estrogen deficiency on Treg cells population in bone marrow in the development of osteoclastogenis with comparing the differences about Treg cells phenotypes and cytokines related with the homeostasis and functions maintenance of Treg cells in bone marrow in OVX mice and health control group. Wide&mdash;type C57BL/6 mice were operated OVX to mimic estrogen deficiency in PMO women. Treg cells population and their surface markers expressions were detected by flow cytometry. Cytokines profiles in bone marrow with examined by real&mdash;time PCR and ELISA analysis. Signal pathways and key modulators responsible to inflammatory cytokines expressions in bone marrow stromal cells were also detected with using western blotting. Estrogen deficiency in OVX mice decreased Treg cells and their functions, and cytokines profile in bone marrow were found shifted in bone marrow when compared with control group. Consistent to these observations, signal pathways in bone marrow stromal cells were reported altered by estrogen deficiency in our model. Estrogen deficiency effects Treg cells population and their functions in OVX mice with altering cytokines profile in bone marrow stromal cells.
authors.affiliation: Jinshan Hospital, Fudan University Department of Orthopaedics Shanghai China.
authors.forename: Q-M
authors.initials: QM
authors.lastname: Zeng
authors.affiliation: Jinshan Hospital, Fudan University Department of Orthopaedics Shanghai China.
authors.forename: D-C
authors.initials: DC
authors.lastname: Liu
authors.affiliation: Jinshan Hospital, Fudan University Department of Orthopaedics Shanghai China.
authors.forename: X-C
authors.initials: XC
authors.lastname: Zhang
authors.affiliation: Jinshan Hospital, Fudan University Department of Orthopaedics Shanghai China.
authors.forename: J-P
authors.initials: JP
authors.lastname: Wu
authors.affiliation: Jinshan Hospital, Fudan University Department of Orthopaedics Shanghai China.
authors.forename: L
authors.initials: L
authors.lastname: Ding
authors.affiliation: Zhongshan Hospital, Fudan University Department of Orthopaedics Shanghai China.
authors.forename: D-H
authors.initials: DH
authors.lastname: Meng
authors.affiliation: Zhongshan Hospital, Fudan University Department of Orthopaedics Shanghai China Zhang.jian&commat;zs&mdash;hospital.sh.cn.
authors.forename: J
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology; D016207:Cytokines / Q000378:metabolism*; D004967:Estrogens / Q000172:deficiency*; D005260:Female; D006801:Humans; D015847:Interleukin-4 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D018655:Lymphocyte Count; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010010:Osteoclasts / Q000166:cytology*; D015663:Osteoporosis, Postmenopausal / Q000503:physiopathology; D010052:Ovariectomy; D015398:Signal Transduction; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D016207:Cytokines; D004967:Estrogens; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4