title: Influence of terpenoids and acarbose on glycosyltransferases produced by strainLeuconostoc mesenteroidesURE 13.
abstract: A study of the influence of different plant terpenoids and amino sugar derivate acarbose on the activity of glycosyltransferase complex and purified dextransucrase fromLeuconostoc mesenteroidesURE 13 strain was carried out. All the tested terpenoids showed an inhibitory effect on glycosyltransferases from strain URE 13 at concentration 0.34 mmol. Out of all studied diterpenoids splendidin showed the strongest inhibitory effect decreasing the activity of both glycosyltransferase complex and dextransucrase with 70% and 90%, respectively. The triterpenoid ursolic showed the second strongest inhibitory effect as the enzyme complex and dextransucrase from strain URE 13 retain 27% and 13% of their initial enzyme activity. Despite the higher degree of inhibition of purified dextransucrase, compared to the enzyme complex, a complete inhibition of the enzyme was not observed at the highest used terpenoid concentration (3.42 mmol). When acarbose was used as an inhibitor, a complete inhibition of dextransucrase was observed at concentration of 6.9 mmol, while the enzyme complex retained 8% of its enzyme activity. Ki values of 0.28 mmol for splendidin, 0.37 mmol for ursolic acid and 0.29 mmol for acarbose were determined from the kinetic studies of purified dextransucrase.
authors.affiliation: Department of Biochemistry and Microbiology, Plovdiv University , Plovdiv , Bulgaria.
authors.forename: Veselin
authors.initials: V
authors.lastname: Bivolarski
authors.affiliation: Department of Biochemistry and Microbiology, Plovdiv University , Plovdiv , Bulgaria.
authors.forename: Tonka
authors.initials: T
authors.lastname: Vasileva
authors.affiliation: Department of Biochemistry and Microbiology, Plovdiv University , Plovdiv , Bulgaria.
authors.forename: Petko
authors.initials: P
authors.lastname: Bozov
authors.affiliation: Department of Biochemistry and Microbiology, Plovdiv University , Plovdiv , Bulgaria.
authors.forename: Ilia
authors.initials: I
authors.lastname: Iliev
keywords: Leuconostoc mesenteroides; acarbose; dextransucrase; inhibition; terpenoids