title: Transcriptome Profiling of the Potato (Solanum tuberosum L.) Plant under Drought Stress and Water-Stimulus Conditions.
abstract: Drought stress can seriously affect tuberization, yield and quality of potato plant. However, the precise molecular mechanisms governing potato stolon's response to drought stress and water supply are not very well understood. In this work, a potato (Solanum tuberosum L.) variant, Ningshu 4, was subjected to severe drought stress treatment (DT) and re-watering treatment (RWT) at tuber bulking stage. Strand-specific cDNA libraries of stolon materials were constructed for paired-end transcriptome sequencing analyses and differentially expressed gene (DEG) examination. In comparison to untreated-control (CT) plants, 3189 and 1797 DEGs were identified in DT and RWT plants and 4154 solely expressed DEGs were screened out from these two comparison groups. Interestingly, 263 genes showed opposite expression patterns in DT and RWT plants. Among them, genes homologous to Protein Phosphatase 2C (PP2C), Aspartic protease in guard cell 1 (ASPG1), auxin-responsive protein, Arabidopsis pseudo response regualtor 2 (APRR2), GA stimulated transcripts in Arabidopsis 6 (GASA6), Calmodulin-like protein 19 (CML19), abscisic acid 8'-hydroxylases and calcium-transporting ATPase, et al. were related with drought-stress and water stimulus response. Sixteen DEGs involved in starch synthesis, accumulation and tuber formation exhibited significantly different expression upon re-watering. In addition, 1630, 1527 and 1596 transcription factor encoding genes were detected in CT, DT and RWT. DEGs of ERF, bHLH, MYB, NAC, WRKY, C2H2, bZIP and HD-ZIP families accounted for 50% in three comparison groups, respectively. Furthermore, characteristics of 565 gene ontology (GO) and 108 Kyoto Encyclopedia of Genes and Genomes pathways (KEGG) were analyzed with the 4154 DEGs. All these results suggest that the drought- and water-stimulus response could be implemented by the regulated expression of metabolic pathway DEGs, and these genes were involved in the endogenous hormone biosynthesis and signal transduction pathways. Our data provide more direct information for future study on the interaction between key genes involved in various metabolic pathways under drought stress in potato.
authors.affiliation: Ningxia Key Laboratory for Agrobiotechnology, Agricultural Bio-Technology Center, Ningxia Academy of Agriculture and Forestry Science, Yinchuan, Ningxia Hui Nationality Autonomous Region, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Gong
authors.affiliation: National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Ningxia Key Laboratory for Agrobiotechnology, Agricultural Bio-Technology Center, Ningxia Academy of Agriculture and Forestry Science, Yinchuan, Ningxia Hui Nationality Autonomous Region, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Gan
authors.affiliation: Ningxia Key Laboratory for Agrobiotechnology, Agricultural Bio-Technology Center, Ningxia Academy of Agriculture and Forestry Science, Yinchuan, Ningxia Hui Nationality Autonomous Region, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Ningxia Key Laboratory for Agrobiotechnology, Agricultural Bio-Technology Center, Ningxia Academy of Agriculture and Forestry Science, Yinchuan, Ningxia Hui Nationality Autonomous Region, China.
authors.forename: Yuchao
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Ningxia Key Laboratory for Agrobiotechnology, Agricultural Bio-Technology Center, Ningxia Academy of Agriculture and Forestry Science, Yinchuan, Ningxia Hui Nationality Autonomous Region, China.
authors.forename: Fengjie
authors.initials: F
authors.lastname: Nie
authors.affiliation: Ningxia Key Laboratory for Agrobiotechnology, Agricultural Bio-Technology Center, Ningxia Academy of Agriculture and Forestry Science, Yinchuan, Ningxia Hui Nationality Autonomous Region, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
authors.affiliation: Ningxia Key Laboratory for Agrobiotechnology, Agricultural Bio-Technology Center, Ningxia Academy of Agriculture and Forestry Science, Yinchuan, Ningxia Hui Nationality Autonomous Region, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Li
authors.affiliation: Guyuan sub-centers of National Potato Improvement Center, Ningxia Academy of Agriculture and Forestry Science, Guyuan, Ningxia Hui Nationality Autonomous Region, China.
authors.forename: Zhiqian
authors.initials: Z
authors.lastname: Guo
authors.affiliation: Guyuan sub-centers of National Potato Improvement Center, Ningxia Academy of Agriculture and Forestry Science, Guyuan, Ningxia Hui Nationality Autonomous Region, China.
authors.forename: Guohui
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Ningxia Key Laboratory for Agrobiotechnology, Agricultural Bio-Technology Center, Ningxia Academy of Agriculture and Forestry Science, Yinchuan, Ningxia Hui Nationality Autonomous Region, China.
authors.forename: Yuxia
authors.initials: Y
authors.lastname: Song
mesh_terms: D055864:Droughts; D020869:Gene Expression Profiling / Q000379:methods*; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D053858:Metabolic Networks and Pathways; D010940:Plant Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods; D011198:Solanum tuberosum / Q000235:genetics / Q000254:growth & development*; D013312:Stress, Physiological; D014867:Water
chemical_list: D010940:Plant Proteins; D014867:Water