title: Ovarian responses of dairy buffalo cows to timed artificial insemination protocol, using new or used progesterone devices, during the breeding season (autumn-winter).
abstract: This study evaluated the effect of new or used P4 devices on the ovarian responses of dairy buffalo that were administered an estradiol (E2) plus progesterone (P4)-based timed artificial insemination (TAI) protocol during the breeding season. On the first day of the TAI protocol, 142 cows were randomly assigned to receive one of the following: a new device (New; 1.0 g of P4; n = 48); a device that had previously been used for 9 days (Used1x, n = 47); or a device that had previously been used for 18 days (Used2x, n = 47). Ultrasound was used to evaluate the following: the presence of a corpus luteum (CL); the diameter of the dominant follicle (ØDF) during protocol; ovulatory response; and pregnancies per AI (P/AI). Despite similar responses among the treatments, there was a significant positive association of the ØDF during TAI protocol with ovulatory responses and number of pregnancies. In conclusion, satisfactory ovarian responses and a satisfactory pregnancy rate were achieved when grazing dairy buffalo were subjected to the TAI protocol in breeding season, independent of whether a new or used P4 device was used. Furthermore, the presence of the larger follicle was associated with a higher ovulation rate and higher P/AI following TAI.
authors.affiliation: Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Sao Paulo, Brazil.
authors.forename: Bruno Moura
authors.identifier: http://orcid.org/0000-0003-4272-9740
authors.initials: BM
authors.lastname: Monteiro
authors.affiliation: Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Sao Paulo, Brazil.|Rural Development Office, Registro, Brazil.
authors.forename: Diego Cavalcante
authors.initials: DC
authors.lastname: de Souza
authors.affiliation: Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Sao Paulo, Brazil.
authors.forename: Guilherme Souza Floriano Machado
authors.initials: GS
authors.lastname: Vasconcellos
authors.affiliation: Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Sao Paulo, Brazil.
authors.forename: Thalita Bueno
authors.initials: TB
authors.lastname: Corrêa
authors.affiliation: National Reference Centre for Hygiene and Technologies of Water Buffalo Farming and Productions, Salerno, Italy.
authors.forename: Domenico
authors.initials: D
authors.lastname: Vecchio
authors.affiliation: Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Sao Paulo, Brazil.
authors.forename: Manoel Francisco
authors.initials: MF
authors.lastname: de Sá Filho
authors.affiliation: Research and Development Unit of Registro, Ribeira Valley, Registro, Brazil.
authors.forename: Nelcio Antonio Tonizza
authors.initials: NA
authors.lastname: de Carvalho
authors.affiliation: Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Sao Paulo, Brazil.
authors.forename: Pietro Sampaio
authors.initials: PS
authors.lastname: Baruselli
mesh_terms: D017738:Alkanesulfonic Acids / Q000008:administration & dosage*; D000818:Animals; D001947:Breeding / Q000379:methods*; D002020:Buffaloes / Q000502:physiology*; D003338:Corpus Luteum / Q000000981:diagnostic imaging; D004958:Estradiol / Q000008:administration & dosage*; D005260:Female; D005466:Fluorocarbons / Q000008:administration & dosage*; D007315:Insemination, Artificial / Q000295:instrumentation / Q000379:methods* / Q000502:physiology*; D006080:Ovarian Follicle / Q000000981:diagnostic imaging; D010053:Ovary / Q000502:physiology*; D010060:Ovulation; D011247:Pregnancy; D012621:Seasons; D014463:Ultrasonography
chemical_list: D017738:Alkanesulfonic Acids; D005466:Fluorocarbons; D004958:Estradiol; C076994:perfluorooctane sulfonic acid
keywords: cyclicity; dairy buffalo; dominant follicle; fixed-time; ovulation