title: Expressions of miR-181a and miR-20a in RPMI8226 cell line and their potential as biomarkers for multiple myeloma.
abstract: Multiple myeloma (MM) is characterized by clonal proliferation of malignant plasma cells in the bone marrow. The anti-tumor activity of bortezomib (a proteosome inhibitor) in MM is challenged by emergence of drug resistance. MicroRNAs (miR) regulate and orchestrate multiple cellular pathways. We investigate the contribution miR-181a and miR-20a expressions' on cell proliferation and apoptosis in RPMI8226 cell line and their influence on bortezomib treatment. RNA isolation, quantitative real-time PCR (qRT-PCR), cell proliferation assay, cell cycle analysis, and cell apoptosis assay were done. Statistical analysis was performed using SPSS 17.0 software (SPSS, Chicago, IL, USA). P values of less than 0.05 were considered statistically significant. RPMI8226 cells seeded in 96-well plates and treated for 24 h with different concentrations of bortezomib showed dose-dependent growth inhibition; expression of both miR-181a and miR-20a were inhibited by bortezomib. We found decrease of miR-181a (60%) and miR-20a (30%) in cells transfected with 20-nM inhibitor. A relative increase of 14-fold in miR-181a and 11-fold in miR-20a was observed in cells transfected with mimics of the same concentration. Transient low expression of miR-181a/20a inhibited proliferation at day 4, and overexpression of miR-181a promoted proliferation. Cells transfected with miR-181a/20a inhibitor within day 4 showed lower survival rate, and low expression of miR-181a on the fourth day after transfection promoted apoptosis. Our findings suggest that miR-181a/20a has a higher expression in MM. miR-181-a expression is proportional to MM tumor burden and could be a biomaker for monitoring treatment. miR-20a shows the potential of a diagnostic biomarker.
authors.affiliation: Medical College of Xi'an Jiaotong University, Xi'an Jiaotong University, Xi'an, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Peng
authors.affiliation: Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital, Medical College of Xi'an Jiaotong University, Xi'an Jiaotong University, Xi'an, People's Republic of China.|Department of Internal Medicine, Life Guard Hospital, Biratnagar, Nepal.
authors.forename: Asmitananda
authors.initials: A
authors.lastname: Thakur
authors.affiliation: Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital, Medical College of Xi'an Jiaotong University, Xi'an Jiaotong University, Xi'an, People's Republic of China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Clinical Laboratory of 521 Hospital of the Ordinance Industry, Zhangba East Street, Xi'an, 710065, People's Republic of China.
authors.forename: Yuanfeng
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital, Medical College of Xi'an Jiaotong University, Xi'an Jiaotong University, 277 Yanta West Street, Xi'an, 710061, Shaanxi Province, People's Republic of China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital, Medical College of Xi'an Jiaotong University, Xi'an Jiaotong University, 277 Yanta West Street, Xi'an, 710061, Shaanxi Province, People's Republic of China.
authors.forename: Ruili
authors.initials: R
authors.lastname: Yuan
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital, Medical College of Xi'an Jiaotong University, Xi'an Jiaotong University, 277 Yanta West Street, Xi'an, 710061, Shaanxi Province, People's Republic of China.
authors.forename: Kaige
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital, Medical College of Xi'an Jiaotong University, Xi'an Jiaotong University, 277 Yanta West Street, Xi'an, 710061, Shaanxi Province, People's Republic of China. guox9512@163.com.
authors.forename: Xuan
authors.initials: X
authors.lastname: Guo
mesh_terms: D017209:Apoptosis / Q000235:genetics; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D009101:Multiple Myeloma / Q000235:genetics* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; C534451:MIRN20a microRNA, human; C540193:MIrn181 microRNA, human; D035683:MicroRNAs
keywords: Apoptosis; Biomarker; Bortezomib; Multiple myeloma; miR-181-a; miR-20a