title: Genes affecting novel seed constituents in Limnanthes alba Benth: transcriptome analysis of developing embryos and a new genetic map of meadowfoam.
abstract: The seed oil of meadowfoam, a new crop in the Limnanthaceae family, is highly enriched in very long chain fatty acids that are desaturated at the Δ5 position. The unusual oil is desirable for cosmetics and innovative industrial applications and the seed meal remaining after oil extraction contains glucolimnanthin, a methoxylated benzylglucosinolate whose degradation products are herbicidal and anti-microbial. Here we describe EST analysis of the developing seed transcriptome that identified major genes involved in biosynthesis and assembly of the seed oil and in glucosinolate metabolic pathways. mRNAs encoding acyl-CoA Δ5 desaturase were notably abundant. The library was searched for simple sequence repeats (SSRs) and single nucleotide polymorphisms (SNPs). Fifty-four new SSR markers and eight candidate gene markers were developed and combined with previously developed SSRs to construct a new genetic map for Limnanthes alba. Mapped genes in the lipid biosynthetic pathway encode 3-ketoacyl-CoA synthase (KCS), Δ5 desaturase (Δ5DS), lysophosphatidylacyl-acyl transferase (LPAT), and acyl-CoA diacylglycerol acyl transferase (DGAT). Mapped genes in glucosinolate biosynthetic and degradation pathways encode CYP79A, myrosinase (TGG), and epithiospecifier modifier protein (ESM). The resources developed in this study will further the domestication and improvement of meadowfoam as an oilseed crop.
authors.affiliation: Department of Crop and Soil Science, Oregon State University , Corvallis, OR , United States of America.
authors.forename: Mary B
authors.initials: MB
authors.lastname: Slabaugh
authors.affiliation: Department of Crop and Soil Science, Oregon State University , Corvallis, OR , United States of America ; Department of Botany and Plant Pathology, Oregon State University , Corvallis OR , United States of America.
authors.forename: Laurel D
authors.initials: LD
authors.lastname: Cooper
authors.affiliation: Monsanto Company , Bengaluru Area , India.
authors.forename: Venkata K
authors.initials: VK
authors.lastname: Kishore
authors.affiliation: Department of Plant Sciences, University of California-Davis , Davis, CA , United States of America.
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Knapp
authors.affiliation: Department of Crop and Soil Science, Oregon State University , Corvallis, OR , United States of America.
authors.forename: Jennifer G
authors.initials: JG
authors.lastname: Kling
keywords: Desaturase; Glucolimnanthin; KCS; LPAT; Limnanthes; Meadowfoam