title: New prenyllipid metabolites identified in Arabidopsis during photo-oxidative stress.
abstract: In the present study, we have identified new prenyllipid metabolites formed during high light stress in Arabidopsis thaliana, whose origin and function remained unknown so far. It was found that plastoquinone-C accumulates mainly in the reduced form under high light conditions, as well as during short-term excess light illumination both in the wild-type and tocopherol biosynthetic vte1 mutant, suggesting that plastoquinone-C, a singlet oxygen-derived prenyllipid, is reduced in chloroplasts by photosystem II or enzymatically, outside thylakoids. Plastoquinone-B, a fatty acid ester of plastoquinone-C, was identified for the first time in Arabidopsis in high light grown wild-type plants and during short-time, excess light illumination of the wild-type plants and the vte1 mutant. The gene expression analysis showed that vte2 gene is most pronouncedly up-regulated among the prenyllipid biosynthetic genes under high light and induction of its expression is mainly caused by an increased level of singlet oxygen, as was demonstrated in experiments with D2 O-treated plants under excess light conditions.
authors.affiliation: Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, 30-387, Poland.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Dłużewska
authors.affiliation: Institute of Plant Physiology, Polish Academy of Sciences, Kraków, 30-239, Poland.
authors.forename: Kamil
authors.initials: K
authors.lastname: Zieliński
authors.affiliation: Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, 30-387, Poland.
authors.forename: Beatrycze
authors.initials: B
authors.lastname: Nowicka
authors.affiliation: Department of Medical Physics and Biophysics, Faculty of Physics and Applied Computer Science, AGH University of Science and Technology, Kraków, 30-059, Poland.
authors.forename: Renata
authors.initials: R
authors.lastname: Szymańska
authors.affiliation: Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, 30-387, Poland.
authors.forename: Jerzy
authors.initials: J
authors.lastname: Kruk
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000235:genetics* / Q000378:metabolism; D017360:Arabidopsis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000528:radiation effects*; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D019751:Intramolecular Transferases / Q000235:genetics* / Q000378:metabolism; D008027:Light; D009154:Mutation; D018384:Oxidative Stress; D045332:Photosystem II Protein Complex / Q000378:metabolism; D010971:Plastoquinone / Q000032:analysis / Q000378:metabolism*; D026082:Singlet Oxygen / Q000378:metabolism*; D020524:Thylakoids / Q000378:metabolism; D024505:Tocopherols / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D045332:Photosystem II Protein Complex; D026082:Singlet Oxygen; D019883:Alkyl and Aryl Transferases; C446531:HPT1 protein, Arabidopsis; D019751:Intramolecular Transferases; C103397:tocopherol cyclase; D010971:Plastoquinone; D024505:Tocopherols
keywords: light stress; plastochromanol; plastoquinone; singlet oxygen; tocopherols