title: Identification of a novel PARP14-TFE3 gene fusion from 10-year-old FFPE tissue by RNA-seq.
abstract: Xp11 (TFE3) translocation renal cell carcinoma (RCC) is officially recognized as a distinct subtype of RCC in the 2004 WHO classification. This neoplasm is characterized by several chromosomal translocations between the TFE3-involving Xp11.2 breakpoint and various fusion partners. To date, five partner genes have been identified, that is, PRCC in 1q21, PSF in 1q34, ASPL in 17q25, CLTC in 17q23, and NONO in Xq12; and three additional translocations have been reported with no partner gene being defined: t(X;3)(p11;q23), t(X;10)(p11;q23), and t(X;19)(p11;q13). Here, we report the identification of a novel TFE3 fusion partner, PARP14 in chromosome band3q21. We used RNA-seq on a 10-year-old FFPE (formalin-fixed, paraffin-embedded) tissue sample, which carried t(X;3)(p11;q23) as detected in the original cytogenetic study. The fusion transcript connected the 5'-end of the first two exons of PARP14 to the 3'-end of five exons of TFE3, which was verified by reverse transcription PCR (RT-PCR) and Sanger sequencing. Similar to other TFE3 fusions previously reported, the predicted PARP14-TFE3 product retains the nuclear localization and DNA-binding domains of TFE3. This finding expands the list of TFE3 translocation partner genes and re-emphasizes the essential oncogenic role of TFE3 fusion proteins in this tumor. Our result also clearly demonstrated the feasibility of identifying chromosomal translocation by RNA-seq in clinical FFPE, which are easily accessible and associated with valuable clinical information. © 2015 Wiley Periodicals, Inc.
authors.affiliation: Department of Pathology, New York Medical College, Westchester Medical Center, Valhalla, NY.
authors.forename: Weihua
authors.initials: W
authors.lastname: Huang
authors.affiliation: Department of Pathology, Hackensack University Medical Center, Hackensack, NJ.
authors.forename: Michael
authors.initials: M
authors.lastname: Goldfischer
authors.affiliation: Department of Pathology, New York Medical College, Westchester Medical Center, Valhalla, NY.
authors.forename: Sabina
authors.initials: S
authors.lastname: Babayeva
authors.affiliation: Clinical Informatics Solutions and Services (CISS), Philips Research North America, Briarcliff Manor, NY.
authors.forename: Yong
authors.initials: Y
authors.lastname: Mao
authors.affiliation: Clinical Informatics Solutions and Services (CISS), Philips Research North America, Briarcliff Manor, NY.
authors.forename: Konstantin
authors.initials: K
authors.lastname: Volyanskyy
authors.affiliation: Clinical Informatics Solutions and Services (CISS), Philips Research North America, Briarcliff Manor, NY.
authors.forename: Nevenka
authors.initials: N
authors.lastname: Dimitrova
authors.affiliation: Department of Pathology, New York Medical College, Westchester Medical Center, Valhalla, NY.
authors.forename: John T
authors.initials: JT
authors.lastname: Fallon
authors.affiliation: Department of Pathology, New York Medical College, Westchester Medical Center, Valhalla, NY.
authors.forename: Minghao
authors.initials: M
authors.lastname: Zhong