title: Cohesion Establishment Factors Stimulate Endonuclease Activity of hFen1 Independently and Cooperatively.
abstract: Human Fen1 protein (hFen1) plays an important role in Okazaki fragment processing by cleaving the flap structure at the junction between single-stranded (ss) DNA and doublestranded (ds) DNA, an intermediate formed during Okazaki fragment processing, resulting in ligatable nicked dsDNA. It was reported that hChlR1, a member of the cohesion establishment factor family, stimulates hFen1 nuclease activity regardless of its ATPase activity. In this study, we found that cohesion establishment factors cooperatively stimulate endonuclease activity of hFen1 in in vivo mimic condition, including replication protein-A-coated DNA and high salt. Our findings are helpful to explain how a DNA replication machinery larger than the cohesion complex goes through the cohesin ring structure on DNA during S phase in the cell cycle.
authors.affiliation: Bio Research Team, LG Household & Health Care Research Park, Daejeon 305-343, Republic of Korea.
authors.forename: Do-Hyung
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Epigenomics Research Center, Korea Research Institute of Bioscience & Biotechnology, Daejeon 305-806, Republic of Korea.
authors.forename: Jeong-Hoon
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Functional Proteomics Research Center, Korea Research Institute of Bioscience & Biotechnology, Daejeon 305-806, Republic of Korea.
authors.forename: Byoung Chul
authors.initials: BC
authors.lastname: Park
authors.affiliation: College of Pharmacy, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Sayeon
authors.initials: S
authors.lastname: Cho
authors.affiliation: Functional Proteomics Research Center, Korea Research Institute of Bioscience & Biotechnology, Daejeon 305-806, Republic of Korea.
authors.forename: Sung Goo
authors.initials: SG
authors.lastname: Park
mesh_terms: D053487:DEAD-box RNA Helicases / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D004265:DNA Helicases / Q000378:metabolism*; D004261:DNA Replication; D045585:Flap Endonucleases / Q000378:metabolism*; D006801:Humans
chemical_list: D004247:DNA; D045585:Flap Endonucleases; C468317:FEN1 protein, human; D004265:DNA Helicases; C101830:DDX11 protein, human; D053487:DEAD-box RNA Helicases
keywords: Fen1; Okazaki fragment processing; cohesion establishment factors