title: The proteome of Hypobaric Induced Hypoxic Lung: Insights from Temporal Proteomic Profiling for Biomarker Discovery.
abstract: Exposure to high altitude induces physiological responses due to hypoxia. Lungs being at the first level to face the alterations in oxygen levels are critical to counter and balance these changes. Studies have been done analysing pulmonary proteome alterations in response to exposure to hypobaric hypoxia. However, such studies have reported the alterations at specific time points and do not reflect the gradual proteomic changes. These studies also identify the various biochemical pathways and responses induced after immediate exposure and the resolution of these effects in challenge to hypobaric hypoxia. In the present study, using 2-DE/MS approach, we attempt to resolve these shortcomings by analysing the proteome alterations in lungs in response to different durations of exposure to hypobaric hypoxia. Our study thus highlights the gradual and dynamic changes in pulmonary proteome following hypobaric hypoxia. For the first time, we also report the possible consideration of SULT1A1, as a biomarker for the diagnosis of high altitude pulmonary edema (HAPE). Higher SULT1A1 levels were observed in rats as well as in humans exposed to high altitude, when compared to sea-level controls. This study can thus form the basis for identifying biomarkers for diagnostic and prognostic purposes in responses to hypobaric hypoxia.
authors.affiliation: Defence Institute of Physiology &Allied Sciences (DIPAS), DRDO, Ministry of Defence, Timarpur, Delhi, India.
authors.forename: Yasmin
authors.initials: Y
authors.lastname: Ahmad
authors.affiliation: Defence Institute of Physiology &Allied Sciences (DIPAS), DRDO, Ministry of Defence, Timarpur, Delhi, India.
authors.forename: Narendra K
authors.initials: NK
authors.lastname: Sharma
authors.affiliation: School of Biotechnology, JNU, New Delhi, India.
authors.forename: Mohammad Faiz
authors.initials: MF
authors.lastname: Ahmad
authors.affiliation: Defence Institute of Physiology &Allied Sciences (DIPAS), DRDO, Ministry of Defence, Timarpur, Delhi, India.
authors.forename: Manish
authors.initials: M
authors.lastname: Sharma
authors.affiliation: Defence Institute of Physiology &Allied Sciences (DIPAS), DRDO, Ministry of Defence, Timarpur, Delhi, India.
authors.forename: Iti
authors.initials: I
authors.lastname: Garg
authors.affiliation: Defence Institute of Physiology &Allied Sciences (DIPAS), DRDO, Ministry of Defence, Timarpur, Delhi, India.
authors.forename: Mousami
authors.initials: M
authors.lastname: Srivastava
authors.affiliation: Defence Institute of Physiology &Allied Sciences (DIPAS), DRDO, Ministry of Defence, Timarpur, Delhi, India.
authors.forename: Kalpana
authors.initials: K
authors.lastname: Bhargava
mesh_terms: D000531:Altitude; D000532:Altitude Sickness / Q000235:genetics* / Q000503:physiopathology; D000818:Animals; D015239:Arylsulfotransferase / Q000096:biosynthesis* / Q000235:genetics; D020869:Gene Expression Profiling; D006801:Humans; D006976:Hypertension, Pulmonary / Q000235:genetics* / Q000503:physiopathology; D000860:Hypoxia / Q000235:genetics* / Q000503:physiopathology; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D020543:Proteome; D040901:Proteomics; D051381:Rats
chemical_list: D020543:Proteome; D015239:Arylsulfotransferase; C573795:Sult1a1 protein, rat