title: In vitro antifungal activity of the essential oil and the methanolic extract of Ruta chalepensis.
abstract: Ruta chalepensis L. (Rutaceae), is an ancient aromatic medicinal plant still used in the traditional medicine of many countries as a laxative, antiinflammatory, analgesic, antispasmodic, abortifacient, antiepileptic, emmenagogue and for dermatopathy treatment. Regarding increasing prevalence of mycotoxins and inefficiency of methods used to decrease them, it is possible to use plants metabolites to decrease mycotoxins. This study was carried out to evaluate chemical composition, antifungal and anticandidal activities of R. chalepensis extracts. The chemical composition of its essential oil was analyzed by gas chromatography coupled with mass spectrometry (GCMS). The major components of R. chalepensis essential oil were menthol (49.92%), linalool (31.1%) and 2-hexanal (5.2%). The antifungal and anticandidal effects of the essential oil and methanolic extract of R. chalepensis leaves were studied by disc diffusion assay and broth dilution method. The obtained results showed that R. chalepensis extracts had a significant fungicidal effect against Aspergillus niger, Aspergillus flavus and Candida albicans. The inhibition zones diameters and the minimum inhibitory concentration values for tested microorganisms were in the range of 11-17 mm and 3.25-6.25% (v/v), respectively. The methanolic extract showed much better antimicrobial activity than the essential oil against three tested micro-organisms
authors.affiliation: Laboratoire d'Epidémiologie et Microbiologie Vétèrinaire, Groupes de Bactériologie et Développement Biotechnologique. Institut Pasteur de Tunis, Université El Manar. chediaaouadhi@yahoo.fr
authors.forename: Ch
authors.initials: Ch
authors.lastname: Aouadhi
authors.forename: H
authors.initials: H
authors.lastname: Ghazghazi
authors.forename: S
authors.initials: S
authors.lastname: Hamrouni
authors.forename: B
authors.initials: B
authors.lastname: Hasnaoui
authors.forename: A
authors.initials: A
authors.lastname: Maaroufi
mesh_terms: D001230:Aspergillus / Q000187:drug effects*; D002176:Candida albicans / Q000187:drug effects*; D000432:Methanol; D009822:Oils, Volatile / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D032105:Ruta
chemical_list: D009822:Oils, Volatile; D010936:Plant Extracts; D000432:Methanol