title: Assessment of the genetic polymorphism and physiological characterization of indigenous Oenococcus oeni strains isolated from Aglianico del Vulture red wine.
abstract: The aim of this study was a reliable intra-species discrimination and strain biodiversity in Oenococcus oeni populations of two different Aglianico wineries by molecular, biochemical, and physiological characterization. Pulsed field gel electrophoresis (PFGE) analysis revealed a high polymorphism related to the origin (winery) of strains, while differential display PCR (DD-PCR) allowed a further discrimination of strains from the same winery. Moreover, the heterogeneity of these natural populations was investigated by capillary electrophoresis and enzymatic assays. A variability related to a different surface charge distribution was observed among strains, linked to their origin. Malolactic activity study evidenced strain-specific differences in malic acid degradation, and then, only the presence of L(-)-malic acid in the medium induced the mle gene. This study provided evidences on the importance of intra-species biodiversity of malolactic bacterial populations in wine ecosystems, as each wine possess peculiar winemaking conditions and physical-chemical properties which make specific the bacterial survival and growth. This study highlighted a great biodiversity among O. oeni strains that can be also winery specific. Such biodiversity within a certain winery and winemaking area is important for selecting malolactic starters, and strain-specific trait identification is especially important to match individual strains to specific industrial process.
authors.affiliation: Dipartimento di Scienze, Università degli Studi della Basilicata, Viale dell'Ateneo Lucano 10, 85100, Potenza, Italy.
authors.forename: Caterina
authors.initials: C
authors.lastname: Cafaro
authors.affiliation: Dipartimento di Scienze, Università degli Studi della Basilicata, Viale dell'Ateneo Lucano 10, 85100, Potenza, Italy. mariagrazia.bonomo@unibas.it.
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Bonomo
authors.affiliation: Dipartimento di Scienze, Università degli Studi della Basilicata, Viale dell'Ateneo Lucano 10, 85100, Potenza, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Guerrieri
authors.affiliation: Dipartimento di Scienze, Università degli Studi della Basilicata, Viale dell'Ateneo Lucano 10, 85100, Potenza, Italy.
authors.forename: Fabiana
authors.initials: F
authors.lastname: Crispo
authors.affiliation: Dipartimento di Scienze, Università degli Studi della Basilicata, Viale dell'Ateneo Lucano 10, 85100, Potenza, Italy.
authors.forename: Rosanna
authors.initials: R
authors.lastname: Ciriello
authors.affiliation: Dipartimento di Scienze, Università degli Studi della Basilicata, Viale dell'Ateneo Lucano 10, 85100, Potenza, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Salzano
mesh_terms: D044822:Biodiversity; D008293:Malates / Q000378:metabolism; D058889:Molecular Typing; D056585:Oenococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D011110:Polymorphism, Genetic; D014920:Wine / Q000382:microbiology*
chemical_list: D008293:Malates; C030298:malic acid