title: Optimization of the Method of Buccal Epithelial Cell Isolation and Culturing on Collagen Substrate for Ophthalmologic Application.
abstract: We performed morphofunctional analysis of cultured rabbit buccal epithelial cells in the primary culture, during passaging, and upon interaction with collagen substrates. Three different morphological types were identified. It was demonstrated that precursors of rabbit buccal epithelium were characterized by high proliferative activity, retain their biological properties throughout long-term culturing, and adhere to various collagen substrates.
authors.affiliation: Helmholtz Moscow Research Institute of Eye Diseases, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Chentsova
authors.forename: O I
authors.initials: OI
authors.lastname: Konyushko
authors.forename: M S
authors.initials: MS
authors.lastname: Makarov
authors.forename: N S
authors.initials: NS
authors.lastname: Egorova
authors.forename: M Yu
authors.initials: MY
authors.lastname: Zinov'ev
authors.forename: N V
authors.initials: NV
authors.lastname: Borovkova
mesh_terms: D000818:Animals; D000867:Anterior Chamber / Q000473:pathology; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D002469:Cell Separation; D064987:Cell- and Tissue-Based Therapy / Q000379:methods*; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology*; D003320:Corneal Ulcer / Q000628:therapy; D004847:Epithelial Cells / Q000166:cytology*; D009061:Mouth Mucosa / Q000166:cytology*; D061251:Primary Cell Culture / Q000379:methods*; D011817:Rabbits
chemical_list: D003094:Collagen