title: Relationship between High-Performance Liquid Chromatography Fingerprints and Uric Acid-Lowering Activities of Cichorium intybus L.
abstract: This study aimed to explore the spectrum-effect relationships between high-performance liquid chromatography fingerprints and the uric acid-lowering activities of chicory. Chemical fingerprints of chicory samples from ten different sources were determined by high-performance liquid chromatography, and then investigated by similarity analysis and hierarchical clustering analysis. Pharmacodynamics experiments were conducted in animals to obtain the uric acid-lowering activity information of each chicory sample. The spectrum-effect relationships between chemical fingerprints and the uric acid-lowering activities of chicory were established by canonical correlation analysis. The structures of potential effective peaks were identified by liquid chromatography with tandem mass spectrometry. The results showed that a close correlation existed between the spectrum and effect of chicory. Aesculin, chlorogenic acid, chicoric acid, isochlorogenic acid A/B/C and 13,14-seco-stigma5(6),14(15)-diene-3α-ol might be the main effective constituents. This work provides a general model of the combination of high-performance liquid chromatography and uric acid-lowering activities to study the spectrum-effect relationships of chicory, which can be used to discover the principle components responsible for the bioactivity.
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. 20130931518@bucm.edu.cn.
authors.forename: Chun-Sheng
authors.initials: CS
authors.lastname: Zhu
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. zhangb@bucm.edu.cn.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhang
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. linzhijian@bucm.edu.cn.
authors.forename: Zhi-Jian
authors.initials: ZJ
authors.lastname: Lin
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. wangxuejie09@sina.com.
authors.forename: Xue-Jie
authors.initials: XJ
authors.lastname: Wang
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. Zhouyuewolf@163.com.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. Sunxiaoxia321@sina.com.
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Sun
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. Shirley0601211@126.com.
authors.forename: Ming-Liang
authors.initials: ML
authors.lastname: Xiao
mesh_terms: D000818:Animals; D002318:Cardiovascular Diseases / Q000097:blood; D018651:Cichorium intybus / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D033461:Hyperuricemia / Q000097:blood / Q000188:drug therapy*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011784:Quail; D014527:Uric Acid / Q000097:blood*
chemical_list: D010936:Plant Extracts; D014527:Uric Acid
keywords: chicory; spectrum-effect relationships; uric acid-lowering