title: Direct observation of tropomyosin binding to actin filaments.
abstract: Tropomyosin is an elongated α-helical coiled coil that binds to seven consecutive actin subunits along the long-pitch helix of actin filaments. Once bound, tropomyosin polymerizes end-to-end and both stabilizes F-actin and regulates access of various actin-binding proteins including myosin in muscle and nonmuscle cells. Single tropomyosin molecules bind weakly to F-actin with millimolar Kd , whereas the end-to-end linked tropomyosin associates with about a 1000-fold greater affinity. Despite years of study, the assembly mechanism of tropomyosin onto actin filaments remains unclear. In this study, we used total internal reflection fluorescence microscopy to directly monitor the cooperative binding of fluorescently labeled tropomyosin molecules to phalloidin-stabilized actin filaments. We find that tropomyosin molecules assemble from multiple growth sites after random low-affinity binding of single molecules to actin. As the length of the tropomyosin chain increases, the probability of detachment decreases, which leads to further chain growth. Tropomyosin chain extension is linearly dependent on the concentration of tropomyosin, occurring at approximately 100 monomers/(μM*s). The random tropomyosin binding to F-actin leads to discontinuous end-to-end association where gaps in the chain continuity smaller than the required seven sequential actin monomers are available. Direct observation of tropomyosin detachment revealed the number of gaps in actin-bound tropomyosin, the time course of gap annealing, and the eventual filament saturation process.
authors.affiliation: Boston University School of Medicine, Physiology, & Biophysics, Boston, Massachusetts.
authors.forename: William M
authors.initials: WM
authors.lastname: Schmidt
authors.affiliation: Boston University School of Medicine, Physiology, & Biophysics, Boston, Massachusetts.
authors.forename: William
authors.initials: W
authors.lastname: Lehman
authors.affiliation: Boston University School of Medicine, Physiology, & Biophysics, Boston, Massachusetts.
authors.forename: Jeffrey R
authors.initials: JR
authors.lastname: Moore
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism*; D000199:Actins / Q000378:metabolism*; D011485:Protein Binding; D014335:Tropomyosin / Q000378:metabolism*
chemical_list: D000199:Actins; D014335:Tropomyosin
keywords: TIRF microscopy; actin-binding proteins; muscle thin filament regulation; single-molecule detection