title: Assessment of cytotoxicity and oxidative stress induced by titanium oxide nanoparticles on Chinook salmon cells.
abstract: Titanium oxide nanoparticles (TiO2 NPs) have received wide attention in diverse application, but the potential impact of these nanomaterials on the environment, aquatic life and especially on fish cell lines is lacking. The present study aimed to investigate the cytotoxicity and oxidative stress induced by TiO2 NPs on Chinook salmon cells derived from Oncorhynchus tshawytscha embryos (CHSE-214). The The MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide] and neutral red (NR) assays in CHSE-214 cells exposed to TiO2 NPs revealed concentration-dependent cytotoxic effect in the range of 10 to 60 μg/ml for 24 h. CHSE-214 cells exposed to TiO2 NPs (10-60 μg/ml) exhibited significant decline in superoxide dismutase (SOD), catalase (CAT) glutathione (GSH) content and increased lipid peroxidation (LPO) in a concentration-dependent manner. TiO2 NPs induced cytotoxicity and oxidative stress in CHSE-214 cells which serve as a base line studies for future studies.
authors.affiliation: CESAM-Centre for Environmental and Marine Studies & Department of Chemistry, University of Aveiro, 3810-193, Aveiro, Portugal. koigooras@ua.pt.|Toxicology Unit, Biology Division, Indian Institute of Chemical Technology, Hyderabad, 500007, India. koigooras@ua.pt.
authors.forename: Koigoora
authors.initials: K
authors.lastname: Srikanth
authors.affiliation: CESAM-Centre for Environmental and Marine Studies & Department of Chemistry, University of Aveiro, 3810-193, Aveiro, Portugal.
authors.forename: Eduarda
authors.initials: E
authors.lastname: Pereira
authors.affiliation: CESAM-Centre for Environmental and Marine Studies & Department of Chemistry, University of Aveiro, 3810-193, Aveiro, Portugal.
authors.forename: Armando C
authors.initials: AC
authors.lastname: Duarte
authors.affiliation: CESAM-Centre for Environmental and Marine Studies & Department of Chemistry, University of Aveiro, 3810-193, Aveiro, Portugal.
authors.forename: Iqbal
authors.initials: I
authors.lastname: Ahmad
authors.affiliation: Toxicology Unit, Biology Division, Indian Institute of Chemical Technology, Hyderabad, 500007, India. jvraoiict@gmail.com.
authors.forename: Janapala Venkateswara
authors.initials: JV
authors.lastname: Rao
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival; D029941:Fish Proteins / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D053768:Metal Nanoparticles / Q000633:toxicity*; D018384:Oxidative Stress; D012474:Salmon / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D014025:Titanium / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D029941:Fish Proteins; D014874:Water Pollutants, Chemical; C009495:titanium dioxide; D014025:Titanium; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione
keywords: Chinook salmon; Cytotoxicity; Lipid peroxidation; Oxidative stress; Titanium oxide nanoparticles