title: Effects of Lu-Do-Huang Extract (LDHE) on Apoptosis Induction in Human Hep3B Cells.
abstract: Lu-Do-Huang (Pracparatum mungo) is a fermented mung bean [corrected] (Vigna radiata) and has long been used as a traditional and functional food in Traditional Chinese Medicine, especially for treating a variety of liver disorders. The present study aimed to evaluate the apoptotic effects of Lu-Do-Huang ethanol extract (LDHE) on Hep3B cells, a human hepatoma cell line. A variety of cellular assays, flow cytometry and immunoblotting were used. Our results showed that LDHE significantly inhibited Hep3B cells growth. Additionally, the cell cycle assay showed that LDHE prevented Hep3B cell entry into S phase and led to an arrest of Hep3B cells in the G₀/G₁ phase. LDHE induced Hep3B cells to undergo apoptosis as determined through Hep3B cell morphology changes, increase of apoptotic bodies, apoptotic cells, DNA fragmentations and caspase activity. We further examined the protein expression of TRADD, FADD, and Bax to verify the possible apoptotic pathways. The results indicated that LDHE-induced apoptosis in Hep3B cells might be mediated [corrected] by an extrinsic signaling pathway leading to an induction of apoptosis in Hep3B cells. In conclusion, LDHE induced apoptosis and cell cycle arrest in Hep3B cells. Our data provide the evidences regarding the anti-hepatoma potential of LDHE in Hep3B cells.
authors.affiliation: Department of Food Science, Nutrition, and Nutraceutical Biotechnology, Shih-Chien University, Taipei 10462, Taiwan, Republic of China.
authors.forename: Hui-Yu
authors.initials: HY
authors.lastname: Huang
authors.affiliation: Department of Basic Sciences and Aquatic Medicine, Norwegian School of Life Sciences, Oslo, Norway.
authors.forename: Li-Han
authors.initials: LH
authors.lastname: Chen
authors.affiliation: Department of Food Science, Nutrition, and Nutraceutical Biotechnology, Shih-Chien University, Taipei 10462, Taiwan, Republic of China.
authors.forename: Chen-Wei
authors.initials: CW
authors.lastname: Liu
authors.affiliation: Department of Food Science, Nutrition, and Nutraceutical Biotechnology, Shih-Chien University, Taipei 10462, Taiwan, Republic of China.
authors.forename: Ting-Yi
authors.initials: TY
authors.lastname: Chien
authors.affiliation: Department of Food Science, Nutrition, and Nutraceutical Biotechnology, Shih-Chien University, Taipei 10462, Taiwan, Republic of China.
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: Yu
authors.affiliation: Department of Food Science, Nutrition, and Nutraceutical Biotechnology, Shih-Chien University, Taipei 10462, Taiwan, Republic of China.
authors.forename: Yu-Yu
authors.initials: YY
authors.lastname: Kao
authors.affiliation: Department of Food Science, Nutrition, and Nutraceutical Biotechnology, Shih-Chien University, Taipei 10462, Taiwan, Republic of China.
authors.forename: Jo-Hsuan
authors.initials: JH
authors.lastname: Yang
authors.affiliation: Institute of Biochemistry and Molecular Biology, National Yang-Ming University, Taipei 11221, Taiwan, Republic of China.
authors.forename: Ying-Chieh
authors.initials: YC
authors.lastname: Tsai
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000502:physiology; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D007887:Fabaceae / Q000737:chemistry; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D010936:Plant Extracts; D020169:Caspases