title: Fabrication and cytocompatibility of in situ crosslinked carbon nanomaterial films.
abstract: Assembly of carbon nanomaterials into two-dimensional (2D) coatings and films that harness their unique physiochemical properties may lead to high impact energy capture/storage, sensors, and biomedical applications. For potential biomedical applications, the suitability of current techniques such as chemical vapor deposition, spray and dip coating, and vacuum filtration, employed to fabricate macroscopic 2D all carbon coatings or films still requires thorough examination. Each of these methods presents challenges with regards to scalability, suitability for a large variety of substrates, mechanical stability of coatings or films, or biocompatibility. Herein we report a coating process that allow for rapid, in situ chemical crosslinking of multi-walled carbon nanotubes (MWCNTs) into macroscopic all carbon coatings. The resultant coatings were found to be continuous, electrically conductive, significantly more robust, and cytocompatible to human adipose derived stem cells. The results lay groundwork for 3D layer-on-layer nanomaterial assemblies (including various forms of graphene) and also opens avenues to further explore the potential of MWCNT films as a novel class of nano-fibrous mats for tissue engineering and regenerative medicine.
authors.affiliation: Department of Biomedical Engineering, Stony Brook University, Stony Brook, NY 11794-5281, USA.
authors.forename: Sunny C
authors.initials: SC
authors.lastname: Patel
authors.affiliation: Department of Biomedical Engineering, Stony Brook University, Stony Brook, NY 11794-5281, USA.
authors.forename: Gaurav
authors.initials: G
authors.lastname: Lalwani
authors.affiliation: Department of Biomedical Engineering, Stony Brook University, Stony Brook, NY 11794-5281, USA.
authors.forename: Kartikey
authors.initials: K
authors.lastname: Grover
authors.affiliation: Department of Biomedical Engineering, Stony Brook University, Stony Brook, NY 11794-5281, USA.
authors.forename: Yi-Xian
authors.initials: YX
authors.lastname: Qin
authors.affiliation: Department of Biomedical Engineering, Stony Brook University, Stony Brook, NY 11794-5281, USA.
authors.forename: Balaji
authors.initials: B
authors.lastname: Sitharaman
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D002244:Carbon / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D006108:Graphite / Q000737:chemistry; D006801:Humans; D018613:Microscopy, Confocal; D049329:Nanostructures / Q000737:chemistry* / Q000633:toxicity; D037742:Nanotubes, Carbon / Q000737:chemistry; D044968:Regenerative Medicine; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D013499:Surface Properties; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials; D037742:Nanotubes, Carbon; D002244:Carbon; D006108:Graphite