title: Storage of washed platelets in BRS-A platelet additive solutions based on two types of clinically available bicarbonated Ringer's solutions with different electrolyte concentrations.
abstract: BACKGROUND
  In Japan, no platelet (PLT) additive solutions (PASs) are officially approved for clinical use although blood centers often receive requests for washed PLTs to reduce adverse reactions. Recently, we developed a novel PAS called BRS-A based on clinically available bicarbonated Ringer's solution (BRS), Bicanate and acid-citrate-dextrose formula A (ACD-A), which has been shown to maintain the in vitro properties of PLTs in the condition of <5% residual plasma during 7-day storage. The aim of this study was to evaluate whether another clinically available BRS, Bicarbon with different electrolyte concentrations can be used as a PAS.
  
  
  METHODS
  Two types of BRS-As were prepared by adding 25 mL of ACD-A to 500 mL of Bicanate or Bicarbon BRSs. Bicanate-based BRS-A and Bicarbon-based BRS-A contain 0.9 or 0.5 mmol/L of magnesium chloride, 95.2 or 100.1 mmol/L of sodium chloride, 4.2 or 5.1 mmol/L of trisodium citrate, and 26.6 or 23.8 mmol/L of sodium bicarbonate, respectively; the other components were identical. Apheresis PLTs stored in these solutions with less than 5% plasma for 7-day storage were compared with regard to their in vitro properties.
  
  
  RESULTS
  The pH levels of all units were above 7 throughout storage. The mean PLT volume, hypotonic shock response, glucose consumption, lactate production, swirling, and CD62P and CD42b expression were similar during 7-day storage. The bicarbonate levels in Bicarbon-based BRS-A were lower than those in Bicanate-based BRS-A.
  
  
  CONCLUSIONS
  Differences in concentrations of electrolytes such as magnesium, sodium, citrate, and bicarbonate salts in BRS-A do not affect the in vitro properties of PLTs during 7-day storage. These results indicate that the use of another type of BRS-A based on Bicarbon as a PAS is feasible. Thus, BRS-A can be used in hospitals that do not stock Bicanate but have Bicarbon.
authors.affiliation: Japanese Red Cross Tohoku Block Blood Center, Sendai, Miyagi, Japan. Electronic address: s-oikawa@th.bbc.jrc.or.jp.
authors.forename: Shinji
authors.initials: S
authors.lastname: Oikawa
authors.affiliation: Japanese Red Cross Tohoku Block Blood Center, Sendai, Miyagi, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Taguchi
authors.affiliation: Japanese Red Cross Tohoku Block Blood Center, Sendai, Miyagi, Japan.
authors.forename: Kimika
authors.initials: K
authors.lastname: Endo
authors.affiliation: Japanese Red Cross Tohoku Block Blood Center, Sendai, Miyagi, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Hoshi
authors.affiliation: Japanese Red Cross Tohoku Block Blood Center, Sendai, Miyagi, Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Kawashima
authors.affiliation: Japanese Red Cross Tohoku Block Blood Center, Sendai, Miyagi, Japan.
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Horibe
authors.affiliation: Japanese Red Cross Tohoku Block Blood Center, Sendai, Miyagi, Japan.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Urano
authors.affiliation: Japanese Red Cross Tohoku Block Blood Center, Sendai, Miyagi, Japan.
authors.forename: Ko
authors.initials: K
authors.lastname: Suzuki
authors.affiliation: Japanese Red Cross Tohoku Block Blood Center, Sendai, Miyagi, Japan.
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Minegishi
authors.affiliation: Japanese Red Cross Tohoku Block Blood Center, Sendai, Miyagi, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Itoh
authors.affiliation: Japanese Red Cross Tohoku Block Blood Center, Sendai, Miyagi, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shimizu
mesh_terms: D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism*; D001793:Blood Preservation / Q000379:methods*; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007552:Isotonic Solutions / Q000737:chemistry* / Q000494:pharmacology*; D008297:Male; D019007:P-Selectin / Q000378:metabolism*; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000378:metabolism*; D000077331:Ringer's Solution; D013997:Time Factors
chemical_list: D007552:Isotonic Solutions; D019007:P-Selectin; D019038:Platelet Glycoprotein GPIb-IX Complex; C546937:SELP protein, human; D000077331:Ringer's Solution
keywords: Bicarbonated Ringer's solution; Electrolyte concentrations; Platelet additive solutions; Platelet washing