title: Purification of glutathione S-transferase from Van Lake fish (Chalcalburnus tarichii Pallas) muscle and investigation of some metal ions effect on enzyme activity.
abstract: Glutathione S-transferases (GSTs) are an important enzyme family which play a critical role in detoxification system. In our study, GST was purified from muscle tissue of Chalcalburnus tarichii Pallas with 301.5-fold purification and 19.07% recovery by glutathione agarose affinity chromatography. The purity of enzyme was checked by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, showing a two band, because of having heterodimer structure. KM values were 1.59 and 0.53 mM for 1-chloro-2,4-dinitrobenzene (CDNB) and glutathione (GSH), respectively. Vmax values for CDNB and GSH were also determined as 5.58 and 1.88 EU/mL, respectively. In addition, inhibition effects of Ag(+), Cu(2+), Cd(2+), Fe(3+), Pb(2+), Cr(2+), Co(2+) and Zn(2+) metal ions were investigated on the enzyme activity and IC50, Ki values were calculated for these metal ions.
authors.affiliation: a Department of Chemistry, Science Faculty , Atatürk University , Erzurum , Turkey.
authors.forename: Mine
authors.initials: M
authors.lastname: Aksoy
authors.affiliation: a Department of Chemistry, Science Faculty , Atatürk University , Erzurum , Turkey.
authors.forename: M Serhat
authors.initials: MS
authors.lastname: Ozaslan
authors.affiliation: a Department of Chemistry, Science Faculty , Atatürk University , Erzurum , Turkey.
authors.forename: O Irfan
authors.initials: OI
authors.lastname: Kufrevioglu
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity; D002847:Chromatography, Agarose; D003530:Cyprinidae; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000737:chemistry; D005982:Glutathione Transferase / Q000037:antagonists & inhibitors* / Q000302:isolation & purification / Q000378:metabolism*; D020128:Inhibitory Concentration 50; D060106:Lakes; D019216:Metals, Heavy / Q000494:pharmacology*; D009132:Muscles / Q000201:enzymology*; D013329:Structure-Activity Relationship
chemical_list: D019216:Metals, Heavy; D005982:Glutathione Transferase; D005978:Glutathione
keywords: Glutathione S-transferase; Van Lake fish; inhibition; metal ions; purification