title: Biodistribution and function of extracellular miRNA-155 in mice.
abstract: Circulating miRNAs can be found in extracellular vesicles (EV) and could be involved in intercellular communication. Here, we report the biodistribution of EV associated miR-155 using miR-155 KO mouse model. Administration of exosomes loaded with synthetic miR-155 mimic into miR-155 KO mice resulted in a rapid accumulation and clearance of miR-155 in the plasma with subsequent distribution in the liver, adipose tissue, lung, muscle and kidney (highest to lowest, respectively). miR-155 expression was detected in isolated hepatocytes and liver mononuclear cells of recipient KO mice suggesting its cellular uptake. In vitro, exosome-mediated restoration of miR-155 in Kupffer cells from miR-155 deficient mice augmented their LPS-induced MCP1 mRNA increase. The systemic delivery of wild type plasma to miR-155 KO mice also resulted in a rapid accumulation of miR-155 in the circulation and distribution to the liver and adipose tissue. In summary, our results demonstrate tissue biodistribution and biologic function of EV-associated miR-155.
authors.affiliation: University of Massachusetts Medical School, Department of Medicine, Worcester, MA 01605.
authors.forename: Shashi
authors.initials: S
authors.lastname: Bala
authors.affiliation: University of Massachusetts Medical School, Department of Medicine, Worcester, MA 01605.
authors.forename: Timea
authors.initials: T
authors.lastname: Csak
authors.affiliation: University of Massachusetts Medical School, Department of Medicine, Worcester, MA 01605.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Momen-Heravi
authors.affiliation: University of Massachusetts Medical School, Department of Medicine, Worcester, MA 01605.
authors.forename: Dora
authors.initials: D
authors.lastname: Lippai
authors.affiliation: University of Massachusetts Medical School, Department of Medicine, Worcester, MA 01605.
authors.forename: Karen
authors.initials: K
authors.lastname: Kodys
authors.affiliation: University of Massachusetts Medical School, Department of Medicine, Worcester, MA 01605.
authors.forename: Donna
authors.initials: D
authors.lastname: Catalano
authors.affiliation: University of Massachusetts Medical School, Department of Medicine, Worcester, MA 01605.
authors.forename: Abhishek
authors.initials: A
authors.lastname: Satishchandran
authors.affiliation: University of Massachusetts Medical School, Department of Molecular Medicine, Worcester, MA 01605.
authors.forename: Victor
authors.initials: V
authors.lastname: Ambros
authors.affiliation: University of Massachusetts Medical School, Department of Medicine, Worcester, MA 01605.
authors.forename: Gyongyi
authors.initials: G
authors.lastname: Szabo
mesh_terms: D000818:Animals; D055354:Exosomes / Q000378:metabolism; D005110:Extracellular Space / Q000378:metabolism; D007728:Kupffer Cells / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D035683:MicroRNAs / Q000097:blood / Q000235:genetics* / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D035683:MicroRNAs; C532623:Mirn155 microRNA, mouse