title: A novel method for separation of Rickettsiales-like organism "Candidatus Xenohaliotis californiensis" from host abalone tissue.
abstract: Filtrations were applied to separate vacuoles of Rickettsiales-like organism Candidatus Xenohaliotis californiensis. Vacuoles were visualized by staining with nucleic acid fluorochrome and their identity was confirmed by Laser Capture Microdissection and PCR. This methodology separates vacuoles and allows studies without the need of isolation in synthetic media or cell lines.
authors.affiliation: Centro de Investigación Científica y Educación Superior de Ensenada, Carretera Ensenada-Tijuana No. 3918, Zona Playitas, 22860 Ensenada, Baja California, Mexico.
authors.forename: Roberto
authors.initials: R
authors.lastname: Cruz-Flores
authors.affiliation: Centro de Investigación Científica y Educación Superior de Ensenada, Carretera Ensenada-Tijuana No. 3918, Zona Playitas, 22860 Ensenada, Baja California, Mexico; Instituto de Sanidad Acuícola, A.C. Calle de la Marina S/N esq. Caracoles, Fraccionamiento Playa Ensenada, Ensenada, Baja California 22880, Mexico. Electronic address: jcaceres@cicese.mx.
authors.forename: Jorge
authors.initials: J
authors.lastname: Cáceres-Martínez
authors.affiliation: Instituto de Sanidad Acuícola, A.C. Calle de la Marina S/N esq. Caracoles, Fraccionamiento Playa Ensenada, Ensenada, Baja California 22880, Mexico.
authors.forename: Rebeca
authors.initials: R
authors.lastname: Vásquez-Yeomans
mesh_terms: D020561:Alphaproteobacteria / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D049851:Gastropoda / Q000382:microbiology*; D061346:Laser Capture Microdissection / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods*; D014617:Vacuoles / Q000737:chemistry
keywords: Abalone; Candidatus Xenohaliotis californiensis; Haliotis; Rickettsiales; Withering syndrome