title: Aerosols how dangerous they are in clinical practice.
abstract: OBJECTIVE
  The purpose of the present study was to determine the microbial atmospheric contamination during initial periodontal treatment using a modern piezoelectric scaler and to evaluate the efficacy of two commercially available mouth rinses (0.2% Chlorhexidine mouth rinse and Listerine) in reducing bacterial contamination when used as a pre-procedural rinse, with and without high volume evacuation (Aerosol reduction device).
  
  
  METHODS
  Subjects for the study were selected from the outpatient Department of Periodontics, Sri Siddhartha Dental College and Hospital, Tumkur, India. Total 60 patients were taken for the study and on the basis of inclusion and exclusion criteria's they were divided into three groups. The sampling was carried out in two stages before and after implementing a set protocol. Total duration of study was four months.
  The samples (blood agar plates) were transported immediately to the Department of Microbiology, Sri Siddhartha Medical College, Tumkur for: Identification of microorganisms as per standard procedures (Gram stain, Biochemical Test, Species Identification).Counting the number of colonies formed on blood agar plates using colony counter unit.
  
  
  RESULTS
  Out of all the three pre-procedural rinses 0.2% w/v Chlorhexidine is the best in reducing aerobic bacteria (CFU) followed by Listerine and then Water.
  
  
  CONCLUSIONS
  The following conclusion was drawn that the use of pre-procedural rinses along with the use of high volume suction apparatus significantly reduced the aerosol contamination and hence chances of cross-infection in the dental units.
authors.affiliation: Assistant Professor, Department of Periodontology and Implantology, Universal College of Medical and Dental Sciences , Bhairahawa, Nepal .
authors.forename: Anshul
authors.initials: A
authors.lastname: Sawhney
authors.affiliation: Professor and HOD, Department of Periodontology and Implantology, Sri Siddhartha Dental College , Tumkur, Karnataka, India .
authors.forename: Sanjay
authors.initials: S
authors.lastname: Venugopal
authors.affiliation: Professor, Department of Microbiology, Sri Siddhartha Dental College , Tumkur, Karnataka, India .
authors.forename: Girish R J
authors.initials: GR
authors.lastname: Babu
authors.affiliation: Senior Lecturer, Department of Pedodontics, Jaipur Dental College , India .
authors.forename: Aarti
authors.initials: A
authors.lastname: Garg
authors.affiliation: Post Graduate Student, Department of Periodontology and Implantology, Sri Siddhartha Dental College , Tumkur, Karnataka, India .
authors.forename: Melwin
authors.initials: M
authors.lastname: Mathew
authors.affiliation: Associate Professor, Department of Periodontology and Implantology, Universal College of Dental Sciences , Bhairahawa Nepal .
authors.forename: Manoj
authors.initials: M
authors.lastname: Yadav
authors.affiliation: Senior Lecturer, Department of Periodontology and Implantology, Mahatma Gandhi Medical College , Mumbai, India .
authors.forename: Bharat
authors.initials: B
authors.lastname: Gupta
authors.affiliation: Assistant Professor, Department of Oral Surgery, Universal College of Dental Sciences , Bhairahawa Nepal .
authors.forename: Shashank
authors.initials: S
authors.lastname: Tripathi
keywords: Piezoelectric scaler; Pre-procedural rinses; Ultrasonic scaling