title: Terminal Deoxynucleotidyl Transferase (TdT)-Mediated dUTP Nick-End Labeling (TUNEL) for Detection of Apoptotic Cells in Drosophila.
abstract: A characteristic feature of apoptosis is DNA fragmentation. This fragmentation can be detected by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL) of DNA in dying cells. Here, we present a protocol for TUNEL detection of apoptosis in Drosophila larval tissue, but these techniques can be adapted for other tissues and developmental stages.
authors.affiliation: Centre for Cancer Biology, University of South Australia, Adelaide, South Australia 5001, Australia.
authors.forename: Donna
authors.initials: D
authors.lastname: Denton
authors.affiliation: Centre for Cancer Biology, University of South Australia, Adelaide, South Australia 5001, Australia.
authors.forename: Sharad
authors.initials: S
authors.lastname: Kumar
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D003856:Deoxyuracil Nucleotides / Q000378:metabolism*; D004330:Drosophila; D020287:In Situ Nick-End Labeling / Q000379:methods*; D007814:Larva / Q000502:physiology*
chemical_list: D003856:Deoxyuracil Nucleotides; C027078:deoxyuridine triphosphate