title: Effect of the pH pre-adjustment in the freezing extender on post-thaw boar sperm quality.
abstract: BACKGROUND
  During freezing the selective precipitation of substances in the medium may provoke a pH shift and lead to sperm damage.
  
  
  OBJECTIVE
  To study the effect of the pH pre-adjustment in the freezing extender on post-thaw boar sperm quality.
  
  
  METHODS
  A total of 15 ejaculates from different boars were obtained and divided into six aliquots prior to a standard straw cryopreservation in freezing extender (lactose-egg yolk-glycerol-Orvus ES Paste) with different pH. After thawing, sperm quality (plasma membrane integrity, motility and acrosome status) were assessed at 30 and 90 minutes of post-thaw incubation at 37 degree C.
  
  
  RESULTS
  When the boar sperm were frozen in a freezing media with pH basic, and particularly at pH 8, it had higher post-thaw sperm quality.
  
  
  CONCLUSIONS
  The pre-adjustment at pH 8 of the freezing extender (lactose-egg yolk-glycerol-Orvus ES Paste) is able to improve the post-thaw boar sperm quality.
authors.affiliation: Centro de Investigacion y Tecnología Animal - Instituto Valenciano de Investigaciones Agrarias(CITA-IVIA), 12400 Segorbe Castellon, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Tomas
authors.affiliation: Centro de Pruebas de Porcino. Area de Investigacion Ganadera, Subdireccion de investigacion y Tecnología. Instituto Tecnologico Agrario. Consejeria de Agricultura y Ganaderia. Junta de Castilla y Leon.Spain.
authors.forename: José
authors.initials: J
authors.lastname: Gómez-Fernandez
authors.affiliation: Centro de Pruebas de Porcino. Area de Investigacion Ganadera, Subdireccion de investigacion y Tecnología. Instituto Tecnologico Agrario. Consejeria de Agricultura y Ganaderia. Junta de Castilla y Leon.Spain.
authors.forename: Emilio
authors.initials: E
authors.lastname: Gómez-Izquierdo
authors.affiliation: Dpto.De Reproducción Animal, INIA, Avda. Puerta de Hierro s/n, 28040 Madrid, Spain.
authors.forename: Ernesto
authors.initials: E
authors.lastname: Gómez-Fidalgo
authors.affiliation: Dpto.De Reproducción Animal, INIA, Avda.Puerta de Hierro s/n, 28040 Madrid, Spain.
authors.forename: Raúl
authors.initials: R
authors.lastname: Sánchez-Sánchez
authors.affiliation: Dpto.De Reproducción Animal, INIA, Avda. Puerta de Hierro s/n, 28040 Madrid, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: González-Bulnes
authors.affiliation: Centro de Pruebas de Porcino. Area de Investigacion Ganadera, Subdireccion de investigacion y Tecnología. Instituto Tecnologico Agrario. Consejeria de Agricultura y Ganaderia. Junta de Castilla y Leon.Spain.
authors.forename: Eduardo
authors.initials: E
authors.lastname: de Mecado
mesh_terms: D000177:Acrosome / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000378:metabolism; D005615:Freezing; D006863:Hydrogen-Ion Concentration; D008297:Male; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D013552:Swine / Q000378:metabolism
chemical_list: D003451:Cryoprotective Agents