title: Effects of ovarian dopaminergic receptors on ovulation.
abstract: Hormonal and neural signals regulate the ovarian follicular development. The present study's hypothesis is that the blockade of ovarian dopamine receptors locally will affect follicle development and ovulation. Groups of adult 4-day cyclic rats of the CII-ZV strain on estrus, diestrus-1, diestrus-2, or proestrus day were injected with vehicle, haloperidol (DA2 > DA1 blocker), sulpiride (DA2 blocker), or SCH-23390 (DA1 blocker) into the bursa of both ovaries at 08:00, 13:00, or 20:00 h. Animals were sacrificed the following predicted estrus day. The following treatments blocked ovulation: injecting haloperidol to rats on estrus or diestrus-1 at 8:00, 13:00, or 20:00 h and to rats on diestrus-2 at 08:00, or 20:00 h; injecting SCH-23390 to rats on diestrus-1 at 8:00, 13:00, or 20:00 h; injecting sulpiride to rats on estrus at 20:00 h, diestrus-1 at 08:00, 13:00, or 20:00 h and to rats on diestrus-2 at 08:00 h. In rats treated with any of the dopamine antagonists that blocked ovulation, injecting GnRH at 14.00 h on the next predicted proestrus day restored ovulation. Injecting estradiol benzoate at 14.00 h of the next predicted diestrus-2 restored ovulation in some animals treated with haloperidol on estrus or diestrus-2 and was ineffective in rats treated on diestrus-1. In rats treated with sulpiride or SCH-23390 ovulation occurred in most animals (SCH-23390: 6/8; SPD: 9/12). Present results suggest that dopamine ovarian receptors' participation in regulating follicular development and ovulation varies along the estrus cycle, with their most prominent activity occurring on diestrus-1.
authors.affiliation: Departamento de Biología y Toxicología de la Reproducción, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, CP.72570, Puebla, Mexico. bvnice@gmail.com.
authors.forename: Berenice
authors.initials: B
authors.lastname: Venegas-Meneses
authors.affiliation: Departamento de Biología y Toxicología de la Reproducción, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, CP.72570, Puebla, Mexico.
authors.forename: José Francisco
authors.initials: JF
authors.lastname: Padilla
authors.affiliation: Departamento de Biología y Toxicología de la Reproducción, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, CP.72570, Puebla, Mexico.
authors.forename: Claudia Elvira
authors.initials: CE
authors.lastname: Juárez
authors.affiliation: Departamento de Biología y Toxicología de la Reproducción, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, CP.72570, Puebla, Mexico.
authors.forename: José Luis
authors.initials: JL
authors.lastname: Morán
authors.affiliation: Departamento de Biología y Toxicología de la Reproducción, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, CP.72570, Puebla, Mexico.
authors.forename: Carolina
authors.initials: C
authors.lastname: Morán
authors.affiliation: Laboratorio de Bioquímica y Biología Molecular, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico.
authors.forename: Nora Hilda
authors.initials: NH
authors.lastname: Rosas-Murrieta
authors.affiliation: Departamento de Biología y Toxicología de la Reproducción, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, CP.72570, Puebla, Mexico.
authors.forename: Anabella
authors.initials: A
authors.lastname: Handal
authors.affiliation: Biology of Reproduction Research Unit, Facultad de Estudios Superiores Zaragoza, UNAM, Mexico, D.F., Mexico.
authors.forename: Roberto
authors.initials: R
authors.lastname: Domínguez
mesh_terms: D000818:Animals; D001552:Benzazepines; D051937:Dopamine and cAMP-Regulated Phosphoprotein 32 / Q000378:metabolism; D004958:Estradiol / Q000031:analogs & derivatives / Q000097:blood*; D030762:Estrous Cycle; D005260:Female; D007987:Gonadotropin-Releasing Hormone; D006220:Haloperidol; D006080:Ovarian Follicle / Q000502:physiology*; D010060:Ovulation; D011374:Progesterone / Q000097:blood*; D011897:Random Allocation; D051381:Rats; D011954:Receptors, Dopamine / Q000502:physiology*; D013469:Sulpiride; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D001552:Benzazepines; D051937:Dopamine and cAMP-Regulated Phosphoprotein 32; C497930:Ppp1r1b protein, rat; D011954:Receptors, Dopamine; C534628:SCH 23390; C074283:estradiol 3-benzoate; D007987:Gonadotropin-Releasing Hormone; D011374:Progesterone; D004958:Estradiol; D013469:Sulpiride; D014446:Tyrosine 3-Monooxygenase; D006220:Haloperidol
keywords: Dopamine receptors; Estrus cycle; Hormone replacement; Ovulation