title: Production of a new non-specific nuclease fromYersinia enterocoliticasubsp.palearctica: optimization of induction conditions using response surface methodology.
abstract: A new non-specific nuclease fromYersinia enterocoliticasubsp.palearctica(Y. NSN) was expressed inEscherichia coli(E. coli) BL 21 StarTM(DE3)plysS. Induction conditions, including isopropyl-β-D-thiogalactoside (IPTG) concentration, cell density (OD600), induction time and induction temperature, were optimized using response surface methodology. Statistical analysis of the results revealed that induction temperature and all the quadratic terms of variables had significant effects on enzyme activity ofY. NSN. The optimal induction conditions were as follows: 1.5 mmol/L IPTG, OD600 of 0.80, induction time of 20.5 h, and induction temperature of 32 °C. Under the optimized conditions, the highest enzyme activity could be obtained.
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University , Hangzhou , Zhejiang , P.R. China.
authors.forename: Xiu-Juan
authors.initials: XJ
authors.lastname: Fang
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University , Hangzhou , Zhejiang , P.R. China ; Date Palm Research Center, King Faisal University , Al-hasa , Saudi Arabia ; Department of Food Science, Anqing Vocational & Technical College , Anqing , Anhui , P.R. China.
authors.forename: Zhen-Xing
authors.initials: ZX
authors.lastname: Tang
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University , Hangzhou , Zhejiang , P.R. China.
authors.forename: Zhen-Hua
authors.initials: ZH
authors.lastname: Li
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University , Hangzhou , Zhejiang , P.R. China.
authors.forename: Zhi-Liang
authors.initials: ZL
authors.lastname: Zhang
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University , Hangzhou , Zhejiang , P.R. China.
authors.forename: Lu-E
authors.initials: LE
authors.lastname: Shi
keywords: Y. enterocolitica subsp. palearctica; induction conditions; non-specific nuclease; response surface methodology