title: [Comparative study of four alkaloids contents and antitussive activities of Stemona tuberosa from different habitats of Guangxi Province].
abstract: OBJECTIVE
  To compare the contents of four alkaloids and antitussive activities of Stemona tuberosa from different habitats of Guangxi Province.
  
  
  METHODS
  The HPLC separation was performed on a Merck Purospher STAR RP18 (250 mm x 4. 6 mm, 5 µm) column by gradient elution using 0. 05% ammonia-acetonitrile as the mobile phase. The flow rate was 1. 0 mL/min, the dectection wave-length was set at 210 nm,and the column temperature was 40 °C. The antitussive potency of total alkaloids of Stemonae Radix from different habitats was evaluated on guinea pigs with citric acid aerosol to induce cough.
  
  
  RESULTS
  The range of recoveries of this mehtod was 98. 24% ~ 101. 21%, with all the constituents showing good linearity(the correlation coefficents above 0. 999). The major chemotype of Stemonae Radix in Guangxi was stemoninine, following by tuberostemonine and croomine, and finally neotuberostemonine. The antitussive activitiy of Stemona tuberosa was in a concentration-dependent manner.
  
  
  CONCLUSIONS
  Stemonae Radix from Dongxing, Fangcheng can reduce cough times and prolong cough incubation period, and thus Dongxing, Fangcheng is the best habitat in Guangxi in the present experiments.
authors.forename: Jing-ni
authors.initials: JN
authors.lastname: Liao
authors.forename: Shan-ding
authors.initials: SD
authors.lastname: Qin
authors.forename: Xiao-sheng
authors.initials: XS
authors.lastname: Qu
authors.forename: Kui
authors.initials: K
authors.lastname: Lin
authors.forename: Dao-Ping
authors.initials: DP
authors.lastname: Huang
authors.forename: Feng-xin
authors.initials: FX
authors.lastname: Li
authors.forename: Lan-lan
authors.initials: LL
authors.lastname: Fan
mesh_terms: D000470:Alkaloids / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D000996:Antitussive Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002681:China; D002851:Chromatography, High Pressure Liquid; D003371:Cough / Q000188:drug therapy; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D017753:Ecosystem; D006168:Guinea Pigs; D006575:Heterocyclic Compounds, 3-Ring; D007783:Lactones; D018517:Plant Roots / Q000737:chemistry; D011759:Pyrrolidines; D013141:Spiro Compounds; D041901:Stemonaceae / Q000737:chemistry*
chemical_list: D000470:Alkaloids; D000996:Antitussive Agents; D004365:Drugs, Chinese Herbal; D006575:Heterocyclic Compounds, 3-Ring; D007783:Lactones; D011759:Pyrrolidines; D013141:Spiro Compounds; C468415:neotuberostemonine; C559140:stemoninine; C034243:tuberostemonine; C082845:croomine