title: [Compound K suppresses myeloid-derived suppressor cells in a mouse model bearing CT26 colorectal cancer xenograft].
abstract: OBJECTIVE
  To investigate the effect of ginseng-derived compound K (C-K) on apoptosis, immunosuppressive activity, and pro-inflammatory cytokine production of myeloid-derived suppressor cells (MDSCs) from mice bearing colorectal cancer xenograft.
  
  
  METHODS
  Flow-sorted bone marrow MDSCs from Balb/c mice bearing CT26 tumor xenograft were treated with either C-K or PBS for 96 h and examined for apoptosis with Annexin V/7-AAD, Cox-2 and Arg-1 expressions using qRT-PCR, and supernatant IL-1β, IL-6, and IL-17 levels with ELISA. C-K- or PBS-treated MDSCs were subcutaneously implanted along with CT26 tumor cells in WT Balb/c mice, and the tumor size and morphology were evaluated 21 days later.
  
  
  RESULTS
  C-K treatment significantly increased the percentages of early and late apoptotic MDSCs in vitro (P<0.01 and P<0.05, respectively), decreased the expressions of immunosuppression-related genes Cox-2 (P<0.05) and Arg-1 (P<0.01), and suppressed the production of IL-1β (P<0.05), IL-6 (P<0.01), and IL-17 (P<0.05) by the MDSCs . Compared with PBS-pre-treated cells, C-K-pretreated MDSCs showed significantly attenuated activity in promoting CT26 tumor growth in mice (P<0.01).
  
  
  CONCLUSIONS
  C-K can suppress the immunosuppresive effect of MDSCs to inhibit tumor cell proliferation in mice, which suggests a new strategy of tumor therapy by targeting MDSCs.
authors.affiliation: Compound K suppresses myeloid-derived suppressor cells in a mouse model bearing CT26 colorectal cancer xenograft.E-mail: 1135014577@qq.com.
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
authors.forename: Yalin
authors.initials: Y
authors.lastname: Li
authors.forename: Wuzhou
authors.initials: W
authors.lastname: Wang
authors.forename: Meijuan
authors.initials: M
authors.lastname: Zhou
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Cao
mesh_terms: D000818:Animals; D017209:Apoptosis; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000473:pathology*; D004195:Disease Models, Animal; D036145:Ginsenosides / Q000494:pharmacology*; D006801:Humans; D007165:Immunosuppression Therapy; D020381:Interleukin-17 / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D022423:Myeloid Cells / Q000187:drug effects*; D009368:Neoplasm Transplantation
chemical_list: D036145:Ginsenosides; D020381:Interleukin-17; D053583:Interleukin-1beta; D015850:Interleukin-6; C112772:ginsenoside compound K