title: MicroRNA-27a-3p Inhibits Melanogenesis in Mouse Skin Melanocytes by Targeting Wnt3a.
abstract: MicroRNAs (miRNAs) play an essential role in the regulation of almost all the biological processes, including melanogenesis. MiR-27a-3p is nearly six times higher in white alpaca skin compared to brown skin, which indicates that miR-27a-3p may be a candidate regulator for melanogenesis. Wnt3a plays an important role in promoting melanoblasts to differentiate into melanocytes and melanogenesis. To confirm the function of miR-27a-3p to melanogenesis in mammals, miR-27a-3p mimic, inhibitor and their negative control were transfected into mouse melanocytes. As a result, miR-27a-3p inhibits melanogenesis by repressing Wnt3a at post-transcriptional level. A significant decrease in Wnt3a luciferase activity was observed in 293T cells co-transfected with the matched luciferase reporter vector and pre-miR-27a. Furthermore, the presence of exogenous miR-27a-3p significantly decreased Wnt3a protein expression rather than mRNA and reduced β-catenin mRNA levels in melanocytes. The over-expression of miR-27a-3p significantly increased the melanin content of melanocytes. However, miR-27a-3p inhibitor performs an opposite effect on melanogenesis. Wnt3a is one target of miR-27a-3p. MiR-27a-3p could inhibit Wnt3a protein amount by post-transcriptional regulation and melanogenesis in mouse melanocytes. Previous studies reported that Wnt3a promoted melanogenensis in mouse melanocytes. Thus, miR-27-3p inhibits melanogenesis by repressing Wnt3a protein expression.
authors.affiliation: College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China. 84840293@163.com.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China. wangsh0402@163.com.
authors.forename: Pengchao
authors.initials: P
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China. mjz122021@126.com.
authors.forename: Jinzhu
authors.initials: J
authors.lastname: Meng
authors.affiliation: College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China. codecass@163.com.
authors.forename: Yuankai
authors.initials: Y
authors.lastname: Ji
authors.affiliation: College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China. carrol-xv@sohu.com.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Xu
authors.affiliation: College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China. chentianzhi15@163.com.
authors.forename: Tianzhi
authors.initials: T
authors.lastname: Chen
authors.affiliation: College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China. ruiwenfan@163.com.
authors.forename: Ruiwen
authors.initials: R
authors.lastname: Fan
authors.affiliation: College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China. yxjfkh@126.com.
authors.forename: Xiuju
authors.initials: X
authors.lastname: Yu
authors.affiliation: College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China. Jianbo.Yao@mail.wvu.edu.|Laboratory of Animal Biotechnology and Genomics, Division of Animal and Nutritional Sciences, West Virginia University, Morgantown, WV 26506, USA. Jianbo.Yao@mail.wvu.edu.
authors.forename: Jianbo
authors.initials: J
authors.lastname: Yao
authors.affiliation: College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China. cs_dong@sxau.edu.cn.
authors.forename: Changsheng
authors.initials: C
authors.lastname: Dong
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D008543:Melanins / Q000378:metabolism*; D008544:Melanocytes / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D060509:Wnt3A Protein / Q000235:genetics* / Q000378:metabolism; D051176:beta Catenin / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; D008543:Melanins; D035683:MicroRNAs; C540674:Mirn27 microRNA, mouse; D012333:RNA, Messenger; D060509:Wnt3A Protein; C555511:Wnt3a protein, mouse; D051176:beta Catenin
keywords: Wnt3a; melanogenesis; miR-27a-3p; post-transcriptional regulation