title: Comparative proteomic analysis using 2DE-LC-MS/MS reveals the mechanism of Fuzhuan brick tea extract against hepatic fat accumulation in rats with nonalcoholic fatty liver disease.
abstract: Fuzhuan brick tea has received increasing attention in recent years owing to its benefits for nonalcoholic fatty liver disease (NAFLD) and associated metabolic syndrome. For exploring the ameliorative mechanism, the liver proteomes from three groups of rats fed either a normal control diet (NCD), a high fat diet (HFD), or a HFD supplemented with high-dose Fuzhuan brick tea extract (FTE) (HFD + HFTE) were comprehensively compared by quantitative proteomics using 2DE-LC-MS/MS. This is the first study of the effects of tea aqueous extract on the liver proteome of rats suffering from metabolic syndrome. The results showed that 57 proteins displayed more than 1.5-fold differences in at least one of two comparisons of HFD versus NCD and HFD versus HFD + HFTE due to HFD feeding and FTE treatment, respectively. Of them, over 75% of proteins exhibited a similar tendency of expression in the two comparisons, meaning FTE was able to correct HFD effects on rat livers. By function analyses, an extensive list of proteins was involved in sugar and lipid metabolism. Compared with HFD-fed rats, the reduced lipogenesis and enhanced β-oxidation, tricarboxylic acid cycle and respiratory chain in HFD + HFTE-fed rats, which mainly contributed to ameliorate hepatic fat accumulation and associated NAFLD. Additionally, some putative drug targets were also revealed such as COX2, PGAM1, ACACB, FAS, and ECHS1.
authors.affiliation: Department of Chemistry of Tsinghua University and Key Laboratory of Biological Organic Phosphorus and Chemical Biology of Ministry of Education, Beijing, P. R. China.|National Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, P. R. China.|Key Laboratory of Tea Science of Ministry of Education, College of Horticulture and Landscape, Hunan Agricultural University, Changsha, P. R. China.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Liu
authors.affiliation: National Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, P. R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Lin
authors.affiliation: National Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, P. R. China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Tea Science of Ministry of Education, College of Horticulture and Landscape, Hunan Agricultural University, Changsha, P. R. China.
authors.forename: Die
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Chemistry of Tsinghua University and Key Laboratory of Biological Organic Phosphorus and Chemical Biology of Ministry of Education, Beijing, P. R. China.
authors.forename: Qionglin
authors.initials: Q
authors.lastname: Liang
authors.affiliation: Department of Chemistry of Tsinghua University and Key Laboratory of Biological Organic Phosphorus and Chemical Biology of Ministry of Education, Beijing, P. R. China.
authors.forename: Guoan
authors.initials: G
authors.lastname: Luo
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D015870:Gene Expression / Q000187:drug effects; D008297:Male; D065626:Non-alcoholic Fatty Liver Disease / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D011506:Proteins / Q000032:analysis / Q000378:metabolism; D020543:Proteome / Q000032:analysis / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry; D013662:Tea / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D020011:Protective Agents; D011506:Proteins; D020543:Proteome; D013662:Tea
keywords: Ameliorative mechanism; Comparative proteomics; Fat accumulation; Fuzhuan brick tea extract (FTE); Nonalcoholic fatty liver disease