title: Metabolic profiling reveals ethylene mediated metabolic changes and a coordinated adaptive mechanism of 'Jonagold' apple to low oxygen stress.
abstract: Apples are predominantly stored in controlled atmosphere (CA) storage to delay ripening and prolong their storage life. Profiling the dynamics of metabolic changes during ripening and CA storage is vital for understanding the governing molecular mechanism. In this study, the dynamics of the primary metabolism of 'Jonagold' apples during ripening in regular air (RA) storage and initiation of CA storage was profiled. 1-Methylcyclopropene (1-MCP) was exploited to block ethylene receptors and to get insight into ethylene mediated metabolic changes during ripening of the fruit and in response to hypoxic stress. Metabolic changes were quantified in glycolysis, the tricarboxylic acid (TCA) cycle, the Yang cycle and synthesis of the main amino acids branching from these metabolic pathways. Partial least square discriminant analysis of the metabolic profiles of 1-MCP treated and control apples revealed a metabolic divergence in ethylene, organic acid, sugar and amino acid metabolism. During RA storage at 18°C, most amino acids were higher in 1-MCP treated apples, whereas 1-aminocyclopropane-1-carboxylic acid (ACC) was higher in the control apples. The initial response of the fruit to CA initiation was accompanied by an increase of alanine, succinate and glutamate, but a decline in aspartate. Furthermore, alanine and succinate accumulated to higher levels in control apples than 1-MCP treated apples. The observed metabolic changes in these interlinked metabolites may indicate a coordinated adaptive strategy to maximize energy production.
authors.affiliation: Department of Biosystems (BIOSYST), Division of Mechatronics, Biostatistics and Sensors (MeBioS), KU Leuven, Willem de Croylaan 42, bus 2428, 3001, Leuven, Belgium.
authors.forename: Elias A
authors.initials: EA
authors.lastname: Bekele
authors.affiliation: Department of Biosystems (BIOSYST), Division of Mechatronics, Biostatistics and Sensors (MeBioS), KU Leuven, Willem de Croylaan 42, bus 2428, 3001, Leuven, Belgium.
authors.forename: Wasiye F
authors.initials: WF
authors.lastname: Beshir
authors.affiliation: Department of Biosystems (BIOSYST), Division of Mechatronics, Biostatistics and Sensors (MeBioS), KU Leuven, Willem de Croylaan 42, bus 2428, 3001, Leuven, Belgium.
authors.forename: Maarten L A T M
authors.initials: ML
authors.lastname: Hertog
authors.affiliation: Department of Biosystems (BIOSYST), Division of Mechatronics, Biostatistics and Sensors (MeBioS), KU Leuven, Willem de Croylaan 42, bus 2428, 3001, Leuven, Belgium.
authors.forename: Bart M
authors.initials: BM
authors.lastname: Nicolai
authors.affiliation: Department of Biosystems (BIOSYST), Division of Mechatronics, Biostatistics and Sensors (MeBioS), KU Leuven, Willem de Croylaan 42, bus 2428, 3001, Leuven, Belgium.
authors.forename: Annemie H
authors.initials: AH
authors.lastname: Geeraerd
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology*; D000596:Amino Acids / Q000378:metabolism; D050260:Carbohydrate Metabolism; D003521:Cyclopropanes / Q000494:pharmacology; D004780:Environment, Controlled; D005030:Ethylenes / Q000378:metabolism*; D027845:Malus / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D055432:Metabolomics / Q000379:methods; D010100:Oxygen / Q000378:metabolism; D013312:Stress, Physiological / Q000502:physiology
chemical_list: D000596:Amino Acids; D003521:Cyclopropanes; D005030:Ethylenes; C036216:ethylene; C412563:1-methylcyclopropene; D010100:Oxygen