title: Antimicrobial action of an endophytic fungi fromSophor flavescensand structure identification of its active constituent.
abstract: Endophytic fungus BS002 was isolated and characterized fromSophora flavescensby plate method, which has broad antimicrobial activity. Isolation and trace of a new bioactive compound from the fungus' culture extracts with the method of column chromatography and TLC biological autoradiography was conducted. Finally, it was identified as 6,7-(2'E) dibutenyl-5,8-dihydroxy-(Z)-cyclooct-2-ene-1,4-dione by nuclear magnetic resonance, infrared and liquid chromatography-mass spectrometry. The compound presented strong antifungal activities for example:Botryosphaeria berengrianaf.sp.piricola, Physalospora piricola, Cladosporium cucumerinumEll. Arthur., Fusarium oxysporum f.sp. cucumerinum, Fusarium moniliforme.The inhibition toPhysalospora piricolawas the strongest with an antibacterial diameter of 45 mm. This paper is the first report of the antimicrobial activity of endophytic fungi BS002 that was the secondary metabolites extracted from the seeds ofSophora flavescens. The results provide a broad foreground for biopharmaceuticals and biopesticide.
authors.affiliation: School of Chemical Engineering, University of Science and Technology Liaoning, Anshan City, Liaoning Province , People's Republic of China.
authors.forename: Na
authors.initials: N
authors.lastname: Yu
authors.affiliation: School of Chemical Engineering, University of Science and Technology Liaoning, Anshan City, Liaoning Province , People's Republic of China.
authors.forename: Lu
authors.initials: L
authors.lastname: He
authors.affiliation: Shanghai Key Lab of Protected Horticultural Technology, Shanghai Academy of Agricultural Sciences, Horticultural Research Institute, Shanghai City , China People's Republic of China.
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.affiliation: School of Chemical Engineering, University of Science and Technology Liaoning, Anshan City, Liaoning Province , People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Chemical Engineering, University of Science and Technology Liaoning, Anshan City, Liaoning Province , People's Republic of China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Xu
authors.affiliation: School of Chemical Engineering, University of Science and Technology Liaoning, Anshan City, Liaoning Province , People's Republic of China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Liu
keywords: Sophora flavescens; TLC biological autoradiography; antimicrobial substance; endophytic fungi; isolation; structure analysis