title: Simultaneous determination of acetylpuerarin and puerarin in rat plasma by liquid chromatography-tandem mass spectrometry: Application to a pharmacokinetic study following intravenous and oral administration.
abstract: A rapid and sensitive liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed and validated for the simultaneous determination of acetylpuerarin (AP) and its major metabolite puerarin (PUE) in rat plasma using genistein as the internal standard (IS). Plasma samples were pretreated by protein precipitation with a mixture of methanol and acetonitrile. Chromatographic separation was performed on a CAPCELL PAK C18 MGШ column with a mixture of 0.1% formic acid in water and methanol (35:65, v/v) as the mobile phase. The analytes were detected using a tandem mass spectrometer in the positive ionization and multiple-reaction monitoring mode. The ion transition of m/z 669.4→627.3, 417.5→297.6 and 271.3→153.0 was utilized to quantify AP, PUE and the IS, respectively. The calibration curves showed good linearity over the plasma concentration range of 1-2000ng/mL for AP and 2.5-5000ng/mL for PUE. The intra- and inter-day precisions (RSD %) for each analyte were less than 6.91%, and the accuracies ranged from -2.17% to 2.93%. The validated LC-MS/MS method was further successfully applied to a pharmacokinetic study of AP and PUE in rats following intravenous and oral administration.
authors.affiliation: Department of Pharmacy, Jinan 250033, China. Electronic address: sundq0405@126.com.
authors.forename: Deqing
authors.initials: D
authors.lastname: Sun
authors.affiliation: Department of Cardiology, Second Hospital of Shandong University, Jinan 250033, China.
authors.forename: Aiying
authors.initials: A
authors.lastname: Xue
authors.affiliation: Department of Pharmacy, Jinan 250033, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Pharmacy, Jinan 250033, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Pharmacy, Jinan 250033, China.
authors.forename: Jinlong
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Pharmacy, Jinan 250033, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Pharmacy, Jinan 250033, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Sun
mesh_terms: D061605:Administration, Intravenous; D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D007529:Isoflavones / Q000008:administration & dosage* / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D016014:Linear Models; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D007529:Isoflavones; C521069:acetylpuerarin; C033607:puerarin
keywords: Acetylpuerarin; LC–MS/MS; Pharmacokinetics; Puerarin