title: Promoter-Autonomous Functioning in a Controlled Environment using Single Molecule FISH.
abstract: Transcription is a highly regulated biological process, initiated through the assembly of complexes at the promoter that contain both the general transcriptional machinery and promoter-specific factors. Despite the abundance of studies focusing on transcription, certain questions have remained unanswered. It is not clear how the transcriptional profile of a promoter is affected by genomic context. Also, there is no single cell method to directly compare transcriptional profiles independent of gene length and sequence. In this work, we employ a single genetic site for isolating the transcriptional kinetics of yeast promoters. Utilizing single molecule FISH, we directly compare the transcriptional activity of different promoters, considering both synthesis and cell-to-cell variability. With this approach, we provide evidence suggesting promoters autonomously encode their associated transcriptional profiles, independent of genomic locus, gene length and gene sequence.
authors.affiliation: Department of Anatomy and Structural Biology, Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, New York, USA.
authors.forename: Sami
authors.initials: S
authors.lastname: Hocine
authors.affiliation: Department of Anatomy and Structural Biology, Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, New York, USA.
authors.forename: Maria
authors.initials: M
authors.lastname: Vera
authors.affiliation: Department of Biochemistry, Université de Montréal, Montreal, Canada.
authors.forename: Daniel
authors.initials: D
authors.lastname: Zenklusen
authors.affiliation: Department of Anatomy and Structural Biology, Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, New York, USA.
authors.forename: Robert H
authors.initials: RH
authors.lastname: Singer
mesh_terms: D005686:Galactokinase / Q000235:genetics; D056426:Genetic Loci; D006360:Heat-Shock Proteins / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D008856:Microscopy, Fluorescence; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D014158:Transcription, Genetic; D059467:Transcriptome
chemical_list: D006360:Heat-Shock Proteins; D029701:Saccharomyces cerevisiae Proteins; C070972:HsP104 protein, S cerevisiae; C524219:GAL1 protein, S cerevisiae; D005686:Galactokinase