title: Rapid Detection of KPC, NDM, and OXA-48-Like Carbapenemases by Real-Time PCR from Rectal Swab Surveillance Samples.
abstract: We describe a multiplex real-time PCR assay for use on the ABI 7500 Fast TaqMan platform to detect all currently described Klebsiella pneumoniae carbapenemases (KPC), New Delhi metallo-β-lactamases (NDM), and the OXA-48-like family of carbapenemases from bacterial culture lysates or sample enrichment broth lysates.
authors.affiliation: Public Health Microbiology and Reference Laboratory, British Columbia Centre for Disease Control, PHSA Laboratories, Vancouver, British Columbia, Canada.
authors.forename: Tracy D
authors.initials: TD
authors.lastname: Lee
authors.affiliation: Public Health Microbiology and Reference Laboratory, British Columbia Centre for Disease Control, PHSA Laboratories, Vancouver, British Columbia, Canada.
authors.forename: Kathleen
authors.initials: K
authors.lastname: Adie
authors.affiliation: Public Health Microbiology and Reference Laboratory, British Columbia Centre for Disease Control, PHSA Laboratories, Vancouver, British Columbia, Canada.
authors.forename: Alan
authors.initials: A
authors.lastname: McNabb
authors.affiliation: Department of Pathology and Laboratory Medicine, Faculty of Medicine, University of British Columbia, Vancouver, British Columbia, Canada Department of Laboratory Medicine and Pathology, Fraser Health, Surrey, British Columbia, Canada.
authors.forename: Dale
authors.initials: D
authors.lastname: Purych
authors.affiliation: Department of Laboratory Medicine and Pathology, Fraser Health, Surrey, British Columbia, Canada.
authors.forename: Kulvinder
authors.initials: K
authors.lastname: Mannan
authors.affiliation: Public Health Microbiology and Reference Laboratory, British Columbia Centre for Disease Control, PHSA Laboratories, Vancouver, British Columbia, Canada.
authors.forename: Robert
authors.initials: R
authors.lastname: Azana
authors.affiliation: Public Health Microbiology and Reference Laboratory, British Columbia Centre for Disease Control, PHSA Laboratories, Vancouver, British Columbia, Canada.
authors.forename: Corrinne
authors.initials: C
authors.lastname: Ng
authors.affiliation: Public Health Microbiology and Reference Laboratory, British Columbia Centre for Disease Control, PHSA Laboratories, Vancouver, British Columbia, Canada Department of Pathology and Laboratory Medicine, Faculty of Medicine, University of British Columbia, Vancouver, British Columbia, Canada.
authors.forename: Patrick
authors.initials: P
authors.lastname: Tang
authors.affiliation: Public Health Microbiology and Reference Laboratory, British Columbia Centre for Disease Control, PHSA Laboratories, Vancouver, British Columbia, Canada Department of Pathology and Laboratory Medicine, Faculty of Medicine, University of British Columbia, Vancouver, British Columbia, Canada linda.hoang@bccdc.ca.
authors.forename: Linda M N
authors.initials: LM
authors.lastname: Hoang
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics; D062665:Epidemiological Monitoring; D006090:Gram-Negative Bacteria / Q000201:enzymology* / Q000235:genetics; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012007:Rectum / Q000382:microbiology*; D001618:beta-Lactamases / Q000032:analysis* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase