title: Crosstalk between platelets and PBMC: New evidence in wound healing.
abstract: Platelet-derived products have proven useful in accelerating healing processes and tissue regeneration. However, despite their widespread use in clinical practice, the cellular and molecular mechanisms involved have not yet been completely clarified. Recent studies show that interaction between platelet gel (PG) and peripheral blood mononuclear cells (PBMC) can result in activation of PBMC and production of several cytokines involved in wound healing and tissue repair. The aim of our study was to analyze whether crosstalk between platelets and PBMC can influence wound healing by modulating release of VEGF, bFGF and IL-10 by PBMC. Cultures of PBMC alone and co-cultures with autologous PG of 24 healthy volunteers were incubated under normoxia for 24 h. VEGF, bFGF and IL-10 concentration and expression were then analyzed in supernatants by ELISA and by real-time RT-PCR. We observed a down-regulation of VEGF and bFGF release and an up-regulation of IL-10 release in co-cultures of PBMC and PG. Platelets are not only important in the early stages of the healing process (clot formation, direct release of growth factors), but also can influence the whole process of tissue regeneration by modulating synthesis and release of VEGF, bFGF and IL-10 by PBMC. These effects could give platelets a new key role in the control of healing processes and provide insights into the clinical success of platelet-derived products in many medical fields.
authors.affiliation: a Department of Medical , Surgical and Neuro Sciences, Dermatology Section, University of Siena , Siena , Italy .
authors.forename: Niccolò
authors.initials: N
authors.lastname: Nami
authors.affiliation: a Department of Medical , Surgical and Neuro Sciences, Dermatology Section, University of Siena , Siena , Italy .
authors.forename: Luca
authors.initials: L
authors.lastname: Feci
authors.affiliation: b Department of Medical , Surgical and Neuro Sciences, Human Pathology Section, University of Siena , Siena , Italy .
authors.forename: Luca
authors.initials: L
authors.lastname: Napoliello
authors.affiliation: b Department of Medical , Surgical and Neuro Sciences, Human Pathology Section, University of Siena , Siena , Italy .|c Molecular Medicine, Center for Biotechnology, College of Science and Technology, Temple University , Philadelphia , PA USA , and.
authors.forename: Antonio
authors.initials: A
authors.lastname: Giordano
authors.affiliation: d Department of Medical , Surgical and Neuro Sciences, Rheumatology Section, University of Siena , Siena , Italy.
authors.forename: Sauro
authors.initials: S
authors.lastname: Lorenzini
authors.affiliation: d Department of Medical , Surgical and Neuro Sciences, Rheumatology Section, University of Siena , Siena , Italy.
authors.forename: Mauro
authors.initials: M
authors.lastname: Galeazzi
authors.affiliation: a Department of Medical , Surgical and Neuro Sciences, Dermatology Section, University of Siena , Siena , Italy .
authors.forename: Pietro
authors.initials: P
authors.lastname: Rubegni
authors.affiliation: a Department of Medical , Surgical and Neuro Sciences, Dermatology Section, University of Siena , Siena , Italy .
authors.forename: Michele
authors.initials: M
authors.lastname: Fimiani
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism*; D002450:Cell Communication; D018920:Coculture Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000235:genetics* / Q000378:metabolism; D005782:Gels; D005786:Gene Expression Regulation; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics* / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D015539:Platelet Activation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics* / Q000378:metabolism; D014945:Wound Healing
chemical_list: D005782:Gels; C508609:IL10 protein, human; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2; D016753:Interleukin-10
keywords: PBMC; platelet gel; platelets; tissue repair; wound healing