title: Ex vivo confocal microscopy imaging to identify tumor tissue on freshly removed brain sample.
abstract: Confocal microscopy is a technique able to realize "optic sections" of a tissue with increasing applications. We wondered if we could apply an ex vivo confocal microscope designed for dermatological purpose in a routine use for the most frequent brain tumors. The aim of this work was to identify tumor tissue and its histopathological hallmarks, and to assess grading criteria used in neuropathological practice without tissue loss on freshly removed brain tissue. Seven infiltrating gliomas, nine meningiomas and three metastases of carcinomas were included. We compared imaging results obtained with the confocal microscope to frozen sections, smears and tissue sections of formalin-fixed tissue. Our results show that ex vivo confocal microscopy imaging can be applied to brain tumors in order to quickly identify tumor tissue without tissue loss. It can differentiate tumors and can assess most of grading criteria. Confocal microscopy could represent a new tool to identify tumor tissue on freshly removed sample and could help in selecting areas for biobanking of tumor tissue.
authors.affiliation: Service d'Anatomie et Cytologie Pathologiques - CHU de Saint Étienne, Hôpital Nord, 42055, Saint Étienne. Cedex2, France. fabien.forest@chu-st-etienne.fr.
authors.forename: Fabien
authors.initials: F
authors.lastname: Forest
authors.affiliation: Service de Dermatologie - CHU de Saint Étienne, Hôpital Nord, 42055, Saint Étienne. Cedex2, France.
authors.forename: Elisa
authors.initials: E
authors.lastname: Cinotti
authors.affiliation: Service d'Anatomie et Cytologie Pathologiques - CHU de Saint Étienne, Hôpital Nord, 42055, Saint Étienne. Cedex2, France.
authors.forename: Violaine
authors.initials: V
authors.lastname: Yvorel
authors.affiliation: Service d'Anatomie et Cytologie Pathologiques - CHU de Saint Étienne, Hôpital Nord, 42055, Saint Étienne. Cedex2, France.
authors.forename: Cyril
authors.initials: C
authors.lastname: Habougit
authors.affiliation: Service de Neurochirurgie - CHU de Saint Étienne, Hôpital Nord, 42055, Saint Étienne. Cedex2, France.
authors.forename: François
authors.initials: F
authors.lastname: Vassal
authors.affiliation: Service de Neurochirurgie - CHU de Saint Étienne, Hôpital Nord, 42055, Saint Étienne. Cedex2, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Nuti
authors.affiliation: Service de Dermatologie - CHU de Saint Étienne, Hôpital Nord, 42055, Saint Étienne. Cedex2, France.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Perrot
authors.affiliation: Service de Dermatologie - CHU de Saint Étienne, Hôpital Nord, 42055, Saint Étienne. Cedex2, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Labeille
authors.affiliation: Service d'Anatomie et Cytologie Pathologiques - CHU de Saint Étienne, Hôpital Nord, 42055, Saint Étienne. Cedex2, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Péoc'h
mesh_terms: D001921:Brain / Q000473:pathology* / Q000601:surgery; D001932:Brain Neoplasms / Q000175:diagnosis* / Q000473:pathology* / Q000601:surgery; D002277:Carcinoma / Q000175:diagnosis / Q000473:pathology / Q000601:surgery; D015925:Cryopreservation; D005557:Formaldehyde; D005910:Glioma / Q000175:diagnosis / Q000473:pathology / Q000601:surgery; D006801:Humans; D008579:Meningioma / Q000175:diagnosis / Q000473:pathology / Q000601:surgery; D018613:Microscopy, Confocal / Q000379:methods*; D060787:Neoplasm Grading; D016612:Paraffin Embedding; D016707:Tissue Fixation
chemical_list: D005557:Formaldehyde
keywords: Brain metastasis; Confocal reflectance microscopy; Glial neoplasms; Meningioma