title: [Protective effect of heart-fatty acid binding protein on lipopolysaccharide-induced cardiomyocyte damage].
abstract: OBJECTIVE
  To observe the protective effect of heart-fatty acid binding protein (H-FABP) on lipopolysaccharide (LPS)-induced cardiomyocyte damage.
  
  
  METHODS
  The cardiomyocytes were isolated and cultured from 1-3 days old neonatal rats. The specific siRNA or plasmid of H-FABP were transfected into cells to alter H-FABP expression, which was evaluated by Western blot and quantitative-PCR. LPS-induced cardiomyocyte damage and inflammation were estimated by detecting the contents of lactate dehydrogenase(LDH), TNF-α, and IL-1β as well as cell viability.
  
  
  RESULTS
  LPS treatment induced inflammation and cell damage indicated by a decrease in cell viability and an increase in LDH, TNF-α and IL-1β in the medium. When H-FABP was downregulated by siRNA transfection, the LPS-induced inflammation and cell damage were augmented. In contrast, when H-FABP was overexpressed by pcDNA3.1-H-FABP transfection, the LPS-induced inflammation and cell damage were suppressed.
  
  
  CONCLUSIONS
  H-FABP protects cardiomyocytes from LPS-induced inflammation and cell injury.
authors.affiliation: Department of Infectious Disease, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.forename: Kangkai
authors.initials: K
authors.lastname: Wang
authors.forename: Yongfang
authors.initials: Y
authors.lastname: Jiang
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002460:Cell Line; D002470:Cell Survival; D015536:Down-Regulation; D000074026:Fatty Acid Binding Protein 3; D050556:Fatty Acid-Binding Proteins / Q000378:metabolism*; D007249:Inflammation / Q000378:metabolism*; D053583:Interleukin-1beta / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008070:Lipopolysaccharides / Q000009:adverse effects; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000187:drug effects; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: C509948:FABP3 protein, rat; D000074026:Fatty Acid Binding Protein 3; D050556:Fatty Acid-Binding Proteins; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D034741:RNA, Small Interfering; D014409:Tumor Necrosis Factor-alpha; D007770:L-Lactate Dehydrogenase