title: Effects of Inhaled Corticosteroids on the Growth Plates of Infant Rats.
abstract: The most significant adverse effect of inhaled steroid administration in children is suppression of hypothalamic-pituitary-adrenal axis responsiveness and suppression of growth. This study evaluates the effects of inhaled corticosteroids on the growth plates in infant rats. Rats aged 10 days were divided into five groups. Low and high doses of budesonide and fluticasone propionate (50-200-250 mcg/day) were applied with a modified spacer for 10 days. The rat's tibias were then removed and the effects of the steroids on the growth plates were compared. Growth cartilage chondrocyte proliferation and apoptosis rates; IGF-1 and glucocorticoid receptor levels; and resting, proliferative, hypertrophic, and total zone (TZ) measurements were compared using immunohistochemical-staining methods. With high doses of fluticasone, growth plates were affected much more than with high doses of budesonide (p = 0.01). Fluticasone, particularly at a dose of 250 mcg, inhibited the growth plate with an intensive negative impact on all parameters.
authors.affiliation: Department of Pediatrics, Van Military Hospital , Van , Turkey.
authors.forename: Serkan
authors.initials: S
authors.lastname: Kemer
authors.forename: Ferhan
authors.initials: F
authors.lastname: Karademir
authors.forename: Gokhan
authors.initials: G
authors.lastname: Aydemir
authors.forename: Zafer
authors.initials: Z
authors.lastname: Kucukodaci
authors.forename: Ozgur
authors.initials: O
authors.lastname: Pirgon
authors.forename: F Alparslan
authors.initials: FA
authors.lastname: Genc
authors.forename: Secil
authors.initials: S
authors.lastname: Aydinoz
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D000833:Animals, Suckling; D001835:Body Weight / Q000187:drug effects; D019819:Budesonide / Q000008:administration & dosage / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D019902:Chondrocytes / Q000187:drug effects / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D000068298:Fluticasone / Q000008:administration & dosage / Q000633:toxicity*; D006132:Growth Plate / Q000737:chemistry / Q000187:drug effects*; D006984:Hypertrophy; D007030:Hypothalamo-Hypophyseal System / Q000187:drug effects; D007334:Insulin-Like Growth Factor I / Q000032:analysis; D010012:Osteogenesis / Q000187:drug effects; D010913:Pituitary-Adrenal System / Q000187:drug effects; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D011965:Receptors, Glucocorticoid / Q000032:analysis; D013977:Tibia / Q000187:drug effects / Q000254:growth & development
chemical_list: D011965:Receptors, Glucocorticoid; C548904:insulin-like growth factor-1, rat; D019819:Budesonide; D007334:Insulin-Like Growth Factor I; D000068298:Fluticasone
keywords: budesonide; fluticasone propionate; glucocorticoids; growth plate; infant rats