title: Effect of internal limiting membrane abrasion on retinal tissues in macular holes.
abstract: OBJECTIVE
  The purpose of this study was to identify the structural and histological effects of a Tano diamond-dusted membrane scraper (DDMS) on the retinal surface after internal limiting membrane (ILM) abrasion in macular hole surgery.
  
  
  METHODS
  Institutional experimental study was performed in 11 eyes. All eyes underwent ILM abrasion in the operating room with a DDMS for macular hole repair as an alternative to traditional ILM peeling. Three human donor eyes underwent an identical procedure in the laboratory. Retinal tissues were removed by ILM abrasion with a DDMS during vitrectomy for macular hole repair and retinal tissues remaining in human donor eyes. Main outcome measures were microscopic and immunohistological characteristics of instrument tip tissues and retinal structure after ILM abrasion.
  
  
  RESULTS
  The tips of the Tano DDMS showed evidence of cellular membranes and ILM removal. The retinas showed distinct areas of lamellar ILM removal without penetration of the retinal nerve fiber layer (RNFL).
  
  
  CONCLUSIONS
  Application of the Tano DDMS instrument is sufficient to remove membranes from the surface of the ILM and layers of the ILM without disruption of the underlying RNFL. Internal limiting membrane abrasion can be a useful and effective alternative to complete ILM removal for macular surgery.
authors.affiliation: Department of Ophthalmology and Visual Sciences, University of Iowa Hospitals and Clinics, Iowa City, Iowa, United States.
authors.forename: David R P
authors.initials: DR
authors.lastname: Almeida
authors.affiliation: Department of Ophthalmology and Visual Sciences, University of Iowa Hospitals and Clinics, Iowa City, Iowa, United States.
authors.forename: Eric K
authors.initials: EK
authors.lastname: Chin
authors.affiliation: Department of Ophthalmology and Visual Sciences, University of Iowa Hospitals and Clinics, Iowa City, Iowa, United States.
authors.forename: Ryan M
authors.initials: RM
authors.lastname: Tarantola
authors.affiliation: Department of Ophthalmology and Visual Sciences, University of Iowa Hospitals and Clinics, Iowa City, Iowa, United States.
authors.forename: James C
authors.initials: JC
authors.lastname: Folk
authors.affiliation: Department of Ophthalmology and Visual Sciences, University of Iowa Hospitals and Clinics, Iowa City, Iowa, United States.
authors.forename: H Culver
authors.initials: HC
authors.lastname: Boldt
authors.affiliation: Department of Ophthalmology and Visual Sciences, University of Iowa Hospitals and Clinics, Iowa City, Iowa, United States 2Omics Laboratory, Iowa City, Iowa, United States.
authors.forename: Jessica M
authors.initials: JM
authors.lastname: Skeie
authors.affiliation: Department of Ophthalmology and Visual Sciences, University of Iowa Hospitals and Clinics, Iowa City, Iowa, United States.
authors.forename: Robert F
authors.initials: RF
authors.lastname: Mullins
authors.affiliation: Department of Ophthalmology and Visual Sciences, University of Iowa Hospitals and Clinics, Iowa City, Iowa, United States.
authors.forename: Stephen R
authors.initials: SR
authors.lastname: Russell
authors.affiliation: Department of Ophthalmology and Visual Sciences, University of Iowa Hospitals and Clinics, Iowa City, Iowa, United States 2Omics Laboratory, Iowa City, Iowa, United States.
authors.forename: Vinit B
authors.initials: VB
authors.lastname: Mahajan
mesh_terms: D000368:Aged; D019773:Epiretinal Membrane / Q000473:pathology / Q000601:surgery*; D005260:Female; D006801:Humans; D008266:Macula Lutea / Q000648:ultrastructure; D008297:Male; D064347:Microscopy, Electrochemical, Scanning; D008875:Middle Aged; D012167:Retinal Perforations / Q000473:pathology / Q000601:surgery*; D014821:Vitrectomy / Q000295:instrumentation / Q000379:methods