title: [Expression of CD163 in children with Epstein-Barr virus infection].
abstract: OBJECTIVE
  To study the clinical significance of CD163 in the diagnosis and the evaluation of severity and prognosis of childhood hemophagocytic lymphohistiocytosis (HLH).
  
  
  METHODS
  Ninety-four children were classified into Epstein-Barr virus (EBV)-positive (n=55) and EBV-negative groups (n=39; control group). The EBV-positive group was subgrouped into infectious mononucleosis (IM; n=47) and HLH (n=8). Serum levels of soluble CD163 were measured using ELISA. Expression of CD163 on mononuclear cells was detected by flow cytometry.
  
  
  RESULTS
  The serum levels of soluble CD163 were>10 000 ng/mL in all eight HLH patients (>30 000 ng/mL in 3 cases). The mean serum levels of soluble CD163 in the HLH group were significantly higher than in the control and IM groups (P<0.05). The serum levels of soluble CD163 in EBV-positive children were positively correlated with EBV-DNA copies and serum levels of ferritin and LDH, but were negatively correlated with white blood cell count, neutrophil count, hemoglobin and platelet count. The follow-up after treatment for three HLH patients showed that serum levels of soluble CD163 were significantly reduced, but the soluble CD163 levels rebounded in one patient who was complicated by fungal pneumonia infection.
  
  
  CONCLUSIONS
  The levels of serum soluble CD163 may be related to the severity in children with HLH. The EBV-positive children with soluble CD163 levels >10 000 ng/mL should be considered the possibility of HLH.
authors.affiliation: Department of Pediatrics, Fifth Hospital of Guangzhou Medical College, Guangzhou 510700, China. dengchangbo@163.com.
authors.forename: Yan-Li
authors.initials: YL
authors.lastname: Chen
authors.forename: Fu-Xiong
authors.initials: FX
authors.lastname: Chen
authors.forename: Chang-Bo
authors.initials: CB
authors.lastname: Deng
authors.forename: Bo
authors.initials: B
authors.lastname: Xia
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Wu
authors.forename: Ze-Lin
authors.initials: ZL
authors.lastname: Wu
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Lu
mesh_terms: D000293:Adolescent; D015703:Antigens, CD / Q000032:analysis*; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D020031:Epstein-Barr Virus Infections / Q000276:immunology*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D011956:Receptors, Cell Surface / Q000032:analysis*; D000098902:CD163 Antigen
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D000098902:CD163 Antigen; D011956:Receptors, Cell Surface