title: The Peripheral Olfactory Repertoire of the Lightbrown Apple Moth, Epiphyas postvittana.
abstract: The lightbrown apple moth, Epiphyas postvittana is an increasingly global pest of horticultural crops. Like other moths, E. postvittana relies on olfactory cues to locate mates and oviposition sites. To detect these cues, moths have evolved families of genes encoding elements of the peripheral olfactory reception system, including odor carriers, receptors and degrading enzymes. Here we undertake a transcriptomic approach to identify members of these families expressed in the adult antennae of E. postvittana, describing open reading frames encoding 34 odorant binding proteins, 13 chemosensory proteins, 70 odorant receptors, 19 ionotropic receptors, nine gustatory receptors, two sensory neuron membrane proteins, 27 carboxylesterases, 20 glutathione-S-transferases, 49 cytochrome p450s and 18 takeout proteins. For the odorant receptors, quantitative RT-PCR corroborated RNAseq count data on steady state transcript levels. Of the eight odorant receptors that group phylogenetically with pheromone receptors from other moths, two displayed significant male-biased expression patterns, one displayed significant female-biased expression pattern and five were expressed equally in the antennae of both sexes. In addition, we found two male-biased odorant receptors that did not group with previously described pheromone receptors. This suite of olfaction-related genes provides a substantial resource for the functional characterization of this signal transduction system and the development of odor-mediated control strategies for horticultural pests.
authors.affiliation: School of Biological Sciences, University of Auckland, Auckland, New Zealand; The New Zealand Institute for Plant & Food Research Ltd, Auckland, New Zealand.
authors.forename: Jacob A
authors.initials: JA
authors.lastname: Corcoran
authors.affiliation: The New Zealand Institute for Plant & Food Research Ltd, Auckland, New Zealand.
authors.forename: Melissa D
authors.initials: MD
authors.lastname: Jordan
authors.affiliation: The New Zealand Institute for Plant & Food Research Ltd, Auckland, New Zealand.
authors.forename: Amali H
authors.initials: AH
authors.lastname: Thrimawithana
authors.affiliation: The New Zealand Institute for Plant & Food Research Ltd, Auckland, New Zealand.
authors.forename: Ross N
authors.initials: RN
authors.lastname: Crowhurst
authors.affiliation: School of Biological Sciences, University of Auckland, Auckland, New Zealand; The New Zealand Institute for Plant & Food Research Ltd, Auckland, New Zealand.
authors.forename: Richard D
authors.initials: RD
authors.lastname: Newcomb
mesh_terms: D000818:Animals; D057506:Arthropod Antennae / Q000502:physiology; D020869:Gene Expression Profiling / Q000379:methods; D017344:Genes, Insect / Q000235:genetics; D019476:Insect Proteins / Q000235:genetics; D009036:Moths / Q000235:genetics*; D009812:Odorants; D010802:Phylogeny; D018035:Receptors, Odorant / Q000235:genetics*; D043563:Receptors, Pheromone / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics; D012903:Smell / Q000235:genetics* / Q000502:physiology; D059467:Transcriptome / Q000235:genetics
chemical_list: D019476:Insect Proteins; D018035:Receptors, Odorant; D043563:Receptors, Pheromone; C045648:odorant-binding protein