title: Detection of Waterborne Viruses Using High Affinity Molecularly Imprinted Polymers.
abstract: Molecularly imprinted polymers (MIPs) are artificial receptor ligands which can recognize and specifically bind to a target molecule. They are more resistant to chemical and biological damage and inactivation than antibodies. Therefore, target specific-MIP nanoparticles are aimed to develop and implemented to biosensors for the detection of biological toxic agents such as viruses, bacteria, and fungi toxins that cause many diseases and death due to the environmental contamination. For the first time, a molecularly imprinted polymer (MIP) targeting the bacteriophage MS2 as the template was investigated using a novel solid-phase synthesis method to obtain the artificial affinity ligand for the detection and removal of waterborne viruses through optical-based sensors. A high affinity between the artificial ligand and the target was found, and a regenerative MIP-based virus detection assay was successfully developed using a new surface plasmon resonance (SPR)-biosensor which provides an alternative technology for the specific detection and removal of waterborne viruses that lead to high disease and death rates all over the world.
authors.affiliation: †Biomedical Engineering, Cranfield University, Bedfordshire MK43 0AL, United Kingdom.
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Altintas
authors.affiliation: †Biomedical Engineering, Cranfield University, Bedfordshire MK43 0AL, United Kingdom.
authors.forename: Micah
authors.initials: M
authors.lastname: Gittens
authors.affiliation: †Biomedical Engineering, Cranfield University, Bedfordshire MK43 0AL, United Kingdom.
authors.forename: Antonio
authors.initials: A
authors.lastname: Guerreiro
authors.affiliation: ‡Public Health England, Porton Down, Salisbury SP4 0JG, United Kingdom.
authors.forename: Katy-Anne
authors.initials: KA
authors.lastname: Thompson
authors.affiliation: ‡Public Health England, Porton Down, Salisbury SP4 0JG, United Kingdom.
authors.forename: Jimmy
authors.initials: J
authors.lastname: Walker
authors.affiliation: †Biomedical Engineering, Cranfield University, Bedfordshire MK43 0AL, United Kingdom.
authors.forename: Sergey
authors.initials: S
authors.lastname: Piletsky
authors.affiliation: †Biomedical Engineering, Cranfield University, Bedfordshire MK43 0AL, United Kingdom.
authors.forename: Ibtisam E
authors.initials: IE
authors.lastname: Tothill
mesh_terms: D046529:Microscopy, Electron, Transmission; D054802:Molecular Imprinting; D011108:Polymers / Q000737:chemistry*; D014780:Viruses / Q000302:isolation & purification*; D014871:Water Microbiology
chemical_list: D011108:Polymers