title: Cellular superresolved imaging of multiple markers using temporally flickering nanoparticles.
abstract: In this paper we present a technique aimed for simultaneous detection of multiple types of gold nanoparticles (GNPs) within a biological sample, using lock-in detection. We image the sample using a number of modulated laser beams that correspond to the number of GNP species that label a given sample. The final image where the GNPs are spatially separated is obtained computationally. The proposed method enables the simultaneous superresolved imaging of different areas of interest within biological sample and also the spatial separation of GNPs at sub-diffraction distances, making it a useful tool in the study of intracellular trafficking pathways in living cells.
authors.affiliation: 1] Faculty of Engineering, Bar Ilan University, Ramat-Gan 5290002, Israel [2] The Bar-Ilan Institute of Nanotechnology &Advanced Materials, Bar Ilan University, Ramat-Gan 5290002, Israel.
authors.forename: Tali
authors.initials: T
authors.lastname: Ilovitsh
authors.affiliation: 1] Faculty of Engineering, Bar Ilan University, Ramat-Gan 5290002, Israel [2] The Bar-Ilan Institute of Nanotechnology &Advanced Materials, Bar Ilan University, Ramat-Gan 5290002, Israel.
authors.forename: Yossef
authors.initials: Y
authors.lastname: Danan
authors.affiliation: 1] Faculty of Engineering, Bar Ilan University, Ramat-Gan 5290002, Israel [2] The Bar-Ilan Institute of Nanotechnology &Advanced Materials, Bar Ilan University, Ramat-Gan 5290002, Israel.
authors.forename: Rinat
authors.initials: R
authors.lastname: Meir
authors.affiliation: Department of Electrical and Computer Engineering, University of Utah, Salt Lake City, Utah, USA.
authors.forename: Amihai
authors.initials: A
authors.lastname: Meiri
authors.affiliation: 1] Faculty of Engineering, Bar Ilan University, Ramat-Gan 5290002, Israel [2] The Bar-Ilan Institute of Nanotechnology &Advanced Materials, Bar Ilan University, Ramat-Gan 5290002, Israel.
authors.forename: Zeev
authors.initials: Z
authors.lastname: Zalevsky