title: miRNAs in Sera of Tunisian patients discriminate between inflammatory breast cancer and non-inflammatory breast cancer.
abstract: In recent years, circulating miRNAs have attracted interest as stable, non-invasive biomarkers for various pathological conditions. Here, we investigated their potential to serve as minimally invasive, early detection markers for inflammatory breast cancer (IBC) and non-inflammatory breast cancer (non-IBC) in serum. miRNA profiling was performed on serum from 20 patients with non-IBC, 20 with IBC, and 20 normal control subjects. Real-time reverse transcription-polymerase chain reaction (qRT-PCR) was applied to measure the level of 12 candidate miRNAs previously identified in other research(miR-342-5p, miR-342--3p, miR-320, miR-30b, miR-29a, miR-24, miR-15a, miR-548d-5p, miR-486-3p, miR-451, miR-337-5p, miR-335).We found that 4 miRNAs (miR-24, miR-342-3p, miR-337-5p and miR-451) were differentially expressed in serum of IBC patients compared to non-IBC, and 3 miRNAs (miR-337-5p ,miR-451and miR-30b) were differentially expressed in IBC and non-IBC patients combined compared to healthy controls. miR-24, miR-342-3p, miR-337-5p and miR-451 were found to be significantly down-regulated in IBC patients compared to non-IBC. Likewise, the expression level of mir-451 showed significant down-regulation in IBC serum, while mir-30b and miR-337-5p were up-regulated in non-IBC serum comparatively to normal controls. Using receiver operational curve (ROC) analysis, we show that dysregulated miRNAs can discriminate patients with IBC and non-IBC from healthy controls with sensitivity ranging from 76 to 81% and specificity from 66 to 80%, for three separate miRNAs. In conclusion, our data suggest that circulating miRNAs are potential biomarkers for classifying IBC and non-IBC, and may also be candidates for early detection of breast cancer.
authors.affiliation: Laboratory of Genetics, Immunology and Human Pathology, Faculty of Sciences, University of Tunis El Manar, El Mannar I, Tunis, 2092 Tunisia.
authors.forename: Khouloud
authors.initials: K
authors.lastname: Hamdi
authors.affiliation: Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC 20007 USA.
authors.forename: David
authors.initials: D
authors.lastname: Goerlitz
authors.affiliation: Laboratory of Genetics, Immunology and Human Pathology, Faculty of Sciences, University of Tunis El Manar, El Mannar I, Tunis, 2092 Tunisia.
authors.forename: Neila
authors.initials: N
authors.lastname: Stambouli
authors.affiliation: Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC 20007 USA.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Islam
authors.affiliation: Laboratory of Genetics, Immunology and Human Pathology, Faculty of Sciences, University of Tunis El Manar, El Mannar I, Tunis, 2092 Tunisia.
authors.forename: Olfa
authors.initials: O
authors.lastname: Baroudi
authors.affiliation: Laboratory of Genetics, Immunology and Human Pathology, Faculty of Sciences, University of Tunis El Manar, El Mannar I, Tunis, 2092 Tunisia.
authors.forename: Bilel
authors.initials: B
authors.lastname: Neili
authors.affiliation: Department of Medical Oncology, Hannibal International Clinic, Les Berges du Lac 2, Tunis, Tunisia.
authors.forename: Farhat
authors.initials: F
authors.lastname: Benayed
authors.affiliation: Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC 20007 USA.
authors.forename: Simon
authors.initials: S
authors.lastname: Chivi
authors.affiliation: Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC 20007 USA.
authors.forename: Christopher
authors.initials: C
authors.lastname: Loffredo
authors.affiliation: Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC 20007 USA.
authors.forename: Irene A
authors.initials: IA
authors.lastname: Jillson
authors.affiliation: Laboratory of Genetics, Immunology and Human Pathology, Faculty of Sciences, University of Tunis El Manar, El Mannar I, Tunis, 2092 Tunisia.
authors.forename: Amel
authors.initials: A
authors.lastname: Benammar Elgaaied
authors.affiliation: Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC 20007 USA.
authors.forename: Jan K
authors.initials: JK
authors.lastname: Blancato
authors.affiliation: Laboratory of Genetics, Immunology and Human Pathology, Faculty of Sciences, University of Tunis El Manar, El Mannar I, Tunis, 2092 Tunisia.
authors.forename: Raja
authors.initials: R
authors.lastname: Marrakchi
keywords: Breast cancer; Circulating microRNA; Inflammatory breast cancer; Prognostic biomarkers; RT-qPCR; microRNA