title: In Vitro Protective Potentials of Annona muricata Leaf Extracts Against Sodium Arsenite-induced Toxicity.
abstract: Sodium arsenite (NaAsO2) is a metalloid which is present widely in the environment and its chronic exposure can contribute to the induction of oxidative stress, resulting in disturbances in various metabolic functions including liver cell death. Hence, there is a need to develop drugs from natural sources, which can reduce arsenic toxicity. While there have been reports regarding the antioxidant and protective potentials of Annona muricataleaf extracts, our study is the first ofits kind to extend these findings by specifically evaluating its ability to render protection against sodium arsenite (NaAsO2) induced toxicity (10 μM) in WRL-68 (human hepatic cells) and human erythrocytes by employing XTT and haemolysis inhibition assays respectively. The methanolic extract exhibited higher activity than the aqueous extract in both assays. The results showed a dose-dependent decrease in arsenic toxicity in both WRL-68 cells and erythrocytes, suggesting the protective nature of Annona muricatato mitigate arsenic toxicity. Hence the bioactive extracts can further be scrutinized for the identification and characterization of their principal contributors.
authors.forename: Vazhappilly Cijo
authors.initials: VC
authors.lastname: George
authors.forename: Devanga Ragupathi Naveen
authors.initials: DR
authors.lastname: Kumar
authors.forename: Palamadai Krishnan
authors.initials: PK
authors.lastname: Suresh
authors.affiliation: Department of Zoology, Government Arts College, Dharmapuri- 636705, Tamil Nadu, India. ashoku_2000@yahoo.com.
authors.forename: Rangasamy Ashok
authors.initials: RA
authors.lastname: Kumar
mesh_terms: D031041:Annona; D018053:Arsenites / Q000633:toxicity*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves; D020011:Protective Agents / Q000494:pharmacology*; D017670:Sodium Compounds / Q000633:toxicity*
chemical_list: D018053:Arsenites; D010936:Plant Extracts; D020011:Protective Agents; D017670:Sodium Compounds; C017947:sodium arsenite