title: [Effect of accutase dissociation and passage on the apoptosis of human striatum derived neural stem cells].
abstract: OBJECTIVE
  To explore the status of apoptosis in human striatum derived neural stem cells (NSCs) aft er accutase dissociation and passage.
  
  
  METHODS
  The NSCs were isolated from fetuses obtained through spontaneous abortion at 13- 18 weeks of pregnancy, which formed neurospheres in vitro. At passages of 3-5, the neurospheres were disassociated into single cell by accutase digestion and then passaged. At 1, 24 and 72 h after passage, the apoptosis of NSCs was measured by several methods, including active caspase-3 or TUNEL staining for fixed cells, Annexin V, Hoechst or PI staining for live cells.
  
  
  RESULTS
  At all of the 3 time points, the staining of TUNEL and active caspase-3 overlapped perfectly. The apoptosis rate of NSCs increased significantly from 20%-25% at 1 h to 75%-80% at 24 h after passage (P<0.01). At 72 h, the apoptosis rate was significantly decreased as compared to that at 24 h time point because of the self-renewal and proliferation of survived NSCs (P<0.01).
  
  
  CONCLUSIONS
  Many cells in the neurospheres formed by human striatum-derived NSCs underwent apoptosis soon after accutase disassociation. For NSCs cultured in vitro, anti-apoptosis treatments might be a good method to increase the self-renewal and the proliferation of NSCs.
authors.affiliation: Department of Physiology, Changzhi Medical College, Changzhi Shanxi 046000, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Li
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wang
authors.forename: Juan
authors.initials: J
authors.lastname: Song
authors.forename: Chen
authors.initials: C
authors.lastname: Li
authors.forename: Cuiying
authors.initials: C
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Zhao
authors.forename: Jinsheng
authors.initials: J
authors.lastname: Wang
mesh_terms: D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D002478:Cells, Cultured; D017364:Collagenases / Q000737:chemistry*; D003342:Corpus Striatum / Q000166:cytology; D005260:Female; D006801:Humans; D020287:In Situ Nick-End Labeling; D058953:Neural Stem Cells / Q000166:cytology*; D010447:Peptide Hydrolases / Q000737:chemistry*; D011247:Pregnancy
chemical_list: C575294:accutase; D010447:Peptide Hydrolases; C505407:CASP3 protein, human; D053148:Caspase 3; D017364:Collagenases