title: Microarrays in blood group genotyping.
abstract: Thirty-five blood group systems, containing more than 300 antigens, are listed by the International Society of Blood Transfusion (ISBT). Most of these antigens result from a single-nucleotide polymorphism (SNP). Blood group typing is conventionally carried out by serology. However, this technique has certain limitations and cannot respond to the growing demand for blood products typed for a large number of antigens. Here we describe a blood group genotyping assay, from genomic DNA extraction from whole-blood samples to results. After DNA extraction, the on-chip test is based on the hybridization of targets beforehand amplified by multiplex polymerase chain reaction, followed by a revelation step allowing the simultaneous identification of up to 24 blood group antigens and leading to the determination of extended genotypes.
authors.affiliation: AXO Science SAS, Villeurbanne, France.
authors.forename: Stephanie A
authors.initials: SA
authors.lastname: Boccoz
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Le Goff
authors.forename: Loïc J
authors.initials: LJ
authors.lastname: Blum
authors.forename: Christophe A
authors.initials: CA
authors.lastname: Marquette
mesh_terms: D001789:Blood Group Antigens / Q000235:genetics*; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D005838:Genotype; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*
chemical_list: D001789:Blood Group Antigens; D004247:DNA