title: Hypoglycemic Effect of the Methanol flower Extract of Piper Claussenianum and the Major Constituent 2',6'-dihydroxy-4'-methoxychalcone in Streptozotocin Diabetic Rats.
abstract: Piper claussenianum inflorescences crude methanol extract was tested for hypoglycemic effect in streptozotocin-induced diabetic rats. The blood glucose levels of rats treated with methanol extract were reduced from 318.4±28.1 mg/dl before treatment to 174.2±38.3 mg/dl after 12 days of treatment (P<0.05). Phytochemical studies were carried out on inflorescences methanol crude extract in order to investigate the possible metabolites responsible for the pharmacological properties of the extract. After chromatographic procedures, three flavonoids were isolated and characterized. The major compound 2',6'-dihydroxy-4'-methoxychalcone was also tested. Rats that received the chalcone content also displayed a reduction in blood glucose levels from 277.4±7.7 mg/dl before treatment to 158.8±9.2 mg/dl after 12 days of treatment (P<0.05). The results suggest this chalcone is one of the metabolite responsible for the blood glucose levels reduction in rats with streptozotocin-induced diabetes. The inflorescence crude extract of P. claussenianum was found to be composed mainly by flavonoids and may be a potential natural source of compounds with hypoglycemic properties.
authors.affiliation: Núcleo de Pesquisas de Produtos Naturais, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: A M
authors.initials: AM
authors.lastname: Marques
authors.affiliation: Programa de Desenvolvimento de Fármacos, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: S L
authors.initials: SL
authors.lastname: Pereira
authors.affiliation: Núcleo de Pesquisas de Produtos Naturais, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: R A
authors.initials: RA
authors.lastname: Paiva
authors.affiliation: Programa de Desenvolvimento de Fármacos, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: C V
authors.initials: CV
authors.lastname: Cavalcante
authors.affiliation: Escola de Medicina, Fundação Técnico Educacional Souza Marques, Rio de Janeiro, RJ, Brazil.
authors.forename: S Z
authors.initials: SZ
authors.lastname: Sudo
authors.affiliation: Núcleo de Pesquisas de Produtos Naturais, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: L W
authors.initials: LW
authors.lastname: Tinoco
authors.affiliation: Departamento de Produtos Naturais, Far-Manguinhos, Fiocruz, Rio de Janeiro, RJ, Brazil.
authors.forename: D L
authors.initials: DL
authors.lastname: Moreira
authors.affiliation: Instituto de Pesquisa Jardim Botânico do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: E F
authors.initials: EF
authors.lastname: Guimaraes
authors.affiliation: Programa de Desenvolvimento de Fármacos, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: R T
authors.initials: RT
authors.lastname: Sudo
authors.affiliation: Núcleo de Pesquisas de Produtos Naturais, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: M A C
authors.initials: MA
authors.lastname: Kaplan
authors.affiliation: Programa de Desenvolvimento de Fármacos, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: G Z
authors.initials: GZ
authors.lastname: Sudo
keywords: Piper claussenianum; chalcone; flavonoids; hypoglycemic effect