title: A method for simultaneous determination of 20 Fusarium toxins in cereals by high-resolution liquid chromatography-Orbitrap mass spectrometry with a pentafluorophenyl column.
abstract: A high-resolution liquid chromatography-Orbitrap mass spectrometry (LC-Orbitrap MS) method was developed for simultaneous determination of 20 Fusarium toxins (nivalenol, fusarenon-X, deoxynivalenol, 3-acetyl deoxynivalenol, 15-acetyl deoxynivalenol, HT-2 toxin, T-2 toxin, neosolaniol, diacetoxyscirpenol, fumonisin B1, fumonisin B2, fumonisin B3, fumonisin A1, fumonisin A2, fumonisin A3, zearalenone, α-zearalenol, β-zearalenol, α-zearalanol, and β-zearalanol) in cereals. The separation of 20 Fusarium toxins with good peak shapes was achieved using a pentafluorophenyl column, and Orbitrap MS was able to detect accurately from cereal matrix components within ±0.77 ppm. The samples were prepared using a QuEChERS kit for extraction and a multifunctional cartridge for purification. The linearity, repeatability, and recovery of the method were >0.9964, 0.8%-14.7%, and 71%-106%, respectively. Using this method, an analysis of 34 commercially available cereals detected the presence of deoxynivalenol, 15-acetyl deoxynivalenol, fumonisin B1, fumonisin B2, fumonisin B3, fumonisn A1, fumonisin A2, fumonisin A3, and zearalenone in corn samples with high concentration and frequency. Trichothecenes was detected from wheat samples with high frequency; in particular, the concentration of deoxynivalenol was high. Conversely, α-zearalenol, β-zearalenol, α-zearalanol, and β-zearalanol were not detected in any of the samples.
authors.affiliation: Research Laboratories for Food Safety Chemistry, Asahi Group Holdings, Ltd., 1-21, Midori 1-chome, Moriya-shi, Ibaraki 302-0106, Japan. masayoshi.tamura@asahigroup-holdings.com.|Graduate School of Medical Sciences, Kanazawa University, Kakuma-machi, Kanazawa-shi, Ishikawa 920-1192, Japan. masayoshi.tamura@asahigroup-holdings.com.
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Tamura
authors.affiliation: Research & Development Center, Asahi Group Holdings, Ltd., 1-21, Midori 1-chome, Moriya-shi, Ibaraki 302-0106, Japan. naoki.mochizuki@asahigroup-holdings.com.
authors.forename: Naoki
authors.initials: N
authors.lastname: Mochizuki
authors.affiliation: Research Laboratories for Food Safety Chemistry, Asahi Group Holdings, Ltd., 1-21, Midori 1-chome, Moriya-shi, Ibaraki 302-0106, Japan. yasushi.nagatomi@asahigroup-holdings.com.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Nagatomi
authors.affiliation: Research Laboratories for Food Safety Chemistry, Asahi Group Holdings, Ltd., 1-21, Midori 1-chome, Moriya-shi, Ibaraki 302-0106, Japan. koichi.harayama@asahigroup-holdings.com.
authors.forename: Koichi
authors.initials: K
authors.lastname: Harayama
authors.affiliation: Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kakuma-machi, Kanazawa-shi, Ishikawa 920-1192, Japan. toriba@p.kanazawa-u.ac.jp.
authors.forename: Akira
authors.initials: A
authors.lastname: Toriba
authors.affiliation: Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kakuma-machi, Kanazawa-shi, Ishikawa 920-1192, Japan. hayakawa@p.kanazawa-u.ac.jp.
authors.forename: Kazuichi
authors.initials: K
authors.lastname: Hayakawa
mesh_terms: D002853:Chromatography, Liquid; D002523:Edible Grain / Q000737:chemistry*; D004784:Environmental Monitoring; D005506:Food Contamination / Q000032:analysis; D005670:Fusarium; D001467:Hordeum; D007564:Japan; D013058:Mass Spectrometry; D009183:Mycotoxins / Q000032:analysis*; D014908:Triticum; D003313:Zea mays
chemical_list: D009183:Mycotoxins
keywords: Fusarium toxins; LC-Orbitrap MS; cereals; pentafluorophenyl column; simultaneous determination