title: Capping protein integrates multiple MAMP signalling pathways to modulate actin dynamics during plant innate immunity.
abstract: Plants and animals perceive diverse microbe-associated molecular patterns (MAMPs) via pattern recognition receptors and activate innate immune signalling. The actin cytoskeleton has been suggested as a target for innate immune signalling and a key transducer of cellular responses. However, the molecular mechanisms underlying actin remodelling and the precise functions of these rearrangements during innate immunity remain largely unknown. Here we demonstrate rapid actin remodelling in response to several distinct MAMP signalling pathways in plant epidermal cells. The regulation of actin dynamics is a convergence point for basal defence machinery, such as cell wall fortification and transcriptional reprogramming. Our quantitative analyses of actin dynamics and genetic studies reveal that MAMP-stimulated actin remodelling is due to the inhibition of capping protein (CP) by the signalling lipid, phosphatidic acid. In addition, CP promotes resistance against bacterial and fungal phytopathogens. These findings demonstrate that CP is a central target for the plant innate immune response.
authors.affiliation: Department of Biological Sciences, Purdue University, 335 Hansen Life Sciences Building, West Lafayette, Indiana 47907-2064, USA.
authors.forename: Jiejie
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Biological Sciences, Purdue University, 335 Hansen Life Sciences Building, West Lafayette, Indiana 47907-2064, USA.
authors.forename: Jessica L
authors.initials: JL
authors.lastname: Henty-Ridilla
authors.affiliation: Department of Biological Sciences, Purdue University, 335 Hansen Life Sciences Building, West Lafayette, Indiana 47907-2064, USA.
authors.forename: Benjamin H
authors.initials: BH
authors.lastname: Staiger
authors.affiliation: Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, Michigan 48824-6254, USA.
authors.forename: Brad
authors.initials: B
authors.lastname: Day
authors.affiliation: 1] Department of Biological Sciences, Purdue University, 335 Hansen Life Sciences Building, West Lafayette, Indiana 47907-2064, USA [2] The Bindley Bioscience Center, Discovery Park, Purdue University, 1203 West State Street, West Lafayette, Indiana 47907, USA.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Staiger
mesh_terms: D051344:Actin Capping Proteins / Q000276:immunology*; D008841:Actin Cytoskeleton / Q000276:immunology*; D000528:Alternaria / Q000276:immunology; D060487:Alternariosis / Q000276:immunology*; D017360:Arabidopsis / Q000276:immunology*; D007113:Immunity, Innate / Q000276:immunology*; D019441:Plant Epidermis / Q000166:cytology / Q000276:immunology*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D051344:Actin Capping Proteins