title: Characterization of a novel β-cypermethrin-degrading Aspergillus niger YAT strain and the biochemical degradation pathway of β-cypermethrin.
abstract: Aspergillus niger YAT strain was obtained from Chinese brick tea (Collection number: CGMCC 10,568) and identified on the basis of morphological characteristics and internal transcribed spacer (ITS) sequence. The strain could degrade 54.83 % of β-cypermethrin (β-CY; 50 mg L(-1)) in 7 days and 100 % of 3-phenoxybenzoic acid (3-PBA; 100 mg L(-1)) in 22 h. The half-lives of β-CY and 3-PBA range from 3.573 to 11.748 days and from 5.635 to 12.160 h, respectively. The degradation of β-CY and 3-PBA was further described using first-order kinetic models. The pathway and mechanism of β-CY degraded by YAT were investigated by analyzing the degraded metabolites through high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS). Relevant enzymatic activities and substrate utilization were also investigated. β-CY degradation products were analyzed. Results indicated that YAT strain transformed β-CY into 3-PBA. 3-PBA was then gradually transformed into permethric acid, protocatechuic acid, 3-hydroxy-5-phenoxy benzoic acid, gallic acid, and phenol gradually. The YAT strain can also effectively degrade these metabolites. The results indicated that YAT strain has potential applications in bioremediation of pyrethroid insecticide (PI)-contaminated environments and fermented food.
authors.affiliation: College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan, 625014, People's Republic of China.
authors.forename: Weiqin
authors.initials: W
authors.lastname: Deng
authors.forename: Derong
authors.initials: D
authors.lastname: Lin
authors.forename: Kai
authors.initials: K
authors.lastname: Yao
authors.forename: Huaiyu
authors.initials: H
authors.lastname: Yuan
authors.forename: Zhilong
authors.initials: Z
authors.lastname: Wang
authors.forename: Jianlong
authors.initials: J
authors.lastname: Li
authors.forename: Likou
authors.initials: L
authors.lastname: Zou
authors.forename: Xinfeng
authors.initials: X
authors.lastname: Han
authors.forename: Kang
authors.initials: K
authors.lastname: Zhou
authors.forename: Li
authors.initials: L
authors.lastname: He
authors.forename: Xinjie
authors.initials: X
authors.lastname: Hu
authors.forename: Shuliang
authors.initials: S
authors.lastname: Liu
mesh_terms: D001234:Aspergillus niger / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002681:China; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D011722:Pyrethrins / Q000737:chemistry / Q000378:metabolism*; D012989:Soil Pollutants / Q000737:chemistry / Q000378:metabolism; D045730:Soy Foods / Q000382:microbiology; D013662:Tea / Q000382:microbiology; D014920:Wine / Q000382:microbiology
chemical_list: D005656:Fungal Proteins; D011722:Pyrethrins; D012989:Soil Pollutants; D013662:Tea; C017160:cypermethrin