title: Lysobacter tyrosinelyticus sp. nov. isolated from Gyeryongsan national park soil.
abstract: A novel Gram-negative, rod-shaped (0.2-0.5 μm × 1.5-2.5 μm), aerobic, non-motile bacterium was isolated from Gyeryongsan national park soil, Republic of Korea. The novel isolate was designated as THG-DN8.2(T). The strain grows optimally at 28 °C, at pH 7 and in the absence of NaCl. Phylogenetic analysis based on 16S rRNA gene sequence showed that the novel isolate shared the highest sequence similarity with Lysobacter oryzae KCTC 22249(T) followed by Lysobacter yangpyeongensis KACC 11407(T) and Lysobacter niabensis KACC 11587(T). The DNA G+C content of strain THG-DN8.2T is 66.0 mol% and ubiquinone Q-8 is the main isoprenoid quinone. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and phosphatidyl-N-methylethanolamine. The major fatty acids of strain THG-DN8.2(T) were identified as iso-C15:0, iso-C16:0, and C16:1 ω7с alcohol. The phylogenetic distinctiveness and phenotypic characteristics differentiated strain THG-DN8.2(T) from closely related Lysobacter species. The results of polyphasic taxonomic analysis suggest that strain THG-DN8.2(T) represents a novel species of the genus Lysobacter, for which the name Lysobacter tyrosinelyticus sp. nov. is proposed. The type strain is THG-DN8.2(T) (= KCTC 42235(T) = JCM 30320(T)).
authors.affiliation: Department of Oriental Medicine Biotechnology, College of Life Science, Kyung Hee University Global Campus, Gyeonggi-do, 446-701, Republic of Korea.
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.forename: Hina
authors.initials: H
authors.lastname: Singh
authors.forename: Hien T T
authors.initials: HT
authors.lastname: Ngo
authors.forename: KyungHwa
authors.initials: K
authors.lastname: Won
authors.forename: Ki-Young
authors.initials: KY
authors.lastname: Kim
authors.forename: Tae-Hoo
authors.initials: TH
authors.lastname: Yi
mesh_terms: D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D053528:Lysobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008969:Molecular Sequence Data; D000068316:Parks, Recreational; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D012988:Soil Microbiology; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride