title: Silica-based 2-(N,N-dimethylamino)-1,3-propanediol hydrophilic interaction liquid chromatography stationary phase for separating cephalosporins and carbapenems.
abstract: A silica-based stationary phase bearing both hydrophilic hydroxyl and amino groups was developed by covalently bonding a small molecular N,N-dimethylamino 1,3-propanediol moiety onto silica beads via copper(I)-catalyzed Huisgen azide-alkyne 1,3-dipolar cycloaddition (CuAAC). This new stationary phase showed good HILIC characteristics and high column efficiency (the theoretical plate number is up to 37000 plates m(-1) in the case of inosine) in the separation of polar compounds, such as nucleosides and bases, organic acids, cephalosporins, and carbapenems.
authors.affiliation: School of Pharmacy, Shanghai Key Laboratory of New Drug Design, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yin
authors.forename: Lingping
authors.initials: L
authors.lastname: Cheng
authors.forename: Huihui
authors.initials: H
authors.lastname: Chai
authors.forename: Ruiqiang
authors.initials: R
authors.lastname: Guo
authors.forename: Renhua
authors.initials: R
authors.lastname: Liu
authors.forename: Changhu
authors.initials: C
authors.lastname: Chu
authors.forename: John A
authors.initials: JA
authors.lastname: Palasota
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Cai
mesh_terms: D000480:Alkynes / Q000737:chemistry; D001386:Azides / Q000737:chemistry; D015780:Carbapenems / Q000302:isolation & purification*; D002511:Cephalosporins / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D061565:Cycloaddition Reaction; D011409:Propylene Glycols / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D000480:Alkynes; D001386:Azides; D015780:Carbapenems; D002511:Cephalosporins; D011409:Propylene Glycols; C041787:1,3-propanediol; D012822:Silicon Dioxide