title: The proportion of CD16(+)CD14(dim) monocytes increases with tumor cell load in bone marrow of patients with multiple myeloma.
abstract: Multiple myeloma is an incurable cancer with expansion of malignant plasma cells in the bone marrow. Previous studies have shown that monocytes and macrophages in the bone marrow milieu are important for tumor growth and may play a role in the drug response. We therefore characterized monocytes in bone marrow aspirates by flow cytometry. We found that there was significant correlation between the proportion of CX3CR1 (+), CD16(+)CD14(dim) non classical monocytes, and percent plasma cells (PC) in the bone marrow of myeloma patients. The bone marrow monocytes could be stimulated by TLR ligands to produce cytokines which promote myeloma cell growth. The proportion of the non-classical monocytes increased with the tumor load, particularly in patients with tumor loads in the range of 10-30% bone marrow PC.
authors.affiliation: K.G. Jebsen Center for Myeloma Research Trondheim, Norway.
authors.forename: Anne M
authors.initials: AM
authors.lastname: Sponaas
authors.affiliation: K.G. Jebsen Center for Myeloma Research Trondheim, Norway ; Department for Cancer Research and Molecular Medicine, Center for Molecular Inflammation Research NTNU, Trondheim, Norway.
authors.forename: Siv H
authors.initials: SH
authors.lastname: Moen
authors.affiliation: K.G. Jebsen Center for Myeloma Research Trondheim, Norway.
authors.forename: Nina B
authors.initials: NB
authors.lastname: Liabakk
authors.affiliation: K.G. Jebsen Center for Myeloma Research Trondheim, Norway ; Section of Hematology, St. Olav's University Hospital Trondheim, Norway.
authors.forename: Emadoldin
authors.initials: E
authors.lastname: Feyzi
authors.affiliation: K.G. Jebsen Center for Myeloma Research Trondheim, Norway.
authors.forename: Toril
authors.initials: T
authors.lastname: Holien
authors.affiliation: K.G. Jebsen Center for Myeloma Research Trondheim, Norway.
authors.forename: Solveig
authors.initials: S
authors.lastname: Kvam
authors.affiliation: K.G. Jebsen Center for Myeloma Research Trondheim, Norway ; Section of Hematology, St. Olav's University Hospital Trondheim, Norway.
authors.forename: Lill Anny G
authors.initials: LA
authors.lastname: Grøseth
authors.affiliation: K.G. Jebsen Center for Myeloma Research Trondheim, Norway.
authors.forename: Berit
authors.initials: B
authors.lastname: Størdal
authors.affiliation: K.G. Jebsen Center for Myeloma Research Trondheim, Norway.
authors.forename: Glenn
authors.initials: G
authors.lastname: Buene
authors.affiliation: K.G. Jebsen Center for Myeloma Research Trondheim, Norway ; Department for Cancer Research and Molecular Medicine, Center for Molecular Inflammation Research NTNU, Trondheim, Norway.
authors.forename: Terje
authors.initials: T
authors.lastname: Espevik
authors.affiliation: K.G. Jebsen Center for Myeloma Research Trondheim, Norway ; Section of Hematology, St. Olav's University Hospital Trondheim, Norway.
authors.forename: Anders
authors.initials: A
authors.lastname: Waage
authors.affiliation: K.G. Jebsen Center for Myeloma Research Trondheim, Norway ; Department for Cancer Research and Molecular Medicine, Center for Molecular Inflammation Research NTNU, Trondheim, Norway.
authors.forename: Therese
authors.initials: T
authors.lastname: Standal
authors.affiliation: K.G. Jebsen Center for Myeloma Research Trondheim, Norway ; Department for Cancer Research and Molecular Medicine, Center for Molecular Inflammation Research NTNU, Trondheim, Norway.
authors.forename: Anders
authors.initials: A
authors.lastname: Sundan
keywords: Apoptosis; bone marrow; monocytes; multiple myeloma