title: Bioactive compounds isolated from submerged fermentations of the Chilean fungus Stereum rameale.
abstract: Liquid fermentations of the fungus Stereum rameale (N° 2511) yielded extracts with antibacterial activity. The antibacterial activity reached its peak after 216 h of stirring. Bioassay-guided fractionation methods were employed for the isolation of the bioactive metabolites. Three known compounds were identified: MS-3 (1), vibralactone (2) and vibralactone B (3). The three compounds showed antibacterial activity as a function of their concentration. Minimal bactericidal concentrations (MBC) of compound 1 against Gram-positive bacteria were as follows: Bacillus cereus (50 μg/mL), Bacillus subtilis (10 μg/mL) and Staphylococcus aureus (100 μg/mL). Compounds 2 and 3 were active only against Gram-negative bacteria. The MBC of compound 2 against Escherichia coli was 200 μg/mL. Compound 3 inhibited significantly the growth of E. coli and Pseudomonas aeruginosa, with MBC values of 50 and 100 μg/mL, respectively.
authors.forename: Pedro
authors.initials: P
authors.lastname: Aqueveque
authors.forename: Carlos Leonardo
authors.initials: CL
authors.lastname: Céspedes
authors.forename: José
authors.initials: J
authors.lastname: Becerra
authors.forename: Marcelo
authors.initials: M
authors.lastname: Dávila
authors.forename: Olov
authors.initials: O
authors.lastname: Sterner
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects; D002677:Chile; D004926:Escherichia coli / Q000187:drug effects; D005285:Fermentation; D005658:Fungi / Q000737:chemistry* / Q000254:growth & development; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents