title: Self-Assembly of Fluorescent Hybrid Core-Shell Nanoparticles and Their Application.
abstract: In this work, a fluorescent hybrid core-shell nanoparticle was prepared by coating a functional polymer shell onto silver nanoparticles via a facile one-pot method. The biomolecule poly-L-lysine (PLL) was chosen as the polymer shell and assembled onto the silver core via the amine-reactive cross-linker, 3,3'-dithiobis(sulfosuccinimidylpropionate). The fluorescent anticancer drug, doxorubicin, was incorporated into the PLL shell through the same linkage. As the cross-linker possesses a thiol-cleavable disulfide bond, disassembly of the PLL shell was observed in the presence of glutathione, leading to controllable doxorubicin release. The silver core there provided an easily modified surface to facilitate the shell coating and ensures the efficient separation of as-prepared nanoparticles from their reaction mixture through centrifugation. Cell assays show that the prepared hybrid fluorescent nanoparticles can internalize into cells possessing excellent biocompatibility prior to the release of doxorubicin, terminating cancer cells efficiently as the doxorubicin is released at the intracellular glutathione level. Such properties are important for designing smart containers for target drug delivery and cellular imaging.
authors.affiliation: School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing 100083, P. R. China.
authors.forename: Chun
authors.initials: C
authors.lastname: Wang
authors.affiliation: School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing 100083, P. R. China.
authors.forename: Fu
authors.initials: F
authors.lastname: Tang
authors.affiliation: School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing 100083, P. R. China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing 100083, P. R. China.
authors.forename: Lidong
authors.initials: L
authors.lastname: Li
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004317:Doxorubicin / Q000737:chemistry / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D011107:Polylysine / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D005456:Fluorescent Dyes; D011107:Polylysine; D004317:Doxorubicin
keywords: cellular imaging; core−shell nanoparticles; disassembly; fluorescence; polymer; self-assembly