title: Overexpression of PLAP-1 in bone marrow stromal cells inhibits the rat critical-size skull defect repair.
abstract: Periodontal ligament-associated protein-1 (PLAP-1) is an osteogenisis negative regulatory gene that inhibits the differentiation of rat bone marrow stromal cells (rBMSCs) into osteoblasts in vitro. The aim of this study was to verify whether PLAP-1 acted as an inhibitory factor of rBMSCs in rat critical-size skull defect repair in vivo. Six-week old male Wistar rats treated with a drill-hole injury were randomly assigned into five groups PLAP-1-transfected rBMSC group, empty vector-transfected rBMSC group, normal rBMSC group, collagen group and blank control group according to the treatment factors. Skull specimens were obtained at 8 weeks after surgery. X-ray examination, histological observation of hard tissue slices (HE, Masson staining and von Kossa staining), immunohistochemical staining were applied to evaluate the repair effects. X-ray examination showed that repair effect of the PLAP-1-transfected rBMSC group was significantly worse than that of the empty vector-transfected rBMSC group and normal rBMSC group. In HE staining, regenerated bone could only be observed in the partial defect area of the PLAP-1-transfected rBMSC group. However, new bone filled nearly the entire defects in the empty vector-transfected rBMSC group and normal rBMSC group. Mineralization of new bone in the two groups was significantly higher than that of the PLAP-1-transfected rBMSC group, according to Masson and von Kossa staining. Meanwhile, the transfected PLAP-1 gene worked well in vivo. Positive expression of PLAP-1 protein was only distributed in the newly formed bone of the PLAP-1-transfected rBMSC group, no positive staining was observed in the other four groups. Overexpression of PLAP-1 in rBMSCs inhibits new bone formation and mineralization in rat critical-size skull defects in vivo.
authors.affiliation: Shandong Provincial Key Laboratory of Oral Biomedicine, Department of Periodontology, School and Hospital of Stomatology, Shandong University, 44-1# West Wenhua Road, Jinan, 250012, Shandong, People's Republic of China.
authors.forename: Xijiao
authors.initials: X
authors.lastname: Yu
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.forename: Ying
authors.initials: Y
authors.lastname: Hu
authors.forename: Yan
authors.initials: Y
authors.lastname: Gao
authors.forename: Changjie
authors.initials: C
authors.lastname: Xiao
authors.forename: Shuang
authors.initials: S
authors.lastname: Liu
authors.forename: Shu
authors.initials: S
authors.lastname: Li
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D002478:Cells, Cultured; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D061251:Primary Cell Culture; D011859:Radiography; D017208:Rats, Wistar; D012886:Skull / Q000000981:diagnostic imaging / Q000473:pathology
chemical_list: C000594598:Aspn protein, rat; D016326:Extracellular Matrix Proteins