title: [Correlation between biochemical parameters of oxidative stress, endogenous intoxication and regulation of vascular tone in patients with burn injury].
abstract: Burn injury is accompanied by the formation of reactive oxygen species (ROS). Excessive production of ROS results in oxidative stress. Peroxidation damage of proteins causes their degradation and the formation of toxic fragments con- tributing to the development of endogenous intoxication. Furthermore, burns cause pronounced inflammatory reaction in the lesion site leading to poor circulation. The purpose of this study was an investigation of relationship between disturbances in the prooxidant/antioxidant system, severity of endogenous intoxication and disturbances of endogenous vascular regulation to assess the severity and prognosis of complications in patients with burn injury. 26 patients with- burn injury were investigated; they were divided into 2 groups according to the severity of injury on the basis of Frank index (FI): group 1--FI < 60 CU and group 2--FI ≥ 60 CU. The investigation of blood serum was performed on 1-3, 7, 14, 21 and 28 day after burn injury. Malondialdehyde (MDA), total antioxidant status (TAS), the level of middle weight molecules, stable metabolites of nitric oxide (NOx) and angiotensin-converting enzyme (ACE) activity were determined in the serum. Significant increase of MDA level, decrease of TAS and NOx level were found in two groups of patients throughout the observation period. We also found a disturbance in coupled interaction of NO and ACE. These data point to the development of oxidative stress and imbalance in endogenous regulation of vascular tone. There was a trend toward more pronounced oxidative stress in group 2. Significant correlations between parameters of oxidative stress, endogenous intoxication, endogenous factors of vascular regulation, depth of burn injury and FI were obtained in two groups. MDA, TAS can serve as one of the prognostic markers of condition severity of burned patients and therapy adequacy.
authors.forename: E V
authors.initials: EV
authors.lastname: Klychnikova
authors.forename: E V
authors.initials: EV
authors.lastname: Tazina
authors.forename: S V
authors.initials: SV
authors.lastname: Smirnov
authors.forename: T G
authors.initials: TG
authors.lastname: Spiridonova
authors.forename: E A
authors.initials: EA
authors.lastname: Zhirkova
authors.forename: V S
authors.initials: VS
authors.lastname: Borisov
authors.forename: M A
authors.initials: MA
authors.lastname: Godkov
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood; D002056:Burns / Q000097:blood* / Q000503:physiopathology; D016022:Case-Control Studies; D003627:Data Interpretation, Statistical; D005260:Female; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D008833:Microcirculation / Q000502:physiology*; D008875:Middle Aged; D009569:Nitric Oxide / Q000097:blood*; D018384:Oxidative Stress; D007703:Peptidyl-Dipeptidase A / Q000097:blood; D015599:Trauma Severity Indices
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D009569:Nitric Oxide; D008315:Malondialdehyde; D007703:Peptidyl-Dipeptidase A