title: Development and Evaluation of Novel Real-Time Reverse Transcription-PCR Assays with Locked Nucleic Acid Probes Targeting Leader Sequences of Human-Pathogenic Coronaviruses.
abstract: Based on findings in small RNA-sequencing (Seq) data analysis, we developed highly sensitive and specific real-time reverse transcription (RT)-PCR assays with locked nucleic acid probes targeting the abundantly expressed leader sequences of Middle East respiratory syndrome coronavirus (MERS-CoV) and other human coronaviruses. Analytical and clinical evaluations showed their noninferiority to a commercial multiplex PCR test for the detection of these coronaviruses.
authors.affiliation: State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China.
authors.forename: Jasper Fuk-Woo
authors.initials: JF
authors.lastname: Chan
authors.affiliation: Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.
authors.forename: Garnet Kwan-Yue
authors.initials: GK
authors.lastname: Choi
authors.affiliation: Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.
authors.forename: Alan Ka-Lun
authors.initials: AK
authors.lastname: Tsang
authors.affiliation: Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.
authors.forename: Kah-Meng
authors.initials: KM
authors.lastname: Tee
authors.affiliation: Department of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong Special Administrative Region, China.
authors.forename: Ho-Yin
authors.initials: HY
authors.lastname: Lam
authors.affiliation: Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.
authors.forename: Cyril Chik-Yan
authors.initials: CC
authors.lastname: Yip
authors.affiliation: State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China.
authors.forename: Kelvin Kai-Wang
authors.initials: KK
authors.lastname: To
authors.affiliation: Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.
authors.forename: Vincent Chi-Chung
authors.initials: VC
authors.lastname: Cheng
authors.affiliation: Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.
authors.forename: Man-Lung
authors.initials: ML
authors.lastname: Yeung
authors.affiliation: State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China.
authors.forename: Susanna Kar-Pui
authors.initials: SK
authors.lastname: Lau
authors.affiliation: State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China.
authors.forename: Patrick Chiu-Yat
authors.initials: PC
authors.lastname: Woo
authors.affiliation: Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.
authors.forename: Kwok-Hung
authors.initials: KH
authors.lastname: Chan
authors.affiliation: Department of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong Special Administrative Region, China.
authors.forename: Bone Siu-Fai
authors.initials: BS
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China kyyuen@hku.hk.
authors.forename: Kwok-Yung
authors.initials: KY
authors.lastname: Yuen
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D002648:Child; D002675:Child, Preschool; D017934:Coronavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D018352:Coronavirus Infections / Q000175:diagnosis* / Q000821:virology; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D015345:Oligonucleotide Probes / Q000235:genetics*; D009841:Oligonucleotides / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D015345:Oligonucleotide Probes; D009841:Oligonucleotides; C477371:locked nucleic acid