title: Comparisons of the pharmacokinetic profile of four bioactive components after oral administration of gan-sui-ban-xia decoction plus-minus gansui and gancao drug combination in normal rats.
abstract: Gan-Sui-Ban-Xia Decoction (GSBXD) was first presented by Zhang Zhongjing in the book Synopsis of Golden Chamber during the Han Dynasty period. The formula was then used for the treatment of persistent fluid retention with floating pulse in Traditional Chinese Medicine (TCM), which in modern medicine is known as malignant ascites. Here, a rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed for the determination of glycyrrhizinic acid, liquiritin, paeoniflorin, albiflorin after oral administration of GSBXD plus-minus Gansui and Gancao anti-drug combination to investigate the possible pharmacokinetic profile differences of different prescriptions with GSBXD in normal rats. The differences of pharmacokinetic parameters among groups were tested by the Student's t-test with p < 0.05 as the level of significance. Significant differences were found between the Gansui and Gancao anti-drug combination and other herbs in GSBXD on pharmacokinetic profile of glycyrrhizinic acid, liquiritin, paeoniflorin and albiflorin. The obtained knowledge might contribute to the rationality of the clinic use of GSBXD and also reveal the compatibility conditions of the Gansui and Gancao anti-drug combination.
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. zy91923@163.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. qiandwnj@126.com.
authors.forename: Dawei
authors.initials: D
authors.lastname: Qian
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. ptianxie@126.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. 04040416@163.com.
authors.forename: Zhenghua
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. hjsky2010@hotmail.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Huang
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. jzd666@163.com.
authors.forename: Junzuan
authors.initials: J
authors.lastname: Xi
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. njuguo@njutcm.edu.cn.
authors.forename: Jianming
authors.initials: J
authors.lastname: Guo
authors.affiliation: The No.1 Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210023, China. zxp@njucm.edu.cn.
authors.forename: Xueping
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Basic Medical College, Beijing University of Chinese Medicine, Beijing 100092, China. Zhonggansheng@sohu.com.
authors.forename: Gansheng
authors.initials: G
authors.lastname: Zhong
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. dja@njutcm.edu.cn.
authors.forename: Jinao
authors.initials: J
authors.lastname: Duan
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001201:Ascites / Q000188:drug therapy; D001952:Bridged-Ring Compounds / Q000097:blood / Q000493:pharmacokinetics*; D002853:Chromatography, Liquid; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics*; D028481:Euphorbia / Q000378:metabolism; D044950:Flavanones / Q000097:blood / Q000493:pharmacokinetics*; D005960:Glucosides / Q000097:blood / Q000493:pharmacokinetics*; D029882:Glycyrrhiza uralensis / Q000378:metabolism; D019695:Glycyrrhizic Acid / Q000097:blood / Q000493:pharmacokinetics*; D008297:Male; D008516:Medicine, Chinese Traditional; D039821:Monoterpenes / Q000097:blood / Q000493:pharmacokinetics*; D029596:Paeonia / Q000378:metabolism; D030021:Pinellia / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D053719:Tandem Mass Spectrometry; D006027:Glycosides
chemical_list: D001952:Bridged-Ring Compounds; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005960:Glucosides; D019695:Glycyrrhizic Acid; D039821:Monoterpenes; C015423:peoniflorin; D006027:Glycosides; C014959:albiflorin; C512196:liquiritin
keywords: Gan-Sui-Ban-Xia Decoction; Gansui and Gancao anti-drug combination; UPLC–TQ/MS; pharmacokinetics influence