title: Glucocorticoid receptor is involved in the breed-dependent transcriptional regulation of 3β-hydroxysteroid dehydrogenase in the liver of preweaning piglets.
abstract: BACKGROUND
  Hepatic 3β-hydroxysteroid dehydrogenase (3β-HSD) plays an important role in steroid inactivation and catabolism. Serum concentrations of steroid hormones differ significantly between breeds in pigs, however the molecular mechanism regulating hepatic 3β-HSD expression in different breeds of pigs is poorly understood. In the present study, we used preweaning purebred male Large White (LW) and Erhualian (EHL) piglets as model to investigate the breed difference in the expression and regulation of 3β-HSD gene in porcine liver.
  
  
  RESULTS
  The hepatic expression of 3β-HSD mRNA was significantly lower (P < 0.01) in EHL piglets compared to that in LW piglets. Significant breed differences were detected for the hepatic expression of transcription factors such as androgen receptor (AR), glucocorticoid receptor (GR), and CCAAT/enhancer binding protein β (C/EBPβ). The nucleoprotein contents of AR (P < 0.05), GR (P < 0.01) and phospho-Ser(211)GR (P < 0.01) were significantly higher in the liver of EHL piglets. Chromatin immunoprecipitation (ChIP) assay demonstrated significantly lower binding of GR, but not AR or C/EBPβ, to 3β-HSD gene promoter in EHL piglets (P < 0.05). GR was not detected to interact with C/EBPβ or AR in the co-immunoprecipitation analysis.
  
  
  CONCLUSIONS
  These results indicate that GR binding to 3β-HSD promoter is involved in the breed-dependent 3β-HSD expression in the liver of piglets.
authors.affiliation: Key Laboratory of Animal Physiology and Biochemistry, Nanjing Agricultural University, Nanjing, 210095, P. R. China. lixianlovelv@163.com.
authors.forename: Xian
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Animal Physiology and Biochemistry, Nanjing Agricultural University, Nanjing, 210095, P. R. China. jymrobin@hotmail.com.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Jia
authors.affiliation: Key Laboratory of Animal Physiology and Biochemistry, Nanjing Agricultural University, Nanjing, 210095, P. R. China. ysxlrs@163.com.
authors.forename: Runsheng
authors.initials: R
authors.lastname: Li
authors.affiliation: Key Laboratory of Animal Physiology and Biochemistry, Nanjing Agricultural University, Nanjing, 210095, P. R. China. sunzhiyuan1984@163.com.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Key Laboratory of Animal Physiology and Biochemistry, Nanjing Agricultural University, Nanjing, 210095, P. R. China. 243397182@163.com.
authors.forename: Xi
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Animal Physiology and Biochemistry, Nanjing Agricultural University, Nanjing, 210095, P. R. China. suishiyan2005@aliyun.com.
authors.forename: Shiyan
authors.initials: S
authors.lastname: Sui
authors.affiliation: Key Laboratory of Animal Physiology and Biochemistry, Nanjing Agricultural University, Nanjing, 210095, P. R. China. zhao.ruqian@gmail.com.
authors.forename: Ruqian
authors.initials: R
authors.lastname: Zhao
mesh_terms: D015096:3-Hydroxysteroid Dehydrogenases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000235:genetics / Q000378:metabolism; D002843:Chromatin; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D019751:Intramolecular Transferases / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008297:Male; D011944:Receptors, Androgen / Q000235:genetics / Q000378:metabolism; D011965:Receptors, Glucocorticoid / Q000235:genetics / Q000378:metabolism*; D013552:Swine / Q000235:genetics*; D014157:Transcription Factors
chemical_list: D029681:Arabidopsis Proteins; D022782:CCAAT-Enhancer-Binding Protein-beta; D002843:Chromatin; D011944:Receptors, Androgen; D011965:Receptors, Glucocorticoid; D014157:Transcription Factors; D015096:3-Hydroxysteroid Dehydrogenases; D019751:Intramolecular Transferases; C568454:marneral synthase, Arabidopsis