title: Size Control of (99m)Tc-tin Colloid Using PVP and Buffer Solution for Sentinel Lymph Node Detection.
abstract: Colloidal particle size is an important characteristic that allows mapping sentinel nodes in lymphoscintigraphy. This investigation aimed to introduce different ways of making a (99m)Tc-tin colloid with a size of tens of nanometers. All agents, tin fluoride, sodium fluoride, poloxamer-188, and polyvinylpyrrolidone (PVP), were mixed and labeled with (99m)Tc. Either phosphate or sodium bicarbonate buffers were used to adjust the pH levels. When the buffers were added, the size of the colloids increased. However, as the PVP continued to increase, the size of the colloids was controlled to within tens of nanometers. In all samples, phosphate buffer added PVP (30 mg) stabilized tin colloid ((99m)Tc-PPTC-30) and sodium bicarbonate solution added PVP (50 mg) stabilized tin colloid ((99m)Tc-BPTC-50) were chosen for in vitro and in vivo studies. (99m)Tc-BPTC-50 (<20 nm) was primarily located in bone marrow and was then secreted through the kidneys, and (99m)Tc-PPTC-30 (>100 nm) mainly accumulated in the liver. When a rabbit was given a toe injection, the node uptake of (99m)Tc-PPTC-30 decreased over time, while (99m)Tc-BPTC-50 increased. Therefore, (99m)Tc-BPTC-50 could be a good candidate radiopharmaceutical for sentinel node detection. The significance of this study is that nano-sized tin colloid can be made very easily and quickly by PVP.
authors.affiliation: Department of Nuclear Medicine, Molecular Imaging & Therapeutic Medicine Research Center, Cyclotron Research Center, Institute for Medical Science, Biomedical Research Institute, Chonbuk National University Medical School and Hospital, Jeonju, Korea.
authors.forename: Eun-Mi
authors.identifier: 0000-0001-6222-3486
authors.initials: EM
authors.lastname: Kim
authors.affiliation: Department of Nuclear Medicine, Molecular Imaging & Therapeutic Medicine Research Center, Cyclotron Research Center, Institute for Medical Science, Biomedical Research Institute, Chonbuk National University Medical School and Hospital, Jeonju, Korea.
authors.forename: Seok Tae
authors.identifier: 0000-0002-3407-7212
authors.initials: ST
authors.lastname: Lim
authors.affiliation: Department of Nuclear Medicine, Molecular Imaging & Therapeutic Medicine Research Center, Cyclotron Research Center, Institute for Medical Science, Biomedical Research Institute, Chonbuk National University Medical School and Hospital, Jeonju, Korea.
authors.forename: Myung-Hee
authors.identifier: 0000-0002-7955-721X
authors.initials: MH
authors.lastname: Sohn
authors.affiliation: Department of Nuclear Medicine, Molecular Imaging & Therapeutic Medicine Research Center, Cyclotron Research Center, Institute for Medical Science, Biomedical Research Institute, Chonbuk National University Medical School and Hospital, Jeonju, Korea.
authors.forename: Hwan-Jeong
authors.identifier: 0000-0002-9539-342X
authors.initials: HJ
authors.lastname: Jeong
mesh_terms: D000818:Animals; D002021:Buffers; D045744:Cell Line, Tumor; D006801:Humans; D008198:Lymph Nodes / Q000000981:diagnostic imaging*; D008207:Lymphatic Metastasis; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D051379:Mice; D009374:Neoplasms, Experimental / Q000000981:diagnostic imaging*; D010316:Particle Size; D011205:Povidone / Q000737:chemistry*; D011817:Rabbits; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000138:chemical synthesis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017556:Technetium Compounds / Q000737:chemistry*; D014001:Tin / Q000737:chemistry*; D017971:Tin Compounds / Q000737:chemistry*
chemical_list: D002021:Buffers; D019275:Radiopharmaceuticals; D017556:Technetium Compounds; D017971:Tin Compounds; C033493:technetium Tc 99m tin colloid; D014001:Tin; D011205:Povidone
keywords: 99mTc-tin Colloid; Lymphoscintigraphy; Polyvinylpyrrolidone (PVP); Sentinel Lymph Node