title: Obesity-associated microRNA-26b regulates the proliferation of human preadipocytes via arrest of the G1/S transition.
abstract: MicroRNAs (miRNAs) are short, 20‑24 nucleotide non‑coding RNAs, which are involved in multiple biological processes, including obesity. Our previous investigation revealed that miRNA (miR)‑26b is differentially expressed in preadipocytes and mature adipocytes in humans. However, its role in the proliferation of human preadipocytes remains to be fully elucidated. In the present study, intracellular lipid accumulation was assessed using oil red O staining and the trigycerlide (TG) content was quantified using a TG assay kit, adipogenesis associated genes and cyclin D2 were analyzed using western blotting, and the effects of miR‑26b on the proliferation of preadipocytes was investigated using Cell Counting Kit‑8 assays and cell cycle analysis. Human preadipocytes overexpressing miR‑26b exhibited increased TG content in the adipocytes. During differentiation, the protein expression levels of adipogenesis‑associated marker genes, including peroxisome proliferator‑activated receptor γ, CCAAT/enhancer‑binding protein α, fatty acid‑binding protein and hormone‑sensitive lipase were upregulated in cells overexpressing miR‑26b, compared with the negative control cells. In addition, growth of human preadipocytes overexpressing miR‑26b occurred a slower rate and more remained in the G1 phase, compared with the negative control cells. In addition, miR‑26b downregulated the protein expression of cyclin D2. These results demonstrated that miR‑26b promoted differentiation and, at least party by targeting cyclin D2, attenuated cell proliferation via arresting the G1/S transition.
authors.affiliation: Department of Laboratory Medicine, The 82nd Hospital of The People's Liberation Army, Huaian, Jiangsu 223001, P.R. China.
authors.forename: Guangfeng
authors.initials: G
authors.lastname: Xu
authors.affiliation: Department of Child Health Care, Affiliated Nanjing Maternity and Child Health Care Hospital, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China.
authors.forename: Chenbo
authors.initials: C
authors.lastname: Ji
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China.
authors.forename: Guixian
authors.initials: G
authors.lastname: Song
authors.affiliation: Institute of Pediatrics, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Shi
authors.affiliation: Department of Child Health Care, Affiliated Nanjing Maternity and Child Health Care Hospital, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China.
authors.forename: Yahui
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Institute of Pediatrics, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China.
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.affiliation: Institute of Pediatrics, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Laboratory Medicine, The 82nd Hospital of The People's Liberation Army, Huaian, Jiangsu 223001, P.R. China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Child Health Care, Affiliated Nanjing Maternity and Child Health Care Hospital, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China.
authors.forename: Xirong
authors.initials: X
authors.lastname: Guo
mesh_terms: D017667:Adipocytes / Q000378:metabolism / Q000473:pathology*; D050156:Adipogenesis; D002460:Cell Line; D049109:Cell Proliferation; D059585:G1 Phase Cell Cycle Checkpoints; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009765:Obesity / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D059807:S Phase Cell Cycle Checkpoints; D014280:Triglycerides / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D035683:MicroRNAs; D014280:Triglycerides; C528163:MIRN26 microRNA, human