title: Stain-less staining for computed histopathology.
abstract: Dyes such as hematoxylin and eosin (H&E) and immunohistochemical stains have been increasingly used to visualize tissue composition in research and clinical practice. We present an alternative approach to obtain the same information using stain-free chemical imaging. Relying on Fourier transform infrared (FT-IR) spectroscopic imaging and computation, stainless computed histopathology can enable a rapid, digital, quantitative and non-perturbing visualization of morphology and multiple molecular epitopes simultaneously in a variety of research and clinical pathology applications.
authors.affiliation: Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
authors.forename: David
authors.initials: D
authors.lastname: Mayerich
authors.affiliation: Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. ; Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801 and Carle Foundation Hospital, Urbana, IL 61801, USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Walsh
authors.affiliation: Department of Pathology and University of Illinois Cancer Center, University of Illinois at Chicago, Chicago, IL 60612, USA.
authors.forename: Andre
authors.initials: A
authors.lastname: Kadjacsy-Balla
authors.affiliation: Carle Cancer Center, Carle Foundation Hospital, Urbana, IL and Department of Surgery, and University of Illinois Cancer Center, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
authors.forename: Partha S
authors.initials: PS
authors.lastname: Ray
authors.affiliation: Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Hewitt
authors.affiliation: Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. ; Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801 and Carle Foundation Hospital, Urbana, IL 61801, USA. ; Departments of Mechanical Science and Engineering, Electrical and Computer Engineering, Chemical and Biomolecular Engineering and Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
authors.forename: Rohit
authors.initials: R
authors.lastname: Bhargava