title: Engineering of yeast pyruvate decarboxylase for enhanced selectivity towards carboligation.
abstract: The aim of the study was to increase production of (R)-PAC by altering carboligation activity of Pdc in Saccharomyces cerevisiae. Pdc1 activity was modified by over-expression as well as changing the rate of decarboxylation and carboligation by site specific mutation in Pdc1. Over-expression of mutant Pdc1 resulted in 50 ± 2.5% increase in levels of (R)-PAC in wild-type and further 30-40% in pdc null background. The combination of mutant Pdc1 in pdc null background was successfully evaluated for production of (R)-PAC at industrial scale. This is the first report of enhancing (R)-PAC product in yeast by recombinant technology with capability of commercial production.
authors.affiliation: Department of Biosciences and Bioengineering, IIT Bombay, Powai 40076, Mumbai, India. Electronic address: Praveen.Agarwal@gennova.co.in.
authors.forename: Praveen Kumar
authors.initials: PK
authors.lastname: Agarwal
authors.affiliation: Department of Biosciences and Bioengineering, IIT Bombay, Powai 40076, Mumbai, India. Electronic address: vanita.uppada@gmail.com.
authors.forename: Vanita
authors.initials: V
authors.lastname: Uppada
authors.affiliation: Embio Limited, Mumbai 400076, India. Electronic address: swami@embio.co.in.
authors.forename: A G
authors.initials: AG
authors.lastname: Swaminathan
authors.affiliation: Department of Chemical engineering, IIT Bombay, Powai 40076, Mumbai, India. Electronic address: noronha@iitb.ac.in.
authors.forename: Santosh B
authors.initials: SB
authors.lastname: Noronha
mesh_terms: D003653:Decarboxylation / Q000235:genetics; D000432:Methanol / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D011767:Pyruvate Decarboxylase / Q000235:genetics* / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D029701:Saccharomyces cerevisiae Proteins; C101504:PDC1 protein, S cerevisiae; D011767:Pyruvate Decarboxylase; D000432:Methanol
keywords: (R)-phenyl acetyl carbinol; Biotransformation; Carboligation; Decarboxylation; Pyruvate decarboxylase