title: Grape powder treatment prevents anxiety-like behavior in a rat model of aging.
abstract: Earlier, we have reported that grape powder (GP) treatment prevented pharmacologic and psychological stress-induced anxiety-like behavior and memory impairment in rats. Protective effects of GP were attributed to its antioxidant effects. In this study, we tested the hypothesis that age-associated behavioral and cognitive deficits such as anxiety and memory impairment will be ameliorated with GP treatment. Using a National Institute of Aging recommended rodent model of aging, we examined a potentially protective role of antioxidant-rich GP in age-associated anxiety-like behavior and memory impairment. Male Fischer 344 rats were randomly assigned into 4 groups: young rats (3 months old) provided with tap water or with 15 g/L GP dissolved in tap water for 3 weeks, aged rats (21 months old) provided with tap water or with GP-treated tap water for 3 weeks (AG-GP). Anxiety-like behavior was significantly greater in aged rats compared with young rats, GP-treated young rats, or aged control rats (P < .05). Also, GP treatment prevented age-induced anxiety-like behavior in AG-GP rats (P < .05). Neither short-term nor long-term age-associated memory deficits improved with GP treatment in AG-GP rats. Furthermore, aged rats showed increased level of physiological stress (corticosterone) and increased oxidative stress in the plasma (8-isoprostane) as well as in selected brain areas (protein carbonylation). Grape powder treatment prevented age-induced increase in corticosterone levels and plasma 8-isoprostane levels in aged rats (P < .05), whereas protein carbonylation was recovered in the amygdala region only (P < .05). Grape powder by regulating oxidative stress ameliorates age-induced anxiety-like behavior in rats, whereas age-associated memory deficits seem unaffected with GP treatment.
authors.affiliation: Department of Pharmacological and Pharmaceutical Sciences, University of Houston, Houston, TX, USA.
authors.forename: Gaurav
authors.initials: G
authors.lastname: Patki
authors.affiliation: Department of Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.
authors.forename: Quaisar
authors.initials: Q
authors.lastname: Ali
authors.affiliation: Department of Pharmacological and Pharmaceutical Sciences, University of Houston, Houston, TX, USA.
authors.forename: Indira
authors.initials: I
authors.lastname: Pokkunuri
authors.affiliation: Department of Pharmacological and Pharmaceutical Sciences, University of Houston, Houston, TX, USA. Electronic address: masghar@uh.edu.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Asghar
authors.affiliation: Department of Pharmacological and Pharmaceutical Sciences, University of Houston, Houston, TX, USA. Electronic address: ssalim@uh.edu.
authors.forename: Samina
authors.initials: S
authors.lastname: Salim
mesh_terms: D000375:Aging; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D001007:Anxiety / Q000517:prevention & control*; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D003345:Corticosterone / Q000097:blood; D015237:Dinoprost / Q000031:analogs & derivatives / Q000097:blood; D005638:Fruit; D008297:Male; D008569:Memory Disorders / Q000209:etiology / Q000517:prevention & control; D023421:Models, Animal; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D011208:Powders; D011916:Rats, Inbred F344; D013312:Stress, Physiological; D013315:Stress, Psychological / Q000517:prevention & control; D027843:Vitis
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D011208:Powders; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost; D003345:Corticosterone
keywords: Aging; Anxiety; Cognition; Grape powder; Psychological stress