title: Detection of a divergent variant of grapevine virus F by next-generation sequencing.
abstract: The complete genome sequence of a South African isolate of grapevine virus F (GVF) is presented. It was first detected by metagenomic next-generation sequencing of field samples and validated through direct Sanger sequencing. The genome sequence of GVF isolate V5 consists of 7539 nucleotides and contains a poly(A) tail. It has a typical vitivirus genome arrangement that comprises five open reading frames (ORFs), which share only 88.96 % nucleotide sequence identity with the existing complete GVF genome sequence (JX105428).
authors.affiliation: Department of Genetics, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Molenaar
authors.forename: Johan T
authors.initials: JT
authors.lastname: Burger
authors.forename: Hans J
authors.initials: HJ
authors.lastname: Maree
mesh_terms: D052677:Flexiviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D014644:Genetic Variation; D016679:Genome, Viral; D059014:High-Throughput Nucleotide Sequencing; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D017423:Sequence Analysis, RNA; D013019:South Africa; D027843:Vitis / Q000821:virology*