title: MYC protein expression is associated with poor prognosis in primary diffuse large B-cell lymphoma of the central nervous system.
abstract: MYC and BCL2 gene translocations and protein expression have recently demonstrated to be of prognostic significance in systemic diffuse large B-cell lymphoma (DLBCL). However, their role in primary central nervous system DLBCL (CNS-DLBCL) prognosis has been scarcely analyzed. We studied the immunophenotype, the status of the MYC, BCL2, and BCL6 genes and the clinical features of a series of 42 CNS-DLBCL and evaluated their prognostic significance. We found high MYC protein expression in 43% of cases, and this was associated with lower overall survival (OS). Cases with concurrent expression of MYC and BCL2 showed a lower OS, although the difference did not reach statistical significance. Translocations involving the MYC or BCL2 genes were not detected. The BCL6 gene was frequently translocated, but was unrelated to survival. We conclude that MYC protein expression detected by immunohistochemistry identifies a CNS-DLBCL subset with worse prognosis and may contribute to a more accurate risk stratification of CNS-DLBCL patients.
authors.affiliation: Department of Pathology, Hospital Germans Trias i Pujol, Universitat Autonoma de Barcelona, Badalona, Spain.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Tapia
authors.affiliation: Department of Hematology, ICO- Hospital Germans Trias i Pujol, Josep Carreras Leukaemia Research Institute, Universitat Autònoma de Barcelona, Badalona, Spain.
authors.forename: Maria-Joao
authors.initials: MJ
authors.lastname: Baptista
authors.affiliation: Department of Pathology, Hospital Germans Trias i Pujol, Universitat Autonoma de Barcelona, Badalona, Spain.
authors.forename: Ana-Maria
authors.initials: AM
authors.lastname: Muñoz-Marmol
authors.affiliation: Department of Pathology, Hospital Universitario Cruces, Barakaldo, Spain.
authors.forename: Ayman
authors.initials: A
authors.lastname: Gaafar
authors.affiliation: Department of Hematology, Hospital Universitario Cruces, Barakaldo, Spain.
authors.forename: Maria
authors.initials: M
authors.lastname: Puente-Pomposo
authors.affiliation: Department of Hematology, ICO- Hospital Germans Trias i Pujol, Josep Carreras Leukaemia Research Institute, Universitat Autònoma de Barcelona, Badalona, Spain.
authors.forename: Olga
authors.initials: O
authors.lastname: Garcia
authors.affiliation: Department of Pathology, Hospital Germans Trias i Pujol, Universitat Autonoma de Barcelona, Badalona, Spain.
authors.forename: Ruth
authors.initials: R
authors.lastname: Marginet-Flinch
authors.affiliation: Department of Pathology, Hospital Germans Trias i Pujol, Universitat Autonoma de Barcelona, Badalona, Spain.
authors.forename: Carolina
authors.initials: C
authors.lastname: Sanz
authors.affiliation: Department of Hematology, ICO- Hospital Germans Trias i Pujol, Josep Carreras Leukaemia Research Institute, Universitat Autònoma de Barcelona, Badalona, Spain.
authors.forename: Jose-Tomas
authors.initials: JT
authors.lastname: Navarro
authors.affiliation: Department of Hematology, ICO- Hospital Germans Trias i Pujol, Josep Carreras Leukaemia Research Institute, Universitat Autònoma de Barcelona, Badalona, Spain.
authors.forename: Juan-Manuel
authors.initials: JM
authors.lastname: Sancho
authors.affiliation: Department of Hematology, ICO- Hospital Germans Trias i Pujol, Josep Carreras Leukaemia Research Institute, Universitat Autònoma de Barcelona, Badalona, Spain.
authors.forename: Josep-Maria
authors.initials: JM
authors.lastname: Ribera
authors.affiliation: Department of Pathology, Hospital Germans Trias i Pujol, Universitat Autonoma de Barcelona, Badalona, Spain.
authors.forename: Aurelio
authors.initials: A
authors.lastname: Ariza
authors.affiliation: Department of Pathology, Hospital Germans Trias i Pujol, Universitat Autonoma de Barcelona, Badalona, Spain.
authors.forename: Jose-Luis
authors.initials: JL
authors.lastname: Mate
mesh_terms: D000328:Adult; D002490:Central Nervous System / Q000166:cytology / Q000473:pathology*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D016403:Lymphoma, Large B-Cell, Diffuse / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D008297:Male; D011379:Prognosis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D051560:Proto-Oncogene Proteins c-bcl-6; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism*; D012189:Retrospective Studies
chemical_list: C000595707:BCL2 protein, human; C084103:BCL6 protein, human; D004268:DNA-Binding Proteins; C489427:MYC protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D051560:Proto-Oncogene Proteins c-bcl-6; D016271:Proto-Oncogene Proteins c-myc
keywords: BCL2; MYC; immunohistochemistry; primary CNS lymphoma; prognosis