title: OsWS1 involved in cuticular wax biosynthesis is regulated by osa-miR1848.
abstract: Cuticular wax forms a hydrophobic layer covering aerial plant organs and acting as a protective barrier against biotic and abiotic stresses. Compared with well-known wax biosynthetic pathway, molecular regulation of wax biosynthesis is less known. Here, we show that rice OsWS1, a member of the membrane-bound O-acyl transferase gene family, involved in wax biosynthesis and was regulated by an osa-miR1848. OsWS1-tagged green fluorescent protein localized to the endoplasmic reticulum (ER). Compared with wild-type rice, OsWS1 overexpression plants displayed a 3% increase in total wax, especially a 35% increase in very long-chain fatty acids, denser wax papillae around the stoma, more cuticular wax crystals formed on leaf and stem surfaces, pollen coats were thicker and more seedlings survived after water-deficit treatment. In contrast, OsWS1-RNAi and osa-miR1848 overexpression plants exhibited opposing changes. Gene expression analysis showed that overexpression of osa-miR1848 down-regulated OsWS1 transcripts; furthermore, expression profiles of OsWS1 and osa-miR1848 were inversely correlated in the leaf, panicle and stem, and upon water-deficit treatment. These results suggest that OsWS1 is regulated by osa-miR1848 and participates in cuticular wax formation.
authors.affiliation: Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China.
authors.forename: Kuaifei
authors.initials: K
authors.lastname: Xia
authors.affiliation: Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China.|Department of Biology, University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Xiaojin
authors.initials: X
authors.lastname: Ou
authors.affiliation: Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China.|Department of Biology, University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Chunzhi
authors.initials: C
authors.lastname: Gao
authors.affiliation: Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China.|Department of Biology, University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Huadan
authors.initials: H
authors.lastname: Tang
authors.affiliation: Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China.
authors.forename: Yongxia
authors.initials: Y
authors.lastname: Jia
authors.affiliation: Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China.
authors.forename: Rufang
authors.initials: R
authors.lastname: Deng
authors.affiliation: Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China.
authors.forename: Xinlan
authors.initials: X
authors.lastname: Xu
authors.affiliation: Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China.
authors.forename: Mingyong
authors.initials: M
authors.lastname: Zhang
mesh_terms: D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism; D002462:Cell Membrane / Q000201:enzymology; D004721:Endoplasmic Reticulum / Q000201:enzymology; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D035683:MicroRNAs / Q000235:genetics*; D012275:Oryza / Q000166:cytology / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D019441:Plant Epidermis / Q000166:cytology / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D018515:Plant Leaves / Q000166:cytology / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018547:Plant Stems / Q000166:cytology / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D036226:Seedlings / Q000166:cytology / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D014885:Waxes / Q000378:metabolism*
chemical_list: D035683:MicroRNAs; D010940:Plant Proteins; D014885:Waxes; D000217:Acyltransferases
keywords: cuticle; drought; microRNA; rice; wax