title: Modified expression of peripheral blood lymphocyte muscarinic cholinergic receptors in asthmatic children.
abstract: Lymphocytes possess an independent cholinergic system. We assessed the expression of muscarinic cholinergic receptors in lymphocytes from 49 asthmatic children and 10 age matched controls using Western blot. We demonstrated that CD4+ and CD8+ T cells expressed M2 and M4 muscarinic receptors which density were significantly increased in asthmatic children in comparison with controls. M2 and M4 receptor increase was strictly related with IgE and fraction of exhaled nitric oxide (FeNO) measurements and with impairment in objective measurements of airway obstruction. Increased lymphocyte muscarinic cholinergic receptor expression may concur with lung cholinergic dysfunction and with inflammatory molecular framework in asthma.
authors.affiliation: Dipartimento di Scienze Mediche e Molecolari, Università la Sapienza, Italy; Centro Ricerche Ospedale San Pietro, Roma, Italy.
authors.forename: Emanuela
authors.initials: E
authors.lastname: Cherubini
authors.affiliation: Dipartimento di Scienze Mediche e Molecolari, Università la Sapienza, Italy; Centro Ricerche Ospedale San Pietro, Roma, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Tabbì
authors.affiliation: Dipartimento di Scienze Mediche e Molecolari, Università la Sapienza, Italy; Centro Ricerche Ospedale San Pietro, Roma, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Scozzi
authors.affiliation: Dipartimento di Scienze Mediche e Molecolari, Università la Sapienza, Italy; Centro Ricerche Ospedale San Pietro, Roma, Italy.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Mariotta
authors.affiliation: Centro Ricerche Ospedale San Pietro, Roma, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Galli
authors.affiliation: Centro Ricerche Ospedale San Pietro, Roma, Italy.
authors.forename: Rossella
authors.initials: R
authors.lastname: Carello
authors.affiliation: Centro Ricerche Ospedale San Pietro, Roma, Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: Avitabile
authors.affiliation: Sezione di Anatomia Umana, Dipartimento di Scienze Farmacologiche e Medicina Sperimentale, Università di Camerino, Italy.
authors.forename: Seyed Koshrow
authors.initials: SK
authors.lastname: Tayebati
authors.affiliation: Sezione di Anatomia Umana, Dipartimento di Scienze Farmacologiche e Medicina Sperimentale, Università di Camerino, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Amenta
authors.affiliation: Dipartimento di chirurgia "P.Valdoni," Sapienza University, 00161 Rome, Italy; IRCCS Istituto Nazionale Tumori, Fondazione G. Pascale, 80131 Naples, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: De Vitis
authors.affiliation: Dipartimento di Scienze Mediche e Molecolari, Università la Sapienza, Italy.
authors.forename: Rita
authors.initials: R
authors.lastname: Mancini
authors.affiliation: Dipartimento di Scienze Mediche e Molecolari, Università la Sapienza, Italy; Centro Ricerche Ospedale San Pietro, Roma, Italy. Electronic address: alberto.ricci@uniroma1.it.
authors.forename: Alberto
authors.initials: A
authors.lastname: Ricci
mesh_terms: D000280:Administration, Inhalation; D000328:Adult; D000367:Age Factors; D001249:Asthma / Q000097:blood / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology*; D001993:Bronchodilator Agents / Q000008:administration & dosage; D015704:CD4 Antigens / Q000378:metabolism; D016827:CD8 Antigens / Q000378:metabolism; D016022:Case-Control Studies; D002648:Child; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D016014:Linear Models; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009569:Nitric Oxide / Q000008:administration & dosage; D011976:Receptors, Muscarinic / Q000235:genetics / Q000378:metabolism*; D012720:Severity of Illness Index; D018709:Statistics, Nonparametric
chemical_list: D001993:Bronchodilator Agents; D015704:CD4 Antigens; D016827:CD8 Antigens; D011976:Receptors, Muscarinic; D009569:Nitric Oxide
keywords: Asthma; Human; Muscarinic receptors; Western blot