title: Increased Vancomycin Susceptibility in Mycobacteria: a New Approach To Identify Synergistic Activity against Multidrug-Resistant Mycobacteria.
abstract: Mycobacterium tuberculosis is wrapped in complex waxes, impermeable to most antibiotics. Comparing Mycobacterium bovis BCG and M. tuberculosis mutants that lack phthiocerol dimycocerosates (PDIM) and/or phenolic glycolipids with wild-type strains, we observed that glycopeptides strongly inhibited PDIM-deprived mycobacteria. Vancomycin together with a drug targeting lipid synthesis inhibited multidrug-resistant (MDR) and extensively drug-resistant (XDR) clinical isolates. Our study puts glycopeptides in the pipeline of potential antituberculosis (TB) agents and might provide a new antimycobacterial drug-screening strategy.
authors.affiliation: Scientific Institute of Public Health (WIV-ISP), Operational Direction of Communicable and Infectious Diseases, Brussels, Belgium.
authors.forename: Karine
authors.initials: K
authors.lastname: Soetaert
authors.affiliation: Université Libre de Bruxelles (ULB), Unit of Pharmaceutical Microbiology and Hygiene, Brussels, Belgium.
authors.forename: Céline
authors.initials: C
authors.lastname: Rens
authors.affiliation: Scientific Institute of Public Health (WIV-ISP), Operational Direction of Communicable and Infectious Diseases, Brussels, Belgium.
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Wang
authors.affiliation: Université Libre de Bruxelles (ULB), Unit of Pharmaceutical Microbiology and Hygiene, Brussels, Belgium.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: De Bruyn
authors.affiliation: Tuberculosis and Infection Biology (TBIB), Institute of Pharmacology and Structural Biology of CNRS and the University Paul Sabatier (UMR 5089), Toulouse, France.
authors.forename: Marie-Antoinette
authors.initials: MA
authors.lastname: Lanéelle
authors.affiliation: Tuberculosis and Infection Biology (TBIB), Institute of Pharmacology and Structural Biology of CNRS and the University Paul Sabatier (UMR 5089), Toulouse, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Laval
authors.affiliation: Tuberculosis and Infection Biology (TBIB), Institute of Pharmacology and Structural Biology of CNRS and the University Paul Sabatier (UMR 5089), Toulouse, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Lemassu
authors.affiliation: Tuberculosis and Infection Biology (TBIB), Institute of Pharmacology and Structural Biology of CNRS and the University Paul Sabatier (UMR 5089), Toulouse, France.
authors.forename: Mamadou
authors.initials: M
authors.lastname: Daffé
authors.affiliation: Novartis Institute for Tropical Diseases (NITD), Singapore, Singapore.
authors.forename: Pablo
authors.initials: P
authors.lastname: Bifani
authors.affiliation: Université Libre de Bruxelles (ULB), Unit of Pharmaceutical Microbiology and Hygiene, Brussels, Belgium.
authors.forename: Véronique
authors.initials: V
authors.lastname: Fontaine
authors.affiliation: Université Libre de Bruxelles (ULB), Unit of Pharmaceutical Microbiology and Hygiene, Brussels, Belgium philefev@ulb.ac.be.
authors.forename: Philippe
authors.initials: P
authors.lastname: Lefèvre
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology*; D002473:Cell Wall / Q000737:chemistry / Q000187:drug effects; D024901:Drug Resistance, Multiple, Bacterial; D006020:Glycopeptides / Q000494:pharmacology*; D006801:Humans; D008055:Lipids / Q000096:biosynthesis; D008826:Microbial Sensitivity Tests; D009163:Mycobacterium bovis / Q000187:drug effects*; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D014397:Tuberculosis, Pulmonary / Q000188:drug therapy; D014640:Vancomycin / Q000494:pharmacology*
chemical_list: D000995:Antitubercular Agents; D006020:Glycopeptides; D008055:Lipids; D014640:Vancomycin