title: Antioxidant action of SMe1EC2, the low-basicity derivative of the pyridoindole stobadine, in cell free chemical models and at cellular level.
abstract: The aim of the study was to evaluate the antioxidant action of SMe1EC2, the structural analogue of the hexahydropyridoindole antioxidant stobadine. The antiradical activity of SMe1EC2 was found to be higher when compared to stobadine, as determined both in cell-free model systems of AAPH-induced oxidation of dihydrorhodamine 123 and 2',7'-dichloro-dihydrofluorescein diacetate, and in the cellular system of stimulated macrophages RAW264.7. Analysis of proliferation of HUVEC and HUVEC-ST cells revealed absence of cytotoxic effect of SMe1EC2 at concentrations below 100 µM. The antioxidant activity of SMe1EC2, superior to the parent drug stobadine, is accounted for by both the higher intrinsic free radical scavenging action and by the better bioavailability of the low-basicity SMe1EC2 relative to the high-basicity stobadine.
authors.affiliation: Department of Molecular Biophysics, University of Lodz, Lodz, Poland.
authors.forename: Aneta
authors.initials: A
authors.lastname: Balcerczyk
authors.affiliation: Department of Molecular Biophysics, University of Lodz, Lodz, Poland ; Department of Biochemistry and Cell Biology, University of Rzeszow, Rzeszow, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Bartosz
authors.affiliation: Department of Molecular Biophysics, University of Lodz, Lodz, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Drzewinska
authors.affiliation: Department of Molecular Biophysics, University of Lodz, Lodz, Poland.
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Piotrowski
authors.affiliation: Department of Molecular Biophysics, University of Lodz, Lodz, Poland ; Laboratory of Transcriptional Regulation, Center of Medical Biology, PAS, Lodz, Poland.
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Pulaski
authors.affiliation: Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Bratislava, Slovakia.
authors.forename: Milan
authors.initials: M
authors.lastname: Stefek
keywords: SMe1EC2; antioxidant; hexahydropyridoindole; oxidative stress; stobadine