title: Association of the expression levels in the longissimus muscle and a SNP in the CDC10 gene with marbling in Japanese Black beef cattle.
abstract: The septin 7 (CDC10) gene, involved in cellular proliferation, has been previously shown to be expressed at different levels in the longissimus muscle (LM) between low-marbled and high-marbled steer groups by differential-display PCR. It is located within the genomic region of a quantitative trait locus for marbling, and thus was considered as a positional functional candidate gene for marbling. In this study, we showed that the CDC10 expression levels in the LM were positively correlated with marbling in Japanese Black (JB) steers (P<0.0001). Further, an association analysis indicated that a SNP in the promoter region of the CDC10 gene was associated with marbling using 99 JB sires (P=0.03) and 542 JB paternal half-sib progeny steers from a sire homozygous for G allele at the SNP (P<0.0001). These findings suggest possible effects of the expression levels in the LM and the SNP of the CDC10 gene on marbling in JB cattle.
authors.affiliation: The Key Laboratory for Mammalian Reproductive Biology and Biotechnology, Ministry of Education, Inner Mongolia University, Hohhot, China; Department of Agrobiology, Faculty of Agriculture, Niigata University, Nishi-ku, Niigata, Japan.
authors.forename: B
authors.initials: B
authors.lastname: Tong
authors.affiliation: The Key Laboratory for Mammalian Reproductive Biology and Biotechnology, Ministry of Education, Inner Mongolia University, Hohhot, China.
authors.forename: G Q
authors.initials: GQ
authors.lastname: Gao
authors.affiliation: Department of Nutritional Sciences for Well-being, Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, Kashiwara, Osaka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Muramatsu
authors.affiliation: Central Pharmaceutical Research Institute, Japan Tobacco, Inc., Takatsuki, Osaka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ohta
authors.affiliation: Department of Life Science, Division of Natural Sciences, International Christian University, Mitaka, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kose
authors.affiliation: The Key Laboratory for Mammalian Reproductive Biology and Biotechnology, Ministry of Education, Inner Mongolia University, Hohhot, China.
authors.forename: G P
authors.initials: GP
authors.lastname: Li
authors.affiliation: Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, East Java, Indonesia.
authors.forename: F
authors.initials: F
authors.lastname: Fatchiyah
authors.affiliation: Department of Agrobiology, Faculty of Agriculture, Niigata University, Nishi-ku, Niigata, Japan. Electronic address: tyamada@agr.niigata-u.ac.jp.
authors.forename: T
authors.initials: T
authors.lastname: Yamada
mesh_terms: D000483:Alleles; D000818:Animals; D002417:Cattle / Q000235:genetics*; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D060005:Genotyping Techniques; D006720:Homozygote; D018482:Muscle, Skeletal / Q000378:metabolism*; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D040641:Quantitative Trait Loci; D058112:Septins / Q000235:genetics* / Q000378:metabolism
chemical_list: D018797:Cell Cycle Proteins; D058112:Septins
keywords: Association; Beef cattle; CDC10; Expression; Marbling; Single nucleotide polymorphism