title: Adenosinergic regulation of binge-like ethanol drinking and associated locomotor effects in male C57BL/6J mice.
abstract: We recently observed that the addition of caffeine (a nonselective adenosine receptor antagonist) to a 20% ethanol solution significantly altered the intoxication profile of male C57BL/6J (B6) mice induced by voluntary binge-like consumption in the 'Drinking-in-the-Dark' (DID) paradigm. In the current study, the roles of A1 and A2A adenosine receptor subtypes, specifically, in binge-like ethanol consumption and associated locomotor effects were explored. Adult male B6 mice (PND 60-70) were allowed to consume 20% ethanol (v/v) or 2% sucrose (w/v) for 6days via DID. On day 7, mice received a systemic administration (i.p.) of the A1 antagonist DPCPX (1, 3, 6mg/kg), the A2A antagonist MSX-3 (1, 2, 4mg/kg), or vehicle immediately prior to fluid access in DID. Antagonism of the A1 receptor via DPCPX was found to dose-dependently decrease binge-like ethanol intake and associated blood ethanol concentrations (p's<0.05), although no effect was observed on sucrose intake. Antagonism of A2A had no effect on ethanol or sucrose consumption, however, MSX-3 elicited robust locomotor stimulation in mice consuming either solution (p's<0.05). Together, these findings suggest unique roles for the A1 and A2A adenosine receptor subtypes in binge-like ethanol intake and its associated locomotor effects.
authors.affiliation: Indiana Alcohol Research Center, Department of Psychology, Indiana University-Purdue University Indianapolis, IN, United States. Electronic address: bmfritz@iupui.edu.
authors.forename: Brandon M
authors.initials: BM
authors.lastname: Fritz
authors.affiliation: Indiana Alcohol Research Center, Department of Psychology, Indiana University-Purdue University Indianapolis, IN, United States.
authors.forename: Stephen L
authors.initials: SL
authors.lastname: Boehm
mesh_terms: D000241:Adenosine / Q000502:physiology*; D058916:Adenosine A1 Receptor Antagonists / Q000494:pharmacology; D058917:Adenosine A2 Receptor Antagonists / Q000494:pharmacology; D000428:Alcohol Drinking / Q000188:drug therapy / Q000523:psychology; D000818:Animals; D063425:Binge Drinking / Q000523:psychology*; D002110:Caffeine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009043:Motor Activity / Q000187:drug effects*; D018047:Receptors, Purinergic P1 / Q000502:physiology*; D013395:Sucrose; D014970:Xanthines / Q000494:pharmacology
chemical_list: D058916:Adenosine A1 Receptor Antagonists; D058917:Adenosine A2 Receptor Antagonists; C113602:MSX 3 compound; D018047:Receptors, Purinergic P1; D014970:Xanthines; D002110:Caffeine; D013395:Sucrose; C051360:1,3-dipropyl-8-cyclopentylxanthine; D000241:Adenosine
keywords: Adenosine; Alcohol; Binge drinking; Drinking-in-the-dark; Ethanol; Mouse