title: [β-arrestin1 promotes chronic myeloid leukemia cell proliferation by activating JNK signaling pathway].
abstract: OBJECTIVE
  To investigate the signaling pathways involved in β-arrestin1-induced proliferation of K562 cells.
  
  
  METHODS
  We established stable cell lines K562-siβ1 and K562-β1 by lentivirus-mediated β-arrestin1 knock-down or overexpression in K562 cells, with cells transfected with non-specific siRNA as the control (K562-Ctrl). The proliferation of these cells were evaluated by cell counting and CCK-8 assays. Western blotting was used to detect the expression of JNK and p-JNK in the cells, and co-immunoprecipitation (Co-IP) assay was employed to investigate the interaction between β-arrestin1 and Src.
  
  
  RESULTS
  K562-β1 cells showed significantly greater but K562-siβ1 cells had significantly lower proliferation ability and cell survival rate than K562-Ctrl cells. Western blotting showed that β-arrestin1 specifically enhanced the expression of p-JNK, and the JNK inhibitor SP600125 obviously suppressed p-JNK and cell proliferation of K562 cells. Co-IP assay revealed the binding of β-arrestin1 to Src.
  
  
  CONCLUSIONS
  In K562 cells, β-arrestin1 activates JNK signaling pathway by binding to Src to promote the cell proliferation.
authors.affiliation: Center for Clinical Molecular Medicine, Children's Hospital of Chongqing Medical University//Ministry of Education Key Laboratory of Child Development and Disorders//Key Laboratory of Pediatrics in Chongqing//Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing 400014, China.E-mail: stella8848@163.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.forename: Kang
authors.initials: K
authors.lastname: Li
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhenglan
authors.initials: Z
authors.lastname: Tan
authors.forename: Lin
authors.initials: L
authors.lastname: Zou
mesh_terms: D019390:Arrestins / Q000378:metabolism*; D049109:Cell Proliferation; D002470:Cell Survival; D006801:Humans; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000378:metabolism*; D020935:MAP Kinase Signaling System; D034741:RNA, Small Interfering; D000071557:beta-Arrestins
chemical_list: D019390:Arrestins; D034741:RNA, Small Interfering; D000071557:beta-Arrestins