title: Bleed-to-read disposable microsystems for the genetic and serological analysis of celiac disease markers with amperometric detection.
abstract: Celiac disease is an auto-immune disorder induced by ingestion of gluten in genetically predisposed individuals. Its diagnostics is more accurate using a combination of immunologic and genetic tests to detect of high levels of certain auto-antibodies and the presence human leukocyte antigen HLA-DQ2 or HLA-DQ8 genetic markers. In this work, we report the design and testing of automated microsystems combining sample treatment, storage, fluidic transport, and detection in a single platform able to carry out genetic or serologic analysis for detection of celiac disease markers. These microsystems share a common footprint and many fluidic features and are thus able to perform a complete assay. The microsystem for the genetic assay extracts and amplifies the DNA prior to detection, while the serology microsystem contains a filter and chamber for the generation and subsequent dilution of plasma. The performance of both platforms is demonstrated and compared with reference methods with an excellent correlation, which makes the developed platform amenable for clinical studies.
authors.affiliation: Nanobiotechnology and Bioanalysis Group, Departament d'Enginyeria Química, Universitat Rovira i Virgili, Tarragona, Spain.
authors.forename: Mayreli
authors.initials: M
authors.lastname: Ortiz
authors.affiliation: Nanobiotechnology and Bioanalysis Group, Departament d'Enginyeria Química, Universitat Rovira i Virgili, Tarragona, Spain.
authors.forename: Hamdi
authors.initials: H
authors.lastname: Joda
authors.affiliation: Fraunhofer ICT-IMM, Mainz, Germany.
authors.forename: Julian
authors.initials: J
authors.lastname: Höth
authors.affiliation: Nanobiotechnology and Bioanalysis Group, Departament d'Enginyeria Química, Universitat Rovira i Virgili, Tarragona, Spain.
authors.forename: Valerio
authors.initials: V
authors.lastname: Beni
authors.affiliation: Nanobiotechnology and Bioanalysis Group, Departament d'Enginyeria Química, Universitat Rovira i Virgili, Tarragona, Spain.
authors.forename: Ioanis
authors.initials: I
authors.lastname: Katakis
authors.affiliation: Microfluidic ChipShop GmbH, Jena, Germany.
authors.forename: Richard
authors.initials: R
authors.lastname: Klemm
authors.affiliation: TATAA Biocenter AB, Göteborg, Sweden.
authors.forename: Kristina
authors.initials: K
authors.lastname: Lind
authors.affiliation: Nanobiotechnology and Bioanalysis Group, Departament d'Enginyeria Química, Universitat Rovira i Virgili, Tarragona, Spain.|Institució Catalana de Recerca i Estudis Avançats, Passeig Lluis Companys, Barcelona, Spain.
authors.forename: Ciara K
authors.initials: CK
authors.lastname: O'Sullivan
authors.affiliation: Nanobiotechnology and Bioanalysis Group, Departament d'Enginyeria Química, Universitat Rovira i Virgili, Tarragona, Spain.
authors.forename: Alex
authors.initials: A
authors.lastname: Fragoso
mesh_terms: D001323:Autoantibodies / Q000097:blood; D015415:Biomarkers / Q000097:blood*; D002446:Celiac Disease / Q000097:blood* / Q000235:genetics; D055664:Electrochemical Techniques / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D005821:Genetic Techniques / Q000295:instrumentation*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D056656:Lab-On-A-Chip Devices
chemical_list: D001323:Autoantibodies; D015415:Biomarkers
keywords: Automated bioassay; Celiac disease; Genosensor; Immunosensor; Integrated microsystem; Lab-on-a-chip