title: Targeted genome editing in primate embryos.
abstract: Mosaic mutations and off-target effects caused by CRISPR/Cas9 have led to concerns about the efficiency and specificity of this new technique in non-human primates and other large animals. Here we discuss recent findings from primate embryos, with a focus on the technical issues CRISPR/Cas9 faces before producing non-human primate models of human diseases.
authors.affiliation: Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Guo
authors.affiliation: 1] Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China [2] Department of Human Genetics, Emory University School of Medicine, Atlanta, GA 30322, USA.
authors.forename: Xiao-Jiang
authors.initials: XJ
authors.lastname: Li
mesh_terms: D000818:Animals; D001483:Base Sequence / Q000235:genetics; D064113:CRISPR-Cas Systems / Q000235:genetics*; D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics*; D016678:Genome / Q000235:genetics*; D006801:Humans; D009154:Mutation / Q000235:genetics; D011323:Primates / Q000196:embryology*