title: Detection of Chlamydia trachomatis, Ureaplasma urealyticum and Mycoplasma hominis in infertile Bulgarian men with multiplex real-time polymerase chain reaction.
abstract: The effect of Chlamydia trachomatis, Ureaplasma urealyticum and Mycoplasma hominis over the sperm quality is still unclear. The aim of this study was to determine their prevalence in infertile Bulgarian men. A total of 281 men were examined by applying mRT-PCR. The registered prevalence was as follows: C. trachomatis - 13.9%, U. urealyticum - 19.2%, M. hominis - 9.9%. Co-infection was established in eight swabs. This first in Bulgaria to study for detection of chlamydia and mycoplasmas in infertile men by mRT-PCR demonstrates higher prevalence of the tested microorganisms in the infertile group toward the control one.
authors.affiliation: Department of Medical Microbiology, Medical University-Sofia, Sofia, Bulgaria.
authors.forename: Vessela
authors.initials: V
authors.lastname: Ouzounova-Raykova
authors.affiliation: Department of Andrology, University Hospital 'Tsaritsa Yoanna', Medical University-Sofia, Sofia, Bulgaria.
authors.forename: Simeon
authors.initials: S
authors.lastname: Rangelov
authors.affiliation: Department of Andrology, University Hospital 'Tsaritsa Yoanna', Medical University-Sofia, Sofia, Bulgaria.
authors.forename: Iordanka
authors.initials: I
authors.lastname: Ouzounova
authors.affiliation: Department of Medical Microbiology, Medical University-Sofia, Sofia, Bulgaria.
authors.forename: Ivan
authors.initials: I
authors.lastname: Mitov
mesh_terms: D002031:Bulgaria; D016022:Case-Control Studies; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D060085:Coinfection; D004269:DNA, Bacterial / Q000302:isolation & purification; D006801:Humans; D007248:Infertility, Male / Q000382:microbiology*; D008297:Male; D019535:Mycoplasma hominis / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D016869:Ureaplasma Infections / Q000175:diagnosis*; D016990:Ureaplasma urealyticum / Q000302:isolation & purification*; D014521:Urethra / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial
keywords: Chlamydia; Mycoplasmas; infertile men; mRT-PCR; prevalence