title: The Hill analysis and co-ion-driven transporter kinetics.
abstract: Interaction of multiple ligands with a protein or protein complex is a widespread phenomenon that allows for cooperativity. Here, we review the use of the Hill equation, which is commonly used to analyze binding or kinetic data, to analyze the kinetics of ion-coupled transporters and show how the mechanism of transport affects the Hill coefficient. Importantly, the Hill analysis of ion-coupled transporters can provide the exact number of transported co-ions, regardless of the extent of the cooperativity in ion binding.
authors.affiliation: Department of Molecular Microbiology and Department of Membrane Enzymology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9747 AG Groningen, Netherlands d.j.slotboom@rug.nl j.s.lolkema@rug.nl.
authors.forename: Juke S
authors.initials: JS
authors.lastname: Lolkema
authors.affiliation: Department of Molecular Microbiology and Department of Membrane Enzymology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9747 AG Groningen, Netherlands d.j.slotboom@rug.nl j.s.lolkema@rug.nl.
authors.forename: Dirk-Jan
authors.initials: DJ
authors.lastname: Slotboom
mesh_terms: D000818:Animals; D001665:Binding Sites; D002462:Cell Membrane / Q000378:metabolism*; D006801:Humans; D015640:Ion Channel Gating; D017136:Ion Transport; D007700:Kinetics; D008024:Ligands; D008564:Membrane Potentials; D008954:Models, Biological; D011485:Protein Binding; D027981:Symporters / Q000378:metabolism*
chemical_list: D008024:Ligands; D027981:Symporters