title: [Isolation and characterization of Escherichia coli O157 in bovine meat products and cattle in the province of Tucuman].
abstract: Escherichia coli O157 is an emergent pathogen associated with diarrhea, hemorrhagic colitis and hemolytic uremic syndrome. Meat products constitute an important transmission source of this microorganism. The aims of this study were to characterize E. coli O157 isolated from cattle and meat products collected from abattoirs and retail stores, to establish the clonal relatedness among regional isolates and to compare them with those in the national database. Between 2004 and 2013, 169 minced meat, 35 sausage and 216 carcass samples were analyzed. Thirteen E. coli O157 isolates were identified; 6 of which were O157:H7 and characterized as stx2c(vh-a)/eae/ehxA (n = 5) and stx2/eae/ehxA (n = 1). The 7 remaining isolates were non-toxigenic E. coli strains, and serotyped as O157:NT (n = 4), O157:NM (n = 1), O157:ND (n = 1) and O157:H16 (n = 1). The strains yielded different XbaI-PFGE patterns. Compared to the E. coli O157 isolates in the National Database, none of these patterns have been previously detected in strains of different origin in Argentina.
authors.affiliation: Cátedra de Bacteriología, Instituto de Microbiología «Luis C. Verna», Facultad de Bioquímica, Universidad Nacional de Tucumán, San Miguel de Tucumán, Tucumán, Argentina. Electronic address: magejure@gmail.com.
authors.forename: María A
authors.initials: MA
authors.lastname: Jure
authors.affiliation: Dirección de Bromatología, San Miguel de Tucumán, Tucumán, Argentina; Cátedra de Bromatología, Facultad de Bioquímica; Universidad Nacional de Tucumán, San Miguel de Tucumán, Tucumán, Argentina.
authors.forename: Marina S
authors.initials: MS
authors.lastname: Condorí
authors.affiliation: Cátedra de Bacteriología, Instituto de Microbiología «Luis C. Verna», Facultad de Bioquímica, Universidad Nacional de Tucumán, San Miguel de Tucumán, Tucumán, Argentina.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Pérez Terrazzino
authors.affiliation: Cátedra de Bacteriología, Instituto de Microbiología «Luis C. Verna», Facultad de Bioquímica, Universidad Nacional de Tucumán, San Miguel de Tucumán, Tucumán, Argentina.
authors.forename: Mariana G
authors.initials: MG
authors.lastname: Catalán
authors.affiliation: Dirección de Ganadería de la Provincia de Tucumán, San Miguel de Tucumán, Tucumán, Argentina.
authors.forename: Alejandro
authors.initials: A
authors.lastname: López Campo
authors.affiliation: Servicio Fisiopatogenia, Departamento Bacteriología; INEI-ANLIS «Dr. Carlos G. Malbrán», Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Gisella
authors.initials: G
authors.lastname: Zolezzi
authors.affiliation: Servicio Fisiopatogenia, Departamento Bacteriología; INEI-ANLIS «Dr. Carlos G. Malbrán», Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Isabel
authors.initials: I
authors.lastname: Chinen
authors.affiliation: Servicio Fisiopatogenia, Departamento Bacteriología; INEI-ANLIS «Dr. Carlos G. Malbrán», Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Marta
authors.initials: M
authors.lastname: Rivas
authors.affiliation: Cátedra de Bacteriología, Instituto de Microbiología «Luis C. Verna», Facultad de Bioquímica, Universidad Nacional de Tucumán, San Miguel de Tucumán, Tucumán, Argentina.
authors.forename: Marta
authors.initials: M
authors.lastname: Castillo
mesh_terms: D000818:Animals; D001118:Argentina; D015373:Bacterial Typing Techniques; D002417:Cattle / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D004197:Disease Reservoirs; D016521:Electrophoresis, Gel, Pulsed-Field; D004927:Escherichia coli Infections / Q000635:transmission; D019453:Escherichia coli O157 / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D005516:Food Microbiology; D005798:Genes, Bacterial; D008460:Meat / Q000382:microbiology*; D008461:Meat Products / Q000382:microbiology; D012150:Polymorphism, Restriction Fragment Length; D012703:Serotyping; D014774:Virulence / Q000235:genetics
chemical_list: D004269:DNA, Bacterial
keywords: Caracterización molecular; Carcasses; Escherichia coli O157; Meat products; Media res; Molecular characterization; Productos cárnicos