title: Direct DNA Extraction from Mycobacterium tuberculosis Frozen Stocks as a Reculture-Independent Approach to Whole-Genome Sequencing.
abstract: Culturing before DNA extraction represents a major time-consuming step in whole-genome sequencing of slow-growing bacteria, such as Mycobacterium tuberculosis. We report a workflow to extract DNA from frozen isolates without reculturing. Prepared libraries and sequence data were comparable with results from recultured aliquots of the same stocks.
authors.affiliation: Department of Epidemiology Research, Statens Serum Institut, Copenhagen, Denmark International Reference Laboratory of Mycobacteriology, Statens Serum Institut, Copenhagen, Denmark Greenland's Center of Health Research, Nuuk, Greenland kabm@ssi.dk.
authors.forename: K
authors.initials: K
authors.lastname: Bjorn-Mortensen
authors.affiliation: Molecular Mycobacteriology, Forschungszentrum Borstel, Leibniz-Zentrum für Medizin und Biowissenschaften, Borstel, Germany German Center for Infection Research (DZIF), Borstel, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Zallet
authors.affiliation: International Reference Laboratory of Mycobacteriology, Statens Serum Institut, Copenhagen, Denmark.
authors.forename: T
authors.initials: T
authors.lastname: Lillebaek
authors.affiliation: Department of Infectious Diseases, Copenhagen University Hospital and University of Southern Denmark, Odense, Denmark.
authors.forename: A B
authors.initials: AB
authors.lastname: Andersen
authors.affiliation: Molecular Mycobacteriology, Forschungszentrum Borstel, Leibniz-Zentrum für Medizin und Biowissenschaften, Borstel, Germany German Center for Infection Research (DZIF), Borstel, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Niemann
authors.affiliation: International Reference Laboratory of Mycobacteriology, Statens Serum Institut, Copenhagen, Denmark.
authors.forename: E M
authors.initials: EM
authors.lastname: Rasmussen
authors.affiliation: Molecular Mycobacteriology, Forschungszentrum Borstel, Leibniz-Zentrum für Medizin und Biowissenschaften, Borstel, Germany.
authors.forename: T A
authors.initials: TA
authors.lastname: Kohl
mesh_terms: D004269:DNA, Bacterial / Q000302:isolation & purification*; D005615:Freezing; D016680:Genome, Bacterial; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D011309:Preservation, Biological; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial