title: Three new cyathane diterpenoids from the medicinal fungus Cyathus africanus.
abstract: Three new cyathane diterpenoids, cyathin W (1), cyathin V (2), and cyathin T (3), were isolated from the solid culture of Cyathus africanus. The structures and configurations of these new compounds were elucidated on the basis of comprehensive spectroscopic analysis including 1D NMR, 2D NMR (HSQC, HMBC, NOESY), and HR-ESI-MS experiments. Compounds 1 and 3 showed moderate inhibition against nitric oxide production in lipopolysaccaride-activated macrophages with IC50 value of 80.07 and 88.87 μM, respectively. In cytotoxicity assay, compound 1 showed weak cytotoxicity against K562 cell line with IC50 value of 12.1 μM.
authors.affiliation: a State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences , Beijing 100101 , China.
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Han
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Jie-Kun
authors.initials: JK
authors.lastname: Xu
authors.forename: Li
authors.initials: L
authors.lastname: Bao
authors.forename: Feng
authors.initials: F
authors.lastname: Zhao
authors.forename: Yu-Hui
authors.initials: YH
authors.lastname: Chen
authors.forename: Wei-Ku
authors.initials: WK
authors.lastname: Zhang
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Liu
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D055365:Cyathus / Q000737:chemistry*; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D020014:K562 Cells; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000096:biosynthesis; D019906:Nuclear Magnetic Resonance, Biomolecular
chemical_list: D000970:Antineoplastic Agents; D004224:Diterpenes; D008070:Lipopolysaccharides; C497675:cyathane; C000601779:cyathin T; C000601778:cyathin V; C000601777:cyathin W; D009569:Nitric Oxide
keywords: Cyathus africanus; NO inhibition; Nidulariaceae; cyathane diterpenoids; cytotoxicity