title: Synthesis of PAMAM dendrimer-based fast cross-linking hydrogel for biofabrication.
abstract: Hydrogels possess great potential in biofabrication because they allow cell encapsulation and proliferation in a highly hydrated three-dimensional environment, and they provide biologically relevant chemical and physical signals. However, development of hydrogel systems that mimic the complexity of natural extracellular matrix remains a challenge. In this study, we report the development of a binary hydrogel system containing a synthetic poly(amido amine) (PAMAM) dendrimer and a natural polymer, i.e., hyaluronic acid (HA), to form a fast cross-linking hydrogel. Live cell staining experiment and cell viability assay of bone marrow stem cells demonstrated that cells were viable and proliferating in the in situ formed PAMAM/HA hydrogel system. Furthermore, introduction of a Arginylglycylaspartic acid (RGD) peptide into the hydrogel system significantly improved the cell viability, proliferation, and attachment. Therefore, this PAMAM/HA hydrogel system could be a promising platform for various applications in biofabrication.
authors.affiliation: a Department of Physical Sciences , Charleston Southern University , Charleston , SC , USA.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Bi
authors.forename: Jittima
authors.initials: J
authors.lastname: Amie Luckanagul
authors.forename: Ashley
authors.initials: A
authors.lastname: Allen
authors.forename: Matsepo
authors.initials: M
authors.lastname: Ramaboli
authors.forename: Emily
authors.initials: E
authors.lastname: Campbell
authors.forename: Davey
authors.initials: D
authors.lastname: West
authors.forename: Panita
authors.initials: P
authors.lastname: Maturavongsadit
authors.forename: Katelyn
authors.initials: K
authors.lastname: Brummett
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry* / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D060326:Chemistry Techniques, Synthetic; D050091:Dendrimers / Q000138:chemical synthesis* / Q000737:chemistry* / Q000494:pharmacology; D006820:Hyaluronic Acid / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects; D009842:Oligopeptides / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D050091:Dendrimers; D020100:Hydrogels; D009842:Oligopeptides; C104700:PAMAM Starburst; D013438:Sulfhydryl Compounds; C047981:arginyl-glycyl-aspartic acid; D006820:Hyaluronic Acid
keywords: PAMAM dendrimer; RGD peptide; biofabrication; bone marrow stem cells; hyaluronic acid; hydrogel