title: Effect of Avastin on the migration and invasion of pterygium fibroblasts.
abstract: OBJECTIVE
  To evaluate the effect of Avastin on human pterygium fibroblast migration and invasiveness.
  
  
  METHODS
  VEGF secretion was compared between human pterygium fibroblasts and conjunctival fibroblasts by measuring VEGF-A by ELISA. The influence of Avastin on HPF migration and invasiveness was observed by wound scratch and Transwell migration assays. The expression of p-ERK1/2 and p-FAK was analyzed by western blotting.
  
  
  RESULTS
  (1)VEGF was secreted in higher amounts by human pterygium fibroblasts than by conjunctival fibroblasts. (2) Avastin treatment decreased HPF migration and invasion. (3) Avastin significantly decreased the expression of p-ERK1/2 and p-FAK in human pterygium fibroblasts.
  
  
  CONCLUSIONS
  Avastin can inhibit migration and invasion of HPFs by decreasing the expression of p-ERK1/2 and p-FAK.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Xiangyin
authors.initials: X
authors.lastname: Sha
authors.forename: Ye
authors.initials: Y
authors.lastname: Wen
authors.forename: Wenting
authors.initials: W
authors.lastname: Zhao
authors.forename: Wei
authors.initials: W
authors.lastname: Luo
authors.forename: Zanmei
authors.initials: Z
authors.lastname: Hua
mesh_terms: D061067:Antibodies, Monoclonal, Humanized / Q000494:pharmacology*; D000068258:Bevacizumab; D015153:Blotting, Western; D054443:Cell Migration Assays; D002465:Cell Movement / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D003228:Conjunctiva / Q000166:cytology; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D051417:Focal Adhesion Kinase 1 / Q000378:metabolism*; D006801:Humans; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism*; D011625:Pterygium / Q000473:pathology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D061067:Antibodies, Monoclonal, Humanized; D042461:Vascular Endothelial Growth Factor A; D000068258:Bevacizumab; D051417:Focal Adhesion Kinase 1; C496050:PTK2 protein, human; D048052:Mitogen-Activated Protein Kinase 3