title: Surface Plasmon Resonance (SPR) for the Evaluation of Shear-Force-Dependent Bacterial Adhesion.
abstract: The colonization of Escherichia coli (E. coli) to host cell surfaces is known to be a glycan-specific process that can be modulated by shear stress. In this work we investigate whether flow rate changes in microchannels integrated on surface plasmon resonance (SPR) surfaces would allow for investigating such processes in an easy and high-throughput manner. We demonstrate that adhesion of uropathogenic E. coli UTI89 on heptyl α-d-mannopyranoside-modified gold SPR substrates is minimal under almost static conditions (flow rates of 10 µL·min⁻¹), and reaches a maximum at flow rates of 30 µL·min⁻¹ (≈30 mPa). This concept is applicable to the investigation of any ligand-pathogen interactions, offering a robust, easy, and fast method for screening adhesion characteristics of pathogens to ligand-modified interfaces.
authors.affiliation: Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR-CNRS 8520, Université Lille 1, Cité Scientifique, 59655 Villeneuve d'Ascq, France. morjakzzz@gmail.com.
authors.forename: Oleksandr
authors.initials: O
authors.lastname: Zagorodko
authors.affiliation: Unité de Glycobiologie Structurale et Fonctionnelle (UGSF), UMR8576 du CNRS, Université Lille 1, 59655 Villeneuve d'Ascq, France. julie.bouckaert@univ-lille1.fr.
authors.forename: Julie
authors.initials: J
authors.lastname: Bouckaert
authors.affiliation: Unité de Glycobiologie Structurale et Fonctionnelle (UGSF), UMR8576 du CNRS, Université Lille 1, 59655 Villeneuve d'Ascq, France. t.shkandina@gmail.com.
authors.forename: Tetiana
authors.initials: T
authors.lastname: Dumych
authors.affiliation: Institute of Cell Biology, National Academy of Sciences of Ukraine, 79005 Lviv, Ukraine. r.bilyy@gmail.com.
authors.forename: Rostyslav
authors.initials: R
authors.lastname: Bilyy
authors.affiliation: SENSIA SL, Poligono Aranguren, 9, Apdo. Correos 171, 20180 Oiartzun, Gipuzkoa, Spain. ilarroulet@seimcc.com.
authors.forename: Iban
authors.initials: I
authors.lastname: Larroulet
authors.affiliation: SENSIA SL, Poligono Aranguren, 9, Apdo. Correos 171, 20180 Oiartzun, Gipuzkoa, Spain. aritz_yanguas@hotmail.com.
authors.forename: Aritz
authors.initials: A
authors.lastname: Yanguas Serrano
authors.affiliation: LUNAM Université, CEISAM, UMR 6230 du CNRS, 2, rue de la Houssinière, BP 92208, 44322 Nantes Cedex 3, France. dimitri.alvarez-dorta@univ-nantes.fr.
authors.forename: Dimitri
authors.initials: D
authors.lastname: Alvarez Dorta
authors.affiliation: LUNAM Université, CEISAM, UMR 6230 du CNRS, 2, rue de la Houssinière, BP 92208, 44322 Nantes Cedex 3, France. Sebastien.Gouin@univ-nantes.fr.
authors.forename: Sebastien G
authors.initials: SG
authors.lastname: Gouin
authors.affiliation: Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR-CNRS 8520, Université Lille 1, Cité Scientifique, 59655 Villeneuve d'Ascq, France. florino@ping.ro.|Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1-7 Gheorghe Polizu, 011061 Bucharest, Romania. florino@ping.ro.|National R&D Institute for Chemistry and Petrochemistry ICECHIM, 202 Splaiul Independentei, 060021 Bucharest, Romania. florino@ping.ro.
authors.forename: Stefan-Ovidiu
authors.initials: SO
authors.lastname: Dima
authors.affiliation: National R&D Institute for Chemistry and Petrochemistry ICECHIM, 202 Splaiul Independentei, 060021 Bucharest, Romania. florino@ping.ro.
authors.forename: Florin
authors.initials: F
authors.lastname: Oancea
authors.affiliation: Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR-CNRS 8520, Université Lille 1, Cité Scientifique, 59655 Villeneuve d'Ascq, France. rabah.boukherroub@univ-lille1.fr.
authors.forename: Rabah
authors.initials: R
authors.lastname: Boukherroub
authors.affiliation: Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR-CNRS 8520, Université Lille 1, Cité Scientifique, 59655 Villeneuve d'Ascq, France. sabine.szunerits@iri.univ-lille1.fr.
authors.forename: Sabine
authors.initials: S
authors.lastname: Szunerits
mesh_terms: D001422:Bacterial Adhesion; D001696:Biomechanical Phenomena; D015374:Biosensing Techniques / Q000379:methods; D004926:Escherichia coli / Q000502:physiology*; D006046:Gold / Q000737:chemistry; D008358:Mannose / Q000737:chemistry; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D006046:Gold; D008358:Mannose
keywords: Escherichia coli (E. coli); carbohydrates; flow rate; shear force enhancement; surface plasmon resonance (SPR)