title: [Role of CTHRC1 in proliferation, migration and invasion of human colorectal cancer cells].
abstract: OBJECTIVE
  To explore the expression of collagen triple helix repeat containing 1 (CTHRC1) in colorectal cancer and study its role in regulating the biological behaviors of colorectal cancer LoVo cells in vitro.
  
  
  METHODS
  Real-time PCR and Western blotting were used to detect the expressions of CTHRC1 in colorectal cancer tissue and paired adjacent nontumorous tissue and in 5 colorectal cancer cells. pGPU6-CTHRC1-shRNA was transfected into LoVo cells and the changes in cell proliferation was assessed using cell counting kit-8 (CCK8) assay; the changes in cell migration and invasion were investigated using Transwell assay; plate colony forming test was used to evaluate the adhesion and colony forming activity of the cells. Western blotting was used to analyze the changes in the expressions of the related pathway markers.
  
  
  RESULTS
  The relative expression of CTHRC1 mRNA in the cancer tissue specimens was 0.0411∓0.054, significantly higher than that in the adjacent tissues (P=0.016); this result was consistent with that of the protein assay. SW620 and LoVo cells showed obviously higher expressions of CTHRC1 than HT29 and SW480 cells at both mRNA and protein levels. LoVo cells transfected with CTHRC1 shRNA exhibited significantly suppressed proliferation, migration, invasion and colony-forming ability (P<0.05) and lowered expression of phosphorylated ERK1/2 (P-ERK1/2), but the expression of total ERK1/2 showed no obvious changes. CTHRC1 inhibition caused reverse epithelial-mesenchymal transition LoVo cells shown by increased E-cadherin expression and decreased expressions of N-cadherin, vimentin, and β-catenin.
  
  
  CONCLUSIONS
  CTHRC1 is up-regulated in colorectal cancer tissues and SW620 and LoVo cells to promote the cell proliferation, migration, invasion and colony formation. CTHRC1 can enhance epithelial-mesenchymal transition of colorectal cancer cells by activating ERK1/2 to promote tumor cell metastasis and invasion.
authors.affiliation: Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.E-mail: yishu_4839@126.com.
authors.forename: Li
authors.initials: L
authors.lastname: Yan
authors.forename: Geng-Tai
authors.initials: GT
authors.lastname: Ye
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Shen
authors.forename: Xianjun
authors.initials: X
authors.lastname: Zhu
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
authors.forename: Guoxin
authors.initials: G
authors.lastname: Li
mesh_terms: D015820:Cadherins / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology; D058750:Epithelial-Mesenchymal Transition; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D006801:Humans; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection; D014746:Vimentin / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism
chemical_list: C502426:CTHRC1 protein, human; D015820:Cadherins; D016326:Extracellular Matrix Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D014746:Vimentin; D051176:beta Catenin