title: [Effect of Bacillus cereus hemolysin II on hepatocyte cells].
abstract: We investigated the efficiency of increasing the permeability (permeabilization) of cell membranes in primary liver cells by Bacillus cereus hemolysin II. An assessment of the degree of permeabilization was car ried out by measuring the fluorescence intensity of various low molecular weight dyes, which enter through pores into hepatocyte cells cultivated with hemolysin. We uncovered a high efficacy of hemolysin HlyII action on hepatocyte cell walls, which exceeded the effect of nonionic detergent, digitonin, which is commonly employed for pore formation in various cell membranes. Our results also point to the reversibility of membrane permeabilization in primary hepatocytes. The data obtained in this study can be utilized for assessments of pore-forming activity, in studies of hepatic mechanisms of action, and also the determination of the liver toxicity for different low molecular weight drugs.
authors.forename: O A
authors.initials: OA
authors.lastname: Kholodkov
authors.forename: Zh
authors.initials: Zh
authors.lastname: Budarina
authors.forename: J I
authors.initials: JI
authors.lastname: Kovalevskaya
authors.forename: A V
authors.initials: AV
authors.lastname: Si'unov
authors.forename: A
authors.initials: A
authors.lastname: Solonin
mesh_terms: D001409:Bacillus cereus / Q000737:chemistry*; D001426:Bacterial Proteins / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects; D002463:Cell Membrane Permeability / Q000187:drug effects; D002473:Cell Wall / Q000187:drug effects; D002478:Cells, Cultured; D004072:Digitonin / Q000494:pharmacology; D006460:Hemolysin Proteins / Q000494:pharmacology*; D022781:Hepatocytes / Q000187:drug effects*; D006801:Humans
chemical_list: C085361:ApxII toxin, bacteria; D001426:Bacterial Proteins; D006460:Hemolysin Proteins; D004072:Digitonin