title: [Effect of capsaicin on intestinal permeation of P-glycoprotein substrate rhodamine 123 and fluorescein sodium in rats].
abstract: OBJECTIVE
  To investigate the role of capsaicin in regulating permeation of P-gp substrate rhodamine 123 (R123) across the jejunum, ileum and colon membranes of rats.
  
  
  METHODS
  The permeability of R123 or fluorescein sodium (CF) across the jejunum, ileum and colon membranes of male SD rats was evaluated using a Ussing chamber. The concentration of R123 or CF in the receptor was determined using fluorospectrophotometry to calculate the apparent permeability coefficient (Papp).
  
  
  RESULTS
  Compared with the blank control group, capsaicin increased the permeability of R123 across jejunal membranes in the mucosal-to-serosal (M-S) direction and decreased its permeability in the serosal-to-mucosal (S-M) direction, but produced no obvious effect on R123 transport across the ileum or colon membranes. Capsaicin caused a regional increase in the permeability of CF across the jejunal membranes compared with the control group, but CF transport across the ileum and colon membranes was not affected.
  
  
  CONCLUSIONS
  Capsaicin can affect the transport of R123 and CF across rat jejunal membranes, and this effect is shows an obvious intestine segment-related difference probably because of the different distribution of P-gp or tight junction in the intestines. This finding suggests that capsaicin is a weak P-gp inhibitor and an improver of mucous membrane channels.
authors.affiliation: Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.E-mail: qianyinglny@163.com.
authors.forename: Qianying
authors.initials: Q
authors.lastname: Liang
authors.forename: Lian
authors.initials: L
authors.lastname: Duan
authors.forename: Zhiquan
authors.initials: Z
authors.lastname: Zhuang
authors.forename: Boxin
authors.initials: B
authors.lastname: Zhao
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.forename: Shengqi
authors.initials: S
authors.lastname: Wang
authors.forename: Fuheng
authors.initials: F
authors.lastname: Yang
authors.forename: Sijia
authors.initials: S
authors.lastname: Liu
authors.forename: Guofeng
authors.initials: G
authors.lastname: Li
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism*; D000818:Animals; D002211:Capsaicin / Q000494:pharmacology*; D003106:Colon / Q000378:metabolism; D019793:Fluorescein / Q000493:pharmacokinetics*; D007082:Ileum / Q000378:metabolism; D007408:Intestinal Absorption; D007583:Jejunum / Q000378:metabolism; D008297:Male; D010539:Permeability; D051381:Rats; D017207:Rats, Sprague-Dawley; D020112:Rhodamine 123 / Q000493:pharmacokinetics*
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D020112:Rhodamine 123; D002211:Capsaicin; D019793:Fluorescein