title: On-Demand Lensless Single Cell Imaging Activated by Differential Resistive Pulse Sensing.
abstract: We present an on-demand single cell imaging technique activated by differential resistive pulse sensing in a portable system integrating a microfluidic differential coulter counter and a lensless complementary metal-oxide-semiconductor (CMOS) imaging sensor. Dual parametric single cell analysis and on-demand single cell imaging have been demonstrated by microbeads of different sizes and a cell mixture including red blood cells (RBCs) and tumor cell line HepG2 cells. The on-demand imaging capability could avoid generating useless images without cells and enable selective imaging of single cells within a specific size range.
authors.affiliation: †Pillar of Engineering Product Development, Singapore University of Technology and Design, Singapore 487372, Singapore.
authors.forename: Jinhong
authors.initials: J
authors.lastname: Guo
authors.affiliation: ‡School of Electronics and Information, Hangzhou Dianzi University, Hangzhou 310018, P. R. China.
authors.forename: Xiwei
authors.initials: X
authors.lastname: Huang
authors.affiliation: †Pillar of Engineering Product Development, Singapore University of Technology and Design, Singapore 487372, Singapore.
authors.forename: Ye
authors.initials: Y
authors.lastname: Ai
mesh_terms: D002460:Cell Line; D006801:Humans; D059010:Single-Cell Analysis