title: Evaluation of different methods for immobilization of Candida antarctica lipase B (CalB lipase) in polyurethane foam and its application in the production of geranyl propionate.
abstract: With the aim of studying the best method for the interaction of polyurethane (PU) foam and Candida antarctica lipase B, different methods of CalB immobilization were studied: adsorption (PU-ADS), bond (using polyethyleneimine) (PU-PEI), ionic adsorption by PEI with cross-linking with glutaraldehyde (PU-PEI-GA) and entrapment (PU). The characterization of immobilized enzyme derivatives was performed by apparent density and Fourier transform infrared spectroscopy. The free enzyme and enzyme preparations were evaluated at different pH values and temperatures. The highest enzyme activity was obtained using the PU method (5.52 U/g). The methods that stood out to compare the stabilities and kinetic parameters were the PU and PU-ADS. Conversions of 83.5 and 95.9 % for PU and PU-ADS derivatives were obtained, in 24 h reaction, using citronella oil and propionic acid as substrates.
authors.affiliation: Chemical and Food Engineering Department, Federal University of Santa Catarina (UFSC), PO Box 476, Florianópolis, SC, 88040-900, Brazil.
authors.forename: Gabrieli
authors.initials: G
authors.lastname: Nicoletti
authors.forename: Eliane P
authors.initials: EP
authors.lastname: Cipolatti
authors.forename: Alexsandra
authors.initials: A
authors.lastname: Valério
authors.forename: NatáliaThaisa Gamba
authors.initials: NG
authors.lastname: Carbonera
authors.forename: Nicole Spillere
authors.initials: NS
authors.lastname: Soares
authors.forename: Eron
authors.initials: E
authors.lastname: Theilacker
authors.forename: Jorge L
authors.initials: JL
authors.lastname: Ninow
authors.forename: Débora
authors.initials: D
authors.lastname: de Oliveira
mesh_terms: D000327:Adsorption; D003432:Cross-Linking Reagents / Q000737:chemistry; D004789:Enzyme Activation; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry; D005421:Flavoring Agents / Q000138:chemical synthesis*; D005656:Fungal Proteins / Q000737:chemistry*; D005740:Gases / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry*; D011140:Polyurethanes / Q000737:chemistry*; D011422:Propionates / Q000138:chemical synthesis* / Q000737:chemistry; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D005421:Flavoring Agents; D005656:Fungal Proteins; D005740:Gases; D010938:Plant Oils; D011140:Polyurethanes; D011422:Propionates; D008049:Lipase; C431349:lipase B, Candida antarctica; C029658:propionic acid; C076730:citronella oil