title: Specific phosphoantibodies reveal two phosphorylation sites in yeast Pma1 in response to glucose.
abstract: Glucose triggers post-translational modifications of the Saccharomyces cerevisiae plasma membrane H(+)-ATPase (Pma1) that lead to an increase in enzyme activity. The activation results from changes in two kinetic parameters: an increase in the affinity of the enzyme for ATP, depending on Ser899, and an increase in the Vmax involving Ser911/Thr912. Using phosphospecific antibodies, we show that Ser899 and Ser911/Thr912 are phosphorylated in vivo during glucose activation and that protein phosphatase Glc7 is involved in the dephosphorylation of Ser899 upon glucose starvation.
authors.affiliation: Departamento de Bioquímica and Instituto de Investigaciones Biomédicas 'Alberto Sols', Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid, Arturo Duperier, 4, 28029 Madrid, Spain.
authors.forename: María J
authors.initials: MJ
authors.lastname: Mazón
authors.affiliation: Departamento de Bioquímica and Instituto de Investigaciones Biomédicas 'Alberto Sols', Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid, Arturo Duperier, 4, 28029 Madrid, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Eraso
authors.affiliation: Departamento de Bioquímica and Instituto de Investigaciones Biomédicas 'Alberto Sols', Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid, Arturo Duperier, 4, 28029 Madrid, Spain fportillo@iib.uam.es.
authors.forename: Francisco
authors.initials: F
authors.lastname: Portillo
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000906:Antibodies / Q000276:immunology; D004789:Enzyme Activation; D005947:Glucose / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D010766:Phosphorylation; D054645:Protein Phosphatase 1 / Q000235:genetics / Q000378:metabolism*; D011499:Protein Processing, Post-Translational / Q000235:genetics; D006180:Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000906:Antibodies; D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; D000255:Adenosine Triphosphate; C092048:GLC7 protein, S cerevisiae; D054645:Protein Phosphatase 1; C092477:PMA1 protein, S cerevisiae; D006180:Proton-Translocating ATPases; D005947:Glucose
keywords: Pma1; glucose activation; phosphorylation; yeast