title: Body fluid matrix evaluation on a Roche cobas 8000 system.
abstract: OBJECTIVE
  Chemical analysis of body fluids is commonly requested by physicians. Because most commercial FDA-cleared clinical laboratory assays are not validated by diagnostic manufacturers for "non-serum" and "non-plasma" specimens, laboratories may need to complete additional validation studies to comply with regulatory requirements regarding body fluid testing. The objective of this report is to perform recovery studies to evaluate potential body fluid matrix interferences for commonly requested chemistry analytes.
  
  
  METHODS
  Using an IRB-approved protocol, previously collected clinical body fluid specimens (biliary/hepatic, cerebrospinal, dialysate, drain, pancreatic, pericardial, peritoneal, pleural, synovial, and vitreous) were de-identified and frozen (-20°C) until experiments were performed. Recovery studies (spiking with high concentration serum, control, and/or calibrator) were conducted using 10% spiking solution by volume; n=5 specimens per analyte/body fluid investigated. Specimens were tested on a Roche cobas 8000 system (c502, c702, e602, and ISE modules).
  
  
  RESULTS
  In all 80 analyte/body fluid combinations investigated (including amylase, total bilirubin, urea nitrogen, carbohydrate antigen 19-9, carcinoembryonic antigen, cholesterol, chloride, creatinine, glucose, potassium, lactate dehydrogenase, lipase, rheumatoid factor, sodium, total protein, triglycerides, and uric acid), the average percent recovery was within predefined acceptable limits (less than ±10% from the calculated ideal recovery).
  
  
  CONCLUSIONS
  The present study provides evidence against the presence of any systematic matrix interference in the analyte/body fluid combinations investigated on the Roche cobas 8000 system. Such findings support the utility of ongoing body fluid validation initiatives conducted to maintain compliance with regulatory requirements.
authors.affiliation: ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT 84108, USA.
authors.forename: William E
authors.initials: WE
authors.lastname: Owen
authors.affiliation: ARUP Laboratories, 500 Chipeta Way, Salt Lake City, UT 84108, USA.
authors.forename: Mindy L
authors.initials: ML
authors.lastname: Thatcher
authors.affiliation: ARUP Laboratories, 500 Chipeta Way, Salt Lake City, UT 84108, USA.
authors.forename: Karolyn J
authors.initials: KJ
authors.lastname: Crabtree
authors.affiliation: ARUP Laboratories, 500 Chipeta Way, Salt Lake City, UT 84108, USA.
authors.forename: Ryan W
authors.initials: RW
authors.lastname: Greer
authors.affiliation: ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT 84108, USA; Department of Pathology, 15 North Medical Drive East, Salt Lake City, UT 84112, USA.
authors.forename: Frederick G
authors.initials: FG
authors.lastname: Strathmann
authors.affiliation: ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT 84108, USA; Department of Pathology, 15 North Medical Drive East, Salt Lake City, UT 84112, USA.
authors.forename: Joely A
authors.initials: JA
authors.lastname: Straseski
authors.affiliation: ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT 84108, USA; Department of Pathology, 15 North Medical Drive East, Salt Lake City, UT 84112, USA. Electronic address: jonathan.genzen@path.utah.edu.
authors.forename: Jonathan R
authors.initials: JR
authors.lastname: Genzen
mesh_terms: D001826:Body Fluids / Q000378:metabolism*; D002624:Chemistry, Clinical / Q000379:methods*; D006801:Humans; D010994:Pleura / Q000378:metabolism
keywords: Assay interference; Body fluid; Matrix effect; Method validation; Recovery study; Spiking experiment