title: Quercetin supplementation does not enhance cerebellar mitochondrial biogenesis and oxidative status in exercised rats.
abstract: The present study tested the hypothesis that quercetin may inhibit the mitochondrial and antioxidant adaptations induced by exercise in cerebellar tissue. Thirty-five 6-week-old Wistar rats were randomly allocated into the following groups: quercetin, exercised (Q-Ex; n = 9); quercetin, sedentary (Q-Sed; n = 9); no quercetin, exercised (NQ-Ex; n = 9); and no quercetin, sedentary (NQ-Sed; n = 8). After 6 weeks of quercetin supplementation and/or exercise training, cerebellums were collected. Protein carbonyl content (PCC), sirtuin 1, peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α), messenger RNA levels, citrate synthase (CS), and mitochondrial DNA were measured. When Q-Sed was compared with NQ-Sed, PCC (P < .005) showed decreased levels, whereas PGC-1α, sirtuin 1 (both, P < .01), mitochondrial DNA (P < .001), and CS (P < .01) increased. However, when Q-Ex was compared with Q-Sed, PCC showed increased levels (P < .001), whereas CS decreased (P < .01). Furthermore, the NQ-Ex group experienced an increase in PGC-1α messenger RNA levels in comparison with NQ-Sed (P > .01). This effect, however, did not appear in Q-Ex (P < .05). Therefore, we must hypothesize that either the dose (25 mg/kg) or the length of the quercetin supplementation period that was used in the present study (or perhaps both) may impair exercise-induced adaptations in cerebellar tissue.
authors.affiliation: Department of Health Sciences, University of Jaén, E-23071 Jaén, Spain. Electronic address: casusopt@gmail.com.
authors.forename: Rafael A
authors.initials: RA
authors.lastname: Casuso
authors.affiliation: Department of Health Sciences, University of Jaén, E-23071 Jaén, Spain. Electronic address: amamat@gmail.com.
authors.forename: Antonio
authors.initials: A
authors.lastname: Martínez-Amat
authors.affiliation: Department of Health Sciences, University of Jaén, E-23071 Jaén, Spain. Electronic address: fhita@ujaen.es.
authors.forename: Fidel
authors.initials: F
authors.lastname: Hita-Contreras
authors.affiliation: Department of Physiology, Faculty of Pharmacy and Institute of Nutrition and Food Technology, Faculty of Sport Sciences, and Institute of Nutrition and Food Technology, University of Granada, Campus Universitario de Cartuja s/n, Granada 18071, Spain. Electronic address: dcamiletti@ugr.es.
authors.forename: Daniel
authors.initials: D
authors.lastname: Camiletti-Moirón
authors.affiliation: Department of Physiology, Faculty of Pharmacy and Institute of Nutrition and Food Technology, Faculty of Sport Sciences, and Institute of Nutrition and Food Technology, University of Granada, Campus Universitario de Cartuja s/n, Granada 18071, Spain. Electronic address: paranda@ugr.es.
authors.forename: Pilar
authors.initials: P
authors.lastname: Aranda
authors.affiliation: Department of Music, Plastic Expression and Body Language, University of Jaén, E-23071 Jaén, Spain. Electronic address: emilioml@ujaen.es.
authors.forename: Emilio
authors.initials: E
authors.lastname: Martínez-López
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002531:Cerebellum / Q000187:drug effects* / Q000378:metabolism; D002950:Citrate (si)-Synthase / Q000378:metabolism; D004272:DNA, Mitochondrial / Q000187:drug effects; D019587:Dietary Supplements; D008297:Male; D008928:Mitochondria / Q000235:genetics; D001678:Organelle Biogenesis; D018384:Oxidative Stress / Q000187:drug effects*; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D010805:Physical Conditioning, Animal / Q000502:physiology*; D050050:Protein Carbonylation / Q000187:drug effects; D011794:Quercetin / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D017208:Rats, Wistar; D056564:Sirtuin 1 / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D000975:Antioxidants; D004272:DNA, Mitochondrial; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; C501429:Ppargc1a protein, rat; D012333:RNA, Messenger; D014157:Transcription Factors; D011794:Quercetin; D002950:Citrate (si)-Synthase; D056564:Sirtuin 1
keywords: Flavonoids; Oxidative stress; PGC-1α; SIRT1; Training; Wistar rats