title: Argentation high performance liquid chromatography on-line coupled to gas chromatography for the analysis of monounsaturated polyolefin oligomers in packaging materials and foods.
abstract: Multidimensional chromatography based on two-dimensional high performance liquid chromatography on-line coupled to gas chromatography (on-line HPLC-HPLC-GC) enables the separate analysis of saturated, monounsaturated and aromatic hydrocarbons in packaging materials like polyolefins or paperboard and their migrates into foods. Since normal-phase HPLC on silica gel did not preseparate saturated from monounsaturated hydrocarbons, a separation step on a normal-phase HPLC column treated in the laboratory with an optimized amount of silver nitrate was added. The preparation of this HPLC column and the instrumental set-up are described, followed by examples showing the potential of the method. In a preliminary investigation of 11 polyolefin granulates for food contact up to 40% monounsaturated hydrocarbons among the oligomers C16-35 were determined.
authors.affiliation: Technische Universität Dresden, Chair of Food Science and Food Contact Materials, D-01069 Dresden, Germany; Official Food Control Authority of the Canton of Zurich, CH-8032 Zurich, Switzerland.
authors.forename: Martin
authors.initials: M
authors.lastname: Lommatzsch
authors.affiliation: Official Food Control Authority of the Canton of Zurich, CH-8032 Zurich, Switzerland.
authors.forename: Maurus
authors.initials: M
authors.lastname: Biedermann
authors.affiliation: Technische Universität Dresden, Chair of Food Science and Food Contact Materials, D-01069 Dresden, Germany. Electronic address: thomas.simat@tu-dresden.de.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Simat
authors.affiliation: Official Food Control Authority of the Canton of Zurich, CH-8032 Zurich, Switzerland.
authors.forename: Koni
authors.initials: K
authors.lastname: Grob
mesh_terms: D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D018857:Food Packaging; D006838:Hydrocarbons / Q000032:analysis; D006841:Hydrocarbons, Aromatic / Q000032:analysis; D011090:Polyenes / Q000032:analysis; D058428:Silica Gel
chemical_list: D006838:Hydrocarbons; D006841:Hydrocarbons, Aromatic; D011090:Polyenes; D058428:Silica Gel; C035051:PL 732
keywords: Argentation HPLC; Comprehensive two-dimensional GC (GC×GC); Food contact materials; On-line HPLC–GC–FID; Polyolefin oligomers; Silver-impregnated HPLC column