title: Construction and evaluation of nitric oxide generating vascular graft material loaded with organoselenium catalyst via layer-by-layer self-assembly.
abstract: A new biomimetic material for artificial blood vessel with in situ catalytic generation of nitric oxide (NO) was prepared in this study. Organoselenium immobilized polyethyleneimine as NO donor catalyst and sodium alginate were alternately loaded onto the surface of electrospun polycaprolactone matrix via electrostatic layer-by-layer self-assembly. This material revealed significant NO generation when contacting NO donor S-nitrosoglutathione (GSNO). Adhesion and spreading of smooth muscle cells were inhibited on this material in the presence of GSNO, while proliferation of endothelial cells was promoted. In vitro platelet adhesion and arteriovenous shunt experiments demonstrated good antithrombotic properties of this material, with inhibited platelet activation and aggregation, and prevention of acute thrombosis. This study may provide a new method of improving cellular function and antithrombotic property of vascular grafts.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials for Ministry of Education, College of Life Sciences, Nankai University, Tianjin, 300071, China.
authors.forename: Jun
authors.initials: J
authors.lastname: An
authors.forename: SiYuan
authors.initials: S
authors.lastname: Chen
authors.forename: JingChen
authors.initials: J
authors.lastname: Gao
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
authors.forename: YuanYuan
authors.initials: Y
authors.lastname: Wang
authors.forename: YanDong
authors.initials: Y
authors.lastname: Li
authors.forename: Sergey
authors.initials: S
authors.lastname: Mikhalovsky
authors.forename: DeLing
authors.initials: D
authors.lastname: Kong
authors.forename: ShuFang
authors.initials: S
authors.lastname: Wang
mesh_terms: D016475:3T3 Cells; D000464:Alginates / Q000737:chemistry; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D001807:Blood Vessel Prosthesis; D002384:Catalysis; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D051379:Mice; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D032389:Myocytes, Smooth Muscle / Q000187:drug effects; D009569:Nitric Oxide / Q000378:metabolism*; D016566:Organoselenium Compounds / Q000737:chemistry*; D015539:Platelet Activation / Q000187:drug effects; D010973:Platelet Adhesiveness / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects; D011091:Polyesters / Q000737:chemistry; D011094:Polyethyleneimine / Q000737:chemistry; D026422:S-Nitrosoglutathione / Q000378:metabolism*; D013499:Surface Properties; D013927:Thrombosis / Q000517:prevention & control
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D006603:Hexuronic Acids; D016566:Organoselenium Compounds; D011091:Polyesters; C016240:polycaprolactone; D009569:Nitric Oxide; D026422:S-Nitrosoglutathione; D020723:Glucuronic Acid; D011094:Polyethyleneimine