title: Human adipose-derived mesenchymal progenitor cells engraft into rabbit articular cartilage.
abstract: Mesenchymal stem cells (MSCs) are known to have the potential for articular cartilage regeneration, and are suggested for the treatment of osteoarthritis (OA). Here, we investigated whether intra-articular injection of xenogeneic human adipose-derived mesenchymal progenitor cells (haMPCs) promoted articular cartilage repair in rabbit OA model and engrafted into rabbit articular cartilage. The haMPCs were cultured in vitro, and phenotypes and differentiation characteristics of cells were evaluated. OA was induced surgically by anterior cruciate ligament transection (ACLT) and medical meniscectomy of knee joints. At six weeks following surgery, hyaluronic acid (HA) or haMPCs was injected into the knee joints, the contralateral knee served as normal control. All animals were sacrificed at the 16th week post-surgery. Assessments were carried out by macroscopic examination, hematoxylin/eosin (HE) and Safranin-O/Fast green stainings and immunohistochemistry. The data showed that haMPC treatment promoted cartilage repair. Signals of human mitochondrial can be directly detected in haMPC treated cartilage. The haMPCs expressed human leukocyte antigen I (HLA-I) but not HLA-II-DR in vivo. These results suggest that intra-articular injection of haMPCs promotes regeneration of articular cartilage in rabbit OA model, and support the notion that MPCs are transplantable between HLA-incompatible individuals.
authors.affiliation: Cellular Biomedicine Group, Palo Alto, CA 94301, USA. maxwell.wang@cellbiomedgroup.com.
authors.forename: Wen
authors.initials: W
authors.lastname: Wang
authors.affiliation: Cellular Biomedicine Group, Palo Alto, CA 94301, USA. elena.he@cellbiomedgroup.com.
authors.forename: Na
authors.initials: N
authors.lastname: He
authors.affiliation: Cellular Biomedicine Group, Palo Alto, CA 94301, USA. iris.feng@cellbiomedgroup.com.
authors.forename: Chenchen
authors.initials: C
authors.lastname: Feng
authors.affiliation: Cellular Biomedicine Group, Palo Alto, CA 94301, USA. victor.liu@cellbiomedgroup.com.
authors.forename: Victor
authors.initials: V
authors.lastname: Liu
authors.affiliation: Cellular Biomedicine Group, Palo Alto, CA 94301, USA. lizzy.zhang@cellbiomedgroup.com.
authors.forename: Luyi
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Cellular Biomedicine Group, Palo Alto, CA 94301, USA. richard.wang@cellbiomedgroup.com.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.affiliation: Cellular Biomedicine Group, Palo Alto, CA 94301, USA. jeffrey.he@cellbiomedgroup.com.
authors.forename: Jiaping
authors.initials: J
authors.lastname: He
authors.affiliation: Department of Pathology, Shanghai Medical College, Fudan University, Shanghai 200433, China. zhutengfang@shmu.edu.cn.
authors.forename: Tengfang
authors.initials: T
authors.lastname: Zhu
authors.affiliation: Department of Pathology, Shanghai Medical College, Fudan University, Shanghai 200433, China. wangshuyang@shmu.edu.cn.
authors.forename: Shuyang
authors.initials: S
authors.lastname: Wang
authors.affiliation: Animal facility, Shanghai Medical College, Fudan University, Shanghai 200433, China. qiaoweiwei@shmu.edu.cn.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Qiao
authors.affiliation: Cellular Biomedicine Group, Palo Alto, CA 94301, USA. maggie.li@cellbiomedgroup.com.
authors.forename: Suke
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China. guangdongzhou@126.com.
authors.forename: Guangdong
authors.initials: G
authors.lastname: Zhou
authors.affiliation: Cellular Biomedicine Group, Palo Alto, CA 94301, USA. lizzy.zhang@cellbiomedgroup.com.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Cellular Biomedicine Group, Palo Alto, CA 94301, USA. chase.dai@cellbiomedgroup.com.
authors.forename: Chengxiang
authors.initials: C
authors.lastname: Dai
authors.affiliation: Cellular Biomedicine Group, Palo Alto, CA 94301, USA. william.cao@cellbiomedgroup.com.|Department of Histology and Embryology, Shanghai Medical College, Fudan University, Shanghai 200433, China. william.cao@cellbiomedgroup.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Cao
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D001169:Arthritis, Experimental / Q000473:pathology / Q000628:therapy*; D002478:Cells, Cultured; D006648:Histocompatibility; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D006801:Humans; D006820:Hyaluronic Acid / Q000008:administration & dosage; D007270:Injections, Intra-Articular; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000502:physiology*; D020370:Osteoarthritis, Knee / Q000628:therapy*; D011817:Rabbits
chemical_list: D015395:Histocompatibility Antigens Class I; D006820:Hyaluronic Acid
keywords: human adipose-derived mesenchymal progenitor cells; human leukocyte antigen; mesenchymal stem cells; osteoarthritis