title: Allyl isothiocyanate induces stomatal closure in Vicia faba.
abstract: Isothiocyanates are enzymatically produced from glucosinolates in plants, and allyl isothiocyanate (AITC) induces stomatal closure in Arabidopsis thaliana. In this study, we investigated stomatal responses to AITC in Vicia faba. AITC-induced stomatal closure accompanied by reactive oxygen species (ROS) and NO production, cytosolic alkalization and glutathione (GSH) depletion in V. faba. GSH monoethyl ester induced stomatal reopening and suppressed AITC-induced GSH depletion in guard cells. Exogenous catalase and a peroxidase inhibitor, salicylhydroxamic acid, inhibited AITC-induced stomatal closure, unlike an NAD(P)H oxidase inhibitor, diphenylene iodonium chloride. The peroxidase inhibitor also abolished the AITC-induced ROS production, NO production, and cytosolic alkalization. AITC-induced stomatal closure was suppressed by an NO scavenger, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide, and an agent to acidify cytosol, butyrate. These results indicate that AITC-induced stomatal closure in V. faba as well as in A. thaliana and suggest that AITC signaling in guard cells is conserved in both plants.
authors.affiliation: a Graduate School of Environmental and Life Science , Okayama University , Okayama , Japan.
authors.forename: Muhammad Abdus
authors.initials: MA
authors.lastname: Sobahan
authors.forename: Nasima
authors.initials: N
authors.lastname: Akter
authors.forename: Eiji
authors.initials: E
authors.lastname: Okuma
authors.forename: Misugi
authors.initials: M
authors.lastname: Uraji
authors.forename: Wenxiu
authors.initials: W
authors.lastname: Ye
authors.forename: Izumi C
authors.initials: IC
authors.lastname: Mori
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Murata
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000378:metabolism; D001565:Benzoates / Q000494:pharmacology; D020148:Butyric Acid / Q000494:pharmacology; D002374:Catalase / Q000037:antagonists & inhibitors / Q000378:metabolism; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology; D005978:Glutathione / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D007093:Imidazoles / Q000494:pharmacology; D017879:Isothiocyanates / Q000494:pharmacology*; D019255:NADPH Oxidases / Q000037:antagonists & inhibitors / Q000378:metabolism; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000378:metabolism; D009861:Onium Compounds / Q000494:pharmacology; D009195:Peroxidase / Q000037:antagonists & inhibitors / Q000378:metabolism; D054046:Plant Stomata / Q000187:drug effects* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism; D012457:Salicylamides / Q000494:pharmacology; D015398:Signal Transduction; D031307:Vicia faba / Q000187:drug effects* / Q000378:metabolism
chemical_list: D001565:Benzoates; D016166:Free Radical Scavengers; D007093:Imidazoles; D017879:Isothiocyanates; D009861:Onium Compounds; D017382:Reactive Oxygen Species; D012457:Salicylamides; D020148:Butyric Acid; C079393:1,3-dihydroxy-4,4,5,5-tetramethyl-2-(4-carboxyphenyl)tetrahydroimidazole; D009569:Nitric Oxide; C007517:diphenyleneiodonium; C042431:S-ethyl glutathione; C005703:salicylhydroxamic acid; C004471:allyl isothiocyanate; D002374:Catalase; D009195:Peroxidase; D019255:NADPH Oxidases; D005978:Glutathione
keywords: guard cells; peroxidase; signaling; stomatal closure