title: Determination of ketosteroid hormones in meat by liquid chromatography tandem mass spectrometry and derivatization chemistry.
abstract: A method for the determination and quantification of ketosteroid hormones in meat by mass spectrometry, based on the derivatization of the carbonyl moiety of steroids by O-methylhydroxylamine, is presented. The quantitative assay is performed by means of multiple-reaction-monitoring (MRM) scan mode and using the corresponding labelled species, obtained by reaction with d 3-methoxylamine, as internal standard. The accuracy of the method was established by evaluating artificially spiked samples, obtaining values in the range 90-110%. Recovery tests were performed on blank matrix samples spiked with non-natural steroids including trenbolone and melengestrol acetate. The latter experiment revealed that the yield of the extraction processes was approximately 60%. Good values of LOQ and LOD were achieved, making this method competitive with current hormone assay methods.
authors.affiliation: Dipartimento di Chimica e Tecnologie Chimiche, Università della Calabria, Via P. Bucci Cubo 12/C, 87036, Arcavacata di Rende, CS, Italy.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Di Donna
authors.forename: Hicham
authors.initials: H
authors.lastname: Benabdelkamel
authors.forename: Domenico
authors.initials: D
authors.lastname: Taverna
authors.forename: Serena
authors.initials: S
authors.lastname: Indelicato
authors.forename: Donatella
authors.initials: D
authors.lastname: Aiello
authors.forename: Anna
authors.initials: A
authors.lastname: Napoli
authors.forename: Giovanni
authors.initials: G
authors.lastname: Sindona
authors.forename: Fabio
authors.initials: F
authors.lastname: Mazzotti
mesh_terms: D007664:Ketosteroids / Q000032:analysis* / Q000302:isolation & purification; D008460:Meat / Q000032:analysis*; D052617:Solid Phase Microextraction; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D007664:Ketosteroids