title: Evaluation of two commercially available chromogenic media for confirmation of methicillin-resistant Staphylococcus aureus from human, animal, and food samples.
abstract: We compared the diagnostic performance of two chromogenic media, Brilliance MRSA 2 agar (Thermo Fisher Scientific) and ChromID MRSA agar (bioMérieux), for MRSA confirmation of 239 Staphylococcus aureus isolates from clinical, animal and food samples. Statistically significant differences were not observed between MRSA confirmation by mecA/mecC PCR, and by culture in both chromogenic media. However, a statistically significant difference was observed between the results obtained by both chromogenic media (p = 0.003). Segregated analysis of the results depending on the origin of the isolates (clinical, animal, and food) revealed a significant lower performance in the MRSA confirmation of food-derived isolates by using Brilliance MRSA 2 agar in comparison to PCR confirmation (p = 0.003) or ChromID MRSA agar (p<0.001). Both chromogenic media provided a good diagnostic performance for detection of MRSA isolates of human and animal origin. In conclusion, the use of chromogenic agar plates for MRSA confirmation of S. aureus isolates can provide a good diagnostic performance (sensitivity >92% and specificity >89%) regardless of the type of chromogenic media used or the origin of the S. aureus isolates. However, our results revealed a lower diagnostic performance for MRSA confirmation of S. aureus isolates from food samples by using Brilliance MRSA 2 agar.
authors.affiliation: Instituto Tecnológico Agrario de Castilla y León, Valladolid, Spain.
authors.forename: Jaime
authors.initials: J
authors.lastname: Ariza-Miguel
authors.affiliation: Faculty of Food Science and Engineering, Dunarea de Jos University of Galati, Romania.
authors.forename: Elena-Alexandra
authors.initials: EA
authors.lastname: Oniciuc
authors.affiliation: Valladolid National Influenza Center, Valladolid, Spain.
authors.forename: Iván
authors.initials: I
authors.lastname: Sanz
authors.affiliation: Complejo Asistencial Universitario de León, León, Spain; Institute of Biomedicine (IBIOMED), University of León, Leon, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Fernández-Natal
authors.affiliation: Instituto Tecnológico Agrario de Castilla y León, Valladolid, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Hernández
authors.affiliation: Instituto Tecnológico Agrario de Castilla y León, Valladolid, Spain; Microbiology Section, Department of Biotechnology and Food science, Faculty of Science, University of Burgos, Burgos, Spain. Electronic address: rodlazda@gmail.com.
authors.forename: David
authors.initials: D
authors.lastname: Rodríguez-Lázaro
mesh_terms: D000362:Agar; D000818:Animals; D002863:Chromogenic Compounds / Q000737:chemistry*; D003470:Culture Media / Q000737:chemistry / Q000592:standards*; D005516:Food Microbiology / Q000379:methods* / Q000592:standards; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000302:isolation & purification*; D008828:Microbiological Techniques / Q000379:methods* / Q000592:standards; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000382:microbiology
chemical_list: D002863:Chromogenic Compounds; D003470:Culture Media; D000362:Agar
keywords: Chromogenic media; Methicillin-resistant Staphylococcus aureus; Screening