title: Protective effects of thymoquinone on experimental testicular ischaemia-reperfusion injury: an apoptotic, proliferative and biochemical study.
abstract: The objective of this study was to examine the effects of thymoquinone (TQ), which has antioxidant properties in the experimental testicular I/R model in rats in terms of its anti-apoptotic, proliferative and biochemical attributes. In our study, 24 male rats were divided into three groups: control group, I/R group and I/R+TQ group. Testicular torsion was created by rotating the left testis 720° in a clockwise direction. The ischaemia period was 4 h, and an orchiectomy was performed after 4 h of detorsion. Spermatogenesis and the mean seminiferous tubule diameter were significantly decreased in the I/R groups compared to the control group. Furthermore, TQ-treated animals displayed an improved histological appearance in the I/R group. It was also observed that treatment with TQ increased the activity of PCNA, which decreased as a result of I/R, and this treatment also reduced the number of TUNEL-positive cells. The I/R+TQ group showed a decrease in malondialdehyde levels and an increase in the activities of superoxide dismutase, catalase and glutathione peroxidase in comparison with the I/R group. It could be concluded that cytoprotective effects of TQ on the I/R testicles are via reduction of apoptosis, oxidative stress and lipid peroxidation.
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, University of Namik Kemal, Tekirdag, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Erboga
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, University of Namik Kemal, Tekirdag, Turkey.
authors.forename: C
authors.initials: C
authors.lastname: Aktas
authors.affiliation: Department of Urology, Faculty of Medicine, University of Namik Kemal, Tekirdag, Turkey.
authors.forename: O
authors.initials: O
authors.lastname: Kurt
authors.affiliation: Department of Anatomy, Faculty of Medicine, University of Namik Kemal, Tekirdag, Turkey.
authors.forename: R
authors.initials: R
authors.lastname: Uygur
authors.affiliation: Department of Anatomy, Faculty of Medicine, University of Namik Kemal, Tekirdag, Turkey.
authors.forename: V
authors.initials: V
authors.lastname: Caglar
authors.affiliation: Department of Anesthesiology and Reanimation, Faculty of Medicine, University of Namik Kemal, Tekirdag, Turkey.
authors.forename: B C
authors.initials: BC
authors.lastname: Turan
authors.affiliation: Department of Biostatistics, Faculty of Medicine, University of Namik Kemal, Tekirdag, Turkey.
authors.forename: B
authors.initials: B
authors.lastname: Topcu
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, University of Namik Kemal, Tekirdag, Turkey.
authors.forename: Z
authors.initials: Z
authors.lastname: Fidanol Erboga
authors.affiliation: Department of Biochemistry, Faculty of Medicine, University of Namik Kemal, Tekirdag, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Gurel
authors.affiliation: Department of Anatomy, Faculty of Medicine, University of Namik Kemal, Tekirdag, Turkey.
authors.forename: O A
authors.initials: OA
authors.lastname: Ozen
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D016227:Benzoquinones / Q000494:pharmacology*; D002374:Catalase / Q000187:drug effects / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects*; D005979:Glutathione Peroxidase / Q000187:drug effects / Q000378:metabolism; D020287:In Situ Nick-End Labeling; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009919:Orchiectomy; D009929:Organ Size; D018809:Proliferating Cell Nuclear Antigen / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology; D012671:Seminiferous Tubules / Q000187:drug effects / Q000473:pathology; D013091:Spermatogenesis / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013482:Superoxide Dismutase / Q000187:drug effects / Q000378:metabolism; D013737:Testis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology
chemical_list: D000975:Antioxidants; D016227:Benzoquinones; D018809:Proliferating Cell Nuclear Antigen; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; C003466:thymoquinone
keywords: Apoptosis; ischaemia and reperfusion; oxidative stress; testis; thymoquinone