title: Phytosiderophores revisited: 2'-deoxymugineic acid-mediated iron uptake triggers nitrogen assimilation in rice (Oryza sativa L.) seedlings.
abstract: Poaceae plants release phytosiderophores into the rhizosphere in order to chelate iron (Fe), which often exists in insoluble forms especially under high pH conditions. The impact of phytosiderophore treatment at the physiological and molecular levels in vivo remains largely elusive, although the biosynthesis of phytosiderophores and the transport of phytosiderophore-metal complexes have been well studied. We recently showed that the application of 30 μM of the chemically synthesized phytosiderophore 2'-deoxymugineic acid (DMA) was sufficient for apparent full recovery of otherwise considerably reduced growth of hydroponic rice seedlings at high pH. Moreover, unexpected induction of high-affinity nitrate transporter gene expression as well as nitrate reductase activity indicates that the nitrate response is linked to Fe homeostasis. These data shed light on the biological relevance of DMA not simply as a Fe chelator, but also as a trigger that promotes plant growth by reinforcing nitrate assimilation.
authors.affiliation: a Department of Botany ; Graduate School of Science; Kyoto University ; Kyoto , Japan.
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Araki
authors.forename: Kosuke
authors.initials: K
authors.lastname: Namba
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Murata
authors.forename: Jun
authors.initials: J
authors.lastname: Murata
mesh_terms: D001383:Azetidinecarboxylic Acid / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D007501:Iron / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism*; D012275:Oryza / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D036226:Seedlings / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D017262:Siderophores / Q000378:metabolism*
chemical_list: D017262:Siderophores; D001383:Azetidinecarboxylic Acid; C052864:2'-deoxymugineic acid; D007501:Iron; D009584:Nitrogen
keywords: 2′-deoxymugineic acid; DMA; DMA, 2′-deoxymugineic acid; Fe, iron; NA, nicotianamine; NR, nitrate reductase; NRT2, high-affinity nitrate transporter; Oryza sativa L.; SPAD, soil-plant analysis development; YSL, yellow stripe-like.; alkali tolerance; iron; nitrate assimilation; nitrate transport; plant growth