title: Leptin receptor polymorphism Gln223Arg (rs1137101) in oral squamous cell carcinoma and potentially malignant oral lesions.
abstract: The purpose of this study was to assess the LEPR gene Gln223Arg polymorphism (rs1137101) in oral squamous cell carcinoma (OSCC) and in potentially malignant oral lesions (PMOL) in comparison to normal oral mucosa in a Brazilian population. Smokers (n = 89) were selected from a representative sample of 471 individuals from the general population of Montes Claros, Brazil. Participants were age and gender matched to patients with OSCC (n = 25) and oral epithelial dysplasia (n = 25). We investigated the LEPR Gln223Arg polymorphism (A>G; rs1137101) in these groups. Genotype variants were assessed by RFLP-PCR, using MspI (HPAII) restriction endonuclease. The institutional review board of the Universidade Estadual de Montes Claros approved the study (process number 2667/2011). Written informed consent for this study was obtained from all participants. The GG genotype (Arg223Arg) appears to be the more relevant polymorphic variant in OSCC. It occurred, approximately, twice as frequently in OSCC patients than in the general population. In contrast, the A allele in its homozygosis form (Gln223Gln) is significantly associated with the development of PMOL; 80% of the samples from the PMOL group exhibit AA genotype. Our findings suggest new insights regarding LEPR gene variations in the development of OSCC and PMOL.
authors.affiliation: Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais Brazil.
authors.forename: Patrícia Luciana Batista
authors.initials: PL
authors.lastname: Domingos
authors.affiliation: Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais Brazil.
authors.forename: Lucyana Conceição
authors.initials: LC
authors.lastname: Farias
authors.affiliation: Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais Brazil.
authors.forename: Camila Santos
authors.initials: CS
authors.lastname: Pereira
authors.affiliation: School of Nursing, Universidade Federal de Minas Gerais, Belo Horizonte, MG Brazil.
authors.forename: Geórgia
authors.initials: G
authors.lastname: das Graças Pena
authors.affiliation: Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais Brazil.
authors.forename: Tatiana Carvalho
authors.initials: TC
authors.lastname: Reis
authors.affiliation: Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais Brazil.
authors.forename: Rosângela Ramos Veloso
authors.initials: RR
authors.lastname: Silva
authors.affiliation: Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais Brazil.
authors.forename: Carlos Alberto de Carvalho
authors.initials: CA
authors.lastname: Fraga
authors.affiliation: Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais Brazil.
authors.forename: Marcela Gonçalves
authors.initials: MG
authors.lastname: de Souza
authors.affiliation: Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais Brazil.
authors.forename: Mariana Batista
authors.initials: MB
authors.lastname: Soares
authors.affiliation: Dean of Research, Associação Educativa do Brasil (SOEBRAS), Montes Claros, Minas Gerais Brazil.
authors.forename: Kimberly Marie
authors.initials: KM
authors.lastname: Jones
authors.affiliation: Department of Biology, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais Brazil.
authors.forename: Elytania Veiga
authors.initials: EV
authors.lastname: Menezes
authors.affiliation: Department of Biology, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais Brazil.
authors.forename: Sérgio Avelino Mota
authors.initials: SA
authors.lastname: Nobre
authors.affiliation: Department of Medicine, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais Brazil.
authors.forename: João Felício
authors.initials: JF
authors.lastname: Rodrigues Neto
authors.affiliation: Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais Brazil.
authors.forename: Alfredo Maurício Batista
authors.initials: AM
authors.lastname: de Paula
authors.affiliation: School of Nursing, Universidade Federal de Minas Gerais, Belo Horizonte, MG Brazil.
authors.forename: Jorge Gustavo
authors.initials: JG
authors.lastname: Velásquez-Meléndez
authors.affiliation: Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais Brazil.
authors.forename: André Luiz
authors.initials: AL
authors.lastname: Sena Guimarães
keywords: Leptin; Leptin receptor; Oral squamous cell carcinoma; Polymorphism; Potentially malignant oral lesion; SNP