title: Angiotensin II type 1 receptor-associated protein plays a role in regulating the local renin-angiotensin system in HSC-T6 cells.
abstract: The aim of the current study was to examine the expression of the angiotensin II type 1 receptor‑associated protein (ATRAP) in the rat hepatic stellate cell line HSC‑T6 and to determine its interactions with the local renin‑angiotensin system (RAS). To achieve this goal, the effect of stimulating HSC‑T6 cells with angiotensin II (AngII) and angiotensin‑(1‑7) [Ang‑(1‑7)], on the expression of ATRAP, the angiotensin II type I receptor (AT1R), the Mas receptor and the angiotensin converting enzyme 2 (ACE2) 2, 6, 12, 18, 24 and 36 h after stimulation was investigated. Changes in expression were quantified at the gene and protein level using RT‑qPCR and western blotting, respectively. A single dose of AngII (1 µmol/l) significantly increased the gene expression of ATRAP at 12 h, whereas ACE2 gene expression levels were significantly increased at 6 h and then returned to baseline at 12 h, prior to becoming significantly lower. A single dose of Ang‑(1‑7) at the same concentration as AngII induced ATRAP gene expression, which became statistically significant at the 6 h time‑point, reached a peak at 12 h and remained elevated throughout the experimental time‑course. In addition, ACE2 mRNA expression was significantly suppressed by Ang‑(1‑7) at 6 h, reaching its lowest expression level at 24 h. The expression of AT1R and the Mas receptor were unaffected by stimulation with AngII and Ang‑(1‑7). The western blotting results were generally consistent with the mRNA expression data. In conclusion, it was identified that ATRAP is endogenously expressed in HSC‑T6 cells and therefore, may be critical in regulating the local RAS in these cells.
authors.affiliation: Department of Hepatobiliary Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
authors.forename: Haiyang
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Hepatobiliary Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Hepatobiliary Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
authors.forename: Zhonglin
authors.initials: Z
authors.lastname: Cui
authors.affiliation: Department of Hepatobiliary Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
authors.forename: Biao
authors.initials: B
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat‑sen University, Guangzhou, Guangdong 510060, P.R. China.
authors.forename: Yunwei
authors.initials: Y
authors.lastname: Hu
mesh_terms: D000803:Angiotensin I / Q000378:metabolism*; D000804:Angiotensin II / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D005786:Gene Expression Regulation; D010446:Peptide Fragments / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011945:Receptors, Angiotensin / Q000235:genetics / Q000378:metabolism*; D012084:Renin-Angiotensin System
chemical_list: C524087:Agtrap protein, rat; D010446:Peptide Fragments; D012333:RNA, Messenger; D011945:Receptors, Angiotensin; D000804:Angiotensin II; D000803:Angiotensin I; C118790:angiotensin I (1-7)