title: [The value of blastocyst culture on preimplantation genetic diagnosis].
abstract: OBJECTIVE
  To estimate the value of blastocyst culture for preimplantation genetic diagnosis (PGD).
  
  
  METHODS
  Day 3 embryos were biopsied and analyzed with fluorescence in situ hybridization (FISH) technique. Embryos with normal FISH results were cultured into blastocysts, and the ones with better morphology scores were transferred. Fourteen embryos with abnormal FISH results were cultured into blastocysts. Part of the cells taken from the blastocysts were amplified by whole genomic amplification (WGA) and assessed by array-based comparative genomic hybridization (array-CGH) analysis.
  
  
  RESULTS
  Six blastocysts with normal FISH results were transferred in 5 cycles. Four healthy babies of 3 cycles were delivered. Another one was a singleton pregnancy but with embryo growth arrest, whose villus karyotype was normal. Fourteen embryos with abnormal FISH results were cultured into blastocysts and analyzed by array-CGH. Six blastocysts were normal by array-CGH.
  
  
  CONCLUSIONS
  FISH combined with blastocyst culture may further ensure the accuracy of PGD result. Detection at the blastocyst stage can avoid false positive results and mosaic interferences on Day 3 stage and are therefore more authentic.
authors.affiliation: Center of Reproduction and Genetics, Suzhou Municipal Hospital, Suzhou, Jiangsu 215002, P.R.China. hongliszivf@163.com; richardchoy@cuhk.edu.hk.
authors.forename: Jian
authors.initials: J
authors.lastname: Ou
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Yanlin
authors.initials: Y
authors.lastname: Ma
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhou
authors.forename: Jie
authors.initials: J
authors.lastname: Ding
authors.forename: Fuxin
authors.initials: F
authors.lastname: Wang
authors.forename: Chengying
authors.initials: C
authors.lastname: Duan
authors.forename: Linjiang
authors.initials: L
authors.lastname: Li
authors.forename: Aiyan
authors.initials: A
authors.lastname: Zheng
authors.forename: Wilson
authors.initials: W
authors.lastname: Chong
authors.forename: Richard
authors.initials: R
authors.lastname: Choy
authors.forename: Hong
authors.initials: H
authors.lastname: Li
mesh_terms: D000328:Adult; D001755:Blastocyst / Q000166:cytology*; D055028:Comparative Genomic Hybridization / Q000379:methods*; D004624:Embryo Transfer; D005260:Female; D030342:Genetic Diseases, Inborn / Q000175:diagnosis* / Q000196:embryology / Q000235:genetics / Q000517:prevention & control; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008297:Male; D011247:Pregnancy; D019836:Preimplantation Diagnosis / Q000379:methods*