title: A series of medium and high copy number arabinose-inducible Escherichia coli expression vectors compatible with pBR322 and pACYC184.
abstract: The original pBAD24 plasmid and the derived lower copy number (the pBAD322 series) expression vectors have been widely used in Escherichia coli, Salmonella enterica, and related bacteria. However, a flexible pBAD expression system has been available only in pMB1 (ColE1) vectors. We report a series of pBAD vectors that replicate using the origin of plasmid RSF1030 that are compatible with pMB1 (ColE1) and p15A (pACYC) vectors. Both high (≥pBAD24) and medium (~pBAD322) copy number plasmids encoding resistance to ampicillin, chloramphenicol, kanamycin, tetracycline, spectinomycin/streptomycin, gentamycin, or trimethoprim are available.
authors.affiliation: Department of Microbiology, University of Illinois, B103 Chemical and Life Sciences Laboratory, 601S. Goodwin Ave., Urbana, IL 61801, USA.
authors.forename: Vandana
authors.initials: V
authors.lastname: Chakravartty
authors.affiliation: Department of Microbiology, University of Illinois, B103 Chemical and Life Sciences Laboratory, 601S. Goodwin Ave., Urbana, IL 61801, USA; Department of Biochemistry, University of Illinois, 419 Roger Adams Laboratory, 600 South Mathews Ave., Urbana, IL 61801, USA. Electronic address: j-cronan@life.uiuc.edu.
authors.forename: John E
authors.initials: JE
authors.lastname: Cronan
mesh_terms: D001089:Arabinose / Q000494:pharmacology*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D018628:Gene Dosage; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D005822:Genetic Vectors / Q000235:genetics*; D042822:Genomic Instability; D010957:Plasmids / Q000235:genetics*
chemical_list: D001089:Arabinose
keywords: Copy number; Expression levels; Plasmid Compatibility; RSF1030; pBAD24; pBR322