title: Geno- and phenotyping of human neutrophil antigens.
abstract: For typing of human neutrophil antigens (HNA) usually genotyping techniques are used, except for HNA-2, which-due to a gene expression defect-requires phenotyping. For genotyping, several techniques have been described. Most reference laboratories use variations of the polymerase chain reaction (PCR) for antigen typing which showed good results in international quality assessment exercises. The granulocyte immunofluorescence test has been the gold standard technique for phenotyping for all HNA antigens except for HNA-3a and -3b phenotyping. The expression of the latter antigens on neutrophils is often better shown by the use of the granulocyte agglutination test.
authors.affiliation: German Red Cross Blood Service West, Hagen, Germany, a.reil@bsdwest.de.
authors.forename: Angelika
authors.initials: A
authors.lastname: Reil
authors.forename: Jürgen
authors.initials: J
authors.lastname: Bux
mesh_terms: D005455:Fluorescent Antibody Technique / Q000379:methods; D058851:GPI-Linked Proteins / Q000235:genetics; D060005:Genotyping Techniques / Q000379:methods*; D006098:Granulocytes / Q000378:metabolism; D006801:Humans; D007519:Isoantigens / Q000235:genetics*; D009504:Neutrophils / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D011956:Receptors, Cell Surface / Q000235:genetics*
chemical_list: C407242:CD177 protein, human; D058851:GPI-Linked Proteins; C518134:HNA-3a antigen, human; D007519:Isoantigens; D011956:Receptors, Cell Surface; C067704:neutrophil-specific antigen NA1, human