title: Microfluidic Superheating for Peptide Sequence Elucidation.
abstract: Herein, we introduce microfluidic superheating as a new method for peptide fragmentation prior to mass spectrometric analysis. The superheating conditions were found to be stable up to 240 °C for more than 30 min without elevated pressure or boiling of the aqueous sample. As proof of principle, we exposed the peptides ACTH1-10 and OVA257-264 to various superheating conditions, causing different degrees of decomposition. Optimized superheating conditions resulted in the entire peptide ladder sequence of the y-ions, allowing the amino acid sequence to be deduced from a single-stage mass spectrum. Thus, obtaining information in the same quality as from tandem mass spectrometry can be achieved by a single superheating step.
authors.affiliation: ∥KIST Europe, Microfluidics, 66123 Saarbrücken, Germany.|⊥Twente University, MESA+, Institute for Nanotechnology, 7500 AE Enschede, Netherlands.
authors.forename: Matthias O
authors.initials: MO
authors.lastname: Altmeyer
authors.affiliation: ∥KIST Europe, Microfluidics, 66123 Saarbrücken, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Manz
authors.affiliation: ∥KIST Europe, Microfluidics, 66123 Saarbrücken, Germany.|§Central European Institute of Technology, Brno University of Technology, CZ-616 00 Brno, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Neužil
mesh_terms: D006358:Hot Temperature; D013058:Mass Spectrometry / Q000295:instrumentation; D046210:Microfluidic Analytical Techniques; D010446:Peptide Fragments / Q000737:chemistry*; D020539:Sequence Analysis, Protein / Q000379:methods*
chemical_list: D010446:Peptide Fragments