title: [Selective-differential nutrient medium "Shewanella IRHLS agar" for isolation of Shewanella genus bacteria].
abstract: OBJECTIVE
  Development of a selective-differential nutrient medium for isolation of Shewanella genus bacteria.
  
  
  METHODS
  73 strains of Shewanella bacteria (S. algae--3, S. baltica--26, S. putrefaciens--44) and 80 strains of 22 other bacteria genera were used. Shewanella species were identified by methods and criteria proposed by Nozue H. et al., 1992; Khashe S. et al., 1998.
  
  
  RESULTS
  Nutrient media "Shewanella IRHLS Agar" for shewanella isolation was developed. Medium selective factors: irgazan DP-300 (I). 0.14-0.2 g/l and rifampicin (R) 0.0005-0.001 g/l. Shevanella colonies were detected by the production of hydrogen sulfide (H), lipase presence (L), lack of sorbitol fermentation (S). The medium suppressed the growth of hydrogen sulfide producers (Salmonella, Proteus) and blocked hydrogen sulfide production by Citrobacter. Growth of Escherichia, Enterobacter, Klebsiella, Shigella, Staphylococcus, Bacillus was also suppressed, Analytical sensitivity of the medium was 1-2 CFU/ml for Shewanella and Stenotrophomonas, Aerombnas, Serratia genera bacteria. 72 strains of Shewanella were isolated from water of Neva river in this medium, 91.7 ± 3.2% of those produced H2S. 1 strain of S. algae was isolated from clinical material.
  
  
  CONCLUSIONS
  The developed media allows to use it in a complex for Stenotrophomo- nas sp., Aeromonas sp., Serratia sp., Citrobactersp. and Shewanella bacteria isolation.
authors.forename: E P
authors.initials: EP
authors.lastname: Sivolodsky
mesh_terms: D000362:Agar / Q000737:chemistry; D000898:Antibiosis / Q000502:physiology*; D001426:Bacterial Proteins / Q000378:metabolism; D002954:Citrobacter; D015169:Colony Count, Microbial; D003470:Culture Media / Q000737:chemistry* / Q000494:pharmacology; D004755:Enterobacteriaceae; D006862:Hydrogen Sulfide / Q000378:metabolism*; D008049:Lipase / Q000378:metabolism; D011511:Proteus; D012475:Salmonella; D020592:Shewanella / Q000187:drug effects / Q000254:growth & development* / Q000302:isolation & purification; D013210:Staphylococcus
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D000362:Agar; D008049:Lipase; D006862:Hydrogen Sulfide