title: MiR-30a suppresses non-small cell lung cancer progression through AKT signaling pathway by targeting IGF1R.
abstract: MicroRNAs play critical roles in the development and progression of human cancers. Although miR&mdash;30a has been suggested to function as a tumor repressor in several tumors, its role in non&mdash;small cell lung cancer (NSCLC) has not been investigated in detail. This study investigated the expression and role of miR&mdash;30a in human NSCLC. The expression of miR&mdash;30a is significantly decreased in clinical NSCLC tissues and cell lines. Overexpression of miR&mdash;30a inhibited NSCLC cell proliferation, G1/S and S/G2 transition in vitro, whereas suppression of miR&mdash;30a facilitated NSCLC cell proliferation, G1/S and S/G2 transition. Using a luciferase reporter assay, insulin&mdash;like growth factor 1 receptor (IGF1R) was determined to be a direct target of miR&mdash;30a. Furthermore, silencing IGF1R resulted in the same biologic effects of miR&mdash;30a overexpression in NSCLC cells, which included suppressed NSCLC cell proliferation and trigering cell cycle arrest through PI3K/AKT signaling pathway by inhibiting cell cycle regulators (CDK2, CDK4, Cyclin A2 , Cyclin D1). These results demonstrate that miR&mdash;30a influences NSCLC progression through PI3K/AKT signaling pathway by targeting IGF1R in A549 cells, which suggest miR&mdash;30a as a novel strategy for NSCLC diagnosis and treatment.
authors.affiliation: The First Affiliated Hospital of Medical College, Xi&apos;an Jiaotong University Department of Thoracic Surgery Shaanxi Xi&apos;an China.
authors.forename: X P
authors.initials: XP
authors.lastname: Wen
authors.affiliation: The First Affiliated Hospital of Medical College, Xi&apos;an Jiaotong University Department of Radiation Oncology Shaanxi Xi&apos;an China.
authors.forename: H L
authors.initials: HL
authors.lastname: Ma
authors.affiliation: School of Basic Medical Sciences, Xi&apos;an Jiaotong University Health Science Center Department of Cell Biology and Genetics Shaanxi Xi&apos;an China.
authors.forename: L Y
authors.initials: LY
authors.lastname: Zhao
authors.affiliation: The First Affiliated Hospital of Medical College, Xi&apos;an Jiaotong University Department of geriatric&mdash;cardiovascular Shaanxi Xi&apos;an China.
authors.forename: W
authors.initials: W
authors.lastname: Zhang
authors.affiliation: The First Affiliated Hospital of Medical College, Xi&apos;an Jiaotong University Department of Oncology Surgery Shaanxi Xi&apos;an China dangchengxue2014&commat;126.com.
authors.forename: C X
authors.initials: CX
authors.lastname: Dang
mesh_terms: D017209:Apoptosis; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D018450:Disease Progression; D005260:Female; D059585:G1 Phase Cell Cycle Checkpoints / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D008875:Middle Aged; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D034622:RNA Interference; D034741:RNA, Small Interfering; D017526:Receptor, IGF Type 1; D017451:Receptors, Somatomedin / Q000235:genetics*; D059807:S Phase Cell Cycle Checkpoints / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics
chemical_list: D035683:MicroRNAs; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D034741:RNA, Small Interfering; D017526:Receptor, IGF Type 1; D017451:Receptors, Somatomedin; C000597236:IGF1R protein, human; C546086:MIRN30b microRNA, human