title: The two-step assemblies of basic-amino-Acid-rich Peptide with a highly charged polyoxometalate.
abstract: Two-step assembly of a peptide from HPV16 L1 with a highly charged europium-substituted polyoxometalate (POM) cluster, accompanying a great luminescence enhancement of the inorganic polyanions, is reported. The mechanism is discussed in detail by analyzing the thermodynamic parameters from isothermal titration calorimetry (ITC), time-resolved fluorescent and NMR spectra. By comparing the actions of the peptide analogues, a binding process and model are proposed accordingly. The driving forces in each binding step are clarified, and the initial POM aggregation, basic-sequence and hydrophobic C termini of peptide are revealed to contribute essentially to the two-step assembly. The present study demonstrates both a meaningful preparation for bioinorganic materials and a strategy using POMs to modulate the assembly of peptides and even proteins, which could be extended to other proteins and/or viruses by using peptides and POMs with similar properties.
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, Jilin University, No. 2699, Qianjin Street, Changchun, 130012 (China).
authors.forename: Teng
authors.initials: T
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, Jilin University, No. 2699, Qianjin Street, Changchun, 130012 (China).
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Li
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, Jilin University, No. 2699, Qianjin Street, Changchun, 130012 (China). yqwu@jlu.edu.cn.
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, Jilin University, No. 2699, Qianjin Street, Changchun, 130012 (China).
authors.forename: Yizhan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, Jilin University, No. 2699, Qianjin Street, Changchun, 130012 (China). wulx@jlu.edu.cn.
authors.forename: Lixin
authors.initials: L
authors.lastname: Wu
mesh_terms: D024361:Amino Acids, Basic / Q000737:chemistry*; D005063:Europium / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D049408:Luminescent Agents / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D010455:Peptides / Q000737:chemistry*; D011485:Protein Binding; D013816:Thermodynamics; D017973:Tungsten Compounds / Q000737:chemistry*; D000099294:Polyoxometalates
chemical_list: D024361:Amino Acids, Basic; D005063:Europium; D049408:Luminescent Agents; D010455:Peptides; D017973:Tungsten Compounds; C102296:polyoxometalate I; D000099294:Polyoxometalates
keywords: binding mechanisms; luminescence enhancement; peptides; polyoxometalates; self-assembly