title: Protein clustering and spatial organization in T-cells.
abstract: T-cell protein microclusters have until recently been investigable only as microscale entities with their composition and structure being discerned by biochemistry or diffraction-limited light microscopy. With the advent of super resolution microscopy comes the ability to interrogate the structure and function of these clusters at the single molecule level by producing highly accurate pointillist maps of single molecule locations at ~20nm resolution. Analysis tools have also been developed to provide rich descriptors of the pointillist data, allowing us to pose questions about the nanoscale organization which governs the local and cell wide responses required of a migratory T-cell.
authors.affiliation: *Department of Physics and Randall Division of Cell and Molecular Biophysics, King's College London, London SE1 1UL, U.K.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Shannon
authors.affiliation: *Department of Physics and Randall Division of Cell and Molecular Biophysics, King's College London, London SE1 1UL, U.K.
authors.forename: Garth
authors.initials: G
authors.lastname: Burn
authors.affiliation: †Academic Department of Rheumatology, Division of Immunology, Infection and Inflammatory Disease, Faculty of Life Sciences and Medicine, King's College London, London SE1 1UL, U.K.
authors.forename: Andrew
authors.initials: A
authors.lastname: Cope
authors.affiliation: †Academic Department of Rheumatology, Division of Immunology, Infection and Inflammatory Disease, Faculty of Life Sciences and Medicine, King's College London, London SE1 1UL, U.K.
authors.forename: Georgina
authors.initials: G
authors.lastname: Cornish
authors.affiliation: *Department of Physics and Randall Division of Cell and Molecular Biophysics, King's College London, London SE1 1UL, U.K.
authors.forename: Dylan M
authors.initials: DM
authors.lastname: Owen
mesh_terms: D002465:Cell Movement / Q000276:immunology; D054992:Immunological Synapses / Q000737:chemistry* / Q000648:ultrastructure; D016023:Integrins / Q000737:chemistry; D008856:Microscopy, Fluorescence; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry* / Q000648:ultrastructure; D013601:T-Lymphocytes / Q000737:chemistry* / Q000276:immunology / Q000648:ultrastructure
chemical_list: D016023:Integrins; D011506:Proteins