title: [The noninvasive evaluation of degree of expression of fibrosis of liver and significance of polymorphism of gene of hyaluronic acid under chronic hepatitis C].
abstract: The study was carries out to evaluate degree of expression of fibrosis, reparation processes in liver and value of polymorphism of gene of hyaluronic acid HASI (rs11084111) in progression of affection of liver in patients with chronic hepatitis C. The sampling included 100 patients with chronic hepatitis C. The control group included 83 healthy donors. The blood serum was tested to detect concentration of hyaluronic acid and alpha-fetoprotein. The stage of liver fibrosis (F) was evaluated by using ultrasound fibroflexography The polymorphism of gene (rs11084111) was analysed by polymerase chain reaction technique. In the group of patients with F1 the average concentration of hyaluronic acid in blood serum in 1.8 times surpassed this indicator in group with F0. The concentration of hyaluronic acid was almost 2 times higher under F3 as compared with F1-F2. This indicator permitted differentiating F3 and F4 which followed by activation of cytolysis and cholestasis in F1 and F3 and by increasing of level of alpha-fetoprotein at stages F1 and F4. The study detected no statistically significant difference between rates of genotypes and alleles of gene HASI (rs11084111) in groups of healthy patients and patients with chronic hepatitis C. The direct relationships are established between hyaluronic acid and markers of cytolysis, cholestasis, alpha-fetoprotein (p = 0.001), viral load (p = 0.003) liver elasticity index according fibroflexography data (p < 0.001) and fibrosis index (p < 0.001). The established relationships indicate association of hepatofibrosis with cytolysis, cholestasis, hepatocytes regeneration and virus activity. The hyaluronic acid permits to stratify minimal expressed fibrosis and also the transition of disease to the stage of cirrhosis.
authors.forename: I A
authors.initials: IA
authors.lastname: Bulatova
authors.forename: A P
authors.initials: AP
authors.lastname: Schekotova
authors.forename: A V
authors.initials: AV
authors.lastname: Krivtsov
authors.forename: V V
authors.initials: VV
authors.lastname: Schekotov
authors.forename: A I
authors.initials: AI
authors.lastname: Pavlov
mesh_terms: D000328:Adult; D005260:Female; D014453:Glucuronosyltransferase / Q000235:genetics*; D019698:Hepatitis C, Chronic / Q000097:blood* / Q000150:complications / Q000473:pathology / Q000821:virology; D006801:Humans; D000076002:Hyaluronan Synthases; D006820:Hyaluronic Acid / Q000097:blood*; D008099:Liver / Q000378:metabolism / Q000821:virology; D008103:Liver Cirrhosis / Q000097:blood* / Q000150:complications / Q000235:genetics / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D010976:Platelet Count; D011110:Polymorphism, Genetic; D011379:Prognosis; D000509:alpha-Fetoproteins / Q000378:metabolism
chemical_list: D000509:alpha-Fetoproteins; D006820:Hyaluronic Acid; D014453:Glucuronosyltransferase; C523794:HAS1 protein, human; D000076002:Hyaluronan Synthases