title: NF-κB pathway controls mitochondrial dynamics.
abstract: The Optic atrophy 1 protein (OPA1) is a key element in the dynamics and morphology of mitochondria. We demonstrated that the absence of IκB kinase-α, which is a key element of the nonclassical NF-κB pathway, has an impact on the mitochondrial network morphology and OPA1 expression. In contrast, the absence of NF-κB essential modulator (NEMO) or IκB kinase-β, both of which are essential for the canonical NF-κB pathway, has no impact on mitochondrial dynamics. Whereas Parkin has been reported to positively regulate the expression of OPA1 through NEMO, herein we found that PARK2 overexpression did not modify the expression of OPA1. PARK2 expression reduced the levels of Bax, and it prevented stress-induced cell death only in Bak-deficient mouse embryonic fibroblast cells. Collectively, our results point out a role of the nonclassical NF-κB pathway in the regulation of mitochondrial dynamics and OPA1 expression.
authors.affiliation: CNRS FR 3636, Université Paris Descartes, Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Laforge
authors.affiliation: CNRS FR 3636, Université Paris Descartes, Paris, France.|Parasite Disease Group, Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal.
authors.forename: V
authors.initials: V
authors.lastname: Rodrigues
authors.affiliation: Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho; ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, Portugal.
authors.forename: R
authors.initials: R
authors.lastname: Silvestre
authors.affiliation: Inserm, U 1127, F-75013 Paris, France.|CNRS, UMR 7225, F-75013 Paris, France.|Sorbonne Universités, UPMC Univ Paris 06, UMR S 1127, F-75013 Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Gautier
authors.affiliation: Unité de Signalisation Moléculaire et Activation Cellulaire, Institut Pasteur, Unité CNRS URA 2582, Paris, France.
authors.forename: R
authors.initials: R
authors.lastname: Weil
authors.affiliation: Inserm, U 1127, F-75013 Paris, France.|CNRS, UMR 7225, F-75013 Paris, France.|Sorbonne Universités, UPMC Univ Paris 06, UMR S 1127, F-75013 Paris, France.
authors.forename: O
authors.initials: O
authors.lastname: Corti
authors.affiliation: CNRS FR 3636, Université Paris Descartes, Paris, France.|Centre de Recherche en Infectiologie, Université Laval, Québec, Canada.
authors.forename: J
authors.initials: J
authors.lastname: Estaquier
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D002460:Cell Line; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology; D020558:GTP Phosphohydrolases / Q000096:biosynthesis* / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D051550:I-kappa B Kinase / Q000096:biosynthesis / Q000235:genetics; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008928:Mitochondria / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D016328:NF-kappa B / Q000235:genetics; D015398:Signal Transduction; D044767:Ubiquitin-Protein Ligases / Q000235:genetics* / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000235:genetics
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; C514981:NEMO protein, mouse; D016328:NF-kappa B; D051028:bcl-2-Associated X Protein; D044767:Ubiquitin-Protein Ligases; C111567:parkin protein; D051550:I-kappa B Kinase; D020558:GTP Phosphohydrolases; C498809:Opa1 protein, mouse