title: Establishment and cryopreservation of a giant panda skeletal muscle-derived cell line.
abstract: The giant panda Ailuropoda melanoleuca is an endangered species and is a symbol for wildlife conservation. Although efforts have been made to protect this rare and endangered species through breeding and conservative biology, the long-term preservation of giant panda genome resources (gametes, tissues, organs, genomic libraries, etc.) is still a practical option. In this study, the giant panda skeletal muscle-derived cell line was successfully established via primary explants culture and cryopreservation techniques. The population doubling time of giant panda skeletal cells was approximately 33.8 h, and this population maintained a high cell viability before and after cryopreservation (95.6% and 90.7%, respectively). The two skeletal muscle-specific genes SMYD1 and MYF6 were expressed and detected by RT-PCR in the giant panda skeletal muscle-derived cell line. Karyotyping analysis revealed that the frequencies of giant panda skeletal muscle cells showing a chromosome number of 2n=42 ranged from 90.6∼94.2%. Thus, the giant panda skeletal muscle-derived cell line provides a vital resource and material platform for further studies and is likely to be useful for the protection of this rare and endangered species.
authors.affiliation: 1College of Life Sciences, Zhejiang University, Hangzhou, China.
authors.forename: Fang-Jian
authors.initials: FJ
authors.lastname: Yu
authors.affiliation: 2Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Ya'an, Sichuan Province, China.|5Key Laboratory for Natural Resources Protection and Sustainable Utilization of Sichuan Province, Chengdu, China.
authors.forename: Chang-Jun
authors.initials: CJ
authors.lastname: Zeng
authors.affiliation: 2Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Ya'an, Sichuan Province, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: 3China Conservation and Research Center for the Giant Panda, Wolong, China.
authors.forename: Cheng-Dong
authors.initials: CD
authors.lastname: Wang
authors.affiliation: 4Sichuan Province Natural Resources Science Academy, Chengdu, China.|5Key Laboratory for Natural Resources Protection and Sustainable Utilization of Sichuan Province, Chengdu, China.
authors.forename: Tie-Yi
authors.initials: TY
authors.lastname: Xiong
authors.affiliation: 1College of Life Sciences, Zhejiang University, Hangzhou, China.
authors.forename: Sheng-Guo
authors.initials: SG
authors.lastname: Fang
authors.affiliation: 3China Conservation and Research Center for the Giant Panda, Wolong, China.|5Key Laboratory for Natural Resources Protection and Sustainable Utilization of Sichuan Province, Chengdu, China.
authors.forename: He-Min
authors.initials: HM
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D033481:Chromosomes, Mammalian / Q000378:metabolism; D015925:Cryopreservation / Q000379:methods*; D005786:Gene Expression Regulation; D007621:Karyotyping; D008297:Male; D008677:Metaphase; D032342:Muscle Cells / Q000166:cytology; D018482:Muscle, Skeletal / Q000166:cytology*; D009928:Organ Specificity / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D001503:Ursidae
chemical_list: D012333:RNA, Messenger