title: Panton-Valentine leukocidin in community and hospital-acquiredStaphylococcus aureusstrains.
abstract: Staphylococcus aureuscauses serious hospital-acquired (HA) and community-acquired (CA) infections. Skin and soft-tissue infections especially are sometimes caused by strains harbouring Panton-Valentine leukocidin (PVL). PVL belongs to a family of bi-component leukocidal toxins produced by staphylococci. It is a pore-forming toxin encoded bylukF-PVandlukS-PV. A total of 70S. aureusstrains: 38 (54%) methicillin-resistant (MRSA) and 32 (46%) methicillin-susceptible (MSSA), were isolated from patients admitted to Dicle University Hospital (Turkey). Identification ofS. aureusand antibiotics-susceptibility testing were performed with PHOENIX 100. PVL genes andmecAgenes were detected by polymerase chain reaction. Of the 70 studied strains, 36 ones (51%) were community acquired and 34 ones (49%) were hospital acquired . A total of 38 (54%) strains were positive formecA(mecA+), of which 32 ones (84%) were HA. Of themecA-strains, 30 (94%) were CA. Of the 70 studied strains, 12 (17%) strains were PVL+: 8 (22%) of the 36 CA strains and 4 (12%) of the 34 HA strains. Of the 12 PVL+strains, 4 strains weremecA+. The PVL positivity rate was 25% in MSSA, whereas 10.5% in MRSA. Of the overall PVL+strains, seven strains were obtained from wounds; four ones from skin abscess; and one from blood culture. Taken together, the obtained results showed a substantial level of PVL genes in the studied region. Although PVL is known as a common virulence factor of CA MRSA, HA MRSA isolates in our study showed a considerable rate of PVL positivity.
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, Dicle University , Diyarbakır , Turkey.
authors.forename: Tuncer
authors.initials: T
authors.lastname: Özekinci
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, Yıldırım Beyazıt University , Ankara , Turkey.
authors.forename: Tuba
authors.initials: T
authors.lastname: Dal
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, Ondokuz Mayıs University , Samsun , Turkey.
authors.forename: Keramettin
authors.initials: K
authors.lastname: Yanık
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, Dicle University , Diyarbakır , Turkey.
authors.forename: Nida
authors.initials: N
authors.lastname: Özcan
authors.affiliation: Department of Medical Microbiology, Ergani State Hospital , Diyarbakır , Turkey.
authors.forename: Şükran
authors.initials: Ş
authors.lastname: Can
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, Dicle University , Diyarbakır , Turkey.
authors.forename: Alicem
authors.initials: A
authors.lastname: Tekin
authors.affiliation: Department of Genetics, Faculty of Veterinary Medicine, Dicle University , Diyarbakır , Turkey.
authors.forename: Halil İbrahim
authors.initials: Hİ
authors.lastname: Yıldırım
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, Dicle University , Diyarbakır , Turkey.
authors.forename: İdris
authors.initials: İ
authors.lastname: Kandemir
keywords: MRSA; MSSA; Panton-Valentine leukocidin; Staphylococcus aureus