title: Hydrogel nanoparticle encapsulated plasmid as a suitable gene delivery system.
abstract: To facilitate the delivery of genetic material, the use of appropriate carriers such as polymers is necessary. Nanoparticles comprising of chitosan-alginate polymers were formed through pregel preparation method. Chi/Alg nanoparticles had a mean Z-Average diameter of 161.8 nm and mean zeta 29.3 mV, respectively. The ability of plasmidcomplex in preventing DNA migration showed Chi/Alg nanoparticles have great capacity to maintain plasmid. The efficiency of nanoparticles for transfection of pEGFP-N1 plasmid in the cultured HEK 293 cells was measured by flow cytometry. Cell viability assays indicated that nanoparticles had no toxic effect on HEK 293 cells after 4 or 24 h. Our suitable candidate for gene delivery would be Alg/Chi nanoparticles.
authors.forename: A
authors.initials: A
authors.lastname: Rafieei
authors.forename: F
authors.initials: F
authors.lastname: Riazi-Rad
authors.forename: M H
authors.initials: MH
authors.lastname: Alimohammadian
authors.forename: T
authors.initials: T
authors.lastname: Gazori
authors.forename: S M R
authors.initials: SM
authors.lastname: Fatemi
authors.forename: M
authors.initials: M
authors.lastname: Havaskary
mesh_terms: D000464:Alginates / Q000737:chemistry*; D002470:Cell Survival; D048271:Chitosan / Q000737:chemistry*; D004339:Drug Compounding; D005434:Flow Cytometry; D015870:Gene Expression; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D020723:Glucuronic Acid / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D010957:Plasmids / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020100:Hydrogels; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D020723:Glucuronic Acid; D048271:Chitosan