title: Intermittent administration of parathyroid hormone improves the repairing process of rat calvaria defects: A histomorphometric and radiodensitometric study.
abstract: BACKGROUND
  The aim of this study was to evaluate the effects of intermittent treatment of parathyroid hormone (PTH (1-34)) on the bone regeneration of critically-sized rat calvarial bone defects.
  
  
  METHODS
  Thirty-two male rats were trephined (4mm full-thickness diameter), in the central part of the parietal bones and divided into 2 groups of 16. The PTH group received subcutaneous injections of PTH (1-34) at 40µg/kg, 3 times a week and the control (CTL) group received the vehicle in the same regimen. The rats were sacrificed at 4 weeks post-treatment regimen, the parietal bones were extracted and samples were evaluated through histomorphometry and radiodensitometry.
  
  
  RESULTS
  The histological observations showed that the PTH group presented more "island-like" new bone between the defect margins with fibrous tissues than did the CTL group. The PTH group significantly exhibited greater histologic bone formation than did the CTL group (1.5mm ±0.7; 1.9 mm ± 0.6, p<0.05/ for residual bone defect). The radiodensitometry analysis revealed significant differences among the PTH and CTL groups (2.1 Al eq. ±0.04; 1.8Al eq. ±0.06, p<0.05), demonstrating an increase in bone mineral density. The PTH treatment contributed to the bone formation with a higher amount of mineral and/or fibrous tissue when compared with the CTL group.
  
  
  CONCLUSIONS
  The results suggest that it was possible to increase the process of bone regeneration by accelerating the healing process in rat calvarial defects through intermittent administration of the PTH treatment.
authors.affiliation: Universidade Federal do Piauí - UFPI, Campus Ministro Reis Veloso, Colegiado de Biomedicina, Av. São Sebastião, 2819, Reis Veloso, Parnaíba -PI -Brazil, 64204-035, vasconcelos@ufpi.edu.br.
authors.forename: Eduardo-de-Paula
authors.initials: ED
authors.lastname: Silva
authors.forename: Daniel-Fernando-Pereira
authors.initials: DF
authors.lastname: Vasconcelos
authors.forename: Marcelo-Rocha
authors.initials: MR
authors.lastname: Marques
authors.forename: Marco-Antônio Dias da
authors.initials: MA
authors.lastname: Silva
authors.forename: Flávio-Ricardo
authors.initials: FR
authors.lastname: Manzi
authors.forename: Silvana-Pereira
authors.initials: SP
authors.lastname: Barros
mesh_terms: D000818:Animals; D015519:Bone Density; D001861:Bone Regeneration / Q000187:drug effects*; D008297:Male; D010281:Parathyroid Hormone / Q000008:administration & dosage*; D011859:Radiography; D051381:Rats; D017208:Rats, Wistar; D012886:Skull / Q000033:anatomy & histology / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000502:physiology*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D010281:Parathyroid Hormone