title: Overexpression of Arabidopsis Dehydration-Responsive Element-Binding protein 2A confers tolerance to salinity stress to transgenic canola.
abstract: Stress responsive transcriptional regulation is an adaptive strategy of plants that alleviates the adverse effects of environmental stresses. The ectopic overexpression of Dehydration-Responsive Element Binding transcription factors (DREBs) either in homologous or in heterologous plants are the classical transcriptional regulators involved in plant responses to drought, salt and cold stresses. To elucidate the transcriptional mechanism associated with the DREB2A gene after removing PEST sequence, which acts as a signal peptide for protein degradation, 34 transgenic T0 canola plants overexpressing DREB2A were developed. The quantitative Real time PCR of transgenic plants showed higher expression of downstream stress-responsive genes including COR14, HSF3, HSP70, PEROX and RD20. The transgenic plants exhibited enhanced tolerance to salt stress. At the high concentration of NaCl the growth of non-transformed plants had been clearly diminished, whereas transgenic line was survived. These results indicated that transformed DREB2A gene might improve the plant response to salinity in transgenic canola plants.
authors.forename: Alireza
authors.initials: A
authors.lastname: Shafeinie
authors.forename: Valiollah
authors.initials: V
authors.lastname: Mohammadi
authors.forename: Houshang
authors.initials: H
authors.lastname: Alizadeh
authors.forename: Abas Ali
authors.initials: AA
authors.lastname: Zali
mesh_terms: D000222:Adaptation, Physiological; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001937:Brassica / Q000235:genetics / Q000502:physiology*; D017931:DNA Primers; D017343:Genes, Plant; D030821:Plants, Genetically Modified / Q000235:genetics / Q000502:physiology*; D016133:Polymerase Chain Reaction; D054712:Salinity; D013312:Stress, Physiological
chemical_list: D029681:Arabidopsis Proteins; D017931:DNA Primers