title: Transplant of ex vivo incubated bone marrow with rIL -7 for the enhancement of immuno-hematopoietic reconstitution.
abstract: Interleukin-7 (IL-7) is a critical cytokine in early B and T cell development. Peripheral T cell expansion and thymopoiesis and is a result of the ongoing reconstitution from uncommitted stem cells after transplant. We investigated the efficacy of ex vivo incubated bone marrow cells treated with recombinant human IL-7 (rIL-7) on subsequent in vivo murine models of syngeneic bone marrow (BM) transplant. After ex vivo culture with rIL-7, we observed a 1½-fold increase in BM cellularity; this increase was associated with an enhanced reconstitution of bone marrow cells and thymocytes at 45 days post-transplant. In addition to increased cellularity, lymphocytes from mice transplanted with cultured rIL-7 showed enhanced proliferative responses to mitogenic stimulation. These findings suggest rIL-7 to be a promising agent for the clinical application of treating immune deficiency and enhancing immuno-hematopoietic reconstitution of the stem cell auto/allograft.
authors.affiliation: a Department of Bone Marrow Transplantation & Cancer Immunotherapy , Hadassah/Hebrew University Medical Center , Kiryat Hadassah, Jerusalem , Israel.
authors.forename: Ali
authors.initials: A
authors.lastname: Abdul-Hai
authors.affiliation: b Department of Internal Medicine , Hadassah/Hebrew University Medical Center , Kiryat Hadassah, Jerusalem , Israel.
authors.forename: Aryeh
authors.initials: A
authors.lastname: Ben Yehuda
authors.affiliation: a Department of Bone Marrow Transplantation & Cancer Immunotherapy , Hadassah/Hebrew University Medical Center , Kiryat Hadassah, Jerusalem , Israel.
authors.forename: Shimon
authors.initials: S
authors.lastname: Slavin
authors.affiliation: a Department of Bone Marrow Transplantation & Cancer Immunotherapy , Hadassah/Hebrew University Medical Center , Kiryat Hadassah, Jerusalem , Israel.
authors.forename: Reuven
authors.initials: R
authors.lastname: Or
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D016026:Bone Marrow Transplantation / Q000379:methods; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D005260:Female; D015870:Gene Expression; D006410:Hematopoiesis / Q000187:drug effects* / Q000502:physiology*; D056747:Immunomodulation; D007376:Interleukin-2 / Q000494:pharmacology; D015851:Interleukin-7 / Q000494:pharmacology*; D051379:Mice; D023421:Models, Animal; D012333:RNA, Messenger; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D016176:T-Lymphocyte Subsets / Q000187:drug effects / Q000378:metabolism; D060168:Thymocytes / Q000187:drug effects / Q000378:metabolism; D019172:Transplantation Conditioning
chemical_list: D007376:Interleukin-2; D015851:Interleukin-7; D012333:RNA, Messenger; D011948:Receptors, Antigen, T-Cell
keywords: bone marrow transplant; immune reconstitution; interleukin-7 (IL-7); stem cell transplant