title: Molecular characterization and pathogenicity of a genogroup GVI feline norovirus.
abstract: Norovirus (NoV) has been classified into 6 genogroups, GI-GVI. In the present study, we identified novel feline NoV (FNoV) M49-1 strain. The C-terminal of RNA-dependent RNA polymerase of the FNoV M49-1 strain was highly homologous with GIV FNoV and GIV lion norovirus, whereas VP1 was highly homologous with GVI canine NoV (CNoV). Based on the results of the Simplot analysis, the FNoV M49-1 strain may have been produced by recombination between GIV.2 FNoV and GVI.1 CNoV. In addition, specific pathogen-free cats inoculated with FNoV gene-positive-fecal samples developed diarrhea symptoms, and the viral gene was detected in their feces and blood.
authors.affiliation: School of Veterinary Medicine, Kitasato University, Towada, Aomori, Japan.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Takano
authors.affiliation: Health and Environment Research Institute, Yokkaichi, Mie, Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Kusuhara
authors.affiliation: School of Veterinary Medicine, Kitasato University, Towada, Aomori, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Kuroishi
authors.affiliation: School of Veterinary Medicine, Kitasato University, Towada, Aomori, Japan.
authors.forename: Midori
authors.initials: M
authors.lastname: Takashina
authors.affiliation: School of Veterinary Medicine, Kitasato University, Towada, Aomori, Japan.
authors.forename: Tomoyoshi
authors.initials: T
authors.lastname: Doki
authors.affiliation: Health and Environment Research Institute, Yokkaichi, Mie, Japan.
authors.forename: Takamichi
authors.initials: T
authors.lastname: Nishinaka
authors.affiliation: School of Veterinary Medicine, Kitasato University, Towada, Aomori, Japan. Electronic address: hohdatsu@vmas.kitasato-u.ac.jp.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Hohdatsu
mesh_terms: D000818:Animals; D001483:Base Sequence; D017250:Caliciviridae Infections / Q000662:veterinary* / Q000821:virology; D002371:Cat Diseases / Q000821:virology*; D002415:Cats; D003967:Diarrhea / Q000662:veterinary; D005243:Feces / Q000821:virology; D005759:Gastroenteritis / Q000662:veterinary* / Q000821:virology; D005838:Genotype; D008969:Molecular Sequence Data; D029322:Norovirus / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000662:veterinary; D013047:Specific Pathogen-Free Organisms; D014774:Virulence
keywords: Enteric virus; Feline; Gastroenteritis; Genogroup; Norovirus