title: Development and validation of an LC-MS/MS method for the determination of bullatine A in rat plasma: application to a pharmacokinetic study.
abstract: Bullatine A is a diterpenoid alkaloid of Xue-Shang-Yi-Zhi-Hao (Aconitum brachypodum), which is widely used in traditional Chinese medicine for the treatment of rheumatism and pain. The plasma levels of bullatine A were measured by a rapid and sensitive LC-MS/MS method. Samples were prepared using acetonitrile precipitation and the separation of bullatine A was achieved on a Capcell Pak MG-C18 column by isocratic elution using acetonitrile (phase A) and 0.1% formic acid (phase B, pH 4.0; A:B, 30:70, v/v) as the mobile phase at a flow rate of 0.5 mL/min. Detection was performed on a triple-quadrupole tandem mass spectrometer by multiple-reaction monitoring of the transitions at m/z 344.2 → 105.2 for bullatine A and m/z 256.2 → 167.1 for the internal standard. The linearity was found to be within the concentration range of 1.32-440 ng/mL with a lower limit of quantification of 1.32 ng/mL. Only 1.3 min was needed for an each analytical run. This method was successfully applied in the determination of the active component bullatine A in rat plasma after intramuscular administration of A. brachypodum injection.
authors.affiliation: Department of Anesthesiology, the First Hospital of Jilin University, Changchun, 130021, People's Republic of China.
authors.forename: Shi-Yong
authors.initials: SY
authors.lastname: Teng
authors.affiliation: Department of Pharmacy, the First Hospital of Jilin University, Changchun, 130021, People's Republic of China.
authors.forename: Si-Xi
authors.initials: SX
authors.lastname: Zhang
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, the First Hospital of Jilin University, Changchun, 130021, People's Republic of China.
authors.forename: Kai
authors.initials: K
authors.lastname: Niu
authors.affiliation: Department of Medicine Management, the Second Hospital of Jilin University, Changchun, 130041, People's Republic of China.
authors.forename: Li-Jie
authors.initials: LJ
authors.lastname: Zhai
authors.affiliation: Department of Critical Care Medicine, the First Hospital of Jilin University, Changchun, 130021, People's Republic of China.
authors.forename: Shi-Ji
authors.initials: SJ
authors.lastname: Wang
mesh_terms: D000470:Alkaloids / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004224:Diterpenes / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D016014:Linear Models; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000470:Alkaloids; D004224:Diterpenes; C587049:bullatine A
keywords: LC-MS/MS; bullatine A; pharmacokinetics; rat plasma