title: Extraction optimization, characterization and antioxidant activity in vitro of polysaccharides from mulberry (Morus alba L.) leaves.
abstract: Extraction optimization, characterization and antioxidant activity in vitro of polysaccharides from mulberry leaves (MLP) were investigated in the present study. The optimal extraction conditions with an extraction yield of 10.0 ± 0.5% for MLP were determined as follows: extraction temperature 92 °C, extraction time 3.5h and ratio (v/w, mL/g) of extraction solvent (water) to raw material 34. Two purified fractions, MLP-3a and MLP-3b with molecular weights of 80.99 and 3.64 kDa, respectively, were obtained from crude MLP by chromatography of DEAE-Cellulose 52 and Sephadex G-100. Fourier transform-infrared spectroscopy revealed that crude MLP, MLP-3a and MLP-3b were acidic polysaccharides. Furthermore, crude MLP and MLP-3a had more complicated monosaccharide compositions, while MLP-3b had a relatively higher content of uronic acid. Crude MLP, MLP-3a and MLP-3b exhibited potent Fe(2+) chelating power and scavenging activities on 1,1-diphenyl-2-picrylhydrazyl, hydroxyl, superoxide and 2,2'-azinobis-(3-ethyl-benzothiazolin-6-sulfonic acid) radicals. The results suggested that MLP could be explored as natural antioxidant.
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.
authors.forename: Qingxia
authors.initials: Q
authors.lastname: Yuan
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Xie
authors.affiliation: College of Life Sciences and Laboratory Center of Life Sciences, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Yan
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Ye
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China; Institute of Food Science and Nutrition, University of Sargodha, Sargodha, Pakistan.
authors.forename: Saqib
authors.initials: S
authors.lastname: Jabbar
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China. Electronic address: zengxx@njau.edu.cn.
authors.forename: Xiaoxiong
authors.initials: X
authors.lastname: Zeng
mesh_terms: D000975:Antioxidants / Q000032:analysis / Q000737:chemistry*; D052160:Benzothiazoles / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D017665:Hydroxyl Radical / Q000737:chemistry; D009005:Monosaccharides / Q000032:analysis; D031625:Morus; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D010940:Plant Proteins / Q000032:analysis; D059808:Polyphenols / Q000032:analysis; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry*; D013451:Sulfonic Acids / Q000737:chemistry; D013481:Superoxides / Q000737:chemistry; D014574:Uronic Acids / Q000032:analysis
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D009005:Monosaccharides; D010851:Picrates; D010936:Plant Extracts; D010940:Plant Proteins; D059808:Polyphenols; D011134:Polysaccharides; D013451:Sulfonic Acids; D014574:Uronic Acids; D013481:Superoxides; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Antioxidant; Characterization; Extraction; Mulberry leaf; Polysaccharide