title: Fractionation of yeast extract by nanofiltration process to assess key compounds involved in CHO cell culture improvement.
abstract: Yeast extract (YE) is known to greatly enhance mammalian cell culture performances, but its undefined composition decreases process reliability. Accordingly, in the present study, the nature of YE compounds involved in the improvement of recombinant CHO cell growth and IgG production was investigated. First, the benefits of YE were verified, revealing that it increased maximal concentrations of viable cells and IgG up to 73 and 60%, respectively compared to a reference culture. Then, the analyses of YE composition highlighted the presence of molecules such as amino acids, vitamins, salts, nucleobase, and glucose that were contained in reference medium, while others including peptides, trehalose, polysaccharides, and nucleic acids were not. Consequently, YE was fractionated by a nanofiltration process to deeper evaluate its effects on CHO cell cultures. The YE molecules already contained in reference medium were mainly isolated in the permeate fraction together with trehalose and short peptides, while other molecules were concentrated in the retentate. Permeate, which was free of macromolecules, exhibited a similar positive effect than raw YE on maximal concentrations. Additional studies on cell energetic metabolism underlined that dipeptides and tripeptides in permeate were used as an efficient source of nitrogenous substrates.
authors.affiliation: Laboratoire Réaction et Génie des Procédés, CNRS-UMR-7274, plateforme SVS, 13 rue du bois de la Champelle, F-54500, Vandœuvre-lès-Nancy, France.|Université de Lorraine, UMR-7274, ENSAIA, 2 avenue de la forêt de Haye, TSA 40602, F-54518, Vandœuvre-lès-Nancy, France.|BioSpringer, 103 rue Jean Jaurès, F-94704, Maisons-Alfort, France.
authors.forename: Mathilde
authors.initials: M
authors.lastname: Mosser
authors.affiliation: Laboratoire Réaction et Génie des Procédés, CNRS-UMR-7274, plateforme SVS, 13 rue du bois de la Champelle, F-54500, Vandœuvre-lès-Nancy, France.|Université de Lorraine, UMR-7274, ENSAIA, 2 avenue de la forêt de Haye, TSA 40602, F-54518, Vandœuvre-lès-Nancy, France.
authors.forename: Romain
authors.initials: R
authors.lastname: Kapel
authors.affiliation: Laboratoire Réaction et Génie des Procédés, CNRS-UMR-7274, plateforme SVS, 13 rue du bois de la Champelle, F-54500, Vandœuvre-lès-Nancy, France.|Université de Lorraine, UMR-7274, ENSAIA, 2 avenue de la forêt de Haye, TSA 40602, F-54518, Vandœuvre-lès-Nancy, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Chevalot
authors.affiliation: Laboratoire Réaction et Génie des Procédés, CNRS-UMR-7274, plateforme SVS, 13 rue du bois de la Champelle, F-54500, Vandœuvre-lès-Nancy, France.|Université de Lorraine, UMR-7274, ENSAIA, 2 avenue de la forêt de Haye, TSA 40602, F-54518, Vandœuvre-lès-Nancy, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Olmos
authors.affiliation: Laboratoire Réaction et Génie des Procédés, CNRS-UMR-7274, plateforme SVS, 13 rue du bois de la Champelle, F-54500, Vandœuvre-lès-Nancy, France.|Université de Lorraine, UMR-7274, ENSAIA, 2 avenue de la forêt de Haye, TSA 40602, F-54518, Vandœuvre-lès-Nancy, France.
authors.forename: Ivan
authors.initials: I
authors.lastname: Marc
authors.affiliation: Laboratoire Réaction et Génie des Procédés, CNRS-UMR-7274, plateforme SVS, 13 rue du bois de la Champelle, F-54500, Vandœuvre-lès-Nancy, France.|Université de Lorraine, UMR-7274, ENSAIA, 2 avenue de la forêt de Haye, TSA 40602, F-54518, Vandœuvre-lès-Nancy, France.
authors.forename: Annie
authors.initials: A
authors.lastname: Marc
authors.affiliation: BioSpringer, 103 rue Jean Jaurès, F-94704, Maisons-Alfort, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Oriol
mesh_terms: D000818:Animals; D016466:CHO Cells; D002457:Cell Extracts / Q000737:chemistry*; D006224:Cricetinae; D003412:Cricetulus; D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism*; D005374:Filtration / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis / Q000378:metabolism; D007700:Kinetics; D036103:Nanotechnology / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000737:chemistry*
chemical_list: D002457:Cell Extracts; D003470:Culture Media; D007074:Immunoglobulin G
keywords: CHO cells culture; IgG production; kinetics studies; nanofiltration; yeast extract