title: Sage (Salvia officinalis) and fennel (Foeniculum vulgare) improve cryopreserved boar epididymal semen quality study.
abstract: The aim of this study was to evaluate the effect of fennel and sage extracts and the influence of the egg yolk source (fresh or pasteurized) on the success of freezing boar epididymal spermatozoa. In experiment 1, epididymal sperm was recovered by flushing and cryopreserved in a lactose-egg yolk solution supplemented with various concentrations (10, 5 and 2.5 g/L) of sage or fennel. Sperm quality was evaluated (motility, viability, HOST and acrosome integrity) at 0 h and 2 h after thawing. Fennel 10 g/L and sage 5 g/L and control (no extracts) were selected for experiment 2 which also compared fresh or pasteurized egg yolk in the freezing extender and measured DNA integrity of the frozen sperm. Results showed that the interaction between fennel and sage antioxidants with fresh egg yolk significantly improved post thaw sperm quality and protected boar epididymal spermatozoa from cryopreservation damage as a result of oxidative stress.
authors.affiliation: Department of Animal Pathology, Obstetrics and Reproduction Area, Faculty of Veterinary, University of Zaragoza, Zaragoza, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Monton
authors.affiliation: Department of Animal Pathology, Obstetrics and Reproduction Area, Faculty of Veterinary, University of Zaragoza, Zaragoza, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Gil
authors.affiliation: Department of Animal Pathology, Obstetrics and Reproduction Area, Faculty of Veterinary, University of Zaragoza, Zaragoza, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Malo
authors.affiliation: Department of Animal Pathology, Obstetrics and Reproduction Area, Faculty of Veterinary, University of Zaragoza, Zaragoza, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Olaciregui
authors.affiliation: Department of Animal Pathology, Obstetrics and Reproduction Area, Faculty of Veterinary, University of Zaragoza, Zaragoza, Spain.
authors.forename: N
authors.initials: N
authors.lastname: Gonzalez
authors.affiliation: Department of Animal Pathology, Infectious Diseases Area, Faculty of Veterinary, University of Zaragoza, Zaragoza, Spain.
authors.forename: I
authors.initials: I
authors.lastname: de Blas
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000378:metabolism*; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000302:isolation & purification / Q000378:metabolism; D004530:Egg Yolk / Q000378:metabolism; D030001:Foeniculum / Q000737:chemistry*; D008297:Male; D010936:Plant Extracts / Q000302:isolation & purification / Q000378:metabolism*; D027543:Salvia officinalis / Q000737:chemistry*; D012661:Semen / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D055101:Semen Analysis; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013552:Swine / Q000378:metabolism
chemical_list: D000975:Antioxidants; D003451:Cryoprotective Agents; D010936:Plant Extracts