title: Fibroblast Migration in 3D is Controlled by Haptotaxis in a Non-muscle Myosin II-Dependent Manner.
abstract: Cell migration in 3D is a key process in many physiological and pathological processes. Although valuable knowledge has been accumulated through analysis of various 2D models, some of these insights are not directly applicable to migration in 3D. In this study, we have confined biomimetic hydrogels within microfluidic platforms in the presence of a chemoattractant (platelet-derived growth factor-BB). We have characterized the migratory responses of human fibroblasts within them, particularly focusing on the role of non-muscle myosin II. Our results indicate a prominent role for myosin II in the integration of chemotactic and haptotactic migratory responses of fibroblasts in 3D confined environments.
authors.affiliation: Multiscale in Mechanical and Biological Engineering (M2BE), Department of Mechanical Engineering, Aragon Institute of Engineering Research (I3A), University of Zaragoza, 50018, Saragossa, Spain.
authors.forename: O
authors.initials: O
authors.lastname: Moreno-Arotzena
authors.affiliation: Multiscale in Mechanical and Biological Engineering (M2BE), Department of Mechanical Engineering, Aragon Institute of Engineering Research (I3A), University of Zaragoza, 50018, Saragossa, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Borau
authors.affiliation: Multiscale in Mechanical and Biological Engineering (M2BE), Department of Mechanical Engineering, Aragon Institute of Engineering Research (I3A), University of Zaragoza, 50018, Saragossa, Spain.
authors.forename: N
authors.initials: N
authors.lastname: Movilla
authors.affiliation: Department of Medicine, Hospital Universitario de la Princesa, Universidad Autonoma de Madrid School of Medicine, 28006, Madrid, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Vicente-Manzanares
authors.affiliation: Multiscale in Mechanical and Biological Engineering (M2BE), Department of Mechanical Engineering, Aragon Institute of Engineering Research (I3A), University of Zaragoza, 50018, Saragossa, Spain. jmgaraz@unizar.es.
authors.forename: J M
authors.initials: JM
authors.lastname: García-Aznar
mesh_terms: D000077214:Becaplermin; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D020100:Hydrogels; D044085:Microfluidics; D024462:Myosin Type II / Q000502:physiology*; D020574:Proto-Oncogene Proteins c-sis / Q000494:pharmacology
chemical_list: D020100:Hydrogels; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D024462:Myosin Type II
keywords: Chemotaxis; Collagen; Fibrin; Hydrogel; Mechanical properties; Microfluidics