title: Identification and analysis of polyaromatic hydrocarbons (PAHs)--biodegrading bacterial strains from refinery soil of India.
abstract: Polyaromatic hydrocarbons (PAHs) utilizing bacteria were isolated from soils of seven sites of Mathura refinery, India. Twenty-six bacterial strains with different morphotypes were isolated. These strains were acclimatized to utilize a mixture of four polycyclic aromatic hydrocarbons, i.e., anthracene, fluorene, phenanthrene, and pyrene, each at 50 mg/L concentration as sole carbon source. Out of total isolates, 15 potent isolates were subjected to 16S rDNA sequencing and identified as a member of diverse genera, i.e., Bacillus, Acinetobacter, Stenotrophomonas, Alcaligenes, Lysinibacillus, Brevibacterium, Serratia, and Streptomyces. Consortium of four promising isolates (Acinetobacter, Brevibacterium, Serratia, and Streptomyces) were also investigated for bioremediation of PAH mixture. This consortium was proved to be efficient PAH degrader resulting in 40-70 % degradation of PAH within 7 days. Results of this study indicated that these genera may play an active role in bioremediation of PAHs.
authors.affiliation: Division of Microbiology, Indian Agricultural Research Institute, New Delhi, 110012, India.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Chaudhary
authors.forename: Harmesh
authors.initials: H
authors.lastname: Sahay
authors.forename: Richa
authors.initials: R
authors.lastname: Sharma
authors.forename: Alok Kumar
authors.initials: AK
authors.lastname: Pandey
authors.forename: Shashi Bala
authors.initials: SB
authors.lastname: Singh
authors.forename: A K
authors.initials: AK
authors.lastname: Saxena
authors.forename: Lata
authors.initials: L
authors.lastname: Nain
mesh_terms: D001407:Bacillus / Q000302:isolation & purification; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D001673:Biodegradation, Environmental; D004784:Environmental Monitoring; D019652:Extraction and Processing Industry; D005449:Fluorenes; D007194:India; D059392:Petroleum Pollution; D010616:Phenanthrenes; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000378:metabolism*; D011721:Pyrenes; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism*
chemical_list: D005449:Fluorenes; D010616:Phenanthrenes; D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes; D012987:Soil; D012989:Soil Pollutants; C041509:fluorene; C031181:phenanthrene; C030984:pyrene