title: HIF3A association with adiposity: the story begins before birth.
abstract: OBJECTIVE
  Determine if the association of HIF3A DNA methylation with weight and adiposity is detectable early in life.
  
  
  METHODS
  We determined HIF3A genotype and DNA methylation patterns (on hybridization arrays) in DNA extracted from umbilical cords of 991 infants. Methylation levels at three CpGs in the HIF3A first intron were related to neonatal and infant anthropometry and to genotype at nearby polymorphic sites.
  
  
  CONCLUSIONS
  Higher methylation levels at three previously described HIF3A CpGs were associated with greater infant weight and adiposity. The effect sizes were slightly smaller than those reported for adult BMI. There was also an interaction within cis-genotype. The association between higher DNA methylation at HIF3A and increased adiposity is present in neonates. In this study, no particular prenatal factor strongly influenced HIF3A hypermethylation. Our data nonetheless suggest shared prenatal influences on HIF3A methylation and adiposity.
authors.affiliation: Singapore Institute for Clinical Sciences (SICS), A*STAR, Brenner Centre for Molecular Medicine, 117609, Singapore.|School of Computer Engineering, Nanyang Technological University (NTU), 639798, Singapore.
authors.forename: Hong
authors.initials: H
authors.lastname: Pan
authors.affiliation: Singapore Institute for Clinical Sciences (SICS), A*STAR, Brenner Centre for Molecular Medicine, 117609, Singapore.
authors.forename: Xinyi
authors.initials: X
authors.lastname: Lin
authors.affiliation: Singapore Institute for Clinical Sciences (SICS), A*STAR, Brenner Centre for Molecular Medicine, 117609, Singapore.
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Singapore Institute for Clinical Sciences (SICS), A*STAR, Brenner Centre for Molecular Medicine, 117609, Singapore.
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.affiliation: Singapore Institute for Clinical Sciences (SICS), A*STAR, Brenner Centre for Molecular Medicine, 117609, Singapore.
authors.forename: Ai Ling
authors.initials: AL
authors.lastname: Teh
authors.affiliation: Saw Swee Hock School of Public Health, National University of Singapore (NUS), 117597, Singapore.|Yong Loo Lin School of Medicine, National University of Singapore (NUS), 119228, Singapore.
authors.forename: Shu E
authors.initials: SE
authors.lastname: Soh
authors.affiliation: Singapore Institute for Clinical Sciences (SICS), A*STAR, Brenner Centre for Molecular Medicine, 117609, Singapore.|Yong Loo Lin School of Medicine, National University of Singapore (NUS), 119228, Singapore.|Division of Paediatric Endocrinology & Diabetes, Khoo Teck Puat-National University Children's Medical Institute, National University Health System, 119228, Singapore.
authors.forename: Yung Seng
authors.initials: YS
authors.lastname: Lee
authors.affiliation: Yong Loo Lin School of Medicine, National University of Singapore (NUS), 119228, Singapore.
authors.forename: Mya Thway
authors.initials: MT
authors.lastname: Tint
authors.affiliation: Department of Medical Genetics, Centre for Molecular Medicine & Therapeutics, Child & Family Research Institute, University of British Columbia, Vancouver, BC, V5Z 4H4 Canada.
authors.forename: Julia L
authors.initials: JL
authors.lastname: MacIsaac
authors.affiliation: Department of Medical Genetics, Centre for Molecular Medicine & Therapeutics, Child & Family Research Institute, University of British Columbia, Vancouver, BC, V5Z 4H4 Canada.
authors.forename: Alexander M
authors.initials: AM
authors.lastname: Morin
authors.affiliation: KK Women's and Children's Hospital, 229899, Singapore.
authors.forename: Kok Hian
authors.initials: KH
authors.lastname: Tan
authors.affiliation: KK Women's and Children's Hospital, 229899, Singapore.
authors.forename: Fabian
authors.initials: F
authors.lastname: Yap
authors.affiliation: Saw Swee Hock School of Public Health, National University of Singapore (NUS), 117597, Singapore.
authors.forename: Seang Mei
authors.initials: SM
authors.lastname: Saw
authors.affiliation: Department of Medical Genetics, Centre for Molecular Medicine & Therapeutics, Child & Family Research Institute, University of British Columbia, Vancouver, BC, V5Z 4H4 Canada.
authors.forename: Michael S
authors.initials: MS
authors.lastname: Kobor
authors.affiliation: Singapore Institute for Clinical Sciences (SICS), A*STAR, Brenner Centre for Molecular Medicine, 117609, Singapore.|Ludmer Centre for Neuroinformatics & Mental Health, Douglas University Mental Health Institute, McGill University, Montreal, (Quebec) H4H 1R3, Canada.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Meaney
authors.affiliation: MRC Lifecourse Epidemiology Unit & NIHR Southampton Biomedical Research Centre, University of Southampton & University Hospital Southampton NHS Foundation Trust, Southampton, SO16 6YD, UK.
authors.forename: Keith M
authors.initials: KM
authors.lastname: Godfrey
authors.affiliation: Singapore Institute for Clinical Sciences (SICS), A*STAR, Brenner Centre for Molecular Medicine, 117609, Singapore.|Yong Loo Lin School of Medicine, National University of Singapore (NUS), 119228, Singapore.
authors.forename: Yap-Seng
authors.initials: YS
authors.lastname: Chong
authors.affiliation: Singapore Institute for Clinical Sciences (SICS), A*STAR, Brenner Centre for Molecular Medicine, 117609, Singapore.|Centre for Human Evolution, Adaptation & Disease, Liggins Institute, University of Auckland, Auckland, 1142, New Zealand.
authors.forename: Peter D
authors.initials: PD
authors.lastname: Gluckman
authors.affiliation: Singapore Institute for Clinical Sciences (SICS), A*STAR, Brenner Centre for Molecular Medicine, 117609, Singapore.|Yong Loo Lin School of Medicine, National University of Singapore (NUS), 119228, Singapore.
authors.forename: Neerja
authors.initials: N
authors.lastname: Karnani
authors.affiliation: Singapore Institute for Clinical Sciences (SICS), A*STAR, Brenner Centre for Molecular Medicine, 117609, Singapore.
authors.forename: Joanna D
authors.initials: JD
authors.lastname: Holbrook
mesh_terms: D050154:Adiposity / Q000235:genetics*; D000328:Adult; D000483:Alleles; D051017:Apoptosis Regulatory Proteins; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics*; D001724:Birth Weight; D002675:Child, Preschool; D018899:CpG Islands; D019175:DNA Methylation; D004781:Environmental Exposure; D044127:Epigenesis, Genetic; D005260:Female; D005786:Gene Expression Regulation; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D018811:Maternal Exposure; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D012097:Repressor Proteins; D014470:Umbilical Cord / Q000378:metabolism
chemical_list: D051017:Apoptosis Regulatory Proteins; D051792:Basic Helix-Loop-Helix Proteins; D012097:Repressor Proteins; C116973:HIF3A protein, human
keywords: DNA methylation; HIF3A protein; birth weight; embryonic and fetal development; epigenomics; human; obesity