title: Brain metabolomics in rats after administration of ketamine.
abstract: In this study, we developed a brain metabolomic method, based on gas chromatography-mass spectrometry (GC/MS), to evaluate the effect of ketamine on rats. Pattern recognition analysis, including both principal component analysis and partial least squares-discriminate analysis revealed that ketamine induced metabolic perturbations. Compared with the control group, the levels of glycerol, uridine, cholesterol in rat brain of the ketamine group (50 mg/kg, 14 days) decreased, while the urea levels increased. Our results indicate that metabolomic methods based on GC/MS may be useful to elucidate ketamine abuse through the exploration of biomarkers.
authors.affiliation: Laboratory Animal Centre of Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Congcong
authors.initials: C
authors.lastname: Wen
authors.affiliation: Analytical and Testing Center of Wenzhou Medical University, Wenzhou, 325035, China.|Forensic Toxicology Laboratory of Forensic Center, Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Analytical and Testing Center of Wenzhou Medical University, Wenzhou, 325035, China.|Forensic Toxicology Laboratory of Forensic Center, Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Analytical and Testing Center of Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Fa
authors.initials: F
authors.lastname: Sun
authors.affiliation: Analytical and Testing Center of Wenzhou Medical University, Wenzhou, 325035, China.|Forensic Toxicology Laboratory of Forensic Center, Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Jianshe
authors.initials: J
authors.lastname: Ma
authors.affiliation: The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China.
authors.forename: Lufeng
authors.initials: L
authors.lastname: Hu
authors.affiliation: The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China.
authors.forename: Guanyang
authors.initials: G
authors.lastname: Lin
authors.affiliation: Analytical and Testing Center of Wenzhou Medical University, Wenzhou, 325035, China.|Forensic Toxicology Laboratory of Forensic Center, Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Xianqin
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D001921:Brain / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007649:Ketamine / Q000008:administration & dosage / Q000378:metabolism*; D008297:Male; D055432:Metabolomics / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D015415:Biomarkers; D007649:Ketamine
keywords: GC/MS; abuse; ketamine; metabolomics