title: Description of Geodermatophilus bullaregiensis sp. nov.
abstract: The taxonomic position of an aerobic actinobacterial strain, BMG841(T), isolated from the Bulla Regia monument (Tunisia) and exhibiting a high resistance to gamma-radiation (D10 ~9 kGy) was determined using polyphasic approach. The optimal growth range was found to be 25-35 °C at pH of 7.0-8.5. The strain was observed to form black dry colonies. Chemotaxonomic characteristics of the isolate showed a cell wall type III, with galactose and glucose as diagnostic sugars; phosphatidylcholine, phosphatidylinositol, diphosphatidylglycerol, phosphatidylethanolamine and an unidentified glycolipid as main polar lipids; and MK-9(H4) as the predominant menaquinone. The major cellular fatty acids were identified as iso-C16:0 and iso-C15:0. Phylogenetic analysis indicated that strain BMG841(T) represents a novel member of the genus Geodermatophilus with high 16S rRNA gene sequence identity with Geodermatophilus saharensis (98.28 %). Based on phylogenetic and phenotypic analysis, strain BMG841(T) is proposed as the type strain (=DSM 46841(T) = CECT 8821(T)) of a novel species, Geodermatophilus bullaregiensis.
authors.affiliation: Laboratoire Microorganismes et Biomolécules Actives, Université de Tunis El Manar (FST) & Université de Carthage (INSAT), 2092, Tunis, Tunisia.
authors.forename: Karima
authors.initials: K
authors.lastname: Hezbri
authors.forename: Faten
authors.initials: F
authors.lastname: Ghodhbane-Gtari
authors.forename: Maria
authors.initials: M
authors.lastname: Del Carmen Montero-Calasanz
authors.forename: Haïtham
authors.initials: H
authors.lastname: Sghaier
authors.forename: Manfred
authors.initials: M
authors.lastname: Rohde
authors.forename: Peter
authors.initials: P
authors.lastname: Schumann
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Klenk
authors.forename: Maher
authors.initials: M
authors.lastname: Gtari
mesh_terms: D039903:Actinobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D002241:Carbohydrates / Q000032:analysis; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004783:Environmental Microbiology; D005227:Fatty Acids / Q000032:analysis; D006017:Glycolipids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013696:Temperature; D014416:Tunisia; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D002241:Carbohydrates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D006017:Glycolipids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2