title: Characterization and tissue distribution of conjugated metabolites of pyrene in the rat.
abstract: Pyrene (PY) is a polycyclic aromatic hydrocarbon (PAH) that is often used as a biomarker for human and wildlife exposure to PAHs. As the metabolites of PAHs, similar to their parent compounds, pose public health risks, it is necessary to study their characteristics and tissue-specific distribution. The present study was performed to experimentally characterize PY metabolites and analyze the tissue-specific distribution of the conjugated metabolites after oral administration of PY to rats. PY metabolites, such as pyrenediol-disulfate (PYdiol-diS), pyrenediol-sulfate (PYdiol-S), pyrene-1-sulfate (PYOS), pyrene-1-glucuronide (PYOG) and 1-hydroxypyrene (PYOH), were detected in rat urine. Although glucuronide conjugate was the predominant metabolite, the metabolite composition varied among tissues. Interestingly, the proportion of PYOH was high in the large intestine. Furthermore, PYOH was the only PY metabolite detected in feces.
authors.affiliation: Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, N18 W9, Kita-ku, Sapporo 060-0818, Japan.
authors.forename: Aksorn
authors.initials: A
authors.lastname: Saengtienchai
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Ikenaka
authors.forename: Wageh Sobhy
authors.initials: WS
authors.lastname: Darwish
authors.forename: Shouta M M
authors.initials: SM
authors.lastname: Nakayama
authors.forename: Hazuki
authors.initials: H
authors.lastname: Mizukawa
authors.forename: Mayumi
authors.initials: M
authors.lastname: Ishizuka
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D004064:Digestive System / Q000737:chemistry / Q000378:metabolism; D004785:Environmental Pollutants / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D005243:Feces / Q000737:chemistry; D007668:Kidney / Q000737:chemistry / Q000378:metabolism; D008168:Lung / Q000737:chemistry / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000378:metabolism; D011721:Pyrenes / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D051381:Rats; D017208:Rats, Wistar; D013737:Testis / Q000737:chemistry / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D004785:Environmental Pollutants; D011721:Pyrenes; C030984:pyrene