title: Adsorption of bovine serum albumin onto synthetic Fe-doped geomimetic chrysotile.
abstract: Synthetic stoichiometric and Fe-doped geomimetic chrysotile nanocrystals represent a reference standard to investigate the health hazard associated with mineral asbestos fibres. Experimental evidence suggests that the generation of reactive oxygen species and other radicals, catalysed by iron ions at the fibre surface, plays an important role in asbestos-induced cytotoxicity and genotoxicity. In this study, structural modification of bovine serum albumin (BSA) adsorbed onto synthetic chrysotile doped with different amounts of Fe has been investigated by Fourier transform infrared spectroscopy (FT-IR), thermal gravimetric analysis (TGA) and analytical pyrolysis coupled with gas chromatography-mass spectrometry. FT-IR data evidenced a marked increase in disordered structures like random coil and β-turn of BSA-nanocrystal adduct with 0.52 wt% of Fe doped. The TGA profile of the BSA revealed that its interaction with the synthetic chrysotile surface was strongly affected by the substitution of Fe into the chrysotile structure. The 2,5-diketopiperazine yields, formed upon thermal degradation of the polypeptide chain (pyrolysis-gas chromatography), changed when the BSA was adsorbed on the nanofibres. In general, results suggested that minute amount (less than 1 wt%) of Fe doping in chrysotile affected the protein-nanofibre interactions, supporting the role that this element may play in asbestos toxicity. The catalytic role of iron and the consequent unfolding of protein due to the structural surface modification of nanofibres were also evaluated.
authors.affiliation: Department of Chemistry 'G. Ciamician', Alma Mater Studiorum University of Bologna, via Selmi 2, 40126 Bologna, Italy Centro Interdipartimentale di Ricerca in Scienze Ambientali Sede di Ravenna, Università di Bologna, via S. Alberto 163, 48100 Ravenna, Italy.
authors.forename: Alessio
authors.initials: A
authors.lastname: Adamiano
authors.affiliation: Department of Chemistry 'G. Ciamician', Alma Mater Studiorum University of Bologna, via Selmi 2, 40126 Bologna, Italy isidorogiorgio.lesci@unibo.it.
authors.forename: Isidoro Giorgio
authors.initials: IG
authors.lastname: Lesci
authors.affiliation: Department of Chemistry 'G. Ciamician', Alma Mater Studiorum University of Bologna, via Selmi 2, 40126 Bologna, Italy Centro Interdipartimentale di Ricerca in Scienze Ambientali Sede di Ravenna, Università di Bologna, via S. Alberto 163, 48100 Ravenna, Italy.
authors.forename: Daniele
authors.initials: D
authors.lastname: Fabbri
authors.affiliation: Department of Chemistry 'G. Ciamician', Alma Mater Studiorum University of Bologna, via Selmi 2, 40126 Bologna, Italy.
authors.forename: Norberto
authors.initials: N
authors.lastname: Roveri
mesh_terms: D000327:Adsorption; D000818:Animals; D017632:Asbestos, Serpentine / Q000737:chemistry*; D002417:Cattle; D007501:Iron / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D017632:Asbestos, Serpentine; D012710:Serum Albumin, Bovine; D007501:Iron
keywords: 2,5-diketopiperazines; Fe surface interaction; analytical pyrolysis/gas chromatography–mass spectrometry; chrysotile–bovine serum albumin adduct; synthetic Fe-doped chrysotile