title: [Triptolide induces apoptosis of human acute T lymphocytic leukemia Jurkat cells via inhibiting transcription of human endogenous retrovirus HERV-K Np9 gene].
abstract: OBJECTIVE
  To investigate the molecular?mechanisms by which triptolide induces apoptosis of human acute T lymphocytic leukemia Jurkat cells.
  
  
  METHODS
  MTT assay was employed to detect the proliferation inhibition of Jurkat cells by triptolide, and the IC50 was calculated by OriginPro8. Flow cytometry was used to analyze apoptosis of Jurkat cells. Np9 mRNA levels were detected by RT-PCR and analyzed quantitatively by Kodak 1D 3.6 software. Correlation between the inhibition of Np9 transcription and the cell apoptosis was analyzed by SPSS 19.0.Western blotting was employed to determine Np9 downstream signaling molecules c-myc, β-catenin, ERK, AKT and Notch1 protein level in Jurkat cells after exposure to different concentrations of triptolide for 48 h.
  
  
  RESULTS
  Triptolide treatment resulted in dose-dependent inhibition of Jurkat cells proliferation and its IC50 was 12.7 nmol/L. Triptolide induced apoptosis of Jurkat cells in dose- dependent manner. Furthermore, triptolide inhibited Np9 mRNA transcription level in Jurakt cells in a dose-dependent manner. There was a correlation between the triptolide-mediated the apoptosis and the inhibition of Np9 transcription of Jurkat cells (R(2)=0.907). Western blotting results displayed that triptolide inhibited transcription levels of Np9 mRNA with a concomitant decrease of its downstream signaling molecules c-myc, β-catenin, ERK, AKT and Notch1 at protein levels.
  
  
  CONCLUSIONS
  Inhibition of HERV-K Np9 mRNA and its downstream signaling molecules c-myc, β-catenin, ERK, Akt and Notch1 protein might be one of important molecular?mechanisms by which triptolide induces apoptosis of human acute T lymphocytic leukemia Jurkat cells.
authors.affiliation: Department of Hematology, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China.E-mail: chen2007jiang@163.com.
authors.forename: Jianghua
authors.initials: J
authors.lastname: Chen
authors.forename: Weiwei
authors.initials: W
authors.lastname: Zheng
authors.forename: Xudong
authors.initials: X
authors.lastname: Jiang
authors.forename: Xiaoya
authors.initials: X
authors.lastname: Lu
authors.forename: Rongzhen
authors.initials: R
authors.lastname: Xu
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D004224:Diterpenes / Q000494:pharmacology*; D020077:Endogenous Retroviruses / Q000235:genetics; D004852:Epoxy Compounds / Q000494:pharmacology; D005434:Flow Cytometry; D015686:Gene Products, env / Q000235:genetics*; D006801:Humans; D019169:Jurkat Cells / Q000187:drug effects; D010616:Phenanthrenes / Q000494:pharmacology*; D054218:Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / Q000473:pathology*; D014158:Transcription, Genetic
chemical_list: D004224:Diterpenes; D004852:Epoxy Compounds; D015686:Gene Products, env; C469430:Np9 protein, HERV-K, human; D010616:Phenanthrenes; C001899:triptolide