title: Comparative Studies on Polyphenolic Composition, Antioxidant and Diuretic Effects of Nigella sativa L. (Black Cumin) and Nigella damascena L. (Lady-in-a-Mist) Seeds.
abstract: This study was performed to evaluate the phenolic profile, antioxidant and diuretic effects of black cumin and lady-in-a-mist seeds. In the phenolic profile, differences between the two species are significant. Qualitative and quantitative analyses of the phenolic compounds were performed using a HPLC-UV/MS method. Hyperoside was the only identified flavonoid glycoside (1.08 ± 0.01 μg∙g-1 dw plant material), in the N. damascena extract. Regarding the flavonol profile, kaempferol was identified before the hydrolysis, only in the N. sativa extract (6.06 ± 0.02 μg∙g-1 dw plant material) and quercetin only in N. damascena seeds (14.35 ± 0.02 μg∙g-1 dw plant material). The antioxidant potential of the two species was tested through several electron transfer assays, which indicated, excepting for the FRAP assay, N. damascena as exhibiting a higher free radical scavenging activity. The diuretic activity of the two extracts was tested using a rat-experimental model on acute diuresis. Administration of the ethanolic extract of N. sativa (100 mg∙kg-1) resulted in a significant increase in urine volume, although less than found with the reference drug; in addition N. damascena extract did not present a diuretic effect. In reference to the elimination of Na+, K+ and uric acid, the black cumin extract exhibited a higher natriuretic than kaluretic effect and a similar uricosuric effect with control and N. damascena. For N. damascena, the Na+/K+ ratio was sub unitary, but not due to an increasing of the kaluretic effect, but mostly to a decrease of Na+ excretion.
authors.affiliation: Faculty of Medicine, Pharmacy and Dental Medicine, Vasile Goldiș Western University of Arad, 86, Rebreanu Street, Arad 310414, Romania. claudiatoma@uvvg.com.
authors.forename: Claudia-Crina
authors.initials: CC
authors.lastname: Toma
authors.affiliation: Faculty of Medicine, Pharmacy and Dental Medicine, Vasile Goldiș Western University of Arad, 86, Rebreanu Street, Arad 310414, Romania. neliolah@yahoo.com.|SC PlantExtrakt SRL, Rădaia 407059, Cluj, Romania. neliolah@yahoo.com.
authors.forename: Neli-Kinga
authors.initials: NK
authors.lastname: Olah
authors.affiliation: Faculty of Pharmacy, Iuliu Hațieganu University of Medicine and Pharmacy, 8, V. Babes Street, Cluj-Napoca 400010, Romania. laurian.vlase@umfcluj.ro.
authors.forename: Laurian
authors.initials: L
authors.lastname: Vlase
authors.affiliation: Faculty of Pharmacy, Iuliu Hațieganu University of Medicine and Pharmacy, 8, V. Babes Street, Cluj-Napoca 400010, Romania. cmogosan@umfcluj.ro.
authors.forename: Cristina
authors.initials: C
authors.lastname: Mogoșan
authors.affiliation: Faculty of Pharmacy, Iuliu Hațieganu University of Medicine and Pharmacy, 8, V. Babes Street, Cluj-Napoca 400010, Romania. mocan.andrei@umfcluj.ro.
authors.forename: Andrei
authors.initials: A
authors.lastname: Mocan
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors; D002851:Chromatography, High Pressure Liquid; D004232:Diuretics / Q000737:chemistry / Q000494:pharmacology*; D005419:Flavonoids / Q000302:isolation & purification; D044949:Kaempferols / Q000302:isolation & purification; D008297:Male; D031861:Nigella damascena / Q000737:chemistry*; D031881:Nigella sativa / Q000737:chemistry*; D010851:Picrates / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D059808:Polyphenols / Q000302:isolation & purification*; D011188:Potassium / Q000652:urine; D011794:Quercetin / Q000031:analogs & derivatives / Q000302:isolation & purification; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds / Q000737:chemistry*; D012964:Sodium / Q000652:urine; D013045:Species Specificity; D014527:Uric Acid / Q000652:urine
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D004232:Diuretics; D005419:Flavonoids; D044949:Kaempferols; D010851:Picrates; D010936:Plant Extracts; D059808:Polyphenols; D014527:Uric Acid; C006552:kaempferol; C021304:hyperoside; D011794:Quercetin; D012964:Sodium; C004931:1,1-diphenyl-2-picrylhydrazyl; D011188:Potassium
keywords: N. damascena L.; Nigella sativa L.; antioxidants; diuretic; polyphenols; seeds