title: Anticancer activity of biostabilized selenium nanorods synthesized by Streptomyces bikiniensis strain Ess_amA-1.
abstract: Selenium is an important component of human diet and a number of studies have declared its chemopreventive and therapeutic properties against cancer. However, very limited studies have been conducted about the properties of selenium nanostructured materials in comparison to other well-studied selenospecies. Here, we have shown that the anticancer property of biostabilized selenium nanorods (SeNrs) synthesized by applying a novel strain Ess_amA-1 of Streptomyces bikiniensis. The strain was grown aerobically with selenium dioxide and produced stable SeNrs with average particle size of 17 nm. The optical, structural, morphological, elemental, and functional characterizations of the SeNrs were carried out using techniques such as UV-vis spectrophotometry, transmission electron microscopy, energy dispersive X-ray spectrometry, and Fourier transform infrared spectrophotometry, respectively. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay revealed that the biosynthesized SeNrs induces cell death of Hep-G2 and MCF-7 human cancer cells. The lethal dose (LD50%) of SeNrs on Hep-G2 and MCF-7 cells was recorded at 75.96 μg/mL and 61.86 μg/mL, respectively. It can be concluded that S. bikiniensis strain Ess_amA-1 could be used as renewable bioresources of biosynthesis of anticancer SeNrs. A hypothetical mechanism for anticancer activity of SeNrs is also proposed.
authors.affiliation: Department of Botany, Faculty of Science, University of Beni-Suef, Beni-Suef, Egypt.
authors.forename: Maged Sayed
authors.initials: MS
authors.lastname: Ahmad
authors.affiliation: Department of Botany, Faculty of Science, University of Beni-Suef, Beni-Suef, Egypt.
authors.forename: Manal Mohamed
authors.initials: MM
authors.lastname: Yasser
authors.affiliation: Department of Botany, Faculty of Science, University of Beni-Suef, Beni-Suef, Egypt ; Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Essam Nageh
authors.initials: EN
authors.lastname: Sholkamy
authors.affiliation: Department of Botany and Microbiology, College of Science, Minia University, El-Minia, Egypt ; Department of Biological Sciences, College of Science, King Faisal University, Saudi Arabia.
authors.forename: Ali Mohamed
authors.initials: AM
authors.lastname: Ali
authors.affiliation: Department of Botany and Microbiology, College of Science, Minia University, El-Minia, Egypt.
authors.forename: Magda Mohamed
authors.initials: MM
authors.lastname: Mehanni
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D056945:Hep G2 Cells / Q000187:drug effects; D006801:Humans; D061986:MCF-7 Cells / Q000187:drug effects; D046529:Microscopy, Electron, Transmission; D036103:Nanotechnology / Q000379:methods; D043942:Nanotubes / Q000737:chemistry*; D010316:Particle Size; D010802:Phylogeny; D012643:Selenium / Q000737:chemistry* / Q000494:pharmacology; D018036:Selenium Compounds; D013052:Spectrometry, X-Ray Emission; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D018036:Selenium Compounds; D012643:Selenium
keywords: Streptomyces; anticancer activity; biosynthesis; selenium nanorods