title: Discovery of a Novel Polymer for Human Pluripotent Stem Cell Expansion and Multilineage Differentiation.
abstract: A scalable and cost-effective synthetic polymer substrate that supports robust expansion and subsequent multilineage differentiation of human pluripotent stem cells (hPSCs) with defined commercial media is presented. This substrate can be applied to common cultureware and used off-the-shelf after long-term storage. Expansion and differentiation of hPSCs are performed entirely on the polymeric surface, enabling the clinical potential of hPSC-derived cells to be realized.
authors.affiliation: Laboratory of Biophysics and Surface Analysis, School of Pharmacy, University of Nottingham, Nottingham, NG7 2RD, UK.|Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, 02115, USA.
authors.forename: Adam D
authors.initials: AD
authors.lastname: Celiz
authors.affiliation: Wolfson Centre for Stem Cells, Tissue Engineering and Modelling, School of Medicine, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, NG7 2RD, UK.
authors.forename: James G W
authors.initials: JG
authors.lastname: Smith
authors.affiliation: Wolfson Centre for Stem Cells, Tissue Engineering and Modelling, School of Medicine, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, NG7 2RD, UK.|David H. Koch Institute for Integrative Cancer Research, Department of Chemical Engineering, Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
authors.forename: Asha K
authors.initials: AK
authors.lastname: Patel
authors.affiliation: Laboratory of Biophysics and Surface Analysis, School of Pharmacy, University of Nottingham, Nottingham, NG7 2RD, UK.
authors.forename: Andrew L
authors.initials: AL
authors.lastname: Hook
authors.affiliation: Wolfson Centre for Stem Cells, Tissue Engineering and Modelling, School of Medicine, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, NG7 2RD, UK.
authors.forename: Divya
authors.initials: D
authors.lastname: Rajamohan
authors.affiliation: Wolfson Centre for Stem Cells, Tissue Engineering and Modelling, School of Medicine, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, NG7 2RD, UK.
authors.forename: Vinoj T
authors.initials: VT
authors.lastname: George
authors.affiliation: Wolfson Centre for Stem Cells, Tissue Engineering and Modelling, School of Medicine, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, NG7 2RD, UK.
authors.forename: Luke
authors.initials: L
authors.lastname: Flatt
authors.affiliation: Wolfson Centre for Stem Cells, Tissue Engineering and Modelling, School of Medicine, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, NG7 2RD, UK.
authors.forename: Minal J
authors.initials: MJ
authors.lastname: Patel
authors.affiliation: CSIRO Manufacturing Flagship, 343 Royal Parade, Parkville, 3052, Australia.
authors.forename: Vidana C
authors.initials: VC
authors.lastname: Epa
authors.affiliation: Laboratory of Biophysics and Surface Analysis, School of Pharmacy, University of Nottingham, Nottingham, NG7 2RD, UK.
authors.forename: Taranjit
authors.initials: T
authors.lastname: Singh
authors.affiliation: David H. Koch Institute for Integrative Cancer Research, Department of Chemical Engineering, Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
authors.forename: Robert
authors.initials: R
authors.lastname: Langer
authors.affiliation: David H. Koch Institute for Integrative Cancer Research, Department of Chemical Engineering, Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
authors.forename: Daniel G
authors.initials: DG
authors.lastname: Anderson
authors.affiliation: Cardiff School of Biosciences, The Sir Martin Evans Building, Museum Avenue, Cardiff, CF10 3AX, UK.
authors.forename: Nicholas D
authors.initials: ND
authors.lastname: Allen
authors.affiliation: MRC Centre for Regenerative Medicine SCRM Building, The University of Edinburgh, Edinburgh BioQuarter, 5 Little France Drive, Edinburgh, EH16 4UU, UK.
authors.forename: David C
authors.initials: DC
authors.lastname: Hay
authors.affiliation: CSIRO Manufacturing Flagship, Bayview Avenue, Clayton, 3168, Australia.|Monash Institute of Pharmaceutical Sciences, 399 Royal Parade, Parkville, 3052, Australia.|Latrobe Institute for Molecular Science, Latrobe University, Bundoora, 3086, Australia.
authors.forename: David A
authors.initials: DA
authors.lastname: Winkler
authors.affiliation: Centre for Analytical Bioscience, School of Pharmacy, University of Nottingham, Nottingham, NG7 2RD, UK.
authors.forename: David A
authors.initials: DA
authors.lastname: Barrett
authors.affiliation: Laboratory of Biophysics and Surface Analysis, School of Pharmacy, University of Nottingham, Nottingham, NG7 2RD, UK.
authors.forename: Martyn C
authors.initials: MC
authors.lastname: Davies
authors.affiliation: Wolfson Centre for Stem Cells, Tissue Engineering and Modelling, School of Medicine, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, NG7 2RD, UK.
authors.forename: Lorraine E
authors.initials: LE
authors.lastname: Young
authors.affiliation: Wolfson Centre for Stem Cells, Tissue Engineering and Modelling, School of Medicine, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, NG7 2RD, UK.
authors.forename: Chris
authors.initials: C
authors.lastname: Denning
authors.affiliation: Laboratory of Biophysics and Surface Analysis, School of Pharmacy, University of Nottingham, Nottingham, NG7 2RD, UK.
authors.forename: Morgan R
authors.initials: MR
authors.lastname: Alexander
mesh_terms: D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000295:instrumentation*; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D019070:Cell Lineage; D003470:Culture Media; D005455:Fluorescent Antibody Technique; D057166:High-Throughput Screening Assays; D006801:Humans; D046228:Microarray Analysis; D039904:Pluripotent Stem Cells / Q000502:physiology*; D011108:Polymers
chemical_list: D003470:Culture Media; D011108:Polymers
keywords: high throughput; human pluripotent stem cells; materials discovery; polymer microarrays; stem cell differentiation