title: [Effect of Triptolide on expression of NMDAR1 and BSI-B4 binding sites in spinal dorsal horn and dorsal root ganglion in rats with adjuvant arthritis].
abstract: OBJECTIVE
  To study the analgesic effect of Triptolide(TP) in rats with adjuvant and the possible mechanism.
  
  
  METHODS
  Fifty healthy SD rats were randomly divided into normal control group (group A), model group (group B), and low(group C), middle (group D) and high(group E) dose TP treatment groups. Except the group A, each group of rats were reared by toe intradermal injection of 0. 1 mL Freund's complete adjuvant. After 14 days,rats in the C, D and E groups were taken different doses (0. 1 mg/kg group C, 0. 2mg/kg group D, and 0. 4 mg/kg group E) by intraperitoneal injection of TP for 9 days, and then thermal withdrawal latency and the expression of NMDAR1 and BSI-B4 binding sites in lumbar5 (L5) spinal dorsal horn and DRG were detected.
  
  
  RESULTS
  Thermal withdrawal latency of rats in group B was significantly lower than that of group A (P <0. 01), while those in group C, D and E were significantly higher than those in group B (P <0. 05 or P <0. 01). TP increased the thermal pain threshold by a quantity-effect relationship; NMDAR-1 and BSI-B4 binding sites expression levels were significantly increased in group B than those in group A (P <0. 01), while those in group C, D and E were lower than those in group B.
  
  
  CONCLUSIONS
  Analgesic effect of TP is related to reducing levels of expression of NMDAR1 and BSI-B4 binding sites in spinal dorsal horn and DRG in rats with adjuvant arthritis.
authors.forename: Yun-bo
authors.initials: YB
authors.lastname: Wei
authors.forename: Rong
authors.initials: R
authors.lastname: Lin
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Xu-dong
authors.initials: XD
authors.lastname: Zhang
authors.forename: Zhuo-hui
authors.initials: ZH
authors.lastname: Lu
authors.forename: Wen-wen
authors.initials: WW
authors.lastname: Jiang
authors.forename: Deng-ming
authors.initials: DM
authors.lastname: Wei
mesh_terms: D000700:Analgesics / Q000494:pharmacology*; D000818:Animals; D001169:Arthritis, Experimental / Q000188:drug therapy* / Q000378:metabolism; D001665:Binding Sites; D004224:Diterpenes / Q000494:pharmacology*; D004852:Epoxy Compounds / Q000494:pharmacology; D005620:Freund's Adjuvant; D005727:Ganglia, Spinal / Q000187:drug effects* / Q000378:metabolism; D007274:Injections, Intraperitoneal; D010146:Pain / Q000188:drug therapy*; D010147:Pain Measurement; D010616:Phenanthrenes / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D066148:Spinal Cord Dorsal Horn / Q000166:cytology
chemical_list: D000700:Analgesics; D004224:Diterpenes; D004852:Epoxy Compounds; D005620:Freund's Adjuvant; D010616:Phenanthrenes; D016194:Receptors, N-Methyl-D-Aspartate; C001899:triptolide