title: Dietary supplementation of Butyrivibrio fibrisolvens alters fatty acids of milk and rumen fluid in lactating goats.
abstract: BACKGROUND
  Conjugated linoleic acid (CLA) isomers have high health amelioration potential and hence it is of great interest to increase the CLA content in dairy products. The present study was conducted to investigate the effect of administration of high CLA producing Butyrivibrio fibrisolvens In-1 on fatty acid composition of milk and rumen fluid in lactating goats. Four groups (n = 5) of lactating goats were assigned the following treatments: Control (C) (basal diet); T1 (basal diet + linoleic acid source), T2 (basal diet + suspension of Butyrivibrio fibrisolvens In-1, 10(9) CFU head(-1)) and T3 (basal diet + linoleic acid source + suspension of Butyrivibrio fibrisolvens In-1, 10(9) CFU head(-1)).
  
  
  RESULTS
  Rumen liquor and milk samples were collected on days 0, 15, 30, 60 and 90 of the experiment and linoleic isomerase enzyme (LA-I) activity and fatty acid profiles were elucidated. Major effects of treatments were seen on day 30 of the experiment. Total CLA content of rumen fluid increased (P < 0.05) by 218.72, 182.26 and 304% whereas total saturated fatty acid (SFA) content was lowered (P < 0.05) by 6.1, 4.44 and 9.55% in T1, T2 and T3, respectively, as compared to control. Vaccenic acid in groups T2 and T3 increased (P < 0.05) by 66.67% and 105.7% as compared to control. In milk, total CLA increased by 2.03, 1.61 and 0.61 folds in T3, T2 and T1, respectively. Total monounsaturated fatty acid and polyunsaturated fatty acid content increased (P < 0.05) in group T3 by 14.15 and 37.44%, respectively.
  
  
  CONCLUSIONS
  Results of the present study indicated that administration of B. fibrisolvens In-1 along with a linoleic acid (LA) source is a useful strategy to alter the biohydrogenation pattern in the rumen that subsequently decreased SFA content while increased CLA and unsaturated fatty acids in ruminant's milk.
authors.affiliation: Dairy Cattle Nutrition Division, National Dairy Research Institute, Karnal-132001, Haryana, India.
authors.forename: Swati
authors.initials: S
authors.lastname: Shivani
authors.affiliation: Dairy Cattle Nutrition Division, National Dairy Research Institute, Karnal-132001, Haryana, India.
authors.forename: Anima
authors.initials: A
authors.lastname: Srivastava
authors.affiliation: Dairy Cattle Nutrition Division, National Dairy Research Institute, Karnal-132001, Haryana, India.
authors.forename: Umesh K
authors.initials: UK
authors.lastname: Shandilya
authors.affiliation: Dairy Cattle Nutrition Division, National Dairy Research Institute, Karnal-132001, Haryana, India.
authors.forename: Vishnu
authors.initials: V
authors.lastname: Kale
authors.affiliation: Dairy Cattle Nutrition Division, National Dairy Research Institute, Karnal-132001, Haryana, India.
authors.forename: Amrish K
authors.initials: AK
authors.lastname: Tyagi
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D001826:Body Fluids / Q000737:chemistry; D000070097:Butyrivibrio fibrisolvens / Q000378:metabolism*; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D006041:Goats / Q000502:physiology*; D007774:Lactation / Q000502:physiology*; D008892:Milk / Q000737:chemistry*; D012417:Rumen / Q000737:chemistry
chemical_list: D005227:Fatty Acids
keywords: Butyrivibrio fibrisolvens; conjugated linoleic acid; fatty acids; lactating goat