title: Osteoblast-derived microvesicles: A novel mechanism for communication between osteoblasts and osteoclasts.
abstract: The maintenance of bone homeostasis is largely dependent upon cellular communication between osteoclasts and osteoblasts. Microvesicles (MVs) have received a good deal of attention and are increasingly considered as mediators of intercellular communication due to their capacity to merge with and transfer a repertoire of bioactive molecular content (cargo) to recipient cells, triggering a variety of biologic responses. Here, we demonstrated that MVs shed from osteoblasts contain RANKL protein and can transfer it to osteoclast precursors through receptor ligand (RANKL-RANK), leading to stimulation of RANKL-RANK signaling to facilitate osteoclast formation. Such MV-mediated intercellular communication between osteoblasts and osteoclasts may represent a novel mechanism of bone modeling and remodeling. It may be worthwhile to further explore MVs as tools to modify the biological responses of bone cells or develop an alternative drug to treat bone diseases.
authors.affiliation: Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi, Jiangsu, China. Electronic address: denglili@jsinm.org.
authors.forename: Lili
authors.initials: L
authors.lastname: Deng
authors.affiliation: Wuxi Second People's Hospital of Nanjing Medical University, Wuxi, Jiangsu, China. Electronic address: ypwnag0301@126.com.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi, Jiangsu, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi, Jiangsu, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi, Jiangsu, China.
authors.forename: Yuedi
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Wuxi Second People's Hospital of Nanjing Medical University, Wuxi, Jiangsu, China.
authors.forename: Yuhai
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Jiangsu Provincial Taihu Rehabilitation Hospital, Wuxi, Jiangsu, China.
authors.forename: Zhenhai
authors.initials: Z
authors.lastname: Shen
authors.affiliation: Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi, Jiangsu, China. Electronic address: fuqiang@jsinm.org.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D016723:Bone Remodeling / Q000502:physiology*; D002450:Cell Communication / Q000502:physiology*; D002460:Cell Line; D055252:Cell-Derived Microparticles / Q000378:metabolism*; D005434:Flow Cytometry; D051379:Mice; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D010006:Osteoblasts / Q000378:metabolism*; D010010:Osteoclasts / Q000378:metabolism*; D053245:RANK Ligand / Q000378:metabolism
chemical_list: D053245:RANK Ligand
keywords: Cellular communication; Microvesicles; Osteoblast; Osteoclast; RANKL–RANK pathway