title: The complete mitochondrial genome of Gloydius intermedius (Squamata: Viperidae: Crotalinae) from China.
abstract: The mitochondrial genome sequence of Gloydius intermedius is analyzed and presented for the first time. The genome was 17, 226 bp in length and contained 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and 2 control region. The overall base composition was A (32.4%), C (28.8%), T (25.9%), and G (12.9%). The base compositions clearly presented the A-C skew, which was most obvious in the protein-coding genes. The extended termination-associated sequence domain, the central conserved domain and the conserved sequence block domain are defined in the mitochondrial genome control region of G. intermedius. Mitochondrial genome analyses based on MP, ML, NJ and Bayesian analyses yielded identical phylogenetic trees, indicating a close phylogenetic affinity of the 13 Crotalinae species. It appeared that no less than two major phyletic lineages were present in Crotalinae. The main clades within the Crotalinae supported are: A clade including the Protobothrops. A clade (G. brevicaudus, G. ussuriensis, G. intermedius, G. saxatilis) with the Ovophis appeared as the sister taxon to Protobothrops and was supported by bootstrap values of 88%. The four Gloydius species formed a paraphyletic group with the high bootstrap value (100 %) in all examinations.
authors.affiliation: a College of Life Science, Northeast Agricultural University , Harbin , P.R. China and.
authors.forename: Chunzhu
authors.initials: C
authors.lastname: Xu
authors.affiliation: a College of Life Science, Northeast Agricultural University , Harbin , P.R. China and.
authors.forename: Shuai
authors.initials: S
authors.lastname: Zhao
authors.affiliation: a College of Life Science, Northeast Agricultural University , Harbin , P.R. China and.
authors.forename: Cheng
authors.initials: C
authors.lastname: Li
authors.affiliation: b Dalai Lake Nature Reserve , Hailar , P.R. China.
authors.forename: Huashan
authors.initials: H
authors.lastname: Dou
mesh_terms: D000818:Animals; D001482:Base Composition; D000073562:Crotalinae / Q000145:classification* / Q000235:genetics*; D050259:Genes, Mitochondrial; D059646:Genome Size; D054629:Genome, Mitochondrial; D016366:Open Reading Frames; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D000073336:Whole Genome Sequencing
keywords: Control region; Gloydius intermedius; mitogenome; phylogenetic tree