title: A tyrosine-based motif in the HIV-1 envelope glycoprotein tail mediates cell-type- and Rab11-FIP1C-dependent incorporation into virions.
abstract: Lentiviruses such as HIV-1 encode envelope glycoproteins (Env) with long cytoplasmic tails (CTs) that include motifs mediating interactions with host-cell-trafficking factors. We demonstrated recently that Rab11-family interacting protein 1C (FIP1C) is required for CT-dependent incorporation of Env into HIV-1 particles. Here, we used viruses bearing targeted substitutions within CT to map the FIP1C-dependent incorporation of Env. We identified YW795 as a critical motif mediating cell-type-dependent Env incorporation. Disruption of YW795 reproduced the cell-type-dependent particle incorporation of Env that had previously been observed with large truncations of CT. A revertant virus bearing a single amino acid change near the C terminus of CT restored wild-type levels of Env incorporation, Gag-Env colocalization on the plasma membrane, and viral replication. These findings highlight the importance of YW795 in the cell-type-dependent incorporation of Env and support a model of HIV assembly in which FIP1C/RCP mediates Env trafficking to the particle assembly site.
authors.affiliation: Department of Pediatrics, Emory University and Children's Healthcare of Atlanta, Atlanta, Georgia 30322;
authors.forename: Mingli
authors.initials: M
authors.lastname: Qi
authors.affiliation: Department of Microbiology, Queen Mary Hospital, University of Hong Kong, Hong Kong Special Administrative Region, China;
authors.forename: Hin
authors.initials: H
authors.lastname: Chu
authors.affiliation: Department of Pediatrics, Emory University and Children's Healthcare of Atlanta, Atlanta, Georgia 30322;
authors.forename: Xuemin
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Pediatrics, Emory University and Children's Healthcare of Atlanta, Atlanta, Georgia 30322;
authors.forename: Junghwa
authors.initials: J
authors.lastname: Choi
authors.affiliation: Department of Pediatrics, Emory University and Children's Healthcare of Atlanta, Atlanta, Georgia 30322;
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Wen
authors.affiliation: Department of Pediatrics, Emory University and Children's Healthcare of Atlanta, Atlanta, Georgia 30322;
authors.forename: Jason
authors.initials: J
authors.lastname: Hammonds
authors.affiliation: Department of Pediatrics, Emory University and Children's Healthcare of Atlanta, Atlanta, Georgia 30322;
authors.forename: Lingmei
authors.initials: L
authors.lastname: Ding
authors.affiliation: Emory Vaccine Center, Yerkes National Primate Research Center, Emory University, Atlanta, GA 30322.
authors.forename: Eric
authors.initials: E
authors.lastname: Hunter
authors.affiliation: Department of Pediatrics, Emory University and Children's Healthcare of Atlanta, Atlanta, Georgia 30322; paul.spearman@emory.edu.
authors.forename: Paul
authors.initials: P
authors.lastname: Spearman
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000502:physiology*; D020816:Amino Acid Motifs; D019943:Amino Acid Substitution; D002460:Cell Line; D002462:Cell Membrane / Q000821:virology; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D015700:HIV Envelope Protein gp41 / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000502:physiology*; D016296:Mutagenesis; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013601:T-Lymphocytes / Q000821:virology; D014443:Tyrosine / Q000737:chemistry; D014771:Virion / Q000502:physiology; D019065:Virus Assembly; D014779:Virus Replication; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D015700:HIV Envelope Protein gp41; D008565:Membrane Proteins; C488966:RAB11FIP1 protein, human; D011993:Recombinant Fusion Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus; C514787:gp41 protein, Human immunodeficiency virus 1; D049452:Green Fluorescent Proteins; D014443:Tyrosine
keywords: FIP1C; HIV assembly; HIV envelope; Rab coupling protein; pseudotyping