title: Up-Regulation of MiR-300 Promotes Proliferation and Invasion of Osteosarcoma by Targeting BRD7.
abstract: Increasing reports suggest that deregulated microRNAs (miRNAs) might provide novel therapeutic targets for cancers. However, the expression and function of miR-300 in osteosarcoma is still unknown. In our study, we found that the expression of miR-300 was up-regulated in osteosarcoma tissues and cells compared with paired adjacent non-tumor bone tissues and osteoblastic cells using RT-qPCR. The enforced expression of miR-300 could promote cell proliferation, invasion and epithelial-mesenchymal transition (EMT). Moreover, we identified that bromodomain-containing protein 7 (BRD7), a new tumor suppressor gene, was a direct target of miR-300. Ectopic expression of BRD7 could significantly inhibit miR-300-promoted proliferation, invasion and EMT. Therefore, our results identify an important role for miR-300 in osteosarcoma through regulating BRD7 expression.
authors.affiliation: Department of Orthopaedic Surgery, The Affiliated No.2 Hospital of Harbin Medical University, Harbin, Heilongjiang Province, 150086, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Xue
authors.affiliation: Department of Orthopaedic Surgery, the Fifth Hospital of Harbin City, Harbin, Heilongjiang Province, 150040, China.
authors.forename: Jindong
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Physical diagnosis, The Affiliated Hospital of Harbin Institute of Technology, Harbin, Heilongjiang Province, 150001, China.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Niu
authors.affiliation: Department of Orthopaedic Surgery, The Affiliated No.2 Hospital of Harbin Medical University, Harbin, Heilongjiang Province, 150086, China.
authors.forename: Gang
authors.initials: G
authors.lastname: An
authors.affiliation: Department of Orthopaedic Surgery, The Affiliated No.2 Hospital of Harbin Medical University, Harbin, Heilongjiang Province, 150086, China.
authors.forename: Yashan
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Orthopaedic Surgery, The Tumor Hospital of Harbin Medical University, Harbin, Heilongjiang Province, 150086, China.
authors.forename: Linying
authors.initials: L
authors.lastname: Ni
mesh_terms: D000704:Analysis of Variance; D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000502:physiology*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology*; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006801:Humans; D008156:Luciferases; D035683:MicroRNAs / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000503:physiopathology*; D009841:Oligonucleotides / Q000235:genetics; D012516:Osteosarcoma / Q000503:physiopathology*; D060888:Real-Time Polymerase Chain Reaction; D000092468:Bromodomain Containing Proteins
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D008156:Luciferases; D035683:MicroRNAs; D009841:Oligonucleotides; C485694:BRD7 protein, human; D000092468:Bromodomain Containing Proteins; C000596512:MIRN300 microRNA, human