title: The skin migratory stage of the schistosomulum of Schistosoma mansoni has a surface showing greater permeability and activity in membrane internalisation than other forms of skin or mechanical schistosomula.
abstract: Skin schistosomula can be prepared by collecting them after isolated mouse skin have been penetrated by cercariae in vitro. The schistosomula can also migrate out of isolated mouse skin penetrated by cercariae in vitro and from mouse skin penetrated by cercariae in vivo. Schistosomula can also be produced from cercariae applied through a syringe or in a vortex. When certain surface properties of the different forms of schistosomula were compared, those migrating from mouse skin penetrated by cercariae in vivo or in vitro had greatly increased permeability to membrane impermeant molecules such as Lucifer yellow and high molecular weight dextrans. These migrating forms also possessed surfaces which showed greatly enhanced uptake into internal membrane vesicles of the dye FM 143, a marker for endocytosis. This greatly enhanced activity and permeability of the surfaces of tissue migrating schistosomula is likely to be of great importance in the adaptation to the new host.
authors.affiliation: Laboratory of schistosomiasis,Rene Rachou,Fiocuz,1715 Augusto de Lima,Belo Horizonte,30190-002 MG,Brazil.
authors.forename: Wander
authors.initials: W
authors.lastname: DE Jesus Jeremias
authors.affiliation: Depatarmento de Cirurgia,Faculdade de Medicina UFMG,Belo Horizonte,MG,Brazil.
authors.forename: Jose Renan
authors.initials: JR
authors.lastname: DA Cunha Melo
authors.affiliation: Laboratory of schistosomiasis,Rene Rachou,Fiocuz,1715 Augusto de Lima,Belo Horizonte,30190-002 MG,Brazil.
authors.forename: Elio Hideo
authors.initials: EH
authors.lastname: Baba
authors.affiliation: Laboratory of schistosomiasis,Rene Rachou,Fiocuz,1715 Augusto de Lima,Belo Horizonte,30190-002 MG,Brazil.
authors.forename: Paulo Marcos Zech
authors.initials: PM
authors.lastname: Coelho
authors.affiliation: Centre for Open Studies,Glasgow University,Glasgow,UK.
authors.forename: John Robert
authors.initials: JR
authors.lastname: Kusel
mesh_terms: D000818:Animals; D005456:Fluorescent Dyes; D007546:Isoquinolines / Q000737:chemistry; D051379:Mice; D009068:Movement; D010539:Permeability; D012550:Schistosoma mansoni / Q000502:physiology*; D012555:Schistosomiasis mansoni / Q000469:parasitology* / Q000473:pathology; D012867:Skin / Q000469:parasitology / Q000473:pathology
chemical_list: D005456:Fluorescent Dyes; D007546:Isoquinolines; C017475:lucifer yellow
keywords: Schistosomula; endocytosis; permeability; surface membrane