title: Intrusive growth of primary and secondary phloem fibres in hemp stem determines fibre-bundle formation and structure.
abstract: Plant fibres-cells with important mechanical functions and a widely used raw material-are usually identified in microscopic sections only after reaching a significant length or after developing a thickened cell wall. We characterized the early developmental stages of hemp (Cannabis sativa) stem phloem fibres, both primary (originating from the procambium) and secondary (originating in the cambium), when they still had only a primary cell wall. We gave a major emphasis to the role of intrusive elongation, the specific type of plant cell growth by which fibres commonly attain large cell length. We could identify primary phloem fibres at a distance of only 1.2-1.5 mm from the shoot apical meristem when they grew symplastically with the surrounding tissues. Half a millimeter further downwards along the stem, fibres began their intrusive elongation, which led to a sharp increase in fibre numbers visible within the stem cross-sections. The intrusive elongation of primary phloem fibres was completed within the several distal centimetres of the growing stem, before the onset of their secondary cell wall formation. The formation of secondary phloem fibres started long after the beginning of secondary xylem formation. Our data indicate that only a small portion of the fusiform cambial initials (<10 %) give rise directly or via their derivatives to secondary phloem fibres. The key determinant of final bundle structure, both for primary and secondary phloem fibres, is intrusive growth. Through bi-directional elongation, fibres join other fibres initiated individually in other stem levels, thus forming the bundles. Our results provide the specific developmental basis for further biochemical and molecular-genetic studies of phloem fibre development in hemp, but may be applied to many other species.
authors.affiliation: Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Centre, Russian Academy of Sciences, Kazan 420111, Russia.
authors.forename: Anastasia
authors.initials: A
authors.lastname: Snegireva
authors.affiliation: Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Centre, Russian Academy of Sciences, Kazan 420111, Russia.
authors.forename: Tatyana
authors.initials: T
authors.lastname: Chernova
authors.affiliation: Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Centre, Russian Academy of Sciences, Kazan 420111, Russia.
authors.forename: Marina
authors.initials: M
authors.lastname: Ageeva
authors.affiliation: Department of Biology and Environment, Faculty of Natural Sciences, University of Haifa - Oranim, Tivon 36006, Israel.
authors.forename: Simcha
authors.initials: S
authors.lastname: Lev-Yadun
authors.affiliation: Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Centre, Russian Academy of Sciences, Kazan 420111, Russia gorshkova@kibb.knc.ru.
authors.forename: Tatyana
authors.initials: T
authors.lastname: Gorshkova
keywords: Cambium; Cannabis sativa; hemp; intrusive growth; phloem fibre bundles; plant fibres