title: A combination of solid-phase extraction and dispersive solid-phase extraction effectively reduces the matrix interference in liquid chromatography-ultraviolet detection during pyraclostrobin analysis in perilla leaves.
abstract: Perilla leaves contain many interfering substances; thus, it is difficult to protect the analytes during identification and integration. Furthermore, increasing the amount of sample to lower the detection limit worsens the situation. To overcome this problem, we established a new method using a combination of solid-phase extraction and dispersive solid-phase extraction to analyze pyraclostrobin in perilla leaves by liquid chromatography with ultraviolet absorbance detection. The target compound was quantitated by external calibration with a good determination coefficient (R(2) = 0.997). The method was validated (in triplicate) with three fortification levels, and 79.06- 89.10% of the target compound was recovered with a relative standard deviation <4. The limits of detection and quantification were 0.0033 and 0.01 mg/kg, respectively. The method was successfully applied to field samples collected from two different areas at Gwangju and Muan. The decline in the resiudue concentrations was best ascribed to a first-order kinetic model with half-lives of 5.7 and 4.6 days. The variation between the patterns was attributed to humidity.
authors.affiliation: Biotechnology Research Institute, College of Agriculture and Life Sciences, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju, 500-757, Republic of Korea.
authors.forename: Waziha
authors.initials: W
authors.lastname: Farha
authors.affiliation: Biotechnology Research Institute, College of Agriculture and Life Sciences, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju, 500-757, Republic of Korea.
authors.forename: Md Musfiqur
authors.initials: MM
authors.lastname: Rahman
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul, 143-701, Republic of Korea.|Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, 12211, Giza, Egypt.
authors.forename: A M
authors.initials: AM
authors.lastname: Abd El-Aty
authors.affiliation: Biotechnology Research Institute, College of Agriculture and Life Sciences, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju, 500-757, Republic of Korea.
authors.forename: Da-I
authors.initials: DI
authors.lastname: Jung
authors.affiliation: Biotechnology Research Institute, College of Agriculture and Life Sciences, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju, 500-757, Republic of Korea.
authors.forename: Md Humayun
authors.initials: MH
authors.lastname: Kabir
authors.affiliation: Biotechnology Research Institute, College of Agriculture and Life Sciences, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju, 500-757, Republic of Korea.
authors.forename: Jeong-Heui
authors.initials: JH
authors.lastname: Choi
authors.affiliation: Biotechnology Research Institute, College of Agriculture and Life Sciences, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju, 500-757, Republic of Korea.
authors.forename: Sung-Woo
authors.initials: SW
authors.lastname: Kim
authors.affiliation: Biotechnology Research Institute, College of Agriculture and Life Sciences, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju, 500-757, Republic of Korea.
authors.forename: So Jeong
authors.initials: SJ
authors.lastname: Im
authors.affiliation: Biotechnology Research Institute, College of Agriculture and Life Sciences, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju, 500-757, Republic of Korea.
authors.forename: Young-Jun
authors.initials: YJ
authors.lastname: Lee
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul, 143-701, Republic of Korea.
authors.forename: Ho-Chul
authors.initials: HC
authors.lastname: Shin
authors.affiliation: Food Safety Division, Ministry of Food and Drug Safety, Cheongju, Republic of Korea.
authors.forename: Chan-Hyeok
authors.initials: CH
authors.lastname: Kwon
authors.affiliation: Food Safety Division, Ministry of Food and Drug Safety, Cheongju, Republic of Korea.
authors.forename: Young-Wook
authors.initials: YW
authors.lastname: Son
authors.affiliation: Food Safety Division, Ministry of Food and Drug Safety, Cheongju, Republic of Korea.
authors.forename: Kang-Bong
authors.initials: KB
authors.lastname: Lee
authors.affiliation: Biotechnology Research Institute, College of Agriculture and Life Sciences, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju, 500-757, Republic of Korea.
authors.forename: Jae-Han
authors.initials: JH
authors.lastname: Shim
mesh_terms: D002219:Carbamates / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D057230:Limit of Detection; D016014:Linear Models; D031343:Perilla / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D011720:Pyrazoles / Q000032:analysis*; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*; D000073739:Strobilurins
chemical_list: D002219:Carbamates; D011720:Pyrazoles; D000073739:Strobilurins; C513428:pyrachlostrobin
keywords: LC-UVD; SPE-d-SPE combination; interferences; perilla leaf; pyraclostrobin