title: Dietary Protein to Carbohydrate Ratio and Caloric Restriction: Comparing Metabolic Outcomes in Mice.
abstract: Both caloric restriction (CR) and low-protein, high-carbohydrate (LPHC) ad-libitum-fed diets increase lifespan and improve metabolic parameters such as insulin, glucose, and blood lipids. Severe CR, however, is unsustainable for most people; therefore, it is important to determine whether manipulating macronutrient ratios in ad-libitum-fed conditions can generate similar health outcomes. We present the results of a short-term (8 week) dietary manipulation on metabolic outcomes in mice. We compared three diets varying in protein to carbohydrate ratio under both CR and ad libitum conditions. Ad libitum LPHC diets delivered similar benefits to CR in terms of levels of insulin, glucose, lipids, and HOMA, despite increased energy intake. CR on LPHC diets did not provide additional benefits relative to ad libitum LPHC. We show that LPHC diets under ad-libitum-fed conditions generate the metabolic benefits of CR without a 40% reduction in total caloric intake.
authors.affiliation: Charles Perkins Centre, University of Sydney, Sydney, NSW 2006, Australia; Ageing and Alzheimers Institute and ANZAC Research Institute, Concord Hospital, University of Sydney, Sydney, NSW 2139, Australia; School of Biological Sciences, University of Sydney, Sydney, NSW 2006, Australia. Electronic address: samantha.solon-biet@sydney.edu.au.
authors.forename: Samantha M
authors.initials: SM
authors.lastname: Solon-Biet
authors.affiliation: Translational Gerontology Branch, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA.
authors.forename: Sarah J
authors.initials: SJ
authors.lastname: Mitchell
authors.affiliation: Charles Perkins Centre, University of Sydney, Sydney, NSW 2006, Australia; School of Biological Sciences, University of Sydney, Sydney, NSW 2006, Australia.
authors.forename: Sean C P
authors.initials: SC
authors.lastname: Coogan
authors.affiliation: Charles Perkins Centre, University of Sydney, Sydney, NSW 2006, Australia; Ageing and Alzheimers Institute and ANZAC Research Institute, Concord Hospital, University of Sydney, Sydney, NSW 2139, Australia.
authors.forename: Victoria C
authors.initials: VC
authors.lastname: Cogger
authors.affiliation: Ageing and Alzheimers Institute and ANZAC Research Institute, Concord Hospital, University of Sydney, Sydney, NSW 2139, Australia.
authors.forename: Rahul
authors.initials: R
authors.lastname: Gokarn
authors.affiliation: Charles Perkins Centre, University of Sydney, Sydney, NSW 2006, Australia; Ageing and Alzheimers Institute and ANZAC Research Institute, Concord Hospital, University of Sydney, Sydney, NSW 2139, Australia.
authors.forename: Aisling C
authors.initials: AC
authors.lastname: McMahon
authors.affiliation: Charles Perkins Centre, University of Sydney, Sydney, NSW 2006, Australia; School of Biological Sciences, University of Sydney, Sydney, NSW 2006, Australia; Faculty of Veterinary Science, University of Sydney, Sydney, NSW 2006, Australia.
authors.forename: David
authors.initials: D
authors.lastname: Raubenheimer
authors.affiliation: Translational Gerontology Branch, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA.
authors.forename: Rafael
authors.initials: R
authors.lastname: de Cabo
authors.affiliation: Charles Perkins Centre, University of Sydney, Sydney, NSW 2006, Australia; School of Biological Sciences, University of Sydney, Sydney, NSW 2006, Australia. Electronic address: stephen.simpson@sydney.edu.au.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Simpson
authors.affiliation: Charles Perkins Centre, University of Sydney, Sydney, NSW 2006, Australia; Ageing and Alzheimers Institute and ANZAC Research Institute, Concord Hospital, University of Sydney, Sydney, NSW 2139, Australia.
authors.forename: David G
authors.initials: DG
authors.lastname: Le Couteur
mesh_terms: D000818:Animals; D031204:Caloric Restriction; D004040:Dietary Carbohydrates / Q000008:administration & dosage / Q000378:metabolism*; D004044:Dietary Proteins / Q000008:administration & dosage / Q000378:metabolism*; D002149:Energy Intake; D005947:Glucose / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011897:Random Allocation
chemical_list: D004040:Dietary Carbohydrates; D004044:Dietary Proteins; D005947:Glucose