title: Zinc Supplementation in a Randomized Controlled Trial Decreased ZIP4 and ZIP8 mRNA Abundance in Peripheral Blood Mononuclear Cells of Adult Women.
abstract: Zinc plays an integral role in numerous cellular processes including regulation of gene expression. This randomized placebo-controlled trial in adult women evaluated the effects of 20 mg Zn for 23 days. The mRNA abundance of zinc transporters (ZnT1/ZIP3/ZIP4/ZIP8) and metallothionein (MT1) from peripheral blood mononuclear cells was determined by real-time quantitative polymerase chain reaction. In paired samples (n = 6-9), the ZIP4 (P = 0.036) and ZIP8 (P = 0.038) mRNA abundance decreased following zinc supplementation. ZnT1, ZIP3, and MT1 mRNA abundance did not change significantly. The mean ± standard deviation plasma zinc concentration (by inductively coupled plasma mass spectrometry) at baseline was 680 ± 110 μg/L for the zinc group (n = 24) and 741 ± 92 μg/L for the placebo group (n = 23). At endpoint, plasma zinc in the zinc group increased to 735 ± 80 μg/L (P < 0.01) while in the placebo group (717 ± 100 μg/L) it did not change significantly from baseline. The change in mRNA abundance highlights the importance of further investigating ZIP4 and ZIP8 mRNA abundance as potential zinc status biomarkers.
authors.affiliation: School of Nutrition, Food Science and Technology, Hawassa University, Hawassa, Ethiopia. ; Department of Nutritional Sciences, Oklahoma State University, Stillwater, OK, USA.
authors.forename: Alemtsehay
authors.initials: A
authors.lastname: Bogale
authors.affiliation: Department of Nutritional Sciences, Oklahoma State University, Stillwater, OK, USA.
authors.forename: Stephen L
authors.initials: SL
authors.lastname: Clarke
authors.affiliation: Department of Nutritional Sciences, Oklahoma State University, Stillwater, OK, USA.
authors.forename: Joanna
authors.initials: J
authors.lastname: Fiddler
authors.affiliation: School of Medicine, University of Colorado, Aurora, CO, USA.
authors.forename: K Michael
authors.initials: KM
authors.lastname: Hambidge
authors.affiliation: Department of Nutritional Sciences, Oklahoma State University, Stillwater, OK, USA.
authors.forename: Barbara J
authors.initials: BJ
authors.lastname: Stoecker
keywords: ZIP4; ZIP8; zinc; zinc supplementation