title: Real-Time Monitoring of Catheter-Related Biofilm Infection in Mice.
abstract: This study was done to establish a mouse model for catheter-related biofilm infection suitable to bioluminescence imaging (BLI). Biofilm formation of Pseudomonas aeruginosa (P. aeruginosa) Xen5 grown on catheter disks in vitro and in an implanted mouse model was real-time monitored during a 7-day study period using BLI. The numbers of integrated brightness (IB) and viable bacterial count (VBC) in the biofilm disks in vitro were highest at 24 h after inoculation; the IB of biofilm in vivo was increased until 24 h after implantation. A statistical correlation was observed between IB and VBC in vitro by linear regression analysis. The actual VBC value in vivo can be estimated accurately by IB without sacrifice. In addition, we monitored the change in white blood cells (WBCs) during infection. The number of WBCs on day 7 was significantly higher in the infection group than in the control group. This study indicates that BLI is a simple, fast, and sensitive method to measure catheter biofilm infection in mice.
authors.affiliation: Department of Clinical Pharmacology, PLA General Hospital, Beijing 100853, P.R. China.
authors.forename: Xu
authors.initials: X
authors.lastname: Liu
authors.affiliation: Traditional Chinese Medicine Pharmacy, PLA General Hospital, Beijing 100853, P.R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yin
authors.affiliation: Department of Pharmacy, the Second Artillery General Hospital, Beijing 100088, P.R. China.
authors.forename: Xianxing
authors.initials: X
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, P.R. China.
authors.forename: Yuanguo
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Medicine Clinical Research Center, PLA General Hospital, Beijing 100853, P.R. China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Medicine Clinical Research Center, PLA General Hospital, Beijing 100853, P.R. China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
mesh_terms: D000818:Animals; D058491:Bacterial Load / Q000379:methods*; D018441:Biofilms / Q000254:growth & development*; D055499:Catheter-Related Infections / Q000382:microbiology*; D057785:Catheters / Q000382:microbiology*; D004195:Disease Models, Animal; D007958:Leukocyte Count; D008163:Luminescent Measurements / Q000379:methods*; D051379:Mice; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000502:physiology*; D013997:Time Factors
keywords: biofilm; bioluminescence imaging; catheter-related infection; in vivo