title: S-resistin, a non secretable resistin isoform, impairs the insulin signalling pathway in 3T3-L1 adipocytes.
abstract: S-resistin is a non-secretable resistin spliced variant, which is expressed mainly in the white adipose tissue from Wistar rats. Previous results confirmed that 3T3-L1 cells expressing s-resistin (3T3-L1-s-res) showed an inflammatory response and exhibited a decrease in glucose transport, both basal and insulin-stimulated. Here we present evidences demonstrating for the first time that s-resistin, like resistin, blocks insulin signalling pathway by inhibiting insulin receptor, insulin receptor substrate 1, protein kinase B/Akt and the mammalian target of rapamycin phosphorylation, and increasing the suppressor of cytokine signalling 3 levels being the later probably due to augmented of leptin expression. Thus, our data suggest that s-resistin could act by a still unknown intracrine pathway as an intracellular sensor, regulating the adipocyte insulin sensitivity.
authors.affiliation: Área de Bioquímica. Facultad de Ciencias Ambientales y Bioquímica, Centro Regional de Investigaciones Biomédicas (CRIB), Universidad de Castilla-La Mancha, Av. Carlos III s/n, 45071, Toledo, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Rodríguez
authors.forename: Eduardo
authors.initials: E
authors.lastname: Moltó
authors.forename: Lidia
authors.initials: L
authors.lastname: Aguado
authors.forename: Nilda
authors.initials: N
authors.lastname: Gallardo
authors.forename: Antonio
authors.initials: A
authors.lastname: Andrés
authors.forename: Carmen
authors.initials: C
authors.lastname: Arribas
mesh_terms: D041721:3T3-L1 Cells; D050156:Adipogenesis; D000818:Animals; D016207:Cytokines / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors / Q000378:metabolism; D007328:Insulin / Q000502:physiology*; D007333:Insulin Resistance; D051379:Mice; D010766:Phosphorylation; D020033:Protein Isoforms / Q000502:physiology; D011499:Protein Processing, Post-Translational; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D052243:Resistin / Q000502:physiology*; D015398:Signal Transduction; D050826:Suppressor of Cytokine Signaling Proteins / Q000378:metabolism; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D015533:Transcriptional Activation
chemical_list: D016207:Cytokines; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors; C467200:Foxo1 protein, mouse; D007328:Insulin; D020033:Protein Isoforms; D052243:Resistin; C499623:Retn protein, mouse; D050826:Suppressor of Cytokine Signaling Proteins; D014157:Transcription Factors; C546843:mTOR protein, mouse; D051057:Proto-Oncogene Proteins c-akt; D058570:TOR Serine-Threonine Kinases