title: Traction microscopy to identify force modulation in subresolution adhesions.
abstract: We present a reconstruction algorithm that resolves cellular tractions in diffraction-limited nascent adhesions (NAs). The enabling method is the introduction of sparsity regularization to the solution of the inverse problem, which suppresses noise without underestimating traction magnitude. We show that NAs transmit a distinguishable amount of traction and that NA maturation depends on traction growth rate. A software package implementing this numerical approach is provided.
authors.affiliation: 1] Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, USA. [2] Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
authors.forename: Sangyoon J
authors.initials: SJ
authors.lastname: Han
authors.affiliation: Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: Youbean
authors.initials: Y
authors.lastname: Oak
authors.affiliation: Department of Physics, University of California, San Diego, La Jolla, California, USA.
authors.forename: Alex
authors.initials: A
authors.lastname: Groisman
authors.affiliation: 1] Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, USA. [2] Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
authors.forename: Gaudenz
authors.initials: G
authors.lastname: Danuser
mesh_terms: D002448:Cell Adhesion; D002478:Cells, Cultured; D008856:Microscopy, Fluorescence / Q000379:methods*; D012984:Software