title: Light-emitting diodes and their potential in callus growth, plantlet development and saponin accumulation during somatic embryogenesis ofPanax vietnamensisHaetGrushv.
abstract: In recent years, LED (light-emitting diode) has been the subject of research within the field of plant growth and development. However, there has been little discussion about using LEDin vitrocultures ofPanax vietnamensis, one of the important medicinal plants belonging to thePanaxgenus. This study examines the influence of various LED lamps on callus growth and plant formation ofP. vietnamensis. Results show significant differences in growth and development, as various light conditions were suitable for different stages. Callus of 70 mg in fresh weight cultured under yellow LEDs resulted in growth of 1197 mg in fresh weight and 91.7 mg of dry weight, within a period of three months. The most effective plant formation was obtained when embryogenic calli were cultured under the combination of 60% red LED and 40% blue LED with an average of 11.21 plantlets per explant; the shoot clump fresh weight and dry weight were of 1147 and 127 mg, respectively, and the average plant height was 3.1 cm. It was also shown that this light condition was the most efficient forP. vietnamensis in vitroplant growth and development. This study provided additional evidence regarding the influence of different LEDs on ginsenoside production applying high-performance liquid chromatography (HPLC) analysis with photo-diode array (PDA) detection at ultraviolet (UV) wavelength 203 nm. The highest MR2content was recorded when plants maintained under 20% red LED combined with 80% blue LED. However, the highest Rg1and Rb1content was found under fluorescent light. The results presented might provide new strategies using LEDs for adequate micropropagation protocols ofP. vietnamensis.
authors.affiliation: Tay Nguyen Institute for Scientific Research, Vietnam Academy of Science and Technology , Vietnam.
authors.forename: Duong Tan
authors.initials: DT
authors.lastname: Nhut
authors.affiliation: Tay Nguyen Institute for Scientific Research, Vietnam Academy of Science and Technology , Vietnam.
authors.forename: Nguyen Phuc
authors.initials: NP
authors.lastname: Huy
authors.affiliation: Tay Nguyen Institute for Scientific Research, Vietnam Academy of Science and Technology , Vietnam.
authors.forename: Ngo Thanh
authors.initials: NT
authors.lastname: Tai
authors.affiliation: Tay Nguyen Institute for Scientific Research, Vietnam Academy of Science and Technology , Vietnam.
authors.forename: Nguyen Ba
authors.initials: NB
authors.lastname: Nam
authors.affiliation: Tay Nguyen Institute for Scientific Research, Vietnam Academy of Science and Technology , Vietnam.
authors.forename: Vu Quoc
authors.initials: VQ
authors.lastname: Luan
authors.affiliation: Tay Nguyen Institute for Scientific Research, Vietnam Academy of Science and Technology , Vietnam.
authors.forename: Vu Thi
authors.initials: VT
authors.lastname: Hien
authors.affiliation: Tay Nguyen Institute for Scientific Research, Vietnam Academy of Science and Technology , Vietnam.
authors.forename: Hoang Thanh
authors.initials: HT
authors.lastname: Tung
authors.affiliation: Research Center of Ginseng and Medicinal Materials, National Institute of Medicinal Materials , Vietnam.
authors.forename: Bui The
authors.initials: BT
authors.lastname: Vinh
authors.affiliation: Research Center of Ginseng and Medicinal Materials, National Institute of Medicinal Materials , Vietnam.
authors.forename: Tran Cong
authors.initials: TC
authors.lastname: Luan
keywords: LEDs; Panax vietnamensis Ha et Grushv.; callus; spectrum