title: Impacts of drought stress on soluble carbohydrates and respiratory enzymes in fruit body ofAuricularia auricula.
abstract: In order to study the survival mechanisms to drought stress for fruit body ofAuricularia auricula, soluble carbohydrates and respiratory enzymes were investigated. Fruit bodies were exposed to sunlight and were naturally dehydrated. Samples were taken at different levels of water loss (0%, 10%, 30%, 50% and 70%) to measure the content of soluble sugars and polysaccharides. The activities of phosphoglucose isomerase (PGI), combined glucose-6-phosphate dehydrogenase (G-6-PDH) and 6-phosphogluconate dehydrogenase (6-PGDH), and malate dehydrogenase (MDH), were also determined. The results showed that with the increase in water loss, soluble sugars and MDH activity declined, whereas the activities of G-6-PDH and 6-PGDH increased. Soluble polysaccharides content and PGI activity decreased with water loss up to 30% and increased afterwards. These results suggested that the pentose phosphate pathway (PPP), as demonstrated by activities of G-6-PDH and 6-PGDH, could be one of the mechanisms for survival during drought stress in the fruit body ofA. auricula. Moreover, soluble polysaccharides may play a part in protecting the fruit body in further drought stress.
authors.affiliation: Department of Applied Microbiology, College of Resource and Environment, Northeast Agricultural University , Harbin, Heilongjiang , P.R. China ; Department of Biotechnology, College of Life Science and Technology, Mudanjiang Normal University , Mudanjiang, Heilongjiang , P.R. China.
authors.forename: Huai-Liang
authors.initials: HL
authors.lastname: Ma
authors.affiliation: Department of Applied Microbiology, College of Resource and Environment, Northeast Agricultural University , Harbin, Heilongjiang , P.R. China.
authors.forename: Xiu-Hong
authors.initials: XH
authors.lastname: Xu
authors.affiliation: Department of Applied Microbiology, College of Resource and Environment, Northeast Agricultural University , Harbin, Heilongjiang , P.R. China.
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Zhao
authors.affiliation: Department of Applied Microbiology, College of Resource and Environment, Northeast Agricultural University , Harbin, Heilongjiang , P.R. China.
authors.forename: Hua-Jing
authors.initials: HJ
authors.lastname: Liu
authors.affiliation: Department of Biotechnology, College of Life Science and Technology, Mudanjiang Normal University , Mudanjiang, Heilongjiang , P.R. China.
authors.forename: Huan
authors.initials: H
authors.lastname: Chen
keywords: Auricularia auricula; drought stress; fruit bodies; respiratory enzyme; soluble carbohydrates