title: [Effects of extracts of Dragon's blood on fibroblast proliferation and extracellular matrix hyaluronic acid].
abstract: OBJECTIVE
  To investigate the effects of Dragon' s blood extract on proliferation and secret extracellular matrix function of fibroblasts in vitro.
  
  
  METHODS
  Dragon' s blood was extracted by chloroform, acetoacetic ester, alcohol. Human fibroblast were cultured in vitro in media containing gradient dilutions of Dragon' s blood extracts (0.002, 0.02, 0.2, 2, 20 mg/ml) , which was followed by cell proliferation assessed with MTT assay on 0, 12, 24, 36, 48, 60, 72 h. Under the optimal concentration, the cell growth curves were drawn and the flow cytometry (FCM) was used to determine the changes of cell cycle. On 0, 12, 24, 36, 48, 60, 72 h, the concentration of hyaluronic acid in the supernatant of fibroblast culture was measured by radioimmunoassay.
  
  
  RESULTS
  0.2-2 mg/ml Dragon' s blood extracts enhanced the proliferation of fibroblasts in a dose-dependent manner. 2 mg/ml was the optimal dilution of Dragon's blood extract, and it increased the ratio of S cells in cell cycle [(25.80 ± 3.10)%] than control group [(7.50 ± 0.70)%, P < 0.01]. From 12 h to 72 h, in 2 mg/ml Dragon's blood group, concentration of Hyaluronic acid secreted by fibroblasts gradually increased, but were less than control (P < 0.01).
  
  
  CONCLUSIONS
  Dragon's blood acetoacetic ester extract improved the proliferation of cultured human fibroblasts in vitro, might be beneficial to promote wound healing.
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Rui
authors.initials: R
authors.lastname: Hui
authors.forename: Yongwu
authors.initials: Y
authors.lastname: Hu
authors.forename: Yan
authors.initials: Y
authors.lastname: Han
authors.forename: Shuzhong
authors.initials: S
authors.lastname: Guo
mesh_terms: D002453:Cell Cycle; D049109:Cell Proliferation / Q000187:drug effects*; D003470:Culture Media / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D005109:Extracellular Matrix; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D006820:Hyaluronic Acid / Q000032:analysis* / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D012116:Resins, Plant; D013997:Time Factors
chemical_list: D003470:Culture Media; D010936:Plant Extracts; D012116:Resins, Plant; D006820:Hyaluronic Acid; C525191:dragon's blood