title: Miniaturized technology for DNA typing: cassette PCR.
abstract: With the smaller size, low cost, and rapid testing capabilities, miniaturized lab-on-a-chip devices can change the way medical diagnostics are currently performed in the health-care system. We have demonstrated such a device that is self-contained, simple, disposable, and inexpensive. It is capable of performing DNA amplification on an inexpensive instrument suitable for near point of care settings. This technology will enable on the spot evaluation of patients in the clinic for faster medical decision-making and more informed therapeutic choices. Our device, a gel capillary cassette, termed cassette PCR, contains capillary reaction units each holding a defined primer set, with arrays of capillary reaction units for simultaneously detecting multiple targets. With the exception of the sample to be tested, each capillary reaction unit holds all the reagents needed for PCR in a desiccated form that can be stored at room temperature for up to 3 months and even longer in colder conditions. It relies on capillary forces for sample delivery of microliter volumes through capillaries, hence avoiding the need for pumps or valves. In the assembled cassette, the wax architecture supporting the capillaries melts during the PCR and acts as a vapor barrier as well as segregating capillaries with different primer sets. No other chip sealing techniques are required. Cassette PCR accepts raw samples such as urine, genital swabs, and blood. The cassette is made with off-the-shelf components and contains integrated positive and negative controls.
authors.affiliation: Department of Oncology, University of Alberta and Cross Cancer Institute, 11560 University Avenue, Edmonton, AB, Canada, T6G 1Z2, dmanage@ualberta.ca.
authors.forename: Dammika P
authors.initials: DP
authors.lastname: Manage
authors.forename: Linda M
authors.initials: LM
authors.lastname: Pilarski
mesh_terms: D004247:DNA / Q000235:genetics*; D016172:DNA Fingerprinting / Q000191:economics / Q000295:instrumentation* / Q000379:methods; D017931:DNA Primers / Q000235:genetics; D004867:Equipment Design; D006801:Humans; D056656:Lab-On-A-Chip Devices / Q000191:economics; D008904:Miniaturization / Q000295:instrumentation / Q000379:methods; D016133:Polymerase Chain Reaction / Q000191:economics / Q000295:instrumentation* / Q000379:methods
chemical_list: D017931:DNA Primers; D004247:DNA