title: Hijacked then lost in translation: the plight of the recombinant host cell in membrane protein structural biology projects.
abstract: Membrane protein structural biology is critically dependent upon the supply of high-quality protein. Over the last few years, the value of crystallising biochemically characterised, recombinant targets that incorporate stabilising mutations has been established. Nonetheless, obtaining sufficient yields of many recombinant membrane proteins is still a major challenge. Solutions are now emerging based on an improved understanding of recombinant host cells; as a 'cell factory' each cell is tasked with managing limited resources to simultaneously balance its own growth demands with those imposed by an expression plasmid. This review examines emerging insights into the role of translation and protein folding in defining high-yielding recombinant membrane protein production in a range of host cells.
authors.affiliation: School of Life & Health Sciences, Aston University, Aston Triangle, Birmingham B4 7ET, UK. Electronic address: r.m.bill@aston.ac.uk.
authors.forename: Roslyn M
authors.initials: RM
authors.lastname: Bill
authors.affiliation: Kent Fungal Group, School of Biosciences, University of Kent, Canterbury CT2 7NJ, UK.
authors.forename: Tobias
authors.initials: T
authors.lastname: von der Haar
mesh_terms: D000818:Animals; D003001:Cloning, Molecular / Q000379:methods; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics*; D008958:Models, Molecular; D014176:Protein Biosynthesis; D011487:Protein Conformation; D017510:Protein Folding; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D008565:Membrane Proteins; D011994:Recombinant Proteins