title: [Decreased expression of calcium-sensing receptor involved in the progression of diabetic cardiomyopathy].
abstract: OBJECTIVE
  To observe the dynamic expression of calcium-sensing receptor(CaSR) in myocardium of diabetic rats.
  
  
  METHODS
  Thirty male Wistar rats were randomly divided into 3 groups including control, diabetic-4 week and diabetic-8 week groups(n = 10). The type 2 diabetes mellitus models were established by intraperitoneal injection of streptozotocin (STZ, 30 mg/kg) after high-fat and high-sugar diet for one month. The cardiac morphology was observed by electron microscope. Western blot analyzed the expression of CaSR, phospholamban (PLN), a calcium handling regulator, and Ca+-ATPase(SERCA) in cardiac tissues.
  
  
  RESULTS
  Compared with control group, the expressions of CaSR and SERCA were decreased, while the expression of PLN was significantly increased in a time-dependent manner in diabetic groups. Meanwhile diabetic rats displayed abnormal cardiac structure.
  
  
  CONCLUSIONS
  These results indicate that the CaSR expression of myocardium is reduced in the progression of DCM, and its potential mechanism may be related to the imnaired intracellular calcium homeostasis.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Jia
authors.forename: Jian
authors.initials: J
authors.lastname: Sun
authors.forename: Hong-zhu
authors.initials: HZ
authors.lastname: Li
authors.forename: Hong-xia
authors.initials: HX
authors.lastname: Li
authors.forename: Xue
authors.initials: X
authors.lastname: Peng
authors.forename: Hong-jiang
authors.initials: HJ
authors.lastname: Shao
authors.forename: Jin-xia
authors.initials: JX
authors.lastname: Yang
authors.forename: Chang-qing
authors.initials: CQ
authors.lastname: Xu
authors.forename: Shu-zhi
authors.initials: SZ
authors.lastname: Bai
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000150:complications; D003924:Diabetes Mellitus, Type 2; D058065:Diabetic Cardiomyopathies / Q000378:metabolism* / Q000503:physiopathology; D018450:Disease Progression; D006321:Heart / Q000503:physiopathology; D008297:Male; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D044169:Receptors, Calcium-Sensing / Q000378:metabolism*; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000378:metabolism; D013311:Streptozocin; D000099211:Phospholamban
chemical_list: D002135:Calcium-Binding Proteins; D044169:Receptors, Calcium-Sensing; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D013311:Streptozocin; D000099211:Phospholamban