title: [Frontiers in Live Bone Imaging Researches. In vivo imaging of osteoblasts].
abstract: Osteoblasts are bone-forming cells and lately osteoblastic niche is getting a lot more attention as a candidate of hematopoietic niche. Although there have been many previous studies about osteoblasts, there are few studies about the dynamics of osteoblasts. Recent rapid advance of fluorescent imaging techniques enables us to observe cellular dynamics and there are some reports that osteoblasts were visualized in live bone by using intravital two-photon microscopy. Here we show past reports about live cell imaging of osteoblasts and our latest data of live cell imaging of osteoblasts in vivo and in vitro.
authors.affiliation: Department of Immunology and Cell Biology, Graduate School of Medicine and Frontier Biosciences, Osaka University, Japan./Japan Science and Technology Agency, CREST, Japan./Department of Orthopaedics, Graduate School of Medicine, Osaka University, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Furuya
authors.forename: Masaru
authors.initials: M
authors.lastname: Ishii
mesh_terms: D000818:Animals; D001862:Bone Resorption; D001842:Bone and Bones / Q000166:cytology* / Q000502:physiology; D002450:Cell Communication / Q000502:physiology; D002454:Cell Differentiation; D002478:Cells, Cultured; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton; D057054:Molecular Imaging / Q000379:methods* / Q000639:trends*; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure*; D010010:Osteoclasts / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology