title: Clinical significance of CUL4A in human prostate cancer.
abstract: Aberrant expression of the Cullin 4A (CUL4A) is found in many tumor types, but the functions and mechanism of CUL4A in prostate cancer (PCa) development and progression remain largely unknown. The aim of this study was to investigate the possible role of CUL4A in prostate tumorigenesis. Immunohistochemistry was used to examine CUL4A expression in human PCa tissues and BPH tissues. Cell proliferation was assessed by MTT, and migration and invasion were analyzed by Transwell and Matrigel assays after CUL4A knockdown in PCa in vitro. The results showed that CUL4A protein was overexpressed in 86.21 % of PCa tissues. CUL4A knockdown with siRNA in PCa cells decreased cell proliferation, migration, and invasion. Mechanistically, CUL4A could modulate the expression of P53 in PCa cells. Our results indicate that CUL4A overexpression play an oncogenic role in the pathogenesis of PCa, and CUL4A may be a potential therapeutic target for PCa.
authors.affiliation: Department of Urology, Affiliated Hospital of Weifang Medical University, No. 2428 Yuhe Road, Kuiwei District, Weifang, Shandong, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.affiliation: Department of Urology, Affiliated Hospital of Weifang Medical University, No. 2428 Yuhe Road, Kuiwei District, Weifang, Shandong, China. drzhzj6@163.com.
authors.forename: Zengjun
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Department of Urology, Affiliated Hospital of Weifang Medical University, No. 2428 Yuhe Road, Kuiwei District, Weifang, Shandong, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Lang
authors.affiliation: Department of Urology, Affiliated Hospital of Weifang Medical University, No. 2428 Yuhe Road, Kuiwei District, Weifang, Shandong, China.
authors.forename: Bao
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Urology, Affiliated Hospital of Weifang Medical University, No. 2428 Yuhe Road, Kuiwei District, Weifang, Shandong, China.
authors.forename: Dianjun
authors.initials: D
authors.lastname: Gao
mesh_terms: D063646:Carcinogenesis / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D044842:Cullin Proteins / Q000096:biosynthesis* / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: C114234:CUL4A protein, human; D044842:Cullin Proteins
keywords: CUL4A; P53; Proliferation; Prostate cancer; Targeted therapy