title: Activation of autophagy in rat brain cells following focal cerebral ischemia reperfusion through enhanced expression of Atg1/pULK and LC3.
abstract: The present study aimed to investigate the activation of Atg1/pULK, and LC3 in the cerebral cortex following focal cerebral ischemia reperfusion (CIR) injury, thereby examining its effect on autophagy in brain cells. Rat CIR models were established using the technique of middle cerebral artery occlusion. The neurological function score, TTC staining and the water content of brain tissue were used to evaluate the CIR model. Levels of autophagy in the brain cells were examined at different time‑points following CIR damage using electron microscopy. Immunohistochemistry and western blot analysis were also used for the qualitative and quantitative detection of levels of Atg1/pULK and LC3 in the cerebral cortex. Autophagy was observed in the early stage of CIR, and the expression of Atg1/pULK and LC3 were observed 1 h following CIR in the rats and reached peak expression levels after12 h, which following which the they gradually decreased. These results suggested Atg1/pULK and LC3 are key in the regulation of autophagy following CIR in the rat brain.
authors.affiliation: Department of Histology and Embryology, Liaoning Medical University, Jinzhou, Liaoning 121001, P.R. China.
authors.forename: Jingwei
authors.initials: J
authors.lastname: Yu
authors.affiliation: Key Laboratory of Molecular Cell Biology and New Drug Development, Liaoning Medical University, Jinzhou, Liaoning 121001, P.R. China.
authors.forename: Cuifen
authors.initials: C
authors.lastname: Bao
authors.affiliation: Department of Histology and Embryology, Liaoning Medical University, Jinzhou, Liaoning 121001, P.R. China.
authors.forename: Yanru
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Department of Histology and Embryology, Liaoning Medical University, Jinzhou, Liaoning 121001, P.R. China.
authors.forename: Xia
authors.initials: X
authors.lastname: Liu
mesh_terms: D000818:Animals; D001343:Autophagy; D000071189:Autophagy-Related Protein-1 Homolog; D001921:Brain / Q000166:cytology / Q000378:metabolism / Q000473:pathology*; D002545:Brain Ischemia / Q000378:metabolism / Q000473:pathology*; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D008297:Male; D008869:Microtubule-Associated Proteins / Q000032:analysis* / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology*
chemical_list: D000071189:Autophagy-Related Protein-1 Homolog; D047908:Intracellular Signaling Peptides and Proteins; D008869:Microtubule-Associated Proteins; C526109:Anxa3 protein, rat; C586255:ULK1 protein, rat