title: Off-target mutations are rare in Cas9-modified mice.
authors.affiliation: Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.
authors.forename: Vivek
authors.initials: V
authors.lastname: Iyer
authors.affiliation: Ministry of Education Key Laboratory of Model Animal for Disease Study, Model Animal Research Center of Nanjing University, Nanjing, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Shen
authors.affiliation: 1] Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK. [2] Cambridge-Suda Genome Research Center, Soochow University, Suzhou, China.
authors.forename: Wensheng
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.
authors.forename: Alex
authors.identifier: 0000000211680086
authors.initials: A
authors.lastname: Hodgkins
authors.affiliation: Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.
authors.forename: Thomas
authors.initials: T
authors.lastname: Keane
authors.affiliation: Ministry of Education Key Laboratory of Model Animal for Disease Study, Model Animal Research Center of Nanjing University, Nanjing, China.
authors.forename: Xingxu
authors.initials: X
authors.lastname: Huang
authors.affiliation: Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.
authors.forename: William C
authors.initials: WC
authors.lastname: Skarnes
mesh_terms: D000818:Animals; D001947:Breeding; D064130:CRISPR-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D064112:Clustered Regularly Interspaced Short Palindromic Repeats; D003850:Deoxyribonuclease I / Q000235:genetics / Q000378:metabolism; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D016678:Genome; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009154:Mutation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D064130:CRISPR-Associated Proteins; D012333:RNA, Messenger; D003850:Deoxyribonuclease I