title: Bioactive properties of commercialised pomegranate (Punica granatum) juice: antioxidant, antiproliferative and enzyme inhibiting activities.
abstract: Pomegranate juice and related products have long been used either in traditional medicine or as nutritional supplements claiming beneficial effects. Although there are several studies on this food plant, only a few studies have been performed with pomegranate juice or marketed products. The aim of this work is to evaluate the antioxidant effects of pomegranate juice on cellular models using hydrogen peroxide as an oxidizing agent or DPPH and superoxide radicals in cell free systems. The antiproliferative effects of the juice were measured on HeLa and PC-3 cells by the MTT assay and pharmacologically relevant enzymes (cyclooxygenases, xanthine oxidase, acetylcholinesterase and monoamine oxidase A) were selected for enzymatic inhibition assays. Pomegranate juice showed significant protective effects against hydrogen peroxide induced toxicity in the Artemia salina and HepG2 models; these effects may be attributed to radical scavenging properties of pomegranate as the juice was able to reduce DPPH and superoxide radicals. Moderate antiproliferative activities in HeLa and PC-3 cancer cells were observed. However, pomegranate juice was also able to inhibit COX-2 and MAO-A enzymes. This study reveals some mechanisms by which pomegranate juice may have interesting and beneficial effects in human health.
authors.affiliation: Department of Pharmacy, Faculty of Health Sciences, San Jorge University, Villanueva de Gállego (Zaragoza), Spain. ilopez@usj.es.
authors.forename: Francisco
authors.initials: F
authors.lastname: Les
authors.forename: Jose M
authors.initials: JM
authors.lastname: Prieto
authors.forename: Jose Miguel
authors.initials: JM
authors.lastname: Arbonés-Mainar
authors.forename: Marta Sofía
authors.initials: MS
authors.lastname: Valero
authors.forename: Víctor
authors.initials: V
authors.lastname: López
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D000975:Antioxidants / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D001156:Artemia / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D051546:Cyclooxygenase 2 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016861:Cyclooxygenase Inhibitors / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D058870:Food, Organic / Q000032:analysis / Q000191:economics; D000067030:Fruit and Vegetable Juices / Q000032:analysis* / Q000191:economics; D055951:Functional Food / Q000032:analysis* / Q000191:economics; D056945:Hep G2 Cells; D006801:Humans; D029561:Lythraceae / Q000737:chemistry*; D008995:Monoamine Oxidase / Q000737:chemistry / Q000378:metabolism; D008996:Monoamine Oxidase Inhibitors / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D016877:Oxidants / Q000037:antagonists & inhibitors / Q000633:toxicity; D018384:Oxidative Stress; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013030:Spain
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D016861:Cyclooxygenase Inhibitors; D004791:Enzyme Inhibitors; D008996:Monoamine Oxidase Inhibitors; D016877:Oxidants; D011994:Recombinant Proteins; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D008995:Monoamine Oxidase; C579458:monoamine oxidase A, human