title: Sustained prevention of biofilm formation on a novel silicone matrix suitable for medical devices.
abstract: Bacterial colonization and biofilm formation on medical devices constitute major challenges in clinical long-term use of e.g. catheters due to the risk of (re)infection of patients, which would result in additional use of antibiotics risking bacterial resistance development. The aim of the present project was to introduce a novel antibacterial approach involving an advanced composite material applicable for medical devices. The polymeric composites investigated consisted of a hydrogel network of cross-linked poly(2-hydroxyethyl methacrylate) (PHEMA) embedded in a poly(dimethylsiloxane) (PDMS) silicone elastomer produced using supercritical carbon dioxide (scCO2). In these materials, the hydrogel may contain an active pharmaceutical ingredient while the silicone elastomer provides the sufficient mechanical stability of the material. In these conceptual studies, the antimicrobial agent ciprofloxacin was loaded into the polymer matrix by a post-polymerization loading procedure. Sustained release of ciprofloxacin was demonstrated, and the release could be controlled by varying the hydrogel content in the range 13-38% (w/w) and by changing the concentration of ciprofloxacin during loading in the range of 1-20mg/mL. Devices containing 25% (w/w) hydrogel and loaded with ciprofloxacin displayed a strong antibacterial effect against Staphylococcus aureus bacterial colonization and subsequent biofilm formation on the device material was inhibited for 29days. In conclusion, the hydrogel/silicone composite represents a promising candidate material for medical devices that prevent bacterial colonization during long-term use.
authors.affiliation: Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, DK-2100 Copenhagen, Denmark.
authors.forename: Søren Langer
authors.initials: SL
authors.lastname: Steffensen
authors.affiliation: Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, DK-2100 Copenhagen, Denmark.
authors.forename: Merete Hedemark
authors.initials: MH
authors.lastname: Vestergaard
authors.affiliation: Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, DK-2100 Copenhagen, Denmark.
authors.forename: Minna
authors.initials: M
authors.lastname: Groenning
authors.affiliation: BioModics ApS, Gregersensvej 7, DK-2630 Taastrup, Denmark.
authors.forename: Martin
authors.initials: M
authors.lastname: Alm
authors.affiliation: Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, DK-2100 Copenhagen, Denmark.
authors.forename: Henrik
authors.initials: H
authors.lastname: Franzyk
authors.affiliation: Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, DK-2100 Copenhagen, Denmark. Electronic address: hanne.morck@sund.ku.dk.
authors.forename: Hanne Mørck
authors.initials: HM
authors.lastname: Nielsen
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D003692:Delayed-Action Preparations; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D065546:Drug Liberation; D004869:Equipment Safety; D004864:Equipment and Supplies / Q000382:microbiology* / Q000592:standards*; D008826:Microbial Sensitivity Tests; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry; D012826:Silicone Elastomers / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development
chemical_list: D000890:Anti-Infective Agents; D003692:Delayed-Action Preparations; D004129:Dimethylpolysiloxanes; D012826:Silicone Elastomers; D011102:Polyhydroxyethyl Methacrylate
keywords: Antibiotics; Biofilm; Drug delivery system; Hydrogel; Medical device; Poly(2-hydroxyethyl methacrylate); Sustained release