title: Different Astrocytic Activation between Adult Gekko japonicus and Rats during Wound Healing In Vitro.
abstract: Glial scar formation is a major obstacle to regeneration after spinal cord injury. Moreover, it has been shown that the astrocytic response to injury differs between species. Gekko japonicas is a type of reptile and it shows differential glial activation compared to that of rats. The purpose of the present study was to compare the proliferation and migration of astrocytes in the spinal cords of geckos and rats after injury in vitro. Spinal cord homogenate stimulation and scratch wound models were used to induce astrocytic activation in adult and embryonic rats, as well as in adult geckos. Our results indicated that astrocytes from the adult rat were likely activated by mechanical stimulation, even though they showed lower proliferation abilities than the astrocytes from the gecko under normal conditions. Furthermore, a transcriptome analysis revealed that the differentially expressed genes in astrocytes from adult rats and those from geckos were enriched in pathways involved in proliferation and the response to stimuli. This implies that intrinsic discrepancies in gene expression patterns might contribute to the differential activation of astrocytes between species.
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu, 226001, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu, 226001, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu, 226001, China.
authors.forename: Haijiao
authors.initials: H
authors.lastname: Chen
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu, 226001, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu, 226001, China.
authors.forename: Man
authors.initials: M
authors.lastname: Xu
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu, 226001, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Hua
authors.affiliation: Department of Histology and Embryology of Medical College, Nantong University, Nantong, Jiangsu, 226001, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Yao
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu, 226001, China.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu, 226001, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu, 226001, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Liu
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism* / Q000473:pathology; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000378:metabolism / Q000473:pathology; D005786:Gene Expression Regulation; D008116:Lizards; D051381:Rats; D017207:Rats, Sprague-Dawley; D013045:Species Specificity; D013119:Spinal Cord Injuries / Q000378:metabolism* / Q000473:pathology; D059467:Transcriptome; D014945:Wound Healing / Q000502:physiology*