title: [The effect of pH, aeration, and temperature on arachidonic acid synthesis by Mortierella alpina].
abstract: The effects of pH, aeration, and temperature on the growth of fungal strain Mortierella alpina LPM-301 and the synthesis of lipids and arachidonic acid in glycerol-containing medium were studied. Arachidonic acid production in the stationary growth phase was found to depend considerably on the pH value; it reached the optimum at pH 6.0 and was irreversibly inhibited at a pH of 3.0. The PO2 values in a range from 10 to 50% showed no marked effect on mycelium growth or the synthesis of lipids and arachidonic acid. The temperature optimum for arachidonic acid production was 20-22 degrees C. Under continuous cultivation, the amount of arachidonic acid reached 29.8% of lipids and 7.4% of biomass. The arachidonic acid yield from the glycerol consumed was 4.1% by mass and 8.8% by energy. It is suggested that arachidonic acid synthesis at an unfavorable pH and elevated temperatures was limited by the activity of A-12-desaturase and by the conversion of linoleic to arachidonic acid, respectively.
authors.forename: E G
authors.initials: EG
authors.lastname: Dedyukhina
authors.forename: T I
authors.initials: TI
authors.lastname: Chistyakova
authors.forename: A A
authors.initials: AA
authors.lastname: Mironov
authors.forename: S V
authors.initials: SV
authors.lastname: Kamzolova
authors.forename: I G
authors.initials: IG
authors.lastname: Minkevich
authors.forename: M B
authors.initials: MB
authors.lastname: Vainshtein
mesh_terms: D016718:Arachidonic Acid / Q000096:biosynthesis*; D018533:Biomass; D003470:Culture Media; D005990:Glycerol / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology / Q000379:methods; D008055:Lipids / Q000096:biosynthesis / Q000737:chemistry; D020094:Mortierella / Q000378:metabolism*; D013696:Temperature
chemical_list: D003470:Culture Media; D008055:Lipids; D016718:Arachidonic Acid; D005990:Glycerol