title: Organic Selenium Alleviated the Formation of Ethylene Glycol-Induced Calcium Oxalate Renal Calculi by Improving Osteopontin Expression and Antioxidant Capability in Dogs.
abstract: Twenty one-year-old local male dogs were randomly assigned into four groups (five dogs per group). The control and the ethylene glycol (EG) groups were fed basal diets without and with EG, and the EG+sodium selenite (EG+SS) and EG+selenium yeast (EG+SY) groups were fed basal diets with EG containing SS and SY, respectively. Blood, urine, and renal samples were taken after 18 weeks of feeding. The results showed that compared with the control group, the serum calcium levels and antioxidase activities significantly decreased in the EG group. Serum creatinine, urea nitrogen, and malondialdehyde (MDA) levels and urine calcium and oxalate levels significantly increased. Calcium oxalate crystal deposition and osteopontin (OPN) messenger RNA and protein expression in the renal tissues significantly increased. These changes above in the EG group were reversed within limits by adding selenium in the diets (both EG+SS and EG+SY groups). Further, compared with the EG+SS group, the EG+SY group showed better effects in decreasing the formation of EG-induced calcium oxalate renal calculi and OPN expression and improving antioxidant capability in dogs. It indicates that organic selenium has the potential value to alleviate the formation of EG-induced calcium oxalate renal calculi.
authors.affiliation: Institute of Nutritional and Metabolic Disorders in Domestic Animals and Fowls, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Yongwang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Nutritional and Metabolic Disorders in Domestic Animals and Fowls, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Haibin
authors.initials: H
authors.lastname: Xu
authors.affiliation: Institute of Nutritional and Metabolic Disorders in Domestic Animals and Fowls, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Wenting
authors.initials: W
authors.lastname: Zhong
authors.affiliation: Institute of Nutritional and Metabolic Disorders in Domestic Animals and Fowls, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Qingpeng
authors.initials: Q
authors.lastname: Shen
authors.affiliation: Institute of Nutritional and Metabolic Disorders in Domestic Animals and Fowls, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Tenghan
authors.initials: T
authors.lastname: Zhuang
authors.affiliation: Institute of Nutritional and Metabolic Disorders in Domestic Animals and Fowls, Nanjing Agricultural University, Nanjing, 210095, China. khhuang@njau.edu.cn.
authors.forename: Kehe
authors.initials: K
authors.lastname: Huang
mesh_terms: D000821:Animal Feed; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001806:Blood Urea Nitrogen; D002118:Calcium / Q000652:urine; D002129:Calcium Oxalate / Q000737:chemistry*; D003404:Creatinine / Q000097:blood; D004032:Diet; D004285:Dogs; D019855:Ethylene Glycol / Q000737:chemistry*; D007669:Kidney Calculi / Q000517:prevention & control*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D053495:Osteopontin / Q000378:metabolism*; D010070:Oxalates / Q000652:urine; D018384:Oxidative Stress; D012643:Selenium / Q000008:administration & dosage* / Q000737:chemistry; D016482:Urinalysis
chemical_list: D000975:Antioxidants; D010070:Oxalates; D053495:Osteopontin; D002129:Calcium Oxalate; D008315:Malondialdehyde; D003404:Creatinine; D019855:Ethylene Glycol; D012643:Selenium; D002118:Calcium
keywords: Antioxidation; Calcium oxalate; OPN; Renal calculi; Selenium