title: AMPK activation promotes lipid droplet dispersion on detyrosinated microtubules to increase mitochondrial fatty acid oxidation.
abstract: Lipid droplets (LDs) are intracellular organelles that provide fatty acids (FAs) to cellular processes including synthesis of membranes and production of metabolic energy. While known to move bidirectionally along microtubules (MTs), the role of LD motion and whether it facilitates interaction with other organelles are unclear. Here we show that during nutrient starvation, LDs and mitochondria relocate on detyrosinated MT from the cell centre to adopt a dispersed distribution. In the cell periphery, LD-mitochondria interactions increase and LDs efficiently supply FAs for mitochondrial beta-oxidation. This cellular adaptation requires the activation of the energy sensor AMPK, which in response to starvation simultaneously increases LD motion, reorganizes the network of detyrosinated MTs and activates mitochondria. In conclusion, we describe the existence of a specialized cellular network connecting the cellular energetic status and MT dynamics to coordinate the functioning of LDs and mitochondria during nutrient scarcity.
authors.affiliation: Cell Compartments and Signaling Group, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona 08036, Spain.
authors.forename: Albert
authors.initials: A
authors.lastname: Herms
authors.affiliation: Cell Compartments and Signaling Group, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona 08036, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Bosch
authors.affiliation: Department of Developmental and Cell Biology, UC Irvine, Irvine, California 92697, USA.
authors.forename: Babu J N
authors.initials: BJ
authors.lastname: Reddy
authors.affiliation: 1] The Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland 4072, Australia [2] Centre for Microscopy and Microanalysis, The University of Queensland, Brisbane, Queensland 4072, Australia.
authors.forename: Nicole L
authors.initials: NL
authors.lastname: Schieber
authors.affiliation: Cell Compartments and Signaling Group, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona 08036, Spain.
authors.forename: Alba
authors.initials: A
authors.lastname: Fajardo
authors.affiliation: Cell Compartments and Signaling Group, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona 08036, Spain.
authors.forename: Celia
authors.initials: C
authors.lastname: Rupérez
authors.affiliation: 1] Cell Compartments and Signaling Group, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona 08036, Spain [2] The Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland 4072, Australia [3] Centre for Microscopy and Microanalysis, The University of Queensland, Brisbane, Queensland 4072, Australia.
authors.forename: Andrea
authors.initials: A
authors.lastname: Fernández-Vidal
authors.affiliation: 1] The Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland 4072, Australia [2] Centre for Microscopy and Microanalysis, The University of Queensland, Brisbane, Queensland 4072, Australia.
authors.forename: Charles
authors.initials: C
authors.lastname: Ferguson
authors.affiliation: 1] Cell Compartments and Signaling Group, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona 08036, Spain [2] Departament de Biologia Cellular, Immunologia i Neurociències, Facultat de Medicina, Universitat de Barcelona, Barcelona 08036, Spain.
authors.forename: Carles
authors.initials: C
authors.lastname: Rentero
authors.affiliation: 1] Cell Compartments and Signaling Group, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona 08036, Spain [2] Departament de Biologia Cellular, Immunologia i Neurociències, Facultat de Medicina, Universitat de Barcelona, Barcelona 08036, Spain.
authors.forename: Francesc
authors.initials: F
authors.lastname: Tebar
authors.affiliation: 1] Cell Compartments and Signaling Group, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona 08036, Spain [2] Departament de Biologia Cellular, Immunologia i Neurociències, Facultat de Medicina, Universitat de Barcelona, Barcelona 08036, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Enrich
authors.affiliation: 1] The Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland 4072, Australia [2] Centre for Microscopy and Microanalysis, The University of Queensland, Brisbane, Queensland 4072, Australia.
authors.forename: Robert G
authors.identifier: 0000000274945248
authors.initials: RG
authors.lastname: Parton
authors.affiliation: Department of Developmental and Cell Biology, UC Irvine, Irvine, California 92697, USA.
authors.forename: Steven P
authors.initials: SP
authors.lastname: Gross
authors.affiliation: 1] Cell Compartments and Signaling Group, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona 08036, Spain [2] Departament de Biologia Cellular, Immunologia i Neurociències, Facultat de Medicina, Universitat de Barcelona, Barcelona 08036, Spain [3] Institució Catalana de Recerca i Estudis Avançats (ICREA), Barcelona 08010, Spain.
authors.forename: Albert
authors.identifier: 0000000217501085
authors.initials: A
authors.lastname: Pol
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D005227:Fatty Acids / Q000378:metabolism*; D066292:Lipid Droplets / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism*; D010084:Oxidation-Reduction; D014443:Tyrosine / Q000378:metabolism; D014709:Vero Cells
chemical_list: D005227:Fatty Acids; D014443:Tyrosine; D055372:AMP-Activated Protein Kinases