title: Lack of Artemisinin Resistance in Plasmodium falciparum in Uganda Based on Parasitological and Molecular Assays.
abstract: We evaluated markers of artemisinin resistance in Plasmodium falciparum isolated in Kampala in 2014. By standard in vitro assays, all isolates were highly sensitive to dihydroartemisinin (DHA). By the ring-stage survival assay, after a 6-h DHA pulse, parasitemia was undetectable in 40 of 43 cultures at 72 h. Two of 53 isolates had nonsynonymous K13-propeller gene polymorphisms but did not have the mutations associated with resistance in Asia. Thus, we did not see evidence for artemisinin resistance in Uganda.
authors.affiliation: Department of Natural Sciences and Mathematics, Dominican University of California, San Rafael, California, USA roland.cooper@dominican.edu.
authors.forename: Roland A
authors.initials: RA
authors.lastname: Cooper
authors.affiliation: Department of Medicine, University of California, San Francisco, California, USA.
authors.forename: Melissa D
authors.initials: MD
authors.lastname: Conrad
authors.affiliation: Department of Integrative Biology, University of California, Berkeley, California, USA.
authors.forename: Quentin D
authors.initials: QD
authors.lastname: Watson
authors.affiliation: Department of Natural Sciences and Mathematics, Dominican University of California, San Rafael, California, USA.
authors.forename: Stephanie J
authors.initials: SJ
authors.lastname: Huezo
authors.affiliation: Infectious Diseases Research Collaboration, Kampala, Uganda.
authors.forename: Harriet
authors.initials: H
authors.lastname: Ninsiima
authors.affiliation: Infectious Diseases Research Collaboration, Kampala, Uganda.
authors.forename: Patrick
authors.initials: P
authors.lastname: Tumwebaze
authors.affiliation: Infectious Diseases Research Collaboration, Kampala, Uganda.
authors.forename: Samuel L
authors.initials: SL
authors.lastname: Nsobya
authors.affiliation: Department of Medicine, University of California, San Francisco, California, USA.
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Rosenthal
mesh_terms: D000962:Antimalarials / Q000494:pharmacology*; D037621:Artemisinins / Q000494:pharmacology*; D004351:Drug Resistance; D006801:Humans; D016778:Malaria, Falciparum / Q000188:drug therapy* / Q000469:parasitology; D008969:Molecular Sequence Data; D018512:Parasitemia / Q000188:drug therapy*; D021261:Parasitic Sensitivity Tests; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D015800:Protozoan Proteins / Q000235:genetics; D014454:Uganda
chemical_list: D000962:Antimalarials; D037621:Artemisinins; D015800:Protozoan Proteins; C039060:artenimol