title: [Frontiers in Live Bone Imaging Researches. Two-Photon Excitation Microscopy, principles and technologies].
abstract: The "two photon absorption" phenomenon had been predicted by the American Physicist, Maria Ghöppert-Mayer in 1931. Denk and Webb group had proved it in 1990 and the first product had been launched in the market in 1996. But ever since the product became available, the number of users are not increased. Moreover, the system had been too difficult to use and the system sometimes stay not working in labs. But recently, the new easier-to-use products are released and the ultra short pulse IR laser became stable. And its applications are extending from neuro-science to oncology or immunology fields. Due to these reasons, the shipment of multi-photon microscope in Japan in 2013 is approximately 40 units which is 3 times bigger than in 2010. In this paper, I would like to discuss the principles of two-photon microscopy and some of the new technologies for the higher signal capture efficiency.
authors.affiliation: Application Engineering Section, Marketing Department, Microscope Solution Business Unit, Nikon corporation, Japan.
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Oikawa
mesh_terms: D000818:Animals; D007089:Image Enhancement / Q000295:instrumentation / Q000379:methods; D007564:Japan / Q000453:epidemiology; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation / Q000379:methods / Q000706:statistics & numerical data / Q000639:trends; D057054:Molecular Imaging / Q000295:instrumentation / Q000379:methods*; D009686:Nuclear Physics; D017785:Photons