title: Generation of a tamoxifen inducible Tnnt2MerCreMer knock-in mouse model for cardiac studies.
abstract: Tnnt2, encoding thin-filament sarcomeric protein cardiac troponin T, plays critical roles in heart development and function in mammals. To develop an inducible genetic deletion strategy in myocardial cells, we generated a new Tnnt2:MerCreMer (Tnnt2(MerCreMer/+)) knock-in mouse. Rosa26 reporter lines were used to examine the specificity and efficiency of the inducible Cre recombinase. We found that Cre was specifically and robustly expressed in the cardiomyocytes at embryonic and adult stages following tamoxifen induction. The knock-in allele on Tnnt2 locus does not impact cardiac function. These results suggest that this new Tnnt2(MerCreMer/+) mouse could be applied towards the temporal genetic deletion of genes of interests in cardiomyocytes with Cre-LoxP technology. The Tnnt2(MerCreMer/+) mouse model also provides a useful tool to trace myocardial lineage during development and repair after cardiac injury.
authors.affiliation: Department of Developmental and Regenerative Biology, The Black Family Stem Cell Institute, and the Mindich Child Health and Development Institute, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, New York, New York.
authors.forename: Jianyun
authors.initials: J
authors.lastname: Yan
authors.affiliation: Department of Developmental and Regenerative Biology, The Black Family Stem Cell Institute, and the Mindich Child Health and Development Institute, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, New York, New York.
authors.forename: Nishat
authors.initials: N
authors.lastname: Sultana
authors.affiliation: Department of Developmental and Regenerative Biology, The Black Family Stem Cell Institute, and the Mindich Child Health and Development Institute, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, New York, New York.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Leon H. Charney Division of Cardiology, New York University School of Medicine, 522 First Avenue, New York, New York.
authors.forename: David S
authors.initials: DS
authors.lastname: Park
authors.affiliation: Leon H. Charney Division of Cardiology, New York University School of Medicine, 522 First Avenue, New York, New York.
authors.forename: Akshay
authors.initials: A
authors.lastname: Shekhar
authors.affiliation: Department of Developmental and Regenerative Biology, The Black Family Stem Cell Institute, and the Mindich Child Health and Development Institute, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, New York, New York.
authors.forename: Jun
authors.initials: J
authors.lastname: Hu
authors.affiliation: Leon H. Charney Division of Cardiology, New York University School of Medicine, 522 First Avenue, New York, New York.
authors.forename: Lei
authors.initials: L
authors.lastname: Bu
authors.affiliation: Department of Developmental and Regenerative Biology, The Black Family Stem Cell Institute, and the Mindich Child Health and Development Institute, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, New York, New York.
authors.forename: Chen-Leng
authors.initials: CL
authors.lastname: Cai
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D018931:Antineoplastic Agents, Hormonal / Q000494:pharmacology; D004622:Embryo, Mammalian / Q000196:embryology / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D006321:Heart / Q000196:embryology / Q000254:growth & development / Q000502:physiology; D007150:Immunohistochemistry; D019426:Integrases / Q000235:genetics / Q000378:metabolism; D008297:Male; D008822:Mice, Transgenic; D023421:Models, Animal; D009130:Muscle, Smooth / Q000737:chemistry; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000378:metabolism; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D022661:RNA, Untranslated / Q000235:genetics; D013629:Tamoxifen / Q000494:pharmacology*; D013997:Time Factors; D020107:Troponin T / Q000235:genetics* / Q000378:metabolism; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D000199:Actins; D018931:Antineoplastic Agents, Hormonal; C110853:Gt(ROSA)26Sor non-coding RNA, mouse; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D022661:RNA, Untranslated; D020107:Troponin T; D013629:Tamoxifen; C045073:Cre recombinase; D019426:Integrases; D001616:beta-Galactosidase
keywords: MerCreMer; Tnnt2; cardiomyocyte; heart; tamoxifen