title: Ridge augmentation using recombinant human fibroblast growth factor-2 with biodegradable gelatin sponges incorporating β-tricalcium phosphate: a preclinical study in dogs.
abstract: OBJECTIVE
  Fibroblast growth factor-2 (FGF-2) regulates the proliferation and differentiation of osteogenic cells, resulting in the promotion of bone formation. Biodegradable gelatin sponges incorporating β-tricalcium phosphate (β-TCP) have been reported as a scaffold, which has the ability to control growth factor release, offering sufficient mechanical strength and efficient migration of mesenchymal cells. In this study, we evaluated the effects of the combined use of recombinant human FGF-2 (rhFGF-2) and gelatin/β-TCP sponge on ridge augmentation in dogs.
  
  
  METHODS
  Six male beagle dogs were used in this study. Twelve wk after tooth extraction, bilateral 10 × 5 mm (width × depth) saddle-type defects were created 3 mm apart from the mesial side of the maxillary canine. At the experimental sites, the defects were filled with gelatin/β-TCP sponge infiltrated with 0.3% rhFGF-2, whereas gelatin/β-TCP sponge infiltrated with saline was applied to the control sites. Eight wk after surgery, qualitative and quantitative analyses were performed.
  
  
  RESULTS
  There were no signs of clinical inflammation at 8 wk after surgery. Histometric measurements revealed that new bone height at the experimental sites (2.98 ± 0.65 mm) was significantly greater than that at the control sites (1.56 ± 0.66 mm; p = 0.004). The total tissue height was greater at the experimental sites (6.62 ± 0.66 mm) than that at the control sites (5.95 ± 0.74 mm), although there was no statistical significant difference (p = 0.051). Cast model measurements revealed that the residual defect height at the experimental sites (2.31 ± 0.50 mm) was significantly smaller than that at the control sites (3.51 ± 0.78 mm; p = 0.012).
  
  
  CONCLUSIONS
  The combined use of rhFGF-2 and gelatin/β-TCP sponge promotes ridge augmentation in canine saddle-type bone defects.
authors.affiliation: Department of Periodontology, Graduate School of Medical and Dental Science, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Hoshi
authors.affiliation: Department of Periodontology, Graduate School of Medical and Dental Science, Tokyo Medical and Dental University, Tokyo, Japan.|Division of Periodontology, Department of Oral Science, Graduate School of Dentistry, Kanagawa Dental University, Kanagawa, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Akizuki
authors.affiliation: Department of Periodontology, Graduate School of Medical and Dental Science, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Matsuura
authors.affiliation: Department of Periodontology, Graduate School of Medical and Dental Science, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ikawa
authors.affiliation: Department of Educational Media Development, Institute for Library and Media Information Technology, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Kinoshita
authors.affiliation: Oral Diagnosis and General Dentistry, University Hospital of Dentistry, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Oda
authors.affiliation: Department of Biomaterials, Kyoto University, Kyoto, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Tabata
authors.affiliation: Department of Biomaterials, Kyoto University, Kyoto, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Matsui
authors.affiliation: Department of Periodontology, Graduate School of Medical and Dental Science, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Izumi
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D002130:Calcium Phosphates; D004285:Dogs; D016222:Fibroblast Growth Factor 2 / Q000627:therapeutic use*; D005780:Gelatin; D006801:Humans; D008297:Male; D010012:Osteogenesis
chemical_list: D002130:Calcium Phosphates; D016222:Fibroblast Growth Factor 2; D005780:Gelatin
keywords: alveolar ridge augmentation; beta-tricalcium phosphate; fibroblast growth factor-2; gelatin