title: Determination of Miglitol by Column-Switching Ion-Pair HPLC with Tris(2,2'-bipyridine)ruthenium(II)-Electrogenerated Chemiluminescence Detection.
abstract: We have developed a highly sensitive, simple method for the quantitative determination of miglitol in standard serum samples using column-switching ion-pair HPLC with tris(2,2'-bipyridine)ruthenium(II)-electrogenerated chemiluminescence detection. The serum samples were directly injected into a column-switching HPLC system with a Shim-pack MAYI-SCX precolumn to remove the serum matrix. Chromatographic separation of miglitol was achieved on a TSKgel ODS 100-V column using a mobile phase containing sodium 1-octanesulfonate as an ion-pair reagent. The detection and quantification limits of miglitol were 3 and 10 ng/mL, respectively. The calibration curve for miglitol in the serum samples showed good linearity (r(2)=0.9997) in the range of 10-2500 ng/mL. The recovery rate of miglitol from the serum samples was more than 94% as calculated from blank serum samples spiked with miglitol 50, 100, 500, 1000, and 2000 ng/mL. Therefore, this method can be applied to routine therapeutic monitoring of miglitol in serum samples.
authors.affiliation: College of Industrial Technology, Nihon University.
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Asamoto
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Nobushi
authors.forename: Takahiko
authors.initials: T
authors.lastname: Oi
authors.forename: Kazuo
authors.initials: K
authors.lastname: Uchikura
mesh_terms: D017485:1-Deoxynojirimycin / Q000031:analogs & derivatives* / Q000097:blood; D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000737:chemistry; D017738:Alkanesulfonic Acids / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D056831:Coordination Complexes; D004867:Equipment Design; D006801:Humans; D007004:Hypoglycemic Agents / Q000097:blood*; D057230:Limit of Detection; D049449:Luminescence; D049408:Luminescent Agents / Q000737:chemistry*; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D017975:Ruthenium Compounds
chemical_list: D017485:1-Deoxynojirimycin; D015082:2,2'-Dipyridyl; D017738:Alkanesulfonic Acids; D056831:Coordination Complexes; D007004:Hypoglycemic Agents; D049408:Luminescent Agents; C547232:tris(2,2-bipyridine)-ruthenium(II); D017975:Ruthenium Compounds; C045621:miglitol; C042005:1-octanesulfonic acid