title: Virulence factors genes of Staphylococcus spp. isolated from caprine subclinical mastitis.
abstract: The aim of this study was to investigate genes involved in adhesion expression, biofilm formation, and enterotoxin production in isolates of Staphylococcus spp. from goats with subclinical mastitis and associate these results with the staphylococcal species. One hundred and twenty-four isolates were identified and polymerase chain reaction (PCR) was performed to detect the following genes: cna, ebpS, eno, fib, fnbA, fnbB, bap, sea, seb, sec, sed and see. The most commonly Staphylococcus species included S. epidermidis, S. lugdunensis, S. chromogenes, S. capitis ss capitis and S. intermedius. With the exception of fnbB, the genes were detected in different frequencies of occurrence in 86.3% of the Staphylococcus spp. isolates. Eno (73.2%) and bap (94.8%) were more frequently detected in coagulase-negative staphylococci (CNS); ebpS (76%), fib (90.9%) and fnbA (87%) were the most frequent genes in coagulase-positive staphylococci (CPS). Regarding enterotoxins, genes sed (28.2%) and see (24.2%) had a higher frequency of occurrence; sec gene was more frequently detected in CPS (58.8%). There was no association between the presence of the genes and the Staphylococcus species. Different virulence factors genes can be detected in caprine subclinical mastitis caused by CNS and CPS. The knowledge of the occurrence of these virulence factors is important for the development of effective control and prevention measures of subclinical mastitis caused by CNS and CPS in goats.
authors.affiliation: Departamento de Medicina Veterinária Preventiva e Saúde Animal (VPS), Faculdade de Medicina Veterinária e Zootecnia (FMVZ), Universidade de São Paulo (USP), Av. Prof. Dr. Orlando Marques de Paiva, 87, São Paulo, SP, Brazil. Electronic address: sandrasalaberry@yahoo.com.br.
authors.forename: Sandra Renata Sampaio
authors.initials: SR
authors.lastname: Salaberry
authors.affiliation: Departamento de Medicina Veterinária Preventiva e Saúde Animal (VPS), Faculdade de Medicina Veterinária e Zootecnia (FMVZ), Universidade de São Paulo (USP), Av. Prof. Dr. Orlando Marques de Paiva, 87, São Paulo, SP, Brazil.
authors.forename: André Becker Simões
authors.initials: AB
authors.lastname: Saidenberg
authors.affiliation: Departamento de Medicina Veterinária Preventiva e Saúde Animal (VPS), Faculdade de Medicina Veterinária e Zootecnia (FMVZ), Universidade de São Paulo (USP), Av. Prof. Dr. Orlando Marques de Paiva, 87, São Paulo, SP, Brazil.
authors.forename: Eveline
authors.initials: E
authors.lastname: Zuniga
authors.affiliation: Departamento de Medicina Veterinária Preventiva e Saúde Animal (VPS), Faculdade de Medicina Veterinária e Zootecnia (FMVZ), Universidade de São Paulo (USP), Av. Prof. Dr. Orlando Marques de Paiva, 87, São Paulo, SP, Brazil.
authors.forename: Priscilla Anne
authors.initials: PA
authors.lastname: Melville
authors.affiliation: Campus Senador Helvídio Nunes de Barros, Universidade Federal do Piauí, Rua Cícero Eduardo, s/n, Picos, Piauí, Brazil.
authors.forename: Franklin Gerônimo Bispo
authors.initials: FG
authors.lastname: Santos
authors.affiliation: Faculdade de Matemática, Campus Santa Mônica, Universidade Federal de Uberlândia, Av. João Naves de Ávila, 2121, Uberlândia, Minas Gerais, Brazil.
authors.forename: Ednaldo Carvalho
authors.initials: EC
authors.lastname: Guimarães
authors.affiliation: Departamento de Medicina Veterinária Preventiva e Saúde Animal (VPS), Faculdade de Medicina Veterinária e Zootecnia (FMVZ), Universidade de São Paulo (USP), Av. Prof. Dr. Orlando Marques de Paiva, 87, São Paulo, SP, Brazil.
authors.forename: Fábio
authors.initials: F
authors.lastname: Gregori
authors.affiliation: Departamento de Medicina Veterinária Preventiva e Saúde Animal (VPS), Faculdade de Medicina Veterinária e Zootecnia (FMVZ), Universidade de São Paulo (USP), Av. Prof. Dr. Orlando Marques de Paiva, 87, São Paulo, SP, Brazil.
authors.forename: Nilson Roberti
authors.initials: NR
authors.lastname: Benites
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015511:Goat Diseases / Q000382:microbiology*; D006041:Goats / Q000382:microbiology; D008413:Mastitis / Q000382:microbiology / Q000662:veterinary*; D008892:Milk / Q000382:microbiology; D017384:Sequence Deletion; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary*; D013210:Staphylococcus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D037521:Virulence Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D037521:Virulence Factors
keywords: Adhesins; Biofilm formation; Coagulase-negative staphylococci; Coagulase-positive staphylococci; Enterotoxins