title: Chemical characteristics and anti-proliferation activities of Ganoderma tsugae polysaccharides.
abstract: Polysaccharides were extracted by hot-water and hot-alkali from four forms of Ganoderma tsugae including mature and baby Ling chih, mycelium and filtrate. Different profiles of proximate composition and monosaccharide constituents, and element contents were found in the extracted polysaccharides from different extractions and different forms. The molecular weight distributions of polysaccharides were 2.8×10(4)-6.5×10(5)Da and their infrared spectra were comparable. The hot-alkali extracted polysaccharides exhibited better anti-proliferation on IMR32 cells than the hot-water extracted polysaccharides, which were in turn more effective than the hot-water extracts. Besides, most hot-water extracts and both extracted polysaccharides exhibited an anti-proliferation effect on Hep G2 cells. However, the hot-water extracts showed less effective in anti-proliferation of IMR32 and Hep G2 cells. Based on the anti-tumor effects, both polysaccharides could be prepared for use in the formulation of nutraceuticals and functional foods.
authors.affiliation: Department of Food Science and Biotechnology, National Chung Hsing University (NCHU), Taichung, Taiwan, ROC; NCHU/University of California at Davis, Plant and Food Biotechnology Center, NCHU, Taichung, Taiwan, ROC; Agricultural Biotechnology Center, NCHU, Taichung, Taiwan, ROC.
authors.forename: Rao-Chi
authors.initials: RC
authors.lastname: Chien
authors.affiliation: Department of Applied Life Science and Health, Chia Nan University of Pharmacy and Science, Tainan, Taiwan, ROC.
authors.forename: Ming-Tsung
authors.initials: MT
authors.lastname: Yen
authors.affiliation: Department of Culinary Arts, National Kaohsiung University of Hospitality and Tourism, Kaohsiung, Taiwan, ROC.
authors.forename: Yu-Hsiu
authors.initials: YH
authors.lastname: Tseng
authors.affiliation: Department of Food Science and Biotechnology, National Chung Hsing University (NCHU), Taichung, Taiwan, ROC; NCHU/University of California at Davis, Plant and Food Biotechnology Center, NCHU, Taichung, Taiwan, ROC; Agricultural Biotechnology Center, NCHU, Taichung, Taiwan, ROC. Electronic address: jlmau@dragon.nchu.edu.tw.
authors.forename: Jeng-Leun
authors.initials: JL
authors.lastname: Mau
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D032902:Ganoderma; D006801:Humans; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D025282:Mycelium / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D009005:Monosaccharides; D011134:Polysaccharides
keywords: Anti-proliferation; Ganoderma tsugae; Polysaccharide