title: Statistical and population genetics issues of two Hungarian datasets from the aspect of DNA evidence interpretation.
abstract: When the DNA profile from a crime-scene matches that of a suspect, the weight of DNA evidence depends on the unbiased estimation of the match probability of the profiles. For this reason, it is required to establish and expand the databases that reflect the actual allele frequencies in the population applied. 21,473 complete DNA profiles from Databank samples were used to establish the allele frequency database to represent the population of Hungarian suspects. We used fifteen STR loci (PowerPlex ESI16) including five, new ESS loci. The aim was to calculate the statistical, forensic efficiency parameters for the Databank samples and compare the newly detected data to the earlier report. The population substructure caused by relatedness may influence the frequency of profiles estimated. As our Databank profiles were considered non-random samples, possible relationships between the suspects can be assumed. Therefore, population inbreeding effect was estimated using the FIS calculation. The overall inbreeding parameter was found to be 0.0106. Furthermore, we tested the impact of the two allele frequency datasets on 101 randomly chosen STR profiles, including full and partial profiles. The 95% confidence interval estimates for the profile frequencies (pM) resulted in a tighter range when we used the new dataset compared to the previously published ones. We found that the FIS had less effect on frequency values in the 21,473 samples than the application of minimum allele frequency. No genetic substructure was detected by STRUCTURE analysis. Due to the low level of inbreeding effect and the high number of samples, the new dataset provides unbiased and precise estimates of LR for statistical interpretation of forensic casework and allows us to use lower allele frequencies.
authors.affiliation: DNA Lab, Institute of Forensic Medicine, Network of Forensic Science Institutes, Ministry of Justice, Budapest, Hungary.
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Szabolcsi
authors.affiliation: DNA Lab, Institute of Forensic Medicine, Network of Forensic Science Institutes, Ministry of Justice, Budapest, Hungary.
authors.forename: Zsuzsa
authors.initials: Z
authors.lastname: Farkas
authors.affiliation: DNA Lab, Institute of Forensic Medicine, Network of Forensic Science Institutes, Ministry of Justice, Budapest, Hungary.
authors.forename: Andrea
authors.initials: A
authors.lastname: Borbély
authors.affiliation: DNA Lab, Institute of Forensic Medicine, Network of Forensic Science Institutes, Ministry of Justice, Budapest, Hungary.
authors.forename: Gusztáv
authors.initials: G
authors.lastname: Bárány
authors.affiliation: DNA Lab, Institute of Forensic Medicine, Network of Forensic Science Institutes, Ministry of Justice, Budapest, Hungary.
authors.forename: Dániel
authors.initials: D
authors.lastname: Varga
authors.affiliation: DNA Lab, Institute of Forensic Medicine, Network of Forensic Science Institutes, Ministry of Justice, Budapest, Hungary.
authors.forename: Attila
authors.initials: A
authors.lastname: Heinrich
authors.affiliation: DNA Lab, Institute of Forensic Medicine, Network of Forensic Science Institutes, Ministry of Justice, Budapest, Hungary.
authors.forename: Antónia
authors.initials: A
authors.lastname: Völgyi
authors.affiliation: DNA Lab, Institute of Forensic Medicine, Network of Forensic Science Institutes, Ministry of Justice, Budapest, Hungary. Electronic address: phorolma@hotmail.com.
authors.forename: Horolma
authors.initials: H
authors.lastname: Pamjav
mesh_terms: D004247:DNA / Q000235:genetics*; D066264:Datasets as Topic; D053803:Forensic Genetics; D005787:Gene Frequency; D005828:Genetics, Population; D006801:Humans; D006814:Hungary; D018895:Microsatellite Repeats / Q000235:genetics
chemical_list: D004247:DNA
keywords: 21,473 Hungarian samples; Autosomal STRs; DNA interpretation; Population statistics; Structure analysis