title: In vitro and in vivo studies of the endocrine disrupting potency of cadmium in roach (Rutilus rutilus) liver.
abstract: Cadmium has been reported to exert estrogenic, antiestrogenic or both effects in vertebrate species. To elucidate the endocrine disrupting action of CdCl2, ex vivo and in vivo experiments were performed in roach (Rutilus rutilus). Roach liver explants were exposed to a range of CdCl2 concentrations alone (0.1-50μM) or with an effective concentration (100nM) of 17β-estradiol (E2). In addition, juvenile roach were intraperitoneally injected with CdCl2 (0.1-2.5mg/kg) with or without 1mg E2/kg. Subsequent analysis evaluated the effect of CdCl2 on vitellogenin (VTG) synthesis both at the mRNA and protein level, on estrogen receptors (erα and erβ1) and on androgen receptor (ar) mRNA expression. Ex vivo and in vivo experiments indicated that CdCl2 is strongly anti-estrogenic as, when co-exposed to E2, CdCl2 significantly inhibited VTG production as well as vtg and erα mRNA expressions. Moreover, CdCl2 compromised the E2-mediated induction of the ar mRNA expression in vivo.
authors.affiliation: Laboratory of Ecotoxicology, ULH Normandie Université, SFR SACLE 4116, BP 540, 76058 Le Havre, France. Electronic address: marie.gerbron@univ-lehavre.fr.
authors.forename: M
authors.initials: M
authors.lastname: Gerbron
authors.affiliation: Laboratory of Ecotoxicology, ULH Normandie Université, SFR SACLE 4116, BP 540, 76058 Le Havre, France; Akvaplan Niva as, Fram Centre, 9296 Tromsø, Norway.
authors.forename: P
authors.initials: P
authors.lastname: Geraudie
authors.affiliation: Laboratory of Ecotoxicology, ULH Normandie Université, SFR SACLE 4116, BP 540, 76058 Le Havre, France.
authors.forename: B
authors.initials: B
authors.lastname: Xuereb
authors.affiliation: Laboratory of Ecotoxicology, ULH Normandie Université, SFR SACLE 4116, BP 540, 76058 Le Havre, France.
authors.forename: S
authors.initials: S
authors.lastname: Marie
authors.affiliation: Laboratory of Ecotoxicology, ULH Normandie Université, SFR SACLE 4116, BP 540, 76058 Le Havre, France.
authors.forename: C
authors.initials: C
authors.lastname: Minier
mesh_terms: D000818:Animals; D002104:Cadmium / Q000378:metabolism / Q000633:toxicity*; D003530:Cyprinidae / Q000502:physiology*; D052244:Endocrine Disruptors / Q000378:metabolism / Q000633:toxicity*; D004958:Estradiol / Q000378:metabolism / Q000633:toxicity; D047628:Estrogen Receptor alpha / Q000378:metabolism; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011944:Receptors, Androgen / Q000378:metabolism; D011960:Receptors, Estrogen / Q000378:metabolism; D018675:Toxicity Tests; D014819:Vitellogenins / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*
chemical_list: D052244:Endocrine Disruptors; D047628:Estrogen Receptor alpha; D012333:RNA, Messenger; D011944:Receptors, Androgen; D011960:Receptors, Estrogen; D014819:Vitellogenins; D014874:Water Pollutants, Chemical; D002104:Cadmium; D004958:Estradiol
keywords: Cadmium; Endocrine disruption; Liver explants; Roach; Steroid receptors; Vitellogenin