title: Changes in expression of an antimicrobial peptide, digestive enzymes, and nutrient transporters in the intestine of E. praecox-infected chickens.
abstract: Coccidiosis is a major intestinal disease of poultry, caused by several species of the protozoan Eimeria. The objective of this study was to examine changes in expression of digestive enzymes, nutrient transporters, and an antimicrobial peptide following an Eimeria praecox challenge of chickens at days 3 and 6 post-infection. Gene expression was determined by real-time PCR and analyzed by one-way ANOVA. In the duodenum, the primary site of E. praecox infection, a number of genes were downregulated at both d3 and d6 post-infection. These genes included liver expressed antimicrobial peptide 2 (LEAP2), the cationic (CAT1), anionic (EAAT3), and L-type (LAT1) amino acid transporters, the peptide transporter PepT1 and the zinc transporter ZnT1. Other transporters were downregulated either at d3 or d6. At both d3 and d6, there was downregulation of B(o)AT and CAT1 in the jejunum and downregulation of LEAP2 and LAT1 in the ileum. LEAP2, EAAT3, and ZnT1 have been found to be downregulated following challenge with other Eimeria species, suggesting a common cellular response to Eimeria.
authors.affiliation: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu Campus, Chengdu, 611130, Sichuan, China Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg, VA 24061.
authors.forename: H
authors.initials: H
authors.lastname: Yin
authors.affiliation: Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg, VA 24061.
authors.forename: L H
authors.initials: LH
authors.lastname: Sumners
authors.affiliation: Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg, VA 24061.
authors.forename: R A
authors.initials: RA
authors.lastname: Dalloul
authors.affiliation: Animal Biosciences and Biotechnology Laboratory, USDA/ARS, Beltsville MD 20705.
authors.forename: K B
authors.initials: KB
authors.lastname: Miska
authors.affiliation: Animal Parasitic Diseases Laboratory, USDA/ARS, Beltsville, MD 20705.
authors.forename: R H
authors.initials: RH
authors.lastname: Fetterer
authors.affiliation: Animal Parasitic Diseases Laboratory, USDA/ARS, Beltsville, MD 20705.
authors.forename: M C
authors.initials: MC
authors.lastname: Jenkins
authors.affiliation: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu Campus, Chengdu, 611130, Sichuan, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Zhu
authors.affiliation: Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg, VA 24061 ewong@vt.edu.
authors.forename: E A
authors.initials: EA
authors.lastname: Wong
mesh_terms: D000818:Animals; D002645:Chickens; D003048:Coccidiosis / Q000235:genetics / Q000378:metabolism / Q000469:parasitology / Q000662:veterinary*; D004539:Eimeria / Q000502:physiology*; D005786:Gene Expression Regulation; D064451:Hepcidins / Q000235:genetics* / Q000378:metabolism; D007422:Intestines / Q000201:enzymology / Q000469:parasitology; D008297:Male; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D011201:Poultry Diseases / Q000235:genetics* / Q000378:metabolism / Q000469:parasitology; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D064451:Hepcidins; D026901:Membrane Transport Proteins
keywords: Eimeria praecox; liver expressed antimicrobial peptide 2; nutrient transporter