title: Evaluation of two molecularly imprinted polymers for the solid-phase extraction of natural, synthetic and mycoestrogens from environmental water samples before liquid chromatography with mass spectrometry.
abstract: In this work, we have compared the selectivity of two commercial molecularly imprinted polymers (AFFINIMIP®SPE Estrogens and AFFINIMIP®SPE Zearalenone) for the extraction of 12 estrogenic compounds of interest (i.e. 17α-estradiol, 17β-estradiol, estrone, hexestrol, 17α-ethynylestradiol, diethylstibestrol, dienestrol, zearalenone, α-zearalanol, β-zearalanol, α-zearalenol and β-zearalenol) from different water samples. High-performance liquid chromatography coupled with ion trap mass spectrometry with electrospray ionization was used for their determination. Results showed that although both molecularly imprinted polymeric cartridges were specifically designed for different groups of analytes (natural estrogens like estradiol in the first case and zearalenone derivatives in the second) they nearly have the same extraction performance (with recovery values in the range 65-101%) for the same analytes in Milli-Q water because of the cross-reactivity of the polymer. However, when more complex water samples were analyzed, it was clear that the behavior was different and that the AFFINIMIP®SPE Estrogens showed less cross-reactivity than the other cartridge. Validation of the proposed methodology with both cartridges revealed that the extraction was reproducible and that the final limits of detection of the proposed method were in the low ng/L range.
authors.affiliation: Departamento de Química, Unidad Departamental de Química Analítica, Facultad de Ciencias, Universidad de La Laguna (ULL). Tenerife, Islas Canarias, España.
authors.forename: Javier
authors.initials: J
authors.lastname: González-Sálamo
authors.affiliation: Departamento de Química, Unidad Departamental de Química Analítica, Facultad de Ciencias, Universidad de La Laguna (ULL). Tenerife, Islas Canarias, España.
authors.forename: Bárbara
authors.initials: B
authors.lastname: Socas-Rodríguez
authors.affiliation: Departamento de Química, Unidad Departamental de Química Analítica, Facultad de Ciencias, Universidad de La Laguna (ULL). Tenerife, Islas Canarias, España.
authors.forename: Javier
authors.initials: J
authors.lastname: Hernández-Borges
authors.affiliation: Departamento de Química Orgánica, Instituto Universitario de Bio-Orgánica Antonio González, Universidad de La Laguna (ULL). Tenerife, Islas Canarias, España.
authors.forename: María
authors.initials: M
authors.lastname: Del Mar Afonso
authors.affiliation: Departamento de Química, Unidad Departamental de Química Analítica, Facultad de Ciencias, Universidad de La Laguna (ULL). Tenerife, Islas Canarias, España.
authors.forename: Miguel Ángel
authors.initials: MÁ
authors.lastname: Rodríguez-Delgado
keywords: Commercial molecularly imprinted polymers; Estrogens; Liquid chromatography; Mass spectrometry; Water samples