title: Properties of a Bacteriocin Produced by Bacillus subtilis EMD4 Isolated from Ganjang (Soy Sauce).
abstract: A Bacillus species, EMD4, with strong antibacterial activity was isolated from ganjang (soy sauce) and identified as B. subtilis. B. subtilis EMD4 strongly inhibited the growth of B. cereus ATCC14579 and B. thuringiensis ATCC33679. The antibacterial activity was stable at pH 3-9 but inactive at pH 10 and above. The activity was fully retained after 15 min at 80°C but reduced by 50% after 15 min at 90°C. The activity was completely destroyed by proteinase K and protease treatment, indicating its proteinaceous nature. The bacteriocin (BacEMD4) was partially purified from culture supernatant by ammonium sulfate precipitation, and QSepharose and Sephadex G-50 column chromatographies. The specific activity was increased from 769.2 AU/mg protein to 8,347.8 AU/mg protein and the final yield was 12.6%. The size of BacEMD4 was determined to be 3.5 kDa by Tricine SDS-PAGE. The N-terminal amino acid sequence was similar with that of Subtilosin A. Nucleotide sequencing of the cloned gene confirmed that BacEMD4 was Subtilosin A. BacEMD4 showed bactericidal activity against B. cereus ATCC14579.
authors.affiliation: Division of Applied Life Science (BK21 Plus), Graduate School, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Liu
authors.affiliation: Division of Applied Life Science (BK21 Plus), Graduate School, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Jae Yong
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Division of Applied Life Science (BK21 Plus), Graduate School, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Seon-Ju
authors.initials: SJ
authors.lastname: Jeong
authors.affiliation: Department of Food Science, Gyeongnam National University of Science and Technology, Jinju 660-758, Republic of Korea.
authors.forename: Kye Man
authors.initials: KM
authors.lastname: Cho
authors.affiliation: Namhae Garlic Research Institute, Namhae, Gyeongnam 668-812, Republic of Korea.
authors.forename: Gyoung Min
authors.initials: GM
authors.lastname: Kim
authors.affiliation: Namhae Garlic Research Institute, Namhae, Gyeongnam 668-812, Republic of Korea.
authors.forename: Jung-Hye
authors.initials: JH
authors.lastname: Shin
authors.affiliation: School of Food Science and Biotechnology (BK21 Plus), Kyungpook National University, Daegu 702-701, Republic of Korea.
authors.forename: Jong-Sang
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Division of Applied Life Science (BK21 Plus), Graduate School, Gyeongsang National University, Jinju 660-701, Republic of Korea.|Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Jeong Hwan
authors.initials: JH
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D001409:Bacillus cereus / Q000187:drug effects / Q000502:physiology; D001412:Bacillus subtilis / Q000302:isolation & purification* / Q000378:metabolism*; D001413:Bacillus thuringiensis / Q000187:drug effects / Q000502:physiology; D001430:Bacteriocins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D011232:Chemical Precipitation; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D019286:Endopeptidase K / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010456:Peptides, Cyclic / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D059748:Proteolysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D045730:Soy Foods / Q000382:microbiology*; D013696:Temperature
chemical_list: D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010456:Peptides, Cyclic; D012336:RNA, Ribosomal, 16S; C047828:sboA protein, Bacillus subtilis; D019286:Endopeptidase K
keywords: Bacillus subtilis; Ganjang; antimicrobial activity; bacteriocin