title: Bio-ethanol production through simultaneous saccharification and co-fermentation (SSCF) of a low-moisture anhydrous ammonia (LMAA)-pretreated napiegrass (Pennisetum purpureum Schumach).
abstract: Efficient bio-ethanol production from napiegrass (Pennisetum purpureum Schumach) was investigated. A low-moisture anhydrous ammonia (LMAA)-pretreated napiegrass was subjected to simultaneous saccharification and co-fermentation (SSCF), which was performed at 36°C using Escherichia coli KO11, Saccharomyces cerevisiae, cellulase, and xylanase. It was found that use of xylanase as well as the LMAA-pretreatment was effective for the SSCF. After the SSCF for 96 h, the ethanol yield reached 74% of the theoretical yield based on the glucan (397 mg g(-1)) and xylan (214 mg g(-1)) occurring in dry powdered LMAA-pretreated napiergrass.
authors.affiliation: Department of Applied Chemistry, Faculty of Engineering, University of Miyazaki, Gakuen-Kibanadai Nishi, Miyazaki, 889-2192 Japan.
authors.forename: Masahide
authors.initials: M
authors.lastname: Yasuda
authors.affiliation: Department of Applied Chemistry, Faculty of Engineering, University of Miyazaki, Gakuen-Kibanadai Nishi, Miyazaki, 889-2192 Japan.
authors.forename: Hayato
authors.initials: H
authors.lastname: Nagai
authors.affiliation: Department of Applied Chemistry, Faculty of Engineering, University of Miyazaki, Gakuen-Kibanadai Nishi, Miyazaki, 889-2192 Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Takeo
authors.affiliation: Department of Animal and Grassland Sciences, Faculty of Agriculture, University of Miyazaki, Gakuen-Kibanadai Nishi, Miyazaki, 889-2192 Japan.
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Ishii
authors.affiliation: Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen-Kibanadai Nishi, Miyazaki, 889-2192 Japan.
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Ohta
keywords: Bio-ethanol; Cellulase; Escherichia coli KO11; LMAA-pretreatment; Napiergrass; Simultaneous saccharification and co-fermentation (SSCF); Xylanase