title: Effects of zilpaterol hydrochloride on growth performance, blood metabolites, and fatty acid profiles of plasma and adipose tissue in finishing steers.
abstract: The effects of zilpaterol hydrochloride (ZH) on blood metabolites and fatty acid profiles of plasma and adipose tissue were evaluated in crossbred finishing steers (n = 18, BW 639 ± 12.69 kg) that were stratified by BW and randomly assigned, within strata (block), to receive 0 (control) or 8.33 mg/kg diet DM ZH. Cattle were fed once daily ad libitum in individual feeding pens (9 pens/treatment). Zilpaterol hydrochloride was fed for 23 d and withdrawn 3 d before harvest. Blood samples and measures of BW were taken on d 0, 7, 14, and 21. Concentrations of β-hydroxybutyrate (BHB), glucose, and lactate were determined from whole blood. Nonesterified fatty acids, urea nitrogen (PUN), glucose, lactate, and long-chain fatty acid (LCFA) concentrations were analyzed from plasma. Postharvest, adipose tissue samples (approximately 20 g) from subcutaneous fat covering the lumbar vertebrae were collected after 48 h of refrigeration and analyzed for LCFA profiles. Feeding ZH decreased DMI by 8% (P = 0.03) but did not affect BW gain or efficiency (P = 0.83 and P = 0.56, respectively). Addition of ZH resulted in greater HCW, dressing percentage, and LM area ( P = 0.02, P = 0.08, and P = 0.07, respectively) but did not influence other carcass traits (P > 0.10). A ZH × d interaction was observed for PUN and whole-blood glucose concentrations (P = 0.06), in which concentrations decreased in cattle receiving ZH. Nonesterified fatty acids, BHB, plasma glucose, whole-blood, and plasma lactate concentrations were unaffected by ZH (P > 0.10). Zilpaterol hydrochloride increased plasma concentrations of elaidic (P = 0.03), vaccenic (P = 0.006), and docosapentaenoic acids ( P= 0.08), but LCFA concentrations of adipose tissue were unaffected ( P> 0.10), suggesting no preferential oxidation of specific fatty acids. In conclusion, ZH supplementation decreased PUN concentration possibly due to decreased muscle catabolism, but components of blood related to lipid oxidation were unaffected.
authors.forename: C L
authors.initials: CL
authors.lastname: Van Bibber-Krueger
authors.forename: K A
authors.initials: KA
authors.lastname: Miller
authors.forename: G L
authors.initials: GL
authors.lastname: Parsons
authors.forename: L K
authors.initials: LK
authors.lastname: Thompson
authors.forename: J S
authors.initials: JS
authors.lastname: Drouillard
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000097:blood*; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000821:Animal Feed; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001823:Body Composition / Q000187:drug effects / Q000502:physiology; D001835:Body Weight / Q000187:drug effects / Q000502:physiology; D002417:Cattle / Q000254:growth & development* / Q000378:metabolism; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements; D005230:Fatty Acids, Nonesterified / Q000378:metabolism*; D007773:Lactates / Q000097:blood*; D008066:Lipolysis / Q000187:drug effects / Q000502:physiology; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism; D014297:Trimethylsilyl Compounds / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D007773:Lactates; D014297:Trimethylsilyl Compounds; C475840:Zilpaterol; D020155:3-Hydroxybutyric Acid