title: Production of optically pure L-phenyllactic acid by using engineered Escherichia coli coexpressing L-lactate dehydrogenase and formate dehydrogenase.
abstract: L-Phenyllactic acid (L-PLA) is a novel antiseptic agent with broad and effective antimicrobial activity. In addition, L-PLA has been used for synthesis of poly(phenyllactic acid)s, which exhibits better mechanical properties than poly(lactic acid)s. However, the concentration and optical purity of L-PLA produced by native microbes was rather low. An NAD-dependent L-lactate dehydrogenase (L-nLDH) from Bacillus coagulans NL01 was confirmed to have a good ability to produce L-PLA from phenylpyruvic acid (PPA). In the present study, l-nLDH gene and formate dehydrogenase gene were heterologously coexpressed in Escherichia coli. Through two coupled reactions, 79.6mM l-PLA was produced from 82.8mM PPA in 40min and the enantiomeric excess value of L-PLA was high (>99%). Therefore, this process suggested a promising alternative for the production of chiral l-PLA.
authors.affiliation: College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, People's Republic of China; Jiangsu Key Lab of Biomass-based Green Fuels and Chemicals, Nanjing 210037, People's Republic of China.
authors.forename: Zhaojuan
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, People's Republic of China.
authors.forename: Mingyue
authors.initials: M
authors.lastname: Zhao
authors.affiliation: College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zang
authors.affiliation: College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, People's Republic of China; Jiangsu Key Lab of Biomass-based Green Fuels and Chemicals, Nanjing 210037, People's Republic of China. Electronic address: hgouyj@njfu.edu.cn.
authors.forename: Jia
authors.initials: J
authors.lastname: Ouyang
mesh_terms: D001407:Bacillus / Q000235:genetics; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005560:Formate Dehydrogenases / Q000235:genetics / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D019344:Lactic Acid / Q000096:biosynthesis*; D010667:Phenylpyruvic Acids / Q000737:chemistry
chemical_list: D010667:Phenylpyruvic Acids; D019344:Lactic Acid; D007770:L-Lactate Dehydrogenase; D005560:Formate Dehydrogenases; C031606:phenylpyruvic acid
keywords: Coexpression; Formate dehydrogenase; NAD-dependent l-lactate dehydrogenase; Optical purity; l-Phenyllactic acid