title: Cytotoxic Potential and Molecular Characterization of Fungal Endophytes from Selected High Value Medicinal Plants of the Kashmir Valley - India.
abstract: The present study explores the fungal endophytes from selected high value medicinal plants to check their activities at in-vitro and in-vivo level. The in-vitro cytotoxicity of selected endophytes revealed potent growth inhibition against human cancer cell lines of leukemia (THP-1), lung (A549), prostate (PC-3), colon (Caco-2), neuroblastoma (IMR-32) and breast (MCF-7) at a concentration of 100 µg/ml. Among them the endophytic strains I. e., IIIM2, IIIM3, IIIM7 and IIIM8 showed most significant growth inhibition against colon (Caco-2), prostate (PC-3), lung (A549) and leukemia (THP-1) cancer cell lines. At the in-vivo level maximum (58.95%) tumor growth inhibition was documented with the extract of IIIM2 against Ehrlich Ascites Carcinoma mouse modal. All the potent fungal endophytic strains were characterized using ITS 4 and ITS 5 region sequencing and phylogenetic analysis was ascertained among them. This paper confirms the 2 elite endophytic fungal strains, IIIM2 and IIIM8, have the potential to act as a source of new anticancer compounds.
authors.affiliation: Biotechnology Division, CSIR - Indian Institute of Integrative Medicine, Sanatnagar - Srinagar, Kashmir, India.
authors.forename: R A
authors.initials: RA
authors.lastname: Dar
authors.affiliation: Biotechnology Division, CSIR - Indian Institute of Integrative Medicine, Sanatnagar - Srinagar, Kashmir, India.
authors.forename: P H
authors.initials: PH
authors.lastname: Qazi
authors.affiliation: Biotechnology Division, CSIR - Indian Institute of Integrative Medicine, Sanatnagar - Srinagar, Kashmir, India.
authors.forename: I
authors.initials: I
authors.lastname: Saba
authors.affiliation: Biotechnology Division, CSIR - Indian Institute of Integrative Medicine, Sanatnagar - Srinagar, Kashmir, India.
authors.forename: S A
authors.initials: SA
authors.lastname: Rather
authors.affiliation: Cancer Pharmacology Division, CSIR - Indian Institute of Integrative Medicine, Jammu - Tawi, India.
authors.forename: Z A
authors.initials: ZA
authors.lastname: Wani
authors.affiliation: Cancer Pharmacology Division, CSIR - Indian Institute of Integrative Medicine, Jammu - Tawi, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Qazi
authors.affiliation: Biotechnology Division, CSIR - Indian Institute of Integrative Medicine, Sanatnagar - Srinagar, Kashmir, India.
authors.forename: A A
authors.initials: AA
authors.lastname: Shiekh
authors.affiliation: Biotechnology Division, CSIR - Indian Institute of Integrative Medicine, Sanatnagar - Srinagar, Kashmir, India.
authors.forename: A
authors.initials: A
authors.lastname: Manzoor
authors.affiliation: Cancer Pharmacology Division, CSIR - Indian Institute of Integrative Medicine, Jammu - Tawi, India.
authors.forename: A
authors.initials: A
authors.lastname: Hamid
authors.affiliation: Cancer Pharmacology Division, CSIR - Indian Institute of Integrative Medicine, Jammu - Tawi, India.
authors.forename: D M
authors.initials: DM
authors.lastname: Modae
mesh_terms: D000072283:A549 Cells; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology / Q000627:therapeutic use; D018938:Caco-2 Cells; D045744:Cell Line, Tumor; D060026:Endophytes / Q000737:chemistry*; D005658:Fungi / Q000737:chemistry*; D006801:Humans; D007194:India; D061986:MCF-7 Cells; D051379:Mice; D010802:Phylogeny; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology* / Q000627:therapeutic use*; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D010936:Plant Extracts