title: A highly selective fluorescent sensor for Al³⁺ and the use of the resulting complex as a secondary sensor for PPi in aqueous media: its applicability in live cell imaging.
abstract: An easy-to-make salicylimine (L) bearing an "O-N-O"-coordination site was used as a highly selective fluorescent sensor for Al(3+) and PPi in aqueous solution. Sensor L showed a significant fluorescence enhancement in the presence of Al(3+) over other competitive metal ions. It works based on the Al(3+)-induced formation of a 1 : 1 L-Al(3+) complex, producing a chelation-enhanced fluorescence effect, the fluorescence quantum yield reached 0.59. This L-Al(3+) ensemble is a subsequent fluorescent sensor for PPi due to the strong attraction between Al(3+) and PPi, it can selectively discriminate PPi overcoming the interference of the biological competitors including PO4(3-), ADP and ATP at physiological pH. L and L-Al(3+) exhibit high sensitivity and selectivity for Al(3+) and PPi, the detection limits were found to be as low as 2.94 × 10(-8) M and 2.74 × 10(-7) M, respectively. It was further confirmed that sensor L had potential practical applications through mapping of Al(3+) in live cells.
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, Jilin University, 2699 Qianjin Avenue, Changchun 130012, P. R. China. smjiang@jlu.edu.cn.
authors.forename: Chunshuang
authors.initials: C
authors.lastname: Liang
authors.forename: Wenhuan
authors.initials: W
authors.lastname: Bu
authors.forename: Chenglong
authors.initials: C
authors.lastname: Li
authors.forename: Guangwen
authors.initials: G
authors.lastname: Men
authors.forename: Mengyu
authors.initials: M
authors.lastname: Deng
authors.forename: Yukun
authors.initials: Y
authors.lastname: Jiangyao
authors.forename: Hongchen
authors.initials: H
authors.lastname: Sun
authors.forename: Shimei
authors.initials: S
authors.lastname: Jiang
mesh_terms: D000535:Aluminum / Q000032:analysis* / Q000737:chemistry; D056831:Coordination Complexes / Q000737:chemistry* / Q000494:pharmacology; D011756:Diphosphates / Q000032:analysis* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D007097:Imines / Q000737:chemistry* / Q000494:pharmacology; D008856:Microscopy, Fluorescence
chemical_list: D056831:Coordination Complexes; D011756:Diphosphates; D005456:Fluorescent Dyes; D007097:Imines; C107241:diphosphoric acid; D000535:Aluminum