title: Alternative solution for ex vivo lung perfusion, experimental study on donated human lungs non-accepted for transplantation.
abstract: OBJECTIVE
  To evaluate a new perfusate solution to be used for ex vivo lung perfusion.
  
  
  METHODS
  Randomized experimental study using lungs from rejected brain-dead donors harvested and submitted to 1 hour of ex vivo lung perfusion (EVLP) using mainstream solution or the alternative.
  
  
  RESULTS
  From 16 lungs blocs tested, we found no difference on weight after EVLP: Steen group (SG) = 1,097±526g; Alternative Perfusion Solution (APS) = 743±248g, p=0.163. Edema formation, assessed by Wet/dry weigh ratio, was statistically higher on the Alternative Perfusion Solution group (APS = 3.63 ± 1.26; SG = 2.06 ± 0.28; p = 0.009). No difference on PaO2 after EVLP (SG = 498±37.53mmHg; APS = 521±55.43mmHg, p=0.348, nor on histological analyses: pulmonary injury score: SG = 4.38±1.51; APS = 4.50±1.77, p=0.881; apoptotic cells count after perfusion: SG = 2.4 ± 2.0 cells/mm2; APS = 4.8 ± 6.9 cells/mm2; p = 0.361).
  
  
  CONCLUSIONS
  The ex vivo lung perfusion using the alternative perfusion solution showed no functional or histological differences, except for a higher edema formation, from the EVLP using Steen Solution(r) on lungs from rejected brain-dead donors.
authors.affiliation: School of Medicine, Sao Paulo University, Brazil.
authors.forename: Lucas Matos
authors.initials: LM
authors.lastname: Fernandes
authors.affiliation: School of Medicine, USP, Sao Paulo, SP, Brazil.
authors.forename: Alessandro Wasum
authors.initials: AW
authors.lastname: Mariani
authors.affiliation: Department of Thoracic Surgery, Messejana Hospital, Fortaleza, CE, Brazil.
authors.forename: Israel Lopes de
authors.initials: IL
authors.lastname: Medeiros
authors.affiliation: School of Medicine, USP, Sao Paulo, SP, Brazil.
authors.forename: Marcos Naoyuki
authors.initials: MN
authors.lastname: Samano
authors.affiliation: School of Medicine, USP, Sao Paulo, SP, Brazil.
authors.forename: Luís Gustavo
authors.initials: LG
authors.lastname: Abdalla
authors.affiliation: School of Medicine, USP, Sao Paulo, SP, Brazil.
authors.forename: Aristides Tadeu
authors.initials: AT
authors.lastname: Correia
authors.affiliation: School of Medicine, USP, Sao Paulo, SP, Brazil.
authors.forename: Natália Aparecida
authors.initials: NA
authors.lastname: Nepomuceno
authors.affiliation: School of Medicine, USP, Sao Paulo, SP, Brazil.
authors.forename: Mauro
authors.initials: M
authors.lastname: Canzian
authors.affiliation: School of Medicine, USP, Sao Paulo, SP, Brazil.
authors.forename: Paulo Manuel
authors.initials: PM
authors.lastname: Pêgo-Fernandes
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D005112:Extracorporeal Circulation / Q000379:methods; D005260:Female; D006801:Humans; D008168:Lung / Q000098:blood supply*; D016040:Lung Transplantation / Q000379:methods*; D008297:Male; D008875:Middle Aged; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D010477:Perfusion / Q000379:methods*; D015427:Reperfusion Injury; D015203:Reproducibility of Results; D018709:Statistics, Nonparametric; D013997:Time Factors; D014019:Tissue Donors; D020858:Tissue and Organ Harvesting / Q000379:methods; D055815:Young Adult
chemical_list: D019188:Organ Preservation Solutions