title: Nanobarcoded superparamagnetic iron oxide nanoparticles for nanomedicine: Quantitative studies of cell-nanoparticle interactions by scanning image cytometry.
abstract: Oligonucleotide-functionalized nanoparticles (NPs) are promising agents for nanomedicine, but the potential in vitro nanotoxicity that may arise from such conjugates has yet to be evaluated in a dose response manner. Since nanomedicine functions on the single-cell level, measurements of nanotoxicity should also be performed as such. In vitro single-cell nanotoxicity assays based on scanning image cytometry are used to study a specific type of oligo-functionalized NP, "nanobarcoded" superparamagnetic iron oxide NPs (NB-SPIONs). The selected panel of single-cell assays measures well-known modes of nanotoxicity--apoptosis, necrosis, generation of reactive oxygen species (ROS), and cell number. Using these assays, the cytotoxicity of two sizes of NB-SPIONs (10 nm and 30 nm core size) was compared to the parent NP, carboxylated SPIONs (COOH-SPIONs). The results suggest that the conjugated NB confers a biocompatible coating that protects against cytotoxicity at very high SPION doses, but both NB- and COOH-SPIONs of either size generally have low in vitro cytotoxicity at physiologically relevant doses.
authors.affiliation: Weldon School of Biomedical Engineering, Birck Nanotechnology Center, Purdue University, West Lafayette, Indiana.
authors.forename: Trisha
authors.initials: T
authors.lastname: Eustaquio
authors.affiliation: Weldon School of Biomedical Engineering, Birck Nanotechnology Center, Bindley Bioscience Center, School of Veterinary Medicine, Department of Basic Medical Sciences, Purdue University, West Lafayette, Indiana.
authors.forename: James F
authors.initials: JF
authors.lastname: Leary
mesh_terms: D017209:Apoptosis; D002470:Cell Survival; D006367:HeLa Cells; D006801:Humans; D019044:Image Cytometry; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D050997:Nanomedicine
chemical_list: D058185:Magnetite Nanoparticles
keywords: apoptosis; image analysis; nanoparticles; nanotoxicity; necrosis; reactive oxygen species