title: Anti-Adhesive Activity of Cranberry Phenolic Compounds and Their Microbial-Derived Metabolites against Uropathogenic Escherichia coli in Bladder Epithelial Cell Cultures.
abstract: Cranberry consumption has shown prophylactic effects against urinary tract infections (UTI), although the mechanisms involved are not completely understood. In this paper, cranberry phenolic compounds and their potential microbial-derived metabolites (such as simple phenols and benzoic, phenylacetic and phenylpropionic acids) were tested for their capacity to inhibit the adherence of uropathogenic Escherichia coli (UPEC) ATCC®53503™ to T24 epithelial bladder cells. Catechol, benzoic acid, vanillic acid, phenylacetic acid and 3,4-dihydroxyphenylacetic acid showed anti-adhesive activity against UPEC in a concentration-dependent manner from 100-500 µM, whereas procyanidin A2, widely reported as an inhibitor of UPEC adherence on uroepithelium, was only statistically significant (p < 0.05) at 500 µM (51.3% inhibition). The results proved for the first time the anti-adhesive activity of some cranberry-derived phenolic metabolites against UPEC in vitro, suggesting that their presence in the urine could reduce bacterial colonization and progression of UTI.
authors.affiliation: Institute of Food Science Research (CIAL), CSIC-UAM, C/Nicolás Cabrera 9, 28049 Madrid, Spain. d.g.dellano@csic.es.
authors.forename: Dolores González
authors.initials: DG
authors.lastname: de Llano
authors.affiliation: Institute of Food Science Research (CIAL), CSIC-UAM, C/Nicolás Cabrera 9, 28049 Madrid, Spain. adelaida.e.fernandez@cial.uam-csic.es.
authors.forename: Adelaida
authors.initials: A
authors.lastname: Esteban-Fernández
authors.affiliation: Institute of Food Science Research (CIAL), CSIC-UAM, C/Nicolás Cabrera 9, 28049 Madrid, Spain. f.s.patan@csic.es.
authors.forename: Fernando
authors.initials: F
authors.lastname: Sánchez-Patán
authors.affiliation: Institute of Food Science Research (CIAL), CSIC-UAM, C/Nicolás Cabrera 9, 28049 Madrid, Spain. pedroj.martin.alvarez@csic.es.
authors.forename: Pedro J
authors.initials: PJ
authors.lastname: Martínlvarez
authors.affiliation: Institute of Food Science Research (CIAL), CSIC-UAM, C/Nicolás Cabrera 9, 28049 Madrid, Spain. victoria.moreno@csic.es.
authors.forename: Maria Victoria
authors.initials: MV
authors.lastname: Moreno-Arribas
authors.affiliation: Institute of Food Science Research (CIAL), CSIC-UAM, C/Nicolás Cabrera 9, 28049 Madrid, Spain. b.bartolome@csic.es.
authors.forename: Begoña
authors.initials: B
authors.lastname: Bartolomé
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000382:microbiology; D006801:Humans; D010636:Phenols / Q000737:chemistry* / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D001743:Urinary Bladder / Q000166:cytology / Q000187:drug effects* / Q000382:microbiology; D056689:Uropathogenic Escherichia coli / Q000187:drug effects* / Q000502:physiology; D029799:Vaccinium macrocarpon / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D010636:Phenols; D010936:Plant Extracts
keywords: T-24 cells; UPEC; UTI; flavan-3-ols; phenolic metabolites; urinary tract infection; uropathogenic Escherichia coli