title: Assessment of intensive care unit-acquired weakness in young and old mice: An E. coli septic peritonitis model.
abstract: BACKGROUND
  There are few reports of in vivo muscle strength measurements in animal models of ICU-acquired weakness (ICU-AW). In this study we investigated whether the Escherichia coli (E. coli) septic peritonitis mouse model may serve as an ICU-AW model using in vivo strength measurements and myosin/actin assays, and whether development of ICU-AW is age-dependent in this model.
  
  
  METHODS
  Young and old mice were injected intraperitoneally with E. coli and treated with ceftriaxone. Forelimb grip strength was measured at multiple time points, and the myosin/actin ratio in muscle was determined.
  
  
  RESULTS
  E. coli administration was not associated with grip strength decrease, neither in young nor in old mice. In old mice, the myosin/actin ratio was lower in E. coli mice at t = 48 h and higher at t = 72 h compared with controls.
  
  
  CONCLUSIONS
  This E. coli septic peritonitis mouse model did not induce decreased grip strength. In its current form, it seems unsuitable as a model for ICU-AW.
authors.affiliation: Department of Intensive Care Medicine, Academic Medical Center, Room G3-228, PO box 22700, Meibergdreef 9, 1105 AZ, Amsterdam, the Netherlands.|Laboratory of Experimental Intensive Care and Anesthesiology (L·E·I·C·A), Academic Medical Center, Amsterdam, the Netherlands.
authors.forename: Esther
authors.initials: E
authors.lastname: Witteveen
authors.affiliation: Department of Neurology, Academic Medical Center, Amsterdam, the Netherlands.|Center for Experimental Molecular Medicine, Academic Medical Center, Amsterdam, the Netherlands.
authors.forename: Inge C M
authors.initials: IC
authors.lastname: Hoogland
authors.affiliation: Department of Intensive Care Medicine, Academic Medical Center, Room G3-228, PO box 22700, Meibergdreef 9, 1105 AZ, Amsterdam, the Netherlands.|Laboratory of Experimental Intensive Care and Anesthesiology (L·E·I·C·A), Academic Medical Center, Amsterdam, the Netherlands.|Department of Neurology, Academic Medical Center, Amsterdam, the Netherlands.
authors.forename: Luuk
authors.initials: L
authors.lastname: Wieske
authors.affiliation: Laboratory of Experimental Intensive Care and Anesthesiology (L·E·I·C·A), Academic Medical Center, Amsterdam, the Netherlands.
authors.forename: Nina C
authors.initials: NC
authors.lastname: Weber
authors.affiliation: Department of Neurology, Academic Medical Center, Amsterdam, the Netherlands.
authors.forename: Camiel
authors.initials: C
authors.lastname: Verhamme
authors.affiliation: Department of Intensive Care Medicine, Academic Medical Center, Room G3-228, PO box 22700, Meibergdreef 9, 1105 AZ, Amsterdam, the Netherlands.|Laboratory of Experimental Intensive Care and Anesthesiology (L·E·I·C·A), Academic Medical Center, Amsterdam, the Netherlands.
authors.forename: Marcus J
authors.initials: MJ
authors.lastname: Schultz
authors.affiliation: Department of Neurology, Academic Medical Center, Amsterdam, the Netherlands.
authors.forename: Ivo N
authors.initials: IN
authors.lastname: van Schaik
authors.affiliation: Department of Intensive Care Medicine, Academic Medical Center, Room G3-228, PO box 22700, Meibergdreef 9, 1105 AZ, Amsterdam, the Netherlands.|Laboratory of Experimental Intensive Care and Anesthesiology (L·E·I·C·A), Academic Medical Center, Amsterdam, the Netherlands.
authors.forename: Janneke
authors.initials: J
authors.lastname: Horn
mesh_terms: D000199:Actins / Q000378:metabolism; D000367:Age Factors; D000375:Aging; D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D001835:Body Weight; D002443:Ceftriaxone / Q000627:therapeutic use; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000472:pathogenicity*; D007362:Intensive Care Units; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018908:Muscle Weakness / Q000209:etiology / Q000451:nursing*; D018482:Muscle, Skeletal / Q000378:metabolism / Q000473:pathology; D009218:Myosins / Q000378:metabolism; D010538:Peritonitis / Q000150:complications / Q000628:therapy*
chemical_list: D000199:Actins; D000900:Anti-Bacterial Agents; D002443:Ceftriaxone; D009218:Myosins
keywords: animal model; critical illness myopathy; critical illness polyneuropathy; grip strength; intensive care unit-acquired weakness