title: Pharmacokinetic study of isatin in dog plasma by liquid chromatography tandem mass spectrometry.
abstract: OBJECTIVE
  A sensitive and selective method was developed and validated to study the pharmacokinetics of isatin.
  
  
  METHODS
  The blood samples were pretreated by protein precipitation method using methanol. Quetiapine was used as an internal standard. After pretreatment, the samples were assayed by LC/MS/MS method and the pharmacokinetic parameters were calculated by WinNonlin 5.2 using non-compartment model. The separation was performed on a Venusil XBP PH column (5 µm, 2.0×100 mm) with an isocratic mobile phase consisted of methanol-water (containing 50 mM ammonium formate) (65:35, v/v) at a flow rate of 0.3 mL/min. The Agilent G6410B triple quadrupole LC/MS system was operated under the multiple reactions monitoring mode (MRM) using the electrospray ionization technique in positive mode.
  
  
  RESULTS
  The lower limits of quantification (LLOQ) of the analyte of the method was 10 ng/mL. The method was linear with correlation coefficient >0.995. The intraday and interday accuracy and precision of the assay were acceptable.
  
  
  CONCLUSIONS
  This method has been applied successfully to a pharmacokinetic study involving the oral and intravenous administration of isatin to beagle dogs.
authors.affiliation: Shandong Provincial Hospital affiliated to Shandong University, Jinan, China - 1471128187@qq.com.
authors.forename: A
authors.initials: A
authors.lastname: Ren
authors.forename: Q
authors.initials: Q
authors.lastname: Wang
authors.forename: Z
authors.initials: Z
authors.lastname: Fang
authors.forename: M
authors.initials: M
authors.lastname: Gao
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.forename: W
authors.initials: W
authors.lastname: Xu
authors.forename: W
authors.initials: W
authors.lastname: Yue
authors.forename: L
authors.initials: L
authors.lastname: Yin
authors.forename: Z
authors.initials: Z
authors.lastname: Liu
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: B
authors.initials: B
authors.lastname: Ding
mesh_terms: D000818:Animals; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D004285:Dogs; D005260:Female; D007510:Isatin / Q000097:blood* / Q000493:pharmacokinetics; D057230:Limit of Detection; D008297:Male; D008996:Monoamine Oxidase Inhibitors / Q000097:blood* / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D008996:Monoamine Oxidase Inhibitors; D007510:Isatin