title: Term pregnancy and live birth subsequent to immediate uterine transfer of sperm microinjected oocyte in a natural cycle.
abstract: It has been reported that it is possible to achieve a pregnancy after immediate uterine transfer of oocyte and sperm, before fertilization and cleavage were known to have occurred; there is an enormous amount of work about the optimal timing of embryo transfer, with no conclusive evidence of a gold standard satisfying patient age, endometrial receptivity, hormonal levels and embryological parameters. We hereby report a case of one 35-year-old nulligravid woman with longstanding tubal factor infertility and 3 previous failed ICSI cycles, treated with ICSI and immediate transfer of the oocyte microinjected with a spermatozoon in a natural cycle. A single oocyte was retrieved, injected with a spermatozoon and transferred 40 min after injection, resulting in an uneventful pregnancy and delivery of a healthy female infant weighing 3320 g at 40 weeks' estimated gestational age. This case, certainly novel, should be interpreted with caution. Whether confirmed for efficacy and safeness in appropriate controlled clinical trials, our present observation could offer a simple, practical and cost-effective approach in ART programs in selected patients.
authors.affiliation: a Division of Sterility and Assisted Reproduction, Department of Gynecological-Obstetric Sciences and Urologic Sciences, Umberto I Policlinic of Rome, Sapienza , University of Rome , Roma , Italy.
authors.forename: Cesare
authors.initials: C
authors.lastname: Aragona
authors.affiliation: a Division of Sterility and Assisted Reproduction, Department of Gynecological-Obstetric Sciences and Urologic Sciences, Umberto I Policlinic of Rome, Sapienza , University of Rome , Roma , Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Linari
authors.affiliation: a Division of Sterility and Assisted Reproduction, Department of Gynecological-Obstetric Sciences and Urologic Sciences, Umberto I Policlinic of Rome, Sapienza , University of Rome , Roma , Italy.
authors.forename: Giulietta
authors.initials: G
authors.lastname: Micara
authors.affiliation: a Division of Sterility and Assisted Reproduction, Department of Gynecological-Obstetric Sciences and Urologic Sciences, Umberto I Policlinic of Rome, Sapienza , University of Rome , Roma , Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Tranquilli
authors.affiliation: a Division of Sterility and Assisted Reproduction, Department of Gynecological-Obstetric Sciences and Urologic Sciences, Umberto I Policlinic of Rome, Sapienza , University of Rome , Roma , Italy.
authors.forename: Agnese Maria Lourdes
authors.initials: AM
authors.lastname: Gambaro
authors.affiliation: a Division of Sterility and Assisted Reproduction, Department of Gynecological-Obstetric Sciences and Urologic Sciences, Umberto I Policlinic of Rome, Sapienza , University of Rome , Roma , Italy.
authors.forename: Maria Salome
authors.initials: MS
authors.lastname: Bezerra Espinola
mesh_terms: D000328:Adult; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D050498:Live Birth; D009865:Oocytes / Q000637:transplantation*; D011247:Pregnancy; D011256:Pregnancy Outcome; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*
keywords: ICSI; immediate transfer; natural cycle; poor responder