title: Effect of targeted delivery of bone morphogenetic protein-2 on bone formation in type 1 diabetes.
abstract: OBJECTIVE
  Bone formation and healing are diminished in experimental type 1 diabetes. The present study investigated whether controlled local release of recombinant human bone morphogenetic protein 2 (rhBMP-2) stimulates bone defect healing in diabetes as a consequence of its anabolic effects on bone.
  
  
  METHODS
  Bilateral experimental circular bone defects were created in the temporal bones of 64 BALB/cByJ mice. Defects were treated with acellular collagen sponge plus 0.4 or 1.8 μg of rhBMP-2 per defect, and untreated defects served as controls. The healing of the defects over a 14-day period in diabetic and nondiabetic mice was analyzed histomorphometrically.
  
  
  RESULTS
  Diabetes inhibited bone formation in both untreated and BMP-treated bone defects. Controlled local release of rhBMP-2 significantly stimulated bone formation in diabetic animals, bringing it nearly to normal levels, and enhanced bone regeneration in normal animals.
  
  
  CONCLUSIONS
  Recombinant human BMP-2 may be beneficial in treating deficient intramembranous bone formation in diabetes.
authors.forename: Ronaldo Barcellos
authors.initials: RB
authors.lastname: de Santana
authors.forename: Phillip C
authors.initials: PC
authors.lastname: Trackman
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000737:chemistry; D001847:Bone Diseases / Q000150:complications / Q000517:prevention & control*; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry* / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects; D001842:Bone and Bones / Q000187:drug effects; D003094:Collagen / Q000737:chemistry; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000188:drug therapy*; D003922:Diabetes Mellitus, Type 1 / Q000150:complications / Q000188:drug therapy*; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010012:Osteogenesis / Q000187:drug effects*; D011994:Recombinant Proteins / Q000494:pharmacology; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects
chemical_list: C525718:BMP2 protein, human; D001786:Blood Glucose; D055396:Bone Morphogenetic Protein 2; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; D003094:Collagen