title: Determining the geometry of oligomers of the human epidermal growth factor family on cells with <10 nm resolution.
abstract: There is a limited range of methods available to characterize macromolecular organization in cells on length scales from 5-50 nm. We review methods currently available and show the latest results from a new single-molecule localization-based method, fluorophore localization imaging with photobleaching (FLImP), using the epidermal growth factor (EGF) receptor (EGFR) as an example system. Our measurements show that FLImP is capable of achieving spatial resolution in the order of 6 nm.
authors.affiliation: *Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Harwell Oxford, Didcot, Oxfordshire, OX11 0FA, U.K.
authors.forename: Sarah R
authors.initials: SR
authors.lastname: Needham
authors.affiliation: *Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Harwell Oxford, Didcot, Oxfordshire, OX11 0FA, U.K.
authors.forename: Laura C
authors.initials: LC
authors.lastname: Zanetti-Domingues
authors.affiliation: *Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Harwell Oxford, Didcot, Oxfordshire, OX11 0FA, U.K.
authors.forename: Kathrin M
authors.initials: KM
authors.lastname: Scherer
authors.affiliation: *Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Harwell Oxford, Didcot, Oxfordshire, OX11 0FA, U.K.
authors.forename: Michael
authors.initials: M
authors.lastname: Hirsch
authors.affiliation: *Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Harwell Oxford, Didcot, Oxfordshire, OX11 0FA, U.K.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Rolfe
authors.affiliation: *Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Harwell Oxford, Didcot, Oxfordshire, OX11 0FA, U.K.
authors.forename: Selene K
authors.initials: SK
authors.lastname: Roberts
authors.affiliation: *Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Harwell Oxford, Didcot, Oxfordshire, OX11 0FA, U.K.
authors.forename: Marisa L
authors.initials: ML
authors.lastname: Martin-Fernandez
authors.affiliation: *Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Harwell Oxford, Didcot, Oxfordshire, OX11 0FA, U.K.
authors.forename: David T
authors.initials: DT
authors.lastname: Clarke
authors.affiliation: *Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Harwell Oxford, Didcot, Oxfordshire, OX11 0FA, U.K.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Tynan
mesh_terms: D004815:Epidermal Growth Factor / Q000737:chemistry*; D066246:ErbB Receptors / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D046911:Macromolecular Substances / Q000737:chemistry*; D055503:Protein Multimerization
chemical_list: D005456:Fluorescent Dyes; D046911:Macromolecular Substances; D004815:Epidermal Growth Factor; C512478:EGFR protein, human; D066246:ErbB Receptors