title: Hemato-immunological and biochemical parameters of silver catfish Rhamdia quelen immunized with live theronts of Ichthyophthirius multifiliis.
abstract: This study investigated the immunization by intraperitoneal injection (i.p.) (assay I) and immersion bath (assay II) with live theronts of Ichthyophthirius multifillis in Rhamdia quelen and its influence on the hemato-immunological and biochemical parameters. Fish were divided in control (non immunized no challenged); non immunized and challenged with 12,000 theronts/fish; non immunized and challenged with 22,000 theronts/fish; immunized and challenged with 12,000 theronts/fish; immunized and challenged with 22,000 theronts/fish. Six days after challenge, either in the assay I or in the assay II the prevalence of I. multifillis in the gills was higher in non immunized fish (33.33% and 27.77%, respectively). In the assay I showed higher numbers of thrombocytes, leukocytes, lymphocytes, neutrophils and monocytes 20 days after injection and lower numbers after challenge. The immunoglobulin values were higher in fish non immunized. Fish immunized by immersion bath (assay II) showed greater values of catalase (CAT) in the liver (1245.49 U/mgprt) when compared to i.p. (198.79 U/mgprt). The levels of CAT in the liver of fish from the assay II were greater (1738.47 U/mgprt) 14 days after immunization than that observed 21 days after (1114.26 U/mgprt). The vaccination by i.p method showed influence on the hematological parameters. On the other hand, the immersion bath vaccination showed greater influence on the catalase activity in the liver. The results showed that new parameters like total protein, immunoglobulin and antioxidant enzymes could be considered in evaluating the host response to infection.
authors.affiliation: AQUOS - Aquatic Organisms Health Laboratory, Aquaculture Department, Federal University of Santa Catarina (UFSC), Rod. Admar Gonzaga 1346, 88040-900, Florianópolis, Santa Catarina, Brazil. Electronic address: roberta.tancredo@gmail.com.
authors.forename: Karen R
authors.initials: KR
authors.lastname: Tancredo
authors.affiliation: LAPON - Laboratório de Piscicultura Marinha Ornamental, Aquaculture Department, Federal University of Santa Catarina (UFSC), Rod. Admar Gonzaga 1346, 88040-900, Florianópolis, Santa Catarina, Brazil.
authors.forename: Eduardo L T
authors.initials: EL
authors.lastname: Gonçalves
authors.affiliation: AQUOS - Aquatic Organisms Health Laboratory, Aquaculture Department, Federal University of Santa Catarina (UFSC), Rod. Admar Gonzaga 1346, 88040-900, Florianópolis, Santa Catarina, Brazil.
authors.forename: Aline
authors.initials: A
authors.lastname: Brum
authors.affiliation: AQUOS - Aquatic Organisms Health Laboratory, Aquaculture Department, Federal University of Santa Catarina (UFSC), Rod. Admar Gonzaga 1346, 88040-900, Florianópolis, Santa Catarina, Brazil.
authors.forename: Monyele
authors.initials: M
authors.lastname: Acchile
authors.affiliation: AQUOS - Aquatic Organisms Health Laboratory, Aquaculture Department, Federal University of Santa Catarina (UFSC), Rod. Admar Gonzaga 1346, 88040-900, Florianópolis, Santa Catarina, Brazil.
authors.forename: Gabriela S O
authors.initials: GS
authors.lastname: Hashimoto
authors.affiliation: AQUOS - Aquatic Organisms Health Laboratory, Aquaculture Department, Federal University of Santa Catarina (UFSC), Rod. Admar Gonzaga 1346, 88040-900, Florianópolis, Santa Catarina, Brazil.
authors.forename: Scheila A
authors.initials: SA
authors.lastname: Pereira
authors.affiliation: AQUOS - Aquatic Organisms Health Laboratory, Aquaculture Department, Federal University of Santa Catarina (UFSC), Rod. Admar Gonzaga 1346, 88040-900, Florianópolis, Santa Catarina, Brazil.
authors.forename: Maurício L
authors.initials: ML
authors.lastname: Martins
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood / Q000276:immunology; D001772:Blood Cell Count; D002374:Catalase / Q000378:metabolism; D002397:Catfishes / Q000097:blood / Q000276:immunology* / Q000469:parasitology; D016770:Ciliophora Infections / Q000097:blood / Q000276:immunology* / Q000469:parasitology / Q000662:veterinary; D005393:Fish Diseases / Q000097:blood / Q000276:immunology* / Q000469:parasitology; D005880:Gills / Q000469:parasitology; D006790:Host-Parasite Interactions; D016803:Hymenostomatida; D008099:Liver / Q000276:immunology / Q000378:metabolism; D016052:Protozoan Vaccines; D014611:Vaccination / Q000662:veterinary
chemical_list: D000913:Antibodies, Protozoan; D016052:Protozoan Vaccines; D002374:Catalase
keywords: Catalase; Ciliophora; Fish; Hematology; Immunoglobulin; Theronts; Vaccine; White spot disease