title: Biocompatible Polymeric Nanoparticles as Promising Candidates for Drug Delivery.
abstract: The use of polymeric nanoparticles (NPs) in pharmacology provides many benefits because this approach can increase the efficacy and selectivity of active compounds. However, development of new nanocarriers requires better understanding of the interactions between NPs and the immune system, allowing for the optimization of NP properties for effective drug delivery. Therefore, in the present study, we focused on the investigation of the interactions between biocompatible polymeric NPs and a murine macrophage cell line (RAW 264.7) and a human monocytic leukemia cell line (THP-1). NPs based on a liquid core with polyelectrolyte shells were prepared by sequential adsorption of polyelectrolytes (LbL) using AOT (docusate sodium salt) as the emulsifier and the biocompatible polyelectrolytes polyanion PGA (poly-l-glutamic acid sodium salt) and polycation PLL (poly l-lysine). The average size of the obtained NPs was 80 nm. Pegylated external layers were prepared using PGA-g-PEG (PGA grafted by PEG poly(ethylene glycol)). The influence of the physicochemical properties of the NPs (charge, size, surface modification) on viability, phagocytosis potential, and endocytosis was studied. Internalization of NPs was determined by flow cytometry and confocal microscopy. Moreover, we evaluated whether addition of PEG chains downregulates particle uptake by phagocytic cells. The presented results confirm that the obtained PEG-grafted NPs are promising candidates for drug delivery.
authors.affiliation: †Department of Physical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Krakow, Poland.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Łukasiewicz
authors.affiliation: ‡Jerzy Haber Institute of Catalysis and Surface Chemistry, PAS, 30-239 Krakow, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Szczepanowicz
authors.affiliation: †Department of Physical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Krakow, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Błasiak
authors.affiliation: †Department of Physical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Krakow, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Dziedzicka-Wasylewska
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D004143:Dioctyl Sulfosuccinic Acid / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry / Q000494:pharmacology; D045703:Emulsifying Agents / Q000737:chemistry*; D004705:Endocytosis / Q000187:drug effects; D006801:Humans; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D051379:Mice; D009000:Monocytes / Q000166:cytology / Q000187:drug effects; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D010587:Phagocytosis / Q000187:drug effects; D011092:Polyethylene Glycols / Q000737:chemistry; D011099:Polyglutamic Acid / Q000737:chemistry*; D011107:Polylysine / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D004337:Drug Carriers; D045703:Emulsifying Agents; D004143:Dioctyl Sulfosuccinic Acid; D011107:Polylysine; D011099:Polyglutamic Acid; D011092:Polyethylene Glycols