title: A novel cell-containing device for regenerative medicine: biodegradable nonwoven filters with peripheral blood cells promote wound healing.
abstract: The efficacy of skin regeneration devices consisting of nonwoven filters and peripheral blood cells was investigated for wound healing. We previously found that human peripheral blood cells enhanced their production of growth factors, such as transforming growth factor β1 (TGF-β1) and vascular endothelial growth factor, when they were captured on nonwoven filters. Cells on biodegradable filters were expected to serve as a local supply of growth factors and cell sources when they were placed in wounded skin. Nonwoven filters made of biodegradable polylactic acid (PLA) were cut out as 13-mm disks and placed into cell-capturing devices. Mouse peripheral blood was filtered, resulting in PLA filters with mouse peripheral blood cells (m-PBCs) at capture rates of 65.8 ± 5.2%. Then, the filters were attached to full-thickness surgical wounds in a diabetic db/db mouse skin for 14 days as a model of severe chronic wounds. The wound area treated with PLA nonwoven filters with m-PBCs (PLA/B+) was reduced to 8.5 ± 12.2% when compared with day 0, although the non-treated control wounds showed reduction only to 60.6 ± 27.8%. However, the PLA filters without m-PBCs increased the wound area to 162.9 ± 118.7%. By histopathological study, the PLA/B+ groups more effectively accelerated formation of epithelium. The m-PBCs captured on the PLA filters enhanced keratinocyte growth factor (FGF-7) and TGF-β1 productions in vitro, which may be related to wound healing. This device is useful for regeneration of wounded skin and may be adaptable for another application.
authors.affiliation: Healthcare R&D Center, Asahi Kasei Corporation, Tokyo, Japan.
authors.forename: Ushio
authors.initials: U
authors.lastname: Iwamoto
authors.affiliation: Faculty of Clinical Engineering, School of Health Sciences, Fujita Health University, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake, Aichi, 470-1192, Japan. hori@fujita-hu.ac.jp.
authors.forename: Hideo
authors.initials: H
authors.lastname: Hori
authors.affiliation: Department of Plastic Surgery, School of Medicine, Kyorin University, Tokyo, Japan.|Japan Labour Health and Welfare Organization, Tokyo Rosai Hospital, Tokyo, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Takami
authors.affiliation: Therapeutic Apheresis Division, Asahi Kasei Medical, Co., Ltd, Tokyo, Japan.
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Tokushima
authors.affiliation: Faculty of Medical Management and Information Science, School of Health Sciences, Fujita Health University, Toyoake, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Shinzato
authors.affiliation: Healthcare R&D Center, Asahi Kasei Corporation, Tokyo, Japan.
authors.forename: Mikitomo
authors.initials: M
authors.lastname: Yasutake
authors.affiliation: Faculty of Clinical Engineering, School of Health Sciences, Fujita Health University, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake, Aichi, 470-1192, Japan. nkitaguc@fujita-hu.ac.jp.
authors.forename: Nobuya
authors.initials: N
authors.lastname: Kitaguchi
mesh_terms: D000818:Animals; D001773:Blood Cells / Q000502:physiology*; D019344:Lactic Acid; D051379:Mice; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D011091:Polyesters; D011108:Polymers; D044968:Regenerative Medicine / Q000295:instrumentation*; D012883:Skin Ulcer / Q000209:etiology / Q000473:pathology / Q000628:therapy*; D023822:Tissue Engineering / Q000295:instrumentation*; D054457:Tissue Scaffolds; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D014945:Wound Healing / Q000502:physiology*
chemical_list: D011091:Polyesters; D011108:Polymers; D053773:Transforming Growth Factor beta1; D042461:Vascular Endothelial Growth Factor A; D019344:Lactic Acid; C033616:poly(lactide)
keywords: FGF-7; Nonwoven filter; Peripheral blood cells; TGF-β1; Wound healing