title: Visible Thrombolysis Acceleration of a Nanomachine Powered by Light-Driving F0F1-ATPase Motor.
abstract: We report on thrombolysis acceleration of a nanomachine powered by light-driving δ-subunit-free F0F1-ATPase motor. It is composed of a mechanical device, locating device, energy storage device, and propeller. The rotory δ-subunit-free F0F1-ATPase motor acts as a mechanical device, which was obtained by reconstructing an original chromatophore extracted from Rhodospirillum rubrum. We found that the bioactivity of the F0F1-ATPase motor improved greatly after reconstruction. The zeta potential of the nanomachine is about -23.4 mV. Cytotoxicity induced by the nanomachine was measured using cell counting kit (CCK)-8 assay. The A549 cells incubated with different fractional concentrations of the nanomachine within 48 h did not show obvious cytotoxicity. The locating device helps the nanomachine bind to the thrombi. Energy was easily stored by exposing the nanomachine to 600-nm-wavelength irradiation, which promoted activity of the motor. The rotation of the long propeller accelerated thrombolysis of a blood clot in vitro in the presence of urokinase (UK). This result was based on visual inspection and confirmed by a series of tests.
authors.affiliation: The National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101 China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Duan
authors.affiliation: Department of Interventional Neuroradiology, Beijing Tiantan Hospital, Capital Medical University, 6 Tiantan Xili, Beijing, 100050 China ; Department of Neurology, Liaocheng People's Hospital and Liaocheng Clinical School of Taishan Medical University, Liaocheng, Shandong 252000 China.
authors.forename: Lifeng
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Interventional Neuroradiology, Beijing Tiantan Hospital, Capital Medical University, 6 Tiantan Xili, Beijing, 100050 China ; New Era Stroke Care and Research Institute of The Second Artillery General Hospital PLA, 16 Xinjiekouwai Avenue, Beijing, 100088 China.
authors.forename: Weijian
authors.initials: W
authors.lastname: Jiang
authors.affiliation: The National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101 China.
authors.forename: Jiachang
authors.initials: J
authors.lastname: Yue
keywords: F0F1-ATPase; Nanomachine; Thrombolysis; Urokinase