title: Study on the interactions between anti-cancer drug oxaliplatin and DNA by atomic force microscopy.
abstract: Oxaliplatin is one of the most important anticancer drugs at present. However, the mechanism of action of oxaliplatin is still controversial. In this study, the interactions between oxaliplatin and a plasmid DNA have been studied so as to reveal the structural basis of its activity. The structural characteristic of pUC19 DNA (2ng/μL) incubated with 100μmol/L and 1000μmol/L of oxaliplatin for the different time on a freshly cleaved highly ordered pyrolytic graphite (HOPG) surface was investigated by atomic force microscopy (AFM). High resolution AFM observation indicated that oxaliplatin can induce pUC19 DNA molecules change from the extended conformation to the entangled structures with many nodes, and finally to the compact particles. The present AFM results provide structural evidence about the interactions between oxaliplatin and circular duplex DNA containing multiple targets.
authors.affiliation: Institute of higher education, Northeast Forestry University, Harbin 150040, People's Republic of China.
authors.forename: Leipeng
authors.initials: L
authors.lastname: Li
authors.affiliation: State Engineering Laboratory of Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, People's Republic of China; Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, People's Republic of China.
authors.forename: Ruisi
authors.initials: R
authors.lastname: Liu
authors.affiliation: State Engineering Laboratory of Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, People's Republic of China; Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, People's Republic of China.
authors.forename: Fengjie
authors.initials: F
authors.lastname: Xu
authors.affiliation: State Engineering Laboratory of Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, People's Republic of China; Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, People's Republic of China.
authors.forename: Yuangang
authors.initials: Y
authors.lastname: Zu
authors.affiliation: State Engineering Laboratory of Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, People's Republic of China; Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, People's Republic of China. Electronic address: cn.zguoliu@yahoo.com.
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000970:Antineoplastic Agents / Q000378:metabolism*; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D018625:Microscopy, Atomic Force; D009690:Nucleic Acid Conformation / Q000187:drug effects*; D009944:Organoplatinum Compounds / Q000378:metabolism*; D000077150:Oxaliplatin; D010957:Plasmids
chemical_list: D000970:Antineoplastic Agents; D009944:Organoplatinum Compounds; D000077150:Oxaliplatin; D004247:DNA
keywords: AFM; Atomic force microscopy; DNA; HOPG; Oxaliplatin