title: Protective effects of traditional Chinese medicine formula NaoShuanTong capsule on haemorheology and cerebral energy metabolism disorders in rats with blood stasis.
abstract: NaoShuanTong capsule (NSTC), an oral traditional Chinese medicine formula, is composed ofPollen Typhae,Radix Paeoniae Rubra,Rhizoma Gastrodiae,Radix RhaponticiandRadix Curcumae. It has been widely used to treat ischemic stroke in clinic for many years in China. In addition to neuronal apoptosis, haemorheology and cerebral energy metabolism disorders also play an important role in the pathogenesis and development of ischemic stroke. The present study was designed to evaluate thein vivoprotective effects of NSTC on haemorheology and cerebral energy metabolism disorders in rats with blood stasis. Sixty specific pathogen-free sprague-dawley rats, male only, were randomly divided into six groups (control group, model group, aspirin (100 mg/kg/d) group, NSTC low-dose (400 mg/kg/d) group, NSTC intermediate-dose (800 mg/kg/d) group, NSTC high-dose (1600 mg/kg/d) group) with 10 animals in each. The rats except those in the control group were placed in ice-cold water (0-4 °C) for 5 min during the time interval (4 h) of two adrenaline hydrochloride injections (0.8 mg/kg) to induce blood stasis. After treatment, whole blood viscosity at three shear rates, plasma viscosity and erythrocyte sedimentation rate significantly decreased in NSTC intermediate- and high-dose groups; erythrocyte aggregation index and red corpuscle electrophoresis index significantly decreased in all the three dose NSTC groups. Moreover, treatment with high-dose NSTC could significantly improve Na+-K+adenosine triphosphatase (ATPase) and Ca2+ATPase activity, as well as lower lactic acid level in brain tissues. These results demonstrated the protective effects of NSTC on haemorheology and cerebral energy metabolism disorders, which may provide scientific information for the further understanding of mechanism(s) of NSTC as a clinical treatment for ischemic stroke. Furthermore, the protective effects of activating blood circulation as observed in this study might create valuable insight for the utilisation of NSTC to be a feasible alternative therapeutic agent for patients with blood stasis.
authors.affiliation: Guangzhou Quality R & D Center of Traditional Chinese Medicine, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University , Guangzhou , P.R. China ; cGuangzhou Blood Center , Guangzhou , P.R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.affiliation: Guangzhou Quality R & D Center of Traditional Chinese Medicine, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University , Guangzhou , P.R. China ; cGuangzhou Blood Center , Guangzhou , P.R. China.
authors.forename: Yao-Yao
authors.initials: YY
authors.lastname: Peng
authors.affiliation: Guangzhou Quality R & D Center of Traditional Chinese Medicine, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University , Guangzhou , P.R. China.
authors.forename: Feng-Yin
authors.initials: FY
authors.lastname: Liang
authors.affiliation: Guangzhou Quality R & D Center of Traditional Chinese Medicine, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University , Guangzhou , P.R. China.
authors.forename: Si
authors.initials: S
authors.lastname: Chen
authors.affiliation: Guangzhou Quality R & D Center of Traditional Chinese Medicine, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University , Guangzhou , P.R. China.
authors.forename: Pei-Bo
authors.initials: PB
authors.lastname: Li
authors.affiliation: Guangzhou Quality R & D Center of Traditional Chinese Medicine, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University , Guangzhou , P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Peng
authors.affiliation: Guangzhou Quality R & D Center of Traditional Chinese Medicine, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University , Guangzhou , P.R. China.
authors.forename: Zhong-Zheng
authors.initials: ZZ
authors.lastname: Liu
authors.affiliation: Guangdong Zhongsheng Pharmaceutical Co. Ltd. , Dongguan , P.R. China.
authors.forename: Cheng-Shi
authors.initials: CS
authors.lastname: Xie
authors.affiliation: Guangdong Zhongsheng Pharmaceutical Co. Ltd. , Dongguan , P.R. China.
authors.forename: Chao-Feng
authors.initials: CF
authors.lastname: Long
authors.affiliation: Guangzhou Quality R & D Center of Traditional Chinese Medicine, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University , Guangzhou , P.R. China.
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Su
keywords: Chinese medicine formula; NaoShuanTong capsule; blood stasis; cerebral energy metabolism; haemorheology