title: Evaluation of follicular synchronization caused by estrogen administration and its reproductive outcome.
abstract: To evaluate multiple follicular development synchronization after estrogen stimulation in prepubertal mice, follicular responsiveness to gonadotropin superovulation, the prospective reproductive potential and ovarian polycystic ovary syndrome (PCOS)-like symptoms at adulthood, prepubertal mice were intraperitoneally injected with estrogen to establish an animal model with solvent as control. When synchronized tertiary follicles in ovaries, in vitro oocyte maturation and fertilization rates, blastocyst formation rate, developmental potential into offspring by embryo transfer, adult fertility and PCOS-like symptoms, and involved molecular mechanisms were focused, it was found that estrogen stimulation (10 μg/gBW) leads to follicular development synchronization at the early tertiary stage in prepubertal mice; reproduction from oocytes to offspring could be realized by means of the artificial reproductive technology though the model mice lost their natural fertility when they were reared to adulthood; and typical symptoms of PCOS, except changes in inflammatory pathways, were not remained up to adulthood. So in conclusion, estrogen can lead to synchronization in follicular development in prepubertal mice, but does not affect reproductive outcome of oocytes, and no typical symptoms of PCOS remained at adulthood despite changes related to inflammation.
authors.affiliation: Shanghai Medical College, Institute of Reproduction and Development, Fudan University, Shanghai, P. R. China.
authors.forename: Bi
authors.initials: B
authors.lastname: Wu
authors.affiliation: Key Laboratory of Contraceptive Drugs and Devices of National Population and Family Planning Commission of China, Shanghai Institute of Planned Parenthood Research, Shanghai, P. R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Department of Food Science, Shanghai Business School, Shanghai, P. R. China.
authors.forename: Xia
authors.initials: X
authors.lastname: Gong
authors.affiliation: Key Laboratory of Contraceptive Drugs and Devices of National Population and Family Planning Commission of China, Shanghai Institute of Planned Parenthood Research, Shanghai, P. R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Yu
authors.affiliation: Department of Assisted Reproduction, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, P. R. China.
authors.forename: Qiuju
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Key Laboratory of Contraceptive Drugs and Devices of National Population and Family Planning Commission of China, Shanghai Institute of Planned Parenthood Research, Shanghai, P. R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Contraceptive Drugs and Devices of National Population and Family Planning Commission of China, Shanghai Institute of Planned Parenthood Research, Shanghai, P. R. China.
authors.forename: Zhaogui
authors.initials: Z
authors.lastname: Sun
mesh_terms: D000818:Animals; D004958:Estradiol / Q000031:analogs & derivatives* / Q000097:blood / Q000494:pharmacology; D005260:Female; D005298:Fertility / Q000187:drug effects*; D007328:Insulin / Q000097:blood; D020738:Leptin / Q000097:blood; D051379:Mice; D006080:Ovarian Follicle / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D010053:Ovary / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D010062:Ovulation Induction; D011247:Pregnancy; D018873:Pregnancy Rate; D011374:Progesterone / Q000097:blood; D013480:Superovulation / Q000187:drug effects*; D013739:Testosterone / Q000097:blood
chemical_list: D007328:Insulin; D020738:Leptin; C074283:estradiol 3-benzoate; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol