title: Effect of neonatal asphyxia on the impairment of the auditory pathway by recording auditory brainstem responses in newborn piglets: a new experimentation model to study the perinatal hypoxic-ischemic damage on the auditory system.
abstract: BACKGROUND
  Hypoxia-ischemia (HI) is a major perinatal problem that results in severe damage to the brain impairing the normal development of the auditory system. The purpose of the present study is to study the effect of perinatal asphyxia on the auditory pathway by recording auditory brain responses in a novel animal experimentation model in newborn piglets.
  
  
  METHODS
  Hypoxia-ischemia was induced to 1.3 day-old piglets by clamping 30 minutes both carotid arteries by vascular occluders and lowering the fraction of inspired oxygen. We compared the Auditory Brain Responses (ABRs) of newborn piglets exposed to acute hypoxia/ischemia (n = 6) and a control group with no such exposure (n = 10). ABRs were recorded for both ears before the start of the experiment (baseline), after 30 minutes of HI injury, and every 30 minutes during 6 h after the HI injury.
  
  
  RESULTS
  Auditory brain responses were altered during the hypoxic-ischemic insult but recovered 30-60 minutes later. Hypoxia/ischemia seemed to induce auditory functional damage by increasing I-V latencies and decreasing wave I, III and V amplitudes, although differences were not significant.
  
  
  CONCLUSIONS
  The described experimental model of hypoxia-ischemia in newborn piglets may be useful for studying the effect of perinatal asphyxia on the impairment of the auditory pathway.
authors.affiliation: Research Unit on Experimental Perinatal Physiopathology, Cruces University Hospital, Barakaldo, 48080, Bizkaia, Spain.
authors.forename: Francisco Jose
authors.initials: FJ
authors.lastname: Alvarez
authors.affiliation: Department of Cell Biology and Histology, Faculty of Medicine and Dentistry, University of the Basque Country, Barrio Sarriena s/n, Leioa, 48940, Bizkaia, Spain.
authors.forename: Miren
authors.initials: M
authors.lastname: Revuelta
authors.affiliation: Department of Otorhinolaryngology, Basurto University Hospital, Faculty of Medicine, University of the Basque Country, Barrio Sarriena s/n, Leioa, 48940, Bizkaia, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Santaolalla
authors.affiliation: Department of Cell Biology and Histology, Faculty of Medicine and Dentistry, University of the Basque Country, Barrio Sarriena s/n, Leioa, 48940, Bizkaia, Spain.
authors.forename: Antonia
authors.initials: A
authors.lastname: Alvarez
authors.affiliation: Research Unit on Experimental Perinatal Physiopathology, Cruces University Hospital, Barakaldo, 48080, Bizkaia, Spain.
authors.forename: Hector
authors.initials: H
authors.lastname: Lafuente
authors.affiliation: Department of Cell Biology and Histology, Faculty of Medicine and Dentistry, University of the Basque Country, Barrio Sarriena s/n, Leioa, 48940, Bizkaia, Spain.
authors.forename: Olatz
authors.initials: O
authors.lastname: Arteaga
authors.affiliation: Department of Cell Biology and Histology, Faculty of Medicine and Dentistry, University of the Basque Country, Barrio Sarriena s/n, Leioa, 48940, Bizkaia, Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: Alonso-Alconada
authors.affiliation: Department of Otorhinolaryngology, Basurto University Hospital, Faculty of Medicine, University of the Basque Country, Barrio Sarriena s/n, Leioa, 48940, Bizkaia, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Sanchez-del-Rey
authors.affiliation: Department of Cell Biology and Histology, Faculty of Medicine and Dentistry, University of the Basque Country, Barrio Sarriena s/n, Leioa, 48940, Bizkaia, Spain.
authors.forename: Enrique
authors.initials: E
authors.lastname: Hilario
authors.affiliation: Department of Otorhinolaryngology, Basurto University Hospital, Faculty of Medicine, University of the Basque Country, Barrio Sarriena s/n, Leioa, 48940, Bizkaia, Spain.
authors.forename: Agustin
authors.initials: A
authors.lastname: Martinez-Ibargüen
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001237:Asphyxia / Q000503:physiopathology*; D001306:Auditory Pathways / Q000503:physiopathology*; D004195:Disease Models, Animal; D016057:Evoked Potentials, Auditory, Brain Stem; D006801:Humans; D020925:Hypoxia-Ischemia, Brain / Q000503:physiopathology*; D011930:Reaction Time