title: Biotransformation of steroidal saponins in sisal (Agave sisalanaPerrine) to tigogenin by a newly isolated strain from a karst area of Guilin, China.
abstract: A rod-shaped bacterium was isolated from the soil in a karst area of Guilin, China and its biotransformation of steroidal saponins in sisal (Agave sisalanaPerrine) to tigogenin was presented for the first time. A total of 22 strains for the degradation of steroidal saponins in sisal were isolated from 48 soil samples, and the isolated rod-shaped, bacterial strain ZG-21 was used for the production of tigogenin due to its highest degradation efficiency of steroidal saponins in sisal. The parameters affecting biotransformation by strain ZG-21 were optimized. Under the optimized conditions of temperature (30 °C), pH (6), time (5 days) and substrate concentration (5 mg/mL), a maximum tigogenin yield of 26.7 mg/g was achieved. Compared with the conventional method of acid hydrolysis, the biotransformation method provided a clean and eco-friendly alternative for the production of tigogenin.
authors.affiliation: College of Chemistry and Bioengineering, Guilin University of Technology , Guilin , China ; College of Life Science, Northeast Agriculture University , Harbin , China.
authors.forename: Yanchao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Chemistry and Bioengineering, Guilin University of Technology , Guilin , China.
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Chemistry and Bioengineering, Guilin University of Technology , Guilin , China.
authors.forename: Hao
authors.initials: H
authors.lastname: Sun
authors.affiliation: Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences , Haikou , China.
authors.forename: Kexian
authors.initials: K
authors.lastname: Yi
authors.affiliation: Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences , Haikou , China.
authors.forename: Jinlong
authors.initials: J
authors.lastname: Zheng
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences , Beijing , China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Bioengineering, Guilin University of Technology , Guilin , China ; College of Life Science, Northeast Agriculture University , Harbin , China.
authors.forename: Zaibin
authors.initials: Z
authors.lastname: Hao
keywords: acid hydrolysis; biotransformation; sisal; steroidal saponins; tigogenin