title: Low Voltage Transmission Electron Microscopy in Cell Biology.
abstract: Low voltage transmission electron microscopy (LVTEM) was employed to examine biological tissues with accelerating voltages as low as 5kV. Tissue preparation was modified to take advantage of the low-voltage techniques. Treatments with heavy metals, such as post-fixation with osmium tetroxide, on block and counterstaining were omitted. Sections (40nm) were thinner than usual and generated highly contrasted images. General appearance of the cells remains similar to that of conventional TEM. New features were however revealed. The matrix of the pancreatic granules displays heterogeneity with partitions that may correspond to the inner-segregation of their secretory proteins. Mitochondria revealed the presence of the ATP synthase granules along their cristea. The nuclear dense chromatin displayed a honeycomb organization while distinct beads, nucleosomes, aligned along thin threads were seen in the dispersed chromatin. Nuclear pore protein complexes revealed their globular nature. The intercalated disks in cardiac muscle displayed their fine structural organization. These features correlate well with data described or predicted by cell and molecular biology. These new aspects are not revealed when thicker and conventionally osmicated tissue sections were examined by LVTEM, indicating that major masking effects are associated with standard TEM techniques. Immunogold was adapted to LVTEM further enhancing its potential in cell biology.
authors.affiliation: Department of Pathology and Cell Biology, University of Montreal. Electronic address: moise.bendayan@umontreal.ca.
authors.forename: Moise
authors.initials: M
authors.lastname: Bendayan
authors.affiliation: Delong America Inc., Montreal, Quebec, Canada.
authors.forename: Eugene
authors.initials: E
authors.lastname: Paransky
mesh_terms: D000681:Amylases / Q000032:analysis / Q000378:metabolism; D000818:Animals; D007150:Immunohistochemistry / Q000379:methods; D034904:Lamin Type A / Q000032:analysis / Q000378:metabolism; D046529:Microscopy, Electron, Transmission / Q000295:instrumentation / Q000379:methods*; D008867:Microtomy / Q000379:methods*; D008928:Mitochondria / Q000648:ultrastructure; D009206:Myocardium / Q000378:metabolism / Q000648:ultrastructure*; D028861:Nuclear Pore Complex Proteins / Q000648:ultrastructure; D009707:Nucleosomes / Q000648:ultrastructure; D010179:Pancreas / Q000378:metabolism / Q000648:ultrastructure*; D051381:Rats; D017207:Rats, Sprague-Dawley; D022142:Secretory Vesicles / Q000648:ultrastructure; D016610:Tissue Embedding / Q000379:methods; D016707:Tissue Fixation / Q000379:methods
chemical_list: D034904:Lamin Type A; D028861:Nuclear Pore Complex Proteins; D009707:Nucleosomes; D000681:Amylases
keywords: Cardiac muscle; Immunogold; Lamins; Low voltage transmission electron microscopy; Mitochondria; Nucleus; Pancreas; Secretory granules