title: Defined nanoscale chemistry influences delivery of peptido-toxins for cancer therapy.
abstract: We present an in-silico-to-in-vitro approach to develop well-defined, self-assembled, rigid-cored polymeric (Polybee) nano-architecture for controlled delivery of a key component of bee venom, melittin. A competitive formulation with lipid-encapsulated (Lipobee) rigid cored micelle is also synthesized. In a series of sequential experiments, we show how nanoscale chemistry influences the delivery of venom toxins for cancer regression and help evade systemic disintegrity and cellular noxiousness. A relatively weaker association of melittin in the case of lipid-based nanoparticles is compared to the polymeric particles revealed by energy minimization and docking studies, which are supported by biophysical studies. For the first time, the authors' experiment results indicate that melittin can play a significant role in DNA association-dissociation processes, which may be a plausible route for their anticancer activity.
authors.affiliation: Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, United States of America; Beckman Institute of Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, United States of America; Department of Materials Science and Engineering University of Illinois at Urbana-Champaign, Urbana, IL, 61801, United States of America; Carle Foundation Hospital, Urbana, IL, 61801, United States of America.
authors.forename: Santosh K
authors.initials: SK
authors.lastname: Misra
authors.affiliation: Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, United States of America; Beckman Institute of Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, United States of America; Department of Materials Science and Engineering University of Illinois at Urbana-Champaign, Urbana, IL, 61801, United States of America; Carle Foundation Hospital, Urbana, IL, 61801, United States of America.
authors.forename: Mao
authors.initials: M
authors.lastname: Ye
authors.affiliation: Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, United States of America; Beckman Institute of Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, United States of America; Department of Materials Science and Engineering University of Illinois at Urbana-Champaign, Urbana, IL, 61801, United States of America; Carle Foundation Hospital, Urbana, IL, 61801, United States of America.
authors.forename: Sumin
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, United States of America; Beckman Institute of Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, United States of America; Department of Materials Science and Engineering University of Illinois at Urbana-Champaign, Urbana, IL, 61801, United States of America; Carle Foundation Hospital, Urbana, IL, 61801, United States of America.
authors.forename: Dipanjan
authors.initials: D
authors.lastname: Pan
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry; D008555:Melitten / Q000737:chemistry*; D062105:Molecular Docking Simulation; D053758:Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D017433:Protein Structure, Secondary
chemical_list: D000970:Antineoplastic Agents; D011108:Polymers; D008555:Melitten