title: High density of tryptase-positive mast cells in patients with multiple myeloma: correlation with parameters of disease activity.
abstract: Multiple myeloma (MM) is a plasma cell neoplasm characterized by bone marrow infiltration from malignant plasma cells. Mast cells play an important role in inflammation and angiogenesis in malignant diseases. The aim of the study was to evaluate the mast cell density in bone marrow of untreated MM patients with markers of disease activity such as serum interleukin-6 (IL-6), B2M, and C-reactive protein (CRP), the grade of bone marrow infiltration, and the levels of produced paraprotein. We studied 86 newly diagnosed MM patients (46 males, 40 females, mean age 59 ± 13.7 years). Thirty of them reached plateau phase after chemotherapy and 20 healthy volunteers. According to the criteria of International Staging System (ISS) staging system, 23 patients had stage I, 30 had stage II, and 33 had stage III. The serum concentrations of CRP, B2M, and IL-6, and the mast cell density (MCD) values were significantly higher in MM patients' group (1.6 ± 1.8, 4.3 ± 2.9, 7.1 ± 5.1, and 9 ± 4.8), in comparison with those found in control group (0.4 ± 0.1, 1.5 ± 0.6, 1.1 ± 0.5, and 1.9 ± 0.7; p < 0.001 in all the cases). Significant differences were found between the grade of infiltration in bone marrow, and the paraprotein values in patients' serum before and after chemotherapy. Furthermore, there was a significant correlation between the MCD values and the prognostic markers CRP (r = 0.452, p < 0.0001), IL-6 (r = 0.475, p < 0.0001), bone marrow infiltration (r = 0.333, p < 0.0002), and serum paraprotein levels(r = 0.221, p < 0.04). High MCD values strengthen the hypothesis that mast cells participate in the pathogenesis of disease progression and may be used as an indicator of the disease activity.
authors.affiliation: Department of Heamatology, University Hospital of Heraklion, PO Box 1352, 71110, Heraklion, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Devetzoglou
authors.affiliation: Laboratory of Heamatology, University Hospital of Heraklion, Heraklion, Greece.
authors.forename: Rodanthi
authors.initials: R
authors.lastname: Vyzoukaki
authors.affiliation: Laboratory of Heamatology, University Hospital of Heraklion, Heraklion, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Kokonozaki
authors.affiliation: Department of Pathology, Venizeleion General Hospital of Heraklion, Heraklion, Greece.
authors.forename: Athina
authors.initials: A
authors.lastname: Xekalou
authors.affiliation: Department of Internal Medicine, Venizeleion General Hospital of Heraklion, Heraklion, Greece.
authors.forename: Constantina A
authors.initials: CA
authors.lastname: Pappa
authors.affiliation: Laboratory of Heamatology, University Hospital of Heraklion, Heraklion, Greece.
authors.forename: Anastasia
authors.initials: A
authors.lastname: Papadopoulou
authors.affiliation: School of Medicine, University of Crete, Heraklion, Greece.
authors.forename: Athanasios
authors.initials: A
authors.lastname: Alegakis
authors.affiliation: Laboratory of Heamatology, University Hospital of Heraklion, Heraklion, Greece.
authors.forename: Nikolaos
authors.initials: N
authors.lastname: Androulakis
authors.affiliation: Department of Heamatology, University Hospital of Heraklion, PO Box 1352, 71110, Heraklion, Greece. alexandm@med.uoc.gr.|Laboratory of Heamatology, University Hospital of Heraklion, Heraklion, Greece. alexandm@med.uoc.gr.
authors.forename: Michael G
authors.initials: MG
authors.lastname: Alexandrakis
mesh_terms: D000328:Adult; D000368:Aged; D001853:Bone Marrow / Q000473:pathology; D002097:C-Reactive Protein / Q000378:metabolism; D002452:Cell Count; D005260:Female; D006801:Humans; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D008407:Mast Cells / Q000201:enzymology / Q000473:pathology; D008875:Middle Aged; D009101:Multiple Myeloma / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D009367:Neoplasm Staging; D009389:Neovascularization, Pathologic / Q000235:genetics* / Q000473:pathology; D011379:Prognosis; D053802:Tryptases / Q000096:biosynthesis / Q000235:genetics*
chemical_list: C508600:IL6 protein, human; D015850:Interleukin-6; D002097:C-Reactive Protein; D053802:Tryptases
keywords: Acute phase proteins; Angiogenesis; Mast cells; Multiple myeloma; Prognosis