title: The longevity effect of echinacoside in Caenorhabditis elegans mediated through daf-16.
abstract: Echinacoside (ECH), a natural polyphenolic compound, has been reported to possess important pharmacological activities. However, very little is known about whether or how ECH affects longevity in vivo. We have examined the effects of ECH on the life span and stress tolerance in Caenorhabditis elegans. Our studies demonstrate that the life span of wild-type worms could be extended in the presence of ECH. Furthermore, ECH was found to increase tolerance of worms to heat shock and oxidative stress, while not exerting any influence on pharyngeal pumping rate and progeny production. Our mechanistic studies indicate that supplementation of ECH increases the transcript level of daf-16. ECH treatment also modulates the nuclear localization and transcriptional activities of daf-16, thus fine tunes the expression of daf-16 target genes to promote longevity and increases stress response in C. elegans. Overall, this work reveals the longevity effect of ECH and elucidates the underpinning mechanisms.
authors.affiliation: a Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Science and Technology , Tianjin University , Tianjin , China.
authors.forename: Xue
authors.initials: X
authors.lastname: Wang
authors.forename: Jiaolong
authors.initials: J
authors.lastname: Zhang
authors.forename: Lulu
authors.initials: L
authors.lastname: Lu
authors.forename: Lijun
authors.initials: L
authors.lastname: Zhou
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017173:Caenorhabditis elegans / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000819:agonists* / Q000235:genetics / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D051858:Forkhead Transcription Factors / Q000819:agonists* / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D006027:Glycosides / Q000494:pharmacology*; D018869:Heat-Shock Response / Q000187:drug effects; D006358:Hot Temperature; D007814:Larva / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D008136:Longevity / Q000187:drug effects* / Q000235:genetics; D015394:Molecular Structure; D018384:Oxidative Stress / Q000187:drug effects; D010269:Paraquat / Q000037:antagonists & inhibitors / Q000494:pharmacology; D020011:Protective Agents / Q000494:pharmacology*; D012333:RNA, Messenger / Q000819:agonists* / Q000235:genetics / Q000378:metabolism; D012098:Reproduction; D014158:Transcription, Genetic
chemical_list: D000975:Antioxidants; D029742:Caenorhabditis elegans Proteins; D051858:Forkhead Transcription Factors; D006027:Glycosides; D020011:Protective Agents; D012333:RNA, Messenger; C108831:daf-16 protein, C elegans; C060297:echinacoside; D010269:Paraquat
keywords: Caenorhabditis elegans; daf-16; echinacoside; longevity