title: Rapid identification ofPseudomonas aeruginosaby pulsed-field gel electrophoresis.
abstract: Twenty clinicalPseudomonas aeruginosaisolates recovered from patients admitted to The General Hospital in Ismailia Governorate (Egypt) were examined in this study. We analysedP. aeruginosaATCC 9027 (as a control strain) and 19 of the isolates after digestion withSpeIrestriction endonuclease. After this we conducted a pulsed-field gel electrophoresis (PFGE) and typed the obtained 10 unique patterns, designated as A, A1, B, B1, C, C1, D, D1, E and F. We evaluated the genetic relatedness between all strains, based on ≥87% band identity. As a result, the isolates were grouped in the 10 clusters as follows: patterns A, A1, B, B1, C contained two strains each and patterns C1, D, D1, E contained a single strain each; the five remaining strains were closely related (genomic pattern F). One isolate belonged to antibiotype 'b'. The genotype patterns of theP. aeruginosaATCC 9027 control strain and isolate no. 11 were closely related and had two different antibiotypes 'd' and 'c', respectively.
authors.affiliation: Department of Clinical Laboratory Sciences, College of Applied Medical Science, Aljouf University , Sakaka , Saudi Arabia ; Department of Botany, Faculty of Science, Suez Canal University , Ismailia , Egypt.
authors.forename: Samy
authors.initials: S
authors.lastname: Selim
authors.affiliation: Clinical Pathology Department, Ain Shams University Specialized Hospital, Ain Shams University , Cairo , Egypt.
authors.forename: Iman
authors.initials: I
authors.lastname: El Kholy
authors.affiliation: Department of Botany, Faculty of Science, Suez Canal University , Ismailia , Egypt.
authors.forename: Nashwa
authors.initials: N
authors.lastname: Hagagy
authors.affiliation: Department of Botany, Faculty of Science, Suez Canal University , Ismailia , Egypt.
authors.forename: Sahar
authors.initials: S
authors.lastname: El Alfay
authors.affiliation: Department of Botany, Faculty of Science, Suez Canal University , Ismailia , Egypt.
authors.forename: Mohamed Abdel
authors.initials: MA
authors.lastname: Aziz
keywords: Egypt; Pseudomonas aeruginosa; pulsed-field gel electrophoresis