title: [Lentivirus-mediated angiotensin-converting enzyme 2 overexpression inhibits angiotensin II-induced albumin down-regulation and enhancement of migration in rat hepatocytes].
abstract: OBJECTIVE
  To investigate the inhibitory effect of angiotensin-converting enzyme 2 (ACE2) on angiotensin II (Ang II)-induced down-regulation of albumin expression and enhancement of cell migration in rat hepatocytes.
  
  
  METHODS
  Cultured rat hepatocyte were treated with Ang II (10-7 mol/L) for different time lengths, and the protein expressions of vimentin and albumin and cell migration were detected. The cells transfected with lentiGFP or lentiACE2 were treated with A779 for 1 h and then with Ang II, and Western blotting and immunofluorescent cytochemistry were used to detect the protein levels; the cell migration was evaluated by Transwell assay.
  
  
  RESULTS
  Ang II induced significantly increased vimentin expression and reduced albumin expression in rat hepatocytes in a time-dependent manner. Overexpression of ACE2 obviously inhibited the up-regulation of vimentin expression, reduction of albumin expression, and enhancement of cell migration induced by Ang II.
  
  
  CONCLUSIONS
  ACE2 overexpression can inhibit Ang II-induced up-regulation of vimentin, reduction of albumin expression, and enhancement of cell migration in rat hepatocytes.
authors.affiliation: Department of Emergency Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.E-mail: lilyzhang4444@126.com.
authors.forename: Lili
authors.initials: L
authors.lastname: Zhang
authors.forename: Wenyong
authors.initials: W
authors.lastname: Zhang
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Xu
authors.initials: X
authors.lastname: Li
mesh_terms: D000418:Albumins / Q000378:metabolism*; D000804:Angiotensin II / Q000494:pharmacology*; D000085962:Angiotensin-Converting Enzyme 2; D000818:Animals; D002465:Cell Movement; D002478:Cells, Cultured; D015536:Down-Regulation; D022781:Hepatocytes / Q000166:cytology*; D016086:Lentivirus; D007703:Peptidyl-Dipeptidase A / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D014162:Transfection; D015854:Up-Regulation; D014746:Vimentin / Q000378:metabolism
chemical_list: D000418:Albumins; D014746:Vimentin; D000804:Angiotensin II; D007703:Peptidyl-Dipeptidase A; C000705329:Ace2 protein, rat; D000085962:Angiotensin-Converting Enzyme 2