title: Structural and physical properties of collagen extracted from moon jellyfish under neutral pH conditions.
abstract: We extracted collagen from moon jellyfish under neutral pH conditions and analyzed its amino acid composition, secondary structure, and thermal stability. The content of hydroxyproline was 4.3%, which is lower than that of other collagens. Secondary structure analysis using circular dichroism (CD) showed a typical collagen helix. The thermal stability of this collagen at pH 3.0 was lower than those from fish scale and pig skin, which also correlates closely with jellyfish collagen having lower hydroxyproline content. Because the solubility of jellyfish collagen used in this study at neutral pH was quite high, it was possible to analyze its structural and physical properties under physiological conditions. Thermodynamic analysis using CD and differential scanning calorimetry showed that the thermal stability at pH 7.5 was higher than at pH 3.0, possibly due to electrostatic interactions. During the process of unfolding, fibrillation would occur only at neutral pH.
authors.affiliation: a Graduate School of Life and Environmental Sciences , Kyoto Prefectural University , Kyoto , Japan.
authors.forename: Ayako
authors.initials: A
authors.lastname: Miki
authors.forename: Satomi
authors.initials: S
authors.lastname: Inaba
authors.forename: Takayuki
authors.initials: T
authors.lastname: Baba
authors.forename: Koji
authors.initials: K
authors.lastname: Kihira
authors.forename: Harumi
authors.initials: H
authors.lastname: Fukada
authors.forename: Masayuki
authors.initials: M
authors.lastname: Oda
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D003094:Collagen / Q000737:chemistry* / Q000302:isolation & purification; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D006909:Hydroxyproline / Q000032:analysis*; D055550:Protein Stability; D017433:Protein Structure, Secondary; D058767:Protein Unfolding; D037401:Scyphozoa / Q000737:chemistry*; D012995:Solubility; D055672:Static Electricity; D013816:Thermodynamics
chemical_list: D000596:Amino Acids; D003094:Collagen; D006909:Hydroxyproline
keywords: jellyfish collagen; pH dependency; secondary structure; thermal stability