title: Comparative proteomics analysis of the antitumor effect of CIGB-552 peptide in HT-29 colon adenocarcinoma cells.
abstract: The second generation peptide CIGB-552 has a pro-apoptotic effect on H460 non-small cell lung cancer cells and displays a potent cytotoxic effect in HT-29 colon adenocarcinoma cells though its action mechanism is ill defined. Here, we present the first proteomic study of peptide effect in HT-29 cells using subcellular fractionation, protein and peptide fractionation by DF-PAGE and LC-MS/MS peptide identification. In particular, we explored the nuclear proteome of HT-29 cells at a 5h treatment identifying a total of 68 differentially modulated proteins, 49 of which localize to the nucleus. The differentially modulated proteins were analyzed following a system biology approach. Results pointed to a modulation of apoptosis, oxidative damage removal, NF-κB activation, inflammatory signaling and of cell adhesion and motility. Further Western blot and flow-cytometry experiments confirmed both pro-apoptotic and anti-inflammatory effects of CIGB-552 peptide in HT-29 cells.
authors.affiliation: Department of Systems Biology, Center for Genetic Engineering and Biotechnology, Cuba.
authors.forename: Teresa
authors.initials: T
authors.lastname: Núñez de Villavicencio-Díaz
authors.affiliation: Department of Systems Biology, Center for Genetic Engineering and Biotechnology, Cuba.
authors.forename: Yassel
authors.initials: Y
authors.lastname: Ramos Gómez
authors.affiliation: Pharmaceuticals Department, Center for Genetic Engineering and Biotechnology, Cuba.
authors.forename: Brizaida
authors.initials: B
authors.lastname: Oliva Argüelles
authors.affiliation: Department of Systems Biology, Center for Genetic Engineering and Biotechnology, Cuba.
authors.forename: Julio R
authors.initials: JR
authors.lastname: Fernández Masso
authors.affiliation: Department of Systems Biology, Center for Genetic Engineering and Biotechnology, Cuba.
authors.forename: Arielis
authors.initials: A
authors.lastname: Rodríguez-Ulloa
authors.affiliation: Department of Preclinical Studies, National Institute of Oncology and Radiobiology of Cuba, Cuba.
authors.forename: Yiliam
authors.initials: Y
authors.lastname: Cruz García
authors.affiliation: Department of Systems Biology, Center for Genetic Engineering and Biotechnology, Cuba.
authors.forename: Osmany
authors.initials: O
authors.lastname: Guirola-Cruz
authors.affiliation: European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.
authors.forename: Yasset
authors.initials: Y
authors.lastname: Perez-Riverol
authors.affiliation: Department of Systems Biology, Center for Genetic Engineering and Biotechnology, Cuba.
authors.forename: Luis
authors.initials: L
authors.lastname: Javier González
authors.affiliation: Cell Biology Unit, Institut Pasteur of Montevideo, Uruguay.
authors.forename: Inés
authors.initials: I
authors.lastname: Tiscornia
authors.affiliation: Cell Biology Unit, Institut Pasteur of Montevideo, Uruguay.
authors.forename: Sabina
authors.initials: S
authors.lastname: Victoria
authors.affiliation: Cell Biology Unit, Institut Pasteur of Montevideo, Uruguay.
authors.forename: Mariela
authors.initials: M
authors.lastname: Bollati-Fogolín
authors.affiliation: Department of Systems Biology, Center for Genetic Engineering and Biotechnology, Cuba. Electronic address: vladimir.besada@cigb.edu.cu.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Besada Pérez
authors.affiliation: Pharmaceuticals Department, Center for Genetic Engineering and Biotechnology, Cuba.
authors.forename: Maribel
authors.initials: M
authors.lastname: Guerra Vallespi
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D057846:Cell-Penetrating Peptides / Q000494:pharmacology*; D003110:Colonic Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D006801:Humans; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D040901:Proteomics; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; C000598873:CIGB-552 peptide; D057846:Cell-Penetrating Peptides; D009363:Neoplasm Proteins
keywords: Apoptosis; CIGB-552 synthetic peptide; Colorectal cancer; Enrichment analysis; Inflammation; Text mining