title: Fostering the action of versatile peroxidase as a highly efficient biocatalyst for the removal of endocrine disrupting compounds.
abstract: Response surface methodology (RSM) was used to optimize the removal of five endocrine disrupting compounds (EDCs) by the enzyme versatile peroxidase (VP): bisphenol A (BPA), triclosan (TCS), estrone (E1), 17β-estradiol (E2) and 17α-ethinylestradiol (EE2). The optimal variables of enzyme activity (90-100 U L(-1)), sodium malonate (29-43 mM) and MnSO4 (0.8-1 mM) led to very high removal rates of the five pollutants (2.5-5.0 mg L(-1) min(-1)). The structural elucidation of transformation products arising from the enzymatic catalysis of the EDCs was investigated by Gas Chromatography coupled to Mass Spectrometry (GC-MS) and Liquid Chromatography Electrospray Time-of-Flight Mass Spectrometry (LC-ESI-TOF-MS). The presence of dimers and trimers, indicative of oxidative coupling, was demonstrated.
authors.affiliation: Department of Chemical Engineering, Institute of Technology, University of Santiago de Compostela, E-15782 Santiago de Compostela, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Taboada-Puig
authors.affiliation: Department of Chemical Engineering, Institute of Technology, University of Santiago de Compostela, E-15782 Santiago de Compostela, Spain. Electronic address: gemma.eibes@usc.es.
authors.forename: G
authors.initials: G
authors.lastname: Eibes
authors.affiliation: Department of Chemical Engineering, Institute of Technology, University of Santiago de Compostela, E-15782 Santiago de Compostela, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Lloret
authors.affiliation: Department of Chemical Engineering, Institute of Technology, University of Santiago de Compostela, E-15782 Santiago de Compostela, Spain.
authors.forename: T A
authors.initials: TA
authors.lastname: Lú-Chau
authors.affiliation: Department of Chemical Engineering, Institute of Technology, University of Santiago de Compostela, E-15782 Santiago de Compostela, Spain.
authors.forename: G
authors.initials: G
authors.lastname: Feijoo
authors.affiliation: Department of Chemical Engineering, Institute of Technology, University of Santiago de Compostela, E-15782 Santiago de Compostela, Spain.
authors.forename: M T
authors.initials: MT
authors.lastname: Moreira
authors.affiliation: Department of Chemical Engineering, Institute of Technology, University of Santiago de Compostela, E-15782 Santiago de Compostela, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Lema
mesh_terms: D055162:Biocatalysis; D001673:Biodegradation, Environmental; D052244:Endocrine Disruptors / Q000737:chemistry / Q000302:isolation & purification*; D008401:Gas Chromatography-Mass Spectrometry; D013058:Mass Spectrometry; D008970:Molecular Weight; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000378:metabolism*; D012044:Regression Analysis; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D052244:Endocrine Disruptors; D014874:Water Pollutants, Chemical; D009195:Peroxidase