title: Protecting the aged heart during cardiac surgery: single-dose del Nido cardioplegia is superior to multi-dose del Nido cardioplegia in isolated rat hearts.
abstract: BACKGROUND
  Aged hearts are particularly vulnerable to reperfusion injury. We recently showed that single-dose del Nido cardioplegia was superior to 'standard' multi-dose 4:1 blood cardioplegia in aged rat hearts. This study seeks to determine if multi-dose del Nido cardioplegia offers additional benefits over single-dose del Nido cardioplegia.
  
  
  METHODS
  Functional recovery after 60 min of cardioplegic arrest was assessed in isolated, working, senescent rat hearts. Single-dose del Nido cardioplegia (n=14) was compared to multi-dose del Nido cardioplegia (n=12) delivered every 20 min.
  
  
  RESULTS
  Troponin release during reperfusion was similar in the single (0.263 ± 0.056 ng/ml) and multi-dose groups (0.261 ± 0.055 ng/ml). Although functional recovery was similar early after reperfusion (stroke work 91 ± 6 ml*mmHg*g(-1) vs. 91 ± 8 ml*mmHg*g(-1) for single- vs. multi-dose), it declined over time in the multi-dose group (71 ± 9 vs. 43 ± 9 ml*mmHg*g(-1) at 60 min, p=0.0175) CONCLUSIONS: In aged rat hearts, a single-dose del Nido cardioplegia strategy results in superior functional recovery compared to a multi-dose del Nido cardioplegia strategy.
authors.affiliation: Department of Physiology and Biophysics, Dalhousie University, Halifax, NS, Canada.
authors.forename: Arun
authors.initials: A
authors.lastname: Govindapillai
authors.affiliation: Department of Surgery, Dalhousie University, Halifax, NS, Canada IWK Health Centre, Halifax, Nova Scotia, Canada.
authors.forename: Camille
authors.initials: C
authors.lastname: Hancock Friesen
authors.affiliation: Department of Physiology and Biophysics, Dalhousie University, Halifax, NS, Canada Department of Surgery, Dalhousie University, Halifax, NS, Canada IWK Health Centre, Halifax, Nova Scotia, Canada stacy.oblenes@iwk.nshealth.ca.
authors.forename: Stacy B
authors.initials: SB
authors.lastname: O'Blenes
mesh_terms: D000375:Aging; D000818:Animals; D002314:Cardioplegic Solutions / Q000494:pharmacology*; D006321:Heart / Q000503:physiopathology*; D006324:Heart Arrest, Induced / Q000379:methods*; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D002314:Cardioplegic Solutions
keywords: aged heart; del Nido cardioplegia; ischemia-reperfusion injury; working heart