title: Molecular and transcriptional characterization of GTHs and mPRα during ovarian maturation in rock bream Oplegnathus fasciatus.
abstract: The membrane progestin receptor α (mPRα) plays an important role in the regulation of oocyte meiotic maturation in fish. However, the endocrine regulation of multiple spawning in fish with asynchronous ovarian development remains to be determined. We identified four genes (FSHβ, LHβ, CGα and mPRα) that are widely expressed in both the reproductive and non-reproductive tissues of rock bream Oplegnathus fasciatus and examined their expression during ovarian maturation. The level of FSHβ transcripts in the pituitary remained high during vitellogenic stages, when the plasma levels of estradiol-17β reached maximum levels and oocyte development was stimulated. LHβ expression in the pituitary and ovary reached maximum levels during oocyte maturation when the plasma levels of testosterone also reached a maximum. A significant correlation between mPRα transcript levels and the gonadosomatic index was observed during vitellogenesis and final maturation. Brain gonadotropin (FSHβ and LHβ) and mPRα were continuously expressed at different stages of ovarian development. The dynamic and diverse nature of gonadotropins and mPRα gene expression patterns suggests that these genes might jointly regulate ovarian development and maturation through the brain-pituitary-ovary axis in the rock bream endocrine system.
authors.affiliation: Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Key Laboratory for the Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong, P.R. China.
authors.forename: Bao
authors.initials: B
authors.lastname: Shi
authors.affiliation: Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Key Laboratory for the Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong, P.R. China.
authors.forename: Xuezhou
authors.initials: X
authors.lastname: Liu
authors.affiliation: Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Key Laboratory for the Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong, P.R. China.
authors.forename: Yongjiang
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Key Laboratory for the Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong, P.R. China.
authors.forename: Zhongzhi
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Key Laboratory for the Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong, P.R. China.|College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, P.R. China.
authors.forename: Shengyi
authors.initials: S
authors.lastname: Chen
authors.affiliation: Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Key Laboratory for the Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong, P.R. China.|College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, P.R. China.
authors.forename: Kun
authors.initials: K
authors.lastname: Zang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D004958:Estradiol / Q000097:blood; D005260:Female; D037201:Follicle Stimulating Hormone, beta Subunit / Q000235:genetics*; D015870:Gene Expression; D005786:Gene Expression Regulation; D037101:Luteinizing Hormone, beta Subunit / Q000235:genetics*; D008969:Molecular Sequence Data; D009866:Oogenesis / Q000502:physiology; D010053:Ovary / Q000254:growth & development*; D010473:Perciformes / Q000235:genetics / Q000502:physiology*; D010902:Pituitary Gland / Q000378:metabolism; D011980:Receptors, Progesterone / Q000235:genetics*; D014818:Vitellogenesis / Q000502:physiology
chemical_list: D037201:Follicle Stimulating Hormone, beta Subunit; D037101:Luteinizing Hormone, beta Subunit; D011980:Receptors, Progesterone; D004958:Estradiol