title: Combination cell therapy with mesenchymal stem cells and neural stem cells for brain stroke in rats.
abstract: OBJECTIVE
  Brain stroke is the second most important events that lead to disability and morbidity these days. Although, stroke is important, there is no treatment for curing this problem. Nowadays, cell therapy has opened a new window for treating central nervous system disease. In some previous studies the Mesenchymal stem cells and neural stem cells. In this study, we have designed an experiment to assess the combination cell therapy (Mesenchymal and Neural stem cells) effects on brain stroke.
  
  
  METHODS
  The Mesenchymal stem cells were isolated from adult rat bone marrow and the neural stem cells were isolated from ganglion eminence of rat embryo 14 days. The Mesenchymal stem cells were injected 1 day after middle cerebral artery occlusion (MCAO) and the neural stem cells transplanted 7 day after MCAO. After 28 days, the neurological outcomes and brain lesion volumes were evaluated. Also, the activity of Caspase 3 was assessed in different groups.
  
  
  RESULTS
  The group which received combination cell therapy had better neurological examination and less brain lesion. Also the combination cell therapy group had the least Caspase 3 activity among the groups.
  
  
  CONCLUSIONS
  The combination cell therapy is more effective than Mesenchymal stem cell therapy and neural stem cell therapy separately in treating the brain stroke in rats.
authors.affiliation: Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran ; Cell and Molecular Student Research Group, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran ; Stem Cell laboratory, Department of Anatomy, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Seyed Mojtaba
authors.initials: SM
authors.lastname: Hosseini
authors.affiliation: Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran ; Cell and Molecular Student Research Group, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Farahmandnia
authors.affiliation: Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran ; Cell and Molecular Student Research Group, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Razi
authors.affiliation: Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran ; Cell and Molecular Student Research Group, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Somayeh
authors.initials: S
authors.lastname: Delavari
authors.affiliation: Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran ; Cell and Molecular Student Research Group, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Benafsheh
authors.initials: B
authors.lastname: Shakibajahromi
authors.affiliation: Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran ; Cell and Molecular Student Research Group, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Fatemeh Sabet
authors.initials: FS
authors.lastname: Sarvestani
authors.affiliation: Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran ; Cell and Molecular Student Research Group, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Sepehr
authors.initials: S
authors.lastname: Kazemi
authors.affiliation: Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran ; Cell and Molecular Student Research Group, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Semsar
keywords: Brain stroke; Combination cell therapy; Mesenchymal stem cells; Neural stem cells