title: Conditional control of dendritic cell factor 1 expression by a tetracycline-inducible system.
abstract: Dendritic cell factor 1 plays important roles in neural stem cells differentiation and in glioma cells proliferation, migration, and invasion. Here, we used a tetracycline&mdash;inducible system that regulates the expression of Dendritic cell factor 1 in glioma cells. We constructed two tet&mdash;inducible vectors, pTRE&mdash;EGFP&mdash;DCF1 and pTRE&mdash;LJM1&mdash;DCF1, by modifying the promoter PCMV. In the absence of tetracycline or doxycycline, the expression of Dendritic cell factor 1 in cells co&mdash;transfected with pTRE&mdash;EGFP&mdash;DCF1 or pTRE&mdash;LJM1&mdash;EGFP&mdash;DCF1 and ptTS&mdash;Neo was suppressed through binding of the tetracyline&mdash;controlled transcriptional suppressor to tetracycline response element, and the suppression was released by the addition of doxycycline. Our work has laid foundations for potential clinical application of cancer therapy in realizing artificial regulation of gene.
authors.affiliation: School of life sciences, Shanghai University Laboratory of molecular neural biology Shanghai China.
authors.forename: H
authors.initials: H
authors.lastname: Yan
authors.affiliation: School of life sciences, Shanghai University Laboratory of molecular neural biology Shanghai China.
authors.forename: C
authors.initials: C
authors.lastname: Huang
authors.affiliation: School of life sciences, Shanghai University Laboratory of molecular neural biology Shanghai China.
authors.forename: M
authors.initials: M
authors.lastname: Yang
authors.affiliation: School of life sciences, Shanghai University Laboratory of molecular neural biology Shanghai China.
authors.forename: J
authors.initials: J
authors.lastname: Guo
authors.affiliation: School of life sciences, Shanghai University Laboratory of molecular neural biology Shanghai China.
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of life sciences, Shanghai University Laboratory of molecular neural biology Shanghai China.
authors.forename: R
authors.initials: R
authors.lastname: Feng
authors.affiliation: School of life sciences, Shanghai University Laboratory of molecular neural biology Shanghai China wtq&commat;shu.edu.cn.
authors.forename: T
authors.initials: T
authors.lastname: Wen
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D017209:Apoptosis / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D003713:Dendritic Cells / Q000378:metabolism*; D004318:Doxycycline / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D005910:Glioma / Q000473:pathology*; D057809:HEK293 Cells; D006801:Humans; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D009361:Neoplasm Invasiveness / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D058953:Neural Stem Cells / Q000166:cytology*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding / Q000235:genetics; D020218:Response Elements / Q000235:genetics; D013752:Tetracycline / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C000606148:TMEM59 protein, human; D013752:Tetracycline; D004318:Doxycycline