title: Nanostructured lipid carriers based temozolomide and gene co-encapsulated nanomedicine for gliomatosis cerebri combination therapy.
abstract: BACKGROUND
  Co-delivery of gene and anticancer drug into the same cancer cells or tissues by multifunctional nanocarriers may provide a new paradigm in cancer treatment. In this study, nanostructured lipid carriers (NLCs) were constructed as multifunctional nanomedicine for co-delivery of enhanced green fluorescence protein plasmid (DNA) and temozolomide (TMZ).
  
  
  METHODS
  TMZ- and DNA-loaded NLCs (TMZ/DNA-NLCs) were prepared. Their particle size, zeta potential, gene-loading capacity (GL) and drug encapsulation efficiency (EE) were evaluated. In vitro cytotoxicity study TMZ/DNA-NLCs was tested in U87 malignant glioma cells (U87 MG cells). In vivo gene transfection and anti-tumor efficacy of the carriers were evaluated on mice bearing malignant glioma model.
  
  
  RESULTS
  The optimum TMZ/DNA-NLCs formulations with the particle size of 179 nm and with a +23 mV surface charge; got 91% of GL and 83% of EE. The growth of U87 MG cells in vitro was obviously inhibited. TMZ/DNA-NLCs also displayed the highest gene transfection efficiency and the best antitumor activity than other formulations in vivo.
  
  
  CONCLUSIONS
  The results demonstrated that TMZ/DNA-NLCs were efficient in selective delivery to malignant glioma cells. Also TMZ/DNA-NLCs transfer both drug and gene to the gliomatosis cerebri, enhance the antitumor capacity and gene transfection efficacy. Thus, TMZ/DNA-NLCs could prove to be a superior co-delivery nanomedicine to achieve therapeutic efficacy and this report could be a new promising strategy for treatment in malignant gliomatosis cerebri.
authors.affiliation: a Department of Neurosurgery , Second Affiliated Hospital, Nanchang University , Nanchang , Jiangxi , China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Chen
authors.affiliation: a Department of Neurosurgery , Second Affiliated Hospital, Nanchang University , Nanchang , Jiangxi , China.
authors.forename: Xianliang
authors.initials: X
authors.lastname: Lai
authors.affiliation: a Department of Neurosurgery , Second Affiliated Hospital, Nanchang University , Nanchang , Jiangxi , China.
authors.forename: Shuxin
authors.initials: S
authors.lastname: Song
authors.affiliation: a Department of Neurosurgery , Second Affiliated Hospital, Nanchang University , Nanchang , Jiangxi , China.
authors.forename: Xingen
authors.initials: X
authors.lastname: Zhu
authors.affiliation: a Department of Neurosurgery , Second Affiliated Hospital, Nanchang University , Nanchang , Jiangxi , China.
authors.forename: Jianming
authors.initials: J
authors.lastname: Zhu
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D045744:Cell Line, Tumor; D002626:Chemistry, Pharmaceutical; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000494:pharmacology*; D003606:Dacarbazine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D004337:Drug Carriers; D016503:Drug Delivery Systems; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D051379:Mice; D050997:Nanomedicine; D049329:Nanostructures; D018302:Neoplasms, Neuroepithelial / Q000737:chemistry / Q000188:drug therapy*; D000077204:Temozolomide; D014162:Transfection
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D008055:Lipids; D003606:Dacarbazine; D004247:DNA; D000077204:Temozolomide
keywords: Combination therapy; gene therapy; gliomatosis cerebri; nanostructured lipid carriers