title: Redox homeostasis and reactive oxygen species scavengers shift during ontogenetic phase changes in apple.
abstract: The change from juvenile to adult phase is a universal phenomenon in perennial plants such as apple. To validate the changes in hydrogen peroxide (H2O2) levels and scavenging during ontogenesis in apple seedlings, the H2O2 contents, its scavenging capacity, and the expression of related genes, as well as miR156 levels, were measured in leaf samples from different nodes in seedlings of 'Zisai Pearl' (Malus asiatica)×'Red Fuji' (M. domestica). Then in vitro shoots were treated with redox modulating chemicals to verify the response of miR156 to redox alteration. The expression of miR156 decreased gradually during ontogenesis, indicating a progressive loss of juvenility. During the phase changes, H2O2 and ascorbate contents, the ratio of ascorbate to dehydroascorbate, the ascorbate peroxidase, catalase and glutathione reductase activities, and the expressions of some MdGR and MdAPX gene family members increased remarkably. However, the glutathione content and glutathione to glutathione disulfide ratio declined. In chemicals treated in vitro shoots, the changes in miR156 levels were coordinated with GSH contents and GSH/GSSG ratio but not H2O2 contents. Conclusively, the relative reductive thiol redox status is critical for the maintenance of juvenility and the reductive ascorbate redox environment was elevated and sustained during the reproductive phase.
authors.affiliation: Institute for Horticultural Plants, China Agricultural University, No. 2 Yuanmingyuan West Rd, Beijing 100193, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Du
authors.affiliation: Institute for Horticultural Plants, China Agricultural University, No. 2 Yuanmingyuan West Rd, Beijing 100193, China.
authors.forename: Xiao Lin
authors.initials: XL
authors.lastname: Jia
authors.affiliation: Institute for Horticultural Plants, China Agricultural University, No. 2 Yuanmingyuan West Rd, Beijing 100193, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute for Horticultural Plants, China Agricultural University, No. 2 Yuanmingyuan West Rd, Beijing 100193, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wu
authors.affiliation: Institute for Horticultural Plants, China Agricultural University, No. 2 Yuanmingyuan West Rd, Beijing 100193, China.
authors.forename: Zhen Hai
authors.initials: ZH
authors.lastname: Han
authors.affiliation: Institute for Horticultural Plants, China Agricultural University, No. 2 Yuanmingyuan West Rd, Beijing 100193, China. Electronic address: zhangxinzhong999@126.com.
authors.forename: Xin Zhong
authors.initials: XZ
authors.lastname: Zhang
mesh_terms: D018506:Gene Expression Regulation, Plant; D006706:Homeostasis; D006861:Hydrogen Peroxide / Q000378:metabolism*; D027845:Malus / Q000235:genetics / Q000502:physiology*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D010084:Oxidation-Reduction; D018515:Plant Leaves / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D036226:Seedlings / Q000378:metabolism
chemical_list: D035683:MicroRNAs; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide
keywords: M. domestica; Malus asiatica; Phase change; Reduction and oxidation