title: Examination of metabolic responses to phosphorus limitation via proteomic analyses in the marine diatom Phaeodactylum tricornutum.
abstract: Phosphorus (P) is an essential macronutrient for the survival of marine phytoplankton. In the present study, phytoplankton response to phosphorus limitation was studied by proteomic profiling in diatom Phaeodactylum tricornutum in both cellular and molecular levels. A total of 42 non-redundant proteins were identified, among which 8 proteins were found to be upregulated and 34 proteins were downregulated. The results also showed that the proteins associated with inorganic phosphate uptake were downregulated, whereas the proteins involved in organic phosphorus uptake such as alkaline phosphatase were upregulated. The proteins involved in metabolic responses such as protein degradation, lipid accumulation and photorespiration were upregulated whereas energy metabolism, photosynthesis, amino acid and nucleic acid metabolism tend to be downregulated. Overall our results showed the changes in protein levels of P. tricornutum during phosphorus stress. This study preludes for understanding the role of phosphorous in marine biogeochemical cycles and phytoplankton response to phosphorous scarcity in ocean. It also provides insight into the succession of phytoplankton community, providing scientific basis for elucidating the mechanism of algal blooms.
authors.affiliation: Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, College of Life Science, Jinan University, Guangzhou 510632, China.
authors.forename: Tian-Ya
authors.initials: TY
authors.lastname: Feng
authors.affiliation: Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, College of Life Science, Jinan University, Guangzhou 510632, China.
authors.forename: Zhi-Kai
authors.initials: ZK
authors.lastname: Yang
authors.affiliation: Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, College of Life Science, Jinan University, Guangzhou 510632, China.
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, College of Life Science, Jinan University, Guangzhou 510632, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, College of Life Science, Jinan University, Guangzhou 510632, China.
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Li
authors.affiliation: Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, College of Life Science, Jinan University, Guangzhou 510632, China.
authors.forename: Shanmugaraj Bala
authors.initials: SB
authors.lastname: Murugan
authors.affiliation: Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, College of Life Science, Jinan University, Guangzhou 510632, China.
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Yang
authors.affiliation: Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, College of Life Science, Jinan University, Guangzhou 510632, China.
authors.forename: Jie-Sheng
authors.initials: JS
authors.lastname: Liu
authors.affiliation: Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, College of Life Science, Jinan University, Guangzhou 510632, China.
authors.forename: Hong-Ye
authors.initials: HY
authors.lastname: Li
mesh_terms: D059001:Aquatic Organisms / Q000235:genetics* / Q000378:metabolism; D017377:Diatoms / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D009584:Nitrogen; D010758:Phosphorus / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000235:genetics; D040901:Proteomics; D013312:Stress, Physiological / Q000235:genetics
chemical_list: D010758:Phosphorus; D009584:Nitrogen