title: High-speed droplet-allele-specific polymerase chain reaction for genotyping of single nucleotide polymorphisms.
abstract: Single nucleotide alternations such as single nucleotide polymorphisms (SNPs) or single nucleotide mutations are useful genetic markers for molecular diagnosis, prognosis, drug response, and predisposition to diseases. Rapid identification of SNPs or mutations is clinically important, especially for determining drug responses and selection of molecular-targeted therapy. Here, we describe a rapid genotyping assay based on the allele-specific polymerase chain reaction (AS-PCR) by using our droplet-PCR machine (droplet-AS-PCR).
authors.affiliation: Department of Laboratory Medicine, Shinshu University Hospital, 3-1-1 Asahi, Matsumoto, 390-8621, Japan, kmatsuda@shinshu-u.ac.jp.
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Matsuda
authors.forename: Takayuki
authors.initials: T
authors.lastname: Honda
mesh_terms: D000483:Alleles; D004247:DNA / Q000235:genetics*; D004867:Equipment Design; D060005:Genotyping Techniques / Q000191:economics / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D016133:Polymerase Chain Reaction / Q000191:economics / Q000295:instrumentation* / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D013997:Time Factors
chemical_list: D004247:DNA