title: [Identification of 3 novel HLA-A alleles A*24:224, A*24:225 and A*24:257 by sequence-based typing].
abstract: OBJECTIVE
  To verify 3 novel HLA-A alleles A*24:224, A*24:225 and A*24:257 identified in Chinese Han individuals.
  
  
  METHODS
  No full matched results were obtained at HLA-A locus in HLA typing for China Marrow Donor Program (CMDP) using bi-allelic Sequence-Based Typing (SBT). The novel HLA alleles were identified with allele-specific amplification SBT.
  
  
  RESULTS
  All of the three probands had a novel nucleotide sequence at HLA-A locus. All of the 3 new sequences are most close to HLA-A*24:02:01:01 except for 1 or 2 nucleotide substitution in exon 2, which resulted in different changes in corresponding codons and encoded amino acids.
  
  
  CONCLUSIONS
  Three novel HLA-A alleles were confirmed and officially named as HLA-A*24:224, HLA-A*24:225 and HLA-A*24:257 under the GenBank accession numbers JQ899198, JQ924283 and HG003642 by the WHO Nomenclature Committee for Factors of the HLA System in November 2012 and November 2013, respectively.
authors.affiliation: Blood Center of Shandong Province, Jinan, Shandong 250014, P.R.China. chuanfuzhu@aliyun.com.
authors.forename: Chuanfu
authors.initials: C
authors.lastname: Zhu
authors.forename: Hongwei
authors.initials: H
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiangmin
authors.initials: X
authors.lastname: Nie
mesh_terms: D000483:Alleles; D044466:Asian People / Q000208:ethnology / Q000235:genetics*; D001483:Base Sequence; D002681:China / Q000208:ethnology; D005828:Genetics, Population; D059871:HLA-A24 Antigen / Q000235:genetics*; D006650:Histocompatibility Testing; D006801:Humans; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
chemical_list: D059871:HLA-A24 Antigen