title: Regulation of the Heme Oxygenase-1/carbon monoxide system by hydrogen sulfide in murine coxsackievirus B3-induced myocarditis.
abstract: To explore the impact of hydrogen sulfide (H2S) on the heme oxygenase&mdash;1 (HO&mdash;1)/carbon monoxide (CO) system in coxsackie virus B3 (CVB3)&mdash;induced myocarditis. A total of 80 Balb/c mice were divided randomly into four groups designated N, C, P and S. Group N served as the negative control while groups C, P, and S were infected with CVB3 to induce myocarditis. Group P was additionally treated with DL&mdash;propargylglycine (PAG) to inhibit the generation of H2S while Group S was treated with NaHS, an H2S donor. Ten days after infection, heart sections were scored for histopathology. We also measured carboxyhemoglobin (COHb) levels in the blood and HO&mdash;1 expression by immunohistochemistry. 1. Each CVB3&mdash;infected group (C, P, and S) exhibited increased pathology, COHb levels, and HO&mdash;1 expression compared to uninfected controls. 2. Regarding histopathology, the score of group P was worse, while that of group S was better, than that of group C. 3. The P group COHb level was lower than group C, while the S group COHb level was higher than group C. 4. Positive HO&mdash;1 expression was seen in group C with reduced expression in group P and increased expression in group S. 5. A positive correlation was observed between the COHb concentration and HO&mdash;1expression; alternatively, a negative correlation was found between the histopathologic scores and both the concentration of COHb and the expression level of HO&mdash;1. Modulation of H2S can play a regulatory role in the pathogenesis of VMC by impacting the HO&mdash;1/CO pathway.
authors.affiliation: The Second Affiliated Hospital & Yuying Children&apos;s Hospital of Wenzhou Medical University Department of Pediatric Cardiology Wenzhou China.
authors.forename: S
authors.initials: S
authors.lastname: Zhang
authors.affiliation: The Second Affiliated Hospital & Yuying Children&apos;s Hospital of Wenzhou Medical University Department of Pediatric Cardiology Wenzhou China.
authors.forename: T
authors.initials: T
authors.lastname: Wu
authors.affiliation: The Second Affiliated Hospital & Yuying Children&apos;s Hospital of Wenzhou Medical University Department of Pediatric Cardiology Wenzhou China.
authors.forename: T
authors.initials: T
authors.lastname: Xia
authors.affiliation: The Second Affiliated Hospital & Yuying Children&apos;s Hospital of Wenzhou Medical University Department of Pediatric Cardiology Wenzhou China.
authors.forename: X
authors.initials: X
authors.lastname: Rong
authors.affiliation: The Second Affiliated Hospital & Yuying Children&apos;s Hospital of Wenzhou Medical University Department of Pediatric Cardiology Wenzhou China.
authors.forename: T
authors.initials: T
authors.lastname: Wu
authors.affiliation: The Second Affiliated Hospital & Yuying Children&apos;s Hospital of Wenzhou Medical University Department of Pediatric Cardiology Wenzhou China.
authors.forename: M
authors.initials: M
authors.lastname: Chu
authors.affiliation: The Second Affiliated Hospital & Yuying Children&apos;s Hospital of Wenzhou Medical University Department of Pediatric Cardiology Wenzhou China rongzhouwu&commat;outlook.com.
authors.forename: R
authors.initials: R
authors.lastname: Wu
mesh_terms: D000480:Alkynes / Q000494:pharmacology; D000818:Animals; D002248:Carbon Monoxide / Q000378:metabolism*; D002263:Carboxyhemoglobin / Q000378:metabolism*; D029822:Enterovirus B, Human; D005998:Glycine / Q000031:analogs & derivatives / Q000494:pharmacology; D006367:HeLa Cells; D051547:Heme Oxygenase-1 / Q000378:metabolism*; D006801:Humans; D006862:Hydrogen Sulfide / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009205:Myocarditis / Q000821:virology*; D032383:Myocytes, Cardiac / Q000378:metabolism; D011897:Random Allocation
chemical_list: D000480:Alkynes; C009055:propargylglycine; D002248:Carbon Monoxide; D002263:Carboxyhemoglobin; D051547:Heme Oxygenase-1; D005998:Glycine; D006862:Hydrogen Sulfide