title: Genotyping of Human Platelet Antigens by BeadChip Microarray Technology.
abstract: Human platelet antigen (HPA) typing plays a critical role in the diagnosis of fetal/neonatal alloimmune thrombocytopenia, and the prevention of posttransfusion purpura and refractoriness to platelet transfusions. The recent development of high-throughput genotyping methods, allowing simultaneous genotyping of as many as 17 HPAs, is of utmost interest for saving time and money. Here, we describe a microarray technology named "BeadChip," designed for HPA-1 to -9, -11, and -15 genotyping of up to 96 individuals, in approximately 5 h. This technology was used to study allele frequencies in Brazilian blood donors, considering the heterogeneous ethnic composition.
authors.affiliation: Histocompatibilty and Immunogenetics Laboratory, EFS Bretagne, Rue Pierre Jean-Gineste, 35000, Rennes, France, gerald.bertrand@efs.sante.fr.
authors.forename: Gerald
authors.initials: G
authors.lastname: Bertrand
authors.forename: Fabiana
authors.initials: F
authors.lastname: Conti
mesh_terms: D016824:Antigens, Human Platelet / Q000235:genetics*; D001938:Brazil; D004867:Equipment Design; D005787:Gene Frequency; D005838:Genotype; D060005:Genotyping Techniques / Q000295:instrumentation / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods; D011696:Purpura, Thrombocytopenic / Q000175:diagnosis / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000295:instrumentation / Q000379:methods; D013921:Thrombocytopenia / Q000175:diagnosis / Q000235:genetics; D065227:Transfusion Reaction
chemical_list: D016824:Antigens, Human Platelet