title: [The comparative characteristic of cytokine production in serum and whole blood in patients with chronic autoimmune nettle rash].
abstract: The sampling of 100 patients with chronic autoimmune nettle rash and control group of 30 healthy donors was analyzed for identification of level of interleukin (IL)-4, IL-10, IL-17, IL-18 and γ-interferon (IFN) in serum of patients. The same sampling was examined using method ex vivo for spontaneous and induced production by cells of immune system. In patients with chronic autoimmune nettle rash increasing of spontaneous production of IL-4 and spontaneous and induced production of IL-17 and IFN was identified. The decreasing of spontaneous and induced production of IL-18 was detected too. These occurrences indicate to simultaneous activation of Th1, Th2 and Th17-population of T-lymphocytes. The analysis of level of cytokines in blood serum established only decreasing of level of IL-4 in patients with chronic autoimmune nettle rash as compared with healthy individuals. The level of other analyzed cytokines had no reliable differences that demonstrate both low informativeness of detection ofcontent of cytokines in blood serum and advantage of application of method ex vivo with detection of level of cytokines in whole blood.
authors.forename: L A
authors.initials: LA
authors.lastname: Aschina
authors.forename: N I
authors.initials: NI
authors.lastname: Baranova
authors.forename: S V
authors.initials: SV
authors.lastname: Kojenkova
mesh_terms: D000293:Adolescent; D000328:Adult; D001327:Autoimmune Diseases / Q000097:blood* / Q000473:pathology; D005076:Exanthema / Q000097:blood* / Q000473:pathology; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000097:blood; D016753:Interleukin-10 / Q000097:blood; D020381:Interleukin-17 / Q000097:blood; D020382:Interleukin-18 / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology
chemical_list: C508609:IL10 protein, human; C508717:IL17A protein, human; C000589760:IL18 protein, human; C508598:IL4 protein, human; D020381:Interleukin-17; D020382:Interleukin-18; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma