title: Molecular mechanism of serine/threonine protein phosphatase 1 (PP1cα-PP1r7) in spermatogenesis of Toxocara canis.
abstract: Toxocariasis is one of the most important, but neglected, zoonoses, which is mainly caused by Toxocara canis. To better understand the role of serine/threonine protein phosphatase 1 (PP1) in reproductive processes of male adult T. canis, differential expression analysis was used to reveal the profiles of PP1 catalytic subunit α (PP1cα) gene Tc-stp-1 and PP1 regulatory subunit 7 (PP1r7) gene TcM-1309. Indirect fluorescence immunocytochemistry was carried out to determine the subcellular distribution of PP1cα. Double-stranded RNA interference (RNAi) assays were employed to illustrate the function and mechanism of PP1cα in male adult reproduction. Real-time quantitative PCR (qPCR) showed transcriptional consistency of Tc-stp-1 and TcM-1309 in sperm-producing germline tissues and localization research showed cytoplasmic distribution of PP1cα in sf9 cells, which indicated relevant involvements of PP1cα and PP1r7 in spermatogenesis. Moreover, spatiotemporal transcriptional differences of Tc-stp-1 were determined by gene knockdown analysis, which revealed abnormal morphologies and blocked meiotic divisions of spermatocytes by phenotypic aberration scanning, thereby highlighting the crucial involvement of PP1cα in spermatogenesis. These results revealed a PP1cα-PP1r7 mechanism by which PP1 regulates kinetochore-microtubule interactions in spermatogenesis and provided important clues to identify novel drug or vaccine targets for toxocariasis control.
authors.affiliation: Department of Veterinary Medicine, Rongchang Campus, Southwest University, Chongqing 402460, People's Republic of China.
authors.forename: Guang Xu
authors.initials: GX
authors.lastname: Ma
authors.affiliation: Department of Veterinary Medicine, Rongchang Campus, Southwest University, Chongqing 402460, People's Republic of China. Electronic address: rongqiongzhou@126.com.
authors.forename: Rong Qiong
authors.initials: RQ
authors.lastname: Zhou
authors.affiliation: Department of Veterinary Medicine, Rongchang Campus, Southwest University, Chongqing 402460, People's Republic of China.
authors.forename: Zhen Hui
authors.initials: ZH
authors.lastname: Song
authors.affiliation: Department of Veterinary Medicine, Rongchang Campus, Southwest University, Chongqing 402460, People's Republic of China.
authors.forename: Hong Hong
authors.initials: HH
authors.lastname: Zhu
authors.affiliation: Department of Veterinary Medicine, Rongchang Campus, Southwest University, Chongqing 402460, People's Republic of China.
authors.forename: Zuo Yong
authors.initials: ZY
authors.lastname: Zhou
authors.affiliation: College of Life Sciences, Southwest University, Chongqing 402460, People's Republic of China.
authors.forename: Yuan Qin
authors.initials: YQ
authors.lastname: Zeng
mesh_terms: D000818:Animals; D018386:Kinetochores / Q000378:metabolism*; D008297:Male; D008870:Microtubules / Q000378:metabolism*; D054645:Protein Phosphatase 1 / Q000378:metabolism*; D012694:Serine / Q000378:metabolism; D013091:Spermatogenesis / Q000502:physiology*; D013912:Threonine / Q000378:metabolism; D017166:Toxocara canis / Q000378:metabolism*
chemical_list: D013912:Threonine; D012694:Serine; D054645:Protein Phosphatase 1
keywords: Reproduction; Serine/threonine protein phosphatase 1; Spermatogenesis; Toxocariasis