title: Assessment of neutralizing and non-neutralizing antibody responses against Porcine circovirus 2 in vaccinated and non-vaccinated farmed pigs.
abstract: Vaccination is the most efficacious procedure to curtail Porcine circovirus 2 (PCV2)-associated diseases (PCVAD). Experimental studies indicate that PCV2 vaccine-induced virus-neutralizing antibodies play a major role in protection from PCVAD. However, the immune response to PCV2 vaccination of pigs on farms is less clear. Analysing groups of age-matched vaccinated and non-vaccinated farmed pigs, we found significantly increased levels of virus-neutralizing antibodies only in vaccinated pigs belonging to the age group with the highest risk for developing PCVAD. Serum levels of PCV2 genomes were not different between corresponding age groups. Levels of antibodies directed against a linear peptide from the PCV2 capsid protein correlated with those of virus-neutralizing antibodies and reached the highest levels in older, non-vaccinated animals, pointing towards an intense interaction between PCV2-infected cells and the immune system. In conclusion, current PCV2 vaccines are in need of improvement to induce stronger and more rapid immunity to prevent PCV2 infection.
authors.affiliation: Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta, Canada.
authors.forename: Cristina
authors.initials: C
authors.lastname: Solis Worsfold
authors.affiliation: Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta, Canada.
authors.forename: Rkia
authors.initials: R
authors.lastname: Dardari
authors.affiliation: Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta, Canada.
authors.forename: Sampson
authors.initials: S
authors.lastname: Law
authors.affiliation: Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta, Canada.
authors.forename: Michael
authors.initials: M
authors.lastname: Eschbaumer
authors.affiliation: Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta, Canada.
authors.forename: Narges
authors.initials: N
authors.lastname: Nourozieh
authors.affiliation: Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta, Canada.|Marshall Swine and Poultry Health Services, Camrose, Alberta, Canada.
authors.forename: Frank
authors.initials: F
authors.lastname: Marshall
authors.affiliation: Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta, Canada.
authors.forename: Markus
authors.initials: M
authors.lastname: Czub
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D018173:Circoviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D005260:Female; D008297:Male; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D014611:Vaccination; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D014765:Viral Vaccines