title: Genetic polymorphisms in Toll-like receptor 3 gene are associated with the risk of hepatitis B virus-related liver diseases in a Chinese population.
abstract: Toll-like receptors (TLRs) are well known to play a critical role in antiviral and antitumor response. Available evidence has demonstrated that TLRs are implicated in the immunopathogenesis of hepatitis B virus (HBV) infection and tumor carcinogenesis. Therefore, we performed the present study to investigate the relationship with TLR3 gene polymorphisms and the risk of HBV-related liver disease in a Chinese population. A total of 623 individuals were included: 172 chronic hepatitis B (CHB) patients, 91 HBV-related liver cirrhosis (LC) patients, 174 HBV-related hepatocellular carcinoma (HCC) patients, and 186 healthy controls. Allelic and genotypic frequencies of TLR3 rs1879026 and rs3775290 polymorphisms were detected by PCR-RFLP and DNA sequencing analysis. Association analysis showed that the TT genotype of TLR3 rs3775290 was associated with a decreased risk for CHB, HBV-related LC, and HCC (OR=0.52, 95% CI: 0.27-0.99, P=0.048; OR=0.32, 95% CI: 0.14-0.76, P=0.010; OR=0.49, 95% CI: 0.26-0.92, P=0.027). Nevertheless, a lack of association was found between TLR3 rs1879026 and HBV-related liver diseases. In addition, haplotype analysis revealed that individuals who carried the GT haplotype might have a decreased risk of HBV-related liver diseases. The results indicated that genetic variant in TLR3 gene rs3775290 polymorphisms may be a protective factor for CHB, HBV-related LC, and HCC in the Chinese population.
authors.affiliation: Department of Clinical Laboratory, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China. Electronic address: huang_xiamei@163.com.
authors.forename: Xiamei
authors.initials: X
authors.lastname: Huang
authors.affiliation: Department of Clinical Laboratory, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China.
authors.forename: Haiwei
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratory, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China.
authors.forename: Chunni
authors.initials: C
authors.lastname: Huang
authors.affiliation: Department of Clinical Laboratory, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Clinical Laboratory, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China. Electronic address: qinxue919@126.com.
authors.forename: Xue
authors.initials: X
authors.lastname: Qin
authors.affiliation: Department of Clinical Laboratory, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China. Electronic address: lis8858@126.com.
authors.forename: Shan
authors.initials: S
authors.lastname: Li
mesh_terms: D044466:Asian People / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000821:virology; D020022:Genetic Predisposition to Disease; D006515:Hepatitis B virus; D019694:Hepatitis B, Chronic / Q000150:complications* / Q000235:genetics* / Q000821:virology; D006801:Humans; D008103:Liver Cirrhosis / Q000235:genetics / Q000821:virology; D008113:Liver Neoplasms / Q000235:genetics / Q000821:virology; D020641:Polymorphism, Single Nucleotide; D051196:Toll-Like Receptor 3 / Q000235:genetics*
chemical_list: D051196:Toll-Like Receptor 3
keywords: Chronic hepatitis B; Hepatocellular carcinoma; Liver cirrhosis; Polymorphism; Toll-like receptor 3