title: PURE-LAMP Procedure for the Diagnosis of Extrapulmonary Tuberculosis: A Case Series.
abstract: Although the polymerase chain reaction is effective for the diagnosis of extrapulmonary tuberculosis (EPTB), it is typically unavailable in resource-limited situations. In contrast, the loop-mediated isothermal amplification (LAMP) assay is a relatively cost-effective and accessible method. Additionally, when combined with the procedure for ultra-rapid extraction (PURE) kit, which enables simple DNA extraction, LAMP can detect Mycobacterium tuberculosis in sputum within 1.5 hours using a simple procedure. In this study, we investigated the utility of the PURE-LAMP technique to diagnose three cases of EPTB and showed that it may potentially be a valuable tool for the diagnosis of EPTB.
authors.affiliation: Division of Infectious Diseases and Pulmonary Medicine, Department of Internal Medicine, National Defense Medical College, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Kouzaki
authors.forename: Kei
authors.initials: K
authors.lastname: Mikita
authors.forename: Takuya
authors.initials: T
authors.lastname: Maeda
authors.forename: Masashi
authors.initials: M
authors.lastname: Ishihara
authors.forename: Shuichi
authors.initials: S
authors.lastname: Kawano
authors.forename: Yuji
authors.initials: Y
authors.lastname: Fujikura
authors.forename: Kazuo
authors.initials: K
authors.lastname: Imai
authors.forename: Soichiro
authors.initials: S
authors.lastname: Kanoh
authors.forename: Kazuho
authors.initials: K
authors.lastname: Utsunomiya
authors.forename: Masahiro
authors.initials: M
authors.lastname: Inoue
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Miyahira
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kawana
mesh_terms: D000328:Adult; D000369:Aged, 80 and over; D003362:Cost-Benefit Analysis; D005260:Female; D006801:Humans; D008297:Male; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013183:Sputum / Q000382:microbiology*; D014376:Tuberculosis / Q000175:diagnosis*