title: Metabolic and structural integrity of magnetic nanoparticle-loaded primary endothelial cells for targeted cell therapy.
abstract: OBJECTIVE
  To successfully translate magnetically mediated cell targeting from bench to bedside, there is a need to systematically assess the potential adverse effects of magnetic nanoparticles (MNPs) interacting with 'therapeutic' cells. Here, we examined in detail the effects of internalized polymeric MNPs on primary rat endothelial cells' structural intactness, metabolic integrity and proliferation potential.
  
  
  METHODS
  The intactness of cytoskeleton and organelles was studied by fluorescent confocal microscopy, flow cytometry and high-resolution respirometry.
  
  
  RESULTS
  MNP-loaded primary endothelial cells preserve intact cytoskeleton and organelles, maintain normal rate of proliferation, calcium signaling and mitochondria energy metabolism.
  
  
  CONCLUSIONS
  This study provides supportive evidence that MNPs at doses necessary for targeting did not induce significant adverse effects on structural integrity and functionality of primary endothelial cells - potential cell therapy vectors.
authors.affiliation: 1Department of Surgery, Drexel University College of Medicine, Philadelphia, PA 19102, USA.
authors.forename: Zulfiya
authors.initials: Z
authors.lastname: Orynbayeva
authors.affiliation: 2Department of Surgery, University of Pennsylvania, Philadelphia, PA 19104, USA.
authors.forename: Richard
authors.initials: R
authors.lastname: Sensenig
authors.affiliation: 1Department of Surgery, Drexel University College of Medicine, Philadelphia, PA 19102, USA.
authors.forename: Boris
authors.initials: B
authors.lastname: Polyak
mesh_terms: D000818:Animals; D064987:Cell- and Tissue-Based Therapy; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology*; D008280:Magnetics; D053758:Nanoparticles; D051381:Rats
keywords: Ca2+ signaling; cytoskeleton; magnetic nanoparticles; mitochondria membrane potential; mitochondria oxidative phosphorylation; primary endothelial cells