title: Next generation sequencing yields complete mitogenomes of Leopard whipray (Himantura leoparda) and Blue-spotted stingray (Neotrygon kuhlii) (Chondrichthyes: Dasyatidae).
abstract: The Leopard whipray (Himantura leoparda) and Blue-spotted stingray (Neotrygon kuhlii) are distributed in the Indian and West Pacific Ocean and considered as complex species based on morphological and molecular evidences. In this study, we used the next-generation sequencing method to decode two complete mitogenomes of H. leoparda and N. kuhlii. The assembled mitogenome, consisting lengths of 17,690 bp for H. leoparda and 17,974 bp for N. kuhlii, shows 78% identity to each other. Both mitogenomes follow the typical vertebrate arrangement, including 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes and a non-coding control region of D-loop. D-loop with the lengths 1931 bp (H. leoparda) and 2243 bp (N. kuhlii) is located between tRNA-Pro and tRNA-Phe. The overall GC content is 40.3% for H. leoparda and 39.8% for N. kuhlii. The complete mitogenome of H. leoparda and N. kuhlii provides essential and important DNA molecular data for further phylogenetic and evolutionary analyses for stingray species complex.
authors.affiliation: a Center of Excellence for the Oceans, National Taiwan Ocean University , Keelung , Taiwan .
authors.forename: Kang-Ning
authors.initials: KN
authors.lastname: Shen
authors.affiliation: b Department of Biology , National Museum of Marine Biology and Aquarium , Checheng, Pingtung , Taiwan .|c Graduate Institute of Marine Biodiversity & Evolutionary Biology, National Dong Hwa University , Checheng, Pingtung , Taiwan .
authors.forename: Chih-Wei
authors.initials: CW
authors.lastname: Chang
authors.affiliation: d Department of Bioscience Technology , Chung Yuan Christian University , Chung-Li , Taiwan .
authors.forename: Shiou-Yi
authors.initials: SY
authors.lastname: Tsai
authors.affiliation: d Department of Bioscience Technology , Chung Yuan Christian University , Chung-Li , Taiwan .
authors.forename: Shan-Chun
authors.initials: SC
authors.lastname: Wu
authors.affiliation: d Department of Bioscience Technology , Chung Yuan Christian University , Chung-Li , Taiwan .
authors.forename: Zi-Han
authors.initials: ZH
authors.lastname: Lin
authors.affiliation: d Department of Bioscience Technology , Chung Yuan Christian University , Chung-Li , Taiwan .
authors.forename: Yen-Fan
authors.initials: YF
authors.lastname: Chan
authors.affiliation: e WeThink Biotech Inc. , Taoyuan , Taiwan , and.
authors.forename: Ching-Hung
authors.initials: CH
authors.lastname: Chen
authors.affiliation: d Department of Bioscience Technology , Chung Yuan Christian University , Chung-Li , Taiwan .
authors.forename: Chung-Der
authors.initials: CD
authors.lastname: Hsiao
authors.affiliation: f IRD-UMR 250, eMtroPI c/o Universitas Udayana , Denpasar , Indonesie.
authors.forename: Philippe
authors.initials: P
authors.lastname: Borsa
mesh_terms: D000818:Animals; D001482:Base Composition / Q000235:genetics; D054629:Genome, Mitochondrial / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D012860:Skates, Fish / Q000235:genetics
chemical_list: D012335:RNA, Ribosomal; D012343:RNA, Transfer
keywords: Blue-spotted stingray; leopard whipray; mitogenome; next generation sequencing