title: HIF-1α-induced microRNA-210 reduces hypoxia-induced osteoblast MG-63 cell apoptosis.
abstract: To better understand the ischemic-hypoxia-induced fracture healing impairment, we determined in this study the microRNA-210 expression in broken bone specimens and in osteoblasts under hypoxia and then determined the influence of microRNA-210 overexpression on the osteoblast cell proliferation and apoptosis. Results demonstrated that microRNA-210 expression was upregulated with an association with HIF-1α overexpression in clinical human catagmatic tissues and was upregulated HIF-1α-dependently in response to hypoxia in osteoblast MG-63 cells. CCK-8 assay indicated that microRNA-210 upregulation by microRNA-210 mimics reduced the chemotherapeutic 5-FU-induced osteoblast cell death, and colony formation assay demonstrated that microRNA-210 mimics promoted osteoblast cells growth. Moreover, the microRNA-210 mimics transfection inhibited the hypoxia-induced MG-63 cell apoptosis via inhibiting the activation of caspase 3 and caspase 9. Therefore, our research indicated a protective role of microRNA-210 in response to hypoxia. And microRNA-210 might serve as a protective role in bone fracture healing.
authors.affiliation: a Orthopaedics Department , Renmin Hospital of Wuhan University , Wuhan , China.
authors.forename: Guanwen
authors.initials: G
authors.lastname: Sun
authors.forename: Hao
authors.initials: H
authors.lastname: Peng
mesh_terms: D017209:Apoptosis / Q000235:genetics; D015687:Cell Hypoxia / Q000235:genetics; D002460:Cell Line; D049109:Cell Proliferation / Q000235:genetics*; D017102:Fracture Healing / Q000235:genetics*; D050723:Fractures, Bone / Q000235:genetics / Q000473:pathology; D005786:Gene Expression Regulation; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000096:biosynthesis* / Q000235:genetics; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D010006:Osteoblasts / Q000378:metabolism / Q000473:pathology
chemical_list: C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; C543800:MIRN210 microRNA, human; D035683:MicroRNAs
keywords: apoptosis; bone fracture; hypoxia; microRNA-210; osteoblasts