title: Comparative Genomics Within the Bacillus Genus Reveal the Singularities of Two Robust Bacillus amyloliquefaciens Biocontrol Strains.
abstract: Bacillus amyloliquefaciens CECT 8237 and CECT 8238, formerly known as Bacillus subtilis UMAF6639 and UMAF6614, respectively, contribute to plant health by facing microbial pathogens or inducing the plant's defense mechanisms. We sequenced their genomes and developed a set of ad hoc scripts that allowed us to search for the features implicated in their beneficial interaction with plants. We define a core set of genes that should ideally be found in any beneficial Bacillus strain, including the production of secondary metabolites, volatile compounds, metabolic plasticity, cell-to-cell communication systems, and biofilm formation. We experimentally prove that some of these genetic elements are active, such as i) the production of known secondary metabolites or ii) acetoin and 2-3-butanediol, compounds that stimulate plant growth and host defense responses. A comparison with other Bacillus genomes permits us to find differences in the cell-to-cell communication system and biofilm formation and to hypothesize variations in their persistence and resistance ability in diverse environmental conditions. In addition, the major protection provided by CECT 8237 and CECT 8238, which is different from other Bacillus strains against bacterial and fungal melon diseases, permits us to propose a correlation with their singular genetic background and determine the need to search for additional blind biocontrol-related features.
authors.affiliation: 1 Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora" (IHSM-UMA-CSIC), Departamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, Bulevar Louis Pasteur 31 (Campus Universitario de Teatinos), 29071 Málaga, Spain;
authors.forename: M C
authors.initials: MC
authors.lastname: Magno-Pérez-Bryan
authors.affiliation: 2 Centro de Biotecnología y Genómica de Plantas UPM-INIA (CBGP), Parque Científico y Tecnológico de la Universidad Politécnica de Madrid, Campus de Montegancedo, 28223 Pozuelo de Alarcón, Madrid, Spain;|3 Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora" (IHSM-UMA-CSIC), Área de Genética, Facultad de Ciencias, Universidad de Málaga.
authors.forename: P M
authors.initials: PM
authors.lastname: Martínez-García
authors.affiliation: 1 Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora" (IHSM-UMA-CSIC), Departamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, Bulevar Louis Pasteur 31 (Campus Universitario de Teatinos), 29071 Málaga, Spain;
authors.forename: J
authors.initials: J
authors.lastname: Hierrezuelo
authors.affiliation: 2 Centro de Biotecnología y Genómica de Plantas UPM-INIA (CBGP), Parque Científico y Tecnológico de la Universidad Politécnica de Madrid, Campus de Montegancedo, 28223 Pozuelo de Alarcón, Madrid, Spain;
authors.forename: P
authors.initials: P
authors.lastname: Rodríguez-Palenzuela
authors.affiliation: 1 Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora" (IHSM-UMA-CSIC), Departamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, Bulevar Louis Pasteur 31 (Campus Universitario de Teatinos), 29071 Málaga, Spain;
authors.forename: E
authors.initials: E
authors.lastname: Arrebola
authors.affiliation: 3 Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora" (IHSM-UMA-CSIC), Área de Genética, Facultad de Ciencias, Universidad de Málaga.
authors.forename: C
authors.initials: C
authors.lastname: Ramos
authors.affiliation: 1 Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora" (IHSM-UMA-CSIC), Departamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, Bulevar Louis Pasteur 31 (Campus Universitario de Teatinos), 29071 Málaga, Spain;
authors.forename: A
authors.initials: A
authors.lastname: de Vicente
authors.affiliation: 1 Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora" (IHSM-UMA-CSIC), Departamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, Bulevar Louis Pasteur 31 (Campus Universitario de Teatinos), 29071 Málaga, Spain;
authors.forename: A
authors.initials: A
authors.lastname: Pérez-García
authors.affiliation: 1 Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora" (IHSM-UMA-CSIC), Departamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, Bulevar Louis Pasteur 31 (Campus Universitario de Teatinos), 29071 Málaga, Spain;
authors.forename: D
authors.initials: D
authors.lastname: Romero
mesh_terms: D001407:Bacillus / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D019662:Cucurbitaceae / Q000382:microbiology*; D016680:Genome, Bacterial / Q000235:genetics*; D023281:Genomics; D008969:Molecular Sequence Data; D005810:Multigene Family; D010572:Pest Control, Biological; D010641:Phenotype; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology / Q000517:prevention & control*; D018515:Plant Leaves / Q000382:microbiology; D012639:Seeds / Q000382:microbiology; D017422:Sequence Analysis, DNA
chemical_list: D001426:Bacterial Proteins