title: New Anti-Inflammatory Cembranes from the Cultured Soft Coral Nephthea columnaris.
abstract: Two new cembranes, columnariols A (1) and B (2), were isolated from the cultured soft coral Nephthea columnaris. The structures of cembranes 1 and 2 were elucidated by spectroscopic methods. In the anti-inflammatory effects test, cembranes 1 and 2 were found to significantly inhibit the accumulation of the pro-inflammatory iNOS and COX-2 protein of the lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. Compound 1 exhibited moderate cytotoxicity toward LNCaP cells with an IC50 value of 9.80 μg/mL.
authors.affiliation: Graduate Institute of Marine Biology, National Dong Hwa University, Pingtung 944, Taiwan. hsiaoinon@gmail.com.|National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan. hsiaoinon@gmail.com.
authors.forename: Ting-Hsi
authors.initials: TH
authors.lastname: Hsiao
authors.affiliation: Department of Anesthesiology, Taipei Veterans General Hospital, Taipei 112, Taiwan. sung6119@gmail.com.|School of Medicine, National Yang-Ming University, Taipei 112, Taiwan. sung6119@gmail.com.
authors.forename: Chun-Sung
authors.initials: CS
authors.lastname: Sung
authors.affiliation: School of Pharmacy, College of Pharmacy, China Medical University, Taichung 404, Taiwan. lanyh@mail.cmu.edu.tw.
authors.forename: Yu-Hsuan
authors.initials: YH
authors.lastname: Lan
authors.affiliation: Department of Marine Biotechnology and Resources, Asia-Pacific Ocean Research Center, National Sun Yat-sen University, Kaohsiung 804, Taiwan. cvyc.wang@gmail.com.|Division of Cardiology, Department of Internal Medicine, Kaohsiung Armed Forces General Hospital, Kaohsiung 802, Taiwan. cvyc.wang@gmail.com.
authors.forename: Yi-Chen
authors.initials: YC
authors.lastname: Wang
authors.affiliation: Graduate Institute of Marine Biology, National Dong Hwa University, Pingtung 944, Taiwan. jinx6609@nmmba.gov.tw.|National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan. jinx6609@nmmba.gov.tw.
authors.forename: Mei-Chin
authors.initials: MC
authors.lastname: Lu
authors.affiliation: Department of Marine Biotechnology and Resources, Asia-Pacific Ocean Research Center, National Sun Yat-sen University, Kaohsiung 804, Taiwan. wzh@mail.nsysu.edu.tw.
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Wen
authors.affiliation: School of Pharmacy, College of Pharmacy, China Medical University, Taichung 404, Taiwan. yachwu@mail.cmu.edu.tw.|Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung 404, Taiwan. yachwu@mail.cmu.edu.tw.|Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan. yachwu@mail.cmu.edu.tw.|Center for Molecular Medicine, China Medical University Hospital, Taichung 404, Taiwan. yachwu@mail.cmu.edu.tw.
authors.forename: Yang-Chang
authors.initials: YC
authors.lastname: Wu
authors.affiliation: Graduate Institute of Marine Biology, National Dong Hwa University, Pingtung 944, Taiwan. pjsung@nmmba.gov.tw.|National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan. pjsung@nmmba.gov.tw.|Department of Marine Biotechnology and Resources, Asia-Pacific Ocean Research Center, National Sun Yat-sen University, Kaohsiung 804, Taiwan. pjsung@nmmba.gov.tw.|Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung 404, Taiwan. pjsung@nmmba.gov.tw.|Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan. pjsung@nmmba.gov.tw.
authors.forename: Ping-Jyun
authors.initials: PJ
authors.lastname: Sung
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000737:chemistry*; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D045744:Cell Line, Tumor; D051546:Cyclooxygenase 2 / Q000378:metabolism; D004224:Diterpenes / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D020128:Inhibitory Concentration 50; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D004224:Diterpenes; D008070:Lipopolysaccharides; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2
keywords: COX-2; Nephthea columnaris; antiinflammatory; cembrane; cytotoxicity; iNOS; octocoral