title: Silver nanoparticles synthesised using plant extracts show strong antibacterial activity.
abstract: In this study, three plants Populus alba, Hibiscus arboreus and Lantana camara were explored for the synthesis of silver nanoparticles (SNPs). The effect of reaction temperature and leaf extract (LE) concentration of P. alba, H. arboreus and L. camara was evaluated on the synthesis and size of SNPs. The SNPs were characterised by ultra-violet-visible spectroscopy, scanning electron microscopy and atomic force microscopy. The synthesis rate of SNPs was highest with LE of L. camara followed by H. arboreus and P. alba under similar conditions. L. camara LE showed maximum potential of smaller size SNPs synthesis, whereas bigger particles were formed by H. arboreous LE. The size and shape of L. camara LE synthesised SNPs were analysed by transmission electron microscopy (TEM) and energy dispersive X-ray spectroscopy (EDX). TEM analysis revealed the formation of SNPs of average size 17±9.5 nm with 5% LE of L. camara. The SNPs synthesised by LE of L. camara showed strong antibacterial activity against Escherichia coli. The results document that desired size SNPs can be synthesised using these plant LEs at a particular temperature for applications in the biomedical field.
authors.affiliation: Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176061, Himachal Pradesh, India.
authors.forename: Avnesh
authors.initials: A
authors.lastname: Kumari
authors.affiliation: Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176061, Himachal Pradesh, India.
authors.forename: Anika
authors.initials: A
authors.lastname: Guliani
authors.affiliation: Academy of Scientific and Innovative Research, New Delhi, India.
authors.forename: Rubbel
authors.initials: R
authors.lastname: Singla
authors.affiliation: Academy of Scientific and Innovative Research, New Delhi, India.
authors.forename: Ramdhan
authors.initials: R
authors.lastname: Yadav
authors.affiliation: Academy of Scientific and Innovative Research, New Delhi, India. sudeshkumar@ihbt.res.in.
authors.forename: Sudesh Kumar
authors.initials: SK
authors.lastname: Yadav
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D048430:Cell Shape; D004926:Escherichia coli / Q000187:drug effects; D031584:Hibiscus / Q000737:chemistry; D032410:Lantana / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D008853:Microscopy; D010316:Particle Size; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D032107:Populus / Q000737:chemistry; D012834:Silver / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D012834:Silver