title: Characterization of the Pall Celeris system as a point-of-care device for therapeutic angiogenesis.
abstract: OBJECTIVE
  The Pall Celeris system is a filtration-based point-of-care device designed to obtain a high concentrate of peripheral blood total nucleated cells (PB-TNCs). We have characterized the Pall Celeris-derived TNCs for their in vitro and in vivo angiogenic potency.
  
  
  METHODS
  PB-TNCs isolated from healthy donors were characterized through the use of flow cytometry and functional assays, aiming to assess migratory capacity, ability to form capillary-like structures, endothelial trans-differentiation and paracrine factor secretion. In a hind limb ischemia mouse model, we evaluated perfusion immediately and 7 days after surgery, along with capillary, arteriole and regenerative fiber density and local bio-distribution.
  
  
  RESULTS
  Human PB-TNCs isolated by use of the Pall Celeris filtration system were shown to secrete a panel of angiogenic factors and migrate in response to vascular endothelial growth factor and stromal-derived factor-1 stimuli. Moreover, after injection in a mouse model of hind limb ischemia, PB-TNCs induced neovascularization by increasing capillary, arteriole and regenerative fiber numbers, with human cells detected in murine tissue up to 7 days after ischemia.
  
  
  CONCLUSIONS
  The Pall Celeris system may represent a novel, effective and reliable point-of-care device to obtain a PB-derived cell product with adequate potency for therapeutic angiogenesis.
authors.affiliation: Vascular Biology and Regenerative Medicine Unit, Centro Cardiologico Monzino, IRCCS, Milan, Italy. Electronic address: gspaltro@ccfm.it.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Spaltro
authors.affiliation: Laboratory of Vascular Pathology, Istituto Dermopatico dell'Immacolata, IRCCS, Rome, Italy; Explora Biotech srl, Rome, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Straino
authors.affiliation: Vascular Biology and Regenerative Medicine Unit, Centro Cardiologico Monzino, IRCCS, Milan, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Gambini
authors.affiliation: Vascular Biology and Regenerative Medicine Unit, Centro Cardiologico Monzino, IRCCS, Milan, Italy.
authors.forename: Beatrice
authors.initials: B
authors.lastname: Bassetti
authors.affiliation: DIEC-Dipartimento di Economia, Università degli Studi di Genova, Genoa, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Persico
authors.affiliation: Department of Cardiovascular Surgery, University of Milan, Centro Cardiologico Monzino, IRCCS, Milan, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Zoli
authors.affiliation: Department of Cardiovascular Surgery, University of Milan, Centro Cardiologico Monzino, IRCCS, Milan, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Zanobini
authors.affiliation: Laboratory of Vascular Pathology, Istituto Dermopatico dell'Immacolata, IRCCS, Rome, Italy.
authors.forename: Maurizio C
authors.initials: MC
authors.lastname: Capogrossi
authors.affiliation: Department of Cardiovascular Surgery, University of Milan, Centro Cardiologico Monzino, IRCCS, Milan, Italy.
authors.forename: Rita
authors.initials: R
authors.lastname: Spirito
authors.affiliation: Medical Division, Pall Italia srl, Italy.
authors.forename: Cinzia
authors.initials: C
authors.lastname: Quarti
authors.affiliation: Vascular Biology and Regenerative Medicine Unit, Centro Cardiologico Monzino, IRCCS, Milan, Italy; Department of Clinical Sciences and Community Health, University of Milan, Milan, Italy.
authors.forename: Giulio
authors.initials: G
authors.lastname: Pompilio
mesh_terms: D000818:Animals; D001781:Blood Component Removal; D002454:Cell Differentiation; D002465:Cell Movement; D002469:Cell Separation / Q000379:methods; D054377:Chemokine CXCL12 / Q000378:metabolism; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000166:cytology; D005374:Filtration; D005434:Flow Cytometry; D006614:Hindlimb / Q000098:blood supply; D006801:Humans; D007511:Ischemia / Q000628:therapy*; D007962:Leukocytes / Q000276:immunology; D051379:Mice; D018919:Neovascularization, Physiologic; D058729:Peripheral Arterial Disease / Q000628:therapy*; D019095:Point-of-Care Systems; D015424:Reperfusion; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D054377:Chemokine CXCL12; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A
keywords: angiogenesis; hind limb ischemia; peripheral blood cells; point-of-care