title: An Antioxidant Extract of the Insectivorous Plant Drosera burmannii Vahl. Alleviates Iron-Induced Oxidative Stress and Hepatic Injury in Mice.
abstract: Free iron typically leads to the formation of excess free radicals, and additional iron deposition in the liver contributes to the oxidative pathologic processes of liver disease. Many pharmacological properties of the insectivorous plant Drosera burmannii Vahl. have been reported in previous studies; however, there is no evidence of its antioxidant or hepatoprotective potential against iron overload. The antioxidant activity of 70% methanolic extract of D. burmannii (DBME) was evaluated. DBME showed excellent DPPH, hydroxyl, hypochlorous, superoxide, singlet oxygen, nitric oxide, peroxynitrite radical and hydrogen peroxide scavenging activity. A substantial iron chelation (IC50 = 40.90 ± 0.31 μg/ml) and supercoiled DNA protection ([P]50 = 50.41 ± 0.55 μg) were observed. DBME also displayed excellent in vivo hepatoprotective activity in iron-overloaded Swiss albino mice compared to the standard desirox treatment. Administration of DBME significantly normalized serum enzyme levels and restored liver antioxidant enzymes levels. DBME lowered the raised levels of liver damage parameters, also reflected from the morphological analysis of the liver sections. DBME also reduced liver iron content by 115.90% which is also seen by Perls' staining. A phytochemical analysis of DBME confirms the presence of various phytoconstituents, including phenols, flavonoids, carbohydrates, tannins, alkaloids and ascorbic acid. Alkaloids, phenols and flavonoids were abundantly found in DBME. An HPLC analysis of DBME revealed the presence of purpurin, catechin, tannic acid, reserpine, methyl gallate and rutin. Purpurin, tannic acid, methyl gallate and rutin displayed excellent iron chelation but exhibited cytotoxicity toward normal (WI-38) cells; while DBME found to be non-toxic to the normal cells. These findings suggest that the constituents present in DBME contributed to its iron chelation activity. Additional studies are needed to determine if DBME can be used as a treatment for iron overload diseases.
authors.affiliation: Division of Molecular Medicine, Bose Institute, P 1/12, Scheme-VIIM, Kolkata, West Bengal, India.
authors.forename: Nikhil Baban
authors.initials: NB
authors.lastname: Ghate
authors.affiliation: Division of Molecular Medicine, Bose Institute, P 1/12, Scheme-VIIM, Kolkata, West Bengal, India.
authors.forename: Dipankar
authors.initials: D
authors.lastname: Chaudhuri
authors.affiliation: Division of Molecular Medicine, Bose Institute, P 1/12, Scheme-VIIM, Kolkata, West Bengal, India.
authors.forename: Abhishek
authors.initials: A
authors.lastname: Das
authors.affiliation: Division of Molecular Medicine, Bose Institute, P 1/12, Scheme-VIIM, Kolkata, West Bengal, India.
authors.forename: Sourav
authors.initials: S
authors.lastname: Panja
authors.affiliation: Division of Molecular Medicine, Bose Institute, P 1/12, Scheme-VIIM, Kolkata, West Bengal, India.
authors.forename: Nripendranath
authors.initials: N
authors.lastname: Mandal
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D029787:Drosera / Q000737:chemistry*; D007501:Iron / Q000009:adverse effects* / Q000494:pharmacology; D019190:Iron Overload / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D008099:Liver / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D007501:Iron