title: Therapeutic potential of an AcHERV-HPV L1 DNA vaccine.
abstract: Cervical cancer is strongly associated with chronic human papillomavirus infections, among which HPV16 is the most common. Two commercial HPV vaccines, Gardasil and Cervarix are effective for preventing HPV infection, but cannot be used to treat existing HPV infections. Previously, we developed a human endogenous retrovirus (HERV)-enveloped recombinant baculovirus capable of delivering the L1 genes of HPV types 16, 18, and 58 (AcHERV-HP16/18/58L1, AcHERV-HPV). Intramuscular administration of AcHERVHPV vaccines induced a strong cellular immune response as well as a humoral immune response. In this study, to examine the therapeutic effect of AcHERV-HPV in a mouse model, we established an HPV16 L1 expressing tumor cell line. Compared to Cervarix, immunization with AcHERVHPV greatly enhanced HPV16 L1-specific cytotoxic T lymphocytes (CTL) in C57BL/6 mice. Although vaccination could not remove preexisting tumors, strong CTL activity retarded the growth of inoculated tumor cells. These results indicate that AcHERV-HPV could serve as a potential therapeutic DNA vaccine against concurrent infection with HPV 16, 18, and 58.
authors.affiliation: Department of Bio-industrial Technologies, Konkuk University, Seoul, 143-701, Republic of Korea.
authors.forename: Hee-Jung
authors.initials: HJ
authors.lastname: Lee
authors.forename: Jong Kwang
authors.initials: JK
authors.lastname: Yoon
authors.forename: Yoonki
authors.initials: Y
authors.lastname: Heo
authors.forename: Hansam
authors.initials: H
authors.lastname: Cho
authors.forename: Yeondong
authors.initials: Y
authors.lastname: Cho
authors.forename: Yongdae
authors.initials: Y
authors.lastname: Gwon
authors.forename: Kang Chang
authors.initials: KC
authors.lastname: Kim
authors.forename: Jiwon
authors.initials: J
authors.lastname: Choi
authors.forename: Jae Sung
authors.initials: JS
authors.lastname: Lee
authors.forename: Yu-Kyoung
authors.initials: YK
authors.lastname: Oh
authors.forename: Young Bong
authors.initials: YB
authors.lastname: Kim
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology*; D002277:Carcinoma / Q000628:therapy*; D004195:Disease Models, Animal; D004337:Drug Carriers; D020077:Endogenous Retroviruses / Q000235:genetics*; D008810:Mice, Inbred C57BL; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000276:immunology*; D030361:Papillomavirus Infections / Q000628:therapy*; D053918:Papillomavirus Vaccines / Q000235:genetics / Q000276:immunology / Q000627:therapeutic use*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D016896:Treatment Outcome; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology / Q000627:therapeutic use*
chemical_list: D036022:Capsid Proteins; D004337:Drug Carriers; C114580:HPV L1 protein, Human papillomavirus; D009856:Oncogene Proteins, Viral; D053918:Papillomavirus Vaccines; D019444:Vaccines, DNA