title: Characterization of pKP-M1144, a Novel ColE1-Like Plasmid Encoding IMP-8, GES-5, and BEL-1 β-Lactamases, from a Klebsiella pneumoniae Sequence Type 252 Isolate.
abstract: IMP-8 metallo-β-lactamase was identified in Klebsiella pneumoniae sequence type 252 (ST252), isolated in a Portuguese hospital in 2009. blaIMP-8 was the first gene cassette of a novel class 3 integron, In1144, also carrying the blaGES-5, blaBEL-1, and aacA4 cassettes. In1144 was located on a ColE1-like plasmid, pKP-M1144 (12,029 bp), with a replication region of limited nucleotide similarity to those of other RNA-priming plasmids, such as pJHCMW1. In1144 and pKP-M1144 represent an interesting case of evolution of resistance determinants in Gram-negative bacteria.
authors.affiliation: Faculty of Medicine and University Hospital in Plzen, Charles University in Prague, Plzen, Czech Republic National Medicines Institute, Warsaw, Poland c.papagiannitsis@gmail.com.
authors.forename: Costas C
authors.identifier: http://orcid.org/0000-0002-9912-9028
authors.initials: CC
authors.lastname: Papagiannitsis
authors.affiliation: Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic CEITEC VFU, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic.
authors.forename: Monika
authors.initials: M
authors.lastname: Dolejska
authors.affiliation: National Medicines Institute, Warsaw, Poland.
authors.forename: Radoslaw
authors.initials: R
authors.lastname: Izdebski
authors.affiliation: Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic CEITEC VFU, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic.
authors.forename: Hana
authors.initials: H
authors.lastname: Dobiasova
authors.affiliation: Faculty of Medicine and University Hospital in Plzen, Charles University in Prague, Plzen, Czech Republic.
authors.forename: Vendula
authors.initials: V
authors.lastname: Studentova
authors.affiliation: Centro Hospitalar, Trás-os-Montes e Alto Douro, Vila Real, Portugal.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Esteves
authors.affiliation: University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Lennie P G
authors.initials: LP
authors.lastname: Derde
authors.affiliation: University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Marc J M
authors.initials: MJ
authors.lastname: Bonten
authors.affiliation: Faculty of Medicine and University Hospital in Plzen, Charles University in Prague, Plzen, Czech Republic Biomedical Center, Faculty of Medicine in Plzen, Charles University in Prague, Plzen, Czech Republic.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Hrabák
authors.affiliation: National Medicines Institute, Warsaw, Poland.
authors.forename: Marek
authors.initials: M
authors.lastname: Gniadkowski
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D015780:Carbapenems / Q000494:pharmacology; D004269:DNA, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D006801:Humans; D007710:Klebsiella Infections / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010957:Plasmids / Q000187:drug effects / Q000235:genetics*; D011174:Portugal; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D004269:DNA, Bacterial; C436760:IMP-8 enzyme; D001618:beta-Lactamases; C000607156:beta-lactamase BEL-1, Klebsiella pneumoniae; C000607155:beta-lactamase GES-5, Klebsiella pneumoniae; C063912:carbapenemase