title: Dynamical formation of lipid bilayer vesicles from lipid-coated droplets across a planar monolayer at an oil/water interface.
abstract: Recently, the transfer method has been shown to be useful for preparing cell-sized phospholipid bilayer vesicles, within which desired substances at desired concentrations can be encapsulated, with a desired asymmetric lipid composition. Here, we investigated the transfer process of water-in-oil (W/O) droplets coated by phospholipid monolayers across an oil/water interface by both experimental observation and theoretical modeling. Real-time experimental observation of the transfer revealed that the transfer process is characterized by three kinetic regimes: a precontact process (approaching regime), an early fast process (entering regime), and a late slow process (relaxation regime). In addition, bigger droplets require much more time to transfer than smaller droplets. We propose a theoretical model to interpret this kinetic process. Our theoretical model reproduces the essential aspects of the transfer kinetics, including its size-dependence.
authors.affiliation: Department of Physics, Graduate School of Science, Kyoto University, Kyoto, 606-8502, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ito
authors.forename: Toru
authors.initials: T
authors.lastname: Yamanaka
authors.forename: Shou
authors.initials: S
authors.lastname: Kato
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Hamada
authors.forename: Masahiro
authors.initials: M
authors.lastname: Takagi
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Ichikawa
authors.forename: Kenichi
authors.initials: K
authors.lastname: Yoshikawa
mesh_terms: D007700:Kinetics; D008051:Lipid Bilayers / Q000737:chemistry*; D009821:Oils / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*; D053835:Unilamellar Liposomes / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D008051:Lipid Bilayers; D009821:Oils; D010743:Phospholipids; D053835:Unilamellar Liposomes; D014867:Water