title: Library Construction for High-Throughput Mobile Element Identification and Genotyping.
abstract: Mobile genetic elements are discrete DNA elements that can move around and copy themselves in a genome. As a ubiquitous component of the genome, mobile elements contribute to both genetic and epigenetic variation. Therefore, it is important to determine the genome-wide distribution of mobile elements. Here we present a targeted high-throughput sequencing protocol called Mobile Element Scanning (ME-Scan) for genome-wide mobile element detection. We will describe oligonucleotides design, sequencing library construction, and computational analysis for the ME-Scan protocol.
authors.affiliation: Department of Genetics, Rutgers, the State University of New Jersey, Piscataway, NJ, USA.|Human Genetic Institute of New Jersey, Rutgers, the State University of New Jersey, Piscataway, NJ, USA.
authors.forename: Hongseok
authors.initials: H
authors.lastname: Ha
authors.affiliation: Department of Genetics, Rutgers, the State University of New Jersey, Piscataway, NJ, USA.|Human Genetic Institute of New Jersey, Rutgers, the State University of New Jersey, Piscataway, NJ, USA.
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.affiliation: Department of Genetics, Rutgers, the State University of New Jersey, Piscataway, NJ, USA. xing@biology.rutgers.edu.|Human Genetic Institute of New Jersey, Rutgers, the State University of New Jersey, Piscataway, NJ, USA. xing@biology.rutgers.edu.
authors.forename: Jinchuan
authors.initials: J
authors.lastname: Xing
mesh_terms: D019295:Computational Biology / Q000379:methods*; D015894:Genome, Human; D015698:Genomic Library; D005838:Genotype; D060005:Genotyping Techniques / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D020071:Interspersed Repetitive Sequences; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA / Q000379:methods*
keywords: High-throughput sequencing; ME-Scan; Mobile element; Polymorphism; Population diversity