title: Chinese medicinal formula Fufang Xueshuantong capsule could inhibit the activity of angiotensin converting enzyme.
abstract: Fufang Xueshuantong (FXST) capsule, a Chinese medicinal formula composed of four herbals -Panax notoginseng,Radix Astragali,Radix Salvia MiltiorrhizaeandRadix Scrophulariaceae, has been used to treat cardiovascular diseases for many years, but the pharmacological mechanisms underlying its effects has not been clarified. This study investigates if a connection between FXST and angiotensin converting enzyme (ACE) might be an explanation for its pharmacological effects. ACE inhibition assay was performed on FXST capsule, 50% ethanol extracts from the four herbals and three selected saponins most abundant inP. notoginseng(Ginsenoside Rg1, Ginsenoside Rb1 and Notoginsenoside R1) using a biochemical test. Reversed-phase high-performance liquid chromatography of liberated hippuric acid from the ACE assay was conducted to determine the inhibitory effect. As a result, FXST and extracts fromP. notoginsengshowed a significant and dose-dependent inhibition on ACE activity with the IC50values of 115 μg/ml and 179 μg/ml, respectively. But extracts from the other three herbals and the three selected saponins had no significant effect on ACE inhibition. Compared to other reported plant extracts, FXST could be considered as an effective ACE inhibitor. The inhibition of ACE activity supports the traditional use of FXST on blood circulation and the inhibitory property of FXST is mainly caused byP. notoginseng.
authors.affiliation: Guangzhou Quality R&D Center of Traditional Chinese Medicine, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University , Guangzhou , PR China ; Guangdong Zhongsheng Pharmaceutical Co., Ltd. , Dongguan , PR China.
authors.forename: Shujing
authors.initials: S
authors.lastname: Sheng
authors.affiliation: Guangzhou Quality R&D Center of Traditional Chinese Medicine, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University , Guangzhou , PR China.
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Guangdong Zhongsheng Pharmaceutical Co., Ltd. , Dongguan , PR China.
authors.forename: Chaofeng
authors.initials: C
authors.lastname: Long
authors.affiliation: Guangzhou Quality R&D Center of Traditional Chinese Medicine, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University , Guangzhou , PR China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Su
authors.affiliation: Guangzhou Blood Center , Guangzhou , 510095 , China.
authors.forename: Xia
authors.initials: X
authors.lastname: Rong
keywords: Fufang Xueshuantong capsule; Panax notoginseng; angiotensin converting enzyme; saponins