title: Evaluation of antioxidant producing potential of halophilic bacterial bionts from marine invertebrates.
abstract: Marine invertebrates exposed to high levels of reactive oxygen species in the oceans have been reported to produce antioxidants as a major defense against free radical mediated toxicity; protecting their tissues from the damage associated with the oxidative stress. In view of this, the present study was carried out to determine the antioxidant activity of 100 bacterial bionts isolated from marine sponges, corals and a single bivalve. Methanol extract of biont GUVFCFM-3 produced 67.83% scavenging of 2,2-diphenyl-2-picrylhydrazyl free radicals and 65.87% scavenging of superoxide free radicals. Preliminary tests leading to the identification of the extracellular antioxidant factor produced by GUVFCFM-3 revealed that it is a peptide. We report that the genera Chromohalobacter sp. primarily known for its unique salt tolerating abilities by virtue of the production of osmolytes is an excellent scavenger of free radicals.
authors.affiliation: Department of Microbiology, Goa University, Taleigao Plateau, Goa-403 203, India.
authors.forename: Sheryanne
authors.initials: S
authors.lastname: Velho-Pereira
authors.affiliation: Department of Microbiology, Goa University, Taleigao Plateau, Goa-403 203, India.
authors.forename: P
authors.initials: P
authors.lastname: Parvatkar
authors.affiliation: Department of Microbiology, Goa University, Taleigao Plateau, Goa-403 203, India.
authors.forename: Irene J
authors.initials: IJ
authors.lastname: Furtado
keywords: Antioxidant; Chromohalobacter sp.; bacterial bionts; invertebrates