title: Growth characteristics and expression of CD73 and CD146 in cells cultured from dental pulp.
abstract: OBJECTIVE
  Dental pulp stem cells (DPSCs) in permanent teeth and stem cells from human exfoliated deciduous (SHED) teeth are unique sources of mesenchymal stem cells. The aim of this study was to compare the growth characteristics and morphology of DPSCs and SHED and their immuno-phenotype using CD73 and CD146 in the 1st, 3rd, and 5th passage of cell culture.
  
  
  METHODS
  Growth characteristics, morphology, and colony forming efficiency were assessed for SHED and DPSCs. Immunocytochemistry and flow cytometry using CD146 and CD73 was performed for SHED and DPSCs in the 1st, 3rd and 5th passage of culture. Data was analyzed using SPSS™ software (version 17.0.0).
  
  
  RESULTS
  The seeding efficiency and colony forming unit efficiency was higher in SHED than in DPSCs. Flow cytometry analysis using CD73 and CD146 showed an increase in CD73 expression with increase in passage number in SHED and a decrease in CD73 expression with increase in passage number in DPSCs. There was a decrease in CD146 expression from passage one through five in SHED and DPSCs.
  
  
  CONCLUSIONS
  Cells isolated from the pulp of deciduous teeth and permanent teeth show difference in their growth characteristics and phenotype and are a viable source of stem cells.
authors.affiliation: Department of Oral and Maxillofacial Pathology, Ragas Dental College and Hospital, Chennai, Tamil Nadu, India.
authors.forename: Vaishnavi
authors.initials: V
authors.lastname: Sivasankar
authors.affiliation: Department of Oral and Maxillofacial Pathology, Ragas Dental College and Hospital, Chennai, Tamil Nadu, India.
authors.forename: Kannan
authors.initials: K
authors.lastname: Ranganathan
mesh_terms: D015720:5'-Nucleotidase / Q000096:biosynthesis*; D051929:CD146 Antigen / Q000096:biosynthesis; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology*; D005347:Fibroblasts / Q000378:metabolism; D005434:Flow Cytometry; D058851:GPI-Linked Proteins / Q000096:biosynthesis; D006801:Humans; D016130:Immunophenotyping; D059630:Mesenchymal Stem Cells / Q000276:immunology / Q000378:metabolism*; D008938:Mitosis
chemical_list: D051929:CD146 Antigen; D058851:GPI-Linked Proteins; C497902:MCAM protein, human; D015720:5'-Nucleotidase; C548241:NT5E protein, human
keywords: dental pulp; dental pulp stem cells; immunocytochemistry; mesenchymal stem cells; stem cells from human exfoliated deciduous