title: Design and synthesis of pH-sensitive polyamino-ester magneto-dendrimers: Surface functional groups effect on viability of human prostate carcinoma cell lines DU145.
abstract: Novel pH-sensitive, biocompatible and biodegradable magneto-dendrimers with OH and/or NH2 functional groups based on poly amino-ester were synthesized for delivery of anti-cancer drugs. Magnetite nanoparticles (MNPs) were synthesized by the co-precipitation method and their surfaces were modified by 3-aminopropyl triethoxysilane. The first and second generations of the magneto-dendrimer with hydroxyl end groups were produced by sequential acrylation and Michael addition reactions using the required amounts of acryloyl chloride and diethanolamine, respectively. The dendrimer containing amino functional surface groups up to second generation was synthesized by the same method using the necessary amounts of acryloyl chloride and ethylenediamine. These dendrimers were fully characterized by the Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), dynamic light scattering (DLS) and zeta potential analysis, vibrating-sample magnetometer (VSM), scanning electron microscope (SEM), transmission electron microscopy (TEM) and selected area electron diffraction (SAED). In-vitro release profiles of the drug-loaded magnetic nanoparticles and their cytotoxicity assay were investigated at two pHs (7.4 and 5.8). The hydrolytic degradation behavior of magneto-dendrimers was evaluated in PBS buffer. Our research suggests that magneto-dendrimers having amine or hydroxyl functional groups could be considered as the suitable nanocarriers for therapy applications.
authors.affiliation: Department of Chemistry, University of Zanjan, P.O. Box 45195-313, Zanjan, Iran.
authors.forename: Nahid
authors.initials: N
authors.lastname: Dayyani
authors.affiliation: Polymer Laboratory, Chemistry Department, School of Science, University of Tehran, P.O. Box 14155-6455, Tehran, Iran. Electronic address: Khoee@khayam.ut.ac.ir.
authors.forename: Sepideh
authors.initials: S
authors.lastname: Khoee
authors.affiliation: Department of Chemistry, University of Zanjan, P.O. Box 45195-313, Zanjan, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Ramazani
mesh_terms: D045744:Cell Line, Tumor; D050091:Dendrimers / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D015195:Drug Design; D004952:Esters / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008280:Magnetics; D008297:Male; D008854:Microscopy, Electron; D011073:Polyamines / Q000737:chemistry; D011471:Prostatic Neoplasms / Q000473:pathology*; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry
chemical_list: D050091:Dendrimers; D004952:Esters; D011073:Polyamines
keywords: Cytotoxicity assay; Drug nanocarrier; Magneto-dendrimer; Polyamino-ester; pH-responsive