title: [Study of the relationship among expression of Survivin and MRP and the drug resistance in human nasopharyngeal carcinoma].
abstract: OBJECTIVE
  This study aimed to explore the relationship among expression of Survivin and MRP and drug resistance in NPC.
  
  
  METHODS
  Expression of Survivin were detected by immunohistochemistry method in 45 cases of NPC and 24 cases of normal mucous membrane of nasopharynx (NMMN). The relationship between expression of Survivin and pathological factors in NPC were analysized. Expression of Survivin and MRP were detected in 31 patients of NPC with paclitaxel resistance and 20 patients of NPC without paclitaxel resistance. The relation- ship among the expression of Survivin or MRP and paclitaxel resistance in NPC were analysized. The paclitaxel resistance cell line, 5-8F-PTX(+); was established by a step-increased method. The expression of Survivin and MRP were detected by western blot in 5-8F-PTX(+) and 5-8F.
  
  
  RESULTS
  The positive were 71. 1% (32/45) in NPC and 8.33% (2/24) in NMMN. And there were significantly differences between them (P < .05). There were relationship among expression of Survivin and differentiation degree, lymph node metastasis, distant metastasis, and clinic stages of NPC. The positive were 75.9% (31/39) in moderately differentiated NPC and 16.7% (1/6) in lowly differentiated NPC, respectively. There were significantly differences between them (P < 0.05). The positive of Survivin were 83.9% (26/31) in NPC patients with paclitaxel resistance and 45.0% (9/20) in NPC patients without Paclitaxel resistance, respectively. There were significantly differences between them (P < 0.05). The positive of MRP were 87.1% (27/31) in NPC patients with paclitaxel resistance and 40.0% (8/20) in NPC patients without paclitaxel resistance, respectively. There were significantly differences between them (P < 0.05). There were positive correlation between the expression of Survivin and MRP in NPC patients with Paclitaxel resistance. The expression of Survivin and MRP were higher in 5-8F-PTX(+) than in 5-8F. The IC50 of paclitaxel, cDDP, 5-FU and Vincristine were significantly higher in 5-8F-PTX(+) than in 5-8F.
  
  
  CONCLUSIONS
  There were relationship among the expression of Survivin and difference, metastasis and TNM stages of NPC. Survivin may serves as a molecular marker for development and progress in NPC. There were relationship among the high expression of Survivin and MRP and increasing of drug resistance in NPC.
authors.forename: Ning
authors.initials: N
authors.lastname: Yang
authors.forename: Lepan
authors.initials: L
authors.lastname: Zhu
authors.forename: Tan
authors.initials: T
authors.lastname: Tan
authors.forename: Chunyan
authors.initials: C
authors.lastname: Hou
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D002277:Carcinoma; D002945:Cisplatin; D019008:Drug Resistance, Neoplasm; D005472:Fluorouracil; D006801:Humans; D007150:Immunohistochemistry; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics / Q000378:metabolism*; D008207:Lymphatic Metastasis; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000235:genetics / Q000378:metabolism*; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000378:metabolism*; D009305:Nasopharynx / Q000378:metabolism; D017239:Paclitaxel / Q000494:pharmacology; D000077022:Survivin; D014750:Vincristine
chemical_list: D000970:Antineoplastic Agents; D002945:Cisplatin; D005472:Fluorouracil; D051034:Inhibitor of Apoptosis Proteins; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D017239:Paclitaxel; D000077022:Survivin; D014750:Vincristine; C107489:BIRC5 protein, human