title: Impact of surfactant assisted acid and alkali pretreatment on lignocellulosic structure of pine foliage and optimization of its saccharification parameters using response surface methodology.
abstract: In present study, two hybrid methods such as surfactant assisted acid pretreatment (SAAP) and surfactant assisted base pretreatment (SABP) of pine foliage (PF) were found efficient for removal of 59.53 ± 0.76% and 73.47 ± 1.03% lignin, respectively. Assessment of the impact of pretreatment over the structure of PF were studied by scanning electron microscopy, Fourier transform infrared and X-ray diffraction analysis. Parameters for saccharification of SAAP and SABP biomass were optimized by Box-Behnken design method and 0.588 g/g and 0.477 g/g of reducing sugars were obtained, respectively. The ethanol fermentation efficiency of Saccharomyces cerevisiae (NCIM 3288) of hydrolysates was increased by 16.1% and 6.01% in SAAP-PFF and SABP-PFF after detoxification with XAD-4 resin. The mass balance analysis of the process showed that 67.7% and 70.12% cellulose were utilized during SAAP and SABP, respectively. These results indicated that SAAP would be more economic for bioethanol production.
authors.affiliation: Department of Biotechnology, Motilal Nehru National Institute of Technology, Allahabad 211004, Uttar Pradesh, India.
authors.forename: Ajay Kumar
authors.initials: AK
authors.lastname: Pandey
authors.affiliation: Department of Biotechnology, Motilal Nehru National Institute of Technology, Allahabad 211004, Uttar Pradesh, India. Electronic address: sn5@mnnit.ac.in.
authors.forename: Sangeeta
authors.initials: S
authors.lastname: Negi
mesh_terms: D000143:Acids / Q000737:chemistry*; D000468:Alkalies; D018533:Biomass; D002241:Carbohydrates / Q000737:chemistry; D002482:Cellulose / Q000737:chemistry; D000431:Ethanol / Q000737:chemistry; D005285:Fermentation / Q000502:physiology; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning / Q000379:methods; D028223:Pinus / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry; D011145:Polyvinyls / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000502:physiology; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013501:Surface-Active Agents / Q000737:chemistry*; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: D000143:Acids; D000468:Alkalies; D002241:Carbohydrates; D011137:Polystyrenes; D011145:Polyvinyls; D013501:Surface-Active Agents; C036909:lignocellulose; C012101:XAD-4 resin; D000431:Ethanol; D002482:Cellulose; D008031:Lignin
keywords: Mass balance; Pine fallen foliages; Pretreatment; Reducing sugar yield; Saccharification