title: First record of a large-scale bloom-causing species Nannochloropsis granulata (Monodopsidaceae, Eustigmatophyceae) in China Sea waters.
abstract: Nannochloropsis is an ubiquitous genus, found in diverse aquatic environments including open ocean as well as fresh and brackish water. Recently, large-scale blooms occurred frequently along eutrophic coastal zone from the Bohai Sea to the northern Yellow Sea in China. The cell density reached 10(9) to 10(10)cells per liter during a bloom near Qinhuangdao, Hebei Province. The bloom forming species, a yellow-green microalgae was successfully isolated and cultivated in the laboratory. Microscopic observation indicated that the cells contained simple morphological characteristics with a diameter about 2 μm. Pigment analyses confirmed that the pigment composition of the newly isolated strain BDH02 was similar to that of Nannochloropsis granulata. Phylogenetic analyses of 18S rRNA gene, ITS, and rbcL gene indicated that the strain was closely related to N. granulata. This is the first record of a bloom caused by N. granulata in China.
authors.affiliation: College of Marine Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, China.|Tianjin Key Laboratory of Marine Resources and Chemistry, Tianjin, 300457, People's Republic of China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Microbiology Division, Stroud Water Research Center, Avondale, PA, 19311, USA.
authors.forename: Jinjun
authors.initials: J
authors.lastname: Kan
authors.affiliation: College of Marine Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, China.|Tianjin Key Laboratory of Marine Resources and Chemistry, Tianjin, 300457, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Third Institute of Oceanography, State Oceanic Administration, Xiamen, 361005, China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Gu
authors.affiliation: State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai, 200062, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Hu
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: College of Marine Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, China. phytoplankton@163.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Sun
mesh_terms: D020418:Algal Proteins / Q000235:genetics / Q000378:metabolism; D002681:China; D048028:DNA, Algal / Q000235:genetics / Q000378:metabolism; D021901:DNA, Intergenic / Q000235:genetics / Q000378:metabolism; D005068:Eutrophication; D058086:Microalgae / Q000235:genetics / Q000254:growth & development*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics / Q000378:metabolism; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics / Q000378:metabolism; D012623:Seawater / Q000032:analysis; D017422:Sequence Analysis, DNA; D058009:Stramenopiles / Q000235:genetics / Q000254:growth & development*
chemical_list: D020418:Algal Proteins; D048028:DNA, Algal; D021901:DNA, Intergenic; D012337:RNA, Ribosomal, 18S; C101625:RbcL protein, plastid; D012273:Ribulose-Bisphosphate Carboxylase
keywords: 18S rRNA gene; Bloom forming species; China Seas; ITS; Morphology; Nannochloropsis granulata; rbcL gene