title: Transcriptomic Analysis Reveals Wound Healing of Morus alba Root Extract by Up-Regulating Keratin Filament and CXCL12/CXCR4 Signaling.
abstract: Facilitation of the wound healing process is important because a prolonged wound site increases pain and the risk of infection. In oriental medicine, an extract of Morus alba root (MA) has usually been prescribed as traditional treatment for accelerating wound healing, and it has been proven to be safe for centuries. To study the molecular mechanism of MA-mediated skin wound healing, we performed a primary cell culture and a skin explant culture and observed significant difference between the groups with and without MA extract. In the cellular system, a real-time cell analysis and real-time quantitative PCR were performed. It was found that MA extract enhanced proliferation in a dose-dependent manner on Kera-308 cell line, and up-regulated keratin expression including wound-induced Krt6a. In skin explant culture, the mRNA level derived from cell outgrowth displayed a tendency toward more up-regulated mRNA associated keratin filaments and toward a more up-regulated mRNA level of C-X-C motif chemokine 12 (CXCL12) and a chemokine receptor 4 (CXCR4) axis signaling pathway downstream. In this process, we concluded that MA extract had a scientific possibility of wound repair by increasing intracellular and extracellular supports and by inducing a CXCL12/CXCR4 signaling pathway.
authors.affiliation: College of Korean Medicine, Institute of Korean Medicine, Kyung Hee University, Heogi-dong, Dongdaemun-gu, Seoul, 130-701, Republic of Korea.
authors.forename: Kang-Hoon
authors.initials: KH
authors.lastname: Kim
authors.affiliation: College of Korean Medicine, Institute of Korean Medicine, Kyung Hee University, Heogi-dong, Dongdaemun-gu, Seoul, 130-701, Republic of Korea.
authors.forename: Won-Seok
authors.initials: WS
authors.lastname: Chung
authors.affiliation: College of Korean Medicine, Institute of Korean Medicine, Kyung Hee University, Heogi-dong, Dongdaemun-gu, Seoul, 130-701, Republic of Korea.|Western Seoul Center, Korea Basic Science Institute, 150 Bugahyeon-ro, Seodaemun-gu, Seoul, 120-140, Republic of Korea.
authors.forename: Yoomi
authors.initials: Y
authors.lastname: Kim
authors.affiliation: College of Korean Medicine, Institute of Korean Medicine, Kyung Hee University, Heogi-dong, Dongdaemun-gu, Seoul, 130-701, Republic of Korea.
authors.forename: Ki-Suk
authors.initials: KS
authors.lastname: Kim
authors.affiliation: College of Korean Medicine, Institute of Korean Medicine, Kyung Hee University, Heogi-dong, Dongdaemun-gu, Seoul, 130-701, Republic of Korea.
authors.forename: In-Seung
authors.initials: IS
authors.lastname: Lee
authors.affiliation: College of Korean Medicine, Institute of Korean Medicine, Kyung Hee University, Heogi-dong, Dongdaemun-gu, Seoul, 130-701, Republic of Korea.
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Park
authors.affiliation: College of Korean Medicine, Institute of Korean Medicine, Kyung Hee University, Heogi-dong, Dongdaemun-gu, Seoul, 130-701, Republic of Korea.
authors.forename: Hyeon-Soo
authors.initials: HS
authors.lastname: Jeong
authors.affiliation: Western Seoul Center, Korea Basic Science Institute, 150 Bugahyeon-ro, Seodaemun-gu, Seoul, 120-140, Republic of Korea.
authors.forename: Yun-Cheol
authors.initials: YC
authors.lastname: Na
authors.affiliation: School of Undergraduate Studies, College of Transdisciplinary Studies, Daegu Gyeongbuk Institute of Science and Technology, Daegu, Republic of Korea.
authors.forename: Chang-Hun
authors.initials: CH
authors.lastname: Lee
authors.affiliation: College of Korean Medicine, Institute of Korean Medicine, Kyung Hee University, Heogi-dong, Dongdaemun-gu, Seoul, 130-701, Republic of Korea.
authors.forename: Hyeung-Jin
authors.initials: HJ
authors.lastname: Jang
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D054377:Chemokine CXCL12 / Q000378:metabolism*; D066298:In Vitro Techniques; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism; D007633:Keratins / Q000378:metabolism*; D008813:Mice, Inbred ICR; D031625:Morus / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D061251:Primary Cell Culture; D012333:RNA, Messenger / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000378:metabolism*; D015398:Signal Transduction; D012867:Skin / Q000166:cytology / Q000187:drug effects; D059467:Transcriptome; D015854:Up-Regulation; D014945:Wound Healing / Q000187:drug effects*
chemical_list: C514936:CXCR4 protein, mouse; D054377:Chemokine CXCL12; C515256:Cxcl12 protein, mouse; D010936:Plant Extracts; D012333:RNA, Messenger; D019718:Receptors, CXCR4; D007633:Keratins
keywords: C-X-C motif chemokine 12 (CXCL12); Morus alba root; chemokine receptor 4 (CXCR4); therapeutic treatment