title: Analyses of in vivo interactions between transcription factors and the archaeal RNA polymerase.
abstract: Transcription factors regulate the activities of RNA polymerase (RNAP) at each stage of the transcription cycle. Many basal transcription factors with common ancestry are employed in eukaryotic and archaeal systems that directly bind to RNAP and influence intramolecular movements of RNAP and modulate DNA or RNA interactions. We describe and employ a flexible methodology to directly probe and quantify the binding of transcription factors to RNAP in vivo. We demonstrate that binding of the conserved and essential archaeal transcription factor TFE to the archaeal RNAP is directed, in part, by interactions with the RpoE subunit of RNAP. As the surfaces involved are conserved in many eukaryotic and archaeal systems, the identified TFE-RNAP interactions are likely conserved in archaeal-eukaryal systems and represent an important point of contact that can influence the efficiency of transcription initiation.
authors.affiliation: Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523, USA. Electronic address: julie.walker@colostate.edu.
authors.forename: Julie E
authors.initials: JE
authors.lastname: Walker
authors.affiliation: Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523, USA. Electronic address: thomas.santangelo@colostate.edu.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Santangelo
mesh_terms: D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000235:genetics*; D010957:Plasmids; D011487:Protein Conformation; D025941:Protein Interaction Mapping / Q000379:methods; D021122:Protein Subunits / Q000737:chemistry* / Q000235:genetics; D013329:Structure-Activity Relationship; D019712:Thermococcus / Q000235:genetics; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D021122:Protein Subunits; D014157:Transcription Factors; D012321:DNA-Directed RNA Polymerases
keywords: Archaea; RNA polymerase; TFE; Transcription; Transcription factor