title: Chitotriosidase Expression during Monocyte-Derived Dendritic Cells Differentiation and Maturation.
abstract: The chitotriosidase (CHIT-1) is a glycosyl hydrolase (GH), which has been found highly expressed in activated macrophages and in different monocyte-derived cell lines such as Kupffer cells and osteoclasts, as well is differently produced in diverse stages of macrophage polarization (M1 and M2). Recent finding suggests that CHIT-1 plays a crucial role in innate and acquired immunity. Dendritic cells (DCs) are a complex group of cells that play a critical role in immune response. The aim of this study was to investigate the presence of CHIT-1 during the differentiation and maturation of DCs. Magnetically-isolated peripheral blood monocytes were differentiated toward immature DCs (iDC) and mature DCs (mDCs). Our results showed, for the first time, that CHIT-1 is expressed during the process of differentiation and maturation of DCs in a time-dependent manner. We found that CHIT1 is evenly distributed in cytoplasm of both the iDCs and mDCs. Additionally, a significantly increased expression of CHIT1 mRNA and protein was observed in mature DCs. These results suggest that CHIT-1 play an important role in the DCs immunoresponse.
authors.affiliation: Department of Bio-medical and Biotechnological Sciences, University of Catania, Catania, Italy. mdirosa@unict.it.
authors.forename: Michelino
authors.initials: M
authors.lastname: Di Rosa
authors.affiliation: Department of Clinical and Molecular Biomedicine, University of Catania, Ospedale Ferrarotto, Italy.
authors.forename: Daniele
authors.initials: D
authors.lastname: Tibullo
authors.affiliation: Department of Bio-medical and Biotechnological Sciences, University of Catania, Catania, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Cambria
authors.affiliation: Department of Bio-medical and Biotechnological Sciences, University of Catania, Catania, Italy.
authors.forename: Gisella
authors.initials: G
authors.lastname: Distefano
authors.affiliation: Department of Biological, Geological and Environmental Sciences, University of Catania, Catania, Italy.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Saccone
authors.affiliation: Department of Clinical and Molecular Biomedicine, University of Catania, Ospedale Ferrarotto, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Di Raimondo
authors.affiliation: Department of Bio-medical and Biotechnological Sciences, University of Catania, Catania, Italy. lucmal@unict.it.
authors.forename: Lucia
authors.initials: L
authors.lastname: Malaguarnera
mesh_terms: D002454:Cell Differentiation; D002478:Cells, Cultured; D003593:Cytoplasm / Q000201:enzymology; D003713:Dendritic Cells / Q000201:enzymology* / Q000276:immunology; D015971:Gene Expression Regulation, Enzymologic; D006596:Hexosaminidases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D009000:Monocytes / Q000201:enzymology* / Q000276:immunology; D010641:Phenotype; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction; D013997:Time Factors
chemical_list: D012333:RNA, Messenger; D006596:Hexosaminidases; C086400:chitotriosidase
keywords: chitotriosidase; immunity; monocyte derived dendritic cells