title: Buffalo (Bubalus bubalis) term amniotic-membrane-derived cells exhibited mesenchymal stem cells characteristics in vitro.
abstract: Recent studies suggested that placentae amniotic membrane is a valuable source of stem cells in human as well as in livestock species. Advantages of amnion over other sources of stem cells included abundant availability, ethically non-objectionable and non-invasive source. The aim of the present study was the isolation, culture and characterization of amniotic-membrane-derived mesenchymal stem cells from term placentae collected postpartum in buffalo. We have observed that both presumptive epithelial-like and fibroblast-like cells were cultured and maintained from term amnion. These cells were shown the positive expression of pluripotency markers (OCT-4, SOX-2, NANOG, TERT), mesenchymal stem cell markers (CD29, CD44, CD105) and negative for haematopoietic marker (CD34) genes at different passages. In addition, these cells were also positive for alkaline phosphatase staining. Stem-ness potential of any stem cells is determined by their potential to differentiate into specific lineages of cell type. In the present study, we have successfully differentiated the amniotic-membrane-derived cells into adipogenic, chondrogenic and osteogenic lineages of cells in vitro. In conclusion, the results of this study demonstrate that amniotic-membrane-derived cells expressed pluripotent and mesenchymal stem cells markers and have propensity to differentiate into cells of mesenchymal lineage cell type upon directed differentiation in vitro.
authors.affiliation: Animal Physiology and Reproduction Division, Central Institute for Research on Buffaloes, Hisar, 125001, Haryana, India.|Department of Biotechnology, Chaudhary Devi Lal University, Sirsa, 125055, Haryana, India.
authors.forename: Kaushalya
authors.initials: K
authors.lastname: Ghosh
authors.affiliation: National Research Centre on Equines, Hisar, 125001, Haryana, India.
authors.forename: Rajesh
authors.initials: R
authors.lastname: Kumar
authors.affiliation: National Research Centre on Equines, Hisar, 125001, Haryana, India.
authors.forename: Jarnail
authors.initials: J
authors.lastname: Singh
authors.affiliation: Department of Biotechnology, Chaudhary Devi Lal University, Sirsa, 125055, Haryana, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Gahlawat
authors.affiliation: Animal Physiology and Reproduction Division, Central Institute for Research on Buffaloes, Hisar, 125001, Haryana, India.
authors.forename: Dharmendra
authors.initials: D
authors.lastname: Kumar
authors.affiliation: Animal Physiology and Reproduction Division, Central Institute for Research on Buffaloes, Hisar, 125001, Haryana, India.
authors.forename: Naresh Lalaji
authors.initials: NL
authors.lastname: Selokar
authors.affiliation: Animal Physiology and Reproduction Division, Central Institute for Research on Buffaloes, Hisar, 125001, Haryana, India.
authors.forename: S P
authors.initials: SP
authors.lastname: Yadav
authors.affiliation: National Research Centre on Equines, Hisar, 125001, Haryana, India.
authors.forename: B R
authors.initials: BR
authors.lastname: Gulati
authors.affiliation: Animal Physiology and Reproduction Division, Central Institute for Research on Buffaloes, Hisar, 125001, Haryana, India. psyadav@scientist.com.
authors.forename: P S
authors.initials: PS
authors.lastname: Yadav
mesh_terms: D050156:Adipogenesis; D000650:Amnion / Q000166:cytology*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002020:Buffaloes; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002478:Cells, Cultured; D005260:Female; D018960:Hyaluronan Receptors / Q000235:genetics; D019012:Integrin beta1 / Q000235:genetics; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D050814:Octamer Transcription Factor-3 / Q000235:genetics; D010012:Osteogenesis; D055748:SOXB1 Transcription Factors / Q000235:genetics; D019098:Telomerase / Q000235:genetics
chemical_list: D015415:Biomarkers; D018960:Hyaluronan Receptors; D019012:Integrin beta1; D050814:Octamer Transcription Factor-3; D055748:SOXB1 Transcription Factors; D019098:Telomerase
keywords: Amnion; Buffalo; Differentiation; Stem cell