title: Lipoproteins from Clostridium perfringens and their protective efficacy in mouse model.
abstract: Clostridium perfringens is an obligately anaerobic rod-shaped bacterium and etiological agent for several diseases in humans and animals. The pathogen has been listed as Validated Biological Agent and warrants development of medical countermeasures. The homologs of some of the lipoproteins identified from various fractions of C. perfringens in our previous studies were observed to be virulence determinants in other pathogenic bacteria. Three putative virulence associated lipoproteins; polysaccharide deacetylase family protein, probable ion-uptake ABC transporter, and a putative lipoprotein of no known function are reported here with respect to their immuno-protective potentials. The three proteins were over expressed and purified to near homogeneity. The lipoproteins were shown to be exposed on the C. perfringens surface and, hence, accessible to antibodies and potentially visible to the host immune system. Immunization of mice with purified recombinant proteins elicited protective immunity against challenge with C. perfringens in mouse gas gangrene model. Distribution and relationship of orthologous proteins across other bacterial select agents especially among the members of Firmicutes, was carried out to look for conserved antigenic determinants.
authors.affiliation: Biotechnology Division, Defence Research and Development Establishment, Gwalior 474002, India.
authors.forename: Pratistha
authors.initials: P
authors.lastname: Dwivedi
authors.affiliation: Biotechnology Division, Defence Research and Development Establishment, Gwalior 474002, India. Electronic address: syimteyaz@gmail.com.
authors.forename: Syed Imteyaz
authors.initials: SI
authors.lastname: Alam
authors.affiliation: Defence Research and Development Organisation, New Delhi, India.
authors.forename: Om
authors.initials: O
authors.lastname: Kumar
authors.affiliation: Biotechnology Division, Defence Research and Development Establishment, Gwalior 474002, India.
authors.forename: Ravi Bhushan
authors.initials: RB
authors.lastname: Kumar
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology; D003016:Clostridium perfringens / Q000235:genetics / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D005738:Gas Gangrene / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D008074:Lipoproteins / Q000276:immunology*; D008807:Mice, Inbred BALB C; D010802:Phylogeny; D011994:Recombinant Proteins / Q000276:immunology; D014611:Vaccination
chemical_list: D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D008074:Lipoproteins; D011994:Recombinant Proteins
keywords: Clostridium perfringens; Lipoprotein; Vaccine candidate; Virulence determinants