title: Dual-Enzyme-Loaded Multifunctional Hybrid Nanogel System for Pathological Responsive Ultrasound Imaging and T2-Weighted Magnetic Resonance Imaging.
abstract: A dual-enzyme-loaded multifunctional hybrid nanogel probe (SPIO@GCS/acryl/biotin-CAT/SOD-gel, or SGC) has been developed for dual-modality pathological responsive ultrasound (US) imaging and enhanced T2-weighted magnetic resonance (MR) imaging. This probe is composed of functionalized superparamagnetic iron oxide particles, a dual enzyme species (catalase and superoxide dismutase), and a polysaccharide cationic polymer glycol chitosan gel. The dual-modality US/MR imaging capabilities of the hybrid nanogel for responsive US imaging and enhanced T2-weighted MR imaging have been evaluated both in vitro and in vivo. These results show that the hybrid nanogel SGC can exhibit efficient dual-enzyme biocatalysis with pathological species for responsive US imaging. SGC also demonstrates increased accumulation in acidic environments for enhanced T2-weighted MR imaging. Further research on these nanogel systems may lead to the development of more efficient US/MR contrast agents.
authors.affiliation: †Department of Chemistry and Advanced Research Institute, Tongji University, Shanghai, 200092, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Wang
authors.affiliation: ‡Lab of Low-Dimensional Materials Chemistry, Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai, 200237, China.
authors.forename: Dechao
authors.initials: D
authors.lastname: Niu
authors.affiliation: §Department of Medical Ultrasound, Shanghai Tenth People's Hospital, Tenth People's Hospital of Tongji University, Shanghai, 200072, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Li
authors.affiliation: †Department of Chemistry and Advanced Research Institute, Tongji University, Shanghai, 200092, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wu
authors.affiliation: §Department of Medical Ultrasound, Shanghai Tenth People's Hospital, Tenth People's Hospital of Tongji University, Shanghai, 200072, China.
authors.forename: Xiaowan
authors.initials: X
authors.lastname: Bo
authors.affiliation: §Department of Medical Ultrasound, Shanghai Tenth People's Hospital, Tenth People's Hospital of Tongji University, Shanghai, 200072, China.
authors.forename: Boji
authors.initials: B
authors.lastname: Liu
authors.affiliation: †Department of Chemistry and Advanced Research Institute, Tongji University, Shanghai, 200092, China.
authors.forename: Song
authors.initials: S
authors.lastname: Bao
authors.affiliation: †Department of Chemistry and Advanced Research Institute, Tongji University, Shanghai, 200092, China.
authors.forename: Teng
authors.initials: T
authors.lastname: Su
authors.affiliation: §Department of Medical Ultrasound, Shanghai Tenth People's Hospital, Tenth People's Hospital of Tongji University, Shanghai, 200072, China.
authors.forename: Huixiong
authors.initials: H
authors.lastname: Xu
authors.affiliation: †Department of Chemistry and Advanced Research Institute, Tongji University, Shanghai, 200092, China.
authors.forename: Qigang
authors.initials: Q
authors.lastname: Wang
mesh_terms: D055162:Biocatalysis; D002374:Catalase / Q000378:metabolism*; D002470:Cell Survival; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000175:diagnosis*; D008279:Magnetic Resonance Imaging; D064251:Multifunctional Enzymes / Q000378:metabolism*; D000080385:Nanogels; D011092:Polyethylene Glycols / Q000737:chemistry / Q000378:metabolism*; D011094:Polyethyleneimine / Q000737:chemistry / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism*; D014465:Ultrasonics
chemical_list: D064251:Multifunctional Enzymes; D000080385:Nanogels; C413692:polyethylene glycol polyethyleneimine nanogel; D011092:Polyethylene Glycols; D011094:Polyethyleneimine; D002374:Catalase; D013482:Superoxide Dismutase
keywords: hybrid nanogels; magnetic resonance imaging; pathological responsiveness; ultrasound imaging