title: Novel MHC class I full-length allele and haplotype characterization in sooty mangabeys.
abstract: Sooty mangabeys (Cercocebus atys) are natural SIV hosts and the presumed source of HIV-2 and SIVmac, which makes them a valuable model for HIV/SIV research. However, like other African primates, little is known about their major histocompatibility complex (MHC) genetics. In this study, we used Roche/454 and Illumina MiSeq deep sequencing in order to determine the MHC class I transcripts in a cohort of 165 sooty mangabeys from the Yerkes National Primate Research Center (YNPRC). We have characterized 121 functionally full-length classical (Ceat-A and Ceat-B) and non-classical (Ceat-F and Ceat-I) alleles and have also identified 22 Ceat-A/Ceat-B haplotype chromosomal combinations. We correlated these Ceat-A/Ceat-B haplotype combinations to recently described microsatellite haplotypes from the YNPRC colony. These newly identified alleles and haplotypes establish a resource for studying cellular immunity in sooty mangabeys and provide a framework for rapidly cataloging MHC class I sequences in an understudied, yet important, nonhuman primate species.
authors.affiliation: Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, WI, 53715, USA.
authors.forename: Katelyn E
authors.initials: KE
authors.lastname: Heimbruch
authors.forename: Julie A
authors.initials: JA
authors.lastname: Karl
authors.forename: Roger W
authors.initials: RW
authors.lastname: Wiseman
authors.forename: Dawn M
authors.initials: DM
authors.lastname: Dudley
authors.forename: Zach
authors.initials: Z
authors.lastname: Johnson
authors.forename: Amitinder
authors.initials: A
authors.lastname: Kaur
authors.forename: David H
authors.initials: DH
authors.lastname: O'Connor
mesh_terms: D000483:Alleles; D000818:Animals; D016665:Cercocebus atys / Q000235:genetics*; D006239:Haplotypes / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D016133:Polymerase Chain Reaction
chemical_list: D015395:Histocompatibility Antigens Class I