title: Tolerance engineering in bacteria for the production of advanced biofuels and chemicals.
abstract: During microbial production of solvent-like compounds, such as advanced biofuels and bulk chemicals, accumulation of the final product can negatively impact the cultivation of the host microbe and limit the production levels. Consequently, improving solvent tolerance is becoming an essential aspect of engineering microbial production strains. Mechanisms ranging from chaperones to transcriptional factors have been used to obtain solvent-tolerant strains. However, alleviating growth inhibition does not invariably result in increased production. Transporters specifically have emerged as a powerful category of proteins that bestow tolerance and often improve production but are difficult targets for cellular expression. Here we review strain engineering, primarily as it pertains to bacterial solvent tolerance, and the benefits and challenges associated with the expression of membrane-localized transporters in improving solvent tolerance and production.
authors.affiliation: Joint BioEnergy Institute, 5885 Hollis St, Emeryville, CA 94608, USA; Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. Electronic address: amukhopadhyay@lbl.gov.
authors.forename: Aindrila
authors.initials: A
authors.lastname: Mukhopadhyay
mesh_terms: D001419:Bacteria / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D056804:Biofuels / Q000633:toxicity*; D004361:Drug Tolerance; D015870:Gene Expression; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D060847:Metabolic Engineering; D012997:Solvents / Q000633:toxicity*
chemical_list: D056804:Biofuels; D026901:Membrane Transport Proteins; D012997:Solvents
keywords: bacterial host engineering; biofuel production; efflux pump; solvent tolerance; transporters