title: Prescribed nanoparticle cluster architectures and low-dimensional arrays built using octahedral DNA origami frames.
abstract: Three-dimensional mesoscale clusters that are formed from nanoparticles spatially arranged in pre-determined positions can be thought of as mesoscale analogues of molecules. These nanoparticle architectures could offer tailored properties due to collective effects, but developing a general platform for fabricating such clusters is a significant challenge. Here, we report a strategy for assembling three-dimensional nanoparticle clusters that uses a molecular frame designed with encoded vertices for particle placement. The frame is a DNA origami octahedron and can be used to fabricate clusters with various symmetries and particle compositions. Cryo-electron microscopy is used to uncover the structure of the DNA frame and to reveal that the nanoparticles are spatially coordinated in the prescribed manner. We show that the DNA frame and one set of nanoparticles can be used to create nanoclusters with different chiroptical activities. We also show that the octahedra can serve as programmable interparticle linkers, allowing one- and two-dimensional arrays to be assembled with designed particle arrangements.
authors.affiliation: Center for Functional Nanomaterials, Brookhaven National Laboratory, Upton, New York 11973, USA.
authors.forename: Ye
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Biosciences Department, Brookhaven National Laboratory, Upton, New York 11973, USA.
authors.forename: Tong
authors.initials: T
authors.lastname: Wang
authors.affiliation: Center for Functional Nanomaterials, Brookhaven National Laboratory, Upton, New York 11973, USA.
authors.forename: Wenyan
authors.initials: W
authors.lastname: Liu
authors.affiliation: Center for Functional Nanomaterials, Brookhaven National Laboratory, Upton, New York 11973, USA.
authors.forename: Huolin L
authors.identifier: 000000026521868X
authors.initials: HL
authors.lastname: Xin
authors.affiliation: 1] Biosciences Department, Brookhaven National Laboratory, Upton, New York 11973, USA [2] Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, New York 11794-5213, USA.
authors.forename: Huilin
authors.initials: H
authors.lastname: Li
authors.affiliation: 1] Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA [2] Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA [3] Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts 02115, USA.
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Ke
authors.affiliation: 1] Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA [2] Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA [3] Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts 02115, USA.
authors.forename: William M
authors.initials: WM
authors.lastname: Shih
authors.affiliation: Center for Functional Nanomaterials, Brookhaven National Laboratory, Upton, New York 11973, USA.
authors.forename: Oleg
authors.initials: O
authors.lastname: Gang
mesh_terms: D003460:Crystallization / Q000379:methods*; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure*; D008422:Materials Testing; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure*; D009690:Nucleic Acid Conformation; D010316:Particle Size; D066330:Printing, Three-Dimensional
chemical_list: D004247:DNA