title: The diagnostic value of circulating microRNAs for middle-aged (40-60-year-old) coronary artery disease patients.
abstract: OBJECTIVE
  Circulating microRNAs have been recognized as promising biomarkers for various diseases. The present study aimed to explore the potential roles of circulating miR-149, miR-424 and miR-765 as non-invasive biomarkers for the diagnosis of coronary artery disease in middle-aged (40-60-year-old) patients.
  
  
  METHODS
  Sixty-five stable coronary artery disease patients (49-57 years old), 30 unstable coronary artery disease patients (49-58 years old), and 32 non-coronary artery disease patients (49--57 years old) who were matched for age, sex, smoking habits, hypertension and diabetes were enrolled in this study. Total RNA was isolated from plasma with TRIzol reagent. Circulating miRNA levels were measured by quantitative real-time polymerase chain reaction.
  
  
  RESULTS
  Circulating miR-149 levels were decreased 4.49-fold in stable coronary artery disease patients (1.18 ± 0.84) and 5.09-fold in unstable coronary artery disease patients (1.04 ± 0.65) compared with non-coronary artery disease patients (5.30 ± 2.57) (p<0.001). Circulating miR-424 levels were reduced 3.6-fold in stable coronary artery disease patients (1.18 ± 0.60) and 5-fold in unstable coronary artery disease patients (0.86 ± 0.54) compared with non-coronary artery disease patients (4.35 ± 2.20) (p<0.001). In contrast, circulating miR-765 levels were elevated 3.98-fold in stable coronary artery disease patients (6.09 ± 2.27) and 5.33-fold in unstable coronary artery disease patients (8.17 ± 2.77) compared with non-coronary artery disease patients (1.53 ± 0.99) (p<0.001). Receiver operating characteristic curve analysis revealed that the respective areas under the curve for circulating miR-149, miR-424 and miR-765 were 0.938, 0.919 and 0.968 in stable CAD patients and 0.951, 0.960 and 0.977 in unstable coronary artery disease patients compared with non-coronary artery disease patients.
  
  
  CONCLUSIONS
  Our results suggest that circulating miR-149, miR-424 and miR-765 might be novel, non-invasive biomarkers for the diagnosis of coronary artery disease in middle-aged patients. However, future prospective trials in large patient cohorts are necessary before reaching a solid conclusion.
authors.affiliation: Department of Cardiology, Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Ali Sheikh Md
authors.initials: AS
authors.lastname: Sayed
authors.affiliation: Department of Cardiology, Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Xia
authors.affiliation: Department of Cardiology, Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Cardiology, Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Deng
authors.affiliation: Department of Gynecology and Obstetrics, Xiangya 3rd Hospital, Central South University, Changsha, China.
authors.forename: Umme
authors.initials: U
authors.lastname: Salma
authors.affiliation: Department of Pharmacology, School of Pharmaceutical Sciences, Central South University, Changsha, China.
authors.forename: Tingbo
authors.initials: T
authors.lastname: Li
authors.affiliation: Department of Cardiology, Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Deng
authors.affiliation: Department of Cardiology, Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Dafeng
authors.initials: D
authors.lastname: Yang
authors.affiliation: Department of Cardiology, Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Haoyang
authors.affiliation: Department of Cardiology, Xiangya Hospital, Central South University, Changsha, China.
authors.forename: TianLun
authors.initials: T
authors.lastname: Yang
authors.affiliation: Department of Pharmacology, School of Pharmaceutical Sciences, Central South University, Changsha, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Peng
mesh_terms: D000367:Age Factors; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D003324:Coronary Artery Disease / Q000097:blood* / Q000175:diagnosis*; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D015415:Biomarkers; C551557:MIRN149 microRNA, human; C554214:MIRN424 microrna, human; C000600151:MIRN765 microRNA, human; D035683:MicroRNAs