title: Improvement of solid material for affinity resins by application of long PEG spacers to capture the whole target complex of FK506.
abstract: Solid materials for affinity resins bearing long PEG spacers between a functional group used for immobilization of a bio-active compound and the solid surface were synthesized to capture not only small target proteins but also large and/or complex target proteins. Solid materials with PEG1000 or PEG2000 as spacers, which bear a benzenesulfonamide derivative, exhibited excellent selectivity between the specific binding protein carbonic anhydrase type II (CAII) and non-specific ones. These materials also exhibited efficacy in capturing a particular target at a maximal amount. Affinity resins using solid materials with PEG1000 or PEG2000 spacers, bear a FK506 derivative, successfully captured the whole target complex of specific binding proteins at the silver staining level, while all previously known affinity resins with solid materials failed to achieve this objective. These novel affinity resins captured other specific binding proteins such as dynamin and neurocalcin δ as well.
authors.affiliation: Department of Pharmacy, Hyogo University of Health Sciences, 1-3-6 Minatojima, Chuo-ku, Kobe 650-8530, Japan; KOBE Chemical Genetics., Inc. 2-2-2 Minatojima-nakamachi, Chuo-ku, Kobe 650-8530, Japan.
authors.forename: Miyuki
authors.initials: M
authors.lastname: Mabuchi
authors.affiliation: Department of Pharmacy, Hyogo University of Health Sciences, 1-3-6 Minatojima, Chuo-ku, Kobe 650-8530, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Shimizu
authors.affiliation: Department of Pharmacy, Hyogo University of Health Sciences, 1-3-6 Minatojima, Chuo-ku, Kobe 650-8530, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ueda
authors.affiliation: Faculty of Pharmaceutical Sciences, Kinki University, 3-4-1 Kowakae, Higashi-Osaka 577-8502, Japan.
authors.forename: Kuniko
authors.initials: K
authors.lastname: Mitamura
authors.affiliation: Faculty of Pharmaceutical Sciences, Kinki University, 3-4-1 Kowakae, Higashi-Osaka 577-8502, Japan.
authors.forename: Shigeo
authors.initials: S
authors.lastname: Ikegawa
authors.affiliation: Department of Pharmacy, Hyogo University of Health Sciences, 1-3-6 Minatojima, Chuo-ku, Kobe 650-8530, Japan; KOBE Chemical Genetics., Inc. 2-2-2 Minatojima-nakamachi, Chuo-ku, Kobe 650-8530, Japan. Electronic address: tanaka-a@huhs.ac.jp.
authors.forename: Akito
authors.initials: A
authors.lastname: Tanaka
mesh_terms: D019703:Calcineurin / Q000737:chemistry / Q000378:metabolism; D024402:Carbonic Anhydrase II / Q000737:chemistry / Q000378:metabolism; D015195:Drug Design; D011092:Polyethylene Glycols / Q000737:chemistry*; D011485:Protein Binding; D012117:Resins, Synthetic / Q000737:chemistry*; D013449:Sulfonamides / Q000737:chemistry / Q000378:metabolism; D016559:Tacrolimus / Q000737:chemistry* / Q000378:metabolism; D000096926:Benzenesulfonamides
chemical_list: D019703:Calcineurin; D024402:Carbonic Anhydrase II; D011092:Polyethylene Glycols; D012117:Resins, Synthetic; D013449:Sulfonamides; D016559:Tacrolimus; D000096926:Benzenesulfonamides
keywords: Affinity resins; Benzenesulfonamide; Calcineurin; Carbonic anhydrase II; FK506; FKBP12; PEG; Target complex; Target identification