title: [Microbiological air quality in some kindergartens and antibiotic resistance of bacteria of the Staphylococcus spp. genus].
abstract: BACKGROUND
  Microbiological contamination or tne air and the acquisition of the antibiotic resistance by pathogenic bacteria is a growing phenomenon that has a substantial impact on the quality of our health. This problem applies mainly to public areas where we spend a large part of our lives. This study was focused on the microbiological analysis of the air in some kindergartens and antibiotic resistance of bacteria of the Stephylococcus spp. genus. The identification of the isolated mould fungi has been also made.
  
  
  METHODS
  Air samples were collected from classrooms in the seasonal cycle in the mornings and afternoons using 2 methods, sedimentation and impact. Air samples collected outside the kindergartens served as controls. Air quality assessments were based on the groups of indicator microorganisms, according to Polish standards. The susceptibility of isolated staphylococci was assessed with the disc-diffusion method, using 8 different classes of antibiotics, in line with the recommendations of the European Committee on Antimicrobial Susceptibility Testing (EUCAST).
  
  
  RESULTS
  The analyses show that, regardless of the method, the total number of heterothropic bacteria and staphylococci in the air of the analyzed kindergartens exceeded the allowable limits. There was no air-pollution with the fungal infection. Based on the antibiogram, it was found that Staphylococcus spp. strains showed the highest sensitivity to chloramphenicol and the lowest to penicillin and gentamicin. Among the fungi moulds of the genus Cladosporium predominated.
  
  
  CONCLUSIONS
  The results of the analyses highlight the need for regular health checks and further research to help identify biological factors that may significantly affect the quality of health of people living in public spaces.
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Kubera
authors.forename: Joanna
authors.initials: J
authors.lastname: Studzińska
authors.forename: Wioletta
authors.initials: W
authors.lastname: Dokładna
authors.forename: Marta
authors.initials: M
authors.lastname: Małecka-Adamowicz
authors.forename: Wojciech
authors.initials: W
authors.lastname: Donderski
mesh_terms: D000391:Air Microbiology; D016902:Air Pollution, Indoor / Q000032:analysis*; D002648:Child; D002669:Child Welfare / Q000706:statistics & numerical data*; D002675:Child, Preschool; D015169:Colony Count, Microbial; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects; D005260:Female; D006801:Humans; D019570:Inhalation Exposure / Q000032:analysis*; D008297:Male; D011044:Poland / Q000453:epidemiology; D012130:Respiratory Hypersensitivity / Q000517:prevention & control; D012578:Schools, Nursery; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000382:microbiology*