title: Novel glass slide preparation system for single DNA molecules analysis.
abstract: Here we propose an easy to build up and apply glass slide preparation system for single DNA molecules stretching. It is based on fast and simple coating of a solid glass with a cocktail of acrylic monomers that are easily polymerized via ultraviolet illumination. The acrylated slides are used to successfully stretch DNA molecules in a broader pH range compared to that of the commonly used amino-silanes. Moreover, the single DNA molecules that are stretched on the acrylated slides give a brighter and more photostable signal when visualized under a fluorescent microscope.
authors.affiliation: Institute of Molecular Biology "Roumen Tsanev", Bulgarian Academy of Sciences , Sofia , Bulgaria.
authors.forename: Alexander
authors.initials: A
authors.lastname: Zarkov
authors.affiliation: Institute of Molecular Biology "Roumen Tsanev", Bulgarian Academy of Sciences , Sofia , Bulgaria.
authors.forename: Stoyno
authors.initials: S
authors.lastname: Stoynov
authors.affiliation: Institute of Molecular Biology "Roumen Tsanev", Bulgarian Academy of Sciences , Sofia , Bulgaria.
authors.forename: Marina
authors.initials: M
authors.lastname: Nedelcheva-Veleva
keywords: DNA stretching; fluorescent microscopy; glass slide surface modification; single DNA molecules