title: A novel peptide-based recognition probe for the sensitive detection of CD44 on breast cancer stem cells.
abstract: Metastasis and recurrence of breast cancer remain significant clinical problems. The expression level of CD44 protein is higher in breast cancer-initiating cancer stem cells; therefore, the early detection of CD44 using a sensitive diagnostic probe is important for breast cancer diagnosis and therapeutic purposes. In this study, we fabricated a polyvalent directed peptide polymer (PDPP) that specifically recognized the CD44 biomarker, as confirmed by immunocytochemistry tests and fluorescence-activated cell sorting assessment. Our results indicate that PDPP is useful as a novel tool for the sensitive detection of breast cancer stem cells.
authors.affiliation: Department of Chemistry and Research Institute of Natural Sciences, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 133-791, Republic of Korea.
authors.forename: Jun-Haeng
authors.initials: JH
authors.lastname: Cho
authors.affiliation: Department of Chemistry and Research Institute of Natural Sciences, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 133-791, Republic of Korea.
authors.forename: Sang-Choon
authors.initials: SC
authors.lastname: Lee
authors.affiliation: Department of Chemistry and Research Institute of Natural Sciences, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 133-791, Republic of Korea.
authors.forename: Na-Reum
authors.initials: NR
authors.lastname: Ha
authors.affiliation: Department of Life Science, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 133-791, Republic of Korea.
authors.forename: Su-Jae
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Department of Chemistry and Research Institute of Natural Sciences, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 133-791, Republic of Korea. Electronic address: myyoon@hanyang.ac.kr.
authors.forename: Moon-Young
authors.initials: MY
authors.lastname: Yoon
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D045744:Cell Line, Tumor; D020650:Combinatorial Chemistry Techniques / Q000379:methods; D055088:Early Detection of Cancer; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D018960:Hyaluronan Receptors / Q000737:chemistry / Q000378:metabolism*; D061986:MCF-7 Cells; D051379:Mice; D025202:Molecular Diagnostic Techniques; D041681:NIH 3T3 Cells; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D012680:Sensitivity and Specificity; D013388:Succinimides / Q000737:chemistry
chemical_list: D018960:Hyaluronan Receptors; D010455:Peptides; D013388:Succinimides; C018151:N-succinimidyl 3-(2-pyridyldithio)propionate
keywords: Breast cancer stem cell; CD44; PDPP; Peptide