title: RiboTALE: A modular, inducible system for accurate gene expression control.
abstract: A limiting factor in synthetic gene circuit design is the number of independent control elements that can be combined together in a single system. Here, we present RiboTALEs, a new class of inducible repressors that combine the specificity of TALEs with the ability of riboswitches to recognize exogenous signals and differentially control protein abundance. We demonstrate the capacity of RiboTALEs, constructed through different combinations of TALE proteins and riboswitches, to rapidly and reproducibly control the expression of downstream targets with a dynamic range of 243.7 ± 17.6-fold, which is adequate for many biotechnological applications.
authors.affiliation: UC Davis Genome Center, University of California-Davis, Davis, CA, USA.
authors.forename: Navneet
authors.initials: N
authors.lastname: Rai
authors.affiliation: UC Davis Undergraduate Program, University of California-Davis, Davis, CA, USA.
authors.forename: Aura
authors.initials: A
authors.lastname: Ferreiro
authors.affiliation: UC Davis Undergraduate Program, University of California-Davis, Davis, CA, USA.
authors.forename: Alexander
authors.initials: A
authors.lastname: Neckelmann
authors.affiliation: UC Davis Undergraduate Program, University of California-Davis, Davis, CA, USA.
authors.forename: Amy
authors.initials: A
authors.lastname: Soon
authors.affiliation: 1] UC Davis Genome Center, University of California-Davis, Davis, CA, USA [2] Department of Biomedical Engineering, University of California-Davis, Davis, CA, USA.
authors.forename: Andrew
authors.initials: A
authors.lastname: Yao
authors.affiliation: 1] UC Davis Genome Center, University of California-Davis, Davis, CA, USA [2] Department of Biochemistry &Molecular Medicine, University of California-Davis, Davis, CA, USA [3] Department of Chemistry, University of California-Davis, Davis, CA, USA.
authors.forename: Justin
authors.initials: J
authors.lastname: Siegel
authors.affiliation: 1] UC Davis Genome Center, University of California-Davis, Davis, CA, USA [2] Department of Biomedical Engineering, University of California-Davis, Davis, CA, USA.
authors.forename: Marc T
authors.initials: MT
authors.lastname: Facciotti
authors.affiliation: 1] UC Davis Genome Center, University of California-Davis, Davis, CA, USA [2] Department of Computer Science, University of California-Davis, Davis, CA, USA.
authors.forename: Ilias
authors.initials: I
authors.lastname: Tagkopoulos
mesh_terms: D001709:Biotechnology / Q000295:instrumentation* / Q000379:methods; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D005813:Genes, Synthetic