title: Protective Efficacy of Recombinant Modified Vaccinia Virus Ankara Delivering Middle East Respiratory Syndrome Coronavirus Spike Glycoprotein.
abstract: Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe respiratory disease in humans. We tested a recombinant modified vaccinia virus Ankara (MVA) vaccine expressing full-length MERS-CoV spike (S) glycoprotein by immunizing BALB/c mice with either intramuscular or subcutaneous regimens. In all cases, MVA-MERS-S induced MERS-CoV-specific CD8(+) T cells and virus-neutralizing antibodies. Vaccinated mice were protected against MERS-CoV challenge infection after transduction with the human dipeptidyl peptidase 4 receptor. This MERS-CoV infection model demonstrates the safety and efficacy of the candidate vaccine.
authors.affiliation: German Center for Infection Research (DZIF), Institute for Infectious Diseases and Zoonoses, LMU University of Munich, Munich, Germany.
authors.forename: Asisa
authors.initials: A
authors.lastname: Volz
authors.affiliation: German Center for Infection Research (DZIF), Institute of Virology, Philipps University Marburg, Marburg, Germany.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Kupke
authors.affiliation: German Center for Infection Research (DZIF), Institute for Infectious Diseases and Zoonoses, LMU University of Munich, Munich, Germany.
authors.forename: Fei
authors.initials: F
authors.lastname: Song
authors.affiliation: German Center for Infection Research (DZIF), Institute for Infectious Diseases and Zoonoses, LMU University of Munich, Munich, Germany.
authors.forename: Sylvia
authors.initials: S
authors.lastname: Jany
authors.affiliation: German Center for Infection Research (DZIF), Institute for Infectious Diseases and Zoonoses, LMU University of Munich, Munich, Germany.
authors.forename: Robert
authors.initials: R
authors.lastname: Fux
authors.affiliation: German Center for Infection Research (DZIF), Institute of Virology, Philipps University Marburg, Marburg, Germany.
authors.forename: Hosam
authors.initials: H
authors.lastname: Shams-Eldin
authors.affiliation: German Center for Infection Research (DZIF), Institute of Virology, Philipps University Marburg, Marburg, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Schmidt
authors.affiliation: University of Giessen Lung Center, Department of Internal Medicine II, Section of Infectious Diseases, Giessen, Germany.
authors.forename: Christin
authors.initials: C
authors.lastname: Becker
authors.affiliation: German Center for Infection Research (DZIF), Institute of Virology, Philipps University Marburg, Marburg, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Eickmann
authors.affiliation: German Center for Infection Research (DZIF), Institute of Virology, Philipps University Marburg, Marburg, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Becker
authors.affiliation: German Center for Infection Research (DZIF), Institute for Infectious Diseases and Zoonoses, LMU University of Munich, Munich, Germany gerd.sutter@lmu.de.
authors.forename: Gerd
authors.initials: G
authors.lastname: Sutter
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D018352:Coronavirus Infections / Q000517:prevention & control*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C; D065207:Middle East Respiratory Syndrome Coronavirus / Q000276:immunology*; D064370:Spike Glycoprotein, Coronavirus / Q000276:immunology / Q000378:metabolism*; D014616:Vaccinia virus / Q000235:genetics*; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D064370:Spike Glycoprotein, Coronavirus; D014765:Viral Vaccines