title: Light scattering as an intrinsic indicator for pancreatic islet cell mass and secretion.
abstract: The pancreatic islet of Langerhans is composed of endocrine cells producing and releasing hormones from secretory granules in response to various stimuli for maintenance of blood glucose homeostasis. In order to adapt to a variation in functional demands, these islets are capable of modulating their hormone secretion by increasing the number of endocrine cells as well as the functional response of individual cells. A failure in adaptive mechanisms will lead to inadequate blood glucose regulation and thereby to the development of diabetes. It is therefore necessary to develop tools for the assessment of both pancreatic islet mass and function, with the aim of understanding cellular regulatory mechanisms and factors guiding islet plasticity. Although most of the existing techniques rely on the use of artificial indicators, we present an imaging methodology based on intrinsic optical properties originating from mature insulin secretory granules within endocrine cells that reveals both pancreatic islet mass and function. We demonstrate the advantage of using this imaging strategy by monitoring in vivo scattering signal from pancreatic islets engrafted into the anterior chamber of the mouse eye, and how this versatile and noninvasive methodology permits the characterization of islet morphology and plasticity as well as hormone secretory status.
authors.affiliation: The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, Karolinska University Hospital L1, Stockholm SE-171 76, Sweden.
authors.forename: E
authors.initials: E
authors.lastname: Ilegems
authors.affiliation: The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, Karolinska University Hospital L1, Stockholm SE-171 76, Sweden.
authors.forename: P P
authors.initials: PP
authors.lastname: van Krieken
authors.affiliation: The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, Karolinska University Hospital L1, Stockholm SE-171 76, Sweden.
authors.forename: P K
authors.initials: PK
authors.lastname: Edlund
authors.affiliation: The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, Karolinska University Hospital L1, Stockholm SE-171 76, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Dicker
authors.affiliation: The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, Karolinska University Hospital L1, Stockholm SE-171 76, Sweden.
authors.forename: T
authors.initials: T
authors.lastname: Alanentalo
authors.affiliation: Umeå Centre for Molecular Medicine, Umeå University, Umeå, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Eriksson
authors.affiliation: The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, Karolinska University Hospital L1, Stockholm SE-171 76, Sweden.
authors.forename: S
authors.initials: S
authors.lastname: Mandic
authors.affiliation: Umeå Centre for Molecular Medicine, Umeå University, Umeå, Sweden.
authors.forename: U
authors.initials: U
authors.lastname: Ahlgren
authors.affiliation: 1] The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, Karolinska University Hospital L1, Stockholm SE-171 76, Sweden [2] Diabetes Research Institute, Miller School of Medicine, University of Miami, FL 33136, Miami [3] Lee Kong Chian School of Medicine, Nanyang Technological University, Imperial College London, Novena Campus, Singapore.
authors.forename: P-O
authors.initials: PO
authors.lastname: Berggren
mesh_terms: D000818:Animals; D000067493:Dynamic Light Scattering; D007515:Islets of Langerhans / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D051379:Mice; D009929:Organ Size; D010187:Pancreatic Hormones / Q000378:metabolism; D051381:Rats
chemical_list: D010187:Pancreatic Hormones