title: [The dynamics of indicators of selenium, glutathione and anti-oxidant defense of blood in patients with anemic cardiomyopathy against the background of treatment with preparations of iron and selenium].
abstract: The study was carried out on samplings of 46 patients with asiderotic anemia of severe degree and complicated by cardiomyopathy and 16 healthy persons. The content of selenium was analyzed using I.I. Nazarenko technique of detection of mass concentration GOST 19413-89. The content of glutathione in blood was detected using the technique based on capacity of acid-soluble thiol aggregations at interaction with 5,5-dithio-bis(2-nitrobenzene) acid to form a colored compound--thio-2-nitrobenzene acid. The principle of technique of measurement of activity of glutathione peroxidase of blood ervthrocvtes is in capacity of peroxide hydrogen to form a resistant colored complex with molybdenum salts. The technique of detection of activity of glutathione peroxidase is based on its capacity to catalyze reaction of interaction of reduced glutathione with tretbutyl hydro peroxide and on capacity of glutathione reductase to catalyze NADFN-dependent reduction of oxidated glutathione. The principle of technique of detection of activity of superoxiddismutase is based on capacity of enzyme to suppress reaction of reduction of nitro blue tetrazolium with superoxide anion-radical generated in vitro in the system xanthine-xanthineoxidase. The study established decreasing of content of selenium in blood of patients with anemic cardiomyopathy up to 1.8 times as compared with control group. The content of total glutathione in blood of patients was decreased up to 17.7% at the expense of decreasing of level of reduced glutathione up to 18.5%. The study established decreasing of activity of catalase in erythrocytes up to 1.3 times, glutathione peroxidase up to 2.5 times, glutathione reductase up to 2.1 times and superoxiddismutase up to 1.5 times as compared with control group. After the preparations of iron and selenium ware applied to patients with anemic cardiomyopathy the increase of level of selenium in blood up to 80.4% was established. The level of total glutathione increased up to 54.5% at the expense of increase of content of reduced glutathione up to 59.5%. The activity of blood erythrocytes in patients against the background of treatment increased up to 9.1%, the activity of glutathione peroxidase, glutathione reductase and superoxiddismutase of blood erythrocytes increased up to 3, 2.1 and 2 times respectively.
authors.forename: E V
authors.initials: EV
authors.lastname: Goncharova
authors.forename: A V
authors.initials: AV
authors.lastname: Govorin
authors.forename: O A
authors.initials: OA
authors.lastname: Scherbakova
authors.forename: M V
authors.initials: MV
authors.lastname: Chistiakova
mesh_terms: D000328:Adult; D000740:Anemia / Q000097:blood* / Q000473:pathology; D000975:Antioxidants / Q000302:isolation & purification*; D009202:Cardiomyopathies / Q000097:blood* / Q000473:pathology; D002374:Catalase / Q000097:blood; D005260:Female; D005978:Glutathione / Q000097:blood; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D007501:Iron / Q000097:blood; D008297:Male; D008875:Middle Aged; D012643:Selenium / Q000097:blood*; D013482:Superoxide Dismutase
chemical_list: D000975:Antioxidants; D007501:Iron; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D012643:Selenium