title: Dynamic Changes in ANGUSTIFOLIA3 Complex Composition Reveal a Growth Regulatory Mechanism in the Maize Leaf.
abstract: Most molecular processes during plant development occur with a particular spatio-temporal specificity. Thus far, it has remained technically challenging to capture dynamic protein-protein interactions within a growing organ, where the interplay between cell division and cell expansion is instrumental. Here, we combined high-resolution sampling of the growing maize (Zea mays) leaf with tandem affinity purification followed by mass spectrometry. Our results indicate that the growth-regulating SWI/SNF chromatin remodeling complex associated with ANGUSTIFOLIA3 (AN3) was conserved within growing organs and between dicots and monocots. Moreover, we were able to demonstrate the dynamics of the AN3-interacting proteins within the growing leaf, since copurified GROWTH-REGULATING FACTORs (GRFs) varied throughout the growing leaf. Indeed, GRF1, GRF6, GRF7, GRF12, GRF15, and GRF17 were significantly enriched in the division zone of the growing leaf, while GRF4 and GRF10 levels were comparable between division zone and expansion zone in the growing leaf. These dynamics were also reflected at the mRNA and protein levels, indicating tight developmental regulation of the AN3-associated chromatin remodeling complex. In addition, the phenotypes of maize plants overexpressing miRNA396a-resistant GRF1 support a model proposing that distinct associations of the chromatin remodeling complex with specific GRFs tightly regulate the transition between cell division and cell expansion. Together, our data demonstrate that advancing from static to dynamic protein-protein interaction analysis in a growing organ adds insights in how developmental switches are regulated.
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors.forename: Hilde
authors.identifier: http://orcid.org/0000-0001-7494-1290
authors.initials: H
authors.lastname: Nelissen
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors.forename: Dominique
authors.identifier: http://orcid.org/0000-0001-5770-7670
authors.initials: D
authors.lastname: Eeckhout
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors.forename: Kirin
authors.initials: K
authors.lastname: Demuynck
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors.forename: Geert
authors.initials: G
authors.lastname: Persiau
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium Department of Medical Protein Research, VIB, 9000 Ghent, Belgium Department of Biochemistry, Ghent University, 9000 Ghent, Belgium.
authors.forename: Alan
authors.initials: A
authors.lastname: Walton
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors.forename: Michiel
authors.initials: M
authors.lastname: van Bel
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors.forename: Marieke
authors.initials: M
authors.lastname: Vervoort
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors.forename: Jasper
authors.initials: J
authors.lastname: Candaele
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors.forename: Jolien
authors.identifier: http://orcid.org/0000-0003-0047-4380
authors.initials: J
authors.lastname: De Block
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors.forename: Stijn
authors.initials: S
authors.lastname: Aesaert
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors.forename: Mieke
authors.identifier: http://orcid.org/0000-0002-7632-1463
authors.initials: M
authors.lastname: Van Lijsebettens
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors.forename: Sofie
authors.identifier: http://orcid.org/0000-0001-6195-9889
authors.initials: S
authors.lastname: Goormachtig
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors.forename: Klaas
authors.identifier: http://orcid.org/0000-0003-4790-2725
authors.initials: K
authors.lastname: Vandepoele
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors.forename: Jelle
authors.identifier: http://orcid.org/0000-0002-4932-8192
authors.initials: J
authors.lastname: Van Leene
authors.affiliation: Department of Genetics, Development, and Cell Biology, Iowa State University, Iowa 50011-3268.
authors.forename: Michael
authors.initials: M
authors.lastname: Muszynski
authors.affiliation: Department of Medical Protein Research, VIB, 9000 Ghent, Belgium Department of Biochemistry, Ghent University, 9000 Ghent, Belgium.
authors.forename: Kris
authors.identifier: http://orcid.org/0000-0002-4237-0283
authors.initials: K
authors.lastname: Gevaert
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors.forename: Dirk
authors.identifier: http://orcid.org/0000-0002-3217-8407
authors.initials: D
authors.lastname: Inzé
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium geert.dejaeger@psb.vib-ugent.be.
authors.forename: Geert
authors.initials: G
authors.lastname: De Jaeger
mesh_terms: D017124:Conserved Sequence / Q000235:genetics / Q000502:physiology; D010937:Plant Growth Regulators / Q000235:genetics / Q000502:physiology; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development*; D010940:Plant Proteins / Q000235:genetics / Q000502:physiology*; D053719:Tandem Mass Spectrometry; D003313:Zea mays / Q000235:genetics*
chemical_list: D010937:Plant Growth Regulators; D010940:Plant Proteins