title: Design of enzyme-interfaced DNA logic operations (AND, OR and INHIBIT) with an assaying application for single-base mismatch.
abstract: We devised AND, OR and INHIBIT logic gates. They were based on the enzyme-induced DNA base flipping mechanism, which caused significant local conformation changes in DNA. This can be monitored via photonic signals as outputs. We also provided a prototype of a built-in biosensor capable of distinguishing single-base mismatches in a DNA duplex.
authors.affiliation: Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, School of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China. malong@tust.edu.cn.
authors.forename: Long
authors.initials: L
authors.lastname: Ma
authors.forename: Aipo
authors.initials: A
authors.lastname: Diao
mesh_terms: D020137:Base Pair Mismatch; D039301:Computers, Molecular; D004247:DNA / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D008128:Logic; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D011487:Protein Conformation; D013050:Spectrometry, Fluorescence
chemical_list: D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific; C037130:GCGC-specific type II deoxyribonucleases