title: Cuprizone Model as a Tool for Preclinical Studies of the Efficacy of Multiple Sclerosis Diagnosis and Therapy.
abstract: To study demyelination and remyelination processes and their response to different drugs, a protocol for modeling multiple sclerosis using the copper chelator cuprizone was developed. Magnetic resonance imaging confirmed the presence of demyelination lesions on week 4 of 0.6% cuprizone-containing diet. Immunohistochemical staining with polyclonal antibodies to glial fibrillary acidic protein (pAb GFAP) confirmed the increase in the number of reactive astrocytes on week 4 of diet and during remyelination (week 2 after diet). Analysis of neurophysiological functions in mice with cuprizone-induced demyelination revealed motor and behavioral deficits. This model can be used as a tool for preclinical studies of the efficiency of multiple sclerosis diagnostic and therapy.
authors.affiliation: V. P. Serbsky State Research Center for Social and Forensic Psychiatry, Ministry of Health of the Russian Federation, Moscow, Russia, abakumova@serbsky.ru.
authors.forename: T O
authors.initials: TO
authors.lastname: Abakumova
authors.forename: A A
authors.initials: AA
authors.lastname: Kuz'kina
authors.forename: M E
authors.initials: ME
authors.lastname: Zharova
authors.forename: M V
authors.initials: MV
authors.lastname: Zharova
authors.forename: D A
authors.initials: DA
authors.lastname: Pozdeeva
authors.forename: I L
authors.initials: IL
authors.lastname: Gubskii
authors.forename: I I
authors.initials: II
authors.lastname: Shepeleva
authors.forename: O M
authors.initials: OM
authors.lastname: Antonova
authors.forename: N V
authors.initials: NV
authors.lastname: Nukolova
authors.forename: Z I
authors.initials: ZI
authors.lastname: Kekelidze
authors.forename: V P
authors.initials: VP
authors.lastname: Chekhonin
mesh_terms: D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging; D003471:Cuprizone / Q000494:pharmacology*; D003711:Demyelinating Diseases / Q000139:chemically induced*; D004195:Disease Models, Animal; D005260:Female; D005904:Glial Fibrillary Acidic Protein; D008279:Magnetic Resonance Imaging; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009103:Multiple Sclerosis / Q000139:chemically induced* / Q000628:therapy; D009186:Myelin Sheath / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D011859:Radiography
chemical_list: D005904:Glial Fibrillary Acidic Protein; D009419:Nerve Tissue Proteins; C528900:glial fibrillary astrocytic protein, mouse; D003471:Cuprizone