title: Folic-Acid-Targeted Self-Assembling Supramolecular Carrier for Gene Delivery.
abstract: A targeting gene carrier for cancer-specific delivery was successfully developed through a "multilayer bricks-mortar" strategy. The gene carrier was composed of adamantane-functionalized folic acid (FA-AD), an adamantane-functionalized poly(ethylene glycol) derivative (PEG-AD), and β-cyclodextrin-grafted low-molecular-weight branched polyethylenimine (PEI-CD). Carriers produced by two different self-assembly schemes, involving either precomplexation of the PEI-CD with the FA-AD and PEG-AD before pDNA condensation (Method A) or pDNA condensation with the PEI-CD prior to addition of the FA-AD and PEG-AD to engage host-guest complexation (Method B) were investigated for their ability to compact pDNA into nanoparticles. Cell viability studies show that the material produced by the Method A assembly scheme has lower cytotoxicity than branched PEI 25 kDa (PEI-25KD) and that the transfection efficiency is maintained. These findings suggest that the gene carrier, based on multivalent host-guest interactions, could be an effective, targeted, and low-toxicity carrier for delivering nucleic acid to target cells.
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500 (P. R. China).
authors.forename: Rongqiang
authors.initials: R
authors.lastname: Liao
authors.affiliation: Department of Oncology, Chongqing Cancer Hospital, Chongqing 400011 (P. R. China).
authors.forename: Shouhui
authors.initials: S
authors.lastname: Yi
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500 (P. R. China).
authors.forename: Manshuo
authors.initials: M
authors.lastname: Liu
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500 (P. R. China).
authors.forename: Wenling
authors.initials: W
authors.lastname: Jin
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500 (P. R. China). liaorongqiang@126.com, yangbo_kmust@126.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
mesh_terms: D000218:Adamantane / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004337:Drug Carriers / Q000737:chemistry* / Q000633:toxicity; D005492:Folic Acid / Q000737:chemistry* / Q000633:toxicity; D006801:Humans; D007624:KB Cells; D008970:Molecular Weight; D011092:Polyethylene Glycols / Q000737:chemistry; D011094:Polyethyleneimine / Q000737:chemistry; D014162:Transfection; D047392:beta-Cyclodextrins / Q000737:chemistry
chemical_list: D004337:Drug Carriers; D047392:beta-Cyclodextrins; D011092:Polyethylene Glycols; D011094:Polyethyleneimine; D004247:DNA; D005492:Folic Acid; D000218:Adamantane
keywords: cyclodextrins; drug delivery; gene carriers; polyethylenimine; self-assembly