title: Fibrogenesis and epithelial coating of skin wounds in rats treated with angico extract (Anadenanthera colubrina var. cebil).
abstract: OBJECTIVE
  To evaluate the effects of angico bark extract (Anadenanthera colubrina var. cebil) in the healing process of the skin of rats.
  
  
  METHODS
  Twenty adult male rats were divided into four groups of five animals each, according to the respective postoperative days, as follow: G4, G7, G14 and G21. Each group received two incisions on skin and subcutaneous tissue in the right and left antimere of the thoracic region, separated by a distance of 2 cm. The right lesion was treated daily with saline and the left with the angico alcoholic extract (5%). At the end of each experimental period, the animals were euthanized and fragments of the wound area with the edges were removed, fixed in 10% formaldehyde solution and processed for paraffin embedding. Histological sections (5 μm of thickness) were stained with hematoxylin and eosin (HE), Gomori trichromic and picrosisirus red for morphological and morphometric analyses. Statistical analysis was done by ANOVA and Tukey-Kramer test (p<0.05).
  
  
  RESULTS
  Morphological analysis showed larger fibroblasts and a higher concentration of collagen fibers in skyn wounds treated with the angico extract. Morphometric analysis demonstrated a significant increase in the number of fibroblasts at 7th and collagen in 7th and 14th days (p<0.01) in wounds treated with the angico extract.
  
  
  CONCLUSIONS
  The angico alcoholic extract (Anadenanthera colubrina var. cebil) induces the acceleration of wound healing in skin wounds of rats.
authors.affiliation: Department of Morphology, Federal University of Piaui, Teresina, PI, Brazil.
authors.forename: Wagner Soares
authors.initials: WS
authors.lastname: Pessoa
authors.affiliation: Department of Morphology and Animal Physiology, Federal Rural University of Pernambuco, Recife, PE, Brazil.
authors.forename: Lígia Reis de Moura
authors.initials: LR
authors.lastname: Estevão
authors.affiliation: Department of Morphology and Genetic, Federal University of Sao Paulo, Brazil.
authors.forename: Ricardo Santos
authors.initials: RS
authors.lastname: Simões
authors.affiliation: Department of Morphology, UFRPE, Recife, PE, Brazil.
authors.forename: Fábio de Souza
authors.initials: Fde S
authors.lastname: Mendonça
authors.affiliation: Department of Biology, UFPI, Picos, PI, Brazil.
authors.forename: Luis
authors.initials: L
authors.lastname: Evêncio-Luz
authors.affiliation: Department of Histology and Embryology, Federal University of Pernambuco, Brazil.
authors.forename: Liriane
authors.initials: L
authors.lastname: Baratella-Evêncio
authors.affiliation: Department of Morphology and Genetics, UNIFESP, Sao Paulo, SP, Brazil.
authors.forename: Rinaldo
authors.initials: R
authors.lastname: Florencio-Silva
authors.affiliation: Department of Morphology and Physiology, UFRPE, Recife, PE, Brazil.
authors.forename: Fabrício Bezerra de
authors.initials: FB
authors.lastname: Sá
authors.affiliation: Department of Morphology and Physiology, UFRPE, Recife, PE, Brazil.
authors.forename: Joaquim
authors.initials: J
authors.lastname: Evêncio-Neto
mesh_terms: D000818:Animals; D002452:Cell Count; D003094:Collagen / Q000032:analysis; D007887:Fabaceae / Q000737:chemistry*; D005347:Fibroblasts / Q000187:drug effects; D008297:Male; D016612:Paraffin Embedding; D008517:Phytotherapy / Q000379:methods*; D010936:Plant Extracts / Q000627:therapeutic use*; D011184:Postoperative Period; D051381:Rats; D015203:Reproducibility of Results; D012867:Skin / Q000187:drug effects* / Q000293:injuries / Q000473:pathology*; D040521:Subcutaneous Tissue / Q000187:drug effects / Q000293:injuries / Q000473:pathology; D013997:Time Factors; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D010936:Plant Extracts; D003094:Collagen