title: [Effect of budesonide on the expression of IL-12 in animal model of minimal persistent inflammation of allergic rhinitis in rats].
abstract: OBJECTIVE
  To investigate the influence of budesonide on animal model of minimal persistent inflammation (MPI) of allergic rhinitis in rats and to investigate the changes of interleukin-12 (IL-12) in nasal mucosa.
  
  
  METHODS
  Sixty Sprague-Dawley (SD) rats were randomly divided into four groups: group A (allergic rhinitis group), B (experimental group), C (MPI model group) and D (bland group) respectively, with fifteen animals in each group. Rats from group A,B and C were sensitized intraperitoneally by injection of suspension of ovalbumin (OVA) and aluminum hydroxide in 0.9% physiological saline. Then, repeated local booster sensitization with different concentration of OVA suspension (1% and 0.01%) or physiological saline into the nasal cavity of those rats were performed. For group D, physiological saline was used only. From 36th day, group B were given budesonide treatment for three weeks. A, C and D group were given normal saline nasal spray. Symptoms (sneezing) of rats after antigen challenge were observed and the infiltration of eosinophils (EOS) together with the expression of intercellular adhesion molecule 1 (ICAM-1) and IL-12 in the nasal epithelial cells were also examined.
  
  
  RESULTS
  When challenged with 1% OVA, the sneezing number of rats in group B was increased markedly than that in group D (P < 0.05). However, there was no difference between group B, A and C (P > 0.05). When challenged with 0.01% OVA and given budesonide, the symptom of sneezing almost disappeared in group B just like that in group D and there was no difference between the two groups (P > 0.05). Besides, there was still more EOS infiltrated in the nasal mucosa of rats in group C than that in group D (P < 0.05). There was no expression of ICAM-1 in nasal epithelium of rats in group D, nevertheless, ICAM-1 was found mildly expressed in group C. IL-12 expression was significantly increased compared with group A and group C, and was no significantly difference compared with bland group (P > 0.05).
  
  
  CONCLUSIONS
  Budesonide significantly inhibited the late reaction of animal model of minimal persistent inflammation (MPI) of allergic rhinitis in rats and increase the expression of IL-12 in MPI model.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Li
authors.forename: Manying
authors.initials: M
authors.lastname: Geng
mesh_terms: D000485:Allergens; D000818:Animals; D019819:Budesonide / Q000494:pharmacology*; D004195:Disease Models, Animal; D004804:Eosinophils / Q000276:immunology; D007249:Inflammation / Q000188:drug therapy*; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D018664:Interleukin-12 / Q000378:metabolism*; D007958:Leukocyte Count; D009297:Nasal Mucosa / Q000378:metabolism; D010047:Ovalbumin; D051381:Rats; D017207:Rats, Sprague-Dawley; D065631:Rhinitis, Allergic / Q000188:drug therapy*
chemical_list: D000485:Allergens; D018799:Intercellular Adhesion Molecule-1; D018664:Interleukin-12; D019819:Budesonide; D010047:Ovalbumin