title: Inhibitors of thermally induced burn incidents – characterization by microbiological procedure, electrophoresis, SEM, DSC and IR spectroscopy.
abstract: Differential scanning calorimetry (DSC) and thermogravimetric (TGA) investigations, acetate electrophoresis (CAE), Fourier-transform infrared spectrometry (FTIR), scanning electron microscopy (SEM) analysis and microbiological procedures were all carried out after heating the samples to a temperature sufficient for simulating a burn incident. In particular, the purpose of the present study was to analyze the effect of antioxidants, such as fucoidan from brown seaweed and flame-retardant cyclic organophosphates and phosphonates, on an organic chicken skin that gets changed by a burn incident. DSC was considered to be a useful tool in assessing in vitro temperature-mediated cross-linking; an innovative analytical conclusion was obtained from the experimentation described in the paper. FTIR tests revealed that heating a dry organic chicken skin to the boiling point leads to the disappearance of a wide band in the 1650-1550 cm(-1) area or the conversion of a band, which may be attributed to the intermolecular β-sheet aggregates. Fucoidan from brown seaweed and flame-retardant cyclic organophosphates and phosphonates probably bind with the collagen that is changed by the burn (in addition to the influence of antioxidant solutions on samples of a blank or not boiled organic chicken skin) incident forming a polymer film with the collagen of the chicken skin surface (SEM analysis), decreasing the aggregation process and native collagen recovery. Good bacteriostatic properties were determined for fucoidan samples from brown seaweed and flame-retardant cyclic organophosphates and phosphonates against the pathogenic bacteria Escherichia coli and Staphylococcus aureus. Thus, it was observed that the fucoidan incorporated into collagen films can be used as a therapeutically active biomaterial that speeds up the wound-healing process.
authors.affiliation: University of Bielsko-Biała, Faculty of Materials, Civil and Environmental Engineering, Bielsko-Biała, Poland. apielesz@ath.bielsko.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Pielesz
authors.forename: Alicja
authors.initials: A
authors.lastname: Machnicka
authors.forename: Andrzej
authors.initials: A
authors.lastname: Gawłowski
authors.forename: Janusz
authors.initials: J
authors.lastname: Fabia
authors.forename: Ewa
authors.initials: E
authors.lastname: Sarna
authors.forename: Włodzimierz
authors.initials: W
authors.lastname: Biniaś
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D002056:Burns / Q000188:drug therapy* / Q000209:etiology / Q000382:microbiology*; D002152:Calorimetry, Differential Scanning; D002645:Chickens; D004586:Electrophoresis; D004926:Escherichia coli / Q000187:drug effects / Q000502:physiology; D006358:Hot Temperature / Q000009:adverse effects*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D011134:Polysaccharides / Q000494:pharmacology / Q000627:therapeutic use; D012867:Skin / Q000187:drug effects / Q000382:microbiology; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects / Q000502:physiology
chemical_list: D000975:Antioxidants; D011134:Polysaccharides; C007789:fucoidan