title: [Frontiers in Live Bone Imaging Researches. In vivo imaging of bone marrow microenvironment].
abstract: The function of hematopoietic stem cells and leukemia stem cells depends on their interaction with complex microenvironment within the bone marrow. Conventional methods could not observe the dynamic cell movement in living bone marrow. Recently rapid development of live imaging techniques enables us to understand the cellular interaction. Intravital two-photon imaging is the ideal method to understand the nature of bone marrow because of visualizing the cellular dynamics in vivo and observing the bone marrow long time. Here we show the latest reports about bone marrow microenvironment by intravital imaging, and also discuss its further application.
authors.affiliation: Department of Immunology and Cell Biology, Graduate School of Medicine and Frontier Biosciences, Osaka University, Japan./Japan Science and Technology Agency, CREST, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Mizuno
authors.forename: Masaru
authors.initials: M
authors.lastname: Ishii
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure*; D002450:Cell Communication / Q000502:physiology; D060833:Cellular Microenvironment / Q000502:physiology*; D006412:Hematopoietic Stem Cells; D007938:Leukemia / Q000473:pathology; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton; D057054:Molecular Imaging / Q000379:methods* / Q000639:trends