title: The effect of manganese-induced toxicity on the cytokine mRNA expression of chicken spleen lymphocytes in vitro.
abstract: Manganese (Mn) is essential for life, but excess Mn exposure is harmful. This study investigated the effect of excess Mn on the cytokines of spleen lymphocytes in chicken. Lymphocytes were incubated with or without MnCl2 (2, 4, 6, and 8×10(-4) mmol/L) for 12, 24, 36, and 48 h, respectively. The mRNA expression of interleukin (IL) -2, IL-4, IL-6, IL-12β, and IL-17 and interferon (INF) -γ was examined using RT-PCR. Excess Mn inhibited IL-2, IL-4, IL-6, IL-12β, and IL-17 mRNA expression in chicken spleen lymphocytes in a dose-dependent manner. IFN-γ was inhibited by 8×10(-4) mmol/L Mn for 48 h. This study demonstrates that excess Mn affects cytokine mRNA expression and causes immunosuppression in chicken spleen lymphocytes. The relationships between IL-6 and IL-17 and between IL-2 and IL-12β were strong under immunosuppression caused by excess Mn in lymphocytes.
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Xinxing
authors.initials: X
authors.lastname: Lu
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Yihao
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Rensheng
authors.initials: R
authors.lastname: Bai
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China. Electronic address: lishu@neau.edu.cn.
authors.forename: Shu
authors.initials: S
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, PR China. Electronic address: tengxiaohua@neau.edu.cn.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Teng
mesh_terms: D000818:Animals; D002645:Chickens / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D007165:Immunosuppression Therapy / Q000662:veterinary*; D007371:Interferon-gamma / Q000378:metabolism; D053762:Interleukin-12 Subunit p40 / Q000378:metabolism; D020381:Interleukin-17 / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism*; D008345:Manganese / Q000633:toxicity*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D013154:Spleen / Q000166:cytology* / Q000187:drug effects; D013997:Time Factors
chemical_list: D016207:Cytokines; D053762:Interleukin-12 Subunit p40; D020381:Interleukin-17; D007376:Interleukin-2; D015850:Interleukin-6; D012333:RNA, Messenger; D015847:Interleukin-4; D008345:Manganese; D007371:Interferon-gamma
keywords: Chicken; Cytokine; Lymphocyte; Manganese; Spleen