title: Direct identification of on-bead peptides using surface-enhanced Raman spectroscopic barcoding system for high-throughput bioanalysis.
abstract: Recently, preparation and screening of compound libraries remain one of the most challenging tasks in drug discovery, biomarker detection, and biomolecular profiling processes. So far, several distinct encoding/decoding methods such as chemical encoding, graphical encoding, and optical encoding have been reported to identify those libraries. In this paper, a simple and efficient surface-enhanced Raman spectroscopic (SERS) barcoding method using highly sensitive SERS nanoparticles (SERS ID) is presented. The 44 kinds of SERS IDs were able to generate simple codes and could possibly generate more than one million kinds of codes by incorporating combinations of different SERS IDs. The barcoding method exhibited high stability and reliability under bioassay conditions. The SERS ID encoding based screening platform can identify the peptide ligand on the bead and also quantify its binding affinity for specific protein. We believe that our SERS barcoding technology is a promising method in the screening of one-bead-one-compound (OBOC) libraries for drug discovery.
authors.affiliation: Interdisciplinary Program in Nano-Science and Technology, Seoul National University, Seoul 151-744, Republic of Korea.
authors.forename: Homan
authors.initials: H
authors.lastname: Kang
authors.affiliation: Department of Chemistry Education, Seoul National University, Seoul 151-744, Republic of Korea.
authors.forename: Sinyoung
authors.initials: S
authors.lastname: Jeong
authors.affiliation: School of Electrical Engineering and Computer Science, Seoul National University, Seoul 151-744, Republic of Korea.
authors.forename: Yul
authors.initials: Y
authors.lastname: Koh
authors.affiliation: Department of Chemistry Education, Seoul National University, Seoul 151-744, Republic of Korea.
authors.forename: Myeong
authors.initials: M
authors.lastname: Geun Cha
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul 151-744, Republic of Korea.
authors.forename: Jin-Kyoung
authors.initials: JK
authors.lastname: Yang
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul 151-744, Republic of Korea.
authors.forename: San
authors.initials: S
authors.lastname: Kyeong
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul 151-744, Republic of Korea.
authors.forename: Jaehi
authors.initials: J
authors.lastname: Kim
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul 151-744, Republic of Korea.
authors.forename: Seon-Yeong
authors.initials: SY
authors.lastname: Kwak
authors.affiliation: Department of Chemistry Education, Seoul National University, Seoul 151-744, Republic of Korea.
authors.forename: Hye-Jin
authors.initials: HJ
authors.lastname: Chang
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul 151-744, Republic of Korea.
authors.forename: Hyunmi
authors.initials: H
authors.lastname: Lee
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul 151-744, Republic of Korea.
authors.forename: Cheolhwan
authors.initials: C
authors.lastname: Jeong
authors.affiliation: Department of Chemical Engineering, Hanyang University, Ansan, 426-791, Republic of Korea.
authors.forename: Jong-Ho
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Bong-Hyun
authors.initials: BH
authors.lastname: Jun
authors.affiliation: School of Electrical Engineering and Computer Science, Seoul National University, Seoul 151-744, Republic of Korea.
authors.forename: Yong-Kweon
authors.initials: YK
authors.lastname: Kim
authors.affiliation: Interdisciplinary Program in Nano-Science and Technology, Seoul National University, Seoul 151-744, Republic of Korea.
authors.forename: Dae
authors.initials: D
authors.lastname: Hong Jeong
authors.affiliation: 1] Interdisciplinary Program in Nano-Science and Technology, Seoul National University, Seoul 151-744, Republic of Korea [2] School of Chemical and Biological Engineering, Seoul National University, Seoul 151-744, Republic of Korea.
authors.forename: Yoon-Sik
authors.initials: YS
authors.lastname: Lee
mesh_terms: D000465:Algorithms; D008024:Ligands; D053758:Nanoparticles / Q000737:chemistry; D019151:Peptide Library; D010455:Peptides / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D012822:Silicon Dioxide / Q000737:chemistry; D013059:Spectrum Analysis, Raman
chemical_list: D008024:Ligands; D019151:Peptide Library; D010455:Peptides; D012822:Silicon Dioxide