title: [Ganoderic acid A suppresses proliferation and invasion and induces apoptosis in human osteosarcoma cells].
abstract: OBJECTIVE
  To investigate the effect of ganoderic acid A (GA-A) on the biological behaviors of human osteosarcoma cells in vitro.
  
  
  METHODS
  MG63 and HOS cells were treated with 0.1, 0.25, and 0.5 mmol/L GA-A, and the changes in cell proliferation, apoptosis and migration were evaluated using MTT assay, flow cytometry, and Transwell assay, respectively. The expressions of STAT3, p38, and NF-κB1 in the cells were analyzed by Western blotting.
  
  
  RESULTS
  GA-A effectively inhibited the proliferation of human osteosarcoma HOS and MG-63 cells in a dose-dependent manner, and induced obvious cell apoptosis in both cells. Treatment with 0.5 mmol/L GA-A also resulted in significant inhibition of the invasion of both cells. The results of Western blotting showed that GA-A down-regulated the expression level of phosphorylated STAT3 and increased the phosphorylation level of p38 and NF-κB1 expression in both cells.
  
  
  CONCLUSIONS
  GA-A can induce proliferation inhibition, apoptosis and suppression of invasion in human osteosarcoma HOS and MG-63 cells.
authors.affiliation: Department of Orthopedics and Traumatology, First Affiliated Hospital, Jinan University, Guangzhou 510632, China.E-mail: Tshaojianli@163.com.
authors.forename: Jianli
authors.initials: J
authors.lastname: Shao
authors.forename: Zhizhong
authors.initials: Z
authors.lastname: Li
authors.forename: Genlong
authors.initials: G
authors.lastname: Jiao
authors.forename: Guodong
authors.initials: G
authors.lastname: Sun
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Zhou
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001859:Bone Neoplasms / Q000473:pathology; D045744:Cell Line, Tumor / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D006538:Heptanoic Acids / Q000494:pharmacology*; D006801:Humans; D007810:Lanosterol / Q000031:analogs & derivatives* / Q000494:pharmacology; D052002:NF-kappa B p50 Subunit / Q000378:metabolism; D012516:Osteosarcoma / Q000473:pathology*; D010766:Phosphorylation; D050796:STAT3 Transcription Factor / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D006538:Heptanoic Acids; D052002:NF-kappa B p50 Subunit; C489548:NFKB1 protein, human; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D007810:Lanosterol; C515005:ganoderic acid A; D048051:p38 Mitogen-Activated Protein Kinases