title: Structural Characterization of Neutral Glycosphingolipids from 3T3-L1 Adipocytes.
abstract: In recent years, obesity has been considered a pathological stage of early lifestyle-related diseases, and adipose tissue and adipocyte research has been active. Glycosphingolipids are involved in the pathogenesis of type 2 diabetes induced by insulin resistance, but the details of the glycosphingolipid molecular species composition of adipocytes have yet to be elucidated. We used 3T3-L1 adipocytes and the 1,2-dichloroethane-wash method to remove triacylglycerols, which are abundant in adipocytes, and analyzed the structures of glycosphingolipids, particularly neutral glycosphingolipids, using liquid chromatography-mass spectrometry.
authors.affiliation: College of Life Science, Ritsumeikan University, Shiga, Japan.
authors.forename: Hisao
authors.initials: H
authors.lastname: Kojima
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ito
authors.forename: Kazuya
authors.initials: K
authors.lastname: Kabayama
mesh_terms: D041721:3T3-L1 Cells / Q000737:chemistry*; D017667:Adipocytes / Q000737:chemistry*; D000818:Animals; D002460:Cell Line; D002853:Chromatography, Liquid; D005347:Fibroblasts / Q000737:chemistry; D013058:Mass Spectrometry; D051379:Mice; D020383:Neutral Glycosphingolipids / Q000737:chemistry*
chemical_list: D020383:Neutral Glycosphingolipids