title: Dual-color three-dimensional STED microscopy with a single high-repetition-rate laser.
abstract: We describe a dual-color three-dimensional stimulated emission depletion (3D-STED) microscopy employing a single laser source with a repetition rate of 80 MHz. Multiple excitation pulses synchronized with a STED pulse were generated by a photonic crystal fiber, and the desired wavelengths were selected by an acousto-optic tunable filter with high spectral purity. Selective excitation at different wavelengths permits simultaneous imaging of two fluorescent markers at a nanoscale resolution in three dimensions.
authors.forename: Kyu Young
authors.initials: KY
authors.lastname: Han
authors.forename: Taekjip
authors.initials: T
authors.lastname: Ha
mesh_terms: D000818:Animals; D003116:Color; D021621:Imaging, Three-Dimensional / Q000295:instrumentation / Q000379:methods*; D007834:Lasers; D051379:Mice; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D041681:NIH 3T3 Cells; D014404:Tubulin / Q000737:chemistry
chemical_list: D014404:Tubulin