title: DOSY NMR and MALDI-TOF evidence of covalent binding the DNA duplex by trimethylammonium salts of topotecan upon near UV irradiation.
abstract: Using DOSY NMR and MALDI-TOF MS techniques, we present evidence that quaternary trimethylammonium salts of topotecan, [TPT-NMe3 ](+) X(-) (X = CF3SO3, HCOO), bind covalently the natural DNA oligomer upon near UV irradiation in water under physiological conditions. It is shown that formate salt is very reactive at pH 7 and requires short irradiation time. This weak irradiation at 365 nm paves the way for a new application of TPT derivatives in clinical use, which can dramatically increase the therapeutic effects of a medicine.
authors.affiliation: Institute of Organic Chemistry, Polish Academy of Sciences, 01-224, Warsaw, Kasprzaka 44, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Naumczuk
authors.affiliation: Institute of Organic Chemistry, Polish Academy of Sciences, 01-224, Warsaw, Kasprzaka 44, Poland.
authors.forename: Karolina
authors.initials: K
authors.lastname: Hyz
authors.affiliation: Institute of Organic Chemistry, Polish Academy of Sciences, 01-224, Warsaw, Kasprzaka 44, Poland.|University of Natural Sciences and Humanities, 80-110, Siedlce, Poland.
authors.forename: Robert
authors.initials: R
authors.lastname: Kawęcki
authors.affiliation: Institute of Organic Chemistry, Polish Academy of Sciences, 01-224, Warsaw, Kasprzaka 44, Poland.|National Medicines Institute, 00-725, Warsaw, Chełmska 30/34, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Bocian
authors.affiliation: National Medicines Institute, 00-725, Warsaw, Chełmska 30/34, Poland.
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Bednarek
authors.affiliation: Institute of Organic Chemistry, Polish Academy of Sciences, 01-224, Warsaw, Kasprzaka 44, Poland.|National Medicines Institute, 00-725, Warsaw, Chełmska 30/34, Poland.
authors.forename: Jerzy
authors.initials: J
authors.lastname: Sitkowski
authors.affiliation: Center of Molecular and Macromolecular Studies, Polish Academy of Sciences, Łódź, Poland.
authors.forename: Ewelina
authors.initials: E
authors.lastname: Wielgus
authors.affiliation: Institute of Organic Chemistry, Polish Academy of Sciences, 01-224, Warsaw, Kasprzaka 44, Poland.|National Medicines Institute, 00-725, Warsaw, Chełmska 30/34, Poland.
authors.forename: Lech
authors.initials: L
authors.lastname: Kozerski
mesh_terms: D001665:Binding Sites; D004247:DNA / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D012492:Salts / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D019772:Topotecan / Q000737:chemistry*; D014466:Ultraviolet Rays
chemical_list: D000644:Quaternary Ammonium Compounds; D012492:Salts; D019772:Topotecan; D004247:DNA
keywords: DNA duplex; MALDI-TOF MS; NMR; UV irradiation; covalent binding; topotecan