title: Evaluation of Factors To Determine Platelet Compatibility by Using Self-Assembled Monolayers with a Chemical Gradient.
abstract: Intercorrelation among surface chemical composition, packing structure of molecules, water contact angles, amounts and structures of adsorbed proteins, and blood compatibility was systematically investigated with self-assembled monolayers (SAMs) with continuous chemical composition gradients. The SAMs were mixtures of two thiols: n-hexanethiol (hydrophobic and protein-adsorbing) and hydroxyl-tri(ethylene glycol)-terminated alkanethiol (hydrophilic and protein-resistant) with continuously changing mixing ratios. From the systematic analyses, we found that protein adsorption is governed both by sizes of proteins and hydrophobic domains of the substrate. Furthermore, we found a clear correlation between adsorption of fibrinogen and adhesion of platelets. Combined with the results of surface force measurements, we found that the interfacial behavior of water molecules is profoundly correlated with protein resistance and antiplatelet adhesion. On the basis of these results, we conclude that the structuring of water at the SAM-water interface is a critical factor in this context.
authors.affiliation: †Department of Electronic Chemistry, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, 4259 Nagatsuta-cho Midori-ku, Yokohama, Kanagawa 226-8502, Japan.
authors.forename: Taito
authors.initials: T
authors.lastname: Sekine
authors.affiliation: †Department of Electronic Chemistry, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, 4259 Nagatsuta-cho Midori-ku, Yokohama, Kanagawa 226-8502, Japan.
authors.forename: Yusaku
authors.initials: Y
authors.lastname: Tanaka
authors.affiliation: ‡Department of Biochemical Engineering, Graduate School of Science and Engineering, Yamagata University, Jonan 4-3-16, Yonezawa 992-0038, Japan.
authors.forename: Chikako
authors.initials: C
authors.lastname: Sato
authors.affiliation: ‡Department of Biochemical Engineering, Graduate School of Science and Engineering, Yamagata University, Jonan 4-3-16, Yonezawa 992-0038, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Tanaka
authors.affiliation: †Department of Electronic Chemistry, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, 4259 Nagatsuta-cho Midori-ku, Yokohama, Kanagawa 226-8502, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Hayashi
mesh_terms: D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology*; D001792:Blood Platelets / Q000166:cytology* / Q000187:drug effects*; D001798:Blood Proteins / Q000737:chemistry; D002417:Cattle; D006046:Gold / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D010973:Platelet Adhesiveness / Q000187:drug effects; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D001798:Blood Proteins; D013438:Sulfhydryl Compounds; D006046:Gold