title: Polydopamine-coated open tubular column for the separation of proteins by capillary electrochromatography.
abstract: The separation and determination of proteins in food is an important aspect in food industry. Inspired by the self-polymerization of dopamine under alkaline conditions and the natural adhesive properties of polydopamine, in this paper, a simple and economical method was developed for the preparation of polydopamine-coated open tubular column, in which ammonium persulfate was used as the source of oxygen to induce and facilitate the polymerization of dopamine to form polydopamine. In comparison with a naked fused-silica capillary, the direction and magnitude of the electro-osmotic flow of the as-prepared polydopamine-coated open tubular column could be manipulated by varying the pH values of background solutions due to the existence of amine and phenolic hydroxyl groups on polydopamine coating. The surface morphology of the polydopamine-coated open tubular column was studied by scanning electron microscopy, and the thickness of polydopamine coating was 106 nm. The performance of the polydopamine-coated open tubular column was validated by analysis of proteins. The relative standard deviations of migration times of proteins representing run-to-run, day-to-day, and column-to-column were less than 3.5%. In addition, the feasibility of the polydopamine-coated open tubular column for real samples was verified by the separation of proteins in chicken egg white and pure milk.
authors.affiliation: Ministry of Education Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Xiao
authors.affiliation: Ministry of Education Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, China.
authors.forename: Wentao
authors.initials: W
authors.lastname: Wang
authors.affiliation: Ministry of Education Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Chen
authors.affiliation: Ministry of Education Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, China.
authors.forename: Li
authors.initials: L
authors.lastname: Jia
mesh_terms: D000818:Animals; D053801:Capillary Electrochromatography / Q000295:instrumentation / Q000379:methods*; D002417:Cattle; D004527:Egg Proteins / Q000302:isolation & purification*; D007211:Indoles / Q000737:chemistry; D008892:Milk / Q000737:chemistry; D008894:Milk Proteins / Q000302:isolation & purification*; D011108:Polymers / Q000737:chemistry
chemical_list: D004527:Egg Proteins; D007211:Indoles; D008894:Milk Proteins; D011108:Polymers; C568283:polydopamine
keywords: Capillary electrochromatography; Open tubular column; Polydopamine; Protein separation