title: Genotyping ofListeria monocytogenesisolates from poultry carcasses using high resolution melting (HRM) analysis.
abstract: An outbreak situation of human listeriosis requires a fast and accurate protocol for typingListeria monocytogenes. Existing techniques are either characterized by low discriminatory power or are laborious and require several days to give a final result. Polymerase chain reaction (PCR) coupled with high resolution melting (HRM) analysis was investigated in this study as an alternative tool for a rapid and precise genotyping ofL. monocytogenesisolates. Fifty-five isolates ofL. monocytogenesisolated from poultry carcasses and the environment of four slaughterhouses were typed by HRM analysis using two specific markers,internalin BandssrAgenes. The analysis of genotype confidence percentage ofL. monocytogenesisolates produced by HRM analysis generated dendrograms with two major groups and several subgroups. Furthermore, the analysis of the HRM curves revealed that allL. monocytogenesisolates could easily be distinguished. In conclusion, HRM was proven to be a fast and powerful tool for genotyping isolates ofL. monocytogenes.
authors.affiliation: Institute of Applied Biosciences, Centre for Research and Technology Hellas , Thessaloniki , Greece.
authors.forename: Ioannis
authors.initials: I
authors.lastname: Sakaridis
authors.affiliation: Institute of Applied Biosciences, Centre for Research and Technology Hellas , Thessaloniki , Greece.
authors.forename: Ioannis
authors.initials: I
authors.lastname: Ganopoulos
authors.affiliation: Institute of Applied Biosciences, Centre for Research and Technology Hellas , Thessaloniki , Greece.
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Madesis
authors.affiliation: Institute of Applied Biosciences, Centre for Research and Technology Hellas , Thessaloniki , Greece.
authors.forename: Athanasios
authors.initials: A
authors.lastname: Tsaftaris
authors.affiliation: Institute of Applied Biosciences, Centre for Research and Technology Hellas , Thessaloniki , Greece.
authors.forename: Anagnostis
authors.initials: A
authors.lastname: Argiriou
keywords: HRM; L. monocytogenes; RAPD; genotyping; poultry carcasses