title: MicroRNA-24 Regulates Osteogenic Differentiation via Targeting T-Cell Factor-1.
abstract: MicroRNAs (miRNAs) have been reported to have diverse biological roles in regulating many biological processes, including osteogenic differentiation. In the present study, we identified that miR-24 was a critical regulator during osteogenic differentiation. We found that overexpression of miR-24 significantly inhibited osteogenic differentiation, which decreased alkaline phosphatase activity, matrix mineralization and the expression of osteogenic differentiation markers. In contrast, inhibition of miR-24 exhibited an opposite effect. Furthermore, we delineated that miR-24 regulates post-transcriptionals of T-cell factor-1 (Tcf-1) via targeting the 3'-untranslated region (UTR) of Tcf-1 mRNA. MiR-24 was further found to regulate the protein expression of Tcf-1 in the murine osteoprogenitors cells and bone mesenchymal stem cells. Additionally, the positive effect of miR-24 suppression on osteoblast differentiation was apparently abrogated by Tcf-1 silencing. Taken together, our data suggest that miR-24 participates in osteogenic differentiation by targeting and regulating Tcf-1 expression in osteoblastic cells.
authors.affiliation: Department of Orthopedics, the First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710061, China. weigongzhao@126.com.
authors.forename: Weigong
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Immunology and Pathogenic Biology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an 710061, China. caijunwucc@163.com.
authors.forename: Caijun
authors.initials: C
authors.lastname: Wu
authors.affiliation: Department of Immunology and Pathogenic Biology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an 710061, China. yanyingdong11@163.com.
authors.forename: Yanying
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Department of Immunology and Pathogenic Biology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an 710061, China. yunfengma126@163.com.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Pathology, the Second Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710004, China. yaofengjin@126.com.
authors.forename: Yaofeng
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Department of Immunology and Pathogenic Biology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an 710061, China. yanhongjixian@163.com.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Ji
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D005786:Gene Expression Regulation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D010012:Osteogenesis; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D051822:T Cell Transcription Factor 1 / Q000235:genetics* / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; D035683:MicroRNAs; C529080:Mirn24 microRNA, mouse; D012333:RNA, Messenger; D051822:T Cell Transcription Factor 1
keywords: T-cell factor-1; differentiation; miR-24; osteoblast