title: The plasma proteomic signature as a strategic tool for early diagnosis of acute coronary syndrome.
abstract: BACKGROUND
  Acute coronary syndrome is the major cause of death in developed countries. Despite its high prevalence, there is still a strong need for new biomarkers which permit faster and more accurate diagnostics and new therapeutic drugs. The basis for this challenge lay in improving our understanding of the whole atherosclerotic process from atherogenesis to atherothrombosis. In this study, we conducted two different proteomic analyses of peripheral blood plasma from non-ST elevation acute coronary syndrome and ST elevation acute coronary syndrome patients vs healthy controls.
  
  
  RESULTS
  Two-dimensional Fluorescence Difference in Gel Electrophoresis and mass spectrometry permitted the identification of 31 proteins with statistical differences (p < 0.05) between experimental groups. Additionally, validation by Western blot and Selected Reaction Monitoring permitted us to confirm the identification of a different and characteristic plasma proteomic signature for NSTEACS and STEACS patients.
  
  
  CONCLUSIONS
  We purpose the severity of hypoxia as the cornerstone for explaining the differences observed between both groups.
authors.affiliation: Laboratory of Vascular Physiopathology, Hospital Nacional de Parapléjicos, SESCAM, Toledo, Spain.
authors.forename: Carlos M
authors.initials: CM
authors.lastname: Laborde
authors.affiliation: Laboratory of Vascular Physiopathology, Hospital Nacional de Parapléjicos, SESCAM, Toledo, Spain.
authors.forename: Sergio
authors.initials: S
authors.lastname: Alonso-Orgaz
authors.affiliation: Laboratory of Vascular Physiopathology, Hospital Nacional de Parapléjicos, SESCAM, Toledo, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Mourino-Alvarez
authors.affiliation: Department of Hemodynamic, Hospital Virgen de la Salud, SESCAM, Toledo, Spain.
authors.forename: José
authors.initials: J
authors.lastname: Moreu
authors.affiliation: Department of Immunology, IIS-Fundación Jiménez Diaz, Madrid, Spain ; Department of Biochemistry and Molecular Biology I, Universidad Complutense, Madrid, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: Vivanco
authors.affiliation: Department of Cardiology, Hospital Virgen de la Salud, Toledo, Spain.
authors.forename: Luis R
authors.initials: LR
authors.lastname: Padial
authors.affiliation: Laboratory of Vascular Physiopathology, Hospital Nacional de Parapléjicos, SESCAM, Toledo, Spain.
authors.forename: María G
authors.initials: MG
authors.lastname: Barderas
keywords: Acute coronary syndrome; Biomarker; Protein; Two-dimensional fluorescence difference in gel electrophoresis