title: Comparative Study of Metal Quantification in Neurological Tissue Using Laser Ablation-Inductively Coupled Plasma-Mass Spectrometry Imaging and X-ray Fluorescence Microscopy.
abstract: Redox-active metals in the brain mediate numerous biochemical processes and are also implicated in a number of neurodegenerative diseases. A number of different approaches are available for quantitatively measuring the spatial distribution of biometals at an image resolution approaching the subcellular level. Measured biometal levels obtained using laser ablation-inductively coupled plasma mass spectrometry (LA-ICPMS; spatial resolution 15 μm × 15 μm) were within the range of those obtained using X-ray fluorescence microscopy (XFM; spatial resolution 2 μm × 7 μm) and regional changes in metal concentration across discrete brain regions were replicated to the same degree. Both techniques are well suited to profiling changes in regional biometal distribution between healthy and diseased brain tissues, but absolute quantitation of metal levels varied significantly between methods, depending on the metal of interest. Where all possible variables affect metal levels, independent of a treatment/phenotype are controlled, either method is suitable for examining differences between experimental groups, though, as with any method for imaging post mortem brain tissue, care should be taken when interpreting the total metal levels with regard to physiological concentrations.
authors.affiliation: †Neuroscience Research Australia, Randwick, New South Wales, Australia.|‡School of Medical Sciences, Faculty of Medicine, University of New South Wales, Kensington, New South Wales, Australia.
authors.forename: Katherine M
authors.initials: KM
authors.lastname: Davies
authors.affiliation: §Elemental Bio-imaging Facility, University of Technology Sydney, Broadway, New South Wales, Australia.|∥The Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Parkville, Victoria, Australia.|⊥Senator Frank R. Lautenberg Environmental Health Sciences Laboratory, Department of Preventive Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, United States.
authors.forename: Dominic J
authors.initials: DJ
authors.lastname: Hare
authors.affiliation: #Inserm, U836, Team 6, Rayonnement Synchrotron et Recherche Médicales, Grenoble Institut des Neurosciences, Grenoble, France.|⊗European Synchrotron Radiation Facility, BP220, Grenoble, France.|○Université Joseph Fourier 1, Grenoble Institut des Neurosciences, Grenoble, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Bohic
authors.affiliation: $Australian Synchrotron, Clayton, Victoria, Australia.|¶Materials Science and Engineering, Commonwealth Scientific and Industrial Research Organisation, Clayton, Victoria, Australia.
authors.forename: Simon A
authors.initials: SA
authors.lastname: James
authors.affiliation: ∥The Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Jessica L
authors.initials: JL
authors.lastname: Billings
authors.affiliation: ∥The Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Parkville, Victoria, Australia.
authors.forename: David I
authors.initials: DI
authors.lastname: Finkelstein
authors.affiliation: §Elemental Bio-imaging Facility, University of Technology Sydney, Broadway, New South Wales, Australia.
authors.forename: Philip A
authors.initials: PA
authors.lastname: Doble
authors.affiliation: †Neuroscience Research Australia, Randwick, New South Wales, Australia.|×Brain and Mind Research Institute, The University of Sydney, 94-100 Mallett Street,Camperdown, New South Wales, Australia.
authors.forename: Kay L
authors.initials: KL
authors.lastname: Double
mesh_terms: D000818:Animals; D007834:Lasers; D013058:Mass Spectrometry / Q000379:methods*; D008670:Metals / Q000032:analysis*; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence / Q000379:methods*; D009420:Nervous System / Q000737:chemistry*
chemical_list: D008670:Metals