title: Allelic Discrimination by TaqMan-PCR for Genotyping of Human Neutrophil Antigens.
abstract: Neutrophil antigens are implicated in a variety of clinical conditions, including neonatal immune neutropenia, transfusion-related acute lung injury, refractoriness to granulocyte transfusions, febrile transfusion reactions, and autoimmune neutropenia. In this report, we describe simultaneous genotyping of human neutrophil antigens (HNA)-1, -3, -4, and -5 using PCR with allele-specific TaqMan probes and end-point fluorescence detection, which is a robust, rapid, and reproducible method, allowing for high-throughput genotyping.
authors.affiliation: Department of Clinical Immunology, Aalborg Hospital, Aalborg, 9100, Denmark, rns@rn.dk.
authors.forename: Rudi
authors.initials: R
authors.lastname: Steffensen
authors.forename: John
authors.initials: J
authors.lastname: Baech
authors.forename: Kaspar R
authors.initials: KR
authors.lastname: Nielsen
mesh_terms: D000483:Alleles; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005838:Genotype; D060005:Genotyping Techniques / Q000191:economics / Q000379:methods*; D006801:Humans; D007519:Isoantigens / Q000235:genetics*; D009504:Neutrophils / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods*
chemical_list: C518134:HNA-3a antigen, human; C531098:HNA-4A antigen; D007519:Isoantigens; C067704:neutrophil-specific antigen NA1, human; D004247:DNA