title: Phase and Texture Characterizations of Scar Collagen Second-Harmonic Generation Images Varied with Scar Duration.
abstract: This work developed a phase congruency algorithm combined with texture analysis to quantitatively characterize collagen morphology in second-harmonic generation (SHG) images from human scars. The extracted phase and texture parameters of the SHG images quantified collagen directionality, homogeneity, and coarseness in scars and varied with scar duration. Phase parameters showed an increasing tendency of the mean of phase congruency with scar duration, indicating that collagen fibers are better oriented over time. Texture parameters calculated from local difference local binary pattern (LD-LBP) and Haar wavelet transform, demonstrated that the LD-LBP variance decreased and the energy of all subimages increased with scar duration. It implied that collagen has a more regular pattern and becomes coarser with scar duration. In addition, the random forest regression was used to predict scar duration, demonstrating reliable performance of the extracted phase and texture parameters in characterizing collagen morphology in scar SHG images. Results indicate that the extracted parameters using the proposed method can be used as quantitative indicators to monitor scar progression with time and can help understand the mechanism of scar progression.
authors.affiliation: 1Fujian Provincial Key Laboratory for Photonics Technology,Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education,Institute of Laser and Optoelectronics Technology,Fujian Normal University,Fuzhou 350007,PR China.
authors.forename: Guannan
authors.initials: G
authors.lastname: Chen
authors.affiliation: 1Fujian Provincial Key Laboratory for Photonics Technology,Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education,Institute of Laser and Optoelectronics Technology,Fujian Normal University,Fuzhou 350007,PR China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Liu
authors.affiliation: 1Fujian Provincial Key Laboratory for Photonics Technology,Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education,Institute of Laser and Optoelectronics Technology,Fujian Normal University,Fuzhou 350007,PR China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Zhu
authors.affiliation: 1Fujian Provincial Key Laboratory for Photonics Technology,Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education,Institute of Laser and Optoelectronics Technology,Fujian Normal University,Fuzhou 350007,PR China.
authors.forename: Zufang
authors.initials: Z
authors.lastname: Huang
authors.affiliation: 1Fujian Provincial Key Laboratory for Photonics Technology,Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education,Institute of Laser and Optoelectronics Technology,Fujian Normal University,Fuzhou 350007,PR China.
authors.forename: Jianyong
authors.initials: J
authors.lastname: Cai
authors.affiliation: 1Fujian Provincial Key Laboratory for Photonics Technology,Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education,Institute of Laser and Optoelectronics Technology,Fujian Normal University,Fuzhou 350007,PR China.
authors.forename: Rong
authors.initials: R
authors.lastname: Chen
authors.affiliation: 4Department of Plastic Surgery,Affiliated First Hospital Fujian Medical University,Fuzhou 350005,PR China.
authors.forename: Shuyuan
authors.initials: S
authors.lastname: Xiong
authors.affiliation: 3Imaging Unit-Integrative Oncology Department,British Columbia Cancer Agency Research Centre,675 West 10th Avenue,Vancouver,BC V5Z 1L3,Canada.
authors.forename: Haishan
authors.initials: H
authors.lastname: Zeng
mesh_terms: D002921:Cicatrix / Q000473:pathology*; D003094:Collagen / Q000032:analysis* / Q000648:ultrastructure*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D061848:Optical Imaging; D013997:Time Factors
chemical_list: D003094:Collagen
keywords: collagen; phase parameter; scar progression; second harmonic generation (SHG) image; texture parameter