title: Detergent-like stressor and nutrient in metabolism ofPenicillium chrysogenum.
abstract: The influence of detergents on the metabolism ofPenicillium chrysogenumfrom two aspects, as a stress factor and potential nutrient, was studied. The fungus was isolated from the river bed Lepenica, Kragujevac, at a place where sewage domestic wastewater discharged into the river. The fungus was grown in a liquid nutrient medium according to Czapek with and without addition of commercial detergent (MERIX, Henkel, Serbia) at a concentration of 0.3% and 0.5%. The biochemical changes of pH, redox potential, free and total organic acids, total dry weight biomass, activity of alkaline and acid invertase and alkaline phosphatase were evaluated from day 3 to day 16 of the fungus growth. At the same time, detergent disappearance in terms of methylene blue active substances in the medium was measured. The detergent at a concentration of 0.5% showed a fungicide effect. In the medium with 0.3% of detergent, there was increased pH and concentration of organic acids, but decreased redox potential and total dry weight biomass. The detergent also showed an inhibitory effect on invertase and phosphatase activity.P. chrysogenumdecomposed 50.2% of the total detergent concentration for an experimental period of 16 days.
authors.affiliation: Institute for Biology and Ecology, Faculty of Science, University of Kragujevac , Kragujevac , Serbia.
authors.forename: Violeta
authors.initials: V
authors.lastname: Jakovljević
authors.affiliation: Institute for Biology and Ecology, Faculty of Science, University of Kragujevac , Kragujevac , Serbia.
authors.forename: Jasmina
authors.initials: J
authors.lastname: Milićević
authors.affiliation: Institute for Biology and Ecology, Faculty of Science, University of Kragujevac , Kragujevac , Serbia.
authors.forename: Jelica
authors.initials: J
authors.lastname: Stojanović
keywords: biodegradation of detergent; biomass; enzyme activity; organic acids; pH; redox potential