title: Effect of 17α-hydroxyprogesterone and 17α-hydroxypregnenolone on sperm quality and sperm quantity in male mud spiny lobster (Panulirus polyphagus).
abstract: The present study aimed to determine the effect of 17α-hydroxyprogesterone (17α-OHP) and 17α-hydroxypregnenolone (17α-OHPL) on sperm quality and sperm quantity in male mud spiny lobster (Panulirus polyphagus). The mean of sperm quality was increased in 17α-OHP and 17α-OHPL treated hormones. In 17α-OHP injected animals, the mean of sperm quantity of dose 0.01 μg g(-1) b.wt. was increased than 17α-OHPL. Meanwhile, 17α-OHP and 17α-OHPL concentrations were lower when injected with the hormones but 17α-OHP was higher at only day 15 (dose 0.01 and 0.1 μg g(-1) b.wt.). For 17α-OHPL, the hormone was a prohormone in the body of P. polyphagus and only required smaller to increase the sperm quantity. Besides, when the higher dose of 17α-OHPL (0.1 μg g(-1) b.wt.) was used in P. polyphagus, the development of P. polyphagus was inhibited and decreased the sperm quantity and 17α-OHPL concentration in hemolymph was lower. Injection of 17α-OHP in P. polyphagus has increased the sperm quality and quantity for both 17α-OHP dosage of 0.01 and 0.1 μg g(-1) b.wt. However, injection of 17α-OHPL in P. polyphagus has decreased the sperm quantity only, also for both dosage of 0.01 and 0.1 μg g(-1) b.wt. and lower on hormone concentration.
authors.forename: S N
authors.initials: SN
authors.lastname: Fatihah
authors.forename: J
authors.initials: J
authors.lastname: Safiah
authors.forename: A B
authors.initials: AB
authors.lastname: Abol-Munafi
authors.forename: M
authors.initials: M
authors.lastname: Ikhwanuddin
mesh_terms: D006907:17-alpha-Hydroxypregnenolone / Q000494:pharmacology*; D019326:17-alpha-Hydroxyprogesterone / Q000494:pharmacology*; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D008297:Male; D033521:Palinuridae / Q000187:drug effects* / Q000502:physiology; D013076:Sperm Count; D013091:Spermatogenesis / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology; D013997:Time Factors
chemical_list: D006907:17-alpha-Hydroxypregnenolone; D019326:17-alpha-Hydroxyprogesterone