title: Phosphoproteomic analyses of L-02 liver cells exposed to trichloroethylene.
abstract: Trichloroethylene (TCE) is an environmental and occupational toxicant that has been shown to cause serious hepatotoxicity. However, the mechanisms underlying the hepatotoxicity of TCE remain unclear. Previously, we identified several apoptosis-related proteins in TCE-induced hepatic cytotoxicity. This study is aimed to analyze the changes in phosphoproteins in L-02 liver cells exposed to TCE using iTRAQ labeling, IMAC enrichment and LC-MS/MS. We identified 1878 phosphorylation sites in 107 proteins and found that 20 sites in 16 phosphoproteins were differentially phosphorylated in L-02 cells after TCE treatment. Among these phosphoproteins, 20% were protein localization and formation processes-related proteins, 38% were metabolism-related proteins and 42% were cellular process-related proteins, including transcriptional regulation and biogenesis. Moreover, two phosphoproteins, 4E-BP1 (37T) and MCM2 (139S), were validated as TCE-induced alteration of phosphorylation at specific sites by Western-blot analysis. Taken together, our study demonstrated that TCE exposure changed the levels of multiple phosphoproteins in L-02 liver cells, and the functional analysis suggested that these differentially expressed phosphoproteins might be involved in TCE-induced hepatic cytotoxicity.
authors.affiliation: a School of Public Health and Tropical Medicine, Southern Medical University , Tonghe District , Guangzhou , P.R. China .|b Key Laboratory of Modern Toxicology of Shenzhen , Medical Key Laboratory of Shenzhen Center for Disease Control and Prevention , Nanshan District , Shenzhen , P.R. China , and.
authors.forename: Xiaohu
authors.initials: X
authors.lastname: Ren
authors.affiliation: a School of Public Health and Tropical Medicine, Southern Medical University , Tonghe District , Guangzhou , P.R. China .
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: a School of Public Health and Tropical Medicine, Southern Medical University , Tonghe District , Guangzhou , P.R. China .|b Key Laboratory of Modern Toxicology of Shenzhen , Medical Key Laboratory of Shenzhen Center for Disease Control and Prevention , Nanshan District , Shenzhen , P.R. China , and.
authors.forename: Bo
authors.initials: B
authors.lastname: Xia
authors.affiliation: b Key Laboratory of Modern Toxicology of Shenzhen , Medical Key Laboratory of Shenzhen Center for Disease Control and Prevention , Nanshan District , Shenzhen , P.R. China , and.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: b Key Laboratory of Modern Toxicology of Shenzhen , Medical Key Laboratory of Shenzhen Center for Disease Control and Prevention , Nanshan District , Shenzhen , P.R. China , and.
authors.forename: Xifei
authors.initials: X
authors.lastname: Yang
authors.affiliation: b Key Laboratory of Modern Toxicology of Shenzhen , Medical Key Laboratory of Shenzhen Center for Disease Control and Prevention , Nanshan District , Shenzhen , P.R. China , and.
authors.forename: Wen-Xu
authors.initials: WX
authors.lastname: Hong
authors.affiliation: b Key Laboratory of Modern Toxicology of Shenzhen , Medical Key Laboratory of Shenzhen Center for Disease Control and Prevention , Nanshan District , Shenzhen , P.R. China , and.
authors.forename: Peiwu
authors.initials: P
authors.lastname: Huang
authors.affiliation: c School of Life Sciences, Shenzhen University , Shenzhen , P.R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: c School of Life Sciences, Shenzhen University , Shenzhen , P.R. China.
authors.forename: Shuiming
authors.initials: S
authors.lastname: Li
authors.affiliation: a School of Public Health and Tropical Medicine, Southern Medical University , Tonghe District , Guangzhou , P.R. China .
authors.forename: Fei
authors.initials: F
authors.lastname: Zou
authors.affiliation: a School of Public Health and Tropical Medicine, Southern Medical University , Tonghe District , Guangzhou , P.R. China .|b Key Laboratory of Modern Toxicology of Shenzhen , Medical Key Laboratory of Shenzhen Center for Disease Control and Prevention , Nanshan District , Shenzhen , P.R. China , and.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Liu
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D002460:Cell Line; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology* / Q000378:metabolism; D002853:Chromatography, Liquid; D004785:Environmental Pollutants / Q000633:toxicity*; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D040901:Proteomics / Q000379:methods; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry; D014241:Trichloroethylene / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D004785:Environmental Pollutants; D010750:Phosphoproteins; D014241:Trichloroethylene
keywords: 4E-BP1; MCM2; hepatic cytoxicity; phosphoproteomic; trichloe