title: Immobilization of enzymes via microcontact printing and thiol-ene click chemistry.
abstract: This Communication describes a bioconjugation method for the generation of enzyme microarrays on surfaces using photochemical thiol-ene chemistry in combination with microcontact printing. Glucose oxidase and lactase were readily immobilized (i.e., printing time 2 min) on alkene terminated self-assembled monolayers on glass as demonstrated by X-ray photoelectron spectroscopy and fluorescence microscopy. Furthermore, the activity of both immobilized enzymes was confirmed in single enzyme as well as cascade transformations.
authors.affiliation: Organic Chemistry Institute and Center for Soft Nanoscience, Westfälische Wilhelms-Universität Münster, Corrensstrasse 40, 48149 Münster, Germany.
authors.forename: Moritz
authors.initials: M
authors.lastname: Buhl
authors.affiliation: Organic Chemistry Institute and Center for Soft Nanoscience, Westfälische Wilhelms-Universität Münster, Corrensstrasse 40, 48149 Münster, Germany.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Vonhören
authors.affiliation: Organic Chemistry Institute and Center for Soft Nanoscience, Westfälische Wilhelms-Universität Münster, Corrensstrasse 40, 48149 Münster, Germany.
authors.forename: Bart Jan
authors.initials: BJ
authors.lastname: Ravoo
mesh_terms: D000475:Alkenes / Q000737:chemistry; D057930:Click Chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D004867:Equipment Design; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D043322:Lactase / Q000737:chemistry / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008958:Models, Molecular; D056951:Photoelectron Spectroscopy; D040081:Protein Array Analysis; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D000475:Alkenes; D004800:Enzymes, Immobilized; D013438:Sulfhydryl Compounds; D005949:Glucose Oxidase; D043322:Lactase