title: Parallelized ultra-high throughput microfluidic emulsifier for multiplex kinetic assays.
abstract: Droplet-based microfluidic technologies are powerful tools for applications requiring high-throughput, for example, in biochemistry or material sciences. Several systems have been proposed for the high-throughput production of monodisperse emulsions by parallelizing multiple droplet makers. However, these systems have two main limitations: (1) they allow the use of only a single disperse phase; (2) they are based on multiple layer microfabrication techniques. We present here a pipette-and-play solution offering the possibility of manipulating simultaneously 10 different disperse phases on a single layer device. This system allows high-throughput emulsion production using aqueous flow rates of up to 26 ml/h (>110 000 drops/s) leading to emulsions with user-defined complex chemical composition. We demonstrate the multiplex capabilities of our system by measuring the kinetics of β-galactosidase in droplets using nine different concentrations of a fluorogenic substrate.
authors.forename: Jiseok
authors.initials: J
authors.lastname: Lim
authors.forename: Ouriel
authors.initials: O
authors.lastname: Caen
authors.forename: Jérémy
authors.initials: J
authors.lastname: Vrignon
authors.affiliation: Max Planck Institute for Biophysical Chemistry , Am Fassberg 11, 37077 Goettingen, Germany.
authors.forename: Manfred
authors.initials: M
authors.lastname: Konrad
authors.affiliation: Université Paris Sorbonne Cité , INSERM UMR-S1147, Centre Universitaire des Saints-Pères, 45 rue des Saints-Pères, 75270 Paris Cedex 06, France.
authors.forename: Valérie
authors.identifier: 0000-0002-3116-8602
authors.initials: V
authors.lastname: Taly
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Baret