title: RhoE is required for contact inhibition and negatively regulates tumor initiation and progression.
abstract: RhoE is a small GTPase involved in the regulation of actin cytoskeleton dynamics, cell cycle and apoptosis. The role of RhoE in cancer is currently controversial, with reports of both oncogenic and tumor-suppressive functions for RhoE. Using RhoE-deficient mice, we show here that the absence of RhoE blunts contact-inhibition of growth by inhibiting p27Kip1 nuclear translocation and cooperates in oncogenic transformation of mouse primary fibroblasts. Heterozygous RhoE+/gt mice are more susceptible to chemically induced skin tumors and RhoE knock-down results in increased metastatic potential of cancer cells. These results indicate that RhoE plays a role in suppressing tumor initiation and progression.
authors.affiliation: Universidad CEU-Cardenal Herrera, Facultad de Ciencias de la Salud, Dep. Ciencias Biomédicas, Moncada, Spain.|Departament de Biologia Cellular, Fisiologia i Immunologia, Universitat Autònoma de Barcelona, Cerdanyola del Vallés, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Hernández-Sánchez
authors.affiliation: Universidad CEU-Cardenal Herrera, Facultad de Ciencias de la Salud, Dep. Ciencias Biomédicas, Moncada, Spain.
authors.forename: Enric
authors.initials: E
authors.lastname: Poch
authors.affiliation: Centro de Investigación Príncipe Felipe, Rho Signaling in Neuropathologies, Valencia, Spain.
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Guasch
authors.affiliation: Universidad CEU-Cardenal Herrera, Facultad de Veterinaria, Dep. PASACTA, Moncada, Spain.
authors.forename: Joaquín
authors.initials: J
authors.lastname: Ortega
authors.affiliation: Universidad CEU-Cardenal Herrera, Facultad de Ciencias de la Salud, Dep. Ciencias Biomédicas, Moncada, Spain.
authors.forename: Inmaculada
authors.initials: I
authors.lastname: López-Almela
authors.affiliation: Instituto de Investigaciones Biomédicas "Alberto Sols" CSIC-UAM, Madrid, Spain.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Palmero
authors.affiliation: Universidad CEU-Cardenal Herrera, Facultad de Ciencias de la Salud, Dep. Ciencias Biomédicas, Moncada, Spain.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Pérez-Roger
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002471:Cell Transformation, Neoplastic / Q000378:metabolism* / Q000473:pathology; D003260:Contact Inhibition / Q000502:physiology*; D018450:Disease Progression; D007150:Immunohistochemistry; D051379:Mice; D018345:Mice, Knockout; D008819:Mice, Nude; D009374:Neoplasms, Experimental / Q000378:metabolism / Q000473:pathology*; D020741:rho GTP-Binding Proteins / Q000378:metabolism*
chemical_list: C513341:Rnd3 protein, mouse; D020741:rho GTP-Binding Proteins
keywords: RhoE; contact inhibition; metastasis; p27Kip1; tumor suppression