title: [Effect of artenisiae scopariae and poriae powder on calpain-2 expression in liver tissue from rats with obstructive jaundice].
abstract: OBJECTIVE
  To explore the eff ect of artenisiae scopariae and poriae powder (ASPD) on calpain-2 expression in liver tissue from rats with obstructive jaundice.
  
  
  METHODS
  The rat model of obstructive jaundice was established. SD rats was divided into the control group, the obstructive jaundice group, the obstructive jaundice model plus ASPD group, the obstructive jaundice model plus saline group. Th e serum levels of TBIL, ALT, AST and other biochemical indexes were detected. The pathological changes of liver tissue were evaluated by HE staining. The calpain-2 mRNA and protein expression in liver was measured by Real-time PCR and immunohistochemistry or Western blot, respectively.
  
  
  RESULTS
  The calpain-2 mRNA and protein expression levels were significantly up-regulated in live tissues from the rats with obstructive jaundice in a time-dependent manner. The ASPD could inhibit the calpain-2 expression in rats with obstructive jaundice concomitant with the decreased liver damage and the improved liver function, suggesting that calpain-2 was involved in endoplasmic reticulum stress-mediated cellular apoptosis and the occurrence of obstructive jaundice.
  
  
  CONCLUSIONS
  ASPD could be used for patients with obstructive jaundice to promote the recovery of liver function after operation and to reduce the incidence of complications, which provide a theoretical basis for the reasonable application of traditional Chinese medicine in the peroperative period.
authors.affiliation: Department of Surgery, Tianjin First Hospital, Tianjin 300232, China cuiyubao022@163.com.
authors.forename: Yubao
authors.initials: Y
authors.lastname: Cui
mesh_terms: D000818:Animals; D017209:Apoptosis; D019306:Artemisia / Q000737:chemistry; D002154:Calpain / Q000378:metabolism*; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal; D041781:Jaundice, Obstructive / Q000201:enzymology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D002154:Calpain; C000594975:Capn2 protein, rat