title: Immune activity of sweet potato (Ipomoea batatas L.) glycoprotein after enzymatic and chemical modifications.
abstract: This study aimed to investigate the immune activity of sweet potato (Ipomoea batatas L.) glycoprotein (SPG-1) before and after enzymatic and chemical modifications. The protein portion of SPG-1 was modified by pepsin, trypsin, and acetylation treatments. The carbohydrate portion was modified by glucoamylase, NaIO4, and sulfation treatments. The carbohydrate chain of SPG-1 (SPG-1-C) was obtained. Immune activity was analyzed by measuring the serum lysozyme activity and T cell immune response. SPG-1 increased immune activity with a dose-response effect. Immune activity was slightly decreased after pepsin and trypsin hydrolysis, whereas it increased after a moderate degree (DS = 0.68) of acetylation. Immune activity was partly decreased after glucoamylase hydrolysis, remarkably decreased after NaIO4 oxidation, or was lost after a high modification by sulfation. Compared with SPG-1 groups, the SPG-1-C groups increased immune activities had insignificant (P > 0.05) differences. Hence, appropriate modifications of the protein portion could be conducted and it was found that high modifications of the carbohydrate portion should be avoided to improve or maintain the immune function of SPG-1.
authors.affiliation: College of Food Science, Southwest University, Chongqing 400715, China. kanjianquan@163.com.
authors.forename: Xuejuan
authors.initials: X
authors.lastname: Xia
authors.forename: Guannan
authors.initials: G
authors.lastname: Li
authors.forename: Jiong
authors.initials: J
authors.lastname: Zheng
authors.forename: Jinsong
authors.initials: J
authors.lastname: Wu
authors.forename: Jianquan
authors.initials: J
authors.lastname: Kan
mesh_terms: D000107:Acetylation; D000818:Animals; D055598:Chemical Phenomena; D002681:China; D004044:Dietary Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D055808:Drug Discovery; D005260:Female; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D006868:Hydrolysis; D007113:Immunity, Innate / Q000187:drug effects*; D007155:Immunologic Factors / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D027723:Ipomoea batatas / Q000737:chemistry*; D008297:Male; D008807:Mice, Inbred BALB C; D009113:Muramidase / Q000097:blood / Q000378:metabolism; D010084:Oxidation-Reduction; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D035281:Plant Tubers / Q000737:chemistry*; D059748:Proteolysis; D011897:Random Allocation; D058248:Serum Bactericidal Antibody Assay; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology
chemical_list: D004044:Dietary Proteins; D006023:Glycoproteins; D007155:Immunologic Factors; D010940:Plant Proteins; D009113:Muramidase