title: Short term ex vivo storage of kidneys cause progressive nuclear ploidy changes of renal tubular epitheliocytes.
abstract: In renal transplantation, there has been considerable success, mainly in term of post-transplant graft function. However, upon closer scrutiny, it is known that severe dysfunction, including persistence of renal failure is seen after transplantation. The major condition that potentially cause significant lesion may be hypothesized to be related to the hypothermic approach to storage. To systematically examine these issues, we stored mammalian (sheep) kidneys in UWS at 4 °C for four different time points (0, 1, 3 and 6 hours). We obtained renal histological sections and examined tubular architecture as well as nuclear characteristics of tubular epitheliocytes. The results of our preliminary investigations suggest that there are temporal changes of tubular epitheliocytes, as well as genomic changes. These changes were also seen in tissues stored at room temperature. Our observations suggest the need for additional studies for redesigning of improvised storage solutions. Pilot studies using Celsior also revealed similar kind of nuclear changes, suggesting that storage conditions are contributory, including perfusion versus static conditions. The results may explain persistence of tubular injury several days after orthotopic transplantation, and may potentially be contributory to delayed graft function (DGF).
authors.affiliation: Department of Hemodialysis, Qilu Hospital, Shandong University, Jinan 250012,China.
authors.forename: Huaibin
authors.initials: H
authors.lastname: Sun
authors.affiliation: Department of Hemodialysis, Qilu Hospital, Shandong University, Jinan 250012,China.
authors.forename: Jun
authors.initials: J
authors.lastname: Tian
authors.affiliation: Department of Hemodialysis, Qilu Hospital, Shandong University, Jinan 250012,China.
authors.forename: Wanhua
authors.initials: W
authors.lastname: Xian
authors.affiliation: Department of Nephrology, Qilu Hospital, Shandong University, Jinan 250012, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Xie
authors.affiliation: Department of Nephrology, Qilu Hospital, Shandong University, Jinan 250012, China.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Yang
mesh_terms: D018262:Adenocarcinoma, Clear Cell / Q000473:pathology; D000818:Animals; D002467:Cell Nucleus / Q000473:pathology; D004847:Epithelial Cells / Q000473:pathology*; D006801:Humans; D007680:Kidney Neoplasms / Q000473:pathology; D016030:Kidney Transplantation; D007684:Kidney Tubules / Q000473:pathology*; D008297:Male; D009926:Organ Preservation / Q000009:adverse effects*; D011003:Ploidies; D012756:Sheep; D013696:Temperature; D013997:Time Factors