title: DNA Microarray-Based Identification of Genes Regulated by NtrC in Bradyrhizobium japonicum.
abstract: The Bradyrhizobium japonicum NtrBC two-component system is a critical regulator of cellular nitrogen metabolism, including the acquisition and catabolism of nitrogenous compounds. To better define the roles of this system, genome-wide transcriptional profiling was performed to identify the NtrC regulon during the response to nitrogen limitation. Upon cells perceiving low intracellular nitrogen, they stimulate the phosphorylation of NtrC, which induces genes responsible for alteration of the core glutamine synthetase/glutamate synthase nitrogen assimilation pathway, including the genes for the glutamine synthetases and PII proteins. In addition, genes responsible for the import and utilization of multiple nitrogen sources, specifically nitrate and nitrite, were upregulated by NtrC activation. Mutational analysis of a candidate nitrite reductase revealed a role for NtrC in regulating the assimilation of nitrite, since mutations in both ntrC and the gene encoding the candidate nitrite reductase abolished the ability to grow on nitrite as a sole nitrogen source.
authors.affiliation: National Center for Soybean Biotechnology, Division of Plant Sciences, University of Missouri, Columbia, Missouri, USA.
authors.forename: William L
authors.initials: WL
authors.lastname: Franck
authors.affiliation: Department of Statistics, University of Missouri, Columbia, Missouri, USA.
authors.forename: Jing
authors.initials: J
authors.lastname: Qiu
authors.affiliation: Department of Biology, University of Texas, Arlington, Texas, USA.
authors.forename: Hae-In
authors.initials: HI
authors.lastname: Lee
authors.affiliation: Department of Biology, University of Texas, Arlington, Texas, USA Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, Republic of Korea wschang@uta.edu.
authors.forename: Woo-Suk
authors.initials: WS
authors.lastname: Chang
authors.affiliation: National Center for Soybean Biotechnology, Division of Plant Sciences, University of Missouri, Columbia, Missouri, USA Division of Biochemistry, University of Missouri, Columbia, Missouri, USA.
authors.forename: Gary
authors.initials: G
authors.lastname: Stacey
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D020369:Bradyrhizobium / Q000235:genetics* / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D018085:Regulon; D017422:Sequence Analysis, DNA
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; C083573:ntrC protein, Rhizobium; D009584:Nitrogen