title: Simultaneous detection of Entamoeba histolytica/dispar, Giardia duodenalis and cryptosporidia by immunochromatographic assay in stool samples from patients living in the Greater Cairo Region, Egypt.
abstract: Gastrointestinal infection due to intestinal parasites is an enormous health problem in developing countries and its reliable diagnosis is demanding. Therefore, this study aimed at evaluating a commercially available immunochromatographic assay (ICA) for the detection of cryptosporidia, Giardia duodenalis, and Entamoeba histolytica/dispar for its usefulness in the Greater Cairo Region, Egypt. Stool samples of 104 patients who presented between October 2012 and March 2013 with gastrointestinal symptoms or for the exclusion of parasites at Kasr-Al-Ainy University Medical School were examined by light microscopy of wet mounts and the triple ICA. Microscopy revealed in 20% of the patients [95% confidence interval (CI), 13.5-29.0%] parasites with Hymenolepis nana, E. histolytica/dispar and Blastocystis hominis being the most frequent ones, but was not able to detect G. duodenalis and cryptosporidia, whereas ICA was positive in 21% (95% CI, 14.3-30.0%) and detected E. histolytica/dispar in 12.5% (95% CI, 7.3-20.4%), cryptosporidia in 6.7% (95% CI, 3.1-13.5%) and G. duodenalis in 15.4% (95% CI, 9.6-23.6%) of the patients. Detection of one or more pathogens was associated with access to water retrieved from a well or pump (p = 0.01). Patients between 20 and 29 years of age (p = 0.08) and patients with symptoms of 5 days or longer (p = 0.07) tended to have a higher risk to be infected than patients of other age groups or with shorter-lasting symptoms. In conclusion, the ICA was easy to perform and timesaving. Importantly, it enabled the detection of cryptosporidia, which cannot be found microscopically in unstained smears, demonstrated a higher sensitivity for the detection of G. duodenalis than microscopy, and was more specific for distinguishing E. histolytica/dispar from apathogenic amoeba.
authors.affiliation: Institute of Tropical Medicine and International Health, Charité - Universitätsmedizin Berlin, Augustenburger Platz 1, 13353, Berlin, Germany, daggib@gmx.de.
authors.forename: Dagmar M
authors.initials: DM
authors.lastname: Banisch
authors.forename: Ayman
authors.initials: A
authors.lastname: El-Badry
authors.forename: Jorge V
authors.initials: JV
authors.lastname: Klinnert
authors.forename: Ralf
authors.initials: R
authors.lastname: Ignatius
authors.forename: Nadia
authors.initials: N
authors.lastname: El-Dib
mesh_terms: D000328:Adult; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D003457:Cryptosporidiosis / Q000175:diagnosis / Q000453:epidemiology / Q000469:parasitology*; D003458:Cryptosporidium / Q000737:chemistry / Q000302:isolation & purification*; D004534:Egypt / Q000453:epidemiology; D004748:Entamoeba histolytica / Q000737:chemistry / Q000302:isolation & purification*; D004749:Entamoebiasis / Q000175:diagnosis / Q000453:epidemiology / Q000469:parasitology*; D005243:Feces / Q000737:chemistry / Q000469:parasitology*; D005260:Female; D016829:Giardia lamblia / Q000737:chemistry / Q000302:isolation & purification*; D005873:Giardiasis / Q000175:diagnosis / Q000453:epidemiology / Q000469:parasitology*; D006801:Humans; D008297:Male; D011933:Reagent Kits, Diagnostic; D055815:Young Adult
chemical_list: D011933:Reagent Kits, Diagnostic