title: A FRET-based ratiometric redox probe for detecting oxidative stress by confocal microscopy, FLIM and flow cytometry.
abstract: Understanding the role of oxidative stress in disease requires real time monitoring of redox status within a cell. We report a FRET-based, ratiometric redox probe which can be applied to monitor cellular oxidative capacity using three different modalities – confocal microscopy, fluorescence lifetime imaging and flow cytometry.
authors.affiliation: School of Chemistry, The University of Sydney, New South Wales, 2006, Australia. elizabeth.new@sydney.edu.au.
authors.forename: Amandeep
authors.initials: A
authors.lastname: Kaur
authors.forename: Mohammad A
authors.initials: MA
authors.lastname: Haghighatbin
authors.forename: Conor F
authors.initials: CF
authors.lastname: Hogan
authors.forename: Elizabeth J
authors.initials: EJ
authors.lastname: New
mesh_terms: D005434:Flow Cytometry; D031541:Fluorescence Resonance Energy Transfer; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins / Q000138:chemical synthesis / Q000737:chemistry*; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D010084:Oxidation-Reduction; D018384:Oxidative Stress
chemical_list: D008164:Luminescent Proteins