title: Development of Multiple-Locus Variable-Number Tandem-Repeat Analysis for Molecular Subtyping of Campylobacter jejuni by Using Capillary Electrophoresis.
abstract: Campylobacter jejuni is a common cause of the frequently reported food-borne diseases in developed and developing nations. This study describes the development of multiple-locus variable-number tandem-repeat (VNTR) analysis (MLVA) using capillary electrophoresis as a novel typing method for microbial source tracking and epidemiological investigation of C. jejuni. Among 36 tandem repeat loci detected by the Tandem Repeat Finder program, 7 VNTR loci were selected and used for characterizing 60 isolates recovered from chicken meat samples from retail shops, samples from chicken meat processing factory, and stool samples. The discrimination ability of MLVA was compared with that of multilocus sequence typing (MLST). MLVA (diversity index of 0.97 with 31 MLVA types) provided slightly higher discrimination than MLST (diversity index of 0.95 with 25 MLST types). The overall concordance between MLVA and MLST was estimated at 63% by adjusted Rand coefficient. MLVA predicted MLST type better than MLST predicted MLVA type, as reflected by Wallace coefficient (Wallace coefficient for MLVA to MLST versus MLST to MLVA, 86% versus 51%). MLVA is a useful tool and can be used for effective monitoring of C. jejuni and investigation of epidemics caused by C. jejuni.
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Minato, Tokyo, Japan.
authors.forename: Punnida
authors.initials: P
authors.lastname: Techaruvichit
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Minato, Tokyo, Japan hajime@kaiyodai.ac.jp.
authors.forename: Hajime
authors.initials: H
authors.lastname: Takahashi
authors.affiliation: Research and Development Center of BETAGRO Group, Klong Luang, Pathumthani, Thailand.
authors.forename: Mongkol
authors.initials: M
authors.lastname: Vesaratchavest
authors.affiliation: Department of Food Technology, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Suwimon
authors.initials: S
authors.lastname: Keeratipibul
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Minato, Tokyo, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kuda
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Minato, Tokyo, Japan.
authors.forename: Bon
authors.initials: B
authors.lastname: Kimura
mesh_terms: D000818:Animals; D016123:Campylobacter jejuni / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D002645:Chickens; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D019075:Electrophoresis, Capillary / Q000379:methods*; D004783:Environmental Microbiology; D005243:Feces / Q000382:microbiology; D005511:Food Handling; D008460:Meat / Q000382:microbiology; D018598:Minisatellite Repeats; D017720:Molecular Epidemiology / Q000379:methods; D008969:Molecular Sequence Data; D058889:Molecular Typing / Q000379:methods*; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial