title: A novel protein quality control mechanism contributes to heat shock resistance of worldwide-distributed Pseudomonas aeruginosa clone C strains.
abstract: Pseudomonas aeruginosa is a highly successful nosocomial pathogen capable of causing a wide variety of infections with clone C strains most prevalent worldwide. In this study, we initially characterize a molecular mechanism of survival unique to clone C strains. We identified a P. aeruginosa clone C-specific genomic island (PACGI-1) that contains the highly expressed small heat shock protein sHsp20c, the founding member of a novel subclass of class B bacterial small heat shock proteins. sHsp20c and adjacent gene products are involved in resistance against heat shock. Heat stable sHsp20c is unconventionally expressed in stationary phase in a wide temperature range from 20 to 42°C. Purified sHsp20c has characteristic features of small heat shock protein class B as it is monodisperse, forms sphere-like 24-meric oligomers and exhibits significant chaperone activity. As the P. aeruginosa clone C population is significantly more heat shock resistant than genetically unrelated P. aeruginosa strains without sHsp20c, the horizontally acquired shsp20c operon might contribute to the survival of worldwide-distributed clone C strains.
authors.affiliation: Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, 171 77, Sweden.
authors.forename: Changhan
authors.initials: C
authors.lastname: Lee
authors.affiliation: Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, 171 77, Sweden.
authors.forename: Edvard
authors.initials: E
authors.lastname: Wigren
authors.affiliation: Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, 171 77, Sweden.
authors.forename: Janja
authors.initials: J
authors.lastname: Trček
authors.affiliation: Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, 171 77, Sweden.
authors.forename: Verena
authors.initials: V
authors.lastname: Peters
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, 305-701, Korea.
authors.forename: Jihong
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, 171 77, Sweden.
authors.forename: Muhammad Sharif
authors.initials: MS
authors.lastname: Hasni
authors.affiliation: The Helmholtz Center for Infection Research, Braunschweig, 38124, Germany.
authors.forename: Manfred
authors.initials: M
authors.lastname: Nimtz
authors.affiliation: Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, 171 77, Sweden.
authors.forename: Ylva
authors.initials: Y
authors.lastname: Lindqvist
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, 305-701, Korea.
authors.forename: Chankyu
authors.initials: C
authors.lastname: Park
authors.affiliation: Institute for Biophysical Chemistry, Hannover Medical School, Hannover, 30625, Germany.
authors.forename: Ute
authors.initials: U
authors.lastname: Curth
authors.affiliation: The Helmholtz Center for Infection Research, Braunschweig, 38124, Germany.
authors.forename: Heinrich
authors.initials: H
authors.lastname: Lünsdorf
authors.affiliation: Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, 171 77, Sweden.
authors.forename: Ute
authors.initials: U
authors.lastname: Römling
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003428:Cross Infection / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D044404:Genomic Islands / Q000235:genetics*; D006360:Heat-Shock Proteins / Q000235:genetics*; D018869:Heat-Shock Response / Q000502:physiology*; D006358:Hot Temperature; D008969:Molecular Sequence Data; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D006360:Heat-Shock Proteins