title: Hygienic treatment and energy recovery of dead animals by high solid co-digestion with vinasse under mesophilic condition: feasibility study.
abstract: In the present study, the feasibility of hygienic treatment and energy recovery of dead animals (pork as an experimental alternative) by high solid co-digestion with vinasse under mesophilic condition was investigated. A lab-scale digester was operated for 125 days with SRT of 20 days. The volatile solid (VS) reduction, biogas yield, and CH4 content finally achieved stability after two obvious fluctuations, and were 55±1%, 0.40±0.02 m(3)/kg VS feed and 68%, respectively. Meanwhile, the shifts of microbial communities of the samples taken on typical days with different performance (day 40 (stable), day 65 (deteriorated), day 75 (in recovery), day 90 (recovered) and day 110 (stable)) were monitored by the application of pyrosequencing technology and the bioinformatical analysis with QIIME 1.8. In consequence, the details of changes in bacterial and archaeal communities elaborately explained the intrinsic reasons for the deterioration and recovery of the digester, and provided the proofs for the feasibility of hygienic treatment and energy recovery of dead animals by high solid co-digestion with vinasse under mesophilic condition.
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University,1239 Siping Road,Shanghai 200092, PR China.
authors.forename: Xiaohu
authors.initials: X
authors.lastname: Dai
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University,1239 Siping Road,Shanghai 200092, PR China.
authors.forename: Sisi
authors.initials: S
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University,1239 Siping Road,Shanghai 200092, PR China.
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Xue
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University,1239 Siping Road,Shanghai 200092, PR China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Dai
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University,1239 Siping Road,Shanghai 200092, PR China. Electronic address: lining@tongji.edu.cn.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University,1239 Siping Road,Shanghai 200092, PR China; Graduate School of Animal Sciences, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 0808555, Japan.
authors.forename: Junichi
authors.initials: J
authors.lastname: Takahashi
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University,1239 Siping Road,Shanghai 200092, PR China.
authors.forename: Wentao
authors.initials: W
authors.lastname: Zhao
mesh_terms: D000693:Anaerobiosis; D000818:Animals; D001105:Archaea / Q000378:metabolism; D056804:Biofuels; D015415:Biomarkers / Q000378:metabolism; D019149:Bioreactors; D019295:Computational Biology; D004247:DNA / Q000032:analysis; D017753:Ecosystem; D005240:Feasibility Studies; D006863:Hydrogen-Ion Concentration; D008697:Methane / Q000032:analysis* / Q000737:chemistry; D000069466:Red Meat; D012037:Refuse Disposal; D017422:Sequence Analysis, DNA
chemical_list: D056804:Biofuels; D015415:Biomarkers; D004247:DNA; D008697:Methane
keywords: Bioinformatical analysis; Co-digestion; Feasibility; High solid; Pyrosequencing