title: Utilization of recombinant Trichoderma reesei expressing Aspergillus aculeatus β-glucosidase I (JN11) for a more economical production of ethanol from lignocellulosic biomass.
abstract: The capacity of Trichoderma reesei cellulase to degrade lignocellulosic biomass has been enhanced by the construction of a recombinant T. reesei strain expressing Aspergillus aculeatus β-glucosidase I. We have confirmed highly efficient ethanol production from converge-milled Japanese cedar by recombinant T. reesei expressing A. aculeatus β-glucosidase I (JN11). We investigated the ethanol productivity of JN11 and compared it with the cocktail enzyme T. reesei PC-3-7 with reinforced cellobiase activity by the commercial Novozyme 188. Results showed that the ethanol production efficiency under enzymatic hydrolysis of JN11 was comparable to the cocktail enzyme both on simultaneous saccharification and fermentation (SSF) or separate hydrolysis and fermentation (SHF) processes. Moreover, the cocktail enzyme required more protein loading for attaining similar levels of ethanol conversion as JN11. We propose that JN11 is an intrinsically economical enzyme that can eliminate the supplementation of BGL for PC-3-7, thereby reducing the cost of industrial ethanol production from lignocellulosic biomass.
authors.affiliation: Department of Bioengineering, Nagaoka University of Technology, Kamitomioka-cho, 1603-1, Nagaoka, Niigata 940-2188, Japan.
authors.forename: Treesukon
authors.initials: T
authors.lastname: Treebupachatsakul
authors.affiliation: Department of Bioengineering, Nagaoka University of Technology, Kamitomioka-cho, 1603-1, Nagaoka, Niigata 940-2188, Japan.
authors.forename: Koki
authors.initials: K
authors.lastname: Shioya
authors.affiliation: Department of Bioengineering, Nagaoka University of Technology, Kamitomioka-cho, 1603-1, Nagaoka, Niigata 940-2188, Japan.
authors.forename: Hikaru
authors.initials: H
authors.lastname: Nakazawa
authors.affiliation: Graduate School of Agriculture and Biological Sciences and Research Institute for Advanced Science and Technology, Osaka Prefecture University, 1-1 Gakuen-cho, Sakai, Osaka 599-8531, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kawaguchi
authors.affiliation: Department of Bioengineering, Nagaoka University of Technology, Kamitomioka-cho, 1603-1, Nagaoka, Niigata 940-2188, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Morikawa
authors.affiliation: Department of Bioengineering, Nagaoka University of Technology, Kamitomioka-cho, 1603-1, Nagaoka, Niigata 940-2188, Japan.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Shida
authors.affiliation: Department of Bioengineering, Nagaoka University of Technology, Kamitomioka-cho, 1603-1, Nagaoka, Niigata 940-2188, Japan. Electronic address: owataru@vos.nagaokaut.ac.jp.
authors.forename: Wataru
authors.initials: W
authors.lastname: Ogasawara
authors.affiliation: Department of Bioengineering, Nagaoka University of Technology, Kamitomioka-cho, 1603-1, Nagaoka, Niigata 940-2188, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Okada
mesh_terms: D001230:Aspergillus / Q000201:enzymology* / Q000235:genetics; D018533:Biomass; D002480:Cellulase / Q000378:metabolism; D032485:Cryptomeria / Q000737:chemistry; D000431:Ethanol / Q000032:analysis / Q000191:economics / Q000378:metabolism*; D005285:Fermentation; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism*; D014242:Trichoderma / Q000235:genetics* / Q000378:metabolism*; D001617:beta-Glucosidase / Q000191:economics / Q000235:genetics* / Q000378:metabolism*
chemical_list: C036909:lignocellulose; D000431:Ethanol; D008031:Lignin; C427304:Novozym 188; D001617:beta-Glucosidase; D002480:Cellulase
keywords: Aspergillus aculeatus BGL I; Ethanol; Separate hydrolysis and fermentation; Simultaneous saccharification and fermentation; Trichoderma reesei