title: TaqMan qPCR for detection and quantification of mitochondrial DNA from toxic pufferfish species.
abstract: Outbreaks of pufferfish food poisoning have been reported worldwide, most were from unsuitable food preparation and adulteration. In order to rapidly detect pufferfish adulterant in processed foods, we developed and proposed the use of a TaqMan probe-based quantitative PCR. The designed detection oligos targeted a unique region in 16S rDNA of toxic marine pufferfish in Tetraodontidae Family and gave a positive signal at ≥1.75 pg of genomic DNA. Non-target DNA samples from other fish, chicken, and beef were negative.
authors.affiliation: Forensic Science Graduate Program, Faculty of Science, Mahidol University, Bangkok, Thailand.
authors.forename: Tassanee
authors.initials: T
authors.lastname: Luekasemsuk
authors.affiliation: Department of Plant Science, Faculty of Science, Mahidol University, Bangkok, Thailand.
authors.forename: Nathinee
authors.initials: N
authors.lastname: Panvisavas
authors.affiliation: Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand. Electronic address: soraya.cha@mahidol.ac.th.
authors.forename: Soraya
authors.initials: S
authors.lastname: Chaturongakul
mesh_terms: D000818:Animals; D002417:Cattle; D004272:DNA, Mitochondrial / Q000032:analysis*; D005396:Fish Products / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D008387:Marine Toxins / Q000032:analysis*; D011202:Poultry Products / Q000032:analysis*; D060888:Real-Time Polymerase Chain Reaction; D000069466:Red Meat / Q000032:analysis*; D013045:Species Specificity; D023721:Tetraodontiformes; D013785:Thailand
chemical_list: D004272:DNA, Mitochondrial; D008387:Marine Toxins
keywords: Pufferfish; Quantitative real-time detection; TaqMan PCR; Tetraodontidae