title: Five pectinase gene expressions highly responding to heat stress in rice floral organs revealed by RNA-seq analysis.
abstract: Heat stress hurts rice, and floral organs are mostly sensitive to heat stress. We aimed to unravel molecular responses to heat stress in rice floral organs using Illumina/Solexa sequencing technology for addressing the increasing concern of globle warming. At meiophase of the pollen mother cell (pulvinus flat), the plants were stressed for 3 d at 38 C, and RNA was extracted from the stressed pistil and stamen for RNA-Seq sequencing to build the heat stress transcriptom library. A total of 7178 defferentially expressed genes (DEGs) between the normal and heat stress libraries were significant, 61% up-regulated and 39% down-regulated. The 7178 DEGs were significantly classified to 34 gene ontology (GO) categories, and 11 of the GO categories were significantly enriched. The GO:0016787 for hydrolase activity of molecular function was mostly enriched with the least probability, and included 11 DEGs named Hy1 - Hy11. Expression levels of five DEGs, Hy4 - Hy6 and Hy9 - Hy10 for starch and sucrose metablism via pectinase, increased 12 - 14 times in response to the heat stress. Further investigation of the five DEGs for pectin metabolism and association with reported heat responsive genes may help develop a molecular strategy to remedy heat damage in rice.
authors.affiliation: Center of Cooperative Innovation for Crop, Agronomy College, Anhui Agricultural University, Hefei 230036, Anhui, China; Jiangsu Collaboraive Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.
authors.forename: Liquan
authors.initials: L
authors.lastname: Wu
authors.affiliation: Center of Cooperative Innovation for Crop, Agronomy College, Anhui Agricultural University, Hefei 230036, Anhui, China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Taohua
authors.affiliation: Center of Cooperative Innovation for Crop, Agronomy College, Anhui Agricultural University, Hefei 230036, Anhui, China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Gui
authors.affiliation: Center of Cooperative Innovation for Crop, Agronomy College, Anhui Agricultural University, Hefei 230036, Anhui, China.
authors.forename: Lisen
authors.initials: L
authors.lastname: Xu
authors.affiliation: Center of Cooperative Innovation for Crop, Agronomy College, Anhui Agricultural University, Hefei 230036, Anhui, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.affiliation: Jiangsu Collaboraive Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China. Electronic address: dingyf@njau.edu.cn.
authors.forename: YanFeng
authors.initials: Y
authors.lastname: Ding
mesh_terms: D035264:Flowers / Q000235:genetics / Q000502:physiology; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018869:Heat-Shock Response; D006358:Hot Temperature; D012275:Oryza / Q000235:genetics* / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics*; D011096:Polygalacturonase / Q000235:genetics*
chemical_list: D010940:Plant Proteins; D011096:Polygalacturonase
keywords: High temperature damage; Pectinase; RNA-seq; Rice; Transcriptome