title: Development of a LC-MS-MS Method for Quantification of Valtrate and Its Application to Pharmacokinetic Study.
abstract: A simple and selective liquid chromatography-tandem mass spectrometric method for determination of valtrate in rat plasma was developed in this study. Chromatographic separation was achieved on a Thermo BDS HYPERSIL C18 column using acetonitrile-water-formic acid (75 : 25 : 0.1, v/v/v) as mobile phase in an isocratic mode of elution at a flow rate of 300 µL/min. MS-MS detection was performed in a positive ion electrospray ionization mode with the ion transitions 445.2 → 219.2 for valtrate and 355.2 → 135.1 for internal standard (sesamin). The developed method exhibited a linear dynamic range over 5.65-1695 ng/mL for valtrate in rat plasma. The overall extraction recovery of valtrate from plasma was 86.13-88.32%. The intra- and inter-day accuracy and precision were within the pre-defined limits of ≤15% at all concentrations. The method was successfully applied to pharmacokinetic studies of valtrate in rats.
authors.affiliation: Emergency Department, The First Hospital of Jilin University, Changchun 130021, China.
authors.forename: Lichao
authors.initials: L
authors.lastname: Sun
authors.affiliation: Neurosurgery Department, The First Hospital of Jilin University, Changchun 130021, China.
authors.forename: Jiqing
authors.initials: J
authors.lastname: Qiu
authors.affiliation: Neurosurgery Department, The First Hospital of Jilin University, Changchun 130021, China.
authors.forename: Guangming
authors.initials: G
authors.lastname: Wang
authors.affiliation: Neurology Department, The First Hospital of Jilin University, Changchun 130021, China linwhdaqd@hotmail.com.
authors.forename: Weihong
authors.initials: W
authors.lastname: Lin
authors.affiliation: Emergency Department, The First Hospital of Jilin University, Changchun 130021, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D039823:Iridoids / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D016014:Linear Models; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D039823:Iridoids; C030127:valtrate