title: Changes in the functional characteristics of tumor and normal cells after treatment with extracts of white dead-nettle.
abstract: Lamium albumL. is a perennial herb widely used in folk medicine. It possesses a wide spectrum of therapeutic activities (anti-inflammatory, astringent, antiseptic, antibiotic, antispasmodic, antioxidant and anti-proliferative). Preservation of medicinal plant could be done byin vitropropagation to avoid depletion from their natural habitat. It is important to know whether extracts fromL. albumplants grownin vitropossess similar properties as extracts from plants grownin vivo. For these reasons, it is important to examine changes in the composition of secondary metabolites duringin vitrocultivation of the plant and how they affect the biological activity. We used A549 human cancer cell line and normal kidney epithelial cells MDCKII (Madin-Darby canine kidney cells II) as controls in assessing the anti-cancer effect of plant extracts. To elucidate changes in some key functional characteristics, adhesion test, MTT (3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyl tetrazolium bromide), transepithelial resistance (TER), immunofluorescence staining and trypan blue exclusion test were performed. Methanol and chloroform extracts ofin vivoandin vitropropagated plants affected differently cancerous and non-cancerous cells. The most pronounced differences were observed in the morphological analysis and in the cell adhesive properties. We also detected suppressed epithelial transmembrane electrical resistance of MDCK II cells, by treatment with plant extracts, compared to non-treated MDCK II cells. A549 cells did not polarize under the same conditions. Altered organization of actin filaments in both cell types were noticed suggesting that extracts fromL. albumL. change TER and actin filaments, and somehow may block cell mechanisms, leading to the polarization of MDCK II cells.
authors.affiliation: Department of Cytology, Histology and Embryology, Faculty of Biology, Sofia University 'St. Kl. Ohridski' , Sofia , Bulgaria.
authors.forename: Ralitsa
authors.initials: R
authors.lastname: Veleva
authors.affiliation: Department of Plant Physiology, Faculty of Biology, Sofia University 'St. Kl. Ohridski' , Sofia , Bulgaria.
authors.forename: Bela
authors.initials: B
authors.lastname: Petkova
authors.affiliation: Department of Cytology, Histology and Embryology, Faculty of Biology, Sofia University 'St. Kl. Ohridski' , Sofia , Bulgaria.
authors.forename: Veselina
authors.initials: V
authors.lastname: Moskova-Doumanova
authors.affiliation: Department of Biochemistry, Faculty of Biology, Sofia University 'St. Kl. Ohridski' , Sofia , Bulgaria.
authors.forename: Jordan
authors.initials: J
authors.lastname: Doumanov
authors.affiliation: Department of Plant Physiology, Faculty of Biology, Sofia University 'St. Kl. Ohridski' , Sofia , Bulgaria.
authors.forename: Milena
authors.initials: M
authors.lastname: Dimitrova
authors.affiliation: Department of Cytology, Histology and Embryology, Faculty of Biology, Sofia University 'St. Kl. Ohridski' , Sofia , Bulgaria.
authors.forename: Petya
authors.initials: P
authors.lastname: Koleva
authors.affiliation: Department of Biochemistry, Faculty of Biology, Sofia University 'St. Kl. Ohridski' , Sofia , Bulgaria.
authors.forename: Kirilka
authors.initials: K
authors.lastname: Mladenova
authors.affiliation: Department of Biochemistry, Faculty of Biology, Sofia University 'St. Kl. Ohridski' , Sofia , Bulgaria.
authors.forename: Svetla
authors.initials: S
authors.lastname: Petrova
authors.affiliation: Department of Plant Physiology, Faculty of Biology, Sofia University 'St. Kl. Ohridski' , Sofia , Bulgaria.
authors.forename: Zhenya
authors.initials: Z
authors.lastname: Yordanova
authors.affiliation: Department of Plant Physiology, Faculty of Biology, Sofia University 'St. Kl. Ohridski' , Sofia , Bulgaria.
authors.forename: Veneta
authors.initials: V
authors.lastname: Kapchina-Toteva
authors.affiliation: Department of Cytology, Histology and Embryology, Faculty of Biology, Sofia University 'St. Kl. Ohridski' , Sofia , Bulgaria.
authors.forename: Tanya
authors.initials: T
authors.lastname: Topouzova-Hristova
keywords: A549; Lamium album L.; MDCKII; actin cytoskeleton; cytotoxicity; plant extracts