title: Dexamethasone-Conjugated Polyamidoamine Dendrimer for Delivery of the Heme Oxygenase-1 Gene into the Ischemic Brain.
abstract: Heme oxygenase-1 (HO-1) has anti-apoptotic and anti-inflammatory effects. In this study, the HO-1 gene was delivered into the brain using dexamethasone-conjugated polyamidoamine generation 2 (PAMAM G2-Dexa) for the treatment of ischemic stroke. PAMAM G2-Dexa formed stable complexes with plasmid DNA (pDNA). The pDNA delivery efficiency of PAMAM G2-Dexa was higher than that of polyethylenimine (PEI25k, 25 kDa), dexamethasone-conjugated PEI (PEI-Dexa), and PAMAM G2 in Neuro2A cells. Therapeutic effect of PAMAM G2-Dexa/pHO-1 complexes was evaluated in a stroke animal model. PAMAM G2-Dexa delivered pHO-1 more efficiently into the ischemic brain than PEI25k and PEI-Dexa with higher therapeutic effect. Therefore, PAMAM G2-Dexa/pHO-1 complexes may be useful for ischemic stroke gene therapy.
authors.affiliation: BK21 Plus Future Biopharmaceutical Human Resources Training and Research Team, Department of Bioengineering, College of Engineering, Hanyang University, Seoul 133-791, Korea.
authors.forename: Pureum
authors.initials: P
authors.lastname: Jeon
authors.affiliation: BK21 Plus Future Biopharmaceutical Human Resources Training and Research Team, Department of Bioengineering, College of Engineering, Hanyang University, Seoul 133-791, Korea.
authors.forename: Manbok
authors.initials: M
authors.lastname: Choi
authors.affiliation: BK21 Plus Future Biopharmaceutical Human Resources Training and Research Team, Department of Bioengineering, College of Engineering, Hanyang University, Seoul 133-791, Korea.
authors.forename: Jungju
authors.initials: J
authors.lastname: Oh
authors.affiliation: BK21 Plus Future Biopharmaceutical Human Resources Training and Research Team, Department of Bioengineering, College of Engineering, Hanyang University, Seoul 133-791, Korea. minhyung@hanyang.ac.kr.
authors.forename: Minhyung
authors.initials: M
authors.lastname: Lee
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects; D002545:Brain Ischemia / Q000628:therapy*; D050091:Dendrimers; D003907:Dexamethasone; D004195:Disease Models, Animal; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D051547:Heme Oxygenase-1 / Q000235:genetics*; D051379:Mice; D010957:Plasmids; D011073:Polyamines; D051381:Rats
chemical_list: D050091:Dendrimers; C531249:Poly(amidoamine); D011073:Polyamines; D003907:Dexamethasone; D051547:Heme Oxygenase-1
keywords: dexamethasone; gene therapy; heme oxygenase-1; polyamidoamine; stroke