title: MiR-451 inhibits proliferation of esophageal carcinoma cell line EC9706 by targeting CDKN2D and MAP3K1.
abstract: OBJECTIVE
  To investigate the underlying molecular mechanisms of miR-451 to inhibit proliferation of esophageal carcinoma cell line EC9706.
  
  
  METHODS
  Assays for cell growth, apoptosis and invasion were used to evaluate the effects of miR-451 expression on EC cells. Luciferase reporter and Western blot assays were used to test whether cyclin-dependent kinase inhibitor 2D (CDKN2D) and MAP3K1 act as major targets of miR-451.
  
  
  RESULTS
  The results showed that CDKN2D and MAP3K1 are direct targets of miR-451. CDKN2D and MAP3K1 overexpression reversed the effect of miR-451. MiR-451 inhibited the proliferation of EC9706 by targeting CDKN2D and MAP3K1.
  
  
  CONCLUSIONS
  These findings suggest that miR-451 might be a novel prognostic biomarker and a potential target for the treatment of esophageal squamous cell carcinoma in the future.
authors.affiliation: Wen-Qiao Zang, Xuan Yang, Yuan-Yuan Wang, Yu-Wen Du, Xiao-Nan Chen, Min Li, Guo-Qiang Zhao, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, Henan Province, China.
authors.forename: Wen-Qiao
authors.initials: WQ
authors.lastname: Zang
authors.affiliation: Wen-Qiao Zang, Xuan Yang, Yuan-Yuan Wang, Yu-Wen Du, Xiao-Nan Chen, Min Li, Guo-Qiang Zhao, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, Henan Province, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Yang
authors.affiliation: Wen-Qiao Zang, Xuan Yang, Yuan-Yuan Wang, Yu-Wen Du, Xiao-Nan Chen, Min Li, Guo-Qiang Zhao, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, Henan Province, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.affiliation: Wen-Qiao Zang, Xuan Yang, Yuan-Yuan Wang, Yu-Wen Du, Xiao-Nan Chen, Min Li, Guo-Qiang Zhao, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, Henan Province, China.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Wang
authors.affiliation: Wen-Qiao Zang, Xuan Yang, Yuan-Yuan Wang, Yu-Wen Du, Xiao-Nan Chen, Min Li, Guo-Qiang Zhao, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, Henan Province, China.
authors.forename: Yu-Wen
authors.initials: YW
authors.lastname: Du
authors.affiliation: Wen-Qiao Zang, Xuan Yang, Yuan-Yuan Wang, Yu-Wen Du, Xiao-Nan Chen, Min Li, Guo-Qiang Zhao, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, Henan Province, China.
authors.forename: Xiao-Nan
authors.initials: XN
authors.lastname: Chen
authors.affiliation: Wen-Qiao Zang, Xuan Yang, Yuan-Yuan Wang, Yu-Wen Du, Xiao-Nan Chen, Min Li, Guo-Qiang Zhao, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, Henan Province, China.
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.affiliation: Wen-Qiao Zang, Xuan Yang, Yuan-Yuan Wang, Yu-Wen Du, Xiao-Nan Chen, Min Li, Guo-Qiang Zhao, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, Henan Province, China.
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Zhao
mesh_terms: D002277:Carcinoma / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D050764:Cyclin-Dependent Kinase Inhibitor p19 / Q000235:genetics / Q000378:metabolism*; D004938:Esophageal Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D000077277:Esophageal Squamous Cell Carcinoma; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D048728:MAP Kinase Kinase Kinase 1 / Q000235:genetics / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D014162:Transfection
chemical_list: C493974:CDKN2D protein, human; D050764:Cyclin-Dependent Kinase Inhibitor p19; C531201:MIRN451 microRNA, human; D035683:MicroRNAs; D048728:MAP Kinase Kinase Kinase 1; C482576:MAP3K1 protein, human
keywords: Cyclin-dependent kinase inhibitor 2D; Esophageal squamous cell carcinoma; MAP3K1; MiR-451; Proliferation