title: Statistical design and optimization of single cell oil production from sugarcane bagasse hydrolysate by an oleaginous yeast Rhodotorula sp. IIP-33 using response surface methodology.
abstract: Single cell oil production from sugarcane bagasse hydrolysate by oleaginous yeast Rhodotorula sp. IIP-33 was analyzed using a two stage statistical design approach based on Response Surface Methodology. Variables like pentose sugar, (NH4)2SO4, KH2PO4, yeast extract, pH and temperature were found to influence lipid production significantly. Under optimized condition in a shake flask, yield of lipid was 2.1199 g with fat coefficient of 7.09 which also resembled ~99% similarity to model predicted lipid production. In this paper we are presenting optimized results for production of non polar lipid which could be later deoxygenated into hydrocarbon. A qualitative analyses of selective lipid samples yielded a varying distribution of free acid ranging from C6 to C18, majoring C16:0, C18:0 and C18:1 under different fermentation conditions.
authors.affiliation: Biofuels Division, CSIR-Indian Institute of Petroleum, Mohkampur, Dehradun, 248 005 Uttarakhand India.
authors.forename: Sheetal
authors.initials: S
authors.lastname: Bandhu
authors.affiliation: Biofuels Division, CSIR-Indian Institute of Petroleum, Mohkampur, Dehradun, 248 005 Uttarakhand India.
authors.forename: Diptarka
authors.initials: D
authors.lastname: Dasgupta
authors.affiliation: Biofuels Division, CSIR-Indian Institute of Petroleum, Mohkampur, Dehradun, 248 005 Uttarakhand India.
authors.forename: Jawed
authors.initials: J
authors.lastname: Akhter
authors.affiliation: Analytical Sciences Division, CSIR-Indian Institute of Petroleum, Mohkampur, Dehradun, 248 005 Uttarakhand India.
authors.forename: Pankaj
authors.initials: P
authors.lastname: Kanaujia
authors.affiliation: Biofuels Division, CSIR-Indian Institute of Petroleum, Mohkampur, Dehradun, 248 005 Uttarakhand India.
authors.forename: Sunil K
authors.initials: SK
authors.lastname: Suman
authors.affiliation: Biofuels Division, CSIR-Indian Institute of Petroleum, Mohkampur, Dehradun, 248 005 Uttarakhand India.
authors.forename: Deepti
authors.initials: D
authors.lastname: Agrawal
authors.affiliation: Biofuels Division, CSIR-Indian Institute of Petroleum, Mohkampur, Dehradun, 248 005 Uttarakhand India.
authors.forename: Savita
authors.initials: S
authors.lastname: Kaul
authors.affiliation: Biofuels Division, CSIR-Indian Institute of Petroleum, Mohkampur, Dehradun, 248 005 Uttarakhand India.
authors.forename: Dilip K
authors.initials: DK
authors.lastname: Adhikari
authors.affiliation: Biofuels Division, CSIR-Indian Institute of Petroleum, Mohkampur, Dehradun, 248 005 Uttarakhand India.
authors.forename: Debashish
authors.initials: D
authors.lastname: Ghosh
keywords: Lignocellulosic biomass; Pentose sugar; Response surface methodology; Rhodotorula sp; Yeast lipid