title: Hairy Cell Leukemia with Systemic Lymphadenopathy: Detection of BRAF Mutations in Both Lymph Node and Peripheral Blood Specimens.
abstract: A 47-year-old woman with pancytopenia, excessive systemic lymphadenopathy and splenomegaly was referred to our hospital. The peripheral blood (PB) smear findings indicated neutropenia with lymphoid cells exhibiting hairy projections, while the histological findings of the cervical lymph node (LN) suggested hairy cell leukemia (HCL). In addition, the BRAF V600E mutation was detected, and the immunoglobulin gene rearrangement patterns were identical in both the cervical LN and PB specimens. Based on these findings, we diagnosed the patient with systemic lymphadenopathy due to HCL. This is the first report of a BRAF mutation detected in both the PB and LN at the onset of HCL.
authors.affiliation: Department of Hematology and Oncology, Kurashiki Central Hospital, Japan.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Okada
authors.forename: Akane
authors.initials: A
authors.lastname: Kunitomi
authors.forename: Kazuya
authors.initials: K
authors.lastname: Sakai
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Muranushi
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Okamoto
authors.forename: Taku
authors.initials: T
authors.lastname: Tsukamoto
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sugiura
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Matsui
authors.forename: Tomoyasu
authors.initials: T
authors.lastname: Jo
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Ueda
authors.forename: Tatsuhito
authors.initials: T
authors.lastname: Onishi
authors.forename: Masaru
authors.initials: M
authors.lastname: Ide
authors.forename: Shinya
authors.initials: S
authors.lastname: Kimura
authors.forename: Kenji
authors.initials: K
authors.lastname: Notohara
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Ueda
mesh_terms: D005260:Female; D006801:Humans; D007943:Leukemia, Hairy Cell / Q000175:diagnosis* / Q000235:genetics*; D008198:Lymph Nodes / Q000473:pathology*; D008206:Lymphatic Diseases / Q000473:pathology*; D010198:Pancytopenia; D020641:Polymorphism, Single Nucleotide; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics*; D013163:Splenomegaly / Q000235:genetics
chemical_list: C482119:BRAF protein, human; D048493:Proto-Oncogene Proteins B-raf