title: Crosslinking effect of dialdehyde starch (DAS) on decellularized porcine aortas for tissue engineering.
abstract: Biological tissue-derived biomaterials must be chemically modified to avoid immediate degradation and immune response before being implanted in human body to replace malfunctioning organs. DAS with active aldehyde groups was employed to replace glutaraldehyde (GA), a most common synthetic crosslinking reagent in clinical practice, to fix bioprostheses for lower cytotoxicity. The aim of this research was to evaluate fixation effect of DAS. The tensile strength, crosslinking stability, cytotoxicity especially the anti-calcification capability of DAS-fixed tissues were investigated. The tensile strength and resistance to enzymatic degradation of samples were increased after DAS fixation, the values maintained stably in D-Hanks solution for several days. Meanwhile, ultrastructure of samples preserved well and the anti-calcification capability of samples were improved, the amount of positive staining points in the whole visual field of 15% DAS-fixed samples was only 0.576 times to GA-fixed ones. Moreover, both unreacted DAS and its hydrolytic products were nontoxic in cytotoxicity study. The results demonstrated DAS might be an effective crosslinking reagent to fix biological tissue-derived biomaterials in tissue engineering.
authors.affiliation: College of Polymer Science and Engineering, Sichuan University, Chengdu 610065, PR China.
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu 610065, PR China.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Gu
authors.affiliation: College of Polymer Science and Engineering, Sichuan University, Chengdu 610065, PR China.
authors.forename: Huanhuan
authors.initials: H
authors.lastname: Qin
authors.affiliation: Department of Oncology, the 452 Hospital of Chinese PLA, Chengdu, Sichuan Province 610021, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: College of Polymer Science and Engineering, Sichuan University, Chengdu 610065, PR China.
authors.forename: Xu
authors.initials: X
authors.lastname: Yang
authors.affiliation: College of Polymer Science and Engineering, Sichuan University, Chengdu 610065, PR China. Electronic address: yuxixun@163.com.
authors.forename: Xixun
authors.initials: X
authors.lastname: Yu
mesh_terms: D000818:Animals; D001011:Aorta / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry* / Q000627:therapeutic use; D001705:Bioprosthesis / Q000009:adverse effects; D002113:Calcification, Physiologic / Q000187:drug effects; D005976:Glutaral / Q000737:chemistry / Q000627:therapeutic use*; D006801:Humans; D013213:Starch / Q000031:analogs & derivatives* / Q000737:chemistry / Q000627:therapeutic use; D013552:Swine; D013718:Tensile Strength; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials; D013213:Starch; C012880:dialdehyde starch; D005976:Glutaral
keywords: Anti-calcification; Bioprosthesis; Crosslinking characteristics; Cytotoxicity; Dialdehyde starch