title: Direct degradation of cellulosic biomass to bio-hydrogen from a newly isolated strain Clostridium sartagoforme FZ11.
abstract: A mesophilic hydrogen-producing strain, Clostridium sartagoforme FZ11, had been newly isolated from cow dung compost acclimated using microcrystalline cellulose (MCC) for at least 30 rounds in an anaerobic bioreactor, and identified by the 16S rDNA gene sequencing, which could directly utilized various carbon sources, especially cellulosic biomass, to produce hydrogen. The maximum hydrogen yields from MCC (10 g/l) and carboxymethyl cellulose (CMC, 10 g/l) were 77.2 and 64.6 ml/g, separately. Furthermore, some key parameters of affecting hydrogen production from raw corn stalk were also optimized. The maximal hydrogen yield and substrate degradation rate from raw corn stalk were 87.2 ml/g and 41.2% under the optimized conditions with substrate concentration of 15 g/l, phosphate buffer of 0.15 M, urea of 6 g/l and initial pH of 6.47 at 35 °C. The result showed that the strain FZ11 would be an ideal candidate to directly convert cellulosic biomass into bio-hydrogen without substrate pretreatment.
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450052, PR China.
authors.forename: Jing-Nan
authors.initials: JN
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450052, PR China.
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Li
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450052, PR China; College of Chemistry and Environment, Henan Institute of Education, Zhengzhou 450046, PR China.
authors.forename: Hui-Qin
authors.initials: HQ
authors.lastname: Zheng
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450052, PR China. Electronic address: yt.fan@zzu.edu.cn.
authors.forename: Yao-Ting
authors.initials: YT
authors.lastname: Fan
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450052, PR China.
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Hou
mesh_terms: D000818:Animals; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D002244:Carbon / Q000378:metabolism; D002417:Cattle; D002482:Cellulose / Q000378:metabolism*; D003013:Clostridium / Q000378:metabolism*; D005285:Fermentation / Q000502:physiology; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D013696:Temperature; D003313:Zea mays / Q000378:metabolism
chemical_list: D002244:Carbon; D006859:Hydrogen; D002482:Cellulose; C109691:microcrystalline cellulose
keywords: Bio-degradation; Cellulosic biomass; Clostridium sartagoforme FZ11; Fermentative hydrogen production; Isolation and identification