title: Expression profile of microRNAs in gastrointestinal stromal tumors revealed by high throughput quantitative RT-PCR microarray.
abstract: OBJECTIVE
  To investigate the microRNA (miRNA) expression profile in gastrointestinal stromal tumor (GIST) tissues that could serve as a novel diagnostic biomarker for GIST detection.
  
  
  METHODS
  We performed a quantitative real-time quantitative reverse transcriptase polymerase chain reaction assay to analyze the expression of 1888 miRNAs in a sample set that included 54 GIST tissue samples.
  
  
  RESULTS
  We found that dysregulation of several miRNAs may be related to the malignant potential of GISTs. Six of these miRNAs, hsa-let-7c, miR-218, miR-488#, miR-4683, miR-34c-5p and miR-4773, were selected as the final list of biomarkers to separate the malignant GISTs (M group) from the benign GISTs (B group). In addition, MiR-29b-2#, hsa-let-7c, miR-891b, miR-218, miR-204, miR-204-3p, miR-628-5p, miR-744, miR-29c#, miR-625 and miR-196a were used to distinguish between the borderline (BO group) and M groups. There were 11 common miRNAs selected to separate the benign and borderline (BB) group from the M group, including hsa-let-7c, miR-218, miR-628-5p, miR-204-3p, miR-204, miR-891b, miR-488#, miR-145, miR-891a, miR-34c-5p and miR-196a.
  
  
  CONCLUSIONS
  The identified miRNAs appear to be novel biomarkers to distinguish malignant from benign GISTs, which may be helpful to understand the mechanisms of GIST oncogenesis and progression, and to further elucidate the characteristics of GIST subtypes.
authors.affiliation: Han-Xing Tong, Yong Zhang, Jiong-Yuan Wang, Quan Jiang, Jun-Yi He, Ye-Bo Shao, Jun Zhu, Wei-Qi Lu, Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Han-Xing
authors.initials: HX
authors.lastname: Tong
authors.affiliation: Han-Xing Tong, Yong Zhang, Jiong-Yuan Wang, Quan Jiang, Jun-Yi He, Ye-Bo Shao, Jun Zhu, Wei-Qi Lu, Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Yu-Hong
authors.initials: YH
authors.lastname: Zhou
authors.affiliation: Han-Xing Tong, Yong Zhang, Jiong-Yuan Wang, Quan Jiang, Jun-Yi He, Ye-Bo Shao, Jun Zhu, Wei-Qi Lu, Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Ying-Yong
authors.initials: YY
authors.lastname: Hou
authors.affiliation: Han-Xing Tong, Yong Zhang, Jiong-Yuan Wang, Quan Jiang, Jun-Yi He, Ye-Bo Shao, Jun Zhu, Wei-Qi Lu, Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Han-Xing Tong, Yong Zhang, Jiong-Yuan Wang, Quan Jiang, Jun-Yi He, Ye-Bo Shao, Jun Zhu, Wei-Qi Lu, Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Han-Xing Tong, Yong Zhang, Jiong-Yuan Wang, Quan Jiang, Jun-Yi He, Ye-Bo Shao, Jun Zhu, Wei-Qi Lu, Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Xie
authors.affiliation: Han-Xing Tong, Yong Zhang, Jiong-Yuan Wang, Quan Jiang, Jun-Yi He, Ye-Bo Shao, Jun Zhu, Wei-Qi Lu, Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Jiong-Yuan
authors.initials: JY
authors.lastname: Wang
authors.affiliation: Han-Xing Tong, Yong Zhang, Jiong-Yuan Wang, Quan Jiang, Jun-Yi He, Ye-Bo Shao, Jun Zhu, Wei-Qi Lu, Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Jiang
authors.affiliation: Han-Xing Tong, Yong Zhang, Jiong-Yuan Wang, Quan Jiang, Jun-Yi He, Ye-Bo Shao, Jun Zhu, Wei-Qi Lu, Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Jun-Yi
authors.initials: JY
authors.lastname: He
authors.affiliation: Han-Xing Tong, Yong Zhang, Jiong-Yuan Wang, Quan Jiang, Jun-Yi He, Ye-Bo Shao, Jun Zhu, Wei-Qi Lu, Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Ye-Bo
authors.initials: YB
authors.lastname: Shao
authors.affiliation: Han-Xing Tong, Yong Zhang, Jiong-Yuan Wang, Quan Jiang, Jun-Yi He, Ye-Bo Shao, Jun Zhu, Wei-Qi Lu, Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Wu-Mei
authors.initials: WM
authors.lastname: Han
authors.affiliation: Han-Xing Tong, Yong Zhang, Jiong-Yuan Wang, Quan Jiang, Jun-Yi He, Ye-Bo Shao, Jun Zhu, Wei-Qi Lu, Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Ruo-Ying
authors.initials: RY
authors.lastname: Tan
authors.affiliation: Han-Xing Tong, Yong Zhang, Jiong-Yuan Wang, Quan Jiang, Jun-Yi He, Ye-Bo Shao, Jun Zhu, Wei-Qi Lu, Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Han-Xing Tong, Yong Zhang, Jiong-Yuan Wang, Quan Jiang, Jun-Yi He, Ye-Bo Shao, Jun Zhu, Wei-Qi Lu, Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Wei-Qi
authors.initials: WQ
authors.lastname: Lu
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics*; D005260:Female; D005770:Gastrointestinal Neoplasms / Q000235:genetics* / Q000473:pathology; D046152:Gastrointestinal Stromal Tumors / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D011237:Predictive Value of Tests; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs
keywords: Diagnosis; Gastrointestinal stromal tumors; MicroRNAs; Microarray analysis; Real-time polymerase chain reaction