title: Plasmodium falciparum genetic crosses in a humanized mouse model.
abstract: Genetic crosses of phenotypically distinct strains of the human malaria parasite Plasmodium falciparum are a powerful tool for identifying genes controlling drug resistance and other key phenotypes. Previous studies relied on the isolation of recombinant parasites from splenectomized chimpanzees, a research avenue that is no longer available. Here we demonstrate that human-liver chimeric mice support recovery of recombinant progeny for the identification of genetic determinants of parasite traits and adaptations.
authors.affiliation: Center for Infectious Disease Research (formerly Seattle Biomedical Research Institute), Seattle, Washington, USA.
authors.forename: Ashley M
authors.initials: AM
authors.lastname: Vaughan
authors.affiliation: Eck Institute for Global Health, Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana, USA.
authors.forename: Richard S
authors.identifier: 0000000177634800
authors.initials: RS
authors.lastname: Pinapati
authors.affiliation: Texas Biomedical Research Institute, San Antonio, Texas, USA.
authors.forename: Ian H
authors.initials: IH
authors.lastname: Cheeseman
authors.affiliation: Center for Infectious Disease Research (formerly Seattle Biomedical Research Institute), Seattle, Washington, USA.
authors.forename: Nelly
authors.initials: N
authors.lastname: Camargo
authors.affiliation: Center for Infectious Disease Research (formerly Seattle Biomedical Research Institute), Seattle, Washington, USA.
authors.forename: Matthew
authors.initials: M
authors.lastname: Fishbaugher
authors.affiliation: Eck Institute for Global Health, Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana, USA.
authors.forename: Lisa A
authors.initials: LA
authors.lastname: Checkley
authors.affiliation: Texas Biomedical Research Institute, San Antonio, Texas, USA.
authors.forename: Shalini
authors.initials: S
authors.lastname: Nair
authors.affiliation: Eck Institute for Global Health, Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana, USA.
authors.forename: Carolyn A
authors.initials: CA
authors.lastname: Hutyra
authors.affiliation: Shoklo Malaria Research Unit, Mahidol-Oxford Tropical Medicine Research Unit, Mahidol University, Mae Sot, Thailand.
authors.forename: François H
authors.initials: FH
authors.lastname: Nosten
authors.affiliation: Texas Biomedical Research Institute, San Antonio, Texas, USA.
authors.forename: Timothy J C
authors.initials: TJ
authors.lastname: Anderson
authors.affiliation: Eck Institute for Global Health, Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana, USA.
authors.forename: Michael T
authors.initials: MT
authors.lastname: Ferdig
authors.affiliation: 1] Center for Infectious Disease Research (formerly Seattle Biomedical Research Institute), Seattle, Washington, USA. [2] Department of Global Health, University of Washington, Seattle, Washington, USA.
authors.forename: Stefan H I
authors.initials: SH
authors.lastname: Kappe
mesh_terms: D000818:Animals; D037621:Artemisinins / Q000494:pharmacology; D003433:Crosses, Genetic; D004351:Drug Resistance; D006801:Humans; D051379:Mice; D010963:Plasmodium falciparum / Q000187:drug effects / Q000235:genetics*
chemical_list: D037621:Artemisinins; C031327:artemisinin