title: Automated cell viability assessment using a microfluidics based portable imaging flow analyzer.
abstract: In this work, we report a system-level integration of portable microscopy and microfluidics for the realization of optofluidic imaging flow analyzer with a throughput of 450 cells/s. With the use of a cellphone augmented with off-the-shelf optical components and custom designed microfluidics, we demonstrate a portable optofluidic imaging flow analyzer. A multiple microfluidic channel geometry was employed to demonstrate the enhancement of throughput in the context of low frame-rate imaging systems. Using the cell-phone based digital imaging flow analyzer, we have imaged yeast cells present in a suspension. By digitally processing the recorded videos of the flow stream on the cellphone, we demonstrated an automated cell viability assessment of the yeast cell population. In addition, we also demonstrate the suitability of the system for blood cell counting.
authors.affiliation: Optics and Microfluidics Instrumentation Lab, Department of Instrumentation and Applied Physics, Indian Institute of Science , Bangalore 560012, India.
authors.forename: Veerendra Kalyan
authors.initials: VK
authors.lastname: Jagannadh
authors.affiliation: Optics and Microfluidics Instrumentation Lab, Department of Instrumentation and Applied Physics, Indian Institute of Science , Bangalore 560012, India.
authors.forename: Jayesh Vasudeva
authors.initials: JV
authors.lastname: Adhikari
authors.affiliation: Optics and Microfluidics Instrumentation Lab, Department of Instrumentation and Applied Physics, Indian Institute of Science , Bangalore 560012, India.
authors.forename: Sai Siva
authors.initials: SS
authors.lastname: Gorthi