title: Platelet-Rich Plasma Increases Growth and Motility of Adipose Tissue-Derived Mesenchymal Stem Cells and Controls Adipocyte Secretory Function.
abstract: Adipose tissue-derived mesenchymal stem cells (Ad-MSC) and platelet derivatives have been used alone or in combination to achieve regeneration of injured tissues. We have tested the effect of platelet-rich plasma (PRP) on Ad-MSC and adipocyte function. PRP increased Ad-MSC viability, proliferation rate and G1-S cell cycle progression, by at least 7-, 2-, and 2.2-fold, respectively, and reduced caspase 3 cleavage. Higher PRP concentrations or PRPs derived from individuals with higher platelet counts were more effective in increasing Ad-MSC growth. PRP also accelerated cell migration by at least 1.5-fold. However, PRP did not significantly affect mature adipocyte viability, differentiation and expression levels of PPAR-γ and AP-2 mRNAs, while it increased leptin production by 3.5-fold. Interestingly, PRP treatment of mature adipocytes also enhanced the release of Interleukin (IL)-6, IL-8, IL-10, Interferon-γ, and Vascular Endothelial Growth Factor. Thus, data are consistent with a stimulatory effect of platelet derivatives on Ad-MSC growth and motility. Moreover, PRP did not reduce mature adipocyte survival and increased the release of pro-angiogenic factors, which may facilitate tissue regeneration processes.
authors.affiliation: Department of Translational Medicine, Federico II, University of Naples, Naples, Italy.
authors.forename: Vittoria
authors.initials: V
authors.lastname: D'Esposito
authors.affiliation: Department of Translational Medicine, Federico II, University of Naples, Naples, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Passaretti
authors.affiliation: Institute of Experimental Endocrinology and Oncology, National Council of Research (CNR), Naples, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Perruolo
authors.affiliation: Department of Translational Medicine, Federico II, University of Naples, Naples, Italy.
authors.forename: Maria Rosaria
authors.initials: MR
authors.lastname: Ambrosio
authors.affiliation: Institute of Experimental Endocrinology and Oncology, National Council of Research (CNR), Naples, Italy.
authors.forename: Rossella
authors.initials: R
authors.lastname: Valentino
authors.affiliation: Department of Translational Medicine, Federico II, University of Naples, Naples, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Oriente
authors.affiliation: Institute of Experimental Endocrinology and Oncology, National Council of Research (CNR), Naples, Italy.
authors.forename: Gregory A
authors.initials: GA
authors.lastname: Raciti
authors.affiliation: Institute of Experimental Endocrinology and Oncology, National Council of Research (CNR), Naples, Italy.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Nigro
authors.affiliation: Institute of Experimental Endocrinology and Oncology, National Council of Research (CNR), Naples, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Miele
authors.affiliation: Department of Neurosciences, Reproductive and Odonto-stomatological Sciences, Federico II University of Naples, Naples, Italy.
authors.forename: Gilberto
authors.initials: G
authors.lastname: Sammartino
authors.affiliation: Department of Translational Medicine, Federico II, University of Naples, Naples, Italy.|Institute of Experimental Endocrinology and Oncology, National Council of Research (CNR), Naples, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Beguinot
authors.affiliation: Department of Translational Medicine, Federico II, University of Naples, Naples, Italy.|Institute of Experimental Endocrinology and Oncology, National Council of Research (CNR), Naples, Italy.
authors.forename: Pietro
authors.initials: P
authors.lastname: Formisano
mesh_terms: D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D006801:Humans; D007378:Interleukins / Q000096:biosynthesis; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D047495:PPAR gamma / Q000096:biosynthesis; D053657:Platelet-Rich Plasma; D012038:Regeneration
chemical_list: D007378:Interleukins; D047495:PPAR gamma
keywords: ADIPOSE TISSUE; CYTOKINES; GROWTH FACTORS; TISSUE REGENERATION