title: [Detection of pathogenic bacteria Rhizobium vitis in vineyards of the south of Ukraine].
abstract: The total number of microbiota from grape crown gall tissues ranged from (2,3 ± 0.8) x 10(3) to (7.3 ± 0.4) x 10(5) CFU/g. The amount of bacteria from Rhizobium genus reached from (1.2 ±0.2) x 10(2) to (2.1 ± 0.2) x 10(4) CFU/g depending on a tested plant. It was found out that only a small percentage of the strains (3.1 - 4.9%) were oncogenic. New pathogenic strains (R. vitis ONU388, R. vitis ONU389 and R. vitis ONU390) possessing plasmid genes of pathogenicity virC, ipt and virD2 were isolated.
authors.lastname: Korotaieva N V
authors.lastname: Limanska N V
mesh_terms: D017931:DNA Primers / Q000138:chemical synthesis; D004269:DNA, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D018517:Plant Roots / Q000382:microbiology*; D010941:Plant Tumors / Q000382:microbiology*; D010957:Plasmids / Q000737:chemistry / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012231:Rhizobium / Q000235:genetics / Q000472:pathogenicity*; D014455:Ukraine; D027843:Vitis / Q000382:microbiology*
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial