title: Fungal invasion of the rhizosphere microbiome.
abstract: The rhizosphere is the infection court where soil-borne pathogens establish a parasitic relationship with the plant. To infect root tissue, pathogens have to compete with members of the rhizosphere microbiome for available nutrients and microsites. In disease-suppressive soils, pathogens are strongly restricted in growth by the activities of specific rhizosphere microorganisms. Here, we sequenced metagenomic DNA and RNA of the rhizosphere microbiome of sugar beet seedlings grown in a soil suppressive to the fungal pathogen Rhizoctonia solani. rRNA-based analyses showed that Oxalobacteraceae, Burkholderiaceae, Sphingobacteriaceae and Sphingomonadaceae were significantly more abundant in the rhizosphere upon fungal invasion. Metatranscriptomics revealed that stress-related genes (ppGpp metabolism and oxidative stress) were upregulated in these bacterial families. We postulate that the invading pathogenic fungus induces, directly or via the plant, stress responses in the rhizobacterial community that lead to shifts in microbiome composition and to activation of antagonistic traits that restrict pathogen infection.
authors.affiliation: Laboratory of Phytopathology, Wageningen University, Wageningen, The Netherlands.
authors.forename: Emilie
authors.initials: E
authors.lastname: Chapelle
authors.affiliation: Laboratory of Phytopathology, Wageningen University, Wageningen, The Netherlands.|Laboratory of Environmental Microbiology, Brazilian Agricultural Research Corporation, Embrapa Environment, Jaguariuna, Brazil.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Mendes
authors.affiliation: Plant-Microbe Interactions, Department of Biology, Utrecht University, Utrecht, The Netherlands.
authors.forename: Peter A H M
authors.initials: PA
authors.lastname: Bakker
authors.affiliation: Laboratory of Phytopathology, Wageningen University, Wageningen, The Netherlands.|Department of Microbial Ecology, Netherlands Institute of Ecology (NIOO-KNAW), Wageningen, The Netherlands.
authors.forename: Jos M
authors.initials: JM
authors.lastname: Raaijmakers
mesh_terms: D000898:Antibiosis; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D018407:Bacterial Physiological Phenomena; D056186:Metagenomics; D064307:Microbiota; D018517:Plant Roots / Q000382:microbiology; D010944:Plants / Q000382:microbiology; D012232:Rhizoctonia / Q000502:physiology*; D058441:Rhizosphere; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology
chemical_list: D012987:Soil