title: Microarray Gene-expression Profiling Analysis Comparing PCNSL and Non-CNS Diffuse Large B-Cell Lymphoma.
abstract: OBJECTIVE
  The purpose of the present study was to compare microarray gene-expression profiling data between primary central nervous system (CNS) lymphoma and non-CNS lymphomas.
  
  
  METHODS
  We performed whole-genomic cDNA-mediated annealing, selection and ligation assay with 177 formalin-fixed paraffin-embedded tumor samples.
  
  
  RESULTS
  We identified 20 differentially expressed genes out of which 5 were predominantly expressed in CNS DLBCL compared to non-CNS DLBCL (C16orf59, SLC16A9, HPDL, SPP1, and MAG). SLC16A9 may be involved in aerobic glycolysis of malignant tumors. The alteration in gene expression of SPP1 in primary CNS lymphoma is involved in biological activity, such as CNS tropism, B-cell migration, proliferation, and aggressive clinical behavior. MAG may be an important adhesion molecule that contributes to perineural cancer invasion.
  
  
  CONCLUSIONS
  Genomic differences between CNS and non-CNS DLBCL exist and the most prominent genes are SPP1 and MAG. SPP1 may play a key role in CNS tropism of primary CNS lymphoma.
authors.affiliation: Division of Hematology-Oncology, Department of Medicine, Dankook University College of Medicine, Cheonan, Republic of Korea.
authors.forename: Do Hyoung
authors.initials: DH
authors.lastname: Lim
authors.affiliation: Division of Hematology-Oncology, Department of Medicine, Samsung Medical Center Sungkyunkwan University School of Medicine, Seoul, Republic of Korea wskimsmc@skku.edu.
authors.forename: Won Seog
authors.initials: WS
authors.lastname: Kim
authors.affiliation: Division of Hematology-Oncology, Department of Medicine, Samsung Medical Center Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.
authors.forename: Seok Jin
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: Department of Biomedical Sciences, Samsung Research Institute for Future Medicine, Samsung Medical Center, Seoul, Republic of Korea.
authors.forename: Hae Yong
authors.initials: HY
authors.lastname: Yoo
authors.affiliation: Department of Pathology, Samsung Medical Center Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.
authors.forename: Young Hyeh
authors.initials: YH
authors.lastname: Ko
mesh_terms: D000368:Aged; D001402:B-Lymphocytes / Q000378:metabolism / Q000473:pathology; D014408:Biomarkers, Tumor / Q000096:biosynthesis*; D016543:Central Nervous System Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics* / Q000473:pathology; D008297:Male; D046228:Microarray Analysis; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis*
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins
keywords: Diffuse large B-cell lymphoma; gene expression; microarray; primary CNS lymphoma