title: [The development of biochip to detect anti-cholera antibodies in human blood serum].
abstract: The full-scaled agglutinating immunoassay is commonly applied to detect content of antibodies to cholera agent Vibrio cholerae human in blood serum under application of serological diagnostic. The time of analysis implementation amounts to 18 hours. To shorten time of detection of antibodies a biological microchip (biochip) was developed. The biochip represents an activated slide with immobilized corpuscle and soluble antigen cholera agent (O-antigens, cholera toxin). The experimental work resulted in development of scheme of biochip and selection of optimal conditions of sorption and implementation of immunologic analysis using biochip. The application of biochip facilitated to detect specific antibodies to antigens of cholera agent in commercial experimental animal serums and blood serums of ill patients. The time of analysis implementation amounted to 2-3 hours. The results are substantiated by bacteriological and serological methods.
authors.forename: D V
authors.initials: DV
authors.lastname: Utkin
authors.forename: N A
authors.initials: NA
authors.lastname: Osina
authors.forename: A N
authors.initials: AN
authors.lastname: Spitsin
authors.forename: M N
authors.initials: MN
authors.lastname: Kireev
authors.forename: O V
authors.initials: OV
authors.lastname: Gromova
authors.forename: T L
authors.initials: TL
authors.lastname: Zakharova
authors.forename: E V
authors.initials: EV
authors.lastname: Naidenova
authors.forename: V E
authors.initials: VE
authors.lastname: Kuklev
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood* / Q000276:immunology; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology; D002771:Cholera / Q000097:blood* / Q000276:immunology / Q000382:microbiology; D002772:Cholera Toxin / Q000737:chemistry / Q000276:immunology; D006801:Humans; D040081:Protein Array Analysis / Q000295:instrumentation*; D014734:Vibrio cholerae / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D002772:Cholera Toxin