title: Determination of Triphenylmethane Dyes and Their Metabolites in Salmon, Catfish, and Shrimp by LC-MS/MS Using AOAC First Action Method 2012.25: Collaborative Study.
abstract: A collaborative study was conducted to evaluate the AOAC First Action 2012.25 LC-MS/MS analytical method for the determination of residues of three triphenylmethane dyes (malachite green, crystal violet, and brilliant green) and their metabolites (leucomalachite green and leucocrystal violet) in seafood. Fourteen laboratories from the United States, Canada, and the European Union member states participated in the study including national and state regulatory laboratories, university and national research laboratories, and private analytical testing laboratories. A variety of LC-MS/MS instruments were used for the analysis. Each participating laboratory received blinded test samples in duplicate of salmon, catfish, and shrimp consisting of negative control matrix; matrix fortified with residues at 0.42, 0.90, and 1.75 μg/kg; and samples of incurred matrix. The analytical results from each participating laboratory were evaluated for both quantitative residue determination and qualitative identification of targeted analytes. Results from statistical analysis showed that this method provided excellent trueness (generally ≥90% recovery) and precision (RSDr generally ≤10%, HorRat<1). The Study Directors recommend Method 2012.25 for Final Action status.
authors.affiliation: U.S. Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 East Mermaid Ln, Wyndmoor, PA 19038, USA.
authors.forename: Marilyn J
authors.initials: MJ
authors.lastname: Schneider
authors.forename: Wendy C
authors.initials: WC
authors.lastname: Andersen
mesh_terms: D000818:Animals; D002138:Calibration; D002397:Catfishes / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004396:Coloring Agents / Q000032:analysis*; D033561:Penaeidae / Q000737:chemistry / Q000378:metabolism*; D012015:Reference Standards; D012474:Salmon / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D014320:Triphenylmethyl Compounds / Q000032:analysis*
chemical_list: D004396:Coloring Agents; D014320:Triphenylmethyl Compounds; C046945:triphenylmethane