title: The pharmacokinetic study on the mechanism of toxicity attenuation of rhubarb total free anthraquinone oral colon-specific drug delivery system.
abstract: Rhubarb is commonly used as laxatives in Asian countries, of which anthraquinones are the major active ingredients, but there are an increased number of concerns regarding the nephrotoxicity of anthraquinones. In this study, we compared the pharmacokinetic characteristics of rhubarb anthraquinones in rats after orally administered with rhubarb and rhubarb total free anthraquinone oral colon-specific drug delivery granules (RTFA-OCDD-GN), and then explained why these granules could reduce the nephrotoxicity of anthraquinones when they produced purgative efficacy. A sensitive and reliable high performance liquid chromatography (HPLC) method has been fully validated for simultaneous determination of the five active components of rhubarb, and successfully applied to investigate and compare the remarkable differences in pharmacokinetic study of rhubarb anthraquinones after orally administered with rhubarb and RTFA-OCDD-GN. The results showed that, compared with rhubarb group, the AUC, Cmax, t1/2z and Vz/F of aloe-emodin, rhein, emodin and chrysophanol in rats receiving the RTFA-OCDD-GN were significantly decreased, and the Tmax of the four analytes was prolonged. Moreover, the Tmax of rhein, the Cmax of chrysophanol and emodin all have significant differences (P<0.05). Simultaneously, anthraquinone prototype excretion rates in urine and feces of aloe-emodin, rhein, emodin, chrysophanol and physcion were all increased. These findings suggested that oral colon-specific drug delivery technology made anthraquinone aglycone to colon-specific release after oral administration. This allowed anthraquinones to not only play the corresponding purgative effect but also avoid intestinal absorption and promote excretion. And thereby greatly reduced the nephrotoxicity of rhubarb. The result is a new breakthrough in rhubarb toxicity attenuated research.
authors.affiliation: Hebei Province key Laboratory of Research and Development for Chinese Medicine, Chengde Medical College, Chengde, Hebei 067000, P. R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Hebei Province key Laboratory of Research and Development for Chinese Medicine, Chengde Medical College, Chengde, Hebei 067000, P. R. China.
authors.forename: Jin-hua
authors.initials: JH
authors.lastname: Chang
authors.affiliation: Affiliated Hospital of Chengde Medical College, Chengde, Hebei 067000, P. R. China.
authors.forename: Bao-qi
authors.initials: BQ
authors.lastname: Zhang
authors.affiliation: Hebei Province key Laboratory of Research and Development for Chinese Medicine, Chengde Medical College, Chengde, Hebei 067000, P. R. China.
authors.forename: Xi-gang
authors.initials: XG
authors.lastname: Liu
authors.affiliation: Hebei Province key Laboratory of Research and Development for Chinese Medicine, Chengde Medical College, Chengde, Hebei 067000, P. R. China.
authors.forename: Pei
authors.initials: P
authors.lastname: Liu
authors.affiliation: Hebei Province key Laboratory of Research and Development for Chinese Medicine, Chengde Medical College, Chengde, Hebei 067000, P. R. China.
authors.forename: He-fei
authors.initials: HF
authors.lastname: Xue
authors.affiliation: Hebei Province key Laboratory of Research and Development for Chinese Medicine, Chengde Medical College, Chengde, Hebei 067000, P. R. China.
authors.forename: Li-yan
authors.initials: LY
authors.lastname: Liu
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, P. R. China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, P. R. China.
authors.forename: Meng
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Hebei Province key Laboratory of Research and Development for Chinese Medicine, Chengde Medical College, Chengde, Hebei 067000, P. R. China. Electronic address: liucuizhexy@163.com.
authors.forename: Cui-zhe
authors.initials: CZ
authors.lastname: Liu
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000880:Anthraquinones / Q000493:pharmacokinetics*; D002400:Cathartics / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid; D003106:Colon; D016503:Drug Delivery Systems; D007408:Intestinal Absorption; D007668:Kidney / Q000187:drug effects; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012250:Rheum / Q000737:chemistry*; D018675:Toxicity Tests
chemical_list: D000880:Anthraquinones; D002400:Cathartics
keywords: Oral colon-specific drug delivery system; Pharmacokinetics; Rhubarb anthraquinones; Toxicity attenuation