title: Isolation and cDNA characteristics ofMHC-DRAgenes from gayal (Bos frontalis) and gaytle (Bos frontalis×Bos taurus).
abstract: The mammalian major histocompatibility complex (MHC) plays important roles in pathogen recognition and disease resistance. In the present study, the coding sequence and the 5'- and 3'-untranslated regions of MHC class II DR alpha chain (the DRA gene) from rare gayal and gaytle were cloned and analyzed to dissect structural and functional variations. The nucleotide and amino acid sequences for theDRAgenes in gayal (Bofr-DRA) and gaytle (Bofr×BoLA-DRA) were almost identical to those for cattle and yak (99%). Compared to yak, two amino acids substitutions in the signal peptide (SP) domain for gayal were found within allBosanimals. Except for only one replacement in the amino acid within the α2 domain of the DRA protein in gayal, the additional residues were highly conserved across the species investigated. The 20 peptide-binding sites (PBS) ofBofr-DRAandBofr×BoLA-DRAwere essentially reserved in the α1 domain among all species investigated. The lesser degree of substitution inBofr-DRAis concordant with the concept that theDRAgene is highly conserved among all mammals. The very high degree of conservativity of theDRAgene among ruminants, including gayal, suggests its recent evolutionary separation.
authors.affiliation: Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming , Yunnan , P.R. China.
authors.forename: Yongke
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming , Yunnan , P.R. China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming , Yunnan , P.R. China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Xi
authors.affiliation: Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming , Yunnan , P.R. China.
authors.forename: Guozhi
authors.initials: G
authors.lastname: Li
authors.affiliation: Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming , Yunnan , P.R. China.
authors.forename: Liping
authors.initials: L
authors.lastname: Wang
authors.affiliation: Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming , Yunnan , P.R. China.
authors.forename: Huanli
authors.initials: H
authors.lastname: Zheng
authors.affiliation: Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming , Yunnan , P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Du
authors.affiliation: Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming , Yunnan , P.R. China.
authors.forename: Zhaobing
authors.initials: Z
authors.lastname: Gu
authors.affiliation: Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming , Yunnan , P.R. China.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming , Yunnan , P.R. China.
authors.forename: Yuai
authors.initials: Y
authors.lastname: Yang
keywords: DRA gene; functional domains; gayal (Bos frontalis); gaytle (Bos frontalis × Bos taurus)