title: Interaction of sulforaphane with DNA and RNA.
abstract: Sulforaphane (SFN) is an isothiocyanate found in cruciferous vegetables with anti-inflammatory, anti-oxidant and anti-cancer activities. However, the antioxidant and anticancer mechanism of sulforaphane is not well understood. In the present research, we reported binding modes, binding constants and stability of SFN-DNA and -RNA complexes by Fourier transform infrared (FTIR) and UV-Visible spectroscopic methods. Spectroscopic evidence showed DNA intercalation with some degree of groove binding. SFN binds minor and major grooves of DNA and backbone phosphate (PO2), while RNA binding is through G, U, A bases with some degree of SFN-phosphate (PO2) interaction. Overall binding constants were estimated to be K(SFN-DNA)=3.01 (± 0.035)×10(4) M(-1) and K(SFN-RNA)= 6.63 (±0.042)×10(3) M(-1). At high SFN concentration (SFN/RNA = 1/1), DNA conformation changed from B to A occurred, while RNA remained in A-family structure.
authors.affiliation: Department of Biology, IAU, Science and Research Branch, Tehran, Iran.
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Abassi Joozdani
authors.affiliation: Department of Biology, IAU, Science and Research Branch, Tehran, Iran.
authors.forename: Faramarz
authors.initials: F
authors.lastname: Yari
authors.affiliation: Department of Biology, IAU, Science and Research Branch, Tehran, Iran.
authors.forename: Parvaneh
authors.initials: P
authors.lastname: Abassi Joozdani
authors.affiliation: Department of Chemistry, IAU, Central Tehran Branch, Tehran, Iran; Department of Dermatology, University of California, San Francisco, California, United States of America.
authors.forename: Shohreh
authors.initials: S
authors.lastname: Nafisi
mesh_terms: D004247:DNA / Q000737:chemistry*; D017879:Isothiocyanates / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013454:Sulfoxides
chemical_list: D017879:Isothiocyanates; D013454:Sulfoxides; D012313:RNA; D004247:DNA; C016766:sulforaphane