title: Combination Patterns of Major R Genes Determine the Level of Resistance to the M. oryzae in Rice (Oryza sativa L.).
abstract: Rice blast caused by Magnaporthe oryzae is the most devastating disease of rice and poses a serious threat to world food security. In this study, the distribution and effectiveness of 18 R genes in 277 accessions were investigated based on pathogenicity assays and molecular markers. The results showed that most of the accessions exhibited some degree of resistance (resistance frequency, RF >50%). Accordingly, most of the accessions were observed to harbor two or more R genes, and the number of R genes harbored in accessions was significantly positively correlated with RF. Some R genes were demonstrated to be specifically distributed in the genomes of rice sub-species, such as Pigm, Pi9, Pi5 and Pi1, which were only detected in indica-type accessions, and Pik and Piz, which were just harbored in japonica-type accessions. By analyzing the relationship between R genes and RF using a multiple stepwise regression model, the R genes Pid3, Pi5, Pi9, Pi54, Pigm and Pit were found to show the main effects against M. oryzae in indica-type accessions, while Pita, Pb1, Pik, Pizt and Pia were indicated to exhibit the main effects against M. oryzae in japonica-type accessions. Principal component analysis (PCA) and cluster analysis revealed that combination patterns of major R genes were the main factors determining the resistance of rice varieties to M. oryzae, such as 'Pi9+Pi54', 'Pid3+Pigm', 'Pi5+Pid3+Pigm', 'Pi5+Pi54+Pid3+Pigm', 'Pi5+Pid3' and 'Pi5+Pit+Pid3' in indica-type accessions and 'Pik+Pib', 'Pik+Pita', 'Pik+Pb1', 'Pizt+Pia' and 'Pizt+Pita' in japonica-type accessions, which were able to confer effective resistance against M. oryzae. The above results provide good theoretical support for the rational utilization of combinations of major R genes in developing rice cultivars with broad-spectrum resistance.
authors.affiliation: Lixiahe Agricultural Research Institute of Jiangsu Province, Yangzhou, 225007, P.R. China; Key Laboratory of Plant Functional Genomics, Ministry of Education, Yangzhou University, Yangzhou, 225009, P.R. China.
authors.forename: Yunyu
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Lixiahe Agricultural Research Institute of Jiangsu Province, Yangzhou, 225007, P.R. China.
authors.forename: Ning
authors.initials: N
authors.lastname: Xiao
authors.affiliation: Lixiahe Agricultural Research Institute of Jiangsu Province, Yangzhou, 225007, P.R. China.
authors.forename: Ling
authors.initials: L
authors.lastname: Yu
authors.affiliation: Lixiahe Agricultural Research Institute of Jiangsu Province, Yangzhou, 225007, P.R. China.
authors.forename: Cunhong
authors.initials: C
authors.lastname: Pan
authors.affiliation: Lixiahe Agricultural Research Institute of Jiangsu Province, Yangzhou, 225007, P.R. China.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Lixiahe Agricultural Research Institute of Jiangsu Province, Yangzhou, 225007, P.R. China.
authors.forename: Xiaoxiang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Lixiahe Agricultural Research Institute of Jiangsu Province, Yangzhou, 225007, P.R. China.
authors.forename: Guangqing
authors.initials: G
authors.lastname: Liu
authors.affiliation: Lixiahe Agricultural Research Institute of Jiangsu Province, Yangzhou, 225007, P.R. China.
authors.forename: Zhengyuan
authors.initials: Z
authors.lastname: Dai
authors.affiliation: Key Laboratory of Plant Functional Genomics, Ministry of Education, Yangzhou University, Yangzhou, 225009, P.R. China.
authors.forename: Xuebiao
authors.initials: X
authors.lastname: Pan
authors.affiliation: Lixiahe Agricultural Research Institute of Jiangsu Province, Yangzhou, 225007, P.R. China.
authors.forename: Aihong
authors.initials: A
authors.lastname: Li
mesh_terms: D060467:Disease Resistance / Q000235:genetics / Q000502:physiology; D020082:Magnaporthe / Q000472:pathogenicity*; D012275:Oryza / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D010940:Plant Proteins