title: [Expressions of CDX2 and β-catenin and their correlation in acute myeloid leukemia].
abstract: OBJECTIVE
  To investigate the expressions of CDX2 and β-catenin and their correlation in acute myeloid leukemia (AML).
  
  
  METHODS
  Real-time PCR was used to examine the expressions of CDX2 and β-catenin mRNA in 92 de novo AML and 30 non-malignant patients (control), and Western blot was employed to detect CDX2 and β-catenin protein expressions in 26 de novo AML and 8 non-malignant patients.
  
  
  RESULTS
  The positivity rates of CDX2 mRNA and protein expressions in AML group were 84.78% and 76.92%, respectively, but no CDX2 mRNA or protein expression were detected in control group (P<0.01). The expressions of β-catenin mRNA and protein were detected in all the patients in both groups but its expressions in AML group were significantly higher (P<0.01). The expressions of CDX2 and β-catenin mRNA were significantly correlated with WBC counts and LDH levels (P<0.01). The expression of CDX2 mRNA was not detected and the expression of β-catenin mRNA was decreased to normal level in AML patients with complete remission, while the expressions of CDX2 and β-catenin mRNA were increased again in those with disease relapse. There were significantly positive correlations between CDX2 and β-catenin expressions at both mRNA and protein levels (P<0.01).
  
  
  CONCLUSIONS
  Up-regulation of CDX2 gene and activation of Wnt/β-catenin pathway co-exist in AML patients and the expressions of CDX2 and β-catenin are positively correlated.
authors.affiliation: Laboratory of Hematology, Guangdong Medical University, Zhanjiang 524000, China.E-mail: 532723336@qq.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Can
authors.initials: C
authors.lastname: Meng
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Yang
mesh_terms: D015153:Blotting, Western; D000071616:CDX2 Transcription Factor; D018398:Homeodomain Proteins / Q000378:metabolism*; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism*; D012333:RNA, Messenger; D015854:Up-Regulation; D060449:Wnt Signaling Pathway; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D000071616:CDX2 Transcription Factor; C491270:CDX2 protein, human; C495270:CTNNB1 protein, human; D018398:Homeodomain Proteins; D012333:RNA, Messenger; D051176:beta Catenin