title: Contact toxicity and repellency of the essential oil from Mentha haplocalyx Briq. against Lasioderma serricorne.
abstract: The chemical composition of the essential oil obtained by hydrodistillation from the aerial parts of Mentha haplocalyx was investigated by GC-FID and GC/MS analyses. In sum, 23 components, representing 92.88% of the total oil composition, were identified, and the main compounds were found to be menthol (59.71%), menthyl acetate (7.83%), limonene (6.98%), and menthone (4.44%). By bioassay-guided fractionation (contact toxicity), three compounds were obtained from the essential oil and identified as menthol, menthyl acetate, and limonene. The essential oil and the three isolated compounds exhibited potent contact toxicity against Lasioderma serricorne adults, with LD50 values of 16.5, 7.91, 5.96, and 13.7 μg/adult, respectively. Moreover, the oil and its isolated compounds also exhibited strong repellency against L. serricorne adults. At the lower concentrations tested and at 2 h after exposure, menthol showed even significantly stronger repellency than the positive control DEET. The study revealed that the bioactivity properties of the essential oil can be attributed to the synergistic effects of its diverse major and minor components, which indicates that the M. haplocalyx oil and its isolated compounds have potential for the development as natural insecticides and/or repellents to control insects in stored grains and traditional Chinese medicinal materials.
authors.affiliation: Beijing Key Laboratory of Traditional Chinese Medicine Protection and Utilization, Beijing Normal University, No. 19 Xinjiekouwai Street, Beijing 100875, P. R. China, (phone/fax: +86-10-62208022).
authors.forename: Wen-Juan
authors.initials: WJ
authors.lastname: Zhang
authors.affiliation: Beijing Key Laboratory of Traditional Chinese Medicine Protection and Utilization, Beijing Normal University, No. 19 Xinjiekouwai Street, Beijing 100875, P. R. China, (phone/fax: +86-10-62208022).
authors.forename: Kai
authors.initials: K
authors.lastname: Yang
authors.affiliation: Beijing Key Laboratory of Traditional Chinese Medicine Protection and Utilization, Beijing Normal University, No. 19 Xinjiekouwai Street, Beijing 100875, P. R. China, (phone/fax: +86-10-62208022).
authors.forename: Chun-Xue
authors.initials: CX
authors.lastname: You
authors.affiliation: Beijing Key Laboratory of Traditional Chinese Medicine Protection and Utilization, Beijing Normal University, No. 19 Xinjiekouwai Street, Beijing 100875, P. R. China, (phone/fax: +86-10-62208022). wangchengfang@mail.bnu.edu.cn.|China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Xicheng District, Beijing 100088, P. R. China (phone/fax: +86-10-62208022). wangchengfang@mail.bnu.edu.cn.
authors.forename: Cheng-Fang
authors.initials: CF
authors.lastname: Wang
authors.affiliation: Analytical and Testing Center, Beijing Normal University, No. 19 Xinjiekouwai Street, Beijing 100875, P. R. China.
authors.forename: Zhu-Feng
authors.initials: ZF
authors.lastname: Geng
authors.affiliation: Tibet Judicial Police Hospital, No. 10 Zhaji Road, Chengguan District, Lhasa 850000, Tibet Autonomous Region, P. R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Su
authors.affiliation: Beijing Key Laboratory of Traditional Chinese Medicine Protection and Utilization, Beijing Normal University, No. 19 Xinjiekouwai Street, Beijing 100875, P. R. China, (phone/fax: +86-10-62208022).
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Beijing Key Laboratory of Traditional Chinese Medicine Protection and Utilization, Beijing Normal University, No. 19 Xinjiekouwai Street, Beijing 100875, P. R. China, (phone/fax: +86-10-62208022). dushushan@bnu.edu.cn.
authors.forename: Shu-Shan
authors.initials: SS
authors.lastname: Du
authors.affiliation: Analytical and Testing Center, Beijing Normal University, No. 19 Xinjiekouwai Street, Beijing 100875, P. R. China.
authors.forename: Zhi-Wei
authors.initials: ZW
authors.lastname: Deng
mesh_terms: D000818:Animals; D001517:Coleoptera / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D008401:Gas Chromatography-Mass Spectrometry; D007302:Insect Repellents / Q000737:chemistry / Q000633:toxicity*; D007306:Insecticides / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D027541:Mentha / Q000737:chemistry*; D009822:Oils, Volatile / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D010938:Plant Oils / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*
chemical_list: D007302:Insect Repellents; D007306:Insecticides; D009822:Oils, Volatile; D010938:Plant Oils
keywords: Contact toxicity; Essential oils; Lasioderma serricorne; Mentha haplocalyx; Menthol; Repellency