title: Rapid quick, easy, cheap, effective, rugged, and safe extraction with novel phospholipid cleanup: A streamlined ultra high performance liquid chromatography with ultraviolet detection approach for screening polycyclic aromatic hydrocarbons in avian blood cells and plasma.
abstract: A streamlined method has been developed for the isolation and analysis of polycyclic aromatic hydrocarbons in avian blood cells and plasma utilizing quick, easy, cheap, effective, rugged, and safe extraction in combination with novel phospholipid cleanup technology. A variety of traditional extraction and cleanup techniques have been employed in the preparation and analysis of polycyclic aromatic hydrocarbonsin a variety of matrices; liquid-liquid partitioning, solid-phase extractions, gel permeation chromatography, and column chromatography are all effective techniques, however they are laborious and time consuming processes that require large amounts of solvent. Using quick, easy, cheap, effective, rugged, and safe extraction coupled with phospholipid cleanup, samples can be quickly screened while maintaining high throughput and sensitivity. With a liquid chromatography approach, analysis times may be kept short at 16 min while maintaining high analyte recovery. Recoveries in quality control samples ranged from 70 to 109%, with average surrogate recoveries of 80.6 ± 1.10%. The result of using a quick, easy, cheap, effective, rugged, and safe extraction approach in conjunction with phospholipid cleanup is a methodology that significantly reduces sample preparation time and solvent use while maintaining high sensitivity and reproducibility.
authors.affiliation: Center for Environmental Sciences and Engineering, University of Connecticut, Storrs, CT, USA.
authors.forename: Anthony A
authors.initials: AA
authors.lastname: Provatas
authors.affiliation: Department of Chemistry, University of Rhode Island, Kingston, RI, USA.
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Yevdokimov
authors.affiliation: Center for Environmental Sciences and Engineering, University of Connecticut, Storrs, CT, USA.|Department of Chemistry, University of Connecticut, Storrs, CT, USA.
authors.forename: Cory A
authors.initials: CA
authors.lastname: King
authors.affiliation: Center for Environmental Sciences and Engineering, University of Connecticut, Storrs, CT, USA.|Department of Chemistry, University of Connecticut, Storrs, CT, USA.
authors.forename: Emma L
authors.initials: EL
authors.lastname: Gatley
authors.affiliation: Center for Environmental Sciences and Engineering, University of Connecticut, Storrs, CT, USA.|Department of Chemistry, University of Connecticut, Storrs, CT, USA.
authors.forename: James D
authors.initials: JD
authors.lastname: Stuart
authors.affiliation: Biodiversity Research Institute, Portland, ME, USA.
authors.forename: David C
authors.initials: DC
authors.lastname: Evers
authors.affiliation: Center for Environmental Sciences and Engineering, University of Connecticut, Storrs, CT, USA.
authors.forename: Christopher R
authors.initials: CR
authors.lastname: Perkins
mesh_terms: D000818:Animals; D001717:Birds / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057230:Limit of Detection; D010743:Phospholipids / Q000302:isolation & purification*; D011084:Polycyclic Aromatic Hydrocarbons / Q000097:blood*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D010743:Phospholipids; D011084:Polycyclic Aromatic Hydrocarbons
keywords: Blood cells; Phospholipid cleanup / Quick easy cheap effective rugged safe; Polycyclic aromatic hydrocarbons; Ultra high performance liquid chromatography