title: The significance of evalution of haematocrit in plateletpheresis donors.
abstract: BACKGROUND
  The collection of platelets by apheresis is considered as a very great progress in transfusion medicine. In present era, many automated cell separation are available each model has tried to improve productivity, quality of plateletpheresis. Further various studies have been done to correlate the quality of platelet concentrates. Also, various biochemical studies have been done on plateletpheresis donors. However, safety issue with regards to post procedure levels of biochemical parameters decreased in donors undergoing plateletpheresis have been only minimally explored.
  
  
  OBJECTIVE
  Investigating Haematological and Biochemical parameters (Hematocrit value and Serum Calcium levels) pre and post in plateletpheresis donors.
  
  
  METHODS
  Sixty two healthy first time voluntary plateletpheresis donors at Apheresis unit in blood bank Bharati Vidyapeeth Deemed University Medical College & Hospital, Sangli, Maharashtra, India. Hematocrit value of plateletpheresis donors were analysed and based on mean value 43.2% considering this as standard in the present study. We categorized plateletpheresis donors in two groups (A) these having value less than 43.2% (n = 36) and Group (B) having haematocrit more than 43.3% (n = 26). Volume of ACD required for donors from both group were noted.
  
  
  RESULTS
  We observed mean of ACD infused in group A plateletpheresis donors was 347.7 ml ± 35.75 SD while group 'B' donors required mean volume ACD to be infused was 379.6 ml ± 46.24 S.D. was statistically significant (p <0.005).
  
  
  CONCLUSIONS
  Plateletpheresis induces marked metabolic effects, with sustained changes in serum calcium and haematocrit after ACD infusion, the results show, before procedure (Plateletpheresis) one must consider the haematocrit value along with serum calcium levels in Plateletpheresis donor to avoid severe symptoms of hypocalcaemia.
authors.affiliation: Tutor, Department of Biochemistry, Bharati Vidyapeeth Medical College and Hospital , Sangli, Maharashtra, India .
authors.forename: Vinayak Babasaheb
authors.initials: VB
authors.lastname: Mane
authors.affiliation: Professor and Head, Department of Biochemistry, Bharati Vidyapeeth Medical College and Hospital , Sangli, Maharashtra, India .
authors.forename: Pratap Eknath
authors.initials: PE
authors.lastname: Jagtap
authors.affiliation: Associate Professor, Department of Biochemistry, Bharati Vidyapeeth Medical College and Hospital , Sangli, Maharashtra, India .
authors.forename: Nitin Sopan
authors.initials: NS
authors.lastname: Nagane
authors.affiliation: Associate Professor, Department of Biochemistry, Bharati Vidyapeeth Medical College and Hospital , Sangli, Maharashtra, India .
authors.forename: Sushma Prakash
authors.initials: SP
authors.lastname: Dhonde
authors.affiliation: Associate Professor, Department of Biochemistry, Bharati Vidyapeeth Medical College and Hospital , Sangli, Maharashtra, India .
authors.forename: Gajanan J
authors.initials: GJ
authors.lastname: Belwalkar
authors.affiliation: Associate Professor, Department of Pathology, Bharati Vidyapeeth Medical College and Hospital , Sangli, Maharashtra, India .
authors.forename: Vaibhav Pandurang
authors.initials: VP
authors.lastname: Mane
keywords: Anticoagulant; Blood Transfusion; Hypocalcaemia