title: Genetic relationship in mulberry (MorusL.) inferred through PCR-RFLP andtrnD-trnT sequence data of chloroplast DNA.
abstract: Ten universal primer pairs of the plant chloroplast genome were used to amplify the chloroplast DNA (cpDNA) non-coding regions in eight mulberry (Morusspp.) genotypes, includingM. mongolica,M. bombycis,M. alba,M. atropurpureaandM. multicaulis. Subsequently, the polymerase chain reaction (PCR) products were digested by seven restriction enzymes and thetrnD-trnT fragment for sequence alignment, and the variations were expected to provide the genetic information for system classification. The results from this study showed that: (1) 10 cpDNA primer pairs could be used for successful amplification in the tested materials, with approximately 17.1 kb of the chloroplast genome analysed. The 152 marker loci were detected by 70 primer/restriction endonuclease combinations, among which thetrnD-trnT non-coding region digested byAluI,HinfI,MvaI andRsaI was detected by visible fragment length variation in different genotypes of the genusMorus. (2) eightMorusL. genotypes were divided into two groups based on the digesting pattern discrepancy through cpDNA. TheM. multicaulisgenotypes displayed diversity on an intraspecies level. 'Nongsang No.12' was identical with the female parent 'Beiqu No.1' (M. atropurpurea) in the surveyed sequence, but different from the male parent 'Tongxiangqing' (M. multicaulis), suggesting that the cpDNA was maternal inheritance inMorusL. (3) There were two deletion fragments (451-456 bp; 840-863bp) and six base point mutations in thetrnD-trnT region based on homologous sequence alignment. The sequence oftrnD-trnT in the cpDNA of mulberry could provide more genetic information for phylogenetic analysis and pedigree identification.
authors.affiliation: College of Life Science, Ludong University , Shandong , Yantai , P. R. China.
authors.forename: Dechang
authors.initials: D
authors.lastname: Hu
authors.affiliation: College of Life Science, Ludong University , Shandong , Yantai , P. R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.affiliation: College of Life Science, Ludong University , Shandong , Yantai , P. R. China.
authors.forename: Yan-Lin
authors.initials: YL
authors.lastname: Sun
authors.affiliation: College of Life Science, Ludong University , Shandong , Yantai , P. R. China.
authors.forename: Shumin
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Sericulture Research Institute of Shandong Province , Shandong , Yantai , P. R. China.
authors.forename: Zhaohong
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Sericulture Research Institute of Shandong Province , Shandong , Yantai , P. R. China.
authors.forename: Chuanjie
authors.initials: C
authors.lastname: Chen
keywords: Morus L.; PCR-RFLP; cpDNA; genetic analysis; trnD-trnT sequence