title: Molecular detection of field isolates of Turkey Eimeria by polymerase chain reaction amplification of the cytochrome c oxidase I gene.
abstract: Oocysts of Eimeria spp. were isolated from litter samples obtained from 30 commercial turkey farms. Genomic DNA was extracted from clean oocysts, and polymerase chain amplification of the species-specific cytochrome c oxidase subunit I (COI) gene was performed for five species of turkey Eimeria. The species tested were Eimeria adenoeides, Eimeria meleagrimitis, Eimeria meleagridis, Eimeria dispersa, and Eimeria gallopavonis. All DNA samples were positive for E. meleagrimitis, nine were positive for E. adenoeides, two were positive for E. dispersa, and none for E. meleagridis and E. gallopavonis. E. meleagrimitis occurred as a single species in 21 (70 %) of the farms while 9 (30 %) farms had a mixed species with E. meleagrimitis and E. adenoeides and 2 (7 %) were triple positive with E. meleagrimitis, E. adenoeides, and E. dispersa. This is the first account of the field prevalence of turkey Eimeria species using molecular methods.
authors.affiliation: Department of Poultry Science, University of Arkansas, Fayetteville, AR, 72701, USA.
authors.forename: T
authors.initials: T
authors.lastname: Rathinam
authors.forename: U
authors.initials: U
authors.lastname: Gadde
authors.forename: H D
authors.initials: HD
authors.lastname: Chapman
mesh_terms: D000818:Animals; D003048:Coccidiosis / Q000469:parasitology / Q000662:veterinary*; D004539:Eimeria / Q000145:classification / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D003576:Electron Transport Complex IV / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011201:Poultry Diseases / Q000469:parasitology*; D015800:Protozoan Proteins / Q000235:genetics; D013045:Species Specificity; D014422:Turkeys
chemical_list: D015800:Protozoan Proteins; D003576:Electron Transport Complex IV