title: A Novel Transgenic Mouse Line for Tracing MicroRNA-155-5p Activity In Vivo.
abstract: MicroRNA-155 (miR-155) plays significant role in various physiological processes involving both innate and adaptive immunity. miR-155 expression level changes dynamically during various immune responses. However, current approaches for miR-155 detection at the RNA level do not precisely reflect the real-time activity. Herein, we generated a transgenic mouse line (R26-DTR-155T) for determination of miR-155-5p activity in vivo by inserting miR-155-5p target sequence downstream of a reporter transgene comprising Diphtheria Toxin Receptor and TagBlue fluorescence protein. Using this approach, R26-DTR-155T mice were able to measure variation in levels of miR-155-5p activity in specific cell types of interest. The DTR expression levels were inversely correlated with the endogenous miR-155 expression pattern as detected by quantitative RT-PCR. Our data demonstrate a novel transgenic mouse line which could be useful for tracing miR-155-5p activity in specific cell types through measurement of miR-155-5p activity at single cell level.
authors.affiliation: CAS Key Laboratory for Pathogenic Microbiology (CASPMI), Institute of Microbiology, Chinese Academy of Sciences, Beijing, China; University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Krung
authors.initials: K
authors.lastname: Phiwpan
authors.affiliation: CAS Key Laboratory for Pathogenic Microbiology (CASPMI), Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Guo
authors.affiliation: CAS Key Laboratory for Pathogenic Microbiology (CASPMI), Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: CAS Key Laboratory for Pathogenic Microbiology (CASPMI), Institute of Microbiology, Chinese Academy of Sciences, Beijing, China; University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Tanyu
authors.initials: T
authors.lastname: Hu
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
authors.forename: Bhargavi M
authors.initials: BM
authors.lastname: Boruah
authors.affiliation: CAS Key Laboratory for Pathogenic Microbiology (CASPMI), Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhang
authors.affiliation: CAS Key Laboratory for Pathogenic Microbiology (CASPMI), Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Xuyu
authors.initials: X
authors.lastname: Zhou
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D019070:Cell Lineage; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D004167:Diphtheria Toxin / Q000235:genetics; D017930:Genes, Reporter / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods*; D008164:Luminescent Proteins / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D035683:MicroRNAs / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; D004167:Diphtheria Toxin; D008164:Luminescent Proteins; D035683:MicroRNAs; C532623:Mirn155 microRNA, mouse