title: [Effect of serum from patients with chronic renal insufficiency and indoxyl sulfate on lipid accumulation in macrophages in vitro].
abstract: OBJECTIVE
  To investigate the pathologies of aortic root atherosclerotic lesion in uremic apoE-/- mice and explore the effect of serum from patients with chronic renal insufficiency (CRI) and the uremic toxin, indoxyl sulfate (IS), on the expression of cholesterol transporting receptors and lipid accumulation in macrophages in vitro.
  
  
  METHODS
  The uremic apoE-/- mouse model was established by surgical operation. Frozen sections of the aortic root were collected from uremic apoE-/- mice, sham-operated apoE-/- mice and C57BL/6J mice and stained with oil red O to calculate the relative area of atherosclerotic plaque. Murine macrophage RAW264.7 cell line was treated for 12 h with different concentrations of IS or serum samples from CRI patients and healthy individuals, and the mRNA expressions of cholesterol transporting receptors (SR-A1, CD36, ABCA1, ABCG1 and SR-B1) were detected. After treatment for 24 h, the cells were induced into foam cells to determine lipid contents using oil red O staining.
  
  
  RESULTS
  The relative area of the atherosclerotic plaques in the aortic root increased significantly in uremic apoE-/- mice compared with that in sham-operated apoE-/- mice. CRI serum (5%) and IS (250 µmol/L) obviously increased the mRNA expression of CD36 and lipid accumulation in the macrophages, but did not affect the mRNA expression of other cholesterol transporting receptors.
  
  
  CONCLUSIONS
  CRI can accelerate the progression of atherosclerosis through the mechanism that IS in CRI serum promotes lipid accumulation in macrophages by enhancing the mRNA expression of CD36, which contributes to the formation of foam cells.
authors.affiliation: Department of Nephrology, Nephropathy Center, First Affiliated Hospital, Xi'an Jiaotong University College of Medicine, Xi'an 710061, China.E-mail: shenyan66@126.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Shen
authors.forename: Pei
authors.initials: P
authors.lastname: Wang
authors.forename: Juan
authors.initials: J
authors.lastname: Zhou
authors.forename: Zuyi
authors.initials: Z
authors.lastname: Yuan
authors.forename: Aiping
authors.initials: A
authors.lastname: Yin
authors.forename: Lijun
authors.initials: L
authors.lastname: Wang
mesh_terms: D000818:Animals; D001057:Apolipoproteins E; D002460:Cell Line; D005487:Foam Cells / Q000737:chemistry; D006801:Humans; D007200:Indican / Q000494:pharmacology*; D008055:Lipids / Q000737:chemistry*; D008264:Macrophages / Q000737:chemistry*; D051379:Mice; D008810:Mice, Inbred C57BL; D058226:Plaque, Atherosclerotic / Q000473:pathology; D051436:Renal Insufficiency, Chronic / Q000097:blood*
chemical_list: D001057:Apolipoproteins E; D008055:Lipids; D007200:Indican