title: Two-component system YvqEC-dependent bacterial resistance against vancomycin in Bacillus thuringiensis.
abstract: YvqEC is one of the two-component signal transduction systems that may respond to cell envelope stress and enable cells to adjust multiple cellular functions. It consists of a histidine kinase YvqE and a response regulator YvqC. In this study, we separately constructed a single gene mutant ΔyvqE and a double gene mutant ΔyvqEC in Bacillus thuringiensis BMB171 through a homing endonucleases I-SceI mediated markerless gene deletion method. We found that the deletion of either yvqE or yvqEC weakened the resistance of B. thuringiensis against vancomycin. We also identified nine operons that may be involved in the cellular metabolism regulated by YvqC. This study not only enriches our understanding of bacterial resistance mechanisms against vancomycin, but also helps investigate the functions of YvqEC.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China.
authors.forename: Shumeng
authors.initials: S
authors.lastname: Zhang
authors.forename: Yimin
authors.initials: Y
authors.lastname: Hu
authors.forename: Qingyun
authors.initials: Q
authors.lastname: Fan
authors.forename: Xun
authors.initials: X
authors.lastname: Wang
authors.forename: Jin
authors.initials: J
authors.lastname: He
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001413:Bacillus thuringiensis / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D055786:Gene Knockout Techniques; D008563:Membrane Lipids / Q000235:genetics*; D015398:Signal Transduction; D014640:Vancomycin / Q000494:pharmacology*; D020713:Vancomycin Resistance
chemical_list: D000900:Anti-Bacterial Agents; C490532:LiaRS protein, Bacillus subtilis; D008563:Membrane Lipids; D014640:Vancomycin