title: Deep Sequencing of HIV-1 in Cerebrospinal Fluid.
abstract: Using deep sequencing, human immunodeficiency virus (HIV) resistance-associated mutations were detected as minority species in the cerebrospinal fluid (CSF) of 4 patients with higher HIV type 1 RNA load in CSF than in plasma, but not in 2 patients with higher plasma viral load. Deep sequencing could help our understanding of viral escape in the central nervous system.
authors.affiliation: Department of Infection, Barts Health NHS Trust Blizard Institute, Barts & The London School of Medicine and Dentistry, Queen Mary University of London.
authors.forename: Cheuk Yan William
authors.initials: CY
authors.lastname: Tong
authors.affiliation: Directorate of Infection.
authors.forename: Sinead
authors.initials: S
authors.lastname: Costelloe
authors.affiliation: Department of Infection, Barts Health NHS Trust.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Hubb
authors.affiliation: Infection Sciences, Viapath, Guy's and St Thomas' NHS Foundation Trust.
authors.forename: Jane
authors.initials: J
authors.lastname: Mullen
authors.affiliation: Infection Sciences, Viapath, Guy's and St Thomas' NHS Foundation Trust.
authors.forename: Siobhan
authors.initials: S
authors.lastname: O'Shea
authors.affiliation: Blizard Institute, Barts & The London School of Medicine and Dentistry, Queen Mary University of London Department of Neurosciences, Barts Health NHS Trust.
authors.forename: Monica
authors.initials: M
authors.lastname: Marta
authors.affiliation: Department of Genitourinary Medicine, Guy's and St Thomas' NHS Foundation Trust.
authors.forename: Ranjababu
authors.initials: R
authors.lastname: Kulasegaram
authors.affiliation: Infection and Immunology, Barts Health NHS Trust Department of Medicine, Mildmay Hospital UK, London, United Kingdom.
authors.forename: Simon
authors.initials: S
authors.lastname: Rackstraw
mesh_terms: D000328:Adult; D002555:Cerebrospinal Fluid / Q000821:virology*; D024882:Drug Resistance, Viral; D005260:Female; D014644:Genetic Variation; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D012189:Retrospective Studies; D019562:Viral Load
keywords: CNS penetration; deep sequencing; minority species; viral escape