title: Comparison of inhibitory activity of bioactive molecules on the dextransucrase from Streptococcus mutans.
abstract: The effect of chitosan with different molecular weights and other natural substances on dextransucrase (DSase) activity from a representative oral pathogen Streptococcus mutans was elucidated. Among other bioactive substances, amino-monosaccharides such as glucosamine, mannosamine, and galactosamine exerted the enzyme inhibitory activity over 95% of DSase. The specified hydrolysates derived from the hydrolysis of high molecular weight chitosan (HMWC) designated to CTSN, CTSN-P, CTSN-B, and CTSN-S with different molecular weights ranging from 3 to 8 kDa showed the similar inhibitory activity toward DSase. Also, the hyaluronic acid (MW 8.9 kDa), sulfated chitin, and amino-monosaccharides demonstrated the significant activity, CTSN, CTSN-P, CTSN-B, and CTSN-S are of potent bioactive substances that can be prepared in the cheapest way compared with other molecules tested available for antibacterial agent useful for human oral health.
authors.affiliation: Department of Life Sciences, Gachon University, Seongnamdaero 1342, Seongnam-si, Gyeonggi-do, 461-701, South Korea.
authors.forename: Choon Geun
authors.initials: CG
authors.lastname: Lee
authors.forename: Jae Kweon
authors.initials: JK
authors.lastname: Park
mesh_terms: D000890:Anti-Infective Agents / Q000378:metabolism*; D048271:Chitosan / Q000737:chemistry / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000378:metabolism*; D005688:Galactosamine / Q000378:metabolism; D005944:Glucosamine / Q000378:metabolism; D005964:Glucosyltransferases / Q000037:antagonists & inhibitors*; D006595:Hexosamines / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D008970:Molecular Weight; D013295:Streptococcus mutans / Q000201:enzymology*
chemical_list: D000890:Anti-Infective Agents; D004791:Enzyme Inhibitors; D006595:Hexosamines; C008322:mannosamine; D005688:Galactosamine; D048271:Chitosan; D005964:Glucosyltransferases; C020025:dextransucrase; D005944:Glucosamine