title: Manitoba Lingonberry (Vaccinium vitis-idaea) Bioactivities in Ischemia-Reperfusion Injury.
abstract: Evidence for the efficacy of dietary interventions in protecting against cardiovascular disease has grown significantly, with flavonoids and anthocyanins receiving special attention. Lingonberry (Vaccinium vitis-idaea L.) is a good source of these compounds, and this study examined the protective effects of wild lingonberry found in Manitoba, Canada, against ischemia-reperfusion (IR) injury. Manitoba lingonberry contained 3793 ± 27 mg gallic acid equiv, 120,501 ± 7651 μmol trolox equiv, and 575 ± 20 mg cyanidin-3-glucoside equiv per 100 g dry weight, which correspond with high total phenolic content, antioxidant activity, and anthocyanin content, respectively. A complete methanolic extract and both anthocyanin-rich and phenolic-rich fractions inhibited apoptosis in H9c2 cells during simulated IR. Lingonberry extract and fractions significantly inhibited several markers of apoptosis induced by IR, including nuclei condensation, caspase-3 activation, and MAP kinase signaling. These results provide the first analysis of Manitoba lingonberry and highlight the mechanistic importance of dietary berry compounds for cardiovascular health.
authors.affiliation: §Physiology and Pathophysiology, University of Manitoba, 432 Basic Medical Sciences Building, 745 Bannatyne Avenue, Winnipeg, Manitoba, Canada R3E 0J9.
authors.forename: Cara K
authors.initials: CK
authors.lastname: Isaak
authors.forename: Jay C
authors.initials: JC
authors.lastname: Petkau
authors.affiliation: §Physiology and Pathophysiology, University of Manitoba, 432 Basic Medical Sciences Building, 745 Bannatyne Avenue, Winnipeg, Manitoba, Canada R3E 0J9.|⊗Animal Science, University of Manitoba, 201-12 Dafoe Road, Winnipeg, Manitoba, Canada R3T 2N2.
authors.forename: Karmin
authors.initials: K
authors.lastname: O
authors.affiliation: ⊥Atlantic Cool Climate Crop Research Centre, Agriculture and Agri-Food Canada, St. John's, Newfoundland, Canada A1E 0B2.
authors.forename: Samir C
authors.initials: SC
authors.lastname: Debnath
authors.affiliation: §Physiology and Pathophysiology, University of Manitoba, 432 Basic Medical Sciences Building, 745 Bannatyne Avenue, Winnipeg, Manitoba, Canada R3E 0J9.
authors.forename: Yaw L
authors.initials: YL
authors.lastname: Siow
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D005638:Fruit / Q000737:chemistry; D006801:Humans; D007511:Ischemia / Q000601:surgery; D008350:Manitoba; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000201:enzymology / Q000235:genetics / Q000503:physiopathology; D029801:Vaccinium vitis-idaea / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D020928:Mitogen-Activated Protein Kinases; D053148:Caspase 3
keywords: H9c2; JNK; Vaccinium vitis-idaea; anthocyanin; antioxidant; apoptosis; ischemia-reperfusion; lingonberry