title: The rice RCN11 gene encodes β1,2-xylosyltransferase and is required for plant responses to abiotic stresses and phytohormones.
abstract: Seed germination rates and plant development and growth under abiotic stress are important aspects of crop productivity. Here, our characterization of the rice (Oryza sativa L.) mutant reduced culm number11 (rcn11) showed that RCN11 controls growth of plants exposed to abnormal temperature, salinity and drought conditions. RCN11 also mediates root aerenchyma formation under oxygen-deficient conditions and ABA sensitivity during seed germination. Molecular studies showed that the rcn11 mutation resulted from a 966-bp deletion that caused loss of function of β1,2-xylosyltransferase (OsXylT). This enzyme is located in the Golgi apparatus where it catalyzes the transfer of xylose from UDP-xylose to the core β-linked mannose of N-glycans. RCN11/OsXylT promoter activity was observed in the basal part of the shoot containing the shoot and axillary meristems and in the base of crown roots. The level of RCN11/OsXylT expression was regulated by multiple phytohormones and various abiotic stresses suggesting that plant specific N-glycosylation is regulated by multiple signals in rice plants. The present study is the first to demonstrate that rice β1,2-linked xylose residues on N-glycans are critical for seed germination and plant development and growth under conditions of abiotic stress.
authors.affiliation: Department of Agro-environmental Science, Obihiro University of Agricultural and Veterinary Medicine, 2-11 Nishi, Inada, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Sho
authors.initials: S
authors.lastname: Takano
authors.affiliation: Department of Agro-environmental Science, Obihiro University of Agricultural and Veterinary Medicine, 2-11 Nishi, Inada, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Shuichi
authors.initials: S
authors.lastname: Matsuda
authors.affiliation: Department of Agro-environmental Science, Obihiro University of Agricultural and Veterinary Medicine, 2-11 Nishi, Inada, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Funabiki
authors.affiliation: Graduate School of Advanced Life Science, Laboratory of Advanced Chemical Biology, Hokkaido University, Kita 10 Nishi 8, Kita-ku, Sapporo, Hokkaido 060-0810, Japan.
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Furukawa
authors.affiliation: Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa, Nagoya, Aichi 464-8601, Japan.
authors.forename: Takaki
authors.initials: T
authors.lastname: Yamauchi
authors.affiliation: Department of Food Science, Obihiro University of Agricultural and Veterinary Medicine, 2-11 Nishi, Inada, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Tokuji
authors.affiliation: Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa, Nagoya, Aichi 464-8601, Japan.
authors.forename: Mikio
authors.initials: M
authors.lastname: Nakazono
authors.affiliation: Graduate School of Advanced Life Science, Laboratory of Advanced Chemical Biology, Hokkaido University, Kita 10 Nishi 8, Kita-ku, Sapporo, Hokkaido 060-0810, Japan.
authors.forename: Yasuro
authors.initials: Y
authors.lastname: Shinohara
authors.affiliation: Graduate School of Agriculture, Hokkaido University, Kita 9 Nishi 9, Kita-ku, Sapporo, Hokkaido 060-8589, Japan.
authors.forename: Itsuro
authors.initials: I
authors.lastname: Takamure
authors.affiliation: Department of Agro-environmental Science, Obihiro University of Agricultural and Veterinary Medicine, 2-11 Nishi, Inada, Obihiro, Hokkaido 080-8555, Japan. Electronic address: kiyoaki@obihiro.ac.jp.
authors.forename: Kiyoaki
authors.initials: K
authors.lastname: Kato
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D012275:Oryza / Q000235:genetics / Q000502:physiology*; D010430:Pentosyltransferases / Q000235:genetics* / Q000378:metabolism; D010937:Plant Growth Regulators / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D018520:Plant Shoots / Q000378:metabolism; D013312:Stress, Physiological
chemical_list: D010937:Plant Growth Regulators; D010940:Plant Proteins; D000040:Abscisic Acid; D010430:Pentosyltransferases; C408586:beta 1,2-xylosyltransferase
keywords: Abiotic stresses; Low temperature; N-Glycan; Rice; Root aerenchyma formation; β1,2-Xylosyltransferase