title: Action of an endo-β-1,3(4)-glucanase on cellobiosyl unit structure in barley β-1,3:1,4-glucan.
abstract: β-1,3:1,4-Glucan is a major cell wall component accumulating in endosperm and young tissues in grasses. The mixed linkage glucan is a linear polysaccharide mainly consisting of cellotriosyl and cellotetraosyl units linked through single β-1,3-glucosidic linkages, but it also contains minor structures such as cellobiosyl units. In this study, we examined the action of an endo-β-1,3(4)-glucanase from Trichoderma sp. on a minor structure in barley β-1,3:1,4-glucan. To find the minor structure on which the endo-β-1,3(4)-glucanase acts, we prepared oligosaccharides from barley β-1,3:1,4-glucan by endo-β-1,4-glucanase digestion followed by purification by gel permeation and paper chromatography. The endo-β-1,3(4)-glucanase appeared to hydrolyze an oligosaccharide with degree of polymerization 5, designated C5-b. Based on matrix-assisted laser desorption/ionization (MALDI) time-of-flight (ToF)/ToF-mass spectrometry (MS)/MS analysis, C5-b was identified as β-Glc-1,3-β-Glc-1,4-β-Glc-1,3-β-Glc-1,4-Glc including a cellobiosyl unit. The results indicate that a type of endo-β-1,3(4)-glucanase acts on the cellobiosyl units of barley β-1,3:1,4-glucan in an endo-manner.
authors.affiliation: a Life Science Materials Laboratory, Research and Development Division , ADEKA Corporation , Tokyo , Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Kuge
authors.affiliation: b Division of Life Science, Graduate School of Science and Engineering , Saitama University , Saitama , Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Nagoya
authors.affiliation: c Department of Biochemistry , University of Cambridge , Cambridge , UK.
authors.forename: Theodora
authors.initials: T
authors.lastname: Tryfona
authors.affiliation: b Division of Life Science, Graduate School of Science and Engineering , Saitama University , Saitama , Japan.
authors.forename: Tsunemi
authors.initials: T
authors.lastname: Kurokawa
authors.affiliation: b Division of Life Science, Graduate School of Science and Engineering , Saitama University , Saitama , Japan.
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Yoshimi
authors.affiliation: b Division of Life Science, Graduate School of Science and Engineering , Saitama University , Saitama , Japan.|d Global Research Cluster , RIKEN , Saitama , Japan.
authors.forename: Naoshi
authors.initials: N
authors.lastname: Dohmae
authors.affiliation: a Life Science Materials Laboratory, Research and Development Division , ADEKA Corporation , Tokyo , Japan.
authors.forename: Kazufumi
authors.initials: K
authors.lastname: Tsubaki
authors.affiliation: c Department of Biochemistry , University of Cambridge , Cambridge , UK.
authors.forename: Paul
authors.initials: P
authors.lastname: Dupree
authors.affiliation: b Division of Life Science, Graduate School of Science and Engineering , Saitama University , Saitama , Japan.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Tsumuraya
authors.affiliation: b Division of Life Science, Graduate School of Science and Engineering , Saitama University , Saitama , Japan.|e Institute for Environmental Science and Technology , Saitama University , Saitama , Japan.
authors.forename: Toshihisa
authors.initials: T
authors.lastname: Kotake
mesh_terms: D002473:Cell Wall / Q000737:chemistry; D005936:Glucans / Q000737:chemistry*; D006026:Glycoside Hydrolases / Q000737:chemistry* / Q000378:metabolism; D001467:Hordeum / Q000737:chemistry / Q000201:enzymology*; D006868:Hydrolysis; D009844:Oligosaccharides / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013379:Substrate Specificity
chemical_list: D005936:Glucans; D009844:Oligosaccharides; C040088:1,4-glucan; D006026:Glycoside Hydrolases
keywords: MALDI-ToF/ToF-/MS/MS; barley; cellobiosyl unit; endo-β-1,3(4)-glucanase: β-1,3:1,4-glucan